LOCUS       DAOSXM010000001       103831 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2111
            NODE_97_length_103831_cov_11.570141, whole genome shotgun sequence.
ACCESSION   DAOSXM010000001 DAOSXM010000000
VERSION     DAOSXM010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864178
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 103831)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 103831)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:24:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,261
            CDSs (total)                      :: 2,229
            Genes (coding)                    :: 2,218
            CDSs (with protein)               :: 2,218
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 3 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..103831
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97_length_103831_cov_11.570141"
                     /isolate="AMR_MDS_2111"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(43..987)
                     /locus_tag="PLQ68_00005"
     CDS             complement(43..987)
                     /locus_tag="PLQ68_00005"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HXK71062.1"
                     /translation="MRKNTLLILLICAITLLSSCDNTTVPPSFTDTVTPDITVTLPPS
                     ESETDQPSQTQAEPSQTFETIEPSPTVIPPNSTYIDYYDLYIDLTVTDELSREQISWG
                     EKSNKTEVMEKYNVRTSMDPEKKEIYLTFNLGYEHVRGNTEKLLELLKQRDILATFFL
                     VGEFIEIFPEKVKAIYDDGHTLGAHSYYHIPPSYTMTDEEYIDDFIKTEKLLEIALKD
                     DYERMRYYRPIIGEFCERDLYIAQSLGYTTVLFDATYSDWNRTVINGAEFAYNELTQD
                     VHEGAVYQLHIVTPDNIEALPVFLDELINDGWTFKIFE"
     gene            complement(993..2210)
                     /locus_tag="PLQ68_00010"
     CDS             complement(993..2210)
                     /locus_tag="PLQ68_00010"
                     /inference="COORDINATES: protein motif:HMM:NF022702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3048 domain-containing protein"
                     /protein_id="HXK71063.1"
                     /translation="MKHKRQISNILRKTIIISLIIVISILFSSCGSYFEAVGNIAESP
                     TLPSKTPRPSVTPTQTEQPSESVEPTETPTESVTPTEEPSATIDPSLVLAVNPLTGKS
                     EYMHEFALDVKPVAIILENKRQSLPQAGISEADIVIEMLVEYDISRFMAIYQDPYNVE
                     KIGAVRSLRSYFTEMALAFDSYIFHYGFSEYGEENAKKDLADRGLTTINGIFGEDNGY
                     PFWRDSDRIASGMESVHCVVTAGQNVLRSMQNISTEKIEKTEQKNIFNFSTQSSTKEG
                     NDANKVYLTGIYISSPYFIYDNNKSEYTRYQFADKHIDGNSGEAISVENLFLLKMDTR
                     YLGDASEHVAITTYGSGSGYYINDGKYIEIQWSRETDSSEFEFTYNNEPLQVAQGNSW
                     ISCIPTDADITIR"
     gene            complement(2277..3545)
                     /locus_tag="PLQ68_00015"
     CDS             complement(2277..3545)
                     /locus_tag="PLQ68_00015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK71064.1"
                     /translation="MSTKENKPTNSLIEKLNKSYLILGAVFLVAFITRVFIAGAVMGH
                     LTDMGCFSYWGEQAYNDGLLEFYNGSYYCDYPPLYIYILWFIEFAREALGLTFAFPAH
                     IILLKMPAIIFDIMTAYLVYKIAKTRFSETTSIILSAAVALNIAYILNSAAWGQIDSI
                     LTFFIALTAYNIMNNKLEFATVAYVIAVLLKPQAFIFAPILIFAYIKSKNTKKVLISL
                     LIGLALFVVVILPFAIRQEPLWIFSLYFGTMGQYAYATVNAYNIYALLGLNWVSDSEV
                     MFLFAYKIYGYVAILGICALTTIAFIKGNKMPGFTFATAGLLMTVVFAFATKMHERYL
                     FPSILLVLAAYIFSNDKRFLYIHILQSITVFANCAYILYMNFNGGYDLFSPYVAIVSA
                     VTIFTTLWYLRVYFQLYILNQDNIPLKYLT"
     gene            complement(3514..4017)
                     /locus_tag="PLQ68_00020"
     CDS             complement(3514..4017)
                     /locus_tag="PLQ68_00020"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004614090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HXK71065.1"
                     /translation="MNLIVAVDENWGIGYMGKIQWHIKEDMAYFKNKTMDKTVIMGRR
                     TLDSFKNGKPLKNRKNIVLTRDMDFKRDDVIVVNTIEQALNKIENENSSDVYVIGGES
                     IYQALLPYCDKAYVTKVHGIFKADKHFINLDNEPQWTAVHISDRLMTESGVHITFYEY
                     QRKQTDQ"
     gene            complement(4014..4844)
                     /gene="thyA"
                     /locus_tag="PLQ68_00025"
     CDS             complement(4014..4844)
                     /gene="thyA"
                     /locus_tag="PLQ68_00025"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009002428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HXK71066.1"
                     /translation="MSKADDIFKNMCRDIIDNGFSDKDLDVRPKWLDGVPAHTVKKFC
                     VVNRYDLSEEFPILTLRPTRFKGSIDEILWIWQKKSNNINDLNSKIWDAWADENGSIG
                     KAYGYQLSVKHKYNEGMFDQVDRVLYDLKNNKASRRIMTNIYVHADLHEMNLYPCAYS
                     MTFNVSGNILNGILNQRSNDILIANNWNVVQYAVLMHIIAQASGLIAGELVHVIADAH
                     IYDRHIPLVEELLKRPAYPAPKFWINPEITNFYDFTVDDFKLIDYKKNPQIKDIEVAV
                     "
     gene            complement(4902..5411)
                     /locus_tag="PLQ68_00030"
     CDS             complement(4902..5411)
                     /locus_tag="PLQ68_00030"
                     /inference="COORDINATES: protein motif:HMM:NF019099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HXK71067.1"
                     /translation="MSKSKKITISAIFTALAISLSIAEGYIPFSAIAPGVKLGLANVV
                     PLTLLYVLGPVYAVAVQAVRIIIAGLLRGNFVTFLFSFFGGMASILVCVLIKKQKKIQ
                     FSVIGLSIIGAIVHNLAQFITALVILRNINIFWYIAILSVFAVVTGILTGIIAKYVIK
                     ALKGYYKGI"
     gene            complement(5408..5779)
                     /locus_tag="PLQ68_00035"
     CDS             complement(5408..5779)
                     /locus_tag="PLQ68_00035"
                     /inference="COORDINATES: protein motif:HMM:NF018685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HXK71068.1"
                     /translation="MKKADIALIAILICVFVIFISIYFLTDNKDDSILIIQQDNEIVY
                     RKPIDEDNIVNIYDDNGNLINTVTIKDGKAYMSFATCPNKDCMHMGYIQSGSRKQIAC
                     LPNKVLIYLTEDNVIVDGITR"
     gene            complement(5795..6691)
                     /locus_tag="PLQ68_00040"
     CDS             complement(5795..6691)
                     /locus_tag="PLQ68_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HXK71069.1"
                     /translation="MEYKDYYKVLGVEKKDDKDTIKKAYRKLAKKYHPDNNPNNKRAE
                     ERFKEINEAYEVLSDDEKRKRYDTLGSNGFNGKGFDPSSFGNQYSYDFNRGSGFSQSN
                     DFSDFFNAFFADLGMGGMGADPFSSSSRKSARRSPSQPDSEASMTISIEDALSGREMV
                     VSINNKKVNLKIPKGIRPGQKIKMKGQGQNGGDLFIKFDIQNTATLSLEGDDLIKTAD
                     VYPWQAYFGSQVQINMPDTTLKVTVPQKVKGGSRIRVRGKGYYKKDGSRGDLYINVNI
                     VNPSNMSTADEQIYKELLNKYQ"
     gene            complement(7126..7608)
                     /locus_tag="PLQ68_00045"
     CDS             complement(7126..7608)
                     /locus_tag="PLQ68_00045"
                     /inference="COORDINATES: protein motif:HMM:NF016363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HXK71070.1"
                     /translation="MSVFDKFLTEFLGFEQAAPEKQQVAPVTRDGNGKTRPSVQVSPI
                     VDRIDALDQIASKKVVPMERSTGILNVMYPESFEETKRVCDSLKSGRAVIVNLEQIEK
                     ELAQRIVDFLSGTVYTLNGAIRKLSDKILVVAPSGYHIDDEKQDLSKLADTKTRTFAN
                     "
     gene            complement(7653..8318)
                     /locus_tag="PLQ68_00050"
     CDS             complement(7653..8318)
                     /locus_tag="PLQ68_00050"
                     /inference="COORDINATES: protein motif:HMM:NF013345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HXK71071.1"
                     /translation="MINYVAENIYKIKKQLNNDDILIVAVSKTVSCETAALALVVPGI
                     ELGENRVQEFLKKYDYFQGKAKIHLIGHLQKNKVKYIVGKTVLIHSVDSEELLDEIQK
                     RSSQQQVVTNVLLQIKIFKDDNKFGADEKDIEKLISHNENNPNVKIKGLMTMAPFTQD
                     NELISHTFRKLHDIFVDISKKSFYNTDMVYLSMGMSNDYILAAKEGANILRVGSAIFK
                     DKP"
     gene            complement(8330..9598)
                     /locus_tag="PLQ68_00055"
     CDS             complement(8330..9598)
                     /locus_tag="PLQ68_00055"
                     /inference="COORDINATES: protein motif:HMM:NF024112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HXK71072.1"
                     /translation="MIKGKKKEKKDKKDKNTKAIIRLMIVIAFFLFVILVSRMAINYY
                     SSIVKKDIAYNGKLIDQINISGLVLYEYDSYDAVEGYSLIKTYEEGSRIRVNSLIGSL
                     VSTNNPLYNDLVEINDQIQKKQQLYEINTGIISEDIKIIDNQVRKSLLSFSSVLSSQT
                     LSDVQIHKESIEALNEYLNDVKSGNNEKQQQLDELYVQQQQLKQAMASDVFDVVAVNS
                     GYLSYNMQYNQQYHTYEQMKSVYPTHLHDEMSGDNRLESSTAVKVATSKKFHIAVVLS
                     SSDAARIKDIEDLKININTSGMDVYIDISKVIIGNSEDNETVVFFEVDNGLSQLVSLG
                     KFDGLLVIKEYEGLKIPISAIYNYKYSSFQNMQVALVKGISVKFVYIDVLYTDGIYAI
                     IKDIDEDYSFKVYDYYILDPYSVSDGDVVN"
     gene            complement(9603..10862)
                     /locus_tag="PLQ68_00060"
     CDS             complement(9603..10862)
                     /locus_tag="PLQ68_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine gamma-lyase family protein"
                     /protein_id="HXK71073.1"
                     /translation="MIKGFDIDKKLISLADDVQQLCDNQFKELSDTAETNQVRITKVF
                     QDRRVSDAYFAETTGYGYSDRGRDLLDEVYADIFNTEDALVRESIASGTSAISLAMSS
                     NVRPGDEIIFANGMPYDTLRSTVGITKATGSLAEYGITYRIIEHIEDKIDETAVMNAI
                     SDKTKMIFIQRSRGYSLRPPVSKEQFISISKKVKAVRKDIILFVDNCYGEFVCDEEPT
                     HWGMDMAAGSLIKNPGGGLCRSGGYIVGTKECILNASARLYAPGLLKEVGATNNKRLM
                     FQGLYMAPYTVMESLKGAVFTSLLFEKLGFKSYPEYKEDRQDIIQLVIFKNKEQLLEF
                     CKGVQAGSPIDSHVTPYPWDMPGYDDQVVMAAGTFIQGASIEFSADAPLREPYIAYMQ
                     GGLTYTQVKLGVLKACDMMLKKGILNI"
     gene            complement(10859..12085)
                     /locus_tag="PLQ68_00065"
     CDS             complement(10859..12085)
                     /locus_tag="PLQ68_00065"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HXK71074.1"
                     /translation="MNTIQKKEQEDTYYINEPNIVAKVLLRGIIKGGFRIVLNLRSNK
                     SSDIDNLEGSTILLGNHVSAIDPFVMGAFVTKKWVHYLTSSSYFEYPILKKILKYFGA
                     IPKRQGMSDIRSTKMAINVLKRNGTIGLFPEGARTIDGSMLPFNNSAAKLIKQSNSNV
                     VISHLNGASISLPRWYRGCSRGFRRGRIDHKSFVLFTNQQVRSMSIDDIDSKIRIALS
                     YNEYEWQRRKHIKFHSRSMAEGMHAILHKCPKCLQDFSMHTKKNLLYCTDCDNTAVID
                     RYGLINPQSEDDIVYQDMIAWRKWQNTYLAKEIQKDDFKMEFEGSLSVKDSYGKVIKI
                     YPQCRLTVTKENVSYSCSEDDIILPFKSIADAVCDYSLRFEINQEHNSYQFTPVNGQT
                     VIKISDAIKYIKGILE"
     gene            12220..13116
                     /locus_tag="PLQ68_00070"
     CDS             12220..13116
                     /locus_tag="PLQ68_00070"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HXK71075.1"
                     /translation="MKNSNDVYIELIVSQQSHKRRLSSFLRKEMGISGNCIRRAKNFD
                     NIYVDKKPQHTDYILSEGQLVTIVVPDKDTKSVILSSDMNLNILYDDEYLIAVDKPYG
                     VLCHPIMSVDEDTIANGLKHIYPGMGIHPISRLDRDTTGIVMYAKNSIVHHKMSLIDI
                     NKKYIGMVLNSPPVTEGTIDMPIARVPVSTMLRQFDPSGKESITHYSTIFSKKSASIL
                     LFTLETGRTHQIRVHCKEGGFPIIHDTLYGKPSDYDQYISRQALHSYETSFIHPFTNE
                     KICIKSDLHDDMLNLEKAIREK"
     gene            13154..13882
                     /gene="recO"
                     /locus_tag="PLQ68_00075"
     CDS             13154..13882
                     /gene="recO"
                     /locus_tag="PLQ68_00075"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HXK71076.1"
                     /translation="MEYLKAKGIVCKISDYKESDKILTLLTYEKGKIQVIAKGVKKKG
                     AALAHAARLFYCGNFEFICSNNIPILTGASQIQDFFGLTHKMETLYYASHFMNLCSYF
                     IQEEQPSHDLMSLLLNSLYLLMREEADPMLLTSIIQFRIASLEGSAPITDFCVECQKP
                     LDNDCSFCFSIDNDGISCCNDGTCISHAIFKAINHICNADASKIYFFNIPKADLPVLY
                     ELSCRYIEKIAGKHFEILDWIKEL"
     gene            complement(13908..14801)
                     /gene="era"
                     /locus_tag="PLQ68_00080"
     CDS             complement(13908..14801)
                     /gene="era"
                     /locus_tag="PLQ68_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HXK71077.1"
                     /translation="MTFKSGFVSVIGRPNVGKSTLINAITKQRISIVTPKPQTTRRNI
                     KAIYTDESSQIIFLDTPGIHEPKTRLGEYMVTAAKSTFTETDVIVFVTAPKKQHEIPY
                     EDVIILELLSNIDIPKICVINKCDTIDQPTARWYTEQYSKKVKFDQIMAISAQEDFNV
                     EQLKQMIVSYLEEGPMYYDEDEITDQTMRQMAEDIIREKLLFLLSEEVPHGTAVEIEL
                     FKKREDKDLYDIEAVIYCDKDSHKGIVIGKSGSMLKRIGTEARKSMEHTFDFKINLKL
                     WVKVKNDWRNDNRMLNTLGYK"
     gene            complement(14803..15213)
                     /gene="cdd"
                     /locus_tag="PLQ68_00085"
     CDS             complement(14803..15213)
                     /gene="cdd"
                     /locus_tag="PLQ68_00085"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0008655 - pyrimidine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HXK71078.1"
                     /translation="MTDKYDALKLAAQNSIDNAYSPYSKIKIGAAVLASSGKIYTGAN
                     IENASYGATICAERTAIVKAMSENEKYISAIAITGDINDYAFPCGICRQVMSEFMSKD
                     AIVFVSGKDREWKTFKLKELLPNSFDFYDDIKGR"
     gene            complement(15203..15694)
                     /gene="ybeY"
                     /locus_tag="PLQ68_00090"
     CDS             complement(15203..15694)
                     /gene="ybeY"
                     /locus_tag="PLQ68_00090"
                     /inference="COORDINATES: protein motif:HMM:NF014216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HXK71079.1"
                     /translation="MKTRIDIEGLNDRTKQFEEPVKKVLDKILQANSIDDDNLCLSVF
                     IEFVDDEYIKQINFEQRDIDSVTDVLSFPATDSSDGKIVYDKYDIDYENNELFLGDIL
                     ICESKVFSQAESYGHSEIRECVFLASHGLLHLLGYDHQDEKQENLMQEFQENILKELG
                     YDR"
     gene            complement(15678..17846)
                     /locus_tag="PLQ68_00095"
     CDS             complement(15678..17846)
                     /locus_tag="PLQ68_00095"
                     /inference="COORDINATES: protein motif:HMM:NF019317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HXK71080.1"
                     /translation="MSRSKINKRDLLKNRLYNVLILLLAAIIAVLGIFQKVSDTGHMI
                     TSDNVASTDIVASFDYYDKEATLSKAEAAKQAVEPIAVRISTASVALYENIIGFFDVT
                     DVYRGQVKGLSMTSPQYNTALNSLINQYTSIFYSLGVDIPNNLARAILRDVNDTTYDS
                     YKTVIRSKFAEFMDDYIHEDNLIEYQEKFVTLVNESVTDSNLNELTDILKNYFVKVNS
                     RIDQIATLMEKERVYQDVIDNSPVVVFAGSVLYEKGDKLQSDDIKTLSEMGVIKVANE
                     LYMYIGAAVLFIMCILASLASKKICGHTMKLQPKSIFILVLLFFMVYIPALFVPSEYY
                     MYIPYFILPMMLAILISDNTSYVFSCPMAVFYCYIVGGDIVYLTILLLGSIATIYIVR
                     NTSARIRLAISGIALGVINMVIYMAYMLITTDITINMPYTQLIALFISSMGAALLCLG
                     LLPVFEAFFNIVTPFKLMELASPTRPLLKRLMIEAPGTYHHSLMVGNLAEEAAEAIGA
                     NGLLARVGAYYHDVGKLNRPAFFGENQSSKTGNPHNTMTPQLSATIITSHTNEGVDLA
                     KQAKLPPSIRDIIQEHHGTTQVQYFYHKAQSVSDEPIMEEDYRYSGPKPRTKESACVM
                     LADSCEATIRSMETTNIIEIEKTIRKVIKSKRDDGQFDQCDLTLKDLDEILFAFMKVF
                     TGYFHQRVKYPNQTDKDIAAAKTLSLEEKEEEKEDNEDEN"
     gene            complement(17839..18807)
                     /locus_tag="PLQ68_00100"
     CDS             complement(17839..18807)
                     /locus_tag="PLQ68_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006355379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HXK71081.1"
                     /translation="MERLINFENLSDMMSVFGSSDSNLKVIENMMNVSVIVRGSEVRL
                     KGEVEEVNRAEKLITRLIDMSQAGEDINKQNILYLADLINQGKDESFYLPSGYICLNA
                     SGAPIKAKTLGQKKYTDAIQKNGITFGVGPAGTGKTFLAVACAVTAFRAKSVKRIILT
                     RPAVEAGEKLGFLPGDLQSKIDPYLRPLYDALFELMGPDNVARNKEKGLIEIIPLAYM
                     RGRTLDDAFIILDEAQNTTEEQMKMFLTRMGFGSKMVINGDITQIDLPREKKSGLITA
                     IHILKNIDDIGIINLTFKDVVRHELVQKIINAYERAENKKKVNKNE"
     gene            complement(18893..19282)
                     /locus_tag="PLQ68_00105"
     CDS             complement(18893..19282)
                     /locus_tag="PLQ68_00105"
                     /inference="COORDINATES: protein motif:HMM:NF014762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HXK71082.1"
                     /translation="MSGLTQSMENYLRSIYELSYANKGVRLTDLAEKMKVKKASANNA
                     VMKLTKMGYCVQLKYEPIFLTDAGIEAVKAATGKHIIILKFLKDVLSVDNQTAETETT
                     KIEHHLSTSTVYAMHQLHTKLKSKPDN"
     gene            19510..20733
                     /gene="pepT"
                     /locus_tag="PLQ68_00110"
     CDS             19510..20733
                     /gene="pepT"
                     /locus_tag="PLQ68_00110"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014932510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HXK71083.1"
                     /translation="MKVHERFLDYVRFDTTSDPNSKTCPSTPNQMEFARYLVEDMKKI
                     GISNAYVDEFGYVYGFIDASEGFENVESIGFIAHMDTSDAVSGKDINPCIISDYTGDD
                     VVLNEKLNIIMEVDKYPHLKNYTGHDLIVTDGTTLLGVDDKAGIAEILEMADILINDP
                     SKKHGRICIAFTPDEEIGRGADLFDVKKFNAAYAYTVDGGPLGDIQYENFNAASTNVR
                     ITGLSVHPGGAKNIMKNAILMAYEFNSLLPHCETPAHTEKREGFYHLHNISGSEESAH
                     MSYILRDHDYEKLQQKKDRMKLAAEYINSIYGENSVTLEISDTYLNMAEMLKPHMHII
                     ERANRAYEALGYTPTTHAIRGGTDGARLSYMGLLCPNIATGGHNAHGRFEYVSINEMN
                     KMVSVLVHMACDINQ"
     gene            complement(20723..21835)
                     /gene="prfB"
                     /locus_tag="PLQ68_00115"
     CDS             complement(20723..21835)
                     /gene="prfB"
                     /locus_tag="PLQ68_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014314165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HXK71084.1"
                     /translation="MIDYGAIRNELNDIKAESAKLIKSLDSEQSRNEIENLEHQTMQE
                     AFWQDNENSQKVLQRIKVLKDRSAKAGDLKTKLEDCFVLLEFSEENPEDQELENELVT
                     AKEDLKNAYIQIRMYSLLNGTNDKNNAYLTLHSGAGGTEACDWVEMLFRMYSRYFESK
                     GFKATVTDYLDGDGAGIKSVTVYVEGEYAYGYLRGESGVHRLVRISPFDSAGRRHTSF
                     ASCEVIPEVSHDIEVKIKTEDLRIDTFRSSGKGGQHVNKTDSAIRITHLPTNIVVACQ
                     SERSQHQNKESAMRMLMSKLEEIKEREHKEKIEDLKGEQMDIGWGSQIRSYVFCPYTM
                     VKDHRTNYETGDVQSVMDGNLDAFVNEYLTQNPTID"
     gene            complement(21832..23001)
                     /locus_tag="PLQ68_00120"
     CDS             complement(21832..23001)
                     /locus_tag="PLQ68_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003374662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HXK71085.1"
                     /translation="MKKPKDFIRSEVEKTPPSGIRKYFDMLHGMDDVISLGVGEPDFT
                     TPWQIRESAIHALEKGRTHYTSNNGILQLREALSLMLHQKYNLNYDPKDQIIITVGAS
                     EAIDIAIRSIAGCGDEVIIPQPCFVAYKGCVNFTGAKAVPLQLSEDNCFKLTVDQLEK
                     CITDKTKAIILAYPNNPTGAVMTKEELAPIADYLSKKDIMVISDEIYAELTYGMKHTS
                     FAEFENMHDKVIYVNGFSKAYSMTGWRLGYVCAHRDIISQMIKIHQYAIMCAPSFVQY
                     AAIDALKQCDGDVINMRNEYDARRRYIYNGLKNIGIDCFEPKGAFYVFPNIRKTGLTS
                     EQFCDRLLMEHKVLVVPGTAFSDAGEGFFRATYASSLENIKEALKRIERFMKTLK"
     gene            complement(22998..23474)
                     /locus_tag="PLQ68_00125"
     CDS             complement(22998..23474)
                     /locus_tag="PLQ68_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016208495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HXK71086.1"
                     /translation="MDILELLKQDARLTAQELAVMTGKTVEEVERVIKQYEQDKVIAG
                     YSAVINWEKAKDDIVIAFIEIRVAPERDRGFEEIAKRIYSYKQVKTCYLMSGSFDIAA
                     IVEGKDLKEVALFVTQKLSTISGVTSTSTHFVLKKYKENELVFEEEEKDQREAIVL"
     gene            complement(23542..23985)
                     /locus_tag="PLQ68_00130"
     CDS             complement(23542..23985)
                     /locus_tag="PLQ68_00130"
                     /inference="COORDINATES: protein motif:HMM:NF016959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HXK71087.1"
                     /translation="MAKKRFRSSMSGYNKDEVNKYIEKMMEEYEAKIAQKDTVINSMQ
                     ETIDDVQKKYEELKGREDTLHKEKDNITKALFKANELSDQIVKEAKESAFKEVTELEI
                     KAEEEREKIVDLKKQLATLQANAAKLLEKFSDSLEKTLGSTEEDK"
     gene            complement(24055..24945)
                     /locus_tag="PLQ68_00135"
     CDS             complement(24055..24945)
                     /locus_tag="PLQ68_00135"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HXK71088.1"
                     /translation="MRKAAILSDIHGNLHMLLKALDICKDQGVERYIFLGDMVTDGPD
                     SLAVLDIISNLTDDVIRGNREEYMISYHKGERDIWDDNHQCESLVKTYENLNDVGLGY
                     ISSLPNVKRISLFGIDTLMVHGSHISTRHFILPSSHIDTFMDMYDRYDCSLFLLGHAH
                     QSYCLKIKDKHFINPGPLGIPCAMKCYNRDDAFSIGIISVDENTKNFDYENVYIQYDP
                     DELSSYYLKDIDKYKHDFWIKLIADSFYTKFYHSLDFLHKAQNMARDGGYENYDPIPN
                     EIWVKAAEKWIKEHKESMIF"
     gene            complement(24946..25848)
                     /locus_tag="PLQ68_00140"
     CDS             complement(24946..25848)
                     /locus_tag="PLQ68_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013781346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK71089.1"
                     /translation="MTVKVEKIPYGGWNNCYRIFNNTVEVVVTSDVGPRIIRYGFIGR
                     FNVMCEKEDQMGKSGGDEWMIYGGHRLWHSPEHEIRSYYPDNFPVDVQIIENGIKVSQ
                     PVEKTTMIKKDIEVVLDDESSKVYLKHTLTNYNLWDVELAAWALTVVAPGGLEVVPQE
                     TLDTLLLPNRMLSLWPYTDMSDKRVQWGSRYIFLQHDSKAIKPFKFGISNTNGWAAYF
                     NNDDMFVKKFSPLEDMVYPDYSASMYETYTCDFMTEMESLSPLMLLGPKEKIEHNEQW
                     ELFSDVKPPKNEEEMDKIARKHIT"
     gene            complement(25931..26293)
                     /locus_tag="PLQ68_00145"
     CDS             complement(25931..26293)
                     /locus_tag="PLQ68_00145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK71090.1"
                     /translation="MDDFENKQENEKKKGYTVDINIDKWYNRRNPNTFPYPVLATIVF
                     LLMGFIWDLWHPGWIVFLTIPVYYAVVNAIKSRAVSYTLIIVLIYLAIGFIWNLWHPG
                     WIIFLFIPIIHWFTGKKG"
     gene            complement(26524..27741)
                     /gene="trpB"
                     /locus_tag="PLQ68_00150"
     CDS             complement(26524..27741)
                     /gene="trpB"
                     /locus_tag="PLQ68_00150"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019879889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HXK71091.1"
                     /translation="MDFTNYTKIYPDIAGRFGQYGGAFLPPELVDAFKEIEQAYMTIA
                     HSASFINELRRIRREFQGRPTPVYHCERLSNRLGRCQIYLKREDLNHTGAHKLNHCMG
                     EGLLAKYMGKKRLIAETGAGQHGVALATAAAFFGLECEIHMGEVDIIKQAPNVTRMKI
                     LGAKVVPVSHGLKTLKEAVDSAFESYMKNYKDSIYCIGSVVGPHPFPMMVRDFQMVVG
                     IEAREQYMEMTGELPDQICACVGGGSNAMGLFTAFLKDPVKIVGVEPMGRGPNVGDNA
                     ASITFGKKGILHGFESLLLQDDKGNPLPVYSIASGLDYPSVGPEHAFLHDIERVDYTS
                     VTDKEAIEAFFMLSRYEGIIPALESSHALAYAIKAARANTANSILVNLSGRGDKDIDY
                     VEKYYGTGEDYKI"
     gene            complement(27763..28803)
                     /locus_tag="PLQ68_00155"
     CDS             complement(27763..28803)
                     /locus_tag="PLQ68_00155"
                     /inference="COORDINATES: protein motif:HMM:NF012804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HXK71092.1"
                     /translation="MYDRFIKEMIEMSCRVGQMPDYTQGGGGNTSVKFDDKLMAVKAS
                     GCKLCDITEKDGFVVVDYAKVRQYYKTVDLTEKRDFYKESSQFVKDNMVKLEGLNEGR
                     PSIEAGFHSLLEKYVIHTHSVYGNLLCCTKEGESIAKELFENSQYRYLWLPYIAPGFY
                     LTLEIQKQIELMGCIPNIIFMGSHGVIITAKTLDEAEKINDYVSQSIRKKLNITELYG
                     DVSVKMESEGVYVSNTPRLIAYLKGKEYTDEWFDSNILYPDQAAYLLGEVTTKNAEKK
                     ARIDLSSGITKYYVSYKEALTLEESICAFLFIMDEAKRNNLETYTMKDEDIAFIMGWE
                     GEAYRKAMLNKK"
     gene            complement(28849..30327)
                     /locus_tag="PLQ68_00160"
     CDS             complement(28849..30327)
                     /locus_tag="PLQ68_00160"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase family protein"
                     /protein_id="HXK71093.1"
                     /translation="MIKVLAYDFGASSGRAMLGTYDGKKINIEEIHRFSNDPVYVHDT
                     LYWDVLRLFYEMKTGLIEAKKAGHEDIQSIGIDTWGVDFGLIDERGRLLENPVHYRDK
                     RTDGLMDEVWDIIGKRNLYERTGIQFMQFNSIYQLYAIAKNNPELLERADKLLFMPDL
                     LSYFLTGKKISEFSIASTTQMLNSKTGDWDFELLSKLKIPTNILEKVEDSATVIGTLT
                     PSVCYETGLKPITVTAVAGHDTGSAMISAPADTDLFAFLSSGTWSILGSELSSPVVND
                     LTYKYEYSNEGGINRTARLIKNIMGLWIYQECRRQWNKEGDPVSFDELEMSARKVKPF
                     ACLIDVDHLDFYHPGEMPKRVVDFCKRTGQYVPQSRAEIVRCIMESLALKYRYVFDGL
                     CEIVGSRIPKLHMVGGGCKNTMISQFTSNALGIEVTSGPIEATAIGNIVAQLIALKEI
                     KNLKEAREIVKNSFEVKSYLPKDLDLWEEKYEYYKKNILNKG"
     gene            complement(30372..31103)
                     /locus_tag="PLQ68_00165"
     CDS             complement(30372..31103)
                     /locus_tag="PLQ68_00165"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HXK71094.1"
                     /translation="MSYEELCRKRDYVNSNLSKLSTEQIEALEKEFECSYTYESTKIK
                     GNRLSRDEVRSILENNAKDMEKDNDVLEITDHSKAFRFMKMYLKKNTYLDEEAVKEIN
                     AIVVEDISVGGVYRNVNLLIPGRKHVPPSPEKAYYKMKKYFNELPYLPYETATEKAMY
                     AYAMFMKIHPFVDGNSRTGKLIMNYVLMKEGFLPIYIPKERYKEYVKAFELFALEDQL
                     EPLSKLAAEFENAQLDKYIEKIDLA"
     gene            complement(31122..32342)
                     /locus_tag="PLQ68_00170"
     CDS             complement(31122..32342)
                     /locus_tag="PLQ68_00170"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HXK71095.1"
                     /translation="MNQYFLLQIENLNNPKSEERLNSLRVLKNAIDKGDIQKPLFFND
                     VNNHIHTNYSFSPYSPTKALFMAYMNGLKTAGIMDHDSAAGCEEFIQAGAILDMPITC
                     GIEVRVRMDKTELNGIRINNPDQDSVAYVALHGIPHQHIKAIQDFMAPYREARNKRNQ
                     MMCNKINDLFGKYDIFIDFEKDVLPLSLYQQGGSVTERHISYALALKMIDLFQKGKAL
                     VSFYKQTLKLPLSEKNENLLSDENNPVYEFDLLGAIKSDLISLFYVDAYDECPDVKDI
                     IALCNKTKAISAYAYLGDVKNSVTGDKKPQKFEDSYIDKLFYILKELGFNAVTYMPSR
                     NTKEQLVRVKALCEQYQLFQISGEDINSPRQNFVCKAMRDSFFDNLRESTYALIKHEQ
                     EATKDITKAMFYNK"
     gene            complement(32345..33121)
                     /gene="srlD"
                     /locus_tag="PLQ68_00175"
     CDS             complement(32345..33121)
                     /gene="srlD"
                     /locus_tag="PLQ68_00175"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HXK71096.1"
                     /translation="MEMLKNKVAVITGAAQGLGEALAQRLDKEGCSVVVADLNYEKAL
                     EVANGLKNAVACKVDVTKEEEVDAMTQLAIDKFGRIDVFVNNAGIVISGSAHDFDVNK
                     WRLVQDVNLWGYFICAKSAVKHMLNNENGASFIQINSKSGKKGSFRNSAYAASKFGGI
                     GLTQSLGLEYAKNNIRFNAICPGNLLNSPLWVNSLFKQYAANQGITEQEVRQKYLDQV
                     PMGRSCEYEDVANVMVFLASEMSSYMTGQAINVTGGQEMR"
     gene            complement(33313..34788)
                     /locus_tag="PLQ68_00180"
     CDS             complement(33313..34788)
                     /locus_tag="PLQ68_00180"
                     /inference="COORDINATES: protein motif:HMM:NF022525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2779 domain-containing protein"
                     /protein_id="HXK71097.1"
                     /translation="MIEFNVSKSRYIRAVQCLKMYWMDRVKPQEFDNSVLDEAVLENG
                     NDVGELALSIFPDISKVAFETDKQIMINQTKQFIDNKSKYIAEASFSYMGRFLSVDIL
                     EIYEDGVVINEVKSSTSVKDVYIDDVAYQSYILNLLGMNVRKANVIILNSEYTRQGNL
                     DLSKLFSINDVTEQAYSRHSKVEELLDFLSQDLDLEKEPEINIAEHCFSPYPCGYFGY
                     CTRHLPEQNIFDIRNLNKKNKLMMYHSGIYSFDDVIYSKEIYAKLRPVIKDQLQEKLV
                     IDKKQILRFLANLYYPIYYLDFETVQYAIPPYDGMKPYQQMPFQYSLHIEKEDGTVEH
                     KEYLAKDGFDCRKELAENLINDIPRQSCSLAYNMAFEKGIIADLAYQYPQHYTALMDI
                     YNNMVDLMVPFKNHWYYDKAMQGSYSIKYVLPALFPEDDAMNYNNLEGVRKGGEAMTA
                     FKNMPLLSEEEKQTVRKQLLEYCKLDTYAMVKIMNKLREIV"
     gene            complement(34785..35348)
                     /locus_tag="PLQ68_00185"
     CDS             complement(34785..35348)
                     /locus_tag="PLQ68_00185"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HXK71098.1"
                     /translation="MRILLIRHGQTDWNINDKVQGSSDIPLNETGIQQAYKTKKILDN
                     YEFDVVISSPLGRAVHTANIVCEDRDNEILIDERLKERDFGKFEGISYRKNMEYRKGA
                     WSLKMEEQLDTVEAYPLFYERISGFLDDILNKYYDKNILLVAHGGVSIAVGHYFKGVP
                     QNGVVHDYILDNCAVAEYDEEIIKAKI"
     gene            complement(35465..37180)
                     /locus_tag="PLQ68_00190"
     CDS             complement(35465..37180)
                     /locus_tag="PLQ68_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HXK71099.1"
                     /translation="MGIDSGLTGADLVWVITGAALVFFMQAGFAIVEAGLTRAKNTGN
                     IIMKNLMDFCLGTIVFWVLGYSLMHGDDIAGIIGLPTTGFITGNIDMTSLIFQTVFCA
                     TAATIVSGAMAERTKFSSYCIYSVIISMIVYPISGHWIWGGGWLSQLGFHDFAGSTAV
                     HMVGGISALIGAKIIGPRIGKYDKDGKPKAIPGHNLTFAALGVFILWFCWFGFNGAST
                     YGVTGQEGILSLIFVTTNMAPAAAAVTCLIITWKKYKKPDVSMTFNGALAGLVAITAG
                     TDTVSPMAALIIGVISGFVVVYGCEFLEKKAKIDDPVGAIAVHGFNGALGTVMVGIFS
                     FNEGIVGAFKGNTSFADAFRFLSIQVLGVAAVALWVAITMTAMFLIIKKTVGLRVSKE
                     EEIGGLDFSEHNLATAYSGFLLNNELDIDKLNVIEYHPSETTPAQMKHVSGKTVNDKL
                     QPKMTLVSIILKKPKFEALKYALNKIGVTGMTVTEVMGCGMQNGHTETYRGAPLDITL
                     VPKLKVDVVISAVPVEKVIEAAKTVLNTGALGDGKIFISDIEDVVKIRTGERGFDALQ
                     DKYCQ"
     gene            complement(37353..38114)
                     /locus_tag="PLQ68_00195"
     CDS             complement(37353..38114)
                     /locus_tag="PLQ68_00195"
                     /inference="COORDINATES: protein motif:HMM:NF012669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HXK71100.1"
                     /translation="MNQRQLQIKQMLDVAQEVFLKQLKTVFPEVSEMTIRRDLILLEE
                     MGYAVRTHGGAVSINRFKKAGEDEIEYMIRQSENVDKKAVIAQKTIHLIEKGRSVYLD
                     AGSTVMCLAKLLTDMKGCTFITSAVNTALKLSNTDNSIIILGGTVNPNTVACSGPEAM
                     QMLETMNIDMAVMGASGFSEDRGFSVSSMYDAKLKTRIVEKARKTIMVVDSSKIDRDK
                     AYTFCELKDIDIWVCDTMPEQKVLDKCSENNVLVI"
     gene            complement(38119..40581)
                     /locus_tag="PLQ68_00200"
     CDS             complement(38119..40581)
                     /locus_tag="PLQ68_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019244842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HXK71101.1"
                     /translation="MTKELVLNPSELRKKSKIKFKDIDVCAYNKTLDEEKKIFTKNDF
                     MRIYRDMAIIREFESMLNAVKTTGQYNSVEHTYPGPAHLSMGQEASVVGEAYTLGIDD
                     FIFGSHRSHGEILAKGLSAIEKLSDKELMDIMENYLDGRILKVVQKDQKTVKELAMDF
                     LLYGTLAEIFSRETGFHRGLGGSMHAFFIPFGIFPNNAIVGGSADIAAGAALFKKANQ
                     KKGMVIANLGDASMGCGPVWEGICFASMDQYRTLWEKEHKGGLPIIFNFFNNCYGMGG
                     QTKGETMGFDLLARFGAGVNPDQMHAERIDGYNPLAVIDAYKRKREIIEKKQGPVLLD
                     VLTYRFAGHSPSDASAYRTKEEIDMWMEQDSLIEYRQKLTKGGIAKDEEFDSILSDTA
                     SLIFRIFKMAIDETITPKMDLVANPRAIEELMFSNRRIEKMEDRPCDAYAPLEENARY
                     QQVQKKERMGIVDGKPVSKNKVYQIRDAIFEAMIEKFYTDPTLIAYGEENREWGGAFA
                     AYRGLSDSLPYHRLFNSSISEAAIVGSAVGYAMAGGRAVVELMYCDFLGRAGDEVFNQ
                     LPKWQSMSAGILNMPVIVRVSVGAKYGAQHSQDWTSLCAHIPGLKVVFPVTPYDTKGL
                     LNAALNGTDPVIFFESQKLYDKGEMFHEGGVPTEYYEIPIGDPDIKRVGKDVTILTVG
                     ATLYTALEAADELKEKYGMEAEVIDARTVSPFNYELVAESVKKTGRIVLASDACERGS
                     HLKDMAATISELCFDYLDAPAVVVGSKNWITPAADYEKFFFPQPSWIIDAIHQKIVPL
                     KGHTASVNYTEGETIRINKLGV"
     gene            complement(40632..41999)
                     /gene="lpdA"
                     /locus_tag="PLQ68_00205"
     CDS             complement(40632..41999)
                     /gene="lpdA"
                     /locus_tag="PLQ68_00205"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015907260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HXK71102.1"
                     /translation="MKYDLIVIGGGPAGYLASERAAAKGLNVLLFEKRNLGGVCLNEG
                     CVPSKALLYSAKIYDYTKHSEQYGIKVTESSIDQQVVIKRKNKVVKTLVTGVKAALKH
                     HNVTVVEKTAKIEKKSSEGYVVSADNEKYLANRLLICAGSVPIMPPIPGLHEGYEKGF
                     VLTNREVLDLTEIPENLVIVGGGVIGLEMASYYNSVGSTVTVVEMLDKIGGPIDDEIS
                     ELLRKEYEKKGVIFNLGSKVTSFTENSVIFEKDGKSYEILADKTLVSIGRRPATKDLG
                     LENIGVETDKRGAIITDSFGRTNVAEVYAAGDINGISMLAHTAYREAEVCVNNMTGKK
                     DIMRYNAIPSVIYTNPEVASVGETEATAKQKNMNFESVKLSMKFSGRYVAENEGGNGI
                     CKVLREKDTNRLIGVSLIGNYASELIYGAAMMIETQMTVEDIKELVFPHPTVCEIIRE
                     ALFAF"
     gene            complement(42013..43356)
                     /locus_tag="PLQ68_00210"
     CDS             complement(42013..43356)
                     /locus_tag="PLQ68_00210"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HXK71103.1"
                     /translation="MATAVIMPRQGNTVESCVVSKWHKKKGDKVNVGDILFTYETDKA
                     TFDEESKVEGVLLETYFEEGDDVPCLLNMCVIGEEGEDISGFTLDGAQESKPESQENK
                     DTKTVEDSKPERKKEPIKANDSESLKISPRAKSLADKTGVDVLYAEPTGPNNRVIERD
                     VQKLAKDGTKVTSAAKSGYVEGASYNATGIGGRVTVDDLSKQVQPEHMLAKTEEGFVE
                     YTEEKLTNIRKVIARTMHSSISEMAQLTMNSTFDATGILNYRAMVKANAERLGLANIT
                     INDIVVYAAAKTLLSHKDLNAHFANDVMKRFNTVHMGVAIDTPRGLMVPTVFNADMKS
                     LNDISLQVKELAAKSKSGSISPDLLSGGTFTITNLGNLGVESFTPVINPPQTAILGVC
                     GLTTRVKEVNGQLKAYQAMGLSLTIDHRVVDGAPAARFLQELCSNLENFEMMLAK"
     gene            complement(43458..44876)
                     /gene="gatB"
                     /locus_tag="PLQ68_00215"
     CDS             complement(43458..44876)
                     /gene="gatB"
                     /locus_tag="PLQ68_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003530973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HXK71104.1"
                     /translation="MTSYKGYIMTIGLEVHVELKTDSKIFCSCSTDFGASANTQTCPI
                     CLGMPGTLPVMNIKVVEYAIMAGLATDCKISLNSKMDRKNYFYPDLPKAYQISQYDMP
                     LCINGYLDIVTAEGEKRINITRIHLEEDAGKLIHDDEKGTMIDCNRCGVPLIEIVTEP
                     DFESTEQVGIFLNKLRTILLNLGISDCRMNEGSFRADVNLSVRKETDKLLGTRTEMKN
                     LNSFAFIQKAIEYEFVRQVDSLLADKKIVQETRRYDEKNKKTYSMREKEDADDYRYFP
                     DPDLVPINLTDEYVQSIKRILPILAKDKKPQIMKKYGLSSFDSEMLVSDMKISVLYET
                     AASLTSAYKILANLMIPLSEKALEIKAERWAVIADMTDKDQINTTTAKKLIDMLLKED
                     FDPKQQVKIKNMFQINDIQKLKEYVTKAISLNTKSVESFKSGNIQAMGPIVGYVMKIT
                     KGCANPSKVNEIVLDMLKDMDI"
     gene            complement(44869..46323)
                     /gene="gatA"
                     /locus_tag="PLQ68_00220"
     CDS             complement(44869..46323)
                     /gene="gatA"
                     /locus_tag="PLQ68_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009621246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HXK71105.1"
                     /translation="MKDVLKKDILSLHELIIKKELSCVEITKAYLDNISDKETDIQAY
                     ITITEDLALADAKKKDEQLTSTDNVSLLYGIPGALKDNICTKNIKTTCASKMLEDFIP
                     PYDATVYDKLKKDGMVLLGKLNMDEFAMGSTTTTSHFHKTHNPYDLSKSPGGSSGGSA
                     GAVAASQTVFSLGSDTGGSIRQPASFCNVVGLKPTYGSVSRYGLVAFASSLDQIGPIT
                     KNVYDNALVFDAIKGHDVLDSTSVKRLYDTSTCCIEKDISKKRMGIIKQFCSQGIDDS
                     VSIRIDKAIKWYASQNIEIVELDMPSLDWAIYAYYIISSAEASSNLARYDGIRYGFRA
                     DEYKDISSLYEQSRSLGFGSEAKRRIMLGTFVLSQGYYDQYYNKAQKVRSLVSMQFDE
                     AFKKCDFILAPVTPSTAFSLDNIEKDAVKMYMEDIFTVPVNIAGLPALSVPCGMTDEK
                     LPVGMQLIGPRFSESMLYQLGYAYEQNNKENRND"
     gene            complement(46325..48394)
                     /gene="aspS"
                     /locus_tag="PLQ68_00225"
     CDS             complement(46325..48394)
                     /gene="aspS"
                     /locus_tag="PLQ68_00225"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010168124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HXK71106.1"
                     /translation="MKRTQYCGLISEDYVGKSITVNGWVWSKRDMGGVIFIDLKDREG
                     ILQTVFDLNDVTDDVFSLAEQIKLQSVVQMHGIIRIRGEETYNDKLKTGTIELKVSDI
                     VVLSEASNLPFAIDDNTFIKEDLRLKYRYLDIRRTEISDNLRFRHMVSKVIREYLDDD
                     GFVEIETPILTKSTPEGARDYLVPSRVHQQKFYALPQSPQIFKQLLMVGGMDKYYQIA
                     RCFRDEDLRADRQPEFTQVDMEMSFVEQEDVLIHLEKLFKHIFKKSIGKEISDPFLRM
                     TYTEAMDTYGCDKPDLRYGLKIHDVTEIVKKSGFSIFKKSECIRAINYKNKASMTRGE
                     IEQLTKKALEFGAKGMAWIAIESDGTINSILTKYIEKKEMDELFTQMDAKPNDFIVFC
                     ADTFENSRKILGSLRIHIAQNDNMCDRQAYSFVIVTDFPLFEYSEEQKRFVAAHHPFT
                     CPHPDDLKYMLTDKKRIRALSYDIVLNGTELGSGSIRIHDSNMQNLMFEALGMNKEQI
                     KSQFGFLVKAFSFGTPPHGGFAFGLDRLIMILRQASSLRDVIAFPKIKDGSCVMTKAP
                     DYVENEQLKALHISYKDHFEMKEKRKNDIEIPIDKLSKLARLSLREEEKKSLKEDMQN
                     IIKFASNIMDIDTSNVEQKYTCFEMENVFREDKALKSLSEDELLSNAKTHSDGYITVP
                     AVLKAEV"
     misc_binding    complement(48449..48692)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(48814..49458)
                     /locus_tag="PLQ68_00230"
     CDS             complement(48814..49458)
                     /locus_tag="PLQ68_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015521138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HXK71107.1"
                     /translation="MKSVLIIGTGKFGKNLALKMAELGNEVMVVDKEENEVRDISEYV
                     TSAQVADCMDENVLRSLGINNFDICFVCIGDNFQSSLEITSLLKDNGAKYVVSKADRD
                     IHAKFLLRNGADEVIFPEKDMAQRAAYRFSANHMFDYIQLSQDYSICEIETPENWVGK
                     TIAQVNVRSNYNVNVLAYKENGKIYPLSNPNYIFKENTHLIISGAWKDVEKLNK"
     gene            complement(49469..50812)
                     /locus_tag="PLQ68_00235"
     CDS             complement(49469..50812)
                     /locus_tag="PLQ68_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015379 - potassium:chloride symporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HXK71108.1"
                     /translation="MELKKTKKISTMQIIAVGFVVIILIGTFLLSLPVASRNGIRIPF
                     IDALFTATSSLCVTGLVVYDTYTQFTLFGQLVILTLIQIGGLGFMSMATMISLLIGKK
                     IGLKERGLLMETMNTNQIGGVVKLLRKIIVITFSIEIVSAAILSIRFIPLLGTAKGIY
                     YGLFHSISAFCNAGFDLMGVFEARSSLITFNTDVLVNIVVMSLIVIGGLGFIVWDDLM
                     NNKFKFSKYRLHTKIVLIGTAVLILIPAVIFFFLENSNTLEGMTAGQKIMASVFQVIT
                     PRTAGFNTVDTASLGDGTTFMTMLLMIIGANPGSTGGGIKVTTLFVIILTVKAYVTRS
                     QDLNAGGRRLEEYVPRKAFSITTLYLLLSLFGAFLIMSTQQLGMKETVFEALSAIGTV
                     GLSRGITPDLSTISKTVIILLMYCGRVGSLTVLSAFAGDRIKPNIRSIEEKIVIG"
     gene            complement(50817..51881)
                     /locus_tag="PLQ68_00240"
     CDS             complement(50817..51881)
                     /locus_tag="PLQ68_00240"
                     /inference="COORDINATES: protein motif:HMM:NF025957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HXK71109.1"
                     /translation="MKKAISYYEGLKPHTNLDDEDLIYLDIRKKPFEIYGLYDPLNQP
                     VFKRLPDEVAAKCNSGVQKTYLHTSGGRIRFSTDSPYLCIQVFTKEINRMNHMPLLGS
                     SGFDIYQEINGKSVFIDKFVPCVDTKDWYEMRIRFVNEGLKNLTIYFPLYNQVDSVYI
                     GVGKESLIGEGIRYTHEKPVVYYGSSITQGGCASRTGNSYEAIISRMCDTDYMCLGFS
                     GSALGESDMAEYIAKMDMSMFVMDYDYNAPTPEHLEKTHEEFFKKIRNKQPGLPVVFV
                     TKPNFSDYSFDNIQRRNIIYKTYMNAMQKGDRNVYYIDGRSLFQDDYRDCCTVDGVHP
                     NDFGFVRMAQAIGRMVKELL"
     gene            complement(51900..53303)
                     /gene="purF"
                     /locus_tag="PLQ68_00245"
     CDS             complement(51900..53303)
                     /gene="purF"
                     /locus_tag="PLQ68_00245"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009986976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HXK71110.1"
                     /translation="MDKIKEECAIMGVSLKCEEAVGAVYNGLLALQHRGQEGAGIALS
                     HNQSIIIQKNTGLVNEVLTEKVLDKLPKSRTVIGHARYSTTGSNTNKNVQPFLTEYLT
                     GRIATAHNGNITNAKQIKVMLEKQGLDFSASSDSEVISSLIAYNIISNRDLLKGVIEA
                     AKLLKGAFSLVLLTGDKRLVVVRDPHGFRPLCIGKNEFGIAAASESCALDSCGFEFIR
                     DVMPGEVIIIENGKIQYSNVELTANKQGLCIFEYIYFARPDSIIDGLSVYEARFNMGR
                     MLAKEHPIDADVVCGVPDSGLEAAMGYAYESGLPLVSGFVKNRYIGRSFIYPSQLQRE
                     HAVKIKLNPLKANVQGKSIILVDDSIVRGTTSAKIISSLRSTGVKEVHMRISSPPFIH
                     TCHFGTDIDNEKNLIANQITNQQICERIGADSLGFISVEGLKKACEKCTLPFCTGCFN
                     NDYGTDSVEISKYQLES"
     gene            complement(53339..55345)
                     /locus_tag="PLQ68_00250"
     CDS             complement(53339..55345)
                     /locus_tag="PLQ68_00250"
                     /inference="COORDINATES: protein motif:HMM:NF017215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HXK71111.1"
                     /translation="MALGIGIDTGGTYTDAVIYDFSSESVVCSSKARTTHDFLEEGII
                     NALNQLDQSYFDKIEIVALSTTLATNACVENRGGRAKLMFFGVDKKVFDMVGKNYGLV
                     NKDEVYLCDNASDFNGSKAEEPDWDKLFDNLADWLKDAQGLGIVEVFAMNNGAMLEKK
                     AKEQVSKRYDIPVVCGHELFYELNSIQRGSSTLLNAKLIPIIQEFVESIKKALEEKGI
                     FAPIAIIRSDSTKMSEQYSMTKPVETILSGPASSVTGGINCTKETDCLIVDMGGTTTD
                     ISIVKNGVPLNADSGVRIGSWKTFVHGVFIDTFGLGGDSGIRVEHGRPVLLSQRRIPF
                     CVASKKWPCIKEQLNELIDNGRYHSHPLYEFLVLQKDIKNTLIYSDDEIELCNILKKE
                     PILIEKAALAVGRDVYTINTKRLETEGIIIRIGLTPTDIMHLKGDFTQFDTTAPKLAL
                     EYILNCILEETGLEIEQEQFCDMVYESVKLKLYENIVRILLCDKYPDIFKNKMDEQIT
                     SLIQNSFKDKEQNNIPALFKTDFSLVGIGAPTHIFLPDVAKALKTRCVIPENAKVANA
                     IGAVVSNIRSAHQIEIRAEYDGSYLIFGPEGKVKESDISKALLYAEEKAKEVALKDAK
                     SKGLSDNYNIDTQVITKTATAKKGREIDLGTVVIATISGKIEYN"
     gene            complement(55412..56668)
                     /locus_tag="PLQ68_00255"
     CDS             complement(55412..56668)
                     /locus_tag="PLQ68_00255"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HXK71112.1"
                     /translation="MITIAVIGLGNRGSGYMRWTRLFNGNKARITALCDSDEYRLNSY
                     GDKYKIDKDKRYLDEHKFFKAGKLADAIYICTMDKDHFRHAMAAIKCGYHIMLEKPVS
                     TNIPECEMIRDKAKEKGLHVVVCHVMRYSPFFKKMNQIVKSEKYGKIISIEHTENIGY
                     FHFAHSYVRGNWRDSETTTPMLMAKCCHDFDLIHWLMGDGCQSISSYGSLSYFKKDNA
                     PKDSAKICLECPRHVKSKCPYDAEKLYITAPIWRFTFLKFLGNVITGKPKYNRKDKYE
                     SLRTGLYGRCVYRCDNNACDHQVVTMDYKNDRYVNLNVTAFSRLNYRRTSIHMEKADI
                     LCDESKGRFIINEFGKSKKVVRAGVIHFVHISGDIAIVKNFIKLLNNEPIKKDDLTFI
                     DTTIVSHRNVMLAEQSRKEGGKLIYL"
     gene            complement(56665..57015)
                     /locus_tag="PLQ68_00260"
     CDS             complement(56665..57015)
                     /locus_tag="PLQ68_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015540292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
                     /protein_id="HXK71113.1"
                     /translation="MNIQIFGKSKCFDSKKAERFFKERKISFQYVDMAEKGLSKGELE
                     KVIRATGGIDNLVDEKSKDKDKLLLFKYAKNDNKFDILLLNQQIIKTPVVRNGNQATI
                     GYEFETWKKWEAEK"
     gene            complement(57777..58610)
                     /locus_tag="PLQ68_00265"
     CDS             complement(57777..58610)
                     /locus_tag="PLQ68_00265"
                     /inference="COORDINATES: protein motif:HMM:TIGR01856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase"
                     /protein_id="HXK71114.1"
                     /translation="MNKLSTIHLHTNYSDGVNKVYEYIEEAIRLRLKSIGISDHSPVP
                     FNSTWAMKKEDLSKYIAELDYLKRTYKKHISVYAGMELDYIQGYNIKKYIDFDNLNLD
                     YFIGSVHYIYSEITDSYGTADNSQEEVEKLISNGFNNNGVLFYKKYYQTVQEMIKEYD
                     PIMIAHIDVLEKRNRNYELFNPDTEEYKNQIENTLNCINKSCVEVNTGAMAKNIDVMY
                     PSKYILKQCFKKKIPVSVNSDCHFISGLQYGYDKAKDTLLDIGYTHIFEYDGKFIKKE
                     L"
     gene            complement(58603..59538)
                     /locus_tag="PLQ68_00270"
     CDS             complement(58603..59538)
                     /locus_tag="PLQ68_00270"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HXK71115.1"
                     /translation="MKKMYSLVIPLYNEEEVIHECIKRVTNVMHNNEFDYEIIFVNDG
                     SSDSTEEIVKQYCNKDKHLKLISFSRNFGHQTAITAGMDNAEGDAVIVMDADLQDPPE
                     VVLKLIEKYEEGYDVVYAIRSKRKGESFFKKISAKMFYRFLKNMCEVDIPVDTGDFRL
                     ISRQVCDVLKGLTERNRYVRGLVSWVGFKQTGIYYEREERYAGKTKYPLKKMLKLSVD
                     GITSFSTKPLKLTKWIGIFMAAVGFIYAIIVIIQKLVGIQMQTGWASTMVTILLIGGI
                     QLIMLGITGEYIARIFDESKNRPLYIIKEKVNMDE"
     gene            59767..60147
                     /locus_tag="PLQ68_00275"
     CDS             59767..60147
                     /locus_tag="PLQ68_00275"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HXK71116.1"
                     /translation="MDSLYRKYGRRVREKRVKINMSQETLAEKCQVSTSYIGLVERGE
                     RKPSLLVLVRIANALNVSTDSLLFDSLRHIASSNKDRIMMMVNDFDDPQIDTTFEIVN
                     QIHSYLKKDVPKIDRVDIDQILND"
     gene            complement(60144..60338)
                     /locus_tag="PLQ68_00280"
     CDS             complement(60144..60338)
                     /locus_tag="PLQ68_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010630834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HXK71117.1"
                     /translation="MRPDVQINDIVELKKKHPCGSVKWRVLRVGADFRLECLGCGHKI
                     MLDRNVFFRKLKNLINSEES"
     gene            complement(60335..60496)
                     /locus_tag="PLQ68_00285"
     CDS             complement(60335..60496)
                     /locus_tag="PLQ68_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HXK71118.1"
                     /translation="MDRYICTVCGYIYDPDNGDEIGCIPEGTEFNDLPIDWICPECGA
                     EKELFEIEE"
     gene            complement(60564..61202)
                     /locus_tag="PLQ68_00290"
     CDS             complement(60564..61202)
                     /locus_tag="PLQ68_00290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK71119.1"
                     /translation="MRRTIVLILIIALSISMLAGCDSKSTEIVKPLEIGRGEVVDGIY
                     TNKDMGISIEVPEGYHIATEEELETSFDITHLLEDEDMVSYITYLAFFSLKPIDDAEN
                     NSHIMISVEDASKYGYSDKDTYIEYMAYEKSSAYQSIEGAEVTVSNHKNVWLSNRKFA
                     YRSITLDMNDLYMVFDMYGVIKNGYFLTIMIGAFSNEDLDFLRGYLDTFVIE"
     gene            complement(61205..61621)
                     /locus_tag="PLQ68_00295"
     CDS             complement(61205..61621)
                     /locus_tag="PLQ68_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019907754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HXK71120.1"
                     /translation="MKSFRKELYFNIKTRRGFVNITPQLQQCLYESGIKEGLLLCNAM
                     HITASVFINDDESGLHSDMEKWLEKLAPEKPYSQYAHNGFEDNADAHLKRTVMGREVV
                     IAITEGKMDFGPWEQVFYGEFDGLRQKKVLVKIIGE"
     gene            complement(61683..61991)
                     /locus_tag="PLQ68_00300"
     CDS             complement(61683..61991)
                     /locus_tag="PLQ68_00300"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXK71121.1"
                     /translation="MTDVIILKIDYQVNEGQVLEFFKECKVISTPGHTPGHISIYVKD
                     IDAVIAGDAVVIENRKLKIANPSFCFDIDKAEKSLKKITNMNVKRIICYHGGEYIMQE
                     "
     gene            62255..62524
                     /locus_tag="PLQ68_00305"
     CDS             62255..62524
                     /locus_tag="PLQ68_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014759623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HXK71122.1"
                     /translation="MNKREFIKAMSAESGLTVKDTDKAYDAFLEVVAKALKEGDKVQL
                     AGFGTFEVKKRAARTGVNPKTGEKVQIKASKAPALRFGKSFKELF"
     gene            complement(62721..62796)
                     /locus_tag="PLQ68_00310"
     tRNA            complement(62721..62796)
                     /locus_tag="PLQ68_00310"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(62761..62763),aa:Thr,seq:ggt)
     gene            complement(62830..63426)
                     /locus_tag="PLQ68_00315"
     CDS             complement(62830..63426)
                     /locus_tag="PLQ68_00315"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HXK71123.1"
                     /translation="MTDNSLEQLINKAKNGDVIAMDVLLKKYSYLVRKRANGLFLIGA
                     DKDDLLQEGMIGLFKAVISYNEEKDASFDTFASLCINRQMISAIRGANRYKHKLLNEY
                     ISIYSDENQQETQYAVSIPDNKFNPENIITQEETIEHILSKISSRLSKMESDVFDLLI
                     EGQTITFMCKTLEKDAKSIDNAIQRVKNKVRNILKEEH"
     gene            complement(63416..63910)
                     /locus_tag="PLQ68_00320"
     CDS             complement(63416..63910)
                     /locus_tag="PLQ68_00320"
                     /inference="COORDINATES: protein motif:HMM:NF017774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HXK71124.1"
                     /translation="MEYLLVDGYNIINAWRDIFDKENPLEDNRKMLCELLSDYQGYTD
                     LRIVIVFDAHLVPEGKGSLINHDKIKVIFTMSNQTADNFIERYVTQQRYADKITVVTN
                     DYLEQKTVFQKGALRMTANELRSEIFTKRKNGIANTNNSNFIIDNLDNEQKKKLLRMR
                     NDDR"
     gene            complement(63917..64660)
                     /gene="rlmB"
                     /locus_tag="PLQ68_00325"
     CDS             complement(63917..64660)
                     /gene="rlmB"
                     /locus_tag="PLQ68_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HXK71125.1"
                     /translation="MEENTSVIAGRNPVLEAIKKEYLISRILVSSGEKEGSILRILSE
                     AKKRNIPVKQVSSSKLDSIMSSHQGVVAYISPIEFIEVEDILDLAKEKNEPPFIVILD
                     QIEDVHNLGAIARSAEAAGCHGIIIPLHRSAPVTAAAVKASAGALLHIKVARVTNLNS
                     TIELLKKEGLWICGTHQNADYEYNKADLKGPLAIVIGSEGEGMSHLVAQNCDFTVNIP
                     LVGNITSLNASCAAAIVIFEALRQRRQGS"
     gene            complement(64660..65052)
                     /locus_tag="PLQ68_00330"
     CDS             complement(64660..65052)
                     /locus_tag="PLQ68_00330"
                     /inference="COORDINATES: protein motif:HMM:NF012843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III domain-containing protein"
                     /protein_id="HXK71126.1"
                     /translation="MFFKDLHVNDILDMPVLSLAYIGDAVYDLYVREHLIVKGCYKPI
                     DLHKMTVSLVNAKSQKQSLLKIMDILNAKELNCVQRGRNAKTKSKPKNADATDYTYAT
                     ALEVLIGYLYVNKEYERLDYLLGVMLEI"
     gene            complement(65052..66053)
                     /locus_tag="PLQ68_00335"
     CDS             complement(65052..66053)
                     /locus_tag="PLQ68_00335"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017118 - lipoyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HXK71127.1"
                     /translation="MKNLIVINKSHNPWYNLSLEEYLLDKITEYDNILFLWQNANTVV
                     IGKNQNPWRECRTELLESEDGKLARRSTGGGAVYHDLGNLNFSFILERSSQDFNLQAR
                     IIIQAVKKFGIEAYVNGRNDIVTEEGKFSGNAFTFRRNRALHHGTVLLDVDREKLGRY
                     LQVSKEKMEAKGIKSVQSRIVNLREYNNDITVDDLKFSIIEAFEKEFGTCEIVNEFDN
                     PQSLKKFVNKEEIDRIYDIYSSWDYRYGQAPRFDIEWVHRFSWGGIEIHLCLKNGIIT
                     QSRIFSDALDEPFITSIQDCFNNVRFKKQDMIKAISAKKSDSPMAHDIIEYIQSKEF"
     gene            complement(66050..66670)
                     /gene="plsY"
                     /locus_tag="PLQ68_00340"
     CDS             complement(66050..66670)
                     /gene="plsY"
                     /locus_tag="PLQ68_00340"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004630169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HXK71128.1"
                     /translation="MTVFLYIILAISGYLIGSMNSAILVSKLYKKDIRGEGSKNAGLT
                     NTLRVLGTRAAVMVLIADILKGVIATGLGYIVIKNGLSGAICAGIFTIVGHIFPVFFN
                     FKGGKGILISLSVLLMTDWRIALTGLGVFIVIVVLTRFVSLGSMIAVFLAPFLAWVFN
                     HEIYFILAMAFLATLTIFMHRKNIKRLLSGTENKLSFKKKDKGGEK"
     gene            complement(66667..67992)
                     /gene="der"
                     /locus_tag="PLQ68_00345"
     CDS             complement(66667..67992)
                     /gene="der"
                     /locus_tag="PLQ68_00345"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014314066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HXK71129.1"
                     /translation="MTKPIVAIVGRPNVGKSAFFNYLAGKRISIVQDTPGVTRDRVYI
                     KCDWRGREFILIDTGGIETKASDEIFKHMHRQAEVAIDTADVIILMTDITTGLTAGDR
                     DVANLLHKSGKPVVIAVNKVDRPGDPPAELYEFYNLGLAEVFPVSALHGLGIGELLDE
                     VFVHFPEEPDEYEEHDGINVAIVGKPNSGKSSLLNRIVGEERAIVSPVAGTTRDAIDS
                     LIEKDGKKYTFIDTAGIRKSSKIFSDIEKYSIIRAWSAVERADVCIIMIDSIEGISEQ
                     DSKIAGFAHEQGKAIIVALNKWDLVEKDEKTFLSYVQSVRKELPFMQYAPVVSLSSLT
                     GKRVDNLFPMIDKAYENSCRRIPTGVINDVLNDAIAMVQPPSDKGKLLRIYYMTQVST
                     QPPTFILFVNDKNLMHFSYERYIANTLRDTFDFEGTPIRFIIRERGKDN"
     gene            complement(68079..>68679)
                     /locus_tag="PLQ68_00350"
     CDS             complement(68079..>68679)
                     /locus_tag="PLQ68_00350"
                     /inference="COORDINATES: protein motif:HMM:NF016354.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF512 domain-containing protein"
                     /protein_id="HXK71130.1"
                     /translation="DIIKQIEGYQKKFKEKAGYNLIYAADEFYIKAGLDIPDFCEYDG
                     FYQIENGVGMMREFIYDAKEYLDQLSLNKKELKRLKKKKKHINVATSVLSFSFIEQLV
                     KEVMKYAPDVKIDVYKLENNFFGGHVTVTGLLTGQDLLDGLKKVDLKDGLLISRTMLK
                     ADEDIFLDNYSLKQISKELKTTITVVNNNGAQFIEALVK"
     assembly_gap    68680..68779
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<68780..69265)
                     /locus_tag="PLQ68_00355"
     CDS             complement(<68780..69265)
                     /locus_tag="PLQ68_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004622626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HXK71131.1"
                     /translation="MEKKAIAIKQVQNRSLAKKAGIKSGDFLLSVNDVSIKDIFDYQY
                     QISNEYITLEVIKQKTGKLVKLEIEKDIDDDLGLVFEKELIDDEKSCHNKCIFCFIDQ
                     LPKGMRESLYFKDDDARLSFLTGSYVTLTNMSYDDISRIASMHFSPVNVSVHTTDPKL
                     RC"
     gene            complement(69302..69487)
                     /gene="rpmF"
                     /locus_tag="PLQ68_00360"
     CDS             complement(69302..69487)
                     /gene="rpmF"
                     /locus_tag="PLQ68_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007934595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HXK71132.1"
                     /translation="MAVPKSKVSKQRKRKRRTFYKLETPNLVRCSQCHTLKRQHRVCP
                     ECGYYDGKEVIKQEAEK"
     gene            complement(69512..70012)
                     /locus_tag="PLQ68_00365"
     CDS             complement(69512..70012)
                     /locus_tag="PLQ68_00365"
                     /inference="COORDINATES: protein motif:HMM:NF014657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HXK71133.1"
                     /translation="MKIDITDIIERDGTAKKFEMDVDSHKTGGGMTLVGFLGDIHLKG
                     DICNNEGILTVTAEITGQLDAKCYRCLKQIVKPFTIEMEEIFEPANSSDNLDNYEYSG
                     RILDISQAVIDSIFLNIPDRVLCVPDCKGLCPNCGCDLNEKECKCKEESQENNKFAAL
                     RDLIKE"
     gene            complement(70085..71287)
                     /locus_tag="PLQ68_00370"
     CDS             complement(70085..71287)
                     /locus_tag="PLQ68_00370"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013297978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HXK71134.1"
                     /translation="MKVLVVNTGSSSLKYQLIDSSNERMLAKGLCERIGLVNGIHSYT
                     NSENKSFVNECVIRDHAYAFKIMLEALISDEYGVLKDISEIDAVGHRVVHGGEKFNKP
                     VLIDDSVIKIIEDCIDLAPLHNPAGISGIRVCQEILKDVPMVAVFDTAFHQTIPDYAY
                     MYALPYEMYEKYGVRKYGFHGTNHRYVSRRAAEIMQKPLEKIKIVSCHLGSGASICAI
                     DKGMSIDTSMGFTPLAGLAMGTRSGTIDPSIISYIMDKKHMSVEEVNDYLNKNSGILG
                     VSGISSDFRDLIKAKDEGHKRAQLALNIFAYRVKKYICEYVGVMGGIDALIFTAGVGE
                     NNAYIREAITNNLDIFGIKMDKKKNNAAKGEADVSKDRSNVRILIIPANEELEIARQC
                     KSLLEAVN"
     gene            complement(71314..72306)
                     /gene="pta"
                     /locus_tag="PLQ68_00375"
     CDS             complement(71314..72306)
                     /gene="pta"
                     /locus_tag="PLQ68_00375"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014314070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HXK71135.1"
                     /translation="MTFIERTIQRAKTNIKTIVLPESEDIRTLKAASTVLKEGIADII
                     LLGNKEQIMKDAKGLYIPEDKIVEIDKFEKMNEYANALYQLRKEKGVTLEIAYKLLEN
                     PLYFAVMMVKMGDADGMVAGAVNATSNTLRPALQILKTAKGTKIVSAFFIMCVPDCEY
                     GANGVFAYGDCGLVENPDADQLSEIAISTSKSFEMLVEEKPVVAMLSYSTYGSAKSEL
                     TDKVIEATKLAKEKAPGLLLDGELQVDAAVVKEIGQKKAPGSEVAGKANVMIFPNLDA
                     GNISYKITQRLAKAEAYGPLLQGIAKPVNDLSRGCSAEDIVGVIAITAVQAQFA"
     gene            complement(72442..73665)
                     /locus_tag="PLQ68_00380"
     CDS             complement(72442..73665)
                     /locus_tag="PLQ68_00380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK71136.1"
                     /translation="MAVTKGIKENTAKKDSKKNTKEISKEETIIRYGFLGLGVIVALC
                     IIYFVVASLIPKNYVKVGNSNIAEEEFMYKTNTQYNVFVNYYQDYIAGLGFDITTQDG
                     VLNFLKTAFDNQRSFGQMLLENVVDSIQEQYIILDLIEETGFQIDEQELEESYNIQLT
                     NIQKAADEAGYDLDDYAKIYYGTTFKTMEKVIKQTLKVQQYSSYIEEQNLSAVTEDRA
                     RAYYESVDGEGNKIKDDIDKVTVVTVLKRTVDEQLNPLSDDVVAAAKIKAEEVYQLAL
                     GGQDIYELAELYSDDLVDSVEDEEADQEEETSTTKPGEYTFTKNEVRVTDLADWAFEA
                     TEGDITLLETEIGYMVIKLIDRTEFDTVKDDVYRLISSSDYELQMQEYKAMSKYAVMH
                     YRLYQDLYLSYQTEQ"
     gene            complement(73751..77206)
                     /gene="mfd"
                     /locus_tag="PLQ68_00385"
     CDS             complement(73751..77206)
                     /gene="mfd"
                     /locus_tag="PLQ68_00385"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HXK71137.1"
                     /translation="MNPFVQVFSEIPQQKGIADCIVMKQTPVGLSGPGNSLCPLVSYV
                     TAKMVSRKLLIITHSRTSAISIYQNLISLYGDDVVYLPVRELMLFDIDAYSSNEKQAR
                     INALFRIATQDFQAVVACTEALTVGTTSKEIFINHLIQLKKGYEYDMQALCDKLSELG
                     YSRRALTEEQGQFSVRGGIVDIYSPNYDNPVRCEFFGDEIDSLRFFDAQTQRSIDDVG
                     ETTIIPFCEFLITRDEEDQISEKITDNWDLQKLQNGEYSKIMDRYFAILGKCVMTAEY
                     FKDMNVLTVVHNDVRCHAVAETVYKEYANACVNLLQKKKTLTQACVMQKDIYSLYSFL
                     KPDLVLVYDKSDYNIPLYRDFDISFDQPASYRGNMELFLEDFYEYKNQDMYIFISSDR
                     RKQTEYLKGFFEDNGFSTHIIDKGQEYAIQPKSVYILEDGFNESFVSKEAKIVSISVS
                     RIFRKNAKQKPKKIRDDDFFAQIQPNDYVVHDYHGIGIYQGVEKIEIEGIIKDYIKIA
                     YRNEDVLYVPVSKMDLIQKYVGSGEKTPRITGLGGKEWEKQKNKVRKAVRDIAKQLIE
                     LYSRRTAVKGHIFLQDTPWQISFENEFEYEETDDQTRCVEEIKEDMESAKVMDRLLCG
                     DVGYGKTEVALRAVFKAVMDGKQVAFLAPTTMLALQHYNNFVERMKNYPVNIDLLCRL
                     RNKKRQSKTISDLLKGKVDILVGTHRLLEKDVIFKDLGLLVVDEEQRFGVAQKEKIKT
                     LKPTIDVLSLTATPIPRTMHMALSGIRDVSTIYDPPEKRFPVQTYVLEYDPEMIKEAI
                     LREKDRGGQVFYLYNRVMDMDNKFYQLQNMLKDDVRICIAHGQMQEKQFEDTIIRFIN
                     HEYDLLLCTTIIEAGIDIPNANTLIIEDADRLGLAQLYQIKGRVGRSERVAYAYITYK
                     RGKELTEEASKRLQTIKEYTEFGSGIKIAMKDLEIRGGGDLLGANQSGFMQGVGYDMY
                     VRILSEEIARMKGEKVKDALVETSIDLNISAYIDKSYIQDDLSRLKMYKRIALVESVS
                     EKQDIIDELIDRYSEPPGHVLNIIDVAYTKNLASKAGISEIKQNKKGFDIIYDKKEAF
                     RFGDVINVLDVLKCRYSVKTYDFLRIRVETNKKHEALLEFVIKLMENVLINTKLEVKD
                     AYEIK"
     gene            complement(77211..77795)
                     /gene="pth"
                     /locus_tag="PLQ68_00390"
     CDS             complement(77211..77795)
                     /gene="pth"
                     /locus_tag="PLQ68_00390"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008395535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HXK71138.1"
                     /translation="MDRYVITGLGNIGLKYRNTRHNIGFMVIDELSDIFNIKVKKNHC
                     LANVGEGIIKNHPVVLAKPRTYMNLSGQSIRALMDWYKSDISRLIVIYDDMDLPLGDV
                     RIRYKGSGGTHNGMKSILNHLGTNQFIRIRIGIGRELNEDAVNYVLGRFPKQEKQIIS
                     QALDTAVKAITCILSEGIDIAMNKYNSKKDKGDV"
     gene            complement(77795..78775)
                     /locus_tag="PLQ68_00395"
     CDS             complement(77795..78775)
                     /locus_tag="PLQ68_00395"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006313884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HXK71139.1"
                     /translation="MAKNDIKIMGGNANLKLAAQIANYLGVPLLESKVGRFADGEIQM
                     LINELVRGSDVFIIQSTCPPVNEHLMELLIMTDALKRSSSARVTAVMPYFGYARQDRK
                     ARARDPISAKLVANLLTTAGVDRILTMDLHAPQIQGFFDIPVDHLEGSIIFSDMVLDL
                     MKGKDKSDFILVAPDTGSVNRIRKMADLTELPIAITDKRRTRANECEIMNIIGDIKGK
                     TAIIMDDLIDTAGTITKAANVVKEMGAEKVFVYATHGILSADAVEKIKDSSIEKMYLL
                     DTVPIPESKMIDKIEIVEVAKLFSEAIYKIYTNTSMSALFKFPPSIGTEK"
     gene            complement(78811..79731)
                     /locus_tag="PLQ68_00400"
     CDS             complement(78811..79731)
                     /locus_tag="PLQ68_00400"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HXK71140.1"
                     /translation="MDDKRFTIKEMEYFSAYRENMVFGVTPKEILEDKLFSLGYYYDE
                     SGLSVPEDIFYCPVSFIGQYMKKKGKLEFEPVDDPISYYEAYEHVKTQINIGHIENGV
                     RMLSVTETYIDYTVKIAPKAVIYPNCFIEGDSYISEDCIIGPDTKIESSRVEAGTSVI
                     KSVVLESSIGQNCTIGPFAYIRPHTRIKDKVKVGDFVELKNTTVDNNTKIPHFVYAGD
                     SEIGKACNISCGVITANYDGKAKYKTKIGDNVFVGCNSTLVAPIELEDNTFIAAGSTI
                     VDDVKTGTLAIARAHQVNKEGWVEKTGRMK"
     gene            complement(79818..82121)
                     /locus_tag="PLQ68_00405"
     CDS             complement(79818..82121)
                     /locus_tag="PLQ68_00405"
                     /inference="COORDINATES: protein motif:HMM:NF025851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase N-terminal domain-containing
                     protein"
                     /protein_id="HXK71141.1"
                     /translation="MSDYMIKEMPVAKWDEAVPLGNGKIGALLFGGIFREKIVLNHER
                     LYNKSEPVEIGAISHNMEVIRKLILEGQYSKAEQLYKKSVDDSYENKKGIDPYQPLGF
                     MYIGQEIKKVFSDYIFKLDFKKALAEISWKEDEVLLKRSTFISYDEDVICVCIEASEE
                     GYITARVNIEGGSESKGVMEYTLDTDDDFLVYKVKYPNGSNHGAVAYIKGINGIKTHD
                     EKNIRIRKSDKTYIFVKTYVNEDDNKAVSRIKKQLLKCDYEKLKRSHTKKWKQLYGSV
                     ALDIGQSRKAKSNEQLLKEAYNQKASKEIIHTLFNYGRYLLISSSKKGGLPANLQGIW
                     NGDYNPAWSSDFHLDENIQMNYWAALSCGLNDCTMPFYDYFEQFIEDYRQNAKRVYGA
                     KGILLPLAQTTDGKFNSHWISASGWIAQHYFDYWLYTADDDFLVNRAIPFMKNVVEFY
                     EDYLIMDNSGTYVFVPSYSPENTPVCENRSSICVNATMDVAIAKEVLTNLIYAYKHLD
                     ITDDDSEKWQDMLSKMPEYMINEDGAFKEWLHEDLKDNYYHRHQSHIYPLFPGYEITK
                     QTSLEYYEAIKKAVDKRLLVGFSEQTGWSYAHMANIYARLENGYKASMCLSNLIKSCV
                     KSNLFTFHNDWRGQGVTADWFGGNPPFQIDANFGFTAAVLEMLVFSKPAYIRILPALD
                     EKWEKGSVKGIKTRCACSVDIKWDIVKKRITVTLNPIKSSILTLEIPMGFSDISNSGC
                     KIINKRDKKYVDIEIATGTTAVIEVSI"
     gene            complement(82130..84457)
                     /locus_tag="PLQ68_00410"
     CDS             complement(82130..84457)
                     /locus_tag="PLQ68_00410"
                     /inference="COORDINATES: protein motif:HMM:NF025851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase N-terminal domain-containing
                     protein"
                     /protein_id="HXK71142.1"
                     /translation="MSDYMIKKLPAVDWQEALPLGNGTVGAMIYGNIQKERILINHER
                     LFRYQKEPIYDPFSKHLNDVRKLMLDEKYDEGEKYFNEKFKECCKEVYNVDPYQPFAE
                     LSIFRKPDDAFADYKAVLDFQSAIATVSWKEKNTKIVRESFVSYDTGCICTSMYSSDD
                     KLLSIKVNVDQVDGGDEYMDSKCEIKNDCLIYEARYKDKREHGAVIYVETTGGNVDFA
                     KDTIIVEKAKVINIYTMLYIYEDADKAVNRLIKQIKKYEYNQLKKKHISTWKKLYGST
                     DLDLYSDNKNLSNEEYLLKAYNDEKPLNLFHTMFNYGKYLFISSSRKNGLPVNLQGIW
                     NGTYNPKWDSDIHNDENVQMCYWASLALGQFDSSMALYDYFDSLIPHFRENAKKIYGC
                     RGILVPICMSTHGKISGMTGRWQSWISAGGWLSSHYYDYWLYTKDDDFLKHRAVRFMK
                     EVVLFYEDFLITGEDGKYIFVPSLSPENVPNVEHASMMCVNATMDIAVAKQSLTNLIT
                     ACRYLDIEQENLARWEAMLDKMPDYEVNEDGAMKEWLYDDIKDNYYHRHQSHIYPLFP
                     GFEITKESNGKIYNAIKTAVDKRLTIGFVQQTGWSYAHMANIYARLEDKDNAYACLNY
                     ILRACTGSNLFTYHNDWRDQGLTVYWFGQHPPFQIDANLGFTAAVLEMMLFSKPGFLR
                     ILPALPDQWKKGRIKGIHTRTRTKCDISWDKSDIEIAIFSLEDQKITIQLPVIPNNIN
                     STCKNIELINTDLQNNYSYYSVELKKDEKCIITVK"
     gene            complement(84540..85133)
                     /locus_tag="PLQ68_00415"
     CDS             complement(84540..85133)
                     /locus_tag="PLQ68_00415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK71143.1"
                     /translation="MVKISVVLKKNDKYIVRKEFFEEDLFYGFWEVNSRNMNEESNDI
                     QHVNSMFEELYGISICKTSFLTSVSNPETRYDFYLSESDKEEPINFSSYEIVNEEQLI
                     SLNFIYPDALCRDKLVSSEKSLSQSEKDILIAADKLFHIRKNAARADTFIELMNNHIT
                     VSEFEEYMLSRIETYKYGMPHFGFADDSETSLCIWKE"
     gene            complement(85126..85566)
                     /locus_tag="PLQ68_00420"
     CDS             complement(85126..85566)
                     /locus_tag="PLQ68_00420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK71144.1"
                     /translation="MLAEFLGAVIKKDSRYLVETIEMNGHMYVMFPRVETINAIAGKQ
                     DILSYGIKEKFKFDISVGKCIYQEDFKDGLDVISYAYFEACIVKGEMPENLLWIEEAD
                     LDKADFMLGDDIVLMHISKRKKTKEEILESSCKYLYDKKEMRNG"
     gene            complement(85665..87329)
                     /locus_tag="PLQ68_00425"
     CDS             complement(85665..87329)
                     /locus_tag="PLQ68_00425"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HXK71145.1"
                     /translation="MLTDIEIAQSALIKPIKQIADSIGIAEECLELYGPYKAKVTSKA
                     VQGRDKDGKLILVTAITPTPAGEGKTTTSIGLADGLRRIDKKSMLCLREPSLGPVFGV
                     KGGAAGGGYAQVIPMEDINLHFTGDIHAVTAANNLLAALIDNHIYQGNLLGIDPRRIT
                     WKRCLDMNDRQLRYTVDGLGGKTNGMPREDGFDISVASEIMAILCLSQSIDDLKQRIG
                     NIIIGYSYDNEPVTASMLKAQGALAALLKDTIKPNLVQTLEGTPAFIHGGPFANIAHG
                     CNSVMATKYALKLSDYVVTEAGFGADLGAEKFFDIKCRMAELKPNAVVIVATVKALKY
                     HGGVNKKKLGDTNVQAVKDGMDNLYKHIENITKVFKVPAVVAINKFSNDTLEEIEVIK
                     KGCESYGVTAVECLVWGKGGEGGIELAEKVVELCDKQNEFEYCYESDMTITEKIKAIA
                     TKIYGADGVEFSSEAKSMLKVIREKGYDNLPVCVAKTQFSFSDNTKLICRPSDFVITV
                     RDLKISAGAGFIVALTGEIMTMPGLPKVPAAESIDIDNEGKITGLF"
     gene            complement(87395..89776)
                     /gene="priA"
                     /locus_tag="PLQ68_00430"
     CDS             complement(87395..89776)
                     /gene="priA"
                     /locus_tag="PLQ68_00430"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HXK71146.1"
                     /translation="MEHKTVAAVILDNTTRTYDKIYNYLVPDDMLLYLETGMRILVPF
                     GKGNSTKVAYFLDFVPMPDKLKNVKMKYIIEILDDEAILSKNMFILAKTIRDEYFCTY
                     GQVLNAMVPLGLKISVEKLVLQEDGTLTLFQTYLKSLSGGIKQLNEQMKSNKIKIVEQ
                     GIRNIKKKFEKAARLKIEPSFVSELIFKDQIKNEKHIRALELLSSYEDITVNDFASYE
                     NISRNILSTLEKKGYIELFDKPKKTDIHVEITDEALDSNKIPTVEQKNVTDTIKQSID
                     KEIYDRFLIHGITGSGKTEVYLNLAAKVIQKDKGVIVLVPEIALTPMMVRRFSERFGN
                     NLVVMHSRLSMRERYNAWMRIKRGEVKIALGTRSCIFAPFSNLGLIIIDEEHDNSYIS
                     ESTPKYHTNFVADNRAKMENAVLVLGSATPKVTTFHAFERIGRVLFMQNRASGRLPEV
                     SIVDMREDAINGKMSVFSNTLIDQIKKNIDNHEQTVLFLNKRGYTSMQLCTSCKHIIK
                     CEHCDVPMTYHKGRDYLICHYCGHIQKKPIICPNCNEQTLMECGVGTEKCEAELKELF
                     PDASVIRMDLDTTGMKNSHKEILERFEKDKIDIMIGTQMIAKGHDYPLVTLVGILNAD
                     ALGAGSFYESNENAYQLITQSAGRAGRSDLKGRAIIQAYNVDSALMVSAIEQDYDNFY
                     KSEMKIRKVLEFPPYTNMAFIIFSSIQQHLSEEYAEKAYNLLKKTNMVVYSPSKPQIS
                     KLNDKYRTRIIVKHTNTKQFEENLTKAYMLMMKKLPKSVNMSIDVYKDEFGIL"
     gene            complement(89780..91219)
                     /gene="proS"
                     /locus_tag="PLQ68_00435"
     CDS             complement(89780..91219)
                     /gene="proS"
                     /locus_tag="PLQ68_00435"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011722820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HXK71147.1"
                     /translation="MAKEKKDVEQITKREEDFAKWYTDVVRKAELIDYSQVKGCVILR
                     PYAYALWENIQKILDSMFKNTGHENVYMPMFIPESLLQKEKDHVEGFAPEVAWVTHGG
                     SEELTERICVRPTSETLFCDHYKNIITSWRDLPKKYNQWCSVVRWEKATRPFLRHREF
                     LWQEGHTIHSTVKEAREETQQMLAIYAEFAENYLAMPVVKGRKTEKEKFAGAEETYTI
                     ECMMHDKKALQSGTSHYFGDGFAKAFDIKFTDKDNTLKNPFQTSWGMSTRIIGGIIMT
                     HGDDSGLKLPPMVAPIQVMVIPVAQHKEGVIDAADALLQRLKKAGIRANIDVSDQSPG
                     WKFAEYEMKGIPLRLEIGPRDIENSQCMSVRRDTLEKQPLCLSDIENTVKDLLDEIQK
                     NMFIEAKQNLDNNTFKAETIEELKEIIGTKGGFVKAMWCGDEECEKRLKEEAGVTSRC
                     IPFDQEEFADKCVCCGKQAKHMVVWAVAY"
     gene            complement(91223..91693)
                     /gene="nrdR"
                     /locus_tag="PLQ68_00440"
     CDS             complement(91223..91693)
                     /gene="nrdR"
                     /locus_tag="PLQ68_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016323590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HXK71148.1"
                     /translation="MKCPNCGHEEDKVIDSRPSEEGYAIRRRRECLNCKERFTTYENI
                     ERKPIVVVKRDQSRQNFDKAKLLNRLMIACAKRPVPITMLENVVNAIELDFHKQHLRE
                     VTSREIGDKVLEHLIDIDYVAYVRFASVYKDFDDMDTFIKEIHLIKEKQKKQEI"
     gene            complement(91835..94096)
                     /locus_tag="PLQ68_00445"
     CDS             complement(91835..94096)
                     /locus_tag="PLQ68_00445"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HXK71149.1"
                     /translation="MSQNNSILENLNEEQLRAVTNTQGAVLVLAGAGSGKTRVLTRRI
                     AYLIQKGLARPNQILAITFTNKAASQIKQRVYKLCEEGLEGKFFWLGTIHSTCLKMLR
                     QYPNEAGFKSNFLIYDTDDKKSVIKNCMKQLNISDKAFTPNYVAAEIGRAKDDLMGPD
                     VYQAAHSHDFQKKAVGNIYKLYQEKMKEYNAMDFDDIIFNTYYLLKNNQYVLEYFQNI
                     FKYILIDEFQDTNIAQYRIFAMLSQKHKNLFVVGDDDQSIYSFRGANIKNILNFEKDF
                     PDSTVIKLETNYRSCENILKAANHVIKNNVSRKNKTLKTSNPQGCKINVYKAYNEMNE
                     AIYVASEIKKMINQGYSYKDFAVLYRTNAQSRVFERVFRERDLNYRIFGGLSFFSRQE
                     VKDVLAYMRVVFSTDDDASLFRIINQPKRGIGDTTIKRIQYISSQTQLSSYEIIKNAK
                     DYEQLSSASAKLEAFADIIEELKQYDKPGKLDQLYDMILKLTEIDEHYIREDSIYGRE
                     RIENIKELKSAITTMQQSSEEESGQELSLSVFLENVTLSTDVDKEDDSDDHITIMTLH
                     SAKGLEFKVVFLVGMEEGLFPSSMSVTESLEGLEEERRLCYVGITRAMEKLYCIYAEE
                     RTKFGKTQPCDQSRFLKEIPYELIEGGKKKHKSDDSNIFSFSKMFSSDNTVSITKGGK
                     PVDIPGLTTKTVNHMEKADYNIGDRVIHKKYGEGSIKDLYDDGGMDIVEVDFDIAGVK
                     RLVLEFAKLQKID"
     gene            94231..95328
                     /locus_tag="PLQ68_00450"
     CDS             94231..95328
                     /locus_tag="PLQ68_00450"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HXK71150.1"
                     /translation="MTSQLTSRERFVRTLTGQDVDRVPFMRIFGSDNYALSSWLKYRP
                     NLSLYMDKLIGFEGTYRGWATIPVNYGLCGLPPDICLDDNGPIHIYKNGIGEIRTWSK
                     DRDYHSGVLEYALKDANDWDRIRPYVVCDIEKRIPSDIDELSEFYRHRDYPLQLTCGG
                     VYGFIRKLAGDENLGYLFFDDPDLVKEIISEYIDMLLNLWERLCEKIEFDLIESWEDM
                     AYKCGSIISPEHFNMFLAPHYRKIRKFADAHHIPILLVDSDGNTDVLAQWMYDSGVNS
                     IYPFESLAGCNPKNLRAKLPNMGGIGGLNKDSMAHGKKEMDEELEKAIELIKLKRFIP
                     GPDHMVLSNVSFENYRYFMTRLKDIVMNTKP"
     gene            complement(95454..95990)
                     /gene="raiA"
                     /locus_tag="PLQ68_00455"
     CDS             complement(95454..95990)
                     /gene="raiA"
                     /locus_tag="PLQ68_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020814177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HXK71151.1"
                     /translation="MKISIVGRRIDITEEVKDWTEKKFAKLDKFFDASTEATVVVEAL
                     KQNKHAKVESTIVWKGVIFRAELSGNDLLGQIDKSVDIIERQVRKNKTRLEKRFHGDV
                     PKNWKGISQETEFSEADYNVVKTKKFPVKPMSVDEAVMQMEMLNHEFFMFVNEETNVM
                     NVVYKRKDGDYGLLVPYE"
     gene            complement(96089..96829)
                     /gene="truA"
                     /locus_tag="PLQ68_00460"
     CDS             complement(96089..96829)
                     /gene="truA"
                     /locus_tag="PLQ68_00460"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HXK71152.1"
                     /translation="MLKNILLIIEYSGKNYSGWQRQLNGISIQEKIEQSVFELTGQTI
                     KLNGSGRTDAKVNAMGQTASFKIDCTIPPSNFCMALNSVLPEDIRILESFEIDMDFHA
                     RYSATGKHYRFTILNRSVPSAIYKYTTCHVKQPLDVDKMLKAASYFIGEHDFRAFMSA
                     GSSIKTTIRTIYTLDISQDGPLIYFDIQGNGFLYNMVRIIAGTLAEVGKGAIEPEQIP
                     EIISSKDRSKCGPTLKASGLCLMEVFYG"
     gene            complement(96913..98184)
                     /locus_tag="PLQ68_00465"
     CDS             complement(96913..98184)
                     /locus_tag="PLQ68_00465"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HXK71153.1"
                     /translation="MDNRYNVNDNNNPTNLYYQYNNAAYANVQEKKRMSTFKKTFIIA
                     IAICSVFSLLVGLAGGYVASRYYQKAVELNKLYENQDVLNSQSSKPSGENDSSSIYTS
                     SSTIKEYDSVADIYADNVNAVVGIVVEFETSSVWSTYTSTSSGSGVIISKDGYILTNN
                     HVVANANKITVYLNDKESYEAELIGLDVQLDLALLKIDADDLEYMVIGNSDEIVVGEL
                     VLVIGNPLGYLSQSLTVGYISGLEREITLDGEKMVLLQTDAAVNPGNSGGALINTKGE
                     LIGIINAKSYGSEIDGIGFAIPINDIKNVLDDMINYGYVKGRPYLGVSLGEITSQNDL
                     KLYNMMYGLNLTEIGVYIGMVEEGSAADKAGLQEWDYIKSFKGTEISSSSQFKELVDE
                     CEVGQIVDIVIVRDENTLMLTIEIGEEIPNK"
     gene            complement(98532..99398)
                     /locus_tag="PLQ68_00470"
     CDS             complement(98532..99398)
                     /locus_tag="PLQ68_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004620791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HXK71154.1"
                     /translation="MHFTQAKGILSSYNSMNMYRGCTHGCIYCDSRSKCYNFTHDFED
                     IEVKINAPQLLEKALITKRNKCMIGTGAMCDPYLHYEKDLMITRKCLKIIDHYGFGVA
                     IQTKSDMILRDIDILASINEKVKCVVQMTLTTYDEKLCKILEPNVCTTKKRYEVLKVL
                     QENKIPTVVWMTPILPYINDNESNIRGVLEYCFDADVKGIINFNMGTTMREGDREYFY
                     KALDDNFPGIKQKYINRYGYSYECISDNNDHLMEIFRNECESHNVMHNLSLIFSYMNE
                     IPTKNTQISFFQ"
     gene            complement(99415..100866)
                     /locus_tag="PLQ68_00475"
     CDS             complement(99415..100866)
                     /locus_tag="PLQ68_00475"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HXK71155.1"
                     /translation="MGFERLYIRAKHNELIEYGEEINGYFAEIDISTPDFRDNLINAF
                     DKIERNNIEIIWFVYDETGMQVDIGYSTKITGQYLPNDTPDIKALEDYIGVLITNGEL
                     ADITKVYGYKTDETNVGGFPSINLITMVYENTDSTNGNIVRSYIVLNSPKEYISIVAN
                     TAERFVMYVSIITLLIAVIAVIIISDKITKPIVNINKVANKISNLDFSETCEVSGNDE
                     IGDLGNSINEMSRKLQDSIDYLKQRAIMLRNDLEKQESSNKTKKEFIASVSHDFKTPL
                     SLITAYSELLVEKYKDDEDTKSQLMIISEQAMKMNLMVNQLLTLSQLESGTAKIDDSI
                     FCLNEAVESVLKDIRIMIEQAEIKLTFKYQDDYIVKGDYNKITHVITNLLENAIKYCD
                     EKKIVNINIKEIDNKIRTSIYNTHPGISEQALPNLFNSFYKEDKSRGGNSKSYGLGLS
                     IVKAIMEMHNGNYGVYNSNAGVVFWFELEKHSD"
     gene            complement(100950..101615)
                     /locus_tag="PLQ68_00480"
     CDS             complement(100950..101615)
                     /locus_tag="PLQ68_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016441454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HXK71156.1"
                     /translation="MLNKILIAEDEPALRNLVKTCLVKEGYSVIEAKDGQEALDLFYS
                     ENVSLVILDIMMPELDGFEVLKTIRIESTVPVLMLTARNTEYDELHGLKLGADDYITK
                     PFSPAVLVSRVKAVLKRSGMFADNDISANDILLKVREHEVYVKDEKINLTPKEFDLLQ
                     YLIQNKNIVHSREKLLSAVWGYDFFGDARTVDTHMKCLRTKLKQSGDCIKTVRKYGYK
                     LEG"
     gene            complement(101727..103514)
                     /locus_tag="PLQ68_00485"
     CDS             complement(101727..103514)
                     /locus_tag="PLQ68_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HXK71157.1"
                     /translation="MNFFSVITIFAGLTLFLYGMSVMGSGLERLAGRRLESVIEKATN
                     SPLKGVLIGAAVTALVQSSSATTVMLVGFVNSGLMKLSQAVGVIMGANIGTTITSWIL
                     SLVSLDSDAFIIKLFKPDTFAPLLGIAGVILLMAGKRNKNKDLGRVLIGFFVLMFGMS
                     TMSSAVKPLAEVEGFTSILTLFSNPLLGILTGAIFTAVIQSSSASVGVLQALSTTGAI
                     TYGSAIPIILGQNIGTCATALISCIGTNKNAKRAAMIHLYFNLIGTFLFIGLFYGLNA
                     ILKFTFMDSSLNTVGIAVVHTLFNLLSTLVLLPFSKLLVKFAEKTVKDKGVSPEKIIL
                     DERFLNTPSIAVDQCKKHILNMANITKETISIALPLINNYDPKKANIITANEDMIDLY
                     EDKLGSYLVKLSGRELNDRDSKEISKILHSISDFERISDHACNILEVAKELYDKNITF
                     SPQANQELKVMADAVEEILVLSMDAFINNDFEKAYKVEPLEQIIDELKLILKAHHIER
                     LQGGNCTIELGFILSDLISNYERVSDHCSNLAVLTIQISEGAFDTHEYLHDVKKTDQE
                     RYMQIYNEFSAKYDVTKLPVIGEVIDTVK"
BASE COUNT        30482 a        21083 c        15050 g        37116 t
ORIGIN      
        1 tattgaaaac tttggtataa gtgcttgatg taataataat gcttattcaa atattttgaa
       61 ggtccagccg tcatttatca attcatcaag aaatactggt aaggcttcaa tgttatcagg
      121 tgtaactata tgaagctgat aaacagctcc ttcatgaaca tcctgtgtaa gctcattgta
      181 tgcaaattcc gcaccattaa ttacagttct gttccagtcg gaatatgtag catcaaataa
      241 aactgtcgta tatccaagtg attgagctat atataagtct ctttcacaaa attcacctat
      301 tataggccgg taatatctca tcctttcata atcatcttta agcgctattt caagcagctt
      361 ctctgtttta ataaaatcat ctatatactc ttcatcagtc attgtatatg agggaggtat
      421 atggtagtag ctgtgagctc ccaaggtgtg tccgtcatca tatattgctt taactttttc
      481 aggaaatatt tctatgaatt ctcctacaag aaaaaatgtt gccaaaatat ccctttgttt
      541 aagtaattcc agcagttttt cagtattacc acgaacatgc tcatatccta aattaaaagt
      601 aagataaatt tcctttttct caggatccat cgatgttctg acattgtatt tctccatgac
      661 ttctgttttg ttactctttt caccccagct tatttgttct cttgataatt catcggttac
      721 agtaaggtca atatataggt cataatagtc tatatatgtg ctgttaggcg gtatgactgt
      781 agggcttggt tcaatagttt caaaagtttg tgatggttca gcctgagttt gtgaaggctg
      841 gtctgtttca gattcagaag gtggtagagt tacagttata tcaggggtaa ctgtatcagt
      901 aaaagatggc gggactgttg tattatcaca tgatgataat aaggttatag cgcatatgag
      961 caatattagt aaagtgttct ttctcattat tcctacctta tggttatgtc ggcatctgtt
     1021 ggtatacatg atatccaaga atttccctga gcaacctgta acggttcatt gttatatgta
     1081 aattcaaatt ctgaagagtc agtttccctg ctccattgta tctcaatata ttttccatca
     1141 tttatgtaat aaccgctacc tgaaccatat gtggtaatag ctacatgctc agaagcatca
     1201 cctagatatc tggtatccat ttttagcaaa aataagtttt caacgcttat agcttcaccg
     1261 ctgttgccat caatatgctt atctgcaaac tggtatcttg tatactctga tttgttatta
     1321 tcataaataa aatatgggct gcttatatat atacctgtaa ggtagacttt atttgcatca
     1381 tttccttctt tagtactact ttgcgtagaa aaattgaata tgttcttttg ttcagttttt
     1441 tcaatctttt ctgttgaaat attctgcatc gatcttaata cattctgccc tgctgtaact
     1501 acacagtgaa cagactccat tccagatgca attctgtcag agtctctcca gaaaggatat
     1561 ccgttatctt ctccaaatat gccgtttatc gtagtaagtc ctctgtcagc taaatctttt
     1621 ttagcattct cttcaccata ttcactgaaa ccgtaatgga atatgtaact gtcaaatgcg
     1681 agtgccattt ctgtaaaata tgaacgcaag cttcttacag ctcctatctt ttcaacatta
     1741 tatggatcct gatatatcgc cataaaacgg gaaatatcat actctacaag catttctata
     1801 actatatccg cttcagaaat gcctgcctga ggcagactct gtctcttgtt ttccaaaata
     1861 atcgctaccg gtttaacatc aagtgcaaat tcatgcatat actcactctt acctgtaaga
     1921 ggattaactg ccagcaccaa tgaaggatca attgtagctg aaggctcttc tgttggtgta
     1981 acgctttcag taggcgtttc agttggctct actgactcgc ttggttgctc tgtctgtgta
     2041 ggtgttacag atggtctagg cgttttactt ggaagagttg gtgattctgc aatattccct
     2101 actgcttcaa agtaactgcc acaagaagaa aacagtattg aaattactat tatcagagaa
     2161 attattattg tttttcttaa aatattactg atttgtcttt tgtgtttcat ctcaattctc
     2221 catatttcaa acggaattat atctcatttt gaatatattt tcgctcaata attttgttat
     2281 gtcaaatatt ttagcggtat attatcctgg tttagtatat aaagctggaa atatactctt
     2341 aagtaccata atgtcgtaaa tattgtaaca gcagaaacaa ttgccacata cggagaaaaa
     2401 agatcatatc ctccattgaa attcatatac agaatatatg cgcagtttgc aaacactgta
     2461 atactctgaa gtatatgaat gtacaagaac cttttgtcat ttgagaatat gtatgctgcc
     2521 agtacaagca gtatagatgg aaacagatat ctttcatgca tttttgttgc aaatgcaaaa
     2581 accacagtca ttaaaagacc tgctgttgca aaagtaaatc ctggcatctt gtttcctttt
     2641 ataaaagcta ttgtagtcaa agcacatatt cctaaaatag ctacatatcc atatatctta
     2701 tatgcaaaca aaaacataac ttcactatct gatacccagt tcaaacctaa taaagcatat
     2761 atgttatatg cattaactgt cgcatatgca tattgtccca ttgttccaaa gtataaagag
     2821 aatatccaca aaggctcctg ccgtatagca aagggaagta ttacaacaac gaacagcgct
     2881 aaacctataa gcaaggatat aaggactttt tttgtatttt tgcttttaat gtatgcgaat
     2941 atgagtattg gcgcaaatat aaatgcctga ggttttagaa gtactgcaat aacataagca
     3001 acagttgcaa actcgagttt gttattcata atgttgtatg ctgttagcgc tataaaaaag
     3061 gtcagtatgc tgtcaatctg tccccatgct gcagaattca atatgtatgc aatatttaac
     3121 gctacagcag cagaaagtat aattgatgtt gtttcagaaa atcttgtttt tgctatctta
     3181 tacactaaat atgcagtcat aatatcaaaa atgatagctg gcattttcaa caggattata
     3241 tgagctggaa atgcgaatgt cagaccaagg gcttctcttg caaattctat aaaccatagt
     3301 atgtatatat acaaaggagg ataatcacag taatatgaac cgttatagaa ctctaataat
     3361 ccgtcattgt aagcctgttc accccagtag ctgaaacatc ccatatctgt aagatgcccc
     3421 ataacagctc ctgcaataaa tacacgagta ataaaagcta ctaagaaaac cgctcctaga
     3481 ataagataag atttattaag tttttctatt aaactattgg tcggtttgtt ttctttggta
     3541 ctcatagaaa gtaatatgaa ccccgctttc cgtcataagt ctgtcggata tgtgcacagc
     3601 agtccattga ggctcgttgt ctaagtttat aaaatgttta tccgctttga aaatcccatg
     3661 gacttttgta acatatgctt tgtcacaata tggaagaagc gcttgatata tactctcacc
     3721 tccaataaca tacacatcag aactattttc attttctatc ttatttaatg cctgctcaat
     3781 ggtgtttact actattacat cgtctcgttt gaaatccata tcacgagtaa gaacgatgtt
     3841 tttcctattc tttaatggct tgccattttt aaaagaatca agagttcttc tccccataat
     3901 aacagtttta tccatagtct tatttttgaa atatgccata tcctctttaa tatgccattg
     3961 tatcttaccc atataaccaa ttccccagtt ttcatctaca gctacaatta aattcatact
     4021 gctacctcaa tatccttaat ctgaggattc ttcttgtaat caattagttt aaaatcatca
     4081 actgtaaagt cataaaagtt tgtaatttca ggattaatcc agaacttagg ggcaggataa
     4141 gcaggtcttt ttaaaagttc ctcgactagt ggtatatgtc tgtcatatat atgtgcatca
     4201 gctattacat gcaccagttc tcccgcaatt agtccagatg cttgagctat aatatgcata
     4261 agaacagcat attgaactac attccagttg ttagctataa gtatgtcgtt tgatcgctga
     4321 tttagtatac cgttcaatat gtttcccgaa acattgaaag tcatgctgta tgcgcaaggg
     4381 tacagattca tttcatgaag gtctgcatga acatatatat ttgtcattat ccttctgctt
     4441 gccttgttat ttttcaaatc atataagact ctgtcaacct gatcaaacat tccttcattg
     4501 tatttatgct ttacagacaa ctggtaaccg taagccttac ctatagaacc attctcatca
     4561 gcccatgcat cccatatctt gctattcaaa tcatttatat tattagattt cttctgccat
     4621 atccacagaa tctcatcaat cgagccctta aaacgtgtag gacgtaacgt aagtatagga
     4681 aattcttcag ataaatcata tctgttaacg acacagaatt tttttacagt gtgagcaggt
     4741 actccgtcta gccattttgg acgtacatcc aaatctttat ctgaaaaccc attgtcaata
     4801 atatctcgac acatattctt aaaaatatcg tctgctttac tcatttaccg ctcctttctt
     4861 cattctttta atattataca atcaatttgc tattatgtga actaaatacc tttgtaatat
     4921 ccttttaatg ctttaataac atattttgca attatacctg tcagtatgcc tgttactact
     4981 gcaaatacag ataatatggc tatgtaccag aatatgttta tatttcttaa aattactagt
     5041 gcagttataa actgggcaag attgtgtaca attgctccaa ttatactaag gcctataact
     5101 gaaaactgta tctttttttg ttttttaatt agaacgcata ctaaaattga tgccatgcct
     5161 ccaaaaaagc tgaaaagaaa tgtaacgaaa tttcctctta aaaggcctgc aattattatc
     5221 cttacagctt gtactgctac tgcataaaca ggtccgagca catataaaag tgttaaagga
     5281 actacattcg ccaggccaag tttaactcct ggtgcaatcg ctgaaaaagg gatatatccc
     5341 tcggcaatgg aaagagatat tgccaatgca gtaaatatag cggatattgt aatctttttt
     5401 gatttactca tcttgttata ccatccacta ttacattatc ttccgtcagg tatattaata
     5461 ccttgtttgg aagacatgct atctgctttc ttgaaccgct ttgtatatat cccatatgca
     5521 tacagtcttt attaggacaa gttgcaaaag acatatatgc tttcccatcc ttaattgtaa
     5581 cggtatttat taaattacca ttgtcatcat atatgtttac tatgttatct tcatcaatgg
     5641 gttttctata tacaatttca ttatcctgct ggataatgag aatagaatca tctttattat
     5701 ctgttaaaaa atagatggat ataaaaatga cgaatacgca tattaagatt gctatcagtg
     5761 caatatcagc tttcttcata tgcgtattct atcattattg gtatttgttt aaaagttctt
     5821 tatatatctg ttcatcagct gtgctcatgt ttgacggatt tactatattt acattgatgt
     5881 ataaatcccc tctcgagcca tcttttttat aatatccttt tcccctgact ctaatacgtg
     5941 aaccgccttt aactttctga ggaaccgtaa cttttaaggt cgtatccggc atgtttattt
     6001 gcacctgcga accaaaatac gcctgccagg gatagacatc agctgtcttt ataaggtcat
     6061 ctccttcaag agataaagta gcagtattct gaatatcgaa cttaataaac aaatctccgc
     6121 cgttctgccc ctgacctttc atctttattt tctgaccagg tcttatacct ttgggtatct
     6181 ttaaattaac ctttttgtta ttaattgata ctaccatttc cctacctgat aaagcatctt
     6241 caatactgat tgtcatcgag gcctcagaat caggctgcga aggcgatctt cttgcagatt
     6301 ttcttgatga tgatgaaaaa ggatctgctc ccattccacc catacccagg tctgcaaaga
     6361 atgcgttaaa aaagtcagaa aaatcattag attgggaaaa tccagaacct cggttaaaat
     6421 catatgagta ttgatttcca aatgatgacg gatcaaatcc cttaccattg aatccgtttg
     6481 agcccaaagt atcatagcgc tttctttttt catcatcaga aagcacttca taagcctcat
     6541 taatttcttt aaacctttct tcagcccttt tattgttagg gttgttatca ggatgatatt
     6601 tcttggctag tttcctatat gcttttttta ttgtatcctt atcgtctttc ttctctactc
     6661 caaggacttt gtaataatct ttatattcca tatgcatcac ctcggtctga ctttctctga
     6721 ccttatttta ttatataact gctcaatact tatttcaata gttatttata ataacacttt
     6781 atccatagca aaaattacca tatgaaatat atggtaatta aacgtatatt tatatcggtt
     6841 acaggcttgc ttgaatcgtt agtattatac gctgcagaat catcactagc acatacaaag
     6901 ctatatatga gaaatcaaac atcataggtc tacctttagt aaaccttgct atgaggtttc
     6961 tcaatggagc cagaaatggt tctgtaagcc ttactaatac ctcatagtat atgctgaatt
     7021 tggcacttcc aaaaaacgat gcaatcgctc taataaacac cagcaaggaa atagcatata
     7081 gcaaatagta taggactgtt agcaggatat taagcattac accttctaat tagcaaatgt
     7141 tcttgttttg gtatcagcta actttgacag atcctgcttt tcatcatcga tatgatagcc
     7201 gctaggagca acgactaata tcttatcgga cagttttctg atagcgccat tcaatgtata
     7261 tactgtgccg ctaagaaaat caactatcct ttgtgcaagt tctttctcaa tctgttcaag
     7321 attaactatt accgctctgc ctgatttcaa tgaatcacat accctctttg tttcttcgaa
     7381 actttcagga tacataacat ttaatatacc tgtagaacgt tccatgggaa ccaccttctt
     7441 gcttgcaatt tgatctaagg catcgatcct atcaactata ggactaactt gaacagatgg
     7501 tctggttttc ccattaccat ctctagttac tggtgctact tgttgttttt ctggcgcagc
     7561 ctgttcgaaa cctaagaatt cggttagaaa tttgtcaaaa acactcatgt ttttcctcct
     7621 cttttgtgta caatcaaaat aatctttagt atttatggtt tatctttaaa aattgctgaa
     7681 ccaacacgta atatattagc gccctcctta gcagctaaga tataatcgtt gctcatcccc
     7741 attgaaagat ataccatatc tgtattgtaa aaggattttt tgcttatgtc aacaaaaata
     7801 tcatgtaatt tcctaaatgt atgcgatatt aactcattat cctgtgtaaa aggcgccatt
     7861 gtcataagtc ctttgatctt aacatttgga ttattctcat tgtgggatat aagtttttct
     7921 atgtcttttt catctgctcc aaacttatta tcatctttaa atatctttat ctgaagtaaa
     7981 acatttgtaa caacctgctg ctgagatgat cttttttgta tttcatctaa aagttcttca
     8041 ctatcaactg aatgtattaa tacagtttta ccgactatgt attttacctt gtttttctga
     8101 agatgcccaa ttaaatgtat ttttgcctta ccttgaaaat agtcatactt ttttaaaaat
     8161 tcctgaactc tgttttctcc cagttcaata ccaggaacta ctaaagctaa agcagcagtc
     8221 tcacaggaaa ctgtttttga aactgcaact attaagatat catcattatt cagctgcttt
     8281 ttgattttat aaatattttc tgctacgtag ttaatcatta caaacctctc tagttaacaa
     8341 catcaccatc tgaaacggaa tatggatcta gtatataata atcatacacc ttgaaactgt
     8401 aatcctcatc tatgtctttt attatggcat atataccgtc agtataaagg acatctatat
     8461 agacaaactt tacagatata cctttaacaa gagctacctg catattttga aatgaagaat
     8521 atttatagtt atatattgca cttattggaa ttttcaaccc ttcatattct tttattacca
     8581 gcaatccatc aaatttccct aaagacacta attgcgataa accgttatca acttcaaaga
     8641 ataccactgt ctcattatct tcactgtttc ctataataac ttttgatata tcgatatata
     8701 catccatgcc tgatgtatta atatttatct ttaaatcttc aatatctttt atacgagcag
     8761 catctgaaga tgataagact actgctatgt gaaatttttt ggaagttgcc acctttacag
     8821 cagttgatga ttccagtcga ttatcaccag acatttcatc atgcagatgt gttggataca
     8881 ctgatttcat ctgctcatat gtatgatatt gctgattata ttgcatattg tatgataaat
     8941 atccagagtt aactgcaact acatcaaata catcacttgc catagcctgc ttaagctgct
     9001 gctgctgaac atataactca tcaagttgct gttgtttttc attattaccg ctctttacat
     9061 cattgaggta ttcattaaga gcttctatac tctctttgtg tatttgaaca tctgaaagag
     9121 tctgactgga aagtactgat gaaaaggata aaagactctt tcttacctga ttatcaatta
     9181 ttttaatatc ttctgatatt attccagtat ttatctcata taactgctgt tttttctgta
     9241 tctggtcatt tatttcaacc agatcattat ataaaggatt gtttgtgctt accaaagaac
     9301 ctattaagct gttaactctt atcctgcttc cctcttcata tgtttttatt aacgagtatc
     9361 cctcaacagc atcatatgag tcatactcat aaagaaccag tcctgatata ttaatctgat
     9421 ctataagttt tccattatat gcaatatctt ttttcacaat ggaagagtag tagtttattg
     9481 ccattcttga tactagtatt acaaaaagaa aaaaagctat cactatcatc aatctgataa
     9541 tagcttttgt gttcttatcc tttttatcct ttttttcctt ctttttgcct tttatcatct
     9601 tttcagatat ttaatatgcc tttctttaac atcatatcac atgctttaag cacacctagt
     9661 tttacctgag tgtatgttag gcctccttgc atatatgcta tgtacggttc tcttaatggt
     9721 gcgtctgcag aaaattctat agatgctccc tgtataaatg tacctgcggc cataaccacc
     9781 tggtcatcat aacctggcat atcccaaggg tatggagtga catgcgaatc tataggagaa
     9841 cctgcctgaa cacctttaca gaactcaagc agttgttctt tgtttttgaa tataacaagc
     9901 tgtattatat cctgtctgtc ttctttatat tcaggatagc ttttaaatcc tagtttctcg
     9961 aaaagcagtg atgtgaatac tgctcctttt aggctttcca ttacagtata cggagccatg
    10021 tataatccct gaaacataag ccttttatta ttagtagctc cgacctcttt taataaccct
    10081 ggagcataaa gccttgccga cgcattcaag atgcactcct tagtgcctac tatatatcct
    10141 ccgcttctgc acagccctcc tccgggattt ttgattaatg aacctgcagc catatccatt
    10201 ccccaatgtg taggttcttc atcacataca aattccccat aacagttatc tacaaaaagg
    10261 attatatctt ttctaacagc cttaaccttt tttgaaatcg aaatgaactg ctcttttgag
    10321 acaggaggac gcaaactgta tcctctggat ctttgtatga atatcatttt tgttttatca
    10381 gaaatcgcat tcataactgc agtttcatct atcttgtcct caatatgttc aattattctg
    10441 taagttattc catactcggc aagactgcca gttgcctttg taatacctac agtacttctc
    10501 aatgtatcat atggcatacc atttgcaaaa attatctcat caccaggtct tacatttgat
    10561 gacatagcaa gactaatagc tgaagtacca gatgctatac tctcacgaac taatgcatct
    10621 tctgtattaa atatgtctgc ataaacttca tcaagaagat ctcttcctct gtctgagtaa
    10681 ccatatcctg tcgtttctgc aaaataggca tcacttaccc ttctgtcctg aaaaaccttt
    10741 gttatcctca cctggttagt ttctgcagta tcagaaagtt ccttgaattg attatcgcat
    10801 agctgttgca catcatcagc aagactgata agttttttat ctatatcaaa cccttttatc
    10861 attctaatat tccttttata tattttatcg catcagatat ctttattact gtctgtccat
    10921 ttacaggtgt aaactgatag gaattatgtt cctgatttat ttcaaatctc aagctatagt
    10981 cacagactgc atcagcaatt gatttaaatg gaagtataat atcatcctca ctacaactgt
    11041 agctaacatt ttccttggta acagttaatc tgcattgagg atatattttt atgacttttc
    11101 cgtaactgtc ttttacactt aaagaacctt caaattccat cttaaaatca tctttttgaa
    11161 tctcttttgc aagatatgtg ttctgccatt ttctccaggc tatcatgtct tggtaaacta
    11221 tatcatcttc agactgtgga tttatcaaac catatctgtc aataaccgct gtattatcac
    11281 aatcagtaca gtatagaagg ttcttctttg tatgcattga aaagtcctgt aggcattttg
    11341 ggcatttatg taatatcgca tgcattcctt ctgccattga cctgctatga aattttatgt
    11401 gttttcttct ttgccactcg tactcattat agctaagagc aattcttatt ttactgtcta
    11461 tatcatcaat actcatgctg cgcacttgct gatttgtaaa tagtacgaaa gacttatggt
    11521 ctatacgtcc tcttctaaac cctcttgaac atcccctata ccatcttgga aggctgattg
    11581 atgctccgtt aagatgcgat attactacat ttgagttgct ttgctttata agcttggcag
    11641 cactgttatt aaagggcagc attgacccgt caattgtcct agcgccttca ggaaacaaac
    11701 ctatagtgcc atttctcttc aatacattga tagccatttt tgtagaacgg atatcgctca
    11761 tgccctgtct ttttggtatt gcaccaaaat atttcagtat tttcttcaaa ataggatatt
    11821 caaaatatga tgaagaagta aggtaatgaa cccatttctt cgttacaaat gctcccatta
    11881 caaatgggtc gatagctgat acatggttac ctaatagtat tgtagaaccc tcaaggttat
    11941 caatgtcaga tgatttattt gatctgagat ttaaaacaat acggaatcct ccctttatta
    12001 tgccccttaa aagcacttta gcaactatgt tcggttcatt tatgtaatat gtatcttctt
    12061 gttctttttt ttgaatagta ttcaatatgc acatcctaat ctttagtgta tagaatttta
    12121 tcacttaacc ctgatgttag caatatatgt aaaactttga gtgctataaa gttgactgac
    12181 ttgcttgata aataatcttg ctttatttag aataggatga tgaagaatag taatgatgtc
    12241 tatatagaac ttatagtatc ccaacaaagc cataaaagaa gactttcctc ttttttaaga
    12301 aaagaaatgg gcatatctgg caactgcata agaagagcta aaaacttcga taatatttat
    12361 gttgacaaaa aacctcagca tactgactac attttatccg aaggccaact agtcactatt
    12421 gtagtcccag acaaagacac taaatctgtt atattaagtt cggatatgaa tcttaatata
    12481 ttgtatgatg atgagtacct tatagctgtt gacaaaccct atggtgtatt gtgccatcct
    12541 ataatgagtg ttgatgagga tactattgcc aatggactta agcatatcta ccctggcatg
    12601 ggtatacatc caatcagtag gctggacaga gatacgacag gaatagttat gtatgccaaa
    12661 aactctatag ttcatcataa aatgtctctt attgatataa ataagaaata tataggaatg
    12721 gtactaaaca gcccgcctgt tactgaaggc actatagaca tgccaattgc aagagtacct
    12781 gtttctacca tgctaagaca attcgatcca tctggcaaag aatccattac ccattactcc
    12841 acgatatttt ccaagaaaag cgcttccatc ctcctattta ctttagaaac aggcagaacg
    12901 catcaaataa gagtgcattg caaagaaggc ggtttcccaa taattcatga tactctttat
    12961 gggaaacctt ctgattatga ccagtatata tccagacaag cgttgcactc atatgaaact
    13021 tcttttatac atccttttac aaatgagaaa atatgcataa aatctgatct tcatgatgat
    13081 atgcttaatc ttgaaaaggc tatcagagaa aaataacttg tttatgaatc gttaaggtga
    13141 tatgcttttt ttaatggaat atttaaaagc aaaaggcatt gtgtgtaaaa tatctgatta
    13201 caaggaatct gacaaaattc ttaccttatt aacatacgaa aaaggaaaga tacaggttat
    13261 agccaaagga gtaaaaaaga aaggtgcagc tcttgcacat gcagcaaggc ttttctactg
    13321 cggcaatttt gaatttatct gttccaataa tattcccatt ttaacaggag catctcaaat
    13381 tcaggatttc tttggtctta cgcacaaaat ggaaacttta tattatgcat cacatttcat
    13441 gaatctatgc tcatatttca ttcaggaaga acagccatca catgatttaa tgtctcttct
    13501 tcttaacagc ttataccttc ttatgagaga ggaagcagat cctatgctat taacatctat
    13561 aatacagttc cgtattgctt ccttggaagg atctgctcca ataacagact tttgtgtaga
    13621 atgtcagaag cctttggata atgattgttc attctgcttt tctattgata atgatggaat
    13681 atcctgctgt aatgacggta catgtatatc tcatgcgatt tttaaagcca taaaccatat
    13741 ttgtaatgca gacgcatcca aaatatattt ctttaatatt ccaaaagctg atttacctgt
    13801 tttatatgaa ttgtcctgca gatatattga aaaaatagcc ggcaagcact ttgagatact
    13861 tgattggata aaagagttgt aattatattc aaaaattatc acatatatta tttataacca
    13921 agagtattca gcatacgatt atcatttcgc cagtcatttt taaccttaac ccataatttt
    13981 aaatttatct taaaatcaaa tgtatgctcc atggattttc tggcctcagt tcctatcctt
    14041 ttaagcatag atccgctttt tcctataaca attcctttat gactgtcttt gtcacagtaa
    14101 atgacggctt ctatatcata caaatcctta tcttctcttt tcttgaataa ttcaatttca
    14161 acagctgttc catgcggaac ttcttctgat aaaagaaaca gcaatttttc ccttatgata
    14221 tcttctgcca tctgtctcat agtctggtca gtaatttcat cctcatcgta atacatagga
    14281 ccttcttcaa gataactcac aatcatctgt ttgagctgtt ctacattaaa atcttcctgc
    14341 gcgcttatag ccataatctg gtcaaacttt acttttttag aatactgttc tgtataccat
    14401 ctggcagtag gctgatcaat agtgtcgcac ttattgatta cacatatctt cggtatatca
    14461 atatttgaaa gaagctcaag aattattaca tcttcataag gaatttcatg ttgcttttta
    14521 ggtgctgtaa caaatactat tacatcagtt tcagtaaatg ttgattttgc tgcagttacc
    14581 atatattcgc ctaatcttgt tttcggctcg tgaattcctg gagtatctaa aaaaataatc
    14641 tgactggatt catcagtata tatagccttg atatttcttc ttgtagtctg gggtttgggt
    14701 gttacaatac taattctctg ctttgttatt gcattgatta aagtagattt gcctacatta
    14761 ggccttccta ttactgaaac gaatcccgat ttgaaagtca tattatctcc cttttatatc
    14821 atcataaaaa tcaaaagaat ttggaagcag ttcctttagt ttaaatgttt tccattctct
    14881 gtctttcccg cttacaaaaa caatagcatc cttactcata aattcactca ttacctgacg
    14941 gcatataccg caaggaaatg cataatcatt aatatcacct gttatggcaa tagctgatat
    15001 gtatttttca ttttcactca tagctttaac tatcgctgtt ctttctgcac atatagtagc
    15061 accatatgag gcattctcaa tatttgctcc ggtatatatt ttaccgctgc ttgccaacac
    15121 agcagctcct attttaattt tcgaataagg tgagtatgca ttatctatac tgttttgtgc
    15181 agctagtttt aatgcatcat atttatctgt catatcccaa ctcctttaaa atattctcct
    15241 gaaattcttg catcagattc tcctgtttct cgtcctgatg gtcataacct agaagatgca
    15301 gaagaccgtg gcttgctaaa aacacgcatt cccgaatttc agaatgtcca taactttcag
    15361 cctgagaaaa tactttgctt tcacaaataa gtatatctcc tagaaataat tcattatttt
    15421 cataatcaat gtcatattta tcgtaaacaa ttttaccatc tgaactatca gtcgcaggaa
    15481 aggaaagaac atcagttact gaatctatat ctctttgttc aaaatttatc tgttttatgt
    15541 attcatcatc tacaaattca ataaatactg acaaacacag attatcatca tcaattgaat
    15601 tagcctgaag tattttgtct aaaacctttt taacaggctc ctcgaactgc ttagtcctgt
    15661 catttagacc ttcaatatca attctcgtct tcattatctt ctttttcctc ttctttttct
    15721 tccaaagata aagtttttgc agccgcaata tccttgtcag tctgattagg atatttaacc
    15781 ctctgatgga aatatccagt aaatactttc ataaaagcaa acaatatctc atccagatct
    15841 ttcagtgtaa ggtcacactg atcaaactga ccatcatctc tttttgactt aattactttt
    15901 ctgattgttt tctctatttc aataatatta gtagtttcca ttgacctaat tgtcgcctca
    15961 caagaatcag ccagcattac acaagctgat tctttagttc tcggtttagg tccagaatat
    16021 ctgtaatcct cttccattat gggctcatct gatacgctct gagctttgtg ataaaaatac
    16081 tgaacctgtg tagtaccatg atgttcctgg attatatctc ttatagaagg aggcagtttg
    16141 gcttgtttag ctaaatctac accttcattg gtatgactgg taattatggt ggctgacaac
    16201 tgaggagtca tagtattatg tggatttcct gtttttgatg actgattctc tccaaagaat
    16261 gccggtctgt tcagttttcc cacatcatga taatatgctc caactcttgc taaaagacca
    16321 ttagcaccta ttgcttcggc agcttcctcc gctaaattac ctaccattaa tgaatgatga
    16381 taagttccag gagcttctat cataagcctc ttcagaagtg gtcttgtagg agaggctaat
    16441 tccataagct taaatggtgt aactatatta aagaatgctt cgaatactgg taataatccc
    16501 agacacaaca gtgctgcacc catagaagat ataaacaggg ctatcagctg ggtgtatggc
    16561 atattgattg ttatatcagt tgttataagc atatatgcca tatatatgac catatttatg
    16621 acacctagag caataccaga aatagctagc cttatccttg cagaagtatt tcttacaatg
    16681 tatatagtag caatagaacc taagagcaat attgtaagat aaactatatc acctccgaca
    16741 atatagcagt aaaatacagc cattggacag gaaaaaacat acgaggtgtt gtcagatatt
    16801 aaaatagcaa gcatcatagg caagatgaaa tatggaatat acatatagta ttcagatgga
    16861 acaaaaagtg ctggtatata taccataaag aaaagcaaca caagtataaa tatggatttg
    16921 ggctgtaatt tcatagtgtg tccgcagatt ttttttgatg ccagcgatgc aaggatacac
    16981 ataataaaca gcactgccgc tccaatatac atataaagtt catttgcaac tttaattact
    17041 cccatttccg atagagtttt aatatcatca ctttgaagct tatccccttt ttcatacaac
    17101 actgacccag caaaaacaac tactggggaa ttatctatta catcctgata aactctttct
    17161 ttttccataa gagtggcaat ctggtctatg cggctgttca ccttcacaaa atagttttta
    17221 aggatatcag taagctcatt cagattacta tcagtaactg actcatttac caaagttacg
    17281 aatttttcct gatactcaat taaattatct tcatgaatgt aatcatccat aaattcagca
    17341 aatttagaac ggataacagt cttatagctg tcatatgtcg tatcattcac atctcttaat
    17401 atagctcttg caagattatt aggtatatct actccaagtg aatagaatat actagtatat
    17461 tggtttatca atgaattaag agctgtattg tactgagggc ttgtcattga tagtccctta
    17521 acctgtcctc tgtatacatc agtaacatca aaaaatccta taatgttttc ataaagagca
    17581 acagaagccg tgctaatcct gacggcaatc ggctctactg cctgctttgc tgcttcagcc
    17641 tttgataatg ttgcttcctt atcgtagtag tcaaaagatg ctactatatc agtagacgca
    17701 acattatcac ttgtaatcat atgacctgtg tcactgactt tttgaaatat gcccaaaaca
    17761 gcaataatag ctgcaagaag aagtatgagc acattatata acctgttctt taataaatcc
    17821 cttttattaa ttttggatct actcattttt attcaccttt tttttgtttt ctgcccgttc
    17881 atatgcatta atgattttct gcacaagctc atgtcttact acatctttga aggtaaggtt
    17941 tattatacct atatcatcaa tgttcttcaa tatgtgtatg gctgttataa gaccggactt
    18001 tttctcacga ggcaagtcta tctgggttat gtcaccattt ataaccattt tggagccaaa
    18061 acccattcta gtaagaaaca ttttcatctg ttcttctgtg gtattttgag cttcatcaag
    18121 aattatgaaa gcatcatcta gcgtacggcc tctcatatat gctaaaggaa taatctcgat
    18181 taatcctttt tctttattcc ttgctacatt atccggaccc attaattcaa acagtgcatc
    18241 atatagcggt cttaaatacg ggtcaatttt agattgcaga tctccaggca agaatcctaa
    18301 tttttctcct gcttctactg ctggtcttgt aaggataatt ctttttacgc tctttgctct
    18361 aaatgcagtt acagcgcatg caactgctaa gaatgttttc cccgtacccg caggacctac
    18421 tccgaatgtt ataccgtttt tctgtatggc atcagtatac tttttctgtc caagcgtctt
    18481 tgcttttata ggtgctccgg atgcatttag gcagatatat ccagaaggga gataaaacga
    18541 ttcatctttc ccctggttaa tcaagtccgc taaataaagg atattctgtt tattgatatc
    18601 ctctcctgcc tgcgacatgt ctatgagtct tgtaataagc ttttcagctc tgtttacctc
    18661 ttctacctcg cctttcagtc taacctcgct tcctcttaca attacagata cattcatcat
    18721 gttctctata actttcaaat tagaatccga gcttccgaaa acgctcatca tatcacttag
    18781 attttcaaaa ttaataagtc tctccattaa ctccttcaca taaagcaaat tggcctatat
    18841 cgtcttgtca cataattata tcatttagtc ggtattttaa tcaaacaggt atttaattgt
    18901 caggcttgct tttaagttta gtatgtaatt gatgcattgc ataaactgtg ctggtagaaa
    18961 gatgatgctc tatctttgta gtctctgttt ctgctgtctg attatcaaca gataaaacat
    19021 cttttaggaa tttcaatata ataatatgtt tccctgttgc cgctttgact gcttcaatac
    19081 ctgcgtctgt aagaaatata ggctcatact ttagctgtac gcaatatccc attttagtca
    19141 gtttcataac cgcgttgttt gcagaagctt tctttacttt cattttttca gccagatcag
    19201 taagccgaac gcctttattg gcatatgata attcataaat gcttcttaag tagttctcca
    19261 tggattgtgt taatccgctc attttaccac cgccccgtat tttaaacaaa atgttagttt
    19321 tatcttacca cacaaatttg tccaatttca accgttatat aatttatttt agcgaaaata
    19381 atcatttatc aaataaacaa cacagatata catataatca gtatgcttat ttaaataaaa
    19441 tatatgttat acttgtcagt agtaaatgat ttgtttctac tattaaaact gtttaaaaga
    19501 ggatattata tgaaagttca tgaaagattt ttagattatg taagatttga cactacatca
    19561 gaccctaatt caaaaacatg tccaagcact ccaaatcaaa tggagtttgc cagatactta
    19621 gttgaagaca tgaagaaaat cggtatttca aatgcatatg ttgatgaatt tggttatgtc
    19681 tacggtttca tagatgcatc tgaaggtttt gagaacgtag aatcaatagg attcatagca
    19741 catatggata catctgatgc agttagcggc aaagatatta atccatgtat tatatctgat
    19801 tatactggtg atgatgttgt tctaaatgaa aaattaaata tcattatgga agtagataaa
    19861 tatcctcatt tgaaaaatta tacaggtcat gaccttatag ttacagatgg cactaccttg
    19921 ttaggtgtag atgacaaagc aggtatagca gaaatacttg aaatggctga cattcttata
    19981 aatgaccctt caaagaaaca tggcagaata tgtatagcat tcacgcctga cgaggaaata
    20041 ggaagaggtg cagacttgtt tgatgtcaaa aagttcaatg ctgcctacgc atacactgta
    20101 gatggcggac ctttgggaga tatacaatac gaaaatttca atgccgcttc aacaaatgtt
    20161 agaataaccg gattatcagt tcaccctggt ggcgcgaaga atataatgaa aaatgcaata
    20221 ttgatggctt atgaattcaa ttcactgctt cctcattgtg aaactccagc tcataccgaa
    20281 aaaagagaag ggttttatca tctacataat atatccggtt ctgaagaatc agcgcatatg
    20341 agttatatat taagagatca tgattatgaa aagcttcaac agaaaaagga ccgaatgaaa
    20401 ctagctgcag aatatattaa tagcatatat ggggaaaact ctgtaactct ggaaataagt
    20461 gatacatacc tgaacatggc agaaatgtta aaaccccata tgcatattat tgaaagagca
    20521 aacagggcat atgaagctct aggctataca cctacaactc atgcaatcag aggaggaact
    20581 gacggagctc gtttatctta catgggactt ttgtgtccta acatagctac cggcggacat
    20641 aacgcacatg gtagatttga atatgtatct attaatgaaa tgaataagat ggtttctgta
    20701 cttgttcata tggcctgtga tattaatcaa tagtaggatt ctgagttaaa tattcattta
    20761 cgaaagcatc cagatttcca tccattacag actggacatc accagtctca taattcgttc
    20821 tatgatcctt taccatagta tatggacaga aaacataaga ccgtatctga cttccccagc
    20881 ctatatccat ctgttcacct ttgagatctt caattttttc tttgtgctct ctttctttga
    20941 tttcctcaag cttggacatc agcattctca tagcagattc tttattctga tgttggcttc
    21001 tttctgactg acaggctact actatatttg ttggaagatg ggttatacgt attgctgaat
    21061 cagtcttgtt tacatgctga ccgccctttc cagatgagcg gaatgtgtct atccttaaat
    21121 cttcagtctt aattttaact tcaatatcat gggaaacttc cggtattacc tcacaagatg
    21181 caaaagaagt atgtcttctg cctgctgagt caaaaggaga aatacgaaca agtctgtgta
    21241 caccgctttc acctcttaaa taaccatatg catattcccc ttcaacataa acagtaacag
    21301 actttatacc tgccccatca ccatcaagat aatcagtaac agttgcttta aaacctttag
    21361 attcaaaata ccttgaatac atacgaaata gcatttctac ccagtcgcaa gcctctgttc
    21421 cacctgctcc tgaatgcaaa gtaaggtaag cattattctt gtcattagtt ccgttaagaa
    21481 gagaatacat gcgaatctgt atatatgcat tctttaaatc ttcctttgca gttacaagtt
    21541 cgttttccag ttcctggtcc tcaggatttt cttcagaaaa ttccaacaag acaaaacagt
    21601 cctcaagctt ggtttttaaa tctcccgctt ttgcagatct gtccttaagc actttaatcc
    21661 tctgaagtac tttctgtgaa ttttcattat cctgccagaa agcttcctgc atagtctgat
    21721 gttcaaggtt ttctatttca ttcctagatt gttcagaatc caagcttttt atgagctttg
    21781 ctgattctgc tttaatatca tttaactcgt ttcttattgc tccgtaatca atcatttaag
    21841 cgttttcata aacctttcta tacgtttcaa agcttctttt atattctcaa gcgaacttgc
    21901 atatgtagct cggaaaaagc cttctcctgc atcagagaat gctgttcctg gaactacaag
    21961 cactttgtgt tccattagaa gcctgtcaca aaactgttct gatgtaagac ctgtttttct
    22021 tatattagga aatacgtaaa atgctccttt tggctcaaaa caatctatac cgatattctt
    22081 aaggccatta tagatatatc ttcttcttgc atcatactcg tttcgcatat ttataacatc
    22141 accatcacat tgctttaaag catcaattgc agcatactgc acgaaagatg gtgcgcacat
    22201 tatagcatat tgatgaattt ttatcatctg tgaaataata tctctatgag cacacacata
    22261 tccaagcctc caacctgtca tagaataagc ttttgaaaat ccgtttacat atatgacttt
    22321 atcatgcata ttttcaaatt ctgcaaaaga tgtatgtttc attccatatg taagttcagc
    22381 ataaatttca tcagagatta ccattatatc cttttttgat aagtaatctg caataggtgc
    22441 aagttcctct ttagtcatta cggcacctgt aggattgtta ggatatgcaa gtataattgc
    22501 ttttgtcttg tctgttatgc atttttccag ctgatctaca gtaagtttaa agcagttatc
    22561 ttcactaagc tgtagtggta ctgcttttgc tcctgtgaaa ttcacacaac ctttgtaagc
    22621 tacaaaacac ggttgtggta ttattacttc atcaccacaa cccgcaatgc ttcttatcgc
    22681 tatatctata gcttcggaag cgcccacagt aataattatc tggtctttcg gatcataatt
    22741 taaattatat ttctgatgca gcattaaact caatgcttca cgtaattgta aaataccatt
    22801 atttgatgtg taatgggttc tgcctttttc caatgcatgt atagctgact ctcttatctg
    22861 ccatggtgtt gtaaaatccg gttctccaac ccctaaggat atgacatcat ccataccatg
    22921 cagcatatca aaatattttc ttatacctga tggcggagtc ttttccactt cgcttcttat
    22981 aaaatcttta ggttttttca caatactata gcctctctct gatccttttc ctcttcttca
    23041 aaaacaagct cattctcctt gtatttttta agtacaaaat gagttgatgt tgaagttacc
    23101 cctgatatcg tagaaagttt ctgagttaca aaaagtgcta cttctttcaa atcctttccc
    23161 tctactattg ctgctatatc aaaagaacca gacataagat aacatgtttt tacctgctta
    23221 taactgtata ttctttttgc tatttcttca aaccctctgt ctctttccgg agcaaccctt
    23281 atctcaataa aagcaattac tatatcatct tttgcttttt cccagttgat tactgcagag
    23341 taccctgcaa taactttatc ctgttcatat tgcttaatga ctctttcaac ttcttcaact
    23401 gtttttcctg tcataactgc aagttcctga gcagttaatc ttgcatcttg ttttaacaat
    23461 tctagtatat ccatttatgt ttccttcctt atacaaaaaa ggagaacgct tggtcctcct
    23521 gaaattgatt tttgtgttga gttatttatc ctcttcagta gaacctaaag ttttttccag
    23581 agaatcacta aatttctcca gaagctttgc cgcattagct tgtaatgttg ccaattgttt
    23641 tttaagatca actatttttt cacgttcttc ttctgcctta atctctaact cagttacttc
    23701 cttaaaagct gactcttttg cctcttttac aatctggtca gatagttcat tggctttgaa
    23761 taaagctttg gttatgttat ctttctcttt atgaagagta tcttctctgc cttttaattc
    23821 ctcgtacttt ttctgaacat catcaatagt ttcctgcata ctattgataa ctgtatcttt
    23881 ctgagctatt tttgcttcat actcttccat cattttttct atatatttgt ttacttcatc
    23941 cttgttgtaa ccgctcatcg agctacggaa tcttttcttt gccatatcac acctcttctt
    24001 agtattgtct gtttactaat tttatactac aaatcactta aatatcaagt ttttttaaaa
    24061 tatcatgctt tctttgtgtt cttttatcca tttctcagct gctttaaccc atatttcgtt
    24121 aggtataggg tcataattct cataacctcc atcacgggcc atattttgcg ctttatgaag
    24181 aaaatccaga ctgtgataga atttcgtata aaatgaatct gcaataagct ttatccagaa
    24241 atcgtgctta tacttatcta tatctttcaa gtagtatgat gataactcat caggatcata
    24301 ttggatgtaa acattctcgt aatcaaagtt ttttgtgttt tcatctacag atatgatacc
    24361 tatgctgaac gcatcatcac ggttatagca tttcatagca caaggtatac ccaaaggtcc
    24421 gggattgatg aaatgcttgt ctttaatttt caagcagtat gactgatgag catgtcccag
    24481 taaaaacaaa gaacaatcat atctgtcgta catatccatg aatgtatcta tgtgcgacga
    24541 aggtaatatg aaatgccttg tacttatatg agacccatgc accataagtg tgtcaatacc
    24601 aaacagacta attcgtttta catttggaag acttgaaata tatcccaaac caacatcatt
    24661 gaggttttca tatgttttaa ccaggctttc gcattgatgg ttatcatccc agatatctct
    24721 ttcaccttta tgatagctaa tcatatactc ttccctgttt cctcttatta catcatctgt
    24781 aagattagat ataatatcaa gaactgctaa agaatccggt ccgtcagtta ccatatctcc
    24841 taagaaaata tacctctcca ctccttgatc tttacatata tcaagagctt tgagaagcat
    24901 atgaagattt ccgtgtatat ctgaaaggat agcggctttt ctcatttatg taatgtgctt
    24961 tctcgctatc ttgtccattt cctcttcgtt ctttggaggt tttacatcgc tgaaaagttc
    25021 ccattgttca ttatgttcga ttttctcttt tgggcctaat aacataagtg gtgataaact
    25081 ttccatctct gtcatgaaat cacatgtata tgtctcgtac attgaagcgg aataatcagg
    25141 ataaaccatg tcttctaatg gagagaactt ttttacgaac atatcatcat tattaaaata
    25201 tgcagcccac ccgttagtat tagaaattcc aaatttaaaa ggttttatgg cttttgaatc
    25261 gtgctgtaaa aatatatatc tactacccca ctggactctt ttatctgaca tatctgtgta
    25321 tggccataaa gaaagcattc tgttaggaag aagcaatgta tccagtgttt cctgaggaac
    25381 tacttcaagt ccacctggtg ccaccacagt aagagcccat gctgcaagtt caacatccca
    25441 aagattatag tttgtcaatg tatgcttaag ataaactttt gaactttcat catcaagaac
    25501 tacttcaata tcttttttta tcatagttgt tttttctaca ggctgtgaaa ctttaatgcc
    25561 attttcaata atctgtacat ctactggaaa gttatcagga taataacttc taatctcgtg
    25621 ttccggactg tgccataatc tgtggcctcc atatatcatc cattcatccc cacctgattt
    25681 tcccatttgg tcttcttttt cacacataac gttaaatctt cctataaatc catatcttat
    25741 tatccttgga cctacatcag aagtaacaac gacttcaaca gtattattaa atattctata
    25801 gcaattattc catcctccat acgggatttt ttccactttg actgtcataa aacacctccg
    25861 gcttttttat aataccatta tactatgcaa ttaactgcat aggtgattca ttagaattta
    25921 attctgtatt ttatcctttt ttacctgtaa accagtggat tattgggata aataggaaaa
    25981 ttatccagcc tggatgccat agattccaaa taaatcctat tgcaagataa ataagaacta
    26041 ttatcagcgt atatgatact gctctggact ttattgcatt aactaccgca taatacactg
    26101 gtatagtaag gaacactatc cagccaggat gccatagatc ccatataaag cccattagta
    26161 agaaaactat tgttgcaagt actggatacg gaaatgtgtt aggattcctt ctgttatacc
    26221 atttatcgat atttatatcc actgtataac cttttttctt ctcattttcc tgtttgtttt
    26281 caaaatcgtc catttgtgcc cctttcaaat taagtgaaat aatgaaatga tatggatatt
    26341 gtaatataaa atggtaaaaa tatcaaaaat ctcttagtat taagtattta aattcatata
    26401 ctgcaataat tctactcgta catattattg tctattttta agtgaaagaa aaagcctaaa
    26461 aggctttttt tgaatttttg cttatagaat tatgaaatac ttaccttata ctatttatca
    26521 aagttatatc ttatagtctt ctcctgtgcc ataatacttc tcaacataat caatatcctt
    26581 atctcctctt ccacttaagt tgaccagtat tgaattagct gtattggctc ttgcagcttt
    26641 aatagcataa gcaagcgcat gagaactttc aagagctggt ataattcctt cgtatcttga
    26701 aagcatgaag aaagcctcta tagcttcttt atcggttact gatgtgtaat caactctttc
    26761 aatatcatgc aagaatgcgt gttccggacc tactgatgga taatctagtc cgcttgctat
    26821 tgaatataca ggaagtggat ttcctttgtc atcctgaaga agaaggctct caaagccatg
    26881 taaaattcct tttttaccga atgtaatgct tgcagcgtta tctccaacat taggccctct
    26941 gcccattggt tctacaccta ctatctttac aggatccttc aaaaatgcag taaacagtcc
    27001 cattgcattg cttcctcctc cgacacatgc acaaatctgg tcaggaagct ctccagtcat
    27061 ctccatatat tgctctcttg cttctattcc tactaccatc tgaaaatccc taaccatcat
    27121 tgggaagggg tgaggtccta ctactgagcc tatacaataa atggaatctt tgtaattctt
    27181 catatatgat tcaaaagcag aatcaactgc ttcttttaat gtttttagcc cgtgtgatac
    27241 aggtactact ttagccccca atatcttcat tcttgtaacg ttaggtgctt gtttaattat
    27301 atcaacttca cccatatgaa tctcgcactc aagtccgaag aatgcagcag ctgttgcaag
    27361 tgcaacgccg tgctggcctg ctcctgtttc tgctataaga cgttttttac ccatatattt
    27421 agcaagtagt ccttcaccca tacagtgatt cagcttgtga gctccagtat gattgagatc
    27481 ctctctcttt aggtaaatct gacatctgcc aagcctgtta gataatctct cgcaatgata
    27541 aacaggtgta ggtcttcctt gaaattctct tcttatacgc ctaagctcat tgataaatga
    27601 ggctgaatga gcaattgtca tatatgcctg ctcaatttcc ttaaatgcat caactaactc
    27661 aggcggaaga aatgctccgc cgtattgccc aaatcttcct gctatatcag gataaatttt
    27721 cgtgtagttc gtaaaatcca tattaccgtc ctccaattta atctactttt tatttagcat
    27781 agctttcctg taggcttcgc cttcccaacc cattatgaat gctatatctt catccttcat
    27841 agtatatgtc tcaagattat ttcttttggc ttcatccatg ataaacaaaa aagcacatat
    27901 actctcttcc agagtaagag cttctttgta tgagacataa tactttgtta taccgcttga
    27961 taaatcaatt ctggcttttt tctctgcgtt ttttgttgta acctcaccaa gaagatacgc
    28021 tgcctggtca ggatatagta tatttgaatc gaaccattca tcagtatatt ctttaccttt
    28081 aaggtaagct ataagccttg gagtattaga aacatatact ccttctgatt ccatcttcac
    28141 tgatacatct ccatataatt cagtaatatt taattttttt ctgatacttt gagaaacata
    28201 atcattaatc ttttctgctt catccaaagt ctttgcagtt attataactc catggcttcc
    28261 catgaatata atattaggta tacatcccat cagttcaatc tgcttttgta tttccaaagt
    28321 cagataaaag ccaggtgcta tatatggaag ccataagtac ctatactgcg aattttcaaa
    28381 aagctctttt gctatacttt caccttcttt tgtacaacaa agaagattac cgtatactga
    28441 atgagtatgt ataacatatt tttccaacag agaatgaaat cctgcttcta ttgaaggtct
    28501 tccttcattc aagccttcaa gcttaaccat attatccttt acaaactgtg agctttcttt
    28561 atagaagtct cttttttcag ttaagtctac tgttttatag tattgtcgaa cttttgcata
    28621 atcaacaaca acaaatccat ctttttctgt tatatcgcaa agtttacatc ctgaagcttt
    28681 tactgccata agcttatcat caaatttaac agatgtattt ccaccaccgc cttgtgtata
    28741 atccggcatt tgtcctactc ggcaggacat ttctatcatt tccttaataa atctatcata
    28801 cattttggac acctcttttt cgcttataaa ttggatttta tacgcatttt aacctttatt
    28861 caatatattt tttttgtaat attcatattt ttcttcccaa aggtctaaat ctttaggcaa
    28921 atatgattta acttcaaaac tgttcttgac tatttcgcgg gcttctttga gattttttat
    28981 ttcctttaag gctataagct gagcaacaat atttccaatt gcagtagcct caataggccc
    29041 tgatgttact tcaataccta aggcatttga agtaaactga gatatcatag tgtttttaca
    29101 tccaccgcct accatatgca gtttggggat acgacttcca actatctcac ataatccatc
    29161 aaatacatat ctgtatttaa gtgctaaact ttccattatg catcttacga tttctgctct
    29221 gctttgagga acatactgcc cggttcgttt gcaaaaatcc accactcttt ttggcatttc
    29281 acctggatgg taaaaatcca gatgatcaac atcaataaga catgcaaaag gttttacttt
    29341 tctagccgac atttccaact catcaaatga aaccgggtca ccttctttat tccactgtcg
    29401 tctgcattct tgatatatcc ataagcccat aatgtttttt atcagccttg ctgtcctgtt
    29461 tatgccacct tcatttgaat actcatattt atatgttaaa tcatttacta ccggactgct
    29521 aagttcggaa cctaatattg accaagtccc acttgataaa aatgcaaaca aatccgtatc
    29581 agctggtgct gatatcatag ccgaaccggt atcatgacct gcaacagcag ttaccgttat
    29641 cggttttaat ccagtttcat aacaaactga aggagtaagc gtgcctatta ctgttgcact
    29701 gtcttcaact ttttctaaaa tattagttgg aattttaagc ttgcttagta gttcaaagtc
    29761 ccagtcacca gttttggagt taagcatctg tgtggtactg gcaatagaaa attctgatat
    29821 tttctttcca gtcagaaaat atgataaaag atcaggcata aataaaagtt tatccgccct
    29881 ttccaataac tctggattat tttttgctat agcataaagc tgatagattg aattaaactg
    29941 cataaattgt ataccggttc tttcgtacaa attacgttta ccgattatat cccatacttc
    30001 atccattaat ccatcagtcc tcttatctct gtaatgtaca gggttttcta gaagtcttcc
    30061 cctttcatca ataagtccga aatcaactcc ccatgtatca atacctatac tttgtatatc
    30121 ttcatggcct gcttttttag cttctataag accagtcttc atttcataaa aaagacgtaa
    30181 tacatcccag tacagggtat catgtacata aacgggatca ttggaaaatc tatgtatttc
    30241 ctcaatattt attttttttc catcatatgt ccctaacata gctctgccgc tgctcgcgcc
    30301 aaaatcatat gccaatacct taatcatttc atacctccaa tgatttttat tgattcacta
    30361 ttaaataaaa attatgctaa gtctattttc tcaatatact tatcaagctg agcattctca
    30421 aattcagcag ccagttttga caaaggttct aactggtctt ctaaagcaaa tagctcgaaa
    30481 gcctttacat actctttata tctttcttta ggtatgtata ttggcaggaa tccttctttc
    30541 ataagaacat aattcattat cagtttgcct gttctgctgt taccatctac aaatggatgt
    30601 attttcataa acatagcata tgcatacatt gctttttcag tagctgtctc ataaggaagg
    30661 taaggaagtt cgttaaagta cttcttcatt ttatagtatg ccttttccgg tgatggtggc
    30721 acatgctttc ttccaggtat cagaagattt acatttctgt atactccgcc tactgatatg
    30781 tcctcaacaa caatagcatt aatctctttt actgcttctt catcaagata tgtattcttt
    30841 ttcaaataca tcttcatgaa acgaaaagcc ttgctatggt ctgtaatctc gagtacatcg
    30901 ttatctttct ccatgtcttt tgcattgttt tccaaaatgc ttctgacttc atctctggaa
    30961 agcctgtttc ctttgatttt agtagattca taggtatagc tgcactcgaa ttctttttcc
    31021 agagcctcaa tctgctctgt ggataacttg gacaaattac tgttcacata atctcttttc
    31081 ctgcacaatt cttcgtagct caattgcaaa caccttcttt cctatttatt ataaaacata
    31141 gctttggtta tgtccttagt cgcttcctgt tcatgcttaa ttaaagcata cgtggattct
    31201 ctcaaattgt caaaaaatga atccctcatt gccttacata cgaaattctg ccttggcgag
    31261 ttaatgtctt ctccacttat ctgaaataat tgatattgct cacataatgc ttttaccctg
    31321 acaagctgct ctttggtatt tcttgaaggc atataggtaa cagcattaaa accaagttct
    31381 tttaaaatat agaagagttt atctatgtat gagtcttcaa acttctgagg ttttttatct
    31441 cctgtaaccg aattttttac atcacctaag tatgcataag cagatattgc ttttgtctta
    31501 ttgcataaag caattatatc ttttacatcc ggacactcat catatgcgtc aacatagaat
    31561 aaagatataa gatcactttt aatcgcacct aaaagatcaa attcatatac cgggttattc
    31621 tcgtcactta atagattttc attcttctcg ctaagaggca gtttcaatgt ctgtttataa
    31681 aaagacacaa gcgctttccc tttttggaaa aggtctatca tcttaagcgc tagcgcatag
    31741 gatatatgcc tttcggtaac tgaaccacct tgctgataca aagataacgg aagtacatct
    31801 ttttcgaaat ctataaagat gtcatattta ccgaaaagat catttatctt attacacatc
    31861 atctgatttc ttttatttct ggcttctctg tatggcgcca tgaaatcctg tattgctttt
    31921 atgtgctggt gaggtatccc atgcagagct acataggcta ctgaatcctg atctgggtta
    31981 tttatcctta taccattcaa ctccgtcttg tccatacgca ccctaacttc aattccgcat
    32041 gttatcggca tatcaagtat tgcaccagcc tgtatgaact cttcacatcc tgcagcagaa
    32101 tcatgatcca taatccctgc tgttttaagg ccgttcatat atgccataaa aagtgcttta
    32161 gtgggactat atggtgaaaa tgaataattt gtatgaatgt gattatttac atcgttaaaa
    32221 aataagggct tttgaatatc acccttatca atggcatttt tcagcactct taaactattc
    32281 agtctttctt cgctcttagg attattaaga ttttcaattt gtaaaagaaa gtattgattc
    32341 atatttaacg catttcctgt ccaccggtta catttatagc ctgtccagtc atatagcttg
    32401 acatctcaga agctaaaaag accattacat tggctacatc ctcatattca catgatctgc
    32461 ccattggaac ctgatcaagg tatttctgtc tgacttcctg ttcagttatg ccctgattag
    32521 cagcatactg tttaaataaa ctatttaccc atagtgggga attcaacaaa tttcccggac
    32581 atatggcatt aaaccttata ttattcttag catattcaag accaagactc tgtgtcagtc
    32641 ctattccacc aaattttgaa gccgcatagg cactatttct gaaagaacct tttttgcctg
    32701 atttgctgtt tatctgtata aatgatgctc cattttcatt attcagcata tgttttactg
    32761 ctgatttagc gcatatgaag tatccccata aatttacatc ctgtaccagt ctccatttat
    32821 ttacatcaaa atcatgagct gaaccggata ttactatacc tgcattgttt acaaaaacat
    32881 caattcttcc gaatttatca atagctaatt gagtcattgc atcaacttct tcttcttttg
    32941 ttacatcaac tttacatgct actgcatttt ttaatccatt tgcaacttct aatgcttttt
    33001 cataattcaa atcagccaca acaacagaac atccttcttt gtccagtctt tgtgctaatg
    33061 cttcgccgag accttgtgct gcacctgtga taacggcgac cttattcttt aacatttcca
    33121 tatattgctc ccaacaaatt attctttata ttattatatc ccaatagttc ataaaaacat
    33181 atagggttgt gtgtttttcc atactttacc tgaaaaaata ccagtaaatc agcaatgctg
    33241 atatagctaa aattattagt gcaatcacta tagtctttat tactttgctt ttgtattcgc
    33301 tgtctctttc cttcatacta tttccctcag tttattcatt attttcacca tagcatatgt
    33361 atctagtttg caatattcaa gcaactgttt tctgactgtc tgtttctctt cttcagataa
    33421 caagggcata tttttaaaag ccgtcattgc ctctccaccc tttcttactc cttcaagatt
    33481 attgtaattc atggcatcat cttcaggaaa aagagcaggc aggacatact taatggaata
    33541 actgccctgc atagctttat cataatacca gtgattctta aaaggaacca ttaaatccac
    33601 catattattg tatatatcca ttaaggcagt atagtgttga ggatactgat atgcaagatc
    33661 cgcaataatc cctttttcaa aagccatatt gtatgctaaa gaacaggatt gtcttggtat
    33721 atcgtttata aggttctcag ctaattcttt tctgcagtca aaaccatcct tagctaaata
    33781 ttctttatgc tcaacagtac catcttcttt ctctatatgt aaagaatatt gaaagggcat
    33841 ctgctgatat ggtttcattc catcatatgg aggtatggca tattgtacag tttcaaaatc
    33901 aagataatat ataggataat ataaattagc aagaaacctc aatatctgct tcttatcgat
    33961 tacaagtttt tcctgtaact gatcttttat aacaggtctt aatttggcat atatttcttt
    34021 actgtaaatt acatcatcga atgaatatat cccactatga tacatcataa gcttattctt
    34081 tttgttaaga ttccttatat caaatatatt ctgctctggc agatgtcttg tgcaataccc
    34141 aaaatatccg caaggatacg gtgaaaaaca atgctcggca atatttattt ccggttcttt
    34201 ttccaaatcc aggtcctgtg ataaaaaatc taataattcc tcaactttgc tatgcctgga
    34261 ataagcctgt tcagtaacat cattaataga aaacagttta cttaaatcta aattaccctg
    34321 tcttgtatat tctgaattaa gaataataac atttgctttt ctgacattca ttccaagaag
    34381 attaagaata taactttggt aagccacatc atcaatatac acatctttta cactggttga
    34441 actcttaact tcattaataa ccactccatc ttcataaatc tctaaaatat caacactcaa
    34501 aaaacggccc atatatgaaa aggatgcttc agcaatgtat ttgcttttat tatctataaa
    34561 ctgttttgtc tgatttatca taatctgctt gtcagtctca aaagcaactt ttgatatatc
    34621 tggaaaaata cttaaagcaa gttcgcctac atcattacca ttttccaaaa cagcttcatc
    34681 taaaactgaa ttgtcaaact cttgtggttt gactctgtcc atccagtaca ttttcaagca
    34741 ctgaacagct cttatatatc tggattttga aacattgaat tctatcatat cttagccttt
    34801 attatttctt cgtcatattc tgcaacagca cagttatcta atatatagtc atgtactaca
    34861 ccattttgtg gaactccttt aaagtagtgc cctactgcaa tagacacccc tccatgtgca
    34921 actaataata tattcttatc ataatattta tttaatatat catctaaaaa tcctgatatc
    34981 ctttcataaa acaaaggata cgcctcaact gtatctagtt gctcctccat cttaagagac
    35041 catgctcctt ttcggtattc catatttttc ctgtatgaaa ttccttcaaa ctttccaaag
    35101 tccctctctt taagtctttc atctatcaat atctcattat ctcgatcttc acatactata
    35161 ttagctgtat gtacagctct gccaagcgga gatgatatca caacatcaaa ttcataatta
    35221 tctaaaatct tttttgtctt gtaagcctgc tgtatgcctg tttcattaag tggtatatcc
    35281 gatgatccct gcactttatc gttaatgttc caatcggtct gaccgtgtct gattagtaaa
    35341 attctcatat gattatgata acataaataa acctctttgt cttgaaatca acctgtcaaa
    35401 gaacattaac aatttcataa gtttcaaaaa aaaagacctc gccattgaga tcttcaaaat
    35461 aatactattg acagtactta tcctgtaatg cgtcaaatcc tcgttccccc gtccttatct
    35521 taacaacatc ctctatatct gatatgaaga ttttgccgtc accaagtgct ccggtgttaa
    35581 gtacagtctt tgctgcttct attacttttt caactggtac tgcacttata actacatcaa
    35641 ccttaagttt cggaaccagc gttatatcca atggcgcacc cctgtaagtc tctgtatgtc
    35701 cgttctgcat tccacatccc attacttctg taacagtcat tcctgtaacc cctatcttat
    35761 ttaaagcata ttttaaagct tcaaattttg gttttttaag tattatagat actaaagtca
    35821 tctttggctg cagtttatca ttaacagttt tcccactaac atgcttcatc tgagcaggtg
    35881 ttgtttctga tgggtgatat tctatgacat tcaacttgtc tatatccagt tcattattta
    35941 ataaaaagcc tgagtatgct gttgcaagat tatgctcaga aaagtccagt cccccgattt
    36001 cctcttcttt tgaaactctt aatccgacag ttttcttaat tatcaggaac atagcagtca
    36061 ttgttatagc tacccaaaga gcaacagctg caactccaag cacctgaatg cttaaaaacc
    36121 tgaaagcatc agcaaatgaa gtgttacctt taaaagcacc aactatccct tcgttaaaag
    36181 aaaatatacc taccataaca gtacccaatg ctccattgaa accatgtact gcaattgcac
    36241 caactgggtc atcaatcttt gcctttttct ctaaaaactc acatccatat acaactacga
    36301 accctgagat aaccccgatt atcaaagcag ccattggtga aactgtatca gttcctgcag
    36361 ttatagctac aagtcctgct aatgctccat tgaaagtcat tgaaacatca ggctttttat
    36421 acttcttcca ggtaataata agacaagtta ctgctgcagc ggcaggtgcc atgttcgttg
    36481 taacgaatat tagactaagt attccttcct gacccgttac cccgtatgtt gatgctccat
    36541 tgaatccaaa ccagcaaaac cataaaatga acactccaag agctgcaaac gtaaggttgt
    36601 gaccaggtat tgcttttggt tttccgtctt tgtcatattt tcctattcta ggtccgatta
    36661 tttttgctcc tataagtgct gatatcccac caaccatgtg aacagcagtg gacccagcaa
    36721 aatcatgaaa ccctaactga ctcagccagc cgccgcccca tatccagtga cctgaaatgg
    36781 gataaacaat catacttata attactgaat atatgcagta actggaaaac ttagttcttt
    36841 cagccatagc tccagatact atggtagccg ccgtagcaca gaaaaccgtc tggaatataa
    36901 gggaagtcat atctatgttt ccagtaataa agcctgttgt aggcaaccct attattcctg
    36961 caatatcatc accatgcatt aatgaataac ctaagaccca gaatactata gttccaagac
    37021 agaaatccat caggttcttc attattatgt ttccagtatt cttagctctt gtaagacctg
    37081 cttccactat tgcaaatcca gcctgcataa agaataccaa tgcagctcct gttattaccc
    37141 atacaagatc tgctcctgtt aatccactat caattcccat ttaattctcc tccttttttt
    37201 acataagaaa aagcgctgta cctattcagg ttcagcgcct ttgctaattt tttcacatta
    37261 tattccatca ataagtgtat gtcaatggaa atttcgaaat ttcatacttg cttaagtctt
    37321 aatttaagtt tttatacttg ttatatagtc aatcatataa caagaacatt attttctgaa
    37381 cacttgtcca gcaccttttg ttcaggcata gtatcgcata cccatatatc gatatcctta
    37441 agctcacaga atgtatatgc cttatccctg tcaattttag atgaatctac aaccataata
    37501 gtttttctgg ctttttccac tatacgggtc ttcaatttcg catcatacat gctggacacg
    37561 gaaaaacctc tgtcttcgga aaatcccgat gctcccatta cagccatatc aatattcatg
    37621 gtttccagca tctgcattgc ttcaggtccg gaacaagcta cagtattagg atttacggta
    37681 ccgccaagta ttataatcga attatcagta ttggacagtt tcaatgctgt attaactgct
    37741 gatgtgataa acgtacagcc tttcatatcg gtcagcaatt ttgccagaca cataacagta
    37801 cttcctgcat caagataaac ggatctgcct ttttcaatca gatgaatggt tttttgagct
    37861 atgactgctt ttttgtcaac attttctgac tgccttatca tatattcaat ttcatcttct
    37921 ccagcttttt taaaccgatt tatgcttacc gcgcctccat gcgttctgac tgcatacccc
    37981 atttcttcaa gaagaataag gtcccgacga atagtcattt ccgaaacttc aggaaaaacc
    38041 gttttaagct gtttgagaaa aacctcttga gctacatcta acatctgctt aatttgcaat
    38101 tgcctctgat tcaaaacgct acactcctaa tttatttatt cttattgtct cgccttctgt
    38161 ataatttaca gatgcggtat ggcccttcag tggaactatc ttctgatgga tagcatcaat
    38221 tatccaactt ggttgcggga agaagaattt ttcgtagtct gctgcaggtg ttatccagtt
    38281 ttttgaacct actacaacag ctggagcatc cagataatca aagcaaagtt cacttattgt
    38341 tgccgccata tccttaagat gacttcctct ttcacatgca tctgatgcaa gaactattct
    38401 tcctgtcttc ttaacagact ctgcaactaa ttcatagttg aaaggtgaaa ctgttctggc
    38461 atctattact tcagcttcca ttccatattt ttctttcagt tcatcagcag cttctagcgc
    38521 tgtgtataaa gttgcaccaa ctgtaagtat tgttacatct tttccaactc ttttaatatc
    38581 tggatcacca ataggtatct cataatattc tgttggaact ccgccttcat ggaacatctc
    38641 acccttatca taaagtttct gactttcaaa gaatattact ggatcggtac cattcaacgc
    38701 tgcattgagc aatccctttg tgtcatatgg cgttactggg aatacaactt taagtcccgg
    38761 gatatgtgcg cataaactgg tccagtcctg agagtgctga gcgccatact ttgcacctac
    38821 gcttactctt actataacag gcatgtttaa tatgcctgca ctcattgact gccatttagg
    38881 taactgattg aacacttcat ctcctgctct tcctaagaaa tcacagtaca taagctccac
    38941 taccgctctt cctcctgcca tagcatatcc aactgctgaa ccgactatag ctgcttcgct
    39001 tatggatgag ttgaaaagtc tgtgatatgg taaggaatca gataatcctc tgtatgctgc
    39061 aaatgctccg ccccattccc tgttttcttc tccatatgct attaaagtag gatcagtata
    39121 gaatttttct atcatagctt caaatatagc atctcttatc tgatatacct tgttcttgga
    39181 aacgggcttt ccatccacta tacccattct ttcttttttc tgaacctgct gataacgtgc
    39241 attctcttcc aaaggtgcat atgcatcaca aggcctgtct tccatttttt ctattcttct
    39301 atttgagaac atcaattctt caatagctct tggattagcg actaaatcca tctttggagt
    39361 aatcgtctca tcaatagcca tcttaaatat cctgaatata agtgaagctg tatcagaaag
    39421 tatagaatcg aattcttcat ctttggctat tccgcctttc gtaagcttct gtctgtattc
    39481 aatcaatgaa tcctgctcca tccacatatc tatctcttct ttggttctgt atgccgaagc
    39541 atcagatggt gaatggcctg caaatctgta agtcagaaca tctaaaagaa ctggtccctg
    39601 tttcttttct ataatttctc tctttctttt atatgcatca attacagcta aaggattata
    39661 tccgtcaatt ctttcagcat gcatctggtc aggattaacg cctgcaccga aacgtgcaag
    39721 aaggtcaaat cccattgttt ctccttttgt ctgaccaccc ataccgtaac agttattaaa
    39781 gaaattaaat ataataggaa gaccgccttt atgctctttc tcccataaag ttctgtactg
    39841 gtccatagaa gcaaaacata ttccttccca aactggtccg cagcccatag atgcatcacc
    39901 aagattagct attaccatgc cttttttttg gtttgctttt ttgaataatg cagcacctgc
    39961 tgctatatct gcacttcctc cgactattgc attatttgga aagattccaa atggtataaa
    40021 gaaagcatgc atagatccgc ctaatcctct atgaaaacca gtttctcttg agaaaatctc
    40081 agcaagtgtt ccataaagca ggaagtccat tgcaagctcc ttaactgtct tttggtcttt
    40141 ttgaactact ttcaatattc tgccatcaag ataattttcc attatatcca taagctcttt
    40201 atccgaaagt ttctcaatag cggacaatcc ctttgcaaga atctctccat gacttctatg
    40261 tgatccgaaa atgaaatcat ctattccaag cgtgtacgct tcaccaacaa cggaagcttc
    40321 ctgtcccatt gaaaggtgag caggacctgg gtatgtatgc tcaacagagt tgtattgccc
    40381 tgtggtctta acagcattaa gcatgctctc gaattcacga attatagcca tatccctgta
    40441 tattcgcatg aaatcatttt ttgtgaatat cttcttctct tcgtcaagcg tcttattata
    40501 tgcacagacg tctatgtctt taaatttaat cttacttttc tttcttaatt cagatggatt
    40561 aagtactaat tcttttgtca ttttatttct cctcgtataa ttttacttac tgttattgtt
    40621 tattacttcc attaaaaagc gaaaagcgct tctcttataa tctcacaaac tgtcggatgc
    40681 ggaaaaacca gttcctttat gtcttccact gtcatctgag tctctatcat catagcagca
    40741 ccatatatta actcagatgc atagttgcct atcagactta cgcctatcag cctgttagta
    40801 tctttttctc ttaatacttt acatattcca tttccgcctt cgttctcagc aacatatctt
    40861 cctgagaatt tcatggataa tttaaccgat tcaaaattca tgttcttctg ttttgctgta
    40921 gcttccgttt cgcctactga tgcaacttca ggatttgtgt atattactga aggtatagcg
    40981 ttatatctca taatatcttt cttgcctgtc atattattca cgcatacttc tgcttctctg
    41041 tatgctgtat gagcaagcat cgatattccg tttatatctc ctgcagcata aacttccgct
    41101 acattcgtac ggccaaacga atcggtaatt atagctcctc ttttgtctgt ctctacgcct
    41161 atgttttcca gccccagatc ttttgtagcg ggacgtcttc ctatacttac caatgtctta
    41221 tcagctaata tctcgtaact tttcccatct ttttcaaaaa taaccgagtt ctcagtaaat
    41281 gatgttactt tactgcctaa attaaagatt actccttttt tctcatattc ttttctcaat
    41341 aattctgata tctcatcatc tataggtcca cctatcttgt cgagcatctc aactactgta
    41401 acggtacttc cgacagagtt atagtatgaa gccatttcca accctataac tccgcctccg
    41461 actataacta aattttcagg tatttctgtt aagtccagta cttctctgtt tgttagaacg
    41521 aatccttttt catatccttc atgtaatcct ggaataggag gcattatagg tactgatcct
    41581 gcacatatca gcagtctgtt tgctaaatat ttctcgttat cagcgcttac tacataaccc
    41641 tctgatgatt ttttctcaat tttggctgtt ttttcaacaa cagtaacatt gtgatgcttt
    41701 aaagctgctt taacgcctgt taccaaagtt tttacaactt tgttttttct ttttataaca
    41761 acctgctggt caattgaaga ctctgtaacc ttaataccat attgttctga atgtttagtg
    41821 taatcatata tcttagcaga atacagcaaa gcttttgatg gcacgcatcc ttcattaaga
    41881 catactcctc ctaaatttct cttttcaaac aacagcacat tcaaaccttt tgctgcagct
    41941 ctttcagatg ctagatatcc tgcaggccct ccgcctatta ctattaaatc gtatttcatc
    42001 aaaaacctct ttctatttcg caagcatcat ttcaaaattc tcaagatttg agcaaagttc
    42061 ctgtaaaaat ctggcagcag gagctccatc aaccactctg tgatcaattg tcagagacaa
    42121 tcccattgcc tgataagctt tgagctgacc gttaacttcc tttacccttg tagtaagacc
    42181 gcaaacgcct aatattgctg tctgtggagg attaataacc ggagtgaatg attctacccc
    42241 aagattgcca agattggtta ttgtaaaggt tcccccactg agaagatcag gacttatact
    42301 tcctgatttg ctctttgcag caagttcctt gacttgtagt gaaatatcat ttaacgattt
    42361 catatcagca ttgaaaactg taggaaccat taatcctctt ggagtatcta tagctacacc
    42421 catatgaaca gtattaaacc gtttcataac atcattagca aaatgcgcat ttaagtcttt
    42481 atgagaaaga agagttttag ctgcagcata aaccactata tcattaattg ttatgttagc
    42541 aagtcccagt ctttctgcat ttgcttttac catagctctg taatttaata tgcctgtagc
    42601 gtcaaatgtg gaattcattg tgagctgagc catttcagaa atggatgaat gcatagtacg
    42661 tgcaataacc tttcttatat tcgttagctt ttcctctgta tattcaacaa atccttcttc
    42721 cgttttcgca agcatgtgtt cgggctgaac ctgtttactt aaatcatcaa ctgtaaccct
    42781 tccaccaata ccggtagcat tatatgatgc gccttcaaca tatcctgatt ttgcagcgga
    42841 tgtaacttta gtgccatctt tagctagttt ttgaacatct ctttctatta ccctgttgtt
    42901 aggaccagtg ggttctgcat acaaaacatc aacacctgtc ttatctgcta aagactttgc
    42961 acgaggtgaa attttcaaac tttctgaatc atttgcttta ataggttctt tttttctctc
    43021 aggttttgaa tcttcaactg ttttagtatc tttattttcc tgtgattcag gcttgctttc
    43081 ctgtgctcca tctagagtaa atccagagat atcctcacct tcttcaccta taacgcacat
    43141 atttaaaaga catggtacat catcgccttc ttcaaaatac gtttctaaaa ggacaccttc
    43201 aacttttgat tcttcatcga aagttgcctt atcagtttca tatgtaaaca atatgtcgcc
    43261 tacattaact ttatcgcctt ttttcttgtg ccatttggaa actacacatg attctactgt
    43321 atttccctgc cttggcataa taactgctgt tgccatattc tatcctccat tcttaatgca
    43381 tagcaaagaa ctgctcatgc tgttagattc taacatgttt atgttattta tgcaataaaa
    43441 atttacatta ttttttgtta tatatccatg tcttttaaca tatccaaaac tatttcatta
    43501 accttggaag gattcgcaca accttttgta attttcatta cataacctac aattggcccc
    43561 attgcctgaa tgttcccgct tttaaatgac tcaacagact tagtatttaa agaaatggct
    43621 tttgttacat attccttaag cttctgaata tcgttaatct gaaacatatt ctttattttt
    43681 acctgttgct ttggatcaaa gtcttctttt aaaagcatat caattagttt ctttgcagtt
    43741 gttgtattta tctggtcttt gtctgtcata tcagctatca cagcccatct ttcagctttt
    43801 atctctagtg ccttttctga cagcggtatc atgaggttag ctagaatttt gtatgctgat
    43861 gtcaaactgg ctgctgtttc gtaaagtaca gaaattttca tatcagatac aagcatttca
    43921 ctgtcaaatg aagataaacc gtactttttc ataatctgtg gtttcttgtc ttttgccagt
    43981 ataggcagta ttctttttat ggactgtaca tactcatctg tcagattaat cggtaccagg
    44041 tcaggatcag gaaaatatct gtaatcatca gcatcttctt tttctctcat tgaatatgtt
    44101 ttcttatttt tttcatcata tcttcttgtc tcctgaacta ttttcttatc agcaagcaag
    44161 ctgtcaacct ggcgtacaaa ttcgtattct atggcttttt gaatgaatgc aaaagaatta
    44221 agatttttca tttcagttct tgttccaagc aatttgtcag tttcttttct tactgaaaga
    44281 tttacatcag ccctgaatga accttcattc attctgcagt cagatattcc tagattcaat
    44341 aaaattgttc tcaatttatt taaaaaaatg ccaacctgtt cagttgattc gaaatcaggc
    44401 tctgtaacta tttctataag gggaactcca cacctgttac agtcaatcat ggtacccttt
    44461 tcatcatcat gtataagttt gccagcatct tcctctaaat gaattcgtgt aatatttatc
    44521 cttttttcac cttcagctgt tactatatca agatatccgt taatacataa cggcatgtca
    44581 tattgggata tctgatatgc ttttggaaga tccggataaa agtaattctt tctgtccatt
    44641 ttactgttta aactaatttt gcagtcggta gccagaccgg ccattattgc atactcaaca
    44701 acctttatgt tcattaccgg aagagtacca ggcattccaa gacatatcgg acaggtctga
    44761 gtatttgcac ttgctccaaa atctgtagaa cagctgcaga atatcttgct atcagttttt
    44821 agctccacat gaacttccag tcctattgtc ataatatatc ctttgtaact agtcatttct
    44881 gttctccttg ttattctgtt cgtatgcata tcctaactga taaagcatac tttcggaaaa
    44941 tctcggccct ataagctgca taccgacagg taatttttca tctgtcattc cgcatggaac
    45001 tgacaaggca ggaagacctg caatatttac aggaacagta aatatatctt ccatatacat
    45061 ttttacagca tctttttcga tattatccaa agaaaatgca gtagaaggag taacaggtgc
    45121 taagataaag tcacactttt taaaagcttc atcaaactgc atgctgacaa gcgatcgtac
    45181 tttctgcgct ttgttatagt actggtcgta gtagccctga cttaaaacaa atgtcccaag
    45241 cataatcctt ctttttgcct cagaaccaaa tcctaagctt ctgctttgtt cgtacaaact
    45301 ggatatatct ttgtattcat cagctctgaa cccgtatctt attccgtcat atcgcgcaag
    45361 attggaagaa gcttctgcac ttgaaattat ataatatgca tatatggccc agtctaaaga
    45421 tggcatatcc agctccacta tttcgatgtt ctgtgatgca taccatttta ttgctttatc
    45481 aatccttatt gaaacagaat catctatacc ctggctacag aactgtttta taattcccat
    45541 tctttttttt gatatatctt tttcaataca gcatgttgat gtgtcatata gtctttttac
    45601 acttgtggaa tctaatacat catgaccttt tattgcatca aatactaaag cattatcata
    45661 aacattttta gttatgggac ctatctggtc aagtgaagat gcaaaagcta caagaccata
    45721 tcttgacaca gagccatatg tcggcttaag tccaacaaca ttgcaaaatg atgcaggctg
    45781 tcttattgaa cctcctgtat cggaacctag tgaaaacaca gtttgcgatg ctgcaactgc
    45841 tccagcagaa cctcctgaac ttcctccagg ggatttggat aaatcataag ggttgtgcgt
    45901 tttatgaaaa tgggatgtcg tcgtagttga acccattgcg aattcatcca tattcaattt
    45961 acctagaaga accataccgt cttttttaag cttgtcatat acggttgcat catatggggg
    46021 tataaaatcc tcaagcattt ttgatgcaca agtagtctta atgttttttg tacatatatt
    46081 atctttcaaa gctcctggta tgccataaag taaagataca ttatcagtac tagttaactg
    46141 ttcatctttt ttctttgcat cggctaaagc cagatcttct gtaatagtta tgtatgcctg
    46201 tatatcagtt tctttatccg aaatattatc aagatatgct tttgttattt ccacacagct
    46261 cagttctttt tttattataa gttcatgaag gctcaatata tcttttttta aaacatcttt
    46321 catatcacac ctccgccttt aaaactgcag gtactgtaat gtatccgtcg ctatgtgttt
    46381 ttgcatttga aagcaattca tcctctgata gtgattttaa agctttatct tcacgaaaaa
    46441 cattttccat ttcaaaacaa gtatatttct gctcaacatt gcttgtgtca atgtccatta
    46501 tattggatgc gaattttata atattctgca tgtcttcttt taagcttttc ttttcctctt
    46561 ctctcaaaga cagtctggca agctttgaca gtttatcaat gggtatctct atatcattct
    46621 ttcttttttc cttcatctca aaatgatcct tgtatgatat atgaagtgct ttcaactgtt
    46681 cattttccac atagtcagga gctttggtca taacgcatga accgtctttg attttaggaa
    46741 atgctatgac atcccttaat gatgaagctt gccgtaatat cataataagc ctgtccagtc
    46801 caaatgcgaa tccgccatgc gggggtgttc caaagctaaa tgctttaact aaaaacccga
    46861 attgagactt tatctgttct ttattcattc ctaacgcttc aaacatcagg ttctgcatat
    46921 tgctatcatg aatccttata gacccgcttc caagctccgt cccatttaat actatatcat
    46981 atgacaatgc cctaatgcgt tttttatctg taagcatata tttcaaatca tcaggatgag
    47041 gacaagtaaa cggatgatga gcagcaacaa atcttttctg ttcttcagaa tattcaaaaa
    47101 gaggaaagtc tgttactata acaaaactat atgcttgcct atcacacatg ttgtcatttt
    47161 gagctatatg tattcttaaa ctgcccagta tttttcgcga attctcaaaa gtgtcagcac
    47221 agaacacaat aaagtcattt ggcttagcat ccatttgagt gaataattca tccatttcct
    47281 ttttctctat atattttgta aggatggaat ttatcgttcc atcagattca atggcaatcc
    47341 acgccattcc ttttgctcca aattcaagag cttttttggt cagctgctca atttcacctc
    47401 tggtcattga tgctttgttc ttgtagttta tagccctgat gcattcagat tttttgaaaa
    47461 tgctaaaacc tgatttctta acaatttctg ttacatcatg aatttttaaa ccatatctca
    47521 aatccggttt atcgcatcca tatgtatcca ttgcttcagt atatgtcata cgtaaaaatg
    47581 gatctgatat ctctttacca atagattttt tgaaaatatg cttaaataac ttctctaaat
    47641 gtatcagaac atcctcctgt tcaacaaagc tcatttccat atctacctgt gtaaattccg
    47701 gttgtctgtc tgctcttaaa tcctcgtccc tgaagcatct tgctatctgg taatacttat
    47761 ccattccgcc aaccattaaa agctgcttaa atatctgagg tgactgtgga agcgcataaa
    47821 acttctgctg gtgcactctg cttggcacaa gataatctct tgctccttca ggagttgatt
    47881 tagtcagtat aggggtctct atctcaacaa aaccatcatc atcaagatat tcccttataa
    47941 ctttcgatac catatgtctg aaacgaaggt tatcagatat ctctgttctt cttatatcaa
    48001 ggtatctgta ttttaatcgt aaatcttctt taataaatgt attatcatct attgcaaaag
    48061 gaagatttga tgcttctgac aaaaccacaa tatccgatac tttcagttct atcgttcctg
    48121 ttttaagttt gtcgttgtat gtctcctctc ctctgatcct tattatccca tgcatctgaa
    48181 ctactgactg caattttatc tgttcagcaa gcgaaaaaac atcatctgta acatcattta
    48241 aatcaaatac tgtctgaaga ataccttctc tgtcttttaa atcaatgaat attacaccgc
    48301 ccatatctct tttggaccat acccatccat tgacagtaat acttttgccg acataatctt
    48361 cacttattaa cccgcaatat tgtgttcttt tcatgacacc ttctcttcct ggcaacaaaa
    48421 aaacctttcg tagccaaata aaatatttat ctgggacgaa aggtaattat ccattccgcg
    48481 gtgccacccg ttttgatcat aatgatccgc tcttttcagc actgacatgc ctaacggtta
    48541 taacgttccg taatccgtct gagcctacta gtatatactt tcggtcagct gctcgaagat
    48601 gttcttcatc ttaggtttct atacccgcat ctcaccattc gcggactctc tttgatatat
    48661 accctgacta ctcttcttgt catcgcatta tttttcatta tgctaacact aaataaaact
    48721 agtgtcaata tattttttca atttaccctg cactacttta attctgtgtc tagaaattac
    48781 acttcgtttc taatatgtct gtaattacac atattactta ttaagttttt ctacatcctt
    48841 ccaggcgccc gatataataa ggtgagtatt ctctttgaat atgtagtttg gatttgataa
    48901 aggatatatc tttccgtttt ccttatatgc taaaacgtta acattgtaat tggatcttac
    48961 attgacctgt gctatagtct ttccaaccca gttttcagga gtttctatct cacatatgga
    49021 ataatcctgg gacaattgaa tatagtcaaa catatggttt gcactgaatc tgtatgctgc
    49081 tctttgtgcc atgtcttttt ccggaaagat tacttcatca gcaccatttc ttaaaagaaa
    49141 ttttgcatgt atgtctctgt ctgcttttga tactacatat ttggccccgt tatcctttaa
    49201 caaagaagtt atctctaaag atgactgaaa attatctccg atacaaacaa agcatatatc
    49261 aaaattgtta atacccaaag aacgtaatac attctcatcc atacaatctg caacctgagc
    49321 tgatgtcaca tattctgata tatcacgtac ttcattctct tctttatcaa caaccattac
    49381 ctcattgcct aattcagcca ttttcaatgc aagattctta ccaaattttc ctgtacctat
    49441 tattaaaaca gatttcataa atgttctcct aaccaattac aattttttct tcaatgctgc
    49501 gaatattggg ttttattcta tccccagcaa atgcgcttaa taccgtgagg cttccaactc
    49561 ttccacagta cattaacaat attatcacag tttttgatat tgtgcttaag tctggcgtaa
    49621 ttcctctgga taaaccgact gtacctattg cagaaagtgc ttcaaacact gtttccttca
    49681 tacctagctg ctgtgttgac attatcagaa atgcaccaaa tagtgatagc agcaaataca
    49741 aagtagttat actgaaagct tttctaggaa catattcttc cagccttctt cctcctgcat
    49801 ttaaatcctg agaacgtgtt acatatgctt taactgtcaa tattattaca aaaagtgtgg
    49861 taactttgat accacctccg gttgaccctg gatttgcacc gattatcatt aagagcattg
    49921 tcataaatgt tgttccgtct ccaagcgaag ctgtatcaac agtattaaat ccggctgttc
    49981 ttggagttat tacctgaaat actgaagcca taatcttctg tcctgctgtc ataccttcta
    50041 atgtatttga attttcaagg aaaaagaata taactgctgg aattaatatt aatacagctg
    50101 ttccaataag aacaatttta gtatgaagac gatatttgga aaatttaaat ttattgttca
    50161 ttaaatcgtc ccagactatg aagcctaacc caccaattac aatgaggctc attaccacaa
    50221 tattgactaa aacatcagta ttaaatgtta ttagagaact tctggcttca aataccccca
    50281 tcaaatcaaa tcctgcatta caaaaagccg atatagaatg gaatagtccg taatatatac
    50341 cctttgcagt tccaagaaga ggaatgaatc taatagacag tattgctgca gatacaattt
    50401 ctattgagaa tgtaataaca ataatctttc taagaagctt aactacaccg cctatctgat
    50461 tagtgttcat tgtctccatt aataatccgc gttctttcaa tccaattttt ttcccaataa
    50521 gaagagatat catagtggcc atactcataa atcccagtcc gcctatctgt ataagcgtta
    50581 gtattacaag ttgtccgaac aaagtgaact gtgtgtaagt atcatataca acaagtcccg
    50641 taacgcataa tgatgatgtc gcagtaaata aggcatctat aaaagggata cggataccgt
    50701 ttcttgaagc aacaggtaat gaaagcagga aagttccgat tagtattatt actacgaaac
    50761 caaccgcaat tatctgcatc gttgatattt tttttgtttt ctttagttcc atttgcttaa
    50821 agcaactcct taaccattct tcctatggcc tgtgccatcc ttacaaaccc aaagtcattt
    50881 ggatgaactc cgtccacagt acaacaatcc ctatagtcat cctgaaaaag gcttctccca
    50941 tcaatataat aaacattcct atcgcctttt tgcatagcat tcatataggt cttataaatt
    51001 atattccttc tttgtatatt gtcaaatgaa taatcagaga aattcggttt tgtcacaaat
    51061 accactggaa ggccaggttg cttgttacgt atttttttga agaattcttc atgagttttt
    51121 tctaaatgtt ccggtgtagg cgcattatag tcatagtcca ttacaaacat actcatatcc
    51181 attttagcta tgtattcagc catgtctgat tcacctaagg ctgaaccgga aaatcctaaa
    51241 cacatatagt cagtatcgca catcctggat attattgctt catatgaatt gccagtacgt
    51301 gatgcacacc ctccctgtgt tatagatgaa ccataatata caacaggctt ctcatgtgta
    51361 tatcttatgc cttcgcctat taatgattct ttacccaccc cgatatatac gctgtccacc
    51421 tgattgtata aaggaaaata tatagtaaga ttctttaaac cttcatttac aaatcttatc
    51481 ctcatctcat accagtcttt tgtatctaca catggaacaa acttgtcaat aaaaacgctc
    51541 ttcccattaa tttcctgata tatatcaaat ccactggaac caagaagggg catatgattc
    51601 attctgttta tctctttggt aaaaacctga atacataaat atggcgaatc tgtactaaaa
    51661 cgtattcttc cacctgatgt gtgaagatag gttttttgaa caccgctgtt acatttagca
    51721 gctacttcgt caggcagtct tttaaataca ggctgattta gtgggtcata taatccgtat
    51781 atttcaaacg gttttttacg tatatccaga taaataaggt cctcatcatc aagattagtg
    51841 tgaggcttca aaccttcata atatgatatt gcttttttca tatagtccct ttcttattat
    51901 cagctctcca gctgatactt tgaaatttca acgctgtcag taccatagtc attattaaaa
    51961 cagcctgtac agaaaggaag cgtacatttc tcacatgctt ttttaagccc ctcaacactt
    52021 ataaatccta aactgtcagc tcctattctt tcacatatct gctgattagt tatctgatta
    52081 gctattaagt ttttttcatt gtcaatatcc gtaccaaaat ggcaggtatg aataaatggc
    52141 ggtgaggata tcctcatatg aacttccttt acgccagtac ttcttaatga agaaataatt
    52201 ttagcgcttg tggttcctct gactatggaa tcatcaacaa gaataatact ctttccttgc
    52261 acatttgctt ttaatggatt gagttttatt ttaaccgcat gttccctttg aagttgtgaa
    52321 ggatatataa atgatcttcc aatatatctg ttcttaacaa atccagatac taaaggcaaa
    52381 ccactttcat atgcataacc catagctgct tcaagaccgc tgtcaggaac tccgcatact
    52441 acatcagcat ctataggatg ttcttttgca agcatacgcc ccatattaaa acgggcttca
    52501 tatacgctca gaccatctat aatggaatca ggacgcgcaa aatatatata ctcaaatatg
    52561 caaagaccct gtttattagc agtaagttca acattactgt actgaatttt accattttca
    52621 ataataatta cttctccagg cataacatct cttataaact caaaaccgca gctgtccagc
    52681 gcacaagatt ctgaggctgc agctattccg aattcgtttt taccgataca taaaggcctg
    52741 aacccgtggg ggtctcttac taccacaagt cttttatctc ccgttagtaa cactaaagaa
    52801 aaagcgcctt taagaagttt tgctgcttca ataacacctt ttagcaaatc cctgttgctt
    52861 attatgttat atgcaatcaa agaggatatg acttctgaat cagaagaagc tgaaaagtcc
    52921 agtccctgtt tttccagcat aactttaatt tgttttgcat tggttatatt tccgttatga
    52981 gcagtagcta ttcttccagt cagatattct gtcaaaaaag gctgaacgtt tttatttgtg
    53041 ttcgaaccgg tagtcgaata tctggcatgt ccgataacgg ttctgctttt aggcagttta
    53101 tctaatacct tttcagttaa aacttcatta accagaccag tattcttttg aattataatt
    53161 gactggttat gtgacagcgc tattccagcg ccttcctgtc ctctgtgctg caaagcaagc
    53221 aaaccattat acacagcacc tactgcttct tcgcacttaa gacttactcc cattatcgca
    53281 cattcttctt ttatcttatc caaaaatcca cccattctcc catactaaca atatattgtt
    53341 aattatactc tattttcccg cttattgttg ctataactac agttcctaaa tcaatttccc
    53401 ttcctttttt cgctgtagct gtttttgtga ttacctgagt atctatattg taattatctg
    53461 ataagccttt tgatttagca tctttcaggg caacttcctt tgctttttct tcagcatata
    53521 aaagtgcttt gctaatatca ctttccttta cctttccctc aggtccgaaa atgagatagc
    53581 ttccgtcata ttctgctctg atttcaattt gatgagccga acggatgtta cttactactg
    53641 cacctattgc attggcaact tttgcatttt caggtatcac acatcttgtt tttaacgctt
    53701 ttgctacatc aggtaaaaat atgtgagttg gagcaccaat tcctaccaaa gaaaaatctg
    53761 tcttaaataa agcggggata ttgttttgct ctttgtcctt aaatgaattc tgaattaaac
    53821 ttgttatctg ttcatccatc ttgtttttga atatatcagg atatttgtca catagaagta
    53881 ttcttactat attctcataa agtttaagtt ttacagactc ataaaccata tcacagaatt
    53941 gctcttgttc tatttccaaa ccagtttctt caagtatgca gtttagtata tattcaaggg
    54001 caagtttggg agcagttgta tcaaattgag taaaatcacc tttcaagtgc attatatctg
    54061 tcggtgttaa tcctattctt ataataatcc cttctgtttc cagcctcttt gtatttatag
    54121 tatatacatc acgacctaca gctaaagcgg ctttttcaat taatataggt tcttttttaa
    54181 gaatattgca caattcaatc tcatcatcag aatatatcaa tgtatttttt atatctttct
    54241 gaagaacaag gaattcataa agcggatgac tgtgataacg tccattgtct attaattcat
    54301 ttaactgttc ttttatacaa ggccatttct ttgaagccac gcaaaaaggg attctccttt
    54361 gtgataatag aacaggtctg ccatgctcaa ccctgatacc gctgtctccg cctaagccga
    54421 aagtatctat aaaaacacca tgaacaaatg tcttccatga gcctatcctg acaccgctgt
    54481 cagcgttaag cggaacaccg tttttgacga tggatatatc tgttgttgta ccacccatat
    54541 ctacaattag gcagtcagtt tcttttgtgc agttaatccc tcctgtaaca gaagatgcag
    54601 gacctgataa aattgtttct acaggtttcg tcatggaata ttgctctgac atttttgtgc
    54661 tatcacttct tattattgct attggcgcaa aaattccttt ttcctctaac gcctttttta
    54721 tgctttcaac aaactcctga attataggaa taagttttgc gtttagaaga gtactggaac
    54781 ctctctgtat ggaatttaat tcgtaaaaaa gttcgtgtcc gcatactact ggtatatcat
    54841 atctttttga aacctgctct tttgctttct tttccagcat tgctccatta ttcatcgcaa
    54901 atacttctac aatacctaat ccctgagcat ctttcagcca gtctgccaga ttatcaaaaa
    54961 gcttatccca gtctggttct tcggctttag agccattaaa atcacttgca ttatcacaaa
    55021 gatatacttc atctttatta accagcccat agtttttccc taccatgtca aaaacttttt
    55081 tgtctactcc gaaaaacatc agtttagctc tgccgcctct gttttcaaca catgcatttg
    55141 ttgccaaagt agtggacaga gccactattt caatcttatc aaaatatgac tggtcaagtt
    55201 gatttaatgc gtttattatt ccttcttcaa gaaaatcatg ggttgttcga gcttttgaag
    55261 aacaaacaac gctttcgctc gaaaaatcat atattactgc gtctgtgtat gtaccgccag
    55321 tatcaatacc gattcctagt gccataaaac acctccaaac ggtaacattc tacataactt
    55381 tgaatgattt taccatacat atttgccaat tttacagata tataagtttt ccgccttctt
    55441 ttctggattg ttctgccagc attacattcc tgtggctcac aattgtagta tctataaatg
    55501 tcagatcatc ttttttgatt ggttcattat taagcaattt aatgaagttt ttaacaatgg
    55561 caatgtctcc ggatatgtgt acaaagtgta ttacacctgc tcttactact ttcttacttt
    55621 ttccgaattc attgataata aaacggccct ttgactcatc acataaaata tccgcttttt
    55681 ccatatgaat actggtccgt ctgtaattaa gccttgaaaa agcagtaaca ttgagattga
    55741 catatctgtc atttttataa tccattgtta ctacctgatg atcacatgca ttgttatcgc
    55801 atctgtatac acatcttcca tataaaccgg tccttaatga ctcatattta tcttttctgt
    55861 tatattttgg cttaccagtt attacatttc caagaaactt aagaaaagta aacctccata
    55921 taggtgcagt tatatatagt ttttcagcat catatggaca tttgctttta acatgccttg
    55981 gacattctaa acatattttt gcactatcct tcggtgcatt atcttttttg aaatatgaaa
    56041 gacttccata tgatgaaata gactggcatc cgtcacccat cagccagtgt ataagatcaa
    56101 aatcatgaca acattttgcc ataagcatag gtgtagtagt ttctgagtct ctccagtttc
    56161 ctcttacgta actgtgcgca aaatgaaaat atccgatgtt ctcagtgtgt tcaattgaaa
    56221 taattttacc gtatttttca cttttcacta tttgattcat cttcttaaag aatggggaat
    56281 accgcataac atggcataca actacatgaa gtcccttttc tttggccttg tctctaatca
    56341 tctcacattc aggtatattt gttgatacag gtttttcaag cattatatga tatccgcatt
    56401 taattgcagc catagcatgt cgaaaatggt ctttgtccat tgtacagata tatatcgcat
    56461 ctgcaagttt accggctttg aaaaatttat gttcatcaag gtatcttttg tctttatcta
    56521 tcttgtattt atccccatat gagttcagcc ggtattcatc agaatcacat aatgcggtta
    56581 ttctggcttt atttccatta aatagccttg tccatctcat atatccgctt cctctgttgc
    56641 ccaaaccgat aacagcaata gtgatcattt ttcagcctcc cattttttcc atgtttcaaa
    56701 ctcataccca attgtagcct gattcccgtt tcttacaaca ggtgttttta ttatctgctg
    56761 gtttaaaagc aaaatatcaa atttattatc gttctttgca tatttaaata aaagtagctt
    56821 atccttatct tttgattttt catcaacgag gttatctatt ccaccagtgg ctcttataac
    56881 tttctccagc tcgcctttgc tcaatccctt ttcagccata tctacgtatt gaaaagatat
    56941 ttttctttct ttaaaaaatc tctcggcttt tttagaatca aaacacttac tttttccaaa
    57001 tatctgaata ttcatatctt cattccctat gtaattattt caataactta ttcattgata
    57061 cagtatattt taccataaaa tcacatcatt tcttgtatcc ttttgtcatt aaagctataa
    57121 ttgtttcgat agatactata atattgaagg aaataaatga aagcatttct gataaaaaac
    57181 agaagattga tgtacctttt cataccagta cttcttcagt tggcttattt tattatttta
    57241 aggacattga cgcctacaaa aagttatcaa tggtttttaa cggatataga caatattatt
    57301 ccatttaaac catttatgat ttatggaacg atattatgga tattaatttt cttatccgtt
    57361 ttcttatacc tctacttctc agcaggaaac agctattacc gttttaccgt gtgtatatca
    57421 tcaattatgc ttatatcagt tatcttatat ttatcttacc ctgcgcagat gatttatcct
    57481 gaaactaccg gttttttcct tgctgatatc ctttactcag atatcttttc aatacctttt
    57541 ttaaatatgc cgacagctat tatgacctca agtatattct tcttgctcaa ccataaaaga
    57601 aagcataagc attcaaaata tgtattattg ctaatattat caataatact actgttgatg
    57661 tgggcagttt ttgctctgta ctcaaaatct gcagtttatt taggtatctt atccggaata
    57721 ggaacaagca tgattgtatc ttttgcaata tgcagtattc ccttcaaaca cactttttaa
    57781 agttcttttt taataaattt tccatcatac tcaaatatat gagtataccc aatatccaaa
    57841 agagtatctt tagctttatc ataaccatat tgaagaccag atataaagtg acagtctgaa
    57901 ttaacagata caggtatttt ctttttaaag cactgcttca atatatattt actcggatac
    57961 attacatcaa tattctttgc cattgcgcct gtattaactt caacacatga tttatttata
    58021 cagtttaaag tgttctcaat ctgatttttg tattcctctg tatctggatt aaacaattcg
    58081 taattcctat ttctcttttc taggacatca atatgagcaa tcattatcgg atcatactct
    58141 tttatcattt cctgcacagt ctgataatac tttttataaa aaagcactcc attattatta
    58201 aaaccatttg atataagctt ctctacttcc tcctgcgaat tatcagctgt accatatgaa
    58261 tctgttattt cagaatatat ataatggacg cttccgataa aatagtccag attcagatta
    58321 tcaaaatcta tatacttttt aatattatac ccctgaatat agtcaagctc catacctgca
    58381 tatactgata tatgtttttt atatgttctt tttaaatagt ccagctctgc tatatactta
    58441 gaaagatcct ctttcttcat cgcccatgtg ctgttaaaag gtaccggtga atggtcagaa
    58501 atcccaattg acttcaatcg taatctgatt gcttcttcaa tatattcata aactttattt
    58561 acaccatctg aataattcgt atgaagatga atggtactta atttattcat ccatgttcac
    58621 tttctctttt ataatataca aaggcctgtt tttgctttcg tcaaatatcc tggctatata
    58681 ttctccggta atacctaaca ttataagctg tatacctcca atgagcaaaa ttgtaaccat
    58741 agtagaagcc cagcctgtct gcatctgtat accgacaagt ttctgaatta taactataat
    58801 agcatatata aatcctactg ctgccataaa tatacctatc catttagtca gtttcagagg
    58861 cttagtagaa aatgatgtta ttccgtcaac agaaagttta agcatttttt taagaggata
    58921 ctttgtttta cctgcatatc tttcttctct ttcataataa attccagttt gcttaaatcc
    58981 tacccatgat accagtcctc ttacatatct gtttctttca gtaagtcctt ttaatacatc
    59041 acagacctga cgactgataa gcctgaagtc accagtatca acaggaatat ctacttcaca
    59101 catatttttt aagaatcgat agaacatttt tgcagaaatc tttttaaaga aggattcacc
    59161 ttttcttttt gatcgaattg catatactac atcataacct tcttcatatt tttctattag
    59221 ctttagtaca acttccggcg gatcctgtaa atctgcatcc attacaatta ctgcatcgcc
    59281 ttcagcatta tccatgcctg cagttattgc tgtctgatgc ccgaaatttc ttgaaaatga
    59341 tataagctta agatgcttat ctttattaca atactgctta actatttctt cagtagaatc
    59401 gctgcttcca tcatttacaa atatgatctc ataatcaaat tcattattat gcataacatt
    59461 agtaactctt ttaatacatt cgtgtattac ctcttcttca ttgtatagcg gtattacaag
    59521 agaatacatt tttttcataa agcacctcat tcgtttaata tagatattat acattttttt
    59581 agattacttt tacatatgat gtatcgaatt agtgattatt ttcatgattt taaggaaaat
    59641 gttattgata tgtcatatta tatttatatt taatataatt tatactttaa tttattgaca
    59701 ctaatgttac aatatgctaa tatatggtac atattagaag taaaaaattt ttcaaggagg
    59761 tagaacgtgg attctttata cagaaagtat ggtcgtagag tacgtgaaaa aagagtcaaa
    59821 ataaacatgt cacaagaaac acttgcagaa aagtgccagg tatcaacatc ttatataggt
    59881 cttgtcgaaa gaggagaacg taaaccttcc ttattagtgc tcgttaggat cgcaaatgct
    59941 ttaaacgtga gtactgattc tcttttattt gattctctta gacatattgc atcatcaaat
    60001 aaggatcgca taatgatgat ggttaacgat tttgatgatc ctcagattga tacgacattt
    60061 gagattgtta accagattca ttcatatttg aaaaaagatg tgcctaaaat tgatagagtt
    60121 gatatcgatc agatattaaa tgattaactc tcctcagaat taattaaatt cttcaatttt
    60181 ctaaaaaaga cattcctgtc aagcattatt ttatggccac agccaaggca ttccaaacgg
    60241 aaatcagcgc ctacacgcaa aacacgccat ttaaccgatc cgcaggggtg ttttttttta
    60301 agttcaacaa tgtcatttat ttgaacatca ggtctcattc ttcaatctcg aataattctt
    60361 tttcagcacc gcattccgga caaatccaat ctattggtaa atcgttgaac tctgttcctt
    60421 cgggaataca gcctatttca tctccattat caggatcata tatatatccg caaacagtac
    60481 aaatgtatct atccatacag cacctcttgt tcttttctat gcaattatat catcataaat
    60541 aattaattta tataaatatg taactattcg attacaaaag tgtcaagata acctcttaag
    60601 aaatccaaat cctcatttga aaatgcaccg atcataatag tcagaaaata cccatttttt
    60661 ataactccgt acatatcaaa aaccatatac aaatcattca tatcaagagt aatacttcta
    60721 tatgcgaatt tccggttact taaccataca ttcttatgat ttgatactgt aacttctgct
    60781 ccttcaatag attgatatgc agatgacttt tcatatgcca tatactctat gtatgtgtcc
    60841 ttatcagaat aaccgtattt gcttgcatct tctacagata tcattatgtg agagttgttt
    60901 tcagcatcat ctatcggttt aagcgagaag aaagcaagat atgttatgta actaaccata
    60961 tcttcatctt ctaaaagatg agttatgtca aaacttgttt ccagttcctc ttctgtagct
    61021 atatgataac cttcaggaac ctcaattgag atacccatat ccttattggt ataaataccg
    61081 tcaacaactt cgcctcttcc gatttccaaa ggttttacaa tttctgtaga tttactgtca
    61141 caacctgcta acatactgat tgataaagct attattaaaa ttagtactat ggttcttctc
    61201 attactattc tcctattatt ttgacaagga cctttttttg tcgaaggcca tcaaactccc
    61261 catagaaaac ctgctcccat ggcccaaaat ccatcttgcc ttctgtaatg gcaataacaa
    61321 cttccctacc cattacagtc ctttttaaat gagcatctgc attatcctca aacccattat
    61381 gagcatattg ggaatatggt ttttcaggtg caagcttctc cagccatttc tccatatcac
    61441 tatgcagtcc tgattcatca tcatttatga atactgatgc tgtaatatgc atagcattac
    61501 atagcaatag gccttcttta ataccgctct cataaagaca ttgctgcaac tgtggggtta
    61561 tgtttacaaa ccctcttcta gttttaatat tgaaatacag ttcttttcta aaactcttca
    61621 taatcacttt atatatttta cagaaaggaa ggtgaaaata caactataac aatatgtcct
    61681 cctcattctt gcataatata ttctcctcca tgatagcata ttattctttt aacattcata
    61741 ttagttatct tttttaaaga cttttctgct ttgtctatgt caaaacaaaa ggagggatta
    61801 gcaatcttta actttctatt ctcaatcact acagcatctc cagctataac agcatctata
    61861 tcttttacat aaattgaaat atggcctggt gtatgacctg gtgtggatat tactttacat
    61921 tctttaaaaa attccaaaac ctgaccttca tttacttgat aatcaatttt gagtattatt
    61981 acatctgtca tttctaacct cgtttgtgaa aaatattata ccttatataa ctacaaacaa
    62041 atccatagac tgctttatta atatatgtat gatatgtata aaaagtaaat ttggtatata
    62101 tcagtaataa acagttttac tttcaggata aaagctattt tcttttatta atacaaaata
    62161 cattgaatat aagcccaaat aattcaaatt tttaatttaa tatttactac aacataataa
    62221 tattatataa tataagcaat tcaaggagga tataatgaat aagcgtgaat ttattaaggc
    62281 tatgtcagcc gaatcaggct tgacagtaaa agacacagat aaagcttacg atgcttttct
    62341 ggaggtagta gcaaaggccc ttaaagaggg cgacaaggtt cagcttgccg gattcggtac
    62401 tttcgaagta aagaaaagag ccgcaagaac tggtgtgaat cctaagacag gtgaaaaagt
    62461 acagattaaa gccagcaaag cacctgcact gagattcgga aaatcattta aagaattatt
    62521 ttaaaacttt aaaaatgaca gaaatacaaa cccggcaaaa cgggtttgtg ttttgctaga
    62581 tgcaaatata atttttatta tatatttatt tgtaatcttt ttttataccc tgttttctag
    62641 atatatcttt tacttttaag ttttcagtta aatgaaataa aaaaaagccc gcaaagttaa
    62701 ttaagtgcgg gttttaaata tggagctgac aagcagagtc gaactgccga cctcctcatt
    62761 accaatgagg tgctctacct cctgagctat gtcagcgact aggatattat aacatataat
    62821 attaatactt caatgctctt cttttaaaat attgcgtacc ttgttcttaa ctctctgtat
    62881 agcattatcg attgatttcg catctttttc aagagttttg cacataaaag ttattgtctg
    62941 gccttcaata aggaggtcaa aaacatcgct ctccatttta ctgagtcggc tgctaatttt
    63001 actcaagata tgctcaattg tttcttcctg tgtgattatg ttctcaggat tgaatttgtt
    63061 gtccggtatt gaaacagcgt actgtgtttc ctgttggttt tcatctgaat aaatggagat
    63121 atattcattt agcagtttgt gcttatacct gttagctcct ctgattgctg atatcatctg
    63181 cctgttgata cataatgatg caaaagtatc aaaagaagca tctttttctt cattataact
    63241 gataacagcc ttaaaaagac ctatcattcc ctcctgaagc aaatcgtctt tgtcggctcc
    63301 aattaaaaat aacccatttg ccctttttct gacaagataa gaatacttct tgagcaaaac
    63361 atccattgct atgacgtctc cattttttgc tttattaatt aactgctcta aagaattatc
    63421 tgtcatcatt cctcattctt aatagttttt ttttctgctc attatccaaa ttatctatta
    63481 tgaagttact attatttgta tttgcaatcc cattctttct tttagtaaat atctctgatc
    63541 tcaattcatt agcagtcatt cgtaaagcac ctttttgaaa aactgtcttc tgctcaagat
    63601 aatcatttgt aacaactgtt attttgtcag cataacgttg ctgtgtaaca tatctttcta
    63661 taaaattgtc agctgtctga ttactcattg taaagattac ttttatctta tcatgattta
    63721 ttaacgatcc ttttccttca ggtacaaggt gtgcatcaaa aacaataacg attcttaaat
    63781 ccgtatatcc ttggtaatct gataatagtt cacatagcat cttcctgtta tcttccaaag
    63841 gattttcttt gtcgaatata tctctccatg cattaataat attatatcca tctaccaaaa
    63901 ggtattccat agtttcttat gatccttgcc ttctttgcct taaagcttca aatatgacaa
    63961 ttgcagctgc acatgaagca tttaaacttg taatatttcc aacaagagga atatttactg
    64021 taaagtcaca attttgtgcc acaagatgcg acatgccctc tccttcagaa cctatgacaa
    64081 ttgctaatgg tccttttaag tctgctttgt tgtattcata atcagcattc tgatgagttc
    64141 cacatatcca taatccttcc ttcttcagta gctcaatagt gctgtttagg tttgttaccc
    64201 tagcaacctt tatgtgaaga agcgcgcctg cacttgcttt tactgctgca gctgtaacag
    64261 gagcagaccg atgcagtggt attattatcc catgacatcc tgctgcctca gcgcttcttg
    64321 ctatagcgcc taaattatgc acatcttcta tctggtctag tataactata aaaggcggtt
    64381 cattcttttc tttcgctaaa tcaaggatat cttcaacctc aataaattca attggagaaa
    64441 tatatgctac cactccctga tgtgatgaca taatagaatc caatttgctt gatgaaacct
    64501 gtttcacagg tatatttctt tttttagcct ctgataggat tcttaatata gagccttctt
    64561 tttctccact gcttactaat attctgctta taagatattc ttttttaatt gcttctaaaa
    64621 caggatttct tcctgcaatt acacttgtat tttcttccat tatatctcca acatgacccc
    64681 taaaaggtag tcaagccttt catattcttt atttacatat agatatccta taaggacctc
    64741 caaagcagta gcatatgtat agtctgtagc gtctgcattt ttgggtttgc ttttcgtttt
    64801 tgcatttcta cccctttgca cacagttaag ttctttagca tttaagatat ccataatttt
    64861 caacaggctt tgcttctggc ttttagcatt aacaagagat actgtcatct tgtgaagatc
    64921 tataggttta tagcatcctt ttacaataag atgttctcta acatataaat catagacagc
    64981 atcacctata tatgccaggc ttagaacagg catatccaga atatcattaa catgcaaatc
    65041 cttaaaaaac attaaaactc cttgctttgt atatattcaa ttatatcatg cgccattgga
    65101 gaatctgatt tctttgctga aatcgctttt atcatatctt gctttttaaa tcggacgtta
    65161 ttaaaacagt cctgaatact tgttataaat ggctcatcaa gagcatcaga aaatattctg
    65221 ctttgagtga ttattccgtt tttcaaacaa agatgaatct ctattcctcc ccaggaaaac
    65281 ctgtgtaccc attcaatatc aaatctgggc gcctgaccat atctatagtc ccaagaggaa
    65341 taaatatcat aaattctgtc tatttcctcc ttatttacaa atttctttaa actttgagga
    65401 ttatcaaact cattaactat ctcacaggtt ccgaattctt tctcgaatgc ctctataatg
    65461 ctaaatttaa ggtcatctac agtaatatca ttattatact cacgaagatt aacaatcctg
    65521 ctctgaacac tcttaattcc ttttgcttcc atcttctctt tacttacctg cagatatctg
    65581 ccaagttttt cacggtcaac atccaataaa actgtcccat ggtgtaaagc tctgtttctt
    65641 ctgaaagtaa aagcattacc tgaaaattta ccttcttcag ttactatatc attacgacca
    65701 ttcacatatg cttctattcc aaactttttt actgcttgta ttattattct tgcttgaagg
    65761 ttaaaatcct gactgcttct ttcaagaata aaagaaaaat tcaggttacc taaatcatga
    65821 tataccgctc cgcctcctgt tgaacgacgt gccagctttc catcttcgct ttccagaagt
    65881 tcagtcctgc actctctcca gggattctga ttcttgccaa taacgacagt attcgcattc
    65941 tgccatagaa acaggatatt gtcatattca gtgattttat ctaaaagata ttcttccaaa
    66001 gacagattat accaagggtt atgtgacttg ttaataacta taaggttctt catttttcac
    66061 cgcctttatc cttctttttg aatgagagtt tattttcagt acctgacaaa agtcttttaa
    66121 tattttttct gtgcataaat atggttaacg tagctaagaa ggccattgct aatataaagt
    66181 aaatttcatg gttaaaaacc catgccaaaa aaggcgctag aaaaacagct atcatagacc
    66241 ccaaagacac gaacctggtt aaaactacta taactataaa aactccaaga cctgtcaatg
    66301 caattctcca gtcagtcata agaagaacag ataacgaaat aagtattcct tttccaccct
    66361 taaaattaaa aaacactgga aatatgtgac ctacaattgt aaaaatacct gcacatatag
    66421 cacctgataa tccatttttt ataactatat aacctaatcc cgtagcaata actcccttaa
    66481 gaatatctgc tataagaacc atgactgcag ctctggttcc caacactctt aaggtattgg
    66541 ttaatcctgc atttttactt ccttctcctc ttatatcttt tttatacagc tttgaaacaa
    66601 gaatagccga attcattgaa ccgataagat atcctgaaat tgctaatatg atataaagaa
    66661 aaaccgtcaa ttatctttac ctctttctct gataataaac cttataggcg taccttcaaa
    66721 gtcaaatgta tctcttaatg tgtttgcaat ataccgctca tacgaaaaat gcataagatt
    66781 cttgtcatta acaaataaaa taaaagtagg gggttgtgta gaaacctgtg tcatataata
    66841 tattcttaaa agttttcctt tatcagaagg tggctgtacc attgctattg catcattaag
    66901 tacatcattt ataacacctg ttggtattct cctgcagctg ttttcataag ctttatctat
    66961 cattggaaat aagttatcca ctctctttcc tgtaagagaa gaaagtgata cgactggcgc
    67021 atattgcatg aaaggtagtt cttttctcac tgattgcaca taagataaaa atgtcttttc
    67081 atctttctct accaaatccc acttatttaa agccacaata attgctttac cctgttcatg
    67141 cgcaaagcct gctatctttg aatcctgttc tgatattcct tctattgaat caatcattat
    67201 gatacataca tcggctcttt caactgcaga ccatgctcta attatgctgt atttctcaat
    67261 gtctgaaaaa atcttgctgc tttttcttat acctgcagta tctataaatg tatatttttt
    67321 cccatctttt tcaatcaagg aatctatagc atctcttgtt gtccctgcta ccgggctaac
    67381 aatagctctt tcctcaccca ctatcctgtt aagtaacgaa gattttcctg aattaggttt
    67441 gcctactata gcaacattga taccgtcgtg ttcttcatat tcatccggtt cttcaggaaa
    67501 atgaacaaaa acctcgtcaa gcaattctcc tattcccaga ccatgaaggg ctgaaactgg
    67561 aaaaacttca gcaagtccaa gattataaaa ttcatataat tctgctggcg ggtcaccggg
    67621 tctgtcaact ttgtttactg caataactac cggttttccc gatttatgaa gaagattagc
    67681 aacatctctg tcccctgcag taagacctgt agttatatca gtcataagga taatgacatc
    67741 tgctgtatct attgctacct cagcctgcct gtgcatatgc ttgaatatct catcagaagc
    67801 cttagtttca atacctcctg tatcaataag tataaattcc ctgcctctcc agtcacattt
    67861 tatatatacc ctatctcttg taacaccagg ggtatcctgc actatggaaa tcctttttcc
    67921 agcaagataa ttaaaaaacg ctgattttcc tacattcggc ctgcccacta tggctactat
    67981 tggctttgtc aattgctctt ctgcctttca aataagttgg ataaataata ccatacccta
    68041 gtaatataaa caagaatacg gctgttttga ttttatgttt actttacaag cgcttcaata
    68101 aactgagcgc cattgttatt aactactgtt atagttgtct ttaattcttt tgatatttgc
    68161 ttaagtgaat aattgtctaa aaatatatct tcatcagctt taagcatagt tcttgagata
    68221 agcaacccat cttttaaatc aaccttcttt aaaccatcaa gcagatcctg tcctgtaagt
    68281 aagcctgtaa cggtgacatg gcctccaaag aaattgtttt caagtttata aacatcaatt
    68341 ttaacatcag gagcatattt cataacctct tttactaatt gttctataaa tgaaaaagag
    68401 agtactgagg tagcaacatt aatatgtttt ttcttctttt tcagtctttt caattctttt
    68461 ttatttaagg acaattggtc aaggtactct tttgcatcat atatgaactc tctcatcata
    68521 ccaacaccat tttcaatctg ataaaagcca tcatattcac aaaaatcggg gatatcaagg
    68581 cctgctttta tataaaattc atcagctgca tatataagat tatatccagc cttttcttta
    68641 aattttttct gataaccctc aatttgtttt attatatctn nnnnnnnnnn nnnnnnnnnn
    68701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    68761 nnnnnnnnnn nnnnnnnnna catctcagtt tcggatcagt tgtgtgtacg gaaacattaa
    68821 ctggtgaaaa atgcattgaa gcaatcctgg aaatatcatc atatgacata tttgtcaaag
    68881 taacataact gccggtcaga aaggaaagcc ttgcatcatc atctttgaaa tataaagatt
    68941 ctctcattcc ttttggaagc tggtcaataa aacagaatat gcatttatta tgacaggatt
    69001 tctcgtcatc tattaattct ttttcaaaaa ccagtccaag atcatcgtca atgtcttttt
    69061 ctatttccaa tttaacaagt ttccctgttt tctgcttgat aacttccaaa gttatgtatt
    69121 catttgaaat ctgatattga taatcaaata tatccttaat tgaaacatca tttacagaaa
    69181 gcaaaaaatc accggatttt attccggcct tttttgctaa acttctgttc tgaacttgtt
    69241 ttatagcgat tgcttttttt tccatatagc aaaaaggcga tatgtacatc gccttataat
    69301 attacttttc cgcttcttgt tttataactt ctttcccatc ataatatcca cattcaggac
    69361 atactctgtg ctgacgctta agagtatggc attgtgagca tcttaccaga ttaggcgttt
    69421 caagtttata aaaagttctt cttttacgtt ttctttgttt tgatacttta ctttttggta
    69481 cggccatttt acacctcctg tattattagc attattcttt aattaaatcc cttaaagccg
    69541 caaatttatt gttttcttgg ctttcttctt tgcatttgca ttctttttcg ttaagatcgc
    69601 atccgcaatt cgggcaaagt cccttacaat caggtacaca taataccctg tcagggatgt
    69661 ttagaaatat ggaatcaatt accgcttggg atatatccag gatacgtcct gagtattcat
    69721 agttatccaa gttgtctgaa ctgttcgctg gttcaaaaat ctcttccatt tctatagtaa
    69781 acggttttac tatctgcttt agacatctgt aacatttcgc atcaagttgc ccagtgattt
    69841 ctgcagttac agtcaggatg ccctcattgt tacatatgtc accttttagg tgtatatcac
    69901 ctaaaaatcc gactaaggtc atgccaccgc cagttttgtg gctgtcaaca tccatttcga
    69961 actttttagc agttccgtct ctttctatta tatcggttat gtcaattttc atttagtacc
    70021 tttcaaaaaa accgacaagt gctattatat ggtggcagtt ttaccttgtc aagacatatt
    70081 tgttttagtt gactgcttca agcaaacttt tgcactgtct ggctatctct aactcttcat
    70141 ttgcaggtat aatcagtatc cttacattag atctgtcttt actgacatct gcctcgccct
    70201 tggctgcatt attctttttt ttgtccattt taattccgaa tatatccaaa ttattggtta
    70261 ttgcttctct tatataagcg ttattctcgc caacacctgc agtaaatata agtgcatcta
    70321 ttccacccat aacaccaaca tattcacata tatacttttt gactctgtac gcaaaaatat
    70381 tcaaagcaag ctgtgctctt ttatgtcctt catctttcgc tttgattaaa tctctaaaat
    70441 cactactgat tccggaaacc cctaatatgc cagaattttt atttaagtaa tcatttacct
    70501 cttcaacaga catatgcttt ttgtccatta tatatgaaat aatggatggg tcaattgttc
    70561 cgcttcttgt gcccatagct aagcctgcca atggagtaaa tcccattgat gtgtctattg
    70621 acattccttt atctatagcg catatagaag ctccgcttcc taagtgacag gaaacaatct
    70681 taattttttc tagcggtttt tgcataattt cagcagctct tctagaaaca tatctgtgat
    70741 ttgttccatg aaaaccatat ttacgtacgc catacttctc atacatttca tatggtaaag
    70801 catacatata tgcatagtca ggtattgtct gatgaaaggc tgtatcaaaa acagctacca
    70861 ttggaacatc ttttagtatt tcttgacaaa ctcttatccc tgatatgcca gcagggttat
    70921 gtaatggagc taaatctatg cagtcttcta ttattttaat tacgctatcg tctataagaa
    70981 caggtttgtt gaatttctcc ccgccatgca ctactctgtg gcctacagca tctatctcac
    71041 taatatcttt aagaacccca tattcatcag atatcaaagc ttccagcatt attttgaaag
    71101 catatgcgtg gtctcttatg acacattcat ttacaaatga tttgttttca gagttagtat
    71161 aagaatggat accgtttaca agcccaattc tttcacataa accctttgca agcattctct
    71221 cattactgct atcaattaac tgatacttta atgatgaact gccggtatta actactagaa
    71281 ctttcatttt ccccctagcc ttgctgtata aatttacgca aactgcgctt gtacagctgt
    71341 tatggcaata acccccacta tgtcttctgc agaacatcct cttgaaagat catttacagg
    71401 ctttgctatt ccctgcaaaa gaggtccgta tgcttcagct tttgctaatc tttgcgttat
    71461 cttatatgaa atgtttcccg catcaagatt tggaaatatc attacgttag ctttcccagc
    71521 aacttcacta ccaggtgctt tcttctgccc tatttctttt actacagcag catcaacctg
    71581 aagttctcca tcaagtaata aaccgggtgc tttttccttt gctaatttag ttgcttcaat
    71641 aactttatct gttaattctg atttcgcact tccataggtg gaataactta acattgcaac
    71701 tacgggtttt tcctcaacaa gcatttcaaa agattttgat gtagatatcg ctatttcaga
    71761 aagctggtct gcgtcaggat tctcgacaag tccgcagtct ccgtatgcaa atacaccgtt
    71821 tgctccatat tcgcagtcag gtacgcacat tataaagaat gcgcttacta tctttgttcc
    71881 ttttgctgtt ttcaaaatct gtaaagccgg tcttaatgta ttacttgtag cattcactgc
    71941 tcctgccacc attccgtcag catcacccat cttaaccatc ataactgcaa aatacaaagg
    72001 gttttccaat aatttatatg ctatttccaa tgttacgcct ttttcttttc ttaactgata
    72061 taaagcatta gcatattcgt tcattttttc aaatttatct atttcaacta tcttatcttc
    72121 agggatatac aaacctttag catctttcat aatctgttct ttattaccta atagtattat
    72181 gtcagcaata ccttctttaa gtactgttga tgccgctttc aaagttctta tatcttctga
    72241 ttcaggaagc actatggttt ttatgtttgt tttagctctt tgaatagttc tttctataaa
    72301 tgtcatatat ccacctcata aataagtatt taactaatac attatagtcc cgttacaaca
    72361 tatattcaag tataacgggc aaaaagaaag gagctctttg tgagtttatc aattatctct
    72421 ttagcaagct cctttttttg tttattgttc tgtctgataa cttaggtaaa gatcctgata
    72481 cagcctgtag tgcataacag catatttact cattgcttta tattcctgca tctgaagctc
    72541 ataatcactg cttgatataa gtctgtaaac atcatctttt acagtatcaa attccgttct
    72601 gtctattaac ttgattacca tgtaccctat ttcggtttct aatagtgtaa tatcgccttc
    72661 agttgcttca aaagcccaat cagcaagatc tgttacacga acctcattct ttgtaaatgt
    72721 atattcacca ggctttgtag ttgatgtttc ttcttcctgg tctgcttctt cgtcttctac
    72781 ggaatcaaca agatcatcgc tgtaaagttc agcaagctca tatatatcct gtcctccgag
    72841 tgcaagctgg taaacttctt cagcttttat tttagctgca gcaacaacat catcagacaa
    72901 aggattaagc tgctcatcaa cggtacgctt caatactgtt acaacagtaa ctttatcaat
    72961 atcatctttg atcttatttc cttctccatc aactgattca taataagctc ttgctctgtc
    73021 ttcagtaaca gcactaagat tctgctcctc aatataactg gaatactgct gaactttgag
    73081 agtctgcttt attacttttt ccatagtttt gaaagtggtt ccataatata tttttgcata
    73141 atcatccaaa tcataaccag cttcatctgc tgctttttga atgtttgtaa gctgtatgtt
    73201 atagctttct tccagttcct gttcatctat ttgaaaacct gtttcttcaa ttaaatccaa
    73261 aataatatat tgttcctgta tagaatccac aacattctca agaagcatct gtccaaatga
    73321 acgctgatta tcaaatgctg tctttaaaaa gttcaataca ccatcctgtg tagtaatgtc
    73381 aaaacccaaa cctgcgatgt aatcctgata gtagtttaca aatacattat attgcgtatt
    73441 cgttttatac atgaattctt cttctgcaat attgctattg ccaactttaa catagttctt
    73501 tggtattaag cttgccacta caaaatatat tatacaaaga gcgacaatta caccaagtcc
    73561 aagaaatcca tatcttataa tggtttcttc ctttgatatc tcttttgtat tttttttact
    73621 gtcttttttt gcggtatttt cctttatgcc tttagttact gccatttcat atctcctttt
    73681 tgatatcaga tataattaac tggtttaccg aatactaatt ctacaatatc cccttactct
    73741 ttatcaagtc ttattttatt tcatatgcat cttttacttc taatttcgta tttattaata
    73801 cattttccat taattttata acaaattcaa gaagagcttc atgtttttta ttagtttcaa
    73861 ccctgattct taaaaaatca tatgttttaa ctgagtacct gcattttaac acatccagta
    73921 catttattac atctccaaat ctgaatgctt cttttttatc ataaattata tcaaatcctt
    73981 ttttattctg tttgatttca gatatacccg cttttgatgc aagatttttt gtatatgcaa
    74041 catcaattat atttaataca tgaccaggcg gttcagaata tctgtctata agttcatcaa
    74101 taatatcctg tttttcagat acagattcta ctagcgcaat ccttttatac attttgagtc
    74161 ttgataaatc atcctgaata tatgacttat ctatgtatgc gcttatattt aaatcaatac
    74221 ttgtttcaac cagggcatcc ttgactttct cacctttcat tcgtgcaatt tcctcagaaa
    74281 gtatccttac gtacatatca tatccaacac cctgcatgaa ccctgactga tttgctccta
    74341 gaaggtcacc gcctcctctt atctcaagat ccttcattgc aatcttaata cctgaaccga
    74401 actctgtata ttctttaatc gtctgaagtc tttttgatgc ctcttcagtc agctctttac
    74461 cccgtttata tgttatatat gcatacgcta ctctttcaga gcgacctacc cttcctttaa
    74521 tctggtaaag ctgtgcaaga ccaagtctgt ctgcatcttc aattataaga gtatttgcat
    74581 ttggtatatc tatccctgct tcaattattg tagtacacaa aagtaaatca tattcatgat
    74641 ttataaatct gattattgta tcttcaaatt gtttttcctg catctgtccg tgagctatac
    74701 atatcctgac atcatctttt agcatgtttt gaagctgata aaacttattg tccatatcca
    74761 taactctgtt atacagataa aatacctgtc caccccggtc cttttcacgc aatattgctt
    74821 ctttaatcat ttcagggtca tattcaagca catatgtctg aacaggaaat cttttttcag
    74881 gaggatcata aatagtgctg acatctctga ttccagacaa ggccatatgc attgttcttg
    74941 gtatcggagt tgcagtaaga gacagaacat caattgtagg tttcaatgtc tttatttttt
    75001 ctttctgagc aacaccaaaa cgctgttctt catcaacaac taaaagacct aaatccttga
    75061 agataacatc cttctctaat aacctgtgag tacctactaa tatatcaacc ttacctttta
    75121 gaaggtctga aatagttttg ctctgtcttt ttttattcct cagtctgcat aagaggtcaa
    75181 tattaacagg ataattcttc attctttcaa caaaattatt gtaatgctgt aaagcaagca
    75241 tggtagtagg agcaagaaaa gcaacttgtt tcccatccat cactgcttta aacacagctc
    75301 ttaatgcaac ttcagtctta ccatatccga catcaccaca taaaagcctg tccattacct
    75361 ttgcagattc catatcttct tttatctctt caacacatct tgtctgatca tcagtctctt
    75421 cgtattcaaa ttcattttca aatgatatct gccagggagt atcctgcaaa aagatatgtc
    75481 cttttacagc agttcttctg ctatacagct ctattagctg ttttgctata tctctgacgg
    75541 cttttcgaac tttattcttc tgtttttccc attcttttcc gccaagacct gtaattcttg
    75601 gagttttctc tcctgaaccc acatattttt gtataagatc catcttgctt acaggaacat
    75661 aaagaacatc ttcatttctg tatgcaatct taatatagtc tttaataata ccttcaattt
    75721 caattttctc tactccctga tatataccga taccatgata atcatgtaca acataatcgt
    75781 taggctgaat ctgggcaaag aaatcatcat ctcttatctt ttttggcttt tgtttagcat
    75841 ttttcctgaa tatcctgctt acagatatgc ttactatttt tgcttctttt gagacaaaac
    75901 tttcattaaa accatcttct agaatatata cgctcttagg ttgtattgca tattcttggc
    75961 ctttatcaat tatatgtgta ctaaaaccat tgtcttcaaa aaagccttta aggtattctg
    76021 tctgctttct tcggtctgaa ctgataaaaa tatacatatc ctgatttttg tattcataaa
    76081 agtcttccaa aaacagttcc atgttccctc tatatgatgc aggctggtcg aatgatatgt
    76141 caaaatccct atataaaggt atattgtaat cagatttatc atataccagt actaaatcag
    76201 gttttaaaaa tgaatacagt gagtatatgt ccttctgcat tacacatgcc tgtgtcaatg
    76261 ttttcttctt ctgtaataaa ttcacacatg catttgcata ctctttatac actgtttctg
    76321 caacagcatg acatcttaca tcattatgaa caacagtaag aacattcatg tctttaaaat
    76381 actctgctgt cataacgcat tttcccaata ttgcaaagta tctatccata atttttgagt
    76441 attcgccatt ttgaagtttc tgaagatccc agttatcagt tatcttttct gaaatctgat
    76501 cttcttcatc tcttgtaatt aaaaattcac aaaaaggaat tatagtagtt tctcccacat
    76561 catctatgga cctttgtgtc tgagcatcaa agaaacgcaa actatcaatt tcatcaccga
    76621 aaaattcaca tcttacagga ttatcgtaat ttggtgaata tatatcaaca atcccacctc
    76681 ttacactgaa ctgtccctgc tcttctgtca aagctcttct tgaataacct aattcagata
    76741 acttatcaca taatgcctgc atatcatact cataaccttt ttttagctgt atcaggtggt
    76801 ttatgaatat ctcttttgaa gttgtgccta cagttaaagc ttcagtacag gcaactactg
    76861 cctggaaatc ttgtgtagca attctgaaca aggcattgat tcttgcctgc ttctcattac
    76921 tgctgtaagc atctatgtca aacagcataa gctctcgcac aggaagatat actacatcat
    76981 ctccatatag tgaaatgagg ttctggtata tggatattgc tgaagttctg gaatgcgtta
    77041 ttattagcag ttttcttgac accatttttg cggttacata ggaaactaac gggcataaag
    77101 aattccccgg tccggacaat cctacggggg tctgtttcat cactatgcaa tctgctatcc
    77161 ctttttgctg aggaatttca gaaaatactt gtacaaaagg attcatttaa ctacacatca
    77221 cctttatctt tcttgctatt gtatttattc attgcaatat ctattccttc agataatatg
    77281 caagttatag ctttaactgc tgtatccaga gcctgagata ttatctgttt ttcctgcttt
    77341 ggaaatctcc caagaacata attcactgca tcttcattca gttctctgcc tatgcctatc
    77401 cttatcctta tgaactgatt tgtccctaaa tgattaagaa tggacttcat gccattatga
    77461 gtcccgccac ttcctttata ccttatacga acatcaccta agggcaagtc catatcatca
    77521 tatattacaa tgagcctgga tatatcggat ttataccagt ccataagagc tcttatgctc
    77581 tgtccgctta agttcatgta tgttctcggt ttggcaagaa caacaggatg gtttttaatt
    77641 ataccctctc caacattagc aaggcaatga ttttttttaa cctttatgtt aaatatatct
    77701 gataattcat ctataaccat aaaccctata ttatgtcggg tattcctata cttcagtcct
    77761 atgtttccca atcctgttat tacatatcta tccattattt ttctgtcccg attgaaggag
    77821 gaaatttgaa taaagctgac atggatgtat ttgtatatat cttatatatc gcttctgaga
    77881 acagttttgc aacttcaaca atttctattt tatctatcat ttttgattct ggtataggaa
    77941 cggtatctag aagatacatt ttttctatac ttgaatcttt tattttctct acagcatcag
    78001 cagacaaaat accatgagta gcatacacaa aaactttttc tgcacccatt tcctttacta
    78061 cattagcagc ttttgttatt gtaccggctg tatcaatcag atcatccata ataatggcgg
    78121 tcttcccttt tatgtcaccg ataatattca ttatttcaca ctcattggct ctggttcttc
    78181 ttttatctgt aatcgctatg ggaagttctg taagatctgc catttttctt atgcgattaa
    78241 ccgatccggt atcaggtgca acaagaataa aatctgactt atcttttcct ttcatcaagt
    78301 caagaaccat gtcagaaaag attattgaac cttccagatg atctacaggg atgtcaaaaa
    78361 atccttgtat ctgtggagca tgtaaatcca ttgttagtat tctgtccacc cctgctgttg
    78421 taagaagatt tgccacaagt ttagctgaaa tcggatctct tgcacgagct tttctgtctt
    78481 gccttgcata tccgaaatat ggcattacag ctgttactct tgcacttgat gaccttttca
    78541 aggcatctgt cattatcagt aattccataa gatgctcatt caccggagga caggttgact
    78601 ggattataaa aacatctgat cctctgacaa gctcatttat aagcatttgg atttctccat
    78661 cagcaaatct gccgactttt gattccaaaa gcggaacacc tagataattt gctatctggg
    78721 ctgcaagttt aagattagca ttgccaccca ttattttgat gtcattctta gccaaaacac
    78781 attccccctt atgatatgat ctgatcatat ttatttcatt cttcctgttt tttctaccca
    78841 gccttcttta ttgacctggt gggctcttgc tattgcaagt gttcctgttt ttacgtcatc
    78901 tactatggtc gaacctgcag caatgaaagt gttatcttct aattctatcg gagctactaa
    78961 tgtactattg caccctacaa atacattatc acctatcttt gtcttgtatt ttgccttgcc
    79021 atcataattt gcagtaataa caccgcatga tatgttacat gcttttccta tctctgaatc
    79081 tcctgcatat acgaaatgag gaattttggt attgttatca actgtcgtat tcttaagttc
    79141 tacaaaatcc ccaactttta ctttatcttt aatgcgtgta tggggcctta tataagcaaa
    79201 tggaccaatc gtgcagttct gtccgattga actttcaaga acaactgatt ttataactga
    79261 agtacctgct tctactctgg atgactcaat ctttgtgtca ggacctatga tacagtcttc
    79321 gcttatatat gagtcaccct ctataaagca gtttggatat ataacagctt ttggagctat
    79381 tttaactgta taatcaatat atgtttccgt aactgaaagc attctgactc cattttcaat
    79441 gtgtccgata tttatctgtg tttttacatg ctcgtaagct tcgtaatatg atattgggtc
    79501 atccacaggt tcaaattcca gctttccctt ttttttcata tactgaccaa taaaacttac
    79561 aggacagtag aatatatctt ctggaacaga taaaccgctt tcatcataat agtatccaag
    79621 gctgaatagt ttatcttcca ggatttcctt cggagtcact ccgaatacca tgttttctct
    79681 gtatgcagag aaatactcca tttctttaat agtaaatctt ttatcatcca ttttctcatc
    79741 atctccaatt aaattataca acaatgatta gcaaattaac accatttggc tactattttc
    79801 ttgatttgac ttatcattta tatgcttact tcaattactg ctgtagtacc agttgcaatt
    79861 tcaatatcta catatttttt atccctttta ttaatgattt tgcagccaga attggagata
    79921 tctgaaaaac ccatcggaat ttcaagagta agaatactgc ttttaatagg gtttaatgta
    79981 actgttatgc gtttttttac aatgtcccac ttaatatcca cagaacatgc acatctggtt
    80041 tttattcctt ttactgaacc tttttcccat ttctcatcta gagcaggaag aatccttata
    80101 tacgcaggct tggaaaacac aagcatttcc aatactgcag cggtaaatcc gaaatttgca
    80161 tcaatctgaa atggcggatt tcctccaaac cagtcagcag tcacaccctg tcctctccag
    80221 tcattgtgga aggtgaaaag attggattta acacaggatt ttataagatt tgaaagacac
    80281 atagaagctt tatatccatt ctccaaacgg gcatatatat ttgccatatg tgcatatgac
    80341 catcccgtct gttcgctaaa acctactagc agcctcttgt ctacagcttt tttaattgct
    80401 tcataatatt caagtgatgt ctgctttgtt atttcatatc caggaaatag aggataaatg
    80461 tgggactgat gtctgtgata gtaattgtct ttcaagtcct catgaagcca ttctttaaaa
    80521 gctccatcct cgttaatcat atattctggc attttactaa gcatatcctg ccatttttct
    80581 gaatcatcat ctgtaatgtc tagatgctta tatgcataaa ttaaatttgt caaaacttct
    80641 tttgctattg caacatccat agtagcatta acacatatcg atgatctgtt ttcgcagacc
    80701 ggtgtgtttt caggcgaata tgatggtacg aatacatatg tgccagagtt atccataata
    80761 agataatctt cataaaactc cactacattc ttcataaacg gtatcgctct gtttactaaa
    80821 aaatcatcat ctgcagtata aagccagtaa tcaaaatagt gctgtgcaat ccagccgctt
    80881 gctgaaatcc aatgagaatt gaactttcca tcggttgtct gcgcaagagg aagcagtatg
    80941 cctttagctc catatactct ttttgcattt tgcctgtaat cttctatgaa ctgctcgaaa
    81001 tagtcataaa aaggcattgt gcaatcgttt agtccacaag aaagcgctgc ccagtaattc
    81061 atctgaatat tttcatcaag atgaaaatct gaagaccatg caggattata gtctccattc
    81121 caaataccct gaagattagc tggcaatccg ccttttttag aagaggatat gagaagatat
    81181 cttccataat tgaaaagtgt atgtattatc tcctttgaag ctttttggtt atatgcttcc
    81241 ttgagaagtt gttcgttgct tttagctttc ctgctttgtc caatatctaa agcaacactt
    81301 ccatagagct gtttccattt ttttgtatga gatcttttta atttttcata atcgcatttt
    81361 aacagctgct tttttattct gctgactgct ttattgtcat cttcattaac atatgttttt
    81421 acgaaaatat aggttttgtc agatttcctg attcgtatat tcttctcatc atgagttttt
    81481 atcccattta ttcctttaat atacgctacc gctccatggt tagacccatt aggatattta
    81541 actttgtaaa ccagaaaatc atcatcagta tccaaagtat attccataac gcctttagat
    81601 tcagaaccac cttcaatgtt tactcttgct gttatataac cttcttcaga agcttctata
    81661 cacacacata ttacatcctc atcatatgaa ataaatgttg atctttttag taaaacttca
    81721 tcttctttcc atgatatttc tgctagtgct ttcttaaaat ccagtttaaa gatataatca
    81781 gaaaaaactt ttttgatttc ctggcctata tacataaaac ctaaaggctg atatggatca
    81841 ataccctttt tgttttcata tgaatcatca acactcttct tatataactg ttcagctttt
    81901 gaatactgac cttcaagtat cagtttccga ataacttcca tattatgact tatagcccct
    81961 atttcaacag gttcagactt attgtaaagt ctttcatggt tcaatactat cttttctcta
    82021 aatatacctc caaaaagcaa agctcctatt ttgccatttc ccaacggaac agcttcatcc
    82081 cattttgcga ctggcatttc tttaatcata tagtctgaca tacatcctcc tacttaacag
    82141 ttattatgca tttttcgtct tttttgagct ctacactgta gtaactgtaa ttattctgca
    82201 aatctgtatt tattaattca atatttttac aagtgctgtt aatgttattt ggaataacgg
    82261 gaagctgaat ggttattttt tggtcttcca gtgaaaaaat tgctatttca atatctgact
    82321 tatcccatga tatatcacat tttgtacgag ttctggtatg tatacctttt attctgcctt
    82381 tcttccattg gtcaggcaaa gctggcagta ttcttaagaa tcctggtttt gagaaaagca
    82441 tcatttccag tacagcagca gtaaatccaa gattagcatc tatctgaaat ggaggatgct
    82501 gaccaaacca gtaaactgtc aatccctggt cgcgccaatc attatggtaa gtgaaaaggt
    82561 tacttcccgt acatgctctt aagatatagt tcaaacaggc atatgcgtta tctttatctt
    82621 ccagtctggc atatatatta gccatatgcg catatgacca tcctgtctgc tgaacaaatc
    82681 caatagttag tcttttatca actgcagtct ttattgcatt atatattttc ccattactct
    82741 cttttgtaat ttcaaatcct ggaaaaagag gatatatatg agactgatgc ctatggtaat
    82801 agttatcctt tatatcatca tacagccatt ctttcattgc gccatcttcg tttacttcgt
    82861 aatcaggcat cttatccaac attgcttccc atcttgcaag attctcctgc tctatatcga
    82921 gatatctaca ggcagttatt agattggtaa gagactgttt tgctactgca atatccattg
    82981 tcgcatttac acacatcatt gacgcatgtt caacattagg aacattttca ggcgataatg
    83041 aaggaacaaa aatatacttt ccgtcctctc cggttattaa aaaatcttca taaaacagca
    83101 cgacctcttt cataaacctc acagctctgt gctttagaaa atcatcatct ttggtataca
    83161 gccagtaatc ataatagtga gaactaagcc agcctcccgc tgaaatccaa gactgccatc
    83221 tgcctgtcat tccgcttatc tttccatgag tgctcatgca tattggcaca agaattcctc
    83281 tgcacccata tatcttttta gcattttcac gaaaatgagg aataagcgaa tcaaaataat
    83341 cataaagggc catagatgaa tcaaactgcc ccaaggcaag acttgcccag taacacattt
    83401 gtacattctc atcattatgt atatcagaat cccatttagg attatatgtt ccattccaga
    83461 ttccctgaag attcacaggc agtccgttct ttctagaaga ggatataaaa aggtattttc
    83521 catagttaaa catagtgtga aacaagttta acggtttttc atcattatat gccttaagaa
    83581 gatattcctc atttgaaaga ttcttattat ccgaatagag atctagatcg gtactgccat
    83641 agagcttttt ccatgtgcta atatgctttt tcttaagctg gttatattca tattttttaa
    83701 tctgttttat aagacgatta acagctttat ctgcatcttc atatatatac agcatcgtat
    83761 atatgtttat aacttttgct ttttcaacaa taatagtatc tttagcaaag tcaacatttc
    83821 cacctgttgt ttccacatat ataactgccc catgctccct tttgtctttg tatcttgcct
    83881 catatattaa gcaatcattc ttaatctcac atttcgaatc catatactca tcaccaccgt
    83941 caacctggtc aacattcacc tttatactca gcagtttatc atccgatgaa tacattgatg
    84001 tacatatgca tcctgtatcg tatgaaacaa aactttctcg tacaatcttt gtattcttct
    84061 ccttccagct gacggtcgct atggctgact ggaaatccag tacagcttta taatctgcaa
    84121 atgcatcatc aggttttctg aatatagaaa gctcagcaaa tggctgataa gggtcaacat
    84181 tgtatacttc cttgcagcat tctttaaatt tttcgttaaa gtatttttca ccctcgtcat
    84241 atttttcatc taacataagt tttctaacat cattaagatg cttactgaac ggatcatata
    84301 taggctcttt ttgataacga aagagtcttt catggttaat taatattctt tctttttgga
    84361 tgtttccgta aatcatggca ccgactgtgc cgtttcctaa aggtaaagct tcctgccagt
    84421 ctactgcggg caatttctta atcatataat cagacatatc acaccctccc ttaggatatg
    84481 ataccacagt tatacatcac tttattatga ttatgattat gattttgatt ttaattctat
    84541 cattctttcc atatacatag agatgtctca gaatcatctg caaaaccaaa gtgtggcatc
    84601 ccgtatttat atgtttcaat tctgcttagc atatattcct caaactcact tactgtaata
    84661 tgattattca taagttcaat aaatgtatct gctcttgcag cattctttct tatatgaaaa
    84721 agcttgtctg ctgctataag aatatctttt tcactttgac taagactctt ttccgatgaa
    84781 accagtttat ctctgcatag cgcatcaggg taaataaaat ttaaactgat tagttgctct
    84841 tcattcacta tctcatacga tgaaaaattt attggttctt ctttatctga ttctgaaaga
    84901 taaaaatcat atcttgtttc tggattgcta accgatgtta aaaaacttgt tttacaaatt
    84961 gatattccat acaattcttc aaacatacta tttacatgct gaatatcatt actttcttca
    85021 ttcatatttc tactgtttac ttcccagaat ccataaaaca gatcttcttc gaaaaattct
    85081 tttcttacga tatatttatc atttttcttc agtacaacag aaattttaac catttcgcat
    85141 ctcctttttg tcatataaat acttgcagct tgattctaat atctcctctt ttgttttctt
    85201 tctctttgaa atatgcatta atacaatatc atcacctaac atgaaatctg ctttatcaag
    85261 atctgcttct tctatccaaa gtaaattttc aggcatttct ccttttacaa tacatgcttc
    85321 aaaatatgca tagcttataa catctaaacc gtctttgaag tcttcttgat atatacattt
    85381 ccctactgat atatcaaatt taaatttttc ttttatacca tatgacagta tatcttgttt
    85441 tcctgcaata gcattaatgg tttccactct tggaaacata acatacatat gaccattcat
    85501 ctcaatcgtt tcaaccagat atctgctatc ttttttaatt actgcaccaa gaaattctgc
    85561 taacataaac actccttttg atattattta gaaattcata ctaacatcaa aaggagttgt
    85621 gtcaatagac aattgacatt tattatcaat attatatata tttactagaa tagtcctgtt
    85681 atcttgcctt cattatcaat atcaattgat tcagctgcag gcacttttgg taaaccaggc
    85741 attgtcatga tttctccagt gagagctaca ataaaccctg ctccagctga tattttcaaa
    85801 tccctgactg ttattacaaa atcagaaggc ctgcatatca gtttggtatt gtcagaaaat
    85861 gagaactgcg tcttagcgac acatactggc agattatcat atcccttttc tcttattact
    85921 ttcagcatag atttagcttc agaagaaaat tcaacgccat ctgccccata tattttcgtt
    85981 gcaatagctt ttattttttc agttatagtc atatcacttt cataacagta ctcaaattca
    86041 ttctgcttat cacataactc aactactttc tcagccagtt caattccacc ttctccgcct
    86101 tttccccaga caaggcattc tactgcagtt acaccataag attcacagcc ttttttaatt
    86161 acttctattt cttccaaagt gtcattcgag aatttattaa tagctacaac tgcaggaact
    86221 ttgaatactt ttgttatgtt ttctatatgc ttatataaat tgtccatgcc gtctttaaca
    86281 gcctgaacat tggtatcacc aagttttttc ttattaaccc ctccatgata tttcaaagct
    86341 ttcacagtag ctacaataac tacggcattg ggttttaatt ctgccattct gcatttgata
    86401 tcaaaaaatt tctcagcacc taaatcagca ccaaaacctg cttcggtaac cacataatca
    86461 ctaagcttca aagcgtattt agttgccatc acactgttgc acccatgtgc aatatttgca
    86521 aatggaccac cgtgtatgaa tgctggtgtt ccctcaagag tctgaacaag attcggctta
    86581 atggtatcct taagaagtgc tgcaagagca ccttgcgctt ttaacataga agctgtaaca
    86641 ggttcattgt cataactgta cccaattatt atattaccta ttctttgttt caagtcatca
    86701 atactttgtg ataagcacaa aattgccatt atctcacttg ctactgatat atcaaaccca
    86761 tcttctctgg gcattccgtt tgtcttacct ccaagaccgt caacagtata tctaagctgt
    86821 ctgtcattca tgtctagaca tcttttccac gttatacgcc ttgggtctat acctagcaga
    86881 tttccctgat atatatgatt atcaatcaaa gcggccagaa gattatttgc agcagttaca
    86941 gcgtgtatgt cacctgtaaa atgaaggttg atatcctcca ttggtattac ttgtgcatat
    87001 cctccgcctg cagcccctcc tttaacaccg aaaacaggtc ctaacgaagg ttctctaaga
    87061 caaagcattg attttttatc aattcttctt aacccgtctg caagacctat actggttgtg
    87121 gtttttccct ctccagctgg tgttggggtt attgcagtta caagaataag tttaccatct
    87181 ttgtctcttc cctgtactgc cttacttgta actttggcct tatatgggcc ataaagttca
    87241 aggcattctt ctgcaatccc tatagaatca gctatttgtt taattggttt tattaatgca
    87301 ctttgagcaa tttcgatatc tgtaagcata tggcaccttc ctttatttga attttgacat
    87361 aaaataatta tacatcaaaa tacatatatt gtggttataa aatgccaaat tcgtccttat
    87421 atacatctat actcatgttg acggattttg gaagtttctt catcatcagc atatatgctt
    87481 ttgtgagatt ttcctcaaat tgcttggtgt tagtgtgttt tactattatt cttgtacggt
    87541 acttatcgtt cagtttgctt atttggggtt tagagggcga atataccacc atatttgttt
    87601 ttttcagtag attatatgct ttttcggcat attcctcact tagatgctgc tgaatagagg
    87661 agaagattat aaatgccata ttagtatatg gaggaaattc taaaaccttc ctgattttca
    87721 tttctgattt ataaaaatta tcataatcct gctctatggc gcttaccatt aatgcagaat
    87781 ccacattata tgcctgtatt atcgcacgtc cctttaagtc tgatctgcct gctcttcctg
    87841 ctgactgcgt tataagctgg tatgcattct cattagattc ataaaaagat cccgctccta
    87901 gagcatcagc attgaggatt ccaactaaag taaccaaagg ataatcatgt cctttagcta
    87961 tcatctgagt tccaatcatt atatcaatct tatctttttc aaatctttcc aatatctcct
    88021 tatgagagtt tttcattcca gttgtatcaa gatccatcct gataactgat gcatctggaa
    88081 atagttcttt taattcagct tcgcatttct cggtaccaac accgcactcc atcaaagtct
    88141 gttcattaca attaggacat ataatcggtt tcttctgaat atgtccgcag taatggcaaa
    88201 taagataatc tctgccttta tgataagtca taggaacatc acagtgttca cattttatta
    88261 tatgtttaca tgatgtacaa agctgcattg aggtgtatcc tcttttatta agaaaaagca
    88321 cagtctgttc atgattatca atgttctttt taatttgatc tatcaatgta ttactgaata
    88381 cgctcatttt tccatttata gcatcttctc tcatgtcaac tattgaaact tcaggtaatc
    88441 tgccagatgc tctgttttgc atgaataaaa ctctgccaat tctttcaaac gcatggaatg
    88501 tcgtaacttt tggagttgca gaacctaaca caagaactgc gttttccatt tttgccctat
    88561 tatctgcaac aaaattcgta tgatatttcg gagtgctttc agatatatat gaattgtcat
    88621 gctcttcgtc aataataata agtccgagat tactaaaagg agcaaatata cagcttcttg
    88681 tacccagtgc aatctttact tctcctcttt taattctcat ccatgcatta tatctttctc
    88741 tcatggaaag cctgctgtgc atgacgacaa ggttattacc aaatctttca gaaaatcttc
    88801 ttaccatcat tggagtaaga gctatttcag gaaccagtac tataacacct ttgtctttct
    88861 gtataacctt agcagcaaga ttcagataca cttctgtttt tccgcttccg gttataccat
    88921 gaataagaaa cctgtcatat atctctttat caattgattg ttttattgta tcagtaacat
    88981 tcttttgttc aactgtaggt attttattac tatcaagcgc ttcatcagtg atttcaacat
    89041 gaatatctgt ttttttcggt ttatcaaata actctatata tccttttttc tccagtgttg
    89101 ataatatgtt tcttgaaata ttttcatagc tagcaaaatc attaacagtg atatcctcat
    89161 aactgcttaa tagttctaaa gctcttatat gcttttcatt ttttatctga tctttgaaaa
    89221 taagctctga tacaaaagaa ggttcgattt taagtcttgc tgccttttca aacttcttct
    89281 tgatatttct gataccctgt tccacaatct ttatcttatt agatttcatc tgttcattaa
    89341 gctgtttaat ccctccgctt agtgatttca gatatgtctg aaaaagagta agagtgccat
    89401 cctcctgcaa gacaagtttt tctacagata tcttaagacc taaaggaacc atcgcattaa
    89461 gtacctgacc atatgtgcag aaatactcat ctctaatcgt ttttgctaga atgaacatat
    89521 ttttagacaa tatagcttca tcatccagta tttcaataat atacttcatc ttaacatttt
    89581 taagtttgtc tggcataggt acgaaatcta aaaagtatgc tacttttgtg ctgtttcctt
    89641 ttccaaacgg aacaagaatt ctcattcctg tttcaagata aagcaacata tcatcaggaa
    89701 caagatagtt atatatcttg tcatatgtcc tggtggtatt atcaagtatt acagcagcta
    89761 ctgtcttatg ctccattttt taatatgcaa cagcccatac aaccatatgc ttggcttgtt
    89821 tgccacagca gacacattta tctgcaaatt cttcctggtc aaaaggaatg catctggaag
    89881 taactccggc ttcttccttt aggcgttttt cacattcttc atcaccacac cacatagctt
    89941 tcacaaatcc gcctttggtt ccaattattt ccttaagttc ctctatggtt tctgctttaa
    90001 aagtgttgtt gtcaagattc tgtttagctt caataaacat atttttctga atctcatcaa
    90061 gaagatcctt aactgtattt tctatatcag aaagacataa aggctgtttt tccaatgtat
    90121 ctcgtcttac cgacatgcac tgactgtttt ctatatctct aggacctatt tccagtctta
    90181 aaggtatacc cttcatttca tattcagcaa atttccagcc tggagactgg tcagatacat
    90241 ctatgtttgc tctaattccg gcttttttaa gtctttggag taatgcatct gctgcatcta
    90301 taactccttc tttatgctga gcaacaggta ttaccattac ctgtataggt gcaaccatag
    90361 ggggaagttt taatcctgaa tcatctccat gcgtcattat tatccctccg ataatcctgg
    90421 ttgacatacc ccaggaagtt tggaatggat tcttaagagt gttgtctttg tcagtaaatt
    90481 taatatcaaa cgctttagca aaaccgtcac caaagtagtg gcttgtcccg ctttgtaatg
    90541 cttttttgtc atgcatcatg cattcaattg tatatgtctc ttctgcacct gcgaatttct
    90601 ctttttctgt ctttcttcct ttaactacag gcattgcaag atagttctct gcaaattcag
    90661 catatattgc cagcatttgc tgagtttctt cccttgcttc tttaacagtg gaatgtatag
    90721 tatgtccttc ctgccacaag aattccctgt gtcttagaaa tggccttgta gccttttccc
    90781 agcggactac cgaacaccac tgattatatt ttttaggcag atcgcgccat gatgttatta
    90841 tatttttata atgatcacag aaaagtgttt ctgatgtagg tcttacacag attctttctg
    90901 tcagttcctc tgaaccgccg tgcgttaccc atgctacttc aggagcaaaa ccttcaacat
    90961 gatctttttc cttctgtaaa agagactcag gtataaacat tggcatatag acattctcat
    91021 gcccggtatt tttaaacatg ctatcaagaa tcttttgaat attttcccat aaagcataag
    91081 catatggtct taatattaca cagcctttaa cttgagaata gtctattagt tcagcttttc
    91141 tgactacatc tgtataccat ttagcgaaat cttcttctct tttggtaatc tgttctacat
    91201 ctttcttttc ttttgccata aattatatct cctgtttttt ctgtttttct tttattaaat
    91261 gtatttcttt gatgaaagta tccatatcat caaagtcttt gtatactgaa gcaaatctta
    91321 catatgcaac atagtctata tcaataagat gttccagcac tttatcacct atttctcttg
    91381 atgtcacttc tctaagatgc tgtttgtgaa agtccagctc tattgcatta accacatttt
    91441 ccagcatagt tatcggtacc ggtctttttg cacatgctat cataagcctg tttagcaatt
    91501 tggctttgtc aaaattctgt ctggactggt ctcttttaac tactacaata ggttttctct
    91561 ctatattctc atatgtagta aatctttctt tgcagtttag gcattcgcgc cttcttctaa
    91621 tggcatatcc ttcttctgaa ggtcttgaat cgattacctt atcttcttca tgtccgcaat
    91681 ttgggcattt catgtcttcc ggctcctttc tttataatac aaacaaaaac atggtctgtt
    91741 catagaatat gtaaaactca tatattcttg ttaaattata ttccataacg aatttaatcg
    91801 caatgtgccg tttttcacat aaaaattatg ataatcaatc tattttctgc agtttagcga
    91861 attccagtac aagcctctta actccggcta tatcaaaatc cacttcaact atatccatac
    91921 ctccatcatc atacaaatcc ttaattgaac cttcaccata ttttttatgt atcactctgt
    91981 caccaatgtt atagtctgct ttttccatat ggttaactgt ttttgttgta agtccaggta
    92041 tatccacagg ctttccaccc ttggttattg aaactgtatt gtcagaagaa aacatcttgc
    92101 taaatgagaa aatattacta tcatcagatt tatgcttctt cttccctcct tcaattagct
    92161 catatggtat ctcttttaaa aaccttgact ggtcacatgg ctgggtttta ccaaatttag
    92221 tcctttcctc agcatatatg cagtagagtt tctccatggc tctggttata cccacataac
    92281 ataatcttct ctcttcttcc agaccttcta aactttcagt tactgacata ctggagggaa
    92341 acaaaccctc ttccattccg acaagaaata ctactttgaa ctcaagtcct ttagctgaat
    92401 gaagtgtcat aatagttata tggtcatcag aatcatcttc tttatcaaca tctgtagaaa
    92461 gtgttacatt ttctaaaaat actgataagc taagttcctg tcctgactcc tcttccgaag
    92521 actgctgcat agtggttata gcgcttttta attcctttat gttctcaata cgttctcttc
    92581 catatatgga atcttcacgt atatagtgtt cgtcaatctc tgtaagtttt agaatcatat
    92641 catacaactg atcaagtttt cctggtttat catattgctt aagttcttca ataatatcag
    92701 caaatgcttc aagcttcgca ctagctgaag acaactgttc ataatccttt gcatttttaa
    92761 taatttcata tgatgaaagc tgagtctgtg aggagatata ctggattctt tttatagtag
    92821 tatcgccgat tcctcttttg ggttgattaa ttatacgaaa taaagaagcg tcatcatcgg
    92881 tgctgaaaac tactctcata tatgccagta catcctttac ttcttgtctg gaaaagaagc
    92941 ttagcccgcc gaatatcctg taatttagat ctctttctct gaatactctt tcaaacacac
    93001 gtgactgagc attagtacga tacagtactg caaaatcttt atatgagtaa ccttgattaa
    93061 tcatcttttt tatttcgctg gcaacatata tagcttcgtt catctcatta tatgctttgt
    93121 agacatttat tttgcatcct tgaggattac ttgttttaag tgtcttattt tttcttgaca
    93181 cattgttttt tataacatga tttgcagctt ttagtatatt ttcacatgag cgatagtttg
    93241 tttcaagctt gataacagta ctatcaggaa aatccttttc gaaattcaat atattcttta
    93301 tatttgctcc tctgaaactg tatatggact gatcatcatc accaacaaca aaaagattct
    93361 tatgcttctg agaaagcatt gcaaatattc tgtattgagc aatgttagtg tcctgaaact
    93421 catcaattaa tatgtatttg aagatgtttt ggaaatattc aagaacatac tgattattct
    93481 tcaaaagata atatgtattg aatattatgt catcaaaatc cattgcattg tattctttca
    93541 tcttttcctg atacagctta tatatatttc ccactgcttt tttctgaaaa tcatgagaat
    93601 gagccgcctg ataaacatca ggtcccatta aatcatcttt agctctgcct atttcagctg
    93661 ctacatagtt aggcgtgaat gccttatcag agatattcaa ttgcttcata cagtttttaa
    93721 ttacactttt cttatcatct gtatcatata tgagaaaatt gcttttaaaa cctgcttcat
    93781 ttggatattg tctgagcatt ttcaaacagg tggaatgaat tgtcccaagc cagaaaaatt
    93841 ttccttctaa tccttcttcg cataacttat ataccctttg ctttatctga cttgcggctt
    93901 tattagtaaa agtaatagca agtatctgat taggccttgc aagacccttt tgaataaggt
    93961 acgcgattct tcttgttaaa acacgtgttt ttcctgagcc tgcacctgcc agaaccagta
    94021 cggctccttg tgtattggta acggctctaa gctgctcttc atttaagttt tcaagtatac
    94081 tattgttttg tgacatattt acctcttatt tatcgctccc attattctac cattaatacg
    94141 ggtacattaa aaggaacaaa cctgattata ttgaactgct ttttatatta tgttactatt
    94201 caaatataga tttcaaacaa agaggtgtat atgacttctc agttaacttc cagagaaagg
    94261 ttcgtaagaa cacttacagg acaggatgta gacagggttc catttatgag aatattcggg
    94321 tcagataact atgcattatc atcatggtta aaatacagac ccaatctatc gctgtatatg
    94381 gataagctaa taggttttga aggtacatac agaggctggg caacaatacc tgtcaattat
    94441 ggcctgtgcg gacttcctcc agatatctgt cttgatgata atggtccgat acatatatac
    94501 aaaaacggta taggtgaaat aagaacatgg agcaaagata gggattatca cagcggagta
    94561 ttggaatatg ctctgaaaga tgcaaatgac tgggatagaa taaggccata tgttgtatgt
    94621 gatattgaaa aaagaatacc ttcagatatt gatgaacttt ctgagtttta cagacatcgt
    94681 gattatccct tacagcttac ctgcggagga gtgtacgggt tcatacgcaa gcttgcaggt
    94741 gacgagaatt taggatatct tttttttgat gatcctgatc ttgttaagga aataatatct
    94801 gaatatattg atatgctttt aaatctttgg gagagacttt gtgagaagat tgagtttgat
    94861 cttatagaaa gctgggaaga tatggcatat aaatgcggat ctattatctc tcctgagcat
    94921 ttcaatatgt ttcttgctcc acattataga aaaattcgga aatttgcaga tgctcatcat
    94981 atacccattc ttttagtcga cagtgacggt aacaccgacg tgcttgcaca gtggatgtat
    95041 gattcagggg taaattcaat atatcccttt gaatctcttg caggctgtaa tccaaaaaat
    95101 ctgcgtgcaa agcttccgaa tatgggagga attggtggac ttaataagga cagcatggct
    95161 catggcaaga aagagatgga tgaagaattg gaaaaagcaa tagaacttat taagttaaaa
    95221 agattcattc caggacctga ccacatggta ttaagcaatg taagttttga aaactacaga
    95281 tactttatga ccaggcttaa agatatagtt atgaatacaa aaccttagct gagaatgtaa
    95341 aaatggaaaa acagctaaca ggtaaagaat caatttgagc aaaaaataaa gataaaatat
    95401 atagatacaa aaaaaccggc cctaaagccg gttttggttt attatataaa aacttattcg
    95461 tagggtacca aaagaccgta atctccatcc ttccttttgt atactacatt cattacatta
    95521 gtttcttcat ttacaaacat gaagaactca tgattaagca tttccatctg cataactgcc
    95581 tcgtcaacag acataggttt gactggaaat ttcttggttt ttactacgtt atagtctgct
    95641 tctgaaaatt ctgtttcctg gcttataccc ttccaattct taggtacatc cccatgaaat
    95701 cttttttcaa gtcttgtctt attctttcta acctgtcttt caataatatc tactgactta
    95761 tcaatctgtc ctaatagatc attacctgaa agctctgctc tgaagatcac tcccttccat
    95821 actatggtgg actcaacttt tgcgtgtttg ttctgtttta atgcttcaac aactacagtt
    95881 gcttccgtac ttgcatcaaa aaatttgtcc agtttagcaa atttcttctc tgtccagtct
    95941 ttgacttcct cggtgatatc gattcttcgt ccgacaattg aaattttcat ataactgtct
    96001 cctttcaaat attatggctt ctgatatata tattatacct tatataaaat gaagtcacaa
    96061 tgaacctttt gttaacattt tatgaatctt atccgtagaa tacttccatg aggcacagtc
    96121 cgctggcctt caatgtgggt ccgcattttg atcggtcttt acttgaaatg atttcaggta
    96181 tctgttcagg ttcaatagct cccttaccga cttcagctaa cgttcctgca ataatcctta
    96241 ccatgttata taagaatcca ttgccttgta tatcaaaata tattaaaggt ccgtcctgag
    96301 aaatatctaa tgtgtatatt gtccttatag tcgtttttat actgcttcct gctgacataa
    96361 atgctctaaa atcatgttct cctataaaat atgaagcagc tttcagcatt ttatctacat
    96421 caagaggctg tttaacatgg catgtcgtat atttataaat tgctgatgga actgatctgt
    96481 ttagtattgt gaacctgtaa tgctttccag ttgcgctata acgggcatga aaatccatat
    96541 ctatttcaaa agattccagt atccttatat cttcaggcaa tacactgttt aaagccatac
    96601 agaagtttga tggtgggatg gtacagtcaa ttttaaagga agctgtctgg cccatagcat
    96661 taacttttgc atcagtccgg ccactgccat ttaatttaat tgtttgtccg gtaagttcaa
    96721 atactgattg ttctattttc tcttgaatag atattccatt aagctggcgt tgccatccac
    96781 tgtaattctt cccgctgtat tcaattatta gaagtatgtt ttttagcata tatatatttt
    96841 atgtcatggc atattcaaag taaacaaaaa gggcttttca gccctttttt agaataatca
    96901 tatttagcac cattacttat ttggtatttc ttccccaatt tcaatggtca acatcagtgt
    96961 attttcatct cttacaataa ctatgtcaac tatctgtccg acttcacatt cgtcaacaag
    97021 ttccttgaat tgagaagagc ttgaaatttc cgttccttta aatgatttaa tatagtccca
    97081 ttcctgcaat cctgccttat ctgcagcaga accttcttcg accataccta tatatacgcc
    97141 tatctcagtc agattcaatc catacatcat attgtacagt ttcaaatcat tttgggatgt
    97201 tatttctcct agtgatactc caagataagg tctccctttg acataaccgt aatttatcat
    97261 gtcatcaaga acattcttta tatcatttat aggtattgca aatcctattc cgtcaatctc
    97321 acttccatat gattttgcat tgattatacc tatcagttcc cctttggtat ttattaacgc
    97381 acctccagag ttaccaggat tcacagcggc atcagtctgt aatagaacca ttttttcacc
    97441 atcaagagtt atttctcttt ctaaaccact tatataaccg acagtcagac tctgagaaag
    97501 ataccctaat ggatttccta ttacaagaac taattctcct actacaattt catcactgtt
    97561 gcctataacc atatattcaa gatcatctgc atctatcttt aaaagagcaa ggtctaactg
    97621 tacatccagt cctataagtt ctgcttcgta actttcctta tcatttaaat atactgtaat
    97681 tttgttagca tttgctacta catgattatt agttaatatg tatccatctt tacttattat
    97741 tacgcctgaa cctgaacttg ttgaagtata tgttgaccat actgatgatg tttcaaattc
    97801 caccacaata ccgacaactg cattaacatt gtctgcatat atatcagcaa ctgaatcata
    97861 ttcctttatt gtactcgatg atgtgtatat tgatgaagaa tcattttcac ctgaaggctt
    97921 actagattgt gaatttaata catcttgatt ttcgtataat ttattcagct caactgcctt
    97981 ctggtaatat ctgcttgcca catatccgcc tgcaagacct acaagcaggg aaaaaacaga
    98041 gcatatagct attgctatta taaaggtttt cttgaaagtg gacatccttt tcttttcctg
    98101 cacatttgca tatgcggcat tattatattg ataatataga tttgtcggat tattgttatc
    98161 atttacgtta tatctgttat ccattaaaaa tacctcctta ttgtttattg cattataagt
    98221 atatttcttt cttgtgataa aaatgtgaaa gaacattata tgttatgtga tagaagtatt
    98281 tgcacagcct taacatatgt atcacagtgt tatcacattt tgagaataag ataaaattga
    98341 aattgttttt catagttggt acagacgtat attagatacg tttttgttcc gataccaaat
    98401 tttcagagga aggaggatat tactaaagaa aggcacatta gaaaaaatat cagccaagtc
    98461 aaaaattgtg cctaaaagcc gcaaatgcgg cttttttaat ttaaaggtgt ataaagattt
    98521 tactatattc attattgaaa aaagctaatt tgtgtatttt ttgttggaat ctcgttcata
    98581 taactgaata tcaaacttag attatgcatt acattgtggg attcacattc atttctaaat
    98641 atttccatca gatgatcatt attatcactt atgcattcat atgaataccc atatcggtta
    98701 atgtatttct gttttatacc agggaaatta tcatcaagag ccttatagaa atattcccgg
    98761 tcaccttctc tcattgttgt acccatattg aaattaatta ttccttttac atctgcatca
    98821 aaacaatatt ctaatacacc acggatatta ctttcattat catttatata tggcagtatc
    98881 ggtgtcatcc atactaccgt tggtatcttg ttttcctgta atacctttaa aacctcatat
    98941 cttttttttg tagtacatac atttggttca agtatcttac ataatttttc gtcatatgta
    99001 gttaaggtca tttgaactac acatttaact ttctcattta ttgatgccag aatgtctata
    99061 tctcttaata tcatatcgga ttttgtctgt atcgcaacac caaatccata atggtcaata
    99121 atctttagac attttcttgt aatcataaga tccttttcat aatgaagata aggatcacac
    99181 attgctcctg tacctatcat acatttattc cttttagtta taagggcttt ttctaaaagt
    99241 tgcggagcat tgattttcac ttctatatct tcaaaatcat gggtgaaatt atagcactta
    99301 ctcctactgt cacagtatat gcagccatga gtgcacccac ggtacatatt catgctgttg
    99361 tacgaagata atattccttt tgcttgcgta aagtgcataa tctttccttt attattaatc
    99421 tgagtgcttt tccagttcga accagaacac tacccctgca ttactgttat ataccccgta
    99481 attcccatta tgcatctcca taatagcctt aactatcgac aaacctaaac catatgattt
    99541 gctgttgcct cctcttgatt tatcttcctt atagaaactg ttaaacagat ttggtaatgc
    99601 ctgttcgctt atacctggat gagtgttata tatgctggtt cttatcttat tatctatttc
    99661 cttgatgtta atattaacta tctttttctc gtcacagtac tttatagcat tttccagaag
    99721 attagtaata acatgcgtta ttttattgta gtcgcctttt acaatgtaat catcctgata
    99781 tttgaaagta agcttgattt cagcttgctc aatcataatt cttatatctt tcaataccga
    99841 ttcaaccgct tcattcagac agaaaatgga atcgtcaatc tttgcagttc cgctctcaag
    99901 ctgggaaaga gtaagaagct gattaaccat caaattcatt ttcattgcct gttcagatat
    99961 tatcataagc tggcttttag tatcttcgtc atctttgtat ttttcaacta aaagctcaga
   100021 atatgcagtt ataagagaca gtggtgtttt aaagtcatga gatactgatg caataaattc
   100081 ttttttagtt ttatttgaac tttcctgctt ttccaaatca ttccttagca taattgctct
   100141 ctgttttaga taatctatac tgtcttgaag ctttctgctc atttcattta tactgtttcc
   100201 taaatcacct atctcatcat ttccagaaac ctcacatgtt tcagaaaaat ccagattaga
   100261 tatcttatta gcgaccttgt ttatgttcac tatgggtttt gttatcttgt cagaaataat
   100321 aataactgca ataactgcta ttaataaggt tatgatagag acgtacataa caaatctctc
   100381 tgcagtattt gcaactattg atatatattc ttttggtgag tttaatacaa tgtatgagcg
   100441 aactatattc ccattggtac tgtcagtgtt ttcatatacc atcgtaatta agtttatact
   100501 gggaaaacct cctacatttg tttcatcggt tttgtacccg tatacttttg taatatctgc
   100561 taattctcca tttgtgataa gaacacctat atagtcttca agtgctttaa tatcaggagt
   100621 gtcattagga agatactgcc cagttatctt ggttgaataa ccaatgtcaa cctgcattcc
   100681 ggtctcatcg taaacaaacc atataatttc tatattattt ctttcaatct tatcaaaagc
   100741 attgataagg ttgtctctga aatccggtgt ggaaatatca atttctgcga agtatccgtt
   100801 tatctcttcg ccatattcta ttaattcatt atgtttagct cttatataaa ggcgctcaaa
   100861 acccatatta ctgaaaaata tagtaacaag aacattgaaa agcagtacgc atataattat
   100921 tagtataagc ttattgcgca gtttaatagt tatccctcca gcttgtaccc atattttctt
   100981 actgtcttta tgcagtctcc tgattgtttc aactttgttc taaggcattt catatgtgtg
   101041 tctacggtcc ttgcatctcc aaaaaagtca tatccccata ctgcagacaa aagtttttct
   101101 ctggagtgaa cgatattctt gttctgtatg agatactgga gcaaatcaaa ctccttagga
   101161 gtaagattga tcttttcatc tttcacatat acttcatgtt cccttacttt taaaaggata
   101221 tcatttgcgc ttatatcatt gtcagcaaac atacctgatc ttttgagtac cgcttttacc
   101281 ctggacacaa gaacagcagg gctgaatggt tttgttatat aatcgtcagc tcccaatttc
   101341 aatccatgaa gctcatcata ctctgtattt cttgctgtaa gcataagaac aggtaccgtt
   101401 gattctatcc gaatagtctt tagcacttca aatccgtcaa gttctggcat cattatgtca
   101461 aggattacaa gggagacatt ttcactgtaa aataaatcta aagcttcctg tccgtctttt
   101521 gcctcaataa cagaatagcc ctcttttaca agacaagttt ttacgaggtt tcttaaggca
   101581 ggttcatctt ccgcaattag aatcttattc agcatttcta cctccaaaaa tacattcttt
   101641 tatatgatta taccacttat ttgtgataaa actgtgtttt ataaatcatg tgaatatgct
   101701 ctgattatag cattatatag atactgttat tttactgtat ctataacttc tccaataaca
   101761 ggcagttttg tgacatcata cttagctgaa aactcgttgt atatctgcat atatctttcc
   101821 tggtcagtct tttttacatc atgcagatat tcatgagtat caaaagctcc ttcagaaatc
   101881 tgtattgtaa gaacagcaag attagaacag tggtctgata ctctttcata attacttatc
   101941 agatcagaaa gaataaaacc tagttcaatt gtacagtttc ctccttgcag tctttcgatg
   102001 tggtgtgctt taagtataag cttcaattca tcaattatct gctctagcgg ttcaacctta
   102061 tatgcttttt caaaatcatt atttataaac gcatccattg aaagtacaag tatctcttca
   102121 acagcatcag ccattacttt taactcctgg tttgcctgtg gtgaaaaagt gatattctta
   102181 tcgtaaagct ctttagcaac ttcaagtata ttacatgcat gatcgcttat tctttcaaaa
   102241 tcacttatac tgtgaagtat ttttgatatt tccttactgt ctctgtcatt aagctctcgt
   102301 ccacttaatt taacaagata tgatccaagt ttatcttcgt ataaatctat catgtcttca
   102361 ttagctgtta ttatatttgc ttttttagga tcatagttat taattaaagg taaagcaatt
   102421 gatattgttt ctttagttat attagccata ttaagtatgt gctttttgca ctgatcaact
   102481 gctatagaag gagtatttaa aaagcgttcg tcaagaatga ttttctctgg tgatacacct
   102541 ttgtctttaa ctgtcttttc agcaaacttc acaagaagtt ttgaaaatgg aagaagaact
   102601 aatgttgata ataagttgaa taatgtatgt acaacagcta ttcctactgt attcaaagaa
   102661 gagtccataa aagtaaactt caatatagca ttcagaccat agaataaccc aatgaataaa
   102721 aaggtaccta taaggttgaa atacagatga atcatagcag ctctttttgc atttttgtta
   102781 gtaccgatac aagatatgag cgctgttgcg caagtgccga tattctgtcc cagtattatt
   102841 ggtattgcgc taccatatgt tattgctcct gttgtagata atgcctgtaa aacaccgacg
   102901 gaagccgatg aactttgaat tactgcagta aatattgcac cagtcagaat acctaacaaa
   102961 ggattagaaa acagtgtaag aatgcttgta aaaccttcaa cctcggctaa tggttttaca
   103021 gcacttgaca tagtggacat accaaacatt aatacgaaga atccaataag aacccttcct
   103081 aaatccttgt tcttatttct ttttccagcc attaacaata taacgcctgc aattcctagt
   103141 agtggagcaa atgtgtcagg cttaaataac tttattataa aagcatcgct gtctaggctt
   103201 acaagactta gaatccatga tgttatcgtc gtccctatat ttgcacccat aataacgcca
   103261 acagcttgag aaagcttcat caaacctgag ttaacaaatc caaccagcat taccgtagtt
   103321 gcagatgatg actgaacaag tgctgttact gcagctccta tcaatactcc tttcaaagga
   103381 ctattggttg ctttttcaat gactgattcc agtcttcttc ctgctaatct ttcaagacca
   103441 gaacccataa cactcattcc ataaaggaaa agtgttagtc cggcgaatat agttataact
   103501 gagaaaaagt tcatgtacac ctcaaaatca aattatatgt attatacaat attttgatat
   103561 gagtataaac acctgattag atattagtta gtacaattta taatatttaa agccataaat
   103621 acgaattttc ttactagtat atcggtattt tcttaatatg tgctgttttt aataattact
   103681 ggtttattta tctggttaat ttacctaatt agatatatta atgaaaatga atcaatattt
   103741 ttagatatta aaaaaacacc atataggtgt taatttaaaa ggtatgtccg gcagcgccct
   103801 attttcccgg gccgtcacca gccaagtatc t
//
LOCUS       DAOSXM010000002        99106 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2111
            NODE_110_length_99106_cov_12.080968, whole genome shotgun sequence.
ACCESSION   DAOSXM010000002 DAOSXM010000000
VERSION     DAOSXM010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864178
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 99106)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 99106)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:24:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,261
            CDSs (total)                      :: 2,229
            Genes (coding)                    :: 2,218
            CDSs (with protein)               :: 2,218
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 3 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..99106
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_110_length_99106_cov_12.080968"
                     /isolate="AMR_MDS_2111"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(126..923)
                     /locus_tag="PLQ68_00490"
     CDS             complement(126..923)
                     /locus_tag="PLQ68_00490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK71158.1"
                     /translation="MKKRTLISLSLLVVVILSLLLTTSCTPYGDRFSPSINNANALED
                     RYVIKELEYDIATYGGGPIKKPMVSQFNNISRQKTLNARLNAFNEMVGRDPVEDRNEQ
                     LYQSYEIRQMNYYLFSCEYFTAFETTVSNKGVNMIIGSEIDGTLIMNFPALLGNNIHS
                     DGWQEFFIFFNEAREEAGEKEITKEYMTTDNVSFVFEGDDLTDLTLRVLYKTADDTEQ
                     QEVPMKLNDIKGCLSERMQVYFSFTNNKDITYGEYALNIEEYKGYYE"
     gene            complement(1204..1280)
                     /locus_tag="PLQ68_00495"
     tRNA            complement(1204..1280)
                     /locus_tag="PLQ68_00495"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1244..1246),aa:Arg,seq:acg)
     gene            1499..2713
                     /locus_tag="PLQ68_00500"
     CDS             1499..2713
                     /locus_tag="PLQ68_00500"
                     /inference="COORDINATES: protein motif:HMM:NF018914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HXK71159.1"
                     /translation="MKEKFDSKSILYSDTLIPDIFISEYLPVLSCEAVKVYTFCCFLD
                     KTERSLDIFKIVRRLDIEENVLVSVLDELTKKHLLTLSGRDIFLNDIKGIEIDRLYKE
                     RTSIKPEDLGEKESVISAINNQFFDGNMPIYMYGCIEQWFKKYRFEDPVMMMLFSISN
                     EKGALTQNYIEAVAKDWFENGVKTVFDLEALFTEREKMRDIQSKIQKALNRKNPFTQY
                     ETDIINKWFNEYHYSFEIIEEALKKTVKISNPNIAYVDKILSSWYENEFKNIDDIESE
                     KAIKDLTPNELRMIVQEHYQKINMRNSMLFENRKVEVFKKAPAIETLYNDINDLLFKQ
                     AFSPDKKAIAEEIKNKNYEMTLLFKHHNIPDDYLTRQYDCELCKDTGVNNGRDCSCKM
                     EFLKSLSGKKEK"
     gene            complement(2718..4646)
                     /locus_tag="PLQ68_00505"
     CDS             complement(2718..4646)
                     /locus_tag="PLQ68_00505"
                     /inference="COORDINATES: protein motif:HMM:NF024904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HXK71160.1"
                     /translation="MRIVTAHIYGYGKFINKTFNFNQGMNTVIGYNETGKSTLMSFVK
                     SMLYGHKKNEREGKDGSLPEVKKYKPWNTDKYGGYLIVQTDNGRILRIERDFNSKSLT
                     VYNENNEDITSEFSFSKENGMLGQDLLGMDLECFMNSAFVCQDKSILYSEDKENIAQK
                     LMNISESAEEDVSVADSIKILKDGKTFLGNERTSKRRYNNLISNIRIEKAELVKMQIE
                     NQNCIEYLSEASLLKDEIKQLEHEEKLCVKKQVYQDTIKDIKNYEKNKCEADSLKKGL
                     SVIDIEEYEKSKELLDEEIKAVNFNEEDKDNLYKNIKINNFIITLLIVIGVLILTAGI
                     ILSIIVDMWFLLLALIAAGCGIGLFLLSKKNRENHSIIERIDEINHKKVNLEKTKAKL
                     EVNKSKEDLIRTYEKFMSDILINQDVADFEALKEKAEVFSPKVAYPQISHDECVRQIQ
                     RKRERLAVINAQIEKYLKSDDQIASQQEKIAVLEEQTIHLENKQKAIDYAILGIEEAS
                     RQIKEEIIPKMNEKMSSYLYRITAKKHDNLLTGSQMDLNTEYSDRIRSANNFSEGTLR
                     QMYLAFRLAASNVFSNKKVAPVFMDEALVYYDKERKKSTFDFLYEMSTKTQIIYFATD
                     IEDALIKNKEINIINLTD"
     gene            complement(4643..5686)
                     /locus_tag="PLQ68_00510"
     CDS             complement(4643..5686)
                     /locus_tag="PLQ68_00510"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HXK71161.1"
                     /translation="MISFYHLADIHLDQPFSTLASVDGFPSKRREEIFSEFKRILKQA
                     IEEQPDFLFISGDMYEHEYTKIKTIKEINEYFSKLKGRVILICGNHDPESKNSFYSSY
                     PWASNVFILGRKNPYMFFEEKKVCVYGIGYDTGGGQFRELETIKTDRRYTNVLMLHGD
                     ADLSISTYNSVNSELLKDIGFDYVAMGHNHKMYVKDNIYNPGSLCQLKVDDEGEHGYF
                     KGDLETKTIEFVESRSRKYADITIPYEKLSTFEQEYPDKSTIYRIKVTGMKNENDIVN
                     PIEGYDYLHIIDKRELPKRANCETVSQGIKGRFEKVMNERIMKASTEEKEIYKDALDL
                     GLKALMNEGLDLE"
     gene            complement(5691..6071)
                     /gene="acpS"
                     /locus_tag="PLQ68_00515"
     CDS             complement(5691..6071)
                     /gene="acpS"
                     /locus_tag="PLQ68_00515"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006807953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HXK71162.1"
                     /translation="MRISCGTDIVENKRILDAVNRSEKFLLKNFTPKEISYCESKKAV
                     MAQSLAARFAGKEAVAKALGTGFIGFVQPVQIEILNDEKGKPYVVLGEDLKKDFPDIT
                     EISISLSHCNDYSVAYAVITFKEE"
     gene            complement(6068..6922)
                     /gene="ispE"
                     /locus_tag="PLQ68_00520"
     CDS             complement(6068..6922)
                     /gene="ispE"
                     /locus_tag="PLQ68_00520"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HXK71163.1"
                     /translation="MIVQKAYAKINIGLDIKGLREDRYHEIDTIMQTVELGDRITFTE
                     KEKGIQFWCDTPSIKQNENTAFIAANLFMKEMDIKEGIKIDLYKTIPISAGLGGGSAD
                     AAAVLRGLNYIFKTNLSFEQLEEMSVKIGSDVPFCIRGGTQRVNGKGEVLTCLKDFSD
                     VWFVIIKPKESVDTSWAYREYDKCKNPDRPDMDLIQETMDKRDLVGLKGKIVNIFQNV
                     VIPHKPSIQEALDDLNKTEPISAFMTGSGSATVAVYKEMKQASMAYAELAKKYIDIYI
                     TKTSGGLS"
     gene            complement(6925..7956)
                     /gene="tdh"
                     /locus_tag="PLQ68_00525"
     CDS             complement(6925..7956)
                     /gene="tdh"
                     /locus_tag="PLQ68_00525"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF003808.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HXK71164.1"
                     /translation="MKAIIKAEAGMGNVQLVQRPVPEINENEVLIKVKKAGICGTDFH
                     VYKWDKWSQNRIKPPLIMGHEFAGEIADMGKAVKGFHIGQRVSAEGHITCQKCKLCRT
                     GRAHICNDIEIIGVDRDGCFAEYIKMPALNVWPLDDEIEDKYGAIMDPLGNAMHTVMA
                     QPISSRDVLITGGGSIGLFATAIARRFGAARLVVSEPQPYKAELAMKAGADVVINPLG
                     DDPKGQILEAFNGHQPEVVLEMSGFPSAFQLGLEVIENGGDMAILGIPSEDLNIDMGK
                     YVIFKGITLRGINGRRMFDTWYQCQNFLKSNASFIDHVITHELPMEDVNEGFKMMADS
                     KAVKVVLKI"
     gene            8085..8591
                     /locus_tag="PLQ68_00530"
     CDS             8085..8591
                     /locus_tag="PLQ68_00530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK71165.1"
                     /translation="MKKILTLIIAIITISILAGCSTYNYINSYPVNIDDLTVEYIRTN
                     GYYEDIEYPLVFVIKDKDQLESYFNEKKDKYNMESDGEDSFSSKMYKYDSSFFESNIL
                     IALLIEEPSGSIRHELTLVEEEDNKITFGITRDVPEIGTTDMAQWHILISMPSHLYNN
                     QDIDVEFN"
     gene            complement(8598..9737)
                     /locus_tag="PLQ68_00535"
     CDS             complement(8598..9737)
                     /locus_tag="PLQ68_00535"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HXK71166.1"
                     /translation="MDRAAFASTLYIELNLFAVSIMLVLLIFIRSKKSRLLLDQKLFI
                     TMVISNIFLLVLDSAMVLLDGVPGQYARIFMYISTFLYYIFNTLICLFWYLYVHYFIY
                     RNKVALKKQIFTIGVPFFAVAIMAIISLFADVIYYIDADNIYHRGRYFLVMVLICFLY
                     IAYAYILLIVKRKMINKREFLSLALFIVPAVIGGVIQILVYGITLLWICATLSLLIIY
                     INIQNGQLYKDYLTDLYNRRQFDNYIRAKINSSNKKIAGLMIDIDSFKKINDLYGHDN
                     GDLALKYTAQILRETFSEKDFIARYGGDEFVVVIEVSDEEEPYEYIDKINYGFEKFNS
                     TKELPFKITASVGYGIHVSNETVTDFFRKMDKMMYINKNKSKETA"
     gene            9899..10333
                     /locus_tag="PLQ68_00540"
     CDS             9899..10333
                     /locus_tag="PLQ68_00540"
                     /inference="COORDINATES: protein motif:HMM:NF016060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HXK71167.1"
                     /translation="MKDLFEKLMQRRLFRELFLLFKYGLVGIINTCITTGIFLLLSYL
                     GLEYVLYTIIGYAVGICISFLLNRKYTFKKENENATRQIILFFTISVSLLAIVQLIQY
                     LLIDIYNLQEWLGVGIGMVFYTGTGFILNRNIVFKYTDKPEE"
     gene            10337..10924
                     /locus_tag="PLQ68_00545"
     CDS             10337..10924
                     /locus_tag="PLQ68_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015312334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HXK71168.1"
                     /translation="MTDRIIKQIEFIKEIDKMKNILRMTLVSESERRENDAEHSWHLA
                     MMAMLLEEYSDKPVDISHVLKMTLVHDLVEIYAGDTFAYDMEGNKDKEERENRAAEKL
                     FSMLPDDQKDLIMSLWKEFDAMETDDSLFANAMDRFQPFLSNVMTSGHTWKIGKVTKE
                     KILSRMDPIRIATPALWPYILDTVEQLHQKGYIYK"
     gene            complement(10925..13348)
                     /locus_tag="PLQ68_00550"
     CDS             complement(10925..13348)
                     /locus_tag="PLQ68_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007048971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HXK71169.1"
                     /translation="MAEKKFYITGMTCSVCVNHVETAVKELKGVKQAGVNLSNGTLKV
                     VYDETSVTEADIEKAVKKEGYGIKKGDEYIEEEKKMRIRIISSVVILVILMYLSMGPM
                     INIPIPKIFTTDIVLFFSILLVLTAIIAILNRSYFIKGFQQIIKLRPNMDSLVAAGAG
                     AAIIYGLYIFVRVIIEKNPNAHYVHDIYFESAAMILTLVTLGKYFETKKKKKTGEALR
                     KLMDLTPKVSVILKDDQEIEILSEDIQLDDLVIIRQGDSIPCDGTVVKGQGYVDQSSV
                     TGEPIPEEKLEGDKVISGCICKSGYFIFKAEQVGENTTVAKIVRLVEDSSMKKAPIAK
                     LADEISRWFVPVVTLIALSSFVIWLLAGKGFDFAFSIAISVLVISCPCALGLATPISV
                     IVGTGKAAEHNILIKTGEALEILHKVDTVVLDKTGTITEGKPTVVAVHAVIGEENHLL
                     SMAASLEKMSEHPLADAILTKNKELGLSLEEVKDFQPLQGEGVLGSIGPNIYKVGNRR
                     LMENSNINVQSVSEIYNDYADKGYTPVYIARNDEIMGIIAIADVLKPESIKAIKAIKK
                     LGINVIMLTGDNERSARAMADIAGVDDVIAGVLPVQKEQKVSALREKGKIVAMVGDGI
                     NDAPALIASDVGIAIGAGTDIAIESADVILMKSSLMDVVTSIKLSKKVFTNIKQNLFW
                     AFLYNTISIPIAAGVFYNVFGWTLSPMIAAAAMSMSSISVVANALRLKRFKPNNEKGA
                     KMKKEITIEGMSCSHCSKRVEDALNRLENTHAKIDLKKKTAFVETEQSDEAITKAVKE
                     AGYLVKKIK"
     gene            complement(13371..14693)
                     /locus_tag="PLQ68_00555"
     CDS             complement(13371..14693)
                     /locus_tag="PLQ68_00555"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HXK71170.1"
                     /translation="MQLKSKLSIFLLIIIIIASMFAYMGAILTSNRSFIDNIKENQRY
                     VAETIIAANEETDLPLERIIAITYNALYPVVISKDIEDLNLNDKDKNILEEGGIVFIK
                     QKVIPVGISVIKTNEFYLRVGYQPENNLFRNYAMLLAYALLFSIIVFMLIIMGISNKA
                     MKPIIQLNNATQEVAKGNFDVAVFNDSQDEIGQLTESFNKMAKELKSIEYLRKDFVSN
                     VSHEFKTPISSILGFAKLLENENLTDQEKKEYLEIIVEESERLSNLSTNIMKLSKLES
                     QDIVFNKAEYSLDEQIRRVILLLEPKWCEKNIDFDIDMENISFLADEDLMQQVWINLL
                     NNAIVYSYQNGTIHIRMYKYKEEEKAVIEIQDEGMGMDKHTKDRMFEKFFKGDSSHSK
                     EGAGLGLALVKRIVDLHQGVIEVESELGKGTTIKITFPNEFRGWESIT"
     gene            complement(14677..15363)
                     /locus_tag="PLQ68_00560"
     CDS             complement(14677..15363)
                     /locus_tag="PLQ68_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008513972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HXK71171.1"
                     /translation="MFNILIVEDDKNLRKLIGTVLKQNGFNVFSANDGEQALEILEST
                     KADLIICDIMMPNMDGYELTSSLRDAGYNMPILMITAKDTMEDKRKGFISGTDDYMVK
                     PINIDEMLLRINALLRRSKISTEHRLEAGDVVLDYDTLSVQSSKETIILPKKEFMLLF
                     KLMSYPKKIFTRQQLMDEIWGLDTETDERTVDVHIKRLREKLSDIKEFEILTVRGLGY
                     KAEKKNATEV"
     gene            complement(15424..16101)
                     /locus_tag="PLQ68_00565"
     CDS             complement(15424..16101)
                     /locus_tag="PLQ68_00565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HXK71172.1"
                     /translation="MGNMFYMIVITKREYEEAYHEFFKENEIFMVLSEFCKGTAQKKT
                     LDYLGIEKTEKIMFQAIADCKHRNEIMKGLINQMEIEVPGNGIAFTIPLESIGGESCL
                     KYLAKGQNIKGEGEKMDDIKYSLIIVIANRGSSDLVMEAARSANARGGTVVQAKGTGE
                     DIKERFFGVSIASEKEMIYILCKKENRENIMGTIMERAGTDTEAHAVAFSIPVDQIAG
                     LRSVTVE"
     gene            complement(16113..17657)
                     /locus_tag="PLQ68_00570"
     CDS             complement(16113..17657)
                     /locus_tag="PLQ68_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006444646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HXK71173.1"
                     /translation="MSKENREFIKHNRTILLEKLKESLSSVLPVTIIVFILSFIFFPI
                     KSGMMLTFIIGAVMLILGMGLFTLGADIAMMPIGEHVGSAMTRSRKIWLILLVAFIVG
                     TAITISEPDLTVLAEQVPGIPGMVLVVTVAVGVGLFLLLAMLRIIFKIRLSYLLVGLY
                     IAVFILAFFIPKEFLAVAFDSGGVTTGPMTVPFILALGVGVSSIRSDKDAQSDSFGLV
                     GLCSVGPILAVLILGVIYKGIASNYEYKPLPDAADTQKITMLFGSDFPHRIMEVAIAL
                     IPIIVFFGLFQIIVLKLKKGQIIRIVFGLIYTYIGLVLFLTGVNVGFMPLGSYLGQII
                     GSLANNWIIVPIGMVIGYFIVIAEPAVHVLNKQVVEMTSGAIPKKAITTSLSIGVSVS
                     VGLSMVRILTGLPLMYLLVPGYAISLALTFFVPKVFTAIAFDSGGVASGPMTATFLLP
                     LAIGVCLGVGGNVVVDAFGLVAMVAMTPLITIQALGVYYRVKVKSTEKQEKARARKRE
                     KIINFE"
     gene            17893..18357
                     /locus_tag="PLQ68_00575"
     CDS             17893..18357
                     /locus_tag="PLQ68_00575"
                     /inference="COORDINATES: protein motif:HMM:NF014012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit E"
                     /protein_id="HXK71174.1"
                     /translation="MLKFTTLFTLVWIALNEELSIFNVLAGLILSVITILFTGSLFFN
                     KGYISTYQIPFYRMIVYHLYVVFLIFKSGMSAIPRIIAGNDKTKTIKYLSGCKDDFAL
                     SLLSNAITLTPGTVTLDLTKNELTVLCFTDSKNNALFDIENARKIEKILEGR"
     gene            18359..18622
                     /locus_tag="PLQ68_00580"
     CDS             18359..18622
                     /locus_tag="PLQ68_00580"
                     /inference="COORDINATES: protein motif:HMM:NF015993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015075 - monoatomic ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H+ antiporter complex subunit
                     F"
                     /protein_id="HXK71175.1"
                     /translation="MAFYNIALILLAIMFILVIIRSVIGPTIWDRLLGLSMLSAKIIT
                     FICLFALVSGKTMFLDVALIFSLLGFISNVLISRYVRRKSNSD"
     gene            18615..18920
                     /gene="mnhG"
                     /locus_tag="PLQ68_00585"
     CDS             18615..18920
                     /gene="mnhG"
                     /locus_tag="PLQ68_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013781494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H(+) antiporter subunit G"
                     /protein_id="HXK71176.1"
                     /translation="MIRTIISSIIISAGLFFMTFGIIGVYRFSNFYARILITAKVETV
                     GFLAVMIGILVQSGFSYFSFKIVLITLFFMLTNPLSTHAIGRSAFYSNLSEKEMDND"
     gene            18913..19464
                     /locus_tag="PLQ68_00590"
     CDS             18913..19464
                     /locus_tag="PLQ68_00590"
                     /inference="COORDINATES: protein motif:HMM:NF024641.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4040 domain-containing protein"
                     /protein_id="HXK71177.1"
                     /translation="MIEKIILLLLLALAIITIICKQYKRTVIFLGIFSLLSALLYVIY
                     SAPDVAIAEAVIGCGLSTVFYLVAIKKAEIFKVCIISENQSSNESTDLNNLLGGLKKD
                     IENRNLLCEINSTVKSVKEAMASESDLIIHEQVDRITIYGLENDHIFEGLCDDIDKNC
                     VINKKILITKVSDVKRNKETVNE"
     gene            19457..19720
                     /locus_tag="PLQ68_00595"
     CDS             19457..19720
                     /locus_tag="PLQ68_00595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK71178.1"
                     /translation="MSKFKTIAVIILMLALIITIFILSDFSESNTSTADYYIENSEKD
                     TAAINAVTAIYLNYRVYDSIFETLILLVSVSAVIHFSWRKDHE"
     gene            19713..20108
                     /locus_tag="PLQ68_00600"
     CDS             19713..20108
                     /locus_tag="PLQ68_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012062399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MnhB domain-containing protein"
                     /protein_id="HXK71179.1"
                     /translation="MSDKSYLMSHFIGFMYPIILLIGVYIIVNGHQSPGGGFHGGAVL
                     ATVFICRYLENPNEDLRIHSLEILEKLLFFLILLLPVVLLFNNISMSIISNKLYLLIM
                     NILIGLKVCTGLSIIFYRFVFYEGGGIWN"
     gene            20099..20452
                     /locus_tag="PLQ68_00605"
     CDS             20099..20452
                     /locus_tag="PLQ68_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013781490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter subunit C"
                     /protein_id="HXK71180.1"
                     /translation="MELITGENISILIFFAGFSGLMLKRNVIKSIICIGIMQAAIIIF
                     FLSSGLSPDNVPPIDATGLQNVADPLPQALMITDIVIGIGVTAMALSLFIHMYHQFGT
                     SDWEKAFKLRKDSEE"
     gene            20449..22071
                     /locus_tag="PLQ68_00610"
     CDS             20449..22071
                     /locus_tag="PLQ68_00610"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HXK71181.1"
                     /translation="MNNILVYLVLFPILTGSFLGLLKCNNTRKRKILTIVTIFIQSLL
                     LIGSLYVLTYVKDFGTTAQNIGNWPDFIGITLKADIYAVSFVLMSISLFTFAIIYQLT
                     ENDIESRVFSLLIILEGLIIGIFLSNDLFNIFVLIEVATIVISLLIMYKKDTISTYDG
                     MLYLLVNIAAMSLFLFGIGMLYKMTGVIDLTGLKDVLSVFPDKKALIVPYGLMLTAVC
                     LKSAVLPLFSWLPHAHATPSAPSAISAVLSGIYVKSGIYLFLRLQNVFSDVFDTYYLF
                     LILGFVTAVAGFMLAVSQKDIKLILAYHTISQIGMIMMGLSMNSESAYLGAVYHIINH
                     AFFKSCLFLSAGLIISAYKSRNVYKIKGVFKNMPITATACVMAILGITGAPLFNGSIS
                     KYWISYGAKGGWAEYALILINIGTMISFIKYSGMLTGNYDSTLVKISKKRSVPVLILS
                     VMCLLGGILSEPIISFLFNVQMSPNLLDYLEKSLLFFVSLGIAYLLYRYAISKVNLYK
                     IASRLELGFNGICISMGVFFLVMLLYIRIFAM"
     gene            22133..22864
                     /locus_tag="PLQ68_00615"
     CDS             22133..22864
                     /locus_tag="PLQ68_00615"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HXK71182.1"
                     /translation="MNKVIFWDFHNTLAYKHNFFIDCIIIVLDKYQKGHTVTHESLYP
                     HMQTGFPWLEPDKEYHHLKNPQKWWDNLYPVFINALTSNGIDKDKALLYTKQAHNIMT
                     GPDYYTLFDDTLETLKRAKINGYRNIILSNHIPELPQMAKQLGVMDYIDICISSANIG
                     YEKPNRKIYEYAVEAADNPDTVWMVGDNYEADYLGATSAGIKTVLVRNPLDEVNDVSK
                     TEKETKKVEYYSDDIKGALDIILQQ"
     gene            complement(22888..23580)
                     /locus_tag="PLQ68_00620"
     CDS             complement(22888..23580)
                     /locus_tag="PLQ68_00620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK71183.1"
                     /translation="MRSIASVIILFFALVVFVSLLIAAYYLIYKKLINKGLNDPNNTK
                     RNIPSLPSFILAAILTVIIIYGIIISVSAIISRNEENRVEPPICSYTEIDLDKEDNEK
                     KYSEIYSISENKGYDKYEEKINDIRFTYFISQSEYDTYHPSFIVFAEYTGKDKILHRG
                     VNGTFYYIDNSLIGGYGYAGGDGTNILCFIGDASYESIFELKVAFYNTHAKSEDFKEG
                     AAAVETLRFVVD"
     gene            complement(23591..24037)
                     /locus_tag="PLQ68_00625"
     CDS             complement(23591..24037)
                     /locus_tag="PLQ68_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002852382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HXK71184.1"
                     /translation="MKVKLLASKERYEEIEKELKALGIDIDDDSEFTFSEDNAYVNHL
                     NGKLEDILHRVNVEDVIYVESLAHDVLVHTSEAIYRIKERLWQLENLLDPDKFIRISN
                     SVIVSKKAIKSIKPALSQKFMLKLKNGNIVDVTRSYYYIFKDKLGI"
     gene            complement(24171..24980)
                     /locus_tag="PLQ68_00630"
     CDS             complement(24171..24980)
                     /locus_tag="PLQ68_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002580450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylase"
                     /protein_id="HXK71185.1"
                     /translation="MDYQKLILGMEKYGISTKDICINCIGVKEEHIHKNVVIAPWWEP
                     SLFPDFQTAEYISKSISDHIKVWNICNDDCEFTYIKTGIGASVLMDIVLSLGMTSCEK
                     IVFIGSVGALDSDICIGDIVIPEYSVCGDGASRYIDSDNLTKGDIFGKKEYPDESMFN
                     KSKEIAENLCKEHNVKWHIAKTFSIDTIFAQFYHLDEIIEIGCNVIEMETAAAFKAAK
                     LANIPITAVFSVSDNTVTRKSLITGRTKAERDYRRHTRRVLFPKIIKELFD"
     gene            25137..26747
                     /locus_tag="PLQ68_00635"
     CDS             25137..26747
                     /locus_tag="PLQ68_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004096628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HXK71186.1"
                     /translation="MTDISNTINNEVTRRRTFAIISHPDAGKTTLTEKLLLYGGAIRL
                     AGAVKGRKASKHAVSDWMEIEKQRGISVTSSVMFFDYNGYRINILDTPGHQDFSEDTY
                     RTLTAADCAVMLIDGAKGVEAQTRKLFAVCRLRHIPIFTFVNKLDRYCKSPFELMDEI
                     EKVLGIRAYPMNWPVGEDGSNLGVYNRKNKNIDLFEAKEHGQRALSSKLIPLSDPQTK
                     DILGARIYNKLCEDIELLDMAGDDFDFEKVKTGELTPMFFGSAMTNFGVKPFLKEFIE
                     LAPSPKNKNTESLNELPDNNNFSGFVFKIQANMDPAHRNRIAFFRICSGKFEKGMEVI
                     HARTGKTIRLAQPQQFFAQERTVAEEGFAGDIIGVVDNGLLNIGDALYTGKTPFVFDG
                     IPIFPAEHFAQVRTPDAMKRKQFLKGINQISEEGSIQIFKQPNIGVETLIVGVVGVLQ
                     FEVLEYRMKNEYGVDIVIEKLPYNLARWVMTKTVTREMLGFADSAKAVEDRLGRPVIL
                     YNNEWTLNRAQEKNKDVEFLEIPPIINQ"
     gene            complement(26763..27755)
                     /locus_tag="PLQ68_00640"
     CDS             complement(26763..27755)
                     /locus_tag="PLQ68_00640"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HXK71187.1"
                     /translation="MGKITILVNKAMQEKVFNEKYINRLGKQGDVFIYDKDDFVDSGY
                     VIDFVKESEIIITSWGTPSLSKEILDVCPDLKAVLHAAGSVKPIISDELLKRKIRVTS
                     SAIALGEGVAETALAFAISACKGFYNLGKDTAKGLWGENKAIVKDFYDITVGVISGGF
                     VGRHMVKLLKNFHVDILMYDPTLSKEYIENLGAKKVELEKLLCDSDVISIHAPSIPAT
                     DNMLNKDNLCLIKDGAVLINTARGTVVNEIDLIEELKKGRFFACIDVTNPEPPDVNNE
                     LRYMENVIMTPHIAGAVNNGCKRIALHICEEMERLNTGEKMKTEINLEDLSRMA"
     gene            complement(27817..28764)
                     /locus_tag="PLQ68_00645"
     CDS             complement(27817..28764)
                     /locus_tag="PLQ68_00645"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HXK71188.1"
                     /translation="MIKIGGVNIDVSHPKAFASNLESHNINMRYEYIFNDGFRSDEEV
                     NWFINRFNLKSRAGSIEEMADKVDIGFIQSCNWEKHIDQAMPFISKGKPVFIDKPIVG
                     SMKDIERIEKLVEEGAKILGSSSLRYSKEIKEFLSKPVEERGDIISIFGTSGVDEFNY
                     SVHIVEAMSALAGAKGISCQYIGKGTDGDNAICEMYSIEYENGIRGVYYSHIGKWQPF
                     HITVMTTKGTFSFVIDTNDLYRTLLEAVCDYMEGRNNVFSDIYTLTNCTKIMLCGKKS
                     RDELNGTAVYLDNLTDNDKFDGYEFEKFYSHSQGIVYKD"
     gene            29126..29668
                     /locus_tag="PLQ68_00650"
     CDS             29126..29668
                     /locus_tag="PLQ68_00650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HXK71189.1"
                     /translation="MIYYTADQHFGHSNIIYLCNRPFKDISIMDESLIMNWNNTVTSE
                     DIVYILGDLIFRSDKHAAYYIERLNGLKHLILGNHDHKWIKNCNLSKYFASVSDYLQI
                     KDGETHIALCHYPMLSWSGAARGSLHVHAHIHNIREGLVFETLRKMDNALNAGVEVNG
                     YKPVTLKELILNNAKFKISD"
     gene            complement(29677..30675)
                     /locus_tag="PLQ68_00655"
     CDS             complement(29677..30675)
                     /locus_tag="PLQ68_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010074265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HXK71190.1"
                     /translation="MELKTISQVSRMFNISTRTLRYYEQIGLIQSIRTDDYAYRIYDE
                     NTVIYLQQIIILRKLRIPLKQIVEILKRKDTAATIKVFQQNLIDIEDEITALSTIKNV
                     IQAFLERLQVHTDKLWLLNGDSLLRIVNSLTVSKINFKEEKSMEELNQANEKISKLND
                     IRIIYLPPMTVAAYSATGENCEGKAADMINRFVMENNLLKIKPDIRHFGFDCSAGKTG
                     VGESSYKYQMWVSIPEDMVVPKPLVKRIFDGGLYAAHMIKMGDFDHWILLRNWVMNND
                     KYEHDWHSIRCSPYEMDMERCFEEQLNYLNNLKNAEFKNEEMQLDLLFPIKEKKKE"
     gene            complement(30765..31334)
                     /locus_tag="PLQ68_00660"
     CDS             complement(30765..31334)
                     /locus_tag="PLQ68_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008977830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK71191.1"
                     /translation="MRKDVPELLFISRDEFRVWLNKNAEKSDGVFLVFSKTKEMVTLS
                     ANDALEEALCFGWIDGQMKSIDNTKYIKYFSKRRPKSNWSEKNKLTVGSLQKKGLMTE
                     LGEKAVETAKVNGIWDLPKSESITDEQIKTFTDKLKGISPAYENYNNMPQSVKTIYIR
                     RFLSFKTDEARERDFLKIIERLNRNLKPM"
     gene            complement(31376..32011)
                     /locus_tag="PLQ68_00665"
     CDS             complement(31376..32011)
                     /locus_tag="PLQ68_00665"
                     /inference="COORDINATES: protein motif:HMM:NF013078.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HXK71192.1"
                     /translation="MPLPMVHLGTARVYAKDYSELLHCPEYYLGCISPDAIHVRGNID
                     KKDKRITHLHAEGPEWENNVINFLKQNRNKKNYNFLLGYGIHILTDIIWWETVYIKFK
                     QEYIKDQNPVQDLKSAYYNDTDQLDFELYKNSEWRESIWSLLKKSESFFVEGLLEESE
                     ITAWKSHTLKYFDTKVSRHKNPIRYFTIEELEVFMKDAADRIKKILEKEGF"
     gene            complement(32052..32978)
                     /locus_tag="PLQ68_00670"
     CDS             complement(32052..32978)
                     /locus_tag="PLQ68_00670"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HXK71193.1"
                     /translation="MHDLDWERSKPILDIDVDEIGNIFSEYKNDILIIDFSPIQSGCR
                     NCNYVVNTSKDKYLLRISDKSSFNNEITAFEIVKGRINVSELLFYKTKGEYIYHIYQY
                     INGVSLQKHIVDNKRCDKSLLKQVAKSAAIIHSTPIEETKKLAELDIPPYEIWYQVFL
                     DIPTVRDRLGLNLCKRIHRLVTDKYELIAQIDSYKSFIHSDFRPLNMLVDEKNSIYYI
                     DWEAALWGHTLADIGQFFRYRTFFSNDDIRLFEKIYNTYADKKLPSNWYELSLFRDLV
                     NPLQLLSIKNEAPLRNADLISVIEGILAFWDY"
     gene            33295..33909
                     /locus_tag="PLQ68_00675"
     CDS             33295..33909
                     /locus_tag="PLQ68_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015515777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6088 family protein"
                     /protein_id="HXK71194.1"
                     /translation="MGNLSYNNLIYNRILSLPPESAFTATDFVDIADYKAVSKSLERL
                     EDEKKIRRVIRGVYDCPKYSKLLGEFEAPSPHHIAIAIARNNNWTIAPSGNTALNQLG
                     LSTQVTAYWLYISDGPYKTYDINNVKIEFKHRNNKEISGMSYKAAMVIQAIKTLKQQY
                     VSEGIIKHLKQSLTDEEKAKLLGEAKQTTTWVYSIIKKICKSGE"
     gene            33912..34910
                     /locus_tag="PLQ68_00680"
     CDS             33912..34910
                     /locus_tag="PLQ68_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015515776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HXK71195.1"
                     /translation="MYEVARTTINERRALFRNTSQKMNVHEAIIEKDFWVCLTLDYLF
                     QRCIYKSHLVFKGGTCLSKVYNLIQRFSEDIDLILDWRVLGYRMTEPWDVRSKTKQEQ
                     FNREANERTILFLRDTLLPQITEELSDIIGEKAEFSINNDDPQTIIFNYPHIFNAEAI
                     LQGIKLEIGALAAWNPVSMKTITPYAAEKYSRAFKKPNTEVLTVEPERTFWEKITILH
                     HEANRPVSSLMPQRYSRHYYDLYCIAKTEVKDRAISNIAMLTNVDEFKMRFYPRSWAR
                     YQDAKPGTLKLLPPAHNLRLLEEDYENMGEMIFGPRPGFKEMMDAITKLENEINAS"
     gene            35371..35760
                     /locus_tag="PLQ68_00685"
     CDS             35371..35760
                     /locus_tag="PLQ68_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015042146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK71196.1"
                     /translation="MYNIKTSTDLSLLHDKGILLSFGEICWNKINLISGAMTAPLVEA
                     IWAFTENDAESMERILVTLNHLYHNDHKVEMIAVLRILYDVSGLQFPEDVQLLAEHPK
                     TRQYFLFSFLLDMDDCMQEFMTEELKG"
     gene            36154..36936
                     /locus_tag="PLQ68_00690"
     CDS             36154..36936
                     /locus_tag="PLQ68_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009620086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HXK71197.1"
                     /translation="MHQISIFSWFGFPIPMEERFKLIKEVGFDGVLLWWSDEYAEVDG
                     DKLLHPDLARQKGLFIENIHTPIFRNNYLWEDGIDGDVLEKTLLECIEDCKRFEIPTA
                     VVHITHGINPHPYNQIGISRIKRLVEVAERNNINIALENLRRPEYLDFIFTHIHSDRL
                     GFCYDSGHEKCYTKGTDMLSKYGSSLMALHLHDNDGTEDQHLIPGEGLIDWGVVKKKL
                     CEVNYSGAIALEITNEFSKNIDNQDPWAFLKRAYEAAKRLIP"
     gene            37222..37830
                     /locus_tag="PLQ68_00695"
     CDS             37222..37830
                     /locus_tag="PLQ68_00695"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXK71198.1"
                     /translation="MSIIKSHDNTLYGGNGEFNIELKPLCDEHLPYLYKWCSDLDVLY
                     WTETDEVGEPYAKETVHDIYGGVSQNALCFLIEVNGIAIGECWLQKMNMPNVIAMYSE
                     YTDVRRIDMAIGEKDYWNRGIGTQLIQMLVDFAFNSEQVEVLHCFCEEYNKRSQRVWE
                     KNGFILEHKIKHAYNQKSNYEYHYVLTRQQYMQNRHVKACDV"
     gene            37864..38043
                     /locus_tag="PLQ68_00700"
     CDS             37864..38043
                     /locus_tag="PLQ68_00700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK71199.1"
                     /translation="MAINEELLNWGDKKSFPLPKELIKKDLNTSILYIDEIRGGISKQ
                     VYKITTQLNKYILYI"
     gene            complement(38201..39475)
                     /locus_tag="PLQ68_00705"
     CDS             complement(38201..39475)
                     /locus_tag="PLQ68_00705"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015513810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HXK71200.1"
                     /translation="MVRAIVGANWGDEGKGKMTDMLAVESDYVVRFQGGNNAGHTIIN
                     EYGKFALHILPSGVFHKHITNILGPGVALNISVLMDELKNLKDKNVPDPKLYISERAQ
                     VITPWHITFDKLEEERLGSKSFGSTKSGIAPFYSDKYSKIGIQVCDLYSPETLKEKVF
                     NICAVKNVILKHLYNAEILNSDMVFEELLSQGDKIKHMICDTTILLNKVINQGKNILL
                     EGQLGALKDPDLGIYPYTTSSSPLAGFGAVGAGIPPYKINKITAVIKAYSSCVGAGPF
                     VTEIHGDAASVLREKGGDSGEYGATTGRPRRMGWFDAVATRYGCMVQGATEAALTLLD
                     VLGYLDEISVCTGYEIDGTIIEEFPVTSILSCSRPVYKVLPGWKCDISGIRHFHNLPE
                     AARNYVRFIEYSIGVPVKWISNGPKREDIIKI"
     gene            complement(39595..40581)
                     /locus_tag="PLQ68_00710"
     CDS             complement(39595..40581)
                     /locus_tag="PLQ68_00710"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019533718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HXK71201.1"
                     /translation="MKYDMRIFCGSSSVDFTNKICNYLNIIKGDSDVITFSEGNTYVR
                     INEPVRGKDVYLVQSIGLRPNDEFVEILFWMDAFKRASAKSVTAIIPYFSYAKGDKKD
                     EPRVSIRARVCAECIELAGADRIITMDLHSPQIQGFFKKPVDHLQAMLLFCEQIKLLN
                     LENYVIVSPDAGYAKQAREYSKYLGVSTVIADKARVGHNEKADILDVIGDVKGKNIVI
                     VDDFIISGGTLIDLSYKLKKLGAQKIISCTSHILSNEKSIQQLDRSEISMFITTDSIY
                     NPSINLSSKIKVVSAVPLFSEVILRIHNNISISSLFNALPEKAIKISDRHNY"
     gene            complement(40685..41575)
                     /locus_tag="PLQ68_00715"
     CDS             complement(40685..41575)
                     /locus_tag="PLQ68_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002600298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HXK71202.1"
                     /translation="MDVNLELYKVFYYVVRAGSVTKASQELYISQPAVSQSIRQLETK
                     IGGQLFIRTPKGITLTPEGEVLYKYIEQGYNMILLAESKFSEAINLDAGEIRIGASDM
                     TLKYFLLPHLEEFHKHYPKVRIKVTNGPSPETVACLKNGLIDFGIVSLPLFDDSATDV
                     IEAMEIQDCFIANYKFGHLASNNISLKELLNYPVIMLEKNTSTRRYIDHFLYSYGITI
                     IPEIELATSDLLVQFARRGLGISCVVKNFAENDLENGSIFELDLIEKIPPRQIGIIKL
                     KNTPLSAAAVRFTELINSKA"
     gene            complement(41816..41906)
                     /locus_tag="PLQ68_00720"
     tRNA            complement(41816..41906)
                     /locus_tag="PLQ68_00720"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(41869..41871),aa:Ser,seq:gga)
     gene            complement(41956..43137)
                     /locus_tag="PLQ68_00725"
     CDS             complement(41956..43137)
                     /locus_tag="PLQ68_00725"
                     /EC_number="1.1.1.261"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-1-phosphate dehydrogenase"
                     /protein_id="HXK71203.1"
                     /translation="MQFKDLEIKELIKNTYPCECGLVHDPGMERIEISENGIEEAIKF
                     ILEKAKGRIMVISDEITFKIAGGRVFEKLKEKNKDTLSYIIPQKNDVAVVPNESTIGE
                     TLIRMPLEIDFFVAVGSGVVNDIVRNISYHLGKKYVVIGTAPSMDGYASITSALIINN
                     LKESCPGQVPIGIFADINILKNAPYTMLTAGFGDVLSKYNAVREWRFSRDLNKEHYCD
                     EIAGLIWKAADKCSDSAALLKQRDKKAVENIMEALILSGIAMGMYTNTRPGSGAEHHL
                     VHYWDVEFIKRGWEHPLHGNSVGVALCVICRLYDIVKDKLPIKVMDIETEKIEEILKN
                     AGCETDPKALGIPEDIFKDSIIYGHIMSTTKYTVLTHLNKYDKELLEKCSQKLTDYYY
                     S"
     gene            complement(43146..44285)
                     /locus_tag="PLQ68_00730"
     CDS             complement(43146..44285)
                     /locus_tag="PLQ68_00730"
                     /inference="COORDINATES: protein motif:HMM:NF017495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 family protein"
                     /protein_id="HXK71204.1"
                     /translation="MVLTVIQLIIFFGIPYLLINLQEKVKFIKTIGPILFCYAVGIIW
                     ANSGFPLDASLSDTVSSVLIPIAITLILLNSDFLTIKKNVKKTGLSFLILCISILSIA
                     ALSVVVFPNVYENTKIASMLVGCYTGGTPNLFAIGYGLNTDKNTIIVANLADQVMGGL
                     YFLFICTLAKPILTKLTKQVSVAAKKTNYEKESIISKTSLSTKEKIKQGVVALLIGIG
                     ALGASIGISFLLTKQATNVAVIMVSVSVIAVTLSFVKKIRQNQISYPLGQYFIYVFSI
                     AVGSLADIKLIVGQSVQVLGMVAFVLFATMLLHFIISLLFKIDVDTIIITSIAGIFGP
                     AFIIPTAKAIKNEGVILIGIATGLLGYAIGNFMGFGLYELLKLIN"
     gene            complement(44317..45711)
                     /locus_tag="PLQ68_00735"
     CDS             complement(44317..45711)
                     /locus_tag="PLQ68_00735"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HXK71205.1"
                     /translation="MIKELYQPRYLLRKQRLLGKVPTAKEAYGRMFRMAFPSTVEALL
                     GGLTSFVDTIMVSAIGISAVSSVGITTQPRMIILMLFMALNTGVTALIARRRGENDAK
                     KANQILMQSLLICLILAIIMTVVGIATARPLLKFAGAGDDIIDDAVIYYIIVTAGTVL
                     MALTMTINAAQRGAGNTKVAMKTNIAANIVNVVLNFLLIKGRLGFPALGVKGAAIATF
                     LGILTAFVMACLSLRKSTFIFFNFKNLKNFDLKLVKSIIKISGNAAIEELCLRVGFFM
                     YNKIIAELGTVDYGTHLVCINILNLSTNIGSGLGIGTTALVGQTMGEKRSDLSVLYVK
                     IGQRMAFIISIALSSLFIFGGKMLMGLFTDDPSIIANGSKILIIMAAVTFFINSQIVY
                     SGCLRGAGDVRYVATMALISVAVIRPGISYLLTYPLGLGLIGAWLGLFSDQLVRGMAG
                     RIRFKMGKWTKINI"
     gene            45811..46761
                     /locus_tag="PLQ68_00740"
     CDS             45811..46761
                     /locus_tag="PLQ68_00740"
                     /inference="COORDINATES: protein motif:HMM:NF012475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HXK71206.1"
                     /translation="MKIQKVFMDNMQSYTDCFKKVFEIQGRYVNDHCFIIHNNEIHLF
                     YIDGETGKGCYDIGNEIIIGHAVSKDLISWEKKQPALVYDQSLSFEERGIFAPYVYKK
                     ESRFYMFYSSHNIKKAQFICLAFSDNLNTWQRYERNPLFKPSSDWIFWDDSVPCSCRD
                     PHIFYDEDKKEYILYWVADIKENNKLSCIASSISNNLFSWQETGPVLIREHSFYESIT
                     CKTESPCLIKKDNLYYLFYRHGNGTKYCISDSYTNFTHSDSYYLGPSHASEIFMFKNQ
                     WYISSCSRKIADLEHKTDRSKGLFLARLSWNGLHPEIYSL"
     gene            complement(46849..47907)
                     /locus_tag="PLQ68_00745"
     CDS             complement(46849..47907)
                     /locus_tag="PLQ68_00745"
                     /inference="COORDINATES: protein motif:HMM:NF014381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD/NADP octopine/nopaline dehydrogenase family
                     protein"
                     /protein_id="HXK71207.1"
                     /translation="MNKKIAVISTGNGGQAIAAFIALKGYYVSLYAREKERVDMFANN
                     LFHIKGIINDVASIGLISCDMEEVIKDAYLILVTTPAQYHFAVAKEMALYLKTGQVVV
                     LNPGRTFGTYEFNQVLEQNGCNADILLGEADTLVFTCRCDKPGNPHIYEIKKKVKIAG
                     HKKAYTKEITDIMNEIFHDMESAPSVLHTGLSNIGVIFHPLPTLMNITRIEAKEVFRY
                     YIDGISPLVANILEELDKERLETARKAGIELLSVCEWLKERYGSTGNNLYECIQNTHA
                     YSSVLAPFDIDTRYIHEDVQTGCVPISCLAKEMGVKTKIMDSAISWASIIYKKDFYEC
                     GRNNKKLDIRKIIDDNLK"
     gene            complement(47908..49554)
                     /locus_tag="PLQ68_00750"
     CDS             complement(47908..49554)
                     /locus_tag="PLQ68_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HXK71208.1"
                     /translation="MKKGKIIGASVGNCVHVAGVIHFLSLAQDEGYDTVFLGPAVRTD
                     VLFEAIKLNSPRYVAVGYRLTAENFEKLLPEIINLNKKLEKKPLWVFGGTPPVAEIAE
                     KSGFFDVVFDGTEDIDDCISFLRTGIYSKDTNIEHYAQDIVSRIEQKKPYPLLRHHFG
                     LPSYEETKKGIETIAKAKVLDIISLGIDQNTQQYFFRPEMRNKDMDGAGGVPVRTKEQ
                     FEDLKKASRFGNFPLMRCYSGTDDVINFADTLHQSISNAWCAVPLCWYNEIDGRGTRK
                     LHVSMDDAQRLMRWHGERGIPVELNEPHHWGLRDAHDVVSVAMSYISAYNAKKNSVKD
                     YISQYMFNIPNSLTFSMDLAKVLAQVEMTESLKTNEFRVYRQVRAGLPFLSSDIDIAK
                     GQLAASTQLSMAIEPHIIHVVGYSEGLKAASADVVIESCKIVRGVVRSVLYGNADAKQ
                     DLKVQSRKRELIIEAEYLIKFIEKLYEDKSEDPLADAAVLTDCIKKGILDAPHILKND
                     KYTGTLNTRVYEGKCLAYDPKERCFLNEKQRLFRLTGKEI"
     gene            complement(49570..49911)
                     /locus_tag="PLQ68_00755"
     CDS             complement(49570..49911)
                     /locus_tag="PLQ68_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019640047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HXK71209.1"
                     /translation="MKLILAVIQEADVKNLLSKLIEKKYKATKLASTGGFLKARNTTL
                     MLGVEENRVDGALRIIKENCKKRRVLDPIPPYSIGSHGEYINMDPVEYVVGGATVFVL
                     DAFDYSTEIDK"
     gene            complement(49913..50566)
                     /locus_tag="PLQ68_00760"
     CDS             complement(49913..50566)
                     /locus_tag="PLQ68_00760"
                     /inference="COORDINATES: protein motif:HMM:NF014171.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA C-terminal domain-containing protein"
                     /protein_id="HXK71210.1"
                     /translation="MPNQRINPQYQMIARDIAKRITDNEWTENQMISGRSLLSSEYKV
                     SPETIRRALALLVDMKVVYVKKNSGVNILSKDNAKRYLSSFDEMKDQMDMQSELKDLI
                     EKNDEINREMHDLLAKIIKLQSTISYSNPNIPYYEIKVSLDSSIVGKNIEDIKFWQNT
                     DATIVAIRRGKNLIISPGPKAEIYENDTVVIVGSQKAVQKAADYINGKTSVLQGGGN"
     gene            50893..52215
                     /gene="dnaA"
                     /locus_tag="PLQ68_00765"
     CDS             50893..52215
                     /gene="dnaA"
                     /locus_tag="PLQ68_00765"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HXK71211.1"
                     /translation="MTVQEIWNDALVNIESVMGELNFKTWILPLEPYSIDGDNFVLVT
                     QSDFSRNYLTNYKLLLKNAISDAAAKDLSPLFKLKNEIREYEEKGRTFMPKNNTYMDS
                     QLLDKYVFSNFITGSSNQFARAAAEAVVDNPGTTYNPLFFYGESGIGKTHLMHAIGNA
                     IAEEFPDKKILYVQSETFTNEMISAIKTAKSKEFREKYRNLDVLLIDDIQFITGKEGT
                     QEEFFYTFEKLYSANKQIVITSDVPPEKMSRLEERLKTRFQQGLMADMQTPDYETRVA
                     ILEQNSLKSEYNISKDVIEFIAKNISSNVRNLQGALNKTVAHARVFGDDITIDLAKKA
                     LKDMIDENNVNDITIPSIIDTVARYYNITTKDIISPTRKSNIAFPRQIAMYICRIKTS
                     YSLPVIGKYFADRDHTTVLYAVDKVAEKIKTDKEVENNVNELLDKLSY"
     gene            52417..53517
                     /gene="dnaN"
                     /locus_tag="PLQ68_00770"
     CDS             52417..53517
                     /gene="dnaN"
                     /locus_tag="PLQ68_00770"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HXK71212.1"
                     /translation="MNFTCTRKKIQEAVNSVSKAISNNPIMPILGNIFVKAKDSQIIL
                     RGYDLEIGIEHIENAQVEEEGEVLVNSRLFSDIVRNIGEETVNIYTKDSNLVIISGNA
                     VFNIRWIESSSFPKLPDVEKENKVTIKAEYLKNMIRNTVFSVSTSEQRMILTGALFES
                     DDNYFQMVTLDSFRMSIRKEYILGNEEKFKFVIPGKTLNELLKLITNDDEEIKVFFET
                     TQVLFEFGNCKFISKLLQGEFFNYKAIIPKDFVTKIEIDVNEFREIIERNMLVSDDGR
                     RAPLVLDIKDNILTANTQGESGNVTETQIIKKDGKDMRIGFNAKYLLDAIKSLEEEKV
                     RISFMGETNACVISPLNTDEFLYLILPVRIAS"
     gene            53527..54633
                     /gene="recF"
                     /locus_tag="PLQ68_00775"
     CDS             53527..54633
                     /gene="recF"
                     /locus_tag="PLQ68_00775"
                     /inference="COORDINATES: protein motif:HMM:NF000823.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HXK71213.1"
                     /translation="MFINNITLKDFRNYEYQEIFPKKGINIIYGRNAQGKTNILESIF
                     LCSAARSHRTNKDTDLIRNDQKGYYVQIEGKRDEQPFSISINCDKVSSKTNKRVIKVN
                     DISLTRTGQLMGVLNCVMFSPEDLTVIKEGPSERRRFLDILISQINPSYFYALQEYFK
                     IIKQKNALLKNISENGRNKELLDVYNIKQAKTGYKIIKERKIFIEQINKKSKINHSDI
                     TKGSEELNISYSCDLSDFSDGEDEFYRMLTKSADKEIKIQNSILGPHRDDIDIILDNM
                     SIKEYGSQGQQRTAILSLKFSEMQIIKEKTGNNPVLLLDDALSELDLLRKRTLLDKIN
                     TNQTFITCTEKRKYMTLDSNISFFSVTKGTVKSI"
     gene            54686..54955
                     /locus_tag="PLQ68_00780"
     CDS             54686..54955
                     /locus_tag="PLQ68_00780"
                     /inference="COORDINATES: protein motif:HMM:NF015958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF370 domain-containing protein"
                     /protein_id="HXK71214.1"
                     /translation="MFLHIGDDLVLREQDIIGIFDFETSTLSKHTKKYLQDKEKKHST
                     LAVSEQIPKAFVVVQKKEQKEPKVYFTLVSSITLKKRAENNRLME"
     gene            54959..56902
                     /gene="gyrB"
                     /locus_tag="PLQ68_00785"
     CDS             54959..56902
                     /gene="gyrB"
                     /locus_tag="PLQ68_00785"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015357739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HXK71215.1"
                     /translation="MENNKRQENIKACNYNEAQIQILEGLEAVRKRPGMYIGTTSVKG
                     LHHLVYEIVANSVDEALAGRCDKIEIFIDKDNIITVVDNGSGIPVGIHPKMGIPTVEV
                     VHTVLHAGGKFDSKAYNISGGLHGVGASVVNALSEFLEVEVKRDGKIYRQRYERGKTV
                     TPLEVIGKTDETGTKTVFKADNQIFDTVEFEYDALLSRYREMAFLNKGVMIILTDRRG
                     DEEVSNELYYEGGIVSFVRYLDGNKARIHKNTIYIEETKEDCIVEIAMQYNETYSETL
                     YSYANNIATIEGGMHVTGFRSALTKVINDYARKIGALKESNKNLTGDDVREGLTVVIS
                     VKLHDPQFEGQTKSKLGNSIIRTITEQTVNEDLAAYFEENPQEGKAIVDKCLLASRAR
                     EAARGARDLARRKTALEGNALPGKLADCSETDPVKCEIFIVEGDSAGGSAKQGRERKY
                     QAILPLWGKMLNVEKARIDKIYNNDKLHPLITALGTGIGEDFNIEKLRYHKVIIMADA
                     DVDGAHIGMLLITFIYRYMRELIEKGFLYLAQPPLYKITKGKEFVYAFNDAEMERIFR
                     DKGWDRKECSLQRYKGLGEMSSTQLWDTTMNPETRTIVQLKMDNPFLADIAVSDLMGD
                     DVQPRKEFIERHAKDVQLNRLDI"
     gene            56934..57707
                     /locus_tag="PLQ68_00790"
     CDS             56934..57707
                     /locus_tag="PLQ68_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018663488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HXK71216.1"
                     /translation="MGKVMAVANQKGGVGKTTTSINLSASLAYMGKKTLLIDNDPQGN
                     ATSGLGIDKWTVEMSVYDAIINDIDIKETILPTMMDNLYICPSNIQLAGAEIELVSQM
                     TRENRLKNAIATIKEQYDYIIIDCPPSLGLLTLNSLTAADTILVPIQCEYYALEGLKQ
                     LMKTVSLVQKHLNPSLKVEGVVMTMYDSRTNLSFQVVESVREHFSDRVYRTIIPRNVR
                     LSEAPSHGVPIILYDKDSKGALSYLDLAEEVIQCGGRNG"
     gene            57700..58533
                     /locus_tag="PLQ68_00795"
     CDS             57700..58533
                     /locus_tag="PLQ68_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012061243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HXK71217.1"
                     /translation="MAKYGLGKGLGALLDISDDNNKNQLIELDIDKIQRDPKQPRKEF
                     SKEELDELAESVREKGILQPILVRKTGDVYTIVAGERRFRAAKQEKLKKVPVIVKEFT
                     EKEVLEISLIENIQRKDLNPIEQAEAYKRLSDEFGATQEEIAKSLGKSRPVVTNKLRL
                     LTLSERILSYLRRGKLTEGHARLLLSINDDLREDIAQEAAEKGLSVRELEKLIKKAQT
                     EPRKKEEIENEEEFIKVENRLQKKLATKVSISRSIKKGKITIEYYSLEGLNRIIDILS
                     K"
     gene            58555..59043
                     /locus_tag="PLQ68_00800"
     CDS             58555..59043
                     /locus_tag="PLQ68_00800"
                     /inference="COORDINATES: protein motif:HMM:NF025935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4446 family protein"
                     /protein_id="HXK71218.1"
                     /translation="MEYLYYGLISLAVSAVVSYLIIQIFLYNKKRRDLKKEIKLDDIK
                     TYEDMLYQINKLYKKDHEIESKIRFINNRCDKLKENATYAIKNIGLVRYKYSDDAGGN
                     LSFSLALMDDNQNGLVFTNISLIDSNSLYVRRIREGISDVLLMEEEKEAIKQAIEKVE
                     QN"
     gene            59141..59231
                     /locus_tag="PLQ68_00805"
     tRNA            59141..59231
                     /locus_tag="PLQ68_00805"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:59176..59178,aa:Ser,seq:tga)
     gene            59235..59326
                     /locus_tag="PLQ68_00810"
     tRNA            59235..59326
                     /locus_tag="PLQ68_00810"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:59270..59272,aa:Ser,seq:gct)
     gene            59500..61329
                     /locus_tag="PLQ68_00815"
     CDS             59500..61329
                     /locus_tag="PLQ68_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HXK71219.1"
                     /translation="MYEVIVIGAGLSGLTAGALLCKKGIKTAVIDKNYMPGGSCGIFK
                     REGVTFDQGSAMLFGFGEKGFNAHRFVFNCLEQPIDMIKHDLLYVMNFNGHKIRFYAD
                     LDLFKEELSSVFPDQKDNIVRFYDDMSTMYQHVLVETPSYTTPDENDLDDAKEGFKKH
                     PLSYIKFLSYLNISAEKLLKRYFDNDEIIKFFDKLTSTYCYTTTKESPAILASVMFVD
                     NHTGGSYYPAGSTLFLPGLLEKSIEENGGDMILEKEVTKILFENNKPVGVELNTKEKI
                     HADNIIFSGTVWDLYGKIIDSNETTEKKRRGARNMIPTYPSVVLYCLVKDDCIPKDTQ
                     PVEMLVGNVDQIDESEVTAYIFSIDDKTLCEEGYHVIAAIGPTFVKWSELNEEEYITR
                     KEQEKERLIKVLERRFPDIRNSLVYTNLATSKTLERYLNKKDGAVAGPKQKLGQHMFK
                     RQHTKSQWDNLYCCGESTVMGTGTPTVTTSGISAANAILKKLGKDKFKYDINIVNYVN
                     VIGKPVKNDDLFYKYDDETAKIVKKAFACQLCENPTCAEPKEFDVRGIMRRVCVGNFI
                     GAKKLINEEISVEKLKQYEKMCIEKKRINKSVEISDVIEFLRK"
     gene            61331..62938
                     /locus_tag="PLQ68_00820"
     CDS             61331..62938
                     /locus_tag="PLQ68_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018963777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HXK71220.1"
                     /translation="MNNKNYDVIVVGGGIAGITATAYLSKYNIKTVLFEKNEKVGGLI
                     KTFEYKGFKYDGGARAYENSGVIYPMLKQLGIVLENVHNPVSVGIGDNIIEFKDKNSL
                     KDYQDLLSNNYPDNVDEIHEIIKEIEKVIDYMEVIYGIDNPMFSDNFTDKEYVFKQLL
                     PWLMKYTVNIKKAQKLNEPVNEYLLKFTKNQSLIDAITQHFFKGTPTFFALSYFGQYL
                     DYSYPIGGTGILIDKLSEYIKSKNNEILTENEIVSVNTISKTVTTKDNKEYSYKYLIW
                     AADLRSLYLNAKIDNLKKSEIKKIEKIKNKVLSNKGSDSVLSVFINTDLENEYYRKII
                     EGHCFYTPDKRGIGTSMMDKRDVFLKDKSEKDEFETLKEIVYEYLKLTTYEISIPSLR
                     DASMSPKNKTGIIVSTLIDYGLFNYAKEKGWYEDLKEYCQKCVIDILNESIFDGFKTK
                     VTEATCSTPITIEKVTNGFEGSITGWAFSNNMPVQSELTKIAKAIYTEIPNVYKAGQW
                     SFYPSGIPTCIITGKLAADNIKKKMRK"
     gene            62940..64025
                     /locus_tag="PLQ68_00825"
     CDS             62940..64025
                     /locus_tag="PLQ68_00825"
                     /inference="COORDINATES: protein motif:HMM:NF024896.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HXK71221.1"
                     /translation="MRYNIVLISVGLILSIILYFRFPLLAKKENIEGKYKVSVIIPAR
                     NEEKTLPFLLSDLNNTDMILHEVICVDDSSTDNTKQVILDNNCILVEVNDKPDNWLGK
                     SWACQKGADRATGDVFLFLDADVRINKQGITKLISQYDKDKSVISVQPYHKMEKIYEQ
                     LSFFFNIVAIGGNGAAYAFKDGSAGLFGPVILISKKDYIASGGHHGAKEFILEDLAIG
                     QVLRARGIKYNLYIGKKDISFRMYSQNILQIIEGWTKNYAAGASMAKPAVFLMTFLWI
                     TSLCATAVNFITSWVTFEPVKIIIFTTFYIIWVFELIRISKKIGNFSVLTCILYPVFM
                     LFFVMVFLFSVFKKIFKIRVTWKGRKI"
     gene            64025..64519
                     /locus_tag="PLQ68_00830"
     CDS             64025..64519
                     /locus_tag="PLQ68_00830"
                     /inference="COORDINATES: protein motif:HMM:NF040142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl-4,4'-diaponeurosporenoate
                     acyltransferase"
                     /protein_id="HXK71222.1"
                     /translation="MRLLILPDIWTILLCIPLWFIFHTLSAYICLKIPDKYLNPDGFL
                     FKTRKWENNGLIYDKVFKVRKWKKYLPDGGAMFKNGYSKRYLKDYSEASLKKFVVETC
                     RAELTHLLAILPFWIFGFIAPPIIILYMLIYALAANLPCIITQRYNRPRILSFINKIN
                     EKNT"
     gene            64565..66013
                     /locus_tag="PLQ68_00835"
     CDS             64565..66013
                     /locus_tag="PLQ68_00835"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HXK71223.1"
                     /translation="MSFNSLPYLIFIICVCLLHYLVRPKYRNTILLAASYFFYVYIDP
                     RYSVFLIFSTLMSYMFARLIQKDIQKRKKFWFIISILVSIGLLVIVKYLGFFTSSISS
                     LLANLQLPVLPNLNIIVPIGISFYSFTVTGYLIDVYRNKVTAEKNIIDYALFVSFFPQ
                     LLSGPIERASNMLFQYKRTRKFSFENLKTGMIRFLWGLFKKMIIADQLAIIVNNTYSV
                     LPNSSSLNVIIAIFAFSLQIYIDFSAYSDMALGSAKLLGLDIMENFDSPYLSSSVRNF
                     WRRWHISLTSWFKDYLYIPLGGSRCSNIRHLLNIFIVFAISGLWHGASFTFLIWGIIN
                     GIYLIIGQVLSPVYSKIKEKVKINENNRLFLLIKGVFTFMLISFSWIFFRSEDIEQAF
                     LVIKKGFTSSQPLLNYQQIGISLSNMIVLGASLLLLSYVDVFTRKNDLISEINKKLWL
                     TYAICLALIISVLIFGVYGPGYDPIDFVYFKF"
     gene            66313..66447
                     /gene="rpmH"
                     /locus_tag="PLQ68_00840"
     CDS             66313..66447
                     /gene="rpmH"
                     /locus_tag="PLQ68_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004815746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HXK71224.1"
                     /translation="MKRTYQPKTKQRSKEHGFRKRMSSAAGRKILKNRRDRGRKRLSA
                     "
     gene            66486..66845
                     /gene="rnpA"
                     /locus_tag="PLQ68_00845"
     CDS             66486..66845
                     /gene="rnpA"
                     /locus_tag="PLQ68_00845"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HXK71225.1"
                     /translation="METLKKNYEFSRVYRKGKYFSGQYLTINVLENIYGYSRIGISVS
                     KKAYKSSVKRNLYRRRIKEIVRTGCFNLKQGYDLIFVVKKTEQIPPYREIEKEIIDLL
                     EKAKLILKNEENNNQVD"
     gene            66817..67026
                     /gene="yidD"
                     /locus_tag="PLQ68_00850"
     CDS             66817..67026
                     /gene="yidD"
                     /locus_tag="PLQ68_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003451050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HXK71226.1"
                     /translation="MKKIIIKLIKFYRINISPNKLPCCRFVPTCSQYALEAVEKHGAL
                     KGSLMAIWRILRCNPLCKGGYDPVK"
     gene            67041..>67722
                     /locus_tag="PLQ68_00855"
     CDS             67041..>67722
                     /locus_tag="PLQ68_00855"
                     /inference="COORDINATES: protein motif:HMM:TIGR03592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HXK71227.1"
                     /translation="MFDFIYNLFGKFFELLYTGLCAENFGVTLIIYTLILKLLLMPLS
                     IKGTKSTMRMSELTPKVDAIKKRYKNDQQKQNEEIQKLYKQENVGCASGCLPTLIQFP
                     LLIAMYRVLSQPLTYIKGVSKEVITQIAERLGYGATVLQTTINNDLITDPAKMELVKD
                     LITPDKLIDMKFLGMNLGLVPKINPSILFGAETIGAYLPILFLPLISVGLVILSQYLT
                     EKAMKSRMP"
     assembly_gap    67723..67822
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            67898..69268
                     /gene="mnmE"
                     /locus_tag="PLQ68_00860"
     CDS             67898..69268
                     /gene="mnmE"
                     /locus_tag="PLQ68_00860"
                     /inference="COORDINATES: protein motif:HMM:TIGR00450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HXK71228.1"
                     /translation="MEHTISAISTPIGVGGISVIRISGHNAFSVAKGMFKSKTPFDKI
                     KSHTVTYGKIHDNKGNTLDNVLLLKMKAPKTYTKEDTVEIHCHGGIYITKKILELTYE
                     NGALPAQAGEFTKRAFINGSIDLMEAEAVMDLINAEAEDAYKDALAQLKGEKSIEIKM
                     IRNELIDLISHIMVIMDYPEYDIEKLTVDQAKKQACIIKKQLKELIEDFDNRRIIREG
                     LNVAVVGDVNVGKSTFINRACGQNRVIVTDIAGTTRDVVEVKIDVKGLCVTLKDTAGI
                     RTTEDEIEKIGIEKSKEAIEQADAVIYIADINRPKKDEIFNYIKNLDKTICVLNKTDL
                     ITENEREQHISNIEKDLQKTFKDRIISCSLDKKEGINEIFDKLLDMVDAGSIRQGTSI
                     ITNARHRKHILDSIEFLENVEQGSYIDMLSVDLTCAADELGKITGETASSDIVDRIFQ
                     KFCVGK"
     gene            69270..71159
                     /gene="mnmG"
                     /locus_tag="PLQ68_00865"
     CDS             69270..71159
                     /gene="mnmG"
                     /locus_tag="PLQ68_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014257141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HXK71229.1"
                     /translation="MINYDVIVVGAGHAGCEAALASARMGMKTAIFTLNLDSIANMPC
                     NPSIGGTAKGHLVREIDALGGEMGKCADATLIQSKMLNRSSGPAVFSLRAQIDRRQYQ
                     IKMKHILEMQENLDIRQYEVVDVVFENNKVKAVKTNTSAVFGAKAVIIASGTYLKARI
                     IIGETSYEGGPDGLFPANLLSVNLVEKGMKLKRFKTGTPARVHKSSIDFEKMSIQNGD
                     EPIVPFSFETESIDINQEPCYLTYTNKNTHEVIRNNLHRSPLFSGQINGTGVRYCPSI
                     EDKIVKFADKERHQVFIEPMGLDTEEMYVQGMSSSMPEDVQEEMYKTIKGLENVKFMR
                     SAYAIEYDCFDPLQMLATLEFKDYEGFYSAGQSNGSSGYEEAAAQGIMAGINAALKIK
                     GMPELVLDRSEAYIGVLIDDLVTKGTNEPYRMMTSRSEYRLILRQDNADERLTPKGYE
                     IGLISKERYDKFLTKMDNIEREIERLKNKNVSPKIANPFFEKRNQQTISSGITQSAML
                     KRNGISYTDFCEIDDEMPPLSNEEKEEVEIKIKYEGYIEKQLRQVEQFKKMERIHLSR
                     DIDYSKITGLRNEAKQKLNSMKPASLGMAGRISGVSPADIQVLYVYLEQQKRKMKESI
                     DEASS"
     gene            71143..71841
                     /gene="rsmG"
                     /locus_tag="PLQ68_00870"
     CDS             71143..71841
                     /gene="rsmG"
                     /locus_tag="PLQ68_00870"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HXK71230.1"
                     /translation="MKHLLKGILDYAGLEYKETIDIMFSSYADMLIEWNKKFNLTGIT
                     DLNDIYIKHFADSLILYKGIKSASSLIDIGSGAGFPGIPLKIAGYDKKVVLLEANGKK
                     TMFLDHVINSLKLKDISTCLSRAEVAGRDSSLRDSFEIATARAVAPLNVLLEYCTPFI
                     KKGGVFIAMKTDESELNIAKNAQKQLGVEVCDIDMQILPFSDMKRCNIYFKKIKNTPD
                     RYPRADGIPKKKPL"
     gene            complement(71950..72675)
                     /locus_tag="PLQ68_00875"
     CDS             complement(71950..72675)
                     /locus_tag="PLQ68_00875"
                     /inference="COORDINATES: protein motif:HMM:TIGR00696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HXK71231.1"
                     /translation="MDILGLRIDDIDMEEALKKAYDAIIIKDNFKYIFTPNAEICMAA
                     LKDKRLMTALNSSYMNIPDGEGVILASKILKTPLKEKVPGCVFVQNLLNLKKSFSVYL
                     LGASEESVIKAKENILKKYPHINISGYRNGYFVKDDVEGIIDDINTAQPDVIIIGLGA
                     PQQEFFIYDNRNRIKSGLAIGVGGTIDILSGYAKRAPEFFCKYKLEWLYRLAKQPKRF
                     KRMLSIPAFLIKCKIHVMKKKKD"
     assembly_gap    72923..73022
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <73023..74086
                     /locus_tag="PLQ68_00880"
     CDS             <73023..74086
                     /locus_tag="PLQ68_00880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK71232.1"
                     /translation="LYRSLTSYEQVGETVTLKNKKYNRVYLTEEAQLDMSKVEIYNAD
                     FDQAVVVKDSSSLAILNSQILSKNTAITSVGEETIINAISSEMASYGLFVLDISDKAL
                     FTGSNLKITNLSENGSGINVRSEASMVINNSNVSVKGVGVETDAIVSISNSEITSSSL
                     KFYEGASVTLTDSRFYTDHGLLLVDNPTENKIHVSLNLEKVKLNTTYGAALSMIDAKA
                     SVKISESTIGQNSKNVMSLSNSEGTLTLCNTSIEGNINTDETSSLNILLKQESVLIGQ
                     INNENKYNTVTIQIEEASVWELTQDSYVRGIILKDKTLNGIKSNGHAIYYDTKCATNT
                     WLNGNTIYLEDGGKITPDN"
     gene            complement(74093..74764)
                     /locus_tag="PLQ68_00885"
     CDS             complement(74093..74764)
                     /locus_tag="PLQ68_00885"
                     /inference="COORDINATES: protein motif:HMM:NF039343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK71233.1"
                     /translation="MMIDIHSHILPKADHGSHNLEESLKQLMTASKYGVKGIVATPHF
                     YPFNDSIEKFLKRRDKAFDELSAANSTGIKIYKGAEVRLTTGLSSLKHIKKLAFENTN
                     YLLLEMPSSTTWNNILLNEIIKLQSLGLTIIIAHIERYSSNQINKLMEFDVLTQINAY
                     SLFSPIKWFRIRKYIKSNVVTFIASDSHIYNYFFAYKYLKKSHNMIGSKFNTYMNNAK
                     NILSL"
     gene            complement(74754..75500)
                     /locus_tag="PLQ68_00890"
     CDS             complement(74754..75500)
                     /locus_tag="PLQ68_00890"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HXK71234.1"
                     /translation="MADVERREFLLFDNSSFPVTEAIKATRTNLMFTTAKEKCPVISF
                     TSAHMGEGKSTVCANMALAFAEAGFKTLVIDADMRKPTLNKYFKIKSSPGLSDILGGL
                     VKENFIYPTKYKNLHVLPSGTIPPNPSELLTSERMSILLNELRETLDYIFIDTPPILI
                     VTDAIALSNKVTGTVVAVRQNFSRTNLIKEAVDALNNVNGKVLGFIFNDYDIESMAYN
                     KQYKYGKYERYGGYDKKPVKSASPVSLDDD"
     gene            complement(75478..76200)
                     /locus_tag="PLQ68_00895"
     CDS             complement(75478..76200)
                     /locus_tag="PLQ68_00895"
                     /inference="COORDINATES: protein motif:HMM:NF014732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HXK71235.1"
                     /translation="MEELDVRQIFTYLKRHFWLIICIVLVAASLSATITYFFITPQYE
                     ASATLYVFNDSNRSDTSISTTDITTSQKLIATYSVIMQSDNVIHKVIETTNLRYTPSQ
                     IRNMFTGTSVNNTEVMKVTITSADPVDSMVLANAFIDVAPDEILRVIKAGAVEILDRA
                     EIPQQPSSPSMMKNIAIGAVLGVIAAVAFVILYELFNTKVRNEENLLRVVKAPVIGYI
                     PDLQLYQKDDKEVHSYGRRRKA"
     gene            complement(76321..77511)
                     /locus_tag="PLQ68_00900"
     CDS             complement(76321..77511)
                     /locus_tag="PLQ68_00900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK71236.1"
                     /translation="MDLSTLLKYSRKNLKLIVVVVLCCALLAGGVKMLTDYVTYSKDS
                     QTIPQLQAKLDSLEAQKNALHSDIDKIDNNEEYISVKDMNASRLLKYTAVYYIDRNVD
                     MYNTVINDINVIEKAYSNSNIELSFNVVDMLIDFTANSSYRSLTIHVSAKNEEQLSKL
                     KDIAEQCFSHAKDTIRITLGSSTIHLLSENESFASNSAIANLQKSYTDRISTINTSIG
                     EIDTQITETNDTLSKIQFTLFSKDVFQYTLLGLILGVFLAYVFILGRLYLSPKFLGIE
                     QLMAKISLLASGKDIEKLEKACVVPVIYACGKTEEHIGVLSSGNISHAKRIFSSLKEQ
                     TEYNLMHLENIFNSSDIAKTLLDCKGIILVEKCNVSRVKDIEKIINICKNNNITIYGI
                     IIIE"
     gene            77748..79373
                     /locus_tag="PLQ68_00905"
     CDS             77748..79373
                     /locus_tag="PLQ68_00905"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HXK71237.1"
                     /translation="MSNKKAKKNVEEPKTKKNLLKDFFYLEQDKKKPNYFELITTAFL
                     YVFLSSFALIISRKVYGDMTETKLYTLIAMFSIWLIIMIFGFVEKIRSKKANTLYFKQ
                     AAKNLSVTDIALIAYMLIAFISALISEYRHVAFVGVEARNEGYLMQLMYIAIFFMISR
                     SYIHKLELKIFALASLPVALLGILQLNGYYVGRITYDITTQGTSFLTTLGNIDVVSTY
                     LVTPIMLFTVLYCQSKEKFWRILYLASAAICFYFSIMIGVDSGYVGMAAAIFFMFPFI
                     AKDRISASRMFQILSVFLFVIYLEDQIAQIGNRNFTLFSKPFSEIMFLAAVACLVTSA
                     IIFFIGRYIKIEKKFIWPVTYFVIAFVAAFIVIKKIMAMEEPQGSSVMLVLYQFGQIL
                     NGNIDDSFGSNRIYAWRVVWGFFKEKPILGHGPDTVFDLWLREEYQYSMKEFNVYFDK
                     SHNDYLQVLVTTGLLGFISYLTFQFSLLVRSFKKFNQPLVIALVTASICFCVQAFFNI
                     GVPIVAPYGWIIFGMLASVLRKKDELDEQEMGV"
     gene            79380..81311
                     /locus_tag="PLQ68_00910"
     CDS             79380..81311
                     /locus_tag="PLQ68_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016286853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HXK71238.1"
                     /translation="MVKKIKDNSLVLKKILLVIADTVSINAATFLALFFRYELSFSKI
                     DLQYIIHAQQYLWIDTILTLCIFALFGLYTGLWKYASIKELFNILLACSLSYLVKFTL
                     TSIIGLNQPRSTVFFGWVLLVMMVFATRFSYRFIRYIVHENLNGKDIKRVMVIGAGDA
                     GNMIIREIRSSQNLHSKVVCVIDDDKKVHGLFIQGVKIIGGRDMIVEMAKKNKIDEII
                     IAIPSVGASERKKIYDICKETKCTLKTIPGMYQLINEEVRLSDLREVQIEDLLGRDTI
                     DINVQEILDYVSGKVILITGAGGSIGSELCRQIAMHNPSKLIMLDIYENSIYDVQLEI
                     TKKYPALKIETLIASVRDEKRIKNIMSKYKPDIIFHAAAHKHVPLMETSPNEAVKNNV
                     FGTLTLVKAADEAKVDKFIMISTDKAVRPTNIMGATKRICEMIIQAYDKKSSTDFVAV
                     RFGNVLGSNGSVIPIFRKQIAEGGPVTVTDKEITRYFMTIPEAVSLVLQAGYYAKGGE
                     IFILDMGEPVKIYDLARNMIMLSGRTPDEDIKIEVTGLRPGEKLYEELLMDEEGIEKT
                     QNDLIYIGHPIQFDEKQFFDNLKQLKKMTEEEVSCMKLIIAKMIPTYEPDISDSEGIN
                     SIETAECITRLFAHDNQKM"
     gene            81364..83625
                     /locus_tag="PLQ68_00915"
     CDS             81364..83625
                     /locus_tag="PLQ68_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005586845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HXK71239.1"
                     /translation="MKTDDKIQKLVDSYGSWFNEDSGFKAAKMTNKHAPYPLLFSPIQ
                     VNKLTIKNRLVMAPMGNINMAEETGRPNEKMIAYFTERAKGGVGLITTGLIPISFGID
                     NSLIELDKLTYFPRIDRSRTVYMGWRNLAHSVHSYGARIFVQLTPGLGRVGNPQCLTN
                     QFKFPVSASLNPNWYMSEVPCMPLTDAACKKIIKRAGQAAADAKAFGLDGVYLHGHEG
                     YLLEQMTNPAFNRRKLGRYANWQAFGLDMIEEIRRRTDNAFPIMYRIDLSLALNETYG
                     KTMDINALKKFKNGRTVEMTLDYMKNLVSKGVDMFDVDLGCYDNWWLPHPPEGMPPAC
                     FSDISRIVKKYFDESNILSNAGVKIPVVAVGKLGYPDVAEEVLMQEKADMIMLGRPLL
                     ADPKWPLKAKEGRTDDIIPCIGCQEGCINEFVEGGHPQCAVNPRTAFENLYPEEPKQA
                     DKKKKIAVIGSGPAGIMAAVTAAKRGHEVVIYEKQNVIGGRLNAGGRPKIKFDIQNYH
                     EYLKKLIERTSKEYKLKLVLKTEANAELLKKEGFNAVIYATGTKDVELKLPGYEKANK
                     VQAVDVLDNPKIVDKANKVVIIGGGVVGCETAFFLNKELGKEVTVIEMEPYFMNHVCT
                     ANRAYLIHYMEKADIKLLNCTKMLSYEINGVKVSRNVSKTVPNPYKTWNPILPANIEN
                     PLAKKIKEEYEEQFIEADIVIQAAGGRCDDSGFYALQKEMSTCEMYNIGDSFKGGKVF
                     EATKSGYAVGLSV"
     gene            83648..84928
                     /locus_tag="PLQ68_00920"
     CDS             83648..84928
                     /locus_tag="PLQ68_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003869930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase X"
                     /protein_id="HXK71240.1"
                     /translation="MKLTEDELAILNGEKGEVMAKVMKSLVMYGDVFNAKRMVEISAP
                     SHFVTSFGISIMKAVFDIMDEINNAGITTIEKFTVDPRPLDYDNVKCNPLEKLVFGIM
                     YGKQEDYEEQLKKTGLKDSNAFSCTCYMEEVGNIPKKGDILSWAESSAVVYANSVIGA
                     RCNRNSALIEMFGAILGKVPEFDLLTDEGRKATWVVEVKTSKKPEAQVLGSAIGMKVM
                     SDVPYIKGLDKFIGKELSEQAKDYLKDMGAATASNGAVGLYHVDNLTPEAVELKHALI
                     QENAKVYVIDDAELERVKNSYPIIWKKPDAKPSRCFIGCPHLSYNQLIEWTDRIGREL
                     DKQKKTKVALETIFTSAPDVVAHFKENKEYHERLLSYGVRISSICPLMYMNNPICAKR
                     AVVTNSNKLRTYTTARYYTDEEITSIAVRGDISK"
     gene            84925..85368
                     /locus_tag="PLQ68_00925"
     CDS             84925..85368
                     /locus_tag="PLQ68_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007060438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF126 domain-containing protein"
                     /protein_id="HXK71241.1"
                     /translation="MKKEFKGRNVVAGTVQAQSVVSRKGMNTLATFQKSILAKKKTVF
                     GSDQNNEDLYKKEITGKALCLPKTIGSTTGGMVLQSAAALGLAPKAMLFSESIDSIGA
                     AGVILADVWTVHKIVTVDNLGQDFLDYVKDGMTVTVKEDGTVIVE"
     gene            complement(85572..86894)
                     /gene="melA"
                     /locus_tag="PLQ68_00930"
     CDS             complement(85572..86894)
                     /gene="melA"
                     /locus_tag="PLQ68_00930"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HXK71242.1"
                     /translation="MKKFAFIGGGSFGFTRNLVKDLLTFPAFKDAHIVLMDIDTERLD
                     YIHQAVKKIIKAGNYPAKVTATTNRIEALKDADGVLCTILAGDVDVWQYDILIPKKYG
                     VDINVGDTRGPSGIFRALRTIPVMLDICKDIDRYCPNAVFLNYTNPMAMLCRAMQLKY
                     PHLKITGLCHSVQGTASMLADWIKAPADEITYKCAGVNHQAFYLEYKWNGKDAYPLIK
                     EALKDPEIYKKELVRNNMYEHLDYYVTESSGHNSEYVAWYRKNPETLKKYCMDGTNWN
                     PGHHGYILDEYMKRKDNWRDSIREWLDKDEVDLVRGHEYAIYIFNAVFGDNTMFKFNG
                     NVINYGNIENLPYDCCVEVPVLASKDSLEPIHIGKIPDHLATLINTTARCEVMAVDAA
                     LEGNAKKVFHAVAFDPLTAAVLSLDQIKEMVDEMMEKNKEYLPQFYKK"
     gene            87022..88836
                     /gene="typA"
                     /locus_tag="PLQ68_00935"
     CDS             87022..88836
                     /gene="typA"
                     /locus_tag="PLQ68_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009984398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HXK71243.1"
                     /translation="MIREDLRNIAIIAHVDHGKTTLVDAMLKQSGVFRKNQVVAERVM
                     DSNDLEREKGITILAKNASVRYKNTKINIIDTPGHADFSGEVERVLKMVDGVLLLVDA
                     FEGPMPQTRFVLSKALELNHKVVIVVNKVDREGADIEKTENDVLELLMELQANDMQLD
                     SPYVFCSAKEGVASLSATEKGVDLIPLFDTIISHIDPPKGEEGKSLQVLISSIDYNDY
                     VGRIAIGRVERGTIKANSEVAVVNYHRPKEQYKAKVTNMYGFEGVKREPIDMAKVGDI
                     ISFSGIPDISIGDTICDIENIEAIEFAKISMPTIEMTFSVNDSPFAGREGKFVTSRHL
                     RERLFRETMKDVSLKVKETETAESFIVMGRGEMHLSILVETMKREGYEFQLSTPRVLY
                     TEVDGVTQEPMEHVYIDVSSDSVGSVCEKMGRRKGEMLCMNLTGNRMKMEFIIPARGL
                     FGYRNEFLTDTAGEGIMNTLYEGYAPYKGEIEYRSSGSLIAYETGTAVTYGLYNAQER
                     GILYITSGEEVYTGMVVGETPKDEDITVNVCKKKHVTNMRASGSDEALRLSPPKKMSL
                     EQAMEYIKEDELLEITPINMRIRKKILDPVLRMRSRIK"
     gene            complement(88850..90157)
                     /locus_tag="PLQ68_00940"
     CDS             complement(88850..90157)
                     /locus_tag="PLQ68_00940"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HXK71244.1"
                     /translation="MNKKINILISLIVIILILSSCTPLNTPSVSSPSQISTVTPSDSA
                     TEKPPSQNPTPSAEPSGETPEPPTKTTLKLSAIGDVMSHYTQILDAYNAETGEYDYSN
                     SLSRVKNYTKTTDLLIANLETSMSEGEYTGYPVFNTPPALAKNLKDIGVDIVSLSNNH
                     CLDKGFNGLVSTIENVENAGLEHLGTYATQQQRDENNGVLVKEVNGISMAFVNYSYGT
                     NVIRIPAGKEFSVNLLYEDYLGPYSVLNKTQIEKDMEYAKSLDTDLIIALLHWGSEYV
                     TSENASQREAAQLFFENGADIIIGNHTHVLQPMGFETIQTTDNQEKEVFVCYSLGNFM
                     SNITRTHYTLTSAILNLYIEKDLATNVTTVTGYDYVPLYMLTPEWGGSRFYVLPVHDA
                     INEYEYGSSSIINEKIYNDLIESLKISHSILGKEYDYYHIGRQ"
     gene            complement(90181..>91595)
                     /locus_tag="PLQ68_00945"
     CDS             complement(90181..>91595)
                     /locus_tag="PLQ68_00945"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HXK71245.1"
                     /translation="DYKNSISCGLSYGLWINENGTVSTAGDSSFGKTDVQEWKDIVTV
                     ATGDSFSLGLNYDGKLLYTGFSDSYALNVNHWSSIKQISSGSFHTVAVTSTGSVLSTG
                     SNSAGQCNTSIIAGVSKVECGSMHTVLLRSNGTVRCVGTNYFGECNTSGWTDIVSIAA
                     GNGFTLGLKKDGTVIAVGNNSFRQTQTSDWTEIIEIFAGYNYAIGLRSDGTLVYTGLA
                     TPLSQIIPTLTNIKTISCGRDMIIVKTNEDHISILGNVQAQERTTNKIYVFNYDDISQ
                     DYWAAPYIHIVSRLGIINGVYNGTGYIYIPYVHMTRQEFIKVIIETMGIPVKDLDLNV
                     EVFEDYDDIAQWSLPYIKAAYIYGYLTGSVDSEGKIYLRPKDPISRAEAAAIMGRAFG
                     IGQSSDIDYIDKDTIPYWAYDSISYFSEMKVLSGYPDNSIRPNSELYRSEAAAIICRL
                     YSYVLNGYTSNKVPEPSTEP"
     assembly_gap    91596..91695
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<91696..92756)
                     /locus_tag="PLQ68_00950"
     CDS             complement(<91696..92756)
                     /locus_tag="PLQ68_00950"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HXK71246.1"
                     /translation="MWLLLLVIFIQTLPANALDESFDTRFYWLYSSAIPAGWKVLSDM
                     ETKPVTVAVIDTGIETDNEHLKDRIDLENGRNFVASSAITDFEDKEGHGTAIAGIIAG
                     VPTEDTAYYGVCGNLDIKILPLKVYSDITGALRLQHIIDAIYYAISMNVDVINISIDI
                     PHYDEDLQIACLAAYEKNIPIVSSAGNKGNEYYSYPSSNYGVISVSALSLYNNEYFLS
                     SPFSSYNDRIDICAQGEDIFTLGINGTYTFASGSSFSCAIVTGAVAILKAINKDLTPL
                     EIETILCYSADSLTDAGRNNYYGYGKLNLLSALNTDLNSLSFHSEIQPEERETPFSFE
                     YIDYKNSISCGLSYGLWIN"
     gene            complement(92833..>93153)
                     /locus_tag="PLQ68_00955"
     CDS             complement(92833..>93153)
                     /locus_tag="PLQ68_00955"
                     /inference="COORDINATES: protein motif:HMM:NF027787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5050 domain-containing protein"
                     /protein_id="HXK71247.1"
                     /translation="DRLYYTNINDESYIYSMTFDGKDNKRLENVKGWFLSSNPNDTEN
                     IYFKAANGSLKKVNINTLNQITISSDDCANINILDSYIIYLNQDNDNYYILDTLTGKS
                     HILQ"
     assembly_gap    93154..93253
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<93254..93965)
                     /locus_tag="PLQ68_00960"
     CDS             complement(<93254..93965)
                     /locus_tag="PLQ68_00960"
                     /inference="COORDINATES: protein motif:HMM:NF027787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5050 domain-containing protein"
                     /protein_id="HXK71248.1"
                     /translation="MIKKKFKINLRNKQHRTVIIAAGLAVILITASIVLFSLFNKSYN
                     DIEGNTPSNIINMGIASYEDGNLYYTKFDAGFKIFKQDLKKGNEKSIFSANAKYLNVF
                     QGYVYFIDGNNGTIKKISSSGSEEQTLYESICSHLYVTRDWIYFINNEENNSIYKMSM
                     YDLSVTKLSEGSAASMFVTDDYIFFTNYYDNNSIYRLDLQNNESISILNQHTIFFTVL
                     QDRLYYTNINDESYIYSMT"
     gene            complement(93999..95300)
                     /locus_tag="PLQ68_00965"
     CDS             complement(93999..95300)
                     /locus_tag="PLQ68_00965"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014425376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HXK71249.1"
                     /translation="MRMYDIIQQKRDGLVLSDEQIRYFVKGYTEGSVPDYQAAALLMA
                     IYFHGMNDHETTTLTLAMAESGELIDLSSINGITVDKHSTGGVGDKTSLIIAPCVAAL
                     GYKVAKMSGRGLGHTGGTVDKLESIPGYRTSLDKQEFFDNVNKIGIALVGQSGNLAPA
                     DKKIYALRDVTATVGSLPLIVSSIMSKKIAAGSQNILLDVKVGTGSFTPDLKDAVKLA
                     EKMVEVGKLAGRNIAALITNMDVPLGKAIGNSMEIKEACEVLKNQGPCDLTEVCVELS
                     TYMVWLCTNDSYEKCKSRVVESLKNGTAFNKFRDLVIQQGGDVRYIDNLDLFEKTAFS
                     YDVLALEDGFIYSMNTTAIGEVSLMLGAGREKKEDSIDYSAGIYIHAKTGDKIKRGQV
                     LATLYTNRKGILSEAENEYKKAVKISKSAPNEQKLIYQLVK"
     gene            complement(95303..96262)
                     /locus_tag="PLQ68_00970"
     CDS             complement(95303..96262)
                     /locus_tag="PLQ68_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXK71250.1"
                     /translation="MDMLYLLLRQTMFFSIPLLIVALGGMFSERSGVTNIALEGIMVM
                     GGFTGIYFINQMQSGMSGQLLLLLAVIMAIAGGVVFSLLHAYASVNLKADQVISGTAL
                     NMFAPAFVIYLARMIQPSGIANVSFKNTFQISEVPLLSKIPFIGDVFFKNTYITTFIG
                     FLILIIATVVLYKTRFGMRLRACGEHPQAADSVGINVYKMRYIGVMISGALAGLGGLV
                     LTIPISTEFKGAVNGYGFLALAVLIFGQWKPMRILWAALFFGFMQTISAAYSAIPFLL
                     NLNIPSEFFKMTPYVATLIVLAFSSKASKAPKAVGQPYDKGAR"
     gene            complement(96262..97431)
                     /locus_tag="PLQ68_00975"
     CDS             complement(96262..97431)
                     /locus_tag="PLQ68_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014932546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXK71251.1"
                     /translation="MKNNTSLSEFFDNFRRKKGTANFTSSLIAIGIGLVIGIVILLVS
                     NPAESFAGFFTMLVGGFNDGTRSLGNVFYFATPIIMTGLAVGFAFKTGLFNIGASGQF
                     IVGAYVAVLVGVKLPVLGGFQWVVGILAAMIAGAIWGAIPGILKAFLNVHEVISSIMM
                     NYIGMFLVNYLIKQTVLNVYTNRSLPVAATANIPKMGLTTIFPGSSINGGFLVAVLFV
                     VLIYIVLEKTTFGYELKACGYNRYAAKYAGINETRSIVLSMAIAGMLAGIGGALFYLS
                     GAGVYIEVVDVLAAQGFTGISVALLGMSSPIGILFSGIFIAWLQIGGFYMQRYSFVPE
                     FIQIITAIIIYSSAASLIIRNYLEMKKLKKDEKRNQSGITETVIDSLRNNKGGKS"
     gene            complement(97428..98957)
                     /locus_tag="PLQ68_00980"
     CDS             complement(97428..98957)
                     /locus_tag="PLQ68_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013271626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXK71252.1"
                     /translation="MDYIIEMLNIRKEFPGIVANDDITLQIKKTEIHALLGENGAGKS
                     TLMSVLFGLYRPEAGIIKLRGKEANITDPRTASNLGIGMVHQHFKLVEDFTVLQNIIL
                     GAETVKNGFLKMDEAKKKVIELSNRYNLYIDPDAKISDITVGMQQRVEILKMLYRDNE
                     ILIFDEPTAVLTPQEIDELMGIMKELSKEGKSLIFITHKLEEIKKVADRVTVLRRGKC
                     IGTYNVKDVTKEFLSEKMVGRKVQFTIDKKALKPKETVFEVKNLSMKTKGSTRNTLNK
                     ISFKVRQGEIVCIAGIDGNGQSELVYALTGIQHFDEGQILVNGEDWTKKSIRYRNSNG
                     LSHIPEDRHKYGLVLEYSMAYNLVLQTYFTDRFQKRGFLKRNSINEYAKTLIEKFDIR
                     SGQGPLTTTRSMSGGNQQKVILAREIDKEPSIMIAVQPARGLDVGATEFIHKQLVEQR
                     DSGKAVLLISLELEEVMNLSDRILVIYEGEIVGELDPKKITVQELGLYMAGSKRDEVK
                     A"
BASE COUNT        33046 a        16468 c        16355 g        32837 t
ORIGIN      
        1 tattatacaa cttatggatt tgattgtaaa tctgttttaa tcattacatt gcattgttta
       61 tgtgatattt aataggaaaa ttataataac taattaaatt atcatgttta ttcatatcat
      121 aagtgtcatt cataatatcc tttgtactct tcaatgttaa gagcatattc tccataagta
      181 atatctttgt tattagtaaa tgaaaaataa acctgcattc tttctgataa acaccctttt
      241 atatcattta gtttcattgg cacttcctgc tgttcagtat catcagcagt tttatataaa
      301 acacgaagtg ttaaatcagt aaggtcatca ccttcaaata cgaagcttac attgtcagta
      361 gtcatgtatt cctttgtaat ttctttttct cctgcttctt ctcttgcttc attaaagaag
      421 ataaagaatt cttgccatcc gtctgaatgg atgttattac caagaagcgc tggaaaattc
      481 ataataagag tcccgtctat ttcagaacca ataatcatgt taacaccctt attagaaact
      541 gttgtttcaa aagctgtaaa atattcgcag gaaaacagat agtagttcat ctgccgtatc
      601 tcatatgact gataaagctg ctcgttcctg tcttcaacag gatctcttcc aaccatttca
      661 ttaaaagcgt ttaatcttgc attaagtgtc ttctgtctgc tgatattgtt aaactgagat
      721 accatgggct ttttgatagg gccccctcca tatgtagcaa tgtcgtattc taattcttta
      781 ataacatatc tgtcttctaa ggcattcgca ttattgattg atggtgaaaa cctatctcca
      841 tatggtgtac atgatgttgt gagcagtaat gatagaatta caactactaa aagggataag
      901 cttattaatg ttcttttttt catagtctct tacatgatta tctgttataa atcatgatct
      961 cctttcaaat ttgtttactt cttagccggc tgattactct aattaattgt tcaagatccc
     1021 gctcgtttat atattgaatt ctgtttgaca cttaaattat aaatctcata aaaattttag
     1081 gaaacgctat ttatgtgaca tattatagga aattaataat tcattaagct attttcactg
     1141 ttgtgtatat ctttttaata caaaatatgc aaaaagacat gcgtttttgc atgtctttct
     1201 gcctggcgcg cccgagagag ttcgaatccc caaccttttg atccgtagtc aaacgctcta
     1261 tccaattgag ctacaggcgc acgcaaatgt atattaatac tttttccata tattttcaag
     1321 gtcaatttgc caaaactctt tatgctggct tttctaacct acatatatgc aaattaagac
     1381 tctatcaaaa actgctttta tcgtgatgta ctaattaaca caattatgta ttaggttttt
     1441 aattacagtt tacttaaaca atttttttat atataataat caaacgaagg aaatacctat
     1501 gaaagaaaag tttgacagta aatccatatt gtattcagat acgttaatac ctgacatatt
     1561 catatccgaa tatttgcctg tgttatcctg tgaagctgta aaagtataca ctttctgctg
     1621 ttttttggat aaaacagaaa gaagccttga tatatttaaa atcgtacgaa ggcttgatat
     1681 cgaagaaaat gtactggttt ctgtacttga tgaacttacc aaaaagcatc ttttaacttt
     1741 gagtggaaga gatatcttct taaatgacat aaaaggtatt gagattgaca gactatataa
     1801 agaacgcact tccattaaac cagaggactt aggtgaaaag gaatctgtta tctctgctat
     1861 aaataatcaa ttcttcgatg gcaatatgcc aatatatatg tatggttgca tagagcaatg
     1921 gtttaagaaa tatcgttttg aagatcctgt catgatgatg ctattctcca tcagcaatga
     1981 aaaaggcgca ttaacacaaa actatataga agccgtagcc aaagactggt tcgaaaacgg
     2041 agttaagaca gtatttgatc ttgaagctct ttttactgag cgtgaaaaga tgagggatat
     2101 tcagtcaaaa atacaaaaag ctctaaatag aaagaatccg tttacccaat atgaaacaga
     2161 cataatcaat aaatggttca acgagtatca ttacagtttt gaaattatcg aagaagcatt
     2221 aaagaaaact gtaaagattt caaatcctaa tattgcatat gtagataaaa tactatcttc
     2281 atggtatgag aacgaattta aaaatatcga tgacatagag tcagaaaaag ctattaaaga
     2341 tttaactcca aatgaactta gaatgattgt ccaggaacat taccaaaaga taaatatgag
     2401 aaactccatg cttttcgaaa acaggaaggt tgaagttttc aaaaaagctc ctgctattga
     2461 aactttatat aacgacataa atgatttatt gttcaaacag gcattttctc cagataaaaa
     2521 ggcaattgct gaggaaatta agaataagaa ttatgaaatg actcttcttt tcaaacatca
     2581 taatatacct gatgattatc taaccagaca gtatgattgt gagttatgta aagataccgg
     2641 ggttaataat ggaagagatt gttcctgcaa aatggaattt ctaaaatctt tgtctggcaa
     2701 aaaggaaaaa tagcttttta atctgttaag ttaataatat taatctcttt gtttttaata
     2761 agggcatctt caatatctgt agcaaaatat attatctgtg ttttagtact catttcataa
     2821 agaaaatcga acgtgctctt ttttctttct ttgtcataat aaactaatgc ttcatccata
     2881 aaaacaggag ctactttctt attggaaaac acatttgaag cagctaatct gaatgcaaga
     2941 tacatctgtc tcaaggtgcc ttcagaaaaa ttatttgctg aacgtattct gtctgaatat
     3001 tcagtattta aatccatctg actgcctgtt agcagattat cgtgtttctt tgcagttatg
     3061 cgatataaat aactgctcat tttttcgttc atctttggga ttatttcctc ttttatctgt
     3121 ctggatgctt cttcaatacc taatattgca taatcgattg ctttctgctt attttctaaa
     3181 tgtatagtct gttcttctaa aactgctatt ttctcctgtt gagaggcaat ttgatcgtct
     3241 gatttcagat atttctcaat ctgtgcatta attacggcta atctctctct tttcctttgt
     3301 atctgtctta cgcattcatc atgacttatt tgaggatatg ctactttagg actgaatact
     3361 tctgcttttt ctttaagagc ctcaaaatct gcaacatcct gatttattag tatatctgac
     3421 ataaattttt catatgttct aataagatct tcttttgatt tgtttacttc cagttttgct
     3481 ttagtctttt ccagatttac ttttttgtga tttatttcat ctattcgttc aataatactg
     3541 tgattttctc tgtttttctt tgataacaga aatagtccta ttccacaacc agctgctata
     3601 agagctagaa gaaggaacca catgtctaca attatagata atattatacc tgctgtaaga
     3661 attaaaactc ctataacaat aagaagagta ataataaaat tatttatttt aatattttta
     3721 tacagattat ctttatcttc ttcattaaaa tttacagctt ttatttcttc atcaagaagc
     3781 tctttgcttt tttcatattc ttctatgtca attacagata atcctttttt taatgaatca
     3841 gcttcgcact tatttttttc ataattttta atatctttaa ttgtatcttg atacacctgc
     3901 ttttttacgc acagtttttc ttcatgttcc agttgtttta tttcatcttt taataaacta
     3961 gcttctgaaa gatattcaat gcaattctgg ttttctatct gcatttttac aagttctgct
     4021 ttttctattc ttatattgga aataagattg ttgtatcttc tttttgatgt tctttcatta
     4081 cctaaaaaag tctttccatc cttaagtatt tttatagagt ctgctactga aacatcttct
     4141 tccgctgact cacttatgtt cataagtttt tgagctatat tctctttatc ttcagaataa
     4201 agtatacttt tgtcctgaca tacaaatgca gagttcataa aacactctaa atccatacct
     4261 aataggtcct gtccgagcat accgttttct ttactaaaag agaactcgct ggttatatct
     4321 tcattatttt cgttatatac cgttagagat ttgctgttga aatctctttc aatcctaaga
     4381 attcttccat tatctgtttg tactattaaa taaccgccat atttatcggt attccatggt
     4441 ttgtattttt taacctctgg taatgaaccg tcttttcctt ctctttcatt ttttttatgt
     4501 ccgtaaagca tggatttaac aaacgacata agcgtactct tacctgtttc gttataacct
     4561 ataacagtat tcatgccctg gttgaaattg aatgtcttat ttataaactt accatatcca
     4621 taaatatgtg ctgtaacaat cctcattcta aatccaaacc ttcattcatt agtgccttca
     4681 accctaagtc aagagcatct ttgtatattt ctttttcctc agttgatgct ttcataattc
     4741 tttcattcat taccttttca aaacgtcctt ttattccttg tgaaacggtt tcacagtttg
     4801 ctctttttgg taattctctt ttatctataa tgtgtaagta atcatatcct tcaattggat
     4861 ttacaatatc attttcgttt ttcatacctg taacttttat gcgatatata gtggatttat
     4921 ctgggtattc ctgttcaaat gtgcttaatt tctcataagg tatagttata tcagcatatt
     4981 ttcttgatct gctttcaaca aattcgattg tttttgtttc gaggtctcct ttaaaatatc
     5041 catgttcccc ttcatcatca acctttaact ggcataaact ccccggatta taaatgttat
     5101 ccttgacata cattttatga ttatgtccca tcgctacata atcaaatcca atatccttta
     5161 acaactctga atttacagaa ttatatgtcg aaatacttaa atcggcatct ccatgtaaca
     5221 ttagaacatt agtatatctt ctatctgttt ttattgtttc cagttctcta aactgtccac
     5281 caccggtatc atatcctata ccatatacac atactttttt ttcttcaaaa aacatatatg
     5341 ggtttttcct tcctagtata aatacattgg atgcccatgg atagctgcta tagaaagagt
     5401 tcttggattc tgggtcatga ttcccgcaaa taaggataac tcgtcctttt agtttactga
     5461 aatattcatt tatctctttt atcgttttaa ttttagtata ttcatgttca tacatatcgc
     5521 cacttataaa caagaaatcc ggttgttctt caattgcctg ctttaatata cgtttaaatt
     5581 cagaaaatat ttcctctctt ctttttgaag gaaatccgtc tactgatgct aatgttgaaa
     5641 atggctggtc aagatggatg tcagccaaat gataaaaact tatcattata ctactcctct
     5701 ttaaatgtta ttacagcata tgctaccgaa taatcattgc aatgcgacaa actgatacta
     5761 atttctgtta tatcaggaaa atcttttttc aagtcttctc ctagtactac ataaggcttt
     5821 cctttttcgt cattaagtat ttctatctgt acaggctgga caaaaccaat aaaccctgta
     5881 cccaaagctt tagctaccgc ttccttccct gcaaagcgtg ctgcaaggct ttgtgccata
     5941 actgcctttt ttgattcgca ataactgatt tctttcggtg tgaaattttt caaaagaaac
     6001 ttttctgatc tgtttactgc atcaagtatc cttttattct caactatatc tgttccacag
     6061 cttattctca tgaaaggccg cctgatgttt ttgttatata tatgtcaatg tatttttttg
     6121 ctaattcagc atatgccata ctggcttgtt tcatctcttt gtaaactgct actgtagccg
     6181 agcctgaacc tgtcataaaa gctgaaatcg gttctgtttt atttaaatca tccagagcct
     6241 cctgtataga aggcttatgc ggaattacta cattttgaaa aatatttaca attttaccct
     6301 ttaaaccgac tagatctctc ttatccattg tttcctgtat taaatccatg tccggcctgt
     6361 ctggattctt gcacttgtca tattctctat atgcccatga tgtatcaaca ctttcttttg
     6421 gcttgataat aacaaaccac acatctgaaa aatctttcaa acatgtaagg acttcacctt
     6481 ttccattaac tctttgtgtg cctcctctta tgcaaaaggg aacatcagag cctattttca
     6541 ctgacatttc ttctaattgt tcaaaagaaa gatttgtttt aaaaatatag ttaagccctc
     6601 ttaaaacagc agcggcatca gcgcttcccc ctcctagtcc tgcagatatg ggtattgttt
     6661 tgtataaatc aattttaatg ccttctttaa tgtccatttc tttcatgaaa aggttagcag
     6721 ctataaatgc agtattctcg ttctgtttta ttgaaggggt atcacaccaa aattgtatgc
     6781 ctttttcttt ttcagtaaaa gttatcctgt ctccaagttc aacagtctgc atgatggtat
     6841 caatctcatg gtatctgtcc tctcttagtc ctttgatatc taaaccgata ttaatttttg
     6901 catacgcttt ttgaacaatc atccttaaat ttttaacaca acctttacgg ctttactgtc
     6961 agccatcatt ttaaatcctt catttacatc ttccatggga agttcatgag ttattacatg
     7021 gtcaataaaa gaagcattgc tttttaggaa attctggcac tgataccatg tatcaaacat
     7081 ccttctgcca ttaattcctc ttaatgttat tcccttgaaa ataacatact tacccatatc
     7141 aatattcaag tcttcagaag gaatacccaa tatagccata tctcctccgt tttcaataac
     7201 ctcaagtcca agttggaaag ctgatggaaa accgctcatt tccaacacga cttctggctg
     7261 atgaccatta aatgcttcaa gtatctggcc ttttggatca tctcccaaag gatttataac
     7321 tacatcagcc cctgctttca ttgcaagttc tgccttgtat ggctgtggtt cgcttacaac
     7381 aagtctggca gctccaaatc ttcttgctat tgcggttgca aacaatccga ttgaacctcc
     7441 tcctgttata agaacatccc ttgaggatat aggctgagcc attactgtat gcattgcatt
     7501 tcccaatgga tccattatcg ctccatactt atcttcaatt tcatcatcaa gaggccagac
     7561 attcaatgcc ggcattttta tgtattctgc aaagcatccg tctctatcca ctcctatgat
     7621 ttcaatatca ttgcatatat gtgctctgcc agttctgcat aacttgcact tctgacaggt
     7681 tatatgacct tctgccgata ccctttgtcc gatatgaaaa ccttttacgg cttttcccat
     7741 gtctgctatc tcacctgcaa actcatgtcc cataatcaaa ggaggtttta ttctgttctg
     7801 agaccactta tcccacttat aaacatgaaa atcggttccg catatgccgg ctttctttac
     7861 ttttatcaaa acttcatttt cgttaatttc cggaacaggt ctttgtacta gctgtacatt
     7921 tcccattcct gcttcagcct ttataatcgc tttcataaac tctccttaac agcatcttga
     7981 ttttatcata ttagaaggta aatgttaatt gatttgactc atttgggaaa cattttcgta
     8041 tttagcacgt catataaagg aagatataaa tgaaggagta cgatatgaaa aaaatactta
     8101 ctttaataat cgctataatt acaatatcaa ttcttgcagg ttgcagtaca tataattata
     8161 ttaactcata tcctgtaaat atagatgacc tgactgttga gtatatacgt actaatggtt
     8221 attatgaaga tattgagtat cctcttgtct ttgttattaa agacaaagac cagcttgaat
     8281 catattttaa tgaaaagaaa gacaaataca atatggaatc tgacggtgag gatagtttta
     8341 gcagcaaaat gtataaatat gacagttcct tttttgaatc aaatatactg atagctttac
     8401 tgattgaaga acctagcggg tctataaggc atgagctgac cttggtagaa gaagaagata
     8461 ataagataac attcggtata actcgtgatg tacctgaaat tggaacaaca gatatggctc
     8521 aatggcatat acttataagc atgccttcgc atttatacaa taatcaagat atcgatgtgg
     8581 aatttaatta atttaattta tgctgtctct ttgctcttgt ttttattgat gtacatcatt
     8641 ttatccattt ttctgaagaa atcggtaact gtctcgtttg aaacatggat tccatagcca
     8701 acacttgccg ttattttaaa aggaagctct ttcgttgagt taaacttttc aaatccataa
     8761 tttattttat caatatactc atatggttcc tcttcatcgc tgacttctat tacaacaaca
     8821 aactcatctc cgccatatct tgcaataaaa tctttttcac taaaggtttc tcgtaatatc
     8881 tgagcagtat attttagtgc aagatcacca ttatcatggc catataaatc attaattttc
     8941 ttgaatgaat ctatatcaat cattaatcct gctatttttt tattgctact gttaattttt
     9001 gctcttatgt aattatcaaa ctgtcttctg ttatataaat ctgtaagata gtccttatac
     9061 aactggccgt tttgaatatt tatgtagatg ataagcaatg aaagagtcgc acatatccac
     9121 aatagtgtta ttccataaac taaaatctgt ataacaccac ctataactgc tggtactatg
     9181 aataatgcaa gcgacaggaa ttccctctta ttgatcattt ttcgtttaac aattagtaag
     9241 atatatgcat atgctatgta taagaaacat ataagaacca ttacaagaaa atatctgcct
     9301 ctgtgatata tgttatctgc atctatgtaa tatatgacat cagcaaatag acttataatt
     9361 gccattatag caactgcaaa aaaaggaaca ccaatagtaa atatttgttt tttaagagca
     9421 accttgtttc tgtatataaa atagtgaaca taaaggtacc agaacaaaca tatcaatgta
     9481 ttaaatatgt agtacagaaa agtagaaata tacataaaaa tccttgcata ctgacctggt
     9541 actccgtcaa gcaaaaccat tgctgagtca agcactagta aaaatatatt gcttatgacc
     9601 atagtgataa aaagtttctg atcaagaaga agtctgcttt ttttacttct tataaaaata
     9661 agaagcacta acataattga tacagcaaat aagttaagtt caatataaag ggtggatgca
     9721 aaagctgctc tatccatcta tggccttcca atgtagtaat aaatacatta tagtacaaaa
     9781 tatcaatatt aaccacgtta tgtttataat atcggctttt ttagcttttt gtttagtttt
     9841 ttgttgttct gccttattta taaaaccctt ttattacttt tatagtataa tattacttat
     9901 gaaagattta tttgaaaagc ttatgcagcg caggcttttt agggaattgt tccttttatt
     9961 taaatacggg cttgtaggca tcattaatac ctgcattacg acaggtattt ttttgctctt
    10021 aagttactta ggccttgaat atgttctcta cacaataatc ggttatgctg ttggcatttg
    10081 tataagtttt ttactcaaca gaaagtacac tttcaaaaaa gagaatgaga atgcaacacg
    10141 tcagattata ctttttttca caataagtgt atcattgtta gccattgttc agcttataca
    10201 atacctgctt atagacatat acaaccttca agagtggctt ggtgtaggga taggaatggt
    10261 gttttacact ggtacaggct ttattcttaa cagaaatatt gttttcaaat atacagataa
    10321 gccggaggaa taaattttga cagacagaat tataaaacag atagaattta taaaagaaat
    10381 agataagatg aaaaatatct taagaatgac tcttgttagc gaatctgaaa gaagagaaaa
    10441 tgatgctgag cattcatggc atcttgctat gatggcgatg cttttagaag agtattcaga
    10501 taagcctgta gatatttctc atgttctaaa gatgactttg gtacatgatc ttgttgaaat
    10561 atatgcaggt gacacttttg catatgacat ggaggggaat aaggacaaag aagaaaggga
    10621 aaacagagca gctgaaaaac ttttctcgat gcttccggac gaccagaagg atttaataat
    10681 gtctttatgg aaagaatttg atgctatgga aactgatgat tctctttttg ctaacgctat
    10741 ggacaggttt cagccgtttt taagtaatgt tatgacatcg ggacatactt ggaagattgg
    10801 aaaagtcacc aaagaaaaaa ttctttcaag aatggacccg ataagaatcg ctactcctgc
    10861 attgtggcca tatattttag atactgtaga acaattacat caaaaaggat atatctataa
    10921 ataactattt aatctttttt accaaataac ctgcttcttt taccgctttt gtaattgctt
    10981 catcagattg ttcagtttca acaaatgctg ttttcttttt taaatcaatt ttcgcatgtg
    11041 tattttctaa acggttcaaa gcatcttcaa ctctttttga acaatgagaa cagctcattc
    11101 cctcaattgt aatttccttt ttcatttttg ctcctttttc attatttggt ttaaatcttt
    11161 ttaatctaag agcattagca acaacactta ttgaactcat gctcattgca gcagctgcaa
    11221 tcattgggct caatgtccac ccgaaaacat tataaaagac acctgcagct ataggtatac
    11281 ttattgtgtt atacaaaaag gcccaaaaaa gattctgttt aatgtttgta aatactttct
    11341 tgcttagttt tattgatgtt actacatcca taaggctgct tttcataagt attacatctg
    11401 cagattctat tgctatatca gttcctgctc ctatagctat tccaacatca cttgcaatta
    11461 atgcaggtgc atcatttatg ccgtcaccta ccattgcaac tatttttcct ttttctctaa
    11521 gagcagatac cttctgctcc ttttgtacag gtaaaacgcc tgcaattaca tcatctactc
    11581 ccgctatatc agccattgct cttgctgacc gctcgttatc tcctgttaac attattacat
    11641 ttatacctag cttctttatg gcttttattg cttttatact ttcaggctta agaacatcag
    11701 caattgcaat aattcccatt atttcatcat ttcttgcaat gtatacagga gtatatcctt
    11761 tatcagcata atcattgtaa atctcagaaa cactttgcac atttatattg ctattctcca
    11821 taagtcttct gtttccgact ttatatatat taggaccaat acttccaagc actccttctc
    11881 cctgaagcgg ttggaaatcc tttacttcct ctagacttaa gcccagttct ttatttttgg
    11941 ttaatatcgc atctgcaagg ggatgttcag acatcttttc caaagatgct gccattgata
    12001 acagatgatt ttcttcacct attactgcat gaactgcaac aactgttggt tttccttctg
    12061 taatcgttcc tgttttatct aaaacgacag tatctacttt atgcagtatt tccaaagctt
    12121 ctcctgtttt tatcaatata ttatgctcag cagcttttcc tgttccaact ataactgata
    12181 taggtgttgc taaacccaaa gcacaaggac aggatattac aagaacagat atagctatag
    12241 aaaatgcaaa atcaaaacct ttacctgcta aaagccatat tacaaatgat gatagagcaa
    12301 tcaatgtaac aacaggcaca aaccatctgc ttatctcatc tgctagtttg gcaattggag
    12361 cttttttcat tgaggaatct tcaacaagtc tgactatctt tgcaactgtt gtattttcac
    12421 caacctgttc tgctttgaat ataaagtatc ccgatttaca tatgcatcct gatattactt
    12481 tatccccttc cagtttttct tcaggtatgg gttcaccagt cacactggac tggtctacat
    12541 agccctggcc ttttacaact gttccatcac atggaatcga gtctccctgt cttataataa
    12601 ccagatcatc taactgtata tcttcgctta agatttcaat ctcctggtca tctttcaaaa
    12661 taactgagac ttttggagtt aaatccataa gctttcttaa agcttcacca gttttctttt
    12721 tcttttttgt ttcaaaatac ttccccaatg ttaccagtgt tagaatcatt gccgctgatt
    12781 caaaatatat gtcatgaaca taatgagcat taggattctt ttctataata actcttacaa
    12841 aaatatataa cccgtaaatt atagcagctc ctgctcctgc tgctactaat gaatccatat
    12901 ttggccttag ttttattatc tgctgaaatc cttttataaa gtatgacctg ttaagtattg
    12961 caattattgc tgtcagaacc aaaagaatgg aaaaaaacag tactatatct gtcgtgaaaa
    13021 tttttggtat tggaatattt atcataggcc ccattgataa atacatcaaa attacgagta
    13081 ttactacaga tgagataatc cttattctca tctttttttc ttcttctata tactcgtcac
    13141 cttttttaat tccatatcct tcttttttta cagctttttc tatatctgct tctgtaactg
    13201 atgtttcatc atatacgact tttagtgttc cgttggaaag attgacgcct gcctgcttga
    13261 cgcctttaag ttcttttact gcagtttcca catgattgac gcagactgag catgtcatcc
    13321 ctgttatata aaatttcttt tctgccatgc taacctctct atctgttaat ctaagttatt
    13381 gattcccatc ccctgaattc attagggaat gttattttta tggtagttcc cttaccaagt
    13441 tcgctttcaa cttcaattac gccctgatgc agatccacaa tcctctttac aagagctaaa
    13501 cctagtcctg ctccctcttt tgaatgagag gaatctcctt taaagaattt ttcaaacatc
    13561 ctgtctttag tatgcttgtc cattcccatt ccttcatctt gtatttctat aactgctttt
    13621 tcttcttctt tgtacttata cattcttata tgaattgtac cattttgata tgaatatact
    13681 attgcattat ttaaaaggtt aatccatacc tgctgcataa gatcttcatc tgccaagaat
    13741 gatatatttt ccatatcaat atcaaaatct atattttttt cacaccattt aggttcaagc
    13801 agtaatatta ctcttcttat ctgctcatct agactatatt ctgctttgtt gaacactata
    13861 tcctgacttt caagctttga taacttcatt atatttgtag aaagattgga aagcctttcc
    13921 gattcttcaa caattatttc gagatattcc ttcttttcct ggtcagtaag attctcgttt
    13981 tccaacaatt ttgcaaatcc caatatagaa gatattggtg ttttgaattc atgtgataca
    14041 ttggatacaa aatcttttct taagtattca atgcttttca gttcctttgc cattttgtta
    14101 aaactttcag taagctgtcc tatttcatcc tgagaatcat tgaaaacagc aacatcaaaa
    14161 tttcctttag ctacttcctg tgttgcatta ttcaactgaa taataggttt cattgcctta
    14221 ttgcttatac ccattattat aagcataaaa actatgattg aaaaaagaag cgcatatgct
    14281 aaaagcattg cataatttct aaatagatta ttctcaggct gatatcctac acgaagataa
    14341 aactcgtttg ttttaataac agatattcca acaggtatta ccttctgttt aatgaagaca
    14401 atacctcctt cctccaaaat gtttttatct ttatcattaa gatttaaatc ttctatatcc
    14461 tttgatataa ctacaggata tagggcatta tatgttattg ctataattct ttctaatgga
    14521 agatcagttt cctcatttgc ggcaattatt gtttctgcaa catatctctg gttttctttt
    14581 atgttgtcaa taaaacttct gttagatgtc agaattgccc ccatgtatgc aaacatggaa
    14641 gctattatta ttattattaa cagaaatatt gataatttag acttcagttg catttttctt
    14701 ttccgcctta tatcccaatc cccttacagt taatatctca aattctttaa tatcagataa
    14761 tttttctctc agtcttttta tgtgtacatc aacagttctt tcatctgtct ctgtgtcaag
    14821 tccccagatt tcatccatca actgctgtct tgtaaatatc ttttttgggt aactcatgag
    14881 tttgaataac agcataaact cctttttagg aagtatgatt gtttctttcg aactctgaac
    14941 acttaatgta tcataatcta aaaccacatc acctgcttca agtctgtgct ctgttgaaat
    15001 ttttgatctt cttaataatg catttatacg tagcagcatt tcatctatat taattggttt
    15061 aaccatatag tcatctgttc ctgatatgaa tccttttctt ttgtcttcca ttgtatcctt
    15121 tgcagtaatc ataaggatgg gcatattgta tcctgcatct cttagactgc ttgttaattc
    15181 ataaccgtcc atatttggca tcattatgtc acatatgatc aagtcagctt ttgtgctttc
    15241 aagaatttca agcgcctgtt cgccgtcatt tgcactgaac acattaaacc cgttttgttt
    15301 cagtacggtc cctataagct ttcttaaatt tttatcgtcc tcaactataa gtatgttaaa
    15361 cataaaatca ccttctaatt tgtattatat cattaaatat gaatctgtta tgaattgaag
    15421 ggtttattca actgttacac ttcttaatcc cgcaatttga tcaactggaa tggaaaatgc
    15481 cactgcatga gcctccgtat cagtaccagc cctttccata atggtgccca ttatattctc
    15541 ccgattttcc ttcttgcata gtatatatat catttccttt tcactggcta tagaaacacc
    15601 gaaaaatctt tcctttatat cttcccctgt tcctttcgcc tgaactactg taccacctct
    15661 tgcatttgcg cttcttgctg cttccattac cagatcagaa gatcctctgt ttgctataac
    15721 aataatcaat gaatacttta tatcgtccat tttttcacct tctcctttaa tattctgtcc
    15781 ttttgcgaga tatttcaaac atgattctcc acctatactc tcaagaggaa ttgtaaaagc
    15841 tataccattt cccggtactt caatttccat ctgatttata aggcctttca ttatctcatt
    15901 tctgtgcttg cagtcagcta tagcttggaa cattattttt tctgtttttt ctattcctaa
    15961 atagtcaagt gttttctttt gagcagttcc tttgcaaaac tctgacaaga ccatgaatat
    16021 ctcattttcc ttaaaaaatt catgataagc ttcttcatat tctctttttg tgattactat
    16081 catgtaaaac atgtttccca tcttatttgt cctcattcaa aatttataat tttttctctc
    16141 ttccttgctc tggctttctc ttgtttctct gttgatttta ctttaactct gtaataaaca
    16201 cccagagctt gtattgttat caatggagtc attgcaacca ttgcaacaag gccaaatgca
    16261 tctactacca cattaccgcc tactcctaaa cataccccta tagctaaagg cagtagaaat
    16321 gttgctgtca tcggacctga tgctactcct cctgagtcaa aagctattgc agtaaatact
    16381 tttggcacaa aaaaggtaag tgctagtgat atagcatatc caggtactaa aaggtacatt
    16441 aaaggcaacc ctgttagtat ccttaccata gataaaccta cagaaacgga tactcctata
    16501 gacagacttg ttgttattgc ctttttggga atggctccgg aagtcatctc tactacctgt
    16561 ttattcaata catggacagc tggttcagcg ataacaataa aataacctat aaccatacca
    16621 attggaacaa taatccagtt gttcgcaaga gagcctataa tctgacctaa ataactgcct
    16681 aaaggcatga atccgacatt tactcctgtt aaaaacagaa caagtccaat ataggtatat
    16741 ataagcccaa aaacaattct tattatctgt ccttttttta actttaaaac aattatctga
    16801 aataatccga agaaaacaat aatgggtatt aatgctatag cgacttccat tattctgtgt
    16861 gggaaatccg aaccgaaaag cattgttatc ttttgtgtgt ctgcagcatc aggcagaggt
    16921 ttgtattcat aattacttgc tatccctttg taaattacac caagtattaa aactgctaat
    16981 atcggtccta ccgaacataa tccgactaat ccaaaactgt cactttgagc atccttgtcg
    17041 cttcttattg aagatacgcc tacaccgagt gctaatatga aaggaacagt cataggaccg
    17101 gttgttaccc ctccagaatc aaatgcaact gcaagaaatt ccttaggtat aaaaaaggct
    17161 agaataaaaa ccgcaatata tagtcctaca agcagatatg aaagtcttat cttaaatata
    17221 atcctaagca tagctaacaa aagaaacaaa ccaactccaa cggccacagt cactactaaa
    17281 accataccag gtattccagg tacctgttca gctaataccg taaggtcagg ttcagatatt
    17341 gttattgcag tacctacaat aaaagcaact aagagtataa gccaaatctt ccttgatctg
    17401 gtcatggcag aaccaacatg ctcccctatg ggcatcatgg caatatcagc accaagggta
    17461 aacagaccca ttccaagtat gagcataact gcaccaatta tgaaagtaag catcatgccg
    17521 ctttttatgg ggaaaaatat aaaagaaaga ataaaaacta ttattgttac aggaagtact
    17581 gatgaaagag attctttcag tttttccagc aatatagtcc tattgtgttt aataaattcc
    17641 ctgttttctt ttgacatgta ttatcctttt gtattattaa taataatata tcatatagga
    17701 acatttaatg taataggtaa ctggataaac aataatgtaa cgcctgtcta actttaaatt
    17761 ttccagatat tttatttttt accagtttcc tttcatactt aactttatag ttgcgtgaaa
    17821 tgcattgttt ttgttagaat aatcacataa gacgaaatat caggaggaga tttaataaga
    17881 cgcatatacg gaatattaaa attcacaaca cttttcactc ttgtctggat tgcactaaat
    17941 gaagagctta gtatttttaa tgttttagca ggtcttatct tatctgtcat tactatttta
    18001 tttacaggta gtttattctt caataaagga tatatttcca catatcagat tcctttttac
    18061 agaatgattg tttatcatct ttatgttgtg ttcttaattt ttaagtcagg tatgtctgct
    18121 ataccaagaa ttatagcagg taatgataaa acaaaaacca ttaaatacct ttctggttgc
    18181 aaagatgact ttgcattaag tcttctgtca aatgcaatca ctcttactcc gggtactgtc
    18241 actttggatt taacaaaaaa tgaattgact gtattgtgtt ttactgattc aaagaataat
    18301 gccttgtttg atatagagaa tgcaagaaaa atagaaaaaa tactggaagg gagatgagat
    18361 ggctttttat aacattgcct taatactatt agcaataatg ttcatactgg taattatacg
    18421 ctctgtaata ggtcctacta tatgggacag attattaggc ttgagtatgc tttctgcaaa
    18481 aataattact tttatatgtc tgtttgcttt agtgtcaggt aaaacaatgt ttttagatgt
    18541 tgcgttaatc ttttctcttt taggatttat aagtaatgtc ctgatatccc gatatgtaag
    18601 gaggaaaagc aatagtgatt agaactataa tttcttcaat aataatatca gctggtcttt
    18661 tcttcatgac tttcggaata ataggagttt acagattcag taatttttat gcacgtattc
    18721 taattactgc caaagttgaa acagtaggat ttttagcggt tatgattggt attcttgttc
    18781 aatctggttt cagttatttc tccttcaaaa tagtattaat tactcttttc tttatgttga
    18841 ccaatccttt gtctacacat gcaataggaa gatctgcttt ttacagcaat ctttcagaaa
    18901 aggagatgga taatgattga aaagataatt ctacttttgc ttctggcatt agcaataata
    18961 actattatct gtaaacagta caaaagaaca gttatctttc taggtatttt ctcattatta
    19021 agcgctcttc tttatgttat ttattcagca cctgatgtag caatagcaga agctgttatt
    19081 ggatgcggtt tatcaactgt tttttatctt gttgcaatca aaaaagctga gatattcaaa
    19141 gtatgtatca tatctgaaaa ccagtcttcg aatgaaagta ctgatctcaa taatttatta
    19201 ggaggtttaa aaaaggatat tgaaaataga aatcttttat gtgaaataaa cagcactgtt
    19261 aaatctgtaa aagaagccat ggcttctgag tctgatctta taattcatga gcaagtagac
    19321 agaataacta tttatggatt agaaaatgac catatctttg aaggactgtg tgatgatatt
    19381 gacaaaaact gtgtaatcaa taaaaaaata ttaatcacaa aagtttctga tgttaaacga
    19441 aacaaggaga cggtaaatga gtaaatttaa gacaatagct gttatcattt taatgcttgc
    19501 tctgattata actattttta tcctgtctga cttcagtgaa agtaatacct ctactgcaga
    19561 ttattatatt gaaaacagcg aaaaagatac tgcagctatt aatgctgtaa cagcaatata
    19621 ccttaactac agagtttatg acagtatttt cgagacatta atattattag taagcgtatc
    19681 tgctgtaata catttttctt ggaggaaaga tcatgagtga taaatcttat cttatgtcac
    19741 atttcatagg ctttatgtat ccgattatcc ttcttattgg tgtttacatt atcgtaaatg
    19801 gtcatcagtc accaggtgga gggttccatg gaggtgctgt tcttgctact gttttcatat
    19861 gcagatatct tgaaaatccg aatgaagatt tgagaataca ctcccttgaa atacttgaga
    19921 aactgttatt ctttcttatt cttcttttac ctgttgtttt gctattcaat aatatctcca
    19981 tgagtattat ttcgaacaag ctgtatcttt taataatgaa tatactaata ggtttaaaag
    20041 tatgtacggg cttgtcaata atattttata gattcgtgtt ttatgaaggt ggtggcatat
    20101 ggaactgatt actggtgaga atataagcat attgatattt tttgcaggct tttctggtct
    20161 tatgttaaaa agaaatgtaa ttaagtcaat tatctgcatt ggtattatgc aggctgctat
    20221 aatcattttc ttcttgtctt caggtctctc acctgacaat gtgccaccta ttgatgccac
    20281 aggtttgcag aatgtagcag acccgttacc tcaggcatta atgataactg acatagtcat
    20341 aggtattggc gttacagcaa tggctttatc tttgtttatt catatgtatc atcaatttgg
    20401 aacaagtgat tgggaaaaag cattcaaatt gagaaaggac tccgaagaat gaacaatatt
    20461 ttagtatatc tagttctttt tccaatactt acaggttctt ttctaggatt attgaaatgt
    20521 aacaataccc gtaagcgtaa aattcttaca atagtaacaa tatttataca gtctttatta
    20581 ttaatcggtt ctttatatgt gcttacttat gttaaggatt ttggaacaac cgctcagaat
    20641 ataggtaact ggcctgactt tattggaatt acacttaaag ctgatatata tgctgtttca
    20701 tttgtactga tgagtatatc tctatttacc tttgcaataa tatatcaact tacagaaaat
    20761 gatattgaat caagagtttt ttctttactc ataattcttg aaggattaat aattggtatt
    20821 ttcctatcaa atgatctttt taatatattt gttcttattg aggtggcaac tatagttatt
    20881 agccttttaa taatgtataa aaaagatact atttcgacat atgatggcat gctttatctt
    20941 cttgtaaata tagcagctat gagtcttttt ctttttggta taggtatgtt gtataaaatg
    21001 acaggagtta tagacctgac aggtcttaag gatgttttat ctgtatttcc tgacaaaaag
    21061 gccttaatag ttccatatgg tttgatgctt actgctgttt gtttaaaatc cgctgttctt
    21121 cctttattca gctggcttcc acatgcacat gctactccca gtgctccttc ggcaatatca
    21181 gctgttttat caggtatata tgtaaaaagc ggcatatacc ttttcttacg ccttcagaat
    21241 gtattctctg acgttttcga tacctattat ctcttcttaa tacttgggtt tgttactgca
    21301 gtggcgggtt tcatgctggc tgtttcacaa aaagatatta aacttattct tgcatatcac
    21361 acaatttcac aaatcggaat gataatgatg ggtttgagta tgaacagtga gtcagcatac
    21421 ttaggcgctg tatatcacat tataaaccat gcatttttta aatcctgtct ttttctgtct
    21481 gcagggttga ttatttctgc atataaaagc aggaatgtat ataaaataaa aggggtattt
    21541 aaaaatatgc ctataactgc aacagcctgt gtaatggcaa ttctcggtat aacaggagcc
    21601 cctttgttta atggaagtat ttccaagtac tggatatcat atggtgcaaa gggcggatgg
    21661 gcggagtatg ccttaattct aattaatata ggaacaatga tttccttcat taaatactct
    21721 ggaatgctta ctggtaatta tgatagtact ttagtaaaaa tatcgaaaaa gcgaagcgtt
    21781 cctgtcttaa ttttaagtgt aatgtgcctt ttgggaggaa tcttaagtga acctataata
    21841 tctttcctat tcaatgtaca aatgagcccc aatctgcttg attatcttga aaaaagtctt
    21901 ctgttttttg tatcattagg tattgcgtac cttctatatc gctatgctat atcaaaagtt
    21961 aatttataca aaattgcttc ccgtttggaa cttggtttta acggaatctg tataagtatg
    22021 ggcgtatttt ttctggtaat gcttttatac attcgcatat ttgcaatgta aatagtttga
    22081 cttttatatg ttatttatat atatttcata taattgattt ttaaggagca ttatgaataa
    22141 agtaattttt tgggattttc acaacacact tgcatataaa cataattttt ttatagattg
    22201 cataatcata gttttagata aatatcaaaa ggggcacaca gtgactcatg agtctcttta
    22261 tccacatatg cagactggtt ttccatggct ggaaccagat aaagaatacc atcacttaaa
    22321 aaatcctcaa aaatggtggg ataacttata tccggttttc ataaatgctc taacatcaaa
    22381 tggaatagat aaagacaaag cattacttta tacaaaacag gctcataata ttatgaccgg
    22441 tcctgattac tatactctgt ttgatgatac tttggaaaca cttaaaagag caaaaatcaa
    22501 tggatataga aatattatat tatccaacca tattcctgag ttgccgcaaa tggctaaaca
    22561 gcttggcgtt atggattata ttgatatctg catatcttca gcaaacatcg gatatgaaaa
    22621 accaaacaga aaaatttatg aatatgccgt agaagctgca gataatccag acactgtgtg
    22681 gatggtagga gataattatg aagctgatta tttaggcgct acctccgcgg gcataaaaac
    22741 tgtattagtt agaaacccgt tagatgaagt taatgatgta agcaaaacag aaaaagaaac
    22801 taaaaaagtt gaatattact ctgatgatat aaaaggtgct ttggatataa tacttcaaca
    22861 ataactctat caattgaatg tatttaatta atcaacaaca aatctcaatg tttctacagc
    22921 tgcagcacct tctttgaaat cctcgctttt tgcatgtgta ttgtaaaatg ctactttaag
    22981 ttcaaatatg ctttcataac ttgcgtcacc tatgaaacat agaatattcg ttccatctcc
    23041 tcctgcatat ccgtatcctc ctataagact gttgtctatg tagtagaaag ttccatttac
    23101 gcctctgtgt aatatcttat cctttcccgt atattcagca aatactataa acgaaggatg
    23161 ataagtatca tattctgact ggctaataaa atatgtaaat ctgatatcat ttattttttc
    23221 ttcatattta tcataccctt tattttctga tatagaataa atttctgagt attttttttc
    23281 attatcctct ttatctaaat cgatttctgt ataactgcaa ataggaggtt ctactctatt
    23341 ctcttcattt ctgctaataa ttgcggatac actgattatt attccataaa taattattac
    23401 tgtaagtata gctgccaata taaaacttgg aagagacgga atatttcttt tagtattatt
    23461 aggatcattt aaccctttgt taattagctt cttgtaaatc aagtaatatg cagctataag
    23521 taatgataca aacactacta gagcaaaaaa taaaattata actgatgcaa ttgacctcat
    23581 ttttcctcct ctatatacct agcttatctt tgaatatgta ataatagctt cttgtaacat
    23641 ctactatatt tccatttttc aatttaagca tgaacttctg tgacaatgct ggtttaatac
    23701 tttttatcgc tttctttgat actattactg agttgcttat acgtataaac ttatcaggat
    23761 caagtaagtt ctctaactgc caaagtcttt ctttgattct gtatattgct tcacttgtat
    23821 gtacaagcac gtcatgagca agactttcaa catatatgac atcttctaca tttactctgt
    23881 ggagtatatc ttcaagcttt ccgttaagat gattgacata ggcattgtct tctgaaaatg
    23941 taaattctga gtcatcatct atatcaatac ccaaagcttt aagctctttc tcaatttctt
    24001 catatctttc cttactagct aataatttta ctttcaatat tacccccacc ttgtaataaa
    24061 agcattttta cattaaaccc ttcaattatc aataattttt gaataactta cacatttgtg
    24121 cacataactt gcatactcta tcttttatac ttatatttat atttttatcg ttaatcaaac
    24181 agttctttta taatctttgg aaacagaacc cgtctggtat gccttctgta atccctttcc
    24241 gctttagtcc tgccggttat taatgatttt cttgtaacag tattatctga aacactgaaa
    24301 acagcagtta ttggtatatt tgctagtttt gctgccttaa atgcagcagc tgtttccatt
    24361 tctatcacat tacatccaat ttctataatt tcatcaaggt gataaaactg agcaaatatc
    24421 gtatctatac tgaatgtctt tgctatatgc cattttacat tatgctcttt acataaattt
    24481 tcagctattt ctttgctctt attaaacatt gactcatcag gatattcctt tttcccgaag
    24541 atatcccctt ttgttaaatt atccgaatca atatatctgc ttgcgccgtc accacatact
    24601 gagtattcgg gtataactat atcccctata caaatatcac tatccaaagc ccctacagaa
    24661 cctataaata ctattttttc acatgaagtc atcccaagcg ataaaacaat atccataagt
    24721 acagatgcac cgatgcctgt ttttatatat gtgaattcac aatcatcatt acatatattc
    24781 catactttta tgtgatcaga aattgactta cttatatatt ccgctgtttg aaagtctgga
    24841 aataatgaag gttcccacca tggagcaatt accacattct tatgtatatg ctcttctttt
    24901 acaccaatac aatttatgca tatatctttt gttgaaatgc catacttttc cataccgaga
    24961 attagttttt ggtaatccat gcttttatca tcctttacta ttattaagta gttattgtat
    25021 gttattcatt cattttacac tatctattga cctatttcat aattttctgt ataatcccaa
    25081 cgacattgaa taggttttat tgataattta aaaagtttaa aacaggagac aagacaatta
    25141 cagatatatc aaatacaatt aataatgagg taacacgcag aagaactttt gcaataatat
    25201 ctcatccgga tgctggaaaa accacactaa cagaaaaact tcttctttac ggaggagcaa
    25261 tacgccttgc tggagctgtc aagggaagaa aagctagcaa acatgctgtt tctgactgga
    25321 tggagattga aaaacaaaga ggtatctccg tcacttccag tgtaatgttt tttgattaca
    25381 acggataccg cataaatata cttgacacac ctggccatca ggatttttct gaagacacct
    25441 acagaacact gacagctgct gattgcgctg ttatgcttat tgacggagct aaaggagtgg
    25501 aagcacagac aagaaaactc tttgcagtat gcagactgcg acatattcca atatttacat
    25561 ttgtcaacaa acttgatcgt tactgcaaaa gcccctttga acttatggac gagatagaaa
    25621 aagtattagg tataagagca tatcccatga actggcctgt aggcgaagat ggcagcaatt
    25681 taggagttta taaccgtaaa aacaaaaata ttgatttatt tgaggcaaaa gaacatggtc
    25741 agcgtgccct ttcctctaaa ttaatacctt tatctgaccc tcagacaaag gatatattag
    25801 gtgcaaggat atataacaaa ctgtgtgaag atatagagct tttagatatg gctggagatg
    25861 attttgattt tgagaaagtt aaaactggag aactcacgcc aatgtttttt ggaagtgcaa
    25921 tgactaattt cggagtaaag ccttttttaa aggaatttat tgaattagct ccttcaccaa
    25981 aaaataaaaa tactgagtct ttaaatgaat taccggataa taataatttc agtggatttg
    26041 tatttaagat acaggcaaat atggatcctg cacatagaaa caggatagca ttctttcgca
    26101 tatgctcggg aaaatttgaa aaaggaatgg aagtcattca cgcaagaaca ggtaaaacca
    26161 ttcgtcttgc acagcctcag caattcttcg ctcaggaaag aaccgttgcc gaagaaggat
    26221 ttgcaggaga tataataggt gttgtcgata atgggttact aaatattggt gatgctcttt
    26281 atacaggtaa aacacctttt gtttttgatg gtattccaat attcccagca gagcattttg
    26341 cacaggtcag aacacccgat gctatgaaga ggaaacaatt tttaaaagga atcaatcaga
    26401 tatctgaaga gggttccatt cagatattca aacaacccaa tattggagtt gagacactga
    26461 tagttggtgt agtaggtgta cttcaattcg aagtacttga atacagaatg aaaaatgaat
    26521 atggagtaga tatagtaata gaaaaacttc catataattt agctagatgg gtaatgacta
    26581 agactgtaac aagagaaatg ctcggttttg cagacagcgc caaagctgtt gaagacagat
    26641 taggaagacc ggtaatactt tataacaatg aatggacact taacagggct caggaaaaga
    26701 ataaagatgt tgaatttctt gagattcctc cgataataaa tcaataatgc caactatata
    26761 tgctaagcca ttctggataa atcctctaga tttatctctg ttttcatttt ttcacctgtg
    26821 ttaagtcttt ccatctcttc acatatatga agggctattc ttttacatcc gttatttaca
    26881 gctcctgcta tgtgaggagt cattattaca ttttccatat atcttaattc attattaaca
    26941 tccggaggtt caggatttgt aacatcaatg catgcaaaaa atctgccttt tttcagttct
    27001 tctattaaat ctatctcgtt tacaacagtt cctctggctg tatttattaa cactgctcca
    27061 tcttttataa gacataggtt atctttattc agcatattat ccgtagctgg aattgatggt
    27121 gcatgaatgc ttataacatc agaatcacac aaaagttttt caagctcaac cttctttgct
    27181 cctaaatttt caatatactc tttgctcagt gtagggtcat acattaaaat atcaacatga
    27241 aagtttttca gtaatttcac catatgacgt cctacaaatc ctccgcttat aactcccacc
    27301 gtaatatcat aaaaatcctt aacaatagcc ttattctcac cccataaacc ctttgctgta
    27361 tcttttccaa gattataaaa tcctttacag gcagaaatag caaaggcaag agcggtttca
    27421 gcgacacctt cgcctaaagc tattgcagaa cttgtcactc gaattttacg cttcaacaat
    27481 tcatcagata ttatcggttt aactgaacct gcagcatgaa ggacagcttt taaatcagga
    27541 cacacgtcaa gaatttcttt cgataaggaa ggtgtacccc atgaagttat aattatttca
    27601 gattctttta caaaatcaat aacataacct gaatctacaa aatcgtcttt atcatatata
    27661 aaaacgtcac cttgtttgcc aagtctgttt atatacttct cattaaacac tttttcctgc
    27721 attgctttat ttaccagtat tgtgatttta cccattttaa cctcttatca atagttgttt
    27781 ttactcatat aggctaatta caaatatatg aatatattag tctttataaa caataccttg
    27841 tgagtggcta taaaactttt caaattcata tccgtcaaat ttgtcattat cagtaagatt
    27901 atcaagatat actgccgttc cattcaattc atccctggat ttcttgccgc atagcattat
    27961 ttttgtacag ttagtaagag tatatatgtc tgaaaaaaca ttattgcgac cttccatata
    28021 atcgcaaaca gcttctagaa gggtacgata caaatcattg gtgtctatta caaaactgaa
    28081 agtacctttt gtcgtcataa cagtaatatg gaaaggctgc catttaccta tatgagaata
    28141 ataaacccct cttattccgt tttcatattc gatactgtac atttcacata tggcattatc
    28201 cccatctgtt ccttttccga tatattgaca acttattccc tttgctcctg caagtgctga
    28261 catagcttca actatatgca cagaataatt aaattcatca actccactcg ttccgaatat
    28321 actgattata tctccccgct cttcaacagg ttttgacaaa aattctttta tttcctttga
    28381 atatcttaat gaagaagaac ctaatatctt tgctccttct tcaaccagtt tctcaattct
    28441 ttcaatatcc ttcatgcttc caacaatcgg cttgtcaata aatacaggct ttcctttgct
    28501 tataaatggc atagcctgat caatatgttt ttcccagtta caggactgta taaaccctat
    28561 gtctaccttg tctgccattt cttcaattga acctgctctt gattttaagt tgaacctatt
    28621 tataaaccag ttaacctctt catctgaccg gaaaccatcg ttaaaaatat actcatacct
    28681 catattaata ttatgagatt caagattcga tgcaaatgct ttgggatgtg atacatcaat
    28741 attaactcca cctattttta tcataacaaa taccctttca ataactatca gtctattgaa
    28801 ttatataacc gaaacagaaa attttatata tgaaaatatt gtcttcttga tgataaataa
    28861 ttttatgaaa aacagttatg taaaattaac ttcctgtatt tatgttcttt ttgtcatata
    28921 tgaaaggatt atttatctag taaaataatg taaacacaaa cttctctaat ccctatagat
    28981 gatatatact acttatagcg aatagagata ttcaaaaaag attccacatt tatcatgcat
    29041 attttgacat ttgaacacaa ggaactgttt cctataacat taaaattagt gataaaacag
    29101 caaaatactt ttagaaggtg attagatgat atattacaca gcagatcagc atttcggaca
    29161 ttctaacatt atatatttat gtaacagacc ctttaaggac atatcaataa tggatgaatc
    29221 attaataatg aactggaata atactgtaac ctctgaggat atagtataca ttcttggaga
    29281 tttaattttc cgaagcgata agcatgctgc ttactatatt gaaagattaa acggcttaaa
    29341 acatttaatt ttaggtaacc atgaccataa atggattaaa aactgcaact tgagcaaata
    29401 tttcgcatct gtttctgatt atctgcaaat aaaggatggt gaaacccata ttgcactttg
    29461 tcactatcct atgctctcat ggagtggtgc agcacgaggt tctctacatg tccatgccca
    29521 tatacacaat ataagagaag gtcttgtttt tgaaacatta aggaaaatgg ataatgcttt
    29581 aaatgctgga gttgaggtaa acggatataa acctgtcaca ctgaaagagt taattctaaa
    29641 taatgctaaa tttaagatat cagattgaat aaaaatctac tcttttttct tttctttaat
    29701 aggaaacagt aaatccagtt gcatttcttc gtttttgaac tctgcatttt taagattatt
    29761 caaatagttc aattgttcct caaagcatct ttccatatcc atttcatatg gcgaacagcg
    29821 tattgaatgc cagtcatgtt catatttgtc attgttcata acccagtttc ttaataaaat
    29881 ccaatggtca aagtctccca tcttaatcat atgcgcagca taaagacctc catcaaatat
    29941 tcgcttaacc aaaggcttcg gtacaaccat atcttcaggg attgaaaccc acatctgata
    30001 tttatatgaa gattcaccta ctcctgtttt tcctgctgaa caatcaaatc caaagtgtcg
    30061 aatatcgggt ttaattttga gtaaattgtt ttccattaca aaacgattaa tcatatctgc
    30121 tgcttttcct tcacagtttt cacctgtcgc actatatgca gctactgtca ttggaggtag
    30181 atatataatc cttatgtcat taagcttgct tatcttctcg tttgcctgat ttagttcctc
    30241 catcgatttt tcctccttaa aatttatttt tgaaacagtt agggaattca caattcttaa
    30301 cagactatca ccattaagta accataattt atctgtatga acctgtagtc gctccaaaaa
    30361 ggcttgaatt acatttttta tcgtagacaa tgcggtaatt tcatcttcta tatcaataag
    30421 attttgttga aaaactttaa ttgttgcagc tgtatctttt ctttttaata tttcaacaat
    30481 ttgcttaagt gggattctga gtttacgtaa tataatgatt tgctgtaaat aaataacagt
    30541 attttcatca taaatacgat aagcataatc atcagttctt atagattgta ttaaccctat
    30601 ttgttcatag tatcgtaaag tccgtgttga gatattaaac atccttgata cttggcttat
    30661 tgtctttaat tccatgatct gtcctgcctt caattcaaag tatacctgtt gacgcgacgt
    30721 aaaagtcaag aggcagttat tatttttctt tattctttta ctttttacat aggttttaga
    30781 tttctgttaa gtctttcaat aattttcaaa aaatctcgtt cccgggcttc atctgtctta
    30841 aacgaaagaa atcttcttat gtaaatagtc ttaacagatt gcggcatatt attataattc
    30901 tcatatgcag gcgatattcc ttttagtttg tctgtaaaag tttttatctg ttcgtcagta
    30961 atcgactcac tcttaggaag gtcccaaata ccatttacct ttgctgtttc cactgctttt
    31021 tcgcctaatt ctgtcattaa cccttttttc tgtaggcttc ccacagttaa cttgttcttt
    31081 tcagaccagt tacttttagg acgtcgtttt gaaaaatatt taatatactt tgtattgtct
    31141 atgctcttca tctgtccgtc tatccatcca aaacataatg cttcctctaa agcatcattt
    31201 gcagataaag tgaccatctc ttttgttttg ctgaatacaa gaaacactcc atcacttttt
    31261 tctgcgtttt tgttaagcca tactctaaat tcatcacgtg atataaatag taattcagga
    31321 acatcttttc tcattacagc aactccttta cattaaaaat ctgatgttaa tgtatttaaa
    31381 atccttcttt ttctaatatt ttctttattc tatctgcagc gtctttcata aatacttcca
    31441 gctcttcaat tgtaaaatac cttattgggt ttttgtgcct acttactttt gtgtcaaaat
    31501 attttagtgt atgacttttc caagccgtta tttcgctttc ctctaataac ccttctacaa
    31561 aaaagctttc ggatttttta agcagactcc atatactttc acgccattca ctatttttat
    31621 acagttcaaa gtcaagctgg tcggtatcat tgtaatatgc tgattttaaa tcctgtactg
    31681 gattttgatc ctttatatat tcttgtttaa atttaatata tactgtttcc caccaaataa
    31741 tatcggttaa tatatgaatt ccataaccta ataaaaagtt ataattcttt ttatttctgt
    31801 tctgttttaa aaaattgatt acattatttt cccactcagg tccttctgca tgtaaatgag
    31861 taatcctttt atctttttta tcgatatttc cgcgtacatg aatagcatca ggtgatatac
    31921 agcctaaata gtattctgga caatgtaaca attcagaata atccttagca tatacccttg
    31981 ctgtaccaag atgcaccatt ggaagcggca ttcagatagt ctccttatat tcattaagta
    32041 aaattatata tctaataatc ccaaaaagcc aatattcctt caataacact aattaaatca
    32101 gcatttctta aaggtgcttc atttttgatt gataataact gtaagggatt aactaaatct
    32161 ctaaataagg ataactcata ccaattggaa ggaagctttt tatctgcata tgtattatat
    32221 attttctcaa ataaacgaat atcatcatta ctgaaaaatg tgcgatatct gaaaaactgt
    32281 ccaatatccg ctaaagtatg tccccacaaa gctgcttccc agtctatata gtaaatacta
    32341 ttcttttcat caacaagcat atttaatggt ctgaaatcac tatgtataaa tgatttatag
    32401 ctatctattt gcgctattaa ctcatattta tctgttacaa gcctatgtat tctcttacaa
    32461 agattcaaac ctaacctgtc tcttactgtc gggatatcta agaaaacctg ataccatatt
    32521 tcatagggcg gtatgtccaa ttctgctaac tttttggttt cttctattgg tgtactatgt
    32581 ataattgcag cagatttcgc tacttgttta agtagacttt tatcacatct tttgttatca
    32641 acaatatgct tctgcaaaga aacaccattt atatactgat atatatgata aatatattca
    32701 ccctttgttt tatagaaaag caactctgat acattaatcc ttccttttac tatttcaaat
    32761 gcagtaattt cgttattaaa actactttta tcagatattc tcagcagata tttatcttta
    32821 cttgtgttta caacatagtt acaattcctg catccgctct gaataggaga aaaatcaatg
    32881 attagaatat cattcttata ctcagaaaaa atatttccta tttcatcaac atctatatct
    32941 aaaatcggtt ttgaacgttc ccagtctaaa tcatgcattc tatatgctcc attgtttaag
    33001 tatatactat ttttaaattt attactttca tctgcagttt ttactttaaa tgtactaata
    33061 acaacttata ttaaaccctt caccttgcat acaaggtaca acagtatgtt ggtaaatcct
    33121 ctgtcttatt gtttcaaaca cagtataata catcattcct gggtttttaa cagaaaaaaa
    33181 catgtttttt aggttaatag ttaaaaataa atctgattca tcaaagatga aacaataaag
    33241 gattgaattt tatgtcgcaa atgttatata atacttgcga caggaggaat tgttttgggt
    33301 aatttatcat acaacaattt aatatataac cgaatattat ccttgccgcc tgaaagtgct
    33361 tttacagcaa cagattttgt tgatattgct gattacaaag cagtaagcaa atcacttgaa
    33421 cggcttgaag atgaaaagaa aatccgcaga gtgatccgtg gtgtttatga ctgtccaaaa
    33481 tacagcaaac ttcttggtga attcgaggct ccgtctcctc atcacatagc tatagctatt
    33541 gccagaaaca acaactggac cattgctcca agtggaaata ctgctcttaa ccaattaggg
    33601 ttatcgacgc aagttactgc atattggctg tacatcagtg atggtcccta taaaacatat
    33661 gatattaaca atgtaaaaat agagttcaag caccgcaata acaaagaaat atctggaatg
    33721 tcctataagg ccgcaatggt aattcaagca ataaaaactc tgaaacagca atatgtgagt
    33781 gaaggcatca taaaacattt gaaacagagc cttactgacg aagaaaaagc taaattactg
    33841 ggtgaagcaa aacaaactac gacatgggtt tattcaatta taaaaaaaat atgtaaaagt
    33901 ggggaataaa tatgtatgag gttgccagaa ccacgataaa tgaacggcgg gcattattca
    33961 gaaatacatc tcaaaaaatg aatgtccatg aagccataat agaaaaggat ttttgggtgt
    34021 gtcttactct tgattacctg tttcaaagat gtatttacaa gagccatctt gtttttaaag
    34081 gtgggacatg tttatcaaag gtctataatc tcattcaacg tttttcagag gatatagatt
    34141 tgattcttga ttggcgtgtt ctgggatatc gaatgactga accttgggat gtgcggagta
    34201 aaaccaaaca ggaacaattt aatagagagg caaatgaaag aactatatta ttcttacgtg
    34261 atactttgct tcctcaaata actgaggagc tatcagacat aattggtgag aaggctgaat
    34321 tttcaattaa taatgatgac cctcaaacaa taatcttcaa ttatcctcat atattcaacg
    34381 ctgaagctat cttgcaaggg ataaaacttg aaattggagc attggctgca tggaaccctg
    34441 taagcatgaa aacaattact ccatacgcag cagaaaagta tagcagagca tttaaaaaac
    34501 caaatacaga agttttgacc gttgaaccgg aacggacatt ttgggaaaaa ataactatct
    34561 tacaccatga agcaaaccgt ccggtgagct cattaatgcc acaaagatat tccaggcatt
    34621 attacgattt atactgtatt gccaaaacag aagttaaaga ccgggcaata agcaacattg
    34681 caatgttaac aaatgttgac gaattcaaaa tgaggtttta cccaagaagc tgggctagat
    34741 atcaagacgc aaagccgggt acattaaagc tcttgccacc tgctcataat ctaagactgt
    34801 tggaggagga ttacgaaaat atgggagaaa tgattttcgg accacgtccg ggattcaagg
    34861 aaatgatgga tgcaattacc aaacttgaaa atgaaattaa tgcatcatag aatgcatgga
    34921 aaatacttta aatgcaggtg tagaaataaa cgaatacaag ccaataaaat tagttgaatt
    34981 tatttcaaat aatataagtt atttgaatta aaagcatata ttaaagcttg gcatcatttt
    35041 gcataaatca gggcatatat cgaaaagtaa tattatttaa agaacataaa aaaccctaga
    35101 aactttgtgt tttcatgggt tttggcggag agagagagat tcgaactctc ggacggcttc
    35161 cactgtcaca cgatttccag tgcagtaaac caggaatttc ttcttatatt gtcacataac
    35221 tctttataac ttccctgctg aaaggcacat aaaaagctgg ttattttata ttttcttata
    35281 tctctttact tctttatata tattggtttt gtaaggttcg tcaccatttt gtcacccctg
    35341 gcgccatgaa aatttgaagg aggttttaca atgtataata taaaaacatc aactgatctg
    35401 agtcttctgc atgataaagg tattttactt tcattcgggg aaatatgctg gaataaaatc
    35461 aacctgatat ccggagcaat gactgcccca cttgttgaag caatatgggc ttttactgag
    35521 aatgacgctg aatctatgga aagaattttg gttaccttaa accatttata tcataatgat
    35581 cataaagttg aaatgatagc tgtattgcgt atcttatatg atgtttctgg gttgcagttt
    35641 ccggaagatg ttcaactgct agcagaacac cctaagacaa ggcaatactt tctgttctca
    35701 ttcttgctgg acatggatga ctgtatgcag gaatttatga ctgaagaatt aaaaggataa
    35761 aagcaaacga tatagagaaa tatgcctttt ctagcaaaca attgggttac atacaacaat
    35821 caaatacacc atacaagata tattattacc agaatataga attatatggt aatatgttgg
    35881 tgtgcttaaa gttatttcag gtacaccaat actaaaaata tgggtgagga gcgttactat
    35941 atggaattta atattctctc tgtagactca ttgatgttat caatagttgc aaattctttg
    36001 cataaacaca atattcatcg tggcagagac ttctttgaaa aatgtttaag tgaagaaaaa
    36061 agacgaagtg actgagcagt tactgaaatg gatatgagat gtatataaat atgatagaag
    36121 agttgatcat tggtttacag gagggtttat aatatgcatc aaataagcat attttcatgg
    36181 ttcggatttc ctattcctat ggaggaacga tttaaactga taaaggaagt agggtttgat
    36241 ggggtattac tatggtggag tgatgaatat gcagaagtag atggtgataa gttactgcac
    36301 ccggatttag caagacagaa aggcctattt attgaaaaca tacatactcc tattttccga
    36361 aataattatt tgtgggaaga cggtattgat ggagatgttt tagaaaagac tctattggaa
    36421 tgtattgaag attgcaaaag gtttgaaatt cctactgcag ttgtccatat tacacatggc
    36481 atcaatccgc acccttacaa ccaaattggt attagtcgaa tcaaacgtct tgttgaagtt
    36541 gcagaaagaa ataatatcaa tattgcactt gaaaatcttc gcagacctga gtaccttgat
    36601 tttatattta cacatataca ctcagacaga ctagggtttt gctatgattc tggtcatgag
    36661 aagtgctata caaaaggtac agacatgctt tccaaatatg gatcaagcct tatggcactt
    36721 cacctgcatg acaatgatgg aacagaagac cagcatctta ttccgggtga gggtttaatt
    36781 gattggggtg ttgtaaagaa aaagctttgt gaagtaaatt attcaggagc aattgctttg
    36841 gaaattacaa atgagttttc aaagaatatt gataatcaag atccatgggc gtttttgaaa
    36901 agagcctatg aagctgcaaa gagattgata ccatgacctg aatgctgttt tagtattcaa
    36961 gttttaggtt aagtactgta tctactgttt tttaataaag gattaatttt tgagcataag
    37021 ccttttggag ggatataaga tatggagaat tataatatag aattccgcct gtgagtacaa
    37081 ctattcgtca gtttatcagt caagctaaat aggagtgcag aatagaaaga gactgtaatg
    37141 tgcttatgct gaatgtgtaa ttggcaaata tgtaaaggat tataggccgt aaacaatata
    37201 aagtaaggat gagaagtaag gatgagtatt ataaaatcac atgataatac actctatgga
    37261 ggaaatggag aatttaatat tgaattaaag ccgctttgcg atgaacatct gccatattta
    37321 tataaatggt gctcagacct tgatgttctg tattggacag aaaccgatga agtaggtgaa
    37381 ccatatgcga aagaaacagt tcatgatatt tatggtggtg tttcgcaaaa tgccttatgt
    37441 tttctcattg aagtgaatgg cattgcaatc ggtgaatgct ggttgcagaa gatgaatatg
    37501 cccaatgtta ttgcgatgta ttctgaatat acagatgtcc gcaggattga tatggcaata
    37561 ggtgaaaaag attattggaa cagaggaata gggacacagc ttattcaaat gcttgttgac
    37621 tttgctttta acagtgagca ggttgaagtc ctacattgtt tttgtgaaga atataataag
    37681 cgcagtcaaa gagtgtggga gaaaaatgga tttatacttg aacataaaat aaagcatgcg
    37741 tacaaccaaa agtcgaatta tgaatatcat tatgtactca caaggcagca gtatatgcaa
    37801 aaccgtcatg taaaagcttg tgatgtataa ggttttatgc ttcctatact ggatatagta
    37861 acaatggcta ttaatgaaga attattaaat tggggggata aaaagagttt tccattgcca
    37921 aaagaattaa ttaagaaaga tctgaataca tcaattttat atatagatga aattcgtggt
    37981 ggaatttcaa aacaggtata taaaataaca acacagctca ataaatatat tctatacata
    38041 tagcttaacc cctatgagag taaaatcaca aaaaaacaga taaaaggaat ggaatatctg
    38101 tttccagatg ggtataagta tttcaaacat aatacaaaat taatgaacga tataaagatt
    38161 cgagtgccat aaactgtatt ttaacaaata ataaatttaa ctaaatttta ataatatctt
    38221 ctcttttggg gccattagat atccacttta caggaacccc tatagaatat tcaataaaac
    38281 ggacatagtt tcttgctgct tcgggtaaat tatgaaaatg ccttatgcca gatatatcgc
    38341 atttccagcc aggtaaaact ttatatacag gtctggaaca gctaagaatg cttgtgaccg
    38401 gaaattcttc aataatagtt ccatcaattt catatcctgt acatacagaa atttcatcaa
    38461 gatatcccaa aacatccagc aatgtcaatg ctgcttcggt agcaccttga accatacaac
    38521 catatcgtgt tgccactgca tcaaaccacc ccatcctccg aggacgacct gtggttgcac
    38581 catattcacc ggaatcgcct cctttttcac gtagtactga agctgcatca ccgtggattt
    38641 cggttacaaa tggtcctgct cctacacaac ttgaatatgc ttttattact gctgttattt
    38701 tattaatttt atagggtggt atacctgccc caacagcacc aaacccagca agaggcgatg
    38761 atgatgtagt atatgggtat ataccaagat ctggatcttt taatgcgcct aattgtcctt
    38821 caagtagaat gtttttaccc tgattaataa ctttattaag caaaatggtc gtgtcacata
    38881 tcatatgttt gattttgtct ccttgagata ataattcttc aaataccata tccgaattca
    38941 aaatctcagc gttatataaa tgtttgagta ttacattctt tactgcacaa atattgaaaa
    39001 ctttttcttt tagtgtttca ggactatata gatcacatac ctgaattcca attttactgt
    39061 atttatctga gtaaaaggga gcaattcccg actttgttga tccgaagctc ttgctgccaa
    39121 gcctttcttc ttcaagcttg tcaaaagtaa tatgccatgg tgttataact tgagcacgtt
    39181 cggaaatata caactttgga tccggaacat ttttatcctt tagattcttc aattcatcca
    39241 taagaacact aatgttcaag gctactccag gaccaaggat attggtaatg tgtttatgga
    39301 aaacgcctga tggaagtata tgaagcgcaa atttaccata ctcatttatt atagtatgac
    39361 ctgcattgtt accaccttga aaccttacta cataatctga ttcaactgca agcatatcgg
    39421 tcatttttcc ttttccttcg tcaccccagt tagcacctac tatagccctt accatatgat
    39481 ttcctccttg taaatatagt tagtatttgt ataaacctat gaggcatgaa cacaactgca
    39541 ttatatattc aaagctatta taagtaaaat taatattaat taactgatgc ataatcaata
    39601 attatgtcta tcagatatct ttattgcttt ttctggtaat gcattaaata aggaacttat
    39661 agatatattg ttatgaattc gaagaatgac ttctgaaaaa agtggtacag cagatactac
    39721 ttttatttta ctagataaat taatgcttgg gttgtatatg gaatcagtag taataaacat
    39781 gcttatttcg ctacggtcaa gctgttgtat gcttttttcg ttactaagaa tatgtgaagt
    39841 gcatgaaatt attttctgag ccccaagttt ttttaactta tatgacaagt caatgagagt
    39901 gcctccgctg ataatgaaat catctacaat aactatattt tttcctttaa catcacctat
    39961 aacatccaga atatctgcct tttcattatg tccaactctt gccttgtcag cgataacagt
    40021 agatacaccc aaatatttag agtattcccg ggcttgtttt gcatacccag catcaggaga
    40081 tacaatcaca taattctcta aattcagcag ctttatttgt tcacaaaata gaagcattgc
    40141 ttgcaaatga tctactggtt ttttaaagaa accttgtatc tgagggctgt gtagatccat
    40201 ggtaataatc ctatctgccc ctgcaagttc tatacattca gcacataccc ttgctcttat
    40261 tgaaaccctc ggttcgtcct tcttgtctcc ttttgcatag ctgaagtatg ggatgattgc
    40321 tgttactgat tttgcacttg ctcttttgaa tgcatccatc cagaataata tttcaacaaa
    40381 ttcatcattt ggtctaagtc ctatggactg aacaaggtat acatccttgc cgcgaactgg
    40441 ttcattgatt cttacatagg tattgccttc agaaaatgtt attacgtcag aatcaccctt
    40501 tataatgtta aggtaattac atattttgtt tgtaaaatct accgaagaac ttccgcagaa
    40561 gattctcata tcatatttca tcttgttccc ctccaagtat ctatttattt acatcaatat
    40621 gataactcaa agaggatacc taaatataat tgtttatttt tatatatatt ataaccttta
    40681 cttatcaagc tttactattt atcaactctg taaatctgac agcagctgca gataggggag
    40741 tatttttaag ctttattata cctatctgtc tgggagggat cttttctatc agatcaagct
    40801 caaaaatact tccattttcc aggtcatttt ctgcgaaatt ttttactaca caggatatac
    40861 ctaaacccct gcgtgcaaac tgtacaagaa ggtcactagt tgcaagctct atttcaggga
    40921 taatagttat cccatatgaa taaagaaaat gatctatata ccttctggtg cttgtgttct
    40981 tttcgagcat aattacaggg taatttaata attccttcag tgaaatatta tttgaagcta
    41041 aatgtccgaa tttataattt gcaataaaac agtcctgaat ctccatagct tcaataacat
    41101 ctgttgcact gtcatcaaaa agcggtaagc tgactattcc aaaatctatt aatccgtttt
    41161 taagacaagc tacagtttca ggtgagggac catttgtaac ttttatcctt acttttggat
    41221 aatgtttatg aaattcttca agatgaggca gaagaaagta cttgagtgtc atgtcgcttg
    41281 cacctattcg aatttctcct gcatcaagat taatggcttc tgaaaacttg ctttctgcca
    41341 agagaatcat attataaccc tgctctatgt atttatacag aacctcccct tcaggagtaa
    41401 gtgttattcc cttgggtgtt cttatgaata actgacctcc aatctttgtt tccagctgtc
    41461 gtatggactg actcacagca ggttgagaaa tataaagctc ttgtgaggcc tttgtaacac
    41521 ttcctgccct aacaacataa tagaacacct tatacaattc cagattaaca tccatataac
    41581 tactccttat attaacaatg tctaatataa ttaataatga tgtattataa ttatactgta
    41641 acatacagca tacttccata gatttattta ttttgttttt ttgtattgat tctagtactt
    41701 atataaaata gtataattat tgaaaaacca atatatgatt tgttggcaaa gtattagcaa
    41761 attcaccata tacatacaaa aagacttcat gttaaagcat tgaagtcctt tagtctggcg
    41821 gagagacagg gattcgaacc ctggttacta ttcctagtaa acacgatttc cagtcgtgcg
    41881 ccttagacca gctcagccat ctctccaaac gcaaatcata ttttatacac cagcatatga
    41941 tatgtcaatg aaatattacg agtaatagta atccgttagc ttttgggaac acttttctag
    42001 tagctctttg tcatatttat ttagatgagt aagaactgtg tacttggttg tgctcatgat
    42061 gtggccatat attatactgt ctttaaatat atcttcagga atgcctaatg ctttagggtc
    42121 tgtttcgcag cccgcattct ttaatatttc ttctatcttt tcagtttcta tatccatgac
    42181 ttttattgga agcttatctt ttacaatatc ataaagtctg cagataacgc ataatgctac
    42241 gccgacagaa tttccatgta aagggtgttc ccatcctctt tttatgaatt caacatccca
    42301 gtaatgcaca agatgatgct ccgctcctga accaggtctt gtattagtat acatacccat
    42361 ggctattcct gaaagtataa gcgcttccat tatattttcc actgcttttt tatctctttg
    42421 ttttaaaaga gcagcactgt ctgaacattt gtcagcagct ttccatataa gcccggctat
    42481 ttcgtcgcag taatgctctt tattaagatc ccttgaaaat ctccactccc ttaccgcatt
    42541 gtactttgac aatacatcac caaatcctgc tgtaagcatg gtatatggtg cattttttaa
    42601 tatatttatg tctgcaaaaa ttcctatagg gacctgcccg ggacagcttt ccttaagatt
    42661 atttataatt agtgctgatg taattgaagc atatccgtcc atagatggag cagttcctat
    42721 aactacatac ttttttccta aatgataaga gatatttctt actatatcat taacaactcc
    42781 gcttcctact gctacaaaga agtcaatttc taacggcatc cttattaaag tttccccaat
    42841 tgtactttca ttaggtacaa cagcaacatc atttttctga ggaataatat agcttagggt
    42901 gtctttgttc ttttctttta gtttttcaaa cacccttcct ccggctattt tgaatgtaat
    42961 ctcatcagat attaccatta tccttccttt tgctttttca agaatgaatt tgatagcttc
    43021 ttctattccg ttttctgaga tctctattct ttccattcca gggtcatgta caagcccgca
    43081 ttcacatgga tatgtgtttt taattaattc cttaatttcc aaatctttaa attgcatact
    43141 cacctctagt ttatcaattt caataattca tatagtccga atcccataaa gttaccgata
    43201 gcataaccta aaagtccagt cgctatacct ataagtatta caccttcgtt cttaatcgcc
    43261 ttagcagtcg gtattataaa tgccggtcca aatattcctg caatagaagt aatgataatg
    43321 gtatcaacat caatcttaaa caacaaggat ataatgaaat gtaaaagcat tgttgcaaaa
    43381 agaacaaatg ctaccattcc cagcacctga acagattggc cgacaataag ttttatatct
    43441 gctaatgaac ctacagctat tgaaaataca tatatgaaat actgtcctaa tggatacgat
    43501 atctgatttt gccttatctt tttaacaaat gataatgtaa cagcaataac tgatacagat
    43561 accattatta cagcaacatt agtcgcttgt tttgtaagta aaaaagatat tcctatactg
    43621 gctccaagag ctcctattcc gataagaagt gcaactacac cctgctttat tttttcttta
    43681 gtcgaaagtg atgttttact tataatgctt tccttttcat aattagtttt ttttgctgct
    43741 actgatacct gcttagttaa tttggttaat atcggtttag ccagagtaca tataaacaag
    43801 aagtacagtc ctcccattac ctggtctgct aagtttgcaa caatgattgt gttcttgtcg
    43861 gtgttcaatc cgtatcctat tgcaaaaaga ttaggagttc ctcccgtata gcaccccaca
    43921 agcattgatg caattttagt gttttcatat acatttggaa atacaacaac acttaatgct
    43981 gctattgaaa gaatagatat acataatatc aaaaaagata aacctgtttt ttttacattc
    44041 ttttttatag tcaaaaaatc agaatttaac aaaattaatg taatagctat cggaatcaga
    44101 acagaagaaa ctgtatctga cagagaggca tcaagaggaa atccgctatt tgcccagata
    44161 atacctactg cataacaaaa cagtatcggt ccgattgttt taataaattt aactttttcc
    44221 tgcagattaa taagaagata tggaattccg aaaaaaatta taagctgaat tacagtcaat
    44281 accataataa atccccctct tactgattaa tatatattat atatttattt ttgtccattt
    44341 tcccattttg aatcttattc gccctgccat tcctcttact aattgatcag aaaacaagcc
    44401 taaccatgct cctattaatc ctaaaccaag cggatatgtg agaagatatg aaattccggg
    44461 tcgtattact gcaacagaaa ttaatgccat tgttgcaaca tatcttacat cacccgcccc
    44521 tcttaagcaa ccagaataaa caatctgaga atttataaag aaagtaactg cagccattat
    44581 aattagtatc ttgctaccgt tagctataat acttggatca tctgtaaata atcccataag
    44641 catctttcct ccaaagataa acaggcttga aagcgctatt gatattataa aagccatcct
    44701 ttgtcctatt tttacataca aaacagacag atcactcctc ttttctccca tagtctgtcc
    44761 aactaaagct gttgtgccga tacctaaacc gctacctata tttgtagaca aattcaatat
    44821 atttatacat acaagatgag ttccgtaatc aacagttcct aattcagcaa taatcttatt
    44881 atacataaag aatcctactc ttaagcatag ttcttctatt gctgcattac cagaaatttt
    44941 aataatggat ttaactaatt tcaaatcgaa gtttttaaga tttttaaaat tgaaaaagat
    45001 aaaagtagat tttctcaatg ataaacatgc cattacaaat gctgttagta ttcccaaaaa
    45061 tgttgctatc gctgctcctt taacacccaa agcaggaaat cctaatcttc ctttaataag
    45121 gagaaaattt aaaaccacat tgacaatatt tgcagctata tttgttttca tggctacttt
    45181 tgtattacca gcacctcttt gagctgcgtt aattgtcatt gtcagagcca ttaatacagt
    45241 tcctgcagtt acaataatat aatatatgac tgcatcatca ataatatcat caccagcacc
    45301 tgcaaatttt aaaaggggtc ttgctgttgc aattccaaca accgtcataa ttattgcaag
    45361 tataaggcaa attaaaagag actgcatcaa aatctgattg gctttttttg catcattttc
    45421 gcctcttctt ctggcgataa gagccgtaac tcctgtatta agagccatga agagcattaa
    45481 tataatcatt ctaggctgag tagtgattcc aaccgacgaa acagcagata tgccaatagc
    45541 agataccatt atagtgtcta caaaacttgt caatcctccc aaaagagctt ctacagtgga
    45601 aggaaatgcc attctgaaca tccttccgta agcctctttc gcagtgggta cttttcctaa
    45661 taatctttgc tttcttagca aataccgtgg ctggtaaagc tcctttatca tctttaacat
    45721 tctaacatat atttctcttt ttgttatcgg tataattaaa tatataagga agtgatataa
    45781 tttagtaaaa ctacgagaaa ggctgttttt atgaaaatac aaaaagtttt tatggataat
    45841 atgcaatctt acactgattg ttttaaaaaa gtctttgaaa ttcaaggaag atatgtaaat
    45901 gatcactgtt tcataataca caataatgaa atacatcttt tctatataga tggagaaact
    45961 ggaaaaggct gttatgatat tggaaatgaa atcattatcg gtcatgcagt aagtaaagat
    46021 ttaatatcat gggaaaaaaa acagcctgca cttgtttacg atcaatcatt gtcttttgag
    46081 gaacgaggaa ttttcgctcc ttatgtatat aaaaaagaaa gcagattcta tatgttctat
    46141 tcctcacata acataaaaaa agcacagttt atttgccttg ctttctctga caatcttaac
    46201 acctggcaga gatatgaacg gaatccttta tttaaaccgt cttctgattg gatcttttgg
    46261 gatgattctg taccttgctc ctgtagagat cctcatattt tctatgatga agataaaaaa
    46321 gagtatattt tatactgggt tgcagacata aaagaaaata ataagctttc atgcatagca
    46381 tcgtccatct caaataatct tttttcttgg caggaaaccg ggcctgtgtt gattagagag
    46441 cattcttttt atgaaagcat tacctgtaaa actgagtcac cctgtttaat taaaaaagat
    46501 aatctttatt acctatttta cagacatgga aatggaacaa agtactgcat atcggattct
    46561 tacacaaatt ttactcacag tgattcatat tacttaggtc cttctcatgc aagcgaaata
    46621 tttatgttca aaaatcaatg gtatatatcc agctgttcaa gaaaaattgc tgatctcgaa
    46681 cacaagactg accgtagcaa agggcttttt ttagcccgtt tatcatggaa tggtttacac
    46741 ccggaaatat actcattatg aatattatgt ttagcaagtt tagaaaaaac agaaacacat
    46801 gattttcagt ttaaaacact atagtacata gttgagctaa taattaattt attttaagtt
    46861 atcgtcaatt atttttctta tatcaagttt cttgttgttt cttccgcatt cataaaagtc
    46921 ttttttataa ataattgatg cccagcttat agctgaatcc attatttttg tctttacacc
    46981 catttctttt gctaaacagc ttattggaac acatcctgtt tgaacatctt catgaatata
    47041 ccttgtatca atatcaaaag gagctaaaac gcttgaatat gcatgagtat tctgtataca
    47101 ttcatataga ttattaccgg tagacccata tctttcttta agccattcac acacagacaa
    47161 taattcaatt cctgcttttc tcgcagtctc cagtctttct ttatctaatt cttccaatat
    47221 gtttgcaact agtggagaaa ttccatcaat atagtatctg aacacttcct ttgcttcaat
    47281 tcgtgtaata ttcattaagg ttggcaaagg atggaatatc actccaatat tggataatcc
    47341 tgtatggagt acacttggtg cagattccat gtcatgaaag atttcgttca ttatgtcagt
    47401 aatttctttt gtataagcct ttttatgacc tgcaatttta acttttttct tgatttcata
    47461 tatatgtgga ttccctggtt tatcacaacg gcaggtaaac actaaagtat ctgcttcacc
    47521 tagtaaaata tccgcattac agccattttg ttctaaaacc tgattaaatt cgtatgttcc
    47581 aaacgttctg cctggattca aaactacaac ttgccctgtt tttagataca aagccatttc
    47641 ttttgctaca gcaaaatgat attgtgcagg tgtagtcaca aggataagat atgcatcttt
    47701 gattacttct tccatatcac aactaattaa tcctatgctt gctacatcat tgataatgcc
    47761 tttaatgtga aacaggttat ttgcaaacat atcaacacgc tctttctctc tagcatacaa
    47821 agagacataa taccccttta gagcaataaa tgcggctatt gcttgtccgc cattgcctgt
    47881 acttattact gctatttttt tgttcaatca aatttccttt cctgtaaggc gaaatagcct
    47941 ttgtttctca ttaagaaaac atctttcttt tggatcatat gccagacatt ttccttcata
    48001 aactcttgta ttgagcgttc cggtatattt atcatttttt aatatatgtg gagcatctaa
    48061 tattcctttt tttatgcaat ctgttaaaac cgctgcatct gctaatggat cttcactttt
    48121 atcttcatat agtttttcaa taaattttat taaatattca gcttctatga ttagctctct
    48181 ttttcttgac tgaactttca aatcctgttt tgcatcagca tttccatata atacagacct
    48241 tacaactcct cttactatct tacaactttc aattacaaca tcagctgaag ctgcttttaa
    48301 accctcactg tatccaacaa catgtattat atgtggttct attgccatcg agagttgcgt
    48361 tgatgcagcc aattgccctt ttgcaatatc aatatctgaa cttaaaaatg gcaatccggc
    48421 tcttacttgc cgataaactc tgaattcgtt tgttttaagg gattctgtca tttccacttg
    48481 cgctagtact ttagccaagt ccatggaaaa tgttaatgaa ttaggaatat taaacatata
    48541 ctgtgatatg tagtccttaa cactgttttt ctttgcatta taggcgctta tgtatgacat
    48601 agccacagac acaacatcat gtgcatccct taatccccaa tggtgaggct cattaagctc
    48661 aactggaatt ccccgttccc catgccatct cataagtctt tgagcgtcat ccatagaaac
    48721 atgaagtttt cttgttccac gtccatcaat ttcattatac caacatagag gtactgcaca
    48781 ccaggcattg gatattgact gatgaagcgt atccgcgaaa tttattacat catctgttcc
    48841 actataacat ctcattaatg gaaaattacc aaacctagaa gcttttttta aatcttcaaa
    48901 ctgttctttt gttcttacag gcacaccccc tgcaccatcc atatccttgt tcctcatctc
    48961 cggtctgaaa aagtactgct gtgtgttttg atctattcct agcgatatta tgtcaagcac
    49021 ttttgctttt gctattgttt ctattccttt ctttgtttct tcatatgaag gaagaccgaa
    49081 atggtgtcta agaagaggat atggcttttt ctgctctatt cttgacacaa tatcctgcgc
    49141 ataatgctct atgtttgtat cttttgaata tatacctgtt cttaagaaac ttatacaatc
    49201 atcaatatct tctgtcccat caaaaacaac atcaaaaaat cctgattttt ctgctatttc
    49261 ggctacaggt ggagtaccac cgaaaaccca taatggtttc ttttcaagtt ttttgttcaa
    49321 atttatgatt tcaggcaata atttttcaaa attctctgct gtaagcctat acccgactgc
    49381 tacatatctt ggtgagttga gctttattgc ttcaaacagt acatcagttc taacagctgg
    49441 tcctagaaaa accgtatcat atccttcgtc ttgtgcaagg cttaaaaagt gtattactcc
    49501 tgctacatgc acacaatttc caacagatgc tcctattatt tttccttttt tcatattaaa
    49561 tacctttatt tatttatcaa tttctgttga ataatcgaaa gcatcaagaa caaatactgt
    49621 tgcaccacca acaacatatt ctacaggatc catatttata tattcaccat gtgaacctat
    49681 tgaatatgga ggaataggat ctaaaaccct tctcttttta caattctctt taataattct
    49741 tagagctcca tcaacacgat tttcttccac accaagcatc aaagttgtat tacgtgcttt
    49801 taaaaaacct cctgtagaag caagctttgt tgctttatac tttttttcaa ttaattttga
    49861 tagtaaattt tttacatccg cttcctgtat aactgctagt atcagtttca tatcaattac
    49921 cccctccttg caaaacagat gttttgccat ttatgtaatc agctgctttt tgcactgctt
    49981 tttgagaacc gactattaca actgtatcat tttcatatat ttctgccttt ggtcctggag
    50041 aaataatcaa attttttcct cttcttatag caacaattgt tgcatctgta ttttgccaaa
    50101 attttatgtc ttcaatgttc tttccgacaa tacttgaatc tagtgatacc tttatctcgt
    50161 aatacgggat atttggatta ctatatgata ttgtactttg caatttaatt atttttgcta
    50221 aaagatcatg catttccctg tttatttcat catttttttc aattaaatcc ttcaattctg
    50281 actgcatatc catttgatcc ttcatctcat caaaacttga taaatatctt ttagcattat
    50341 cttttgaaag tatattaact ccgctgtttt tcttaacata aacaactttc atatccacaa
    50401 gtagtgctaa tgctcttctt attgtttcag gagatacctt atattctgat gataataatg
    50461 atcttcctga tatcatctgg ttttccgtcc attcattgtc agttattctt ttagctatat
    50521 ctcttgctat catttgatac tgaggattga ttcgttgatt cggcataata gtcacctttt
    50581 aatatttttg ggtacagata tattttacac tgacatatta taaatgtaaa tagtatgtcg
    50641 gtaaagtttt tctacttatc cacagatttt ctgtggataa ctcgttttct gtggataact
    50701 ctattttttt tataaatttt ctttaaaatt attgaaattt ccccttgtac tgtcttaaaa
    50761 tctttgatat attatattcc cttgttgata acattacttt tatccacagg tcacaaaaga
    50821 taaatctgct tagggtggta taatataaat agcagtttta ttttttttat ttattagggg
    50881 aaactaacat ttatgactgt acaagagatt tggaatgatg cattagtcaa tatcgaaagc
    50941 gttatgggcg aactcaactt taaaacttgg attcttccat tagaaccata ctctatagat
    51001 ggagacaatt ttgttttagt aacacaatct gatttttcaa gaaattatct tacaaactat
    51061 aaacttcttt taaaaaatgc aatatctgat gctgcagcaa aagatttgag tccgcttttt
    51121 aaactgaaaa atgaaataag agagtacgaa gaaaaaggta gaacttttat gccgaagaat
    51181 aatacatata tggattctca gcttcttgat aaatacgttt tctcaaattt cattactgga
    51241 tcctctaatc agttcgcacg tgcagctgct gaagctgttg tagataatcc aggaactaca
    51301 tacaatcctt tatttttcta cggagaaagc ggtattggaa aaacacactt aatgcatgcg
    51361 attggtaatg caattgcaga agaatttcct gataagaaaa ttttatatgt tcaaagtgag
    51421 actttcacaa atgagatgat ttcagctata aaaacagcaa aaagcaagga atttcgtgaa
    51481 aaatacagaa atttagacgt tttacttata gatgatattc aattcataac aggtaaagag
    51541 ggaacacagg aagagttttt ctatacattt gaaaaactat attcagctaa taaacagatt
    51601 gtaattacat ctgatgtccc tccggaaaaa atgagccgtc ttgaagaaag attgaaaaca
    51661 agatttcaac agggtcttat ggctgatatg caaactcccg attatgaaac gagagttgct
    51721 atactagaac agaactcttt aaagtcagaa tataatattt ctaaagatgt tattgaattt
    51781 attgctaaaa acatttcatc aaatgtcaga aatcttcaag gagctctgaa taagactgtt
    51841 gctcatgcaa gagtttttgg agatgatatt acaattgatt tagcaaagaa agctcttaag
    51901 gatatgattg atgaaaataa cgtaaatgac ataactattc ccagtattat tgatactgtt
    51961 gcaagatact ataatataac aacaaaggat ataatatctc caacaagaaa aagtaatatt
    52021 gcttttccaa gacagatagc tatgtacata tgtagaatta aaacgtctta ttccttgcct
    52081 gttataggta aatattttgc tgacagagat catactacag ttttatatgc cgttgacaag
    52141 gttgcagaaa aaattaaaac cgataaagaa gttgaaaata acgtaaatga gcttcttgat
    52201 aagctaagtt attaacagct tcctgtggaa atgttaataa ctttctgtgg aatattgtat
    52261 aataacagag taattaaagt tttcaatatt tatacacaaa agttatcaac agcaaaaaag
    52321 ccgttaaata aggcagatta tgagatataa acaatgtaca caggccctac tactactgct
    52381 gttagataag aagataagaa agaagaggtt taaaagatga atttcacatg tacaagaaaa
    52441 aaaattcaag aagctgttaa cagtgtttca aaggcaattt caaataatcc tataatgcct
    52501 attcttggta atatttttgt aaaggctaaa gatagtcaga taatacttag agggtatgat
    52561 cttgaaattg gtattgaaca catagaaaat gcacaggtag aagaggaagg agaagtactt
    52621 gttaattcaa gacttttttc tgatattgta agaaatatag gagaagaaac agttaatata
    52681 tatacaaaag acagtaatct ggtaataata tcaggtaatg cagtatttaa tataagatgg
    52741 atagaatctt cttcatttcc gaaactacca gatgtagaaa aagaaaataa agtaacaata
    52801 aaagcagaat atcttaaaaa tatgataaga aacacagttt tctctgttag tacaagcgag
    52861 caacgtatga tattgacagg agctttattt gaaagtgatg ataattattt tcaaatggta
    52921 acattagata gttttagaat gagtataaga aaagaatata ttcttggaaa tgaagaaaaa
    52981 ttcaagtttg ttattcctgg aaaaacatta aatgaattat taaaactcat aactaatgat
    53041 gatgaagaaa taaaagtatt ttttgaaaca acacaggttc tttttgaatt tggaaactgt
    53101 aaatttatat caaaactatt acaaggtgaa ttttttaatt ataaagcaat aattccaaaa
    53161 gattttgtaa ctaaaataga aattgatgtt aatgaattca gagaaataat tgaaagaaac
    53221 atgcttgtat cagatgatgg aagaagagct ccattagtat tagatataaa agataatatt
    53281 ttaacagcta atactcaagg tgaatctgga aatgtaaccg aaactcaaat tatcaaaaaa
    53341 gatggaaaag atatgagaat aggatttaat gctaagtatc ttttagatgc aattaaatcg
    53401 ctagaagagg agaaggtaag aatttctttt atgggagaaa caaatgcatg tgttatatcc
    53461 ccattaaata cagatgaatt cctatatctg atacttccag taagaatagc ctcataaggt
    53521 tgaataatgt tcattaataa tattactctg aaagatttca gaaattacga atatcaggaa
    53581 atatttccta aaaaaggcat aaatattatt tatggaagaa atgctcaagg taaaacaaat
    53641 atccttgagt ctattttttt atgttctgca gcaaggtctc atagaacgaa taaggacact
    53701 gatttaatta gaaatgatca gaaagggtat tatgtgcaaa ttgaaggaaa aagagatgaa
    53761 cagccttttt ctataagcat aaattgtgat aaagtttcat caaaaacaaa taaaagagtt
    53821 ataaaagtta atgatataag cttaacaaga acaggacagc ttatgggagt tcttaattgc
    53881 gtaatgtttt caccagaaga tttaacagta ataaaagaag gacctagcga aagaagaagg
    53941 tttcttgata ttttaattag tcagattaat ccttcatatt tttatgctct gcaagagtat
    54001 tttaaaataa ttaaacaaaa aaatgcatta ctaaagaaca tatcagaaaa tgggagaaac
    54061 aaagagttgc ttgatgtcta taatataaag caagcaaaaa caggatacaa aattatcaaa
    54121 gagcgaaaaa tatttattga acaaattaat aagaagtcta aaattaacca ttcagatata
    54181 acaaaaggat cagaagaact taatataagt tattcttgtg atttatctga tttttcagac
    54241 ggtgaagatg aattttatag aatgcttaca aaaagtgctg ataaggaaat taagatacaa
    54301 aactccatat tagggccaca cagagatgat attgatatta ttcttgataa tatgagcata
    54361 aaagaatatg gttcacaagg acagcagaga actgccatat tatcccttaa gttttcagaa
    54421 atgcaaataa taaaggaaaa aactggaaat aatccagtat tattacttga tgatgcctta
    54481 agtgaattag atttattaag gaaaagaaca ttactagata agattaatac aaatcagact
    54541 tttataactt gtactgaaaa aagaaagtat atgaccttag atagtaatat aagttttttt
    54601 agtgtaacaa agggaacagt taaaagcata tagaaaaaaa tacttaaaaa tgatataata
    54661 caaagaagta ttacgagagg attttatgtt tttacacata ggtgatgatt tagttttacg
    54721 tgaacaggat attatcggca tttttgattt tgaaacgtcg acattatcta aacacacgaa
    54781 aaaatattta caagataaag aaaaaaagca cagtacatta gctgtaagcg aacagatacc
    54841 taaggcattt gttgtagtgc aaaaaaaaga acaaaaagaa ccaaaagtgt attttacatt
    54901 agtttcatca ataacactaa aaaaaagagc agagaataat agattaatgg agtaatatat
    54961 ggaaaacaat aaaagacaag agaatattaa ggcctgtaac tataatgaag cacagataca
    55021 gatacttgaa ggattagaag cagtaaggaa aagacctggt atgtatattg gtactacatc
    55081 agtaaaaggt cttcatcatt tagtatatga aattgtagcg aatagcgtgg atgaagcact
    55141 tgccggaaga tgtgacaaga tagagatatt tattgacaaa gacaacatta ttacagttgt
    55201 agacaacgga agcggtattc cagttggaat tcatccaaaa atgggaatac caacagttga
    55261 agttgtacat actgttttac atgcaggagg taaatttgat tctaaagcat acaatatttc
    55321 tggtggatta cacggtgtag gagcttcagt cgtaaacgct ttgagcgaat ttcttgaagt
    55381 tgaagtaaag agagatggaa aaatatacag acagagatac gaaagaggaa aaactgtaac
    55441 acctcttgaa gtaataggta aaacagatga aaccggtaca aaaactgttt tcaaagcaga
    55501 taatcagata tttgatacag ttgagtttga atatgatgcc ttattgtcta gatatagaga
    55561 aatggcattc cttaataaag gagttatgat tatattaaca gacagaagag gagacgaaga
    55621 agtatcaaac gaattgtatt acgaaggagg aattgtatct tttgtcaggt atttagacgg
    55681 taataaagca agaatacaca aaaataccat ttatattgaa gaaacgaaag aagactgtat
    55741 agttgaaatt gctatgcaat ataatgaaac atatagtgaa actctgtatt catacgcaaa
    55801 taatattgcc acaatagaag gcggaatgca tgtaacagga tttcgatcag ctttaacaaa
    55861 ggtaataaat gattatgcaa gaaaaatagg ggcactgaaa gagtcaaata aaaatcttac
    55921 tggagatgat gtaagagaag gtcttactgt tgtaatttct gtgaaacttc atgatcctca
    55981 gtttgaagga cagacaaaat ccaaattagg aaatagtatt ataaggacaa taactgaaca
    56041 gacagtaaat gaagatttag ctgcatattt tgaagaaaat ccacaagaag gaaaagcaat
    56101 agtggacaaa tgtctattag cttcaagagc aagagaagct gcaagaggag caagagatct
    56161 tgcaagaagg aaaactgctc tggaaggaaa tgctcttcct ggtaaattag ctgattgttc
    56221 tgaaactgac ccagtaaaat gtgaaatatt cattgttgaa ggagatagtg ctggtggtag
    56281 tgcaaaacag ggaagagaaa gaaaatatca ggcgatactt ccattatggg gaaaaatgct
    56341 taatgttgaa aaagccagaa tagataaaat ctataataat gacaaattac atccactaat
    56401 tactgcttta ggtacaggaa taggtgaaga ttttaatatt gagaagctta gatatcataa
    56461 agtaattata atggcagatg ctgatgtaga cggagcgcat attggtatgc ttcttattac
    56521 tttcatatac agatatatga gagaattaat agaaaagggt tttttatacc ttgctcaacc
    56581 gccactatat aaaattacta agggaaaaga atttgtatat gcatttaatg atgcagaaat
    56641 ggaaagaata tttagagata aaggatggga tagaaaagag tgttctcttc aaagatataa
    56701 aggtcttgga gaaatgagtt ctactcaatt atgggatact actatgaatc ctgaaacaag
    56761 gacaattgtt caattgaaaa tggataatcc atttttagca gatattgctg tgtcagacct
    56821 tatgggagat gatgtacaac ctagaaaaga gttcatcgaa agacacgcta aagatgtaca
    56881 attaaataga ttagatatat aatgtttcac gtgaaacatt aaatgaggta aaaatgggca
    56941 aagtaatggc ggttgccaat caaaaaggcg gagttggcaa aactactaca tcgattaact
    57001 taagtgcaag tcttgcatat atgggaaaaa agacattact tatcgataat gatccacaag
    57061 gtaatgcaac aagtggactc ggtattgaca agtggactgt tgaaatgtct gtttatgatg
    57121 caataattaa tgatattgat attaaagaaa caattttacc aactatgatg gataatcttt
    57181 atatatgtcc atcaaatata cagttagctg gagcagaaat cgaactggta agccagatga
    57241 ctcgtgaaaa tagattaaag aatgcaatag ctacaataaa agaacaatat gattatataa
    57301 tcattgactg tccgccttct ttaggacttt taactttaaa ttctcttaca gcagcagata
    57361 caatattagt accaatccaa tgtgagtatt atgctcttga aggattaaag caattaatga
    57421 aaacagtaag tttagtacag aagcatctta atccttcatt aaaagttgaa ggagtagtaa
    57481 tgactatgta tgattcaagg acaaatttat cttttcaagt tgttgaaagt gtaagagaac
    57541 atttttctga cagagtatac agaacaataa taccaagaaa cgtaagactt agtgaggctc
    57601 caagtcatgg tgttcctatt attctttatg ataaagattc aaagggagct ttaagttatc
    57661 ttgacttagc tgaagaagta attcaatgcg gaggaagaaa tggctaaata cggtttgggt
    57721 aaaggattag gagcattatt agacataagc gatgataata ataagaatca gcttattgaa
    57781 ttagatattg ataaaataca aagagatcct aaacagccaa gaaaagagtt ttctaaagaa
    57841 gaattagatg aattggctga gtctgtcagg gaaaagggta tattacaacc tattctggta
    57901 agaaaaactg gcgatgtata tacaatagta gcaggggaaa gaagatttcg agctgcaaag
    57961 caggaaaaat taaaaaaagt cccagttata gtaaaagaat ttactgaaaa agaagtttta
    58021 gaaatatctt tgattgaaaa tattcaaaga aaagatttaa atccaataga gcaagcagaa
    58081 gcatataaaa gattatctga tgaatttggt gcaactcaag aagaaattgc aaaaagttta
    58141 ggcaaaagca ggcctgtggt aacaaataag ctaagactgc taactcttag tgagaggatt
    58201 ctaagttatt taagaagagg gaagttaaca gaaggacatg caagactatt actttccata
    58261 aatgatgatt taagagaaga tattgctcag gaagcagctg aaaaaggctt aagtgttaga
    58321 gaacttgaaa aacttattaa gaaagcacaa actgagccaa gaaaaaagga agaaatagag
    58381 aacgaagaag agtttataaa ggttgagaac agattgcaga aaaaactagc aacaaaagtt
    58441 tctatatcaa gaagtattaa gaaagggaaa ataacgatag aatattattc gcttgaagga
    58501 ttaaacagga ttattgatat attgagcaaa taatgtttca cgtgaaacat aattatggag
    58561 tacttatatt acggattaat aagtttagca gtttcggcag ttgtatctta tttaattata
    58621 caaatttttc tttataataa aaaaaggaga gacctaaaga aagaaataaa gcttgatgat
    58681 attaaaacat atgaagatat gctttatcag ataaataaac tatataaaaa ggatcatgaa
    58741 atagaaagta aaattagatt tataaataat cgttgtgaca agcttaaaga aaatgcaaca
    58801 tatgctataa agaatatagg tcttgtaaga tataagtatt ctgatgatgc tggcggaaac
    58861 ttaagttttt ctcttgcatt aatggatgat aatcagaatg gtctggtttt taccaatatt
    58921 tcattaattg atagtaattc gctttatgtt agaagaatta gagagggaat ttctgatgtg
    58981 ttattaatgg aagaggaaaa agaggcaatt aagcaggcaa ttgagaaagt tgagcaaaat
    59041 taacaattat tgttaattaa aaaaagacta ttgacacaat tatttaactt tggtatattc
    59101 agtaagcgcg tttacgccac gcactttgaa aaattcatac ggagagatgg tcgagttggt
    59161 ttaaggcacc ggtcttgaaa accggcgtag gggtgaccct acctagggtt cgaatcccta
    59221 tctctccgcc acacggagaa gtacccaagt aggctcaagg gggcggtttg ctaaatcgtt
    59281 agtaggggta accctagcat gggttcgaat cccatcttct ccgccaaagc agctaattag
    59341 ctgcttttta atttattcaa tctaaaaaca atagtaacca atataagtga tgataataaa
    59401 tagatataat acgaaatagt aattgtaaaa gtatctgaat ttatggtagc ataatcacat
    59461 aaaagagatt atgtactaat aaaacaaggg gtagtcatta tgtatgaggt tattgtaata
    59521 ggagcaggac tatctggtct cacagctgga gcattgttat gcaaaaaagg tataaaaaca
    59581 gcagttatag ataaaaacta tatgcctggt ggctcttgtg gaatatttaa aagagaaggc
    59641 gttacttttg atcagggatc tgccatgctt ttcggttttg gagaaaaagg atttaatgct
    59701 catagattcg tatttaactg tttagaacaa ccaatagata tgataaaaca cgatcttttg
    59761 tatgttatga acttcaatgg acataaaatt agattttatg ctgaccttga tttatttaaa
    59821 gaggaacttt ccagtgtttt tccagatcaa aaagacaata ttgtacgttt ctatgatgat
    59881 atgagtacga tgtatcagca tgttttagtt gaaacaccaa gttatactac gccagacgaa
    59941 aatgatctag atgatgcaaa ggagggcttc aaaaaacacc ccttatcata tatcaagttt
    60001 ctaagttatt taaacataag tgctgaaaag cttttaaaaa gatattttga caatgatgaa
    60061 ataattaaat tttttgataa gcttacttct acttactgct atactactac gaaagaatca
    60121 ccagcaatat tagcgtcagt aatgtttgtt gataaccaca caggaggaag ttattatcca
    60181 gcaggatcaa cactcttttt acctggatta ctagaaaaat ctattgaaga aaacggcgga
    60241 gatatgattc ttgaaaagga agtaaccaaa attttgtttg aaaacaataa gccagttggt
    60301 gttgagctaa atacaaagga aaagatacat gctgataaca taattttttc tggtactgta
    60361 tgggatttat atggaaaaat tatagatagt aatgaaacaa cagaaaaaaa gagaagaggg
    60421 gctagaaaca tgattcctac atatccatca gttgttctat attgcttagt taaagatgat
    60481 tgcataccaa aagacacaca accagtggaa atgcttgtag gaaatgttga ccaaatagat
    60541 gaaagtgaag taacagcata tatttttagt atcgatgata aaacattatg tgaagagggt
    60601 taccatgtaa tagcagcaat tggacctact tttgtaaaat ggtcagagct taatgaagaa
    60661 gaatatataa caagaaaaga gcaggaaaaa gaaagactga taaaggttct tgaaagaaga
    60721 tttcctgata ttcgaaattc tcttgtttat actaatttag caacatccaa aactcttgaa
    60781 agatatttaa ataaaaaaga tggagcagta gcaggaccaa agcaaaaact tggacagcat
    60841 atgtttaaaa gacagcacac aaaaagccaa tgggataacc tttattgctg tggcgaatca
    60901 actgttatgg gaacaggaac tcctactgtc acgacatctg gtatttcagc tgcaaatgca
    60961 atactcaaaa aattagggaa agacaagttt aaatatgata taaatattgt aaattatgtt
    61021 aatgttattg gtaaaccagt aaaaaatgat gatttgtttt ataagtatga tgatgaaaca
    61081 gcaaaaatag taaaaaaagc atttgcatgc cagctatgtg agaatccaac atgcgcagaa
    61141 cctaaagagt ttgatgtaag gggtattatg agaagggtat gtgttggaaa ctttattgga
    61201 gccaagaaac ttattaatga ggaaatatca gttgaaaaac taaaacaata tgaaaagatg
    61261 tgtattgaaa agaaaagaat aaacaaatct gtggaaatta gtgatgttat agaatttttg
    61321 aggaaataaa atgaataata aaaattatga tgtaatagtt gtaggtggag gaatagcagg
    61381 gataacagca acagcttatc tttcaaaata caacattaaa acagttttat ttgaaaaaaa
    61441 tgaaaaagta ggcgggttaa taaaaacatt tgagtataaa ggatttaaat atgatggtgg
    61501 agccagagct tatgaaaatt ctggtgtgat ttatccaatg ttgaaacaat taggaatagt
    61561 tctggaaaat gtacataatc cagtaagtgt cggtattgga gataatatta tagaattcaa
    61621 agacaaaaac agtcttaagg attatcagga ccttttgagt aataattatc cagacaatgt
    61681 tgatgaaata catgagatta ttaaagaaat cgaaaaagta attgactata tggaagttat
    61741 atatggaata gataatccga tgttttcaga taatttcaca gacaaggagt atgtcttcaa
    61801 acaacttctt ccttggctta tgaagtatac tgttaatata aaaaaagctc agaaattaaa
    61861 tgaaccagtt aacgaatact tgcttaaatt tacgaaaaac caatctttaa tagatgcaat
    61921 aacacagcat ttctttaaag gaaccccaac cttcttcgca ttaagctatt ttgggcagta
    61981 tctggattat tcatatccca taggcggaac aggcatattg atagataaat tgtcagagta
    62041 tataaagagc aaaaacaatg agattttaac agaaaacgaa attgtatctg ttaatacaat
    62101 tagtaaaaca gttaccacaa aagataataa agaatatagc tacaagtatt taatatgggc
    62161 tgctgactta agaagtcttt atctaaatgc taaaattgat aatttgaaaa aaagtgaaat
    62221 aaaaaagatt gagaaaataa aaaacaaggt cttgtctaat aaaggcagtg actcagtact
    62281 ctctgttttt attaatactg atttagaaaa tgaatactat agaaaaataa tagaagggca
    62341 ctgtttctat acccctgata aaagaggaat tggaacatca atgatggata aaagagatgt
    62401 ttttcttaaa gataagtcag aaaaagatga attcgagacc ttaaaagaga tagtatacga
    62461 atatttaaag cttacaacat atgaaatttc cataccttca ttacgagatg cttcaatgtc
    62521 accaaaaaac aaaacaggta ttattgtaag tacccttata gactacggtc tgttcaatta
    62581 tgctaaagaa aaaggatggt atgaagatct aaaagaatac tgtcagaagt gtgtaattga
    62641 tattcttaat gaaagtatat ttgacgggtt taaaacaaaa gttacagaag caacatgttc
    62701 taccccaatt acaatagaga aagtgacaaa tggttttgaa ggatccataa caggctgggc
    62761 ttttagtaat aatatgccag ttcaaagcga attaacaaag atagcaaaag ctatttatac
    62821 agagattcca aatgtgtata aagcaggaca atggtctttt tatccttctg gtattccaac
    62881 atgtattatt acaggtaaac ttgcagctga taatataaaa aagaagatga ggaaataata
    62941 tgagatataa tattgtgctt atatcggtgg gtctgattct aagtataatc ctgtacttta
    63001 gatttccgtt attagcaaaa aaagagaata ttgaaggcaa atataaagta tctgtaataa
    63061 tacctgcaag aaatgaagaa aaaacattac cgtttctatt atcagaccta aataatacag
    63121 atatgatatt gcacgaagtt atatgcgttg atgattcttc tactgataac acaaaacagg
    63181 tcatacttga taataactgt atacttgttg aagtaaatga taaaccagac aattggcttg
    63241 gtaaatcctg ggcttgtcag aagggtgcag atagagcaac tggagatgtt tttctgtttc
    63301 tcgatgcaga tgttagaata aacaaacaag gtataacaaa attaatatcg caatatgaca
    63361 aggataaatc agttatttct gtgcaaccat atcataaaat ggaaaaaata tatgaacagc
    63421 tttctttctt ttttaatata gtagcaatag gtggaaatgg agcagcttat gcttttaaag
    63481 atggatctgc aggacttttt ggtccagtaa tactaataag taaaaaagat tatattgctt
    63541 caggaggaca ccatggagca aaagaattta ttcttgaaga tttagctata ggtcaggttt
    63601 tgagggcaag gggaattaaa tacaatctgt acattggaaa aaaggatatt tcttttagga
    63661 tgtacagtca gaatatactg cagattattg aaggatggac aaaaaattat gcagcaggcg
    63721 cttctatggc aaaacctgct gtttttttaa tgacattttt atggattaca tctttatgtg
    63781 cgacagctgt aaactttata acctcatggg ttacttttga acctgtcaaa ataataattt
    63841 ttacaacttt ctacattatc tgggtttttg agttaatacg tatatcaaaa aaaataggta
    63901 atttcagtgt tttaacctgc atattatatc cagtattcat gttattcttt gtgatggtat
    63961 tcttattttc tgtatttaag aaaatattta aaataagagt tacatggaaa ggaaggaaaa
    64021 tataatgcgt ctattaatat tgcctgatat atggacaatt cttttatgta ttcccttatg
    64081 gtttattttt cacaccttat cagcgtatat ttgcttaaag atacctgata aatatcttaa
    64141 tccagatggt tttcttttta aaacaaggaa gtgggaaaat aacggattaa tatatgataa
    64201 ggtttttaaa gttagaaaat ggaaaaagta tcttcccgat gggggagcaa tgtttaaaaa
    64261 cggctacagc aagagatatc ttaaagacta ttcagaagca tcattgaaga agtttgtggt
    64321 agagacttgt agagctgaat taacgcattt attagcaatt cttccgttct ggattttcgg
    64381 atttatagct ccaccaataa ttatcttata tatgcttatt tatgctttag ctgcaaatct
    64441 tccatgtata attacacaaa gatataacag accaagaata ttatcattta taaataagat
    64501 taacgaaaaa aacacatagc actatagtat aatttattat gaataaatta ttaaaggcag
    64561 atatatgagt tttaattcac taccttattt aatattcatt atatgcgtat gtttgttgca
    64621 ttatctggtg agacctaaat acaggaatac cattcttctt gctgcaagtt attttttcta
    64681 tgtttacatt gatcctagat attcagtatt tctaatattt tctactctta tgagttatat
    64741 gtttgcaaga ttgatacaaa aggatattca aaaaaggaaa aaattctggt ttatcatttc
    64801 tatattagtt agcataggat tactagttat agttaaatat ttaggatttt ttacttcaag
    64861 tatttcaagc ctacttgcaa atttacaact gcctgttctt cctaatttaa atatcattgt
    64921 accaattgga atatcttttt atagttttac tgttacagga tatcttattg atgtatatag
    64981 aaataaggtg acagcagaaa aaaatataat agattacgct ttattcgttt cattctttcc
    65041 acaattactc tcaggtccca tagaaagagc atcaaatatg ctatttcaat ataaaagaac
    65101 aagaaaattc agttttgaga atctcaaaac aggaatgata aggtttctat ggggattatt
    65161 taaaaaaatg attatagcag accagttagc tataatagtt aataacacat attcggtgtt
    65221 acctaatagc tcttcattaa atgttattat tgcaattttt gctttttcat tacaaatcta
    65281 tatagatttt tctgcatatt cagatatggc tttagggtca gccaaacttc taggtttaga
    65341 tataatggaa aactttgata gcccgtatct atcatcatca gtaagaaact tctggagaag
    65401 atggcatata tctcttacgt cgtggtttaa agattattta tatattcctt tgggtggaag
    65461 ccgttgttca aatataaggc atttgttaaa tatatttatt gtatttgcaa taagtggatt
    65521 atggcatgga gcatcgttca cttttcttat atggggaata ataaacggga tttatttgat
    65581 tattggacaa gttttatctc ctgtttattc aaaaattaaa gaaaaggtta aaattaatga
    65641 aaacaacaga ctatttcttt taataaaagg ggtatttact ttcatgttaa taagctttag
    65701 ctggatattc tttagatcag aggatattga acaggctttc ttggtcataa aaaaaggatt
    65761 tactagttca cagcctcttt taaattatca gcagataggt attagcctta gcaatatgat
    65821 tgttttgggt gcatctttac ttcttctgtc atatgttgat gtctttacaa gaaaaaatga
    65881 ccttatttct gagataaata aaaaactatg gcttacctat gcaatatgcc ttgctttgat
    65941 tatttccgta cttatatttg gtgtttatgg gcctggatat gatcctatag actttgtata
    66001 tttcaaattt taaccaaagc ttataatgtt atatatgtct aacattataa gcgttaagtt
    66061 tacaaaagca taaaagctga aacttgcatt tgtataattg tgaatacagg aactattgat
    66121 ataataattg tggtatttta aattgcaata tgctacatgt agtggtgtca tataaatgaa
    66181 atatatttat tgcaacaaat atttaaaaaa cttgacatac cttattataa atatatataa
    66241 tctaatgcgc tatgcatgtt ttcatgtgag cacaggaaat gaaaaaaatt tatttatata
    66301 caggagttac ttatgaaaag aacgtatcag cctaaaacaa aacagagaag caaagagcat
    66361 ggattcagaa aaagaatgag ttctgctgct ggaagaaaaa tattgaaaaa tagaagagac
    66421 agaggcagaa aaagattatc tgcttagtta atccttttag ctgtctttat tatgaaagga
    66481 aaaatatgga gacgctgaaa aaaaactatg aattttcacg tgtatacaga aaaggaaagt
    66541 acttcagcgg acaatatttg actatcaatg tacttgaaaa catatatggt tatagcagga
    66601 taggtatatc tgttagtaaa aaagcatata aaagcagcgt taagcgcaac ctctatcgta
    66661 gaaggatcaa agaaatagta agaacaggtt gcttcaatct aaagcaaggg tatgacttga
    66721 tatttgtagt taagaagaca gaacaaatac caccataccg tgaaatagaa aaggaaataa
    66781 tcgatttgct ggaaaaggct aaattgatat taaagaatga agaaaataat aatcaagttg
    66841 attaaatttt acagaatcaa tatctccccc aacaaactgc catgctgcag atttgttcct
    66901 acttgttcgc aatatgctct tgaagcagtg gaaaagcatg gagcactaaa aggatctctt
    66961 atggcgattt ggaggatatt aaggtgcaat ccgttatgta aaggtggata tgatcctgtt
    67021 aaatagggaa aggaaaacct atgtttgatt ttatctacaa tttgttcggt aagttttttg
    67081 aattgctcta cactggatta tgcgcagaga attttggtgt aacacttata atttacactt
    67141 taattcttaa gcttttgctc atgccgcttt ccattaaggg tacaaagtcc accatgcgaa
    67201 tgagtgaact gactcctaaa gtagatgcta taaagaaaag atacaagaat gaccagcaaa
    67261 aacagaatga agaaattcaa aagctataca aacaggaaaa tgtaggatgt gcatcaggat
    67321 gtcttccaac acttatacag tttcctttgt taatagcaat gtacagggtt ttgtcgcaac
    67381 cattaactta cattaaaggt gtatccaaag aagttattac tcaaattgct gaacgtttag
    67441 gatatggtgc gacagtatta cagacaacaa taaataatga tcttattaca gatcctgcta
    67501 aaatggaact tgtaaaagat cttattactc cggataagct aatagatatg aaatttttag
    67561 ggatgaatct tggacttgtt ccaaaaatta atccttcaat actctttggt gctgaaacta
    67621 taggtgcata tcttcctata ctattccttc ctttgatttc agtaggctta gttattcttt
    67681 cacagtatct gacagaaaag gcaatgaaaa gcagaatgcc tannnnnnnn nnnnnnnnnn
    67741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    67801 nnnnnnnnnn nnnnnnnnnn nngttggaga tgaaccaaac agaaaaatag ttataacttt
    67861 cgaaagaaac gggaaaagaa gcagttatta attaaaaatg gaacatacta tatctgcaat
    67921 atccacacct ataggcgttg gaggaatatc cgtaataagg attagcggcc acaatgcatt
    67981 ttcagtcgct aaaggtatgt tcaaatcaaa aacaccattt gataagataa aaagtcatac
    68041 agttacatat ggaaaaattc atgataataa agggaacaca ttggataatg tgctccttct
    68101 taaaatgaaa gctccaaaga catataccaa ggaggacact gttgagatac actgtcatgg
    68161 cggtatatac ataacgaaaa agatattaga acttacatac gaaaatggcg ctttaccggc
    68221 tcaagcaggg gaatttacta aaagagcatt tattaatggt agcatagatc ttatggaagc
    68281 agaagcagta atggatttaa taaatgcaga agctgaagat gcatacaaag atgcacttgc
    68341 tcagttaaaa ggtgaaaaaa gcatagaaat taaaatgata agaaatgagc ttattgacct
    68401 tatttctcat attatggtta tcatggatta tcctgaatat gatattgaaa aactgactgt
    68461 tgatcaggca aaaaagcaag catgtattat aaaaaaacaa ttaaaagaat taatagagga
    68521 ttttgataac agaagaataa tcagagaagg actcaatgta gctgtagtag gagatgtaaa
    68581 cgtaggtaaa tccacgttta taaatcgtgc atgcggacaa aatagggtca tagttacaga
    68641 tattgctgga acaacgcgtg atgttgtaga ggtcaagatt gatgttaaag gattatgtgt
    68701 aacactgaag gataccgcag gtattcgaac aacagaagat gaaatagaga aaataggaat
    68761 tgaaaaatca aaagaagcaa ttgagcaggc agatgctgtt atttatattg ctgatataaa
    68821 cagacctaag aaggatgaga tatttaacta tatcaagaat cttgataaaa ctatatgcgt
    68881 tcttaataaa accgacctta taactgaaaa tgaaagagaa cagcatatca gtaatatcga
    68941 aaaagactta cagaagacat ttaaagacag aataatatcc tgttcattag acaaaaaaga
    69001 aggaataaat gaaatatttg ataaacttct agatatggtt gatgcaggca gcataagaca
    69061 gggaacatct ataataacaa atgccagaca tagaaagcat attctagatt cgatagagtt
    69121 tctggaaaat gttgagcagg gttcatatat agacatgctt agtgttgatc ttacttgtgc
    69181 agctgatgaa ctgggtaaaa taacaggaga aacagcaagt tcagatattg tggacagaat
    69241 atttcaaaaa ttctgtgtag gtaaatagta tgataaatta tgatgtaatt gtagttggag
    69301 caggacatgc aggttgtgaa gcagctcttg caagtgcaag aatgggtatg aaaactgcta
    69361 tctttacctt aaacctggat agtatagcaa atatgccatg taatccaagt atcggaggca
    69421 cagcaaaagg acaccttgta agagagattg atgctttagg cggtgaaatg ggaaaatgtg
    69481 cagatgctac tcttatacag tcaaaaatgc taaatcgttc cagtggacca gcagtatttt
    69541 cactaagagc tcaaattgat agaagacaat atcagataaa gatgaagcat atacttgaaa
    69601 tgcaggaaaa ccttgatata aggcaatatg aggttgttga tgttgttttt gaaaataaca
    69661 aggtaaaggc agttaagaca aatacatcag cagtttttgg agcaaaagca gtaattatag
    69721 caagcggaac atatctcaaa gcaaggataa taattggtga aacatcatat gagggaggtc
    69781 ctgacggtct gtttcctgct aatcttttat ctgtaaactt agttgaaaag ggtatgaagt
    69841 taaaaagatt taaaacaggt actcctgcaa gagtgcataa aagcagtata gattttgaaa
    69901 aaatgtccat acagaacggg gacgagccaa tagttccatt ttcatttgaa acagagagta
    69961 tagatataaa tcaggaacct tgttatctta catataccaa caagaataca catgaggtta
    70021 ttagaaataa tcttcatagg tcacctcttt tcagtgggca gataaacgga acaggagtaa
    70081 gatactgtcc ttcaatagaa gataaaatag ttaaatttgc agataaagaa aggcatcagg
    70141 tttttataga gcccatgggg ctggatactg aagaaatgta tgttcagggt atgtcaagct
    70201 caatgcctga ggatgtacag gaagagatgt ataaaacaat aaaaggattg gagaatgtca
    70261 aattcatgag aagtgcatat gctattgaat atgactgttt tgatcctttg cagatgcttg
    70321 caacattgga gtttaaagat tatgaagggt tttattctgc cggacagagt aatggaagca
    70381 gcggatatga agaagctgct gcacagggaa taatggctgg gataaatgct gctttgaaga
    70441 taaaaggaat gcctgaactt gttcttgaca gatctgaagc ctatatagga gtattaattg
    70501 atgaccttgt tacaaagggt acgaatgaac catacaggat gatgacaagc cggtcagaat
    70561 accgccttat actaagacaa gacaatgcag atgaacgtct tactcctaaa ggttatgaga
    70621 taggattaat atctaaagaa agatatgata aattccttac taagatggat aatatagaaa
    70681 gagaaataga aaggcttaag aataagaatg tttctccgaa aattgccaat cctttctttg
    70741 aaaaaagaaa tcagcagact ataagttcag gaataacaca aagtgcaatg ctaaaaagaa
    70801 atggtataag ttatacagac ttttgcgaga tagacgatga aatgcctcct ttatctaatg
    70861 aagaaaaaga agaagtagag ataaaaataa agtatgaagg atatattgaa aaacagttaa
    70921 gacaggttga acagtttaag aaaatggaaa gaatccatct ttcaagagat attgattata
    70981 gcaagattac aggactaaga aatgaagcaa aacaaaagtt gaacagcatg aaacctgcat
    71041 cattagggat ggcaggaaga atatccggag tatcacctgc tgatatacag gttctttatg
    71101 tatatcttga acagcaaaaa agaaagatga aagagagtat tgatgaagca tcttcttaaa
    71161 ggaatacttg actatgctgg attagagtat aaagaaacaa ttgatataat gttcagttca
    71221 tatgcagata tgcttattga atggaataaa aaatttaatc ttacaggaat aactgattta
    71281 aatgacatat atattaagca ctttgcagat agccttatac tttacaaagg aataaagtca
    71341 gcttcttcgc ttatagatat cggaagcgga gcaggatttc ccggaatacc attgaaaata
    71401 gctgggtatg ataagaaagt agtgcttctt gaagcaaatg ggaaaaaaac aatgtttctt
    71461 gaccatgtaa ttaacagtct taagttaaaa gatatatcta catgtctttc aagagcagaa
    71521 gttgcaggaa gggattcttc tttaagagat tcttttgaga ttgctactgc aagagctgta
    71581 gcaccactca atgtcttgtt agagtactgt actcctttta ttaagaaagg cggagtattc
    71641 attgccatga agactgatga gtcagaatta aacatagcaa agaacgcaca aaagcagtta
    71701 ggtgtggaag tatgtgatat tgatatgcag attttacctt tttcagatat gaaaagatgt
    71761 aatatatatt ttaagaagat caaaaatact ccagataggt atccaagagc agacgggata
    71821 cccaaaaaga agcctcttta gaaatattca caaatattta attattagtt cacagattaa
    71881 tcacatatga gccttattat gaacttagtt aatttcataa caacattcct catagaaata
    71941 aagaaagacc tagtctttct tttttttcat aacatgaatt ttgcatttga ttaaaaaagc
    72001 aggaatacta agcattcttt taaatctttt tggttgtttg gcaagcctgt ataaccattc
    72061 aagtttgtat ttgcagaaaa actcaggagc tcttttagca tatcctgaga ggatatctat
    72121 tgttccaccg actcctattg caagaccaga tttaatacga tttctgttat catatatgaa
    72181 gaactcctgc tgtggggctc ctagaccgat aataatcaca tcaggctgtg ctgtattgat
    72241 atcatcaata atcccttcca catcatcttt aacaaagtat ccatttctat aacctgaaat
    72301 gttaatatga ggatattttt ttaatatatt ttcttttgcc ttaattacag actcctcaga
    72361 tgcgcctaaa aggtaaactg aaaaagattt ctttaaattt aaaagatttt gaacaaatac
    72421 acagccagga accttttctt ttaagggtgt tttcagtatc ttagatgcta gtataacgcc
    72481 ttctccatct ggaatattca tatatgagga atttaatgca gtcataagtc ttttatcttt
    72541 taatgcagcc atacatatct ctgcatttgg tgtaaatatg tatttgaaat tatctttaat
    72601 aattatagcg tcataagctt ttttcaaagc ctcttccata tcaatatcat ctattcgtaa
    72661 acctaaaata tccatattgt ctccaataca ttgtagtata ggcaaaaaac tgattaaaat
    72721 caataaaaag gttgctattt aaagtattta gatttagaat aaatctcgtg aagatgaata
    72781 agaacggggc agtaaaaata attctaataa tattaataat gtcagcacta cttacttctt
    72841 gtacagaaga agagtaccgt gcttcaaagc tttatcgttc tttgacctca tacgagcagg
    72901 ttggggaaac agttacttta aannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    72961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    73021 nnagctttat cgttctttga cctcatacga gcaggttggg gaaacagtta ctttaaagaa
    73081 taagaaatat aacagagtat atttaacaga agaagcgcag ctggatatgt caaaagttga
    73141 gatttataat gcggacttcg atcaggctgt tgtggtaaag gattcatcat cattagcaat
    73201 cttgaattcg cagatacttt caaaaaatac agccattaca tctgtaggag aagaaacaat
    73261 aataaatgcg atttcttcag agatggcttc atatggattg tttgttcttg atatttcaga
    73321 caaagctctt ttcacagggt caaatttaaa aataacaaat ttaagtgaaa atggttctgg
    73381 cataaatgta agaagcgaag cttcaatggt tattaataac tcaaatgtca gtgtaaaagg
    73441 agttggtgtt gaaacagatg ctattgtaag tataagtaat tctgaaatta cttcatcaag
    73501 tttaaaattt tatgaaggtg catcagttac attgacagat tcaagattct acacagacca
    73561 tggactgctt cttgttgata acccgacaga aaacaaaatc catgtttcat taaatttaga
    73621 aaaagtaaag cttaatacta catatggagc agcattatcc atgatagatg caaaagcttc
    73681 agttaaaatt tccgaatcaa caatagggca gaactcaaaa aatgtaatgt ctttatctaa
    73741 ttcagaaggt acacttactc tatgtaatac atctattgaa ggtaacatta atacagatga
    73801 aacctcatca ttgaatatcc ttttaaaaca ggaatctgtg cttattggac agattaataa
    73861 tgaaaataaa tacaacacag taacaataca gattgaagaa gcaagtgtct gggaattaac
    73921 acaagacagt tatgtaagag gcataatatt aaaggataag acacttaatg gaattaaatc
    73981 aaatggacat gcaatttatt atgatacaaa atgtgcgaca aacacatggt tgaatggtaa
    74041 tactatttat ttagaagacg gtggaaagat aacacctgat aactaagcac agttacaagg
    74101 ataaaatatt ttttgcatta ttcatatagg tattaaattt cgagccaatc atattatggc
    74161 tcttttttaa atacttatat gcaaagaaat aattataaat atgactatca ctggcgataa
    74221 aagttaccac attactcttt atatatttcc ttattctaaa ccatttaata ggtgaaaaaa
    74281 gtgaatatgc attaatttga gttaaaacat caaactccat taatttatta atctgatttg
    74341 agctatatct ttctatgtgt gcaattatga tagtaagacc caaagactgc agtttaataa
    74401 tttcatttaa taaaatatta ttccaggtag ttgaagaagg catttctaaa agaagatagt
    74461 tcgtgttctc gaaagcaagt tttttaatat gcttaaggct gctcaatcca gttgtcaatc
    74521 gtacttctgc gcctttatat atctttattc cggtactatt tgcagcagat aactcatcaa
    74581 atgctttgtc tcttcttttt aagaattttt caatactatc attaaaggga taaaaatgag
    74641 gtgtagcaac aatgccttta acaccatatt tagaagcagt cattaactgc tttaaagatt
    74701 cttctaaatt atgtgatcca tgatctgctt taggcaatat atgggaatgt atatcaatca
    74761 tcatcaagac ttactggaga tgccgatttg acaggtttct tatcatatcc tccatatctt
    74821 tcatacttac catatttgta ctgtttatta tatgccatag actcaatatc ataatcatta
    74881 aagataaaac ctaggacttt accgtttacg ttattgaggg catcaacagc ttcttttata
    74941 agattggttc gagaaaaatt ctgtctgaca gctacaactg tgccagttac tttgtttgat
    75001 aaagcaatag catcagttac tataagaata gggggagtat caataaagat gtagtccaat
    75061 gtttctctaa gctcgtttaa tagtattgac attctttcag atgttaataa ttctgatgga
    75121 ttaggaggaa ttgttccgct aggaagaaca tgtaggtttt tatactttgt cgggtaaata
    75181 aaattttcct ttacaagccc gcccagtata tcgcttaaac caggggaaga tttaatttta
    75241 aaatacttat ttaatgttgg tttgcgcata tccgcatcaa ttactagcgt tttaaatcca
    75301 gcttctgcaa aagcaagggc catgttggcg caaacggtag attttccctc gcccatatgt
    75361 gcgcttgtaa aagaaataac aggacacttt tcctttgccg tagtaaacat taaattagtt
    75421 ctggttgctt tgatggcttc tgttacggga aaagaactat tatcaaacag gagaaactca
    75481 cgcctttcta cgtctgccat aagagtggac ctccttatca tctttttgat ataactgaag
    75541 atcggggata tatcctataa caggtgcttt aacaacacgt aataggttct cctcgtttct
    75601 tactttagta ttaaacaatt catatagaat aacaaatgca actgccgcta taacacctaa
    75661 cactgcacct attgcaatat ttttcatcat acttggtgaa gaaggctgtt gcggtatttc
    75721 agctctgtct aatatttcaa cagcaccagc tttgattact cttagtatct catctggtgc
    75781 gacatcaatg aacgcatttg caagaaccat tgaatcaaca ggatctgcac ttgtgattgt
    75841 taccttcata acctcagtat tatttacaga ggtgccagta aacatatttc gtatctggga
    75901 aggtgtataa cgcagattag ttgtttcaat taccttatga ataacattat cgctttgcat
    75961 aataacagaa tatgtggcga taagtttttg agacgttgta atatctgttg tggaaattga
    76021 agtatctgac cggtttgaat cattaaatac gtataatgtt gctgatgcct catactgagg
    76081 agttatgaaa aagtatgtta tagttgctga aaggcttgct gctaccaaaa ctatacaaat
    76141 tatcagccaa aagtggcgtt taagataagt aaatatttgt ctgacatcta attcttccat
    76201 aaaccctctc ttttatataa aattcctaaa tagtgaaatt aataatagaa tatgtgcttt
    76261 taaatgtcaa ggcagttaga aattataaat ctcactttta tttatatatt aaacatattt
    76321 ctactcaata attattattc cataaatggt aatattattg tttttacata tgttaataat
    76381 tttctcaata tccttaactc ttgaaacatt acatttttca acaagtatta tacccttaca
    76441 atcaagaagt gttttagcga tatctgaaga gttaaaaata ttctcaaggt gcataaggtt
    76501 atattccgtc tgttctttta agctgctgaa tattcttttg gcatgagata tgttacctga
    76561 tgaaagaact cctatgtgtt cttctgtttt accacaggcg taaataacag gtactacaca
    76621 tgctttttcc agtttttcga tatcctttcc acttgccaat aaagatattt tagccattaa
    76681 ctgttctata cctaaaaact tagggcttaa atacaatctt cccaatataa atacataggc
    76741 aaggaaaact cccaagatca aacctaaaag ggtatattga aaaacatctt tgctaaaaag
    76801 agtaaactgt atttttgata atgtatcatt agtctcagtt atttgtgtat ctatttctcc
    76861 aatagaggta ttgattgttg aaatacggtc ggtatatgat ttctgaagat tggctattgc
    76921 agaatttgat gcaaaagatt cattttctga taacagatgg attgtacttg aacctaatgt
    76981 tattctgata gtatcttttg catgactaaa gcactgttca gctatatctt ttaactttga
    77041 taactgttct tcatttttag cagatacatg tattgttaat gacctatatg atgaatttgc
    77101 agtgaaatca attagcatat caacaacatt aaaagataac tcaatattac tatttgagta
    77161 agctttttca ataacattta tatcattgat taccgtatta tacatatcaa catttctatc
    77221 aatataatat acagcagtat acttcaataa tcttgaagca ttcatatctt taacagatat
    77281 atattcttcg ttattatcaa ttttatcaat atcagaatgt aatgcatttt tttgtgcctc
    77341 taaactgtct aattttgcct gaagctgtgg aatagtctga ctgtcttttg agtatgttac
    77401 atagtcagtt aacattttta ctccgcctgc aagcaatgca caacataaaa ccacgactac
    77461 aatcagctta aggttcttgc gtgaatattt taaaagtgtg gataaatcca tatctcctcc
    77521 tctagaagaa atgatttagt tatttacaat agaaaaatta tatcactttt attctatttt
    77581 aaccagtaga aatagggata ttgcctctgc aaatatgaaa tgacagaagg tggtataata
    77641 tgaggacacc atatgtgttc aaatgctata tataagacct attaacgatt gaaaaaaagc
    77701 cttatgtaat atataattca tatgttaata aataaaggga tatatgtatg agtaacaaga
    77761 aggcgaaaaa aaacgttgaa gaacccaaaa caaagaaaaa tcttctgaaa gattttttct
    77821 atctagagca ggacaaaaag aaaccaaatt acttcgaact gataacaaca gcatttttgt
    77881 atgttttcct ttccagcttt gcattaataa taagcagaaa agtatatggg gatatgacag
    77941 agacgaagct ttatacgctt attgctatgt tcagtatatg gctgattata atgattttcg
    78001 gatttgttga gaaaatacgc tcaaaaaaag caaataccct ctatttcaaa caggccgcta
    78061 agaatctttc tgtaacagat atagctttaa tagcatacat gcttatagcc tttatatctg
    78121 cattgatatc agaatacaga catgtagcat ttgtcggagt tgaagcaaga aacgaaggat
    78181 atctgatgca acttatgtat attgccattt tctttatgat ttcaaggtca tatatacata
    78241 aactggaact taaaattttc gcacttgcta gtcttcctgt agctctattg ggaattttac
    78301 agcttaacgg gtattatgtt ggaagaataa catatgatat tacaactcag ggaacaagct
    78361 ttttaactac tttaggtaat atagacgtag tttccacgta tcttgtaaca cctataatgt
    78421 tgtttactgt tctttattgt cagagcaaag aaaaattctg gcgtatactt tatcttgcat
    78481 cagctgcaat atgcttctat ttttccatta tgataggagt tgacagcggt tacgtaggaa
    78541 tggcagctgc aatattcttt atgtttccct ttattgctaa agacagaata tctgcaagca
    78601 gaatgtttca gattctttca gttttccttt ttgttattta tctggaagat caaattgctc
    78661 agataggaaa taggaatttc acattgttta gcaaaccttt ctctgaaatc atgtttctag
    78721 cggcggttgc gtgcttagtg acttctgcaa taattttctt tattggcaga tacattaaga
    78781 ttgaaaagaa atttatatgg cccgtaacat actttgttat tgcatttgtg gcagctttta
    78841 ttgttataaa aaagataatg gctatggaag aacctcaagg atcatcagta atgctggttc
    78901 tgtatcagtt cgggcagata cttaatggaa atattgatga tagtttcggt tcaaacagaa
    78961 tttatgcatg gcgtgtagtg tggggtttct ttaaggaaaa acctatactt ggccatggtc
    79021 cagatacagt gtttgattta tggctaagag aagaatatca gtacagcatg aaagagttca
    79081 atgtatattt tgataagtca cataatgatt atcttcaggt gttagtaact acaggattgc
    79141 ttggatttat atcatatctg acattccagt tttcactttt agtaaggtcc tttaagaaat
    79201 ttaatcagcc tttagttata gcattggtta ctgcaagtat atgtttctgt gttcaggcct
    79261 ttttcaatat aggggttcct attgtcgcgc catatggctg gattattttt ggaatgcttg
    79321 catcagtttt aagaaaaaaa gatgaattgg atgaacagga aatgggggtc tagatatatt
    79381 tggttaagaa gattaaggat aacagtcttg ttttaaaaaa gatattactg gttattgcag
    79441 ataccgtatc cattaatgca gcaacattct tagctctctt tttcagatat gagctgagtt
    79501 tttcaaaaat tgatttacag tatattattc atgctcagca gtatctttgg atagatacca
    79561 ttttgacttt atgtattttt gcattattcg gtctttatac cggattatgg aaatatgcat
    79621 ctatcaaaga actttttaac attctgcttg catgttcatt atcttatctg gttaaattca
    79681 cattaacatc aataataggg cttaatcagc caagaagcac tgttttcttt ggttgggtac
    79741 ttttagtaat gatggttttt gcaactcggt tcagttacag gtttatcaga tatattgttc
    79801 atgaaaattt aaatggtaaa gatataaaaa gggtaatggt aataggtgca ggggatgctg
    79861 gcaatatgat tataagggaa ataagatcaa gtcagaatct tcattccaaa gtggtatgtg
    79921 taattgatga tgataagaag gtacatggtt tattcattca gggtgttaaa attattggcg
    79981 gaagagatat gattgttgag atggctaaaa agaacaagat tgatgaaata attatagcta
    80041 ttccatcagt cggagcttct gaaagaaaga aaatatatga tatctgtaaa gaaacaaaat
    80101 gtactttaaa aacaattcct ggaatgtacc agttaattaa cgaagaggta aggctttctg
    80161 atcttagaga agtacagata gaagacttgc tcggaagaga tacgatagac ataaatgtgc
    80221 aggagatact agattatgtc agtggaaaag ttattctaat aacaggagct ggtgggtcaa
    80281 taggcagtga attatgcaga cagattgcaa tgcataatcc ttcaaagctt ataatgctag
    80341 atatttatga aaacagcata tatgatgttc aacttgaaat aacaaaaaaa tatcccgctt
    80401 taaagattga aacacttatt gctagtgtta gagatgaaaa aagaattaaa aatattatga
    80461 gcaaatataa gcctgatatc attttccatg cagcagccca taagcatgtg ccattaatgg
    80521 aaacaagccc taatgaagca gttaagaata atgtttttgg tacacttaca ctagtaaaag
    80581 ctgcagatga agcgaaagtc gacaaattta taatgatctc aactgacaaa gcagttcggc
    80641 ctacaaatat aatgggtgca actaaaagaa tatgtgaaat gattattcag gcatacgata
    80701 agaaatccag tacagatttt gtcgcagtca gatttggaaa tgtactaggc tcaaatgggt
    80761 cagttatacc aatattcaga aaacagattg cagaaggcgg tcccgttaca gttacagata
    80821 aagaaataac cagatatttt atgacaatac ctgaggcggt atcactggtt ttacaggcag
    80881 gatattatgc aaaaggcgga gagatattca tattagatat gggtgaacct gtaaagatat
    80941 atgatttagc aagaaatatg attatgcttt ctggcagaac tcctgatgaa gatataaaaa
    81001 tagaagttac tggtttaaga ccaggggaaa aactatatga agaactgctt atggacgaag
    81061 aaggcataga aaaaacacaa aacgacctta tatacatagg acatccgata caatttgatg
    81121 aaaaacaatt ttttgataac ctgaagcaac taaaaaaaat gaccgaagag gaagtatcct
    81181 gcatgaagct aataatagca aaaatgatac caacatatga acctgacata tcagattcag
    81241 aaggtataaa cagcatcgaa acagctgaat gcataacaag gcttttcgct catgataatc
    81301 aaaagatgta gttatacaaa gatataactg aatatataaa taattgaaac ggaggtattt
    81361 tgtatgaaga cagatgacaa aatccaaaaa ctcgttgata gttatggctc atggttcaac
    81421 gaggatagcg gattcaaggc tgcgaaaatg acaaacaagc atgcccctta tcccctatta
    81481 ttttctccta ttcaggttaa caagctgact attaagaaca ggcttgttat ggccccaatg
    81541 ggaaatatca atatggcgga agaaacaggc aggccgaatg aaaagatgat cgcgtatttc
    81601 actgagcgtg caaaaggagg agtaggctta ataaccactg gccttattcc gataagtttc
    81661 ggaatagaca actctttaat tgagctggat aaattaactt acttcccaag gattgaccgt
    81721 tcaaggactg tttatatggg atggagaaac ttggcacatt ccgttcattc atatggcgct
    81781 cgcatttttg ttcagttaac accaggctta ggaagggtgg gaaatccaca gtgtttaaca
    81841 aatcaattta aatttccagt atcggcttct ttgaatccta actggtatat gtcagaagtt
    81901 ccctgtatgc ctctgactga tgcagcatgc aaaaagataa ttaaaagggc aggacaagct
    81961 gcagcagatg caaaagcttt cggacttgac ggagtatatc ttcatgggca tgaaggatat
    82021 ctgcttgagc aaatgacaaa tccagcattt aacagaagaa agttaggaag atatgcaaac
    82081 tggcaggcat ttggtttgga tatgatagaa gaaataagaa gaagaacgga taatgccttc
    82141 ccaataatgt acaggataga tctttcgtta gccttaaacg agacatatgg caaaacgatg
    82201 gatataaatg cgcttaaaaa gttcaaaaat ggtagaacag tggaaatgac tttggactat
    82261 atgaaaaatc ttgtatccaa aggagtagac atgtttgatg tagatttagg ctgctacgat
    82321 aactggtggc tcccacatcc accagaagga atgcctcctg catgtttttc tgatatttcc
    82381 aggatagtaa agaaatattt tgacgagtct aatattcttt ccaatgcagg cgtaaaaatt
    82441 cctgtggtag cagtaggtaa gcttggatat ccggatgttg ctgaggaagt gcttatgcag
    82501 gaaaaagcag atatgataat gctaggaaga cctttattag cagacccgaa gtggccactc
    82561 aaagcaaaag agggaaggac agatgacatt attccatgta taggctgcca ggaaggctgt
    82621 ataaatgaat ttgtagaagg tggacatccg caatgtgcag taaatccacg tactgcattt
    82681 gagaaccttt atcctgaaga gccaaaacag gctgacaaga agaagaagat agcagtaatc
    82741 ggttcaggac ctgctggcat tatggcagcg gttacagcag ctaaacgtgg tcatgaagtt
    82801 gtaatatatg aaaaacagaa tgttattggc ggaaggctta atgcaggagg aaggccaaag
    82861 ataaagtttg acatccagaa ttatcacgaa tatctgaaga agcttattga aaggacctct
    82921 aaggaatata agctgaaatt ggtccttaaa acagaagcta atgcagaatt gctaaaaaaa
    82981 gaaggattta atgcagtaat atatgctact ggaacaaagg atgtggaatt gaagcttcca
    83041 ggatatgaaa aagcaaacaa agtacaggct gttgatgtgc tggataatcc taaaatagta
    83101 gataaggcta acaaagttgt aataataggt ggaggagtag tgggttgcga aacagcattc
    83161 ttcttaaata aggagttagg aaaagaagtt actgtgatag agatggaacc atatttcatg
    83221 aatcatgttt gtactgcaaa cagagcttat cttatccact atatggaaaa ggcagatatt
    83281 aaactactga actgtacaaa gatgctaagc tatgagataa atggagtcaa agtatcaaga
    83341 aatgtatcca agacagtgcc taatccatat aagacatgga atcctatatt accagctaac
    83401 atagaaaatc ctcttgccaa gaagataaaa gaagaatatg aagaacagtt tattgaagct
    83461 gatatagtaa ttcaggctgc tggcggaaga tgcgatgaca gcggattcta tgctttacaa
    83521 aaagaaatgt ccacatgtga gatgtataac attggagatt catttaaggg tggcaaagta
    83581 tttgaagcta ctaaatcagg atatgctgtc ggattatcag tttaaggggg ttaaaatgga
    83641 ttacaagatg aaacttactg aagatgaatt ggctatcctt aatggtgaaa aaggcgaagt
    83701 aatggcgaaa gttatgaaat ctcttgtcat gtatggagat gttttcaatg ctaaaagaat
    83761 ggttgaaata agtgctcctt cacattttgt cacaagtttc gggatatcta taatgaaagc
    83821 agtctttgac ataatggatg agataaataa tgccggaata accacgattg aaaaatttac
    83881 agttgatcca agacctctcg attatgataa tgtcaaatgc aacccattag aaaaacttgt
    83941 ttttggtatt atgtatggga aacaagagga ttacgaagag cagttgaaaa aaactgggtt
    84001 aaaagacagc aatgcattta gctgtacctg ttacatggag gaagtaggca atattcctaa
    84061 aaagggagat attctttcat gggcagaatc ttcagcagta gtttatgcaa attcggttat
    84121 tggagccaga tgtaacagga actccgcact tatagagatg tttggagcta ttctgggtaa
    84181 ggtccctgaa tttgatcttt taacagatga aggaaggaaa gcaacgtggg ttgtagaggt
    84241 taaaaccagc aaaaaacccg aagctcaggt attgggaagt gcaattggaa tgaaagtcat
    84301 gagtgatgtc ccatacataa agggactgga taaattcatt ggaaaagaac ttagcgaaca
    84361 ggcaaaagat tatctgaaag acatgggtgc tgcaacagcc tccaacggag cagttggcct
    84421 gtatcatgta gacaatctaa ctcctgaagc agtcgagttg aagcatgcct tgattcaaga
    84481 aaatgcaaaa gtatatgtta tagacgatgc agaactggaa agagtaaaaa acagctatcc
    84541 aatcatatgg aagaagcctg atgcaaaacc atcaagatgt tttatagggt gtcctcatct
    84601 ttcatataat cagcttatag agtggacaga cagaattggc agagaacttg ataaacagaa
    84661 aaaaacgaaa gttgctcttg agaccatatt tacatctgct cctgatgtgg ttgcacattt
    84721 taaagaaaac aaggaatatc acgaaaggct tctaagttat ggtgtcagga tttcatcaat
    84781 atgtccgttg atgtatatga ataatcctat ttgtgcaaaa agagctgttg taacaaactc
    84841 caataaatta agaacatata ctacagcaag gtattacacc gatgaagaaa taaccagtat
    84901 tgcagtaagg ggggatattt caaaatgaaa aaggaattta aaggaagaaa tgttgtagct
    84961 ggcacagtac aggcacaatc tgttgtttca agaaaaggga tgaacacctt agccaccttt
    85021 caaaagagca tactagcaaa aaagaaaaca gtatttggaa gcgatcagaa taatgaggat
    85081 ttgtataaaa aagagataac aggaaaagca ttatgtcttc caaaaacaat aggttcaaca
    85141 acaggcggta tggtgctgca aagcgcagca gctttaggct tagctccaaa ggcaatgctg
    85201 tttagtgaaa gtatagactc gattggtgcc gcaggagtaa tacttgctga tgtatggact
    85261 gtgcataaaa tagttacagt ggataatctg gggcaggatt ttcttgacta tgtaaaggat
    85321 gggatgactg ttacagttaa agaagacggt actgtaattg ttgagtaata ctacaaagca
    85381 tgtaatatat gtaaatggta tttgcttaat aaaaaccgga taaagcaaat attaaaaaag
    85441 actgaaaaga tagaaagaaa aagcgaaaag aacaaaataa atagagaact taagaaataa
    85501 agagtattaa taaaaagggt tatgctgcgc atagcccttt tgttaatcta atagttcttt
    85561 attaattatg gttacttctt atagaactga ggaagatatt ctttgttctt ttccatcatc
    85621 tcatcaacca tttctttaat ctgatcaagt gataaaacag cagctgtgag aggatcgaat
    85681 gcaacagcat gaaatacttt ctttgcattt ccttctaatg cagcatcaac tgccataact
    85741 tcgcaacgtg ctgttgtgtt gatcaatgtt gccaaatggt caggaatctt tcctatatgt
    85801 ataggctcaa gggagtcttt tgaagctaat accggcactt caacacagca gtcgtatgga
    85861 agattttcaa tgtttccata gtttatgaca tttccattaa atttgaacat ggtgttgtct
    85921 ccaaatactg cattgaatat gtatatagca tattcgtgcc cgcgaacaag atcaacttca
    85981 tccttatcca gccattctct tatactgtct ctccagttat ccttgcgttt catatattca
    86041 tcaagaatat atccatgatg accagggttc cagtttgtgc cgtccataca gtattttttc
    86101 aatgtttctg gatttttcct ataccaggca acatattcac tgttgtgtcc tgaagattct
    86161 gtaacataat aatcaaggtg ctcatacatg ttgtttctaa ccagctcttt tttgtagatt
    86221 tcaggatctt tcagtgcctc ttttattaat ggatatgcat cttttccatt ccatttgtat
    86281 tcaagataga aggcttgatg atttactcct gcacatttgt aggttatttc atctgctggt
    86341 gctttaatcc agtctgccaa catagatgct gttccctgta cactgtgaca aagaccagtt
    86401 attttaagat gtggatactt aagctgcatt gctctgcaaa gcatggccat tggattggta
    86461 tagtttagaa atactgcatt agggcagtat ctgtctatat ccttacagat atcgagcatt
    86521 acaggaattg ttcttaatgc tctaaatatg cctgatgggc ctctggtatc tccgacattt
    86581 atatcgacac catatttttt tggaatgagg atgtcatact gccatacatc aacatctcct
    86641 gctaaaatgg tacataaaac tccatcagca tctttaagcg cttcaatgcg gttggttgtt
    86701 gcggttacct ttgcaggata attaccagct ttaataattt tctttacggc ctgatgaatg
    86761 tagtcaagtc tttcagtatc aatatccatc agtacaatgt gggcatcttt gaaagcaggg
    86821 aaggtaagaa ggtcttttac aagattacgt gtaaaaccaa aactacctcc tcctataaat
    86881 gcaaattttt tcatggttca tctcctcgta gtgatatagt tacacagata ttgtaacact
    86941 ttacatgcag tatggaataa aaattacttt tcttttttat ataaaggtgt ataatgaatt
    87001 gaaaactttt gaaggagtaa tatgataagg gaagatttaa ggaatatagc cataatagca
    87061 catgtagatc atggaaaaac aactttggtt gatgcaatgt tgaaacagtc tggagtattt
    87121 agaaaaaatc aggttgttgc tgaaagagta atggactcta atgatcttga gagagaaaag
    87181 ggtattacga ttcttgcaaa aaatgcatct gtcagatata agaatacaaa gataaatatt
    87241 attgatacac ctggacatgc agacttttct ggtgaagtag aaagagtctt gaaaatggtt
    87301 gacggagttc tgcttttagt agatgcgttt gagggtccta tgcctcagac aagatttgta
    87361 ttatctaaag cgctggaact taatcataag gttgtaatag ttgtgaacaa agtggacaga
    87421 gaaggtgccg atatcgaaaa aacagaaaat gatgttcttg aactgttaat ggaacttcag
    87481 gcaaacgata tgcagctaga cagcccatat gttttctgtt cagcaaaaga aggagttgct
    87541 tcattatcag caacggaaaa aggggtcgac ctgattcctc tttttgatac gataataagc
    87601 catatagatc cgcctaaagg agaagagggc aaatcgcttc aggtactcat atcatctatt
    87661 gattacaatg attatgtagg cagaatagct attggaagag tagaaagagg aaccataaaa
    87721 gctaatagcg aagtagctgt agttaattac cacagaccga aagaacagta taaagccaaa
    87781 gttacaaata tgtatggatt tgaaggtgta aaaagagaac caatagatat ggcgaaagtt
    87841 ggagatataa tatcgttttc aggaataccg gacatatcca ttggtgatac tatatgtgat
    87901 attgagaata ttgaagcgat tgaattcgca aaaatatcaa tgccaactat cgaaatgact
    87961 ttttcagtta atgacagccc ttttgcagga agagaaggaa aattcgtaac atcaagacat
    88021 ctgagagaac gcttattcag agaaaccatg aaagatgttt cacttaaagt caaagaaaca
    88081 gaaacagcag aatctttcat cgttatggga agaggcgaga tgcatctttc aattcttgtt
    88141 gagactatga aaagagaagg atatgagttt caactcagca ccccaagagt gttatacacc
    88201 gaagtagatg gagtaacaca agagcctatg gaacatgtat atattgatgt ttcttcagac
    88261 agtgtaggtt cagtatgtga aaaaatgggc agaagaaaag gtgagatgtt atgcatgaat
    88321 ctcacaggca acagaatgaa gatggagttt attatacctg caagaggatt gttcggttat
    88381 aggaatgaat ttttaacaga tactgccgga gaaggcataa tgaatacatt atatgaagga
    88441 tatgctccgt ataagggaga gatagaatat cgcagttccg gttctttgat tgcttatgaa
    88501 acaggtacag ctgttaccta cggattatat aacgcacagg agagaggaat actttatata
    88561 acctctggag aagaagtata tacaggaatg gtagtcggag aaacgccaaa agatgaagat
    88621 ataactgtca atgtgtgtaa gaagaagcat gtaaccaata tgagagcatc aggaagtgat
    88681 gaagcgctta gattgtctcc accaaagaaa atgagtcttg aacaagccat ggaatacatt
    88741 aaagaagatg aacttcttga aataacccct ataaatatga ggataagaaa aaagatatta
    88801 gatcctgttt taagaatgag atcaaggata aagtaaaaac tgaagagtac tattgccttc
    88861 caatatgata atagtcatat tccttgccta gtatggaatg agatattttc aatgattcta
    88921 ttaaatcatt gtatatcttt tcattaatga tggaacttga accgtattca tactcattaa
    88981 ttgcatcatg aacaggtaat acataaaatc tggatccacc ccattccgga gtaagcatat
    89041 acagcgggac ataatcatat cctgtaacag tggttacatt agtagctaaa tccttttcga
    89101 tatacaaatt aagtattgcg gatgtcaagg tgtaatgagt tctagttata tttgacatga
    89161 agtttcctaa agagtagcag acaaagactt ctttttcttg attgtcagta gtttgtatag
    89221 tttcaaatcc cattggctgt aaaacatgtg tatgattgcc aattataata tcagctccat
    89281 tttcaaaaaa tagctgagct gcttctctct gactggcatt ttcagaagtt acatattcag
    89341 agccccagtg caaaagagcg attataagat cagtatcaag ggattttgca tattccatat
    89401 ctttttctat ttgtgtctta tttaaaacag agtaaggacc taagtaatct tcataaagaa
    89461 gattaacgct gaactctttg cctgctggta ttcgtatgac attagtccca tatgaatagt
    89521 taacaaaagc catactaatt ccatttactt ctttaacaag aacgccatta ttttcatcac
    89581 gctgttgttg tgttgcgtat gtgcctaaat gttccagtcc ggcattttca acattctcga
    89641 ttgtggaaac cagtccgtta aatcctttgt caaggcagtg gttatttgaa aggcttacaa
    89701 tatcaacacc aatatcctta agattcttgg ctaaagcagg aggagtatta aaaacaggat
    89761 aacctgtata ctctccttct gacatagatg tttcaagatt cgctattaaa agatcagtag
    89821 tttttgtgta gtttttaaca cgacttaacg aattcgaata atcgtactct ccggtttcag
    89881 cattatatgc atccagaatc tgggtatagt gactcattac atcaccgatt gctgaaagct
    89941 tcaaagttgt ttttgttggt ggttctggtg tttcaccaga aggttctgca gaaggagtag
    90001 ggttttgtga cggtggtttt tcagttgcag aatctgaagg tgtaactgta gatatttgtg
    90061 atggtgatga aacagatggt gtatttagcg gagtgcagga actaagtatc agtatgataa
    90121 ctattagtga tattagtata ttaatttttt tattcatata ttcccctcgt catttgtgtt
    90181 ttatggttct gtagaaggtt caggaacctt attagaagta tacccgttca aaacataact
    90241 gtaaagcctg catattatag cagcagcttc tgatctgtaa agttctgagt ttggtcttat
    90301 ggaattatca ggatatccgg acaggacttt catttcagag aaataggaaa ttgagtcata
    90361 tgcccaatat ggtattgtat ctttatcaat atagtcaata tctgaagatt ggcctatgcc
    90421 gaacgctctt cccataattg ctgcagcttc tgctctcgat ataggatctt ttggtcttaa
    90481 atatatttta ccttcactat caacagaacc tgtgagatat ccatatatgt atgctgcttt
    90541 tatgtatgga aggctccact gagcaatatc gtcataatct tcgaatactt ccacatttaa
    90601 atctaaatct tttacaggta tccccattgt ttcaataatt accttaataa actcctggcg
    90661 agtcatatgc acatatggta tgtatatata acctgttcca ttgtagactc cattaataat
    90721 tcctaatctt gaaactatat ggatgtatgg agcagcccag taatcctgtg atatgtcatc
    90781 ataattaaat acatatatct tgttagtagt tctttcctgt gcctgaacat ttcctagtat
    90841 tgatatatga tcttcatttg ttttaactat aatcatgtct cttccacatg aaatagtttt
    90901 aatattagtt aatgtgggta ttatctgaga taatggtgtt gccaagcctg tatatacaag
    90961 tgttccatca gaacgtaatc ctattgcata attatatcct gcaaaaattt caattatctc
    91021 ggtccagtct gatgtttgag tttgtctaaa ggaattattt ccgacagcta taacagttcc
    91081 atctttcttt aatccaagcg tgaaaccgtt tcccgcagca atagatacta tatctgtcca
    91141 gcctgatgta ttacattctc cgaaataatt cgttcctaca catctaacag tgccgtttga
    91201 cctaagtaat acagtatgca tggacccgca ttcaactttg gatacacctg ctatgatact
    91261 tgtgttgcac tgtcctgcac tgttgcttcc ggtagataat acagatcctg ttgatgtgac
    91321 ggccacggtg tgaaatgaac cagaagaaat ttgttttatt gaagaccaat ggttaacatt
    91381 gagagcataa gaatctgaaa agccggtata cagcaatttg ccatcataat taagccctaa
    91441 agaaaaggag tcaccagtag caacagttac aatatctttc cattcctgta cgtccgtttt
    91501 tccaaaagat gaatctccag cagtagaaac agtaccattc tcattaatcc aaagaccata
    91561 tgataaacca catgaaatag aatttttata atcaannnnn nnnnnnnnnn nnnnnnnnnn
    91621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    91681 nnnnnnnnnn nnnnntcatt aatccaaaga ccatatgata aaccacatga aatagaattt
    91741 ttataatcaa tatactcaaa agaaaaagga gtctctcttt cttctggttg tatctcagaa
    91801 tgaaaggaaa gagaatttaa gtcagtgttt aatgctgata acagattcaa tttcccatat
    91861 ccatagtaat tatttcgtcc tgcatctgta agggaatcag cactatagca tagtatagtt
    91921 tcaatttcga gaggtgtaag atctttgttt attgctttta ggatagctac tgcaccagtt
    91981 actattgcac aggaaaagga agaaccgctt gcaaaggtat atgtaccatt aatacctaaa
    92041 gtaaagatat cctcaccctg tgcacatatg tctattctgt cattgtatga tgaaaaagga
    92101 gaggaaagaa aatattcgtt gttgtataaa gagagagcag atactgaaat cacaccataa
    92161 tttgatgaag gatatgaata gtattcattt cctttatttc ctgctgagga aacgataggt
    92221 atattttttt cataagcagc caaacaggca atctgtaaat cttcatcata atgtgggata
    92281 tctatactta tatttataac atcaacattc atgcttatgg cataatatat tgcgtcaata
    92341 atatgctgta agcgtaaagc accagttata tcgctataaa cttttagcgg taaaatttta
    92401 atgtcaagat tgccgcacac tccataatag gcagtgtctt cagtaggtac tcctgcaatt
    92461 attcctgcaa tagcagtgcc atgtccttct ttatcctcga aatcagtgat agcggatgaa
    92521 gcaacaaaat tacgtccatt ttccaaatca atacggtctt ttaaatgctc gttatctgtt
    92581 tcaataccag tatcaataac agcaactgtt acaggcttgg tctccatatc agataatact
    92641 ttccatcctg caggaatagc agatgagtac agccagtaaa aacgggtatc aaaactttca
    92701 tcaagggcat ttgctggtaa agtttgaata aatataacta ataggagaag ccacataata
    92761 acgcgcttaa aatttttgtt catactaata taatataaca atattcataa taagtcagta
    92821 ctttttgtga tgctattgaa ggatgtgact ttttccggtc aaagtatcca gaatatagta
    92881 attatcatta tcttgattaa gatagataat atatgaatca agtatattta tattcgcaca
    92941 gtcatcactg gatatggtaa tctggtttag agtattaatg ttaactttct taagtgaacc
    93001 attagcggct ttgaaatata tgttttcagt atcattagga ttacttgata aaaaccagcc
    93061 tttgacattt tccagtcttt tattatcttt accatcaaaa gtcatagaat aaatataaga
    93121 ctcatcatta atatttgtat aatatagtcg gtcnnnnnnn nnnnnnnnnn nnnnnnnnnn
    93181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    93241 nnnnnnnnnn nnnaagtcat agaataaata taagactcat cattaatatt tgtataatat
    93301 agtcggtctt gaagaacagt aaagaagatt gtgtgctggt ttaatataga aatggattca
    93361 ttgttttgaa gatcaagtct gtagatactg ttattatcat aatagttagt aaagaagata
    93421 taatcatctg ttacaaacat agaagcagca cttccttctg ataactttgt aactgaaaga
    93481 tcgtacatac tcattttata tattgagttg ttttcctcgt tgtttatgaa ataaatccag
    93541 tcacgggtga cataaagatg agagcatatg ctttcataca gtgtctgttc ctcggaccct
    93601 gaagaactga tttttttaat agtaccatta ttgccatcaa taaagtaaac atatccttga
    93661 aatacattta agtattttgc attagcagaa aatattgatt tttcatttcc tttttttaaa
    93721 tcttgtttaa agattttaaa accagcgtca aatttagtgt aataaagatt accatcttca
    93781 tatgaagcga tgcccatgtt tattatgtta gaaggggtgt tgccttctat gtcattatat
    93841 gatttattga aaagagaaaa tagaacaatg gaagcagtaa taagtataac ggctaaacct
    93901 gccgcaataa tcacagtcct atgctgtttg tttctcaaat tgatcttaaa cttctttttt
    93961 atcatactca tataactcca acgttcaata ttatactatc attttactaa ttggtatata
    94021 agtttttgtt cattaggagc actttttgat attttgactg cttttttata ttcattttca
    94081 gcttcactga gtattccttt tctgtttgta tatagagttg caagaacctg tcctctcttt
    94141 atcttatcgc ctgttttagc atgtatatat attccagcag aataatcgat actatcttct
    94201 tttttctctc ttccagctcc taacatcaaa gatacctcgc caatggcagt agtattcata
    94261 gaataaataa acccatcttc caaagccaaa acatcatatg aaaaagctgt tttttcaaat
    94321 aaatccagat tgtctatgta acggacatct ccaccttgct gaataacaag atctctgaac
    94381 ttattaaaag ctgttccatt ttttaaagat tctacaactc tgcttttgca tttttcatat
    94441 gaatcgtttg tgcaaagcca taccatatat gttgaaagtt caacacatac ttcagttaaa
    94501 tcacaaggac cctgattttt aagcacttca caagcttctt ttatttccat gctgtttcca
    94561 atagcttttc ctaacggaac atccatatta gtaattaaag cagcaatgtt ccttcctgca
    94621 agttttccta cttcaaccat tttttctgcc agtttcactg catcttttaa atccggagta
    94681 aaagaaccag tacctacttt tacatccagt aaaatatttt gcgaacctgc agctattttt
    94741 ttactcatta tacttgatac tataagagga agagaaccaa cggtagctgt cacatcacgt
    94801 aatgcatata ttttcttgtc agcaggagca agatttccac tttgacctac taatgcaatt
    94861 ccaattttat taacattgtc aaaaaattcc tgtttatcta aggaagtcct gtaaccaggt
    94921 attgattcta gtttatcaac agtcccacca gtatggccta aacctcttcc gctcattttt
    94981 gcaaccttgt atcctaatgc tgcaacgcaa ggagcaatga ttaaggaagt tttatctcct
    95041 acgcctccgg tactgtgttt gtctactgtt attccgttta tactggaaag atctattaat
    95101 tctcctgatt cagccattgc taaagtcaag gttgttgttt cgtgatcatt catgccatgg
    95161 aaataaatag ccattagtag tgctgcagcc tgataatcag gaacagatcc ttctgtatag
    95221 ccttttacaa aatatcttat ctgctcgtca gataaaacta gaccgtctcg tttttgctgt
    95281 atgatatcat acattctcat aattaccgtg cccccttgtc ataaggctgg cccactgctt
    95341 ttggagcttt gctggctttg gatgaaaaag ctaaaacaat cagtgtggcg acatatggtg
    95401 tcatcttaaa aaactctgaa ggtatattca gatttaacaa gaaaggaata gctgagtaag
    95461 cagcagatat agtctgcata aatccaaaga aaagtgctgc ccacaatatc cgcataggtt
    95521 tccattgtcc gaagataaga actgcaagag ctaagaaacc atacccgtta actgcaccct
    95581 taaattctgt agagatgggt attgtaagca ccaaaccgcc aagacctgct aatgcgcctg
    95641 atatcattac ccctatatat ctcatcttgt aaacattgat acctaccgaa tcagctgcct
    95701 gaggatgttc tccgcatgct cttaaacgca ttccgaaacg cgttttgtat agcaccactg
    95761 ttgcgattat aagaataagg aagcctataa aggtagttat atatgtattt ttaaagaata
    95821 catcacctat gaaaggaatt ttacttagta aaggaacctc agatatctga aaagtatttt
    95881 tgaaggatac gtttgctata cctgaaggtt gtatcattct tgcaaggtaa attacaaaag
    95941 caggtgcaaa catattaagt gctgttccgg atatgacctg gtcggctttc agatttactg
    96001 aagcatatgc gtgtaaaaga gaaaacacta cccctcctgc aatagccatt attacagcta
    96061 aaagaagcaa aagctgaccg ctcattccag actgcatctg gttaatgaag taaatgcctg
    96121 tgaaacctcc cataaccatt attccttcaa gcgcgatatt tgtaacaccg cttctttcgg
    96181 aaaacatgcc tccaagagcg acgattaata atggaatgga aaagaacata gtctgtctta
    96241 gcaaaagata aagcatatcc attaactttt acctcctttg ttgtttctta gtgaatcaat
    96301 aacagtttca gttatgccag attgattcct cttttcgtcc tttttcagtt ttttcatttc
    96361 taaataattc cttataatca gagatgctgc agaactgtat atgatgattg ctgttataat
    96421 ctgaatgaat tcaggaacaa aactgtatct ttgcatatag aatccgccta tttgaagcca
    96481 tgcaataaat attccagaga ataatattcc tatggggctg ctcatgccta aaagagcaac
    96541 agatattcct gtaaatccct gcgcagctaa aacgtcaact acttcaatgt atactccagc
    96601 tcctgaaaga tagaataatg caccacctat accggcaagc attcctgcta ttgccataga
    96661 caatactatg cttcgagtct cattaattcc agcatatttt gcagcatatc tgttatatcc
    96721 gcatgccttt aattcataac caaaggttgt tttttcaagt actatataaa tcagtactac
    96781 aaacagtact gcaactaaga atcctccatt aatacttgag ccagggaaaa tggtagttaa
    96841 ccccatttta ggaatgtttg cagttgctgc gacaggtaaa gacctgtttg tgtaaacatt
    96901 taaaacagtt tgtttaatta agtaatttac taaaaacata cctatgtagt tcatcattat
    96961 tgaagatatg acttcatgaa catttaagaa tgcttttaaa atacctggaa ttgcacccca
    97021 tatggctcct gcaatcatag cagctaatat accgacgacc cattggaaac cgccaagtac
    97081 cggaagtttt actccaacta aaactgcaac atatgcaccg actatgaact gtccgctggc
    97141 gccaatgtta aataatcctg ttttgaatgc gaatcctaca gcaagacctg tcattataat
    97201 cggagtagca aaatagaaga catttcccaa acttcttgta ccatcgttaa agcctccaac
    97261 aagcatagtg aaaaagcctg caaatgattc agcgggattg gacacaagaa ggattacaat
    97321 accgataacc aaaccaatgc caattgctat cagggaagaa gtaaaattcg ctgtcccttt
    97381 ctttcttcta aaattatcaa aaaattcact taatgaagta ttgtttttca tgccttcacc
    97441 tcatctcttt tggaaccagc catatagaga ccaagttcct gaacagttat ctttttagga
    97501 tcaagttctc cgactatctc accctcataa ataactaaaa tcctgtcact taagttcatg
    97561 acttcctcaa gttcaagcga gataagcaga acggcctttc cggaatctct ttgttctacc
    97621 agctgcttat gtatgaattc tgttgcgcct acatccaatc ctcttgcggg ctgtactgca
    97681 atcatgattg aaggttcctt atcaatttcg cgtgcaagta tgactttctg ctggtttcct
    97741 ccagacatgg atcttgtcgt tgttaatgga ccttgtccgc ttcttatatc gaatttctct
    97801 atcaaagtct ttgcatattc atttatactg tttcttttta agaaacctct tttttgaaat
    97861 ctgtcagtaa aatatgtctg taataccaag ttatatgcca tactgtattc cagtacaaga
    97921 ccgtatttgt gccggtcttc aggaatatgg cttaaaccat ttgagtttct gtacctaatg
    97981 ctcttttttg tccagtcttc accattaacc agtatctgac cttcatcaaa atgctgtatg
    98041 cctgtaaggg catatacaag ctcactctgg ccgtttccgt ctatgcctgc tatgcatact
    98101 atttcacctt ggcggacctt aaaggatatt ttattaagag tgtttcttgt acttcctttt
    98161 gttttcatgg aaagattttt aacttcaaag acagtttctt taggtttcaa tgcttttttg
    98221 tcaatagtaa actgaacctt tcttcctacc atcttttcac ttaagaattc ttttgtcaca
    98281 tcttttacat tatatgtacc tatacattta cctcttcgca aaacggtaac tctgtcggca
    98341 actttcttta tttcttcaag cttatgagtt atgaatatca aagacttacc ttccttggaa
    98401 agttctttca taatacccat caactcgtct atttcctggg gagttaaaac agctgtcggt
    98461 tcatcaaata tcaatatttc attatctctg tataacattt tgagtatttc tactctttgt
    98521 tgcataccta cagttatatc agaaattttt gcatcgggat ctatataaag gttatatctg
    98581 ttgctgagtt caattacctt ctttttggct tcatccattt ttaagaagcc attcttaact
    98641 gtttctgctc caagaataat gttctgcaat actgtgaaat cctcaacaag tttaaaatgc
    98701 tggtgaacca tacctattcc cagattgctg gctgtccttg ggtcagtaat attagcttct
    98761 ttacccctta acttaatgat acctgcttca ggacgatata agccaaaaag aacgctcata
    98821 agcgtacttt tgcctgctcc attttctcct aacagagcat gaatttcggt tttcttaatc
    98881 tgaagagtga tatcatcgtt agcgacaatt ccgggaaatt ctttcctgat gttcagcatc
    98941 tcgatgatgt aatccatgaa tttactcctt agtcggttaa ttattgtacg tcgcttatta
    99001 ttatatatta attttaagta ataataaata agtagttata actagtatag ataagcaatc
    99061 ataaaatata tgaaactgaa aaatgtgtat aaaaaaactg ccacca
//
LOCUS       DAOSXM010000003        58751 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2111
            NODE_324_length_58751_cov_10.552065, whole genome shotgun sequence.
ACCESSION   DAOSXM010000003 DAOSXM010000000
VERSION     DAOSXM010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864178
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 58751)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 58751)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:24:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,261
            CDSs (total)                      :: 2,229
            Genes (coding)                    :: 2,218
            CDSs (with protein)               :: 2,218
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 3 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58751
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_324_length_58751_cov_10.552065"
                     /isolate="AMR_MDS_2111"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(136..1698)
                     /gene="rho"
                     /locus_tag="PLQ68_00985"
     CDS             complement(136..1698)
                     /gene="rho"
                     /locus_tag="PLQ68_00985"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015360013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HXK71253.1"
                     /translation="MNEDEILRSKSIADLRVIAKTLGIKSVTIMKKDDIIKRILEITK
                     PEATNKEEPEEKQEAPVVEVTKILETVTQDAPKKPRYLLSKMAKESESGKPVKEEKRT
                     RHVIKAHDESEIALPEKKGKEADQVKEFSQVSEPDMAEQFDSEQYDEVSGVLEVLPDG
                     YGFLRTDNYSYGSKDIYVAPNQIRKYGVRTGDKIIGTGKPKKETEKFQALQTVLKIND
                     DDPSQVFKRKPFETLTPIFPEERLTLENSPKDYSTRIIDLIAPIGKGQRGMIVSPPKA
                     GKTVLLKKIANSITINHPNIELIVLLIDERPEEVTDMQRSVNGEIVFSTFDKVPENHV
                     KVAEMVLERAQRLVEHGKDVVILLDSITRLARAHNLTMTPTGRTLSGGLDPGALHAPK
                     KFFGSARNIEFGGSLTIIATALVETGSRMDDVIYEEFKGTGNMELHLDRKLSEKRIFP
                     AIDINKSGTRREELLLNKKELESIWTIRRAMSNMGTAEVTEWMIDKLVKTKNNSEFVN
                     SINIKQSNFTEY"
     gene            complement(1803..2579)
                     /locus_tag="PLQ68_00990"
     CDS             complement(1803..2579)
                     /locus_tag="PLQ68_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014354466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HXK71254.1"
                     /translation="MNEVIKNKEGFIIDMDGVLYHGNNVLEGALEFVDWLKANNKKYL
                     FLTNSSERSPKELSQKLARLGIEVAEEHFYTSAIATAIFLSTQMPKCSAYIIGEPGLI
                     NALYDVGITMNDMSPEYVIIGETRAYSFEKIEKAVQLIQKGAKLIGTNPDKTGPTEKG
                     IMPATGALAVPIEIATGKNAYYIGKPNPLMMRHALKKLGTDVKSTLIIGDRMDTDIVA
                     GIESEIDTVLVLSGVTNEDNILNFSYRPKYIYNNVGEIIV"
     gene            complement(2572..3876)
                     /locus_tag="PLQ68_00995"
     CDS             complement(2572..3876)
                     /locus_tag="PLQ68_00995"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HXK71255.1"
                     /translation="MKYQLVLAGGGARGSYQIGVWKALRELNIEIDMVIGTSVGSING
                     AAVVCDAFDQAEHLWQTLDMPTLFNITYNKEDGEPNTSKYLESILKNKGLDTTTLREY
                     LNKVIDEDVLRASEIDYALVTFSLTQMKPVILFKKDIPQGKVVDFILASSAVPGFKKQ
                     EIDGDLYIDGGIYDNMPINVLIDNGYKNIIAVSNSAIGMYRKVKESDAKIIIIENERT
                     EGGILEFDPEIAKRNMLLGYLDGLKAFGKYIGKCYYISDNAKSKYAMALTEKEIEMLY
                     LNIKKNKNNIMNKVVQFSILRTLRNHSDGTLDAKKAFFAAMEITADIFGIEKIRAYTP
                     EQLITKILIAYRSVQTEKKEFEKSKKNIFKFIWGKIWDKNKVRINKKAVQKLFLGQVL
                     IDRDNLSINSNTTAEYLISNLFIYLILWRRKRIRKQIGVENE"
     gene            complement(3939..4706)
                     /locus_tag="PLQ68_01000"
     CDS             complement(3939..4706)
                     /locus_tag="PLQ68_01000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK71256.1"
                     /translation="MHTKGSITAECCICSVVIVFALLLSIQLIGYAKANFTILQDLEC
                     ELRNAAVTYHISDIYLLNPLKKDRYECVKNIRTMALPYEYFFTMSARSIYTGVLANNN
                     MHIKAISPKWKGDGIGTAECNIWDLSPFERGDVIHQIMGANLEKDFPVLDMYDEYSRE
                     AVSIISINTQDSSYLSGTDMKKKIKQQIDAIDSYTIQSLRNVTIDETDIEKKKVLVVI
                     PNEALNNRQRKQIDEMTRYADSLDIELEIKKFQGASN"
     gene            complement(4696..6690)
                     /locus_tag="PLQ68_01005"
     CDS             complement(4696..6690)
                     /locus_tag="PLQ68_01005"
                     /inference="COORDINATES: protein motif:HMM:NF039199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5702 domain-containing protein"
                     /protein_id="HXK71257.1"
                     /translation="MVFLKHYGNNKKGIISVFVAIVLPVILILSLVLSDIYIAKNASD
                     AAKNALKASCGCVLSQYSSFMQNEYGLYGYTIDDGKAYDIVFKALDKSSISTGIYKMD
                     IQNIEVTKTNPIYDYSVLSSQVFTLMKDDIYKDILIEVNEKIDIFKKMKDVLKTLNIK
                     MQVDELVGGLKNVHEKLKTTIEQINSSSYYQDLIYMIESVDYIVHNIHDLIDNAENID
                     NVNAIELLKEEALEIININIDNLVYVLKEYNKQGLNLISQMVHTYAQVHILSDSMRRI
                     VSSIKDCPEYLEVFLEICIDTVYSMENSFSNTVFDQIERTFDRNLKCLENALKDITEA
                     VISGSEQPILYVFNDAMEEYYDVVYNPELLDSFFFTLFEDNNEDKRGFWKEYAKNMLN
                     SLDFDDNTIDSFIALISDDEFLDFTIEADMTGNTSKSIFLSIDDICSMLSKLEEDIYF
                     NEYLMLYLNSITDSEKNVSKSKYLKGETEYIIFGNRDNSKNIKSCEAAITALRFALNI
                     MHVYTDIDKTTKANVLAASLAGSLSFGAATPIAKNLILCSWAFAEAAYDTNTLKRGEK
                     CPLFKLDGDWNIDIGIDSGFNKTPDWLKMDYEDYIRVLMCTTSNDQKTKKLLDLISIN
                     CPYVIDMSQVYCGINVKAVLSYEGIFGIKRIYKFEVSDAY"
     gene            complement(6662..6883)
                     /locus_tag="PLQ68_01010"
     CDS             complement(6662..6883)
                     /locus_tag="PLQ68_01010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK71258.1"
                     /translation="MDASLSTSKKGYACTEAAIALPFIILLSIVVIFSITSISSKYEQ
                     IEEYNLTEDISKADSLKRKADGFFEALWE"
     gene            7011..7739
                     /locus_tag="PLQ68_01015"
     CDS             7011..7739
                     /locus_tag="PLQ68_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002673401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5058 family protein"
                     /protein_id="HXK71259.1"
                     /translation="MFDPNSNLMYIMVAIIILYVAAQAVYFLIKAVKRAKELNISTST
                     VKKTILSSAVFTIAPAISILMGVLTLAGFMGLPFPWLRLSVLGAITYELSAAYSAAES
                     LGIELTSLVTDAKVFSTMAWVMHLGIFTGLILVPLFLKKIQKGLVLIKRKDEKWSDAL
                     LTALFLGMISAFLGLVFSDIKSGLEGWIPVFVMIVSALIMSVIGILVKKLKIKWLEDF
                     ALPITMLSSMALAIPITNLVQSIL"
     gene            7749..8420
                     /locus_tag="PLQ68_01020"
     CDS             7749..8420
                     /locus_tag="PLQ68_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009002797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK71260.1"
                     /translation="MKKKTIRSYDDQIHFSGRLWMLGALVLFTMLPLTISVLYDAWPS
                     AGPFFKALAGVAPIFWTVGLIEIITYTPMLGSAGSYLSFVTGNISNLKAPVALNAMNS
                     ENVKPGTDEGEVISAISIAVSSLVTIVIIAIGVLGLSAIRPLLESETLKPAFANILPA
                     LFGGLGVVYVSKNWKIALAPLIFMLILFIVSPIDLSGYVGILVPVGAIIAIVSARILY
                     KKNKI"
     gene            8430..9863
                     /locus_tag="PLQ68_01025"
     CDS             8430..9863
                     /locus_tag="PLQ68_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009267353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HXK71261.1"
                     /translation="MWWLVFPAVLLAFVIFIVIRAFLFKPYDSQAPKTQEFEIDEENI
                     VNRFSKIISCKTISNRDLSKVDIGEFEKVQDLIKKFYPNIFKICEFEKVLQTGLLFKW
                     PGEKSDEPVVLMAHYDVVPANEQQWEKPAFDGIIENNELWGRGTLDTKCTFVAILEAS
                     EQLIKTGFKPKNDIYLSFAGDEEVAGPTCPAIVELLKQRNIHPAMVLDEGGAVVENVF
                     PGVSGKCALVGIAEKGITDIRMSMKSSGGHASAPPPHTIIGQLSQAVVDIENHPFSFQ
                     LTPPVSQMFNILGRHSSFVFKIIFANLWCFKPILNLMCKKTGGELNALMRTTVAFTMA
                     QGSQAANVLPPSAEFTANLRLLGKDTYESAVLHMKKVTDNPDILFEVIHGSNGTTYSS
                     TDCIAWDTIKNAISQTWPDALVSPYLMFAASDSRHFCKITDKVYKFSGMHLTKEQRGY
                     IHGNNERISLDAIYDLVRFYLRLISLV"
     gene            10024..11406
                     /gene="cysS"
                     /locus_tag="PLQ68_01030"
     CDS             10024..11406
                     /gene="cysS"
                     /locus_tag="PLQ68_01030"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HXK71262.1"
                     /translation="MKVYNTLTRTKEELVPLDGKTIRMYSCGPTVYSYAHIGNMKAYI
                     FMDFLKRSLNYLGYKVKSVMNVTDVGHLVSDGDEGEDKMMKTAKTANKTPWEIASIYS
                     DAFFKDISKLNIQKPNVVCNASEHINEMLDMVYKLYDDGYAYETSDGIYFDIMKFPDY
                     GKLSRMDLEGQMAGARVAVNEEKKHPADFAIWKKAPKEHIMQWNSRWGMGYPGWHIEC
                     SAMSRKYLGDQFDIHTGGVDHIPIHHENEIAQSDCYIKKQAVKYWMHCEFLLVDNGKM
                     SKSIGTAYLVSDLEDKGYEPLAFRYMCLNAHYRSKLNFTWDGIEAAQKSLVRLSDLTL
                     AHKQSKEDVPDELISNLKKEFTDALSDDLNIPLAMGTVWNAARNSTKSHKIYELLMDF
                     DKVIALNLDQKREAEPEQLPDDIQELVKIREQARKMKDWKKSDELRDQLNSAGYLVND
                     TTSGQIIKKA"
     gene            11408..12268
                     /locus_tag="PLQ68_01035"
     CDS             11408..12268
                     /locus_tag="PLQ68_01035"
                     /inference="COORDINATES: protein motif:HMM:TIGR02494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HXK71263.1"
                     /translation="MLVNIFQRGFNFSQDGPGNRLVYHLQGCNMHCPWCSNPEGISEK
                     GSIIISQGISDKVCPMNAITDGLFDSKICKGCQRMCLDHSHLGIKLSCETFNTDEIID
                     EVIRSKSMFFNNGGVTFTGGEPTLNLPVLTDLLKKLKEININTAIETNGSHPELASIL
                     PYVDHMIIDFKHYDDDKHRKTVGISNSIIKRNLKKLSFLAKINVRIPLVNGFNAQEEN
                     AHGFAEALAECNKANITVEFLKFHEYGKAKWKQCGMEYKMTGNEYVSQETVDMFIRIF
                     NEKDFTTITT"
     gene            12322..14685
                     /locus_tag="PLQ68_01040"
     CDS             12322..14685
                     /locus_tag="PLQ68_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004607495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase family protein"
                     /protein_id="HXK71264.1"
                     /translation="MKTYDIYTSEKINKKAALLFKEERSLNRLDGWFIAREIVCNHNS
                     ELNGLPKEIRAANELKYIISEIPLSISEHNIFAGTQRDAFARSYALINPAFEVETFSG
                     YCDPTAIYDDIEPNEQFTAERIERVKKFTKNTEFVKQLTKVYDEYEQYTKEVVFFIEQ
                     VTGHVIPDFRPALKYGIKAIIEETEKRISSEADEYKKNNLKAMILTLESAVLLANRYA
                     DIAVLQMKTASEERKKELNLLESTLRKVPYMPAKNLYEAIQSYLLLWQVMCIEQAPNP
                     FAFSVGNADRIFEPYRNDLSREQAASLFKHFLVFFNVGDRSWAISQNVLLGGESNNGK
                     DQTNLCTYAFLDAYYAMNFPQPILSVKLHNNTPRQLYEELGRFFFTPGCLTPSLFNDE
                     SVFKVLSKAGVDENDLEDYSVAGCQEPLIMGKDNGNTTNSWLNLGKILEMTLNNGKSL
                     ISGEQIGRKRTVTASDLLKNIKEYFYDDAKYYIKLMTDAANRASIALSEMPVPFLSSF
                     MGGLESGYDMRDCKYQGTKYNASGCLIHGLSVIADSFVAIDEYLKKYPDDPDRLISAI
                     RSNFENDQDLRAFLKSTPKYGNNLTVPDNLAMEISKEISDYVKSMPNYLGNNFRADWS
                     TPSTHLLYGYWVGATPDGRLSREMLGYGIDPLYGEATTGLGFRILSCMNLPFDEMNGG
                     YASHFGIDPKFFTAKSDEEKGLQFKERIIQPLFFNEQNQKLCPYYLYVNVTTAETLRK
                     VLEDPKTYAPSGVYIMRIHGTFVNFLDLSPAIQNDIITRLDPDSTSI"
     gene            14695..16062
                     /locus_tag="PLQ68_01045"
     CDS             14695..16062
                     /locus_tag="PLQ68_01045"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HXK71265.1"
                     /translation="MLALGLDIGTSKICVTIIDCKSGLCIENFHDDNDTFIKINGKSS
                     KMQDAVAIKQKALTLLNTAIDKYPDIKCIGVTGQMHGIIYLDSDNNIVSPLYTWQDES
                     ADSNFHDLNISYAKKLSDITGYKMASGYGLATHFYHSDNKMIPKTEVKISTIHGYIAC
                     SLAENENIIIHSSDAHSLGIFNIKNSRFDKDAIEKAQINYSILPKTINKPYILGKYLD
                     KIPVCVAIGDNQAGFIGSGCTNDILVNIGTGSQISLSTNKSETKSSSVEIRPLNYDQH
                     IFVGASLCGGRAFAILKDFYTDVIKMANLPVPKNLYDLMLNNLENTDLTDSIVFNTLF
                     CGTRDDSSLNAGISNISTDNFNAGNMTNGTLEGIAVELYNMYKIIKKDDDNPKNRLIG
                     SGNAIRKTPPLRRILEKKFNMPLYMPKNSEEAAFGAALYACVAIGIYNNLEDAQQIIQ
                     FEEIV"
     gene            16059..17126
                     /locus_tag="PLQ68_01050"
     CDS             16059..17126
                     /locus_tag="PLQ68_01050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="class I mannose-6-phosphate isomerase"
                     /protein_id="HXK71266.1"
                     /translation="MKNNLNMLLNRPIFFERNRVFRVYKGGLLFNNLFNDKPEDGNYP
                     EEWIASTVKALNKISSGPKEGISKIEDSYIYLDDLIAEHEQQILGSQKDIGILVKALD
                     SAVRLPVQVHPDKDFSKKYFNSEYGKAEAWIVLDTRPGSCIYFGFEDNVTKQDLYTAV
                     KNSESDKSAMEKLIIKHEVKKGDVYFIPAKAVHAIGYGCFILEIQEPTDFTIQPEAWC
                     DDYKLNDYEMYMGLDMDTALSCFDMNISGKEAINISKVKPVNISEKKNVRIESLIDRR
                     NTDCFRVNRININRDSFLLKHAPSIYVVIEGNGIISCEHSSRLVKKGDYFLLPYCLKS
                     KCILSSNEKITVIESFKGETD"
     gene            17123..18181
                     /locus_tag="PLQ68_01055"
     CDS             17123..18181
                     /locus_tag="PLQ68_01055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK71267.1"
                     /translation="MIDFSDYPLSIISSISYGGHVQGIAVDKKNSFIYLSFTTELIKA
                     DLSGNIIGSIKGWIGHLGCIDYNDDDECVYGSLEIKNDSIGKSIKELLKTDKTIPDCF
                     YIAVFDTKRIDRKDINAKDSDILKTCFIKECTDDYINKRYGCSGIDGTCIADDIEDNH
                     DRLNYLYVAYGIYKNNQEGLNDYQIILKYTIKDILSNCISFNENPFHSTGPSKPDNKY
                     FIYTGNTNFGVQNLEYDGYTKDWLMAVYKGEKPNFPNYSYYIIDGKTKPEIKKIQQYS
                     DELYYNLLSLKKLPYSDSLTPGFNFERGQEGIYSFDNGYFYIAKSKRSEDLGWYAQID
                     MYKISYDSKNIFEKVVYG"
     gene            18174..19115
                     /locus_tag="PLQ68_01060"
     CDS             18174..19115
                     /locus_tag="PLQ68_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009756984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HXK71268.1"
                     /translation="MDNNSKHTSKSIKSSLMLLLTSFIWGFAFVSQRMGLATMGMHTF
                     NATRFLLAAFVLLLFILFTDIKDKKKAIVIGYTKNNKKQRRNLITAGTICGVILAIAN
                     TLQMAGLQFTTIGKAGFITVLYIIIVPLTGIFFKKHINLSVWVSVMLALGGSYLLFIQ
                     EGFSINKGDLLILGCAFLFAAHIMVIDHYTKITDSVRMAFIQFFVCGVISMIAMFIFE
                     QPDIKVILSAWLPIAYAGVLSSGVAYTFQFIAQKNLSAPVASLIMSMESVFAVLAGWI
                     IFQESLSVKEILGCVLIFFGILIVQIPFKNKKISKQI"
     gene            19175..19531
                     /locus_tag="PLQ68_01065"
     CDS             19175..19531
                     /locus_tag="PLQ68_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014794676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HXK71269.1"
                     /translation="MNIQESAEMYLETILILSKQADQIRSIDIVNYTGYTKPSISRAV
                     NLLKKNGYLEMDQKGFITLTKNGEEIAKTIYERHCVLTDLLIKIGVDEKTARHDACKI
                     EHVISNDTFEKLKEYR"
     gene            19584..20513
                     /locus_tag="PLQ68_01070"
     CDS             19584..20513
                     /locus_tag="PLQ68_01070"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HXK71270.1"
                     /translation="MIPRDRAVTALSGGIPDEIPTFELEFQLSMELLGKEFLKQEELN
                     KMSSKEKDIALHENAEFMIEVYEKLEYSIIPIHYLWIDDAIKTAKIINSMTNFKYLLT
                     RHGDGTYSIPDGNEMYEFAYRTIDDPKGLLEQAEAMAVNAIERNKKAMAGGIESFILC
                     CDYCYNNGPFLSPEQFRIFITPFLAKIIKGIRDEGGYAIKHTDGNIMPILDQLIEANP
                     HAIHSLDPMAGVDIAKVKAIAGNKVCLIGNVNCALLQTGTDLEVIDSAEYCLKYGKPG
                     GGYIYSTSNVPFKGLNLDRYLLVLDVWKKHRKY"
     gene            complement(20520..20693)
                     /gene="ssrS"
                     /locus_tag="PLQ68_01075"
     ncRNA           complement(20520..20693)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="PLQ68_01075"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(20785..21660)
                     /locus_tag="PLQ68_01080"
     CDS             complement(20785..21660)
                     /locus_tag="PLQ68_01080"
                     /inference="COORDINATES: protein motif:HMM:NF012933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA glycosylase"
                     /protein_id="HXK71271.1"
                     /translation="MKIKESFFTDYTILENANEDYLSLSDTFKCGQCFRWEQLNDEKL
                     FIGIAYEKILKAEQHDGFIKLNCNKEDYNNIWREYFDIDTDYDLIKEELRKDNIMYKA
                     ITCSPGIRILKQELFETTISFITSSNSNIKRISNNIRDLSIILGRKLGEERYAFPTVK
                     KLSSSDLCTINECRAGFRCNYILESSRMINKIVTDFNVNRYKSIGYEQTKKELMNFKG
                     VGSKVADCIILFSGISSYAFPTDVWVKRIMENLYIKKEVSLDYVQKYGQDKFGPLAGY
                     AQQYLFYYARSNNIK"
     gene            complement(21650..23656)
                     /locus_tag="PLQ68_01085"
     CDS             complement(21650..23656)
                     /locus_tag="PLQ68_01085"
                     /inference="COORDINATES: protein motif:HMM:NF013300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HXK71272.1"
                     /translation="MEDRLRFRQIHLDFHTSELIKDIALGFEPEKFAKTLKDAHVNSI
                     TCFARCHHGMIYYDSKVNKERIHPYLCDKDLLKKQIDACHKYDIKVPVYTTVQWDMYT
                     AIRHPEWLVRKPDGSIEGEYFNKDGFYQRICVNTPYKDFLKKHVREFVSELDCDGVFF
                     DIVSEMDCSCTYCMNSMLEKGLDPLVEDDRITHYKSVMREFKKEMSSLVWSLKPSILV
                     FFNSGHVSYKDKEPSKDTYSHYEIESLPSGGWGYMHFPVTCRYTRNLGKDYLAHTGKF
                     HTSWGDFHSFKNQEALEYEIFRMLALNSKCLIGDQLEPSGILSEPVYDLIGKVYSKVE
                     DKEPWCKNAQHIAELAVLVPTDEKSRVSASQAAIESILDQLSYQFDFIDKQMDFYKYR
                     LIIIPERIGIDSELEIKLNEYMENGGKVIAVYDSIFKGLSVKVINETVDDYKQPVKGR
                     FITRNGYSDYIMPNDKIGKDLYKTEYVMYAKGFETIADKGYSLVKAYESVFNRSYKHF
                     SSHLQSPSSHKEAYDAVILNRKKKAIYFSHPIFEIYNKKGPKWIKSILSDALEILLKD
                     KMVSHNGPSTLFMAMNEQPQENRYILHMLHYIPERRSTDFDTIEDVIPLYKTKITLKL
                     DKQIKNISLVPQSTPVKFNQKNNKISFIVDKIEGHCMLSIEYEN"
     gene            complement(23666..24475)
                     /gene="murI"
                     /locus_tag="PLQ68_01090"
     CDS             complement(23666..24475)
                     /gene="murI"
                     /locus_tag="PLQ68_01090"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HXK71273.1"
                     /translation="MINGKIGVFDSGIGGLTVLKEIIKQLPNEDIIYFGDGKRTPYGG
                     KSKQTIELFALQSMKFLTQRGAKAIVIACNTVSSNAMDAIKQHYDLPIIEVISTTAEL
                     AYNKSENKNIGVIGTKATIESHSYEKALKSMDTKVTVHQKSCPLFVPLAEEGVEWWED
                     EITKMVAKRYFSFFDNTDIDTLILGCTHYPLLKNSIRQTIGNDVCLVDSAYITAKQTK
                     IMLEKSGLLKKSSSKGILEIYTSDSIERFMPMCNAILGMDCLNINKCDVGE"
     gene            complement(24468..25547)
                     /locus_tag="PLQ68_01095"
     CDS             complement(24468..25547)
                     /locus_tag="PLQ68_01095"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HXK71274.1"
                     /translation="MKNICIIFGGISCEHEVSRISAVNVLRNLDKKKYHPYVIEITKD
                     RQYYLYEYHDNVEYYQQLVQINNKLDKEKIVPDIAMFQKMNIDVVFPVLHGTKGEDGT
                     LQGYLDLCNVAYVGSDVLSSAICMDKVLCKDILVKNGIAVADYLWFSCEEIKKSINKV
                     VKSIESKFAYPVFIKPSECGSSVGISKAKNADSLKKALVFAAMFSRKVLVEEFIEGDE
                     LEVAVLGSYEKISSSGVGQILSSNEFYDYNAKYIDGKSSTLLKPDIPDSSTRQIKKIA
                     EQAFRCVGCYGMARIDFFLTKGKKAIVNEINTIPGFTDISMYPKLWDQSGISYTKLLD
                     KLIKLAEERKEKYIFETDYEGIRYD"
     gene            complement(25549..26043)
                     /locus_tag="PLQ68_01100"
     CDS             complement(25549..26043)
                     /locus_tag="PLQ68_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013841083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HXK71275.1"
                     /translation="MKEHVNYEDIFKRFNEQLPKGVFLNTSFEGEYNTMTIGWGLVGI
                     TWSKPVVCVAVRFSRHTYSLLEKSNKFTISIPKTGTLKKELGICGTKSGRDIDKFETC
                     SFKKDKIIDKCDLHIECEVVQKTAMDKTALESSIKERFYGTDDYHMFYFGEIKDCYYT
                     KEEK"
     gene            complement(26105..27817)
                     /locus_tag="PLQ68_01105"
     CDS             complement(26105..27817)
                     /locus_tag="PLQ68_01105"
                     /inference="COORDINATES: protein motif:HMM:NF012907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HXK71276.1"
                     /translation="MRNLWKYFILTLIILILVSAALYAYSVIFEYIKDKYSPNENIEQ
                     YFSDDQTDLIVDFSRIELDKPVRIINNEILIRYEVIKKYIDPYIYYDEDRGKIIITTS
                     DKVFRLTENSLIAQINKNEMNLDTASQRFDGYLYVPISAFMELWNINAKYISQYDTAI
                     IESYKSYDYTAVVNIPDAVIRKGPSIKEPIYIKDFPQDQMLYVKEVQGEWTIVMTANG
                     VVGFIETKFLNTDFQSKQVVIKYEPKKLFIPDHVVLTWHYVHSSLVTSVPYYADVNVI
                     SPTFFTVIDEKGSIASSASLKYMENAHKRGYTVWPLINNVFSNRDQISAVLADSEARK
                     YVIEQILTYAYIYKFDGINVDFENLYLSDKDNLTQFIRELVPLAHEMNLAVSIDVGIP
                     GGSEGYSLCYDHYELGKAADFVMVMTYDQYWSSHESGGSQAQLSWVETNLLATLKLIP
                     SDKLVLGIPAYTRLWKTDNDGKVTLSRTLTADQVVEIVESKQLSPIWEEERDGFISGQ
                     YYIEYWENGELYRAWIEDDASARLKAQLASKYDLRGVCIWMMSQTNKTVWEAILEGFH
                     SEID"
     gene            complement(27821..28315)
                     /locus_tag="PLQ68_01110"
     CDS             complement(27821..28315)
                     /locus_tag="PLQ68_01110"
                     /inference="COORDINATES: protein motif:HMM:NF040325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6106 family protein"
                     /protein_id="HXK71277.1"
                     /translation="MESWYYEKIVYRKRTGKDDLISLGAVVATLAICIISYYFLKTYS
                     FIIWLGVIYACYRLVASTKFDYEYIFVEDTMTIDKIINKSRRKNIFTLSLKDFEVIAY
                     KNSVHINEYKDNVQQVIDASSSTDDTLFGVAYYKSQRTLVYFESDERVLEFIKKYVQY
                     KFKE"
     gene            28483..28944
                     /gene="ispF"
                     /locus_tag="PLQ68_01115"
     CDS             28483..28944
                     /gene="ispF"
                     /locus_tag="PLQ68_01115"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HXK71278.1"
                     /translation="MKCAIGQDSHRFSKNSQKPLILAGVLIKNHKALDAKSDGDVIYH
                     ALTNAISGITSVNVLGKTADEMCKNGIIDSKFYVLEALKSLQHDILHVSFTVECKTPV
                     ISSYIDNMKENISNLLNIDKKDIGLTATSGEGLTSFGQGDGIMCFCLITVG"
     gene            complement(28927..29613)
                     /gene="ispD"
                     /locus_tag="PLQ68_01120"
     CDS             complement(28927..29613)
                     /gene="ispD"
                     /locus_tag="PLQ68_01120"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:TIGR00453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HXK71279.1"
                     /translation="MEPIRAVIPAGGSSIRMNGINKLVATVAGLPVIVRTIKAFDELE
                     YIEQIVVVVPKEQHELYLKLIKEYKVDSKVKFAYSGLSRRESVFNGLKELSDDEGIVL
                     IHDAARPLVTKKIIDDCIEGAKRYGFCLAASRNTDTIKRADNIMFVENTLDRQRIYNA
                     QTPQAFKTEFALDFHKRAQEENLNVTDDSMIAEYYGHNVKIIESDTLNIKITSKIDLL
                     IAEAFLSNGD"
     gene            complement(29617..30117)
                     /locus_tag="PLQ68_01125"
     CDS             complement(29617..30117)
                     /locus_tag="PLQ68_01125"
                     /inference="COORDINATES: protein motif:HMM:NF014602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HXK71280.1"
                     /translation="MSYSIGDKIVYPMHGAGIIVDKVNKKIMDEIRCYFIVKSPVSEM
                     VAMLPVENMDTIGIRDIMSANEADELLCKIKEMNTVELSNWNKRYRENMEKLRDGTID
                     EIAQIAKSLLTRDKSLSTGERKLMLNAKNAVLSEIMLAKNITLTQAEEALMESINCES
                     EQEGQD"
     gene            complement(30188..31543)
                     /gene="radA"
                     /locus_tag="PLQ68_01130"
     CDS             complement(30188..31543)
                     /gene="radA"
                     /locus_tag="PLQ68_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008977328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HXK71281.1"
                     /translation="MAKNSNYFVCTSCGYETVKWMGRCPSCQEWGTFEEVTELKNLKT
                     DSVREIQLDSVDNIDYSDSHRINSGMTEVDRVLGGGIVEGSVVLVGGDPGIGKSTILL
                     QLCRDVCTDNQILYISGEESISQLGLRAKRIGLKRKNVQLASETDTDVICKAIHDKKP
                     AIAIVDSIQTVYSGQISSIAGSVNQIKNSTLMLVNAAKKSDTALFIVGHVTKEGSIAG
                     PKLLEHMVDTVLYIEGESGSSFRILRGVKNRFGSTNEIGVFEMCDKGLIEVTNPSAFM
                     LEGRNLDAPGTVVMPVLEGSRPLMVEIQALVCPTNFTMPRRMSAGIDQYRVNMLMAVI
                     EKNAKIRLYNYDAYINVAGGIKISETASDLAVITAIASSYKGKKLDSRCVVLGEVGLT
                     GEIRSVNGIDKRINEAVKMGYTKAIIPNVNLKSAMQYENQIEIIGVSAISQAIEEALV
                     K"
     gene            complement(31553..32011)
                     /locus_tag="PLQ68_01135"
     CDS             complement(31553..32011)
                     /locus_tag="PLQ68_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HXK71282.1"
                     /translation="MARLSDVIEDFIKDLIDDTEGEVEIQRNELANYFKCVPSQINYV
                     IATRFNSERGYFVESKRGGGGHITISRMSTDKPYDYFMHIILSMGDEISRHSAEVYID
                     NFMDLNIISESDKKLLKAAVNDNSLKSVPVFLRDKVRADILKNMLMKMII"
     gene            32134..32709
                     /locus_tag="PLQ68_01140"
     CDS             32134..32709
                     /locus_tag="PLQ68_01140"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006627158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HXK71283.1"
                     /translation="MAKLYFRYGAMGSSKSANAIMVVYNYKERNQSAVLLKPQSDIRD
                     GKQINVSRCGLRMDCEFTDSFLDQNDDEFNKYDCIVVDEAQFLTKKQVETLVHIADDL
                     DIPVICYGLRADFQNKLFEGSLYLLSWADTIEEIKTICWCGKKATCNARIGADGKIMR
                     EGPQFLLGGNERYIPLCRKHFRSGQIKKEED"
     gene            complement(32792..34993)
                     /locus_tag="PLQ68_01145"
     CDS             complement(32792..34993)
                     /locus_tag="PLQ68_01145"
                     /inference="COORDINATES: protein motif:HMM:NF036578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HXK71284.1"
                     /translation="MNSINLAKWITADIEPNNDSCRMFQRTIKFEKQVKKAVLNITAL
                     GLYEAFVNGKRVGKDYRFLPGWTHYNRRVQYQSFDVTKNIIEEYADISVYVARGWFSG
                     HIAGDPDKLHFALACSLDVVYADETKDSFISCVNDEWKAAKTSIIFSDIYDGETYDAR
                     LRPYDFKPVRLAQGYNPNDIVLQQGEYVKEHEQLSVVKLIITPKNERVLDFGQNITGY
                     VRFKVKAKKGERFILSHAEVLDNEGNFYIENLRGAKQKIEYISNGGKSEYSPHFSFQG
                     FRYVRLDEWQENVNISDFKAIVVHSDMKRTGYFESSDKMLNKLYENTIWGQKGNFLDI
                     PTDCPQRDERLGWTGDAQVFCKAASYNYDVKKFFSKWLTDLSLCQAMNGAVPSVIPVK
                     YSFVNVSSAWGDAATIVPYQMYLTYNDISILDNQYESMASWVEYIKNSSDNPYLWDTG
                     YQYGDWLALDVPFGTYRGKTNQYLVATIYYANSVDIMKKTAVLLKKKKKVIKYYQDLY
                     DNIKMAFNETYWKDEKLITDTQTSYVLALHFNMLNEERQKKAIANLVERIHDFGDKIS
                     TGFLGTPYILHVLAEYGYADLAYKLLLEKEYPSWMYPITKGATTIWEHWDNIKPDGTF
                     WSKDMNSFNHYAYGSVVDFMYEKVAGIRIDESKPGFSNIILKPVVVKGCGLNYAKASL
                     ITKKGTIISKWEMSKDSITYTFVVPKNTTATICLKETYNVGPGEYTYTENI"
     gene            complement(35197..37269)
                     /gene="fusA"
                     /locus_tag="PLQ68_01150"
     CDS             complement(35197..37269)
                     /gene="fusA"
                     /locus_tag="PLQ68_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004627738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HXK71285.1"
                     /translation="MKTYTTTNIRNIVLMGHGGSGKTTLAEAMLFLTKGIDRFGNVND
                     GNTTCDFDSEEIKRKFSINTAIAPVEWKNHKLNILDTPGYFDFAGEVKCACRAAEIGL
                     ILLSAKNGVEVGVEKSWEYANEVNISRAFFISKLEEENADFFKVCEELRDKFGNSVVP
                     FQFPMIEDGQLKGIVDVITKKAYLLQNKSVIEAEIPSQYVDKAEELREPLIEAVAETD
                     EELMEKFFEGELFTDEEIVKGLIHGIEQDSIFPIFCGSSMMHVGMDTLMDALVAYFPS
                     PEKYKIHAVKAGTSDEVVLNPSADEKFSGLVFKTMVDAFVGKISLVKVCSGELKSDMT
                     VYNSRISDTEKISNPFVLRGKKQEQCEKAIAGDIIAVAKLSDTQTNDTLCSQLNPVEL
                     KKVVFPEASISLAVEPLAKGDEDKIGSGLNKLRDEDPTFRVENNSETKQLLVSGLGEQ
                     HIDVITSKLKSKYGVSVKLTDPKIPYRETIKKQLESEGKHKKQSGGHGQYGHVKIVFE
                     PGETEDLVFEEKIFGGSVPKNYIPAVEKGLRECVVKGVLAGYPMVKLKATLIDGSYHD
                     VDSSEMAFKIAASLAYKKLIDATPVLLEPIVSCEVLIPDSYMGDIIGDMNKRRGRILG
                     MNPAENGYQKVLAEVPHAEMFKYATDLRSMTQGRGSFTMKLERYEEMPSNLAEKVVAA
                     AKREAEEE"
     gene            complement(37270..37911)
                     /locus_tag="PLQ68_01155"
     CDS             complement(37270..37911)
                     /locus_tag="PLQ68_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015926244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HXK71286.1"
                     /translation="MQAKKRISEAVIRRLPRYLRYIGELKDNGIEKVSSSQLGEKMGM
                     TASQIRQDFSYFGGFGQQGYGYSVQLLFDSMKQILLKDQGFTVIIIGAGNLGTALAKY
                     NNFHDRGFDIIGIFDINPKLIGKTINSNEIMCIDKLEDFISEHHPDIAILSLPRSETK
                     DIAVRVANSGVKGLFNFSPMDLKLPHDIPIENIHLSDSLMVLGYKIKNFQEEK"
     gene            complement(37999..38796)
                     /locus_tag="PLQ68_01160"
     CDS             complement(37999..38796)
                     /locus_tag="PLQ68_01160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK71287.1"
                     /translation="MNIDVFDKYNKEIQKECEAKLDYVTTKAEYISLINDARKHFETT
                     SIQGSPINDVIQTQIEESGIHEVLNNALMVCDMYLPYSLIDLMQKKIGFEGIKKMIDD
                     KSKPEADRASLINSLIGIENEDLYKYLIDIIVTSDSELLKEEAADVLSLMDKNKVNLN
                     ILESFDKNGVNNELLSVLVAVYKDTDESDSIYQLLRKHFILSDNKSMIANIMADLNDG
                     RAVVFLRGYLSKNLNDIAKSEIVDICSAISRLGGYTEDFIKFNNQTH"
     gene            complement(38793..39323)
                     /locus_tag="PLQ68_01165"
     CDS             complement(38793..39323)
                     /locus_tag="PLQ68_01165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK71288.1"
                     /translation="MKLFLKHAGKILFAYSTAAAVFTFFVIAPLSMANNMTTWLLLYS
                     FVMFMFMFFMVSKTALSVGKYERYDKDAKAHPAKGLLYGLAAMTPYLLLGLIHLLIFD
                     NSLELGLRIFHYIFRCALGPFYFMINTLGYTWYAFAIAYMVVPVISGIWYFMGLKGID
                     KLRVNKSIKDDEAFLK"
     gene            complement(39330..41045)
                     /locus_tag="PLQ68_01170"
     CDS             complement(39330..41045)
                     /locus_tag="PLQ68_01170"
                     /inference="COORDINATES: protein motif:HMM:NF025598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HXK71289.1"
                     /translation="MKNLKTIIIIVIILAVAIATYFIVDDIISKTRVINPNINEVITP
                     SNIKSSDIVRYSFSISGEQTTVELMEVTVRDDEGNERSEMQYRLVNEPDKELNNKIET
                     ALVQAASLISVNLIEENPTDLSKYGIDYNSFFEVTLKDGTSYKVYFGNVIDVTYNVYV
                     MREGVDKIYTISDTSFGMLTIYREYLLSEVIFPGDANTIASFSLLKKGELEFSIEPDQ
                     YVKWVLTEPLGSRVYTQTAQEMIDTTYEMLIGEYVNVMPSEDDYQNYGLLNPEYSIKV
                     TAENKTVLLHIGKELIDKNSFYAKFSNSDEVFLVDKKYLGWIDTELMEILYPIPYEPL
                     IKNVKSMHYVFASGEVYDVLIEEQEYIIDDKPAKNYEYTINGDIVDLQYGSDLYNTTF
                     YGTAIVSYDNEWQGPDESQKPFLLIEMNYVSGLNEYIVYYTKDEISMYYIRYNPEYDM
                     MSQYTGAVVDSGPIEGFFNQFLVQYTDYLYVTCIDGKEHEFGDWIVVKEATEEEEGLK
                     EKVCTVCGHKISAPISVIKHTDPGSYRGANWWIILIIVLAGGAIGATILVNRAKSKKK
                     EEEKQ"
     gene            complement(41046..42500)
                     /locus_tag="PLQ68_01175"
     CDS             complement(41046..42500)
                     /locus_tag="PLQ68_01175"
                     /inference="COORDINATES: protein motif:HMM:NF021351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GldG family protein"
                     /protein_id="HXK71290.1"
                     /translation="MKKDNKFLNIFKSKNFRYGTSSFIIVAVVIALFVVINITVPLLN
                     IEWDLTPEGLYTLGDTSKTILDSLEDEVEIIGLMDPTKINSSSSFFGVIKFLDYYDAY
                     DNINVTYVDPDVNVGYVSQLDPTGALDLSRQSFVVRRVKDGNIRAVKYYDLFSSFVSS
                     ESTFQITDTGSKVENAFTSAISYVTRETYTTAYFVLGHNEYSYLDGYITARDVMELNG
                     YNVSTFDMRADMAIPEDADILLMINPTIDFLDEEIKLLENYMKNGGRLMIALGSAETR
                     EEYPNIQKFLDYYNVQYNYDRIKEYNSDSYMASNQYYIFPTIVGTEATLNVYDNISYL
                     LMPNTRSIRILNKSKSFLEVNPILKSSSISKQESSISKYESTKGTAYAGAAVKSLSTD
                     SRLIVLGSSDFMRDYILVNNKAYEPDSTKFFMGLVNWLEGDYTQVRIEEKNYFVNVLS
                     ISASQANFVAVLLYILPALILLVGGIVYLKRRHL"
     gene            complement(42506..43210)
                     /locus_tag="PLQ68_01180"
     CDS             complement(42506..43210)
                     /locus_tag="PLQ68_01180"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXK71291.1"
                     /translation="MSAVARKEIAGYFKSPIAYVLIGVYILIISIFFHPALNAGYGDF
                     VEVLDDVGVFLIFIIPILTMRILAEDKKNGTEVLLLTSPTSLPRIVIGKFLATFTVFL
                     VIVGISFIYPIVVVLFEGAITTQLVGAYIGFILLGAAFCSIGVFCSSLTENQIISAII
                     SLVILFGMWIADQFAATVGGLAGAIMEWISVLTRYGVFTKGLLTMENIIFFVSFTAMM
                     LYMTVRVIERRRWVQG"
     gene            complement(43212..44171)
                     /locus_tag="PLQ68_01185"
     CDS             complement(43212..44171)
                     /locus_tag="PLQ68_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014253977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXK71292.1"
                     /translation="MIEIKNLVKKYGQVVAVNDISFKVKEGEILGFLGPNGAGKSTTM
                     NIITGYLPSTSGTVEVDGYDITTHPEQVKKRIGYLPEQPPLYKDMTVTEYLNFVSDLK
                     KAPRNKRKSELSDIMELVGLTDHRKRLIDNLSKGYRQRVGLAQALIGNPKVLILDEPT
                     VGLDPKQIVEIRRVIKGLASDRTIILSTHILPEVSMICERVVIINKGQIAAEDTPDRL
                     SNNLSGVSKLNLTVKGDKDKVLKTIGNIPNIKYITMTTKSEESVYKYVLETKNNVDIR
                     EDLFYRMAENKMPIIELKPSAATLEDIFISVVSNAGITQTEEE"
     gene            44377..44772
                     /locus_tag="PLQ68_01190"
     CDS             44377..44772
                     /locus_tag="PLQ68_01190"
                     /inference="COORDINATES: protein motif:HMM:NF025384.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4234 domain-containing protein"
                     /protein_id="HXK71293.1"
                     /translation="MYDGEHRPLILIILFSIISVGFFNFYWLYIATYEIKQVAREPEN
                     IKPGVEVFLAIITAGLYIIYWSYKYNKLLADAQSMHKVTPQDVSLVSSIFSFPIITFI
                     GMCFTQHQLNRLWKYGTAPEQKKKKSKKR"
     gene            complement(44846..45192)
                     /gene="rnpB"
                     /locus_tag="PLQ68_01195"
     ncRNA           complement(44846..45192)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PLQ68_01195"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(45227..45970)
                     /locus_tag="PLQ68_01200"
     CDS             complement(45227..45970)
                     /locus_tag="PLQ68_01200"
                     /inference="COORDINATES: protein motif:HMM:TIGR00486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HXK71294.1"
                     /translation="MKKVKIIEFINSIAPFEKSIADDNIGELIKGKDNIRRVMICLDI
                     SKEAIEFASSSKVDLIVTHHPVIYNPLFRIDDDVILAAIRNKIGIISAHTNYDAARLN
                     DVLAEKCEVSVIGNIFLEDGIYLGRIGNTAKISFYDYLDKIKTNLKVDTVKITGKIPH
                     DVEKVAIGTGSSSSFIEEIKYLDVDVFITGEIKYDNLKSLAFEKPAVIELGHYESEEC
                     FIDDLSNLLSKRFPKLDVITYKKRISIYI"
     gene            complement(45948..46643)
                     /locus_tag="PLQ68_01205"
     CDS             complement(45948..46643)
                     /locus_tag="PLQ68_01205"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016689.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HXK71295.1"
                     /translation="MKDIESLLSPRMMMVSRCVKPCEVVYDIGTDHCYIPIYLIQKKV
                     CKKAVATDIRQGPIDKALENIRYFNLSEEIKAFVSDGISHVGEKANIVISGMGADVII
                     NILSDHMDYAKKAEQLVIQCQSRTEHLRSFLWENGFSINKEDLILERAKVYNAFSANY
                     IGKVESFTHTEAVLSKYLISSKHPLLQNYIIKHLKKIEDMIKGMKKSNQSYHQLTLIK
                     KEMEKIHEESKNY"
     gene            46810..46886
                     /locus_tag="PLQ68_01210"
     tRNA            46810..46886
                     /locus_tag="PLQ68_01210"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:46844..46846,aa:Ile,seq:cat)
     gene            complement(46950..48647)
                     /locus_tag="PLQ68_01215"
     CDS             complement(46950..48647)
                     /locus_tag="PLQ68_01215"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF014156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HXK71296.1"
                     /translation="MYNNCLKVVYSHGNNGKHIVHEFNEPGVFNDCELEIIKSGNSST
                     YIIRFPDGVTLHHVSIEKHYNFSKNSRVFCNGYQSWSVSREYEIDEKSENLALLGHLI
                     NKHYSLDRYNGQDKIKAKGKKGNFLSYTYTYIKEDKNSSNIMLLGSLSERSGFTIYQH
                     DANKNAIVIEKDCEGLENVNKYMFSLYEDEGGYDGVFDRYFDNCRQKKTGSAIRSSYL
                     DGIDRKYKGFTTWYNYYQEISQRKVQKNLYYYTEKNPSCDIFQIDDGYQNFVGDWLDV
                     NIEKFPIGMKKIASKISGKNVMPGLWLAPFSAEKDSKLFKEHPDWIVKDAKGKPYKAG
                     SNWSGFYALDIDNRNVKNYLEIVFDTVIKEWGFTFLKLDFLYSACILPRNNKTRGQLM
                     CEAMDFINDICKDAILLGCGVPLGPAFWNVDFCRIGADISLEWDNKRYMQLAVRERVS
                     TRNSVVNTVFRKHLDKRVFLNDPDVFMIRSQNCFMDYTMKYILGNINSAYGSLLFTSD
                     EVEEYDEQQDELLYQIIKGMPKASKEYVENKCLVMEFSDKKIIIPLENNKKPVLTYL"
     gene            48765..49283
                     /locus_tag="PLQ68_01220"
     CDS             48765..49283
                     /locus_tag="PLQ68_01220"
                     /inference="COORDINATES: protein motif:HMM:NF016763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HXK71297.1"
                     /translation="MDKIKGYFTSNARVLISWSLFIIWMIVIFILSAQNAEKSSDTSG
                     SVLTFILNITYRGFKDMNPSEQFELLEIYQGFIRKFAHFFIFGILGVLAVNAYKSLKI
                     RDNGKVAILAFATCVIYAITDEVHQLFVPGRAFEFTDIIIDSSGSFIFISLSLLIVYL
                     IENRKEAQSDMP"
     gene            complement(49372..50499)
                     /gene="rpoD"
                     /locus_tag="PLQ68_01225"
     CDS             complement(49372..50499)
                     /gene="rpoD"
                     /locus_tag="PLQ68_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003358052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HXK71298.1"
                     /translation="MEEKANTAKKEKLQAVEEEVTPLTFDKVMQDLMEKGKAKGSLIS
                     QEITDSFDEIEVNADQMDKLFEMFEEAGIDVVDDEKDISEVDYETEDIEITVPDGVSV
                     DDPVRMYLKEIGKVPLLTVDEEVALAKGMEAGDEECKRQLAEANLRLVVSIAKRYVGR
                     GLQFLDLIQEGNLGLIKAVDKFDYTKGYKFSTYATWWIRQAITRSIADQARTIRIPVH
                     MVETINKYTRVSRQLVHELGRDPTPEEIAREMNMTEDKVREIMKINQDPVSLETPIGE
                     EEDSHLGDFIPDEDALAPADAAAFTLLKEQLMTVLDTLTPREKKVLKLRFGLDDGRAR
                     TLEEVGKEFNVTRERIRQIEAKALRKLRHPSRSKKLRDYLE"
     gene            complement(50511..52340)
                     /gene="dnaG"
                     /locus_tag="PLQ68_01230"
     CDS             complement(50511..52340)
                     /gene="dnaG"
                     /locus_tag="PLQ68_01230"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HXK71299.1"
                     /translation="MADRYIYPEPLIEEIISKNDIVDTISRYVKIDRKGKDYFGLCPF
                     HKEKTPSFSVVPAKQIYYCFGCNKGGNVINFIKDIENLDYAEAVRLLAERANINIPQK
                     NSYVDEATLKKEKRTYEMNSIAARFFFDCLKSKENPEPLQYLINRKIEKDTIVSFGIG
                     YAPNDWTKLTEFLKKKGFSEAEIIEAGLGSKSKNGTIDKFRNRIMFPIIDIRNRVIGF
                     GGRALDDKDVKYMNSPETCVYKKSKSLFSMNFAKNSREDYVIIVEGYMDCISLHQAGF
                     TNTIASLGTALTDNQAKLIKRIFGQAVIAYDNDSAGQNASLRGLDMLANMGCVVKVLV
                     LPKEDKHGRSIKDPDDYIKYKGKDEFKQAIKKSLMLVEYKAQLLRSSCDLNTSEGQIA
                     FLKGVASAIGQVESPVERDVYIKNISARFNTSYDALLKEVQNRTKDDSDTQYNQIKAK
                     PVTKIASSLRDRYTEKKLDKIFYSILVLLCNNPGFKDEIRKELIEAGMTHNDFSKELL
                     EEILNKYDSMESPDEAKISAMLDDEQRGVFIKEAAEYHTISDPNKALSDYIARYRHDI
                     IVNNNIVLSKALVSEGVTEEEKEKLKDELRRNTAMLGKYKISG"
     gene            52449..52730
                     /locus_tag="PLQ68_01235"
     CDS             52449..52730
                     /locus_tag="PLQ68_01235"
                     /inference="COORDINATES: protein motif:HMM:NF039477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6514 family protein"
                     /protein_id="HXK71300.1"
                     /translation="MTSYKLKRSSRLADEEKNILDIDYFIIEEFKIRTNSKSYGIKVE
                     TKNKGQITDTCTVNDLTANLDEAMYIISKMADNNVSAIAAKDVIEDMVY"
     gene            complement(52724..53971)
                     /locus_tag="PLQ68_01240"
     CDS             complement(52724..53971)
                     /locus_tag="PLQ68_01240"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HXK71301.1"
                     /translation="MIEIEKLSNGIRVIFEKLPAKNIISIGVFVKAGSNYENEDNKGI
                     SHFIEHMTFKGTKSKSAKNIAIISDQIGGMLNAYTGKDMTCFYIKTTSDNLSVALELI
                     SDMLLQSKFDELMIENEKKVVMEEIAMYEDSPEDYVHDRLEEEMWKGNPLSFPILGSI
                     ETVSKYSYDTIVSYMKEFYVSCNTVISVSGSFDKEVILDMLENYFSDYPQGECHKANN
                     GGLAVFRPVNIFKAKKIEQAYLSMAFKGALHDSDERYPFAVLSGALGQSMSSRLFQIV
                     REEKALVYNIGSSNEFYQAGGILNIYAGCNPSRFDEMMECIKDIIYDICSNNLDSEEI
                     TRARDQIKTGMLLGMETTDSRMMSNGRNLLLRGRPITREEAIKKTEKVTAEDILDIVN
                     KYISWDNKAVSVLRPNKPRKKIQ"
     gene            complement(54015..>54713)
                     /locus_tag="PLQ68_01245"
     CDS             complement(54015..>54713)
                     /locus_tag="PLQ68_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015925173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HXK71302.1"
                     /translation="VTDEEDPDNFEMMMDIQGIEDFFGDMDFKVAGTHKGITAIQVDI
                     KVDGLTPKIIKKAFEITQKGRIYILDEVMLKVIDKPRPELSPYAPKIITMTINPEKIK
                     DVIGTGGKVINRIIDMTGVKIDINDDGTVYILTVDQESGDKAVSIINGIVNDLEAGMI
                     FNDAVVTRIMEFGAFVELVPGKEGLVHISQIANERVNKVSDVLSVGDKIDVKVVEIDR
                     QGRVNLSRKALLSK"
     assembly_gap    54714..54813
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<54814..55985)
                     /locus_tag="PLQ68_01250"
     CDS             complement(<54814..55985)
                     /locus_tag="PLQ68_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HXK71303.1"
                     /translation="MYENHKKHVINYAGKELIIETGKHAQLANGSCLVRYGDTVVLST
                     ITASKEPRPGIDFFPLSIDYEEKMYSIGRIPGSRNRREGRPSDAAILVSRVIDRPMRP
                     LFPHDLRNDVVIICTALSVEQDNQPQLAAMIGASIATSISDIPFNGPVAAVALGLVNG
                     EIIINPDAAQRAVSDMYVTLAGTKEKICMIEAEAKQVSDDIMYDALMKGHEEIKKVCE
                     QIEKIVSEIGKEKFSYTSSAVPQEVYDEVAGIANEEMKQAVLAVDKTVRDTKISELTA
                     KVNEQLGEKYKDNPSYIADSLYKLEKKVVRHYLLHEKKRVDGRSLDEIRPLYSEVGVL
                     PRTHGSAMFQRGQTQVLTSVTLGGMREVLPLEILDEAETEDRYIHHYNFPAYSVGEA"
     gene            complement(56128..56391)
                     /gene="rpsO"
                     /locus_tag="PLQ68_01255"
     CDS             complement(56128..56391)
                     /gene="rpsO"
                     /locus_tag="PLQ68_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012101825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HXK71304.1"
                     /translation="MEKEKKQGIIQKFQLHEGDTGSPEVQIAILTERINHLNEHLKSH
                     KKDHHSRRGLLMMVGQRRGLLNYLMKEDIERYRTVVKELGLRK"
     gene            complement(56598..56855)
                     /gene="rpsR"
                     /locus_tag="PLQ68_01260"
     CDS             complement(56598..56855)
                     /gene="rpsR"
                     /locus_tag="PLQ68_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013486352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HXK71305.1"
                     /translation="MDRMDREDRKDFKYRKPKKKVCQFCADKDESIDYKDINKLKRYV
                     SERGKILPRRVTGTCSRHQRELTIAIKRARQIALLPYVADK"
     gene            complement(56885..57298)
                     /locus_tag="PLQ68_01265"
     CDS             complement(56885..57298)
                     /locus_tag="PLQ68_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014117853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HXK71306.1"
                     /translation="MNKVILMGRLTKDPELRTTSNGTPVASFSLAVNRRFAAKDSQVQ
                     ADFFNITAWSKQAEFVNRYFRKGQQVVVIGRLQNRSWEDQEGKKRYATDVVLDETYFA
                     DGKKDSNDSDNTGNNAGDDMDFTHVDISEDDALPF"
     gene            complement(57308..57589)
                     /gene="rpsF"
                     /locus_tag="PLQ68_01270"
     CDS             complement(57308..57589)
                     /gene="rpsF"
                     /locus_tag="PLQ68_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014311766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HXK71307.1"
                     /translation="MNQYETIFIVDCTLEEEAQKNTVEKLIQQMTDAGCEIDKVDEWG
                     KKKLAYPINYKTDGYFVLVTFKAQPDFISELERLYRITEGIIKGQTIKL"
     gene            complement(57750..58709)
                     /locus_tag="PLQ68_01275"
     CDS             complement(57750..58709)
                     /locus_tag="PLQ68_01275"
                     /inference="COORDINATES: protein motif:HMM:NF013408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I mannose-6-phosphate isomerase"
                     /protein_id="HXK71308.1"
                     /translation="MLYPLKFKPVYKDYIWGGNNFLKLNKKLEYPITAESWEISANKD
                     GMGIVSNGDLKDATLENVFRWYPKDLCGTASQEQFPLLIKFIDACSLLSVQVHPDDLY
                     AYENEDGALGKNEMWYIVDAKPDAKLVYGLKKGVTKEIFADAVQNNRIEETLNFVNVK
                     KGDWLNIPSGLVHAIGEGIIIIEVQQNSNTTYRVYDYDRIGPDGKKRPLHIEKAMDVI
                     NFNNPEIQINKTVQFKGVDYLVYNKYFAVEKIVSNLQYSSDTQYSSFHTYTVIEGEFE
                     VDGVRVGLGESVLIPASMEKYTLKGGFTAIKSFVPDISRRAVC"
BASE COUNT        18455 a        10939 c         8761 g        20496 t
ORIGIN      
        1 atgagcataa aattaagccc gtgaatacga cacaaatgga atttagtctt tcattatttt
       61 tcgacttgct taatactgga atttaagttt tcactttacc tattaatgat attaaatatc
      121 aaaacattta agcgtttaat actcagtaaa atttgactgc ttaatattta tactgttaac
      181 aaattcagaa ttattcttgg ttttaacaag tttatcaatc atccattcag taacttctgc
      241 agtacccata ttgctcatag cacgtcttat tgtccaaatg ctttcaagtt ctttcttgtt
      301 cagaagcaat tcttctcttc tggttcctga cttatttata tctattgcag ggaatattct
      361 cttctcagac aattttctgt caagatggag ctccatatta ccagttccct tgaattcctc
      421 ataaatgaca tcgtccatcc tgctaccagt ctcaacaagt gcagtagcaa taattgtaag
      481 gcttccgcca aattctatat ttctcgcaga accgaagaat ttctttggag catgtaatgc
      541 accaggatca agacctccag ataatgttct tcctgtcgga gtcattgtaa ggttatgagc
      601 tcttgcaagt cttgttatac tgtcaagaag aattacaaca tctttaccat gttcgacaag
      661 tctttgtgct ctttctaaaa ccatttcagc aacttttaca tgattctcag gaactttatc
      721 aaaagttgaa aatactattt ctccatttac tgatctttgc atatcagtaa cttcctcagg
      781 cctctcatca atcaaaagaa caattaactc gatattagga tgattaattg taatactgtt
      841 tgcaattttc tttaacaaga cagttttacc agcttttgga ggtgatacta tcattcccct
      901 ctgccctttt cctattggag caataagatc aataatcctt gtcgaataat ctttcggact
      961 attttccaaa gtcagtcttt cttcagggaa gataggagtc agagtttcaa aaggttttct
     1021 tttaaaaacc tgtgaaggat catcatcatt aatctttaac actgtttgca atgcctgaaa
     1081 tttttctgtt tctttcttag gcttccctgt tccgataatc ttgtcgcctg ttctgacacc
     1141 atatttccta atctggttag gagcaacata tatatctttt gaaccatatg aatagttatc
     1201 agtacgcaaa aatccataac catcaggtaa cacttcaagt acaccgctta cttcatcgta
     1261 ctgttcacta tcaaactgtt cagccatatc aggttctgaa acctgagaga actcttttac
     1321 ctggtctgct tctttgcctt ttttttctgg caaagcaatt tcagactcat catgtgcttt
     1381 aattacatga cgtgtccttt tctcttcttt aaccggtttt ccagattctg attcttttgc
     1441 catctttgac aatagatatc ttggcttctt tggtgcatct tgagtaacgg tctctaatat
     1501 tttcgttact tcaacaactg gagcctcttg tttttcttcc ggttcttctt tgtttgtagc
     1561 ttcgggtttg gttatctcca aaattctttt aattatgtca tcttttttca taatcgtaac
     1621 gctctttata ccgagcgttt ttgcaataac ccgcaaatcc gcaatggatt tcgaccttaa
     1681 aatctcgtcc tcgttcatta aaatcctcac taaagttttt ccttgatatt ataacaaata
     1741 atagattatt atttatcgag tatagacatt atcacataaa tgataccttg tcaaacattt
     1801 ttttaaacaa ttatttctcc aacattatta tagatatact tcggcctata tgaaaagttg
     1861 agtatattat cttcatttgt aacaccgctt aagactaaaa ctgtatctat ttcactttct
     1921 atcccggcaa caatgtctgt atccatacgg tcaccaatta taagggtgct ttttacatct
     1981 gttccgagtt tcttcaaagc atgtctcatc attagtggat taggttttcc tatgtaataa
     2041 gcatttttac ctgtagctat ttctattgga acagctaaag caccggtggc aggcataatc
     2101 cctttttcag taggtcccgt tttgtcagga tttgttccta ttagttttgc tcccttttga
     2161 ataagctgga cggctttctc tatcttctca aagctgtatg cccttgtctc accaataatt
     2221 acatactcag gactcatgtc attcatagta attccaacat catataaagc atttatcaat
     2281 ccaggttccc ctataatata tgcagaacac tttggcattt gtgtagataa aaatattgca
     2341 gttgcaatgg ctgaagtata aaaatgttct tctgctacct caataccaag cctagctaac
     2401 ttctgtgaaa gctcttttgg agatctttca gaactattag ttaagaatag atatttttta
     2461 ttattagcct tcagccagtc tacaaattca agagcaccct caagaacatt gttaccatga
     2521 tataaaacac catccatatc tattataaat ccttctttat tctttataac ttcattcatt
     2581 ttccactcct atttgttttc ttattctttt ccttctccat aatatcaaat aaatgaatag
     2641 gttagatatg agatattctg ctgtagtatt tgaattaatt gacagattat cccgatcaat
     2701 caaaacctgc ccaagaaata gtttctgtac tgctttttta ttaattctaa ctttattttt
     2761 gtcccatatt tttccccaaa taaacttgaa aatatttttc tttgattttt caaactcttt
     2821 tttttctgtt tgaacagaac ggtaagcaat aagaattttt gtaataagct gttctggagt
     2881 atatgcacgt atcttttcaa taccaaaaat atcagcagtt atctccattg cggcaaaaaa
     2941 agcttttttt gcatcaagag taccatcaga atgattacgt aaggttctaa ggatagaaaa
     3001 ctgaacgacc ttattcatta tgttattctt attctttttg atattcagat aaagcatttc
     3061 aatttccttt tctgtcaaag ccattgcata tttgcttttt gcattatctg atatgtaata
     3121 acacttaccg atatatttac cgaatgcttt caatccatca agatagccaa gtaacatatt
     3181 gcgtttagca atttctggat caaattccaa aatgcctcct tcagttcttt cattctctat
     3241 aataattatc tttgcatctg attcttttac ttttctatac atcccaatag ctgaattgct
     3301 aactgcgatt atattcttat acccgttatc tataaggaca tttatcggca tattatcata
     3361 tatgccacca tctatgtata agtctccatc tatctcctgc tttttgaaac caggaactgc
     3421 tgaactggct aatataaaat ctacaacttt accttgtggt atatctttct tgaaaagtat
     3481 gacaggtttc atctgtgtaa gtgaaaatgt aactaatgca taatcaattt ctgatgctcg
     3541 taaaacatct tcatcaatta ctttattaag atactctctt agtgtagtag tatctaatcc
     3601 tttatttttt agtatcgatt cgagatactt agatgtatta ggctctccat cttctttgtt
     3661 ataagttata ttaaacaatg tcggcatatc aagcgtctgc cataaatgct ctgcctggtc
     3721 gaaagcatca cacacaacag cagcaccgtt aatagatccg actgaagttc caatgaccat
     3781 atcaatctca atattcagtt ctcttaaggc cttccaaacg cctatctgat aagagcctct
     3841 ggctccgccg cctgctagca ctagttggta tttcaattta gttacctgcc tatattgata
     3901 ttgattatat ataaaagata aattatttac aatgctaatc agtttgaagc gccttggaat
     3961 ttttttattt ccagttcaat atctaatgaa tctgcgtatc tagtcatttc atctatctgt
     4021 tttctctgtc tattattaag tgcttcattt ggtattacaa caagaacctt cttcttttct
     4081 atatcagttt catctatagt aacatttctc aatgactgaa tcgtgtagct gtcaatagca
     4141 tcaatctgct gtttgatttt cttcttcata tctgtacctg ataaataact ggaatcttga
     4201 gtgtttatac ttattatcga tactgcttcc ctactgtatt catcatacat atccaacact
     4261 ggaaaatctt tctcaagatt agctcccata atctgatgaa taacatcacc tctttcaaag
     4321 ggactaagat cccaaatatt acattctgca gtaccaatcc catcaccttt ccacttgggt
     4381 gatattgctt ttatgtgcat attattatta gcaagcactc cagtataaat agaccttgct
     4441 gacatagtaa aaaagtattc atatggcaaa gccattgttc ttatattttt cacacactca
     4501 tatctgtctt tttttagagg atttaataga tatatatcag aaatatgata agtaacagct
     4561 gcatttctta attcacattc caaatcctgt aaaatcgtaa aattcgcttt tgcatagcct
     4621 attaactgta tgctcaataa aagtgcaaat acaatcacta ctgaacatat acagcattca
     4681 gcagttatac ttcctttagt atgcatcaga cacctcgaat ttgtatatcc ttttaattcc
     4741 aaatatacct tcataagata aaacagcttt aacatttatt ccacagtaaa cctgtgacat
     4801 gtctatcaca taaggacagt taattgatat taaatcaagc agctttttag ttttttgatc
     4861 atttgatgta gtgcacataa gaactcttat ataatcctca taatccattt ttaaccagtc
     4921 tggtgttttg ttaaatccag aatcaattcc aatatcaatg ttccaatcac catcaagctt
     4981 aaaaagagga catttttcac ctcttttcaa agtattagta tcatatgcag cttcagcgaa
     5041 agcccaagaa cataaaatga ggtttttagc tataggagtt gcagcaccaa aacttaagct
     5101 acctgcaagt gatgcagcta aaacatttgc ttttgtagtt ttgtcaatat cagtatagac
     5161 atgcattatg tttaaagcaa agcgtaatgc agtaatagct gcttcacatg atttaatatt
     5221 tttactattg tctcgattcc caaagataat atattctgtt tcccctttaa gatatttact
     5281 ttttgaaacg tttttttcac tatctgtaat actatttaga taaagcatta agtactcatt
     5341 gaaataaata tcttcttcga gttttgataa catactgcat atatcatcaa tggacaaaaa
     5401 tatgcttttc gatgtattac cagtcatgtc tgcttcaatc gtaaagtcta aaaattcgtc
     5461 atctgatatt aaagctataa atgaatcaat tgtgttatca tcaaaatcta atgagttcaa
     5521 catattcttt gcatactctt tccagaaccc tcttttgtcc tcgttattat cttcgaacag
     5581 ggtgaaaaaa aaggaatcca gtagctcagg attataaaca acatcatagt attcctccat
     5641 tgcatcattg aaaacatata atataggttg ttcacttcct gaaattactg cttcagtaat
     5701 atctttcaaa gcattttcca aacattttaa atttctgtca aaggttcttt caatctgatc
     5761 aaaaactgta tttgaaaaag aattttccat actgtaaact gtatcgatac aaatttcaag
     5821 aaaaacttca agatattccg gacaatcttt tattgaactt acaatacgtc tcatactatc
     5881 agataaaata tgaacttgag cataagtatg aaccatctga cttattagat tcaaaccctg
     5941 tttgttatat tcttttaaca catatacaag attatcgata ttgatattta taatttccaa
     6001 agcttcttcc ttaagtaatt cgatagcatt tacattgtca atattttcag cattatctat
     6061 taagtcatgg atattatgaa caatataatc tacactttca atcatataaa taagatcctg
     6121 ataatatgaa ctggaattta tttgctcaat agttgtttta agtttttcat gaacattctt
     6181 aagtcctcca acaagctcat caacctgcat ttttatattc aatgtcttta aaacatcttt
     6241 catttttttg aagatatcaa ttttctcatt tacctcaatt aaaatatctt tataaatgtc
     6301 atctttcatc aatgtaaaaa cctgtgaaga taatacactg taatcgtata tggggtttgt
     6361 tttagtgact tctatatttt gaatatccat tttataaatg cccgtactga tacttgattt
     6421 gtccaatgct ttaaaaacta tatcataagc tttaccatca tcaatcgtat atccatataa
     6481 tccatattca ttttgcataa aagagctata ctgagacaat acacagccac atgaagcttt
     6541 aagcgcattt tttgctgcat cagatgcatt ctttgcaatg tagatatctg aaagaactaa
     6601 agaaagtatt agaatcactg gtaaaacaat tgcaacaaat actgatatta tacctttttt
     6661 attattccca taatgcttca aaaaaaccat ccgccttcct ttttaaactg tccgcttttg
     6721 aaatatcttc tgtaaggtta tattcctcaa tctgttcata cttactactt attgaagtta
     6781 ttgaaaagat aactacaatt gaaagcaaaa ttataaatgg caatgctata gccgcttctg
     6841 tacatgcgta tcctttttta cttgttgata atgaagcatc catgagtcaa taataggtgt
     6901 attttcagta ttatttaact gacaaacgaa attaattaaa aaaatgtatt cactttatga
     6961 ctttaaagta tataattaaa aaaaacaatt aaataatgga ggtaacggtt ttgtttgatc
     7021 ctaattctaa tctgatgtac ataatggtcg ctataattat tctatatgta gccgctcagg
     7081 cagtatattt tttaattaaa gcagtaaaac gggcaaaaga attgaatatt agcacttcaa
     7141 ctgttaagaa aacaatacta tctagtgcag tcttcactat tgcacctgct atttctatac
     7201 ttatgggtgt tcttacctta gcaggtttta tgggattgcc tttcccttgg cttcgcctga
     7261 gcgtattagg tgctataaca tatgagcttt cggctgcata ttccgctgct gaatcgctgg
     7321 gcatagaact gacttcactg gtaacagatg ccaaagtatt ttctactatg gcatgggtta
     7381 tgcatcttgg tatatttaca ggccttatac ttgtgccatt atttcttaaa aagatacaaa
     7441 aaggccttgt actcattaaa agaaaagatg aaaaatggtc tgatgcttta ttgacagcac
     7501 tatttctcgg aatgatatct gcatttttag ggctagtatt ttctgatatt aaatcaggtt
     7561 tagaaggatg gatacctgta tttgtaatga tagtatccgc tctaataatg tctgtgatag
     7621 gaatactggt taaaaaattg aaaattaagt ggctggaaga ttttgctctt ccaattacta
     7681 tgttgtcatc aatggcgttg gcaataccga taacaaatct tgttcagagc atattatagg
     7741 aggaagaaat gaaaaagaaa acaattagat catatgatga ccaaatacat ttttcaggaa
     7801 gattatggat gcttggagca cttgtattat ttacaatgct tcctttgaca atatcagtat
     7861 tgtatgatgc atggccttca gcaggccctt tcttcaaagc attagcaggt gtagcaccaa
     7921 ttttttggac agtaggactt attgaaatta taacatacac ccctatgcta ggttcagcag
     7981 gatcatattt atcattcgta acaggaaaca tatccaactt gaaagcacct gttgctctta
     8041 atgctatgaa ttcagaaaat gtgaaaccag gtactgatga aggagaagtc atatctgcga
     8101 tatcaatagc tgtatccagc cttgtaacca tagtcataat tgcgattggg gtattaggat
     8161 tgagtgcgat aaggccgttg cttgaatcag aaactttaaa accggcattt gcaaatattc
     8221 tgcctgcttt attcggaggg cttggagtag tttacgtatc aaagaactgg aagatagcat
     8281 tagctccatt aatatttatg ttaatactgt tcattgtatc tcccattgat ttatcaggtt
     8341 atgtcggtat attagttcct gttggagcaa taatagcaat tgtatctgca aggatattat
     8401 ataaaaagaa taaaatttag gaggatgaca tgtggtggtt agtatttcca gcagtattgc
     8461 tggcattcgt tattttcatt gtcataagag cgtttttatt taaaccttat gattcacaag
     8521 caccaaaaac acaggaattt gaaatagatg aagaaaatat agtaaacagg ttcagcaaaa
     8581 ttataagctg caaaactatc tctaatcgtg atttatcaaa ggttgatata ggtgagtttg
     8641 aaaaagtaca agaccttata aaaaaattct atcccaatat attcaaaata tgtgaatttg
     8701 aaaaagttct tcagactggt cttttattca aatggccagg tgaaaaatct gatgaaccag
     8761 tagtacttat ggcacattat gatgtagttc cagcaaatga acaacagtgg gaaaaacctg
     8821 cttttgatgg aattatagaa aataatgaat tgtggggacg aggaacgctt gataccaaat
     8881 gcacttttgt tgcaatactt gaagcctccg aacagcttat taaaactgga tttaaaccga
     8941 aaaatgatat atatctttct tttgctggag atgaggaagt tgcaggacca acatgccctg
     9001 ctattgtaga acttttaaaa cagagaaata tacatcctgc aatggttcta gatgaaggtg
     9061 gagctgtagt agaaaatgta tttcctggag ttagtggaaa atgcgcattg gtcggtattg
     9121 cagaaaaagg tattaccgat atacgtatga gcatgaaaag cagtggaggt catgcgtctg
     9181 cacctcctcc acatacaata ataggacagt tgtcacaggc tgttgttgac atcgagaacc
     9241 atccattcag tttccagctt actcctcctg tgtcacagat gtttaatatt ctgggcagac
     9301 actcgtcctt tgtttttaaa atcatatttg ccaatctgtg gtgcttcaaa ccaattctta
     9361 atctaatgtg taaaaagaca ggtggagaac ttaatgctct aatgaggaca acagtagcat
     9421 ttaccatggc acaaggaagc caggctgcta atgtacttcc tccaagtgct gaatttacag
     9481 cgaacttacg gcttttagga aaagacacat atgaatctgc tgttttacat atgaaaaaag
     9541 tcacagacaa tccagatatt ctatttgaag taatacatgg ttcaaatgga actacatact
     9601 caagtaccga ctgtatagca tgggacacta ttaaaaatgc tatttcgcag acatggcctg
     9661 atgcccttgt gtccccttat cttatgtttg cagcttctga ttccagacat ttttgtaaaa
     9721 ttactgataa agtttacaaa ttttctggga tgcatcttac taaagaacaa agaggatata
     9781 ttcatggtaa taatgaaaga atatctttgg atgcgatata tgaccttgtc cgattctatt
     9841 tacggcttat ttcattagtg tgaactacat cggcaattga attaccgagg attcccactt
     9901 taatatcgta aaagactgga tatatattta aattataatt tacatccaca tctattgatg
     9961 gattattctt atttattaag atataatagt gtgtgaagta ttatttaaca ataaggatgg
    10021 attatgaagg tatataacac actcacaaga acaaaagaag aattagtccc gctcgatgga
    10081 aaaaccataa gaatgtattc ctgcggacct acagtgtaca gctatgccca tataggcaat
    10141 atgaaagcat atatttttat ggattttctt aagcgttctc ttaattatct aggatataaa
    10201 gtaaaaagtg taatgaatgt cactgatgtc ggtcatcttg tgtctgatgg tgatgaaggt
    10261 gaagataaaa tgatgaaaac agcaaaaaca gcaaataaaa ccccttggga aatcgcatct
    10321 atttactcag atgcattttt taaagacatt tcaaaattga acatacaaaa acccaatgta
    10381 gtatgtaacg cttcagaaca tataaatgag atgctggata tggtatataa actttatgat
    10441 gacggatatg catatgaaac aagcgatggg atatattttg atataatgaa atttcctgat
    10501 tatggcaaac tttccagaat ggaccttgaa ggccagatgg ctggtgcaag agtagcagta
    10561 aatgaagaaa agaaacaccc tgctgacttt gctatatgga agaaagcacc aaaagaacat
    10621 atcatgcagt ggaatagcag atggggtatg ggatatcccg gatggcatat agaatgctcc
    10681 gctatgtcaa gaaaatattt aggagatcag tttgatattc ataccggagg tgtagaccac
    10741 attccaattc atcatgagaa tgaaattgcc cagtctgatt gttatataaa aaaacaggca
    10801 gttaaatact ggatgcactg cgaatttctg ctggttgaca atggaaaaat gtccaaaagt
    10861 ataggcacag catatctcgt gtcagacttg gaagataaag gttacgaacc tttggctttt
    10921 agatatatgt gtcttaatgc tcattacaga tcgaaactga actttacatg ggatggtatt
    10981 gaagctgcac agaaaagcct tgtacgttta tctgatctga cgcttgctca taagcaatca
    11041 aaagaggatg taccagatga actaatcagt aatctaaaaa aagaatttac tgatgcttta
    11101 tcagacgatt taaacattcc gttggcaatg ggtacagttt ggaatgcagc acgcaacagt
    11161 acaaaatcac ataaaatata tgaattgtta atggattttg ataaagttat tgcacttaac
    11221 ctggaccaaa agagagaagc tgaaccagaa cagcttcctg atgatataca agaactggta
    11281 aagataagag aacaagcaag aaaaatgaaa gactggaaga aatctgacga gttgagagat
    11341 cagctaaaca gtgcaggata tcttgtaaat gatacaacat ctggtcagat tataaagaag
    11401 gcatgatatg ttagttaata tatttcaaag aggattcaat ttttcacagg acggaccagg
    11461 aaacaggctt gtatatcact tacaagggtg taatatgcat tgcccatggt gttcaaatcc
    11521 tgaaggtata agtgaaaaag gcagcattat aatatcacaa ggaatatccg ataaggtctg
    11581 tcctatgaat gcaattacag atgggttgtt tgacagtaaa atatgtaaag gatgccaacg
    11641 aatgtgtctt gaccattctc atttaggtat caaacttagt tgcgagactt ttaatacaga
    11701 tgaaatcatc gatgaagtaa taagaagcaa atctatgttt ttcaataatg gcggtgtaac
    11761 ttttacagga ggagaaccca ctcttaatct acctgtgttg actgatttat taaaaaaact
    11821 taaagaaata aatataaaca ctgccattga gacaaatggc tctcatccgg aattagcctc
    11881 aatactccct tatgttgacc atatgataat tgacttcaaa cattatgacg atgataaaca
    11941 taggaaaaca gtgggaatat ccaattcaat aattaaaaga aatttgaaaa agttatcatt
    12001 tttagctaaa attaatgtaa ggatacctct tgtaaacgga tttaatgcac aagaggaaaa
    12061 tgcacatgga tttgcagaag cattagctga atgcaacaaa gcaaatataa cagttgagtt
    12121 cttaaaattt catgaatacg gaaaagcaaa gtggaagcaa tgcggtatgg aatataagat
    12181 gacaggaaat gaatacgtaa gccaggaaac agttgatatg tttatacgaa tatttaatga
    12241 aaaagacttt actactataa ctacatagag cactggaaag gagtgtatga ttatgttaag
    12301 actaatcatg caatgttcta aatgaaaaca tatgatattt acacatccga aaaaattaat
    12361 aaaaaagcag cattactttt caaagaggaa agatcactta atcgtcttga cgggtggttt
    12421 attgcccgtg aaatagtatg taaccataac tcagaactga atggtttgcc aaaagaaatt
    12481 cgagcagcaa atgaattaaa gtacataatt agtgaaatcc ccttgtctat tagtgagcat
    12541 aatatctttg caggaacaca acgcgatgca tttgcacgtt cttatgcatt gattaatccg
    12601 gcatttgaag tggaaacatt ttcaggatac tgtgacccta cagctatata tgatgatata
    12661 gagccaaacg agcagtttac agctgaaaga atagaaagag ttaaaaagtt cacaaaaaat
    12721 actgaattcg taaaacagct tacaaaagta tatgatgaat atgaacagta cactaaagaa
    12781 gtagtttttt tcatagaaca ggtcacaggg catgtaatac ccgattttcg ccctgctctt
    12841 aaatatggta ttaaagcaat aattgaagaa acagaaaaaa ggatatcttc tgaagcagat
    12901 gaatataaaa agaataatct taaagcaatg atattgactc ttgaaagtgc agttttgtta
    12961 gctaacagat atgcagatat tgctgtactg cagatgaaaa ctgcatcaga agaaagaaaa
    13021 aaggaactta atttacttga atcaacttta agaaaagtac cttatatgcc tgcaaagaat
    13081 ctttatgaag ctattcagtc ttatcttctt ttatggcagg ttatgtgtat tgaacaagca
    13141 cctaatcctt ttgcattctc tgtaggaaat gccgacagga tttttgaacc atatagaaac
    13201 gatttatcca gagagcaggc agccagtctt tttaaacatt ttcttgtttt tttcaatgtt
    13261 ggcgacaggt catgggccat atcgcaaaat gttcttctag gcggagaatc aaataatggc
    13321 aaagatcaaa ccaatctttg cacatatgct tttttagatg catattatgc catgaacttt
    13381 cctcagccca tcttatcagt aaaattacat aataatactc caagacaatt atatgaagaa
    13441 cttggaagat tcttttttac tccagggtgt cttactcctt ctttatttaa tgatgagtct
    13501 gttttcaaag tactttcaaa agctggagtt gatgagaatg atttagagga ttattcagta
    13561 gcaggatgtc aggaaccttt aataatgggt aaagacaacg gaaatacaac taacagctgg
    13621 ctgaatctgg gaaaaatact ggaaatgacg ctaaacaatg gcaaatcact aatatctggt
    13681 gaacaaattg gtaggaaaag aactgttacc gcttcagatc tgttaaaaaa tataaaagag
    13741 tatttttatg atgatgcaaa atattatata aaacttatga cagatgctgc taatagagca
    13801 tccatagctt tatcagaaat gcctgttccc ttcttaagta gttttatggg tggtttggaa
    13861 tcaggatatg atatgcgtga ttgtaaatat caaggcacga aatataatgc aagcggatgt
    13921 ttaatacatg gattatctgt tattgctgat tcatttgttg caattgatga atatctgaag
    13981 aagtatcctg atgatccaga cagattaatt agtgctatta gaagtaattt tgaaaatgat
    14041 caggatctta gagcattttt aaagtcaaca cctaaatatg gaaataacct tactgttcca
    14101 gacaatcttg ccatggaaat atctaaagaa atatctgatt atgtcaaatc tatgcctaac
    14161 tatcttggta ataatttccg cgctgactgg tctactccat caactcatct tctttatggt
    14221 tactgggtag gagcaactcc ggatggacgt ttatcacgtg aaatgctagg ttatggcatt
    14281 gaccctttat atggtgaggc aacaacaggt ttaggattta gaattctgtc atgcatgaat
    14341 cttccatttg atgagatgaa tggaggatat gcctcgcatt ttggcattga ccctaaattc
    14401 tttactgcaa agtcagatga agaaaaagga cttcaattca aagaaagaat tatacaacct
    14461 ctattcttta atgagcagaa tcagaaactt tgtccatatt atttgtatgt aaatgtcaca
    14521 acggctgaaa cattgagaaa agtattagaa gacccaaaga catatgctcc aagtggtgtg
    14581 tacattatga gaatacatgg tacatttgtc aactttctag acttatcccc tgctatacaa
    14641 aatgatataa taaccagatt ggatcctgat tctacttcaa tataggagtt tattatgctt
    14701 gctttagggc ttgatatcgg aactagtaaa atatgtgtaa ctattattga ctgcaaaagc
    14761 ggtttatgta ttgagaattt tcatgatgat aatgatactt ttattaaaat taatggaaaa
    14821 agttcaaaaa tgcaggatgc agttgcaata aaacagaagg cattaactct tctgaacact
    14881 gcaattgata aatatcccga tataaaatgc atcggagtaa caggacagat gcatggtatt
    14941 atatatttag attcagataa taatatagta agccctcttt atacatggca ggatgagtca
    15001 gcagattcaa attttcatga cctgaatata tcatatgcta aaaaattatc cgatattact
    15061 gggtataaaa tggcctcagg ttatggacta gcaacacatt tttatcattc agataataag
    15121 atgataccca aaactgaagt taagatatct accattcacg gatacatagc ctgttctctt
    15181 gctgaaaatg aaaatatcat cattcattct tctgatgcac attcattagg aatattcaat
    15241 atcaagaaca gcagattcga taaagatgca atagaaaagg cacaaattaa ttactccatt
    15301 ctgccaaaaa caattaataa accatatatt ttaggtaagt atctcgacaa aataccggta
    15361 tgtgttgcca ttggagacaa ccaggctgga tttataggat ctggatgtac aaatgatatt
    15421 ttagtaaata ttggaacagg aagccagata tctttaagta caaataagtc tgaaactaaa
    15481 agttcatctg ttgaaatcag acctttgaat tatgaccagc acatttttgt aggagcttca
    15541 ctctgcggag gaagagcttt tgctatttta aaagattttt atacagatgt tataaaaatg
    15601 gccaatcttc ctgttccgaa aaatctttat gatcttatgc ttaataatct ggaaaatact
    15661 gatttaactg atagtattgt atttaacacc ctgttctgcg gaacaagaga tgactcctca
    15721 ctgaatgcag gtatttcgaa tatctcaaca gataatttta atgccgggaa tatgactaat
    15781 ggtacattag aaggcattgc agttgaactg tataatatgt ataagattat aaagaaagac
    15841 gatgataacc caaaaaacag actaatcggt tctggaaacg caattagaaa gactcctcct
    15901 ttacgaagga tacttgaaaa gaaattcaac atgccgttgt atatgcctaa gaacagcgaa
    15961 gaagctgctt ttggagctgc tttatatgct tgtgtagcaa ttgggattta taataattta
    16021 gaagatgctc agcaaataat tcaattcgag gaaatagtat gaaaaataat ttaaacatgc
    16081 tgttaaatag accaattttt tttgaacgaa acagagtttt cagggtatat aaaggcggat
    16141 tgcttttcaa taaccttttt aatgataagc ctgaagatgg aaattatcct gaggaatgga
    16201 tagcttcgac cgtcaaagct ctcaataaga taagcagcgg acctaaagaa ggtatatcta
    16261 aaattgaaga ttcttatatt tatcttgatg acctaatagc tgagcatgaa cagcagatac
    16321 tcggttccca aaaagacata ggaatacttg taaaagctct tgatagcgct gtaaggttac
    16381 cagtccaggt ccatcctgat aaagatttct caaaaaaata tttcaatagt gaatatggta
    16441 aagctgaagc ctggattgtt cttgataccc gtcccggatc atgtatttac tttggatttg
    16501 aagacaatgt aacaaaacag gacttataca ctgcagtaaa aaacagcgaa agcgataaat
    16561 cagcaatgga aaaactgata atcaaacatg aagttaaaaa gggtgatgtt tattttatcc
    16621 ctgcaaaagc tgttcatgca attggttacg gatgcttcat tttagaaata caagaaccta
    16681 cagattttac aatacagcct gaagcttggt gtgatgatta taaactaaat gactatgaga
    16741 tgtatatggg gcttgatatg gatactgctc ttagctgttt cgacatgaac atttctggta
    16801 aagaagcgat taatatttca aaagttaaac ctgtaaacat atcggaaaag aaaaatgtca
    16861 ggatagaatc cttaattgat agaagaaaca ccgattgctt cagagttaac aggataaata
    16921 ttaatcgtga tagtttcctg cttaaacatg ccccttcaat atatgttgta atagaaggca
    16981 atggaattat atcttgtgaa cacagctcaa gacttgttaa aaagggagac tattttctac
    17041 ttccctattg tttaaaaagc aaatgcatac tttcttctaa cgaaaaaatt acagttattg
    17101 aatcttttaa aggagaaaca gattgattga tttttctgat tatcctttga gcataatcag
    17161 cagtataagt tatggaggtc atgtccaggg tatagctgtt gataagaaaa atagttttat
    17221 ctatctttcc tttactacag agctgataaa agctgattta tctggaaata ttattggatc
    17281 aattaaggga tggatcggac atttaggctg tattgattat aatgatgatg atgagtgcgt
    17341 atatggttca ttagaaatta agaatgattc aataggaaaa tcaataaaag aacttcttaa
    17401 gactgataaa acaataccag attgcttcta tattgcagtt tttgacacaa aaagaataga
    17461 cagaaaagat attaatgcca aagattcaga tatattaaag acttgcttta taaaagaatg
    17521 tacagatgat tatataaaca aaagatatgg atgttctgga atagatggaa catgcatagc
    17581 tgatgatata gaagataatc atgacaggct taactatctt tatgtcgcat atggtattta
    17641 caagaataat caggaaggct taaacgatta ccagataatt ttaaagtata caattaaaga
    17701 tattttgtca aactgtattt ctttcaatga aaatcctttt cattcaactg gtccctcaaa
    17761 acctgataat aagtatttta tatatacagg taatacaaat ttcggagtac aaaatctaga
    17821 gtacgatgga tatactaaag actggttaat ggctgtatat aaaggagaaa aaccgaattt
    17881 tccgaattac tcgtactata ttattgacgg aaaaactaaa ccagaaatta agaagataca
    17941 acaatattca gatgaactgt attacaacct gttatcttta aaaaaattgc cctactctga
    18001 ctcacttact ccaggcttta attttgaaag agggcaagag ggtatatatt catttgacaa
    18061 tggttatttt tacattgcta aatcaaaaag gtcagaagat ttgggatggt atgcacagat
    18121 agatatgtat aagatatcat atgattctaa gaatattttt gaaaaggtag tatatggata
    18181 acaactctaa acatacatct aaaagtatta aaagcagtct catgttgcta ttaacttcat
    18241 tcatatgggg atttgctttc gtttctcaga gaatgggatt agctacgatg ggaatgcata
    18301 ccttcaatgc aacacgtttt ttattagctg ctttcgttct gttgcttttc atactcttta
    18361 cggatattaa agataaaaaa aaagcgattg ttatcggtta cacaaaaaac aataaaaagc
    18421 aaagaagaaa tctgataact gcagggacaa tatgcggagt tattctggca attgcaaaca
    18481 ctctgcagat ggcagggctt caattcacta caataggaaa agcagggttt attacagtat
    18541 tatatataat tattgtccca cttacaggta tcttttttaa gaaacatatc aatttatctg
    18601 tctgggtaag tgtaatgctt gcattaggcg gttcatactt attattcata caagaaggat
    18661 tcagtattaa caaaggggat cttcttatat taggatgcgc atttcttttc gctgctcata
    18721 taatggttat tgaccactat acaaaaataa ccgatagtgt aaggatggct ttcatacaat
    18781 tctttgtatg tggtgttatt tcaatgatag ctatgtttat ttttgaacaa ccagacataa
    18841 aagtcatact ttcagcatgg ctgccaatag cttatgcagg cgtactttca tcaggagtcg
    18901 cttacacatt tcagttcata gcacaaaaaa acttatctgc acctgtagcc tcactgataa
    18961 tgagcatgga atcagtattt gctgtattgg caggatggat aatatttcag gaaagtctat
    19021 ctgttaaaga aatcttgggg tgtgtattaa tattttttgg gattctaata gttcagattc
    19081 catttaaaaa caaaaaaatc agcaaacaga tttaaactta ttttttcatt aatctttatg
    19141 atacaattag tataaattaa agaaaggcag agctatgaat attcaggaat cagctgaaat
    19201 gtatcttgag actattctca ttctcagtaa acaagcagat cagatacgtt caatagatat
    19261 agtaaactac actggttata caaaacccag tataagcaga gctgtaaatt tattaaagaa
    19321 gaacggttat cttgaaatgg atcagaaagg cttcataaca ctgacaaaaa acggtgagga
    19381 aattgcaaag actatatatg aaagacactg tgtacttacc gatcttctta ttaaaatagg
    19441 tgttgatgaa aaaacagcca gacacgatgc ttgcaaaatt gaacatgtta ttagcaatga
    19501 tacatttgaa aaattaaaag aatacagata atgatgcaga agggagagta tgattaattc
    19561 agacactaat catacaaaca attatgatac caagagatag agctgttaca gctctaagcg
    19621 gaggtatacc tgatgaaata cctacatttg aactggaatt ccagctttca atggaattac
    19681 tgggcaagga attcttaaaa caggaagaac tcaataaaat gagctctaag gaaaaagaca
    19741 tcgcacttca tgagaatgct gaatttatga ttgaagttta tgagaaactt gaatattcca
    19801 taatccctat acactattta tggattgatg atgcaatcaa aacagcaaag attataaata
    19861 gtatgaccaa tttcaaatat ctgcttacaa gacatggtga tggtacatac tccatacctg
    19921 acggaaatga aatgtatgag ttcgcatatc gtactataga tgatcccaaa ggccttcttg
    19981 aacaagcaga agcaatggct gtaaacgcaa tagaaagaaa caaaaaagca atggctggtg
    20041 gtattgaaag ctttatactc tgttgcgatt actgctataa caatggtcct tttttatccc
    20101 ctgaacagtt tagaatattt ataacaccct ttttagctaa gattataaaa ggaataagag
    20161 acgaaggcgg ttatgcaatc aaacacacag atgggaatat aatgcctata cttgaccagc
    20221 ttattgaagc taatccgcat gctatacatt cacttgaccc aatggctgga gtagatattg
    20281 caaaagtaaa ggcaatcgca ggaaataaag tatgcctaat aggaaatgta aattgtgcat
    20341 tattacaaac aggtacagac ctagaagtta ttgacagtgc tgaatactgc ttgaaatatg
    20401 gcaaacccgg aggtggatac atatactcaa caagcaacgt cccctttaaa ggcttaaatc
    20461 ttgacagata tttattagtg ttggatgtct ggaaaaaaca tagaaagtat tagatataga
    20521 taattcccga aataccggcc cttttttttg acgcctagcg ttagccaggt gggttctgag
    20581 atgaatgttt ctgttttccg tcaagtacgg cctgacatct gcattcatac gtctaaatcc
    20641 cgtcaaatta ctgtaggttc aaatacaagg gttatcgaat acctcaggaa ttcttctata
    20701 tacattatac tatcaaaagg aacttatatc aatataatat attaattaga ctttcactaa
    20761 acactataca ttaactaatt cttcttattt aatattgttg cttctagcat aataaaagag
    20821 atattgctga gcatatccag ctaatggtcc aaatttatcc tgaccatatt tttgaacata
    20881 atctaaggat acttcctttt ttatatataa attttccatt attcttttta cccaaacatc
    20941 agttggaaaa gcatatgatg aaataccgga aaataatatt atgcaatcag caaccttact
    21001 gcccactcct ttaaagttca taagttcttt ttttgtttgc tcatacccta ttgatttata
    21061 tctgttcaca ttaaaatctg taactatctt gtttatcatt cttgaggatt ccaatatata
    21121 attacatcga aatcctgcac gacattcatt aatggtacat aaatcagatg aacttagttt
    21181 tttaacagta gggaatgcat atctttcttc acccaatttt cgacccagaa taatacttaa
    21241 gtctcttatg ttattggata ttcttttaat attactattt gatgatgtta tgaagcttat
    21301 agtggtctca aacagttcct gctttaaaat ccttatgcct ggtgagcatg taattgcttt
    21361 atacataata ttatcttttc gcaattcttc ttttataaga tcataatctg tatcaatatc
    21421 gaaatactct ctccaaatat tattatagtc ttctttgttg caattaagtt ttatgaatcc
    21481 atcatgttgt tcagctttaa ggatcttttc ataagcaata ccaatgaaca atttttcatc
    21541 attcagctgc tcccatcgaa aacactgacc acacttaaaa gtatctgaaa gacttaaata
    21601 atcctcattt gcattttcca atatcgtata gtcagtaaaa aatgattctt taattttcat
    21661 actctatgct tagcatgcag tgtccttcaa tcttgtccac tataaaagaa attttgttat
    21721 ttttctggtt aaattttaca ggggttgact gcggaacaag actgatattt tttatctgtt
    21781 tatctagttt taacgttatt ttagttttat ataaaggaat cacatcttct attgtatcaa
    21841 aatctgtact ccttctttct ggtatatagt gcagcatatg tagtatgtac ctattttcct
    21901 gtggctgctc attcattgcc ataaacaagg tcgaaggacc attatgtgaa accattttat
    21961 cttttaatag gatttctaga gcatcggaaa gaatagattt gatccatttt ggtccttttt
    22021 tgttgtaaat ctcaaatata ggatgactga aatatatagc tttcttcttt ctgtttaata
    22081 taacagcatc atatgcctct ttatgagaac tcggactttg caagtgcgag gaaaaatgtt
    22141 tatatgacct gttaaataca ctttcatatg cttttacaag actatatcct ttatctgcga
    22201 ttgtctcaaa accttttgca tacattacat attcagtttt atataaatcc ttgcctatct
    22261 tgtcatttgg cataatataa tctgaatatc cgtttcttgt tataaatctt cctttcacgg
    22321 gctgtttata atcatcaaca gtttcattta taaccttgac agacagccct ttaaatattg
    22381 aatcatagac tgctattacc ttaccgccat tttccatata ctcatttaat ttgatttcca
    22441 gctctgaatc tataccaatc ctctcaggaa taatgattaa tctgtattta taaaaatcca
    22501 tctgcttgtc aataaaatcg aattggtatg aaagctggtc cagtatgctt tctatagcag
    22561 cctgagaggc cgaaactctg cttttctcat ctgtaggtac cagtacggct aactcagcaa
    22621 tatgctgagc atttttgcac catggctctt tatcttcaac tttggaataa acttttccaa
    22681 taaggtcata taccggttct gataaaatcc cgcttggctc taattggtca ccaataaggc
    22741 acttcgaatt cagagcaagc attctaaata tttcatattc caatgcttct tgatttttaa
    22801 aagaatgaaa atccccccag ctggtatgaa acttgcctgt atgagcaaga taatcttttc
    22861 ctagatttct tgtatatctg caagtaactg gaaaatgcat ataaccccat cccccacttg
    22921 gcagactctc tatctcatag tgcgaatatg tatcttttga aggttctttg tctttatacg
    22981 atacatgtcc gctattaaag aaaacaagaa tacttggttt caatgaccac actaaagatg
    23041 acatttcttt tttaaattcc cgcataacac ttttgtaatg tgttatcctg tcatcttcaa
    23101 caagcgggtc caagcctttt tctaacatac tgttcataca gtacgtacaa gaacagtcca
    23161 tttcactgac aatatcaaaa aatactccat cacaatccag ttcagataca aactccctga
    23221 cgtgtttttt taagaaatcc ttatatggtg tattaacaca tattctctga taaaacccat
    23281 ccttattgaa atactcacct tctatagaac catctggttt tctgacaagc cattcaggat
    23341 ggcgtattgc agtatacata tcccattgga cagttgtgta tacaggaact tttatgtcat
    23401 acttatggca tgcatcaatt tgctttttca ataagtcttt gtcacaaaga tagggatgaa
    23461 ttctctcttt gttaactttg gagtcataat aaatcatacc atgatggcat cttgcaaaac
    23521 atgtaatgga atttacatgc gcatctttta gagtcttggc gaatttctct ggttcaaacc
    23581 ctaaagctat atctttaata agttcacttg tgtgaaaatc caaatgtatc tgtctgaaac
    23641 gtaatctatc ttccataatt ttttcctatt ctcctacatc acatttattt atatttaagc
    23701 aatccatgcc taaaattgca ttgcacatag gcatgaatct ttctatactg tctgatgtgt
    23761 aaatttctaa tatacctttt gatgaagact ttttgagcaa tcccgatttt tctagcataa
    23821 tttttgtttg ctttgcagta atatatgctg aatctacaag acaaacatca tttcctatag
    23881 tctgtcttat tgaattttta agtaaaggat aatgagtaca tccaagtata agtgtatcaa
    23941 tgtcggtgtt atcaaaaaaa ctgaaatatc tcttggcgac cattttagtt atttcatctt
    24001 cccaccattc aactccttct tcggctagag ggacaaataa aggacagctt ttctgatgta
    24061 ctgtaacttt tgtatccatg gacttaagag ctttttcata agagtgactt tctatcgtag
    24121 cttttgtccc aataacacca atgttcttat tttctgactt attatatgca agttcagctg
    24181 tagtagatat aacttctatt atgggaagat catagtgctg ttttattgca tccattgcat
    24241 tcgaagagac agtattacat gcaatcacta tagcttttgc acctctttga gtgagaaatt
    24301 tcatactttg taaagcaaac aactctattg tctgtttaga ttttccacca tatggagttc
    24361 ttttcccatc tccaaaataa atgatatcct cattgggtaa ctgtttaatg atttccttta
    24421 aaacagtaag tccgccgatt cctgaatcaa aaacacctat cttgccgtta atcatatctt
    24481 attccttcat aatcagtttc aaatatatat ttttcttttc tttcttcagc aagtttaata
    24541 agcttatcaa gcagttttgt atatgatatt ccgctttgat cccataattt gggatacata
    24601 cttatatcgg taaaacctgg tattgtattt atctcattaa caattgcctt tttccctttt
    24661 gttaagaaga agtctattct tgccatacca tagcagccaa cacacctgaa tgcctgctcc
    24721 gctatcttct ttatctgtct tgttgaagaa tctggtatgt caggtttcaa caatgtactg
    24781 cttttaccat caatgtattt tgcattatag tcataaaatt cattagatga taatatctgc
    24841 ccaactcctg aagaagatat cttctcgtaa gaacccaaaa cagctacttc cagttcatca
    24901 ccttcgataa actcttcaac aagaactttt ctggaaaaca tagcagcaaa aaccaatgct
    24961 ttctttaaac tgtcagcatt ttttgctttg cttataccaa cagaagaacc gcattctgac
    25021 ggctttatga ataccgggta ggcaaattta ctctcaattg atttaaccac tttatttatg
    25081 gattttttta tttcttcaca tgaaaaccat aaataatctg caacagctat accattttta
    25141 acaagaatat ctttgcataa aaccttgtcc atacatatag ctgatgataa tacatctgat
    25201 ccaacatatg ctacattgca taaatccaaa tatccttgca aagtaccatc ttctcctttt
    25261 gttccgtgta aaacaggaaa gacaacatct atattcatct tctgaaacat agctatatca
    25321 ggtacaatct tttctttgtc taatttgttg tttatctgaa ctaactgctg gtaatattca
    25381 acattatcat gatattcata taggtaatac tgtctatcct ttgttatctc aataacataa
    25441 ggatgatact tctttttatc aagatttctt aatacattta cagcagatat ccttgacact
    25501 tcgtgttcac aggatatacc tccgaatatt atacagatgt ttttcatatt atttttcctc
    25561 ttttgtataa tagcagtctt ttatctctcc aaaatagaac atatggtaat catcagttcc
    25621 ataaaacctt tcttttattg aagattctaa agctgttttg tccattgctg tcttttgaac
    25681 tacttcgcat tctatatgaa gatcacattt atcgattatt ttatcttttt tgaaactgca
    25741 tgtttcaaac ttgtctatat ccctacctga ttttgttcca catattccaa gttctttctt
    25801 taaagtacct gttttgggta tgcttatcgt aaatttatta gatttttcca aaagagagta
    25861 agtgtgcctg gaaaatctta cagcaacaca tactacaggt ttactccatg taatcccaac
    25921 tagtccccat ccaatagtca tagtattata ttcaccctca aaactggtgt ttagaaaaac
    25981 accttttgga agctgttcat taaatctttt aaagatatct tcataattta catgttcttt
    26041 catatcatcc tcactagttt ttattaatta tatcatgcaa acaaatatat tcatattgaa
    26101 tatgctaatc tatttcagag tgaaatcctt caagtattgc ttcccataca gttttatttg
    26161 tctgactcat catccatatg catacgcctc ttaagtcata ctttgaagca agctgagctt
    26221 ttaaccgagc actggcatca tcttcaatcc aagctctgta taactcacca ttttcccagt
    26281 attctatata gtactgtcca ctgataaatc catctctttc ttcttcccaa atcggtgaaa
    26341 gctgttttga ctctactatc tcaacaacct ggtcagctgt aagagtccta ctaagagtca
    26401 ctttaccatc attatctgtt ttccaaagcc ttgtatatgc tggaatccct aaaaccagct
    26461 tatctgatgg tattagcttc aaggttgcaa gcaaatttgt ttcaacccat gacagttgag
    26521 cctgactacc tccgctttca tgtgaactcc agtactggtc atatgtcata accattacaa
    26581 aatcagccgc ttttccaagt tcataatgat cataacataa ggaatatcct tccgatccgc
    26641 ctgggatacc cacatcaatc gatacggcta aattcatttc atgggcaagc ggtactagct
    26701 ctcgtatgaa ctgagtcaaa ttatctttgt ctgaaagata gagattttca aaatcaacat
    26761 ttattccatc aaatttatat atatatgcat atgtaagtat ctgttcaatt acatattttc
    26821 gggcttctga atcagccaag acagcactta tctgatctct gttagaaaac acattattaa
    26881 ttaatggcca cactgtatat cctcttttat gagcattttc catatatttt aatgatgcgc
    26941 tgcttgctat tgaccccttt tcatcaatga cagtaaaaaa tgtaggagaa atgacattta
    27001 catcagcata atatggtacg cttgtaacca gacttgaatg tacatagtgc catgtaagaa
    27061 caacatggtc agggatgaat aatttttttg gttcatactt aataactacc tgcttagatt
    27121 gaaagtctgt atttaaaaat tttgtttcaa taaatcccac tactccatta gcagtcatta
    27181 caattgtcca ttctccttga acttctttta cataaagcat ctgatcttga ggaaaatcct
    27241 ttatgtatat aggttcttta attgatggtc ccttcctaat aacagcatca ggtatattga
    27301 caacagctgt ataatcataa cttttgtatg attcaattat agctgtatca tattgagaaa
    27361 tatactttgc attaatattc cataattcca taaaagcact tatgggtaca tacagatatc
    27421 catcaaatcg ctggcttgct gtatcaagat tcatctcatt tttatttatc tgtgctataa
    27481 ggctgttttc agtaagacgg aaaactttat ctgatgtagt tattattatt ttcccgcgat
    27541 cttcatcata atatatataa gggtcaatgt attttttaat aacttcatat ctgattaata
    27601 tttcattatt tattatcctt accggtttat caagttcaat acgtgaaaaa tcgacaatca
    27661 aatcagtttg gtcatcagaa aaatactgtt caatattttc atttggggaa tatttatctt
    27721 taatgtattc aaatataaca ctatacgcat aaagcgctgc tgacaccaaa atcagtatta
    27781 ttagcgttaa tataaaatat ttccaaagat ttctcaacta ttactccttg aacttatact
    27841 gcacgtactt tttaataaac tcaagtaccc tctcatctga ttcaaagtaa acaagagttc
    27901 tttgagattt atagtatgct acaccaaaaa gcgtatcatc tgttgaagat gacgcatcta
    27961 ttacctgttg aacattatcc ttatactcat ttatatgtac agaattttta tatgctataa
    28021 cctcaaaatc ctttaaagaa agggtaaaaa tgtttttacg tctgctttta tttattattt
    28081 tatcaatagt catggtatct tcaacaaaaa tatactcata atcaaatttt gtagaagcaa
    28141 ctagtctgta gcatgcatat attactccga gccatattat aaatgaatac gtcttgagaa
    28201 aatagtagga tataatacat atagccaggg tagcaacaac agcaccaagt gatataaggt
    28261 catctttccc agttcttttt ctgtatacta tcttttcgta ataccagctt tccatgtgtc
    28321 acatcctttc aatataataa atataccata cataatcaaa ttatgtatgt attttttatg
    28381 aacattagat ttaacatatc tttattgtac tatttaaaaa tcatcttgta atacttattt
    28441 atagcaaatg tgataaaata aaatacaagc cggaggtacc ttatgaaatg tgcaatagga
    28501 caagacagcc acagattttc gaaaaatagt caaaaaccat taattctggc aggagtcctg
    28561 attaaaaacc ataaagctct tgatgcaaaa agcgatgggg atgttattta tcatgcactt
    28621 acaaatgcta taagcggtat tacatctgtt aatgttctgg gaaaaactgc tgatgaaatg
    28681 tgtaaaaatg gaattattga tagcaaattt tatgttctgg aagcacttaa aagcctacaa
    28741 catgatatct tacatgtaag ttttacagtt gaatgcaaaa ctccagttat ttcttcatac
    28801 atagacaata tgaaagaaaa catttcaaat cttctaaata tcgataaaaa agatatagga
    28861 ttaactgcta catccggtga aggtctgaca tcattcggac aaggtgatgg aataatgtgt
    28921 ttctgcttaa tcaccgttgg ataaaaaggc ctctgctatt agcagatcaa tttttgacgt
    28981 aatttttata ttaagcgtat ctgactctat gatctttaca ttgtgcccgt aatattcagc
    29041 aatcatgctg tcatctgtaa cattaagatt ttcttcttgc gcccttttat gaaaatcaag
    29101 tgcaaattcc gttttaaatg cctgaggagt ctgagcgtta taaattctct gtctatcaag
    29161 tgtattttca acaaacataa tattatcggc tcttttaata gtatctgtgt ttcttgaagc
    29221 agctaagcag aacccatatc ttttagctcc ctcaatacaa tcatcaataa tttttttggt
    29281 aactaatggt cttgccgcat cgtgaataag tacaattcct tcatcatcac ttagctcttt
    29341 taatccatta aatactgatt cacgtctgct aagtccagaa taagcaaatt taactttact
    29401 gtctacttta tattctttta taagcttaag atataattca tgctgctctt tgggtaccac
    29461 aacaactatc tgctcaatgt attccagttc atcaaatgct tttatggtac gcactataac
    29521 aggtaatcct gccactgtag caacaagctt gtttatcccg ttcatcctta tagatgagcc
    29581 gcctgccgga atcaccgctc ttataggttc cattttctaa tcttggcctt cttgttcaga
    29641 ttcacagttg atgctttcca ttaaagcttc ctctgcttgt gttaatgtga tattttttgc
    29701 taacatgatt tcgcttaaaa cagcattttt agcatttagc attaactttc tctctccagt
    29761 tgaaagactt ttatcgcgcg ttaaaagtga ttttgctatc tgagcaattt catcaatggt
    29821 accatctctt aatttttcca tattctcgcg atatctttta ttccagtttg ataattcaac
    29881 tgtattcatc tcttttattt tacataaaag ctcatcagct tcattagctg acattatatc
    29941 tctaattcca atagtatcca tgttttcaac aggaagcata gccaccattt cactaactgg
    30001 gctcttgaca ataaaatagc atcttatttc atccattatc ttcttattta ctttgtcaac
    30061 gataatgcct gcgccgtgca ttggatatac gattttgtct ccgatactgt aactcaaatt
    30121 aatacctcat attaaattat actctatttt taactaagtg tcaaaagacc aaaatcaaaa
    30181 aacaaggtta cttaaccaaa gcttcttcta ttgcctgcga tatagctgaa acaccgatta
    30241 tttcaatctg attttcatat tgcatagcgg attttaaatt cacgtttgga atgattgctt
    30301 tagtgtaacc catcttgaca gcttcattaa ttcttttatc tatcccatta acagatctta
    30361 tctctccagt taagccaact tctcctaaaa caacacacct gctgtcaagc ttcttgcctt
    30421 tgtaacttga tgcgattgca gttataactg caagatcaga tgcagtttca ctaatcttta
    30481 ttccacctgc cacatttata tatgcatcgt aattatacag tctaatttta gcattttttt
    30541 ctatgactgc cattaacatg ttaactctat actgatcaat tcccgcagac atgcgtctgg
    30601 gcattgtaaa attagtagga cacactaagg cctgtatctc aaccatgaga ggtcttgagc
    30661 cctccaaaac aggcattaca accgttcctg gagcatcaag gtttcttcct tcaagcataa
    30721 atgcagaagg atttgtcact tcaattaatc ctttatcaca catttcgaat acgcctatct
    30781 cattggtaga tccaaaacgg tttttaacac ctcttagtat tctaaatgat gaaccgctct
    30841 caccttcaat atataaaact gtatctacca tatgctcaag tagttttgga cctgcgatgc
    30901 ttccctcttt ggttacatga ccgacaataa acagtgcggt atctgatttc ttggcagcat
    30961 taactaacat aagtgtggaa tttttaatct gattaacact acctgcaata gatgatatct
    31021 gacctgaata aacagtctgt attgaatcaa caattgcaat agcaggtttt ttatcatgaa
    31081 tagctttaca tattacatca gtatctgttt ctgacgctag ttggacattc tttcttttca
    31141 aacctattct ttttgctctt agtcccagtt gacttatact ttcctctcct gatatatata
    31201 atatctgatt atcagtacat acatccctgc ataactgtaa aagaatagta gacttgccaa
    31261 tacccggatc accgcctaca agaactactg atccttcaac aatacctcct ccaagcactc
    31321 tatcaacttc agtcatacct gaattaattc tatgtgaatc tgagtaatca atattatcta
    31381 ctgaatccag ttgtatttct ctgacactat ctgtttttaa attcttaagt tcagtaactt
    31441 cctcaaatgt gccccattcc tggcatgaag ggcatctgcc catccatttt acggtttcat
    31501 aaccgcaaga tgtgcataca aaatagttgc tatttttggc cataagccct ccttatataa
    31561 tcattttcat aagcatgttc tttaatatat ctgcacgtac cttatctctt agaaatactg
    31621 gcacgctttt taaagaatta tcatttacag ccgctttaag taatttttta tcagattctg
    31681 atattatatt taggtccata aaattatcta tatatacttc agcagaatgt ctgcttatct
    31741 catctcccat agataaaata atatgcataa aatagtcata cggcttatcc gttgacattc
    31801 tgctaatagt tatatgaccg cctccacccc tcttgctctc aacaaaataa cctctttcag
    31861 aattaaacct tgtagcaatt acatagttga tttgagaggg aacacatttg aaataattgg
    31921 caagttcatt tctttgtatt tctacttccc cttctgtatc atcaatgaga tcttttataa
    31981 agtcttctat tacgtcactt aatctagcca tcttgaatca ccttttgact ttctctgact
    32041 ttattatatc aaaatcaatc tttttttcaa ccatatattc aaaagcctat ctcgtgatgg
    32101 tatactttat atataaatat tctggagggt aacatggcca aactatattt cagatatgga
    32161 gcaatgggta gttcaaaatc agcaaatgca ataatggttg tgtacaacta caaagagaga
    32221 aatcaatcag cagtactgct taaaccacaa agcgatataa gagatggcaa gcagataaat
    32281 gtttccagat gcgggcttag gatggattgc gaatttactg attcgttttt ggatcaaaac
    32341 gatgacgaat ttaataaata tgactgcata gtagttgacg aagctcagtt tttaactaaa
    32401 aagcaggttg aaacacttgt acatattgct gatgatctgg atataccggt tatttgttat
    32461 ggattacgtg ctgattttca gaataaatta tttgaaggaa gcttatacct tctttcatgg
    32521 gcagatacta ttgaagaaat caaaacaata tgctggtgtg ggaaaaaagc tacatgcaat
    32581 gcaagaattg gagctgatgg taaaataatg cgagaaggtc cgcaattttt gcttggcggt
    32641 aatgaacgat atattccatt atgtagaaag catttcagat ccggtcagat aaaaaaagaa
    32701 gaggattaac ctcttctttt tattgcttaa cttaacatta tcaatattct atatagtatg
    32761 gagttatata atataattcc atttactgta attaaatatt ttctgtgtat gtgtattctc
    32821 ccggacctac attgtatgtt tcttttaagc atatggtcgc agtagtattt ttaggaacaa
    32881 caaatgtata tgtaatgcta tcttttgaca tctcccattt tgaaataatt gtaccctttt
    32941 ttgttatcag acttgctttt gcataattta atccgcatcc ttttacaacg accggtttta
    33001 agataatatt actaaatcct ggtttagatt catctattct tattcctgca actttttcat
    33061 acatgaaatc cacaacagaa ccatatgcat aatgattaaa ggaattcata tccttgctcc
    33121 aaaaagttcc atcaggctta atattatccc aatgctccca gatagtagtg gctccttttg
    33181 ttatgggata catccatgaa ggatattctt tttcaagtaa gagcttatat gcaagatcag
    33241 cataaccata ttcagcaagt acatgcaata tatatggggt ccctaaaaag cctgttgaga
    33301 ttttatcgcc aaagtcatga attcgttcca caagattagc aatggctttt ttctgtcttt
    33361 cttcgtttaa catattgaaa tgcaaagcta aaacatagga tgtctgagta tctgttatta
    33421 gtttttcgtc tttccaataa gtttcattaa atgccatttt aatgttgtca tacaaatcct
    33481 gataatactt tattaccttt tttttcttct ttagtaaaac agctgttttt ttcataatat
    33541 ctacactgtt tgcataatat atagtagcaa caagatattg atttgtttta cctctatatg
    33601 taccaaaagg aacatccaat gccaaccagt caccatattg atatccagta tcccaaagat
    33661 aaggattatc tgaagaattc ttaatgtatt ccacccaaga ggccatagat tcatattgat
    33721 tatctaaaat agatatatca ttatatgtta aatacatttg ataaggcact attgttgcag
    33781 catctcccca ggctgatgat acatttacaa aagagtattt aacaggaatt actgaaggca
    33841 ctgctccatt cattgcctgg cataatgata aatcagtaag ccactttgag aagaattttt
    33901 taacatcata attgtatgat gctgctttgc agaatacctg tgcatctcca gtccacccta
    33961 aacgttcatc tctttgtgga cagtcagtag gaatatcaag aaagtttccc ttttgtcccc
    34021 atatagtatt ttcatataac ttattaagca ttttatcaga actttcaaaa taaccagtac
    34081 gtttcatgtc agaatgcact acaatggctt taaaatcaga tatattgaca ttttcctgcc
    34141 attcatctaa tcttacatac cgaaaacctt ggaatgaaaa atgaggtgag tactcagatt
    34201 taccaccgtt agagatgtat tctatttttt gttttgctcc tcttagattt tcaatataga
    34261 aattaccttc gttatctaaa acttcagcat gagataatat aaatctttca cctttttttg
    34321 cttttacttt aaatctgaca taaccggtaa tattttgtcc aaaatctaaa actctttcat
    34381 tttttggtgt aataatcagt tttactacag ataactgttc atgttctttt acatactcac
    34441 cctgctgcaa aactatatca ttaggattat atccctgtgc aagtcttact ggcttaaaat
    34501 cgtatggtct aagtcttgca tcgtaagtct cgccatcata tatatcgcta aaaataatag
    34561 atgtttttgc agctttccat tcatcattaa cacaactgat aaacgaatct tttgtttcgt
    34621 cagcatagac aacatcaaga gagcacgcta aagcgaaatg cagtttatct gggtcgcctg
    34681 ctatatgtcc tgaaaaccaa ccccttgcaa catatacaga tatatcagca tattcctcta
    34741 taatgttttt tgttacatca aatgactgat attgtactct tctgttataa tgtgtccacc
    34801 caggtaaaaa tctatagtct ttacctactc ttttcccatt gacaaatgcc tcataaagac
    34861 ctaaagctgt aatatttaat acagcttttt ttacttgctt ttcaaacttt attgttcttt
    34921 gaaacattct gcaggaatca ttatttggct caatatcagc cgtaatccat tttgctaaat
    34981 taattgaatt catttctttt ttgtgtgata cagtcagtga ctgttcatac cctccttact
    35041 ttattttaac aggtaaataa ttgttattta tcatgataaa acttttatgt cttagtaata
    35101 ttatcatagc ttgctaagct ggtccacata taaaagagca gttaaactgc ccttttatgt
    35161 atttgataaa ttaataatat tataagatta taattcttat tcctcttctg cttctctctt
    35221 agctgctgca actacctttt cagctagatt tgaaggcatt tcttcgtatc tctccaattt
    35281 cattgtaaaa gaacctcttc cctgcgtcat acttctaaga tcagttgcat atttaaacat
    35341 ttcagcatgt ggaacttcag ctaatacctt ctggtaaccg ttttctgcag gattcatgcc
    35401 aagtattctt cctcttcttt tattcatgtc gccaattatg tcacccatgt atgaatcagg
    35461 aattaatacc tcacaggata cgataggttc caaaagaaca ggagtcgcat caataagttt
    35521 tttatatgct aagcttgctg ctattttaaa tgccatttcc gaagagtcaa catcatgata
    35581 tgaaccatca atcaaagtag ctttaagttt aaccatagga tatccagcta aaacaccttt
    35641 tacaacgcat tctctcaatc ctttttcaac tgcagggata tagtttttag gaactgatcc
    35701 gccaaagatt ttctcttcaa aaactaaatc ctcagtttca cctggttcaa atacgatttt
    35761 aacatgaccg tactgtccgt gcccaccaga ttgcttctta tgttttcctt ctgattctag
    35821 ttgcttttta atagtttccc tgtaaggaat cttaggatca gtaagtttaa ctgatactcc
    35881 gtattttgat ttaagcttac ttgttataac atcaatatgc tgctcaccaa gaccacttac
    35941 aagtaattgc tttgtttcag agttgttttc taccctaaat gtaggatctt catcacgaag
    36001 tttgttaaga ccagatccta ttttatcttc atctcccttt gctaaaggct caacagccaa
    36061 tgatatggaa gcttctggaa atactacttt cttaagttca acagggttta attgtgaaca
    36121 taatgtatca tttgtttgag tatctgaaag ttttgcaaca gctattatat cacctgctat
    36181 cgctttttcg cactgttcct gctttttacc tctcagtaca aaaggatttg atatcttttc
    36241 agtgtcagat attctactgt tgtatactgt catatcagac ttaagctcgc ctgagcagac
    36301 ttttacaaga gatatctttc ctacaaaagc atctaccatt gtcttaaata ctaaaccgct
    36361 aaatttctca tcagcagaag gattaagtac aacttcatca cttgttccag cttttactgc
    36421 atgaatctta tatttttcag gagacggaaa gtatgctaca agagcatcca ttaatgtatc
    36481 catacctaca tgcatcattg aagaaccgca gaatattggg aatatggaat cttgctctat
    36541 tccgtgtatc aaacctttta ctatttcttc atcagtgaaa agttcacctt cgaaaaactt
    36601 ctccataagt tcttcatcag tttcagcaac tgcttcaatc aaaggttctc ttaactcttc
    36661 agctttatca acatattgag aaggaatttc tgcttcaata actgatttat tttgaagtaa
    36721 atatgctttc tttgttataa catcgactat tcctttaagc tgtccgtctt caatcattgg
    36781 aaattgaaaa ggtacaacac tattaccaaa tttatctcta agttcctcac acaccttgaa
    36841 gaaatcagca ttttcttctt caagtttgct tatgaagaat gctctgctta tattaacctc
    36901 gtttgcatat tcccaggatt tctcaactcc gacttcaaca ccgtttttag cagaaagcag
    36961 tattaatccg atttctgctg ctctacatgc acatttaact tctcctgcaa aatcaaagta
    37021 tccaggagta tcaagaatat tcaatttatg atttttccat tcaactggtg ctattgctgt
    37081 attaatagaa aattttcttt ttatttcctc agaatcaaaa tcgcatgttg tgttaccatc
    37141 gtttacgttt ccgaatctgt ctatcccttt tgtgaggaac agcatggctt cggcaagtgt
    37201 cgttttacca gatccgccat gtcccatcaa tactatgttt ctgatattgg tcgtggtgta
    37261 agttttcatc tatttctcct cttgaaaatt ctttatttta tatcccagta ccattaaact
    37321 gtcactgaga tgaatgttct cgataggtat atcatgtggt aatttcaaat ccattggtga
    37381 aaagttaaaa agtcctttta cccctgaatt tgcaactctg actgcaatgt ctttagtctc
    37441 gcttctcggt agtgataaaa tagctatatc aggatgatgt tcagatataa aatcttcaag
    37501 cttatctata cacattattt cattagaatt tattgttttt ccaattagtt ttggattaat
    37561 atcaaaaatt ccaataatat caaatcctct gtcatggaaa ttattatatt tagctaatgc
    37621 cgttcctaaa ttacctgctc caataataat aactgtaaaa ccttgatctt ttagcaatat
    37681 ttgtttcata ctatcaaata gtaattgtac actgtaaccg taaccttgct gtccgaatcc
    37741 accgaaataa ctgaagtctt gtcttatttg tgaagctgtc atgcccatct tttcaccaag
    37801 ttgcgaagag gaaactttct caattccgtt atctttcaat tcccctatgt atctaagata
    37861 tctgggcaat ctacggatta ccgcttcaga tattctcttt tttgcttgca tttcatactc
    37921 cagtaaaaat aatcactcgc caaattatat ataaattatt atatcatgtc aattgctaaa
    37981 taggttagca caagcttatt agtgtgtctg attattaaat ttaataaaat cttctgtgta
    38041 tcctccaagt ctgcttattg cgctacagat atcgacaatt tctgattttg cgatatcatt
    38101 cagatttttt gacaaatatc ctctcagaaa aactactgct ctaccatcat tcaaatccgc
    38161 cattatgttt gcaatcatgg atttattatc agataaaatg aaatgttttc gtaatagctg
    38221 atatattgaa tcagattcgt ctgtatcctt gtaaactgca actagcacag ataataactc
    38281 attgttaaca ccgtttttat caaagctttc taaaatattt agattcacct tattcttatc
    38341 catcagtgac agaacatcag ctgcttcttc ttttaataac tctgaatctg atgtaacaat
    38401 gatatcaatc agatatttat acaaatcttc gttctcaata ccaataagag aattaatcaa
    38461 ggaagctctg tctgcttctg gcttagattt atcatctatc atttttttaa tgccttcaaa
    38521 cccgattttc ttttgcataa ggtcaattag tgaataagga agatacatat cacaaaccat
    38581 taatgcattg tttaagactt catgtattcc tgattcttct atttgtgttt gaataacatc
    38641 atttattgga cttccttgaa tagaggttgt ttcaaaatgc tttcttgcat cgtttattaa
    38701 cgaaatatac tccgctttag tagtaacata atcgagtttg gcttcacatt ctttttgtat
    38761 ttctttgtta tatttatcaa aaacatctat attcatttta gaaaagcctc gtcatctttt
    38821 atacttttat ttacccttaa cttgtcaatt ccttttaatc ccataaaata ccatattcct
    38881 gaaattaccg gtactaccat atatgctatg gcgaaagcat accatgtgta tcccaatgtg
    38941 tttatcatga aataaaatgg gcctaatgcg catctgaata tataatggaa aattcttaac
    39001 cctaactcta gcgaattgtc aaagataagc agatgaatca gtcctagaag aagataagga
    39061 gtcattgctg ctaatccata taataatcct tttgcaggat gcgcttttgc atctttatca
    39121 tatctttcat attttccaac tgataaagca gtttttgata ccatgaaaaa cataaacatg
    39181 aacatgacaa aagagtataa aagcaaccaa gtagtcatat tattagccat tgacaagggt
    39241 gcaatcacaa aaaaagtgaa aactgcagct gctgttgaat aagcaaacaa tattttaccg
    39301 gcatgcttta aaaataactt catcagctgc tactgcttct cctcttcttt tttctttgat
    39361 tttgcacggt taactaaaat tgttgcacca atcgcccctc ctgctaagac gataatcaat
    39421 attatccacc aatttgctcc tctgtatgat cctggatctg tatgttttat aacagaaata
    39481 ggtgcagaaa tcttatggcc gcaaacagta caaacttttt cttttaaacc ctcttcttct
    39541 tcagtggctt cctttactac aatccaatct ccaaattcat gttctttgcc atcaatacat
    39601 gtcacataaa gatagtcagt gtactgtact agaaactgat tgaaaaatcc ttcaataggt
    39661 ccagaatcta caactgcacc agtatattga ctcatcatat catattcagg gttatacctt
    39721 atataataca tagatatttc atcttttgta taatacacta tatattcatt aaggcctgaa
    39781 acataattca tctctattaa taaaaatggt ttctgactct cgtcaggccc ttgccattca
    39841 ttatcataac ttactattgc agttccataa aaagttgtat tatacagatc tgatccatac
    39901 tgcaaatcaa ctatatctcc atttatagta tactcataat ttttagcagg cttatcatca
    39961 ataatatatt cctgttcttc tattaataca tcatatactt ctccagaagc aaatacataa
    40021 tgcattgatt taacattttt tataagaggc tcatatggta taggatataa aatttccatt
    40081 aactcagtat ctatccaacc taagtatttt ttatctacta aaaatacttc atcagaattt
    40141 gaaaatttag catagaagct gttcttatct attaattctt ttccaatgtg taaaagaacc
    40201 gttttgtttt ctgcagttac ttttattgag tactcaggat tcaacaaacc ataattttga
    40261 tagtcatctt cagaaggcat tacatttaca tactctccaa taagcatctc atatgtggta
    40321 tctatcattt cttgtgctgt ctgagtatat actcgtgaac ctagaggttc agttaatacc
    40381 catttaacat attgatctgg ttctatagaa aattcaagtt ctcctttttt caataaagag
    40441 aatgaagcta ttgtattggc atctccaggg aaaattactt ctgacagaag atattctcta
    40501 tatatagtta gcattccaaa tgaagtatca gatattgtat atatcttatc cactccttct
    40561 ctcattacat aaacattata tgttacatca attacattac caaaatacac tttataactg
    40621 gttccatcct tcaatgtaac ctcgaaaaat gagttgtaat ctataccata tttagataaa
    40681 tcagtagggt tttcctcaat caaattaaca gatatcaaag atgcagcctg tacaagagct
    40741 gtttcaattt tattgtttag ctccttgtcc ggttcattaa ctagcctgta ttgcatttca
    40801 gatctttcat taccttcatc atcacgcaca gtgacttcca taagttcgac tgtagtctgc
    40861 tctcccgaaa tcgaaaacga gtatcgtact atatctgacg atttaatgtt agatggtgtt
    40921 ataacttcgt tgatattcgg attaataacc cttgttttag atataatatc atcaacaatg
    40981 aagtaagtag ctattgctac agccagaata attacgataa ttatgattgt ctttagattc
    41041 ttcatttata agtgcctcct cttaaggtaa acgataccgc caactaacaa tattagtgcg
    41101 ggtaatatat aaagcagcac tgccacgaaa ttagcttgtg atgctgatat ggataataca
    41161 ttcacaaaat agttcttttc ttcaatcctg acctgggtat aatctccttc aagccaatta
    41221 acaagtccca tgaagaactt tgttgaatcc ggttcatatg ctttgttatt tactaaaatg
    41281 taatctctca tgaaatcaga tgatcctaaa acaataagcc gtgaatctgt tgacaggctc
    41341 ttgacagctg caccggcata agctgtacct tttgttgatt catatttgct tattgaagat
    41401 tcctgttttg atattgaact tgatttcaag attggattta cttctaaaaa tgattttgat
    41461 ttattcaata ttctaatact ccttgtatta ggcataagaa gatatgaaat attatcatat
    41521 acattcaaag tagcttctgt tcctacaatt gtcgggaaaa tataatactg attacttgcc
    41581 atatatgaat cagaattgta ttcctttatt ctgtcataat tatactgaac gttataataa
    41641 tcaagaaatt tctgtatatt tggatattct tctcttgtct cagcagaacc aagagctatc
    41701 attagtcgtc caccgttctt catgtaattc tcaagaagtt tgatttcttc atcaagaaaa
    41761 tcaatggttg gattaatcat aagaagaatg tctgcatcct caggtatagc catatcagct
    41821 ctcatatcaa aggtactgac attataacca tttaattcca taacatctct tgctgttata
    41881 tatccgtcta aatatgagta ttcattatgc cccagaacaa aatacgcagt agtatatgtc
    41941 tctctggtta cataacttat agcgctggta aatgcatttt caactttcga acccgtatct
    42001 gttatttgga aagttgattc tgaactgaca aatgaactaa ataaatcata atatttaaca
    42061 gctcttatat tgccatcttt tacacgcctt acaacaaatg actgtcttga taaatcaagt
    42121 gctccagtag gatcaagctg agaaacataa ccgacattta catcaggatc aacatatgta
    42181 acattaatat tgtcataagc atcatagtaa tcaagaaatt ttatgactcc aaaaaaactt
    42241 gatgaagagt taatttttgt agggtccatt aaaccaataa tctctacttc atcctccaga
    42301 ctatcaagaa ttgtcttgga agtatcacct aaagtataca aaccttcagg agttaagtcc
    42361 cattcaatat tcagcaaagg aacggtaata ttaatcacta cgaataaagc aataaccaca
    42421 gctacaatta taaaagagct ggtaccatat ctgaagtttt tacttttaaa tatattgaga
    42481 aacttgttat cttttttcat aattatcacc cctgtaccca acgacgtctc tctatgactc
    42541 tgactgtcat ataaagcatc atagcagtaa atgagacaaa gaaaataatg ttctccattg
    42601 tcagtaatcc tttagtaaat actccatatc tggttaaaac tgatatccac tccataattg
    42661 ctccagcaag accaccaact gttgcggcga attgatctgc tatccacatt ccaaagagaa
    42721 ttaccagtga aataattgct gaaataatct ggttttcagt aagggaggag caaaataccc
    42781 ctattgagca gaaggctgct ccaagaagaa taaaacctat gtaagcacca accagctgag
    42841 ttgttattgc tccttcaaat aacactacaa ctataggata tatgaatgat attcctacaa
    42901 ttaccagaaa aactgtaaat gtggctaaaa atttacctat tacaattcgg ggcagtgatg
    42961 ttggagatgt aagtaacaga acctctgtac catttttctt atcttctgct aagattctca
    43021 ttgttagtat aggaattata aaaattagaa atactccgac atcatcaaga acttcaacaa
    43081 aatccccgta tccagcattt aatgccggat gaaagaaaat tgaaataatt agaatatata
    43141 ctcctattaa aacatatgct atgggggact tgaaatatcc cgctatttct tttcgcgcta
    43201 cagctgacat cttactcctc ctctgtctgt gttatacctg cattgctgac aaccgatata
    43261 aatatatctt ctagtgttgc agctgaaggc ttaagctcaa ttatcggcat tttattttct
    43321 gccattctat agaaaagatc ttctcttata tcaacattat tctttgtttc aagaacatat
    43381 ttatatactg attcttcaga tttagtagtc atcgttatgt acttaatatt aggaatatta
    43441 ccgatagtct taagaacttt atccttatcg cctttaacag ttagatttaa tttcgaaaca
    43501 ccagaaagat tatttgataa tctgtcaggg gtatcctctg ctgcaatctg acctttgttt
    43561 ataataacta ctctttcaca tatcatactg acttcaggca gtatatgagt acttaatata
    43621 attgttctgt ctgacgctaa tcctttaatt actcttctta tttcaacaat ctgtttaggg
    43681 tctaatccaa cagtaggctc atcaagtata agtactttcg ggtttccgat taatgcttga
    43741 gcaagtccta ctctttgcct gtatcctttt gaaagattat ctattagtct ttttctatgg
    43801 tcagtcaatc ctacaagttc cattatatct gaaagttcgc ttttcctttt gttccttgga
    43861 gcttttttga gatcagaaac aaaattcaaa tactcagtta cagtcatatc cttgtacaaa
    43921 ggcggctgtt ctggtaaata tccaattctc ttctttacct gttccggatg agtggttatg
    43981 tcatacccat ctacttcaac agttccactt gtagaaggaa gatatcctgt tatgatattc
    44041 attgttgtgg attttcctgc accgttaggt cctaaaaaac ctagtatctc gccttctttg
    44101 actttgaaac ttatatcatt gacagcaaca acttgtccat actttttgac aagattcttg
    44161 atttcaatca cttggctgcc ctccttaata ataatatgac tacactttta cagctttttt
    44221 attataacct taattgtata ttcttttcaa caacatttta taccatataa tgcttaaaac
    44281 actatttttc acattaatat cacataaatg ttcatattac atttgtgttg tttaaactac
    44341 ccttatttgt tatcatatag taaatggagg actattatgt acgatggaga acatagaccg
    44401 cttatactta tcatattatt ttcaataatt tcggtgggat tttttaattt ttactggctc
    44461 tacattgcta catacgagat caaacaagtt gccagggaac cagaaaatat aaaaccaggt
    44521 gtagaagttt ttctggctat tattactgca ggcttatata taatttactg gtcatataag
    44581 tacaacaaac ttttagcaga cgcacaaagc atgcataaag taacgcctca ggatgtttcc
    44641 cttgtatctt ctattttctc ttttccaata attacattta tcggtatgtg tttcacccag
    44701 caccagctta accgcttatg gaagtatgga acagcaccgg aacagaaaaa gaaaaaaagc
    44761 aagaaaaggt aagaagatgc tagataaggg aaccagaaat agttgttata atgatgaata
    44821 aaaaaatgtg ctttgaagca cattttttct tactggacct ataagccggg ttctgtattc
    44881 gataatcatc tatctaggta cgatatctct atcatactca agccacctct tcgggacgga
    44941 cgggccgccc aatgccccgt atcacggtgt tgctccaggt ggggtttaca tagccagata
    45001 tgttaccata tcgctggtga gctcttacct cgcctttcca cccttaccgt atttctacgg
    45061 cggtatatct ctgttgcact ttccttaaag tcgccttcac cggtaattag ccggcaccct
    45121 tgccctatgg agcccggact ttcctcacat gcagcctttt ggcattgcat ccgcgattat
    45181 ctggtccagt cagtaacaat aattatacca ttttaatgca gtaccatcaa atatatatgg
    45241 aaattctttt tttgtatgta ataacatcca attttggaaa acgtttactt agtaaatttg
    45301 ataaatcatc aataaaacat tcttctgatt catagtgtcc aagttcgatt acagcaggtt
    45361 tttcaaaagc aagtgattta agattatcat attttatttc ccctgtaata aaaacatcaa
    45421 catcaagata ttttatttcc tctataaaac tacttgatga acctgtacct atcgctactt
    45481 tttcaacatc atggggtatt tttcctgtaa tttttacagt atcaaccttt agattagttt
    45541 taattttgtc cagataatca taaaatgaga tttttgcagt gtttcctatc cttccaagat
    45601 aaataccatc ctctaaaaat atattaccta ttacgctgac ttcgcatttt tctgctaaaa
    45661 catcatttag tcttgctgca tcataattag tatgagcaga tataatacca attttatttc
    45721 ttatagctgc cagtattaca tcatcatcta ttctaaacaa agggttatat atgacaggat
    45781 gatgagttac aattaaatcg acttttgatg aagaagcaaa ctcaatagcc tcttttgaaa
    45841 tatcaagaca tatcatcacc ctccttatat tatcttttcc tttaataagt tcaccaatat
    45901 tatcatctgc tatgcttttt tcaaaaggag cgatagaatt aataaattca ataattttta
    45961 ctttcttcat gtattttctc catttctttt tttatcaaag tcagttgatg atatgactga
    46021 ttgctttttt tcataccctt aatcatatct tcgatttttt taagatgctt aataatataa
    46081 ttttgaagta acggatgctt actggatatt aagtatttcg atagaactgc ttctgtatgg
    46141 gtaaaacttt caacttttcc gatatagttc gcagaaaacg cattgtatac ttttgctctt
    46201 tcaagtatca aatcttcttt attgatacta aatccatttt cccataagaa acttctcaag
    46261 tgttctgttc tgctctggca ctgaataaca agttgttctg ctttttttgc gtaatccata
    46321 tggtcgctaa gtatattaat aattacatca gctcccattc cacttataac aatatttgct
    46381 ttttctccaa catgacttat cccatcagaa acaaacgctt taatttcttc agataaatta
    46441 aaatatcgta tattttctaa tgccttatct ataggtcctt gtctgatatc tgtggcaact
    46501 gcttttttac atactttctt ttgtattaag tatattggta tataacagtg atctgtacct
    46561 atatcataca ccacttcaca tggctttaca cagcggctca ccatcatcat tcttggactt
    46621 aatagagatt ctatatcttt cataatatct attctacaac acttagtgtt tattttgtag
    46681 aaaaagatag caccaattca agttatctga tactatatat aatctaaaca ggtaaacagc
    46741 taaaattttt aaaaagttgt tgacctaaat tatacttgta tgtataatgt atcaggaatt
    46801 aagatttccg ggcctttagc tcagttggtt agagcaaccg gctcataacc ggtcggtccg
    46861 cggttcgagt ccgtgaaggc ccaccaaaaa cctgcctatt atggcaggtt tttattttac
    46921 tcttttttac agtttgtatg tggttaaaat cacaaataag ttaagactgg ctttttgtta
    46981 ttttcaagag gtataatgat ttttttatca ctgaattcca ttacaagaca tttattctct
    47041 acatactctt ttgatgcttt tggcattccc ttaataattt gatataagag ttcatcctgc
    47101 tgttcatcat attcttcaac ttcatctgat gtaaaaagaa gacttccata tgcactattt
    47161 atattgccta agatatactt cattgtatag tccataaagc aattctgact tcttatcata
    47221 aagacatcag gatcatttaa aaataccctt ttatcaaggt gtttacgaaa aacagtattt
    47281 acaacagaat ttctggttga gactctttct cttactgcta actgcatata tcttttgtta
    47341 tcccattcta aagatatatc agcccctatc ctacaaaaat cgacattcca aaatgctggg
    47401 cctaatggga ctccgcaacc aagaagtatt gcatctttgc aaatatcgtt tataaaatcc
    47461 atagcttcac acattaactg tccccgagtc ttgttgtttc ttggtaatat acatgcacta
    47521 tataagaaat ccaattttaa aaaagtgaaa ccccattctt ttataactgt atcaaataca
    47581 atttcaagat aattttttac atttctgtta tctatatcaa gagcataaaa cccgctccag
    47641 ttagatcctg ctttgtatgg ttttcctttt gcatctttta ctatccagtc tggatgttct
    47701 ttaaataatt ttgaatcttt ctcagccgaa aatggagcta accaaagacc tggcatcaca
    47761 tttttgccac taatttttga agctattttt ttcattccta taggaaattt ttctatgttc
    47821 acatcaagcc agtctccgac aaaattctga tatccgtcat caatctgaaa aatatcgcag
    47881 gatggatttt tttctgtgta atagtaaaga ttcttctgaa ctttgcgttg tgatatctcc
    47941 tgataataat tataccaagt agtaaatccc ttatattttc tatcaatgcc atctaaatat
    48001 gagcttctta ttgctgaacc tgtctttttc tgcctgcagt tatcaaaata tctgtcaaat
    48061 accccatcat aaccgccttc atcctcatat aagctgaaca tatatttgtt tacattttcc
    48121 aacccctcac aatctttttc tattactatt gcattcttat ttgcatcatg ctgatatatt
    48181 gtaaaccctg atctttccga taaagaaccc agtaacataa tattactcga gtttttatct
    48241 tctttaatat atgtatatgt ataacttaag aaattaccct ttttaccttt agcttttatc
    48301 ttatcctgac cgttatatct atccaaagaa tagtgtttat ttataaggtg ccccaataaa
    48361 gcaaggtttt cacttttttc atctatttca tactctctgc ttactgacca gctttgatat
    48421 ccgttacaga aaactcttga atttttagaa aaattataat gcttttcgat actgacatga
    48481 tgaagcgtaa caccatcagg aaaacggata atatatgtac tactgtttcc agatttgatt
    48541 atttccagct cacagtcatt aaaaacacca ggttcattga attcatgaac gatatgcttg
    48601 ccattattac catgtgaata tactactttc agacaattat tatacatttc agccccttta
    48661 tcattataca gtaaatacaa taaatgtacc atactgagaa tgttgatttt tctattctca
    48721 tagtatatta taggacttaa gatttttaga atggaattaa cattttggat aaaataaaag
    48781 gttattttac aagtaacgct cgagttttaa tatcatggtc cctttttatc atctggatga
    48841 tagttatttt tatcctgtct gctcagaatg ctgaaaaatc ttctgataca agtggatctg
    48901 tacttacatt catattgaac attacatacc gtggctttaa ggatatgaat ccatcagaac
    48961 aatttgaatt actggaaata tatcaaggtt ttattcgtaa attcgctcat ttttttattt
    49021 ttggaatatt aggtgtactg gctgttaatg catataaatc attaaaaata agagataatg
    49081 gaaaagtagc aatacttgct ttcgcaacat gtgtaattta tgcgataact gatgaagtac
    49141 atcagttatt tgtaccagga agagcatttg aatttactga tataataata gacagttccg
    49201 gctctttcat attcatatct ttatcgcttc tgattgttta cttgattgaa aacagaaaag
    49261 aagctcaaag tgatatgcca tagagcatca gaaaatagaa cagcattagc tgtcctataa
    49321 tatctgataa atatttggta ctatttttac ataaatgaaa aagtaaaagg tttattcaag
    49381 ataatcccga agtttcttac ttctgctggg atgtctgagt tttctcaatg cttttgcttc
    49441 gatctgcctt attctttctc tagttacatt gaactctttg ccaacttcct ctaatgtcct
    49501 ggctctgccg tcatcaagtc caaacctgag tttgagtact ttcttttctc tgggggtaag
    49561 agtatccaag actgtcataa gctgctcttt aagcaatgta aatgcagctg catctgcagg
    49621 ggcaagcgca tcttcatcag gtataaaatc acccaaatga ctatcttctt cttcacctat
    49681 aggagtttcc agagaaacag gatcctggtt gatcttcatt atttccctta ccttgtcttc
    49741 tgtcatattc atttccctgg ctatttcttc aggtgtagga tctcttccaa gctcatgaac
    49801 taactgcctg gaaactctgg tatatttgtt aatagtctca accatatgaa caggtatcct
    49861 tatagttctg gcttgatcag caattgatct agttatagct tgtcttatcc accaggtagc
    49921 atatgtacta aacttgtatc cttttgtata gtcaaattta tctactgctt ttatcaagcc
    49981 aagatttcct tcttgtataa ggtcaagaaa ctgtaatcct ctgcctacgt atcttttagc
    50041 tatacttact acaagacgca gatttgcttc agcgagctgt cttttgcatt cctcgtctcc
    50101 tgcttccatt ccttttgcta aagcaacttc ctcgtcaact gttagtagag gcactttacc
    50161 tatttccttt agatacattc tgacagggtc atcaactgat acgccatctg gtactgttat
    50221 ttcgatatct tctgtctcat aatcgacttc cgatatgtct ttttcatcat caacaacatc
    50281 aataccagct tcttcaaaca tctcaaaaag cttatccatc tgatcagcat taacttcaat
    50341 ttcatcaaaa ctgtcagtga tttcttgaga tatcaatgat cctttcgctt tccccttttc
    50401 cataagatcc tgcatgactt tgtcaaatgt gagaggagta acctcttctt caactgcctg
    50461 caatttttct ttttttgctg tgttagcttt ttcttccatt tttcctcctg ttaaccgctt
    50521 atcttgtact tgccaagcat tgcggtattt cttctcaatt catctttcaa tttttctttt
    50581 tcctcttcag tcactccctc agatacgagt gccttactaa gaactatatt gttatttaca
    50641 attatgtcgt gtctatatct tgctatataa tctgataaag ctttattcgg atcagatata
    50701 gtatggtatt cagcggcttc ctttataaaa acgcctcttt gctcatcgtc gagcattgct
    50761 gatatttttg cttcatctgg gctttccatt gaatcatatt tatttaaaat ctcttctaga
    50821 agttccttgc tgaaatcatt atgagtcatc cctgcctcaa tcaactcttt tcgtatttca
    50881 tctttgaatc ccggattgtt acataaaagc actaatattg aatagaaaat tttatcgagt
    50941 tttttttcag tatatctatc tcttagtgaa gaagctattt ttgtaaccgg ttttgctttt
    51001 atctgattgt attgagtatc actgtcgtct ttagtcctgt tttgaacctc ctttaaaagt
    51061 gcatcataac tagtgttaaa tcttgctgat atgtttttta tgtaaacatc cctttctacc
    51121 ggactttcta cctgtccgat tgctgatgcg acacctttca aaaaagcaat ctgtccttca
    51181 gatgtattca aatcacaact tgaacgcaaa agctgtgctt tgtattcaac aagcattaat
    51241 gatttcttaa ttgcctgttt aaattcgtct ttgcctttgt acttaatata atcatccgga
    51301 tctttaatac ttctaccatg cttatcttct ttcggtaaaa caagaacctt cacaacacat
    51361 cccatattag caagcatatc taatcctctt aaagaagcat tctgcccagc tgaatcatta
    51421 tcatatgcta taaccgcctg accgaatatc cttttaataa gcttagcctg attatcagta
    51481 agcgctgttc ctaaagatgc tattgtgttt gtaaaaccag cctgatgaag acttatgcaa
    51541 tccatgtaac cttctacaat tattacataa tcctccctgc tgtttttagc aaaattcatt
    51601 gaaaagagac ttttgctttt tttatacacg catgtttcag gtgaattcat gtatttgaca
    51661 tccttatcat ccagtgctct accgccaaat cctataacac ggtttcttat atctattatt
    51721 ggaaacatta tacgatttct gaacttgtca attgttccat tttttgattt agagcctaaa
    51781 ccagcttcga taatttccgc ctcactgaac cctttctttt ttagaaattc cgtaagtttt
    51841 gtccaatcat ttggtgcata tcctatacca aaggaaacaa tggtatcttt ttcaattttc
    51901 ctgtttataa ggtactgtaa tggttcagga ttctctttag attttaaaca gtcaaagaag
    51961 aatcttgcag ctatactatt catctcatat gtgcgctttt ctttttttag cgttgcctca
    52021 tcaacataac tgttcttctg aggaatatta atgtttgctc tttcagctaa aagacggacg
    52081 gcttcagcat aatcaagatt ttcaatatcc ttaatgaaat taattacatt cccgccttta
    52141 ttacatccaa agcagtaata tatctgcttt gcaggcacaa cagaaaacga aggtgttttt
    52201 tctttgtgaa atgggcacag tccgaaataa tctttccctt ttctgtcaat cttaacatat
    52261 cgtgatattg tatcaacaat gtcatttttg gatatgattt cttctattaa cggttctgga
    52321 taaatatatc tgtcagccat tgtccctcca atatattatt cgacgccgat ttgtgattcc
    52381 ctttatttat tttgtctgtt caataataca ttatatcata ttatcatgct aagcatcagg
    52441 aggttattat gacatcatac aaattaaaaa gatcatcccg gttagcagat gaagaaaaga
    52501 atattttgga catagattat tttataatcg aagaatttaa aattagaacc aatagcaaat
    52561 catatggaat aaaggttgaa acaaagaata aaggccaaat aaccgataca tgtactgtaa
    52621 atgatttaac agctaatctt gacgaagcaa tgtatataat aagtaaaatg gcagacaata
    52681 atgtgtctgc aatagctgca aaagacgtca ttgaggatat ggtttactga atcttttttc
    52741 ttggtttatt cggtcgcaat acagatacag ctttattatc ccagctgata tacttattta
    52801 cgatatcgag gatatcctct gctgtaactt tttctgtctt tttaatggct tcttctcttg
    52861 taatgggtct gcctcttaga agcagattcc tgccattaga catcatcctc gaatccgttg
    52921 tctccatacc taataacatt ccagttttta tctggtctct tgctcttgta atctcttctg
    52981 aatcgaggtt attagaacag atatcataaa taatatcctt aatacactcc atcatctcat
    53041 cgaatcttga aggattacat ccagcgtata tatttagaat tcctcctgcc tgatagaatt
    53101 catttgatga gccgatgttg tacacaagag ctttttcttc tcttacaatt tgaaataatc
    53161 ttgatgacat gctctgacct aatgctcctg acaatactgc aaagggatat ctttcatcgg
    53221 agtcatgcaa tgcaccctta aaagccatgg aaagataagc ctgctctatt ttctttgctt
    53281 taaaaatatt aacaggtctg aaaacagcta atccaccgtt gtttgcttta tgacattcgc
    53341 cttgtggata atcggaaaaa tagttttcaa gcatatccag aattacctct ttatcaaaag
    53401 atcctgaaac agaaataact gtgttgcatg aaacataaaa ttctttcata tatgaaacaa
    53461 tagtgtcata tgaatatttg cttactgttt ctattgaacc tagtatcgga aaagataatg
    53521 gatttccttt ccacatctcc tcttcaagcc ggtcatggac atagtcttcg ggagaatcct
    53581 catacatagc aatttcttcc attactactt tcttctcatt ttctatcatt aactcatcaa
    53641 actttgattg aagaagcata tcagagataa gctctaaagc gacacttaaa ttatctgatg
    53701 ttgtttttat atagaaacaa gtcatatcct ttccggtata tgcattcagc attcctccta
    53761 tctgatcgga tatgattgct atgtttttag cgcttttgct ttttgtgcct ttgaaagtca
    53821 tatgctcaat aaaatgagag atacctttgt tgtcttcatt ctcataatta cttccggctt
    53881 taacaaaaac acctatactg attatattct ttgcgggtag tttctcaaaa atcaccctta
    53941 taccatttga aagtttttca atttcaatca tattgtttct cattaaaaaa gcccggtgtt
    54001 acccgggcat tgttttactt ggataacaga gcttttctgg aaagatttac tcttccctgt
    54061 ctatcaatct ctactacctt aacatcaatc ttatcaccta ctgataatac atcagatacc
    54121 ttattaactc tctcatttgc aatctgagat atatgaacaa gaccttcttt gccaggaact
    54181 aactcaacaa atgctccaaa ttccattatt ctagtaacta ctgcatcatt aaatatcata
    54241 ccagcttcaa gatcatttac aataccatta ataattgaaa ctgctttatc tcccgattcc
    54301 tgatctacag taagtatata tactgttccg tcatcattaa tatctatttt aacacctgtc
    54361 atgtcaatta tcctgttaat gacttttcca cctgtaccga taacatcctt aattttttct
    54421 ggatttatgg tcatagtaat tatcttaggt gcatatggtg ataattcagg tctaggcttg
    54481 tcaataactt taagcataac ctcatcaaga atgtatattc tgcctttttg ggttatttca
    54541 aatgctttct ttataatctt aggagtaagc ccgtcaactt ttatatcaac ttgtatagct
    54601 gttattcctt tatgtgttcc agctacttta aaatccatat caccaaagaa atcttctatt
    54661 ccctgaatat ccatcatcat ttcaaaatta tccggatctt cttcatcagt aacnnnnnnn
    54721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    54781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngcttccc caacagaata tgcagggaaa
    54841 ttatagtgat ggatgtatct atcttctgtt tcagcttcat caagtatttc aagaggaaga
    54901 acctctctca ttccacctaa agtaacactg gttaatacct gtgtctggcc tctttggaac
    54961 atagctgacc catgtgttct tggaagaaca ccaacttcag agtataaagg tctgatttca
    55021 tctaaacttc tgccatccac tctttttttc tcgtgaagca gataatgcct tacaactttc
    55081 ttttccagtt tatataagga atctgctata taacttggat tatctttgta tttttcccct
    55141 aactgctcat taacttttgc agtaagttcg cttattttcg tgtctcttac agttttatct
    55201 actgccaata cagcctgttt catttcttca ttagcaatac ctgctacttc atcataaact
    55261 tcctgtggaa ctgctgatga tgtataagaa aatttttctt taccgatttc ggatactatc
    55321 ttttcaatct gttcacagac tttttttatc tcttcatgac ctttcatcaa agcatcatac
    55381 ataatatcat ctgaaacttg tttagcttca gcttcaatca tacagatctt ttcttttgta
    55441 ccagctaatg ttacatacat atctgaaaca gctctttgag cagcatctgg atttattatg
    55501 atttcgccgt taacaagccc taaagcaact gctgctactg gtccgttaaa aggtatatct
    55561 gaaatactag ttgctattga agctccaatc attgctgcca gttgaggctg attatcctgc
    55621 tccactgata aagctgtaca tattattact acatcatttc ttaagtcgtg tggaaaaaga
    55681 ggacgcatag gtctgtcaat gactcttgat actaatattg cagcatctga aggtctgcct
    55741 tctcttctat ttctgcttcc tggaatacgt cctattgaat acatcttttc ttcataatca
    55801 atactcaaag gaaaaaaatc aattccaggc cttggctcct ttgaagccgt tatagtggaa
    55861 agaaccactg tgtcaccata ccttacaagg catgaaccat tagcaagctg ggcatgcttg
    55921 cctgtttcta tgattaattc tttgcccgca tagttaatta catgtttttt gtgattttcg
    55981 tacattattt atctcctttt attcttttcc ggagatattc cacttgaaaa cagatattaa
    56041 aacccgccat atttcggact ttaatctcgg tttttaagga aataccgccg gacatatgca
    56101 aaacggcgaa aaatcgccgt atttgtttta ctttctgagt cctaattcct taactacggt
    56161 acgatatctc tcaatatctt ccttcatcag gtaattgagc aatcctcttc tttgtcctac
    56221 catcataaga agtcctcttc ttgaatgatg atctttcttg tgagacttta gatgctcatt
    56281 cagatggttt attctctctg tcaaaatagc tatctgcact tcgggtgatc cggtgtcccc
    56341 ttcatgcaat tggaattttt gaataattcc ttgtttcttt tctttttcca tatcatttct
    56401 ccttttctat tatcgccata agcaatgtaa aaggtcggag tattccaaac acatagccta
    56461 aggttctttg ggtatattac cattttatgt tctgcatgtc aaattattga ttatgatatt
    56521 atgtattaat atataataga ttttattgca gtcaaaagtt cactagtaaa aaaaagagca
    56581 tatgctcttt taatctttta cttatctgct acatatggca gcaatgcaat ctgtctggcc
    56641 ctcttgatag ctattgtaag ctctctttga tgccttgaac atgtacctgt aactcttctt
    56701 ggcaatatct ttcctctttc agaaacatac cttttgagtt tgttgatatc tttgtaatct
    56761 atagattcat ctttatcagc acaaaactgg caaacctttt ttttaggttt cctgtattta
    56821 aaatctttcc tatcttctct atccatgcga tccatatcag ttcgctcctt tcattcttat
    56881 cttgctagaa tggcaatgcg tcatcttcag agatatcgac atgagtgaaa tccatatcat
    56941 ctccagcatt gtttccggtg ttatcactgt cattcgagtc ttttttacca tctgcaaaat
    57001 atgtttcatc aagtacgaca tcagtggcat atcttttttt gccttcctga tcttcccatg
    57061 aacgattctg cagtcttcct atgacgacaa cctgctgccc ttttctgaaa tacctgttta
    57121 caaactcagc ttgtttggac caagctgtta tattaaagaa atccgcttgc acttgagaat
    57181 ctttagctgc aaatcttctg tttactgcta atgaaaatga tgctaccggt gttccgtttg
    57241 aagtagttct aagttcaggg tcttttgtca gtcgtcccat taatatgact ttattcatat
    57301 cttcctccta taacttgatt gtttggcctt ttatgatgcc ttcagtaatt ctgtacaatc
    57361 tttcgagttc ggaaatgaag tccggttgtg ctttaaacgt tactaacaca aaataaccgt
    57421 ctgttttgta attgattgga taagcgagct tctttttgcc ccattcatca accttatcaa
    57481 tttcacagcc agcatcagtc atctgctgta ttaacttttc aactgtgttc ttttgggctt
    57541 cttcttccaa tgtacagtca acaataaata ttgtttcgta ttggttcatc tggtttacct
    57601 ccttctggtc taatcggccc cttctaaacg gggcaaggaa tcaatgcggt agtattgtac
    57661 tacaccatta tatcttaatc aaggattatt ttacatatgt gcctcctttc cgataggaaa
    57721 aaagacaata tcatattttt ttgtattaat taacagacag ctcttcttga aatatcagga
    57781 acaaaactct ttattgcagt aaaaccgcct tttaaggtat atttttccat agatgcagga
    57841 atcaaaactg actctcctaa gcccactcgc acaccatcta cttcaaactc cccttctatt
    57901 actgtatatg tgtggaatga tgaatactga gtatccgacg aatactgtaa atttgacaca
    57961 attttttcaa cagcaaaata cttattgtat acaagataat caacaccttt aaattgtaca
    58021 gttttattaa tttgtatttc aggattatta aaatttatta catccatagc tttttcaata
    58081 tgaagtggtc ttttctttcc atctggtcct attctgtcat aatcataaac cctgtatgta
    58141 gtattagaat tctgctgaac ttcaataata attataccct ctccaattgc atgcaccaaa
    58201 cctgacggaa tgttaagcca gtcacctttt ttaacattaa caaagtttaa tgtctcttca
    58261 atacgattat tttgaaccgc atctgcaaat atttcttttg taactccttt ttttaatcca
    58321 tatactagtt ttgcgtctgg ctttgcatcc acgatatacc acatctcatt cttgcctaat
    58381 gcaccatcct cattctcata agcatacaaa tcatcaggat gaacctggac cgacaaaagt
    58441 gaacatgcat caataaattt gataagtaat ggaaactgtt cttgtgaagc tgttccacac
    58501 aaatccttcg gataccatct gaatacgttt tccagtgtag catccttcaa atcaccattg
    58561 cttactatgc ccattccatc tttatttgca gaaatttccc agctttctgc tgttatggga
    58621 tattcaagtt tcttgttcag tttcaaaaaa ttattaccgc cccagatata atctttatat
    58681 accggtttga attttaatgg atataacata gacacctccg ttttatctgt tataaattat
    58741 atcattaatt c
//
LOCUS       DAOSXM010000004        56272 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2111
            NODE_361_length_56272_cov_10.526673, whole genome shotgun sequence.
ACCESSION   DAOSXM010000004 DAOSXM010000000
VERSION     DAOSXM010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864178
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 56272)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 56272)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:24:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,261
            CDSs (total)                      :: 2,229
            Genes (coding)                    :: 2,218
            CDSs (with protein)               :: 2,218
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 3 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56272
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_361_length_56272_cov_10.526673"
                     /isolate="AMR_MDS_2111"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2257
                     /locus_tag="PLQ68_01280"
     CDS             <1..2257
                     /locus_tag="PLQ68_01280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK71309.1"
                     /translation="DLAASLVEALALETTEGTKLIDAMIADGIVTEEEALEAEVKDDE
                     PVADAVTVAVTGVKTIKVTFAEAQDADTSVFVLKKGAVGQALKTPSWDAAKKVATIEK
                     AIAFTKGDYTITANGVAVDFTVEANETATELVASASTVYAKAGAQDIKVNLLNQYGEK
                     MVFGVGNLVYGASLGTLAEKDNNTLTLTLPATLPKAGSTSTIFAYYTPTGFSINTTIT
                     VLEDQVLTSIVFNGPITVGDECKDNYNSRLTEKTAGNKLSYKAYDQYGNALSLATGEG
                     TTYQLVVSGGSVASKDGKTMTLTGLKKGTFSIRAIALTTGNISDLYTETVYAKPALAS
                     YDITLPEAIYQNEAAKFTIVAVDQYGKAFNVTPDATTWSVTPLSTTITAAHMTVPAYA
                     KNEISYTITGKGNADLYFTTGSLTVTKSVVVQPEKVISSIASVPVSTEALLMGKTIAF
                     DLSKVVLNDQYGNKIAYDASVWSYDLRRLADTAPLGNTATDTNGTALVLTAPVGTATS
                     YAIKAHLEGTDQLQLTIYKGGAYGSTDTNNYVSYTYDFSLASVKAASVVAYELSVKEE
                     MYAGKTTTEMSATHDLAVALIGKTANGTTVTLVDSSSTALPSDTISKYTTTGANVTLA
                     TATSKLQVTTAVTGTADGTTTIRAWNNKGESVAEAAVALRRAAPKVDTLTLTYKSGVA
                     TLAAKDQYGITITPPTGKFFSSNSDLTIGAVTVATDKTTVVINGAAGKTAVIRFVSDD
                     GVWAREVSVTK"
     gene            2367..5090
                     /locus_tag="PLQ68_01285"
     CDS             2367..5090
                     /locus_tag="PLQ68_01285"
                     /inference="COORDINATES: protein motif:HMM:NF020730.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK71310.1"
                     /translation="MKRTLSIILITVILLSYCAIQPKAYTSVEIAYGLNLLRGTGEGL
                     DEAYLSMTPMRTNAAMIILRLLGLENDALEHEKISTFSDASSATAYWQPILAYLYDNP
                     DVGFSGYGDDTFRPNESINSQMLAKVLLTVLGYKQDVDFSWAETLKFADSIGLKSLVN
                     KSTITNNDVAIALVEALSIKTKGGYTFVTELVVADIINIDQAEKYGFTVETTQVEIKN
                     AKATGVKTITVEFLMPIPKNSTVMLRKDFIGISNTYTLSENRRILTINTTATMTPGQY
                     TVAVAGSVATFEVFNEKASSLVISTERIYKDVLQNIGLKLLNQYGEEMPITNVNISVT
                     NKTSGSRKITVTKIDNEVYLDVSYANLADQIYIYALDNSTLLNDSALLTVQSVPSIKK
                     ISILGVEASSDTNRIYEDTSSHIIKIQAYDQYGQKYFIRQSDIDKKNLLLISSNNLCI
                     SPSNIKADINGNLVFSTNMAGEVLINIIVSAEGISASTTIAVYAAPVLSSIKVDQLPS
                     TIFKNERVEVGVIGYDQYGSIYEINATDIIGYTTTDPMIVPAASISVKRGVLSFNTLN
                     SGEVSVNSRINNSLMPLFTIKVNDGNVPYSITSLDIPFSHFEKGIENIQIPLSNIKVV
                     DQYGNPNSLNERPVWDTVEWGIYIDKVSGNSFLYENGVFSTTNTVGTDVFNVYITRDG
                     RIMDRSGYSFMLTNVSASEIQVFEIDIPKIIYGGANNRIEAHTKYITIKGYTLSKNPV
                     MLKTDDLGMPTLISAITVSNSKVYIDTSTWAIKFNTYFENDDIVTVKFWKDGMEIESE
                     DIVIMATESKVSRMEYDQAQSFIISSSEYYTLPLNIYDQYGIETNLPENTQWVTNSNL
                     IDSITFDPSTKRLRIVIKGSVISETEVYISYIAPDGSFSFRGPYTIMPGRY"
     gene            5138..5824
                     /locus_tag="PLQ68_01290"
     CDS             5138..5824
                     /locus_tag="PLQ68_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXK71311.1"
                     /translation="MGHKILVVDDEKNIVDILKYNLEKEGYDVICAYDGKDAIFQALK
                     HKPDLILLDIMLPEYDGFIVCRKLRESMTVPIIMLSAKGEELDKVIGLEMGADDYITK
                     PFSARELIARVKSNLRRVTYANNDNSEITYLKLRDLEVDEAKCEVLKEGKVLDLTARE
                     FELLVFFMRNPEKVFSREFLLDKIWDYEYYGEMRTVDVTVSRLREKLSDDPNEYIVTK
                     RGMGYYFKKD"
     gene            5907..7595
                     /locus_tag="PLQ68_01295"
     CDS             5907..7595
                     /locus_tag="PLQ68_01295"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXK71312.1"
                     /translation="MNNNVEQYHYNQFVRNIENGVNMLSYNENEELDIYTVYTDMRDL
                     YAPSFGIYGSNKSYTIVDRYSNEIYSNDSVFLSSTDSSFINELFLSANFMSSMAGSPI
                     NSNATLNLRGRTFYDYAITISNIVVYFRYYKADWQDMVTEFNETLLSALLFAIIIAFI
                     VGYLLSTAITKPIKDMTIRTKAIAAGDFGKTLDIKADDEIGQLSSSINNMSYSLQNML
                     DEISSEKNKMEIILDNIEGGVIAFNLKGEMIHNNPAAYIMLNRADLDFTFENFVKEYN
                     LDIDVSDIPADLYKESMLNIDDETLTISIASFKDSLGNAEGVIILLRNITKQFKLDQM
                     RKEFVANVSHELKTPLTSIKSFTETLMAGVDDEETRNHFLEVINSESDRMNNLVQDLL
                     QLSSMDMGRMVLKISHHNVSSLITDAMRAVEAEANHKNHTLVSDIQYKGIAYFDYDRL
                     LQCTINLLVNAIKYTDEYGKIEILTYEQEGYLFISVRDNGIGISKKDQERIFERFYRV
                     DKARTRIMGGTGLGLAISSEIVKAHNGQIEVNSKVNQGTTITIKIPLTQKEDEQ"
     gene            7592..9028
                     /locus_tag="PLQ68_01300"
     CDS             7592..9028
                     /locus_tag="PLQ68_01300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK71313.1"
                     /translation="MKRKIAYPILIILLLATVFQSFMLINYHSTGRLLPRRTIEGPNT
                     VTEVINFEEYLLESYIRPFEITMTTGEGLNVNKVPRDLQGYFDQIWKDTISALATLAD
                     KKLYEIYDEDKWNEITGSAGYTIKLGYDCPIRFINWVTGSVNSNSEIDSINKIMVIPI
                     DSDEGDVYIRSGNKVFRYLNVKLSGLLKQNEFLSLYDEIKQQSEITYVYLNEVALYEN
                     FKGNIEPDATVIIMDDYVTINRIRVASYEILSECMFNLQIEPDVNNLSPLVKQYISEI
                     KTKLFGIYSDVYKEVIAQNRELTFSDQYNSYSIHTDGSLSYRYASASYIQEKGDISLA
                     FSNALSMLTDMTSLSGTKQNNLVLTKVKEQDDSYAFSFTYFFNDYPVLMQNEKYSVVI
                     KATADRVISMDANLYDITDASVAGSYMDLYDLRTFNMLSEQSISLGSLKADNMYIGYI
                     KDEDTVLLPSWIIDDESVLVIELKEAED"
     gene            9028..9804
                     /locus_tag="PLQ68_01305"
     CDS             9028..9804
                     /locus_tag="PLQ68_01305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK71314.1"
                     /translation="MDSKKALRIIIILLLVVNIFMTIYITNLAFSEPKSEDELKHVIQ
                     LLEYRDIKLECDIPQYAAPSAELIYIEADNTEILNYLSNIEGLLYEDEASKQIIFEPV
                     VTQRYENFSLDLATQLSDSFVENLPVKSESYMLDSILTAGVNIYKFSYIYVDGSSYIY
                     DKKIEITISRDGIDQVIISNVNYQVSDTEYNRATVSIGNILASGFIRQGQSFITIKKI
                     DAGYLYDDETSSIMNVWRVMYNNGQERYFSSVDGTIINKN"
     gene            9936..10442
                     /locus_tag="PLQ68_01310"
     CDS             9936..10442
                     /locus_tag="PLQ68_01310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HXK71315.1"
                     /translation="MKKEDEFSLDEFDLENFEIEDFKFDKSEYDKQCKQQQLTNSEYL
                     EIFENDMKKANLAKSTIRRHRSNVDFYINTYLLREDSLKMSDGTFMIDMFLGYFFIRK
                     CMWSTPGTIKTTAASIKKFYKCMAEHGYIENNSYNYLCNEIKENMESWQRECNEYNNG
                     SEYFYIDE"
     gene            10466..11812
                     /locus_tag="PLQ68_01315"
     CDS             10466..11812
                     /locus_tag="PLQ68_01315"
                     /inference="COORDINATES: protein motif:HMM:NF014825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C domain-containing protein"
                     /protein_id="HXK71316.1"
                     /translation="MTEKQAKRIFKKFNNASDVIRCPNGKANTKKLLHSYARAAVNLY
                     GIISCNELLDIFNEQNNEHIEYKDIYTFLLPLVLKEGWYCFYKHYIVHYSFFTDFEQA
                     DYLLEIQSEKPRYIPDRSTFLQYADEYYTDNDNLLNIYLYISDHFGRTKHIYQIFNEI
                     EEYIMYSNDIGGLTDILQKIDFMVDDEKQIQDFINMITDAKNNTRIWENKGYTPIELC
                     DIMKEQNKKIIKFPVYEKRKIERNELCPCGSGKKYKNCCSVFEDKKEAQLSSEECREF
                     YEMWYGLLSFVNDKKNILNIRITTDYPNNINDVKLHKVREVLWSNPELIEEYIKKVAL
                     TKEKIDILKLWKTNHIKGMFFVLEYKPEYAVAITSNENGEDRLYGIKGISNPISNILQ
                     RNLPIQIETVLLPFKGKIIYDTYIGTFQIKYLEGVKKFFKEIYDKAIEYGIITCLE"
     gene            11955..13136
                     /locus_tag="PLQ68_01320"
     CDS             11955..13136
                     /locus_tag="PLQ68_01320"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HXK71317.1"
                     /translation="MDLYSNLQNRLNAMKQGGTYKEYQYLTTPMSSKSNIEKYNDVIV
                     LCSNNYIGLCDNKEIIQAGHDALDKYGVGAASVRFICGTYDIHRQLEQKTAEFMHTES
                     CLTYMTCWNANTGVIPAITFEGDCIISDALNHASIIDGCRLASKKVERFVYKHSDMED
                     LERLLKESDRFNTKLVITDGVFSMEGDIAKLDEIYVLCKKYGAILMVDDSHGMGVIGD
                     TGRGAIEYHNLLGKIDIITGTYGKALGGSAGGFVAGRQEVIDMCIQASRTSLFTNSLP
                     PVVAAMALKSVQILERDPSIIESLHKKVKYLRQRLHEEGFEPLEGDSAIVPIIVKETS
                     KAIKIAKAMLDKNVYVTGFGFPVVPEGTARIRIQVSDALSYEDIDYAVKAFKEVFDSL
                     D"
     gene            13201..>13865
                     /locus_tag="PLQ68_01325"
     CDS             13201..>13865
                     /locus_tag="PLQ68_01325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXK71318.1"
                     /translation="MIFSNSIKSIVRSKGKTVLFTILIFSLTLALAICVSVWAGIEMF
                     LKECDEFYTTVGVFEYIGTEYPNDTIYDSYMDETLDGFDEDIITNHPSVLMWDSSRRS
                     LGYIDGFWRTDQYMPQRMLSVVIVGNVSDSKDEFYTAIVMKVGYSLLIEEETVIHIDK
                     DFGPLESNHYYMLFGEAYYARTPIINFARTDYENAIANELGLSIPYKVDITKDNNGFT
                     TFE"
     assembly_gap    13866..13965
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <13966..16295
                     /locus_tag="PLQ68_01330"
     CDS             <13966..16295
                     /locus_tag="PLQ68_01330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXK71319.1"
                     /translation="STKDISSIYSFNQNELYILEGRVFNEEEYENSEKVCIISQLMAQ
                     RLNVGISDKINLSVSTSNHPKLDESYWASTGFDKSDEYEIVGITNATGDMFWYVYVPW
                     SENMPYSSYPIGYTIGQAVIENGQGAQFYKDIEPLLKDRVRLTVYDQGYSSVEVPYTT
                     LLNVVKILTAVFVLVELAVVVFFGYLFVYRQKETAETMFMLGSGNTRVFSYFLFSSGF
                     IALAACSFAMVGGRLLSGLIMSAVRKIAEYFSLIDFRFSNANLSISKTLEFNPDIGWD
                     IFIMVSATVLISCVISCLVFLTLGFLNKSGKTKKIVGPKKEKKSMNNPSKSAKYAFLS
                     ILRGGPRTAVVPMLALTVVLFFGQLSVVMQSSNERIEYIYNNNVVEGYFTDYKGKQIA
                     KQNISAYNIANLQHSGYIEDLCMTVSEPSYYVGAVIKDGKISDIEPLYVPSNAFVMES
                     LQAQISRGPNITFTNNIEKSPEFYYVDSIDIDYVEGYDESVFSAFESEETSICLVTQS
                     YMKEHNLSYGDVIRLAVDNRINSLKYNGRIFQHYDLKIIGSYVKHGDKDTIYAPLSEN
                     FNTSAILLKADISDNQDDFDNIQTITPQQKKYLLDVNLNSVTFKIINTKSLSEFKDFS
                     GVYGFSQVNRISTIREFMVLKDAILNSSLANVEQQIRYLNIAYPILYVLIAVVAYAVS
                     YLMVVSRKKEFATMTGLGAEKKKVFMSFFTEQTLLCFIGTLAGIVIWYILFNQLSLMH
                     LVLTAGFILFYLLGSAVSIKIMGKMDVLTIMSDRD"
     gene            16306..16980
                     /locus_tag="PLQ68_01335"
     CDS             16306..16980
                     /locus_tag="PLQ68_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008977133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXK71320.1"
                     /translation="MRILELKNVIFSYMSKYNKTEAVKNVSYAFERGKVYAIVGKSGS
                     GKSTLLSLLAGLDLPQSGQVLFEGISTDKMDLAQYRREHAAMIYQSFRLFPLLTVTEN
                     IIYPMGLRGFKGKEAVDKAKQLALKMDLPETVLNRFPNMLSGGEQQRVAIARALSMET
                     KVLLADEPTGNLDEKNSVNIIDILVRLAHEDGYCVIIATHDLSILSKMDDVLYMNSGM
                     LRLEEF"
     gene            complement(16981..17337)
                     /locus_tag="PLQ68_01340"
     CDS             complement(16981..17337)
                     /locus_tag="PLQ68_01340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK71321.1"
                     /translation="MSEPFVALFFVIDDKILLHKCRLENAEHYAEMRNYPRSHMDVWE
                     RHYKSEYHVDFDFYPRGRVIYDMRKEEFYIYYDPCCAKQAMKVRMMLGRRTCKLKCDP
                     NYICNQCKDKERSSSY"
     gene            complement(17330..18616)
                     /locus_tag="PLQ68_01345"
     CDS             complement(17330..18616)
                     /locus_tag="PLQ68_01345"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014119568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HXK71322.1"
                     /translation="MKTCAIVGINWGDEGKGRMVDLIAEDYDVVVRYQGGSNAGHTVI
                     NEYGKFALNLIPSGIFRREVINVLGNGTVIDLEHLSMEVDKLRKAGVYISSENLKISD
                     RAMIVFPYHKELDALEEARLKDKKYGSTKRGIAPAYSDKYQKKGIMAGELLYPEYLKN
                     HLRDILEWKNLTLQNVYNSKPYTLDDAMAWLDKYATEFKSHICDTGVLLREAKKNGKS
                     ILFEAQLGALRDIDYGIYPYTSSSTPLASYGPIGSGAPDISLDYVIGVVKAYSSCVGE
                     GPFTAEWFNEEAEELRKAGNEYGAATGRPRRVGPIDILATSYGVTVQKADCIALTKMD
                     VLSYMDKIPVCTGYEVDGVELKSFPLTPVLEKAKPIFIEMDGWKTDISGIRKFEELPK
                     EAQEYVLFIEKKINCPVKYVSVGPDRDAIILRGGDE"
     gene            18853..19635
                     /locus_tag="PLQ68_01350"
     CDS             18853..19635
                     /locus_tag="PLQ68_01350"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HXK71323.1"
                     /translation="MSGASIINCKFDANLLYYRDDCNVIIPSAGKTSEMKVLYLLHGM
                     HGTCHDWLTYTTIARLAETYNVAMVCPNARNSFYADMVYGESFYTYLTTEFIQYVRRI
                     FGFSDKRENNMIAGLSMGGYGALKIAFNNPDKFCVGGSFSGVLDMANIDNLLTGDEKT
                     DNVNKTLFRNIYGEKLDIAGTGSDLIYLIEKNNTVNGYSVKDLQVYQYCGKSDFLHLF
                     NLRFKKAAQSAGLNLKYTTDDGAHEWERWSEQIELFLKEYIK"
     gene            19640..20413
                     /locus_tag="PLQ68_01355"
     CDS             19640..20413
                     /locus_tag="PLQ68_01355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK71324.1"
                     /translation="MVGFLELIIISISVGAFVFVPLIPNISLVVQKRGKGVLTGLLCF
                     LLYGILLKNIFIYLILLIPSVDMVLSDNLLYTFFIALITSALHLVVRMLINYKKPIND
                     IFAFSSGETLLQCVYIGVPLTVLNLVYSLIINFGGFEYLGIPEKLMSVVTKLINTDYT
                     NYLPYIAYPFFILALNWLSLTVFMGNPNRKKSFYVLSFIIEFIALSIYIYLMLSKLHE
                     FNAIYMSAFFLVVLIVLLINKRKKKINKNDLLKNRTSLT"
     regulatory      20446..20618
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            20702..22531
                     /locus_tag="PLQ68_01360"
     CDS             20702..22531
                     /locus_tag="PLQ68_01360"
                     /inference="COORDINATES: protein motif:HMM:NF025833.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HXK71325.1"
                     /translation="MKKSSKHICLILVLTMILAIMPTVSAQTTDYAYVRIEGYEETLV
                     EKTAVPLEVFSLDEYGISDALNEYTALHAIIAALNANGYDASDKAVIDTNNGMFVSSV
                     LGLKAEGNAGWMYVLNDESSWNSIKDQTISANDYIVLYYIEDWMACSYSTFGLSDDYE
                     TDAFGTVKLNLKHQVTDPVTWETSYENLSGASIYIDGENTDILTDENGDAYLTFTKWG
                     TFDVSAAKYEDGINTISRPSASIKVYGRGVLVKIYTDKSEYSAYMDPKGFDLKEYNVS
                     SELEFSALHAVIKALLINGFDAKDQSVNNFNKGSYISALAGIETMGNSSWMYTLNNES
                     SWYALNEQPIKEGDYLEVYLLNDWMSDTYSYFDIKNISIQGGEKIKLRLYRQVMDYST
                     WEVTTEPYADNEIMINGKRSGLVTDSKGYVTLSFNEKGVYEISNTGKQQNVSYTNAYV
                     TVQSDRTVNTDYFNDYSDVSDEYAEYMQKAIERFIISGDENLNIRADDKVTKAEFLTM
                     VIRASELEVFGRYNKKYSDVLSGNWFSGYAQTVFKYGLAEHDNGYIYPNSDITDKDAE
                     LALNRAFGKNTSLTDEMLLVFNDGLTRQEAAYLIISYMENVNR"
     gene            22531..23937
                     /locus_tag="PLQ68_01365"
     CDS             22531..23937
                     /locus_tag="PLQ68_01365"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HXK71326.1"
                     /translation="MKKAVSLILLICFMLSILPSYASDDTLNAITDIYLDKEELSVWE
                     ALGLALNGNVVPESYYDALGQDILQRDGVYRTALEYAKIAILLKQDNKNPKDFYGYDI
                     ISSIANFKDIDKSGINGVIFSIFALVDVDIENDTVWNLDKLLDLLLGYQNTDGGFPLA
                     KGWGSDNDLTAMAVSALSYYKDNNKAETALSSALGYLSSKLDSDGFMYYQNSDSSENL
                     SQMIIALSSANISLSDERFVRNGKTVYDTLLEKYMTEDGKFKHSFSATANTIATEQAY
                     LALSSMKYGYVYAYKEHIEEPVELPDEPEPVPSENEVEDTISEPEVEKVLPFPDETEV
                     SSQYYDDLVTAYELSLVSGDTSGKLRPLDYLTKAEACVLLYKAAGLETDIFIPRYSDV
                     KKADWYSKYVIGCYFNGVVLQSGNVLFQPNENIKTEDFEKSINAILGADVTYSDELYI
                     TRQKAISMIIESIKEGGK"
     gene            23939..24343
                     /locus_tag="PLQ68_01370"
     CDS             23939..24343
                     /locus_tag="PLQ68_01370"
                     /inference="COORDINATES: protein motif:HMM:NF025833.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HXK71327.1"
                     /translation="MKKIPVVLMIVLLFLVLLSSCTQKVLEDKGKITITIDATSVDKG
                     FILTDYTVSLNEYDTVFDVTFNACKDNNILMSSRGVSAARYVEGIADLYEFDYGPTSG
                     WVFYVNEESASKSSGVYELKEGDVVRWEYVTE"
     gene            24322..25233
                     /locus_tag="PLQ68_01375"
     CDS             24322..25233
                     /locus_tag="PLQ68_01375"
                     /inference="COORDINATES: protein motif:HMM:NF014420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HXK71328.1"
                     /translation="MGIRNRVISPFYISHPVTLLTYFTALLSCIFIFKNPLFTAVAFI
                     VCCILAIYYAGFDRFLKSVRWIVPIGVLVLILNIFLVHRGSTVLFYFLYNPITKEAMI
                     YGIYNMFMLMSVMTAFISFNALIDSTRFLYLFSNVLPKTSFVFDMALRYIPLFKKRAS
                     DLSAVQNVNNKTNQKNIKDKITKSGTYLKALTAWTLEEGMDTALSLKVKNYGTRKKVH
                     YARYRYTIRDIILTVIFLSFSVFIYINAFLGETDFVFYPVTDRLEIKGYTLIIFLTMT
                     VTIFFPFILEGFTAIKRGCTKIWLQKH"
     gene            25215..26804
                     /locus_tag="PLQ68_01380"
     CDS             25215..26804
                     /locus_tag="PLQ68_01380"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXK71329.1"
                     /translation="MASKALISIKNMGFTYDNYIQGKTRKKVALKGINLDIYEGEILL
                     IMGPSGCGKTTLLRNLKKEAAPYGNLSGKIESLVDSSKISIVFSNPETQLVTTSVLSD
                     LALSMENLSYDAYTQKKRMSETVSYFGIEDLLHRHPDTLSGGQKQIISLCSQLMVHPE
                     ILLLDEPVSQLDPISALEFTDTLRRLNEELGITIVMTEHRPDDVISLAHRLVYMEQAE
                     VVYQDDTRNTLKDLWLSHEVKRKDFLPVVSKCSLFLDEKRPTCITPKELKLMAKSLDL
                     PTKQIQKRHDDFINAEKVVKIRELFFAYDRERYALRNLNLDISKNEFLCIMGSNGSGK
                     STLLKIISGILKPHIGKAKAYYKQIQYMPQDLNSFFTEDTVHEQMAFSEMDESYYISL
                     TNQLNLEELLHKHPYDLSAGEQQKAVLACMLLKKPDLIILDEPTKSMDPSLKRTISGL
                     LKTSEATVVCATHDLEFAASYATRCVMLFNGEIAYSMDAKSFFKENRYYTTSVNRSFG
                     QIDSNLVLFEDIENAYGGKGE"
     gene            26797..27483
                     /locus_tag="PLQ68_01385"
     CDS             26797..27483
                     /locus_tag="PLQ68_01385"
                     /inference="COORDINATES: protein motif:HMM:NF018816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HXK71330.1"
                     /translation="MNRKIYLFIVSFILIVIAVIVSAKSMAAALILAVTGVLIIGFSM
                     FERSKVTAERMVLLSIITAVSSAGRMLFAAVPSINPSSFIVIMSAMVFGPSFGFMTGA
                     LTALVSNMILGHGPWTLWQMLLWGLMGFLAGMFSKSFEKHIWIRLAYGFIWGFIFGWG
                     MNLFYVVSGYITETGFKAFLIASAASFVFDLMHAIGNLLLILLLGNRFIKIFNRTALK
                     YGLKDPQGGS"
     gene            27602..28858
                     /locus_tag="PLQ68_01390"
     CDS             27602..28858
                     /locus_tag="PLQ68_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HXK71331.1"
                     /translation="MESIILHADLNSFYASVECLYNPAIRNLPVAVCGSVENRHGIVL
                     AKNQPAKMVGIKTGEAIWQAKLKAANLLVVPPHYDRYLRFGNLARKIYGTYSQRVEPF
                     GMDEAWIDISFPGRSISDGYEIANQIRERIKFELGITVSIGVSFNKIFAKLGSDMKKP
                     DAVTVISRENYKEKVWPLDVGELLYVGRATKRKLRNWNINTIGDLAQADALFIRNKLG
                     KCGTILKDYALGNDYSQVENTEHETIIKSIGNSTTPPKDLCSLEDIRITSFVLSESVA
                     RRLREHGLKGKTVQIYMRDCDLEGFERQGQLKDYTNISTEIAHKAISLVEANWFFNKP
                     IRSFGVRVSDLTSSKGPIQISIFKEENKRFKVMDLEYSIDTIRKRYGPYAIQRAALMQ
                     TRQFGDLSDNTEALMREVSFNTGEAR"
     gene            28855..29124
                     /locus_tag="PLQ68_01395"
     CDS             28855..29124
                     /locus_tag="PLQ68_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK71332.1"
                     /translation="MTEDKRIKEYVNVTVKFKKDGSMIPVSIEWENGKVYTVDRILDV
                     RNAASLKAGGCGMRYLCKIMGTQTYIFFEEDKWFVERKAQCVEDF"
     gene            29082..29555
                     /locus_tag="PLQ68_01400"
     CDS             29082..29555
                     /locus_tag="PLQ68_01400"
                     /inference="COORDINATES: protein motif:HMM:NF014626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006974 - cellular response to DNA damage
                     stimulus [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HXK71333.1"
                     /translation="MVCGKESAMCGRFLDLEAREIFPSDLVEVETIQGTMDKIWGVVN
                     KYSNNTMINARSETVNELAMFKYMKPCVIPAIGYFEWDKDKKKYLFTKKNQTVIHMAG
                     VYRDDRFVIITKDAYEQFAPIHHRMPYILSAVDIQIWFDEKKLFYRDEELVCKIA"
     gene            29568..30551
                     /locus_tag="PLQ68_01405"
     CDS             29568..30551
                     /locus_tag="PLQ68_01405"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing transcriptional regulator"
                     /protein_id="HXK71334.1"
                     /translation="MAKSAEQKAKILHIYKMLYERTDEEHTITINEIIQSLSSLGINA
                     ERKSIYDDIRVLRELGFDIIGVKSKNYSYYLGSREFQIPELKLVIDAVVSARFLTSKK
                     TKELIDGLCNLVSKYQKKELIQNTGIYSKAKSQNEHVYYNIDSINEAISKNARIKFSY
                     FDYDINKEKVYRGVKGKYKVSPYALIWEDDCYYLVCYYERYNITQFKVDKMESIEILR
                     EKIDMEKRERFDLSAYTKPMFNMFKGDPVKAEIIFDMSLLNVVFDRFGLDITVQPLTK
                     NKFKAFLNVSISPTFISWLFTFKDKAEVIAPQSLVDEIKNTIDKLSKTYSC"
     gene            complement(30635..31558)
                     /locus_tag="PLQ68_01410"
     CDS             complement(30635..31558)
                     /locus_tag="PLQ68_01410"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005743 - mitochondrial inner membrane
                     [Evidence IEA];
                     GO_component: GO:0035632 - mitochondrial prohibitin
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HXK71335.1"
                     /translation="MTTLIAGLIISIALLAVIIYFNNKKDSSKKIKANCIAAVLPLII
                     SIFIASIAVIPANSVGVQYSPFTGVKEETLPEGWHFKGVFDTIYIISTEVQTSNLTGI
                     TGQTKDSQYVEMTIDVKYKVSPEKAFEVFKQYRTLQNVATSLVSPTVQRSIEAISTQY
                     NIIELLGSERNNLYMGIEEELTKRFAINGIQFVAINFSDTDAGEEIEKAIQDEAIAKK
                     RVETAEQERLRVQIEAQQRVIEASADKEKASIQAEIKLIEAQAEAEANRIISESITPE
                     LLEKMEMEARLKWGWVIVNGGSVITDIREVD"
     gene            31697..32755
                     /locus_tag="PLQ68_01415"
     CDS             31697..32755
                     /locus_tag="PLQ68_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009003693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HXK71336.1"
                     /translation="MSYELGMQAINLEMPDIVPRTEYAYQLAYGLQKAVTGIDVNQDS
                     DGETKFKAVTSFEKIWDISLFWATKIGSGIFGDKRTKMGHAGFEEGYKDFIDNKHQAF
                     TDLDDIYNMDFDKTYGSINIDETADMFYNDLLERSRRHPTGVNTTGIYVTCISGMLEI
                     LGWDMLLLAAGEDAKRFGEFTIRYSKWISQYFKALAKSKVPVCMIHDDMVWSSGPFIA
                     PSWYDKYVFPYVKKNISYLKEAGKKVLFTCDGGYDQFIDRFADMGIDAFIFEPLTDLE
                     YICEKYGKTHAIIGNADTRIIMRGTKEDIENEVRRCMDAGKKCPGYMFALGNQISPGT
                     KIENLLYYNEMYEKMKKR"
     gene            32829..33917
                     /gene="gcvT"
                     /locus_tag="PLQ68_01420"
     CDS             32829..33917
                     /gene="gcvT"
                     /locus_tag="PLQ68_01420"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HXK71337.1"
                     /translation="MKTTPLTPVHESLKAKMVDFGGFYMPVQYTSIIEEHNAVRSSAG
                     IFDVSHMSEITVKGKDAAAYIYNLVTNDISTATENQIMYSPMCYEHGGCVDDLLIYKN
                     NDEDFLIIANASNTDKDYEWMLNNAYGYSVKIENVSNEYGQIALQGPTAQTLLQQITE
                     FDLDTIPFYRFRKNIDLSGICALVSRTGYTGEDGFEIYIKADHTEYLFKKLLDLGAVP
                     CGLGARDTLRFEAALPLYGHELSDKITPLNAGLKSFVKFEKTDFIGKSALAEQAQQGL
                     DVRSAGLEMIDRGIPRNGYELFNESNDKIGYVTSGSTCPSLNKNCALGLITLEYAKIG
                     NIIYVDIRGKKLKSVVVKKPFYNKKYKK"
     gene            33931..34299
                     /gene="gcvH"
                     /locus_tag="PLQ68_01425"
     CDS             33931..34299
                     /gene="gcvH"
                     /locus_tag="PLQ68_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HXK71338.1"
                     /translation="MKFTKNHEWIKKEGEYAYIGITDYAQKQLGDIVYVELAEEDLVL
                     QQGDVLCAVESVKTAAEVYMPVSGRVVEANHELEDAPEKLNENPYDAFLAKITMDDES
                     EYDELMTEEEYNKFLESEEE"
     gene            34301..35605
                     /gene="gcvPA"
                     /locus_tag="PLQ68_01430"
     CDS             34301..35605
                     /gene="gcvPA"
                     /locus_tag="PLQ68_01430"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017000245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HXK71339.1"
                     /translation="MRYIPVTDKDREQMLKKCNAGSIEQLFTDIPDKVRLKEPLKLPK
                     AKSEMELIKYMQSLSSMNSNTDDYACFLGAGAYDHYIPSVISHLISRQEFFTAYTPYQ
                     PEISQGTLTAIFEYQTMICELTGMDVSNASMYDGASALAEACVIASSASKKKQDILLV
                     EDVHPQYSEVVETYLKFRGINLKKVTVEQLYETDLSQTAGVCIQYPSFYGDIYDMEKI
                     GEYVKNSGALFIVCANPMALAILKSPSEFGADFVVGEGQPLGIPLGYGGPYLGYFACT
                     KEHMRKMPGRIVGKTVDVDGKTAYVLTLQAREQHIRREKATSNICSNQALCALSATIY
                     LSVVGKEGFKQVARRCLDNAHYLYNGLLATNKFSKVSDMPFFHEFTVKPKEDISKLND
                     YLFENGYIGGLETEKGWLLAVTEKRTKEEMDDFIKKAGEFNG"
     gene            35598..37037
                     /gene="gcvPB"
                     /locus_tag="PLQ68_01435"
     CDS             35598..37037
                     /gene="gcvPB"
                     /locus_tag="PLQ68_01435"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008637040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HXK71340.1"
                     /translation="MANLIFDLSKKGKCAFSLPESQIPTYNLDQKYLRSVDAHLPEVS
                     EVDVIRHFTNISKRNYGVDQGFYPLGSCTMKYNPKLNEDVANLDGFKKLHPYQQSSQT
                     QGALRAAYELQEYLCEITGMAAATLQPAAGAHGEMTGIMIIKAYHESRNDFNRTRMIV
                     PDSAHGTNPASAATAGYDIAQVKSNDYGLVDIDALESMMDEKTAGLMLTNPNTLGLFE
                     RDIKKIAEIVHKKGGLLYYDGANLNAIMGIARPFDMGFDVVHSNLHKTMSTPHGGGGP
                     GSGPVCVREDLAKFLPKPVIIKKDDVYELDYDRSDSIGMVRSFYGNFLVYIRALSYIL
                     TMGPDGLTRASETAVLNANYIMASLRDSFEIPFDRFCMHEFVLSGSMQKEEYGVSTLD
                     MAKRLLDYGYHPPTIYFPQIVHESMMIEPTETESKETLDEFIEVMKKIAEEAKEDADL
                     LHDAPHNTYVRRLDEVTAARNPVLKWEKE"
     gene            37108..37809
                     /locus_tag="PLQ68_01440"
     CDS             37108..37809
                     /locus_tag="PLQ68_01440"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HXK71341.1"
                     /translation="MEDVNLTQQFVYKMTEGIIGEKWPVGNTLPSFRDLAKEYGVSRS
                     VINTAIAVLENKGYVEIVTRKGAVVRDWKKEGTVTVLNDAISLGYFQPDLIDSFFSLL
                     NYAECNGVFEAASKAKEEDIFELKITIEKEAVERNAENLVNLYLKFHEHVATMSNNIA
                     YCLVFASLKEAFTKIAQIYTLDGSAKQQCFKMHKDIFQSLKDHDGQKAKWEMQELLDH
                     LRVLVTYKTCNLKEK"
     gene            38030..38233
                     /locus_tag="PLQ68_01445"
     CDS             38030..38233
                     /locus_tag="PLQ68_01445"
                     /inference="COORDINATES: protein motif:HMM:NF014643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HXK71342.1"
                     /translation="MNMPKIVPIAELRDTKHVLHMCRATKEPIIVTKNGYGELVIMSI
                     TAFTEMSEELDKLRKQVAKDNKY"
     gene            complement(38331..39401)
                     /locus_tag="PLQ68_01450"
     CDS             complement(38331..39401)
                     /locus_tag="PLQ68_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015360161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HXK71343.1"
                     /translation="MELKKNCKYDIMTVTSMGVRITPVNRQPVNVSSIYEMQATSAES
                     NVLNISSSLGLKAKVLTKFVKDSPIALFIKAELRKRNIDFEGKDVDQGGPWGYRHQFN
                     IADSGFGVRGPRVHNDRAGEVGRTLSADDFDLERLFKDEGCRVLHISGLVAALSQQSS
                     KCCLDIAKAAKKYGTAISFDLNYRASFWINREDELRDIFTRIAAISDILIGNEEDFQL
                     SLGIKGPEAGGKTVSKTDAFKDMITRTSAEFPNVQVFANTLRQVENANSHLWGAIIYA
                     DNNWYSVEPREIPVMDRIGGGDGFVGGLLYGILKGWEPQKWVQFGWASGALVTTLMTD
                     YGQPADEDVIWSIFKGNARVKR"
     gene            39553..40704
                     /locus_tag="PLQ68_01455"
     CDS             39553..40704
                     /locus_tag="PLQ68_01455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK71344.1"
                     /translation="MKFGVKNILYIVLLISIAVSLSSCTLITQVIESSQNSNNPLFSQ
                     LPEFDPYSTNQDLNNIDMSDAEYIDDIEQFYIHLETSMEKRHRSIFVRAASHDVFADL
                     SELDKYNLKGCYKTVYSGSNGTFYAIYDIEYYPGDNVYHAYETKDKSLLNEKEKEIYV
                     LAETFIAQNITEQMSDFDKELVIHDYICNALVYQKEGIDTVDSYNITGAYALITGFAN
                     CQGYTDAFYMLTNMVGLECEKVSGESQGIAHTWNVIKIEDIWTYIDVTFDDTAFDKEG
                     LIFYAYFNVPKDYMDRSHMFYDYQELKIAEGYEYFYYSYKGSAVFNSLDFNRKVYVPL
                     SQGAASIEVFVLINDVQPMLDTLKDLNLSMQLNYFIINECTLLAINTQQ"
     gene            40732..41487
                     /gene="map"
                     /locus_tag="PLQ68_01460"
     CDS             40732..41487
                     /gene="map"
                     /locus_tag="PLQ68_01460"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003446049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HXK71345.1"
                     /translation="MTNIRTKSQIDKMREASKIVAACHENLRDVIKPGITTMDIERFV
                     DRFVASHNAICAQKGYHGYPFCTCTSVNDEICHGMPSSYILKEGDIVKVDMVVNKDGW
                     MGDSAWCYAVGQISEEANKLMTVTRQCLEIGISQAVCQNRLGDIGYHIQSHAEKNGFS
                     VVRDFTGHGIGDSIHEDPAVLHYGQKGRGIRIQEGMVFTIEPMINQGDYDLYIDEDNE
                     WTAYTADGSLSAQYEHTIAITANGPEILTLQEF"
     gene            41489..42847
                     /locus_tag="PLQ68_01465"
     CDS             41489..42847
                     /locus_tag="PLQ68_01465"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXK71346.1"
                     /translation="MLQKIKTGSQKIYIALFYRNDTKLLTLPGKKDRDWFIIQALIGT
                     VFALFTGATYLTGLFNKVNAPEILIGYLPIIGSIAGVVTIFAGYTMQRIRSRKKFILI
                     CNTILKTLIVLIAWIPLIFPNQNAAYIMIIMALIAYALNNFMAIAINSWFVDLIDSKI
                     RGQYMSVRNMVALIANLIVPVIVSAFIDTSSDQYLAIVVVFTIAWIIMWAESYSFYKI
                     SEPPMSPATKKIKLKDVFIVPIKNKDFMKFMVPIAAFYFIWYIAMSYGTLYQLNYLGL
                     SYTFISLASVLNVILQFIWYPIIGKLVDRYNTDLLLMACFMLYFIDSLIWGFTNLSSK
                     YLMIILVNVIASMTAPFFSLTLFKKKYDIIPQTERSLYDGFYTAIIASIIALAPIIGS
                     LLKDYIAVNIKPFWIFEVPQFQLIFMVTNILLLVLILFNLKKTIRLYKEAKSQGPVDT
                     DN"
     gene            42862..43857
                     /locus_tag="PLQ68_01470"
     CDS             42862..43857
                     /locus_tag="PLQ68_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017415538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family peptidase"
                     /protein_id="HXK71347.1"
                     /translation="MEILKKLTDAFGVSGREDEINAVITGLVKDYADEIKYDGIDNVI
                     AVKKGNGKDKKKIMVSAHKDEIGFMIMSIDSDGYINLRPIGGIPVSLSIGNRIIFKNG
                     TVGVLSFKKDSENIKNNNVGDMFADIGAKNKEDALNYVKPGDVGSYYGPLCELKNDRV
                     IAKALDNRIGCYIAIKTMMENTKPYNDIYYVFTSQEELGLKGATVASRVVKPNIAVAV
                     DVTSSFDTPVTKEGNSVLGGGAAIKIMDRSVICDEKLISIMQELCKKSEIAYQSDILA
                     AGGTDAGAIYTSNEGVRTGGISIPMRYVHGPVGMVDMNDVKACISLLKGFIGQKI"
     gene            43988..44926
                     /locus_tag="PLQ68_01475"
     CDS             43988..44926
                     /locus_tag="PLQ68_01475"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HXK71348.1"
                     /translation="MDENSLRIYKKNEKKIFTKVNIMIYLMIVPGVVLYYVFRYITMP
                     GIMIAFQEFTTGGFKYWCGFENFDKLFGLRAFWQAFRNTWVFIGLEYVFIKPSPIILA
                     LLLNEVRIMWFKKTVQTVSTLPNFLTWVVVSGIFIQLLSPSTGYVNAIIALLGGERIY
                     FLGRAQIFPWLLTFIRIWRGVGYSSIIYLAALGGVDPELYEAAVIDGASRWKQTLYVT
                     LPGIKNTIVVLLVFSFSGIMGGLFEPVFTLTDGNALLDETAMVLDVYIYNTGIRQGKF
                     SMSTTTGLFNSIISTILMLSANYASKYLTEDGRTII"
     gene            44943..45839
                     /locus_tag="PLQ68_01480"
     CDS             44943..45839
                     /locus_tag="PLQ68_01480"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HXK71349.1"
                     /translation="MLKRSLGEKVFNIFNLLGMLVFSVIIIVPVLFVFRNSFELSETS
                     TTTLRLWPEQFSMVFYGMVLKDESVYRPLLNSVFVTVVGTPLMLIINALGAYTMSKRE
                     LPGKSFFAYYMVIIPMLIGGGTIPGFLLIRSLGMIDTLWAIIVPGLASGWNMTLIRNY
                     YWSIPNELTESARIDGAPEMTIFVKIILPLSKPVLSALGLLTGIGLWNTFYAAMIYIR
                     SPIKWTFPVKIRETVMKNEGNDKLWEEMMVAAGLDPTELLINQSGLGGAMMVTSMLPI
                     LIVYPFLQKYFAGGIMVGSVKG"
     gene            45990..47039
                     /locus_tag="PLQ68_01485"
     CDS             45990..47039
                     /locus_tag="PLQ68_01485"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HXK71350.1"
                     /translation="MDYRRTDEIIKRTKAVYEAKKGTLIQVKDVSGIQVPKRALDSFG
                     LPDNMETYLDYVIDKDKAYWSVREQIQDDIIPSVTARYGIAEHSAFMGGDVSFTENTS
                     WHHKHVEDYSNYTLSKVDESNIWRNLVIEGMRYLKEKVKDEFFVRYRGADGPLDIVNA
                     LRGNDIFYDFYDEEEGLIELADKCTNAIIYMLKQQQKIVTEYKGYVISGFDVIMPKGY
                     AGHLSVDATTMLSDEMFRKFEIPFLNRIADNFDGLFFHIHSVGSHVIPAVCEIDKIKF
                     LEISNDPNSPRSIEVYRKYEKELSEKKVIVTLNYDEIKDNVEFLKDRKTIIWYDAQTV
                     DDALRAVDLVRNKLG"
     gene            47215..48819
                     /locus_tag="PLQ68_01490"
     CDS             47215..48819
                     /locus_tag="PLQ68_01490"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HXK71351.1"
                     /translation="MYTKYIKTFDLTAESIARSDILMNYVNNDYYYTDVLHDNSDMFF
                     YLDLISESDEYIDHIMVYDYSFEYLITNKNYYAAYMDVSMFPWFVKNVSQIKPISELD
                     NYCTWSLYKEYVAITYRADMVNKSLTRRTFVTILINNEKFNRLLSGLALTDNSKNAFA
                     VKNDIMLYFDGESDLTSTIKHLVFENFKDLNEGERVMFIHDYQWYYLQYRPLGTSYEA
                     GIVYLIPDSDLITNEYEFMIISSLVISLIVLLLGYVIFRLVLNSNIQKPINQLIKGMD
                     KVGNGDFTYKLDESICKDNDMRGLFAAYNSMTDRVTVLIEELYQAELYRKQLELQALQ
                     DKIDPHFLYNTLDTINWTAKEHNVDEISRIVIALSTMYRKIFNRGKDLTTIKDALEGI
                     ECYLEIQRIRYGESFSYDIYCPEELEKCIILNLIIQTIVENSIVHGLEDRSSGKLEIR
                     VDKEKDDIIIKVIDNGIGMTEEKLNLINSSINSMKLESESGLRNVQKRIRLYYGIQYG
                     ITIESKYKEGTTVILHVPYREKEEDD"
     gene            48812..50431
                     /locus_tag="PLQ68_01495"
     CDS             48812..50431
                     /locus_tag="PLQ68_01495"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXK71352.1"
                     /translation="MIDIIIVDDEKSIREGIAVSIPWEKYDINICGVASGVSEALDLI
                     EKYMPNIVISDISMPEMNGLDLLEIVNRTYPNIKVILISGYRDFEYAQRAVTNNAFCY
                     LTKPLVAETLISKVLEAKQEIDAKLKEVKLNDNIRRKLRENIMVLKDNFFRMLMEGRI
                     RQKTEIMERATTTELNMDYSNFIVCVAEFELAASQKKLNMYDFSFYKAAIMSTIEEHL
                     EDIYKCYTFNLDNRIGILVCSNQQIQRNILINKLNNVKSWVNKNMGFSLSAGVGNTCG
                     SVEKLTLSYISAVDSMLYRVILGKNIIVDAEQKISASKERMAVDDFDNILRNSEEDMV
                     FALRNLNSTEIKNIIDQIMQASKQIVSNDVKQKERIAFLLSVFIIRVMYSLEMSNHRF
                     YNKENDLYNSLNELISIDDMKNFLMGIIKEMIQTIEELDSKQNNFLVNKGLRYIKEGV
                     YEDVSLVTIAERLQIHPNYLSKIFKQVTEESFTEHVIRYKMNEAKKLLKTTNAKVYEI
                     SDKLGYKDVAHFTKLFKKHFGISPTEYRQLL"
     gene            50456..51274
                     /locus_tag="PLQ68_01500"
     CDS             50456..51274
                     /locus_tag="PLQ68_01500"
                     /inference="COORDINATES: protein motif:HMM:NF016816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HXK71353.1"
                     /translation="MKKYMIRCDMEGISGIVSYEQADPKKSEYQYGKKLFMSDLIAAV
                     SGLIAGGADEVHIYDEHFFGRNIDLSEISSISSSDKIFVYMGKPPYEEDWAGGLTSDF
                     DGLVLLGFHSKKGTKNALLNHTYENDIEDILVNGISVGEIGIETMIAKQMSVPLIMIT
                     ADSEGVKEALQLEPEVIAVSVKESLGENSAICYPSNRTSQVIYDKAKEAAQREWNRPV
                     EDYKTGVHMKIRLADTIYSKEYLKMFSFPEFREKTVLCCWAKYWQNKLKVMEKL"
     gene            51271..52176
                     /locus_tag="PLQ68_01505"
     CDS             51271..52176
                     /locus_tag="PLQ68_01505"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HXK71354.1"
                     /translation="MKEDITQYAKLGLVHHMLFPKSIEDETFHADSLIELSKRNDIET
                     FDCCLPYDKSNRNRVIEQLSNCSKEVCYALHLFPSRKISLCSLDKIEQSITRLVVADQ
                     IEAASAINAKGFVFVSGSDVPKNRDCAKTAFLDFTKWFANELRKKGITALLEPFDRTV
                     DKKYLYGPIDECISLIDEVSLHYDNIGIELDMAHLPLMGEDFESSIRRCGHRIKRVHL
                     GNCVLNDANSTWYGDKHPPICFENGMIAESELRIILETLLDIEYLSKANRGSLVMEMQ
                     PYPNKSAEYTIKRTMEILNKVWEQI"
     gene            52401..53234
                     /locus_tag="PLQ68_01510"
     CDS             52401..53234
                     /locus_tag="PLQ68_01510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK71355.1"
                     /translation="MNFFSNSLVYFIKKPLIPIYVASVTLVYCIIAIFNPLKVLTKSY
                     ASFITEDFGSTIVLLSKEIYSYTNIPYIVLGILGIAIVLALLSSLLFSGYMNIIHLTV
                     KRIKTDFSDYLQGIKKGFFRCALVFFQLYISILLFVGFLPLALTPFFILRDSVLESGN
                     DMTILYILLGVIIFICITIVLLIYMSFMFKFPSVFHFSKYPIEKSNAAVNAMYWRTFG
                     KVTLLLTFLAGIVFVMFKIENRVLEFLIGFILYTVYFSFFVVFPFYTFDKLIEPYRIS
                     N"
     gene            53271..54164
                     /locus_tag="PLQ68_01515"
     CDS             53271..54164
                     /locus_tag="PLQ68_01515"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HXK71356.1"
                     /translation="MYDVVIIGKGPAGAQAGIYLGRAGFKTLIVYKDGGSLSKTDFIE
                     NYYGYAEAISAEKLLNEGVLQAKRFNVDFVEDEIVMLQTDEENKVLVKGLKKDYTASY
                     AIIAAGMTRKSLNVPGLKDFEGKGISYCVTCDGFFFRNRKVAIIGYTKFAEHELNDLL
                     MFTKQAFLITNGKELEFDASVYKEVTVIDKPIASIYGEDTIKGISFKDGSNEDFDGIF
                     VAYGTADASSFCVKSGIATEKSRIIVDENLMTNIDRIYAAGDCIGSYMQVAAAVADGA
                     IAARSVTERLKADRKTARKKD"
     gene            54222..56150
                     /gene="metG"
                     /locus_tag="PLQ68_01520"
     CDS             54222..56150
                     /gene="metG"
                     /locus_tag="PLQ68_01520"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008691220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HXK71357.1"
                     /translation="MNKEKFYITTAIAYASGKPHIGNTYEIILADSIARFMRMQGYDV
                     FFMTGTDEHGQKIEDKAKQSNMSAKQFVDKSAKEIKRIWDLMNTSYDKFIRTTDADHV
                     VQIQKIFKKLYDKGDIYKSEYEGWYCTPCESFWTESQIEDGKCPDCGRDVKKAKEEAY
                     FFKLSKYQDKLMEYIEKNPDFIQPESRKNEMINNFLKPGLQDLCVSRTSFEWGIKVPF
                     DPNHIVYVWIDALSNYITGVGYDADGNSSEIYKKYWPCDLHVIGKDIVRFHTIYWPIM
                     LMALDIPLPKKVLGHPWLLFGEGSKMSKSKGNVLYADDLAELFGVDEVRYYVLSEMNY
                     ANDGIISYKQIIDKVNADLANVLGNLVNRTITMQHKYFNGIVKKPESLLPQNQFDEEM
                     IELAKQTLNKVTELMKQYKVSDAIYEVMALAKRCNKYIDETLPWTLFKNPDDHKRLEY
                     VMYNLLEGIRTVAVLLYPFIPSTAEKILAQLNTKKTDINSLEEYGQLEDQIMLNQPEI
                     LFNRIDSDKKLKEIEESVKEDQEDANVVQIGIEDFAKVELKTAKIIKAEPIKGAKKLL
                     KLQVDLNGKTRQIVSGIAQYYECEDLVGKTVIVITNLKPAMLRGELSEGMLLAASNSD
                     KLSLVTIDKEMPSGCGVS"
BASE COUNT        19530 a         8300 c        10965 g        17377 t
ORIGIN      
        1 tgatcttgca gcttcattag tagaagctct agcacttgaa acaacagaag gaacaaaact
       61 aatcgacgct atgatagctg acggtattgt taccgaagaa gaagctctcg aagcagaagt
      121 taaagatgat gagcctgtag cagatgcagt tacagtagct gttactggtg ttaaaaccat
      181 taaggttaca tttgcagaag ctcaggatgc agatacttca gtatttgtac tcaaaaaggg
      241 cgctgttgga caagcactta aaacaccttc atgggatgca gcaaagaaag ttgctacaat
      301 cgaaaaagct attgctttta caaaaggtga ctatactatt acagctaacg gcgtagcagt
      361 tgactttaca gtagaagcaa acgaaacagc tacagagctt gttgcttcag catcaacagt
      421 ttatgcaaaa gctggtgctc aggacattaa agttaacctt cttaaccaat atggtgagaa
      481 gatggtattt ggtgttggca atctggtata tggtgcttct ttaggaacat tagcagaaaa
      541 agacaacaat acattaacat tgactttacc tgcaactctt ccaaaggctg gatcaacatc
      601 aacaatattt gcatactata caccaacagg attttcaatt aatactacta ttacagtatt
      661 agaagatcaa gttcttacat ctatagtatt taatggacct ataactgtag gtgatgaatg
      721 caaagataat tataattcca gattaacaga aaaaacagca gggaataagc tttcttataa
      781 agcatatgac cagtatggta atgctttaag tcttgcaaca ggcgaaggca caacatatca
      841 gttagtagtt agtggtggaa gtgttgctag caaagatggc aaaactatga ctcttacagg
      901 tcttaaaaaa ggcacattca gtatcagagc tatagctctt acaacaggta atatttctga
      961 tttgtataca gaaactgttt atgctaaacc tgcacttgca tcatatgata taacattgcc
     1021 agaagcaatt tatcagaatg aagcagcaaa attcacaatc gtagcagttg accaatatgg
     1081 gaaagcattc aatgttactc cggatgcaac aacatggtca gtaacaccac tttcaacaac
     1141 aattacagca gcacacatga ctgttccagc atatgctaag aatgaaatta gctatacaat
     1201 tactggaaaa ggaaatgcag acttgtactt cacaactggt tccttgacag ttacaaaatc
     1261 tgtagttgtt caacctgaaa aagttatatc ctcaatagca tcagttccgg tttcaaccga
     1321 agcattgctt atgggtaaaa caatcgcatt tgatctttct aaggtagttc ttaatgacca
     1381 gtatggcaat aagattgctt atgacgcatc agtatggagt tatgacctta gaagacttgc
     1441 tgatacagct cctcttggaa atactgcaac agatacaaat ggtacagctt tagtacttac
     1501 tgcaccagta ggcacagcta catcatatgc aatcaaagca catcttgaag gaacagatca
     1561 gcttcagtta acaatctata agggcggagc ttatggatca acagatacaa acaactatgt
     1621 atcatataca tatgatttct cactcgcttc agtgaaagca gcttcagtag tagcttatga
     1681 acttagcgtt aaagaagaaa tgtatgctgg aaaaactaca acagaaatgt ccgctacaca
     1741 tgacttggca gttgctttaa ttggtaagac agctaatggt actacagtaa cattagtaga
     1801 ttcttcatca acagcattgc catctgatac aattagcaag tacacaacaa caggtgctaa
     1861 tgttactctt gcaacagcaa caagcaaact tcaggtaaca acagctgtaa caggtactgc
     1921 tgatggaaca acaactatca gagcatggaa caacaagggc gaatcagttg cagaagcagc
     1981 tgtagcactt agaagagctg cacctaaggt tgacacactt acattgactt acaagtctgg
     2041 tgttgcaaca ttagcagcaa aagatcagta tggtataacc attactcctc ctactggaaa
     2101 attctttagt tcaaattctg atttgactat tggagcagta acagtagcaa cagacaagac
     2161 aacagtcgtt atcaacggag cagcaggcaa gacagcagtt atccgtttcg tatctgacga
     2221 tggtgtttgg gctcgtgaag tatcagttac taagtaattg attctcgaaa ttagaattaa
     2281 gagggcagag caatctgctc tctttttttg tatcaatttc gttacatttt attattcttt
     2341 atattatctg ttataatggc tatataatga aaagaacact tagcattatc ttaataacag
     2401 tcattttgct aagctattgc gctatacagc ccaaagctta tacatctgta gaaatcgcat
     2461 atggtttgaa cctgcttaga ggtacaggag aagggttgga tgaggcatat ctttctatga
     2521 ccccgatgag aacaaatgca gcaatgatta ttcttagatt attaggattg gaaaatgatg
     2581 ctttagaaca tgagaaaata tcaacttttt ccgatgcatc ttctgctact gcttattggc
     2641 aaccaatatt agcatatctt tatgataatc cagatgtagg tttttcaggt tatggagatg
     2701 atactttcag acctaacgag agtattaatt ctcagatgtt agctaaagta ctattaactg
     2761 ttttaggtta taagcaggat gttgattttt cgtgggcaga aacattaaaa tttgctgatt
     2821 caataggttt aaaatctttg gttaataaaa gcactattac aaataatgat gtagcaattg
     2881 ctttagttga agctttgagt ataaaaacta aagggggata cacatttgta actgaactgg
     2941 tagttgcgga tattattaat atagatcagg cagaaaaata tggttttact gttgaaacca
     3001 ctcaagtgga aataaagaat gcaaaggcaa ctggagttaa gacaataaca gttgaatttc
     3061 taatgcctat acctaaaaat tcaacagtca tgttaagaaa agattttatc ggtatcagta
     3121 atacatatac cttatctgaa aacagaagga tactgacaat caatactact gctacaatga
     3181 cacctggaca atacacagta gctgtagctg gttcagtagc aacttttgag gttttcaatg
     3241 aaaaagcatc atccttagtt atatcaacag aaagaattta taaggatgta ctgcagaata
     3301 tagggctaaa actcttaaat cagtatggag aggaaatgcc gataacaaat gtaaatatat
     3361 ctgtgacaaa caaaacttca gggtcaagaa aaattactgt tacgaaaata gataatgagg
     3421 tatatcttga tgtatcatat gctaatttag cagatcagat ttatatttat gccctagata
     3481 attccacttt acttaatgat tcagcattac ttacagttca atctgtacct tcaataaaga
     3541 agataagtat attaggcgta gaagcttcat cagatacgaa taggatatat gaagatacat
     3601 cttcacatat aattaaaatt caggcatatg accagtatgg ccagaaatat tttattagac
     3661 agtcagatat tgataaaaaa aacctattac tgatatcttc aaataatttg tgtataagtc
     3721 cgtcaaatat aaaagcggat ataaatggga atcttgtttt cagcactaat atggctggag
     3781 aagtattaat aaatataata gtatcagcag aaggtatatc tgcatctact acaatagcag
     3841 tatatgcagc cccagtatta agcagtataa aagttgatca gctcccttcc actattttta
     3901 aaaacgagag agtggaagtt ggtgttatag ggtatgatca gtacggcagt atatatgaaa
     3961 taaatgcgac cgacatcatt gggtatacaa ctactgatcc tatgattgtt cctgcagcaa
     4021 gtattagtgt taaaagaggg gttttgtctt tcaatacgtt aaacagcggt gaagtgtctg
     4081 taaattccag gataaataat tcattgatgc ctttgtttac tattaaggtt aacgatggta
     4141 atgtaccata tagtataact agtcttgata taccgttttc acattttgaa aaaggaattg
     4201 agaatattca gattccttta tcaaacataa aagttgtgga tcagtatggt aatcctaatt
     4261 cattaaatga aagacctgtc tgggatactg ttgaatgggg tatttatatt gataaagtgt
     4321 ctggaaatag ttttttatat gaaaacggtg tattttcaac tactaataca gttggaactg
     4381 atgtatttaa tgtgtacata accagagatg gaagaataat ggatagatca ggttatagct
     4441 ttatgttaac taatgtttcc gcatcagaaa ttcaggtatt cgaaattgat attccaaaga
     4501 taatatatgg tggagctaat aacaggatag aagcacatac gaaatatatt acaattaaag
     4561 ggtatacatt aagtaaaaac cctgtaatgc tgaaaaccga tgatttaggg atgcctacac
     4621 tgatatcagc tattactgta tcaaatagca aagtttatat agatacatca acttgggcaa
     4681 taaaatttaa cacatatttt gaaaatgatg atattgtgac agtaaaattc tggaaagatg
     4741 gaatggaaat tgaatcagaa gatatagtta ttatggctac cgaatcgaaa gtaagcagaa
     4801 tggaatatga ccaagctcag tcatttatca tatcttcttc tgaatactat actcttcctc
     4861 taaatatata tgatcagtat ggcatagaaa ccaatctacc agaaaatacg cagtgggtta
     4921 ctaacagtaa cttgatagac agcataacat tcgatccgtc gactaaaagg cttagaatag
     4981 ttataaaggg ttctgtgata agtgagacag aagtatatat ctcatatatc gctccagacg
     5041 gatcattttc cttcagaggc ccctatacta taatgcctgg aaggtactga ttgaaaatta
     5101 tgtaaaattt atatatcatt attaggggtt attaacgatg ggacataaaa tacttgttgt
     5161 agatgatgaa aagaatattg ttgatatttt gaagtataac ctggagaaag agggttatga
     5221 tgttatttgc gcttatgacg gcaaagacgc catatttcaa gccttaaagc ataagcctga
     5281 cctgatactg cttgatatca tgcttcctga atatgacggt ttcatagtgt gcaggaagct
     5341 tcgtgaaagc atgactgtac cgattattat gctgtcagcc aaaggcgagg aattagataa
     5401 ggtaatagga ttagaaatgg gagctgatga ttatataaca aagccatttt ctgcaagaga
     5461 acttattgcc cgggtaaaga gcaatttaag aagagtaaca tatgcaaata atgataattc
     5521 tgaaattaca tatctgaagc tacgtgattt agaagttgat gaagctaaat gtgaagtatt
     5581 gaaggaaggt aaagttctgg accttacagc aagggaattc gaacttcttg ttttctttat
     5641 gcgaaatcct gaaaaggtat tcagtaggga attcttgctt gataagattt gggattatga
     5701 gtattacggc gagatgagga cagtggacgt aactgtaagc cgtttacgtg aaaaactttc
     5761 tgacgacccc aatgaatata ttgtaacaaa gagaggaatg ggctattact ttaaaaaaga
     5821 ctgataagca tatgtcatta cagacgaaaa tggtcttcta tataagtatt atcgcaatat
     5881 gtatgataat acctataggt gtgcttatga ataacaatgt tgagcagtat cattacaacc
     5941 agtttgtaag aaatattgaa aacggagtta atatgctcag ttacaatgaa aatgaggaac
     6001 ttgatattta tactgtatat acagatatgc gtgatctgta tgctccatca ttcggaatat
     6061 atggaagtaa caagagttat acaatagtag acagatactc aaatgaaata tacagtaatg
     6121 actctgtgtt tttatcttca actgattctt cgtttataaa tgaactgttt ttatctgcaa
     6181 attttatgtc ttcaatggct ggaagcccta ttaatagcaa cgcgactttg aatttaaggg
     6241 gaaggacttt ttatgattac gccattacaa taagtaatat tgtggtttat ttcagatact
     6301 acaaagcaga ctggcaggat atggttacag agtttaatga aactttatta tctgcacttc
     6361 tatttgcaat aattattgca ttcattgtag gatatcttct gtcaacggcc ataactaaac
     6421 ctataaaaga catgactata aggaccaaag ccatagctgc aggtgatttc ggtaagactt
     6481 tagatattaa agcagatgat gagataggac aactgtcttc aagtattaat aatatgagtt
     6541 acagtctgca aaatatgtta gatgagatat cttctgaaaa gaataagatg gagataatcc
     6601 tggataatat tgaaggcgga gtaatagctt tcaacctaaa aggagaaatg attcataata
     6661 accctgcagc atatattatg cttaacagag ctgatcttga tttcactttt gaaaactttg
     6721 taaaagaata taatctggat attgatgtat cagatattcc tgcagattta tataaagaat
     6781 caatgcttaa tattgatgat gagacattga ccatcagtat cgcttctttt aaagatagtt
     6841 tagggaatgc cgagggtgta attatcttgt taagaaatat aacaaaacag tttaaactgg
     6901 atcagatgag aaaagaattc gtagccaatg tatcacatga gcttaaaact cctctgacat
     6961 caattaaaag ttttactgaa actttaatgg caggtgtaga cgatgaagaa actagaaatc
     7021 attttttaga agtcataaac tcagaatctg acaggatgaa taatcttgta caggatttat
     7081 tgcaattgtc ttccatggat atgggacgta tggtattgaa gatatcccac cataatgtaa
     7141 gttcacttat tacagatgct atgcgtgctg tagaggcaga agcaaatcat aagaatcata
     7201 ctcttgtttc ggatatacag tataaaggta ttgcatattt tgattatgac agattgctgc
     7261 agtgtacaat caatcttctt gtcaatgcaa ttaagtacac agatgaatat ggaaaaatcg
     7321 agatacttac ctatgagcag gaaggttact tattcatatc tgtaagagat aacggaatag
     7381 gcatatctaa aaaggaccag gaaagaatat tcgaaagatt ttacagggta gataaggcaa
     7441 gaacacgaat aatgggagga accggccttg gtttagcaat ttccagcgag atagttaaag
     7501 ctcataacgg gcagattgaa gtgaattcca aagttaatca gggtactact ataacaataa
     7561 agataccttt aacacagaag gaggatgagc aatgaaaaga aaaatagctt atccgatact
     7621 tatcatattg cttttagcaa cggtattcca gtccttcatg ctcataaact atcattctac
     7681 aggcagactc ttgccaagaa ggaccataga agggcctaat actgttacgg aagtaattaa
     7741 ttttgaagaa tatcttttag agtcatatat aaggccattt gagattacaa tgactacagg
     7801 tgaaggactc aatgttaaca aggtacccag agatttacaa gggtactttg accagatatg
     7861 gaaagatacc ataagtgctc ttgcaacact tgccgataaa aagctttatg agatatatga
     7921 tgaagacaag tggaatgaaa taaccggttc agcaggatat acaattaaat tgggttacga
     7981 ttgccctata aggttcataa actgggttac aggttctgta aattcaaaca gtgaaataga
     8041 cagtataaat aagattatgg taatacccat agattctgac gaaggtgatg tttatattag
     8101 aagcggtaat aaagttttca gatatttaaa tgtcaaactt tcagggcttc tcaaacagaa
     8161 tgagttttta agtctgtatg atgaaataaa gcagcaaagc gaaataactt atgtatacct
     8221 taatgaagta gctctttatg agaattttaa aggaaatatc gaaccggatg ctactgtaat
     8281 aataatggat gattatgtaa ccattaacag gataagagtt gcatcatatg agatattgtc
     8341 tgagtgtatg tttaatcttc agattgagcc tgatgttaat aatctgtcac ctcttgtaaa
     8401 gcagtacata agtgaaataa agactaaatt attcggtatt tattctgatg tttacaaaga
     8461 agttattgca cagaacagag aacttacatt ctctgaccag tataatagtt acagcataca
     8521 tacagacggc tcgttatctt acagatatgc ttccgcctca tatattcagg aaaaaggtga
     8581 tatatcttta gcattttcaa atgctttaag catgcttact gatatgactt cattatctgg
     8641 gactaaacaa aataatcttg tactaactaa agtgaaagaa caggatgact cttatgcatt
     8701 cagctttaca tatttcttca acgattatcc agtattgatg cagaatgaaa agtatagtgt
     8761 ggttataaaa gctactgctg acagagttat atcaatggat gcgaatctgt atgatataac
     8821 tgacgcatct gtcgcaggaa gttatatgga tttatatgac cttagaacat ttaatatgct
     8881 ttctgaacaa agcatttcat tagggtcatt aaaggcagat aatatgtata taggatacat
     8941 aaaagatgaa gatactgttt tattgccatc atggataatt gatgatgaat ctgttttagt
     9001 aatagaactt aaggaggcag aagactaatg gattctaaaa aagctctcag aattattatc
     9061 atactgcttc tcgtagtcaa tatattcatg actatatata ttaccaacct tgcattctct
     9121 gaaccgaaat ctgaagatga gttgaaacat gttatacagt tattggaata cagagatatc
     9181 aaactggagt gtgatatacc tcaatatgct gctccaagcg ctgagttaat atatatagaa
     9241 gcggataaca ctgaaattct aaattatctg agcaatatag aaggtttact ttatgaagat
     9301 gaagcttcta aacagattat atttgagcca gttgtaacgc agagatatga aaatttttca
     9361 ctggacttag ctacgcagct gtctgattca tttgttgaaa atcttcctgt aaaaagcgaa
     9421 tcatatatgt tagattccat acttactgca ggagtaaaca tatataagtt cagctatatc
     9481 tacgttgatg ggtccagtta catatatgat aagaaaatag agattacaat aagcagagat
     9541 gggattgacc aggtaataat cagtaatgtg aattatcagg tttctgatac cgaatataac
     9601 agggctaccg tatcaatagg aaatatttta gcaagcggat ttatccgaca gggccagtct
     9661 tttatcacaa taaagaagat agatgcaggt tatttatatg atgatgagac ttcttcaata
     9721 atgaatgtat ggagagttat gtataataac ggacaagagc ggtatttttc atcagttgac
     9781 ggaacaatta ttaataagaa ctaataacca aacctttata tagtttgata tctatttata
     9841 cagtatccta aatctattaa aatatctcat gcaaactata ataaggaaat ggtacaataa
     9901 caattgaata caattacatt actaggaggt agtatatgaa gaaagaggat gagtttagtt
     9961 tagatgaatt tgatttagag aattttgaaa tagaagattt taaatttgat aaaagcgaat
    10021 atgataagca atgcaaacaa cagcagctta ctaattctga atacctggaa atttttgaaa
    10081 atgatatgaa gaaagctaat cttgcaaaga gtacaataag aagacatcgg agcaatgttg
    10141 atttttatat caatacatat ctgcttagag aagactcttt gaagatgagt gatggtactt
    10201 tcatgataga tatgttttta ggctattttt ttattcgtaa atgtatgtgg tcaacgcctg
    10261 gaactataaa gaccactgca gctagcataa agaaattcta caaatgtatg gcagaacatg
    10321 gatacattga aaataacagt tataattatt tatgtaatga aattaaagaa aacatggaat
    10381 cctggcaacg tgagtgtaat gaatataata atggtagcga gtatttttac atagacgagt
    10441 aaaaagaatc aggagtaatg gtaaaatgac tgagaaacaa gcaaaaagaa tttttaaaaa
    10501 attcaacaat gcgtcagatg tgattcgctg tccaaacggg aaagccaata caaagaagct
    10561 tcttcattct tatgcacgtg cagctgtaaa cctatatggc attataagct gtaatgaact
    10621 tcttgatatt tttaacgaac aaaataacga gcatatagaa tacaaggata tttatacttt
    10681 tcttctacca cttgtgctta aagaaggctg gtactgtttc tataagcatt acattgttca
    10741 ttattcattt tttactgatt ttgaacaagc agattacttg ctggaaatac aatctgaaaa
    10801 accgcggtat atccctgata gaagcacttt tcttcagtat gcagatgaat attatacaga
    10861 taatgataac ctattgaaca tttaccttta catcagtgat cattttggta gaacaaaaca
    10921 catatatcag atattcaatg agattgaaga atatataatg tatagtaatg atattggagg
    10981 tttgacagat atactgcaaa aaattgactt tatggttgat gatgaaaaac agatacagga
    11041 tttcataaat atgattacag atgccaaaaa caacacacgt atttgggaaa ataaaggata
    11101 tactccaata gaactgtgcg atattatgaa agaacaaaac aaaaaaatta ttaagtttcc
    11161 agtatatgag aagagaaaaa tcgagcgaaa tgaattatgt ccatgcggaa gcggtaagaa
    11221 atataaaaat tgctgttctg tttttgaaga taaaaaggaa gcccagttga gttcagaaga
    11281 atgcagagaa ttttatgaga tgtggtatgg gttattaagt tttgtcaatg ataagaaaaa
    11341 tattcttaat atcagaatta caactgatta tccaaacaat ataaacgatg ttaaattaca
    11401 taaagtacgc gaagtattat ggagcaatcc tgaactgata gaggaatata taaaaaaggt
    11461 ggctctaacc aaagaaaaga ttgatattct aaaattatgg aaaactaatc atataaaagg
    11521 tatgtttttt gttctggagt ataaaccaga atatgcagta gcaattactt cgaatgaaaa
    11581 cggtgaagac aggctttatg gaataaaagg tataagcaat ccaatatcga atatattaca
    11641 aagaaattta ccaatacaaa ttgaaactgt attgcttccc ttcaaaggta agattattta
    11701 tgatacctat ataggaactt ttcaaataaa atatttagaa ggtgtgaaaa agtttttcaa
    11761 agagatatat gataaagcaa tagaatacgg aattattaca tgtttagaat aaataaaata
    11821 aattaaagca acatcagatc tgcaaagtga agatgatatc aattataaaa ctaagaagtc
    11881 ataaaaaaat gtaagaacca caaaaagtat ataaagagtg tataatatat ataaaagtaa
    11941 ggagatttag tattatggat ctatattcaa atttacaaaa cagattaaat gcaatgaaac
    12001 aaggcggaac atacaaagag tatcagtatc ttacaactcc aatgagcagc aaatcaaaca
    12061 ttgaaaagta taacgacgtg attgttcttt gctcaaataa ctatataggg ctttgtgata
    12121 ataaagagat tatacaagca ggtcatgatg ctttggataa atacggagtc ggagctgctt
    12181 cagttcgttt catttgtgga acttatgata tacacagaca gcttgaacaa aaaacagctg
    12241 aatttatgca tacagagagt tgtcttacgt atatgacctg ttggaatgct aatactggtg
    12301 taattccagc cattaccttt gaaggtgact gcataatatc tgacgcttta aatcatgcta
    12361 gcattataga cggatgtcgt cttgcaagca aaaaagttga aagattcgta tataagcatt
    12421 cagatatgga agaccttgaa aggttattaa aagaaagtga cagatttaat accaagcttg
    12481 tcataactga cggagtattc tctatggaag gtgatatagc caaactggat gaaatatatg
    12541 tattgtgtaa aaaatatgga gcaatattaa tggttgatga ttctcatggc atgggagtta
    12601 ttggcgacac tggaagagga gctattgaat atcacaatct tttaggcaag attgatatta
    12661 ttaccggaac atatggcaaa gcgcttggcg gttcagcagg cggatttgta gcaggccggc
    12721 aggaagtaat agacatgtgt attcaggctt caagaacatc actgtttaca aacagccttc
    12781 ctcctgtagt tgctgctatg gctttaaagt ctgtacagat tcttgaaaga gacccgagta
    12841 taatagagtc tttacataag aaggttaaat atttaaggca gcggttgcat gaagaaggtt
    12901 ttgagccttt ggaaggcgac agcgctatcg ttcctataat agtcaaagag acttcaaaag
    12961 caattaaaat agcaaaagct atgctggaca aaaatgtata tgtaaccggt tttggttttc
    13021 ctgtagttcc agaaggtaca gcacgtatca ggatacaggt atctgatgct ctttcatatg
    13081 aagatataga ttatgctgta aaagctttca aagaagtctt tgacagtctg gattaataca
    13141 tttaatatgt aaagtagaat ataaaaaggg cagatcaagc agtatgcttg aaaataaact
    13201 atgatttttt ctaatagtat aaaaagtata gtacggtcaa aaggtaaaac tgttttgttt
    13261 accattttaa tattctcatt aacacttgca cttgccatat gcgtaagcgt ctgggccgga
    13321 atagagatgt ttttgaagga atgtgacgaa ttctatacga ctgtaggtgt ttttgaatat
    13381 attggtactg aatatcccaa tgatactata tatgattcat atatggatga aacattggat
    13441 ggttttgatg aagatataat tacaaatcat ccatcggttt tgatgtggga ttcttcaaga
    13501 agatctttag ggtatataga tggattctgg agaacagacc agtacatgcc tcaaagaatg
    13561 ttatcggtgg taatagtagg taatgtttct gattccaagg atgagtttta tactgctata
    13621 gtcatgaaag taggatactc attacttatt gaagaagaaa ctgtaattca tattgacaaa
    13681 gattttggtc ctttggaatc gaatcattac tatatgctgt tcggtgaagc ttattacgca
    13741 aggacaccta ttataaattt tgccagaact gattatgaaa atgcgatagc taatgaatta
    13801 ggtttatcta taccatataa ggtggatatc accaaagata ataatggatt tactacattt
    13861 gaaatnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngttct acaaaggata
    13981 tatcaagcat ttactcattt aatcagaatg aattatatat acttgaaggt cgtgttttta
    14041 atgaagagga atatgaaaat tctgaaaaag tatgcataat atctcagtta atggcacaga
    14101 ggttaaatgt cgggatttcg gacaagataa atctgtctgt ttctacatca aaccatccaa
    14161 aacttgatga aagctattgg gcaagtacag gttttgataa atcggatgag tatgagattg
    14221 tgggtataac aaacgcaaca ggagacatgt tctggtatgt atatgttccg tggtcagaga
    14281 acatgcctta ttcttcctat cctatcggtt atactatcgg tcaggcagtt atagaaaacg
    14341 ggcaaggagc acagttctat aaagatatag aacctttgct taaagataga gtaaggctga
    14401 cagtatatga ccagggttac tcaagcgtgg aagttccata taccacatta ttgaatgtag
    14461 ttaagatact tacagcagtg tttgtactgg tagaactggc agtcgttgta ttcttcggtt
    14521 atctttttgt atacagacaa aaggaaactg cagaaactat gtttatgcta ggctcgggaa
    14581 acacaagagt attttcgtat tttcttttca gttcagggtt cattgcatta gccgcatgtt
    14641 cttttgcaat ggtcggcgga cggcttctta gcggattaat aatgtcagcg gtaagaaaaa
    14701 tagctgaata cttttcttta atagattttc gattttcaaa tgcaaattta agtatatcaa
    14761 aaactttgga attcaatcct gatatcggat gggatatatt tataatggtt tctgcaacag
    14821 tgctcataag ctgtgttata tcctgcctgg tttttttaac tttgggattt ttaaacaaat
    14881 cagggaaaac taaaaagata gtcggtccta aaaaagaaaa gaaatccatg aataatccaa
    14941 gtaaaagcgc aaaatatgct tttctgtcaa tattaagagg tggtccaaga acagcagtag
    15001 ttcccatgct tgctcttaca gtcgttcttt tctttggcca gttgtctgtt gtaatgcagt
    15061 cttcgaatga aagaatagag tatatatata acaacaatgt agtggaagga tactttacag
    15121 attacaaagg aaaacaaata gcaaaacaga atatatcagc atataatatt gcaaatttac
    15181 agcattcagg atacatagag gatttgtgta tgaccgtttc tgaaccttca tattatgtcg
    15241 gtgctgtaat aaaagatggg aagatatctg atatagagcc tttgtatgtt ccatctaacg
    15301 cctttgttat ggagtcatta caggcacaaa tatccagagg gccaaatata acatttacca
    15361 ataatataga aaaatctccc gaattctatt atgtggatag tatagatata gattatgtag
    15421 aaggttatga tgaatcagtt ttctctgctt ttgaaagtga agagacatca atatgcttag
    15481 tgactcagtc atatatgaaa gagcataatc tttcatatgg ggatgtcata aggttagcgg
    15541 ttgataatag gattaacagt ttaaagtata atggcaggat attccagcat tatgatttga
    15601 aaattatcgg ttcatatgtt aaacatggtg acaaagatac tatatatgct cctttatctg
    15661 aaaattttaa tacttcagct atattgctaa aggcagatat ttcagataac caagatgatt
    15721 ttgacaatat tcagaccatt acaccgcaac agaaaaaata cctattagat gtaaatctca
    15781 acagtgttac atttaagata attaatacaa aaagcttatc tgaatttaaa gatttctcag
    15841 gggtatatgg gttttctcag gtaaatagaa taagcacaat aagagagttt atggtgctga
    15901 aagacgctat tttaaacagt tcattggcaa atgtcgaaca gcagataagg tacttgaata
    15961 ttgcatatcc aatactttat gttcttattg cagtagtagc atatgcagta tcatatctga
    16021 tggttgtaag cagaaaaaaa gagtttgcaa ccatgacagg attaggagct gagaagaaaa
    16081 aagtatttat gagtttcttc acggagcaaa cgcttttatg ttttatagga acattagcag
    16141 gtatagtaat ctggtacata ttatttaatc aattatcatt gatgcatctg gtattaactg
    16201 caggatttat tcttttctat cttttaggaa gcgctgtttc cataaagata atgggaaaaa
    16261 tggatgttct tacaataatg tcagaccgcg attaaggaga gtattatgcg tattttagaa
    16321 cttaaaaacg taattttcag ctatatgagc aaatacaaca aaacagaggc tgttaaaaat
    16381 gtaagttatg cttttgaaag aggaaaggtt tatgccatag ttggcaaatc aggaagtgga
    16441 aaatctacac tgctttcttt acttgcaggg ttggatcttc cacagtcagg gcaggtgcta
    16501 tttgaaggta taagtacaga taagatggat ttagcgcagt atagaaggga acatgctgca
    16561 atgatttatc aaagtttcag actatttcct ttacttactg taacagaaaa tataatttat
    16621 cctatgggat taagagggtt taaaggaaaa gaagctgttg ataaagcaaa gcagctggct
    16681 ttaaaaatgg atcttcccga aactgtactg aaccgtttcc ctaatatgct ttctggtggt
    16741 gaacagcaga gagtagctat agcaagagca ttatcgatgg aaacaaaagt attgcttgca
    16801 gatgaaccta caggcaatct tgatgaaaag aacagtgtga atataatcga tatattagta
    16861 aggcttgctc atgaagacgg atactgtgtt attatagcta cacatgatct aagtatttta
    16921 tcaaaaatgg atgatgtcct ttacatgaac agtggaatgc tcaggctgga agaattctag
    16981 ctaataacta ctagaccttt ctttatcttt gcactgattg cagatatagt ttggatcaca
    17041 ctttaattta catgtacgcc ttccaagcat catgcgtact ttcatagcct gttttgcaca
    17101 gcatggatca taatatatgt aaaactcttc ttttctcatg tcatatataa ccctgcctct
    17161 tggatagaaa tcgaagtcca catggtattc cgatttatag tgcctttccc atacatccat
    17221 atgacttctg ggataattcc tcatttcagc ataatgctca gcattttcca gcctgcattt
    17281 atgaagtagt attttatcat caatgacaaa aaacaaagcg acaaaaggtt cactcatcgc
    17341 cgcctcttaa gataattgca tctctgtctg gtccaacaga tacatattta actggacagt
    17401 ttatcttctt ttctatgaac aatacatatt cctgagcttc ttttggaagt tcttcaaatt
    17461 ttcttatacc tgatatatct gttttccacc catccatttc tatgaatatg ggttttgctt
    17521 tttcaagcac tggagtaagc gggaagcttt ttagttcaac accatcaact tcataccctg
    17581 tgcatactgg aattttatcc atatatgata aaacatccat tttggttaag gctatgcagt
    17641 ctgctttttg gactgttaca ccgtatgatg tagcaagaat atctattgga ccgactcttc
    17701 tgggtcgtcc tgttgcagct ccgtattcat tgccagcttt tctaagttct tctgcttctt
    17761 cattaaacca ctcagctgta aaaggacctt ctcctacaca ggatgagtat gcctttacta
    17821 ctcctattac ataatctaat gatatgtcag gggctcctga gcctattgga ccataggaag
    17881 ccaatggggt agaggaacta gtataaggat agattccata atctatatct ctaagcgctc
    17941 caagttgtgc ttcaaacaat atgctttttc cattcttttt agcttctctt aataataccc
    18001 cggtatcaca aatatgagat ttgaattctg ttgcatattt atccagccat gccattgcat
    18061 catccaatgt ataaggtttt gaattatata cattctgtaa ggttaaattt ttccattcta
    18121 gaatgtctct taagtggttt tttaaatact caggatataa cagttctcct gccattatgc
    18181 cttttttttg gtatttatca ctataagcag gcgcgatgcc tctttttgtt gatccatact
    18241 ttttatcttt taaacgagct tcttcaagtg catctaattc tttatggtaa ggaaacacta
    18301 tcatagctct gtcgcttatt tttaagttct ctgaggatat atatacacca gcttttctaa
    18361 gcttatctac ttccatagaa agatgttcaa gatcaataac tgttccattt cctaatacat
    18421 ttattacttc gcgacggaat attcctgacg gtataaggtt taaagcaaac ttgccatact
    18481 cgtttattac tgtatgtcct gcgttacttc ctccttgata tcttacaact acatcataat
    18541 cttctgcgat gaggtcgacc atacgtcctt ttccttcatc accccaatta attcctacta
    18601 ttgcacatgt tttcatttta tcctccgaac aacatcttct agtatatcat attatcagtt
    18661 aattttcata ttcatgttat agtgccgcaa gaaaaaaaat tactttctaa gtaaaataaa
    18721 ttacatatac atgaattttt atttaacagg cagagtttga gaaatttgat ctttatcttt
    18781 tttggtaatt atattttaaa ttcacaacag tttgattgtg atatacttaa tatataattt
    18841 tggaggtata acatgagcgg agcttctata atcaactgta aatttgatgc taatcttctt
    18901 tattacaggg atgactgtaa tgtcattatc ccttctgctg gaaaaacaag tgaaatgaaa
    18961 gtactatatc ttctgcatgg catgcatggt acctgccatg actggttgac atatactact
    19021 atagccaggt tagcagaaac atataatgta gcaatggtat gtccaaatgc aaggaacagc
    19081 ttctatgcag atatggtgta cggggaatcg ttttacacat accttactac tgagtttatt
    19141 caatatgtaa gaagaatatt cggattttcc gataaaagag agaataacat gatagcggga
    19201 ctttcaatgg gcggttatgg tgctcttaaa atcgcattca acaatccaga taagttctgt
    19261 gtcggaggaa gtttctctgg tgttttggat atggctaata ttgacaacct tctgactggt
    19321 gatgaaaaga ccgataatgt gaataagaca ctgtttcgaa atatatatgg tgaaaaatta
    19381 gatatcgctg gaacaggcag tgatctaata tatcttatag aaaagaataa tactgttaat
    19441 ggatattctg ttaaagattt acaggtttac cagtactgcg gaaaatctga ttttcttcat
    19501 ctgttcaacc taaggtttaa aaaagcagca caaagtgcag gacttaatct caaatatact
    19561 acagatgacg gagcgcatga atgggaacgc tggtctgaac agatagagtt attcttaaaa
    19621 gaatatatta agtagtacga tggttggttt cttagaatta atcattattt ccatttctgt
    19681 cggtgcattt gtttttgtgc ctcttatacc aaatatatcg cttgttgttc aaaaaagagg
    19741 aaaaggcgta ttaaccggcc ttctatgctt tcttttatat ggcatattgt taaaaaacat
    19801 atttatttat cttatacttc ttattccatc agttgatatg gtattgtcag acaacctgct
    19861 atataccttt ttcatagcgc ttataacttc agctttgcat ttggttgtaa gaatgcttat
    19921 taattacaaa aaacctatca atgacatatt tgctttttca tcaggagaga ctttattaca
    19981 gtgcgtatat ataggggtac ctttaactgt gttgaatctt gtatattctt tgattattaa
    20041 tttcggaggt tttgaatatc tgggaatacc agaaaaactg atgtctgttg taactaaact
    20101 tattaatact gattatacga attatctgcc atacattgct tatccttttt tcatacttgc
    20161 tttgaactgg ttaagtttaa ctgtatttat gggtaatcca aatagaaaaa aaagctttta
    20221 tgtattatct tttataattg aatttatagc gttatcaata tatatatatc ttatgcttag
    20281 taaacttcat gaatttaatg ccatatacat gtcagcattt ttcctggttg tattaatagt
    20341 gcttctgata aataaaagga agaaaaaaat caacaaaaac gacttattaa aaaacagaac
    20401 ctcattgaca tgacttaggt tttgtgatta actagatata caattaaata tgcgagtttt
    20461 ttcggtaaaa gcaattttat aatagggaag caggtgcaag tcctgcacgg tcccgccgct
    20521 gtaagaagga gcgattgcga taagccactg tagtaatacg ggaaggtgca tagctaagat
    20581 ctcaagtcag aagacctgcc gaataagaaa gcatacatta cttgcggtcg atgaggaatg
    20641 tgaaatatat ccttttcctc cgtaagaaaa ggttttttaa ttttaacgga ggattgaaat
    20701 tatgaaaaaa agttcaaaac acatctgcct gattctcgta ttaacgatga ttctggcaat
    20761 catgccgaca gtctcggcgc agacaactga ctacgcatat gtgcgtatcg aaggttatga
    20821 agaaacatta gtagaaaaaa cagcagtacc attagaagtg ttcagtcttg atgaatacgg
    20881 tattagtgac gcattgaatg agtatacggc tctacatgcg attattgcag ctttaaatgc
    20941 taacggttat gacgcttcag ataaggctgt aattgataca aataacggca tgttcgtatc
    21001 cagcgtattg ggtttaaaag cagaaggcaa tgccggctgg atgtatgtac ttaacgatga
    21061 atcgtcttgg aattccatta aggaccagac tatatctgca aatgactaca tagtcttata
    21121 ctacatagaa gactggatgg cttgctcata ctctacattc ggcttatcag atgattatga
    21181 aacagatgca ttcggtacag taaaattaaa cttaaagcat caggttaccg atcctgtgac
    21241 atgggaaaca tcatatgaga atctttctgg tgcttcaata tatattgatg gagagaatac
    21301 cgatatactg acagacgaaa acggtgatgc ctatcttacc tttaccaaat ggggtacatt
    21361 cgatgtatca gcagcaaagt atgaagacgg gattaataca atttcaaggc ctagtgcttc
    21421 tatcaaagtt tatggtcgtg gtgtattagt aaagatttac actgataaat ccgaatattc
    21481 tgcatacatg gaccctaaag ggtttgactt gaaagagtat aatgtatcat ctgaactgga
    21541 gttttcagca ttacatgcag ttattaaagc tttgttaatt aatggatttg acgcaaaaga
    21601 ccaatcagtc aataacttta ataaaggctc atatatatca gcattggcag gaatcgaaac
    21661 tatgggtaat tcaagctgga tgtatacctt aaataatgaa tcttcatggt atgctttgaa
    21721 tgaacaacct ataaaagaag gtgactattt agaggtttat cttttaaatg actggatgag
    21781 tgatacttat tcatattttg atattaagaa tatatcgata cagggcggag aaaaaattaa
    21841 actaagactt tacaggcagg taatggatta ctcaacatgg gaagttacca ctgaaccgta
    21901 tgcagataat gagataatga taaacgggaa aagaagcgga cttgtaacag attcgaaagg
    21961 atatgtaaca ttgagtttta atgaaaaagg agtatatgaa atatccaaca ctggaaaaca
    22021 gcagaacgta tcatatacta atgcatatgt tacagtacag tctgatagaa cagtaaatac
    22081 tgattatttt aatgattatt ctgatgtatc agatgaatat gctgaataca tgcaaaaggc
    22141 tattgaacgc tttattatat ccggagatga gaatttgaat ataagggcgg atgataaagt
    22201 cacaaaagca gaatttctta ctatggtaat acgtgcttca gagcttgagg tatttggaag
    22261 atacaataaa aagtactcag atgtattgtc tggaaactgg tttagcggtt atgctcagac
    22321 ggtattcaag tacggattag cagaacatga caatggatat atatatccta attcagatat
    22381 aacagacaaa gatgctgaac ttgcgcttaa cagggctttt ggtaaaaata cctctttaac
    22441 tgacgagatg ttattagtat tcaatgacgg attaacaaga caggaagcag catatttaat
    22501 aataagctat atggagaatg taaacagata atgaaaaaag cagtgtccct aatactttta
    22561 atatgcttta tgctatctat actgccatcg tatgcgtcag atgatacatt gaatgcaatt
    22621 acagatatat atctggacaa agaagagctt tccgtatggg aagctctggg tcttgctttg
    22681 aatggaaatg ttgttccaga aagttactat gatgcattag gccaggatat tttacaaaga
    22741 gacggagtat acaggacagc gcttgagtat gcaaaaatag ctattctgct taaacaggat
    22801 aataaaaacc ctaaggactt ttacggatat gatattattt catctattgc gaatttcaag
    22861 gatatagaca aatcgggtat taatggagtt atattctcaa tttttgcatt agtagatgtt
    22921 gacattgaaa atgatacagt ctggaatctt gataagctgt tagatctatt acttggttac
    22981 caaaatactg atggaggatt tccgctggca aaaggatggg gttctgataa tgatcttaca
    23041 gctatggctg tgtcagcgct ttcttattac aaagacaaca ataaagcaga aacagcttta
    23101 tcaagtgctt tggggtattt aagttccaaa cttgatagtg acggatttat gtattatcag
    23161 aattctgaca gttcagagaa tctttctcaa atgataattg cattaagttc tgcaaatatt
    23221 tcattgagcg atgagcgttt tgtacgtaat gggaaaacag tttatgatac tcttttggaa
    23281 aagtatatga ctgaagatgg caagtttaag cattctttct cagctacagc caatactata
    23341 gccacagagc aggcttatct tgctttgagt tctatgaaat acggttatgt atatgcatat
    23401 aaggagcata tagaggaacc ggtagaattg cctgatgagc cagaacctgt tccatcagaa
    23461 aatgaggtcg aagatacaat atctgaacca gaagttgaaa aggtattgcc atttcctgat
    23521 gaaactgaag tatcttctca atattatgat gatttagtca cagcttatga gctttcactt
    23581 gtttctgggg atacatctgg aaagcttaga ccattggatt atcttaccaa agctgaagca
    23641 tgtgtattgc tatataaggc tgcaggtctt gaaactgaca tatttatacc aagatattct
    23701 gacgtgaaaa aggctgactg gtattcaaaa tatgttattg gatgctattt taacggtgtt
    23761 gtgcttcaat caggtaatgt actgtttcaa cctaatgaga atatcaaaac agaggatttt
    23821 gaaaaaagta taaatgcaat attaggtgct gatgttacat atagtgatga gttgtacata
    23881 acacgtcaga aagctatttc aatgattatt gaaagtataa aagaaggtgg aaaatgatat
    23941 gaaaaagata cctgttgttt taatgatagt tctgcttttt ttagtccttt taagttcatg
    24001 tacacaaaaa gtactggaag acaaaggcaa aataacaatt actatagatg ctacatctgt
    24061 tgataaagga tttattctaa ccgactatac agtgagcctt aatgaatatg ataccgtgtt
    24121 tgatgttaca ttcaacgcgt gtaaggataa taatatattg atgtcttcaa gaggagtgtc
    24181 agctgcaaga tatgtagaag gtatagctga tttatatgag tttgattacg gtccgacgag
    24241 cggatgggtt ttttatgtta atgaggaatc tgcttctaaa agcagcggag tatatgaatt
    24301 aaaagaaggg gatgtagtaa ggtgggaata cgtaacagag tgataagtcc gttttatatt
    24361 tctcatccag ttacgctgtt gacatatttt acagctttac tgtcctgcat attcatattt
    24421 aaaaatcctt tattcacagc agttgcattt attgtatgtt gtatattagc aatttactat
    24481 gcaggatttg accgatttct taaatctgtc agatggattg ttccaatagg agttcttgtg
    24541 cttattctta atatttttct tgtacataga ggaagcacag ttttatttta ctttttgtat
    24601 aatcccataa caaaagaagc tatgatatat gggatatata atatgtttat gcttatgtct
    24661 gtaatgactg catttatttc ctttaatgcg cttattgaca gtacacgctt tttatatctt
    24721 ttctcaaatg tattaccgaa aacttcattt gtgtttgata tggcattaag gtatattcca
    24781 ctttttaaaa aaagagcttc tgatttatca gctgttcaga atgtaaataa caaaactaat
    24841 caaaagaata ttaaagacaa gataacaaag tcaggaacat atctaaaagc attgactgca
    24901 tggactttgg aagaaggaat ggatactgcg ttaagcctca aagttaaaaa ctatggaact
    24961 agaaaaaaag tacattatgc aaggtacaga tatacgataa gggatatcat actgactgta
    25021 atctttttat ctttttcagt ttttatttac atcaatgctt ttttaggtga aactgatttt
    25081 gtcttttacc cggttacaga tagactggaa atcaaaggat acacccttat tatattttta
    25141 actatgactg tcacaatatt ttttccattt atactggaag ggtttacagc aattaagaga
    25201 ggatgcacaa aaatatggct tcaaaagcat taataagcat taaaaatatg ggttttacat
    25261 atgataacta catacaaggc aaaaccagaa agaaggttgc tttaaaaggc ataaatctgg
    25321 atatatatga aggagagata ttgctcataa tgggaccttc aggctgtggg aaaactactt
    25381 tgctgagaaa tctaaagaaa gaagcagcac cttatggaaa tcttagcggt aaaatagaaa
    25441 gcttagttga tagcagtaaa atatccattg tattttccaa tccggaaaca cagctggtca
    25501 ctacttccgt tttaagcgat cttgctttat ctatggagaa cctgtcatat gatgcatata
    25561 cacaaaagaa gagaatgagc gagacagtta gttactttgg cattgaggat cttcttcata
    25621 ggcatcctga caccctttct ggcgggcaga agcagataat atcattgtgt tcacagctta
    25681 tggtacatcc tgagatttta ctgcttgatg aacctgtaag ccagctggat ccaatatcgg
    25741 ctctggagtt tacagatacc ctgagaagac ttaatgaaga acttggtatt accatagtta
    25801 tgacagaaca caggcctgat gatgtaatat cattagctca cagacttgta tatatggaac
    25861 aagcagaggt agtttatcag gatgatacgc gtaatacgct taaagacttg tggttaagcc
    25921 atgaagtaaa aagaaaagat tttcttcctg ttgtttcaaa gtgttcgtta tttcttgatg
    25981 aaaaaagacc aacatgcatt actccaaaag agttgaaatt aatggctaaa agcctggatt
    26041 tgcccactaa acagatacaa aaaagacatg atgattttat aaatgctgaa aaagtagtta
    26101 agataagaga actgtttttt gcatatgaca gagaaagata tgctcttaga aatctcaacc
    26161 ttgatatatc aaaaaatgaa tttttgtgta ttatgggtag taatggctca ggaaaatcca
    26221 ctcttttgaa aataatatcc ggtatattga agcctcatat cgggaaagcg aaagcttatt
    26281 ataaacagat acagtatatg ccccaggatt taaattcctt ctttacagaa gataccgtac
    26341 acgagcagat ggcattttcg gaaatggatg aatcttatta tatttcatta accaatcagc
    26401 ttaatttaga agaactttta cacaaacatc cttatgacct ttctgcagga gagcagcaaa
    26461 aagctgtact ggcatgtatg cttttgaaaa aacctgattt aatcatactt gatgaaccga
    26521 caaaaagcat ggacccttca ctaaaaagga ctatatcggg attattgaaa acctccgaag
    26581 cgactgtagt ctgcgctaca catgatcttg agtttgcagc cagttatgca acacgatgtg
    26641 taatgctttt taatggagag atagcatatt ctatggatgc aaagagtttc tttaaggaaa
    26701 acagatatta tactacatca gtaaacagat cttttggtca gatagattct aatcttgttt
    26761 tgtttgaaga catcgaaaat gcctatggag gaaaaggtga atagaaagat ataccttttt
    26821 atagtttcat ttatattgat tgttatagct gtcatagtat cagctaaaag catggctgct
    26881 gccttgatac tggctgtaac aggtgtgctt ataatcggat ttagtatgtt tgaaagaagt
    26941 aaggttacag cggaaagaat ggttctttta tcaataataa cggcagtttc ttcagcgggg
    27001 cgtatgcttt ttgctgctgt accaagcata aacccttctt catttatagt tatcatgtct
    27061 gctatggtat tcggtccttc atttggattt atgactggag ctctcactgc tcttgtttcc
    27121 aatatgatat taggacatgg cccatggact ttatggcaga tgctactttg gggacttatg
    27181 ggcttcttgg ccggaatgtt ttctaaatct tttgaaaaac atatctggat aagattagcc
    27241 tatggtttta tatggggttt catattcggt tggggaatga atctttttta tgtagtttca
    27301 gggtatataa cagaaacagg attcaaggct tttttaatag caagcgctgc atcttttgtt
    27361 tttgacttaa tgcatgctat aggtaatctt cttcttattc tattgcttgg aaaccgattt
    27421 attaaaatat tcaacagaac agctcttaaa tacggtctta aagaccctca aggaggatct
    27481 tgaagtattc cttttaaaaa atagtaaaat taagggtaat atatcgtaca atgacatatt
    27541 aatacatact ttacaaacat atgttcgtat tatacaatat acctgacgat gaaggggtat
    27601 tatggaaagt ataatattac atgctgactt gaatagtttt tatgcttcgg tagagtgtct
    27661 ttataatcct gctataagga atcttcctgt tgcggtgtgc ggaagtgtag agaacagaca
    27721 cggaattgtt cttgccaaaa atcagccggc aaagatggtg ggaataaaga caggagaagc
    27781 gatatggcag gcaaagctta aggctgccaa tcttttagtc gttcctcctc attatgacag
    27841 atatctcaga ttcggtaatc tcgccagaaa aatctacggc acctactcgc aaagagtaga
    27901 accttttggc atggatgagg catggataga tatatccttt ccaggcagaa gcattagtga
    27961 tggttatgag atagctaatc agataagaga aagaattaaa tttgaattag ggataactgt
    28021 atccataggg gtaagcttca acaagatatt tgccaaactt ggatcggata tgaaaaaacc
    28081 tgatgccgtt acagttatat caagggaaaa ctacaaagaa aaagtatggc ctttagatgt
    28141 aggggagctt ttgtatgtag gaagagctac aaaaagaaaa ctcagaaact ggaatataaa
    28201 tactataggg gatttagctc aggctgatgc tctttttata cgtaataaac ttggcaaatg
    28261 cggtactatc ttaaaggatt acgctttggg taatgactat tcccaagtgg agaatacaga
    28321 gcatgagaca ataataaagt ccataggaaa cagcacgacg ccaccaaagg atttgtgttc
    28381 tttggaggac atacgtatca cttcttttgt tttgtctgaa agtgtagcca gaagattaag
    28441 agagcatggc ctaaaaggaa aaacagtaca gatatatatg cgagactgcg atttggaagg
    28501 ttttgaacgt cagggccagt taaaagacta taccaacata tcaactgaaa tagcacataa
    28561 agctattagt cttgttgagg ctaactggtt tttcaataaa cccataagaa gcttcggagt
    28621 gagggtaagc gaccttacat cttccaaagg gccaatacag atatctatat ttaaagagga
    28681 aaacaagcgg ttcaaggtaa tggatttgga atattccata gatacaataa gaaaaaggta
    28741 cggaccatat gctatacaaa gagcagcgct aatgcaaacc agacagttcg gagatctttc
    28801 tgataatacg gaagctctga tgagggaagt atcatttaat acaggagaag ccagatgacg
    28861 gaagataaaa gaataaagga atacgttaac gttacagtta aatttaaaaa agatggaagc
    28921 atgattcctg ttagtataga gtgggagaac gggaaagtat atactgtaga caggatattg
    28981 gatgtgcgaa atgcagccag tctaaaagca gggggttgcg gaatgaggta cttatgtaag
    29041 ataatgggta cacagacata tatatttttt gaagaggaca agtggtttgt ggaaaggaaa
    29101 gcgcaatgtg tggaagattt ttagatttag aagcaagaga gattttccca tcggaccttg
    29161 ttgaggtaga gaccattcag ggaaccatgg acaagatatg gggtgtagtt aacaaataca
    29221 gcaataatac tatgatcaat gcaagaagcg agacggtaaa tgagcttgct atgttcaaat
    29281 atatgaaacc gtgtgttatt cctgctattg gttatttcga gtgggataag gataagaaaa
    29341 aatatctttt tactaaaaaa aatcaaactg taatacatat ggcaggtgta tacagagatg
    29401 acagatttgt tattataact aaggatgctt atgaacaatt tgctcctatc catcatagga
    29461 tgccgtatat attatctgct gttgatatac agatttggtt tgatgaaaaa aaactgtttt
    29521 acagggacga ggaattggtt tgcaaaatag cttaggggga aaagaaaatg gctaaatcag
    29581 ctgaacagaa agctaaaata ctgcatatat ataagatgct gtatgagaga acagatgaag
    29641 agcatacgat aactataaat gagattatac agtcattatc atcacttggt ataaatgcag
    29701 aaagaaaaag catatatgat gatataaggg tgttaagaga attaggattc gatataatcg
    29761 gagtcaagtc caaaaactac agctattatc ttggttcaag ggagttccag atacctgaat
    29821 taaagcttgt aatcgatgca gtggtgtcag cacgatttct cacatctaaa aaaacaaaag
    29881 aactgataga cggattatgt aatcttgtat ccaaatatca aaaaaaagag ttaattcaaa
    29941 atacaggtat atatagtaaa gctaagagtc agaatgagca tgtatactat aatatagaca
    30001 gtataaatga agctatatcg aaaaacgcaa gaattaaatt cagttatttt gattatgata
    30061 taaacaaaga aaaggtttac agaggagtaa aaggcaaata caaggtttct ccttatgcgc
    30121 tgatatggga agatgattgt tattaccttg tctgttatta tgaaagatat aatattactc
    30181 agttcaaggt agataagatg gaatcaatag agatattaag agaaaagata gatatggaaa
    30241 aaagagaaag gtttgacctt tcagcttaca caaaacccat gttcaatatg tttaaagggg
    30301 atcctgtaaa agctgaaatc atatttgaca tgtctctatt aaatgttgtt tttgaccggt
    30361 tcggtctgga tattacagta cagcctttaa ctaaaaataa atttaaagcg tttttaaatg
    30421 tatcaattag tccgaccttc atttcctggt tatttacttt caaagataag gctgaggtaa
    30481 ttgctcctca aagtcttgta gatgagatta agaatacaat agacaaattg tctaaaacat
    30541 attcttgcta aaatagctta aaaaactgct tgccagttat tgttgaatac tgacaggcag
    30601 ttatgtttag ttagcgcaag tttaatgctg agtattaatc aacttccctt atatctgtta
    30661 ttacagaccc gccatttaca attacccatc cccatttgag acgagcttcc atttccattt
    30721 tctctaaaag ttctggagtt atggattctg atatgattct gtttgcttct gcttcagcct
    30781 gagcttcaat aagtttgatc tctgcctgga ttgacgcttt ttccttgtct gcagaagcct
    30841 caataactct ttgctgtgcc tctatctgaa cacgtaagcg ttcctgctca gcagtctcga
    30901 cacgtttctt agctatagct tcatcctgaa ttgctttttc gatttcttca cctgcatcag
    30961 tatcagaaaa gttaatcgcc acaaattgaa taccattgat tgcaaaacgt ttagtcagtt
    31021 cctcttcaat tcccatgtat aaattatttc tttcagaacc taatagttca ataatgttgt
    31081 actgggttga gatagcttca atacttcttt gaacagtcgg tgaaacgagg cttgttgcta
    31141 cattctgcaa tgtacgatac tgtttaaaaa cctcgaatgc tttttcaggg gagactttgt
    31201 attttacatc tatagtcatc tcaacatact ggctgtcctt agtctgacct gttattcctg
    31261 ttaaattact tgtctggact tcggtgctta ttatgtaaat tgtgtcaaac acacctttaa
    31321 agtgccatcc ttcaggtaag gtttcttcct tcactcctgt gaaggggctg tactggactc
    31381 ctaccgagtt tgcaggtatt acagctattg atgctataaa tattgatatt atcaaaggaa
    31441 gtactgctgc tatgcaatta gccttgatct tcttactgct atcttttttg ttattgaaat
    31501 agattataac cgctaataat gcaattgata ttattaatcc agctattaat gttgtcatat
    31561 ttcacgctcc tttttcatat ttgtaaaaag tataaatact ttttaactca tacaatatta
    31621 tatgtttata aacatcaagt caaatacctt gtaaacaaaa atttaaatga taaaatagat
    31681 ggtaggagga tgctatatgt catatgagtt gggaatgcag gctattaacc ttgaaatgcc
    31741 tgatatagta ccgaggaccg agtatgctta tcaattagct tatggattgc aaaaagcggt
    31801 tactggaatt gatgttaatc aggattcaga tggagaaact aagtttaagg cggtaacttc
    31861 atttgaaaag atttgggata tttcattgtt ctgggcaact aaaatcggta gtggtatttt
    31921 tggtgataaa agaacaaaaa tgggacatgc cggttttgaa gaaggataca aagactttat
    31981 agacaataag catcaggctt tcacagattt agatgatatt tataatatgg actttgataa
    32041 gacatatggc agtatcaata tagatgagac tgctgatatg ttttacaatg atttattaga
    32101 aagaagcaga agacatccta caggggtaaa tacgactggt atatatgtta catgcatatc
    32161 aggcatgctg gaaatactcg gatgggatat gcttctttta gcggcagggg aagatgcaaa
    32221 aagatttgga gaatttacca taaggtattc gaaatggata tcgcagtatt tcaaagctct
    32281 tgctaaatca aaagtgcctg tttgtatgat tcatgatgac atggtatggt cctccggccc
    32341 gtttatagct ccttcatggt atgacaagta tgtattccct tatgttaaaa aaaatatttc
    32401 atatctcaaa gaagccggca aaaaggtgct tttcacatgt gatggaggat atgaccagtt
    32461 tattgacaga tttgcagaca tgggaattga tgcgtttatt tttgaacctt taactgattt
    32521 ggaatatata tgcgaaaaat atggaaaaac tcatgccata attggaaacg cagatacaag
    32581 gattataatg agaggaacta aagaagacat agaaaatgaa gtcagacgct gtatggatgc
    32641 agggaagaaa tgcccaggtt acatgtttgc actgggaaat cagatctcgc cagggacaaa
    32701 gattgaaaat cttctatact ataatgaaat gtatgagaag atgaaaaaaa ggtaaggaat
    32761 atgctttgca ttgacttttt tatagtgcaa acatattata aatatatgat ccaaggagcg
    32821 taaaatctat gaaaacgaca cctttaacac cggtgcatga atccttaaag gccaagatgg
    32881 ttgactttgg cggtttttat atgcctgtac agtacacttc cattattgaa gagcacaatg
    32941 ccgtaagaag cagtgcggga atatttgatg tttcacatat gagtgagata acagtaaaag
    33001 ggaaagatgc agctgcatac atctataatc ttgttacaaa tgatatcagt acagccacag
    33061 aaaaccagat aatgtattca cctatgtgct acgaacatgg aggctgtgta gatgatttat
    33121 taatatacaa gaataatgat gaagattttt taataatagc caatgcttct aatacagata
    33181 aagattatga atggatgctt aataatgcct atggctatag tgttaaaatc gaaaatgttt
    33241 ctaacgaata cggtcagata gcattacaag gaccaacagc tcagacttta ttacagcaaa
    33301 tcacagaatt tgatctggat accatacctt tctacagatt tagaaaaaac atagatttaa
    33361 gcggcatttg cgcattagtt tcaagaacag gatacactgg agaagatggt tttgaaattt
    33421 acattaaagc agatcatacc gaatacctat ttaaaaaact tctggacctt ggagcagtcc
    33481 catgtggatt gggagcaaga gatacattga gatttgaagc agctcttcct ttgtatggac
    33541 atgaactatc agataagata actccgctaa atgcaggatt aaagagtttt gttaaatttg
    33601 aaaaaacaga ctttattggg aaaagcgctc ttgctgaaca agcgcaacag ggtcttgatg
    33661 taaggtctgc aggcttggaa atgattgaca ggggaatacc tagaaacggg tacgaacttt
    33721 ttaatgagtc taacgataag ataggatatg taacaagcgg gagcacatgc ccttctttaa
    33781 acaagaattg tgcattaggc ctgattacac ttgaatatgc gaaaatcgga aatataatat
    33841 atgttgatat acgaggaaaa aagttaaaat cggttgtcgt aaagaagccg ttttataata
    33901 aaaagtataa aaaatgacaa ggagtcaatt atgaaattca ccaagaatca tgaatggatt
    33961 aaaaaagaag gggaatatgc ctatatcggc attactgatt atgcccaaaa acagcttggg
    34021 gatatagttt atgtcgaatt ggcagaagaa gatcttgtat tacagcaagg agatgttctt
    34081 tgtgcggtag agtcagttaa gacagcagca gaggtatata tgcctgttag cggcagagtt
    34141 gttgaagcta atcatgaact ggaagatgct cctgaaaaac tgaatgaaaa cccatatgat
    34201 gcatttcttg ctaaaattac aatggatgat gaatcagaat atgatgagct tatgactgaa
    34261 gaggaatata acaagttcct tgaaagtgag gaagagtaga atgagataca taccggtaac
    34321 tgataaagac cgggagcaaa tgctgaaaaa gtgtaatgct ggaagtattg aacagctttt
    34381 tactgatata cctgataagg ttagactgaa agaaccttta aaacttccaa aagcaaaaag
    34441 cgagatggaa ttaattaaat atatgcaatc tctttcttcc atgaacagca acacagatga
    34501 ttatgcttgt tttttaggag caggtgcata tgaccactat atcccatctg tcatcagcca
    34561 tttgatttcc cgacaggaat tttttactgc atatactcca taccagcctg aaataagcca
    34621 gggtacgctt acagcaattt tcgaatatca gacaatgata tgtgaactta caggtatgga
    34681 tgtttctaac gcctctatgt atgatggcgc atcagctttg gctgaagcat gcgtaattgc
    34741 ctcctcagcc tcaaagaaaa agcaggatat actgcttgta gaagatgtac atccacaata
    34801 ttcagaagtt gttgagacat atcttaaatt tagaggaatc aatcttaaaa aggtaactgt
    34861 tgaacagtta tacgaaactg atttatcaca aacagctggt gtatgtattc aatacccttc
    34921 attttacggt gatatatacg atatggaaaa aatcggtgaa tatgtaaaaa atagcggagc
    34981 tttgtttata gtctgtgcaa atccaatggc tttagctatt ttgaaatcac caagtgaatt
    35041 tggagcagat ttcgttgtcg gtgaaggaca acctttggga attccattag gatatggagg
    35101 cccatatctt ggttatttcg catgtacaaa agagcacatg agaaaaatgc ccggaaggat
    35161 tgtaggaaaa acagttgatg ttgacggaaa aactgcatat gtattgacat tgcaggcaag
    35221 agaacagcat ataagaaggg aaaaggctac ttccaacata tgttctaacc aggctttatg
    35281 cgccctaagt gctactatat atttatctgt tgtaggaaaa gaaggattta agcaggttgc
    35341 aagaagatgt ttggataatg ctcattacct gtataacgga ttacttgcta ccaataagtt
    35401 ttccaaggta tctgatatgc cgttctttca tgaatttaca gttaaaccta aagaagatat
    35461 atcgaaattg aatgattatc tttttgaaaa tggatatata ggcggactgg aaacagaaaa
    35521 gggatggctt ttagctgtaa cagaaaaaag gacaaaagaa gaaatggatg actttataaa
    35581 gaaggcaggt gagttcaatg gctaatctca tatttgacct ttcaaaaaaa ggaaaatgcg
    35641 cattttcgtt gcccgaaagt cagataccaa cttataatct tgatcaaaaa tatctaaggt
    35701 cagttgatgc tcaccttccg gaagtttctg aagtggatgt aataaggcat tttactaata
    35761 tatcaaagcg taattacggg gtagaccagg gattttatcc tttgggttct tgtactatga
    35821 aatataaccc caaactgaac gaagatgttg ctaatttaga cggattcaaa aaactgcatc
    35881 catatcagca aagctctcag acacagggag ctctaagagc tgcttatgaa ttacaggagt
    35941 atctttgtga gattactgga atggcagcgg cgacactaca gcctgcagca ggtgcccacg
    36001 gagagatgac gggcataatg ataattaagg cttatcatga aagcaggaac gattttaata
    36061 ggacaaggat gattgttcct gattcagccc atggaacaaa tccagcatct gcagcaacag
    36121 ctggttatga tattgcacaa gtcaaatcaa atgattacgg acttgtagat attgatgctt
    36181 tagagtctat gatggatgaa aagacagcag gacttatgct tacaaatcca aatacattag
    36241 gattatttga acgagatatt aaaaaaatcg cagaaatagt tcacaaaaaa ggtggtcttt
    36301 tgtactatga cggagctaat ttaaacgcga taatgggtat cgcaaggcct tttgatatgg
    36361 gttttgatgt ggttcacagt aatctgcata agacaatgtc cactcctcac ggcggcggag
    36421 gccctggcag cggaccagta tgtgtgagag aggaccttgc aaaattctta cctaaaccag
    36481 ttattattaa aaaagatgat gtatatgaat tggattatga caggagtgat tctattggaa
    36541 tggtaagatc attctatggc aattttttgg tttatataag agcattgtca tatattctta
    36601 ctatgggacc agatggttta acaagagcaa gtgagacagc tgttctgaac gcaaattata
    36661 taatggccag tcttagagac agttttgaga taccttttga cagattctgt atgcatgaat
    36721 tcgtgctttc tggaagtatg caaaaagaag aatatggtgt gtctactctg gatatggcaa
    36781 aaaggttatt agactatgga tatcatccgc caacaatata ttttcctcag atagttcacg
    36841 aaagtatgat gatagaacct actgaaacag aaagtaaaga aactcttgat gaatttattg
    36901 aggttatgaa aaagattgca gaagaagcaa aagaggacgc tgatttgtta catgacgcac
    36961 ctcataatac atatgtaaga agactggatg aagtaacagc tgcaagaaat cctgtattaa
    37021 aatgggagaa ggagtaatca gataattact ttttgaatta gtacagacaa gcaagtagtt
    37081 taatgatttg cagataagag gtatatcatg gaagatgtaa atttaacaca acagttcgtt
    37141 tacaagatga cagaaggaat tataggtgag aaatggcctg taggcaatac ccttccttct
    37201 tttagggatc ttgctaaaga atatggtgtt tcaagaagtg ttataaacac agcaatagcc
    37261 gttttagaaa ataaagggta tgttgagatt gtaacaagaa aaggagctgt tgtaagggat
    37321 tggaaaaaag aaggtacagt aactgtactg aatgatgcta tttctttagg atatttccaa
    37381 ccagatctga tagatagttt tttcagcctt ttgaattatg cagaatgcaa cggagtattt
    37441 gaagctgcat caaaagcaaa agaagaagac atttttgaat taaagataac tattgagaaa
    37501 gaagcagttg aaagaaatgc agagaatctt gttaatcttt atttaaaatt tcatgaacat
    37561 gttgctacaa tgtcaaataa cattgcatat tgtttggtat ttgcttcatt gaaagaggca
    37621 tttactaaaa tagctcagat atatactctt gatggatctg cgaaacagca atgttttaag
    37681 atgcataaag atatatttca gtcgttaaag gatcatgacg gacaaaaagc taagtgggaa
    37741 atgcaggaat tattagatca tttgagagtt ttggtaacat ataagacctg caatctaaaa
    37801 gaaaagtaat aaaattgaaa atacagtaat taaaggaaaa tcatgtacaa tacatgattt
    37861 tctttatgct tgaataaaat gattaaacca agacatttaa ctaataaagg ctgtaacaat
    37921 ttaggtttta atatacatat gattataatg aaaaattgaa aaatattaac aatttatatt
    37981 gacataattt agcacatgat atattatact aataagggag gacgaaaata tgaatatgcc
    38041 aaaaatagtg ccaatagctg agctgaggga tactaagcat gtactgcaca tgtgcagagc
    38101 tacaaaggaa ccaatcattg ttacaaagaa cggatatggt gaactagtta ttatgagtat
    38161 aactgcattt acagaaatgt ctgaggaatt agataaactc agaaagcaag tagcaaaaga
    38221 taataagtat taatacactg ctgctttgca aagaattgtt ttaaaaaaaa gataggatat
    38281 acgctatctt ttttttgtta aaatatgaga tataattgtt tgattattta ttatctcttt
    38341 actctggcat ttcctttaaa tatactccat atgacatctt catcagctgg ctggccataa
    38401 tcagtcatca aagtagttac taaagctccg cttgcccaac cgaactggac ccatttctga
    38461 ggttcccatc ctttaagtat tccatatagg aggccaccaa caaaaccatc accaccgcct
    38521 attctgtcca ttacaggaat ttctctgggt tcaacagagt accagttatt atctgcataa
    38581 ataattgcac cccaaaggtg gctgttagcg ttttcaacct gtcttagtgt atttgcaaaa
    38641 acctgtacat tagggaattc tgcacttgtt ctggttatca tatccttgaa cgcatctgtt
    38701 tttgaaacag tttttccacc tgcttcaggt ccttttattc ctaatgataa ttgaaagtct
    38761 tcttcatttc caataagtat atctgaaatg gcagctattc tagtaaaaat atctctaagt
    38821 tcatcttctc tgttaatcca gaaagaagca cgataattta aatcaaaaga tattgcagta
    38881 ccatattttt ttgctgcttt tgctatatcc agacagcatt tgctgctttg ttgggacaat
    38941 gctgcaacta gaccggagat atgtaatact ctgcaaccct catctttaaa taacctttct
    39001 aaatcaaaat cgtcagcact caaagtcctt ccaacttcgc cagcacggtc attatgcact
    39061 cgagggcctc ttactccgaa tccgctgtct gctatattga actgatgcct gtatccccat
    39121 ggtccgccct gatcgacatc ctttccctcg aaatcaatat tcctttttct tagttcagct
    39181 tttataaaaa gagctattgg actatctttt acgaacttcg tcagaacttt tgcttttaat
    39241 cctaaggatg aagatatgtt aagcacattg ctttcagcgc ttgttgcctg catttcatat
    39301 atactgctta cattgacggg ttgtcggttt acaggggtta ttcttactcc catgcttgta
    39361 accgtcatta tgtcatactt gcaatttttc tttaattcca taaagtaata cctccagatt
    39421 ttttatacat atatattata cataaatatt gatagatatt aatgtaattt gtcagatatg
    39481 agattaaata ttaaatgatg tgtttaaatc agatattaat taatgtataa tttccgttaa
    39541 ggaggagtga ttgtgaaatt cggagtaaaa aatatattat atatagtctt gctaatatca
    39601 atagcggtat ctttatcttc atgtactctt attactcagg tcatagaaag tagtcagaat
    39661 agtaataatc cactattttc acagcttcct gaatttgatc cgtattctac taatcaggac
    39721 ttaaataata ttgatatgtc agatgcggaa tacatagatg acattgagca gttctacatt
    39781 catcttgaaa ccagtatgga aaaaaggcat agaagtatat ttgtccgtgc agcatctcat
    39841 gatgtttttg ctgatttgtc agaattagat aaatacaatc tcaaagggtg ttataaaact
    39901 gtttattcag gatccaatgg aaccttttac gcaatttatg atattgagta ttatcctgga
    39961 gataatgtat accatgcata tgaaactaag gacaaatcac ttttaaatga aaaagagaag
    40021 gaaatatatg tattagcaga gactttcatt gcacagaata ttactgaaca gatgagtgat
    40081 tttgataaag agttggtaat acatgactat atatgcaatg ctcttgtata tcaaaaagag
    40141 ggtatagata cagtagattc atataatatt actggtgctt atgcactcat tacaggtttt
    40201 gcaaattgcc agggttatac agatgctttt tatatgctaa ctaacatggt tggtcttgag
    40261 tgtgaaaagg tgagcggtga atcacaaggt atcgcgcata catggaatgt tattaaaatt
    40321 gaagatatat ggacatatat tgatgtaaca tttgatgata ctgcatttga taaagaaggt
    40381 ctgatatttt atgcttactt caatgttcca aaagactaca tggatagaag ccatatgttt
    40441 tatgattatc aggaactcaa aattgcagaa ggttatgaat atttttacta ctcctataaa
    40501 ggttcagcag tttttaattc tctggatttt aaccggaagg tatatgttcc attatctcaa
    40561 ggagcagcaa gtattgaagt ttttgtgctg attaatgatg tccagccgat gcttgatact
    40621 cttaaagatt tgaacctgtc catgcaactt aattatttta taattaatga atgtacatta
    40681 cttgctataa atacccaaca atgatatatt ccaagatgaa aagagagaga tatgacaaat
    40741 atcaggacta aatcacaaat agataaaatg agagaggcca gtaagatagt tgccgcttgt
    40801 catgaaaact taagagatgt aattaaacca ggtattacaa caatggatat tgaaagattc
    40861 gtagacagat ttgttgcatc ccataatgca atttgtgctc aaaagggata ccacggatac
    40921 ccgttttgca catgtacatc tgtaaatgat gaaatatgcc atgggatgcc gtcatcctat
    40981 atcttaaaag aaggcgatat agttaaagta gatatggttg taaataaaga tggatggatg
    41041 ggtgattctg catggtgtta tgcagtcgga caaataagcg aagaagcaaa caagctcatg
    41101 actgtaaccc gtcagtgtct tgaaatagga ataagtcagg ctgtctgtca aaatcggtta
    41161 ggtgatattg gttatcatat acagtcgcat gcggaaaaaa acggtttttc tgttgtaaga
    41221 gattttacag gtcatggaat aggcgacagc atacatgaag accctgcagt attacattac
    41281 gggcaaaaag gaagaggaat aagaattcag gaaggaatgg tattcactat tgaacccatg
    41341 ataaatcaag gagattatga tttatatatt gatgaggata atgaatggac agcttatact
    41401 gcagacggat ctctttcagc tcagtatgag cacacaatag ccattacagc aaatggccct
    41461 gaaatattaa ctttacagga attctgatat gctccagaaa attaaaacag gctcacaaaa
    41521 aatttatata gctctttttt atagaaacga cacaaagctt cttaccctac caggtaaaaa
    41581 agacagggac tggttcatta tacaggccct gattggtacc gtttttgctt tgtttaccgg
    41641 agcaacctat cttacaggtt tgttcaataa agtaaatgcc ccggaaatac ttattgggta
    41701 tcttccaata atcggaagta ttgcaggagt tgtaactata tttgccggtt atactatgca
    41761 aaggataaga agccgtaaga agtttatcct aatatgcaat accatactta agactcttat
    41821 tgtattaatt gcatggatcc cgctcatatt ccccaatcaa aatgcagctt acataatgat
    41881 tattatggct cttatagcat atgctcttaa taattttatg gcaattgcca taaacagctg
    41941 gtttgttgat ttaattgatt caaaaataag gggacagtat atgagtgtaa ggaacatggt
    42001 ggctcttatt gctaatctta ttgttcctgt tatagtatca gcttttatag acacttcatc
    42061 tgaccagtat cttgcaatag tagtagtttt tacaatagca tggattatta tgtgggcgga
    42121 aagttattca ttctataaga taagcgaacc gccgatgagt cctgcaacaa aaaaaattaa
    42181 gctgaaagat gtattcatag tacctataaa aaataaagat ttcatgaagt ttatggtacc
    42241 tatcgcagct ttctacttca tatggtatat agctatgtca tatggaactc tttatcagtt
    42301 aaattatctt ggtctgtcat atacctttat ttcactagct tctgtattaa atgtaatact
    42361 acagtttata tggtatccga taataggcaa attagtggac agatataaca ctgatttgct
    42421 tcttatggca tgttttatgt tgtatttcat tgactctctt atatggggtt ttactaatct
    42481 ttcatcaaag tatttgatga taattttagt aaatgtgata gcatcaatga cggcaccgtt
    42541 cttttctctt actctgttta aaaagaagta tgatataata ccacaaacag aaaggtcgct
    42601 ttatgatggg ttttatactg caataatagc ttcaataata gcattagcac caataatcgg
    42661 aagcttgctt aaagactata tagcagtaaa tataaaacca ttctggatat ttgaagtacc
    42721 acagtttcag ttgatattca tggtaactaa tatattgctt ttagtattaa tactatttaa
    42781 tttgaaaaaa acaatcagac tatataaaga agcgaaaagt cagggacctg ttgatacaga
    42841 taactaaaat aggaggaagt cgtggaaatt ttaaaaaaac ttacagatgc attcggagtc
    42901 agtggaagag aagatgaaat aaacgctgtt ataaccggac tggtaaaaga ttatgctgat
    42961 gaaataaaat atgacggaat cgacaacgtc atagcagtaa aaaaaggaaa tgggaaagat
    43021 aaaaagaaga taatggtatc tgctcataaa gacgagattg ggtttatgat aatgagcatt
    43081 gacagtgacg gatacataaa tttaagacct attggaggaa ttcctgtatc tttatcaata
    43141 ggaaacagga taatattcaa aaatggaaca gtgggtgttt taagttttaa aaaagattca
    43201 gagaatatta aaaacaataa tgttggtgat atgtttgctg atatcggagc aaaaaataag
    43261 gaagatgcct taaattatgt aaaaccaggt gatgtcggaa gctattatgg gccattgtgt
    43321 gagttgaaga atgatagagt tattgcaaaa gcattggata acagaatagg ctgttatata
    43381 gctattaaaa caatgatgga gaataccaaa ccatacaatg atatatatta tgtatttacc
    43441 tctcaagagg aattaggact taaaggtgca actgtggcaa gccgtgttgt aaaacctaat
    43501 attgcagtag cagttgatgt aacttcatca tttgataccc ctgttacaaa agagggcaat
    43561 tctgttttag gcggaggagc tgctattaaa attatggata gatcagttat atgtgatgaa
    43621 aaattaataa gtataatgca ggaactatgc aaaaaatcag aaattgcata ccaatcagat
    43681 attttggcag cgggaggaac tgatgctggt gctatatata catcaaatga aggagtacgt
    43741 actggtggta tatcaatacc tatgagatat gtgcatggtc cagtaggaat ggttgatatg
    43801 aatgatgtta aggcttgcat aagcctattg aagggcttta ttgggcaaaa aatatgaaaa
    43861 atcatatata tacaataaaa gttattattt tcatatattg aaaagagcaa aactgtgata
    43921 aactattacc gttataacaa tatcgtttat ctagggattt atttaaacag aagggttggt
    43981 gttgtaattg gacgaaaata gcttgagaat ttacaagaag aatgaaaaga aaatcttcac
    44041 caaggtaaat ataatgattt acctgatgat agtgcccggc gtggtactat attatgtttt
    44101 ccgctatatc acaatgcctg gtattatgat agctttccag gaatttacta ctggcggatt
    44161 caagtactgg tgcggatttg aaaactttga caaactgttc ggattaagag ctttttggca
    44221 ggctttcagg aatacatggg tgtttatagg attggaatat gtttttatta aaccatctcc
    44281 aatcatatta gcattattgc tcaatgaagt aagaatcatg tggttcaaga agactgttca
    44341 gacagtatct acgttaccga acttccttac atgggttgtt gtcagcggta tttttataca
    44401 gcttctatca cctagtacag gttacgtgaa tgctattatt gcattactgg gaggcgaaag
    44461 gatatacttc cttggaagag cacagatatt cccatggctt ttaacattca taaggatatg
    44521 gagaggggta ggatattcgt caatcattta tcttgccgca ctcggtggtg ttgatccaga
    44581 gctttatgaa gcagctgtta tcgacggtgc ttcaagatgg aaacaaacac tttatgttac
    44641 actaccagga attaagaaca caatcgtagt tcttttggta ttctcattct ccggtatcat
    44701 gggcggcttg ttcgagccgg tattcacctt gacagacgga aacgcattac ttgatgagac
    44761 tgctatggtt ttggacgtat acatatacaa taccggtatt agacaaggta agttcagtat
    44821 gtcaacaaca accggattat ttaattcaat aatttcaacc attcttatgt taagcgctaa
    44881 ctacgctagt aagtacttaa ccgaagatgg aagaacgata atatagggag aaggtgagat
    44941 aaatgttaaa aagatcatta ggcgaaaagg ttttcaatat attcaactta ttgggaatgc
    45001 tcgtattttc agtaatcatc attgttccgg tattattcgt ttttagaaac tctttcgagt
    45061 taagtgaaac atctaccact acacttagat tgtggccaga acagttttct atggtattct
    45121 acggaatggt attaaaagat gagtcagttt acagaccatt gcttaactca gtattcgtta
    45181 cagtggtagg aactccatta atgttaataa taaacgcatt aggcgcttat acaatgagta
    45241 aaagagagtt accaggcaag agcttcttcg catattacat ggttattata ccaatgctga
    45301 taggcggagg aacaatacca ggattccttc ttatcagatc attaggaatg atagatacac
    45361 tttgggctat aatagttccc ggacttgcat caggttggaa tatgactctt atcagaaact
    45421 attactggtc cattccaaac gaactgacag aatcagcaag aattgacgga gcaccggaaa
    45481 tgacaatttt cgtaaagata atcttgccat tatcaaaacc agttctgtca gcattaggac
    45541 tgttaacagg tatcggacta tggaatacat tctatgcagc tatgatttat atcagaagcc
    45601 ccatcaaatg gacattccct gttaagattc gtgaaacagt tatgaagaac gaaggtaatg
    45661 acaaactgtg ggaagaaatg atggttgcag ctggtcttga cccgacagag cttttaataa
    45721 atcagtctgg tctaggcgga gcgatgatgg ttacgtccat gttaccaatc ttgattgtat
    45781 acccgttcct tcagaagtac ttcgcaggcg gaattatggt cggatcagtc aaaggctaat
    45841 cagtccaaat atataaatat taaatgggat aagcgtaaag gcttatcctt tttaaatatc
    45901 agaatcaatt ctcatatcgc atatcagttg aaccaaatta gcaaaaaagg tatactgtta
    45961 gtaacatata tgaatacgga gattaaagta tggattacag aagaactgat gaaataatca
    46021 aaagaacaaa agcagtatat gaagcaaaaa aaggtaccct tatacaggtg aaggatgtat
    46081 caggtataca agttcccaaa agagctttag acagttttgg attaccagat aacatggaaa
    46141 catatttaga ttatgttata gacaaagata aagcttactg gtctgtaaga gaacagatac
    46201 aagacgatat aataccatca gtaactgccc gttatggtat agctgaacat tcagccttta
    46261 tgggtggtga tgtatcattt acagaaaata catcatggca tcataaacat gttgaggatt
    46321 attcaaatta cactttgagc aaagtagatg aaagtaatat ttggaggaat ctggttattg
    46381 aaggtatgag atatcttaag gagaaagtta aagatgaatt ctttgtacgt tacagaggag
    46441 cagacggtcc tttggatatt gtaaatgcgt taagaggcaa tgatattttt tacgattttt
    46501 atgatgagga agaaggttta attgaactgg cagataagtg tacaaatgca attatatata
    46561 tgcttaaaca gcagcaaaag atagttactg aatacaaagg atatgttata tccggttttg
    46621 atgtaataat gcccaaaggc tatgcaggtc acttgtcagt tgatgctact acaatgcttt
    46681 ctgatgaaat gttcaggaaa tttgaaatac cgtttttaaa taggattgca gataattttg
    46741 atggtttatt ctttcatatt cattcagtag gaagtcatgt tatacctgct gtgtgcgaaa
    46801 ttgataaaat taaattctta gaaatatcaa atgatccgaa ttcacctaga agtattgaag
    46861 tatataggaa gtatgaaaag gaattatctg aaaaaaaggt tatagtaact ttgaattatg
    46921 atgaaataaa agacaatgta gaatttttaa aagacagaaa aacaattatc tggtatgacg
    46981 cgcaaacagt tgacgatgca ttgcgtgcag ttgaccttgt gaggaataaa ttggggtgat
    47041 aacctgaaaa ttgagaaagt acaaaaaagt ataagaaata agctattagg catacttgtc
    47101 atatgcctta ttgctgttgg cttaattatt actgctaaca atatttttat atcaagatta
    47161 gctacaaaca gcaggcaaaa gtttttagag gatactgata ttcagatagg tgatgtgtat
    47221 acaaagtata tcaagacatt tgatttgaca gctgaatcta ttgcaagaag tgatattctt
    47281 atgaattatg ttaataatga ttattactat actgatgtgt tgcatgacaa ctcagatatg
    47341 tttttttatt tggatttgat aagtgaatct gatgaatata ttgatcatat tatggtttat
    47401 gattattctt ttgaatatct cataacaaac aagaattatt atgctgcata tatggatgta
    47461 agcatgtttc catggtttgt aaagaatgtc tcacaaatca aaccgataag tgaactagat
    47521 aattactgta cttggagtct gtacaaagaa tatgttgcta ttacatatcg ggcagacatg
    47581 gtaaataaat ctcttacaag acgtaccttt gttactattt taattaataa tgaaaaattc
    47641 aaccgccttt taagcgggct tgcccttaca gataattcga aaaacgcatt tgcagtaaaa
    47701 aatgatatta tgctatattt tgatggtgaa agtgatctta cttcgaccat taaacatctg
    47761 gtctttgaga atttcaaaga tttgaatgaa ggtgaaagag tgatgttcat tcatgattat
    47821 caatggtatt accttcagta caggccgcta ggtactagtt atgaagcagg tattgtttat
    47881 cttattccag atagtgatct tattacaaat gaatatgaat ttatgataat atcttcttta
    47941 gtaatatcgt tgattgttct tttgcttgga tatgtaattt tcagactggt tttgaattca
    48001 aatatacaga aaccaataaa tcagcttata aaaggaatgg ataaagtcgg aaatggagat
    48061 tttacatata aacttgatga aagtatatgt aaagataatg atatgcgagg tctttttgca
    48121 gcatacaact caatgacaga cagggttact gttttaatag aagaacttta tcaggcagaa
    48181 ctgtatagaa aacaattgga attacaggca cttcaggata aaatagatcc tcacttttta
    48241 tacaatactc tagatactat aaactggacg gcaaaagaac acaatgttga tgagatatca
    48301 agaatagtta ttgctttatc tacaatgtac aggaagatat ttaacagagg aaaggatctt
    48361 acaacgataa aagatgcgct tgaaggtata gaatgctatc ttgagattca gcgtataaga
    48421 tatggagaat cattttccta tgatatatat tgtcctgaag aacttgaaaa gtgtattata
    48481 ctgaatctga taattcagac catagttgaa aattccatag tgcatggcct ggaagaccgt
    48541 tcttccggaa aactggagat tcgtgttgat aaagaaaaag atgatataat aataaaagta
    48601 attgataatg gcattgggat gactgaagaa aagctcaatc taattaactc aagtataaat
    48661 tccatgaaac tggaaagcga gtcaggtttg aggaatgttc aaaagaggat aaggctttac
    48721 tatggcatac agtatggtat taccatagaa agtaaataca aagaaggtac tacagtaatt
    48781 cttcatgtac cttataggga gaaagaagaa gatgattgac ataataatag tagatgatga
    48841 aaaatccata agagaaggta tagctgtatc aataccatgg gaaaaatatg atatcaacat
    48901 ttgcggtgtt gcatcaggcg tatctgaagc tctggatttg attgaaaaat atatgccaaa
    48961 tatagttata tcagacatat caatgcctga gatgaatgga ttggatctgc tggaaatagt
    49021 taacaggaca tatccaaata tcaaagttat attaattagc ggatataggg actttgaata
    49081 tgctcaaaga gcagtaacaa acaacgcatt ctgttatctt acaaaacctc ttgtagcaga
    49141 aacccttata tcaaaagttc ttgaagcaaa acaggaaata gatgccaagc ttaaagaagt
    49201 taagcttaat gataatatcc gaagaaaatt aagagaaaac ataatggttc ttaaggataa
    49261 tttcttccgt atgcttatgg aaggaagaat acgtcagaaa actgagatta tggaaagagc
    49321 aacaacaaca gaactgaata tggattactc aaattttatt gtctgtgttg ctgaatttga
    49381 attagctgca tctcagaaga agctcaatat gtatgacttt tctttttata aagcagcaat
    49441 aatgagtact atagaagagc atctggaaga tatatacaag tgttatacat ttaatcttga
    49501 taacagaata ggtatactgg tttgttctaa tcagcagatt cagagaaata ttttaataaa
    49561 caaacttaat aacgtaaaaa gctgggtaaa taaaaatatg ggtttttctc tttctgcggg
    49621 agtaggtaat acctgcggaa gtgttgaaaa actgactttg tcatatattt ctgcagtaga
    49681 ctctatgctg tatagagtaa tattagggaa aaacattatt gttgacgctg agcagaaaat
    49741 aagcgcttct aaagaaagaa tggctgttga tgattttgat aatatactta gaaatagtga
    49801 agaggacatg gtttttgctc taagaaattt aaacagtact gagattaaaa atattataga
    49861 ccagattatg caggcatcaa aacagatagt ttcaaatgac gtgaagcaaa aagagagaat
    49921 agctttcctt ctatctgttt ttataatacg agttatgtat tcattagaaa tgtcaaatca
    49981 caggttctat aataaagaaa acgatttgta taattctcta aatgaattga tatctataga
    50041 tgatatgaaa aatttcttaa tgggaataat taaagagatg atacagacca tagaagaact
    50101 ggacagtaag caaaataact ttttagttaa taaaggatta agatatataa aagaaggggt
    50161 atatgaagat gtatcattag tgactattgc tgaaaggctt cagatacatc caaactatct
    50221 aagtaagatt tttaagcagg ttacagagga atcttttact gagcatgtaa taaggtacaa
    50281 aatgaatgaa gcaaaaaaac ttttaaaaac aacaaatgcc aaggtttatg aaatatcaga
    50341 taaattagga tacaaagatg tcgctcattt tacaaaactt tttaaaaagc attttggaat
    50401 ttcaccgaca gagtatcgtc agttactgta attaatccat tactcagagg tatttatgaa
    50461 gaaatatatg atcagatgtg atatggaagg aatcagcggt attgtcagtt atgaacaagc
    50521 tgatccgaaa aagtccgagt atcagtatgg aaagaaacta ttcatgagtg atttgattgc
    50581 ggctgtaagc ggattaattg caggtggggc agatgaagtg cacatatatg atgagcattt
    50641 ttttggtaga aacatagact tatcagaaat ttctagcatt tcatcatcag acaaaatctt
    50701 tgtgtatatg ggaaagcctc catatgagga agactgggct ggtggtctta catctgattt
    50761 cgacggttta gtcttattag gcttccattc taaaaaaggt acaaaaaatg ctcttttgaa
    50821 tcatacttat gaaaacgata ttgaggatat attagtaaat ggtatatcag ttggagaaat
    50881 aggtattgaa actatgatag caaagcagat gtctgttcct cttatcatga taacagcaga
    50941 cagtgagggt gtaaaagaag cattgcagtt agagccagag gtaatagctg tttcagtcaa
    51001 agaatcattg ggtgaaaatt cagccatatg ttatccttcg aatagaacca gtcaggtgat
    51061 ttatgataaa gcaaaagaag ctgcccaaag ggaatggaac agacctgttg aagattataa
    51121 aactggtgtt cacatgaaaa tcagattagc agatactata tattcaaaag agtatttaaa
    51181 aatgttctcg tttccggagt ttcgtgaaaa aactgttctg tgctgctggg ctaaatattg
    51241 gcagaacaaa cttaaagtaa tggaaaaatt atgaaagaag atattacaca atacgcaaaa
    51301 ttaggacttg tacatcatat gctgtttcct aaaagtattg aagacgagac atttcatgct
    51361 gacagtttaa tagagttatc aaaacgaaat gatatcgaaa catttgattg ttgtctgcca
    51421 tatgataaat caaatagaaa tcgtgtaata gagcaattga gcaactgtag caaggaagtc
    51481 tgttatgctc ttcatctttt tccaagcaga aaaatctcat tatgttcttt ggataagatt
    51541 gaacaaagta taactaggct tgtggttgca gatcaaattg aagcagcttc agcaattaat
    51601 gccaaaggat ttgtttttgt ttcagggtct gatgtcccaa aaaacagaga ctgtgcaaaa
    51661 acagcatttt tggattttac taaatggttt gcaaatgaat taagaaaaaa aggaattacc
    51721 gcattactag aaccatttga cagaactgtg gataagaagt atctttacgg tcctattgat
    51781 gagtgtattt cattgattga tgaagtatca ctgcattatg ataatattgg aatagagctt
    51841 gatatggcac atcttccgct catgggagaa gattttgaat catctataag aagatgcggt
    51901 cacagaatta aaagagtgca tcttggtaac tgcgtattaa atgatgctaa cagcacatgg
    51961 tatggtgaca agcatccacc catatgtttt gaaaatggta tgattgctga atctgaattg
    52021 agaataatat tagaaacatt gttggatatc gagtaccttt caaaagctaa caggggttca
    52081 ttagttatgg aaatgcagcc atatccaaat aaatcggctg aatatacaat aaaaaggaca
    52141 atggaaattt taaataaagt ttgggagcag atatagatac tccttttatt aaagtgtaat
    52201 ataactggtt tatttaaatt acgcttatgt tttgtttttg aaaagggtat catttatatg
    52261 aattaagaag taaaatgtat taatagggaa cagacatatt gcacactttt agcaatgact
    52321 gaaaggaaat tgataagcca gctatttctt gcaaagggta tacccctatg atataatcac
    52381 aatgttttaa tggaggaaat atgaattttt tcagtaattc attagtatat tttataaaaa
    52441 agcctttaat accgatttat gtagcaagtg taactttagt ttactgcata atagcaatat
    52501 tcaacccgtt aaaggtgtta accaaaagct atgcatcctt tataactgag gattttggat
    52561 ctactatagt tttattgtca aaagaaatat atagttatac caatattcca tatatagtat
    52621 tgggtattct aggtatagct attgttttgg ctctattgtc tagtttgctg ttcagtgggt
    52681 atatgaatat tattcacttg acggtaaaga ggattaagac agatttctct gattatcttc
    52741 aaggcataaa aaagggtttc ttcagatgtg ctttagtatt ttttcaattg tatatctcaa
    52801 ttctgctttt tgtaggattt ttaccgctgg ctcttactcc gtttttcatt ttaagagatt
    52861 ctgttcttga atcgggtaat gatatgacta tactatatat tctgcttggt gtaattatat
    52921 ttatatgtat tactattgta cttttgatat acatgagttt tatgttcaaa ttcccatccg
    52981 tatttcattt ttccaaatat cctatagaaa agtcaaatgc agctgttaat gcaatgtact
    53041 ggagaacctt tgggaaagta actcttctac ttacgttcct agcaggtata gtatttgtta
    53101 tgtttaagat agagaaccga gtactggaat ttttaattgg atttattctc tatactgtat
    53161 atttcagttt ctttgtggta ttcccttttt atacttttga caaattgata gaaccatata
    53221 ggattagtaa ttagaataaa ttaaagtagt taattcagga ggcatatact atgtatgatg
    53281 ttgttattat tggcaaagga cctgcaggag ctcaggcagg catttatctc ggtagagcag
    53341 ggtttaagac tcttattgta tataaagatg gtggatctct ttcaaaaaca gacttcatag
    53401 aaaattatta tggctatgct gaagctattt ctgctgaaaa acttcttaat gaaggagttt
    53461 tgcaggctaa gcgtttcaat gtagattttg ttgaagatga aatagtgatg ctccagacgg
    53521 atgaggagaa taaagttctg gtaaaaggtt tgaagaagga ttatacagct tcttatgcca
    53581 taatagcagc agggatgact agaaagagtt taaatgttcc tggattgaaa gattttgaag
    53641 gaaaagggat tagttactgt gtaacctgtg atggattctt ttttagaaac agaaaagtag
    53701 ctatcatagg gtatacgaaa tttgctgaac atgagttaaa tgatcttttg atgtttacaa
    53761 aacaggcttt tcttattaca aacggaaaag aattggaatt tgatgcatct gtatataagg
    53821 aagttactgt catagacaag cctattgctt caatctatgg ggaagataca attaagggta
    53881 tatcttttaa agatggatca aatgaggact ttgatgggat attcgtagct tatggaacag
    53941 cagatgcttc ttctttctgt gtgaaatcag gaatagctac agaaaagagc cgtattatag
    54001 tagatgagaa cctaatgact aatattgacc ggatttatgc agctggagat tgcataggct
    54061 catatatgca ggttgcagct gctgtggcag atggtgcaat tgcagctcgg tctgtaacag
    54121 aaagactgaa agcagacaga aagacagcaa gaaaaaagga ttagtttaca caactactta
    54181 attagagtat actaaaaaga aattttatcg gaggaaccag tatgaataaa gaaaaattct
    54241 acataacaac tgcaatagca tatgcatcag gcaaaccgca cataggtaat acctatgaga
    54301 ttatactagc ggatagcatt gcaagattta tgcgaatgca ggggtatgat gttttcttta
    54361 tgactggaac tgatgagcat ggacagaaaa ttgaagataa agcaaagcag agcaatatgt
    54421 ctgctaagca atttgtggat aaatcagcaa aagagattaa aagaatctgg gatcttatga
    54481 atacaagtta tgataaattc atcagaacaa cagatgcgga ccatgtagtg cagattcaga
    54541 aaatttttaa aaaactttat gataaaggcg atatatataa aagcgaatat gaaggatggt
    54601 actgtacccc ttgcgaatca ttctggactg agtctcagat agaagatggg aaatgtcctg
    54661 attgcggtag agatgtaaaa aaagctaaag aagaagcata cttcttcaag ctttccaaat
    54721 atcaggataa acttatggag tatattgaga aaaatcctga ttttatacag cctgaatctc
    54781 gtaaaaatga aatgataaat aattttttga agccaggatt gcaggattta tgtgtatcaa
    54841 gaacatcatt tgaatgggga attaaagttc cttttgatcc taatcatata gtttatgtct
    54901 ggatagatgc tctttcaaac tatataacag gagtaggata tgatgctgat ggaaatagca
    54961 gcgagatata taaaaagtac tggccttgtg atttacatgt aataggaaaa gatattgtaa
    55021 gattccatac tatatactgg ccgataatgt taatggctct tgatattccg cttccaaaga
    55081 aggtactggg gcatccatgg cttctgtttg gagaaggcag caaaatgagc aaatcaaaag
    55141 gcaatgttct atatgctgat gatttagcag aactattcgg tgttgatgaa gttcgttatt
    55201 atgtacttag cgagatgaat tatgcaaatg acggtataat ctcatacaaa cagattatag
    55261 acaaggtaaa tgctgattta gctaatgtat tgggtaatct ggttaacagg acaattacaa
    55321 tgcagcataa gtattttaac ggaatagtta agaaaccgga atcactctta ccacaaaacc
    55381 agtttgatga agaaatgata gagttagcaa agcagacact aaacaaagtt actgaactga
    55441 tgaagcagta taaggtcagt gatgcgatat atgaggtgat ggcattagca aaacgttgca
    55501 ataaatacat tgacgaaaca ttaccatgga cattattcaa gaatccagat gatcataaac
    55561 gtttggagta tgtaatgtat aaccttttag aaggaattcg taccgtcgca gtgcttcttt
    55621 atccatttat accatcaact gctgagaaga tattagctca acttaacacg aaaaagactg
    55681 atattaactc attagaagaa tatgggcagc ttgaagacca gataatgctt aaccagcctg
    55741 aaatactttt caacagaata gatagtgaca agaaactaaa ggaaattgag gaatctgtaa
    55801 aagaagatca agaagatgct aatgtagtgc agatcggaat agaagatttt gcgaaagttg
    55861 agttgaaaac cgcaaagatt ataaaagctg aaccgattaa aggagcaaag aaattattaa
    55921 agcttcaggt tgatttaaac ggcaaaacca gacagattgt ttccgggata gcccaatact
    55981 atgaatgtga agatttagta ggaaaaaccg ttatagtgat aacaaactta aaaccagcaa
    56041 tgttaagagg tgaattaagc gagggtatgc ttttagcggc aagtaatagt gataagcttt
    56101 cattagttac tattgataag gaaatgccat caggttgcgg tgtttcataa aataagttaa
    56161 atgttatatt atttaaatag aaacaaggac tgtacattta caatttgtac agtccttttg
    56221 gataggctac acttaattgg acagaaaaaa taagggtatt gaaaatggta ta
//
LOCUS       DAOSXM010000005        53488 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2111
            NODE_391_length_53488_cov_11.217768, whole genome shotgun sequence.
ACCESSION   DAOSXM010000005 DAOSXM010000000
VERSION     DAOSXM010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864178
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 53488)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 53488)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:24:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,261
            CDSs (total)                      :: 2,229
            Genes (coding)                    :: 2,218
            CDSs (with protein)               :: 2,218
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 3 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53488
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_391_length_53488_cov_11.217768"
                     /isolate="AMR_MDS_2111"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            61..1239
                     /locus_tag="PLQ68_01525"
     CDS             61..1239
                     /locus_tag="PLQ68_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005886180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HXK71358.1"
                     /translation="MRYTGVTSRGIITPIFKQGDDLVSLVSDSIVKAAENENFTISDR
                     DVIGVTEAVVARTQGNYATCEQIAKDIKSKLGGKDMGIVFPILSRNRFSILLKAIAMS
                     CEKLYIQLSYPSDEVGNALIALDELDELGVNPYTDSFTESEFRKLFKNTVHVFTKVDY
                     IEAYKSMGKDIEIVFSNDPTHILKYTKNVLNCDIHSRKRTQRLIKSAGAEKSYRLDEI
                     LTSSIDGSGFNKDYGLLGSNKATEDKVKLFPRDAETFVETLQKRMLELTGKHLEVLVY
                     GDGCFKDPVGGIWELADPVVSPGFTNGLHGTPNELKMKYFADNEFASLRGEQLALAMK
                     ERIKAKDKDLSGNMESQGTTPRRLTDLLGSLCDLTSGSGDRGTPVVLIQGYFTNFASD
                     "
     gene            1239..1880
                     /locus_tag="PLQ68_01530"
     CDS             1239..1880
                     /locus_tag="PLQ68_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012175064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HXK71359.1"
                     /translation="MTYKFKEITDTVKKIFINNKGSHDWDHTERVLNMAEKIALSENA
                     DIDIVRYAAVLHDISRHDEDMSKGIIHHEEKGAIEARKVLSSFGIDDITADKVCHCIL
                     THRFRRGNKPETLEAKVLFDADKLDGIGAIGIGRAFVFAGENKARVHDPDVDVEKTKS
                     YTKEDTAYREYLVKLSKIKDRMLTKSGKEIALNRHQFMVEFFDRLNAECKGIV"
     gene            1960..3723
                     /locus_tag="PLQ68_01535"
     CDS             1960..3723
                     /locus_tag="PLQ68_01535"
                     /inference="COORDINATES: protein motif:HMM:NF027787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5050 domain-containing protein"
                     /protein_id="HXK71360.1"
                     /translation="MNRDNFIKQTTEQLTKMNVPYKVQDGTDISISATFKNKDDDSED
                     ILYTAFFFLNPADNKVYAYQKALAGQNDVSNDPSFVNMNVKRNDDDSLVPIGLILSSI
                     AGIAASLGFVFQRTDNQQMAVYPKGYIPASVGAAAQGTAFCTSCGAKMPIGTRFCTNC
                     GADSQAPAVTQPKPFVSNDAPPVYANVNQNPLPSYTQPNMQNMKPRRKTPVVIVVIAV
                     VLIIGALVTGGILLRNWIVNRKPSDNVNVPNASENALSGTNPTPTGSSSSETGSSVVS
                     AGVIHSDLGNIMNGQYYFATDEYIFYSSYDADFKAHIYSVKKDGTEHKVIFNGFGWSL
                     VVIDDWLYFSGNQGDVIDGTYNIFRMRLDGSSVERINNTYSYGMFLYDEYLYYMKMNT
                     DYTDTFSICRSSLDGSNEEILFPYGSNPVVYDDLLYYFDNQGNMFRTKPDGLDPEVLI
                     TGEVGFYVLSNDKLIYKDFSSNIYTCDLDGGNKKLVRSSSGKSILTVNSYNERIYFVE
                     YNPEFNYSTYSWDYDAISMKMDGSDEKVIFSSSSYGMYMNIVDGKLMMMDYLMDPQTA
                     IYYTYINIMNLDGTNLRVLER"
     gene            3777..4286
                     /locus_tag="PLQ68_01540"
     CDS             3777..4286
                     /locus_tag="PLQ68_01540"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HXK71361.1"
                     /translation="MPGIFSFVENINVLQAVLLVIGLIFLIIEAFVPSFGLFGVLGIV
                     LLLLSIILTATTFVEGLVMFLIILIIAVVLLIIAIRLATKGKLSKKLINRDTFSDKEG
                     YFGIQDWSASVGLKGKSITILRPAGKGEFEGNTLDVVTKGEFIEKDKDIIIEKVEGAR
                     IVVKENKGE"
     gene            4290..5291
                     /gene="floA"
                     /locus_tag="PLQ68_01545"
     CDS             4290..5291
                     /gene="floA"
                     /locus_tag="PLQ68_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254616.1"
                     /note="flotillin-like protein involved in membrane lipid
                     rafts; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin-like protein FloA"
                     /protein_id="HXK71362.1"
                     /translation="MDPIVLVLVIIAAVIAFFAILLSLVPIGLWISAIAAGVKVGLMS
                     LIGMKFRRVEPAKIVNPLIKSTKAGLNVTINQLETHYLAGGNVNKVADALIAAQKARI
                     DLTFETAAAIDLAGRDVLEAVKMSVNPKVIETPIIAAVAKDGIEVKAKSKITVRANIA
                     RLIGGAGESTIIARVGEGIVTSVGSAESYKEVLENPDLISKTVLKKGLDAGTAFEILS
                     IDIADVDVGRNIGAQLQTDQAEADKKIAQAKAEERRVMAVAREQEMKANVQEMRAKVV
                     EAEAMVPRAMAKALEEGNLGVLDYYNMKNVIADTQMRESISETGKSSKTQNDKKEIK"
     gene            5291..5482
                     /locus_tag="PLQ68_01550"
     CDS             5291..5482
                     /locus_tag="PLQ68_01550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK71363.1"
                     /translation="MSRYLKKDDDMSKVIEEFKPSIVEKKLEEKDDYDLISDLLDDSE
                     VLVKGIILSEILGKPKAKR"
     gene            complement(5562..7388)
                     /gene="glmS"
                     /locus_tag="PLQ68_01555"
     CDS             complement(5562..7388)
                     /gene="glmS"
                     /locus_tag="PLQ68_01555"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015310950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HXK71364.1"
                     /translation="MCGIVGYVGNENAAPILIECLKKLEYRGYDSAGIAVIDKNEINM
                     VKATGKIKNLCDKTNDGCSLSGNIGIAHTRWATHGAPNDINAHPHVSNNKKFAVVHNG
                     IIENYMDLKKELVDKGYKFNSETDTEVVVHLLDMYYDKDFKKSVMKTVSRLEGSYALG
                     VICKDYPNTIYAAMLNSPLIIGVGVSENYFASDVTALVQHTKNVIYIEEGEFAEITSE
                     KINVFDCTGEPVAREITRIVWDVEAAEKGGYEHFMLKEIMEQPRAIKSAIEPRIKKGK
                     ITLDDSGMPDDIMKDINRIVITACGSAYHAACTGKYMIEQLARIPVEVDLASEFRYRN
                     PIINKSTLVIVISQSGETADTIAALKEAKQLGAPVLAIVNVVGSAISKLADYVIYTWA
                     GPEIAVATTKGYTTQVTVLCLLSVFLAQKVGSIDKKTASSLTEDIQQIPKLAQRAIDL
                     NPQIPYLARKYHNERSMFFIGRNTDYAVCLEGSLKLKEISYIHSEAYAAGELKHGTIA
                     LIDNDRLVVALACNERLLDKLVSNIREVKARGAKVLGIITERSTKIIPESDDIIYIPE
                     GNTLFNAVAQVIPLQLFAYYVALENDCDIDKPKNLAKSVTVE"
     gene            7581..>8416
                     /locus_tag="PLQ68_01560"
     CDS             7581..>8416
                     /locus_tag="PLQ68_01560"
                     /inference="COORDINATES: protein motif:HMM:NF025001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HXK71365.1"
                     /translation="MKKIRLKIDIADSEYSDRLSEYIKIKGLNIFEITDFNQHITLSD
                     LRGEDSDCLHLLSEKEDVLISKYQSADRIIQSVMENVSKCNEETVKIVSRNCRVIFVT
                     SGHGGAGKSCISQACAIYWARKGKKVMYINVDGFSVNESIFNTEKPYDISLLMHYTNK
                     VNSDFNVYIERYKNHDSEHDVCFLSSVYPSCDTCFNYEQGQYFMNSLISNDIYDYIFI
                     DCPLYANKTYIDMMKLANKTIFIKNKGSKREEEVIRFLENNGISLLIACNMVRYTEGV
                     YIP"
     assembly_gap    8417..8516
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8517..9536
                     /locus_tag="PLQ68_01565"
     CDS             <8517..9536
                     /locus_tag="PLQ68_01565"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HXK71366.1"
                     /translation="ILDSIIGYGFIQPLMTRKGITEIMINSDKIFFEEKGRLHRYNKN
                     FTSQDVERLINILCRKTDRTVNMSCPILDGSIGHDIRVNIVLSPIANNGDSITIRRFP
                     QTPITDEDMVNEGFFDKEIRDFLSYAVKNRYNIFICGGAGCGKTTLLNVMSTFIPKDE
                     RVITIEDSVELMMNKIDNVIRLICRNPNSEGKGKIDIGDLIKSSLRMRPDRIIVGEVR
                     GGETLYMLQAMNTGHIGSISTGHANSCKDAFSRLETMVLMECDMPLLSIRKQICNALD
                     IIICLGRINGKRTVLEISEVLGFEGNEVKLNMLYIYDFNKKYHEKVNSVIRTEKMDRG
                     MYNEI"
     gene            9526..10236
                     /locus_tag="PLQ68_01570"
     CDS             9526..10236
                     /locus_tag="PLQ68_01570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK71367.1"
                     /translation="MRYEFIKHAITVGFAYGFAAYCLYLSQLVAISVFLIVMVICLLN
                     KKQMDMLISKSMRDSFERFLEILASYVAIPGNFMHAFTKALAEHEKIYPKDILNCAAK
                     KAYIKCSANIPSDIILTSFAKDINIQEGYLFCESLVICEKKGGDINRTIRETIAFIKE
                     KTGIEHEIETITAEKRIERIIISICPFGILALLNSMGTGYLDVLYEGLLGRTIMTIAG
                     VLFLISSYIAKKILEVRI"
     gene            10401..11054
                     /locus_tag="PLQ68_01575"
     CDS             10401..11054
                     /locus_tag="PLQ68_01575"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HXK71368.1"
                     /translation="MFLIYPEKSVIDITIVHISMKICMFFIMLFLPMVAFAVNASTYL
                     KVISFVMCIMSVKCIDLDIKSEYELLKEKILSDFSIYLTNITLYIGSGMTLYQAFVQQ
                     DINMPEGILKDQMKKLIKSIELRSSPEDAFLSLSIALPITQINSFCSIMISGFKNTES
                     GLKDTLKAYTSSIWTEKKNRAKIRAEQAGAKAVFALAIGLVGILLILIAPAMLMLNS"
     gene            11070..11243
                     /locus_tag="PLQ68_01580"
     CDS             11070..11243
                     /locus_tag="PLQ68_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010248075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp1 family type IVb pilin"
                     /protein_id="HXK71369.1"
                     /translation="MYNRLNKKGMGTVEVVIILAVLVGIALIFREAITDFIQKIIDGT
                     LNDETIIERLRIN"
     gene            11246..12073
                     /locus_tag="PLQ68_01585"
     CDS             11246..12073
                     /locus_tag="PLQ68_01585"
                     /inference="COORDINATES: protein motif:HMM:NF039422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6382 domain-containing protein"
                     /protein_id="HXK71370.1"
                     /translation="MISEIKSGNSQVLIRVMGQNMECAPYMIKMLEKTECNLLLPIKY
                     ATDEEQLYDTVYGLSLDVYMKKYGYTPDLLKNLMYSIQSLLRVSESYLIDTDHIVLDT
                     QYIFVYDNSNFKYIFDPYNKQNTNENIKKLVNSIVSGNFANKALTEAMNQQSFSLKLV
                     IDSLSDEKDEKMIGGLEEDEAVILRNIIDADDCIKVSKKDVYVGKLPLINDKRLIGKL
                     SSFRHGCIRYSRKSVYIKDLNSKYGIYVNGERIKSNTFFSIHKGDIVAFGDDEYILL"
     gene            12147..13235
                     /gene="upp"
                     /locus_tag="PLQ68_01590"
     CDS             12147..13235
                     /gene="upp"
                     /locus_tag="PLQ68_01590"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008520964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HXK71371.1"
                     /translation="MIAIGCDHAGFKLKSEILSYLDKKKISYRDFGAYSDDSCHFPFF
                     ASLVATAVQKNECERGILICGTGIGMSIAANKFKGIRAALCMDEYQARMAKRHNNANI
                     LVLAGRITPKEKAQDIIDIWLNENYLEGKYETRNQLIRRIEEGGSMSGTNYKDMAFVF
                     DHPLIQHKISIMRDKNTNTKEFRELVKEVSTLMGYEVTRELPLEDIEVETPIAKTTVK
                     VLSGKNVGFVPILRAGLGMVDGLLTLIPTAKIGHIGLYRDHDTLEPVEYYCKLPEDAH
                     ERMIILLDPMLATGGSASAAIGFLKQRGVKNIKLMSIIAAPEGIEKVHKDHPDVNIYC
                     ACVDKELNTNAYIVPGLGDAGDRLFGTK"
     gene            13235..13681
                     /locus_tag="PLQ68_01595"
     CDS             13235..13681
                     /locus_tag="PLQ68_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006306262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HXK71372.1"
                     /translation="MDRPSWDEYFMQVVHLVKTRSTCLRRKVGAVIVKDKRILATGYN
                     GAPIGCRHCEEVGCLRQKLNVPSGERHELCRAIHAEQNAIVQAARAGTSIDGATIYVS
                     AQPCVICAKMLINSGIKRIVFEGDYPDELSKELLDESGLELVKYGG"
     gene            13683..14801
                     /locus_tag="PLQ68_01600"
     CDS             13683..14801
                     /locus_tag="PLQ68_01600"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HXK71373.1"
                     /translation="MSYLYTFLASFIIAFALSPLVIRFAKAKKILKYPKRNRDVHTEP
                     VPLLGGTAIIISFSISILLNYLINPNFYVTIEVIGLAVGIVIISFMGILDDIYDLKPV
                     IKAGFQFLAAIATVLISGSKIAYFTNLGQNNNLVYLPEIISFIATVLWIFMLTNAFNI
                     IDGLDGLTTGTTIICSLTLFFITFVRPDANLVGEYIPILLIALSGAAAGFLPFNFNPA
                     KLYLGETGAAFLGFTVAVISIQGTMKAHAAIAVFIPLIAMGLPILDISLAYFRRIIMK
                     KPIAVGDRDHIHHRLMKMGLSQRKTVLILYFINIVFGVTAYLLSGQQYSRFWIYFAIV
                     LILLGLAVYVLYGPLLKQAAKNGNGNSVTQEKEESEDD"
     gene            14794..15912
                     /gene="wecB"
                     /locus_tag="PLQ68_01605"
     CDS             14794..15912
                     /gene="wecB"
                     /locus_tag="PLQ68_01605"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HXK71374.1"
                     /translation="MIKVISVFGTRPDTIKMAPLVALLDKDESFEHIVCVTGQHREMV
                     KQILEVFDINPKYDLDIMKQGQSLDYVTSAILVRFSEVLDIEKPDMILVHGDTNTCLS
                     ASIAAFYKKIPIGHVEAGLRTEDIFSPYPEEFCRRTVDSIATLYFAPTKSNANNLINE
                     KIDTDRIFITGNTVIDTLKTTVKENYIFNEPMLNDIDYKNKKVIPITAHRRENIGINL
                     ENICNAVKTLAKEYKDSHFIYTVHPNPKVRTTAEKILSGIGNVTLLPPLVFTDLHNLM
                     ARAYFVMTDSGGLQEEAPSLGVPVLVLRKETERPEAVEAGTVKLTGVKLNDILTDAKM
                     LFDDQTFYAKMKNAVNPYGDGNASQRIVDSIKAYFNNL"
     gene            16044..17117
                     /gene="prfA"
                     /locus_tag="PLQ68_01610"
     CDS             16044..17117
                     /gene="prfA"
                     /locus_tag="PLQ68_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004625578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HXK71375.1"
                     /translation="MQDKLRNIERRYEEINAQLSDPDILANQEKYVALMKELNEISEI
                     VKVFREYTETLTGIEDVYLMLKENSDDPDFKTMLEEELEMFEEKKPVLEQKLKVLLLP
                     TDPNDNKNVIIEIRGGAGGDEAALFAANLLRMYTMYAESKKWKTEVLDLNEIGIGGIK
                     EVVFSINGNGAYSRFKYESGVHRVQRVPETESQGRIHTSTVTVAVLPEAEEVDVDLNL
                     NDVQVDTYRSSGAGGQHVNKTDSAIRLTHLPTGIVVACQDERSQHKNKDKAFRILRSK
                     LYDLAQENQDKEIAQNRKSQVGTGDRSERIRTYNFPQGRVTDHRIGLTLYKIDSIMNG
                     DLDELMDALITEDQARKLSNQDE"
     gene            17110..18126
                     /locus_tag="PLQ68_01615"
     CDS             17110..18126
                     /locus_tag="PLQ68_01615"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002582299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HXK71376.1"
                     /translation="MNNIKTRLFNTVNEESLKESADIIKGGGIVAFPTETVYGLGADA
                     LNPNAIEKIFLAKGRPNDNPLIIHIHNISVLDKYVKEIPSVLTMLSKLYMPGPLTVVL
                     KKKDIIPNNVTAGLDTVAVRIPDNEIALKLIEYSNTPIAAPSANISGRPSPTMYEHVV
                     SDLFGKADAIINGGKTRIGLESTVLDLTTEIPVILRPGGVSYESLKLILTDVETAYSF
                     DTEKPKSPGMKYKHYAPIADMVIVKGKTDDVVRKINEISDDDCAVLCCKETYERYISN
                     NKLSAGSINDPSEIASNIFQCLRLFDQMKIKKIFCEYFEMKDIGEAVMNRLLKAASQN
                     IIKV"
     gene            18128..18421
                     /locus_tag="PLQ68_01620"
     CDS             18128..18421
                     /locus_tag="PLQ68_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007490331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK71377.1"
                     /translation="MSQILEACMVICFGISWPMNIIKSLRSRTAKGKSLLFLILVLLG
                     YCCAITGKIISDSVNWVFWFYVVNLVMVITDIVLYYINRKADIEADGYTHNRK"
     gene            18396..19103
                     /locus_tag="PLQ68_01625"
     CDS             18396..19103
                     /locus_tag="PLQ68_01625"
                     /inference="COORDINATES: protein motif:HMM:NF027552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4886 domain-containing protein"
                     /protein_id="HXK71378.1"
                     /translation="MDILTIGNSFSQDITTYLQRISNKDIYVRNIYIPGCSLEQHANN
                     ILNDNKTYEYQKDGRRLYMTNLKEALYKRQWDIITLQQVSHLSGIESSYHPFLVALIQ
                     YIEEKCPNAKLMFIKTWPYEKGCSHSGFQNYNNDTYTMYKSITNVVENTSKRYNLEII
                     DVGNTIYNAYIKTSNGSDIEKIEYYRDGFHLSIPQGRYLAALAFHNHLYGKDGINYKY
                     KPKKMTKIQQEILLNHI"
     gene            19166..19430
                     /gene="ffs"
                     /locus_tag="PLQ68_01630"
     ncRNA           19166..19430
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PLQ68_01630"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
     gene            19449..20981
                     /gene="dnaX"
                     /locus_tag="PLQ68_01635"
     CDS             19449..20981
                     /gene="dnaX"
                     /locus_tag="PLQ68_01635"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HXK71379.1"
                     /translation="MAHIALYREWRPTSFEKVVEQEHITKTLKRAVVTGRISHAYLFS
                     GTRGTGKTSLAKIFARAVNCLDPKDGEPCNKCEICKKALDETLMDIAEIDAASNNSVD
                     NIRRICDEVNYMPSLAKFKVYIIDEVHMLSTSAFNALLKTLEEPPSYAIFILCTTEPN
                     KLPATVLSRCQRFDFHRITVKGIEQRLKLIAKSENENITDDATEMIARISFGAMRDAI
                     SLLDQCISSTDKTIEVDDVLNCAGLIDEKSNAKVSKALAENDVKGILSSVNDVIAEGK
                     SPSRFLSGLLGYLRNILVCMNINDPSDFIEADSNAIEQMKTLSKMFDADTVIRYIKEL
                     SENEAVLRRASQPKTYLEIMLIGLLVKEDVKPIEEKSETKKQAIKEIKEEKSYETEKK
                     ASDISIESSVKQPEKQSQSKAWSKFIQDRISEGSKDVFELKDSSIEIVGNQAKITVQS
                     TFLKDSLEYNKDEVLKEIQGYNSEIKNVKFVCSANNVQVNTIEGDLLKIGKEHNIEVE
                     IE"
     gene            20998..21327
                     /locus_tag="PLQ68_01640"
     CDS             20998..21327
                     /locus_tag="PLQ68_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006441197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HXK71380.1"
                     /translation="MARGMMGGNMNYNSMIKQAQKMQADMAKAQEELEESKFESTAGG
                     GAVTVTLKGDYTMESIAIDPEILEDADVEMLQDLILTAFNESVEKINAKKEETMGKAT
                     GGLRMPF"
     gene            21328..21924
                     /gene="recR"
                     /locus_tag="PLQ68_01645"
     CDS             21328..21924
                     /gene="recR"
                     /locus_tag="PLQ68_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003390192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HXK71381.1"
                     /translation="MADMYPKPLSKLIAAFEMFPGIGRKMASRLAFYLLTMDDDRIKF
                     ISDAIKDAKAGLSYCSICCNITQNDPCEICKSDRDHSVICVVSSVKDLMAMERTCEFD
                     GVYHVLHGVISPMEGVNPEDLKIKELLKRLDKGVTEVIIATNSSVEGEATAMYLSRLI
                     KPFDIKVTRIAHGISVGTEIEYADEATLIRALKGRIAI"
     gene            22214..24010
                     /locus_tag="PLQ68_01650"
     CDS             22214..24010
                     /locus_tag="PLQ68_01650"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXK71382.1"
                     /translation="MKKIGLLKQFLKGNGVAYVAGLIFMIIGQLLSLANTFIIGVVID
                     SVIGEILPEGIAYKGLSLLGGKEHLMQNLWICGLVLVLFSVINGIFMFLSRKYSAIAS
                     EGIAKKIRMDLYEHIQNSTYLYNVNIKTGDMMQRCTSDLETVRNFLATQFQDLLRCIV
                     LIIATVSIMSNINTTLTLAGFSLIPFIGLGAFFFFKYVSKMFLLADEAEGAMSTVIEE
                     NLSGMRIVRSCATQSRETEKFVKSSIDYRNKDLKITVAFAWYWGLSDAICQMQIGIVL
                     VTGIYLALRGDVTVGMLTVFMTYVTQLIWPIRGMGRVLGDMGKANVALGRIKEVLDAP
                     REPGREDAGLTPKIQGSITFENVSFGYTSDKPVLEDISFEIEKGKTLAILGRTGSGKS
                     TLLHLLVGLLPYKEGSIKVDDVELSEINKRHLRKNIGIVLQEPFLFSKTIKDNISDND
                     PSITDEDIQRATTIASVDQAIKGFENGYETMVGEKGVTLSGGQKQRVAIARTLINNCK
                     ILIFDDSLSAVDMQTDKNIRNALRKHCSDTTTILISHRINTLRQADLILVMEDGHITD
                     RGTHNELIQREGLYKQVYDMQSCVLEMEKAGD"
     gene            24013..25860
                     /locus_tag="PLQ68_01655"
     CDS             24013..25860
                     /locus_tag="PLQ68_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008908714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXK71383.1"
                     /translation="MEQDNKEIFNKSIDVKLWKKVLKYLGKYWKHVVGIVIAMVIQAA
                     TDATFPLFTEYAIDNFIANKTSEGIVKFSILFVILLLLKGLTIYIFIYLADRVWHLVS
                     HDIRVDGFKKLQELSFSYYDRNATGSLFTRITSDVGRLTGIISWGLVDMIWAISMITM
                     MTTFMFMKNVKLTLVVLTVVPILIVISIFFQKRILFYSREARRYNSIITSGFSEGIQG
                     ATTTKTLVREESHINEFEKKTDDMKNAAIKSAVYSGLFFPIVSILGIVGTSLAVWQGG
                     VMILGEIITVGTLTLFIIYTLQFFNPINDMARVFANFQSSQAAGERIMSLLEADIDIS
                     DSDEVIEKYGKITSDMQVPKLKGSIRFENVSFQYKEGEKVLDTFNLDVEPGQCIALVG
                     ETGSGKTTIVNLACRFYEPTEGVIYVDGEDYKKIPLMYIHKNLGYMLQSPHLFSGTIM
                     DNIRYGKEDATDEDVMNAAKMVEAHDFIMKFEDGYNHVISKGGGGISTGQKQLICIAR
                     AIVNDPCIFVLDEATSSVDTQTEKIVQRAISKVLSNRTSFVVAHRLSTIREADKILVI
                     DKGKVIEQGSHDELMILKGNYYNLYMNQFIEDSNKDIFTNPELTAAECQ"
     gene            25932..26957
                     /locus_tag="PLQ68_01660"
     CDS             25932..26957
                     /locus_tag="PLQ68_01660"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HXK71384.1"
                     /translation="MNENNVHEIKKESLISALNNKFDKKIFDVEYTADRLCKGTVAEV
                     IKIEGNAKNHEYILPFKLVLKIQKKWNRHLDPFCWRREYDIYLNKLDKEISRFIRLPE
                     CYLLEESHDTTKIWMEYIEGMSGEDDIDDKKLSLAAEKLGMLQADFHKSGSFDLPYIR
                     NFPAVESSFDLWYKRIEEVLKKPISGFPDKIREILNQYAENSNNLLASFKSLPLTLCQ
                     GDVHHDNLLFKPENDGFDIYLLDWDSSGYGYMGEDAIDLLLEAFLYTKRDVSFMNEYK
                     HKILSGYCNGAKKSRMDYFLDDKLITDIFAYSWGFRVTSHYLYYKTDDLKKRCVDILN
                     VMFGLKI"
     gene            complement(26954..27703)
                     /locus_tag="PLQ68_01665"
     CDS             complement(26954..27703)
                     /locus_tag="PLQ68_01665"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HXK71385.1"
                     /translation="MDNPIISHLRKPPKLKGIKRALFIQPHADDNQIGAGGMMALLAE
                     SGAEVYELTVTDDRLLERDKNGPTTRQSEALQAQQFLNVKNAGFLGFCDKTQEPVEKI
                     SQAILPIIRKIKPDAVFTPDPNLATECHSDHIKTGTAVKYAVLDAMCDFYPYSEDGKA
                     RHEIWTTPILAFYYTDKPNRKVDISKYIDKKLDSVKCHKSQVDNNLLMFVKELAKIAA
                     KGTKYKYAEQYRALSTLQTHCFNISLDADDD"
     gene            27879..28913
                     /locus_tag="PLQ68_01670"
     CDS             27879..28913
                     /locus_tag="PLQ68_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018595648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HXK71386.1"
                     /translation="MNEIYAVTGALGFLGNAIVDNLLSKGKTVRVLDIGQDKHDLLKG
                     KSVTFYKGDICLTSSLEDFFKVDDDQQLIMIHAAGIVSISSKYSQLMHDVNVKGTQNI
                     VDMALKYKIKRLVYVSSVHAIRELPKDETIIETLDFNPDKVKGEYSKSKAMATKIVLD
                     SVEKGLDAVVVHPSGIIGPGDYAGGHTTQLVKDCANGNLTACVNGGYDFVDVRDVAEG
                     VVLAAEKGIKGNTYILSGEYYPVKKITDAICSAIGRKQIKTILPLWFAKMTAPFAELY
                     YRIRKQKPLFTRYSLYTLQSNSKFNSNKAQKELGYKKKFSLKQSIEDTYLWCKANNLI
                     NQKKKAKGKV"
     gene            complement(28919..29980)
                     /locus_tag="PLQ68_01675"
     CDS             complement(28919..29980)
                     /locus_tag="PLQ68_01675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK71387.1"
                     /translation="MRLLYDKVYEHICKVDFESIWEGFHAYHFALYDDKKVYFKDKTI
                     MYEECFLGNTSIKYDDEQIAIWKIDDYSKEDPIELAANMIHEMFHAYQYELGEKRFPN
                     DILALDYPVILENVLLKHAENQLIARAVKEKDKKKIKEYISNIITSRFKRKELIKDHI
                     DYEYRIETVEGMAEYAGLLALKQLDSEKYTDKINNYFNNLADIEHILFDNRRLSYFSG
                     SLFLLSLHNTDISVFHDISTEKRTVYELISESIPSGNLPETVNTDTIETSLKTFLKNR
                     QKLFDEFFDKSPNCREGSFTICGYDPMNMVKTENLVWCKYFVMLKEKAKDNPLLINGP
                     LVIKVDKSDTFKVLAYYTI"
     gene            complement(30121..30579)
                     /locus_tag="PLQ68_01680"
     CDS             complement(30121..30579)
                     /locus_tag="PLQ68_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016298111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HXK71388.1"
                     /translation="MNTICGIDCSVCSYIKTCGGCIATHGQPFGKDCVIAKCCYDKGQ
                     NFCSECPEGSCIFQEKLIEEFNNLGIEDMEEVTKLNALKGSYVNLEYTLPSGQTIRLL
                     DDNKIYIGYQLEKKNSSRCYGIVADEKFLLVCEYGKDGTDAEIVVFKNRT"
     gene            complement(30752..30828)
                     /locus_tag="PLQ68_01685"
     tRNA            complement(30752..30828)
                     /locus_tag="PLQ68_01685"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(30792..30794),aa:Val,seq:gac)
     gene            complement(30842..31972)
                     /locus_tag="PLQ68_01690"
     CDS             complement(30842..31972)
                     /locus_tag="PLQ68_01690"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR00045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HXK71389.1"
                     /translation="MKIAVAMDSYKGCMSAITCCDIIAKALTDAMVDCDILKYPMADG
                     GEGTVDCLVFATRGRKEYITVKGVFGNEMTGYIGILGDNKTAVIESACASGIQGIPKN
                     KLDPMKASSYGTGQLIRAALEKGYRKIIVGFGGTATSDGGMGALAALGMVFFDKNNTV
                     LAPCGAEMINVDSIDSTSIIKELENAQIEFACDVENVYHGKEGAAYVFSAQKGATPEQ
                     VVELDAGLENLAKIFKKKLNKDISNVSGSGAAGGLVGGLMTVCKPVIKSGFDVILSYT
                     DLEDTIKNSDIIITGEGRTDSQTKYGKLPMRVGMLSKKYVKPVICISGSVTKDAEVLK
                     DYGINSLFSISRGPISLEDSMRDSENLLYLQTFNIAGLLGLL"
     gene            complement(32006..32974)
                     /locus_tag="PLQ68_01695"
     CDS             complement(32006..32974)
                     /locus_tag="PLQ68_01695"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HXK71390.1"
                     /translation="MNYIKIKNTNITVSKMALGTWVFSNDTTWGSQEDELSIKTTHAA
                     IDAGITLIDTAAVYGNGKSEEVLGKALKGRRDKVVIATKTADAHPDKETIIKACEDSL
                     KRLNTDYIDIYQIHWPRRTVPFCETMEGLLKLYKDGKIRAIGVSNFGVQDLNEITKYG
                     PIHTNQMAYNLLFRAIDHDIRPLCIEKEIGILCYSPLAQGLCTGKYRHIDQIPERLRR
                     VWYLSDEFSHLHSDLFKTIDCIEKIAESMNVSMSALSIAYDMHTEGIASVLVGARNFD
                     QLNQNISAAELKLDDDIMNRLIECTDNLFNMMGYNADMWRAESTIK"
     gene            complement(33006..33149)
                     /locus_tag="PLQ68_01700"
     CDS             complement(33006..33149)
                     /locus_tag="PLQ68_01700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK71391.1"
                     /translation="MHSADMLAELLKDIDSPDRYLHGIETDNAYLERYNEVMKELGME
                     MMV"
     gene            complement(33365..34576)
                     /locus_tag="PLQ68_01705"
     CDS             complement(33365..34576)
                     /locus_tag="PLQ68_01705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK71392.1"
                     /translation="MRKKLYAMITIVLLLSVMLSSCVNTGGLKILKPDNSKPDEYYSS
                     MNQGGQIAFSYLNTPYGKYQNIGGFVYYSQYGNTEFKKLCTKEDCDHSTNECDSFVGN
                     EIGYNTHIGYYNDKIYFTEFVSDMTLKLMSMDMFGKNHEVVKTIAADYDMASYISYGC
                     FHNGYYYFLVTEGGSLGAVGNKDDNFYRIKLDSKSEYEKVFRDDAISEISMFTIVGDK
                     VFFYVNKTGINPTDYYNLKSDSIDFDLYMLSLEDNKFEHMTDKWAEYTNCYYDAEKGY
                     CYKHNDGFYTLDLATKQMNKVSDSVIEGGWRSVANYYEDNIYVITFTNTKDDRGYDLF
                     SNSQTLYIFDKQYTLLDTYNGEFTGTTLGNMIDDTGDFIVISSNYKYPPDYYINKSDI
                     GEGIILHKIGE"
     gene            34764..36887
                     /locus_tag="PLQ68_01710"
     CDS             34764..36887
                     /locus_tag="PLQ68_01710"
                     /inference="COORDINATES: protein motif:HMM:NF036578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK71393.1"
                     /translation="MGRYIFKQAEPVWANRLQKEMNIRMGFMAKVGKCKASVNLACST
                     NYHIYVNEKFIASGPARAAHGYYRVDEIDITNYLIKDVNSVAVIVWGYCVNAYAVLDQ
                     PSFLTCEIICDNEVIAATGVKGFNAYLLDDYVRKTQRYTYQRAFIESYVLNTNSNDWM
                     KDLIIQQASLEICEKKEYITRNVRYPEYEKLYPLDSVVVGKHNPIKPDSYRLPWHLKI
                     EDENYKQFKTEELDENICEMMQQIEYDTSETVIKQCANGLNLSKGEYAIYEFEREATG
                     FISFKINCEETCEFLMIYDEMLSDGITNPIRIHGLWVNKYVLEPGEYDLVFFEPVSMK
                     YLNTVMLKGKASISSFHMIEYKHPHIDKSLNTSDPDLLEIFDAAVETYRQNSLDIYMD
                     CPSRERAGWLCDSFFTSRVEYFLTGKSLIEKNFLENFLLPETFDNLPKGMLPMCYPSD
                     HVNGNFIPNWAMWLVLQLYEYKQRTNDVQMIKVFKPKVYGLLEYFKGYINKDGLLQNL
                     DKWVFVEWSFANKLVQDVNFPSNMLYALMLERISALYDDSDLLVQAESIRQRIRKISF
                     NGEFFADRMVMTDNALKVTDECTEVCQYYAFFTKTATKKLYPKLWKTLIKDFGPDRVE
                     KKLHPGIYPANAFIGTYLRMELLSEQGLHKQLLSESVGFFKYMAERTGTLWENKTDNA
                     SLNHGFASHIAIWLDKAVEALQKKE"
     gene            37070..37282
                     /locus_tag="PLQ68_01715"
     CDS             37070..37282
                     /locus_tag="PLQ68_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009061436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HXK71394.1"
                     /translation="MNNGTVKWFNSEKGYGFISNDEGGEDVFVHFSAINSDGYKSLKE
                     GQKVTFDVEQDPKNSKKLRAANVCLA"
     gene            37489..38115
                     /locus_tag="PLQ68_01720"
     CDS             37489..38115
                     /locus_tag="PLQ68_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002590787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HXK71395.1"
                     /translation="MFRNKSIEEFIEVLSSKEPVPGGGGASAFAASMGTALASMVGNL
                     TLGKKKYIDVQEDIKILQEKTKVLTDELMNLIDEDAKVFLPLSKAYSLPSTTEEEKIY
                     KEQVMEEALNIASSVPVKIMEKSHQMLLLLDEYADKGSKIAISDVAVSAVMLKAAISG
                     ASVNVYINTKMMKDRTVADKLNSYCDELIQKGTKLADEIFERVEKKLR"
     gene            38120..38968
                     /locus_tag="PLQ68_01725"
     CDS             38120..38968
                     /locus_tag="PLQ68_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002590786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HXK71396.1"
                     /translation="MDILYGNDVVKSMAEQMKRQLEAIVGYIPHLAIVRVGERLDDLA
                     YERGAVKRCEKLGIRCTVYSYPSDIGNQAFEFAFSDINADDDVDGILLLRPLPRHIDE
                     LAITQMINPIKDVDCISPINIAKVFSSDESGFAPCTAEAVMEMISYAGIDLKGKRATV
                     VGRSMVIGKPVAMMLMKKNATVTVCHTRTVDMEGTCKNAEVLIAAAGRAKMINSSYVA
                     KGAYVFDVGINVDDSGNMCGDVDIDDISSLAQLCTPVPKGVGTVTTSVLANHVIKAAM
                     LRRGNV"
     gene            38965..39624
                     /locus_tag="PLQ68_01730"
     CDS             38965..39624
                     /locus_tag="PLQ68_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012200839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HXK71397.1"
                     /translation="MITFEDVKKNEEIKTYITKADESLIALGYTEHSFAHVMKVATTA
                     EYILTTLGYPQKDIELAQIACYLHDIGNLINREDHAQSSALLAFRILKDMGASPDEIA
                     TVVTAIGNHDESTAFAVNSVAAALILADKTDVRRTRVRNSDTSNFDIHDRVNYSVKKS
                     TVRINEDQTEISLSLTIDPKFYSALEYFEIFLGRMILCRKAAEKLGLKFRMNINNQQI
                     L"
     gene            complement(39730..40578)
                     /locus_tag="PLQ68_01735"
     CDS             complement(39730..40578)
                     /locus_tag="PLQ68_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ARMT1-like domain-containing protein"
                     /protein_id="HXK71398.1"
                     /translation="MKIFNECVPCLMRQAYEAAKMATDNLNIQQEILKKCAILISNFT
                     DYNSSPELAYHMHNTVKMLTCNNDPYEDIKKKDILSALKLYPYLKKILNDKNNDLYTA
                     LKISAIGNIMDSAIFKDLDVMECIDHQLDMDFTICDIEIFKKDIVKAKTLLILGDNAG
                     EAVFDRVFIESLPENLKVYYATRHAPIINDATLSDAKSAGLNNCSTLISSGCMTPGTV
                     LKDCSDEFMDIFDKADIVISKGQGNYEGLEDYSRKVYFLLKAKCDMIANRFSVPVMSY
                     IFKLSR"
     gene            40772..41185
                     /locus_tag="PLQ68_01740"
     CDS             40772..41185
                     /locus_tag="PLQ68_01740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK71399.1"
                     /translation="MKKIKVLSSFVVLVLIAVMLSSCSFTTANFSNLTMSTGVDGFKP
                     VNATTKFTTTTPAFYVTGDINNAPDDTVISAVWYYLDTDSDYMIDQSQYQVEGTNNSF
                     YFSLSIPDNGWPVGTYRVDLYIDDAVDQSIFFTVE"
     gene            41238..42170
                     /locus_tag="PLQ68_01745"
     CDS             41238..42170
                     /locus_tag="PLQ68_01745"
                     /inference="COORDINATES: protein motif:HMM:NF012354.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HXK71400.1"
                     /translation="MNNLLHLKYAVEVEKTGSISKAAHNLYLNQPQVSKAIRELETSI
                     GITIFDRTPMGVYPTKKGAEFLSYAKNILAQLEAMESLSRPESKKGQILSVSVPRASY
                     VSSAFTSFLNDLDKDSGMNIDYRETHSVRAMKSVSEGSNDLGIIRCQMRFEGYFLTAL
                     QKYALKHEDIFEFEYLLLMSKDHPLAKQKEVNYKDLLDFTEVAHGDISVPSLPSINLP
                     DKSKVFKNRIAIYERASQLEILNRIPYTYMWVSPMPKDVLDKFELVEKKCSVPKNKYK
                     DMFIYREGYVFSDYDKIFMEKVRESISKVSLMIK"
     gene            42287..43477
                     /locus_tag="PLQ68_01750"
     CDS             42287..43477
                     /locus_tag="PLQ68_01750"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002602413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HXK71401.1"
                     /translation="MARFTLPRDIYHGRDSLESLKTLEGSKAIVVTGGSSMRRAGFLD
                     KVVEYLNQAGMQVLLFEGVEPDPSVETVLRGAEAMKEFQPDWIVSIGGGSPIDAAKAM
                     WAFYEYPETTFEQLCIPFNFPKLRTKAKFCAIPSTSGTATEVTAFSVITDYEKGIKYP
                     LADFNITPDVAIVDPQIAQTMPKKLTAYTGMDALTHAIEAYVSTLNSDYTDPLALHAI
                     KMINEYLVDSYNGDITAREKMHNAQCLAGMAFSNALLGIVHSMAHKTGAAFSGGHIVH
                     GCANAMYLPKVIKFNAKNPIALERYAQIANFIGLKGRNDEKLMEALIERIRELNTVLN
                     IPMCIKDYENGIIDEKEFLDKLDNVSQLAISDACTGSNPRSIDKEQMKKLLLACYYDS
                     EVNF"
     gene            43489..44121
                     /locus_tag="PLQ68_01755"
     CDS             43489..44121
                     /locus_tag="PLQ68_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004463119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HXK71402.1"
                     /translation="MNYVSRQTLERLPVYLRFLKSQCIDGPINTSAKTIAEALSLTEI
                     QVRKDLAAISDGGKPRIGYITKDLINDLEDFLGYKDVNDAIIVGAGKLGKALMSYKGF
                     AEYGLNIVAGFDTSDEAVGTDEEGKPIFHLDRMEELCRRMNIRIGIITAPADYAQRIA
                     DKMVKSGILAIWNFAPAHLEVPDNVLVRNENMAASLAVLSKHLSEQLMNS"
     gene            44133..46748
                     /gene="adhE"
                     /locus_tag="PLQ68_01760"
                     /gene_synonym="adhC"
     CDS             44133..46748
                     /gene="adhE"
                     /locus_tag="PLQ68_01760"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015710036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HXK71403.1"
                     /translation="MNKTREIIDGTEKLNAALDRLRAAQRKFATYTQKQVDEIFLAAA
                     TAANKQRIELAKMAVEETSMGVVEDKVIKNHYASEYIYNAYRDTVTCGVVEEDRAFGI
                     KKVFEPVGILAAVIPTTNPTSTAIFKTLISLKTRNGIIISPHPRAKGCTIAAAKTVLE
                     AAVKAGAPEGIIEWIDIPSLEMTNIVMKECDMILATGGPGMVKAAYSSGKPAVGVGAG
                     NTPAIIDESADILLAVNSIIHSKTFDNGMICASEQSVIVMEKVYDKVKKEFKDRNCYF
                     LNKEETEKVRKTIIINGGLNAKIVGQSAFKIAALANVVVPESTKILIGEVTSVELSEE
                     FAHEKLSPVLAMYKAKDFEDALSKAEHLVADGGYGHTSSVYLDTLRAKVKLDQFIDRM
                     KTCRVLINTPSSHGGIGDLYNFKLTPSLTLGCGSWGGNSVSENVGVKHLVNIKMVADR
                     RENMLWFRTPEKVYIKKGCLPVALDELKNVMGKKKAFIVTDNFLYSNGYTRPVTDKLD
                     EMGIQHTVFFDVAPDPSLACAKEGAKAMRAFNPDCIIALGGGSAMDAAKIMWVMYEHP
                     EADFMDMAMRFVDIRKRVYTFPKMGEKAYFIAIPTTAGTGSEVTPFAVITDETTNVKY
                     PLADYELMPNMAIVDADLMMNAPKGLTAASGIDAVTHALEAYASMLATDYTDALALKS
                     LKVVFEYLPKAYDDGPNDPKAREKMADGATMAGMAFANAFLGVCHSMAHKLGAFHHLP
                     HGVANALMIDEVLRFNASEKPVKMGTFSQYGHPHTLQRYSEIADYLGIKGKTDKEKLD
                     NLIAALDELKAKVGIKSRIMDYNIDEKDFLNRLDEMTEQAFDDQCTGANPRYPLMSEI
                     KKMYLNAYYGKQEEV"
     gene            46748..47497
                     /locus_tag="PLQ68_01765"
     CDS             46748..47497
                     /locus_tag="PLQ68_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010250206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HXK71404.1"
                     /translation="MKLDKIIAVRTTKTVYRDNDKTIKVFNEDYSKADILNEALNQAR
                     VEETSLHIPKILEVTKIENKWAIVSEYIQGKTLAQIMEEDKSRFDEYLERFVDIQIEM
                     HSHKVPLLNKLKDKMNRKISESTLDATTRYDLHTRLESMPKGTKLCHGDFNPSNIIIT
                     KDDIPYIIDWSHATMGNASADVARTYLLFWLSGDINGAEKYLDLFCKKSDTAKQYVQK
                     WIPIVAASQSVKGKPEEREFLLHWVNVVDYQ"
     gene            47532..47774
                     /locus_tag="PLQ68_01770"
     CDS             47532..47774
                     /locus_tag="PLQ68_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002848998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HXK71405.1"
                     /translation="MKITVCIGSSCHLKGSRQVVESLQFLISENDLKDKIDLSGTFCM
                     NNCQNGVCVTVDGKMYSLTPETTKSFFETEVLTKLK"
     gene            47797..49473
                     /locus_tag="PLQ68_01775"
     CDS             47797..49473
                     /locus_tag="PLQ68_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015557767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HXK71406.1"
                     /translation="MTEFLRFKQSNCKNCYKCIRHCPVKSIRFSGNQAHVIGDECILC
                     GQCFVVCPQNAKEIADNTEIAKVLISSGQKVIASIAPSFIANYNGIGIKSLDKALRTL
                     GFYAAEETAIGAKLVKDKYDEMLKQGDKDIIITSCCHSVNLLIQKHHPKALPYLADVL
                     SPMQAHSMDIKRRYPGSKTVFIGPCLAKMDEADRYAGITDCVLTFDDLTRWLEKEDIR
                     LEEIPDEDQFSKTRLFPTTGGILKSMLCEQDNYTYIAIDGIENCKAALKDIEEGGLHK
                     CFIEMSACTGSCIGGPVMEKHHATPLKGYKEVNRYAGKEDFKVEPLESQIMKKNHLPI
                     IIKNDMPSENEIVNILAQMGKTKPEDELNCGSCGYDSCREKAIAVYQGKADYTMCLPF
                     LQARSQSFSDTIITNTPNGIFVLNDNLEVQQINKAALSLMRIRSASDVLGEPVVRILD
                     PKDFITVKTTGVSIKDKTVYLAEYQKYVDMTVIYDREYKIILCIMRDVTEEYKIKAKK
                     EIVNKQTVETADKVVDKQMRVVQEIASLLGETVAETKIALTKLKETISDE"
     gene            49466..50638
                     /locus_tag="PLQ68_01780"
     CDS             49466..50638
                     /locus_tag="PLQ68_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HXK71407.1"
                     /translation="MNNLCADIGYNSLNKYKEQLCGDHIECINQDDGSTVIVLADGLG
                     SGVKANILSTLTAKIISTMMAESCGIDECVSTIAATLPVCGVRGIAYSTFTIIRIVNN
                     NYAEIIQYDNPHVILLRDGNSVEMPKTLLSIDNKAIYYSKIALQENDIFIAMSDGAIH
                     AGVGMSLNFGWQRDDIIKFMEKLYHVGFTAKTLTTILLDECYSLYGGQPGDDTTVCTI
                     RIRKREPMNLIIGPPFNKDDCSKMMSLFFSKEGKHIVCGGTTSKIAADFLGKTLIPSL
                     NFVDPEIPPTAKIEGVDLVTEGVITINKVLTYAQDYLKDNESYRHWSYKKDGASQIAR
                     LLFEEATDINFYVGKAVNPAHQNPDLPIHFNIKMQLISELAECLKKMGKNIKVSYF"
     assembly_gap    50677..50776
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <50777..51875
                     /locus_tag="PLQ68_01785"
     CDS             <50777..51875
                     /locus_tag="PLQ68_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015558166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="monomeric [FeFe] hydrogenase"
                     /protein_id="HXK71408.1"
                     /translation="FNAITFDNRQKAHIDKTKCVECGMCAKVCPYNAILNNKRPCENA
                     CKVKAISMNEHRAAIIDNDKCTACGACVYQCPFGAITDKSFILNVIDILKKSSDNKKY
                     KVYAVVAPSISSQFSYAKLGQVISGIKKLGFHTVVEAALGADMVANEEAKELYEKEFL
                     TSSCCPAFVSYVEHSFPNLIKHISHNLSPMATIAKYIKDRENGAKVVFIGPCTAKKQE
                     FQKEHVHKYVDSVLTFEELQALFDSKDIEIMALDEDVLDNASYYGRIFARSGGLADAI
                     RQAIKENNLDESKYNPLSCDGIEECRAALLKADRNILQNNFIEGMACVGGCIGGAGCL
                     THGTKDKTEVDRYGMQAYEKKISDAITMIKT"
     gene            complement(51944..52099)
                     /locus_tag="PLQ68_01790"
     CDS             complement(51944..52099)
                     /locus_tag="PLQ68_01790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK71409.1"
                     /translation="MEKAEKWVMCEGEYFYCMDQFDQDQKYIPSSMFRTKSNSLEDSD
                     DDDSSYD"
     gene            complement(52256..>53488)
                     /locus_tag="PLQ68_01795"
     CDS             complement(52256..>53488)
                     /locus_tag="PLQ68_01795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK71410.1"
                     /translation="KDYIENLAKWITSAQRQSLAVIHLQKPESENIDKENEKEKDNED
                     NEIEKEEKKESLPDLQEIAEVFNKLAYSLRAYAFFFFIPHTKRHMLLQLISKNSNLTD
                     TDSDYDNNDLFIIDGNKYKDAKTDTYNMADFISDINVKDSVYKRVKKYYEKKDVDFNG
                     VEFVPDLRKNSLMEFLKTKRSKEELLRQLESSNNDYEYLKKSFNAEKENLIAENSQKL
                     NAKDEEIRKYKEKLDENREYIKEIETEFEECEEKIKYISKRIQFLRNRPKKFKDIPQW
                     VETYFLNRLTLCKRACDEIEKIKNEYDFDFICDAIEHLALDYYEQLCGEITEDIRNDT
                     CSKVYNSYIEITKVNPPKITNTKDYEFTYNKKDGSLVKAEIDRHLYLGSEGRIYFIFD
                     KEDKRIVIASLPKHLNID"
BASE COUNT        18136 a         8128 c        10424 g        16600 t
ORIGIN      
        1 cgtatatttt aaaattagag tgttggataa aaggtatttt gaaggaagga agtacggtaa
       61 atgagataca caggtgtaac atcaagagga attataactc caatatttaa acaaggtgat
      121 gatttagtat cattagtatc agatagtata gtaaaagctg ctgaaaatga gaattttact
      181 atttctgaca gagatgtaat aggagtaacc gaagcagtag tcgcaagaac acagggaaat
      241 tatgcaacat gtgaacagat tgcaaaggac ataaaaagta aattaggcgg taaggatatg
      301 ggtatagttt ttccaatatt gagccgtaac agattttcaa tattgttaaa agcaatcgct
      361 atgtcctgtg aaaaactgta catacagtta tcatatccat ctgatgaagt gggaaatgcc
      421 cttatagcac ttgatgagct ggatgaatta ggggttaatc cgtataccga cagttttact
      481 gaatcggaat tcagaaaact atttaaaaat actgttcatg tatttacaaa agttgactat
      541 atagaagcat acaagagcat gggaaaagac attgaaattg tattttccaa tgatcctaca
      601 catatattga aatatacgaa aaatgtttta aattgtgata tacattcaag gaagcgcaca
      661 caaagactta ttaaatcagc tggagcagaa aaatcataca gacttgatga gatactgact
      721 tcaagcatag acggttcggg attcaataaa gattacggtt tattagggtc aaacaaggca
      781 accgaagaca aagtgaaact ttttcctcgt gatgctgaaa cttttgtgga aacactgcaa
      841 aaaagaatgc ttgaattaac cggaaagcat ttagaagttc ttgtgtatgg agatggatgt
      901 ttcaaagatc cggttggagg catatgggag ttagcagatc ctgttgtttc gccaggtttt
      961 acaaacggac ttcatggtac gcccaatgaa ttgaaaatga aatattttgc agataacgaa
     1021 tttgcctcat taagagggga acagcttgct ttggctatga aagagagaat taaagcaaaa
     1081 gacaaggatt tatctggaaa catggaatct cagggaacta ctccaagacg acttactgac
     1141 ttattaggct cactgtgtga tcttacaagc ggtagcggag acagaggaac acctgtagtt
     1201 cttattcagg gttattttac caactttgca agcgactgat gacatataaa tttaaagaga
     1261 ttacagatac agtaaagaag attttcataa acaacaaagg aagtcatgac tgggaccata
     1321 ctgaaagggt cttaaatatg gcagagaaaa tcgctttatc agaaaacgct gacatagata
     1381 tagtaagata tgcagctgtt cttcatgaca tatcaagaca tgacgaagat atgtccaaag
     1441 gaattattca tcatgaggaa aagggagcta tagaagcaag aaaagttctt tcatcatttg
     1501 gtattgatga tataacagct gataaggtat gtcattgcat tctgacacat aggttcagaa
     1561 gaggcaataa gcctgaaact ttagaagcaa aggttctttt cgatgcagat aaattggatg
     1621 gaatcggagc tattggtatt ggacgagctt ttgtgtttgc aggggagaac aaagccagag
     1681 tacatgaccc tgatgtggat gttgagaaaa caaaaagtta tactaaagaa gataccgcat
     1741 atagagaata tctggttaaa ttatcaaaga taaaagacag aatgctcaca aaatcaggaa
     1801 aagaaatagc actgaatcgt catcagttta tggtagagtt tttcgacagg ttgaatgctg
     1861 aatgcaaagg tattgtctga gtatattaat gattgaataa ttaacattgt aatgatagaa
     1921 tatttgtata ttaagtgttt aataaagtgg gggtagaaaa tgaataggga taattttatc
     1981 aaacagacaa cagaacaatt gaccaagatg aatgttccat ataaggttca ggatggaaca
     2041 gacataagta tttctgctac attcaagaat aaagatgatg attctgaaga tattctctac
     2101 actgcttttt tctttctgaa tccagcagat aataaagtgt atgcatatca aaaagcttta
     2161 gcaggacaga atgatgtttc taatgatcct tcttttgtta atatgaatgt taaaagaaat
     2221 gatgatgatt cattggtgcc tattgggctt atattaagtt cgattgcagg tattgcagct
     2281 tctttaggat ttgtatttca aagaactgat aaccagcaaa tggctgtcta tccgaaagga
     2341 tatattccag catcggttgg agcagctgca caaggtacag ctttttgtac cagttgtggg
     2401 gcaaagatgc ctataggaac aaggttttgc accaactgcg gagctgattc acaagctcct
     2461 gcagtaacac agccaaaacc atttgtttca aatgatgctc cacctgttta tgcgaatgtt
     2521 aatcaaaacc ctttaccatc atatactcag ccaaatatgc agaatatgaa accaagaaga
     2581 aagacccctg ttgttatagt tgtaatagca gtagtgttga ttattggagc tttagtgact
     2641 ggtggaatac tgcttagaaa ctggattgta aacagaaaac cttcagacaa tgtaaatgtt
     2701 ccaaatgctt ctgaaaatgc gttatctggc acaaatccta ctcctacagg ttcatcttct
     2761 tctgaaacag gttcttcagt agtttcagca ggagtaattc actccgattt aggtaacatc
     2821 atgaacggcc agtattactt tgctaccgat gagtatattt tctattcaag ttatgatgct
     2881 gattttaaag ctcatatata ttctgtcaaa aaggacggaa ctgaacataa agttatattc
     2941 aacgggtttg gatggtcttt ggttgttatt gatgactggc tttacttttc cggtaatcag
     3001 ggtgatgtca ttgatgggac atacaatatc tttagaatga gacttgatgg aagttctgtt
     3061 gaaaggatta ataatacata tagctatggt atgtttttgt atgatgaata tctttactat
     3121 atgaagatga atactgatta tacggatacg ttttccatat gcagatcatc tcttgacggt
     3181 tcaaatgaag agattctttt cccatatggg agtaaccctg tcgtttatga tgatcttctc
     3241 tattactttg ataatcaggg aaatatgttt agaacaaaac ctgatggatt ggaccctgaa
     3301 gtactaataa caggtgaagt tggattctat gttttaagta atgacaagct tatttataaa
     3361 gatttcagtt caaatattta tacctgtgat cttgatggtg gtaataagaa acttgtaaga
     3421 tcttcatcag ggaaaagtat tttgactgtt aactcatata atgaaaggat atattttgtc
     3481 gaatataatc ctgagttcaa ttattcaaca tattcatggg actatgacgc tattagcatg
     3541 aaaatggatg gatctgatga aaaagtaata ttttcaagtt catcatatgg tatgtacatg
     3601 aatatagtag acggcaaact tatgatgatg gattatttaa tggatcctca gactgccatt
     3661 tattatactt acattaatat aatgaatctt gatggcacta atttaagagt gcttgaaaga
     3721 tagtattttt ttctataaaa aatatgatat aatattgctt ggaaggagaa taaaagatgc
     3781 caggtatttt tagttttgtt gagaacatta acgttctgca agcagtatta cttgttattg
     3841 gtttgatatt tttaataatc gaagcatttg taccgagttt tggtttattc ggagtgcttg
     3901 ggatagtgct tcttctactg tctatcatcc tgactgccac gacatttgtc gaagggttgg
     3961 ttatgttcct tattattctt attatcgcag ttgttctgtt gattatagct ataagattag
     4021 ctaccaaagg taagttatcc aaaaagctta ttaacagaga tactttttct gataaagaag
     4081 gatatttcgg aattcaggac tggagcgctt cagtaggatt gaaaggaaaa tcaataacca
     4141 ttctaagacc agcaggaaaa ggtgagtttg aaggtaacac tcttgatgtt gttactaaag
     4201 gggagtttat agagaaagat aaagacatca tcatcgaaaa agttgaaggt gcaagaattg
     4261 ttgtaaaaga aaataaagga gagtaaataa tggatccaat tgtattagtt ctagttatca
     4321 tagctgcagt aatagcattt tttgctatac ttctcagcct tgttccaata gggttgtgga
     4381 tatcagcaat agctgcagga gtaaaagtag gcttaatgag ccttatcgga atgaagttta
     4441 ggagagttga acctgcaaaa atagtcaatc ctcttataaa atcaacaaag gccggtttaa
     4501 acgttacgat aaaccagtta gaaacacatt atcttgcagg aggtaatgtt aacaaagtag
     4561 cagacgctct gattgcagct caaaaggcgc gtatcgacct tacatttgag acagcagcag
     4621 caatcgacct tgctggaaga gatgttttgg aagctgtaaa aatgagtgtt aacccgaagg
     4681 ttattgaaac acctataatc gctgcagttg caaaagacgg cattgaagta aaagccaaaa
     4741 gcaagattac agttcgtgct aatatagcaa gacttatagg tggtgcaggt gaatcaacaa
     4801 ttatagcaag agtaggtgaa ggcattgtta catcagtagg ttctgcagaa tcatataaag
     4861 aggttttgga aaatcctgat cttatatcca aaacagtact taaaaaaggg ttagacgcag
     4921 gaactgcttt tgagatactt tcaattgata tagctgatgt ggatgtcggt agaaacatag
     4981 gtgctcagtt gcagaccgat caggcagaag cagataagaa gattgctcag gctaaggcag
     5041 aagaaagaag agttatggct gttgccagag agcaggaaat gaaagctaat gttcaggaga
     5101 tgagagcaaa ggtagtagaa gcagaagcta tggtaccaag agcaatggca aaagctttag
     5161 aagaagggaa tcttggagta ttagattact ataacatgaa gaatgtaata gcagataccc
     5221 aaatgcgcga atccatatct gaaactggca aatcgtcaaa aactcaaaac gacaagaaag
     5281 agattaagta atgagcaggt atttaaaaaa agatgatgat atgtctaaag tcatagaaga
     5341 attcaaacct tcaattgttg agaaaaaact tgaagagaag gatgattatg acttaataag
     5401 tgatttgcta gatgatagtg aagtattagt caaaggcatt attttaagtg agatacttgg
     5461 taagccaaaa gctaaaagat agttgataaa aaacaaatta aaatccgctt aaatgcggat
     5521 tttttttgta gttttttatt tagactaaac tatgtttatt cttattctac agtcactgat
     5581 tttgcaagat tttttggttt atcaatatca cagtcatttt ctaaagcgac atagtaagcg
     5641 aataactgta aaggaataac ctgtgcaact gcattgaata gagtatttcc ttcaggaata
     5701 tatattatgt catcagattc tggtattatt ttcgtactgc gttcagtgat aattcctaaa
     5761 acttttgcac cacgagcttt gacttctctg atattactca ctaatttgtc taataggcgt
     5821 tcattgcatg ccaatgcaac tacgagtctg tcattatcaa taagagctat tgttccatgt
     5881 tttagctcgc ctgcagcata tgcttctgaa tgaatatatg atatttcctt aagtttcaag
     5941 gaaccttcaa gacaaactgc ataatccgta tttctgccaa taaaaaacat ggatctttca
     6001 ttgtggtatt ttctagccag atatggtatt tgaggattaa ggtcaattgc tctttgggcc
     6061 aatttgggaa tctgttgaat atcttcagtg agagaagatg cagtcttttt atcgatactg
     6121 cctacttttt gagcaagaaa tactgataaa agacataaga cagtaacctg tgtagtataa
     6181 ccttttgttg tagctacagc tatttccggt ccagcccatg tataaataac ataatcagca
     6241 agttttgata ttgctgaacc tacaacattt actattgcca atacaggtgc tcccaattgc
     6301 tttgcctctt tcagtgctgc tatggtatct gctgtttcac ctgattgtga tatgactatt
     6361 acaagtgttg atttatttat aataggattt ctgtatcgaa attctgatgc aaggtcaact
     6421 tctactggta tccttgctag ttgctcaatc atatacttgc cggtgcaagc agcatggtat
     6481 gctgatccgc atgcagtaat tacaattcta tttatgtctt tcatgatgtc atcaggcatg
     6541 cctgagtcat caagtgttat ttttcctttt ttaattcttg gttctatagc agattttatg
     6601 gctctgggct gttccattat ctctttaagc atgaaatgct catatccgcc tttttccgca
     6661 gcttctacgt cccagactat tcttgttatt tctcttgcta caggctctcc tgtgcagtca
     6721 aatacattta ttttttcgct tgtaatttca gcaaattctc cctcctcgat atatataaca
     6781 tttttagtgt gttgaaccag tgctgtaaca tccgaagcga aatagttttc tgacaccccg
     6841 actccgataa tcaaggggct gttgagcatt gctgcataaa tcgtattagg ataatctttg
     6901 cagataacac ctaatgcata tgagccttcc agtcttgaaa ccgttttcat tactgatttt
     6961 ttaaaatctt tgtcatagta catatcaaga agatgaacta ctacttctgt atctgtctcg
     7021 ctgttaaact tatatccttt atctacaagt tctttcttga gatccatata attttcaatt
     7081 atcccattat gcacaactgc gaatttcttg ttattgctga catgaggatg agcattaata
     7141 tcatttggag caccgtgagt agcccatctg gtgtgagcta ttccgatatt gcccgaaagg
     7201 ctacaaccat cattagtctt atcacaaagg ttcttaattt taccagttgc ttttaccata
     7261 ttaatttcat ttttatcaat aacagctatt cctgctgaat catatcctct gtattccagt
     7321 ttttttaagc attctataag aattggagct gcattttcat ttccgacata acctactata
     7381 ccgcacataa aacctcctat ttgaaattaa tttacatatg cgattttact gcgttaatct
     7441 ctgtatgtca aataagtata gttacttttt tgtttgggaa agcattttat gcttatattt
     7501 aaacaaatag gagatatttg caatatgtta aatgaacaaa aatggtaaat aatggtataa
     7561 ttagaacaaa aggagtaaca atgaaaaaga ttagattgaa gattgatatt gcagattctg
     7621 aatattcaga tagattatca gaatatataa aaattaaagg tttgaacata tttgaaatta
     7681 ctgattttaa ccagcatata acattatcag atttacgtgg tgaagactca gactgtcttc
     7741 atttattatc tgaaaaagag gatgtgttaa tcagcaaata ccagtcagct gacagaataa
     7801 ttcaatctgt aatggaaaat gtcagtaaat gcaatgaaga gactgtaaaa attgtaagca
     7861 gaaattgtag agttattttt gttacatcag gtcatggcgg tgcagggaaa agctgtatat
     7921 cacaggcatg tgctatttat tgggcacgaa aaggtaaaaa ggtcatgtat ataaatgttg
     7981 atgggttcag tgtgaacgag agtatattta ataccgaaaa accatatgat atatcccttc
     8041 taatgcatta tacaaataaa gtaaatagtg attttaatgt ttatattgaa agatacaaga
     8101 atcacgactc agaacacgat gtctgttttc tttcaagtgt atacccctca tgtgatactt
     8161 gttttaatta tgaacaagga cagtatttta tgaattcact tatatcaaat gatatttatg
     8221 attacatctt tatagattgt cctttatatg caaataaaac atatatagat atgatgaaac
     8281 ttgcgaacaa aaccattttt ataaagaata aaggcagtaa aagagaagaa gaagttatac
     8341 gattcctcga aaacaatgga atcagtcttt taatagcatg taatatggtc agatatacag
     8401 aaggtgttta tataccnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnatat
     8521 tggactcaat aataggctac ggtttcatac agcctcttat gactagaaaa ggcattactg
     8581 aaattatgat taattccgat aaaatctttt ttgaagaaaa aggaagattg cataggtaca
     8641 ataagaattt tactagtcag gatgttgagc gacttataaa tatattatgc agaaaaactg
     8701 accggacggt aaatatgagc tgtcctatac tggatggaag tattggtcat gatattaggg
     8761 taaatatagt tctttctcct atagctaata atggtgacag tataacaata agaagatttc
     8821 cgcagactcc tattactgat gaagatatgg tgaatgaggg gttttttgat aaagaaatta
     8881 gagatttttt aagttacgca gttaaaaaca gatataacat atttatctgt ggcggagcag
     8941 gttgcggaaa gacaactcta ctcaatgtca tgagtacatt tataccgaag gatgaacgag
     9001 ttattactat tgaagattcc gtggaactta tgatgaataa gatagataat gtaatacgct
     9061 taatttgcag aaatcctaac agcgagggaa aaggaaaaat tgatatcggt gaccttataa
     9121 aaagcagcct tcgaatgaga ccggacagga taatagttgg cgaggtaaga ggaggagaga
     9181 ctttatatat gcttcaggct atgaatacag gtcatatcgg gtctatatcc acaggtcatg
     9241 caaattcatg taaagatgct ttttcaaggc tagagaccat ggtacttatg gagtgcgata
     9301 tgccacttct gtcaatcaga aaacagatat gcaacgcttt agatataata atatgtctag
     9361 gaaggataaa tggtaaaagg actgttttgg agatatcaga agtattgggc tttgaaggaa
     9421 atgaagttaa gcttaatatg ttatacatat atgattttaa taagaaatat catgaaaaag
     9481 taaatagcgt tattcgaaca gagaaaatgg atagaggtat gtacaatgag atatgaattt
     9541 atcaaacatg cgataacggt tggttttgca tatggatttg ctgcgtattg tctttatcta
     9601 tcacagcttg ttgcaatatc cgtgttttta atagtaatgg taatctgtct tttgaataaa
     9661 aaacaaatgg atatgctaat atcaaaaagt atgagagaca gttttgaaag atttcttgaa
     9721 atactggcat cttatgtagc catacctggt aatttcatgc atgcatttac aaaagcttta
     9781 gcagaacatg aaaagatata tcccaaagac attcttaatt gtgcagctaa gaaagcatat
     9841 atcaagtgct ctgcaaatat tccttctgat ataatcctaa cttcatttgc gaaagatatt
     9901 aatattcagg aagggtacct gttttgtgaa agtcttgtaa tatgtgaaaa aaaaggtggg
     9961 gatatcaata ggactatacg agagactatt gcatttatta aagaaaaaac cggtatagaa
    10021 catgaaattg aaaccataac cgcggaaaaa agaattgaga gaataattat tagcatatgc
    10081 ccgtttggaa ttttagcatt acttaattca atgggtacag gctaccttga tgttttatat
    10141 gaagggcttc ttggaagaac tatcatgaca attgcaggag tactgttcct gatttcatca
    10201 tatatagcta agaagatact ggaggtaaga atatgatact agtttttgtt tatattgcgc
    10261 ttatactgtg tatatatatt ttttccaaaa aaagtttatt cgaattttct ttaaaacact
    10321 ccaaatatct caagcacttc tataaagctt ttattccatt atctaaaaat ttcttaaaat
    10381 atgcatcagt ggataataaa atgtttttaa tctatcctga aaaatcagta atagatataa
    10441 ctattgttca tatcagtatg aaaatatgta tgttctttat tatgcttttt cttcctatgg
    10501 tcgcatttgc agtaaatgct tctacatatt taaaagtgat ttcatttgtt atgtgcatta
    10561 tgtcggtaaa atgcattgat ttggatatta agagtgaata tgaactgtta aaggaaaaga
    10621 ttttaagtga cttttctata tatcttacaa acataacatt atatattggg tcaggtatga
    10681 ccttgtatca ggcatttgtt cagcaggata taaatatgcc agaaggcata ttaaaagatc
    10741 agatgaagaa acttataaaa agcattgaat tgagatcaag tccggaggat gcctttttga
    10801 gtttatcgat tgcgcttcca ataacacaaa tcaattcatt ttgctcaatt atgatttcag
    10861 ggtttaagaa tactgaatca ggtttgaaag atactctgaa agcatataca tcatcaatat
    10921 ggacagaaaa aaagaacaga gcaaagataa gagctgaaca ggcgggtgca aaggctgtct
    10981 ttgcattagc aatcggactt gtagggattt tactgatact tattgcacca gcaatgctga
    11041 tgcttaatag ttaaagaaag gagtatgtta tgtataacag attaaacaaa aaaggtatgg
    11101 gtactgtaga ggtagtaata atactggcag ttttagtggg tattgcactc atattcagag
    11161 aagctattac tgatttcata caaaagataa tagacgggac actgaatgat gaaaccataa
    11221 tagaaaggct taggattaat taagtatgat tagcgaaata aaatcaggta atagtcaggt
    11281 gcttataagg gttatgggac aaaacatgga atgtgctcca tatatgatta aaatgttgga
    11341 gaaaacagag tgtaatttat tgctgcccat aaaatatgca actgatgagg agcaactgta
    11401 tgatactgtg tatggactaa gtttagatgt atatatgaaa aaatatggtt atactcctga
    11461 ccttcttaag aaccttatgt actctattca gagcttatta agggtatcag agtcttatct
    11521 tattgataca gaccacatcg tattggatac tcagtatata tttgtatatg ataatagtaa
    11581 ttttaagtat atttttgatc catataacaa acagaataca aatgagaata taaaaaaact
    11641 ggtaaattcg attgtttcag gaaattttgc gaataaggcc ttaacagaag cgatgaatca
    11701 acagtctttt agtcttaaac tcgttattga ttcattatct gatgaaaaag atgaaaaaat
    11761 gattggcggt ttagaagaag atgaagcagt aatcctacgt aacattattg atgcagatga
    11821 ctgtataaaa gtttcaaaga aggatgtgta tgttggaaaa ctacccttaa ttaatgataa
    11881 aagacttatt gggaaacttt catcttttcg acatggatgt ataagatatt cgagaaagtc
    11941 tgtgtacatt aaggatttaa acagtaaata tggtatatat gttaatggag aaagaataaa
    12001 atccaatact ttttttagta tccataaggg ggatatagtg gcatttggag acgatgagta
    12061 tatattattg taggttaaaa aaatagtatg gatatctact gttatttatg ataaaattct
    12121 ttgtaatgaa tataaggaga aaacctatga tagcaatagg ctgtgatcac gcaggtttta
    12181 agttaaaaag tgagattctg tcgtatctcg ataagaaaaa aatatcgtat agggactttg
    12241 gagcttatag cgacgattcc tgtcattttc cgttttttgc ctctttagta gcaacagccg
    12301 ttcagaagaa cgaatgcgaa agaggtattt taatatgtgg aacaggaatt ggcatgagta
    12361 tagcagctaa taaattcaaa ggaataagag ctgctctttg catggatgaa taccaggcac
    12421 gcatggcaaa aaggcataat aacgccaata tacttgtgtt agctggacgt ataacgccta
    12481 aggaaaaagc tcaggatatt attgacatct ggcttaatga aaattacctt gaaggtaaat
    12541 atgaaacaag aaaccagttg attcgcagaa tagaagaagg aggaagtatg tcaggaacta
    12601 attacaaaga tatggcattt gtgtttgacc acccgctcat acagcataaa atatctatta
    12661 tgagggataa gaatacgaat acaaaagaat ttagggagct tgtcaaggaa gtatctacat
    12721 tgatgggtta tgaggtcaca cgtgaacttc cattggaaga tatagaagtg gaaaccccaa
    12781 tagcgaaaac tactgttaaa gtactttcgg gaaaaaatgt cggttttgtc ccaattctta
    12841 gagccggact tggtatggtt gatggtcttt taacacttat acctactgca aaaataggac
    12901 atatcggtct ttacagagat catgatacat tagaacctgt tgaatattat tgtaaactac
    12961 ctgaagatgc acatgaaaga atgataattt tacttgatcc tatgcttgca acaggcggat
    13021 cagcatctgc agcaattggt ttcttgaagc aaagaggcgt aaagaatatt aagcttatga
    13081 gtataattgc agctcctgaa ggtattgaaa aagtacacaa ggaccatcct gatgttaata
    13141 tatattgtgc atgtgttgat aaagaactca atactaatgc atatattgtc ccgggattag
    13201 gagatgccgg agacagattg tttggtacaa agtaatggac agaccctcgt gggatgagta
    13261 tttcatgcag gttgtccatc tggttaagac gcgttcaaca tgcttaaggc gtaaggttgg
    13321 agctgtaata gtcaaagaca aaagaatttt ggctaccggt tataacggag ctccaattgg
    13381 atgcagacac tgtgaagaag ttggctgttt aagacaaaag ctgaatgtac cctctggaga
    13441 aagacacgaa ctatgcagag cgattcatgc agagcagaat gctattgttc aggcagctag
    13501 agcaggtaca tcaatagatg gagcaacaat atatgtttca gctcaaccct gcgtaatctg
    13561 tgctaagatg cttattaact caggtattaa aagaattgtt tttgaagggg attatcctga
    13621 cgaattatct aaggaactgc ttgatgagtc aggtttagaa ttggttaaat acggaggatg
    13681 atttgagtta tctgtatacc tttttagcat catttattat tgcctttgca ttaagtcctc
    13741 tggtaatcag atttgctaaa gcaaaaaaaa tattgaagta tcctaaaaga aacagggatg
    13801 tgcatactga accggtacct ttacttggag gtaccgctat aattatctct ttttcgattt
    13861 ccattcttct gaattattta ataaacccta atttttatgt taccatcgag gtaatagggc
    13921 ttgcagtggg tatagtaata atatcattta tgggtattct ggatgatata tatgatctta
    13981 agccagttat aaaagctggt tttcagtttc ttgctgctat tgcaactgtt ttaatatcag
    14041 gttcaaaaat tgcatatttt acgaatcttg ggcaaaataa caatcttgtt tatttacccg
    14101 aaatcatttc tttcatagct acagttttgt ggatattcat gttaacaaat gcatttaata
    14161 taatcgatgg tttggacggg ctgacgacag gaacaactat tatttgctct ttgacattat
    14221 tctttattac tttcgttcgt cctgatgcaa atctcgtcgg agaatatata cctatacttt
    14281 taatagcgct tagcggagct gctgcaggat ttcttccttt taactttaat cctgcaaaac
    14341 tgtatcttgg agagacaggt gctgcatttt taggttttac tgttgcggtc atttcaattc
    14401 agggaacaat gaaagctcat gctgcaattg ctgtatttat accattaata gcaatggggt
    14461 tgccgatact ggatatatca ttggcatatt ttagaagaat aattatgaaa aagcctattg
    14521 cagtaggcga ccgtgaccat attcatcaca ggcttatgaa aatgggatta tctcaaagaa
    14581 aaactgtttt gatactatat tttataaata ttgtatttgg agtcacagca tatttgttat
    14641 caggtcagca atacagcaga ttctggatat actttgcgat agttttaatt ttactaggat
    14701 tagcagtcta tgtactatat ggaccattac tcaaacaggc tgcaaaaaat ggaaatggga
    14761 attcagttac acaagaaaag gaggaatcag aggatgatta aggtcatatc agtttttgga
    14821 acaaggcctg atactataaa gatggctcct ctggtggctt tgcttgataa agatgaatct
    14881 tttgaacata tagtatgtgt tacaggacag catagggaaa tggtcaaaca gattcttgaa
    14941 gtgtttgata taaatccaaa atatgattta gatataatga agcaggggca gagtctggat
    15001 tatgtcactt ctgcaatact tgtacgattt agtgaagtac tggatataga gaaaccagat
    15061 atgatattag tgcatggcga taccaataca tgtttatctg cttctattgc agctttttat
    15121 aagaaaatac ctattggtca tgtagaagcg ggtttaagaa ctgaagacat cttttctcca
    15181 tatcccgagg agttttgcag aaggacagtt gattcaatag ccaccttgta tttcgctcct
    15241 acaaaaagta atgcaaataa tttaattaat gagaaaattg atacagacag gatattcatt
    15301 acaggaaata ctgtaataga tactcttaaa acaacagtaa aggaaaacta tatctttaat
    15361 gagccgatgt taaatgatat agactataaa aacaaaaaag ttattcctat aacagctcac
    15421 cgaagagaaa atataggtat aaatctggaa aacatatgta atgcagtcaa aacacttgct
    15481 aaagaataca aagacagcca ttttatttat acagttcatc caaacccgaa ggtaagaaca
    15541 acggcagaaa agattttgtc tggtattgga aatgttacat tactgcctcc attagttttt
    15601 actgaccttc ataatttaat ggcaagagca tactttgtaa tgacagattc aggcggtctc
    15661 caggaggaag ctccttcatt aggagttcca gtccttgtat taagaaaaga gactgaacgg
    15721 ccagaagctg ttgaggcggg tactgtcaaa ctgacaggag tgaaactgaa cgatatatta
    15781 actgatgcaa aaatgttatt tgatgaccaa acattttatg caaagatgaa aaatgcagtt
    15841 aatccatatg gtgacgggaa cgcatctcaa agaatagttg attccataaa agcatatttt
    15901 aataatcttt agttatttta ttagtttatc tatcttgtat tttgattttt ttgatatatg
    15961 aggtataatc caaacggtta ttttagtgaa agatttttta tgaatgaaag aaatataata
    16021 tataataaat tacagaggta aaaatgcagg acaaattaag gaacatagaa agaagatatg
    16081 aagagataaa tgcacaacta tctgatccgg atatattggc caatcaggaa aagtatgtag
    16141 ctttaatgaa agagttgaat gaaatttctg agatagttaa agtgttcaga gagtatacag
    16201 aaacattgac aggtattgag gatgtttatt taatgctgaa ggaaaattct gatgatccgg
    16261 atttcaaaac aatgcttgaa gaagagctgg aaatgtttga agaaaaaaaa cctgtattgg
    16321 aacaaaaact taaagtactt cttctgccga cagatcccaa cgacaacaaa aacgttatca
    16381 ttgagattag aggtggagcc ggaggagatg aagcagcatt gtttgcagca aatcttctta
    16441 gaatgtatac aatgtatgct gaatcaaaaa agtggaagac agaggttctt gatttaaatg
    16501 agataggtat aggtgggatt aaggaagttg tattctcaat aaatggtaat ggagcataca
    16561 gcaggtttaa atatgaaagc ggtgtacaca gggtccaaag agttcctgaa actgagtctc
    16621 aaggaaggat acatacatca acagtaactg tagctgtctt gcctgaagct gaggaagtgg
    16681 atgttgattt aaatcttaat gatgttcagg ttgatactta ccgttcatca ggagctggcg
    16741 gacagcatgt aaataaaact gattctgcaa taaggcttac tcatctgcct actggtatag
    16801 ttgttgcctg tcaggacgaa agaagccagc acaagaataa agataaagca tttaggattt
    16861 taaggagtaa actatatgat cttgctcagg aaaatcagga taaagaaatt gctcaaaata
    16921 gaaaaagcca ggtaggtaca ggcgacagaa gtgagaggat tagaacatat aatttcccgc
    16981 aaggccgtgt aacagaccac aggataggac tgacgttata taaaattgat tctattatga
    17041 atggtgatct tgatgaatta atggatgctt taataacaga agatcaggct agaaagctgt
    17101 caaatcagga tgaataatat aaagacaagg ctttttaaca ctgtaaatga agagagcctg
    17161 aaagaaagcg cagatataat aaaaggagga ggcattgtag cgtttcctac tgaaactgtt
    17221 tacgggttag gagcggatgc tttgaatcca aatgctattg aaaagatatt tttagcaaaa
    17281 ggaagaccca atgacaatcc attaataata catattcata atatatctgt tttagataag
    17341 tatgttaagg aaataccttc tgtgttaaca atgctttcaa aactttatat gccaggacct
    17401 cttactgttg ttttaaaaaa gaaagatatt attccaaaca atgttacagc agggctggat
    17461 acagtcgctg tcagaattcc ggataatgaa atcgcattaa aacttattga atattctaat
    17521 accccaatag ctgctccaag cgcgaatata tcaggcagac caagtcctac tatgtatgag
    17581 catgtagtaa gtgatttatt tggaaaggca gacgcaatta taaacggagg aaagaccagg
    17641 ataggactgg agtcaacggt acttgatctg acaacagaaa tacctgttat cttaagaccg
    17701 ggcggagtgt catatgagag tttgaaactt atacttactg atgttgaaac agcttattct
    17761 tttgatacag aaaaacctaa atctccggga atgaagtata agcattatgc tcctattgct
    17821 gatatggtta ttgtaaaagg taaaactgat gatgtagtta gaaagataaa tgaaatatca
    17881 gatgatgatt gtgcagtttt atgctgtaaa gaaacatatg aaagatatat ttcaaataat
    17941 aaattaagtg cgggaagtat taatgatcct tcagaaattg catcaaacat atttcagtgc
    18001 ttaagactgt ttgatcagat gaagattaaa aagatttttt gtgaatattt tgaaatgaaa
    18061 gatataggtg aagcggtgat gaacagatta ctaaaggctg cttcacagaa tataatcaag
    18121 gtgtagtatg tcacagattt tagaagcatg catggtcata tgttttggta tatcatggcc
    18181 aatgaacatt attaaatcat taaggtcaag aacagctaaa ggcaaaagtc ttcttttttt
    18241 gatactggtt cttcttggtt attgttgtgc tataacaggt aagattattt ctgattctgt
    18301 taactgggtt ttttggttct atgtagtcaa ccttgtaatg gttataacag atatagttct
    18361 ttactacata aacagaaagg cagacataga agcggatgga tatactcaca ataggaaata
    18421 gtttttcgca ggatattacg acatatcttc agagaatatc taacaaagat atatatgttc
    18481 gtaatatata cataccaggt tgcagtctgg aacagcatgc gaataatatt ctaaatgaca
    18541 ataaaacata tgaatatcaa aaagatggaa gaaggcttta tatgacaaac ctaaaagaag
    18601 ctctttataa aagacaatgg gatataataa ctttacagca ggtaagtcac ttaagcggga
    18661 ttgaatctag ctatcatcct tttcttgttg cattaataca atacattgaa gaaaaatgtc
    18721 cgaatgcaaa actgatgttt attaagacat ggccttatga aaagggctgt agtcattctg
    18781 gtttccaaaa ttataacaat gatacatata ctatgtataa gtctataact aatgttgttg
    18841 aaaatacatc aaaaagatat aatcttgaaa tcattgatgt tggaaatact atatataacg
    18901 catatataaa aacatctaat ggctctgata ttgaaaaaat agaatattat cgtgacggat
    18961 ttcatctttc cataccgcaa ggaagatatt tagctgcgct agcttttcat aatcatttat
    19021 atggaaaaga tggaattaat tataaataca agcctaaaaa aatgaccaaa attcagcagg
    19081 agatactgct taatcacata taagggcaat ttgttttatc atatgttcat tgctaacaga
    19141 tactaaataa ggtataattc tattaccata ctagacgggg agttagcggt gccttgtaat
    19201 ctgcaatccg ctatagcaga gttgaaatcc cgtctgaggg ttaatagcag tggagcagcc
    19261 gcatgtaagc ggttatgagg gctgggtctt gtgcaatcga agcttgtgaa cctcgtcagg
    19321 tcttagcgga agcagcgata aatgagatac tcggtgtgcc ataagagcgc ctggcctgag
    19381 ccttttatat gtaaagcgtc tgttgttatt attcgaagac gggtgtatgg tattttttag
    19441 gagaacttat ggcgcatatt gcattataca gagaatggcg tcctacctca tttgaaaaag
    19501 ttgtggagca ggaacatata acgaagacct taaaaagggc tgtggtaaca ggtcgtattt
    19561 ctcatgcata cctttttagc gggaccagag gtactggaaa gacttcatta gcaaagattt
    19621 ttgccagagc tgtaaattgt cttgacccaa aagatggaga accatgcaat aagtgtgaaa
    19681 tatgtaaaaa agctcttgat gagactttga tggacatagc tgaaatagat gctgcatcaa
    19741 ataattctgt agacaatata agaaggattt gtgatgaagt gaactatatg ccttcgttag
    19801 cgaaatttaa agtatatata attgatgaag tgcatatgtt aagtacatcg gcttttaatg
    19861 cattactcaa aacattggaa gaaccgcctt catatgcaat tttcatatta tgcacaactg
    19921 aaccgaataa attacctgca actgttcttt caagatgtca aagatttgat tttcacagaa
    19981 taacagttaa aggtattgaa caaagattga aactgattgc aaaatcagaa aacgaaaata
    20041 ttactgatga tgcaacagaa atgatagcaa gaatatcttt tggagcaatg agagatgcaa
    20101 tcagtttgct agaccagtgt atttcttcaa ccgataagac aattgaagtt gatgatgtac
    20161 taaactgcgc tggtttaatt gatgaaaagt ctaatgccaa agtgtccaaa gcattagcgg
    20221 aaaatgatgt aaaaggaata ttatcaagcg tcaatgatgt tatagcggaa gggaaaagcc
    20281 cttcaagatt cttgtctgga ttattaggat atctaagaaa cattttagta tgtatgaata
    20341 ttaatgatcc gtcagatttt attgaagcag acagtaatgc gatagaacag atgaaaacat
    20401 tatcaaaaat gtttgatgca gatacagtta taagatatat aaaagaattg tctgaaaatg
    20461 aagcagtctt aagaagagca tcccagccaa aaacatattt agaaataatg ctaattggac
    20521 tgttagttaa agaagatgtt aaaccaatag aagaaaaatc ggagacaaag aaacaagcaa
    20581 taaaggaaat taaagaagaa aaatcatatg aaacagaaaa aaaagcatct gacatcagta
    20641 ttgaatcttc agtaaaacag ccagagaaac aatcacagag taaagcctgg tcaaaattta
    20701 tacaagacag gatttcagaa ggttcaaaag atgtttttga gttaaaagat tcatctattg
    20761 agatagtagg caatcaggct aaaattactg tacagagtac atttttaaaa gactcactag
    20821 agtataacaa agacgaggtt cttaaagaga ttcaagggta taactctgag ataaaaaatg
    20881 taaaattcgt atgttctgca aataatgtac aagtaaatac tatagaaggt gatttgctta
    20941 aaataggaaa agaacataac atagaagtag agatagaata aaggaggtag cttagatatg
    21001 gcacgtggaa tgatgggcgg aaatatgaac tataacagca tgattaagca ggcacagaag
    21061 atgcaggctg acatggcgaa agctcaggag gaattggaag aaagtaaatt tgagtcaaca
    21121 gcaggaggag gagcagtaac tgttactcta aaaggcgatt acactatgga atcgatagct
    21181 atagatcccg aaatattgga agatgccgat gttgaaatgc ttcaagatct aatactgaca
    21241 gcatttaatg aatctgttga aaaaataaat gcaaaaaaag aagaaacaat gggaaaagcg
    21301 accggtggct taaggatgcc tttttaaatg gcagacatgt atccaaaacc attatcaaaa
    21361 cttatagcag ctttcgagat gtttcctggt attggaagga aaatggcatc aagacttgct
    21421 ttttatcttc ttactatgga tgatgacagg attaagttca tatcagatgc gataaaagat
    21481 gcaaaagcag gattgtcata ttgttcgata tgttgcaata ttacccagaa tgacccttgt
    21541 gagatatgta aatcagacag ggatcattcc gttatatgtg ttgtttcatc agtaaaagat
    21601 cttatggcta tggaacggac atgcgaattt gatggtgtgt atcatgttct tcacggagtt
    21661 atttctccta tggagggagt taatcctgaa gatcttaaga ttaaggaatt gttaaaaagg
    21721 cttgataaag gtgttactga agttataatt gctactaatt cttctgttga aggagaagct
    21781 acagctatgt atctttcaag gcttattaaa ccatttgaca taaaagttac aaggatagct
    21841 catggtattt ctgtggggac agagatagaa tatgcagatg aagctaccct cataagagcg
    21901 ctaaagggga gaattgcaat ataaaaagca gattattgct atatttgtta caaaactgca
    21961 acaaatttag tattgcaatc ggcagaaatt tttaatataa tggcaaaagt gatattttag
    22021 agaaaggggc atacaatgat tagattagca gtaaatacag ttgcatttga tggcaaaggg
    22081 ataagtatgc ctgtgacatg ttcaaaatag tcgagtgaat atgttctgca ggcgaaatgg
    22141 cctgtggaat atttataaca tacatattat gtgtaaaccc tcaggtcgga aattatggta
    22201 gagggttatt tttttgaaaa aaataggatt attaaaacaa tttttaaaag gcaatggagt
    22261 cgcttatgta gcaggtctga tatttatgat aattggacag ctcttaagtt tggcaaatac
    22321 attcataata ggtgtagtaa ttgactctgt aataggtgag atactgccag agggaatagc
    22381 atacaagggg ctttctcttt tagggggtaa agagcacctt atgcaaaatc tatggatatg
    22441 cggtctggta ctggtattat tctcagtaat aaacggaata ttcatgtttc tgtcccgtaa
    22501 atactcagca attgccagtg aaggtatagc taagaagata cgaatggatc tttatgaaca
    22561 tatacagaat tcaacgtatc tgtataacgt gaatataaag acaggtgaca tgatgcaaag
    22621 atgcacttcg gatttggaaa cagtaagaaa ctttttagca acacagtttc aagacctgtt
    22681 aaggtgtata gttctcatta ttgctacagt atcaattatg agtaacatta atactactct
    22741 gacacttgca ggattttcac ttatcccgtt tattggtctt ggagctttct tcttctttaa
    22801 gtatgtgtca aaaatgtttc tattggcaga tgaagctgaa ggagctatgt caacagttat
    22861 agaggaaaat ctatcaggaa tgagaattgt aagatcatgt gcaactcaga gcagggaaac
    22921 tgaaaagttt gtcaaatctt caatagatta cagaaataag gatttgaaaa ttactgttgc
    22981 ttttgcatgg tattggggcc tttcagatgc aatatgccag atgcagatag gtatagttct
    23041 ggtaacaggt atatatcttg cattaagagg tgatgtgact gtaggaatgc ttactgtgtt
    23101 tatgacttat gttacccagc ttatctggcc tattcgagga atgggaagag tattgggaga
    23161 tatgggtaag gcaaatgtag ctcttggacg tataaaagaa gtacttgatg ctccaagaga
    23221 accaggtcgt gaagatgcag gtcttacacc aaaaatacag ggaagtatta catttgaaaa
    23281 tgtatcattt ggatatactt cagataagcc agtacttgaa gacatatctt ttgaaattga
    23341 aaaaggaaaa acgctggcta tattaggaag aaccggttct ggaaaatcta ctcttctgca
    23401 tctgcttgtc ggacttttac cttacaaaga gggaagtatc aaagttgatg atgttgagct
    23461 gagcgaaatc aataaaaggc atttgagaaa gaatataggt atagtgcttc aggaaccatt
    23521 cctgttctcg aaaacaataa aggacaacat ttcagataat gatccaagta ttactgatga
    23581 ggacatacaa agagctacta ccatagcttc agtagaccag gctataaaag gttttgaaaa
    23641 tggctacgaa acaatggttg gtgaaaaggg agtcacctta tcagggggac aaaaacagag
    23701 ggttgcgata gcaagaacat tgataaataa ttgtaagata ctgatttttg acgattcatt
    23761 gagtgctgtt gatatgcaga cagataagaa tataagaaat gcattaagaa agcactgttc
    23821 tgatactaca actattttga tatctcacag gataaatacc cttagacagg ctgatctcat
    23881 tcttgtaatg gaagatggcc atataactga tcgtggtacg cataatgagc ttattcaaag
    23941 agaaggactg tataaacagg tttatgatat gcaaagttgt gtactggaga tggaaaaggc
    24001 aggtgattaa taatggaaca agataacaaa gaaatattta ataaaagtat agatgtgaaa
    24061 ttatggaaaa aggtattgaa gtaccttggc aaatactgga agcatgttgt tggaatagta
    24121 atagcaatgg taatacaggc ggcaactgat gctacattcc cattatttac agaatatgcg
    24181 attgacaact tcatagctaa caagacttca gaaggaatag taaaattttc cattctgttc
    24241 gtcatactgc ttttactcaa aggtcttaca atatatatct ttatatattt ggcagacagg
    24301 gtatggcatc tggtaagtca tgatataagg gttgacggat tcaaaaaact tcaggagcta
    24361 agcttctcat actatgacag aaatgctaca ggcagccttt ttacaagaat tacatctgac
    24421 gtaggaagac ttacaggaat tatctcatgg gggcttgtag atatgatctg ggcaatcagt
    24481 atgattacga tgatgaccac attcatgttt atgaagaatg tgaagctaac tttggtagtt
    24541 cttactgtag ttccaattct gattgtaata tcaatattct ttcaaaaaag gatactgttt
    24601 tacagcaggg aggcaagaag atacaattca atcattacaa gtggcttttc agaaggtatc
    24661 cagggtgcaa ccacaactaa aacattggtt agggaagaat ctcatataaa tgaatttgag
    24721 aagaaaacgg atgatatgaa gaatgcagct ataaaaagcg cagtatattc aggattattc
    24781 tttccgatag tatcaatttt aggtattgtt ggaacttcct tagctgtatg gcaaggcgga
    24841 gtcatgatac tcggtgagat aataactgtt ggaacgctga ctttgtttat tatatacaca
    24901 ttgcagtttt tcaacccgat taacgatatg gcaagagtat ttgcaaactt ccagtcttca
    24961 caggctgcag gagagaggat aatgtctctt ttagaagcag atattgacat atcagattca
    25021 gatgaagtaa tagaaaaata cggtaaaata acatctgata tgcaggttcc caaacttaaa
    25081 ggttctataa ggtttgagaa tgtatccttc cagtataaag aaggcgagaa agtgcttgat
    25141 acattcaatc ttgatgttga accaggtcag tgcatagcgt tggtcggtga gacaggttca
    25201 ggaaagacaa caatagtcaa tcttgcgtgc aggttctatg agccgactga aggcgtgata
    25261 tatgttgatg gggaagatta taaaaagatt cctctgatgt atattcataa aaacttaggg
    25321 tatatgcttc aatcacctca tcttttcagc gggactataa tggataacat ccgttatgga
    25381 aaagaagatg caactgatga ggatgttatg aatgctgcga aaatggtaga ggctcatgat
    25441 ttcatcatga aattcgagga tgggtataac cacgtcatat ccaaaggtgg aggaggtata
    25501 tccaccgggc agaaacagct tatatgtatt gcaagagcca tagtgaacga cccttgtata
    25561 ttcgttcttg acgaagctac aagttctgtt gatacacaga ccgaaaaaat agtccaaaga
    25621 gcaatatcca aagtattgag caataggaca agtttcgtgg ttgcacacag gctttcaaca
    25681 atacgtgaag ctgataagat actggtaata gataaaggaa aggtaatcga gcagggaagc
    25741 catgatgagc tgatgatatt gaaaggtaac tactacaacc tatatatgaa ccagtttatt
    25801 gaagacagca acaaggacat ttttacaaat cctgagctga cagcggctga atgccaataa
    25861 gaaataatca cgcatctgta aaaagggtgc gtgtttttct atgtatatgt ttataaatat
    25921 gaaaggtaat tatgaatgag aataatgtgc atgagataaa aaaagaatca ctgatttcag
    25981 cgttaaataa taaattcgac aaaaaaattt ttgatgttga atatacagca gacagactct
    26041 gtaaaggtac agtagcagag gttattaaaa ttgaaggaaa tgcgaaaaac catgaatata
    26101 ttcttccttt caaattagtt ctcaaaatac agaaaaaatg gaacagacat cttgaccctt
    26161 tttgctggag acgtgaatac gacatatatt taaataagct tgataaagaa atatcaagat
    26221 ttataagact tccagaatgc tatcttcttg aagaaagtca tgatacaact aaaatatgga
    26281 tggaatatat tgaaggaatg agtggagaag atgatattga tgataagaag ctttctttag
    26341 ctgctgaaaa actaggcatg cttcaagctg attttcacaa atccggaagt tttgatcttc
    26401 catatattcg caattttcct gctgtggaat caagctttga tctttggtat aagcgcatag
    26461 aagaagtact aaagaaacct atatcagggt ttcctgacaa gataagagaa attctgaatc
    26521 aatatgcaga aaattcaaat aatttactag catcattcaa atcacttcca cttacattat
    26581 gccaaggaga tgttcaccat gacaatcttt tattcaaacc ggaaaatgac ggttttgata
    26641 tatatcttct ggactgggac agctcaggat atggatatat gggtgaggat gctattgatt
    26701 tgttacttga agcttttttg tataccaaaa gagatgtatc ttttatgaat gagtataaac
    26761 ataagatact ttcaggttat tgtaacggtg caaagaaatc acgaatggat tattttcttg
    26821 atgataaact tattacagat atatttgcat attcctgggg attcagggta acaagccatt
    26881 atctgtatta caaaacagat gatcttaaga aaagatgtgt agatatactg aatgtcatgt
    26941 ttggactgaa aatctaatca tcatcagcat ctagcgaaat attgaagcag tgagtctgca
    27001 gtgtggacaa tgctctgtac tgttctgcat acttgtattt tgtgcctttt gcagcaatct
    27061 tggcgagctc ttttacgaac attaaaagat tattgtcaac ctgagatttg tgacatttta
    27121 ctgaatccag ttttttatca atgtattttg atatatccac ttttcggttt ggcttgtctg
    27181 tataatagaa agccagaata ggtgtagtcc atatttcatg ccgagctttt ccatcttctg
    27241 aatagggata gaaatcgcac attgcatcaa gtactgcgta ttttactgct gtacctgttt
    27301 ttatatgatc gctatgacat tctgttgcaa gattggggtc aggggtaaat acggcatcag
    27361 gttttatttt ccttataatc gggagtatag cttgtgatat cttttcaact ggttcctgtg
    27421 ttttatcgca aaaccctaaa aaccctgcat tcttcacatt taaaaactgc tgagcttgca
    27481 gagcttctga ctgacgagta gtaggaccat ttttatcacg ttccagtaat ctgtcatcag
    27541 ttacagtaag ttcatatact tcagctcctg attcagctaa caaggccatc atacctccag
    27601 ctcctatctg attgtcatct gcatggggtt gaatgaaaag agctctttta atgcctttaa
    27661 gtttaggcgg ttttcttaaa tgtgatatta tcgggttatc catagtttac ctcgtattta
    27721 ataagatata tcttaatatg atatcaaaca gcttaaataa tgatttgatt atatcataaa
    27781 ctgtttatta gttcatatat agttcaatat ggtcatatat tatatgggta taaagctgaa
    27841 attaactaaa attaacagtt ttaaaggaga taaagcagat gaatgaaata tacgcggtta
    27901 caggagcatt agggtttcta ggaaatgcaa tcgtggacaa cctgctgtct aaaggaaaaa
    27961 cagttagggt tttagatata ggacaggata agcatgattt actaaaagga aagagtgtta
    28021 ctttttacaa aggagatata tgcttaacat catctttgga ggatttcttt aaagtagatg
    28081 atgatcaaca gctcataatg attcatgcag caggcatagt ttccatttca tctaaataca
    28141 gccagttaat gcatgatgta aatgttaaag ggacgcagaa tattgtagat atggctttga
    28201 agtataagat aaaaagattg gtttatgtaa gcagtgtaca tgctataaga gaacttccaa
    28261 aagatgaaac cataattgag actcttgatt ttaatcctga caaagtaaaa ggtgaataca
    28321 gtaaaagtaa agctatggct actaagatag tattagatag tgttgaaaaa ggtcttgatg
    28381 cggtagttgt tcatccctca ggaattatcg gacctggaga ttatgccggc ggtcatacaa
    28441 ctcaactggt aaaagactgt gctaatggga atcttacggc ttgtgttaat ggaggttatg
    28501 attttgttga tgtacgtgat gtagcagaag gtgttgtgtt agcagctgaa aaaggtataa
    28561 aaggaaatac atatatactt tctggtgagt attatccagt aaagaaaata actgatgcga
    28621 tatgctctgc tataggacgg aaacaaatta agaccattct acctctgtgg tttgcaaaaa
    28681 tgacagctcc ttttgcagaa ctttattaca gaattagaaa acagaagcca ctgtttaccc
    28741 gctattctct ttatacgctt cagagtaatt ctaaatttaa ttccaataaa gctcagaaag
    28801 agcttggtta taaaaagaag ttctcgttga agcaatctat agaagataca tatttgtggt
    28861 gtaaagcgaa taatcttata aaccaaaaaa agaaagctaa aggaaaagta taatagttct
    28921 atattgtata gtaagctaat accttaaagg tatctgattt atcaaccttt ataactaaag
    28981 ggccatttat tagcaaagga ttatcctttg ctttttcttt aagcataacg aaatatttac
    29041 accacacaag attctctgtc tttaccatgt tcattggatc atacccacat attgtgaaag
    29101 aaccttctct gcagttaggt gatttatcaa agaactcatc aaataacttc tgtctgtttt
    29161 ttagaaatgt ttttaaggag gtttctatcg tatctgtgtt tacagtttca ggtaaattac
    29221 ctgaagggat gctttcagat attaattcat aaacagtcct tttctctgtt gatatgtcat
    29281 gaaaaactga tatatctgta ttatgtaaag aaagcaaaaa caaagatcca gagaaatatg
    29341 ataatcttct attatcaaaa agtatgtgtt ctatatcagc aagattatta aaataattgt
    29401 ttatcttatc ggtatacttc tcagaatcaa gctgtttaag tgctaaaagc cctgcatatt
    29461 cggccatgcc ctcaacagtt tctatcctgt actcataatc aatatgatct ttaattaatt
    29521 cttttctttt gaatcttgat gtaataatat ttgatatgta ctcttttatt ttctttttat
    29581 ctttctcttt tacagctctt gcaatcagct gattttcagc atgctttaaa agaacatttt
    29641 caagtatgac gggataatcc aatgctaata tatcatttgg aaaccttttt tcacctaact
    29701 catactgata tgcatgaaac atttcatgta tcatatttgc agcaagttca attggatctt
    29761 cttttgaata atcatcaatt ttccatatcg ctatctgttc atcgtcatat tttatgctgg
    29821 tgttacctaa aaagcattct tcatacatga ttgttttgtc tttgaaatat acttttttat
    29881 catcataaag agcaaagtga tatgcatgaa atccttccca tatggattcg aaatccactt
    29941 tgcatatatg ctcataaacc ttatcgtata aaagtctcat aaattttacc tgatttctgt
    30001 ttgttttatt aatataagta taggataata tgagaggttt tacaaccagc ataaaaaaca
    30061 catttttgga gttttaataa ttaaggtatg actttttaaa tataataaca agaataaata
    30121 tcaggtcctg tttttgaaaa ctacaatttc tgcatcagtt ccgtcttttc catattcaca
    30181 tactaaaaga aatttctcat ctgcaacaat tccatagcat ctgctgctat tctttttttc
    30241 tagctggtaa ccaatataga ttttattatc atcaagaagt ctgattgttt ggccgcttgg
    30301 aagagtatac tcaaggttaa cataggaacc tttcagagca tttaactttg ttacttcttc
    30361 catatcttca atgccaagat tattgaattc ttctattaat ttttcctgaa aaatgcatga
    30421 gccttcagga cattcgctgc aaaagttttg tcccttatca taacagcact ttgcaattac
    30481 acagtcttta ccaaagggct gtccgtgtgt ggctatacat ccgccacatg tttttatata
    30541 tgaacatacc gagcagtcaa ttccacagat tgtattcaaa tcatattcct ccaatagatt
    30601 aattataatt atataaaatc attatagcat aaacttatat gctcaaacgt gatatagatt
    30661 gtgtaagtgt acactgcaat tttaatgatt tattgttctt attaaattaa aaaaacgtct
    30721 tctgttacat tgtacagaag acatctatat ctggtgaaga tgattggact tgaaccaacg
    30781 acctcttgca tgtcaagcaa gcgctctaac caactgagct acacctccgt gcctatatat
    30841 attacaatag accaagcaaa cctgcaatat taaatgtctg aagatacagt aagttttcac
    30901 tgtcacgcat tgagtcttca agacttatag ggcctctgct tatggaaaat agggaattga
    30961 tgccatagtc ttttagtact tcagcatctt ttgtaactga gcctgatata catattacag
    31021 gttttacata tttctttgaa agcatgccga ctctcatagg aagctttcca tatttagtct
    31081 gtgaatcagt acgaccttcc ccagttatga tgatgtcact attttttatt gtatcttcta
    31141 aatcagtata acttaaaata acatcaaatc cgctttttat aaccggcttg catactgtca
    31201 taagtccacc caccaatcct ccagctgcac cagaacctga aacattcgaa atatccttat
    31261 ttaacttttt tttgaagatt ttggcaagat tttctagacc tgcatcaagt tcaacaacct
    31321 gttctggagt agctcctttt tgagcagaga acacatatgc tgctccttct ttgccgtggt
    31381 atacattttc cacatcacag gcgaattcta tttgtgcatt ttctaattct ttaataatac
    31441 ttgttgagtc aatactgtcg acatttatca tttccgctcc gcaaggggct aatacagtgt
    31501 tattcttatc aaaaaatacc atgcctaatg cagctaatgc acccattcca ccatcagaag
    31561 tcgcagtgcc tccaaaacct acaatgattt ttctatatcc cttttccaaa gctgctctta
    31621 tcaactgtcc ggtaccatat gatgatgctt tcatagggtc aagtttattc tttggtattc
    31681 cctgaatacc tgatgcgcat gctgattcaa taacagctgt tttgttgtcg ccaagtattc
    31741 caatatatcc tgtcatttca tttccgaata cgccttttac agtaatatac tctttacgcc
    31801 ctctagttgc gaaaaccaga cagtcaactg ttccttcacc accatcagcc attgggtatt
    31861 ttagtatgtc acaatcaacc atagcgtcag ttaaggcttt tgctattatg tcacagcatg
    31921 ttattgcgct catgcaacct ttatatgaat ccattgctac tgctattttc atgtttatac
    31981 ctcgaaaacg aattttctta aattgttact taatcgttga ttccgctctc cacatgtcgg
    32041 cattgtatcc catcatattg aacagattat ctgtgcattc tataagcctg ttcatgatat
    32101 catcatcaag tttaagttca gctgcagata tattctgatt gagttggtca aagtttcttg
    32161 ctccgacaag tacggaagct atgccttctg tgtgcatgtc ataagcaata gataaagctg
    32221 acattgatac attcatagat tcagctattt tctcaatgca atcaatggtc ttgaataaat
    32281 cagagtgcag atgtgaaaac tcatcagaca gataccaaac ccttcttagt ctttcaggaa
    32341 tctggtctat atgtctgtat tttcccgtac ataatccctg agcaagagga ctgtagcata
    32401 atataccgat ttctttttct atacacaatg gccttatatc atgatctatt gctctaaaca
    32461 gtaaattata tgccatctga ttagtatgta taggtccgta tttggttatt tcatttaaat
    32521 cctgtactcc gaaattactt actcctattg ctctaatttt tccatctttg taaagcttta
    32581 ataatccttc cattgtttca caaaatggta cagttcttct tggccagtga atctggtata
    32641 tatcaatata gtcagtattc agtcttttca aactatcttc acatgcttta attattgttt
    32701 ctttgtcagg atgtgcatct gcagtttttg tcgctatgac aactttatct cttcttcctt
    32761 ttaatgcttt tcctaatact tcttcgcttt ttccattacc ataaactgct gcagtgtcaa
    32821 ttaatgttat acctgcatca attgctgcat gagttgtttt gattgataac tcgtcttcct
    32881 gtgaacccca tgtagtatca tttgaaaaga cccatgtccc taatgccatc tttgatacgg
    32941 tgatattagt atttttgatc tttatgtagt tcataagcta gctcctttaa atatgatata
    33001 tattattata ccatcatttc cattccgagt tccttcatga cttcattgta tctttcaaga
    33061 tatgcgttat cagtttcaat tccatgaaga tatctgtcag gactatctat gtcttttaat
    33121 aactctgcaa gcatatcagc tgaatgcatt tgtttattgc tatgctcaat tactgcttta
    33181 actatgatat taacttcatc atctgtaatt atcgatactt ttgttccggg accattaatg
    33241 aatctttcaa cggcttttct aatatatgat tctgcaattt ctgcgtgttt ttttcctcat
    33301 tgcctaaata gaaacaaggc agtatattct gaattatact gccttgtaat attgctcaga
    33361 aagtttattc tcctatctta tgcagtataa tgccttctcc tatatctgat ttgtttatgt
    33421 aataatcagg ggggtatttg taatttgagg aaattacaat aaaatcacca gtatcatcaa
    33481 tcatatttcc caaagtcgtt cctgtaaatt cgccgttata ggtatctaga agggtatact
    33541 gtttgtcaaa tatatataat gtctggctgt tactaaacag atcataacct ctgtcgtctt
    33601 ttgtattagt aaaagtaatg acatatatgt tatcctcata atagttggca actgatctcc
    33661 agcctccttc aatcactgaa tctgatactt tgttcatctg cttagtcgca agatcaaggg
    33721 tatagaagcc gtcattgtgt ttgtagcagt atcctttttc agcatcgtaa tagcagttag
    33781 tatattctgc ccatttatct gtcatatgtt caaatttatt atcttcaagt gacaacatgt
    33841 atagatcaaa atcaatggaa tctgatttta agttgtagta atctgtaggg ttaattcctg
    33901 ttttgtttac atagaagaat actttatctc caacaatcgt aaacatggag atttctgata
    33961 ttgcatcatc acgaaaaact ttttcatatt ccgacttgct atcaagttta atacgataaa
    34021 agttatcatc cttattgccg actgctccaa gagaacctcc ctcagttact aaaaagtaat
    34081 aatatccgtt atggaaacag ccatatgata tgtaacttgc catatcgtaa tcagctgcaa
    34141 tagtttttac gacttcatga ttttttccaa acatgtccat actcataagt ttcaaggtca
    34201 tatcactaac gaattcagta aaatatattt tatcgttata gtaaccgata tgagtattat
    34261 agcctatttc attacctaca aatgaatcac attcgttggt actatgatcg caatcttctt
    34321 ttgtgcacaa cttcttaaat tctgtatttc catattggga ataatataca aatcctccaa
    34381 tattttgata tttgccatat ggagtattta aataggaaaa ggctatttgt cctccctgat
    34441 tcatggatga gtaatattca tcgggttttg agttgtctgg ttttagtatt tttaaaccgc
    34501 ctgtgtttac gcacgaagac aacatgacag ataatagaag tacaatagtt atcattgcat
    34561 atagcttttt tctcattaca attaactcca atcatttaat tttagatgtg caaatatgca
    34621 catatctatt tatttatttt caacacggta agtatattga tactaacgaa ttttgtcaat
    34681 agttttaaca tagctcaaac aattgtacat acctatatct tatttaaaaa atatgataaa
    34741 ctaatcatgt tacggaggca gatatgggaa gatatatatt taagcaggct gaacctgtat
    34801 gggcaaacag gcttcaaaaa gaaatgaata tacgcatggg ctttatggca aaagtgggga
    34861 agtgtaaggc atctgtgaat cttgcctgta gcactaatta ccacatatat gttaatgaga
    34921 agtttatcgc ttctggtcct gcacgagctg cacacggata ctatagggta gatgagatag
    34981 atataactaa ttatctgatt aaagatgtaa atagtgtggc tgtgattgta tggggttact
    35041 gcgtaaacgc ctacgcagtt cttgaccagc cttctttttt aacttgtgaa attatatgtg
    35101 ataatgaagt aatagcagca acaggtgtaa agggctttaa tgcatatcta ttagatgatt
    35161 atgtaagaaa aacacaaaga tatacatatc aaagagcatt tattgaatca tatgttttaa
    35221 atactaattc aaatgattgg atgaaggact tgattattca gcaggctagt cttgaaatat
    35281 gtgaaaagaa ggaatatata acaaggaatg taaggtatcc tgaatatgag aagctctatc
    35341 ctttagatag tgttgttgta ggaaaacaca atcctattaa accagattca tacaggcttc
    35401 cctggcattt gaagattgaa gatgaaaact ataagcagtt taagacagaa gaacttgatg
    35461 aaaatatctg tgaaatgatg caacagattg aatatgatac ttctgaaaca gttataaaac
    35521 aatgtgcaaa tggattaaac ctttcaaaag gtgaatatgc tatttatgaa tttgaaagag
    35581 aagctactgg atttatttca tttaagataa attgtgaaga aacttgtgaa tttttaatga
    35641 tatatgatga gatgctttca gatggtatca ccaatccaat tcgtattcat ggcctgtggg
    35701 taaataaata tgttttagaa ccaggagaat atgacttagt tttttttgaa cctgtaagca
    35761 tgaaatattt aaatactgtt atgctaaagg gtaaagcgag tatttcgtca tttcatatga
    35821 ttgaatacaa gcatccacat atagataaat ctcttaatac atcagatcct gatttgcttg
    35881 aaatatttga tgcagctgtt gagacataca ggcagaattc actggatata tatatggatt
    35941 gcccttcaag ggaaagagcg ggatggttat gtgacagctt ctttacgtca agagttgaat
    36001 atttcctaac aggaaaatcc ttaattgaga agaacttttt agagaatttc ctattgccgg
    36061 aaacctttga taatctgcca aaaggaatgc tgcccatgtg ttatccgtca gatcacgtaa
    36121 atggtaactt tatacctaat tgggctatgt ggcttgtttt acagctatat gagtataaac
    36181 aaagaaccaa tgatgtgcag atgattaagg tattcaaacc taaagtttat ggtttattag
    36241 aatatttcaa aggttatata aataaagatg gcctacttca gaatcttgat aaatgggttt
    36301 ttgtagaatg gtcatttgcc aataagcttg tacaggatgt taactttcca tcaaatatgc
    36361 tttatgctct tatgctggaa agaatatcag ctctatatga tgatagcgat ttgctggttc
    36421 aggctgaaag tataaggcag agaattagaa aaatatcatt taatggggag ttctttgcag
    36481 atcgtatggt tatgactgac aatgccctca aagtaactga tgaatgtaca gaagtttgcc
    36541 agtattatgc attctttact aaaactgcga caaagaagct gtatccgaag ttgtggaaga
    36601 ctcttataaa agatttcgga cctgatcggg ttgaaaagaa actgcatcca ggcatatatc
    36661 ctgcaaatgc atttattggc acatatttaa ggatggaact attatctgag caaggtcttc
    36721 ataagcagct tttatctgaa tcagtaggat ttttcaagta tatggcagaa agaaccggta
    36781 ctttgtggga aaataaaacg gataatgcat cgttaaatca cggatttgcg tcccatatag
    36841 ctatatggct ggataaagct gtagaggcat tacagaaaaa agaatgaaca attccaaata
    36901 ttgtatacga aatgtattga caagttattt ttcgtgtggt ataactacat agtatctaaa
    36961 tgcattctat gaactcgcaa ggcttgtaaa atcattttta gtaagtggtt tcacgacctg
    37021 gggagttttt ttagttgcag taggtaaaat ttttttgtgg aggtaccaga tgaataacgg
    37081 tacagtaaaa tggttcaatt ctgagaaagg atatggtttc atatccaatg acgaaggcgg
    37141 agaagatgta tttgtgcatt tctcagcaat taacagtgat ggctacaagt ctctaaaaga
    37201 aggacaaaaa gttacatttg atgtagagca agatccaaaa aacagcaaaa agcttagagc
    37261 agccaatgtt tgcttagcat aagcaaacga agatctaaaa cgctgaccta atcatattaa
    37321 tatataagta atagagtaag aatgtgtttt gaagaacggt tatccgttct tttttttatc
    37381 ttcaaggtgt agtatacttt tgatatgaag aaatataact ataactgtag tactaaaagt
    37441 aaagtataat atatatatta aaatcatata gtaggaaaag gaaaaggaat gtttagaaac
    37501 aaaagtattg aagaatttat agaggtttta tcatcaaagg aacctgtgcc aggaggcgga
    37561 ggagcttctg catttgctgc atctatggga accgctcttg caagtatggt aggtaattta
    37621 actttgggaa agaagaagta tattgatgtt caggaagata ttaagatatt gcaggagaaa
    37681 accaaggttc ttactgatga gctaatgaat ttgatagacg aggatgctaa agtgttccta
    37741 ccgctttcaa aagcatattc tctgccaagt acaacagaag aagagaaaat atacaaagag
    37801 caggtaatgg aggaagcttt gaatattgcc agttcagttc cggtaaaaat tatggaaaaa
    37861 tctcatcaga tgcttttact gttagatgag tatgcagata aaggctctaa aatcgcaata
    37921 tctgatgtag cagtgtctgc agtaatgtta aaagctgcga taagtggagc atctgtcaat
    37981 gtttatataa acacgaaaat gatgaaagac aggactgtag cggataaatt aaacagctat
    38041 tgtgatgagc tgatacaaaa aggtacaaaa ctggctgatg aaatatttga aagggtagaa
    38101 aagaaactca ggtgatattg tggatattct atatggtaat gatgtagtaa aatcaatggc
    38161 cgaacaaatg aaaagacaac ttgaagctat tgttggttat atccctcatc ttgcgatagt
    38221 gagagtcggg gaaagattgg atgatttagc ttatgaaaga ggcgcagtaa aaagatgtga
    38281 gaaattagga ataagatgta ccgtatacag ttatccttca gatattggaa atcaggcatt
    38341 tgagtttgcg ttttctgata ttaatgctga tgatgatgta gacggcatac ttcttcttcg
    38401 tcctctgcca agacatattg atgaattggc aataactcaa atgattaacc cgattaagga
    38461 tgtagactgt atcagtccta ttaatattgc aaaagtattc tcatctgatg aatcagggtt
    38521 tgctccatgt acagcagaag cggtaatgga aatgatatct tatgcaggca ttgaccttaa
    38581 gggtaaaaga gcaacggtag taggaagatc aatggttatc ggaaaaccag ttgcaatgat
    38641 gcttatgaaa aagaatgcaa cagttactgt ctgccataca aggacggttg acatggaggg
    38701 tacttgtaaa aatgctgaag ttttaatagc tgcagcaggc agagcgaaaa tgatcaacag
    38761 cagttatgtt gcaaaaggtg cttatgtatt tgatgtaggt ataaatgttg atgattctgg
    38821 taatatgtgt ggtgatgtgg atattgatga tatctcgtct cttgcacagc tttgtacacc
    38881 tgtaccaaaa ggagtaggaa cagtgactac atcagtttta gccaatcatg taataaaagc
    38941 agcaatgctt cgaagaggta atgtatgata acatttgaag atgtaaagaa gaatgaagag
    39001 attaaaacat atataactaa agcagatgag tccttaattg ctttaggcta cactgaacat
    39061 agttttgcgc atgttatgaa agtagcaact acagctgaat atattctgac tactcttggg
    39121 tatccgcaaa aagacataga gcttgctcag attgcatgtt atctgcatga tatagggaat
    39181 ctcataaaca gggaagacca tgctcaaagc agtgcgcttt tagcatttag aatactaaaa
    39241 gacatgggtg cttcaccaga tgagatagcg actgttgtta ctgctattgg aaatcacgat
    39301 gaatccactg cgtttgctgt aaattctgtt gcagccgctt taatacttgc agataagact
    39361 gatgttagaa gaaccagagt aagaaacagt gatacatcaa attttgatat acatgacagg
    39421 gtgaattatt ctgttaagaa atctacagtt cgaataaatg aagatcaaac agagataagc
    39481 ttaagtctga ctatagaccc taaattttat tctgcacttg agtattttga aatattttta
    39541 ggtagaatga ttttatgtag gaaagctgca gagaagctag gtcttaaatt caggatgaat
    39601 attaataatc agcagatttt gtaagtattt tttgagatat atttttgaag ctatttatat
    39661 agcttctttt ttttctttaa aacagagaac ttctttttat ttacaacaat aagaaccata
    39721 tactaattat taccgactta atttaaatat atagctcatt acaggaacac taaacctgtt
    39781 tgctatcatg tcgcatttcg cttttaatag gaaatatact tttctagagt aatcttcaag
    39841 cccttcgtag ttgccctgtc ctttgcttat aactatatct gccttatcga atatatccat
    39901 aaattcatct gaacagtctt ttaatacggt tccaggcgtc atgcatcctg aagatataag
    39961 agttgaacag ttattcagtc ctgcgctttt tgcatctgaa agtgtcgcat cgtttattat
    40021 aggagcgtgt cttgtcgcgt aatacacttt taaattttca ggaaggcttt ctataaatac
    40081 tctgtcaaat accgcttctc ctgcattgtc gccgagtatc agaagtgttt tagctttaac
    40141 aatatctttc ttgaaaattt caatatcgca tattgtaaaa tccatatcca gctgatggtc
    40201 tatacattcc attacatcca aatccttaaa tattgctgaa tccattatgt ttcctattgc
    40261 ggatattttt aaagcagtat agaggtcatt gtttttgtca ttaagtattt ttttaagata
    40321 aggataaagt ttcaaagcac ttagtatatc cttcttcttt atatcttcat atggatcatt
    40381 attgcaagta agcattttta ctgtattatg catatgataa gccagttcag gtgaactatt
    40441 gtaatcagta aaatttgaaa taagtatagc gcatttttta agaatttcct gttgaatatt
    40501 tagattatct gtagccattt ttgctgcttc ataagcctgt ctcattaggc agggaacgca
    40561 ttcgttaaaa atcttcatta atttccgcct tcaatcatta atttaataca tagtatgata
    40621 tcacatattt ttactttgtt tagataactc tcaaatactt atgctttcaa ttatttatat
    40681 tgatggaaaa ttctgataaa ttaagtgatt ttgatgtatt cctatttttt cttttcattg
    40741 atataataaa ataaatagag ggggtatcta tatgaagaaa attaaagtac ttagcagttt
    40801 tgtggtttta gttcttatag cagttatgct gtcatcatgc agttttacaa ctgcgaattt
    40861 ctcaaatcta accatgtcta caggggtgga cggttttaaa cctgtcaatg caacaaccaa
    40921 atttacaacg actacaccgg ctttttatgt aacaggtgat attaacaatg ctcctgatga
    40981 tacagtcatt tcagcagtct ggtactatct tgatactgat tctgactata tgatagacca
    41041 gagccagtat caggttgaag gaacaaacaa ctctttctac ttttctttat caattccaga
    41101 taacggatgg cctgtgggaa catatcgtgt tgatctgtat attgatgatg ctgttgacca
    41161 gtcgatattc tttacggttg aataagtagt gcagaacaag atataattga atagaagaaa
    41221 tatcaaggag gcagcagatg aataatcttt tgcatttgaa gtatgctgtg gaagtggaga
    41281 aaaccggttc catatctaaa gccgctcaca atctgtattt aaatcagcct caggtttcaa
    41341 aggcaatcag ggaactggag acttctattg gtatcactat ttttgatcgt acccctatgg
    41401 gtgtttatcc tacaaaaaaa ggtgctgagt ttttatcata tgcaaagaat attttagctc
    41461 agcttgaagc tatggaatca cttagcaggc cggaatcaaa aaaagggcag atacttagtg
    41521 tttcagttcc aagagcaagt tatgtttcca gtgcttttac cagttttctt aatgatttag
    41581 ataaagattc tgggatgaat atagattaca gggagactca ttcggtcagg gcaatgaaga
    41641 gcgtatctga ggggagtaat gatctgggaa ttatacggtg ccagatgcgt ttcgagggat
    41701 attttttaac agctttacag aaatatgctc taaaacatga agacatattt gaatttgagt
    41761 acttactatt aatgtctaaa gatcaccctt tggcaaaaca aaaagaagtt aattacaaag
    41821 acttactgga ttttactgaa gtggctcacg gagatatttc agtaccttct cttccttcaa
    41881 taaatttacc tgataagtcg aaagtattca aaaacagaat tgcgatatat gaaagagcca
    41941 gccaactaga gatattaaac agaataccat atacatatat gtgggtttct ccaatgccaa
    42001 aagatgtttt agataaattt gaattagttg agaagaaatg ctctgtgcca aagaataaat
    42061 acaaagacat gttcatatat agagaaggtt atgttttcag tgattatgac aaaattttta
    42121 tggagaaagt aagagaatcc attagtaaag tatctttaat gattaaatag ataaggttat
    42181 atcgataatg gaatatcgat attccaaaat cgattctacc caaaaccata tatccgtcat
    42241 atcatataca tgtaaaagga atttacgtgt aaaggagacg aaaattatgg caagatttac
    42301 attaccaaga gacatatacc atggaaggga ctccttagag tcattaaaaa ctttggaagg
    42361 atccaaagct attgttgtaa ctggcggaag cagtatgaga cgtgctggtt ttttggataa
    42421 ggtagtagaa tatcttaacc aggcaggaat gcaggtcttg ctatttgaag gtgttgaacc
    42481 agacccttcg gtggagacag ttttaagagg tgcagaagcc atgaaggaat ttcagccaga
    42541 ctggatagta tccatcggag ggggctctcc aatagatgca gctaaagcaa tgtgggcatt
    42601 ttatgaatat cctgaaacaa cttttgaaca gttatgtata ccattcaatt ttcctaaatt
    42661 aagaacaaaa gcaaagttct gcgctattcc atcaacatct ggaactgcta ccgaggtaac
    42721 tgccttctca gttataacag attacgaaaa aggaattaag tatccgctgg cagattttaa
    42781 tataacacca gatgtcgcta tagtagatcc tcaaatagca cagacaatgc caaagaaact
    42841 gactgcatac acaggaatgg acgctttgac tcatgctatt gaagcatatg tatctacgct
    42901 gaacagtgac tatactgacc ctttagctct tcatgcgatt aaaatgatta atgaatatct
    42961 tgttgattca tataatggcg atataacagc tcgtgagaag atgcataatg ctcagtgtct
    43021 tgctggtatg gcattttcaa atgcattact tggaatagtg cattcaatgg ctcacaagac
    43081 aggtgctgca ttttcaggcg gacatatagt acatggatgc gccaatgcaa tgtatcttcc
    43141 aaaggtaatt aaatttaatg caaaaaatcc aatagcactt gagcgttatg ctcagatagc
    43201 taactttata ggtttaaaag gaagaaatga tgaaaaactt atggaagctt tgatagaaag
    43261 aataagagaa ttaaatactg ttcttaatat acctatgtgc attaaggatt atgaaaacgg
    43321 tataatagat gaaaaggaat ttcttgataa acttgataat gtatcacaat tagctatatc
    43381 tgatgcatgc acagggtcaa atcctcggag catagataaa gagcagatga aaaaattatt
    43441 gcttgcctgc tattatgact cagaagtaaa tttttaaagg agattgagat gaattacgta
    43501 tcacggcaga cattggaaag attacctgta tacttaaggt ttttaaaatc gcaatgtata
    43561 gacggtccca taaacacatc agcaaaaaca atagctgaag ctctgagttt aacagagata
    43621 caggtgagaa aagaccttgc cgcgataagc gacggcggaa aaccgagaat agggtatata
    43681 acaaaggatc ttattaatga tttagaagat ttcttgggat ataaggatgt aaatgatgca
    43741 ataatcgtcg gagctggaaa actcggcaaa gctcttatgt catacaaagg gtttgcggaa
    43801 tatggtctga acatagttgc agggttcgat acatcagatg aagctgttgg aactgatgaa
    43861 gaaggtaaac caatatttca tttagacaga atggaagagc tttgcagaag gatgaatatc
    43921 cgtatcggaa ttattacggc accggctgat tatgctcaaa gaattgcaga caaaatggtt
    43981 aagagtggaa tacttgcaat ctggaatttt gcacctgcac atctggaggt accagataat
    44041 gttctagtca gaaatgaaaa tatggcagca tcactggcag ttttatcgaa acatttaagt
    44101 gagcagttaa tgaactcata aggagataat atatgaataa aacaagagaa ataatagacg
    44161 gcacagagaa attaaatgct gctcttgata ggcttagggc cgcacagaga aaatttgcga
    44221 catatacgca aaaacaagtc gatgaaattt tccttgcagc agctacagct gcgaataagc
    44281 agaggataga attagctaaa atggcggtag aagagacatc tatgggagtt gttgaagata
    44341 aggttataaa gaaccattat gcttctgagt atatatacaa tgcttacaga gatacagtta
    44401 cctgtggtgt tgttgaagaa gacagagcat tcggcataaa gaaggttttt gaacctgttg
    44461 gtatactggc tgctgtaata cctaccacaa atcctacatc aacagcaata ttcaagactt
    44521 taatatcttt aaagactaga aatggtataa taatcagccc acatccaaga gcaaagggat
    44581 gtaccatagc tgcagcaaaa acagttcttg aagcggctgt aaaagcagga gctcctgaag
    44641 gaattattga gtggattgac ataccaagcc ttgagatgac taatatagta atgaaagaat
    44701 gcgatatgat tttagctacc ggaggacctg gaatggttaa agcagcttat tcatcaggca
    44761 aacctgcagt aggtgttgga gcaggtaata cccctgcaat aattgatgaa agtgctgata
    44821 tcctgttagc agttaattcc ataatccatt caaagacatt tgataatgga atgatatgcg
    44881 catcagagca atctgttata gtaatggaaa aagtttacga caaggtgaaa aaagaattca
    44941 aagaccgtaa ctgctatttc ttgaataaag aagaaactga gaaagtcaga aagaccataa
    45001 ttataaatgg aggccttaat gctaaaattg ttggacaaag cgcatttaag atagcagctc
    45061 ttgccaatgt agtcgttcca gaaagtacaa agatacttat tggcgaggtt acctcagttg
    45121 aactttctga ggaattcgct catgaaaaat tatcaccagt gcttgccatg tataaggcta
    45181 aggattttga agatgcctta tcaaaggcag agcatttagt agcagatgga ggttatggtc
    45241 atacttcatc agtatatctt gatacattaa gagcaaaagt taaattagac cagttcatag
    45301 accgcatgaa aacatgcaga gtcttaataa acactccatc ttctcatggc ggaataggtg
    45361 atttatataa cttcaaactt acaccttctt taacattagg gtgtggttca tggggcggaa
    45421 attcagttag tgagaatgta ggtgttaaac atcttgttaa tatcaagatg gtagcagaca
    45481 ggagggaaaa catgttgtgg ttcaggacac ctgagaaagt atatattaag aaaggatgcc
    45541 ttcctgttgc tttggatgaa ttaaagaatg taatgggtaa gaaaaaggca ttcatagtta
    45601 cagataattt cctttacagt aacggatata caagacctgt aactgataaa ttagatgaaa
    45661 tgggaataca gcatacagtc ttttttgatg ttgcgccaga cccttcatta gcatgtgcta
    45721 aagaaggcgc gaaagcaatg agagctttta atcctgactg cataattgct ttaggcggag
    45781 gttctgcaat ggatgcagca aagattatgt gggttatgta tgagcatcct gaagctgact
    45841 ttatggatat ggcaatgaga ttcgtagata tacgtaaaag agtatatacc ttccccaaaa
    45901 tgggagagaa agcatatttt atagcaattc cgacaactgc gggaacagga tctgaggtaa
    45961 cgccttttgc agtaataaca gatgagacta caaatgttaa gtatcctctg gctgattacg
    46021 aactgatgcc gaacatggca atcgttgatg cagatcttat gatgaatgcg ccaaaaggac
    46081 ttacagcagc atctggaatt gacgcagtaa cacatgctct tgaagcatat gcatccatgt
    46141 tagccacaga ctatacagat gctcttgcac ttaagtcttt aaaagtggta ttcgagtatc
    46201 ttccaaaagc atatgatgac ggaccgaatg atcctaaggc aagagaaaaa atggctgatg
    46261 gtgctactat ggcaggaatg gcctttgcta atgcgttctt gggagtatgc cattcaatgg
    46321 ctcacaaact tggagctttc catcatcttc cacacggtgt tgcaaatgca cttatgattg
    46381 atgaagtatt aagatttaat gcatcggaaa aacctgtaaa gatgggaaca ttctcgcaat
    46441 atggccatcc tcacacatta caaagatatt cagaaattgc agactatctt ggtataaaag
    46501 gaaagacaga taaggaaaaa ctggataacc ttatagcagc tcttgacgag ttaaaagcta
    46561 aagtcggaat taagagccgt ataatggatt acaacataga tgaaaaagac tttctaaaca
    46621 gattagatga aatgaccgag caggcatttg atgatcagtg tacaggtgca aatccaagat
    46681 atccgcttat gagcgagata aagaaaatgt atctaaatgc atattatggc aaacaggagg
    46741 aagtgtgatg aaactggaca agataattgc ggtaagaaca actaaaaccg tataccgtga
    46801 caatgacaag acaataaaag ttttcaatga agattactcg aaagccgata ttcttaatga
    46861 agcccttaat caggccagag tagaggagac atctcttcat atcccaaaga tactggaggt
    46921 taccaagatt gaaaacaaat gggctatagt atctgagtat atacaaggaa aaactttagc
    46981 acagataatg gaagaggata aaagcaggtt tgatgagtat ttagaaaggt ttgttgatat
    47041 acagattgag atgcattctc ataaagtacc tcttttgaat aaattaaaag ataaaatgaa
    47101 ccgcaagata tccgaatcaa cattagatgc aactacaagg tacgatctgc ataccaggct
    47161 ggaatctatg ccaaaaggaa ctaaattatg ccatggagac tttaatccta gtaacataat
    47221 tataacgaaa gatgatatac cttacataat agactggtct catgcgacaa tgggaaatgc
    47281 atcagctgat gtagcaagaa catacctttt attctggctt tcaggtgata ttaacggagc
    47341 agaaaaatac ttagacctgt tctgcaagaa aagtgatact gcaaaacagt atgtacagaa
    47401 gtggatacct atagttgctg cttcacagtc tgtaaaggga aaaccagaag aacgcgaatt
    47461 tctattacac tgggtcaatg tagtagatta tcaatgatga atataaatat taaataacga
    47521 ggagaaaagg tatgaaaata acagtatgta taggaagctc atgtcattta aaaggatcaa
    47581 ggcaggtagt ggaatcatta cagtttttaa tcagtgaaaa tgatttgaag gacaagatag
    47641 acttatcggg aacattctgt atgaataact gccaaaacgg agtatgtgta actgttgatg
    47701 gaaaaatgta ttcgctgact ccggaaacta caaaaagttt ttttgaaaca gaagttctta
    47761 ccaaattgaa atagttagaa gaaagaggtg gagaagatga cggaattttt aagatttaaa
    47821 cagtcaaact gcaagaactg ctataaatgc ataaggcatt gtccggtaaa atctattagg
    47881 ttttccggta atcaggcgca tgtaataggt gatgagtgta ttctttgcgg tcaatgtttc
    47941 gtcgtctgtc cgcaaaatgc aaaggaaatt gcagataaca cagaaattgc aaaagtgctt
    48001 atatcatcag gacagaaagt tatagcgagc atagctcctt cctttattgc aaactacaat
    48061 ggtataggta taaagtcgct tgataaagca ttgagaaccc ttgggtttta tgcagcagaa
    48121 gaaactgcaa taggagcaaa actggtaaag gataaatatg atgaaatgct caagcagggt
    48181 gacaaagata ttataatcac atcctgctgc cacagtgtaa atcttctaat acagaagcat
    48241 catccgaaag ctttgccata tcttgctgat gtattaagtc cgatgcaggc tcatagtatg
    48301 gatatcaaaa gaagatatcc tggatccaaa acagtgttta tcgggccctg tcttgctaag
    48361 atggatgaag ctgacagata tgcagggata actgactgcg tactgacctt tgatgacctg
    48421 acaagatggc ttgaaaagga agatataagg cttgaagaaa ttcctgacga agatcagttc
    48481 agcaaaacac gtctcttccc tacaacaggt ggcattttaa aatccatgtt atgcgagcag
    48541 gataactaca cttacatagc tattgatggg attgaaaact gtaaagcagc tctaaaagat
    48601 attgaagaag gcggtctgca taaatgtttt atagaaatgt ctgcttgtac cggttcctgt
    48661 ataggaggtc cggtaatgga aaagcatcat gctacacctc ttaaaggcta taaggaagtt
    48721 aacagatatg caggtaaaga agatttcaag gtagagcctt tagagtctca gataatgaag
    48781 aaaaatcatt tgcctattat tattaaaaat gatatgcctt ctgaaaatga gattgtaaat
    48841 attttagctc aaatgggaaa gacaaaacct gaagatgaac taaattgcgg atcatgcgga
    48901 tacgattcct gtcgtgaaaa agcaatagcg gtatatcagg gtaaggctga ctatactatg
    48961 tgtcttccat ttctgcaggc aagatcacaa agcttttctg acaccattat cacaaatact
    49021 ccaaatggca tattcgtact gaatgacaat cttgaagtcc agcagataaa taaagctgca
    49081 ttgtcattaa tgaggataag aagcgcatct gatgtacttg gggaacctgt tgtaaggata
    49141 cttgacccca aagactttat aaccgttaaa acaacgggag taagcataaa agataaaact
    49201 gtttatcttg ccgaatacca aaaatatgtt gatatgacag taatatacga cagagaatac
    49261 aagataattc tttgcattat gcgtgatgtt actgaagaat acaagataaa ggcaaaaaag
    49321 gaaatagtca acaagcaaac tgttgagaca gctgacaaag ttgttgataa gcagatgaga
    49381 gttgtacagg aaatcgcatc tttattagga gaaactgtag cagagaccaa gatagcgctt
    49441 accaaactta aggagacgat atcagatgaa taatctttgt gcagatatag ggtataacag
    49501 cctgaataaa tacaaagagc agttgtgcgg agatcacata gagtgtatta accaggatga
    49561 tgggtctaca gtgatagttc ttgctgacgg gttaggtagc ggcgtgaaag ctaatatact
    49621 ttccactctt actgcaaaaa tcatttcaac tatgatggct gaaagctgcg ggattgatga
    49681 atgtgtttca actattgctg caacattgcc tgtctgcgga gtaagaggaa ttgcatattc
    49741 aacatttact ataatacgta tagtaaataa taattatgca gaaataattc aatatgataa
    49801 ccctcatgta atacttctta gagatggaaa tagtgttgaa atgccaaaaa cactattaag
    49861 tattgataat aaagctattt actattccaa aatagcttta caggagaatg atatatttat
    49921 tgcaatgagt gatggagcaa ttcatgcagg tgtaggtatg agcctgaatt tcggatggca
    49981 aagagatgac ataattaaat ttatggaaaa actttatcat gtaggtttta ccgcgaaaac
    50041 tctcactacc atactgcttg atgaatgtta cagtctgtat gggggccagc ccggagatga
    50101 tacaacagtc tgcactataa ggataagaaa acgtgagcct atgaatctta tcataggtcc
    50161 tcctttcaat aaagatgact gttccaagat gatgtcactg ttcttttcaa aagaaggaaa
    50221 acatattgtc tgcggtggta ctacatctaa gattgcagct gattttttgg gaaaaacact
    50281 tatcccaagc cttaatttcg tagatcctga aatacctccg actgctaaaa ttgagggagt
    50341 ggaccttgtt acagaaggtg taatcactat taataaggtt ttgacatatg cacaagatta
    50401 tctgaaggat aacgagtcat acaggcactg gagctacaag aaggacggag catcacagat
    50461 tgcaaggctt ttgttcgaag aagcaacaga cattaatttt tatgtaggga aggcagttaa
    50521 cccagcacat cagaatcctg atctgccaat acatttcaat ataaagatgc agctcatatc
    50581 agaactggca gaatgcttaa agaaaatggg caagaatata aaggtcagct atttttaaag
    50641 gaggaagctt ttgagaaggt ttgatacgaa agttcannnn nnnnnnnnnn nnnnnnnnnn
    50701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    50761 nnnnnnnnnn nnnnnnattc aatgcaataa cttttgacaa tagacagaaa gcccatatag
    50821 ataaaacaaa atgtgtagaa tgcgggatgt gcgctaaagt ctgtccgtat aatgccattt
    50881 taaataataa aagaccttgc gagaatgcat gtaaagttaa agctatatcc atgaatgaac
    50941 acagggctgc aattatagat aatgataaat gtactgcgtg tggtgcatgt gtttatcaat
    51001 gcccgtttgg agcgattacg gacaaatcat tcattttaaa tgtaatagat atattaaaaa
    51061 agagcagtga taataaaaaa tataaagttt atgcagttgt agcaccttca atatccagcc
    51121 agttttcata tgcgaaactc ggtcaggtta tttccggcat aaagaaactg ggctttcata
    51181 cagttgtaga agctgcttta ggagctgata tggtagctaa tgaagaagct aaggaattat
    51241 atgaaaaaga gtttttgaca agttcatgct gtcctgcatt cgtgtcatat gttgagcaca
    51301 gttttccaaa cctcataaag catatatcac acaacttgtc ccctatggcg actattgcaa
    51361 aatacataaa agacagagag aatggtgcaa aggttgtatt cattggacca tgtactgcaa
    51421 agaagcagga gtttcaaaaa gaacatgttc ataaatatgt tgacagtgta ctgaccttcg
    51481 aagaactgca agcgcttttt gacagtaagg atatagaaat aatggcattg gatgaagatg
    51541 tgcttgataa tgcttcatat tatggaagga tatttgcccg ttcaggtgga ctggctgatg
    51601 ctataaggca ggctattaag gaaaataatc ttgatgaaag caaatataat ccattatcct
    51661 gtgatggtat agaagaatgc agagcagcct tattgaaagc agatagaaat atattgcaga
    51721 ataactttat agaaggaatg gcttgtgttg gaggatgtat cggaggagca gggtgtctta
    51781 cgcatggaac aaaagacaag acagaagttg acaggtatgg tatgcaggct tatgaaaaga
    51841 aaatatcaga tgctataacc atgataaaaa catagataaa tacttcaaat ttaccccggc
    51901 aaagcccgtt gtttcttaat agaacaacgg gttttgtgtt ttgttaatcg tatgaagaat
    51961 cgtcatcatc agaatcttct agactatttg attttgttct aaacattgaa gaagggatat
    52021 atttctggtc ttgatcaaat tgatccatac aatagaaata ttccccttca cacattaccc
    52081 atttttcagc tttttccata tcagcctcca atattgctat cagcacttaa tgatgtcatc
    52141 tgtaaaacat aaataaaata tcagatactt atatgataca cctttagtag accttttgga
    52201 atagagttaa tgatttttca catgaactat tatagtaatg gtaatattgt ctatactaat
    52261 caatatttaa atgcttagga agggatgcta ttacaatgcg tttatcttct ttgtcgaata
    52321 tgaaatatat ccgaccttca gatccaagat ataagtggcg gtctatttct gctttcacta
    52381 aactgccgtc ttttttattg taagtgaact cataatcctt cgtattagtt atttttggag
    52441 gatttacttt tgttatttca atgtagctgt tgtaaacttt cgagcatgtg tcatttctta
    52501 tatcctcagt tatttctcca cataactgtt catagtaatc tagcgcaaga tgctcaattg
    52561 catcacaaat aaaatcaaaa tcatattcat tttttatttt ttcaatttca tcgcaagcac
    52621 gtttgcataa tgtaagcctg ttgagaaaat atgtttctac ccattgagga atgtctttga
    52681 attttttagg tctgtttctt aaaaactgta tgcgcttaga tatatattta attttttctt
    52741 cgcattcttc aaattccgtt tcgatttctt ttatatattc tctattttcg tcaagttttt
    52801 ctttatactt tcttatttct tcatctttag cattaagttt ctggctgttt tctgcaatta
    52861 aattctcttt ttccgcatta aaactctttt ttaaatattc gtaatcatta ttgcttgatt
    52921 caagctgtct aagcagttct tctttgcttc tttttgtttt taaaaattcc atcagactgt
    52981 tttttctaag gtcaggaacg aattctactc cattaaaatc aacatctttc ttctcatagt
    53041 atttcttcac tcttttatat actgaatcct ttacattaat atcagatata aagtctgcca
    53101 tattgtatgt atctgttttt gcatctttat atttgtttcc gtcaataata aacaaatcat
    53161 tattatcata atcactatct gtatctgtta aattactatt tttagatatt aattgaagaa
    53221 gcatgtgtct ttttgtgtga ggtatgaaaa agaaaaatgc atatgctctt aagctgtatg
    53281 caagcttatt aaatacttct gctatttctt gtaagtcagg taatgattct ttcttttctt
    53341 ctttttctat ttcattatct tcattatcct tttctttttc attttcttta tcaatattct
    53401 cagattcagg tttttgtaaa tgtataacag caagcgactg tctttgagcg gatgttatcc
    53461 attttgcaag attctcaata taatcttt
//
LOCUS       DAOSXM010000006        53026 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2111
            NODE_401_length_53026_cov_11.630326, whole genome shotgun sequence.
ACCESSION   DAOSXM010000006 DAOSXM010000000
VERSION     DAOSXM010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864178
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 53026)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 53026)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:24:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,261
            CDSs (total)                      :: 2,229
            Genes (coding)                    :: 2,218
            CDSs (with protein)               :: 2,218
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 3 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53026
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_401_length_53026_cov_11.630326"
                     /isolate="AMR_MDS_2111"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            74..964
                     /locus_tag="PLQ68_01800"
     CDS             74..964
                     /locus_tag="PLQ68_01800"
                     /inference="COORDINATES: protein motif:HMM:NF025879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HXK71411.1"
                     /translation="MNYYDRIQKSIEFIERYIDESIDIQDAADISKMSYSEYCRFFHA
                     ITGYNVQEYIGKRKTAYAVNDMLKGKTDINYLAKKYCFSSTEAFCMIFRSNTGYLPSE
                     FLDQDIYYSFEMIDVRKIYYDTQDKMLIERFPDIRLLRPFPKMRMASYTSFGNQPENK
                     ACKVIMEYAKANKYLDSYKAFRIFGFNVSDSIKHDGTYGYTVCMTVDDSHEFSDGPIK
                     ELIIENGQYVVATTTVNNLVDTWERFKEWLRVSGMNSGDHQYLEEHLNCCNWRVEEPV
                     NDTEINLYMPVSRNSNNSKR"
     gene            1074..1553
                     /gene="tsaE"
                     /locus_tag="PLQ68_01805"
     CDS             1074..1553
                     /gene="tsaE"
                     /locus_tag="PLQ68_01805"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HXK71412.1"
                     /translation="MTKTYVIATGNELDTIELGKVMAAVFENRVVAVSGDLGAGKTHL
                     AKGFALGLGIEKHITSPTFNIMNVYTKNDKTFYHIDAYRLTDPDMIYDIGIEEALEPK
                     GITLIEWAENVEHILNKGYVKIYIEKTDDENKRLMFITADKESIDRMAKIIYEDTVR"
     gene            1534..2238
                     /gene="tsaB"
                     /locus_tag="PLQ68_01810"
     CDS             1534..2238
                     /gene="tsaB"
                     /locus_tag="PLQ68_01810"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HXK71413.1"
                     /translation="MKILLGDTCLKTASAAISEKNKLIAYMHSEGHTPHSVMFPKLVE
                     QVINTAQLQFSDLDAIACVNGPGSFTGQRIGLTYMKTLAYTLDKPFYTLNTLDVLANS
                     QKAKDDVLSLPLIDARNNEVFTAMYMNKDGFYINITPYSALHIDKVVEMTVKMCEKND
                     NLVVMLSGDAQKKHIGIFKNALDKKIMDYSAERDNVDLTRAPLLLTEENREDPFLAQP
                     FYLRESGAVRMKKIKE"
     gene            2235..2678
                     /gene="rimI"
                     /locus_tag="PLQ68_01815"
     CDS             2235..2678
                     /gene="rimI"
                     /locus_tag="PLQ68_01815"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012371661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HXK71414.1"
                     /translation="MKVTVRKAVCEDIDGIFKIEKSSFTNNWSYESLYEDICKTPISY
                     YLVAETEGNILGFIGMWHVMDEAHIMNVAVDSGFRHLGIGSMLLSTLIEYAEDCGILR
                     MTLEVREHNYNAISLYEKYGFLKYGKRKQYYQDTFEDAIIMWKEL"
     gene            2679..4460
                     /gene="uvrC"
                     /locus_tag="PLQ68_01820"
     CDS             2679..4460
                     /gene="uvrC"
                     /locus_tag="PLQ68_01820"
                     /inference="COORDINATES: protein motif:HMM:TIGR00194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HXK71415.1"
                     /translation="MPRFNEKIEYLLNNLPEKPGIYLMKNAEGETIYVGKAVNLKNRV
                     SSYFTNLSGHSAKTRKMVNSIDSFEYMVLDNERQAFITESDLIKSLRPKFNILMKDDK
                     SYPYIKVTVNEKFPGIYVTRDKTDKFARYFGPYPSKVSKKNTITLLQNAFPLRSCKKM
                     AKRPCLNYEMNLCLAPCSLECDVDKYNQTVTSVISFLSGESQKVIDDYKYKMQNASDN
                     LDFEKAAYFRDRINALTMISDNYALATDQNSVDVIAYAEKKDYISFFIMNIRKGKMSD
                     TRVINIKQERIEDTDIGYEFLRQFYDNNIPKNIIMYNEISNREDIEDWLSENAKSKVK
                     ITVPKKGKFKKLADTAYENALVALNLYMGQIKNMADVSPVYSLASFLQLETVPEIIEA
                     FDISHLSGQDAVASKVVFKEGMPYKKGYRRYIITNSNSRSDTDSLKEVLERRMKKGDL
                     PDLFLIDGGKNQVSAVKQIVNNEVEVAGMVKDDSHKTKALIYNNKEFDLAKDMNLFVF
                     ISSIQNEAHRFAVEFNRKKRNKRVYESELDNIPGVGENVKATLLRHFGSIRQIKAAGE
                     KQIADVKGVGAKRAKAIYNYFHNTQGG"
     gene            4461..5108
                     /locus_tag="PLQ68_01825"
     CDS             4461..5108
                     /locus_tag="PLQ68_01825"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HXK71416.1"
                     /translation="MAVYAIGDLHLSFQTNKPMDVFSSEWTNYEQTLMNNWQNIVTSQ
                     DTVIIPGDFSWAMYLEEAVEDFSYLSRLNGKKILLKGNHDYWWETKKKMTGFLQSNNF
                     SNIDFLYNNSFEIEDVNFCGTKGYDLKEADQKIINREISRFSISYQSIKNKGKKTIAV
                     FHYPPEKELEYMMKEYGIEQCVFGHLHGLKSESYQNEGHTLVSADYVRFTPVKII"
     gene            5221..6174
                     /locus_tag="PLQ68_01830"
     CDS             5221..6174
                     /locus_tag="PLQ68_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HXK71417.1"
                     /translation="MNISQVEKTALSIRDNINKVIIGKDEIIDLVLVSVLCNGHVLLE
                     DVPGLGKTMLAKSLAKSIDAKFARVQFTPDLLPSDLTGVSIYNQKEGEFVFKKGPLFT
                     NIVLADEINRATPRTQSALLECMEEHQISADGVTYKLDEPFFVIATQNPLETQGTFPL
                     PEAQMDRFFMRLSIGYPNEQEEIAILSRFEKDMPLTDLKSVASRDVILEASEAVNTIR
                     VPDDIKDYIVRLVAATRTSTKLILGLSPRGSLALMRAARAYAGIHGRDFVIPSDVKKV
                     APHVMGHRVMLKGHSLSYGAKGTMEVIDEILNTVPVPTEEL"
     gene            6182..7354
                     /locus_tag="PLQ68_01835"
     CDS             6182..7354
                     /locus_tag="PLQ68_01835"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HXK71418.1"
                     /translation="MELILIVISLVIFFTAEILFFKFFVLRNIEYSLQFSQSEAYEGD
                     DIFIDEVINNNKRVIVPWLKADIHASKWLQFANTSSSIIDEERCVSSGFSLNGRQRIT
                     RRWYTRCAKRGVFTIENTTLTAGDLVGIMQDSKAFVANTRIMVFPGVLKLEEAFFSNK
                     DLMGDMMIKRFILDDPFIVKGTREYVQGDPLNKLNWKATARMQKLMVKENDFTAKKGI
                     TVLFNIKSYALEYIDVKYKDVIELGIKVVNTIMERGYGMGFPLRFGTNAGIISNPEEM
                     IFSDSIAGPEHMRHLKVILAELILKNHLDFPEYIEKHCDEIFDTQVYIVSAYLNDEII
                     EKADILRRRGNTVKFLILNYTSDAVSDRGMEVFLLDRNTDFLKEGEYYGSGKTDKA"
     gene            7326..8735
                     /locus_tag="PLQ68_01840"
     CDS             7326..8735
                     /locus_tag="PLQ68_01840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK71419.1"
                     /translation="MDLEKQIKRKFLPLLGNIGSIGALAFSIYYAIFPESAVLWMVFC
                     FIVMLVGTLSGMIIVEQYEKKGKDIVRADYDNVRILLQPAYIFAVIPFVILLLVGLVF
                     TGQFLGSFLGYAFLPTVFFILGIGNGIFDYIQTAHRKMLYVALIPLAISLFVAFISSP
                     FGFYQDPVWLFGSIYLLFFLLVINRIKLEDMFVKSKKVNVENKKTIRRRNDFFILIFF
                     VLYLIIFVIRNTFTKMSDWIIRTAFAVINWIINQFYKLYKEIDVEEVTQVAEDVIEDG
                     PPAVTPPLNPVARTIFIVIASLVIAIVIFFLVKKIIQLIRWITGKVSDTVSTGVRKKK
                     DVENEEFEEIVEDLDKKKEKSKFRRAPKFKYTLSGLKQLDTYKEKVRYLYGFALERLI
                     IKKIEISKADTPEQILEKLKQQEHGDEFANDMFDEFTDEYINVRYGDKVKKQEDSLDD
                     KVDMLDRGINKIRPNKKEN"
     gene            8775..11228
                     /gene="lon"
                     /locus_tag="PLQ68_01845"
     CDS             8775..11228
                     /gene="lon"
                     /locus_tag="PLQ68_01845"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004622882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HXK71420.1"
                     /translation="MCTKIGRDDITTLKLKEIDMEDIKKDIEEIIDLNNDDDKELTGK
                     IEYMKIPLIALRAVAVIPSMVTHFDVGREKSILALEKAMEENGQVFLIMQKDASVENP
                     KKEDLYDYGTIANVKQVLRLPGKTCRILVEGVKRAKLEEVLSIKGYHTAVVSYVDMPK
                     AIISKSNALEAMHRKLTTLYEEYVTLLGRISHEGYFSAVAADDIGTVADAMVGSMALK
                     SEVIQSFIEELDGIERANKLLPLLVNEIEILKLERDLSTKVKSTIDERQKEYYLREQL
                     KAIQEELGEYNESLDEDEMSEYIKALESDRYPQEVKAKLTKEMNRLSKMSPMSPEASV
                     IRSYMETLVELPFGIETKERLDIKRVRKVLERDHYGLNKVKERIVEYIAAKKLSNDFN
                     SPILCLVGPPGTGKTSIVRSIAESINRKYVRMSLGGIKDEAEIRGHRRTYIGSMPGRV
                     AKAIKQAGSMNPLILFDEIDKLGNDFRGDPASAMLEVLDSEQNNSFRDHYLEISLDLS
                     HVMFITTANTVETIPRPLLDRMEVIEVTGYTEEEKMHIANKYIIPKQLKKHGMTKKQL
                     SITKAVLERVINHYTRESGVRNLEREIASLCRKAATKMQLDEIDEIKVTVDNLEEFLG
                     PKKYLYDTTFDQDEIGMARGLAWTQVGGDTLTIEVNIMNGTGHVELTGRLGEVMKESA
                     KAAIGYIRSQTDKLGINPDFYKDKDIHVHVPEGAVPKDGPSAGITLATAIISALSGKK
                     VKRDVAMTGEITLRGRVLPIGGLKEKLSAARRANVKQVLIPVENVRDLVDVPQQILDE
                     MQITPVKNMDDVLKNALSE"
     gene            complement(11275..12633)
                     /locus_tag="PLQ68_01850"
     CDS             complement(11275..12633)
                     /locus_tag="PLQ68_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015514945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HXK71421.1"
                     /translation="MGFENRLTEGSVIKQLIKFSLPLLLSNIVQALYSVADMLIVGWF
                     SGTYTMSAVNIGGQITMVVTNFVIGLAIGGTVLIAQYIGAKKNEEVDKTIGTLFTLMG
                     ILAVFFSIFMTFFSDVMLKLLNTPEESYDMAKNYVLICMWGIIFVFGYNAISAILRGM
                     GDSKRPLAFVGIASTINIILDIIFVGPLNMNATGAAIATIIAQATAMFLAIIYLKRSR
                     FVFNFHHTSFRIHKDKLKLLLKVGIPSSAQNVIVGISFLVLTSIINDLGGVTASAAVG
                     IVGKFNSFAILPAIAMSASVSAMSAQNIGAGLYDRAQKTLWSAITISFAISVAIFAFV
                     QIFPEAILQAFKAEPDVIDIGVSYLRSFSYDYLIVPFAFCFNGLLNGSGYSIVSMTSS
                     IVSSLVFRIPLVFLFARVLETGVGSLGYAAPIATVFASLIGGYFYFSGKWKKDKLKIK
                     RS"
     gene            12776..13924
                     /locus_tag="PLQ68_01855"
     CDS             12776..13924
                     /locus_tag="PLQ68_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007290370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine biosynthesis protein"
                     /protein_id="HXK71422.1"
                     /translation="MKSIAQINEKIKKGKAVVVTAEEVIDIVKEKGYKQAYEYVDVVT
                     TGTFGPMCSSGVFINFGHSNPPIRMQSIMLNDVPAYGGLAAVDTYIGATEVSTKRGME
                     YGGAHVIEDLIAGKDIKLHALSNGTDCYPNKEVLTTINKDNINQAYMFNPRNAYQNYP
                     AATNSTNKTIYTYMGTLLPNYGNITYSTSGELSPLINDPYLRTIGLGTRIFLGGGTGY
                     VSWQGTQHKVDVERSENGVPLVHSGTLAVIGDIKQMSTKFIRAAVFDKYGVTLFVGIG
                     IPIPIIDEEMLKFTSVKNSEIYTKLVDKSGNVSIDKKFNYAELRSGTVKFNGKNVHTA
                     PLSSIYIARNIATTLKEWIINKSFELTQPVAPLPFEPKVNSLDIREIK"
     gene            13924..14328
                     /locus_tag="PLQ68_01860"
     CDS             13924..14328
                     /locus_tag="PLQ68_01860"
                     /inference="COORDINATES: protein motif:HMM:NF020940.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HXK71423.1"
                     /translation="MSKLKLKLTFDKDCVTRPIIYDLVKKYDVMTNILNANIEYGNRG
                     NMILEIKGKEDNLKLALEYFDSLKVKYEIYAEAIVWDEARCINCGSCTAVCPTQALYM
                     GKDDLLHFDKDKCVVCQLCINACPLKIISVEL"
     gene            14337..15056
                     /locus_tag="PLQ68_01865"
     CDS             14337..15056
                     /locus_tag="PLQ68_01865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0280 family protein"
                     /protein_id="HXK71424.1"
                     /translation="MYQKRDYRKSFSNELKWYNVCVEETDLMVGTDENHSKQILKMIR
                     TLRHDIYAAIEKNQDFFTSLVPIDYPGAEGVVKMMVDAAKLANVGPFAAVAGAINDSL
                     ANELSKISKQVFIENGGDLFIISEKERKIGIYAGNSVLSNRFAVEIPKEAFPVGICTS
                     SGTVGHSFSMGKADAAVVIAKTSAIADAFATALCNRIKKETDIEPALEWVNSFEQVTA
                     AVAILEDKIGAIGNIKLVKWN"
     gene            15058..15861
                     /gene="proB"
                     /locus_tag="PLQ68_01870"
     CDS             15058..15861
                     /gene="proB"
                     /locus_tag="PLQ68_01870"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003472147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HXK71425.1"
                     /translation="MRTKLKNVQTIVIKVGTNTLTHSNGKMNLKSMEKLVREISELSN
                     EGKKVILVTSGAVGVGLGKLNLAKKPKTLAARQVLASVGQCELMHIYSKLFSEYGITV
                     GQILLTRDVTENPAKAENAYNTFKGLLDMGIVPIVNENDAISTDQLAIVDTFGDNDTL
                     SAMVAKLVEADLLIILSNIDGLYDKDPNQNQDAHIIETVTDVDDEIQSYASDLISSLG
                     KGGMKTKLSAAKTCMENNIIMAIINGQNMKNISRLIDGENIGTLFMKKK"
     gene            15875..17119
                     /locus_tag="PLQ68_01875"
     CDS             15875..17119
                     /locus_tag="PLQ68_01875"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HXK71426.1"
                     /translation="MLYIDELGLKALKAKDELVSINSKMKDEALRVMAQQLVKNSSTI
                     LSANKLDVICARERKVKESLVDRLLLTTSRIEGMAKGLIEASQYPDPIGKVLYETDMP
                     QGMKLKKITVPMGVIGIIYESRPNVTADAAGLCFKAGSAVILRGGSDAINSNKAIVRV
                     LRNALDMLGINNDSIQLVEDTSRETATEMMRAVKYIDLLVPRGGAALIASVIENATVP
                     VIETGTGNCHVYIDKECDEKKAVDITINAKCSRPGVCNAIETLLVHKDVYKRVLPKIE
                     EKLLEYNVQIRACERAKQVLNSSVDATEEDWATEFLDFILAVKVVDDIDEAIRHINKY
                     GTKHSECIITEIEENKKLFTNKIDAACVYVNASTRFTDGGEFGLGAEIGISTQKLHAR
                     GPMGANEITTYKYIIDGQGQIR"
     gene            complement(17120..18028)
                     /locus_tag="PLQ68_01880"
     CDS             complement(17120..18028)
                     /locus_tag="PLQ68_01880"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXK71427.1"
                     /translation="MEFRTLKIEELQQWTQFCGSVFPVGEAYFMRHYSNDPYRDINGI
                     FIAVDETGIASSVRVFTRYLKIKDTVIKVGGIGEVCTKKSHRGMGLSSKLMHMAIEYM
                     DKHDMPLSMLHTGTNHHYVKAGWFSICSYYKVYSLRKTKLQEGYSVEKADLKSMDVIK
                     TLFEEHAHKLCPVYVRTDEYYEKWIRSELTNTYYLLKDGKPVSYADCVLKANQGVLVK
                     EYIAGDETSMMKPFFYQICNQMNWPKMIAISSPFATDKSYFKFFDYGQMFRINKPFKI
                     GDKTISYEEDLLVYLSEFPELHLDGY"
     gene            complement(18054..>20327)
                     /locus_tag="PLQ68_01885"
     CDS             complement(18054..>20327)
                     /locus_tag="PLQ68_01885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK71428.1"
                     /translation="PTPSQTQAPTPTPTPSQTQAPTPTPTPSQTQAPTPTPTPSQTQA
                     PTPSPTPTPTPDSFGISEAYPTGTKEITVVFSAAAPSGATVLLNLGTQQVTGTSLSWG
                     TGRLSVKITKSTAFTPGSYSVTVSNITKAFTVEAEKLSEIVISAERIFALNNQDLKVI
                     LLNQYDNQLPLTAYTNFTFNASCQAKPSVLLSISKTSAGFILTGAQNLAANDTVRLLI
                     VENSTGTSVTKDITLSASPAIGSFSFGQIEYASGSTSILVNSSGHYITVNAKDQYGQN
                     YTITPSDVGLNKPVILASTDNLIIDATKITVTNGKLYLPSGAVGSKEGTVTLNAVGQS
                     ISSSIQINVTSPPVIKTLEIAGTDGNLYINQWVPLNATAKDQNGNIMQMDTLDGSKLM
                     MISTNATTIPNSNIRFNSTTKRIEIKGSSEGTATIHISYNGVSQGSLDISVLATPVPT
                     YIIGVDIPQIYQKGSTVLFNSSVLSIADQYENDIALTASNSVVVTCVGPSGAKFTEGT
                     VTGGGYTMTAPSTAGQYALTLTLKNGSAVISTYDLVLLVVEDEKIVSYQIQSIPTMYG
                     GATDTITDNGYWKPIIVTGKLASGQLVNLAYTGTMPIGIDQITCTNVDGANNFVIEDV
                     SGVKTLKAQNVTGTITTTVKLWKAGQELTSIDVTASGTAPTLTTLKSTSANISKAVSS
                     GTFQIGTYIEAKDQYGNIMALSSLQGSITYMSSKTTVAFINSSGIVSPVGAGTCNITI
                     YFAGYDKTLIIGLTITN"
     assembly_gap    20328..20427
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(20629..21762)
                     /locus_tag="PLQ68_01890"
     CDS             complement(20629..21762)
                     /locus_tag="PLQ68_01890"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HXK71429.1"
                     /translation="MNSKERLLTVLRGELPDHVPVAPDISQMIPIRRTGLPYWDIYLY
                     QRIPKWKAYIDSVKYFGFDSLMDGFANIQFEELGEFDDTWTEAIVHKDDLKIVTQKYR
                     TENNQKVWEPLVSVYHKNNPPSWDVHPHLVKLPLIPDKYEPVEDVSKWPTGEALLKWV
                     KEEMGDHGLVGITCGTSIILKNSEMVYDYFDNPNKYYEKRDQLMEYYEKRLHKLMSLE
                     HKPDFIGTGGSGSLIFQTPEIFRELAFPIVKRMAELCKQYGIPSHVHSCGPETALVKM
                     LAEETELTVIDPLEIPPMGDCNLKELKKLYGDKIVLKGNLHTTDVMLRGTVQDVIDAS
                     KRAIDDGAEGGKFILSTGDQCGRDTPDDNLFAMIETAKTYGRY"
     gene            21970..23232
                     /locus_tag="PLQ68_01895"
     CDS             21970..23232
                     /locus_tag="PLQ68_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HXK71430.1"
                     /translation="MIKAPKKNVNLAFVGLSKRGNGLLNMVLDSMDDVNITAVCDVYE
                     DRVNKAALDVLNKRKAEPLKTLDYKELLHRKDIDAVVIPTSWETHFDICIDFMEAGKY
                     AASEVGGANSIDQCWQLVKTYEKTKTPVMILENCCYGKTEMTVLNMVKKGIFGELIHA
                     QGGYEHDLRSEVAMGNHNRHYRLNNYKNRNGDLYPTHELGPISKWLDINRGNRMLYLC
                     SMSSKSAGINEWIRSRKGNDFENADFNFALGDIVTTMIKCAHGETILLIHDTSLPRPY
                     SRGNRLQGTKAIWLEDKMSFFIDGVSPASGWDHEWESFDKYYEQYEHPLWQWYRKKGL
                     VGGHDGMDYLSMRAFIESVKNGTDTPIDAYDMAAWMSITCLSEQSVAMGGHPVAIPDF
                     TNGRWINKRPVVQSKYCLDEVCNDEVYE"
     gene            23225..24088
                     /locus_tag="PLQ68_01900"
     CDS             23225..24088
                     /locus_tag="PLQ68_01900"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HXK71431.1"
                     /translation="MSKNIETSIKDWKIGVTSSGINHLSIEKYLEYKKAGIDAIELSY
                     NFEKDESFDIYKQKKLAKEAEIILWSFHLPWHHQLCNIAHLDKDMRNIAIDMDIQMIK
                     KSADCGIKYGVIHPSGEPILQEDRNEAMKNAKESLYKLSKTADKEGFTIALENLPRTC
                     LGNCSLDMNQLLEASDSLKICFDVNHLLQESHAEFINKIKGKIVTLHISDYDFVNERH
                     WMPGEGKINWPELIELLEGIGYEGIFMYELGLKSEKTIIRRDLNYSDFKNNFTALSEK
                     RKPDAIGKPNV"
     gene            complement(24093..24602)
                     /locus_tag="PLQ68_01905"
     CDS             complement(24093..24602)
                     /locus_tag="PLQ68_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXK71432.1"
                     /translation="MYNIEKTKQSDLIYLMEIFHQAQAALKKMNVDQWQNGYPSEQII
                     LSDIDEEISYVVKNNNQIIATAAIYIGNEPTYDKIYDGVWITDNKPYCVIHRIAVRDD
                     YKGKNIASYLINYGKQMAQLNNAESIRIDTHRDNIPMQKMLNKNGFIYCGIIYLLDGK
                     ERFAFEKPL"
     gene            complement(24614..25132)
                     /locus_tag="PLQ68_01910"
     CDS             complement(24614..25132)
                     /locus_tag="PLQ68_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017472916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HXK71433.1"
                     /translation="MEIWDLYDKNRNYLGKSKKRGDVFMNGDYHIVTHICIFNSDNKM
                     LIQLRQPYKKGWSGLWDITVGGSALHGEDSQACAEREVFEEIGYKLDLTEQRPYLTIH
                     FTYGFDDYYLINDDFDVNKLVLQKEEVAAVKWADLNEILNLIDNDKFVPYHKSLVNML
                     FDLHKTRGAHFK"
     gene            complement(25152..26273)
                     /locus_tag="PLQ68_01915"
     CDS             complement(25152..26273)
                     /locus_tag="PLQ68_01915"
                     /inference="COORDINATES: protein motif:HMM:NF024136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3810 domain-containing protein"
                     /protein_id="HXK71434.1"
                     /translation="MRKKIKSRKTNKTDTKKALKMLVASLCFGLFTIVIILIAKNSPK
                     FTNGIYSKWSVWMMDTMSKAFSFVKFSVAEILIYLLAIILTVSLIILIKNLIKNRGRL
                     GRLFKFITAWLLVLSIGIFLFYSMWGCMYYSDGLTGELSLEVKDREVEELIALNKYLI
                     EKANQLSTEVKRNEQGYYTGNEFNEMARLSAKAVSDFTNKQEALPKYVIASNAWSYTQ
                     TTGIFTFLTGEANINSNNLAIAVPFTCAHELAHRNGITSEDEANFFAFYTLIKSDNIN
                     MRYSAYSMALIFSMNSLYSKDAQSHRQLSELYSDHLRNDYDRYSEHWDRYDGKVAEIS
                     DKANDAYLKAQGETDGVQSYGKVTDILLAWYEKDIDSSN"
     gene            complement(26239..26415)
                     /locus_tag="PLQ68_01920"
     CDS             complement(26239..26415)
                     /locus_tag="PLQ68_01920"
                     /inference="COORDINATES: protein motif:HMM:NF024081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HXK71435.1"
                     /translation="MKLIDYVIIAIVAAIVILVIWYLVKNRKKGCSSCGSCSACPYSG
                     QCEDEKKDKKQENK"
     gene            complement(26424..28421)
                     /gene="feoB"
                     /locus_tag="PLQ68_01925"
     CDS             complement(26424..28421)
                     /gene="feoB"
                     /locus_tag="PLQ68_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004617111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HXK71436.1"
                     /translation="MIIALAGNQNSGKTTLFNQLTGSNQHVGNFPGVTVDVKEGISKS
                     DKSVKLVDLPGIYSLSPYSGEEILARDFLLKEHPDGIINIIDGTSLERGLYLSMQLLQ
                     LNIPMVIAINMMDEVFANGIVIKTNILSDYLKVPIIPISANKNEGIDDLMKAMIDLIK
                     NKCAPPRQDFCSGPVHRTLHATAHILEDHAQRASVPASFAAVKIIEGDEPMLNDLSLG
                     KNEKDMIEHGIVEMENELSMDRMAAIADMRYQYIEEITQKAVVRPKTDKHHDKTIKID
                     NILTHKIFGIPIFLSIMFVIFWLTFAVVGSFLSDLLSGGIDSLASVVSKGLNNFGINP
                     VVESLVVDGVFAGVGSVLSFVPTIIVLFFLLSLLEDSGYMARVAFIMDKLLRKIGLSG
                     KSIVPMLIGFGCTVPAIMATRTLTSDRDKKMTVFLTPFMSCSAKLPIYSVFAMAFFTK
                     YRAVVMIAMYVIGILLAIVAALISRKFVFKGNPIPFVMELPNYRLPTAKNTMLLLWEK
                     TKDFITRAFTIIFIASIVIWFLNKFDIRFNAVENSKDSILALIGSFIAPIFIPLGFGN
                     WMASTALITGFMAKEAVVSTFAVLLSTQTESISSQLGTIFDPISAFAFLLFTLLYTPC
                     VAAIAAVKRELGTKSMFVVVLLQTSLAYVIALIFYRIAILF"
     gene            complement(28418..28648)
                     /locus_tag="PLQ68_01930"
     CDS             complement(28418..28648)
                     /locus_tag="PLQ68_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA domain-containing protein"
                     /protein_id="HXK71437.1"
                     /translation="MTLDKLAIGKYGRIIAVRGRGRLRHRLLDMGLTPRVIVHMQRKA
                     PMGDPISIYLRGYELTLRLEDASLIEVEEVSV"
     gene            28887..30722
                     /locus_tag="PLQ68_01935"
     CDS             28887..30722
                     /locus_tag="PLQ68_01935"
                     /inference="COORDINATES: protein motif:HMM:NF019552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HXK71438.1"
                     /translation="MKLFEGFTCKGKLLSNDDFLKRLRNDTEKGIDKLSAEEKQHYID
                     NAEKAIETEIPILKASVYKQFAYTGNRTEYQGPYYSRRNMLTELIFAELLEKKKRFTE
                     KILDLVWLILEESTWVIPAHNKNGSSLLYEFDDVSYVDLFSASTAAVLALSYVFFKED
                     MNDGLTDDMFSKRLEYEIENRILLPFEKYAKNWWYIGHIPGHKLNNWNPWIHSNILFI
                     LAIFEKNEQRKEKLCDDMLSYTDNFLDTYGPDGGCDEGPSYWGAAGASYFDFLDLLYK
                     ASDGKIDKLSEKLIYNMGDYVRKMYIGGGYFVNFADSGPKPKNLDAVLIYRFAKRTGN
                     KLMMDFACSFDQCNRISYSHPQRSLYNMIEKSECKQSSYKPNKEEIMEDTQVYVFRND
                     THFIAIKGGHNREAHNHNDIGHFVLFEEDTPILIDAGSDTYSKKTFSDERYTLWNMQS
                     SFHNLPEISGYMQLFGIEYKASDVSYDKNSKCLSMQLKNAYPKEANINSYIRKLYVRN
                     SELIIEDDIDLKEEGDITEHLLLLYEPSIRDNFITVKNTQIAIDNVNDITYEKISTLN
                     YTGLNTNVPERGNMAKSWDSDFMFRIKASAKTKNATIRFTINRRD"
     gene            30723..32999
                     /locus_tag="PLQ68_01940"
     CDS             30723..32999
                     /locus_tag="PLQ68_01940"
                     /inference="COORDINATES: protein motif:HMM:NF028476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK71439.1"
                     /translation="MAYEWYGHFSNEGKNYVITQPNTPRHWYNYMYNDEYITFSSQVG
                     FGEGFAQDNMGRRVPLVMNRNFFLCEDEYWSLAGLPINYGYTDYSCTHTAGSTTINLR
                     YNDISSSVRIFVPNNKMQEIWTISLKNHSNRRREISLIPYAKTDVDSPYKPQGYNIAT
                     GDVYKEKNCVYGFFYQAFNSQRNLPMYGYMSSSQKISGFDTRKTAFVGAYSDEQRPEA
                     INNNLKCTNSSCFAEKLCFALQNDIVLEANEQKTLHFVLGVSFSKEEIVTPEDKWVQQ
                     QFQAMEDKYEQRIRQVQIKTPWENFDNLVNGWLPYATDMGSRWARVRHNGYRDMSQDT
                     DCFSVINPSLAWERIKRVLTYQYDNGYAPRTIIDGVLKDRNFSDNTVWLTFAVYDIVK
                     ELGDITILNEQVKFNNSKQASVYEHIRRSVDFLWNFTGLYGLIRIWGGDWNDCINYAG
                     LEGKGVSVWLSIAWCLAAKQIAELSGMLGFDDYEKLYQERYQIMKDRIDQHGWDGQYY
                     IYARTDEDIVLGSHTQEEGKIFLNPQIWAVFANLNKDNRHIKSMDKAEELLQSDEGIL
                     LSKPPYTKQYDYIGSMAEKPGGIQDNGGVYLHPAAWKLAADSILKNNGRVEENLKKML
                     PFDNDYYETKGEPYCMFNCYYLDYHGYRRGTPGQSWRTGTNAWLLKSTVMYIFGLHPE
                     MEGLRLEPCLPESWRECSITKKFRNCKYIVKYIQTEDGACNNISKILVDEEEKDFDNF
                     IIPPVEGKQLNITVLLTK"
     gene            complement(33056..33706)
                     /locus_tag="PLQ68_01945"
     CDS             complement(33056..33706)
                     /locus_tag="PLQ68_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018592195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HXK71440.1"
                     /translation="MDIKALIFDLDGTLLNTLEDLTDSMNYALSSFGYEEISIQQARS
                     YVGNGAERYLELSLINGRENPDFQKCLDTFKTHYQANMENKTKPYDGILDLLNKVKVK
                     GYQTAIVSNKFDDAVKQISPKYFNSYIDVAIGESEKIKKKPAPDSVYLALEKLGCDKS
                     QALYIGDSDVDAKTASNSGLKFIGVSWGFRDKSILYELGAQTVIDHPSQLIKILEK"
     gene            complement(33717..34457)
                     /locus_tag="PLQ68_01950"
     CDS             complement(33717..34457)
                     /locus_tag="PLQ68_01950"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HXK71441.1"
                     /translation="MSKYKCLIFDIDNTILNYDACAASSLKALLEKYDIQYTNEIENL
                     FRDICYKLWKAFDLNMLNDEGIRKNYHQLFDLYNYKRFEVFCEHMKYSSLNDTNPKIL
                     SDEYIDIFNTTRIYENCAVEILKKTSKTHILAAATNGLSDSQRKRIQPVEQYLSHLFI
                     SEEMNCVKPMKEFYDYMLHKMNLNNTDCLFIGDLYTTDIVGALNANMDACWYRANGRN
                     SENWLEASTNYDENIPVIDSLDKILDYI"
     gene            complement(34450..36597)
                     /locus_tag="PLQ68_01955"
     CDS             complement(34450..36597)
                     /locus_tag="PLQ68_01955"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HXK71442.1"
                     /translation="MDRFNLERRIKAEKWADSILAVMTLDEKLHQMAASFPNGNARLS
                     IPHMQQGECLHGAVTPYATSFPQALALGSTWDVQLIEQVATVIAKECRACGIHQCYGP
                     MVAVVRDPRWGRIQEAYGEDAYHVSRIGLAFTLGLQGTSDKYLDKDHIIATVKHFVAD
                     GEPLAGLNGAAMEISERSLREVHLLPFETIVKEGHVCSVMPAHHSLNGVPCHCNKYLL
                     QEILRDEYKFDGIVVSDNGDIRKLHTALYVSNDSEQSAVMAVNAGVDTELAWMIPWNE
                     NRLYGPVLKNAVENGKIDIEKINKAVKRVLIKKYELGLLDDPTADPEQESLEKCYSTN
                     SGQEHVSMAGKTLYFGKPRKDRDTVCYNKEHDALALKAALKAVIMLKNENSVLPLNKN
                     SINKMAVIGPNADRLLLGNYSTDKPKYYISVLDGIKKYAKGNFEVSYSQALDQNDYSA
                     IDIPDAVRKAKSSDVTILVLGGNEITCMENEDVDDLALKKDQQMLFEKVRKVSKKLIL
                     VILDGRPSSFEWAKDNADAILLGFYLGQECGNAVALTVFGENNPSAKLPVTIPRNVGQ
                     IQCHYNRYWPGRAPSYYKSAVEPLYPFGFGLSYSSFDISAPIIEKERYTVNDDIPVSV
                     TVTNTSDVKGEETVQIYIRDLVASLVRPVIELKRFRKVELLPNETKTLCFTLRPRDFS
                     FWKDKKWTVEPGTFIIYAGNSSTNLKETELNLE"
     gene            36748..39009
                     /locus_tag="PLQ68_01960"
     CDS             36748..39009
                     /locus_tag="PLQ68_01960"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXK71443.1"
                     /translation="MTEEAGHILTNKPVPNDISDEIKKLTDNERLLFTIVGDLSKNSK
                     YSQSVLAVTDEAMICFDETYENKYLYLKHGDISDIYIKRLYGNAIMLVKTEEEYFEFF
                     RFTYSVANLCEAAADFSKKLKLKEDTMKAMEAVEAVFEKQFSTCPKCGRTLVRPGAEC
                     MNCQPKGKIAQKLMKYIAPQKKLLIICLIFSLVTTAMSLVPPYITKMMVDDIIPKNNK
                     SLLITVVFVLLGTYVIQYSVGALRSYLLRMSGDRIVAGLRSDIYAKAQYLPMKFYDKT
                     STGSVITRISSDTSTLQSFTLRITQEVIVQFFLLIGIIIIMFVMNWKLTLLSLMPVPL
                     VVVGSRIFGKKILPYYRRIWRKWSAVSSILTDTIPGVRVIKAFTNEKGAIDKFDRYNE
                     EWLKTDIKAARITTLFPQIVSFFVTCGSLLIWSVGGNLVMNSQQGLTLGLLVSFISYT
                     SMFYGPVNFFANFNDSLQSAIAAAERVFDIIDAEPEPDFGKGSHPKKLNGKIQFKNVN
                     FSFDRTKMTLKNINVDIEPGDIVGIVGTTGSGKSTLINLLMRYYDNYEGQILIDGKDI
                     KEVDMEFYRSQMGYVQQEPMMFHDTIYNNIAYGLGNVQVEQVINAAKIANAHNFIIKL
                     PDAYDTVLGERGTGLSGGERQRVSIARAVLRNPSIMIFDEATAAVDSETEMLIQEAIE
                     RLIAGRTTLMIAHRLSTLRKANKILVVDEGQIIECGSPDELLAQKGKYYKLIEIQTMS
                     SKLQKAKEEEKLE"
     gene            39019..39507
                     /locus_tag="PLQ68_01965"
     CDS             39019..39507
                     /locus_tag="PLQ68_01965"
                     /inference="COORDINATES: protein motif:HMM:NF020483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1854 domain-containing protein"
                     /protein_id="HXK71444.1"
                     /translation="MRSPYIEGDNAKITRKDLTHVNVLMYNGQEILDVEPRRLFPISG
                     LRKYITLLDKDEKEVAIIRNLDSLMEESKTAVEQCLDEYYLIPKIQKIYEIKEISRNM
                     NMYVLTDKGERRFEIANRHNDIKVIHGTRVLFRDISDNRYEIEDADKLDKSSKIRLGA
                     YI"
     gene            39532..39858
                     /locus_tag="PLQ68_01970"
     CDS             39532..39858
                     /locus_tag="PLQ68_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002606956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HXK71445.1"
                     /translation="MKLILAIVNNDDSAIAASALTEAGYFVTKLSTTGGFLMVGNTTL
                     LIGTEDTETENVIEILSKYCKTRKQATPSTASFGNGLSNESLPPEVTVGGAAIFVMNV
                     EKDIKI"
     gene            complement(39943..40392)
                     /locus_tag="PLQ68_01975"
     CDS             complement(39943..40392)
                     /locus_tag="PLQ68_01975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK71446.1"
                     /translation="MKRKYEKMSDLQKANRLKNHYIIHIALYAFMFNLLPAAFISANM
                     EGFNLYMLLLGYPAVTYIASIIFALYNRYKWLYILFPSVLFIPAAFIYYNYTALIYMV
                     LYAAAAALGLLVGIAFIWIFTKKDDMSVKKTKNMTTSSISKKAKKKG"
     gene            40511..41704
                     /locus_tag="PLQ68_01980"
     CDS             40511..41704
                     /locus_tag="PLQ68_01980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U34"
                     /protein_id="HXK71447.1"
                     /translation="MCDTLGCVNQNVPIFGKNSDRCVGEPQLLEFYPRRNNIEKTFMA
                     TYIEVEQAEQTNAILISRPSWMFGAEMGINEYGVVIGNEAVFTKGGYNKIGLTGMDLL
                     RIALERSADAKQALQLIITYLEKYGQGGNCACEGEKYYNNSFLIMDKKELYVLETYDK
                     QWAYRKAEKDSISNRLCIGTEGECSTGKECNFAKIHTEPVYTYLCGSGHRRNRTQTCL
                     EQKCDLNGFRKALRSHTKVKDPFNSGTANSVCMHAGGVVDNQTTASIIVQLYPTAVIW
                     ATGSSLPCVSLYKPYLFGTKAPFVFAPDDTSSVKYWKERETFNRRMLGNSPPKEYYDY
                     IYELENQWEESVKLNYADAKAMEEISNKAFEQEQQFFEKMDKIISKTPKPTFWHMGFW
                     NKREV"
     gene            complement(41705..42259)
                     /locus_tag="PLQ68_01985"
     CDS             complement(41705..42259)
                     /locus_tag="PLQ68_01985"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HXK71448.1"
                     /translation="MLDTIETDRLILREFTFDDVEDLYEYAKNPNVGPNAGWTPHKSL
                     CESEAVLNMFMRNDDVWAIVYRRNNKVIGSIGLHSDLLRSTVKSLKAKCLGYALSYNY
                     WGLGIATEAVKAVQRYAFEILELDLLSVDHYPDNKRSMRVIEKCGFKYEGTLRKARPS
                     YDNKPMDISCYSLLKDEWKKINNR"
     gene            complement(42271..44451)
                     /locus_tag="PLQ68_01990"
     CDS             complement(42271..44451)
                     /locus_tag="PLQ68_01990"
                     /inference="COORDINATES: protein motif:HMM:NF036578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HXK71449.1"
                     /translation="MYHKTIFKNAHWIMPSMDMDSAIFRKTFINKGCKKAVMTITGLG
                     YFLLYINGKKVSDDLFTPAYSDYHPRDTKKFRYPISDTMNHRIYCLKYDVTEYLKDGI
                     NCLGIMLGSGFYNQTKRIGEGNVTYGRIKLCFRLSLDDEYIESDKSVMYKEGFIKSSN
                     LFYGEEHNYNGFDYLWNTENADTSGWLDSIETDIPQSELYIQNCNKDKIIKKIKPKLI
                     DHKDGLFVYDAGINISGYPVIYCEKKNEHIQVECAERINDDNTLNFISMGGGKGQKCT
                     MKYITDGITNTFNPIFTWQGFRYFTVTDNATALECHIVHTDVKVNSSFSCSSSILNWY
                     YKAYIRSQLTNLHGSVPSDCPHRERLGYTGDGQLCARAAMYCLDMKKVYRKWLEDISD
                     CQDINNGHVQHTAPFNGGGGGPSGWGGAIVTVPYEYYLHYGDLSVLRKFFPKMLHYFE
                     YMESRCSNGLVVKEEEKGWCLGEWCTPEPTYIPESYVNTCLYVKLLQIGQTTADILKK
                     FEDKKRISKLEQIHKKAITDAYMSKLESTFIFGNQGADAFAYDIFLGDERTLKNIKRR
                     YESLLTYDTGIFGTYILNKVLFENGLSDLAFLLLANKKEASFYNMKKHGATTLWERWN
                     GDSNNHPMFGASARYLFEYVLGIQQKPDTSGFDEVVISPLKIEGLNRAKGHITCRHGK
                     IAVSYMKSDQITEYRIQIPKNVNAVFSVGTIKKILNEGLNIIKI"
     gene            44584..45120
                     /locus_tag="PLQ68_01995"
     CDS             44584..45120
                     /locus_tag="PLQ68_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lyxose/D-mannose family sugar isomerase"
                     /protein_id="HXK71450.1"
                     /translation="MKAKDVEKAKKEALLMYNKAHIVLTEKEKEDIEIADFGLDDFYK
                     TGLSIVTYVNTDNCCAKEMVLHPFQTCPEHIHAPIEEIKYKGKEETFRVRYGKVYLYV
                     EGETSKKVSVKIPDEYYTVFHEIELNPGEQYTLYPNTWHWFQAGEKGAVISEFSTSSH
                     DECDHFRNPNIERIPTVE"
     gene            complement(45121..46713)
                     /locus_tag="PLQ68_02000"
     CDS             complement(45121..46713)
                     /locus_tag="PLQ68_02000"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HXK71451.1"
                     /translation="MKTYLIQPAYPKTKSDKKETFNFIINSLDNITEDADLILLPEFS
                     NIPGASTLKEMIEDDKNNTSILIEKIISTAKRTEALIAVNIAYDTKTGYRNSTFLYNS
                     DGKVIFRYDKQHLTYAEKEILKLDYSYKTRPYIPCCIEVNGMKIAFMTCYDTYFLEYI
                     EYIASKKPDLILISSYQRSESDDNIFNQVKMISSRCNSYVLRSSYSMGDKLTGGHTLV
                     CDCSGKILNDIKQSVGILDNHVDISNKCKRSNGHGQGLIDSDEFITQGRTPYSYRPAG
                     SFISLNDNEKPYPRICAHRGFSALCPENSVLSLASAIALDADEVEFDLWPSEDYEIIA
                     THDPVFEKNSQRKVWEYAFDDIMKLDASYQMSSQLEGLRYNTFEEILRKFNHQTIMNI
                     HIKTKFINGVNIHPYDEEAFRIIYNLIKEYGCEEYVYIAGDEAVLETARKIAPHLSRC
                     CLAGQQDYSIVDKAIQFGCKKLQFYKPYFTQEMIDQAHANNIKCNIFWSDDPKEACEF
                     LDMGIDTILTNNLHLMKNGLLR"
     gene            complement(46710..47216)
                     /locus_tag="PLQ68_02005"
     CDS             complement(46710..47216)
                     /locus_tag="PLQ68_02005"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HXK71452.1"
                     /translation="MEYAIRTAEKKDVEDIVRLLKQIAQLHHIGRPDIFKSNANKYTH
                     DFVHFKIKNPEERIFVAVDNLDKVCGYIFCYFEIRKEHAVLKERKILCIDDICIDDNK
                     RGIGIGSLLINHAKNFAESAECSAIELNVWEFNEDAMKFYEKHNFKTKYRRMELLLNN
                     EENTKGKL"
     gene            47377..48195
                     /locus_tag="PLQ68_02010"
     CDS             47377..48195
                     /locus_tag="PLQ68_02010"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013788485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HXK71453.1"
                     /translation="MIISNKYFKDGKPKALTMSYDDGPYEDYRLVELFNKSGIKATFH
                     LNSRYLDRDDYPPQPEKIKMKDVKNLYEGHEISVHTVNHCDLRAVNNNRFMHEIVDDK
                     AALEKLAGYPVRGMSYPYGYYNDDAVSHLKYFGMEYSRTTKSHKSFYMPDDFLVWHPS
                     CHHIDAIELISSFDSPRWNMEMPLFYVWGHSYEFSRFGDKWYLIEDFCKRISGREDIW
                     YATNIEIKDYSDAVKNLKFSADDSMVLNNSCIDVWIDVDRQPVKIPKGELVKLK"
     gene            complement(48198..48866)
                     /locus_tag="PLQ68_02015"
     CDS             complement(48198..48866)
                     /locus_tag="PLQ68_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HXK71454.1"
                     /translation="MTTPLYEDLSKNVELFRNIEPDCLKSMLSCLGAKTVTYRKNEII
                     LMTDDSLESIGLVVQGAVLITTEDFLGTRSVIGVAEKYEIFAEAFVCANISKSPVTIS
                     ATANCVIMWLKFPRIIQSCNKACEFHSRLIENMMNQLASKNLQLHQKLEILSKRSLKE
                     KILAYLNIYATKTKNRNFNIPLSRNELADYLNSDRAALSRELSKMKEEGIIDYNLNTF
                     KIMY"
     gene            49006..49722
                     /locus_tag="PLQ68_02020"
     CDS             49006..49722
                     /locus_tag="PLQ68_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HXK71455.1"
                     /translation="MIRKIIKIDEEKCNGCGICAKACHEGAIGMVNKKAKLIRDDYCD
                     GLGDCLPACPTGAITFEQREAEPYNQAAVDAKKKREEKARAVPQASCSCMGSVAKTHD
                     RNVQETSSCESCTPLTSRLRQWPVQLKLVPVEASFFNNAHLLIAADCAAYAYANFHED
                     FIKNRITVIGCPKLDSVQYSEKLTEILKRNNIKSVTIVRMEVPCCGGLEYAAKEALKS
                     CDKMIPWQSVTISSDGRILD"
     gene            49829..50017
                     /locus_tag="PLQ68_02025"
     CDS             49829..50017
                     /locus_tag="PLQ68_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004614733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HXK71456.1"
                     /translation="MKVTIDQDGCIGCGLCADTCPSVFCMNDDGVAEVCGEVDETNED
                     LVREAAEGCPVSVIEVEE"
     gene            50030..51619
                     /gene="hcp"
                     /locus_tag="PLQ68_02030"
                     /gene_synonym="priS"
     CDS             50030..51619
                     /gene="hcp"
                     /locus_tag="PLQ68_02030"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HXK71457.1"
                     /translation="MSDMFCFQCEQTAKNFACEGKKGVCGKEASTSNLQDELTGALIG
                     LARAAVSNGSYISTDRVMLEGLFTTITNVNFDDESINKLIDKVHDEKNLLAPQCAFCS
                     SDCSKSEDYDVSNLWKDNEDIRSLKSLILLGLRGMAAYAYHAMVLGYTDDEVTEFIYK
                     AMFVLGEDWDMNDLLPIVMDTGKVNLKCMELLDKANTETYGTPVPIKVPMTIEKGPFI
                     VISGHDLKDLHILLKQTEGKGINIYTHGEMLPAHGYPKLKAFTHLKGNFGTAWQNQQK
                     EFKDIPAPVLFTTNCIMPVKDNYADRIFTTEVVQYPNMVHIGEDKDFTLVINKALELG
                     GYKEDHVIKGINGGSVLTTGFGHGTVLSVADKVVDAVKQNKISHFFLVGGCDGAKTGR
                     NYYTNFVKQAPKDSIILTLACGKYRFNDLDLGDIEGLPRIMDMGQCNDAYSAIKVAVA
                     LSEAFGCSVNELPLTLVLSWYEQKAVCILLTLLALGIKNIYLGPTLPSFISSNVLDYL
                     VKNFSVSPISTPEEDLKKILG"
     gene            complement(51777..52745)
                     /locus_tag="PLQ68_02035"
     CDS             complement(51777..52745)
                     /locus_tag="PLQ68_02035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK71458.1"
                     /translation="MKSKKILIIALLIAILVSLAGCQLAIEDMQEIATEDILIGALIT
                     LEPLILFDSDSYINENIDRIMSENGQDIDISKYEGRLYAKLVTKTHTNEETNQTSESS
                     EYVFEDIEGITYIAATISDSDGVYTLASSSDGISDGHTKVTGGNTQKEINLEGTIYVS
                     ITSTNRAFFISPVYQSSNGDVYAVTGSGYISGQTEVEGSVFSQTLEDSKTITENGITK
                     TQKSSVKISISYIHSPFKISIYQMDKNNSIVRHNNFVPDEVPDNISLDSDTDYIIVET
                     HKYNDENNLVISREIHDKDEDYINTFYCMENGICSKKYVKLIWQID"
     gene            complement(52735..52965)
                     /locus_tag="PLQ68_02040"
     CDS             complement(52735..52965)
                     /locus_tag="PLQ68_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005809987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HXK71459.1"
                     /translation="MAILVHLEDILKERGMTSKELCSLIGITEANLSILRSGKAKGIR
                     FNTVNKICHYLNCDVGDILKFDGNLEDDKDEE"
BASE COUNT        17742 a         8537 c         9979 g        16668 t
ORIGIN      
        1 atttattcca agctatatta acaatttctc aagtatattg tataatagca atggtatgtg
       61 ttaagggggc atcatgaatt actacgatag gatacaaaaa tccatagagt tcatagaaag
      121 atacatcgat gagtccattg atattcaaga tgctgctgac atttctaaaa tgagttacag
      181 tgagtattgc aggtttttcc atgccatcac aggatataat gttcaagaat atataggtaa
      241 aagaaaaact gcatatgctg ttaatgatat gttgaaaggt aaaaccgata taaactattt
      301 agctaagaaa tactgcttta gcagtaccga agcattctgt atgatattca gaagtaatac
      361 aggatatcta ccttcagagt ttcttgacca ggatatttac tattcatttg aaatgattga
      421 tgttcgcaaa atttattatg atactcagga taaaatgctt atagaacgtt ttccggatat
      481 ccggttgcta agacctttcc caaaaatgag aatggcttca tatacaagtt ttggtaatca
      541 gccggaaaac aaggcatgca aggttattat ggaatatgct aaagcaaata aatatctgga
      601 ttcatataaa gcttttagaa tatttggatt taatgtatct gactccataa aacatgacgg
      661 gacatatggt tatacagtat gtatgacagt tgatgattca catgagtttt cagacggtcc
      721 tattaaggag ttaataattg agaatgggca atatgttgtt gcaacaacga ctgtaaacaa
      781 tctagttgat acatgggaaa gatttaaaga atggttgaga gttagtggaa tgaacagtgg
      841 agaccaccag taccttgaag agcatctaaa ttgctgcaat tggagagttg aagagccagt
      901 gaatgatact gaaattaatt tatatatgcc agtaagcagg aatagcaata attcaaaaag
      961 ataaatatca ttaatattga atataaataa taaggatgca gaaaggtttg aacaggtata
     1021 cttgtttata tcttctctat agcataaaat agttgttagg agtgacgata aaaatgacta
     1081 aaacatatgt tattgccaca ggcaatgaac tagatactat agaacttgga aaggttatgg
     1141 cagctgtttt tgaaaacaga gtagttgctg tttcgggtga tttaggtgca ggaaagactc
     1201 atcttgcaaa aggattcgct ttaggcttag gcatagaaaa gcatattacc agtccgacat
     1261 ttaatattat gaatgtatat acaaagaatg ataagacatt ttaccatatt gatgcataca
     1321 ggcttaccga tccggatatg atatatgata taggtattga agaggctttg gagccaaaag
     1381 gcataacctt gatagaatgg gcagaaaatg ttgaacatat attgaataaa ggatatgtga
     1441 agatatatat tgaaaaaaca gatgacgaga ataaaagact tatgtttata acagcagata
     1501 aagaatctat agataggatg gcaaaaatta tttatgaaga tactgttagg tgatacttgc
     1561 ttaaaaactg cttcagcagc gatttctgaa aagaataaac ttatagcata tatgcattca
     1621 gaagggcata cccctcattc agtcatgttt ccaaaacttg ttgaacaggt aattaatact
     1681 gcacagttac aattttctga tttggatgca atagcatgtg tcaatggtcc tggttctttt
     1741 actggacaac gaataggtct tacttacatg aaaacattag catatacact agataagcct
     1801 ttttatacct taaatacgtt ggatgtgctg gctaacagtc agaaagccaa agatgatgtt
     1861 ttatcactac ccttgataga tgcaagaaac aacgaagtgt ttactgctat gtatatgaac
     1921 aaagatggtt tttatataaa cattacgcca tattccgctt tgcatattga taaagttgta
     1981 gaaatgactg taaaaatgtg tgaaaagaac gataatcttg tggttatgtt gtcaggggat
     2041 gctcagaaaa agcatatagg tatttttaag aatgctttag ataaaaaaat tatggattat
     2101 tctgcagagc gtgacaatgt ggatttaacg agggctcctt tacttttaac agaagaaaat
     2161 agggaagatc cttttttggc tcaacctttt tatcttagag aatcgggagc tgtaaggatg
     2221 aagaaaatca aagaatgaaa gtaacagtca gaaaagccgt atgtgaagac atcgacggaa
     2281 tatttaaaat tgaaaaaagc tcttttacaa ataactggtc atatgagagc ctttatgaag
     2341 acatatgcaa aacccctatt tcgtattatc ttgttgcaga gacagaaggt aatatattag
     2401 ggtttatagg tatgtggcat gtaatggatg aggcacatat aatgaatgtt gcggtagatt
     2461 ccgggtttag gcatttaggt ataggctcaa tgcttctatc aacattaatt gaatatgcag
     2521 aagactgtgg tatattaaga atgacacttg aagtaagaga acataattac aatgcaataa
     2581 gcctttatga gaaatatgga tttttaaaat atggtaagcg aaaacagtat tatcaggata
     2641 catttgagga tgccatcata atgtggaagg aactgtagat gcccaggttc aatgaaaaaa
     2701 tagaatatct gcttaataat ctgcctgaaa aaccaggtat ctatcttatg aaaaatgctg
     2761 aaggagagac tatttatgta ggcaaagcag tcaatctgaa aaacagagtc agttcatact
     2821 ttacaaatct ttcaggacat tctgctaaga cccgtaagat ggttaacagc attgactcat
     2881 ttgaatacat ggttttggat aatgagagac aggcatttat aacagaaagc gatttaatta
     2941 agtcattacg accgaagttc aatattctta tgaaggatga caagtcttat ccatatataa
     3001 aagttacggt aaatgagaaa tttcccggta tatatgtaac tagagataaa actgataagt
     3061 ttgcaagata cttcggacca tatcctagca aggtatcaaa gaaaaatacc attacgcttt
     3121 tacaaaatgc ttttccattg cgatcctgta aaaagatggc aaagagacca tgtcttaatt
     3181 atgaaatgaa tctttgcctg gcaccatgca gcttagaatg cgatgtggat aaatataatc
     3241 agacagtgac ttcagtaatc agttttcttt ctggtgaaag tcagaaggta atcgacgatt
     3301 acaaatataa aatgcagaat gcaagtgata atcttgattt tgagaaagcc gcatattttc
     3361 gcgatagaat caatgctctt accatgatat ccgacaatta tgctctggct acagaccaga
     3421 attctgtgga tgttatagca tatgctgaaa aaaaggatta catttctttt tttataatga
     3481 atataagaaa aggaaaaatg tctgatacta gagttataaa cataaaacag gagagaattg
     3541 aagatactga tataggatat gaattcttac gtcagttcta tgataacaat ataccgaaaa
     3601 atataataat gtataatgag atatctaata gagaagatat tgaagactgg ttatcggaaa
     3661 atgcaaaaag caaagtaaaa attacagttc ctaaaaaagg aaaattcaaa aaactagctg
     3721 ataccgcata tgaaaatgca ttagtggctc tgaatctata tatgggtcaa atcaaaaata
     3781 tggcagatgt aagtccagta tactcattgg catcgttttt acagttagag actgttcctg
     3841 aaataattga agcatttgat atatctcatc tttcaggtca ggatgcggtt gcatcaaagg
     3901 tggtatttaa agaaggaatg ccatataaaa agggatacag aagatatatc ataacaaata
     3961 gtaattcacg gtcagatact gattcgttaa aggaagtttt ggaaagaaga atgaaaaagg
     4021 gtgatttacc tgacctattt ttaattgacg gagggaaaaa tcaggtatca gctgtaaagc
     4081 agatagtaaa caacgaggtt gaagttgcgg ggatggtgaa agatgacagt cacaagacaa
     4141 aggctttaat ttacaataac aaggaatttg atcttgcaaa agatatgaac ctttttgttt
     4201 ttatatcttc catacagaat gaagcccacc ggtttgctgt tgaatttaac agaaaaaaga
     4261 gaaataaaag agtatatgag tcagaacttg acaatattcc gggagtaggg gagaatgtaa
     4321 aagctactct tttaaggcat ttcggttcaa taagacagat taaagcagct ggtgaaaaac
     4381 agatagctga cgttaagggt gttggtgcta aaagagcaaa agcgatatat aactattttc
     4441 ataatacgca aggaggataa atggctgttt atgcaatagg tgatcttcat ctgtcatttc
     4501 aaacgaacaa acccatggat gttttttcat ctgaatggac gaattatgag caaaccttga
     4561 tgaataactg gcagaatatt gttacttctc aagatacagt gataatacca ggtgatttca
     4621 gttgggctat gtacttagaa gaagcagttg aagattttag ctatctaagt aggttaaatg
     4681 gaaaaaagat acttctaaaa ggaaatcatg actactggtg ggaaacaaag aagaaaatga
     4741 cagggtttct gcaaagtaat aatttttcca atattgattt tttatataac aattcttttg
     4801 agatagaaga cgttaatttt tgcggaacaa aaggttatga tttgaaagag gctgaccaaa
     4861 agattataaa tcgggaaata tcaaggttct caatttcata tcagagcatt aagaataaag
     4921 gaaaaaaaac aatagctgtt ttccattatc ctccagaaaa agagttggaa tatatgatga
     4981 aagaatatgg gatagagcaa tgtgtattcg gtcatttaca tggtttgaaa agcgaaagtt
     5041 atcaaaatga agggcataca ttggtttctg ctgattatgt aagattcact cctgttaaga
     5101 taatataagt aaaattatat tagtaatatg caatatttgt tagaattacc acattgtaca
     5161 atcattcaat ttaattatat aatagttgaa tgaaaaacca tctataatcg gaggtgtaag
     5221 ttgaatatat cacaggtaga aaaaactgct ttaagcatta gggataatat taacaaggta
     5281 attataggta aagatgaaat aatcgacctt gtgctggtgt cagttttatg taacggtcat
     5341 gttttattag aggatgtacc aggactggga aagacaatgc tggcgaaaag tcttgcaaaa
     5401 tcaatagatg caaaatttgc aagagttcag tttactccgg acttgctgcc ttcagatctg
     5461 actggtgtca gtatatataa ccagaaagaa ggagaattcg tttttaagaa aggtccttta
     5521 ttcactaata tagttcttgc agatgagata aacagagcaa ctccaagaac tcagtcagca
     5581 cttttagagt gtatggaaga acatcagata agcgcagacg gagttacgta taaacttgat
     5641 gagccatttt ttgttattgc tactcagaat ccattggaaa cacagggaac attcccactg
     5701 cctgaagctc agatggacag attctttatg aggctttcta taggatatcc taatgaacag
     5761 gaagagatag ccatattaag cagatttgaa aaggatatgc ctttaacaga ccttaaaagc
     5821 gtagcttcaa gagatgttat acttgaagca tctgaagctg taaacactat acgtgtaccg
     5881 gatgatataa aggattacat cgtaagactt gtagcagcta caaggacaag cactaagctt
     5941 atactcggat taagtccaag aggcagtctt gctcttatga gagcagcaag agcttatgca
     6001 ggaattcatg gaagagattt tgttattcct tctgatgtca aaaaggtagc tcctcatgta
     6061 atgggtcaca gggtaatgct taaaggtcat tcattatcat atggtgctaa gggaacaatg
     6121 gaagtaattg atgaaatctt gaacacggta ccagtaccaa cagaggaatt ataattaaag
     6181 catggaactg attctaatag tcatcagcct tgtaatattt tttactgcag agatactttt
     6241 ctttaaattt ttcgttttaa ggaatattga gtactctcta cagttcagcc aaagtgaagc
     6301 atatgaaggt gatgatatat tcatagatga agttattaat aataataaaa gagtaattgt
     6361 gccatggtta aaggctgata tacatgcttc aaaatggctt cagtttgcta ataccagctc
     6421 atcaattatt gatgaagaaa gatgcgtatc atcaggattc agtcttaacg gacgtcagag
     6481 aataaccaga agatggtata caaggtgtgc taaaagaggt gtattcacaa ttgaaaatac
     6541 gactcttaca gcaggtgatc ttgtaggaat aatgcaggat tcaaaagctt ttgtcgctaa
     6601 tacaagaata atggtatttc caggagtatt gaaacttgaa gaagcatttt tctcaaataa
     6661 ggaccttatg ggtgatatga tgataaaaag gttcatatta gatgatcctt ttatagtcaa
     6721 aggcactaga gaatatgtgc agggagaccc gttaaataaa cttaactgga aagctactgc
     6781 aagaatgcag aagcttatgg taaaggaaaa tgactttaca gctaaaaaag gtattacagt
     6841 attatttaat ataaaatcat atgccttgga atatattgat gtaaaataca aagatgttat
     6901 agagcttgga ataaaggtag ttaataccat aatggaaaga ggatatggta tgggattccc
     6961 tttgaggttc ggtactaatg ctgggattat atccaatcct gaagaaatga tattcagcga
     7021 ttctatagcg ggacctgaac atatgcggca tttaaaagta atccttgctg aacttatact
     7081 taaaaatcat ctggattttc cagagtatat tgaaaaacac tgtgatgaga tatttgatac
     7141 tcaggtatat attgtatcag catatttaaa tgatgaaata atagaaaaag ccgatatttt
     7201 gagaagaaga ggcaatactg ttaaattcct tatactaaac tacacttcag atgcagtctc
     7261 agacagaggt atggaagttt tcttgcttga taggaatacc gattttttga aggaggggga
     7321 atattatgga tctggaaaaa cagataaagc gtaaatttct gcctctttta ggtaatatag
     7381 gttctatagg agcattggct ttctctattt actatgctat atttcctgag tcagcggtat
     7441 tatggatggt gttctgtttc atagttatgc tagttggaac actaagcgga atgattatag
     7501 ttgaacaata tgaaaaaaaa gggaaagata tagtaagagc tgattatgat aatgtcagga
     7561 tacttctgca gcctgcatat atctttgctg taataccttt tgtgatatta ctattggtgg
     7621 gtttagtttt tacaggacag tttttaggat ctttcctcgg atatgctttc ctacctactg
     7681 ttttcttcat tcttggaatt ggaaatggaa tatttgatta tattcagaca gctcacagga
     7741 agatgcttta tgtagctctg ataccgcttg caatctcttt gtttgtagca tttatatcca
     7801 gtccgtttgg tttttatcag gatccggtat ggttatttgg ctcaatctat cttctcttct
     7861 ttttactagt tattaacaga ataaagcttg aagatatgtt tgtaaaaagc aagaaagtaa
     7921 atgtagaaaa caaaaaaaca ataagaagac gaaatgattt ctttatactc attttctttg
     7981 tcctttatct aataatattt gttataagaa atactttcac aaagatgtca gactggatta
     8041 tcaggacagc atttgctgta ataaactgga ttattaacca gttctataag ttatataagg
     8101 aaatagatgt agaagaagtc acacaggttg cagaagatgt aattgaagac gggccacctg
     8161 ctgtcacccc gcctttaaat cctgttgcac gtacaatttt tatagtaatc gcaagtcttg
     8221 taatagctat tgttatattt ttcttggtta aaaaaataat tcagctgata agatggatta
     8281 ctggaaaagt cagtgatact gtgagcacag gagtaagaaa gaaaaaagat gtagagaatg
     8341 aggagtttga agaaatagta gaagaccttg acaagaaaaa agagaagtcg aagtttagaa
     8401 gagctccaaa gttcaaatat accttaagtg gacttaaaca gctagatacg tataaagaga
     8461 aggtcagata cttgtatgga tttgctttgg aaaggcttat aattaaaaaa atagaaattt
     8521 caaaagctga tactcctgag cagatattag aaaaattaaa acagcaggaa catggcgatg
     8581 aatttgcaaa tgatatgttt gatgaattca ctgatgagta tataaatgtc cgttatggag
     8641 ataaggttaa aaaacaagag gattctcttg atgataaagt tgatatgctc gatcgtggaa
     8701 taaacaaaat caggccaaac aaaaaagaaa attaatcata ctttgcatat atatggtata
     8761 tttggtaaaa tcatatgtgc accaaaatcg gtcgtgacga tatcacgact ttaaaactta
     8821 aggaaattga tatggaagat attaaaaaag atatagaaga aataattgat ttaaataatg
     8881 atgatgataa agaattaact gggaaaattg agtatatgaa gataccatta atagctcttc
     8941 gcgctgttgc cgtaattcca agtatggtta cccattttga tgttggcagg gaaaaatcca
     9001 tacttgcttt ggaaaaagct atggaagaaa atggacaggt attccttatc atgcaaaaag
     9061 atgcttcagt agagaaccca aagaaagagg atctgtatga ttatggaaca atagctaatg
     9121 tgaaacaggt cttaaggctt ccaggtaaaa catgccgcat acttgttgaa ggagtaaaac
     9181 gtgcaaaact tgaggaagta ctttcgataa aaggttatca tacagctgtc gtatcttatg
     9241 tagatatgcc taaagccata atatccaaga gtaatgcttt agaggcaatg cacaggaagt
     9301 taacgactct ttatgaagaa tatgtcacat tgcttggcag aatatcacat gaaggttatt
     9361 ttagtgctgt tgcagctgat gatataggta cagttgcaga tgccatggtt ggttcaatgg
     9421 cattaaaatc tgaagtaata caatccttta tagaggaact tgacggaata gaacgtgcta
     9481 ataaattatt gcctcttttg gttaatgaaa ttgaaatact gaaacttgag agagatcttt
     9541 caacaaaagt aaaaagcact atagatgaga ggcaaaagga atattatctc agagaacagt
     9601 taaaggctat acaggaagaa cttggagaat acaatgagtc attggatgag gatgagatgt
     9661 cagagtatat caaagctctt gaatcagaca gataccctca ggaggtaaaa gcaaaactca
     9721 caaaggagat gaacaggctt tcaaagatgt ctccaatgtc accagaagca tctgtaatac
     9781 gttcatatat ggaaactctt gtggaacttc cttttggaat agagacaaaa gaaagactgg
     9841 atatcaagag ggtaagaaaa gtacttgagc gggatcatta cggtcttaat aaagtaaaag
     9901 agagaatagt ggaatatatt gcagcaaaga aactaagcaa tgattttaac tcacctatac
     9961 tatgcctagt tggacctcca ggaactggta agacttcaat tgtaaggtca attgctgaat
    10021 ccataaacag aaaatatgta agaatgtctt taggaggaat aaaagatgaa gcagaaatca
    10081 gaggacacag aagaacttac ataggctcca tgcctggacg agttgctaag gctattaaac
    10141 aggcaggttc gatgaatcct ctaatacttt ttgatgaaat agataaatta ggaaatgatt
    10201 ttagaggtga ccctgcaagc gcaatgcttg aagttcttga ttctgagcaa aacaattctt
    10261 ttagagatca ctatttagaa atatctttag atttaagtca tgtaatgttc ataactacag
    10321 ctaatactgt tgaaacaata cctcgtcctt tgcttgaccg tatggaagta atagaagtaa
    10381 caggttatac agaagaagag aaaatgcata tagcaaacaa gtacataata ccaaaacagt
    10441 taaaaaaaca tggtatgaca aaaaaacagc tttcaataac aaaagctgta cttgaaagag
    10501 ttataaatca ttatacaaga gagtcaggcg tacgtaattt agaaagagaa atagcatctc
    10561 tttgcagaaa agccgctaca aagatgcagc ttgatgagat tgatgaaata aaagtaactg
    10621 tagataatct ggaagaattt ttaggtccta agaagtatct ttatgatact acctttgacc
    10681 aggatgaaat aggtatggca agaggactgg cgtggactca ggttggtgga gatacattaa
    10741 ctattgaagt aaatattatg aacggcacag gtcatgttga gcttactggc agacttggtg
    10801 aggttatgaa ggaatcagcc aaagctgcta taggatatat acgctcacag acagacaaac
    10861 ttggtattaa tcctgacttc tataaagata aggatattca tgttcatgta cctgaaggag
    10921 ctgttccgaa ggacggacct tcagcaggta tcactctagc cacagcaatt atctcagctt
    10981 tatcaggcaa aaaggtaaaa agggatgttg ccatgacagg agagattacg ttgagaggaa
    11041 gagtgttgcc tataggtgga cttaaggaaa aactttctgc agcaagaaga gctaatgtta
    11101 aacaggtcct tatacctgta gaaaatgtca gagaccttgt agacgtacca caacagatac
    11161 ttgatgagat gcagataacg cctgtaaaaa atatggatga tgttttgaaa aacgctttat
    11221 ctgaatagat gttatagatt atttatttgg agtatttata cttaacagga caagctagga
    11281 tcttttgatt tttagcttgt cttttttcca ttttccagaa aagtagaagt atcctccaat
    11341 aagagatgca aatactgtag ctattggagc tgcgtaaccg agacttccga ctcctgtttc
    11401 caatactctt gcaaataaga aaacaagagg tatcctgaat actagcgaac ttacaatact
    11461 ggatgtcatg gagacaattg agtaacccga cccatttaat aatccattaa agcaaaaagc
    11521 gaatggcact attaaataat cataactaaa agatcttaga taactcaccc caatatctat
    11581 tacatctggt tctgctttga aagcttgaag aattgcttca ggaaatatct gaacaaaagc
    11641 gaaaatagct acacttatag caaatgagat ggttattgca gaccacaaag tcttttgagc
    11701 tctgtcatat aatcctgctc ctatgttctg agcagacatg gctgatactg atgcgctcat
    11761 agctatagca ggcagtatcg caaaagaatt gaatttgccc actatcccca cagcagccga
    11821 agctgtaact cctccaagat catttattat cgaagtcaga actaaaaatg aaatacctac
    11881 aattacattc tgagcacttg atggtatacc gacttttaaa agaagtttca atttatcttt
    11941 atgtatacga aaacttgtgt gatgaaagtt aaaaacaaac ctgcttcttt taagatatat
    12001 aattgctaaa aacatagctg ttgcctgtgc gattattgta gcaattgcag ctcctgtagc
    12061 attcatattt aaagggccaa caaaaattat atctagtata atgtttattg tgcttgcaat
    12121 accgacgaac gctaaaggcc tttttgaatc acccattcca cgaagtattg cacttatcgc
    12181 attataacca aaaacaaata ttattcccca catacagata agcacatagt tttttgccat
    12241 atcatatgat tcttctggtg tatttaaaag ctttaacatc acatcagaaa agaaagtcat
    12301 gaatatggaa aagaatacgg ctaaaatacc cataagtgta aagagagttc ctatagtttt
    12361 gtcaacttcc tcattttttt tggcccctat atactgagca ataagaactg taccgccaat
    12421 tgcaagtcct attacaaaat ttgttacgac cattgttatc tgaccgccta tatttacagc
    12481 tgacatggta taggtgccgc tgaaccagcc tacaatcagc atatccgcta cagaatacag
    12541 tgcctgaact atatttgaaa gtaataaagg aagactgaat tttatcagtt gttttataac
    12601 gcttccctct gttaatctat tttcgaaacc cataaaaatg tctccgtaag tgcaatatta
    12661 atatgttagc acaataggaa ctgagaaaca atagagagat tattagtgca attgcaattt
    12721 acaaaagccg atttagaata tataattact ctaattaagc aaggaggggt gctatatgaa
    12781 atccattgcc cagataaatg aaaagataaa aaaaggtaaa gctgtcgttg tgacagcaga
    12841 ggaagtaatt gatattgtaa aagaaaaagg ttataaacag gcatatgaat atgttgatgt
    12901 tgtaacaact ggtacattcg gaccaatgtg ctcatcaggt gtatttatta acttcggtca
    12961 ttctaatccc cccatacgaa tgcagagtat tatgctaaat gatgttccag catatggagg
    13021 attagcagct gttgatacat atatcggagc aactgaagta tccactaaaa gaggtatgga
    13081 atacggcggt gctcatgtta tagaggacct tatagctggt aaggatatta aacttcatgc
    13141 attatcaaat ggtactgact gttaccctaa caaggaagtt ctgacaacaa ttaataaaga
    13201 taatataaat caggcatata tgtttaatcc aagaaatgca tatcagaatt atcctgcagc
    13261 tacaaattca acaaacaaga ctatatacac atatatggga actctgcttc ctaactatgg
    13321 aaatataaca tacagtacat caggagaact tagcccgcta ataaatgacc cgtatctgcg
    13381 tacaatagga ttgggaacca gaatattttt aggcggaggt acaggatatg tgtcatggca
    13441 gggaacacag cacaaagttg atgtggaaag atctgagaat ggagttcctt tagttcattc
    13501 aggtactttg gctgttatag gcgacattaa gcagatgagc acaaaattca taagagcagc
    13561 agtatttgac aaatacggcg taacgctatt tgtgggaatt ggaataccta tacctatcat
    13621 tgatgaagaa atgcttaaat ttacatctgt aaaaaattct gaaatatata ccaaattggt
    13681 agataaatct ggtaatgtca gtattgacaa gaaattcaat tatgctgaat taagaagcgg
    13741 gactgttaag ttcaatggca agaatgtaca tactgcaccg ctttcatcca tatacatagc
    13801 aagaaacatt gcaactactt tgaaagagtg gataataaac aagtcatttg aactgaccca
    13861 gcctgttgct ccactgccgt ttgagccaaa ggttaattca ctggatataa gggagattaa
    13921 ataatgagca aacttaaatt aaaacttact tttgataaag attgtgtaac tagaccaata
    13981 atatatgatc ttgtaaaaaa gtatgatgtc atgactaata tacttaacgc aaatattgaa
    14041 tatgggaaca gaggaaatat gattcttgag attaagggta aggaagataa tctcaaactg
    14101 gcattggagt attttgacag tcttaaggtc aaatatgaaa tatatgcaga agcaatagta
    14161 tgggacgaag caagatgtat taactgcgga agctgtacag cagtttgtcc tactcaggcc
    14221 ctatatatgg gcaaggatga tttgcttcat tttgataaag acaaatgtgt agtatgtcag
    14281 ctttgcataa acgcgtgccc gttaaaaata atatcagtgg aattataggt ggagtaatgt
    14341 atcagaaaag ggattacaga aagtcttttt caaatgaatt aaaatggtac aacgtgtgtg
    14401 ttgaagaaac agaccttatg gtaggtacag atgaaaatca ttcaaaacag attcttaaaa
    14461 tgataagaac acttcgacat gatatatatg cagcaattga gaaaaatcag gattttttca
    14521 cttcactagt ccctattgat tatcccgggg cagaaggagt tgtcaagatg atggttgatg
    14581 cagctaaatt agcaaacgtc ggcccatttg cagcagttgc tggagcgatc aatgattcat
    14641 tagctaatga gctatccaaa atatcaaaac aggtatttat agaaaatggc ggagatttat
    14701 ttataatatc tgaaaaggaa aggaaaattg gcatatatgc cgggaattct gtactatcca
    14761 accggtttgc cgttgagata ccaaaagaag cttttcctgt cggcatatgc acttcatctg
    14821 gaactgtagg acattcattc agtatgggta aggccgacgc agcagtagtt atagctaaaa
    14881 catccgctat tgcagatgca tttgccactg ctttatgcaa caggataaaa aaggaaacag
    14941 atatagagcc tgctttggaa tgggttaatt catttgaaca ggtgactgct gctgtagcaa
    15001 tattggaaga caagattgga gcaataggaa atattaagct tgtgaaatgg aactgatatg
    15061 agaacaaagt taaaaaatgt acaaactata gtaataaaag taggaacaaa cactctgaca
    15121 cactcaaatg gtaagatgaa tctaaagagc atggagaagc tggtaaggga aatatcagag
    15181 ttgagcaatg aagggaaaaa agtaatttta gtaacttcag gagctgtcgg agtaggttta
    15241 ggcaagctta atcttgcaaa gaaacctaaa acccttgctg caagacaggt actggcttct
    15301 gttggacaat gcgagcttat gcacatatat tcaaagctgt tctctgaata tggcataaca
    15361 gtagggcaga tacttcttac aagagatgta actgaaaacc ctgctaaagc agagaatgca
    15421 tacaatacat ttaaagggct tttagatatg gggattgtac caatagtaaa tgaaaatgat
    15481 gccatctcaa cagaccagtt agctatagta gatacatttg gagacaatga cacattatca
    15541 gctatggtcg ctaaattagt tgaggcagat cttctgatta tattgtccaa tattgacggc
    15601 ctatatgata aagatcctaa tcagaatcag gatgcccata ttatagaaac tgtaactgat
    15661 gtagatgatg aaattcaatc ttatgcttct gaccttataa gttctttagg taaaggtggt
    15721 atgaagacaa agttatcagc agcaaagaca tgtatggaaa ataatattat tatggcgatt
    15781 ataaatggcc agaatatgaa gaacatatca aggttaatag atggggagaa tataggaacc
    15841 ctgtttatga aaaagaagta aagaaaggat aaacatgctt tatatagatg aattaggatt
    15901 aaaagcatta aaagcaaaag acgagcttgt ttctataaat tcaaaaatga aagatgaagc
    15961 tttaagggtt atggcgcagc agcttgttaa aaacagcagc acaatattat cagcaaataa
    16021 gctggatgtt atttgtgcaa gagaaagaaa agtcaaagaa tcactagtag acaggcttct
    16081 tcttactact tcaagaatag aaggaatggc taaagggctt attgaagctt ctcaatatcc
    16141 tgacccgata ggtaaagtac tttatgagac tgacatgcct cagggaatga aattaaaaaa
    16201 gataacagtt ccaatggggg taattggaat aatatatgaa tctcgtccaa atgttactgc
    16261 tgacgctgca ggcctgtgtt ttaaagctgg aagcgcagtt atactaagag gcgggtcaga
    16321 tgctataaac tcaaataagg cgattgtgag ggttttaaga aacgctctgg atatgttagg
    16381 aattaataat gacagtattc agcttgttga agatacaagc agggaaacgg caactgaaat
    16441 gatgagagct gttaaatata ttgatcttct ggttccaaga gggggagccg cccttattgc
    16501 ttcagtgatt gaaaatgcta ctgttcctgt tattgaaact ggtacgggca actgtcatgt
    16561 atacattgat aaagaatgtg acgagaaaaa agcagtagat attacaataa atgcgaagtg
    16621 ctcacgtccc ggggtatgta atgctattga gacattactg gtacataaag atgtatataa
    16681 aagagttctt ccaaaaatag aagaaaaact tttagaatac aatgttcaga taagggcatg
    16741 tgaaagagca aagcaggtgc taaatagctc agttgatgct acagaagaag actgggccac
    16801 agaatttctt gattttatac tagctgtcaa agttgtagac gacattgatg aggcaatcag
    16861 gcatataaat aaatatggta ctaaacattc tgaatgcata ataactgaaa tagaggaaaa
    16921 caaaaaattg tttacaaata agattgatgc agcctgcgta tatgtcaatg catcaacaag
    16981 atttaccgat ggcggtgaat ttggacttgg agcagaaata ggaatcagca cccagaagct
    17041 tcatgctaga ggccccatgg gtgctaatga gataacgaca tataaataca ttattgatgg
    17101 tcagggtcaa atcagataat taataaccat ctaaatgcag ttctggaaat tcagacagat
    17161 aaacaagcaa atcctcttca tatgagattg ttttgtctcc tattttaaaa ggtttgttaa
    17221 tgcggaacat ctgaccataa tcaaagaact taaaataaga tttatctgta gcaaagggag
    17281 aggatatcgc aatcatctta ggccagttca tctgattgca tatctgatag aagaaaggtt
    17341 tcatcatact tgtttcatct cctgctatat actcttttac cagaactccc tgatttgcct
    17401 taaggacgca atcagcatat gatacaggtt ttccatcttt taaaagatag tatgtattgg
    17461 taagttcgct acgtatccat ttttcatagt attcatcagt tcgaacatat acagggcaaa
    17521 gtttgtgtgc atgttcctca aaaagggttt ttatgacatc catacttttc agatctgctt
    17581 tttctaccga ataaccttct tggagctttg tttttctcaa actgtatact ttgtaatatg
    17641 aacatatgga aaaccatcca gctttaacat aatgatgatt agtgcctgta tgcaacatgg
    17701 ataaaggcat atcatgctta tccatatact caattgccat atgcataagt ttagaactta
    17761 agcccattcc ccgatggctc tttttagtgc atacttctcc gattccacca acttttatta
    17821 cagtatcctt aatttttagg taacgtgtaa ataccctgac agaacttgct atcccggttt
    17881 catctactgc tatgaatatt ccgtttatat ccctatatgg gtcattgctg tagtgacgca
    17941 taaagtatgc ttcacctaca ggaaatactg aaccgcaaaa ttgagtccat tgctgtagtt
    18001 cttctatttt caaagttcta aattccattt ttacctcttt aactgtaaat aatctaatta
    18061 gtaattgtta aacctattat cagcgtctta tcataacctg caaaatatat tgtaatgttg
    18121 caggttcctg cacctacagg acttacaata cctgatgagt taataaaagc tactgtagtt
    18181 ttactgctca tataggtaat gcttccctga agtgaagaaa gagccattat gttaccgtat
    18241 tggtctttag cttcgatata tgtgcctatc tggaaagttc cagatgaaac tgcttttgat
    18301 atattggctg aggtagattt cagtgttgtc agagtaggag cagtaccgga tgcagttaca
    18361 tcaatacttg taagttcctg acctgctttc cataacttaa cagttgtggt aattgttcca
    18421 gtcacatttt gagctttcag agtcttaact cctgaaacat cctcaattac aaaattattc
    18481 gctccatcaa catttgtaca tgtaatctgg tcaataccaa taggcatagt tccggtatat
    18541 gcaagattaa caagctgtcc tgaggcaagt tttcctgtga ctataatagg tttccagtat
    18601 ccgttatcag ttattgtatc tgttgcgcct ccgtacatgg ttgggatact ttgtatctga
    18661 tagcttacta tcttttcgtc ctcaactact aataacacca aatcataagt gctgattact
    18721 gcagaaccgt ttttaagagt aagtgttaat gcatactgac ctgccgtaga aggtgcggtc
    18781 atcgtgtacc cgccaccagt aactgttcct tcagtgaact ttgctcctga tggcccaaca
    18841 caagtaacta ctacggaatt tgaagctgtt aatgcaatat cattttcata ctggtcagca
    18901 atacttaaaa cacttgaatt aaacagaaca gtggagcctt tctgatatat ttgcgggata
    18961 tcaacaccga ttatatatgt tggtacagga gttgctaaaa ctgatatgtc caaagaaccc
    19021 tgtgaaacac cattatatga aatgtgtatt gtagcagttc cttcagaaga tccttttatc
    19081 tcaattcttt tagttgttga gttaaatcta atattagaat taggaattgt agtcgcattc
    19141 gtagatatca tcataagctt actgccatcc aaagtatcca tttgcataat attcccattc
    19201 tggtcttttg ctgttgcatt aagaggcacc cattggttta tatacaaatt accatcggtt
    19261 ccggcaatct ccaaagtttt tattaccgga ggagaagtta catttatctg aattgatgat
    19321 gatattgact ggccaacagc attcaatgtc actgtgcctt ccttgcttcc aacagctcct
    19381 gaaggtaaat acagtttgcc attagttact gtaatctttg tagcatcaat gatgagatta
    19441 tcggtagagg ctaatataac gggcttattt aatccaacat ctgatggggt tattgtatag
    19501 ttttgaccat attggtcttt tgcattaact gtaatgtaat gaccagatga gttaactaaa
    19561 attgatgtac tgcctgatgc atattcaatt tgtccaaatg agaatgaacc aattgctggt
    19621 gatgcgctca atgttatatc ctttgtaaca gatgttccag tcgaattttc aactataagc
    19681 aatctaacgg tgtcattagc ggctaagttc tgagcaccgg taagtataaa tccagctgat
    19741 gttttagaaa tagacagtaa gactgaaggt tttgcctggc aggaagcgtt gaaagtaaaa
    19801 ttagtataag ctgtaagtgg cagctgatta tcatattgat ttaaaagtat aactttaaga
    19861 tcctggttgt tcaatgcaaa tattctttca gcgcttataa ctatctcact gagtttttct
    19921 gcttcaactg tgaatgcttt tgttatattt gatacagtga cagaataact tcctggagtg
    19981 aatgctgttg atttggtaat tttcacagaa agtcttccag ttccccatga taaacttgtt
    20041 ccagtaacct gctgagtacc caaattcaat aaaacggtag ctccagatgg agcagctgca
    20101 ctgaatacaa cagttatttc cttagtccca gttggatatg cttcacttat tccaaaacta
    20161 tccggagtag gcgtaggtgt aggacttggt gttggggctt gcgtctgaga cggtgtaggc
    20221 gttggagtcg gagcctgcgt ctgagacgga gtaggcgttg gagtcggagc ctgagtctga
    20281 gacggagtag gtgttggagt cggagcttgc gtctgagacg gtgtaggnnn nnnnnnnnnn
    20341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    20401 nnnnnnnnnn nnnnnnnnnn nnnnnnnagt agcagacggg ctttgttctt cagatggaga
    20461 tggttcagct gtttgagtct gtgtttgtga aggtgtctgt gttggcggta aagtttttac
    20521 aggttttaca caagatgaca acatagcaac agacagcaca gtaacagcta ttattgttag
    20581 aatttttttt gttttcattt ttttatcccc cgcttattat gttatatatt agtatctgcc
    20641 atatgtttta gcagtttcta tcatagcgaa aagattatca tcaggagtat cacgcccaca
    20701 ttggtcccct gtggacaata tgaattttcc tccttctgca ccatcatcaa tagctctttt
    20761 tgatgcatcg atgacatctt gaacagtacc tcttagcatt acatcagtag tatgaagatt
    20821 tccttttaaa acaattttat ctccataaag tttttttaat tccttgaggt tgcagtctcc
    20881 cataggtggt atttcaagag gatctatgac tgttaattca gtttcttctg ccagcatttt
    20941 tactaaagca gtctctggtc cgcatgaatg aacatgagat ggtattccat attgcttgca
    21001 taactcagcc attcttttaa ctatagggaa agcaagttct ctgaatattt caggagtctg
    21061 gaatattaat gatccggaac ctccggtacc tataaaatcc ggtttatgtt ccagtgacat
    21121 aagtttatgg agtctttttt cgtaatactc catcaattgg tctctttttt catagtattt
    21181 atttgggttg tcgaaatagt catataccat ttctgagttt ttcaaaatta ttgatgtgcc
    21241 acaggttata cctactaagc catgatctcc catctcttct tttacccatt taagtaaagc
    21301 ttctcctgtt ggccattttg aaacatcctc gacaggctca tacttatcag gtatcagcgg
    21361 aagcttaacc aggtgcggat gaacatccca agaaggagga ttgtttttat ggtatacgct
    21421 tactaacggt tcccaaactt tttgattgtt ttcggtacgg tatttttgtg taactatttt
    21481 caaatcatct ttatgaacaa ttgcttctgt ccaggtatcg tcaaattctc caagttcttc
    21541 aaactgtata tttgcaaacc catccataag tgagtcgaaa ccaaaatatt ttacgctgtc
    21601 tatatatgct ttccactttg gtattctttg ataaaggtaa atgtcccaat atggcaaacc
    21661 agtgcgtctt atcggtatca tctgagatat atcaggcgct accggcacat gatctggtaa
    21721 ttcacctctt aatacagtta ataatcgctc ttttgaattc ataaacacct ctttgttgat
    21781 atagtaagca agttttcttt tattaaattt tcctgcttta tttattatat atcaatattc
    21841 cagttattaa aatatttaat tatctaaaat ggttatttgt gaacatctct tacttgctat
    21901 gatataatta gatggtatga atttagcact tttcatatgt gatgtaataa taaatacgga
    21961 ggatcggata tgataaaagc tccaaagaaa aatgtgaatc ttgcatttgt gggattaagt
    22021 aaaagaggaa atggactatt gaatatggtt ttagattcaa tggatgatgt aaatattaca
    22081 gcagtttgcg atgtgtatga agacagagta aataaagctg cactggatgt tttaaataaa
    22141 aggaaggcag aacctttaaa aactttggat tacaaagaac tactacacag aaaggatata
    22201 gatgctgtgg taatacctac atcatgggaa actcactttg atatctgtat agattttatg
    22261 gaagcaggaa agtatgcagc ttctgaagta ggaggagcta attcaatcga tcagtgctgg
    22321 cagctggtta agacatatga gaagactaaa actcctgtaa tgatactgga gaactgctgc
    22381 tatggtaaaa ctgagatgac tgttcttaac atggttaaaa agggtatttt cggtgagctt
    22441 atacatgctc agggaggtta tgaacatgac ctaagatctg aagttgcaat gggtaatcat
    22501 aacaggcatt acaggcttaa caactacaaa aacagaaatg gagatttata tccaacacat
    22561 gaacttggac ctatttcaaa atggcttgac ataaacagag gtaacagaat gctgtatctt
    22621 tgttcaatgt cttcaaaatc tgcaggcatt aatgaatgga tcagatccag aaaaggcaat
    22681 gattttgaaa atgctgattt caattttgct cttggcgata ttgttactac aatgattaaa
    22741 tgcgctcatg gagaaactat acttcttata catgatacat cattacccag accttattca
    22801 cgcggaaaca ggctgcaggg aacaaaagct atttggttgg aagataagat gtctttcttt
    22861 atagatggag taagtccagc aagtggatgg gatcatgaat gggaaagttt tgataagtat
    22921 tacgagcaat atgaacatcc tctatggcag tggtatagaa aaaaaggact tgtcggggga
    22981 catgatggaa tggattattt gtctatgaga gcttttatag aatctgtaaa gaatggtact
    23041 gatactccaa ttgatgcata tgatatggct gcgtggatga gcattacctg cctttcagag
    23101 caatcggttg ctatgggagg acatccagtg gcgatacctg attttaccaa tggaagatgg
    23161 ataaacaaaa gaccagttgt acaaagtaaa tattgtcttg atgaagtatg taatgatgaa
    23221 gtctatgagt aaaaatatag aaacaagtat aaaagactgg aagataggag tcacatcctc
    23281 tgggattaat catttatcga tagagaaata tcttgaatat aaaaaggcag ggatagatgc
    23341 tattgagctt tcatataatt tcgagaaaga tgaatccttc gatatttaca aacagaaaaa
    23401 attagctaag gaagcagaga taattttatg gtcatttcat cttccatggc atcatcagtt
    23461 atgcaatatc gctcatcttg acaaggatat gagaaatatc gctatagata tggacattca
    23521 aatgataaaa aagagtgccg actgcggtat caaatacggc gttattcatc cttcaggcga
    23581 acccatttta caagaagata gaaatgaagc tatgaaaaat gctaaagaga gcttgtacaa
    23641 gttgtcaaaa acagctgata aggaaggctt tacaattgca ttggaaaatc ttccaaggac
    23701 atgtttggga aactgttcct tggatatgaa ccagcttcta gaagcttccg attcgttaaa
    23761 aatatgtttt gatgtaaatc atcttctaca agagagtcat gcagagttta taaacaagat
    23821 aaaaggtaag attgttacgt tgcatatttc tgattatgat tttgtaaatg agagacactg
    23881 gatgcctggt gaaggaaaga ttaactggcc ggaacttatt gaacttcttg aaggtattgg
    23941 ttatgaaggt atattcatgt atgaattagg ccttaagtct gaaaaaacaa ttattagaag
    24001 agatttgaat tattctgatt ttaaaaataa ttttactgca cttagtgaaa aaagaaaacc
    24061 tgatgctata ggtaaaccaa acgtttaaag tatcacaaag gtttttcgaa ggcaaatcgt
    24121 tctttgccat ctaaaagata tataattccg caataaatga agccattctt gttaagcatc
    24181 ttttgcatag gtatgttatc tctgtgtgta tctattctta tgctttcagc attatttagt
    24241 tgagccatct gtttaccata attaattaaa taagatgcga tatttttccc tttataatca
    24301 tctcttactg caattctgtg tataacgcag tagggtttgt tatcagttat ccacactcca
    24361 tcataaattt tatcataggt cggttcgttt cctatgtata ttgcagctgt agctattatc
    24421 tgattgttgt tcttaactac ataacttatt tcttcatcaa tatcagacaa tataatctgt
    24481 tctgaaggat atccattttg ccactggtca acattcatct tttttaaagc agcctgtgcc
    24541 tgatgaaata tttccattag atatatcagg tcagactgtt ttgttttttc tatattgtac
    24601 atgagcaatt cctttattta aaatgggctc ctcttgtttt atgcaaatcg aaaagcatat
    24661 ttacaaggct tttatggtag ggtacgaatt tatcattgtc tataagatta agtatttcat
    24721 ttaaatctgc ccatttaaca gctgctactt cttctttttg gagaactaat ttgtttacat
    24781 caaaatcgtc attaataagg tagtaatcat caaaaccata tgtaaaatga atagttaaat
    24841 atggtctttg ttctgtcagg tcaagtttgt atccaatttc ttcaaatact tcgcgttcag
    24901 cacaagcctg gctgtcttct ccatgtaatg cgctgcctcc tactgttatg tcccaaagac
    24961 ccgaccatcc ctttttgtaa ggttgcctta actgtataag cattttgtta tctgaattga
    25021 aaatacatat atgagttaca atatggtaat cgccgttcat aaaaacatca ccgcgttttt
    25081 tactctttcc aagataattt ctattcttat cgtaaaggtc ccagatttcc ataaaaatcc
    25141 tttctgctat cttaattact gctatctata tctttttcat accatgcaag taaaatatct
    25201 gtaacttttc cataactttg cactccgtct gtttctccct gagctttcag gtatgcatca
    25261 tttgctttat ctgatatttc agctaccttg ccatcatacc tgtcccagtg ttcggagtat
    25321 ctgtcataat catttcgcaa gtggtcagaa tataattctg aaagctgtct atgagactgg
    25381 gcatcttttg aataaagaga attcatagaa aaaattaatg ccattgaata ggcgctgtat
    25441 ctcatattta tattatcaga ttttataagg gtgtagaaag cgaagaaatt agcttcgtct
    25501 tctgaagtaa tgccatttct atgggcaagt tcgtgagcac atgtaaaagg aacagcaatg
    25561 gctaagttgt tggaatttat attagcttct cctgtaagaa acgtgaaaat gccagtggtc
    25621 tgtgtatatg accaagcatt ggaggctatt acatatttag gtaaagcttc ctgtttattt
    25681 gtaaaatcag aaactgcttt tgcgctcaat ctggccattt cattaaactc attgcctgta
    25741 tagtagcctt gctcatttct ttttacctca gtagaaagct gatttgcttt ttcaatcaga
    25801 tatttattca aggcaataag ctcttctact tctctgtctt taacttccaa actcaactct
    25861 cccgtaaggc cgtcagaata gtacatgcat ccccacatgg aatagaagag gaaaataccg
    25921 atggataaaa ctaaaagcca tgctgttatg aacttaaaaa gccttcccag tcttcctcta
    25981 ttttttatta aatttttaat tagtattata agtgaaacag taagtattat agctaaaaga
    26041 tatatcagta tttctgctac agaaaactta acaaatgaaa atgcttttga catcgtatcc
    26101 atcatccaca ctgaccattt agaataaatg ccgtttgtaa acttaggtga gtttttggca
    26161 atgagtataa tgactatggt aaataatcca aagcacaatg atgcaacaag catctttaaa
    26221 gctttttttg tatcagtttt atttgttttc ctgcttttta tcttttttct catcttcaca
    26281 ttgtccgcta tacgggcatg cactgcatga accgcacgag ctgcaccctt tttttctgtt
    26341 ctttacaagg taccagatta ccaaaattac tattgcagcg acaattgcta ttataacata
    26401 atctattagt ttcatagtat ctcctaaaat aaaattgcta tccgatagaa aattaaagca
    26461 attacatatg ccaatgacgt ttgaagcaat actactacaa acatggattt tgtgcccaat
    26521 tctcttttaa cagcagcaat agctgcaacg catggcgtat agagaagggt aaacagtaag
    26581 aatgcaaatg ctgatatcgg atcaaatatt gtgcctaact ggctggatat actttctgtc
    26641 tgagtactta gtaatacagc aaaagtagag acaacggctt ctttagccat gaatccggtt
    26701 attaaagctg ttgaagccat ccaattgcca aatcccaaag gaatgaatat aggtgcgatg
    26761 aaagaaccta taagggccaa tatgctgtct ttgctgtttt caactgcgtt aaatcttatg
    26821 tcgaacttat ttaaaaacca tatgactatg gatgcaatga atataattgt aaatgctctt
    26881 gtaataaagt cctttgtttt ttcccataat aatagcatag tgtttttagc tgtaggtaat
    26941 ctgtaattag gtagttccat gacaaaaggt attggatttc ccttaaatac gaattttctg
    27001 gaaataagag cagctactat tgcaagtaat attcctataa catacatcgc tatcatgaca
    27061 acagctctgt atttagtgaa aaatgccata gcaaataccg agtatatagg aagttttgca
    27121 gaacagctca tgaaaggagt taggaaaact gtcatctttt tatccctgtc acttgtaagc
    27181 gttcttgtag ccatgattgc aggaacagta catccgaaac ctattagcat aggtactata
    27241 ctctttcctg aaagaccgat tttccttaac agcttatcca ttataaatgc tactctggcc
    27301 atatagccgg agtcttcaag caaagatagt aaaaagaaca agactatgat ggttggcaca
    27361 aaagataaaa cgcttccaac acctgcaaaa acgccatcta caaccaatga ttccacaaca
    27421 ggattaatgc cgaaattatt taagccttta gatacaacac tggctaatga gtcaataccg
    27481 cctgataaca gatcagaaag aaaactgcct actactgcga atgtaagcca gaatattaca
    27541 aacatgatac taagaaatat cggtatgcca aaaatcttat gagtaaggat gttatctatt
    27601 tttatggtct tgtcatgatg tttatcagtt tttggacgca caacagcttt ttgagtgatt
    27661 tcctcaatgt actgataacg catatcagca attgcagcca tacggtccat ggacaactca
    27721 ttttccattt caactattcc atgctctatc atatcttttt cgtttttgcc taaagacaaa
    27781 tcgttcaaca taggttcgtc cccttcaatt atcttaacag ccgcaaaaga agcaggaaca
    27841 gacgctcttt gcgcatgatc ttctaatatg tgtgctgtag catgaagggt tctgtgaact
    27901 ggtcctgagc agaaatcctg ccttggagga gcacatttgt tttttatgag atcaatcatt
    27961 gctttcataa gatcatcaat accttcgttt ttatttgcag atattggtat tatggggact
    28021 ttaagataat cacttaagat attagtcttt attactattc cattggcaaa aacctcatcc
    28081 atcatattaa tagctattac catgggaatg ttcagctgaa gtaactgcat tgaaagataa
    28141 aggcctcttt ctagtgaggt cccgtcaatg atattaatta ttccatcagg atgctctttt
    28201 aataaaaagt ctcttgccag tatttcttca cctgagtaag gggaaagaga atatatgcca
    28261 ggaagatcaa caagtttgac agatttgtca gattttgata ttccctcttt tacatcaact
    28321 gttactccag ggaaattgcc cacatgctga ttcgatccgg taagctgatt gaacagcgtt
    28381 gttttacctg agttctgatt acccgcaaga gctattatca tacagatacc tcctcgactt
    28441 caatcaagga agcatcttca agcctcaatg taagttcata acctcttaaa tatatgctta
    28501 ttggatctcc cataggagct tttctttgca tgtgtacaat aactcttggc gtaaggccca
    28561 tgtccaataa cctgtgtcga agcctgcctc tgcctcttac tgcaattatt cttccgtatt
    28621 tccctatagc aagtttatct aaagtcatat aaatatttct ccacatccgg cagatatttt
    28681 aactgccaat gatagaataa cttatgccct tcagcaagtc aataagcaat tgctaactcg
    28741 cttaaaaaaa tattactttt tgctgttatt tctttaaaac aggcaaatct attgacaata
    28801 tgtcttgcca tatgttgtat catgcatgta aacaaggcat aatatatata tggcatgtta
    28861 gccaaaaata aagaaggatt gaacatatga aattatttga aggatttaca tgtaaaggaa
    28921 aacttttatc aaatgatgac tttctaaaaa gacttagaaa cgatacggaa aaaggtattg
    28981 ataagctttc ggctgaagaa aaacaacact atatagataa tgctgaaaaa gctatagaaa
    29041 ctgaaatacc tattctcaaa gcgagtgttt acaaacagtt tgcatataca ggaaacagaa
    29101 cggaatatca aggtccatat tattcaagaa gaaatatgct tactgagctt attttcgcag
    29161 aacttttaga gaaaaagaaa aggttcactg agaaaatact tgatcttgtg tggctcatat
    29221 tagaagaaag tacctgggtt attcctgcac ataacaagaa tggctcatca ttgctttatg
    29281 aatttgatga tgtttcatat gttgatttat tttcagcatc aacagctgct gttcttgcgc
    29341 tgtcatatgt attttttaaa gaggatatga atgacggatt aactgatgat atgttcagta
    29401 aaagacttga gtatgaaatt gaaaacagaa tattacttcc ttttgagaaa tatgcaaaaa
    29461 actggtggta tataggtcac ataccaggac acaaacttaa taattggaat ccttggatac
    29521 attcaaatat tttatttatt ttagctatat ttgaaaaaaa tgaacaaaga aaagaaaaat
    29581 tatgtgatga catgctttca tatactgata attttttaga tacatacgga cctgatggag
    29641 gatgcgatga aggcccttct tactggggag ctgcaggtgc ttcatatttt gattttctag
    29701 atcttttata caaagcatca gatggaaaga ttgataaatt gtctgaaaag ctgatttata
    29761 acatgggtga ttacgttaga aaaatgtata ttggtggagg atatttcgtt aactttgcag
    29821 acagcgggcc aaaaccaaag aatcttgatg cagtgcttat atacagattt gccaaaagaa
    29881 cgggaaacaa gctgatgatg gattttgctt gttcatttga tcaatgcaat aggatatcat
    29941 acagccaccc tcaaagatct ttatataata tgattgaaaa gagtgaatgt aagcaatcgt
    30001 catataagcc gaataaagaa gagataatgg aagataccca agtatatgta tttagaaatg
    30061 atactcattt tatagctata aaaggtggac acaacagaga agcccacaat cataatgata
    30121 taggtcattt tgtgcttttt gaagaagaca caccgatact aattgatgca gggtctgata
    30181 catatagcaa gaagactttt tctgatgaaa gatacacatt atggaatatg cagtcttcat
    30241 ttcataatct tccagaaata tcaggatata tgcagctttt cggaattgaa tacaaagcca
    30301 gtgatgtcag ttatgataag aatagcaaat gtctttcaat gcagcttaag aatgcttatc
    30361 ccaaagaagc aaatatcaat tcatatatta gaaaacttta tgtaagaaat agtgagctga
    30421 ttatagaaga tgatattgat ttaaaagaag aaggggatat tacagaacat ctattgcttc
    30481 tgtatgaacc atcaataaga gataatttta taactgttaa aaatacacag atagctattg
    30541 ataatgttaa tgatataact tatgaaaaga tatcaacatt gaattataca ggactgaata
    30601 ccaatgttcc tgaaagaggg aatatggcta aatcatggga ttcagatttt atgttcagaa
    30661 taaaagcatc agctaaaaca aaaaatgcaa ctattaggtt tacaataaac aggagggact
    30721 aaatggcata tgaatggtat ggacactttt caaatgaagg aaaaaattac gtaattacac
    30781 aaccgaatac tccaaggcac tggtataact atatgtacaa tgatgagtat ataacctttt
    30841 catcacaggt aggttttgga gaaggttttg ctcaggacaa tatgggacga agggttccgt
    30901 tagttatgaa cagaaacttc tttttatgcg aagacgagta ctggtcatta gccggtttgc
    30961 ccataaatta cggttataca gattattcat gtacgcatac agctggttct actaccataa
    31021 atctcaggta taatgacata tcttcgtcag taaggatttt tgtaccgaat aacaaaatgc
    31081 aggagatttg gacaatatct ttgaagaatc attcaaacag acgaagagaa atttcactaa
    31141 taccatatgc aaagactgat gtagattctc catataagcc gcaaggatat aatatagcta
    31201 caggtgatgt atataaggaa aaaaactgcg tatacggatt cttttatcag gcatttaatt
    31261 cccaaagaaa cctgcccatg tatgggtata tgtcttccag tcagaaaata tcaggatttg
    31321 acacaagaaa aactgcattt gtaggtgcat attctgatga gcaaagacct gaggcaataa
    31381 ataacaattt aaagtgtacg aatagcagct gttttgctga aaaactatgt tttgcacttc
    31441 aaaacgacat agtacttgaa gcaaacgaac aaaagactct gcattttgtt ttaggagttt
    31501 ccttttcaaa agaagaaatt gtaacgcctg aagataagtg ggttcaacag cagtttcagg
    31561 ctatggaaga caaatacgaa cagaggatta gacaggttca gattaagaca ccatgggaga
    31621 actttgataa tcttgttaat ggatggttac catatgccac ggatatggga agcagatggg
    31681 caagagtcag acataacgga tacagggaca tgagtcagga tacagactgt ttttccgtaa
    31741 taaatcccag tcttgcatgg gaaagaataa aaagagtact tacatatcag tacgataacg
    31801 ggtatgctcc tcgtactatt attgacggag ttttaaaaga caggaatttt tctgacaata
    31861 cagtatggct tacatttgca gtgtatgaca tagtcaagga acttggagat ataaccatat
    31921 tgaatgagca ggtaaagttt aacaactcca aacaagcttc tgtttatgag catatacgaa
    31981 gatcagtaga ttttctctgg aattttacgg gactatacgg tttaataaga atatggggtg
    32041 gagactggaa tgactgcata aactatgcag gtctggaagg gaaaggagtg tctgtatggc
    32101 tgtcgatagc atggtgttta gctgctaaac aaatagctga actaagcggg atgctgggct
    32161 ttgatgacta tgaaaaatta tatcaagaaa gataccagat tatgaaagac agaatagacc
    32221 aacatggatg ggacggacaa tattatatat atgcaagaac agatgaggat atagttttag
    32281 ggtcacacac tcaagaagaa ggaaaaattt tcctaaatcc ccagatatgg gcagtttttg
    32341 caaacttaaa taaagataac aggcatataa aatctatgga taaagccgaa gagctcttgc
    32401 agtcagatga aggtattctt ttatcaaaac ctccgtacac aaaacagtat gactatattg
    32461 gctcaatggc tgaaaaacca ggcggtattc aggataacgg aggagtttat ctgcatcctg
    32521 cagcatggaa gcttgcagcg gactctatat taaagaacaa tggaagggtt gaggaaaacc
    32581 tgaagaagat gcttccattt gataatgact attacgaaac gaaaggtgaa ccttattgta
    32641 tgtttaactg ttattatctt gattatcacg gttacagaag aggaacaccc ggacagtcat
    32701 ggagaaccgg tacaaatgcc tggctgttaa aaagtactgt tatgtatata ttcgggcttc
    32761 accctgaaat ggaaggatta aggcttgaac catgtcttcc tgaatcatgg agagaatgtt
    32821 ctataactaa gaaattcagg aactgcaaat atatagttaa atatatacaa acagaagatg
    32881 gagcatgtaa caatatatca aaaatactag ttgatgagga agaaaaggat tttgataatt
    32941 tcataattcc acctgtggaa ggtaaacagc ttaatattac agtgttgctg acgaagtaat
    33001 atagacgcta tatttagtaa catagcaata tgtaaaatgt tgcctatcca aaatattatt
    33061 tttctaatat ctttataagt tgagacggat ggtctattac tgtttgagct ccaagttcat
    33121 ataaaatgga tttgtctctg aatccccacg aaactcctat aaactttaat cctgaattag
    33181 aagctgtctt tgcatcaaca tcagaatcac ctatgtacaa agcctgtgat ttatcacatc
    33241 ctaatttttc tagtgccaaa tatacgctgt cgggagcagg tttctttttt attttttccg
    33301 attctcctat tgctacatct atatagctgt taaaatactt gggagatatc tgttttacag
    33361 catcatcaaa cttattagat acaatagctg tttgataacc ctttactttt actttattta
    33421 ataaatcaag tatcccgtca taaggcttag tcttgttttc catgttggct tggtaatgag
    33481 ttttgaaagt atcaagacat ttttgaaaat caggattttc tctgccgttt attaaagata
    33541 gttctaaata tctttctgca ccatttccaa catagcttct tgcctgttgt attgaaattt
    33601 cttcatatcc aaaagaagat agagcataat tcatgctgtc agtaagatcc tcaagcgtgt
    33661 taagtaatgt tccgtcaagg tcaaatatta aggcctttat atccataaaa cctccgttaa
    33721 atgtaatcaa gtattttatc cagtgaatct ataactggta tattttcatc ataattagtt
    33781 gaagcttcaa gccagttttc gctgtttctt ccatttgctc tgtaccagca ggcatccata
    33841 ttggcattaa gtgctcctac tatatctgtt gtatacaaat ctccgataaa aagacagtct
    33901 gtattattta aattcatttt gtgaagcata tagtcataaa attctttcat cggttttaca
    33961 cagttcattt cttcagatat gaaaagatga gacagatact gttccaccgg ttgtattcgt
    34021 tttctctggc tgtctgataa tccgtttgta gctgctgcaa gtatatgagt ttttgatgtc
    34081 ttttttaata tctcaacagc acaattttca tatatgcgag tagtattaaa gatatcaatg
    34141 tactcatcag atagaatttt tggatttgta tcattcaaag aactatactt catatgctca
    34201 caaaatactt cgaagcgttt gtaattatac aaatcgaata actgatgata atttttcctt
    34261 atgccttcat cattaagcat attcaaatca aaagctttcc ataatttata gcagatatct
    34321 ctaaataggt tttctatttc attagtgtat tgaatgtcat atttttctaa cagggctttt
    34381 agtgaagatg cggcgcatgc atcataatta aggatagtat tatcaatatc aaatattaag
    34441 catttatatt tactcaaggt tcagttccgt ttcttttaaa tttgtactgc tgttaccggc
    34501 atatattatg aatgttccag gttccactgt ccatttctta tctttccaaa aagaaaaatc
    34561 tcttggtctc aatgtgaagc ataatgtctt tgtttcattt ggtagaagtt ccaccttgcg
    34621 aaatctcttt aattcaataa ctggccgtac cagtgaagcc acaaggtcac gaatatatat
    34681 ttgtacggtt tcctcacctt taacatctga ggtatttgtc acagtaactg atactgggat
    34741 atcatcattt acagtatatc tttctttttc aataatcgga gcggatatat caaaagatga
    34801 ataagataaa ccgaatccga atggataaag aggttctact gcagatttat aataacttgg
    34861 agccctgcct ggccagtatc tgttatagtg gcactgtatc tgtccaacgt ttcttggtat
    34921 ggttacggga agttttgcag aaggattatt ttcaccgaag actgtcagtg ctacggcatt
    34981 accgcattcc tgaccaagat agaagcccaa aagtatagca tcagcattat cttttgccca
    35041 ttcaaatgag cttggtcgac catccagtat tactagtatt agttttttag aaacttttct
    35101 gactttttca aaaagcatct gctggtcttt ttttaatgca agatcatcga catcctcatt
    35161 ttccatgcag gtaatttcat tgcctccaag aaccagtatc gttacatcag aagatttagc
    35221 ttttctgact gcatctggaa tatcaattgc tgaataatcg ttttggtcaa gtgcctgaga
    35281 atatgatacc tcaaaattgc cttttgcata ttttttgatg ccatctaaaa ctgatatata
    35341 gtatttaggt ttatctgttg agtaattgcc taatagtaat ctgtcagcat ttggtcctat
    35401 gacagccatc ttattaatgc tgtttttatt aagaggaaga accgagttct cattttttag
    35461 cataataact gcttttaatg ctgcctttaa agcgagagca tcatgttctt tgttgtaaca
    35521 tacagtgtct ctgtcctttc ttggtttgcc aaaatataaa gtttttcctg ccatggaaac
    35581 atgctcttgt cctgaattag ttgaatagca tttttccaaa gattcctgct caggatctgc
    35641 agtaggatca tctaaaagac ccagttcata tttctttata agaacccgtt taacagcttt
    35701 attaattttt tcaatatcaa ttttcccatt ttcaacagca tttttcagaa caggaccgta
    35761 aagcctgttt tcattccagg gaatcatcca tgcaagttct gtgtctactc ctgcattgac
    35821 tgccattact gcgctttgtt cagaatcatt ggacacatat aaagctgtat gaagtttcct
    35881 tatatctcca ttatctgata ccacaatacc atcaaattta tattcatcac gaagtatttc
    35941 ctgcaaaaga tacttgttgc aatgacaagg aacaccattc aaactgtgat gtgctggcat
    36001 aacagaacat acatggcctt cctttactat agtttcaaaa ggaagcagat gtacttctct
    36061 cagacttctt tcagaaattt ccattgcagc tccatttaat ccagctaaag gttctccatc
    36121 agcaacaaaa tgcttaacag tagcaatgat gtgatcttta tcaagatatt tatctgaggt
    36181 gccctgtaaa cctaatgtga aagctagacc gattcttgaa acatgatatg catcttcacc
    36241 atatgcttcc tgaatacgtc cccaccgagg atctctcact acagcaacca taggtccgta
    36301 acactgatgt attccacaag ctctacactc ttttgcaata actgttgcaa cctgttctat
    36361 gagctgaaca tcccatgttg aacctaatgc taaagcctga gggaatgatg ttgcatatgg
    36421 agtaacagcg ccatgaagac attctccctg ctgcatatga ggtatagata atctggcatt
    36481 tccattggga aaagaagctg ccatctgatg caatttttca tcaagagtca ttactgctaa
    36541 gatgctgtca gcccattttt ctgcttttat tcttctttcc agattgaatc tgtccatttt
    36601 tggaacctct tcttctgatg atttatgtca tcattttaat tttatcaaat aagtgaaaca
    36661 taaacaatga aatatggtat tttgatgttg actttacatc agacctaacg gataattacg
    36721 caagaagggt tgaaggaggc gaaaaatatg acagaggaag ccgggcatat attaaccaac
    36781 aaacctgtac cgaatgatat atctgatgaa atcaaaaagt tgactgataa tgagcgcctg
    36841 ctatttacaa tagtgggcga tttgtctaaa aacagcaagt atagccagtc agtgcttgca
    36901 gttacagatg aagcaatgat atgttttgat gagacatacg aaaataagta tctttatcta
    36961 aaacatgggg atatatctga tatatatatc aaaaggcttt atggaaatgc gataatgctt
    37021 gttaagacag aagaagagta ttttgaattc tttcgtttta cctattctgt agcgaattta
    37081 tgcgaagcag cagcagattt ttctaaaaag ttaaaactga aagaagatac catgaaagct
    37141 atggaggctg ttgaggctgt ttttgaaaaa cagttcagta catgtccaaa atgcggaaga
    37201 acccttgtta ggcctggtgc tgaatgtatg aactgtcagc caaaagggaa gatagcacaa
    37261 aaactgatga agtatatcgc accgcaaaaa aagctactta taatctgtct tatattctca
    37321 ttagtaacaa ctgcaatgtc tttagtaccg ccatatataa caaagatgat ggttgatgat
    37381 ataattccaa agaataataa aagtcttctt ataacggttg tatttgttct tttaggtaca
    37441 tatgtaattc aatattccgt aggagcctta cggtcatatc tgttaagaat gagcggggac
    37501 aggattgttg caggcctaag aagcgatatt tatgcgaaag cccagtatct tcctatgaag
    37561 ttttatgata agacttcaac cggttcagtt attacaagga taagcagtga tacatccaca
    37621 ttgcagtctt tcaccctgag gataacgcag gaagtaattg tacaattttt ccttttgata
    37681 ggaattataa ttataatgtt tgtaatgaac tggaaactga cattattgtc tcttatgcca
    37741 gtcccattgg ttgtggtagg ctcaaggatt ttcggaaaga agattttacc atactacaga
    37801 agaatatgga gaaaatggtc tgcagtgtct tctatactta ctgatacaat tcccggtgta
    37861 agggttatca aagcatttac aaatgaaaaa ggagctatag ataagtttga caggtataac
    37921 gaggagtggt taaaaactga tataaaggct gcaagaatta ctactctttt tccacagatt
    37981 gtgagtttct ttgttacctg cggatctctt cttatctgga gtgttggagg aaatcttgtt
    38041 atgaacagcc aacagggttt gactttagga ttgctagttt cctttatttc atatacttca
    38101 atgttttatg gtccagttaa cttttttgcg aattttaacg attcgcttca atcagctatt
    38161 gcagctgcag aaagagtatt tgatataatt gatgctgaac cggaacctga ttttggaaaa
    38221 ggcagtcatc ctaaaaaatt gaatggaaag atacagttta aaaatgttaa cttctcattt
    38281 gatcgtacta aaatgacttt gaaaaacata aatgttgata tagaaccagg tgacatagtc
    38341 gggattgtcg gaactactgg ttcaggaaaa tccactttaa ttaatttact aatgagatat
    38401 tatgacaact atgagggaca gatattaata gatggtaagg atatcaagga agttgacatg
    38461 gaattttaca gatctcagat gggatatgtt cagcaagaac ccatgatgtt ccatgatacg
    38521 atatataata atattgcata tggtttggga aatgttcagg ttgaacaggt aataaatgca
    38581 gcaaaaattg ctaatgctca caatttcatt ataaaactac ctgacgcata tgatactgtg
    38641 ttaggagaaa gaggtactgg tctttccgga ggagaacgtc aaagagtatc aattgcacga
    38701 gccgttttaa gaaatccaag cataatgata ttcgatgaag cgacagcggc agttgattca
    38761 gaaaccgaaa tgcttataca agaagcaatt gagagactta ttgccggaag aacaactcta
    38821 atgattgcac acaggctttc cacacttaga aaagcaaata agattcttgt agtggatgaa
    38881 gggcagatta tcgaatgcgg ttcacctgat gaattactag cccaaaaagg caaatactat
    38941 aaacttatag agatacagac aatgtcttct aaactgcaga aagcaaaaga agaggaaaaa
    39001 cttgaataga ggtataaaat gagatcacca tacatagaag gagataatgc gaaaataaca
    39061 agaaaagacc tcacacacgt taatgtcctt atgtataacg gacaggaaat tcttgatgta
    39121 gaaccaagaa ggttatttcc cataagcggt cttagaaaat atattacatt actggataag
    39181 gatgaaaaag aagttgctat aatcagaaat cttgattcgt taatggaaga atcaaaaaca
    39241 gcagtagagc agtgtcttga tgagtattac cttatcccta aaattcaaaa gatttacgaa
    39301 ataaaggaaa tatcaagaaa catgaatatg tatgttctta ctgacaaggg ggaaagacgt
    39361 tttgagatag caaacaggca taatgatatc aaggtaatac atggcacaag agtgttgttc
    39421 agggatatca gtgataacag atatgaaatt gaagatgcgg ataagttaga taaatcaagt
    39481 aagatacgtc ttggggctta tatatgatat aatttaaata tggagggaaa aatgaaactg
    39541 atacttgcaa ttgtcaataa tgatgacagc gcgatagcag cctcggcatt aacagaggca
    39601 ggatattttg taacaaaatt atcaacaacc ggaggatttc taatggttgg caatacaacg
    39661 ttacttattg ggacagaaga tacagaaact gaaaatgtca ttgaaatatt atccaaatat
    39721 tgcaaaactc ggaaacaggc aacaccttca acagcttcat ttggaaatgg gttatcaaat
    39781 gagagcctgc cgcctgaagt tacagttggt ggagcagcaa tattcgtaat gaacgtagaa
    39841 aaggatatta aaatatagtt ttcctgttat aaaagagaga atatagacat ataataatat
    39901 aaacatatat tttaagcagg aaaataactc aggatgatta aatcatcctt tctttttagc
    39961 ctttttagat atagaagatg ttgtcatgtt ttttgttttc tttactgaca tatcatcttt
    40021 ttttgtgaat atccatataa atgcaatacc cactaacaat cctagagcag cagctgcagc
    40081 atatagaacc atgtaaataa gagctgtata gttatagtaa atgaatgctg cagggatgaa
    40141 cagcacagat gggaaaagaa tatacagcca tttataccta ttgtacaaag cgaatattat
    40201 gcttgcaata taagtgactg ccggataacc tagcaaaagc atgtacaaat taaatccttc
    40261 catattagcg cttataaaag cagctggaag cagattaaac ataaatgcat ataatgctat
    40321 atgtatgata tagtgatttt taagtctgtt agctttttgt agatcgctca ttttttcata
    40381 ttttcttttc ataaatagat tttatagaca atactaatct attgcaagaa gaaaaggtaa
    40441 atgttaattt caaattactt tgattttgtg ttaaattata tatgtaccat aaaaatacgg
    40501 aggtaaagtc atgtgtgata ctttgggatg tgtaaaccag aatgttccca tatttgggaa
    40561 gaatagtgac agatgtgttg gagaacccca gcttttagaa ttttatcctc gaagaaataa
    40621 tattgaaaaa acatttatgg ctacatacat agaggttgaa caggcagaac aaactaatgc
    40681 aatacttatt tcccggccgt catggatgtt cggagcagag atgggtatta atgaatatgg
    40741 tgttgtaata ggtaatgaag cagtttttac aaaaggcgga tataataaaa tcggacttac
    40801 aggaatggat cttttaagaa ttgctcttga acgttcggct gatgcaaaac aggcattgca
    40861 acttattatt acttatcttg aaaaatacgg acaaggcggt aactgcgctt gtgaaggtga
    40921 aaaatattat aataactcat ttcttataat ggacaaaaaa gaactttatg tacttgagac
    40981 atatgataag caatgggctt acagaaaagc tgaaaaggac agtatatcaa acaggctgtg
    41041 tatagggact gaaggggagt gttcaacagg caaagagtgt aattttgcca aaatacatac
    41101 tgagcctgtt tatacatatt tatgtggatc aggacacagg agaaatcgca cacagacatg
    41161 tctggaacag aaatgtgact taaatggttt taggaaggct ttaagaagcc atactaaggt
    41221 taaagatcct ttcaactctg ggacggctaa cagtgtttgt atgcatgcag gaggtgtggt
    41281 ggataatcag actactgcat ctattattgt gcagctttat ccaactgcgg taatatgggc
    41341 aactggttct tcattgccat gtgtttcttt atacaagcca tatcttttcg ggacaaaagc
    41401 tccttttgta tttgcaccag atgatacttc atcagttaag tactggaagg agagggaaac
    41461 ctttaacaga agaatgctgg gaaattctcc tccaaaggaa tattacgatt atatttatga
    41521 actggaaaat caatgggaag agtcagttaa gttaaattat gctgatgcaa aagccatgga
    41581 agaaatttca aataaagctt ttgagcagga acagcagttt tttgaaaaaa tggataagat
    41641 aatctcaaaa actccaaaac caacattctg gcatatgggt ttttggaaca aaagggaagt
    41701 gtaatcagcg gttatttatt ttcttccact catctttcaa taatgagtag catgatatat
    41761 ccattggttt gttatcatag gaaggccttg cttttctgag tgtaccttca tatttgaatc
    41821 cgcatttctc tataaccctc atagatcttt tgttatcagg ataatgatca acagaaagaa
    41881 gatccagttc caaaatctca aatgcatatc tctgtacggc ttttacagct tcggttgcga
    41941 tacctaagcc ccagtagtta tatgacaaag cataaccaag gcactttgct tttaatgatt
    42001 ttactgttga acgcagcaaa tcactgtgca aaccaattga acctatgacc ttattgtttc
    42061 ttctatacac aatagcccac acatcatcat ttctcataaa catattaagt acagcttctg
    42121 attcacacag gcttttatgt ggagtccagc cagcattcgg gcctacattt ggatttttcg
    42181 cgtactcata taaatcctct acatcatcaa atgtgaattc gcgtaatatg agtctgtctg
    42241 tttctatggt atccagcatc tgtacctcca ctatatcttt attatattta acccttcatt
    42301 aagtattttc ttaatagtgc caactgaaaa tacagcattt acatttttag gtatctgaat
    42361 gcgatattca gttatttggt cacttttcat atatgatact gctatttttc catgtctgca
    42421 agttatatgt ccttttgctc tatttaaacc ttcaatcttt aaaggcgaaa tgacaacttc
    42481 atcaaatcca gatgtatctg gtttctgttg tatacccaat acatattcaa acaggtatct
    42541 tgcagaggct ccaaacattg gatgattatt cgaatcacca ttccatcttt cccataatgt
    42601 tgttgcacca tgttttttca tgttataaaa agaagcttcc tttttgttag ctaaaagcaa
    42661 gaaagcaaga tctgataatc cgttctcaaa aagaactttg ttcaatatat atgtgccaaa
    42721 tattccagtg tcatatgtaa gcaatgattc atatcttctt tttatattct taagtgttct
    42781 ttcatcacct aaaaaaatgt catatgcaaa agcgtctgca ccctgattgc caaatataaa
    42841 tgtgctctcg agctttgaca tatatgcatc agttattgct tttttatgta tctgctctag
    42901 ttttgatatt ctttttttgt cttcaaactt ctttaaaata tctgcagtag tctgcccaat
    42961 ctgaagcagc tttacataaa gacatgtgtt tacgtaactt tctggtatat acgtaggttc
    43021 aggtgtacac cattcaccta agcaccaacc tttttcctcc tctttaacta caagcccatt
    43081 cgaacacctg ctttccatgt attcaaaata atgtagcatt ttgggaaaga attttcttaa
    43141 aactgataag tcgccataat gcagataata ttcatatgga actgttacta ttgcccctcc
    43201 ccatccagaa ggacctccgc ctcctccatt gaaaggagct gtatgttgaa catgtccgtt
    43261 gtttatatcc tgacagtcag aaatatcttc aagccatttt ctgtatactt ttttcatatc
    43321 caaacaatac atagctgcac gggcacataa ctgcccgtct ccagtatagc ctaatctttc
    43381 tctgtgaggg cagtcagaag gtacgcttcc atgaagattg gtaagctgtg accttatata
    43441 tgctttgtaa taccagttaa ggattgatga actgcaagaa aaagaggaat ttactttaac
    43501 atctgtatgt actatatgac attctaatgc tgttgcatta tcagtaacag taaaatatct
    43561 gaaaccctgc catgtgaaaa ttggattaaa ggtattggta attccatctg tgatatattt
    43621 cattgtacac ttttggcctt tacctcctcc catagaaatg aaattcaaag tattatcatc
    43681 attaattctc tctgcacatt ctacctgtat atgttcattt ttcttttcgc agtatattac
    43741 aggataacct gatatgttta tacctgcatc ataaacaaaa agcccatctt tatggtctat
    43801 cagctttggt ttaattttct taataatttt gtctttattg cagttttgaa tatataattc
    43861 actttgtggt atatctgttt caatactgtc aagccagcct gatgtgtctg cattttcagt
    43921 gttccaaaga taatcaaagc cattgtaatt atgctcttcg ccataaaaaa gattagaaga
    43981 tttgatgaac ccttccttat acataacgga tttgtctgat tctatatatt catcatctaa
    44041 tgagagtcta aaacaaagct tgatacgccc atatgtaaca tttccttcac caattctttt
    44101 tgtctggtta tagaaaccgg aacccaacat tatgcctaaa cagttaatgc cgtccttaag
    44161 atactctgtc acgtcatatt tcaaacagta tatcctgtga ttcatagtat cactaatagg
    44221 atatctgaat tttttcgtat ctcttggatg ataatctgaa taagcaggag tgaaaagatc
    44281 gtcgcttact tttttcccat ttatatacag caaaaaatat ccaagaccgg tgatagtcat
    44341 tactgctttc ttgcatcctt tattaataaa tgtctttctg aatatggctg aatccatatc
    44401 cattgaaggc attatccaat gtgcattttt gaatatagtc ttatgataca taaagtctcc
    44461 aaatacatat tttttctaat agaaatatta tacacgaaaa acaagagtaa ggaataataa
    44521 catgcattat ccttattgac taatgtatga gtgtgacata aaatcctctt atggaggttt
    44581 gaaatgaaag caaaggatgt tgaaaaagct aaaaaagaag ctttattaat gtataataaa
    44641 gcacatatag ttttaactga aaaggaaaaa gaagatattg agatagctga tttcggatta
    44701 gatgatttct acaaaactgg tttaagtatt gtgacatatg ttaatactga caactgttgt
    44761 gcaaaggaaa tggttcttca tccatttcaa acatgcccag aacacataca tgctccaata
    44821 gaagaaatta aatacaaagg aaaagaagaa accttcaggg ttagatatgg aaaagtatat
    44881 ctttatgttg aaggtgaaac atcaaagaaa gtatctgtta aaattccaga tgaatattat
    44941 actgtttttc atgagataga attaaaccct ggagagcagt ataccttgta tcccaataca
    45001 tggcattggt ttcaggcagg tgaaaaaggt gctgttattt cagaattttc cacttccagc
    45061 catgatgaat gtgatcactt cagaaatcct aatattgaaa gaataccaac agtagaatag
    45121 ttatcttaat aaaccatttt tcattaaatg aaggttattt gtaagtatgg tatctattcc
    45181 catatctaaa aattcacaag cttctttagg atcatctgac cagaaaatat tgcacttaat
    45241 gttatttgca tgagcctggt caatcatttc ctgagtgaag taaggtttat agaactgcag
    45301 ttttttgcaa ccgaactgaa tagctttgtc aacaatagaa taatcctgct gtcctgccaa
    45361 acagcaacgg gaaagatgag gagctatttt tctagcagtt tccaaaactg cttcatctcc
    45421 ggcaatatat acatattctt cacatccata ttccttaatc aggttataaa tgattctgaa
    45481 agcttcctca tcatatgggt gtatatttac accattgata aatttagttt ttatatgaat
    45541 attcataata gtctgatgat tgaatttcct aagtatttct tcaaatgtgt tgtatctcaa
    45601 cccttcaagt tgtgaactca tctgataaga tgcatccagt ttcattatgt catcaaacgc
    45661 atattcccag acctttctct gagaattttt ctcaaacaca ggatcatgtg ttgcaattat
    45721 ttcataatcc tcggaaggcc ataaatcaaa ttcaacttcg tcggcatcta aagcaattgc
    45781 tgaagctagt gatagaacgc tattttcagg acatagtgct gaaaaccctc tatgggcaca
    45841 tattctaggg tagggttttt cgttgtcatt cagagaaatg aaacttcctg ctggtctgta
    45901 agaatatgga gttctgcctt gtgttataaa ttcatctgaa tctatcaatc cctgtccatg
    45961 accattggat cttttgcatt tgttgctaat gtctacatga ttatccaaaa tcccaactga
    46021 ttgtttaata tcattcaata tcttgccgct gcaatcacat acaagagtat gacctcctgt
    46081 aagtttgtct cccatagagt aagaggaacg aaggacataa ctgttacatc tggaggatat
    46141 catttttacc tgattgaaaa tattatcatc agattcactt ctttggtaag atgatattaa
    46201 tattaggtca ggttttttgg aagctatata ttcaatatat tctaaaaagt atgtatcata
    46261 gcaggtcata aatgcaattt tcataccatt aacttcaatg cagcaaggta tatacggacg
    46321 agtcttatat gaatagtcga gtttgagtat ttctttttcg gcatatgtta gatgctgttt
    46381 atcatatctg aaaattacct ttccatcgga gttatataga aaagtgctgt ttctgtatcc
    46441 tgtcttagtg tcataagcaa tatttacggc tattagtgcc tctgttcttt ttgctgttga
    46501 aataattttc tcaatgagta tagaagtatt gttcttgtca tcttcaatca tttccttaag
    46561 agttgatgct cctgggatat tggaaaactc aggaagcaga atcagatcag catcttcagt
    46621 gatgttatca agcgagttga tgatgaaatt aaatgtttct ttcttgtcag attttgtttt
    46681 agggtaagca ggctggatta gatatgtttt cataatttcc ctttcgtatt ctcttcatta
    46741 tttaaaagca gttccattct tctgtacttg gttttaaaat tatgtttttc ataaaatttc
    46801 attgcatctt cattgaattc ccacacatta agctctattg cggagcattc agcagattct
    46861 gcaaaatttt ttgcatgatt tataagcaaa gaacctattc ctatgcctct tttgttatcg
    46921 tctatacata tgtcatcaat gcaaagaatc ttccgctctt tcaatactgc atgttcttta
    46981 cgaatctcaa aatagcaaaa tatgtatcca cagactttat ctaagttgtc aacagcgaca
    47041 aaaatccgct cttcagggtt ttttatttta aagtgaacaa aatcatgggt atacttattt
    47101 gcatttgatt tgaatatatc cggtcttcct atatgatgca gttgagctat ttgttttaga
    47161 agccttacta tatcctctac atcttttttt tcggctgttc ttatggcata ttccatatta
    47221 cgccttcttt cttaataact tgtaaaaaat cattatacac attatatctt aaacttatag
    47281 ctcagacaaa caaaatctgt tcttttaaat tgttttacat gctgtgttct ttatgtttag
    47341 gtatataata tatatattaa attttgagag gtatgtatga ttatcagtaa taaatacttt
    47401 aaagatggca aacccaaagc ccttactatg agttatgatg atggacctta tgaagattat
    47461 agattagtag aattatttaa taaatctgga ataaaagcaa cttttcatct taactcaaga
    47521 tatcttgaca gagatgatta tccgccacaa ccagaaaaga ttaaaatgaa agatgttaag
    47581 aatctgtatg aagggcatga gatatcggta catactgtta atcactgtga cttaagagct
    47641 gtaaacaata acaggtttat gcatgaaatt gtagacgaca aagcagccct agagaagctg
    47701 gccggttatc ctgtaagagg aatgtcatat ccatatggtt attataatga tgatgctgta
    47761 agtcatttga aatactttgg gatggaatat tcaagaacca caaaaagtca taaatccttt
    47821 tatatgccag atgattttct ggtatggcat ccatcatgtc atcatattga tgccatagaa
    47881 ctcatttctt ccttcgatag cccaaggtgg aacatggaaa tgcctttgtt ttatgtatgg
    47941 ggacatagct atgagttttc aagatttggt gataagtggt acttaattga agatttctgt
    48001 aaaaggataa gcggaagaga ggatatatgg tatgccacaa atattgaaat taaagattat
    48061 tcagatgctg tgaaaaattt aaaattcagc gctgatgatt cgatggttct gaataacagt
    48121 tgcattgatg tttggataga tgtcgataga caacctgtaa aaataccaaa aggggagctt
    48181 gttaagctta agtgatatca atacataatt ttgaaagtgt taagattgta gtctatgatg
    48241 ccttcttctt tcattttgct taattctctg gaaagagctg ctctgtcaga attcagataa
    48301 tcagccagct cgtttcggga taatggaata ttgaaatttc tgttttttgt ttttgttgca
    48361 taaatattaa gatatgcaag tatcttctct ttcagagatc gtttagaaag tatttctagt
    48421 ttctgatgta attgaagatt ctttgaagca agttgattca tcatattttc aataagtctt
    48481 gaatgaaact cacatgcctt attacaagat tgaattatcc ttggaaattt taaccacatt
    48541 atcacgcaat tagcagttgc agaaattgtc actggtgatt tggatatgtt tgcacaaaca
    48601 aaagcttctg caaatatctc atatttttcc gctacaccaa taacagacct ggttcccaga
    48661 aaatcctctg ttgttattaa aacagctccc tgtacaacaa ggccaatgct ttcaagagaa
    48721 tcatcagtca ttagtattat ttcattcttc ctgtaagtta cggtttttgc accaagacat
    48781 gatagcatgc tcttaaggca gtcaggttca atgtttctga aaagctcaac attttttgat
    48841 aaatcttcat ataatggggt tgtcattttt caccttgtaa cggtttaata tatataaaat
    48901 attaaactat atatgttgca attgcaacag acgattatgt ctataagtga taaatttgca
    48961 tagaagacta tttatgctgt ttaaatgaaa ggtatataag tgactatgat aagaaagatt
    49021 ataaaaattg atgaggaaaa atgtaatgga tgcgggattt gcgcgaaagc ttgccatgaa
    49081 ggtgcgattg gtatggttaa caaaaaagca aaactaattc gggatgatta ttgtgacgga
    49141 ttaggggatt gtcttcctgc atgccctaca ggcgctatta cttttgaaca aagagaagca
    49201 gaaccatata atcaggctgc tgtagatgca aagaaaaaaa gagaagagaa agcaagagca
    49261 gttcctcagg cttcatgcag ttgtatggga agcgtagcaa aaacacatga tagaaatgtt
    49321 caagaaacca gttcatgtga atcatgtact cctttaacaa gcagactcag gcagtggccg
    49381 gttcagctaa agcttgtacc tgttgaagct tcgttcttca acaatgcaca tttacttata
    49441 gctgctgatt gtgcagcata tgcttatgcc aattttcatg aggattttat aaaaaacagg
    49501 ataaccgtaa tagggtgtcc taaacttgat tctgtacagt attcagaaaa attaacagaa
    49561 atactaaaac gtaataatat taaaagtgta actatcgtac gcatggaagt gccatgttgt
    49621 ggaggattgg aatacgcagc taaagaagct ttgaaatcat gcgataaaat gataccatgg
    49681 cagtcagtta ccatatcatc tgatggaaga atactggatt agttatatgg ttaatagata
    49741 tagattttgt actagataag taaattgcta ttatgaagat ataatcaaca ttacttaaat
    49801 agaaaatata attaaaggag agaaaaaaat gaaagtaaca attgatcaag acggatgcat
    49861 aggctgtgga ctctgtgcag atacatgccc aagtgtattt tgtatgaatg atgacggcgt
    49921 agcagaagta tgtggagaag tagatgaaac taatgaagat ttagtaagag aagcagcaga
    49981 aggatgtcct gtttctgtta ttgaagtaga agaataagga ggattgtata tgtcagatat
    50041 gttttgtttc cagtgcgaac agacagctaa aaactttgca tgtgaaggta aaaaaggagt
    50101 ctgcggaaaa gaagcaagca cttcaaattt acaggatgag ttaacaggag cccttatagg
    50161 gttagctaga gcagctgtat caaacggctc gtatatatca actgacaggg ttatgcttga
    50221 aggtttgttt acaacaataa ccaatgtgaa ctttgatgat gaatctatta ataaacttat
    50281 tgataaagtt catgatgaaa aaaatcttct tgcacctcaa tgcgcattct gttcatcaga
    50341 ctgttcaaag agtgaggatt atgatgtatc taatctttgg aaagataatg aagatatccg
    50401 ctcattgaaa tcgttgatac tgcttggttt aagaggtatg gcagcgtatg catatcatgc
    50461 aatggtttta ggctatacag atgatgaagt aactgaattt atatacaaag caatgttcgt
    50521 gttaggcgaa gactgggata tgaatgatct tctgcctata gttatggata cagggaaggt
    50581 taacttaaaa tgcatggaac ttctggacaa ggctaatacg gaaacttatg gtactccagt
    50641 tccgataaaa gttccaatga caattgaaaa aggccctttt attgtaatat ctggacatga
    50701 tttaaaagat ttgcatattc ttctaaagca gacagaagga aaaggaatca acatttatac
    50761 tcatggtgag atgcttcctg ctcatggata tccaaagctt aaagcattca ctcaccttaa
    50821 aggtaatttc ggtacagcat ggcagaacca gcaaaaggaa tttaaagata ttcctgctcc
    50881 tgtattattt actaccaatt gtataatgcc tgtaaaggat aattatgctg ataggatttt
    50941 tacaactgaa gttgtccagt atcccaatat ggttcatata ggggaggaca aggactttac
    51001 cttagttata aataaagcgc ttgaattagg tggatacaaa gaagaccatg ttataaaagg
    51061 gattaatggc ggttctgttc tgacaacagg ctttggtcat ggtacggttt tatctgtagc
    51121 tgacaaagta gttgatgctg ttaaacagaa taaaataagt catttcttct tagttggcgg
    51181 ttgtgatggg gcaaaaacag gacgtaacta ttacacgaat tttgtaaagc aggctcctaa
    51241 agattccata attcttactt tagcttgcgg gaaatacaga ttcaatgatt tggatttagg
    51301 tgatattgaa ggcttaccaa gaataatgga tatgggtcag tgtaatgacg catacagtgc
    51361 aataaaagtg gctgtagccc tttctgaagc attcggatgt agtgttaatg aacttcctct
    51421 tacattggtg ctttcgtggt atgagcaaaa agctgtatgc atactgttaa cattacttgc
    51481 cttaggtata aagaatatat atttagggcc tacactacca tcttttattt cttccaatgt
    51541 actggattat ctggttaaaa atttctcagt ttccccgata tccacacctg aagaggatct
    51601 taagaagata ttaggataat caggatatta aaaaggaata ctcggtaatt caattttata
    51661 tgtagttcac ttatttaata aatgtagcgg agtgaattac taaatggatt aagagcaaag
    51721 gaaccggtta tggttccttt gctttagcta atattacgat tttattcatt gtttttttaa
    51781 tctatctgcc atatgagctt aacatatttt tttgagcaaa taccattttc catgcaatag
    51841 aaggtgttta tgtaatcttc atctttatcg tggatttctc ttgatattac tagattgttt
    51901 tcgtcattat acttatgtgt ttcaactatg atgtaatctg tgtctgaatc aagagatata
    51961 ttatctggca cttcatctgg aacaaaatta ttgtgcctta caattgaatt attcttatcc
    52021 atctgataaa tacttatctt aaaaggtgag tgtatatatg agatagatat tttgactgaa
    52081 gacttttgag tcttggttat tccgttttct gttatagttt tactatcttc cagagtttgt
    52141 gagaatactg agccttcaac ttctgtctga cctgagatat atccgctgcc agttactgca
    52201 tatacatcac catttgatga ctgataaact ggacttatga aaaatgctct attagttgag
    52261 gtaattgata cataaatcgt tccttctaaa ttaatttctt tctgagtatt gccacctgta
    52321 actttcgtat gaccatcaga aataccatcg cttgaacttg ctaaggtata aacgccgtca
    52381 ctgtcagaaa tagttgctgc aatatatgta ataccttcta tatcttcaaa tacatactca
    52441 gatgattctg aagtctgatt agtctcttca tttgtatgtg tttttgttac aagctttgca
    52501 taaagtcggc cttcatattt gcttatatct atgtcctggc cattttcgct cataattcta
    52561 tcgatattct cgttgatata tgaatcgcta tcaaatagaa tgagcggttc taatgtaatc
    52621 agtgctccaa tcaatatatc ttcggttgca atttcctgca tatcttctat cgccagctga
    52681 catcctgcga gtgacactaa aattgctatt aatagcgcta ttatgagtat tttcttactc
    52741 ttcatcttta tcatcctcca aatttccatc aaacttcaat atatctccta cgtcacaatt
    52801 gagatagtga catattttat ttacagtatt aaatcttata ccctttgctt taccacttcg
    52861 aagaatagat aagttggctt cagttatacc tatcaaagag cataattctt ttgaggtcat
    52921 tccgcgttct tttaagatat cttccaagtg gactaatatt gccatttatc tttctccttg
    52981 tatattaata ttagacagaa tcatggcatt ctgttaaatg cttctt
//
LOCUS       DAOSXM010000007        52683 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2111
            NODE_405_length_52683_cov_12.467204, whole genome shotgun sequence.
ACCESSION   DAOSXM010000007 DAOSXM010000000
VERSION     DAOSXM010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864178
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 52683)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 52683)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:24:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,261
            CDSs (total)                      :: 2,229
            Genes (coding)                    :: 2,218
            CDSs (with protein)               :: 2,218
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 3 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52683
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_405_length_52683_cov_12.467204"
                     /isolate="AMR_MDS_2111"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            97..3609
                     /gene="nifJ"
                     /locus_tag="PLQ68_02045"
     CDS             97..3609
                     /gene="nifJ"
                     /locus_tag="PLQ68_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014062313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HXK71460.1"
                     /translation="MGKKLKTMDGNQAAAYVSYAFTDVAAIYPITPSSPMAENVDEWA
                     AHGQKNMFGQEVRVVELQSEAGAAGTVHGSLQAGALTTTYTASQGLLLMIPNMYKIAG
                     ELLPSVFHVSARAVAGHALSIFGDHSDVYATRQTGFAMLAAGSIQEVMDLAGVAHLAT
                     IKSRVPFVHFFDGFRTSHELQKVEVIDYADFDKLVDKEAIKDFRNRALNPSSPVLRGT
                     AQNPDVYFQAKEASNRFYDAVPAIVEDYMKEISKVTGRTYNLFDYHGAKDADRVIIAM
                     GSVTDTAEEVVDYLVEKGEKVGLLKVRLYRPFSIKHFLNALPKTVKRIAVLDRAKEPG
                     ATGEPLYLDVVSAYKGDKEAPIIVGGRYGLGSKDTVPSQIASVYANLALNEPKNNFTI
                     GINDDVTYLSLPVAKKINTTPKSTVSCKFWGLGSDGTVGANKNAIKIIGDHTDMYAQA
                     YFAYDSKKSGGITVSHLRFGKSPIKSTYLVSDADFVACHNQSYVDKYDIVSDVKKGGT
                     FLLNTGWTVEEMEERLPGQMKRYIANNDVKFYTIDAVKIAQELGLGGRTNMILMSAFF
                     KISNIIPIDDAVKYMKDAVLKSYGKKGEKVVNMNNAAVDKGIENIVEVKVPATWKEAV
                     DLPKEDKKYPEFITNVVEVMNAQQGDSLPVSTFVGIEDGTWQQGTARYEKRGIAVEVP
                     EWNIDNCIQCNQCSYVCPHAAIRPFLVNEEEQKNAPEGFETKKAVGKGLDGITFRIQV
                     SPYDCAGCGNCAQVCPSKEKALVMKPIDTQMNQAVNWEYATKLSVKETPFNKYSVKGS
                     QFNMPYLEFSGACPGCGETPYMKLITQLFGDRMMIANATGCSSIWGGSAPATPYCMDC
                     NGNGPAWANSLFEDNAEFGFGMYIATRHMRERIEELLKQLLESDACDCVKEAAQNWLD
                     NKNNGETTRKASEQLLEKLGCCEKNDIVNELLDKKNYFVKTSQWISGGDGWAYDIGYG
                     GLDHVLASGEDVNVIVYDTEVYSNTGGQASKSTPTAAVAKFAAAGKKVKKKDLGMMAM
                     SYGYVYVAQVAMGADQNQFIKAVKEAEAYDGPSLIIAYAPCINHGIRTGMGTTQLQEK
                     KAVEAGYWHLYRYNPMLAEEGKNPFVLDSKEPTGSFKDFLASETRYTSLKVTFPELAD
                     DLFEKTEKDAKLRYQTYKKLSEQE"
     gene            3733..4254
                     /locus_tag="PLQ68_02050"
     CDS             3733..4254
                     /locus_tag="PLQ68_02050"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HXK71461.1"
                     /translation="MLNLRITDSDILNEGEPQYVSRISKYGSRGVLLNNKGQIALMYV
                     SDSDIYKLPGGGIEEDETEEQAFIREIKEETGYHCHILSYLGIVEEHKAKKDFLHISH
                     CYYAETIGKKGSLKLTKEEKKRGYKPHWFDLNLAIEILEECFRKSIGYRTLFVLKRDL
                     AILYYVKGLTKNE"
     gene            4247..6520
                     /locus_tag="PLQ68_02055"
     CDS             4247..6520
                     /locus_tag="PLQ68_02055"
                     /inference="COORDINATES: protein motif:HMM:NF014851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HXK71462.1"
                     /translation="MNNITLNGKWNLYFTEEKDAYIDKPSQLENSNYRCIEAEVPGNV
                     EADLVRTSIEKDPYVNQNSFDYMKYEYYQWWYTRTVDIPNNLNGDKYILLFNGIDTIA
                     DIYLDDILIGHTENMLVEHEFDVTNLITPSNTYTLSVRIYSVMNYIRRKEYSVWMRGC
                     RHCSELPYIRKAPHMMGWDILPRLVSAGIWKDVKIIAVKNTRITQAYYATPVFSMDKI
                     RMRFAYRFTTDHDDLSGFMIRVKGICEDSEFEYTIPSWFVSGNDGFLIDKPKLWWPRG
                     YGEQNLYTVTMELLYNNNVVDTRTDIIGLRTFRLERRFEKGNQEFKIFVNETPIFING
                     TNHIALDILHSKDHLRQRKEIELAAECNCNMIRCWGGNVYPETDFYNLCDQYGILVWQ
                     DFTFGNSNYPQTEEFFNTAYEEAEKVIKKFRNHASLVLWCGDNEIDTTLDGYWYPDYK
                     SKHNRISSEVLEKAVQQHDPFRIYLKSSPEIPDGFDSYNVPEQHIWGPRAYFKDDFYK
                     HVSAKFIAEAGYHGCPGLESLKKYIPKEDLWPIEGNKTWAHHSTEDYLIEDIIGRRNT
                     LMANQVRVLVGKLPDTLETFITVSQVSQAEAFKFFIERTRIKKWDMTGILWWNLLDGW
                     PGISDAVVDYYFNKKLAFDVIRRVQEPVCVMLDEIKGWERAVYIANDTNSDKSVNYKV
                     YEYQDNLVVVEGDTFIERGKNKKINEFFEIPGTKKLYIIEWTVDNKIYKNHYMAGYPS
                     FDTDTILKWYKVIKELD"
     gene            6584..7744
                     /locus_tag="PLQ68_02060"
     CDS             6584..7744
                     /locus_tag="PLQ68_02060"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HXK71463.1"
                     /translation="MDNMEKKIRVGLLGIGGRARHFADLVRQYPKTELAAVCDISEEA
                     MARSVEEKNLHDIPQYTDYMTMLNEESLDVVFVCTPIEYHCEHTIMALEKGVHVVGEV
                     PVAFNIEQAKQMVQAVRKSKAKYFFGENMCFAKHVLIVRQMVLAGLLGELHYAEGEYL
                     HDLKKLAEFTPWRFDTLFGTNGVTYGTHSLGPILSWFDWDRVKKVMCSGSGSRFRYAD
                     GRPFGMESTTVMLCKTEKDRLIKIKNDLSSPRPSINQYVLSGTKGAFESRRGIPDSKD
                     YIWIQDIVGSSEQRPRSFHDNSDTWLPLSIFESRYFPQIYKEWVWYAKTYGGHEGADA
                     FMFISVVDSIINNKEPYIDIHRGLDISLPGMMSTISIQNNSEWVDVPDSRLW"
     gene            complement(7745..8953)
                     /locus_tag="PLQ68_02065"
     CDS             complement(7745..8953)
                     /locus_tag="PLQ68_02065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK71464.1"
                     /translation="MKVKLTLDEPVIVSLGPTVEEAGWGTHQFPAINTDDNGNIGVTF
                     HGAKDLIEAYGTEPCLYISKDKGKTWEEVDSKDVHNTKTRFGIKLPNKDRYTMETRTP
                     VKVDTSKLPEPMPFQHDTIKLYPAEGVSDEMLDKRWRYLVLSEGQDVPEYKYFDYDKK
                     GLCITSSYDALVPPFPFGRIRIDHENTVWQTTYRRSRNPDNLGFSPYYNTHYYTFSQN
                     YNYLYQASWIPYIPDTRIFKNSFVTEGFAESDIAFLPDGSCITLMRTGSDTPSFLARS
                     TDKGRTWSKPVVFDDKGVWPCLCVLPCNVTLASYGRPGFYIRATADPTGLSWDAPVEL
                     ITSNDRTCERNTPISIYNQTSRRGTCSYSDMIALNENTAMVVYSDFFVPDEFGIKRKA
                     ILTRKIHVDI"
     gene            9165..9518
                     /gene="fliO"
                     /locus_tag="PLQ68_02070"
     CDS             9165..9518
                     /gene="fliO"
                     /locus_tag="PLQ68_02070"
                     /inference="COORDINATES: protein motif:HMM:NF016250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HXK71465.1"
                     /translation="MEFEDFLPLILGLFIICGVLLAFYWILKKMTGKANRLSAGKYAR
                     IIDRIAISQDKQIQLVEIGEYVLVIGISSSSVNLLYKVKKENLKQFEQDISNPQFKDI
                     FSNFLSRSKDNERKL"
     gene            9518..10276
                     /gene="fliP"
                     /locus_tag="PLQ68_02075"
     CDS             9518..10276
                     /gene="fliP"
                     /locus_tag="PLQ68_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019424998.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HXK71466.1"
                     /translation="MAKKRKIGIFATLLVAFTPFSVIAAEDDLTEVLEQGLDVLSGYS
                     NLLPTVIILLLLTVLPTILLLMTSYTRILVVLSFTRNAMGTQQMPPNQVLIGISLILT
                     IFIMSPTIGQIKDVAYDPYVNEEMTIVQALEAAEMPLKEFMFNEAQTEPESINMFLSL
                     SGVEETPETLEEMPMSVLIPAFITGELTKAFKIGFMIYLPFIMIDMIVSSILMSMGMM
                     MLPPSMISLPFKVLLFVLVDGWELIMKTLIMSFG"
     gene            10294..10560
                     /gene="fliQ"
                     /locus_tag="PLQ68_02080"
     CDS             10294..10560
                     /gene="fliQ"
                     /locus_tag="PLQ68_02080"
                     /inference="COORDINATES: protein motif:HMM:TIGR01402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HXK71467.1"
                     /translation="MDQALVVNLIKDALTVVLKVVLPVMIIAMVTGIIIAVFQAATQI
                     NEQNMVLVPKIVVVFLGLILLGSWMIGQITEYARELFNQILSLI"
     gene            10568..11347
                     /locus_tag="PLQ68_02085"
     CDS             10568..11347
                     /locus_tag="PLQ68_02085"
                     /inference="COORDINATES: protein motif:HMM:NF013478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HXK71468.1"
                     /translation="MNSLSVVYTNIDVFLLVLVRIASFIFVSPVFGRRGIPVTTKIGL
                     TAVLSVVIMLGFETFPDVAEMGSGMYLGLVIKEALIGISISFITLIFFTVFYGIGQLA
                     DMQIGFNVGGIYDAQMQMQVPLTGNLFYLMGFLTFLVLNGASKLVFLIHSMYDSIPIV
                     GSSLNSTFYEIFFNAFTFTYAYSVQIILPLILLILVTQFILGVIIKFLPQMNVFIIGI
                     PVKIAISLIILNFMIGPLFQMMEVYFDRMFESAMMLIRSLS"
     gene            11347..12399
                     /gene="flhB"
                     /locus_tag="PLQ68_02090"
     CDS             11347..12399
                     /gene="flhB"
                     /locus_tag="PLQ68_02090"
                     /inference="COORDINATES: protein motif:HMM:TIGR00328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HXK71469.1"
                     /translation="MAGEKTEKATLKKRRDAKKEGDVHKSIEVSNTFSLLVITLLVGV
                     YLSHAGTSLVLFLQSSLYITEIEVVFDFRKYLKLFFDTAGVFMIALLAMGLLTNYMQV
                     GFLFTSKKLKPDLKKMNPIEGLKRIFSKKTLFELFKNLGKMALIIILSYSALNKAMLE
                     FSYLSFYEILPALHKALSVMISILTSILIGLATLAVIDFIYQWFDYEKKMKMTKQEVK
                     DEFKNTEGDPQIKSFIKSRQRMIARRRMMHAVPTADAIITNPTHIAIAIKYKHKKDTA
                     PIVVAKGQGLIAEKIKQIAKENNIYIVENKPLARILFKKAEIGDFIPYEHYKAVAEIL
                     AYVYNIKNKKEQGDQN"
     gene            12386..14443
                     /gene="flhA"
                     /locus_tag="PLQ68_02095"
     CDS             12386..14443
                     /gene="flhA"
                     /locus_tag="PLQ68_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006316262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HXK71470.1"
                     /translation="MIKIKSKFKQSILALIVIGVIMIIVVPLSTTLLDILLIISITFS
                     LIILLTTLYVKEPLQFSIFPMLLLITTLFRLALNISSTRIILSEGGIAGKVITAFGNF
                     VVQGNIVVGLVIYLIIMIMQFIVITKGAERVSEVAARFTLDSMPGKQMAIDADLNAGL
                     INEKEAKERRKKIEYQADFYGSMDGATKFVKGDAIAGIIITLINLVGGIVIGLTSEGM
                     DFSQVLEVYSLATVGDGLVSQIPSLMISTATGIVATRSSSDQDLNEQFSHQLFSDYKV
                     LFITGGALLLLSIVPGFPGFFLVTIGGLLLALGYAIYRKTKVVPENEHEKEEIQARTE
                     REPVKTNIMELLNVDPIKIELGYSLIPFIDESQGGDLLERMITIRNQFAGNMGIVIPV
                     VRFRDNLSLKSNEYSIMIRNNVIEKGVVLADYMLAMSSSSVEEADLEGIDTKDPAYGM
                     SAKWITKDMQDKAQMLGYTVISPSSVIATHISKVIKKHAYEIIGRQDIQKLVDNLKER
                     QSVLVEEVIGKTVSIGQLHKVVIGLLKEGIPVKSLDVIVETLGDYAKTTKDINVLIEY
                     VRQALKREITQIYAPNGRIDVITVNHDIEDMVTKSIRSTESGGYLALEPDKIKHIIKA
                     ANESINKAVTANIDAVILTSPIVRIYFTEIIHQIDENIDVLSYNEIENKVKIQIIGSI
                     SLQ"
     gene            14453..15184
                     /locus_tag="PLQ68_02100"
     CDS             14453..15184
                     /locus_tag="PLQ68_02100"
                     /inference="COORDINATES: protein motif:HMM:NF005413.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HXK71471.1"
                     /translation="MDKTKIWKEYINTQTVSSRNLIVEQYAYLVKKVIYSIPCTRIGM
                     IEEDDLYSEGVIGLIDAVEKFDPDKNVKFESYACLRIKGQILDYMRKVDILSRDARKK
                     TKEVADYINTYINRYGKEPSLSEISSSLNISVDKVKRIQEEDALSNMVSFESFIEDNG
                     DIIQSKKEEEYPEEALDKSQLLEAMAYHIDKLSEKEKTILNLYYYEELTYKEIAEIMD
                     LCESRISQIISSVLANLREKLINNI"
     gene            15240..15956
                     /locus_tag="PLQ68_02105"
     CDS             15240..15956
                     /locus_tag="PLQ68_02105"
                     /inference="COORDINATES: protein motif:HMM:TIGR03506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein"
                     /protein_id="HXK71472.1"
                     /translation="MDIGLQQLGKSLLATNSHLAVISNNIANSATDRFKSDTLSIRSF
                     SDYFIHEDHISNKENLYEITTHTDFTSGEIYKRNSNDFALDGNGFIAVIYNDNLCYSR
                     GGSFNVDADGYLVDQHGSYILGTQGNILVNNANVEISKDGTVTLDGIYADTIEISDFE
                     DYRLVNKTGKDYYVAEQGANVLQSNTNLLQGYSEKSNVSITSEYLRMIEMSKKFETGQ
                     AVIQILDEINGKAVNNIAVF"
     gene            15965..16714
                     /locus_tag="PLQ68_02110"
     CDS             15965..16714
                     /locus_tag="PLQ68_02110"
                     /inference="COORDINATES: protein motif:HMM:TIGR03506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body protein"
                     /protein_id="HXK71473.1"
                     /translation="MNNAYYIAANSMMSQEKVFSTISGNIANINTNGYKKTSVSFSDL
                     MYSFQQTASSSEYSIGSGITVNGYNKDFQYGNLKETGSNLDIAFEKEGFLTLADGDER
                     YYTRGGNFTLSATGEGYFISNNDGFFLLDSEGNRVQISQEERDFEIDSNGLMSFKNSD
                     RIVNLAITSFENPEKLQPFGNNLYIAGLELKENTQTADIILRQGFVEQSNVDIATEMT
                     DLIQAQRLYQMNSKILQVMDEISGMANRLRK"
     gene            16729..17007
                     /locus_tag="PLQ68_02115"
     CDS             16729..17007
                     /locus_tag="PLQ68_02115"
                     /inference="COORDINATES: protein motif:HMM:NF016223.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HXK71474.1"
                     /translation="MKINPLSSYDAINKVNKNAEKSQITKTDMNVNDDVRISDQAKAI
                     DKYIQKAKTSDIDRSELVNSLKNKVRSGNYKVDSKVLSEKIIESVVKK"
     gene            17085..17543
                     /gene="flgN"
                     /locus_tag="PLQ68_02120"
     CDS             17085..17543
                     /gene="flgN"
                     /locus_tag="PLQ68_02120"
                     /inference="COORDINATES: protein motif:HMM:NF016985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FlgN"
                     /protein_id="HXK71475.1"
                     /translation="MTNLFEEIMDLYSKQVDIISQFNEVSQQKVKILESNDTSLLNSI
                     IKYEEALLMKFSAVENKRQRTLDIVKDQYGVKKITKRIMRKHLNSNQIKQADQLSKEL
                     NTHLEEQHKLKLLNMKIVETRLHELKSALDIIEKRSNEYLEPKVLLDKKV"
     gene            17546..19006
                     /gene="flgK"
                     /locus_tag="PLQ68_02125"
     CDS             17546..19006
                     /gene="flgK"
                     /locus_tag="PLQ68_02125"
                     /inference="COORDINATES: protein motif:HMM:TIGR02492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HXK71476.1"
                     /translation="MSTFSGIEIVRKSLYANQKAIDVTGHNIANANTKGYSRQVVRFE
                     PVSDNSKSLFTGTRTSNIGRGVVLAEVKQVRFDYYDSMYRKETGIQSELSSKSSAFIY
                     INSLVRNGRDDSIPNMLTDLFNSMENLASNAQNMTLREEVKQNAILFTENLNITADSL
                     IQYKNELNEDIKTYAYEINDKSSKIVELNKMIFEFEATGKTANELRDERNLIVDELSE
                     LINITVNEKDNGEYQVMTGGYCFVDHYTVHEIEVRDNLVSPTTDGFYNQLYWKDTGTK
                     LSLTSGTVKGMLDIRDGNSQENMGIDYILTQLDNFAGSVVEQINNINRAGYTMPYASN
                     VSSTGVDFFESSNVKASNIRLSDALIESAANIAASDQEITDDTDIANNRNLMNFVALR
                     DSTDIVYEGNDIGNLEDYIESVFSKVTIATGYAKSRASSQEEIISYISNQKDSVSGVS
                     ITEETINLTAYQKAYEAAANLMKVIDEMMDTLISMV"
     gene            19017..19952
                     /locus_tag="PLQ68_02130"
     CDS             19017..19952
                     /locus_tag="PLQ68_02130"
                     /inference="COORDINATES: protein motif:HMM:NF012874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HXK71477.1"
                     /translation="MRITNGMIARRSMRDIEMAYKKLSDASYSFSTGKKNENTYDDPV
                     AANSLHRLKNDKKEITKYYANIDNARTAVSDMETALSDINRCMQKIMELSTNANNATL
                     SATAKESISQEIQQYKEQIITDFNYNSAGIYIFGGFNTNSKPISVNEGNYLYNGTDIL
                     TMTEEQHLQALGEKVMVNVSKGMTMDIRISALEIIGSGEENILNNIDALSDVIKAGEF
                     DSTSFESLNRILEQNYDKVLTNITKTGAAANRLDSVKKQLDSRESTLNDRISDLEDVD
                     TEKAILNYKLAEQAYEASLSASVSAIQLSLLDFIR"
     gene            19975..20373
                     /locus_tag="PLQ68_02135"
     CDS             19975..20373
                     /locus_tag="PLQ68_02135"
                     /inference="COORDINATES: protein motif:HMM:NF014660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliW"
                     /protein_id="HXK71478.1"
                     /translation="MEKAYPEKIVFKKDIYGFKDIKKYSLSKASENDNNPFKILKAED
                     ESVSFILLDPTVIDKSYNPIINDKIARLLGTRTAEDLAALCIVVIPNDIKRMTINLRS
                     PIIINVSNGYAIQTILEDERYSIRHKILDC"
     gene            20386..20628
                     /locus_tag="PLQ68_02140"
     CDS             20386..20628
                     /locus_tag="PLQ68_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017211306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator"
                     /protein_id="HXK71479.1"
                     /translation="MLVISRKAGESIIIGDDIEVVIIEAQNGKVKIGVKADNSIRILR
                     KEIIDEVTKTNIQASEKLGSISIDTIGSLISKEAKE"
     gene            20625..21701
                     /locus_tag="PLQ68_02145"
     CDS             20625..21701
                     /locus_tag="PLQ68_02145"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HXK71480.1"
                     /translation="MRYVPTTFLKADMVVGKDVYGRYGEVYLKRNTKLTLKYISQLEI
                     LGYLGIFIEDEISEGIEIKEIVNPRVRVEAINKVKDVYTAIKNNNKGILNNWKEIDQL
                     TDNIISNILSNEDTVYNIMYMKTHDDYTYTHCVNVALIVAIVAKKTMVSKAILMDLFK
                     SALMHDIGKLFIPLSILNKPGRLSTDEYELIKQHPLKGYDYLVEQGCLTTQGRRGVLM
                     HHEYHNGKGYPFGISDAGINKYGKILCICDVYDALVSDRPYRKAWSQSEAFEYIMANT
                     GSMFDPEIMKIFIDNITPYNPGTIIQLSNGAKAIVVKNNKGLPLRPIIRIFEEDSIKV
                     QLRDVDLKENSDYLNIVIDKEINY"
     gene            21924..23114
                     /locus_tag="PLQ68_02150"
     CDS             21924..23114
                     /locus_tag="PLQ68_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015360206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HXK71481.1"
                     /translation="MRINHNIAALNTYNQLSKNNETANSALKKLSSGLRINQAGDDPA
                     GMAISEKMRAQINGLNQANRNAQDGISLIQTAEGALNETTSILQRMRELAVQAGNDTA
                     TANDRATIQAEMDQLAQEITRIADTTKFNGEALMSSSHVFSGNLQIGADAGQTLDVQL
                     TQMDALTIGVGIGSDAPTLAAAEYTDVAGTFSADGGTIDTAGYTTNLQSLEITFDVVT
                     VGNGTDTAGTANVTVNGTSVLLTADSVAADGTYTLSHSDFSDIIASGESITIDAGAAL
                     EADMVTTITVDALQNGDLAGLDVSSGAAAAAALTSIDSAIDMVSTQRSNLGSYTNRLE
                     YTITNLETASENMTAAESRIRDVDMASEMMEYTKMNVLSQAAQSMLAQANAQPQNVLQ
                     LLNS"
     gene            23203..23577
                     /locus_tag="PLQ68_02155"
     CDS             23203..23577
                     /locus_tag="PLQ68_02155"
                     /inference="COORDINATES: protein motif:HMM:NF015602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlaG"
                     /protein_id="HXK71482.1"
                     /translation="MNDSYIDSLTGVSAVRQVESQVNKENQTVRQQTESRSDFKTFNE
                     ELQFCDNEKQLDEQLETLNNTLRNENKKVEYSIYEETNTVIFRVIDTKSEKVIKEIPS
                     TKLIDFSVAVLERFGLIADKEV"
     gene            23590..25479
                     /gene="fliD"
                     /locus_tag="PLQ68_02160"
     CDS             23590..25479
                     /gene="fliD"
                     /locus_tag="PLQ68_02160"
                     /inference="COORDINATES: protein motif:HMM:NF018852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HXK71483.1"
                     /translation="MTDQVSSLKYSSSKISGLASGLDTEAMIESLMKVERQKVIKIEQ
                     ELEYTTWQQDAYRDVIHNLSDFTKSQFNISDNSKNILSTTNLKSVNIESSEYASILAN
                     GDANIGDYTINEIVSVAKAATISSSSTVKNGIQGNKDLSLMSSIDVEGMSFYARLDGE
                     QYEISFSQNYTDTASLVSDIQSQFDNIFGAGRVTAGINVDGALQFNASGSILQIIDNI
                     ESDTKLEYIGLRSGDMSILNLRGSLSENFGYEENVSFSINGTEFNFTYEDSIENIMKT
                     VNESTANVKMTYSSITDKITISSTKTGSNAEIDIQNLSGNFFGPSGIVKIDQGITKNG
                     QDAMFYLNDESKENLIIRSSNTFTIDNVTYTLKEETVTPIDFKVENNEDGVFESIKSF
                     IEEYNSIVEELTSHVTQKRDYDYEPLSDEQKKEMSEDQIADWEEKAKQGLLKADNTIY
                     SMLNELRTAFITGVENISMTFGSIGINTSSYTNNGIITIDETVLKGAISGKFDDVVSL
                     FTRESSISYLRDLSSEDAAERFDESGFCQRVNDIVKKYITTARDKNGNKGLLVEKAGI
                     ENDASEGTNALTRKIDEIKERLDRANDLMDKKERGYWSQFSNLEVAINKLNTQSGYVS
                     SMMEQ"
     gene            25481..25873
                     /gene="fliS"
                     /locus_tag="PLQ68_02165"
     CDS             25481..25873
                     /gene="fliS"
                     /locus_tag="PLQ68_02165"
                     /inference="COORDINATES: protein motif:HMM:TIGR00208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HXK71484.1"
                     /translation="MKQENILKEYSDNNILQSTPEGLILLLYNKCISNIEAAKYYNGK
                     KNIEKTNKYLQKSQDILYYLQSTLDNKFEISKQLSTMYDFIINQLTVANIKKDSEKMS
                     IALDFTIQMRNLWVEVTEENRKYKSKAV"
     gene            complement(25953..26342)
                     /locus_tag="PLQ68_02170"
     CDS             complement(25953..26342)
                     /locus_tag="PLQ68_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HXK71485.1"
                     /translation="MEVSEAQEMYLETILFLLMNKKRVISIDVAKYMGYSKPTVSVMM
                     KQFRDEGLINMDYDSGITLTDKGLSIAQKIYEKHELLAAFFMALGVDKDTAYKDACKI
                     EHDISDSTFSLLKKHYKEIRSNKRTLF"
     gene            26622..28715
                     /locus_tag="PLQ68_02175"
     CDS             26622..28715
                     /locus_tag="PLQ68_02175"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004463374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HXK71486.1"
                     /translation="MTNNTFNDHMLEVFLFETNELMEQLEEQLLHIEDSKKMTKENID
                     EIFRIMHTVKGSSAMMQFDEISHLTHSLEDLFFYIRENGLEGIDADEITEVGFVTIDF
                     IRNEVIKLEQGKVSDGDAGSLKERIVLMVEKFKKPKNNGNKNEDLKELLKESMESDEL
                     ENISDDVIENSDTAYEEDKNKSNKKLYEAKVIFDEDCGMLNIRAFTVIRNITDYCDNL
                     SYLPEELMDSDDASDIIAKNGFIIRFSSDHEYDELKNILKESLFVSDIKLDMVSDNVE
                     EEKPNDDAKNGDIEKTDDNLAIDVSGSSHKKSIISVNIEKMDKLMDLVGEIVITESMV
                     INNPDLKGIQLDNFHKAAAQLKKLNNELQDVVMSIRMLPIAATFHKMHRIVRDMNKKL
                     SKDVELFIYGEETEVDKSIIDNLGEPLLHLVRNSMDHGIESTIDRKKAGKSEKGKIIL
                     DAQNNGSEITIRVSDDGKGLDKHKIYEKAKAHGLVAKEEEELTDKEIYSMIFLAGFST
                     NKEVTEFSGRGVGLDVVRKTIEKLGGTITVDSAINEGTAFNMRIPLTLAIIDGMKVSV
                     GKSIFTIPITSIKESMKIKNEDIVKDTSDREMVMIRGKAYPVIRLHDHYIIDTEVRDL
                     TEGILVMVESEDNAYCIFADTIIGEQQVVVKPIPAYVGKCQNANSGIAGCAILDDSNI
                     SIIIDVMGLHGQIIK"
     gene            28729..29199
                     /locus_tag="PLQ68_02180"
     CDS             28729..29199
                     /locus_tag="PLQ68_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009617834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HXK71487.1"
                     /translation="MDNDKNMIQIEEDTQKDRYLTFSIGTESFGVDIKYVTEIIGIQP
                     ITIVPEVPDYVKGIINLRGKIIPVIDIRLKFKKESIEYDDRTCIIVVDVDDVTAGFIV
                     DNVTEVITIAGDDIVPPPEYKECFQNNYIKNIGKTPNGIKLLLDCQMILSGEII"
     gene            29277..30986
                     /locus_tag="PLQ68_02185"
     CDS             29277..30986
                     /locus_tag="PLQ68_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010251103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HXK71488.1"
                     /translation="MKWYLNMKMGKKLVIAFVLVAIIAGIVGFLGYSSVQDIRTSQTY
                     IATVTMPRIENLITVREKVTAIMLGQRGLMMEKMKAQSLRDAQYDWISDARVDLGTAW
                     DNIMSLEILDSEKEQYAKGEEYVTNFLAEADKLDVMAKKKDELIQGGADYDDMAVMNL
                     DEQTYQQSLVVRAIYLEFDKLIETIIVSDKAIADETYAQAEQGADQAAVFLIVIIGVA
                     ILVSIILGVLISRMISKPIDEIVGYANNIAQGRLDDHIEVKTKDELGDLQRAFGQILE
                     SLNTTMKDINEAAEQVTTGSSQVSDGAQQLSQGSTEQASSIEEITASVTQVAEQTKDN
                     ASNANEANRVTLEVKDNASKGDKEMINMLDAMKEINDSSQNISKIIKVIDDIAFQTNL
                     LALNAAVEAARAGQHGKGFAVVAEEVRNLAARSANAAKETTTLIEGSMDKVNVGTKIA
                     QETAKALADIVKGVETATTIVSQISSASNEQAAAIEQLNEGLNQISKVVQNNSATAEE
                     SAAASEELSSQAELLKEMVNRFKLATDDSKKELRKETTVKKEVSKSVPKQIKLDDDGF
                     GKY"
     gene            31098..32654
                     /locus_tag="PLQ68_02190"
     CDS             31098..32654
                     /locus_tag="PLQ68_02190"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HXK71489.1"
                     /translation="MKKLKITGKLVMMVLPSMLVLFVVIVFSVFNNLNVYNRTYENIY
                     DQLYVNTELLLNADRDLYQALLAEQELYIEGDTLSETKKNELVADYKENYIQAQDRIV
                     SVSNNLINISALYEDFSHEGSGKNLKTLVAEFNTDFDKWVATYNPETNKGNYLTALGY
                     FDQAREKIDFASQLLDDYSKVQAEVESSNMTRNTVLFIIFGVVICAILTVIAQSVSKT
                     IKKPVMTLKKMADRLAEGDLTVNENTNSKDELGDLQRAFNKTALNLRETLAEINNSSE
                     QVAAGSTQVSDGAQELSQGSTEQASATEQIAETVGKMRLMINDNAKNAQKANEISVLM
                     GDDVITSNEEMDKMLESMKDINKSSQNISKIIKVIDDIAFQTNLLALNAAVEAAHAGQ
                     YGKGFAVVAEEVRKLAARSANAAKETTTLIEESIQKVNVGTTTAKGTAEVLEKIVVNI
                     SKTADIVKNISESSQVQATQVEQLNTAVNQISAVVQSNSATAEESAAASEELNSQVVM
                     LKEMVNRFKI"
     gene            32679..33194
                     /locus_tag="PLQ68_02195"
     CDS             32679..33194
                     /locus_tag="PLQ68_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HXK71490.1"
                     /translation="MYETESEDMLNENTSKDMYLTFRLNNEEYGFDISHVTEIVGIQP
                     ITLIPESANYIKGIINLRGKIIPVIDLRLKFNRTEKAYDERTCIIVLAYGGTDVGFIV
                     DNVSEVVTLKEDQVIPLPSYGSELRNNYVKNIGKAANGIKLLLDCSRILGKNELEMIE
                     GLLTNKSDDKN"
     gene            33181..33993
                     /locus_tag="PLQ68_02200"
     CDS             33181..33993
                     /locus_tag="PLQ68_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008423652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HXK71491.1"
                     /translation="MIKISDNEFRRLVTFVKKNYGINLSQKRNLIEGRLGNIITGKGY
                     SEFSDYLDYVFSEKGTDEMVNMVNKLTTNHTFFMRESSHFDFFRQTVLPYLKNSKNNY
                     DLRLWSAGCSSGEEAYTLAMIIDDFFSGSTEPWEKTMLATDISTIMLNKAEDGIYEQS
                     SVLKMPKSFQTKYFQPYGNGQFKVVQSIRQKIVFKKFNLMTEIFPFKKKFDVIFCRNV
                     MIYFDKETKDALLKKFFNSIEDGGYLFIGQAESITRSSTGFTYVMPSIYRKV"
     gene            33997..35025
                     /locus_tag="PLQ68_02205"
     CDS             33997..35025
                     /locus_tag="PLQ68_02205"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017212172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HXK71492.1"
                     /translation="MVNDIKVLIVDDSMFFREYLSEGLKKQNGIEVVGFAKDPYEARD
                     LILELKPDVITLDVNMPRMSGIDFLKKLIPQYPIPVVVISSASTNIFDALDAGAVDFL
                     KKPDIKDKDGTVFFKELAIKIKISSQVKYINVRKDEKSNFSKGSTDIHDVDKYIIAMG
                     ASTGGTEALFDVIKRLPVNLPGIVIVQHMPPVFTKMYADRLNNQTDFEVKEAENGDLV
                     KKGRIIIAQGGIQMLVVKRAGEYVIKLSGTEKRSGHCPSVDVLFSSVAEAAREKSVGV
                     LLTGMGVDGATGLLEMKKKGAYTIGQDEATSIVYGMPKAAFSIGAVMAQLPLLDIPGK
                     ITNYFNKI"
     gene            35035..35649
                     /locus_tag="PLQ68_02210"
     CDS             35035..35649
                     /locus_tag="PLQ68_02210"
                     /inference="COORDINATES: protein motif:HMM:NF016398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheC"
                     /protein_id="HXK71493.1"
                     /translation="MTNIDSFNEYQVDVLKEIGNIGSGNAATSLSIMLRGRISIDVPD
                     VRIIDVDGISNLLGGPENQVAGVLVLMKGDIEGMLLFLLEKSAVSHLLKGLLNEDLKS
                     LDSISEMGISALLEISNILSGSYINALSQLLNYSISLEAPRIAVDMVGAILNYPASVF
                     GMMKDELMLIEESFYSGDKSFKSNLLIMPEPASLDRMFEKLGAL"
     gene            35646..36119
                     /locus_tag="PLQ68_02215"
     CDS             35646..36119
                     /locus_tag="PLQ68_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012424081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HXK71494.1"
                     /translation="MTGKQIVVGISDLNTAYSPDELISYALGSCIGICLYDKFQKIAG
                     LSHILLPDSSTYKAVDNMKFADTAIKELISRMIRLGCRKNNLTAKIAGGANMFSWSGE
                     TVGDKNIKSVEEQLRIHGIPLLAKDVGGDYGRTVSICAETGVVTVKALTRGVKTL"
     gene            complement(36121..36540)
                     /locus_tag="PLQ68_02220"
     CDS             complement(36121..36540)
                     /locus_tag="PLQ68_02220"
                     /inference="COORDINATES: protein motif:HMM:NF024312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK71495.1"
                     /translation="MKAKNSTDVRKNWSETIDSAVRSHPEFIKRNRDLLTLINTSHLE
                     KLLTKYNIYVEIEYVDNAVYIAKIPIFDIFVKADTISEVSDLSVSRMIEFCNKYYENL
                     DINVNLPDRADLLPYVLKVIIAIINEDDIKDMLIIKN"
     gene            complement(36715..37074)
                     /locus_tag="PLQ68_02225"
     CDS             complement(36715..37074)
                     /locus_tag="PLQ68_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006692844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXK71496.1"
                     /translation="MTEGVLVVDDAAFMRMMIKDILSRNNIPLAGEAANGMLAVEKYQ
                     ELQPKLVVMDITMPEMSGVDALKAIKKMDPKANIIMCSALGQQDMVMESIQIGAKDFI
                     VKPFQEAKLVQAIKKFV"
     gene            complement(37087..38115)
                     /gene="fliY"
                     /locus_tag="PLQ68_02230"
     CDS             complement(37087..38115)
                     /gene="fliY"
                     /locus_tag="PLQ68_02230"
                     /inference="COORDINATES: protein motif:HMM:NF005995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch phosphatase FliY"
                     /protein_id="HXK71497.1"
                     /translation="MGKELTQAEIDKLINGGSPSPLTEEDIDMLGEIGNIGMGNSATA
                     LSKILNRKVSITTPKVVILEDNNMPTEYAIPYVTVEITYIEGISGKNVLNLRDEDVKI
                     ITDIMMGGKGEVKEGPIDEMNLSAAGELMNQMIGASSTALSEMMNTRLNISTPDVKIT
                     DYSTENIDDFIKGETLVMTIFDLDVEDTLKSEIMLVMTLDFAKKMIEKVKQQYTESLS
                     TPVQSEQEVKASKSNTAEPPSKVNIQKVELQTFGEDNKKPNMFGNRYDILMDVPLEVS
                     IEIGRVKKPISEILKFTSGTIIELDKLVGEDVNIVINNKTIGKGEVVVIDEYYGMRIS
                     EILDKNSI"
     gene            complement(38117..39091)
                     /gene="fliM"
                     /locus_tag="PLQ68_02235"
     CDS             complement(38117..39091)
                     /gene="fliM"
                     /locus_tag="PLQ68_02235"
                     /inference="COORDINATES: protein motif:HMM:TIGR01397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HXK71498.1"
                     /translation="MKDILSQAEIDELIQSLNTKSEEPVLVKKEKVKPYDFSRPNKFT
                     REQVRAIEDIFSNFAKLLSLRVSDFVRTTCFFEVTFIEELTFFEYTNSVQKSSLVGIF
                     RVNQSDHNASVELSYSAVESIINLILGGDGNCNQNIFSGYTEIEINLIKRVLNIFIQP
                     MIDVWKGFADIKLNLQRVENMGQHIQIAAPSDIIAIVTLKAHIAENEGFINFCIPYFL
                     IEPVLRNIAVTKGFTRTSKPDGISHEEFLKNKITNNIVEMICNLGKAYLTLDELERIQ
                     AGDVIKLKKKTTEQAELLLNNIPKFSVDLGQKNNKIALRINNTIERTS"
     gene            complement(39100..39543)
                     /locus_tag="PLQ68_02240"
     CDS             complement(39100..39543)
                     /locus_tag="PLQ68_02240"
                     /inference="COORDINATES: protein motif:HMM:NF015693.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HXK71499.1"
                     /translation="MKNVIIIVATVFLTVALTLVILIAFPDTNAYKPEEPSLTASPTE
                     PIEEVIYTYVIEDTFITNMKNSNGFVQASFMLEITDQTDLEYIERHNYILTDTIINVL
                     RNTTEEEYLKDDIQQMLRERLKYVLMTALGIDSIQNVFFLELIIQ"
     gene            complement(39540..40373)
                     /locus_tag="PLQ68_02245"
     CDS             complement(39540..40373)
                     /locus_tag="PLQ68_02245"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HXK71500.1"
                     /translation="MKRKKPDPPEGAADWIVSYGDVMSLLLTFFVMLFSFSTIDAVKW
                     QQIVESLRGRMGVSIHTEQQPFNPIKKDKDSSQNKSNLIDSIVERGNPIPLVNSDTEI
                     PSDPEDTKIPPEEIGNTIDEDFGKLYGELMRLITYKSGIWDVDIIRNEESIILRFGNK
                     ILFESGIADLDTEAADIIYEIVGVIAEYESVLDKLVIEGNTDNIPINTARYRDNFELS
                     IYRALNVFYFIQQEDLFPAKKIEIKGYGENNPIASNDTEEGRALNRRTDIVIMKAEVK
                     E"
     gene            complement(40354..41127)
                     /locus_tag="PLQ68_02250"
     CDS             complement(40354..41127)
                     /locus_tag="PLQ68_02250"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HXK71501.1"
                     /translation="MSVLGIALAGFLVIFAILENGQLSAFYDKASILIVIGGTIGAMV
                     CNYPLKNLLSSVKAFIKVIKFKPQNRKDILDKLVELAYASKKEGLLALEKYTDEMKDE
                     FFKKSILLIVDGTSPEDARNTLESDIAITEQQDLLIQDIYTQGGKLAPAFGMIGTLIG
                     LINMLGKLEDTGTLAASMSVALITTLYGALLANIFFIPVSGRLKFISNNEVECKTMIL
                     EGVLSIQAGESPYLIKEKLGSYIVKSGKEKSTSSEEEEA"
     gene            complement(41127..41324)
                     /locus_tag="PLQ68_02255"
     CDS             complement(41127..41324)
                     /locus_tag="PLQ68_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004622001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar FlbD family protein"
                     /protein_id="HXK71502.1"
                     /translation="MILLTQLNDKAIVLNCDLIIHIEESPHTVIKMVNADKYIVKESA
                     LQVVEKVTMYKAEIQIRAKVG"
     gene            complement(41343..42614)
                     /gene="flgF"
                     /locus_tag="PLQ68_02260"
     CDS             complement(41343..42614)
                     /gene="flgF"
                     /locus_tag="PLQ68_02260"
                     /inference="COORDINATES: protein motif:HMM:TIGR03506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HXK71503.1"
                     /translation="MLRSMYSGVSGLLSHQKKMDVIGNNIANVNTTAFKSSNVVFEEV
                     FSQLVATATQPTEEGRGGTNPQQIGLGVSLGNITTNMSKGSIQATDNPNDLAIDGEGF
                     FVVTDGTGSYFTRAGSFNVDKDGNLVNGGGYKVMGWNRNPATGNIDISKPTEPIDFSN
                     IKMSAKSSTKISITGNLSSETLEYDSVNDENGVLHNLTIYDSLGESHTLTFKFMRTSA
                     ASDYTYELMESDDEMEITAGATGGSISFTSSGKLDTGASSIPEVQITFSNGAEGITFP
                     TGSISFDAIGFTQYSNSTSIKSNQDGYSSGELETLSISSDGQVIGQFTNGLQEHISTL
                     AVASFINPNGLQKVGGNMFTTTWNSGDANIGMASTGDRGSVATSSLEMSNVDLATEFT
                     EMIVTQRGFQANSRIISVSDSLLEELVNLKR"
     gene            complement(42661..43056)
                     /locus_tag="PLQ68_02265"
     CDS             complement(42661..43056)
                     /locus_tag="PLQ68_02265"
                     /inference="COORDINATES: protein motif:HMM:NF015896.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook capping FlgD N-terminal
                     domain-containing protein"
                     /protein_id="HXK71504.1"
                     /translation="MNIVPIDETTKYVNENKAKEAGTMNQEDFLQVLVTQMQNQNPMD
                     PMDDFEYMTQITQFSMLESINGLSMKIDNMKALDYIGKNVIASIMNEDNPTMDYIEGL
                     VESITLQDDEVLLNTQFGSVYMDNVVYVY"
     gene            complement(43075..44535)
                     /locus_tag="PLQ68_02270"
     CDS             complement(43075..44535)
                     /locus_tag="PLQ68_02270"
                     /inference="COORDINATES: protein motif:HMM:NF014207.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HXK71505.1"
                     /translation="MVIINNNEIETDKLNSVCKNNQNNAKIEDFQGILELFNAVFSKS
                     AESDSNMLLKDDNIHGKLLECMEISDSEEDTFEKIEYLDLLLFVQQNHIPMDKFIENY
                     SSIFKAYSEKIPDLNNSSFINTINKDIVNFNIENDNNPVSYEYLNNRFSLKNLSYVKN
                     SDIPLKNTEIEKNNSTIENVSKGIVSHSYSNKEINTGFEQTPIKPLTIESDLLNENKS
                     INMQLNEFIDNFESQELVNYDKFNTEKKSILLNSDSNISLKQNSLEKMENINVISKID
                     AENANLAEGFTDSDTSNEPGKPKNKPEYVPFKSEENTVSADNIFSKGEIRTVNINQNS
                     TNPIQKDQLLNQIVDKIDLSCKNGITNIEIHLTPEIFGGMEIETTFDGEQIKLNLISQ
                     KDEVSKILISETEKLTAMLTEKGYKVEQIKVFEKSANDMSGYGGTGYSFTNNNHGNTE
                     NPHYKLKPVHVINNEAVNENETGKIISGSELVNIYI"
     gene            complement(44549..45313)
                     /locus_tag="PLQ68_02275"
     CDS             complement(44549..45313)
                     /locus_tag="PLQ68_02275"
                     /inference="COORDINATES: protein motif:HMM:NF015413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK71506.1"
                     /translation="MKKKKDDVDLIENDSVNNEDKPSKKSRIPLFKKKNKDTDNDEVP
                     DIQDKDNDDKDTSKKKIKIPVKQIIIILGISVVASVLLIFIFDWTGWRTAFRDDITTK
                     LLGKEKEVLIAQYEFEYSQKLKKAESEILKEEREMLVVEYEELEAKKTDYEERMSRLE
                     NAESLLEQTRADLIKREQELAEQIEQFEKGIIEVEELGKIYQAMEPKNAAKILGAIQD
                     TQQIVKILKTMKTDSVAAILELMETAKAADILSKLN"
     gene            complement(45318..45752)
                     /locus_tag="PLQ68_02280"
     CDS             complement(45318..45752)
                     /locus_tag="PLQ68_02280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK71507.1"
                     /translation="MKAFKFSLQAVLDNANNIKDECSLRLKEALDNLRAVEHLKDLHI
                     KMLKDNEAKLLNDCNSITFREIKIYMEELAVKIEQMNKEIEKLTTIKDQRLEELKKAK
                     INCSAYEKIREKKYSLYLDEIRKFEEKQIEELINYNYIAKAE"
     gene            complement(45764..47080)
                     /gene="fliI"
                     /locus_tag="PLQ68_02285"
     CDS             complement(45764..47080)
                     /gene="fliI"
                     /locus_tag="PLQ68_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012159338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HXK71508.1"
                     /translation="MEKINLNKYFDNVENTFCYRTYGEVKKIIGSSIESSGPRAEIGE
                     ICMICKKNDDINNSILAEVVGFNEKKILLMPFSDIKGINNGDRVYATGQQLKVKVSDK
                     LVGKVLDGLGRPVDSSDITDYEYRDAYADSPNPFNRKRISEPLPLGIKAIDSLITCGK
                     GQRLGIFSGTGVGKSTLLGMIARNSSADINVIALVGERGREVNDFIEKDLGKEGLKKS
                     IVLVATSDQPAIIRIKCALAATAIAEYFRDKNKNVLLMMDSLTRFAMAQREVGLAAGE
                     PPVARGYTPSVLSVLPRLLERTGYNDQSCITAIYTVLVEGDEMNEPISDTVRGILDGH
                     IVLSRAIASKNHYPAIDVLNSISRVMPDIIDKKHYNAAMNTRNLMATYENYRDLISIG
                     AYKEGSNKEIDQAIKNIDNINGFLRQDTKETALYEDTISSLETLII"
     gene            complement(47081..47797)
                     /locus_tag="PLQ68_02290"
     CDS             complement(47081..47797)
                     /locus_tag="PLQ68_02290"
                     /inference="COORDINATES: protein motif:HMM:NF014196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliH/SctL family protein"
                     /protein_id="HXK71509.1"
                     /translation="MSDLYKSSDVVLMNSKYYISFTESKPEAISLNSFEEHQLNDSDD
                     NESYQIADQIIRNAEQEAAKILSEAKIQADNILKQAKTQKEEQFSNGFAQGIEEAKKQ
                     YAEQLNNLNDALNNTLTEKKEIIDNTESQLIDLAVNIAQKILHEKISLSEENIKKYFE
                     NAIEDILRNDFEVICVRLNKYNIEKFYPDWEVEKYMLNIKHDARLDNSSCIVETETGD
                     IDISVFTQLNEVSRKLVNVK"
     gene            complement(47856..48866)
                     /gene="fliG"
                     /locus_tag="PLQ68_02295"
     CDS             complement(47856..48866)
                     /gene="fliG"
                     /locus_tag="PLQ68_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009433 - bacterial-type flagellum basal
                     body, C ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HXK71510.1"
                     /translation="MNSTYKISGKEKAAYLLIMLGKGQASKIMKYFNEDEIKTITFEI
                     VNHNTIDKEERMMILQDFYNSCLAKSYIDKGGVDYAREVLYEAVGPQKAMELITDMMD
                     LEKSKPFDFMKDIDPVEVFNFIQYENDQTIAFILSYLKNSQAASVLAMLPRERQANIS
                     VKIALMDKISPDIIKDIEKVMGEKFTRSGIQTYTKTEGVEVLVNILNAVDRSTEKQIF
                     EMLEEENDNLANEVKKRMFVFEDIINLGNKEIQRFLSEVDTKDLSVALKTATEELKEL
                     ILRNLSKRAKQMVTEEMNLLGPVRLSEVEKAQQALVSVIRALDEKGEILIRKTGDDKI
                     ID"
     gene            complement(48881..50392)
                     /gene="fliF"
                     /locus_tag="PLQ68_02300"
     CDS             complement(48881..50392)
                     /gene="fliF"
                     /locus_tag="PLQ68_02300"
                     /inference="COORDINATES: protein motif:HMM:TIGR00206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HXK71511.1"
                     /translation="MSESIKSILSKIKGFWSGLEKKKKSSIILMGILFAIGLSVLLIL
                     FTRTTYTVMYTGLDVYEAGEIYTKLTDLGADVKLKNNTTILINETQEDKFRMTLATEG
                     YPRTGLSYDIYTDNVSMGISESEKQTYILFQLQDRLQNTIKTLKGVNGAVVTISKPDE
                     TLFVLESQKKPITAAVVIDVASGYSLSSANVKAIEHLVAKSISGLSNENITIIDTQMN
                     VLNRKSDSEIGTASEKYELQKSLENEIENKVISIIEPVFGFGNVKVAASIKMDYSKIS
                     EQKTEFTPVNDEDGIPYSITEKKETVIDSQSATLDNDNDTILGSEATSSETVTQLYVN
                     QNTTSIEHENGVIDDIKLSIMINSPGVTDDMLTNIQKMAANAVGGSETDIIVQAVEFA
                     ISNDVDDALSNAVVNTANVSFLLDPDFILKSVMIITMFVLGLMIITILKKGLAKTKNQ
                     TASTTRAKYESLDLEQLGQKKNPEDDYRSEIEKYVDKNPEGVADILKKWISED"
     gene            complement(50404..50715)
                     /locus_tag="PLQ68_02305"
     CDS             complement(50404..50715)
                     /locus_tag="PLQ68_02305"
                     /inference="COORDINATES: protein motif:HMM:NF014143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HXK71512.1"
                     /translation="MAIQSISGISQIQNIQQLTDISKNNLNQTSKTQFNEVFDNLIKN
                     MVEAEINTENSLNMLASGDIASISKAIVGLAETDLTIKFTMEVRNKLVDAYKEMMQMQ
                     V"
     gene            complement(50746..51174)
                     /gene="flgC"
                     /locus_tag="PLQ68_02310"
     CDS             complement(50746..51174)
                     /gene="flgC"
                     /locus_tag="PLQ68_02310"
                     /inference="COORDINATES: protein motif:HMM:TIGR01395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HXK71513.1"
                     /translation="MANIAAFNISSQALSVYQTKMDLIAQNIANADTIQTDTNEPYQR
                     READLVQKKLFGAVFEDVKENKEVLSVAISSIKEDETPFKLVYDPTNEFADENGYVTK
                     SNVDVTKEMMDLLSASRAYEANLVAFNTTKSMITKATDIL"
     gene            complement(51180..51524)
                     /locus_tag="PLQ68_02315"
     CDS             complement(51180..51524)
                     /locus_tag="PLQ68_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003154260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body protein"
                     /protein_id="HXK71514.1"
                     /translation="MYVNLLKNTQVITNGMDKCLQRNQVLSSNIANSETPGYKAKDVF
                     LNILEPVTPEGVIRYSITERTDVEAKENGNTVDIGKEMTSIVENVLEYNALVNALSKS
                     IKGMEYAIEDRR"
     gene            complement(51886..>52683)
                     /locus_tag="PLQ68_02320"
     CDS             complement(51886..>52683)
                     /locus_tag="PLQ68_02320"
                     /inference="COORDINATES: protein motif:HMM:NF037167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK71515.1"
                     /translation="LNKQAKSLYDLKKSNYLTNKNFFEKFLERLGKPFDDYLEINIKN
                     SEFYTINYLDNHIGFFSILEEKYLTSFYIPDSLLSHGQEIFKLILSMFDVELMLVTSF
                     DEISLCLVMDYVSKIKTHYIYFKLNSLPVRKAEYPSKNFRKAEIEDISSIKKYTQNFI
                     DGHEERILKNQLFILEDDNKSFLGLAVMIPHSIQKGICSFGIYVREESRNISVGRSML
                     LHITKEAEKMNYTPRCACPLSNDIYRKTLESGGFVSESRLFIAYLRQ"
BASE COUNT        17848 a         8338 c         9815 g        16682 t
ORIGIN      
        1 gatattatca tatttaaaag attaatcata tgtgctacaa tgtagtttgg taacaacgag
       61 gttattaatt tatatacatt ctaaaaggag aaacaaatgg gtaaaaaact taaaacgatg
      121 gatgggaatc aagctgcggc atatgtatct tatgctttta cagatgtagc tgctatatat
      181 cccataaccc cttcatcacc aatggctgaa aatgtcgatg aatgggctgc tcatggtcaa
      241 aagaacatgt tcggtcaaga ggttagagtt gtagaattac agtccgaagc cggtgctgct
      301 ggtacagtac acggatcatt acaggcagga gcacttacaa ctacttatac agcatcacag
      361 ggattattac tgatgattcc taatatgtat aaaatagctg gagaacttct tccatcagta
      421 ttccacgtta gcgccagagc tgttgcaggt catgcgcttt caatattcgg agatcattca
      481 gatgtatacg ctacaaggca aacaggattt gcaatgttag cagcaggcag tattcaggaa
      541 gtaatggatc ttgcaggagt agctcacctt gcaacaatta agagcagagt tccttttgtt
      601 catttctttg acggattcag aacgagtcat gaacttcaga aagttgaagt aatagactat
      661 gctgattttg ataagcttgt agataaggaa gcaattaagg attttcgtaa cagggcatta
      721 aatccttcca gcccagttct aagaggaaca gcacagaatc ctgatgtata tttccaggca
      781 aaggaagcat caaacaggtt ttatgatgca gtacctgcaa tagtagaaga ttatatgaag
      841 gaaatttcca aagttactgg cagaacatat aatctgtttg attatcacgg agccaaggat
      901 gctgacagag ttataattgc tatgggcagt gtaacagata cagcagaaga agttgttgat
      961 tatcttgtag agaaaggcga aaaagtagga ttgctgaaag taagacttta cagaccattc
     1021 tccatcaaac atttccttaa cgcattgcca aagacagtta agagaatagc tgttcttgac
     1081 agggcaaaag aaccaggagc aacaggcgaa ccattgtatc ttgatgttgt ttcggcatac
     1141 aaaggagaca aagaagctcc aattatcgta ggaggaagat atggattagg atcgaaagat
     1201 actgttcctt cacagatagc atctgtctat gcaaatcttg cacttaacga gccaaagaat
     1261 aatttcacaa taggaattaa cgatgatgtt acatatttat cccttcctgt tgccaagaaa
     1321 ataaatacta ccccaaagag tacagtaagc tgtaaattct ggggactcgg ttctgacgga
     1381 acagtaggcg ctaataagaa tgcaatcaag ataatagggg accatactga tatgtatgct
     1441 caggcatatt ttgcatacga ctccaaaaaa tccggtggta ttacagtttc acacttaaga
     1501 tttggaaaaa gtccaatcaa atcaacatat cttgtatcag atgcagattt cgtagcatgt
     1561 cataatcaga gttatgtaga taagtatgat atagtatcag acgtaaaaaa aggtggaact
     1621 ttccttctca atacaggatg gacagtcgag gaaatggaag aacgcctgcc aggccagatg
     1681 aagagatata ttgcaaataa tgatgttaag ttctacacaa tagatgctgt taagatagct
     1741 caggaactag gtcttggcgg aagaactaat atgatactta tgtctgcatt ctttaagatt
     1801 tccaatatta tacctattga tgatgcagtt aagtatatga aagacgctgt tcttaaatca
     1861 tatggcaaaa aaggtgaaaa agttgttaac atgaacaatg ccgctgttga taagggaata
     1921 gaaaacatag ttgaagtaaa agttccagcc acatggaaag aagcagtaga ccttccaaaa
     1981 gaagataaaa aataccctga attcataact aatgtagtag aggttatgaa tgctcagcaa
     2041 ggtgattcac tgcctgtatc gacatttgtt ggaattgaag atggaacatg gcagcaaggg
     2101 acagcaagat acgaaaaaag aggtattgca gttgaagttc ctgaatggaa catagataac
     2161 tgtatacagt gtaatcagtg ttcatatgta tgtcctcatg cagctataag accgttcttg
     2221 gttaatgaag aagagcagaa gaacgctcca gaaggttttg aaactaaaaa agctgttgga
     2281 aaaggattgg atggaattac attcagaatt caagtatctc catatgactg tgcaggttgt
     2341 ggaaactgcg cacaagtttg tccttcaaag gaaaaagcac ttgttatgaa gcctattgat
     2401 actcagatga atcaggcagt taactgggaa tatgcaacga aactttctgt aaaagaaaca
     2461 cctttcaata aatacagcgt aaaaggtagc caattcaata tgccttatct tgaattctca
     2521 ggagcatgcc caggttgtgg agaaactcct tatatgaagc ttatcactca gttattcggc
     2581 gacaggatga tgatagcaaa tgctacagga tgcagctcta tctggggagg ttcagcacca
     2641 gctacacctt actgtatgga ttgcaatgga aatggacctg catgggccaa ctcattgttt
     2701 gaagataatg ctgaattcgg attcggtatg tatatagcta caaggcatat gcgtgaaaga
     2761 atagaagaat tattaaagca gcttttggag tctgatgcat gtgattgtgt taaagaagca
     2821 gctcagaatt ggttagataa caaaaataat ggtgaaacta ccagaaaagc cagcgaacag
     2881 ttattagaaa aactcggatg ctgtgaaaaa aatgacatag tcaatgaatt acttgacaag
     2941 aaaaactact tcgtaaaaac atcacagtgg atctcaggtg gagacggatg ggcttatgat
     3001 ataggttatg gcggattaga ccatgttctt gcttcaggtg aggatgtaaa cgtaattgtt
     3061 tacgacacgg aagtttactc aaatacaggc ggacaggcaa gtaaatctac tccaacagca
     3121 gcagtagcga agtttgcagc agcaggtaaa aaggtcaaga agaaagacct tggtatgatg
     3181 gcaatgtcat atggatatgt gtatgttgca caagtagcta tgggagcaga tcagaaccag
     3241 tttataaagg ctgttaaaga agctgaagca tatgatggac cttccctcat aatcgcatat
     3301 gcaccatgta taaatcatgg tattagaaca ggaatgggaa ccacacagct tcaggaaaag
     3361 aaagctgtcg aagctggtta ctggcatctt tacagataca atccaatgct agcagaagaa
     3421 ggaaagaacc catttgtatt agattctaaa gagccaaccg gcagtttcaa agatttccta
     3481 gcaagtgaaa caagatatac atcacttaag gtaaccttcc ctgaattagc tgacgattta
     3541 tttgaaaaga cagaaaaaga tgcaaaactc aggtatcaga catataagaa gctgtcagag
     3601 caggagtaat tacaagtatt taaaagttag ttaaatttta tatcttaagg agtcatgtat
     3661 aatacatgac tcctttttta tgatataata ccgatattga gcattctgag taattgcagc
     3721 aaacggggga acatgttgaa tctaagaatt actgactcag atatcctaaa cgaaggtgaa
     3781 ccgcaatatg tatcacgaat ttctaaatat ggttcaaggg gagttttgtt gaataataaa
     3841 gggcagatag ctctgatgta tgtatcagat tcagatatat acaaacttcc aggcggtgga
     3901 atcgaggaag acgagacaga agagcaggca tttataagag aaataaagga agagacagga
     3961 taccactgtc atattttatc ttatcttgga attgttgaag aacataaagc taagaaagat
     4021 tttcttcata tatcacattg ttactatgca gagactattg gaaaaaaagg ttctctcaag
     4081 ttaacaaaag aagaaaagaa gagagggtat aagccacatt ggtttgattt aaatttggct
     4141 attgagattt tggaagaatg ttttagaaag agtatcggtt acagaacttt atttgtttta
     4201 aaaagagatc ttgcaatatt atattatgta aaaggactaa caaaaaatga ataatataac
     4261 cttaaatgga aaatggaatc tttactttac agaagagaaa gatgcttaca tagataaacc
     4321 ttcacaactg gaaaatagca attacagatg catagaagcg gaagttccgg gtaatgtgga
     4381 agctgatctt gtaaggacaa gtattgaaaa agacccatat gtcaaccaga attcctttga
     4441 ttatatgaaa tacgagtatt atcagtggtg gtatacaaga acagttgata taccgaacaa
     4501 tttaaatggt gacaaatata ttcttctttt taacgggata gatacaattg ctgatatata
     4561 tcttgatgac atattaattg gtcatacaga aaatatgctg gttgaacatg aatttgatgt
     4621 tactaacctt ataactccca gcaatacata taccctttca gtccggatat attctgttat
     4681 gaactacata agacgcaaag aatattctgt atggatgaga ggatgtcgcc actgctcgga
     4741 acttccatat atacgtaaag ctccacatat gatggggtgg gatatacttc caaggctggt
     4801 atcagcagga atatggaaag atgtaaaaat aatagcagta aagaatacca ggataacgca
     4861 ggcatattat gcaacacctg ttttcagcat ggataagata agaatgcgtt ttgcctacag
     4921 atttacgact gaccatgacg atctttcggg atttatgata agagtcaaag ggatatgtga
     4981 agattctgaa tttgaatata caattccatc atggtttgta agcgggaatg atggattttt
     5041 gatagataaa ccgaaattat ggtggccaag ggggtatgga gaacagaatc tttacaccgt
     5101 tacaatggag ttgctttata ataacaatgt agttgataca agaacggata taatcggatt
     5161 aagaacattc agattagaaa gaagatttga aaaaggtaat caggaattta agatttttgt
     5221 taatgaaact cctattttca taaatggaac caatcacata gctttggata tattgcacag
     5281 caaagaccat ttaagacaaa gaaaagaaat agagcttgct gctgaatgta actgcaatat
     5341 gataagatgc tggggaggca atgtatatcc tgaaaccgat ttctataatc tttgtgacca
     5401 gtacggaata ctggtatggc aggactttac tttcggtaat tcaaactacc ctcagacaga
     5461 agaattcttt aatacagcat atgaagaagc agaaaaagtt ataaagaagt ttagaaatca
     5521 tgctagcctg gtattatggt gcggggataa tgaaatagac acaacattag atggatactg
     5581 gtacccagat tacaagagca agcacaacag gatatcttca gaagtactgg aaaaagccgt
     5641 acagcagcat gacccattta gaatatactt aaaatcatct cctgaaatac ctgatggatt
     5701 tgattcatat aatgtgcctg aacagcacat atggggacca agagcatatt ttaaggatga
     5761 cttttacaag catgtttcag caaaattcat agcagaagca ggctatcatg gatgtccggg
     5821 ccttgaaagt ttaaaaaaat acataccgaa agaagactta tggccaattg aaggaaataa
     5881 gacttgggca catcacagca ctgaagatta tctgattgag gatattattg gaagaagaaa
     5941 tacattaatg gcaaatcagg taagagtcct ggtagggaag cttccagata ctcttgaaac
     6001 atttataact gtgtctcagg tttcacaagc tgaagcattt aagtttttta ttgaaagaac
     6061 aagaataaaa aaatgggaca tgacaggaat cttatggtgg aaccttcttg atggttggcc
     6121 ggggatatca gacgcagttg tggattacta ttttaacaag aagcttgcat ttgatgtcat
     6181 aaggagagta caggagcctg tgtgtgtaat gcttgatgag ataaaaggat gggaaagagc
     6241 agtgtatatt gcaaatgaca caaactctga taaatcagtt aattacaaag tgtatgaata
     6301 tcaagataat ttggtagttg ttgaaggaga tacatttatt gaaagaggga aaaacaagaa
     6361 gataaacgag ttttttgaaa ttcccggaac aaaaaaacta tatatcatag aatggacagt
     6421 agataacaaa atatacaaaa accattacat ggcagggtat cccagttttg acactgatac
     6481 tatattaaaa tggtataagg ttataaaaga actggattaa tgttgtttgg tttcattatt
     6541 caatgtaaga tatttattaa ttatagcatt gttgaggtgt tttatggata acatggaaaa
     6601 gaaaatcaga gttgggttgt taggtatagg cggaagagca agacactttg cagaccttgt
     6661 aagacagtat ccgaaaacag aactggctgc agtatgtgat ataagtgaag aagctatggc
     6721 acggtctgtc gaagaaaaaa accttcatga tataccacag tatacagatt atatgacgat
     6781 gcttaatgaa gagagccttg atgttgtatt tgtatgcaca ccaatcgaat atcattgtga
     6841 acacactatt atggcattgg aaaaaggggt gcatgtagta ggagaagttc cagtagcttt
     6901 taatattgaa caagctaaac agatggttca ggcagttaga aaatcaaaag ccaaatactt
     6961 cttcggggag aatatgtgtt ttgcaaagca tgtccttatt gtcaggcaaa tggtactggc
     7021 aggtctctta ggtgagctgc attatgctga aggagaatat ctgcacgatc tcaagaaact
     7081 tgcagaattt acaccatgga gattcgatac tctttttgga accaatggtg tgacatatgg
     7141 aactcacagt ttggggccga tattaagctg gtttgactgg gacagggtca agaaggtaat
     7201 gtgctctgga agtggaagca ggttcagata tgcagatggc agaccttttg gaatggaaag
     7261 caccactgta atgctttgta agactgaaaa agacagactt attaaaataa agaatgatct
     7321 ttccagtcca agaccttcaa tcaatcagta tgtattatca ggaactaagg gagcattcga
     7381 atcaagaagg ggaattcctg attccaaaga ttatatctgg atacaagata tagttggttc
     7441 ttctgaacaa agaccaagaa gttttcatga taattcggac acatggcttc ccttaagcat
     7501 atttgaatcc agatattttc ctcagattta caaggaatgg gtatggtatg caaagactta
     7561 tggaggtcat gaaggagcag acgcatttat gtttataagt gttgtagatt ccataattaa
     7621 caataaagaa ccatatattg atatacacag gggactggat atatccctgc ctggtatgat
     7681 gagtacaata tccattcaga ataacagtga atgggtagat gtacctgatt ccagattgtg
     7741 gtaattaaat atccacatgt atcttacgag tcagaatagc ttttctttta atgccaaatt
     7801 catctggtac aaagaaatct gaatatacga ccattgctgt attttcattt aatgcaatca
     7861 tgtcagaata tgagcatgtg cctcttctag atgtctggtt gtatatactt ataggggtgt
     7921 ttctttcaca tgttcggtca tttgaggtaa ttaattcaac tggagcatcc cacgaaagac
     7981 ctgtcgggtc agctgttgct ctgatataaa aacctggtct tccgtatgaa gctaatgtta
     8041 cattgcaagg tagtacacat aagcatggcc atacaccttt atcgtcaaac actactggtt
     8101 ttgaccaggt tcttccttta tctgttgacc ttgcaagaaa actgggggtg tctgagcctg
     8161 ttctcataag agtaatgcat gaaccgtcag gtaaaaatgc tatgtcagat tctgcaaatc
     8221 cttcagtgac aaaagagttc ttgaaaattc ttgtatctgg aatatatgga atccagcttg
     8281 cctggtacag gtaattgtaa ttttgtgaaa aagtatagta atgtgtattg taatatggag
     8341 aaaagcctaa attgtcagga tttcttgaac gtctgtatgt ggtctgccag acagtattct
     8401 catggtcgat acgaattctt ccaaaaggaa aaggaggaac taatgcatcg tatgaacttg
     8461 taatacataa tcctttttta tcataatcga aatatttgta ttcaggaaca tcttgacctt
     8521 cacttaatac aagataacgc catctcttat ccagcatttc atcagaaact ccttcagcgg
     8581 gatacaattt tattgtatcg tgttgaaaag gcatcggttc aggaagtttt gatgtatcta
     8641 ctttaacagg agtccttgtt tccatggtgt atctgtcttt gtttggaagt ttaataccaa
     8701 agcgagtttt agtattatga acatcctttg aatcaacttc ttcccatgtt tttcctttat
     8761 cttttgaaat atatagacat ggttctgttc catatgcttc aataagatcc tttgctccat
     8821 gaaatgtaac gccgatatta ccgttgtcat cagtattgat tgctggaaac tggtgtgttc
     8881 cccagccagc ttcttctact gtaggtccta atgaaacgat tacaggctca tctagtgtta
     8941 atttaacttt cacttttctc tccttgtaaa aatcagtaat gcgtaaattt aactgacaaa
     9001 ttttcttttt aagtatataa aatgggaaat ttaatgtcaa acgttttggg caattgttta
     9061 ttaggtaaac taattaacta tttaattagg acataatctc aattaattgc taaattctat
     9121 tgcattaata attttaatat aatataatgt gccagaggtt ttgaatggag tttgaagatt
     9181 tcttaccgtt aatcttgggg ctgtttataa tttgtggagt actactggca ttttactgga
     9241 tactgaagaa aatgacaggc aaagcaaata ggttgtcagc gggtaaatat gcaagaataa
     9301 tagatagaat agctatatct caggataaac agattcaatt agtggaaata ggagaatatg
     9361 ttttggtaat agggatatca tcttcttcag taaaccttct ttacaaagtg aaaaaagaaa
     9421 atcttaaaca attcgagcag gatatatcaa atcctcagtt taaagacatt ttttcgaatt
     9481 tcttatcaag atcaaaagat aacgaaagga aactttaatg gcaaaaaaaa gaaaaatagg
     9541 tatatttgcc actttacttg tcgcttttac ccctttttcc gtaatagcag cagaagatga
     9601 ccttacagaa gtactagagc agggactgga tgtcttatcc ggatattcga atttactgcc
     9661 tacagtaatt attctgctat tattaactgt attaccgacc attcttcttt taatgacatc
     9721 atatacgaga attcttgtcg tattatcatt tactagaaat gcaatgggta ctcaacagat
     9781 gccgccaaat caggtattaa taggtatttc tctgattctg acgatattta taatgtcacc
     9841 aaccataggt cagattaagg acgtagcata tgacccatat gtaaatgagg aaatgactat
     9901 tgttcaggct cttgaagcag cagaaatgcc attaaaggaa tttatgttta atgaagcaca
     9961 gaccgaaccg gaaagtataa atatgttctt gtcgctgtca ggtgttgagg aaactcctga
    10021 aacacttgaa gaaatgccta tgagtgtttt aatacctgca tttataacag gggagttgac
    10081 aaaggccttt aagataggat ttatgatata tctgccattt attatgatag atatgatagt
    10141 atccagtatt ttgatgtcta tgggcatgat gatgcttcct ccatctatga tatctttacc
    10201 ttttaaggtt ttactttttg tacttgttga tgggtgggaa ctgataatga agactcttat
    10261 catgagcttc ggttaaactg aaaaggagaa ataatggatc aggctttggt ggttaactta
    10321 ataaaagatg ctttgacagt tgttcttaaa gtcgttttac cagttatgat tattgctatg
    10381 gtaactggaa ttataattgc tgtttttcag gcggcaactc agataaatga gcagaatatg
    10441 gtacttgtac caaaaatagt tgtagttttt ttaggattaa tactcttagg gtcatggatg
    10501 atcggtcaga taacggaata tgcgagggag ctgtttaatc agatattatc attgatttag
    10561 gagaggtatg aacagtcttt cagtcgttta tacaaatata gatgtattct tacttgtgtt
    10621 agtaagaata gcgtcattta ttttcgtttc acctgttttt ggaagaagag gaatacctgt
    10681 aactacaaaa ataggattaa cggctgtttt aagtgttgtt attatgcttg gatttgagac
    10741 ctttccagat gtagctgaaa tgggaagcgg tatgtatctg gggctagtga taaaagaagc
    10801 cctcataggt atttcaataa gtttcataac tcttattttc tttacagtct tttatggtat
    10861 cggccagctt gcagatatgc agatagggtt taatgtaggt ggtatttatg atgctcagat
    10921 gcagatgcag gttccactta caggtaactt attttaccta atgggatttc taacctttct
    10981 ggttttaaac ggagcttcaa aactggtttt tctaatccat tctatgtatg acagtattcc
    11041 aatagtcggt agcagtctta atagtacttt ttatgaaatt ttcttcaatg cgtttacttt
    11101 tacatatgca tattccgtcc agattatact acctttaata ctgttaattc ttgtaacaca
    11161 gtttatatta ggagtaataa ttaaattttt accccagatg aatgtattta taattggtat
    11221 acctgtgaaa atagcgatat cacttattat tttgaatttt atgataggac ctctcttcca
    11281 aatgatggaa gtgtattttg accgcatgtt tgagagtgct atgatgctga taaggagttt
    11341 gagctgatgg ctggtgaaaa gacagaaaaa gccacactga aaaaaagaag agatgccaaa
    11401 aaggaaggcg atgtacataa gagtatagaa gtaagtaata ctttttcttt acttgttatt
    11461 acattgctgg taggtgtata tctcagtcat gccggaacat cactagttct gtttcttcaa
    11521 agttcactat atattacaga aattgaagta gtttttgatt tcaggaaata ccttaaactg
    11581 ttttttgata cagcaggtgt tttcatgata gcattactgg caatgggcct tcttacaaac
    11641 tacatgcagg tagggtttct gtttacatca aaaaaattaa aacctgattt aaagaaaatg
    11701 aatccaatag aaggattaaa aagaatattt tctaaaaaaa cattatttga actttttaaa
    11761 aatctcggaa aaatggcact tataatcata ttgtcatact cagcacttaa taaggctatg
    11821 ctggaatttt cctatcttag cttttatgaa atactgcctg cattacacaa agcattatca
    11881 gtgatgataa gcatactgac cagtatatta ataggacttg ctactttggc agttatagat
    11941 tttatttatc agtggtttga ctatgagaaa aaaatgaaaa tgacaaagca ggaagttaag
    12001 gatgaattta aaaacactga aggagatccg cagattaaat cattcattaa gagcagacag
    12061 cgcatgatag caagaagacg tatgatgcat gcagtaccta ccgctgatgc gataataacc
    12121 aatcctacac atattgctat agcaattaaa tacaaacaca aaaaagacac agctccaata
    12181 gttgtagcaa agggacaagg gctcattgct gagaaaataa agcaaattgc taaagaaaat
    12241 aatatatata tagtagaaaa taagccactt gcaagaattt tatttaaaaa agcagagatt
    12301 ggtgacttca taccttatga acactacaaa gcagttgctg aaatacttgc atatgtttac
    12361 aatataaaga ataagaaaga acagggtgat caaaattaaa agcaaattta aacaaagcat
    12421 attagcatta atagttatag gagtaataat gataatagtt gtaccactaa gtacgactct
    12481 gctggatatt cttcttatta tcagtattac attttcacta ataattcttt taacaacttt
    12541 gtatgttaaa gaacccttac aattttcaat ttttccaatg cttcttctta taactacatt
    12601 attcagactt gctcttaata tatcttcaac caggataata ttaagcgaag ggggtatagc
    12661 gggtaaggtt attactgctt ttggtaattt cgttgttcag ggaaatattg ttgtaggatt
    12721 agttatctat cttattatta tgattatgca gttcatagta atcacaaaag gcgcagaaag
    12781 agtttccgaa gttgcagcca gattcacgct tgattccatg ccaggtaaac agatggccat
    12841 tgatgctgat ttgaatgctg gtcttataaa tgaaaaagaa gcgaaagaaa gaagaaaaaa
    12901 aatagagtat caggcagatt tctatggatc tatggatggt gcgacaaagt ttgtaaaagg
    12961 agatgcaata gcaggaatta taataacatt gataaacctg gtaggtggaa tagtaatagg
    13021 tcttacttct gaaggtatgg atttctcaca ggttttggaa gtatactctt tggcaactgt
    13081 cggagacggc cttgtatcac aaatcccatc actaatgata tctaccgcta ctggtatcgt
    13141 tgctacccga tcatcatctg accaggattt gaatgagcaa ttctcacacc agttgttttc
    13201 tgattacaaa gttctattta taaccggagg agctttgctt cttcttagta ttgttccagg
    13261 ctttcctgga ttctttcttg taactatagg aggactgctt cttgcattag gatatgcaat
    13321 ttataggaaa acaaaagttg ttcctgaaaa tgaacatgaa aaagaggaaa ttcaagcaag
    13381 aacagaaaga gaacctgtta aaacaaatat tatggaactg ttaaatgtag atccgataaa
    13441 aatagaatta ggttattcgt taataccatt tattgatgaa tctcaaggag gtgatttact
    13501 tgagagaatg ataactatca gaaaccaatt tgcaggtaac atgggaatag tgatacctgt
    13561 cgtaagattc cgtgataatt tgtctttaaa atcgaatgaa tactcaataa tgattcgtaa
    13621 taatgtaatt gaaaaagggg ttgtgcttgc tgactatatg cttgctatga gttcttctag
    13681 tgttgaggaa gccgatttag aaggaataga cacaaaggat cctgcatatg gcatgagtgc
    13741 aaaatggata accaaggata tgcaggataa ggcacagatg ctggggtaca ccgttataag
    13801 cccctcctct gtaattgcaa ctcatatatc taaagttatt aagaaacatg catatgaaat
    13861 aatcggaagg caggacatcc agaaacttgt agataattta aaggaaagac agagtgtcct
    13921 tgtagaagaa gttatcggaa aaaccgtatc catcggacag ctacacaaag ttgtcatagg
    13981 tcttttaaaa gaagggatac cggttaaatc acttgatgtg atcgttgaga cattaggtga
    14041 ttatgccaaa acaacaaaag atatcaatgt actgatagaa tatgtaaggc aggctttaaa
    14101 aagagaaata actcagatat atgcaccaaa tggaagaata gatgtcataa cggtaaatca
    14161 tgatattgag gatatggtaa caaaatcaat tagatctaca gaatcaggcg gttatttggc
    14221 gcttgagcca gacaagatta agcatattat aaaagctgct aatgaatcga ttaacaaagc
    14281 agttacagca aatatagatg cagttattct tacttctccg atagttagga tatattttac
    14341 tgagattatt catcaaatag atgaaaatat tgacgttctg tcatataatg aaatagagaa
    14401 caaggtaaag attcaaatta ttggaagcat atcgttacaa taggggtggc tcatggataa
    14461 gacaaaaata tggaaggaat acatcaatac tcaaacggtt tcaagcagaa acctgattgt
    14521 tgagcagtat gcatatcttg ttaaaaaagt tatatattcc attccttgta ccaggattgg
    14581 aatgatagaa gaggatgatc tttatagcga aggcgtaata ggacttatag atgctgttga
    14641 gaaatttgat ccggataaga atgtcaaatt tgaatcatat gcatgcctta ggataaaagg
    14701 tcagattctt gactatatga ggaaagtaga tatcttatca agagatgcca gaaagaaaac
    14761 gaaagaagta gcagactata taaatactta cataaaccgt tacggcaagg aaccctctct
    14821 ttcggaaatt tccagttcgc ttaacataag tgttgataag gtcaaaagga ttcaggagga
    14881 agatgcttta tcaaatatgg tttctttcga atcttttatc gaagataatg gggacataat
    14941 ccaaagcaaa aaagaggaag aatatccaga agaagctctt gacaaatcac agcttctaga
    15001 agcaatggct tatcatatag ataaattaag tgagaaagaa aagacaatac ttaatttata
    15061 ttattacgaa gaattaacat acaaagagat agcagagata atggatttat gcgaatccag
    15121 gatttctcag ataatatcat cagttttagc aaatttaagg gaaaaattaa taaataacat
    15181 ataaagaaac ctaataaaca gccgattaaa taaatgagag ccactaaagg aggaaataga
    15241 tggatatcgg ccttcaacaa cttggaaaat cattactggc aacaaacagc catcttgctg
    15301 ttatatcaaa taatatagca aacagcgcta ctgacagatt taaaagcgat actctttcaa
    15361 taagatcatt cagtgactat tttattcatg aagatcacat ttcaaataaa gagaatcttt
    15421 acgagataac tactcatact gattttacat caggtgagat ttataaaaga aacagtaatg
    15481 actttgcttt ggacggaaat ggttttatag cagtaatata caatgataac ttatgttatt
    15541 caagaggcgg aagcttcaat gtagatgcag atggttatct tgttgaccag catggctcat
    15601 atatacttgg aacacagggt aatatacttg ttaacaatgc aaatgttgaa atatcaaaag
    15661 atggtacagt tacactggac ggaatatatg cagatacaat agagatatct gattttgaag
    15721 attacagatt agttaacaaa acagggaaag attattatgt ggctgagcag ggagcaaatg
    15781 tgcttcaaag taatacaaat cttttgcaag gttatagcga aaaatccaat gtcagtataa
    15841 ctagtgaata tttaagaatg atagagatga gcaagaagtt tgaaacagga caggccgtta
    15901 tacagattct tgacgagatt aacggtaaag ccgttaataa catagcggta ttctaggggg
    15961 cataatgaat aatgcatatt acatagcagc caattcaatg atgagccagg aaaaagtatt
    16021 cagtacaata tccggcaaca ttgcaaacat taatacaaat ggctataaaa agacttctgt
    16081 cagtttcagc gatcttatgt actcatttca acaaacagca tcatcgtcag aatacagtat
    16141 tggaagtgga ataactgtta acggatataa taaggatttc caatatggca atttgaaaga
    16201 aacaggaagt aatttagata tagcttttga aaaagagggt tttctgacat tagccgacgg
    16261 ggacgaaagg tactatacta gaggaggtaa ttttactctt tctgctactg gtgaaggtta
    16321 cttcatatca aataatgatg ggtttttcct tctagattca gagggcaata gagtacaaat
    16381 atcccaagag gaaagagatt tcgaaataga cagtaacggg ttaatgtctt ttaagaatag
    16441 tgacagaata gtaaatttgg ccattacgag ttttgaaaat cctgaaaaac tacagccttt
    16501 tggaaataat ttatatatag ctggattaga actaaaggaa aacactcaaa cggccgatat
    16561 aatattaaga caaggctttg tagagcaatc taatgttgat attgcgacag agatgactga
    16621 tcttatacaa gcacagaggt tataccagat gaattccaag attctgcaag taatggatga
    16681 aataagcggt atggccaaca gattacgtaa ataaatgagg agtatgaaat gaaaattaat
    16741 cctttaagct catatgatgc aattaacaaa gttaataaga atgcagaaaa gagccagata
    16801 accaaaaccg atatgaatgt caatgatgat gtcagaattt cagaccaggc taaggcaatt
    16861 gataagtata ttcaaaaggc taaaacctct gatattgacc gttctgaatt agtaaacagt
    16921 ttaaaaaaca aagtcagaag cggtaattat aaagttgata gcaaggtgtt atctgaaaag
    16981 attattgaat cagtagttaa gaagtagttt aagcatttaa tacttaaacg aaaacagagc
    17041 tttaattatg aagcatattt ttttgtaccc taaaggagag tgaaatgacg aatcttttcg
    17101 aagaaataat ggatttatac tcaaaacagg ttgatataat cagtcagttt aatgaggttt
    17161 cacagcaaaa ggtaaaaata ctggaaagta atgatacaag cttgttaaac agcataatca
    17221 aatatgaaga agctcttctt atgaaatttt cagcagttga aaacaaaaga cagagaactt
    17281 tggatatagt aaaagatcag tatggggtta aaaagattac taaaagaatt atgagaaaac
    17341 atcttaatag taatcagatt aagcaagcag atcaactgag taaagaattg aatacacatt
    17401 tagaggaaca gcataaactc aagcttttaa acatgaaaat tgttgaaacc agattgcatg
    17461 aattaaaatc tgcactagat ataattgaaa aaaggagtaa tgaatattta gaaccaaaag
    17521 tcttactgga taaaaaggta taattatgag tacatttagt ggaattgaaa tagtcagaaa
    17581 aagtctatat gcaaatcaaa aagctattga tgtaacaggt cataatattg ctaatgcaaa
    17641 tacaaaagga tattcaaggc aggttgtaag gtttgagcct gtttcggaca attccaaaag
    17701 tctttttacc ggtacaagaa cttcaaatat aggaagagga gttgttcttg cagaagtcaa
    17761 gcaggtcaga tttgattatt atgacagtat gtatcgtaag gaaacaggaa ttcagtcaga
    17821 gctttcatct aaaagctctg catttatata tatcaacagt ctagttagaa atgggcgtga
    17881 cgattcaata ccaaatatgc ttactgattt atttaattct atggaaaatc ttgcatcaaa
    17941 tgcacaaaat atgactctta gggaagaagt aaagcaaaat gcaattcttt ttacggaaaa
    18001 tcttaatatt acagcagatt cacttatcca atacaaaaat gaattaaatg aggatatcaa
    18061 aacatatgct tacgagataa atgataaatc aagtaagatt gtagaactga ataaaatgat
    18121 atttgaattt gaagcaacag gaaaaacagc taatgaatta agagatgaaa gaaatctaat
    18181 agtagacgaa ttatcagaac ttattaacat cacagttaat gaaaaagata atggcgaata
    18241 tcaggttatg actggagggt attgttttgt agatcactat acagtccatg aaattgaagt
    18301 cagggataat ttagtaagtc caacaacaga cggtttctat aaccagctat actggaagga
    18361 tacaggaact aaactttcac tgacaagtgg aactgttaaa gggatgcttg atataaggga
    18421 tgggaatagt caggagaata tgggtattga ctatatactg acacaattgg ataattttgc
    18481 aggttctgtg gttgaacaga ttaataatat aaacagagca ggttacacta tgccttatgc
    18541 ttcaaatgtt tcaagcacag gagtggattt ttttgaaagt tctaatgtta aagcttcaaa
    18601 tataagatta tcagatgcac ttattgaaag tgcagctaat atagcagcat ctgatcagga
    18661 aatcactgat gatactgata ttgcaaataa cagaaacctt atgaattttg tagcattaag
    18721 agatagtacc gacattgtat atgaaggtaa tgatataggt aatcttgaag attatataga
    18781 gtcagttttc tcaaaagtaa caatagcaac aggttatgca aaatcaagag cctcaagtca
    18841 ggaagaaata atttcatata tttcaaatca gaaagattca gtttcaggcg ttagtataac
    18901 agaggagact attaatctta ctgcatatca aaaagcatat gaggcggcag caaatctcat
    18961 gaaggtaatt gatgagatga tggatactct catatcaatg gtataggaga tgaattatga
    19021 gaattactaa tggaatgata gcaagaagat caatgagaga tattgaaatg gcatataaga
    19081 aattatctga tgcttcatac tctttttcca ctgggaaaaa aaatgagaac acatatgacg
    19141 atcctgtagc ggctaacagc cttcacaggc tgaagaatga caaaaaagaa atcacaaaat
    19201 actacgcaaa tattgataat gcaagaacag cagtttcaga tatggaaaca gctctttcgg
    19261 atattaatcg ctgtatgcag aaaatcatgg aactaagtac taatgcaaat aatgcgacat
    19321 tatcagcaac ggcaaaagaa tctataagcc aggaaataca gcagtataaa gagcagatta
    19381 ttactgattt caattataac agtgcaggaa tatatatatt tggcggtttt aataccaaca
    19441 gtaaaccaat aagcgtaaat gaaggtaatt atttatataa tggaaccgat attctgacaa
    19501 tgacagaaga acagcattta caagcccttg gtgaaaaagt aatggtcaat gttagcaaag
    19561 gaatgaccat ggatatcaga atatctgctc tggagataat cggttctgga gaagaaaata
    19621 tacttaataa cattgatgct ttatccgatg taattaaagc aggagaattt gacagcacat
    19681 catttgaatc gttaaacagg attcttgagc aaaattacga taaagtatta actaatataa
    19741 caaaaacagg cgctgcagca aacagacttg attcggtcaa aaaacaactg gacagcaggg
    19801 aatcaacact taatgacagg atatcggatt tagaggatgt agacacagag aaagctattt
    19861 taaattacaa attagcagaa caggcatatg aagcgtcatt atcagcttca gtatcagcaa
    19921 tacaattgtc attacttgac tttataagat aattttaact taaggtggag aaaaatggaa
    19981 aaggcatacc cagagaaaat agtttttaaa aaagatatat atggcttcaa ggatattaaa
    20041 aaatattcat tatcaaaagc ttcagaaaat gataataatc cttttaagat actgaaagca
    20101 gaagatgaga gtgtttcctt tattttactt gaccctacgg ttatagataa atcatataat
    20161 ccaattataa atgataaaat tgcaagactt ctaggaacaa ggaccgctga ggatttagca
    20221 gctttatgta ttgttgttat accaaatgat attaaaagga tgacaattaa cttaagaagc
    20281 cctataatta taaatgtaag caatggatat gcgatacaaa caatactaga agacgaaaga
    20341 tatagtataa ggcataaaat actggattgt taaagggaaa aagaaatgtt ggttatatca
    20401 agaaaagctg gagaatctat aattattgga gacgatatag aggtcgttat cattgaagcg
    20461 caaaacggca aggtaaaaat tggtgttaaa gctgataaca gtatccggat tttaagaaaa
    20521 gaaataattg acgaagttac aaaaaccaat atacaggcat cagagaaatt aggtagtatt
    20581 tcaattgaca cgataggatc ccttataagc aaggaagcaa aagaatgagg tatgtcccca
    20641 caacatttct aaaagcggat atggtagtag gcaaggatgt ttacgggaga tacggggaag
    20701 tataccttaa aagaaacacc aagcttacat tgaagtatat atcgcaactt gaaatactgg
    20761 ggtatctggg tatatttatt gaagatgaga tatcagaagg tatagaaatt aaggaaatcg
    20821 taaatccaag agtcagagtt gaagcaatca acaaagttaa agatgtatat acagcaatta
    20881 aaaataataa taaaggcata ctcaacaact ggaaagaaat agatcagtta acagataaca
    20941 taatatccaa tatattaagt aatgaagata cagtatataa tatcatgtat atgaaaacgc
    21001 atgatgatta tacatacacc cattgtgtga acgttgcgtt aatagtagca atagtagcaa
    21061 agaaaacaat ggttagcaaa gcgattctta tggatttatt taaaagcgct ttgatgcatg
    21121 atatcggcaa actctttata ccgctttcga tattgaacaa gcccggacgt ttaagtacag
    21181 atgaatatga gcttataaag cagcatcctc tgaaaggata tgattatctt gttgaacagg
    21241 gatgtctgac cactcaggga agaagaggtg ttttaatgca tcatgaatat cataacggta
    21301 aagggtatcc gtttgggata tcagatgccg gaataaataa gtatggaaag attttatgta
    21361 tatgtgatgt ttatgatgct ttagtttctg acagaccgta tagaaaagca tggagccagt
    21421 cagaggcatt tgaatatata atggctaaca cagggagcat gtttgatccg gagataatga
    21481 aaatatttat agataatatt acgccatata atcctggtac aattatacag ctaagtaacg
    21541 gtgcaaaagc aatagtagta aaaaacaata aaggacttcc tcttaggccg ataatcagaa
    21601 ttttcgaaga agacagtatc aaagtacagc ttagagatgt agatttaaaa gaaaatagcg
    21661 attatctcaa tattgtaatt gataaggaaa taaattatta atatataaat atgataagca
    21721 acatatattt taaattctaa caaataagta aagaacctaa cagtcaatat tagcaaacaa
    21781 aaaaaacatc aaaaaaataa aaactaaaaa atttactaaa gttatataag atttatccga
    21841 ttaaatagtt gtgaagatac cggccggtat tgtagcaaaa aaatatggga aaggattccc
    21901 gataaaaatt caaggaggat atcatgagaa ttaatcacaa tatagctgcg ttaaacacat
    21961 ataaccagtt atcaaagaac aacgagaccg cgaacagcgc attaaagaaa ctatcgtcag
    22021 gtcttagaat caaccaagct ggtgatgatc ctgctggtat ggcgatttca gaaaaaatga
    22081 gagcgcagat caacggatta aatcaggcaa acagaaacgc tcaggacggc atatctctta
    22141 tacagacagc tgaaggcgct ttgaatgaga caacatcaat acttcaaaga atgcgtgaac
    22201 tggcagttca agctggtaat gataccgcaa cagcaaacga cagagcaact atacaggctg
    22261 aaatggacca gttagctcag gaaatcacaa gaatagcaga cacaactaaa tttaacggtg
    22321 aagcacttat gtcatcaagt catgtatttt caggcaactt acagattggt gctgatgcag
    22381 gacagactct tgatgttcaa ttgacacaga tggatgcttt gacaattggt gttggtatag
    22441 gaagcgatgc tcctactctt gcagcagcag agtatacaga tgtagctgga actttctcag
    22501 ctgacggcgg aacaattgac acagcaggtt atacaacaaa ccttcagtct cttgaaatta
    22561 cgtttgatgt agttaccgtt ggaaatggaa cagacacagc tggtacagct aatgttacag
    22621 taaatggaac aagcgtattg ctgacagcag attctgtagc agcagatggt acttatacac
    22681 tttcacacag tgacttttct gatattattg catctggcga atcaatcaca atagatgcag
    22741 gcgctgctct tgaagctgat atggttacaa ctataactgt tgatgcactt cagaatggtg
    22801 atcttgcagg acttgatgtt tcatctggtg cagcagcagc agcagcttta acaagcattg
    22861 actctgcaat tgatatggtt tctactcaga gatctaatct tggttcatac accaacagac
    22921 ttgagtatac aataaccaac ttagaaacag cttctgagaa catgacagca gctgaatccc
    22981 gtatcagaga cgtagatatg gcatcagaaa tgatggaata cacaaagatg aatgtcttga
    23041 gccaagcagc tcaatccatg cttgcacaag caaatgcaca gcctcagaac gtattacagt
    23101 tactgaacag ctaatcggat taattagaaa ttcccgccgg ttattaatgc cggcgggtat
    23161 tttcttttaa tcataggcta gttcagagat tagaggtata ttatgaatga ctcatatata
    23221 gattctttaa caggggtttc agctgtgagg caggttgaga gccaggtaaa caaagaaaac
    23281 cagacagtca gacaacaaac agaaagcaga tctgatttta agacatttaa tgaagaactt
    23341 cagttttgtg acaatgaaaa gcaactggat gaacagttag aaacgcttaa caatactctg
    23401 cgaaatgaaa ataaaaaagt agaatacagt atttatgaag aaacaaatac agtaatattc
    23461 cgtgtaatag atactaaaag cgaaaaagtt attaaagaaa taccatcaac aaaattaatt
    23521 gatttttcag tagcagtatt ggagagattc ggattaattg ccgataagga agtataggag
    23581 agcatttaca tgacagacca agtatcaagc ttaaaatatt catcaagtaa gatatcaggc
    23641 ttagcatcag gactggatac tgaggctatg attgaaagct taatgaaagt tgaaagacaa
    23701 aaggtcataa agattgagca ggagcttgaa tatactactt ggcagcagga tgcatacaga
    23761 gatgttatac ataatctttc agattttact aaatcacagt ttaacatatc tgataactca
    23821 aaaaatattc tttccacaac aaatctgaaa tcagtaaata ttgaatcttc agaatatgca
    23881 tcaatactgg caaatggtga tgcaaatata ggtgattata cgataaatga gattgtcagt
    23941 gtagctaagg ctgcaacaat cagcagcagt tcaacagtta aaaacggaat acagggaaac
    24001 aaggatttgt ctttaatgtc aagtatagac gttgagggca tgtcctttta tgcaagactt
    24061 gatggagagc agtatgagat aagtttcagc cagaattata ctgatacagc ttctttagtt
    24121 tcagacattc aaagccagtt tgataatatt ttcggagcag gaagagtaac agcaggtatc
    24181 aatgtggacg gagcattgca gtttaatgct tccggcagta tactgcagat aatagataac
    24241 attgagtcag atacgaagtt ggaatatata ggcttgagat ccggagatat gagcatattg
    24301 aatttaagag gatcattaag cgaaaatttc ggatatgaag aaaatgtatc attcagcata
    24361 aacggtactg aatttaattt tacctatgaa gacagtattg aaaatattat gaagactgta
    24421 aatgagtcga cagcaaacgt caaaatgact tatagcagta taacggataa gataactata
    24481 tcatccacaa agacaggcag taacgctgaa atagatatac agaatctttc aggtaatttc
    24541 tttggaccaa gtggaatagt taagatagac cagggaataa caaaaaacgg ccaggatgcg
    24601 atgttctatc tcaatgacga aagtaaagag aatctgataa tcagaagctc aaatactttt
    24661 acaatagaca acgtcactta tacattgaaa gaggaaacag taacacccat tgatttcaaa
    24721 gttgaaaaca atgaggatgg tgtttttgaa tcaataaaat catttattga ggaatacaat
    24781 agcatagtag aagaactgac ttctcatgta acgcagaaaa gagattatga ttatgaaccg
    24841 ttaagtgatg agcagaaaaa ggaaatgagt gaagaccaga ttgctgactg ggaagaaaaa
    24901 gcaaagcaag gacttctaaa agcagacaat actatataca gtatgcttaa tgaattgaga
    24961 acagcattta ttaccggtgt agagaatata agcatgacat ttggaagtat aggaattaat
    25021 acatcaagtt ataccaataa tggaataatc actattgatg aaactgttct taaaggtgct
    25081 atatcaggaa agtttgatga tgttgtaagt ttgtttacaa gagaatcaag tatcagttat
    25141 ttaagagatc taagcagtga agacgcagct gaaagattcg atgaaagcgg tttctgccag
    25201 agggttaatg atattgttaa aaaatacata acaacagcta gagataagaa cggcaataag
    25261 ggattacttg tagaaaaagc aggaattgaa aatgatgcat ctgaaggaac aaacgcactt
    25321 acaagaaaga tagatgaaat aaaagagagg cttgatagag ctaatgattt aatggataag
    25381 aaggaaagag gttactggtc acaattcagt aatcttgaag tagcaataaa caaattaaat
    25441 acgcagagcg gttacgtatc atcaatgatg gagcaataat atgaaacaag aaaatatttt
    25501 aaaagaatac tcagacaata acatattaca atcaacacca gaaggtctta tacttcttct
    25561 atataacaag tgcataagca atatagaagc agctaagtac tacaatggca agaagaatat
    25621 tgagaaaacc aataaatatc ttcaaaaatc tcaggatata ctatattatc ttcagtcaac
    25681 tttggataac aagtttgaaa tatcaaaaca acttagcaca atgtatgact ttataatcaa
    25741 tcagctaaca gttgctaata taaagaaaga ttctgaaaaa atgagtatag ctttagattt
    25801 tactattcaa atgaggaatc tgtgggttga agtaacggaa gagaacagaa aatacaagag
    25861 caaagcagtt taacatggca tatgccaata tttaatgaat aagtgaagtc tgcaggtaaa
    25921 tcttaataat aaaacataaa agaaacgcta aattaaaaga gcgttctttt atttgatcta
    25981 atttctttat agtgcttttt caataacgag aaagtagaat ccgatatatc atgctcaatt
    26041 ttacatgcgt ctttgtatgc tgtgtcctta tcaacaccca aggccataaa gaaagcagca
    26101 agaagctcat gcttttcata tattttctga gcaattgata aacccttatc tgtcaaggta
    26161 attccagaat cataatccat gtttattaaa ccttcatctc tgaattgttt catcattaca
    26221 cttactgtag gtttagaata gcccatatat tttgcaacat ctatagaaat aactcttttc
    26281 ttgttcatca gaagaaacaa tatagtctca aggtacattt cctgtgcttc acttacttcc
    26341 ataaatctct ccaatctgaa aaatggttag gtagtattaa ctataattag tatagactaa
    26401 ccattttagg ttgtcaacac acataatttt tcccgaaaat ctagattttt actacaaaaa
    26461 caatttttac tatatataga tatataaaaa cctatttaaa agaatatatt ttatacaaaa
    26521 ttagtattcc gtattaaaga acggaaagaa aagaaccgat atataaaaca taggatgtat
    26581 cactggtatt caaccagttg tataaataag caggggggat tatgacaaat aacacattta
    26641 atgatcatat gctggaagtc ttcctttttg aaactaatga gcttatggag cagttagaag
    26701 agcagttgct tcatattgag gattcaaaaa agatgactaa agaaaatatt gatgagattt
    26761 ttcgaattat gcacacagta aaaggctctt cagctatgat gcagtttgat gaaatcagtc
    26821 atcttacaca tagtcttgaa gacttgtttt tctatatacg ggaaaacggg cttgaaggaa
    26881 tcgatgctga tgaaataact gaagtaggat ttgtcactat tgattttatt agaaatgaag
    26941 ttatcaagct tgaacaaggt aaggtgtcgg atggagatgc aggttcattg aaagaacgaa
    27001 ttgttttaat ggtcgagaaa tttaaaaagc caaaaaacaa tggtaataag aatgaggatt
    27061 tgaaagaatt attaaaggaa tctatggagt cagatgaatt agaaaatatt agtgacgatg
    27121 tgattgagaa tagcgatact gcttatgaag aagataagaa taaaagtaat aaaaaactct
    27181 atgaagcaaa ggtaattttc gatgaggact gtggaatgct gaatataaga gcatttacgg
    27241 ttataagaaa tattacagat tactgtgata atttaagtta tcttccagaa gaacttatgg
    27301 acagtgacga tgcttctgat attattgcta aaaatgggtt tatcattcgg ttctcatcgg
    27361 atcatgaata tgatgaatta aagaatatat taaaagaatc attatttgtt tcagacataa
    27421 agcttgacat ggtttctgat aatgttgaag aagaaaaacc aaatgatgat gctaaaaatg
    27481 gggatattga aaaaacagat gataatttag caattgatgt gtcaggaagc tcacataaaa
    27541 agagtattat aagcgtcaat attgaaaaga tggataaact tatggattta gtaggggaga
    27601 tagtcattac tgaatccatg gtaataaata atcctgatct caaaggcata caactggata
    27661 acttccataa agcggcagca cagctgaaga aacttaacaa tgagcttcag gatgttgtta
    27721 tgtccataag gatgcttcct attgcggcaa ctttccataa aatgcacaga atagtcagag
    27781 acatgaataa aaaactaagt aaggatgttg aattattcat atatggagaa gaaacagaag
    27841 tagataaaag cataatagat aatcttggag aacctcttct tcacttagtg agaaattcaa
    27901 tggatcatgg aattgagagc actattgata gaaagaaagc tggaaaatct gaaaaaggca
    27961 aaataatact tgatgctcag aataatggaa gcgaaatcac tataagagtt agtgatgatg
    28021 gcaaaggcct tgataaacat aaaatttatg aaaaagcaaa agctcatggg ctggtagcaa
    28081 aagaagaaga agaattgacg gataaagaaa tttattccat gattttcctc gctggattct
    28141 caacaaataa agaagttaca gagttctccg gaagaggagt aggacttgac gtggtaagaa
    28201 aaacaataga aaaacttggt ggaacaataa cagttgacag tgccattaat gaaggtactg
    28261 cttttaatat gagaatacct cttacattag caataattga tggaatgaag gtatctgttg
    28321 gaaagtcaat ctttacaatc cctataacct cgataaaaga atcaatgaaa ataaaaaatg
    28381 aggatattgt aaaagatact tctgacagag aaatggtaat gataagagga aaagcatacc
    28441 cggttattag attgcatgat cattatataa ttgatactga agtccgggat ttaacagaag
    28501 gtattctggt tatggtggaa tcggaggata atgcgtattg tatatttgca gataccataa
    28561 ttggtgaaca gcaggttgta gttaagccaa tacctgcata tgtaggtaaa tgccagaacg
    28621 caaattcggg aatagcagga tgtgcaatac tggatgattc aaatattagc ataattattg
    28681 atgttatggg tcttcatggc caaataatca aatagaagga gagaggaaat ggataatgat
    28741 aaaaatatga tccagataga ggaagataca caaaaagaca gatatctaac attcagtatc
    28801 ggaaccgaat cttttggtgt ggatatcaag tatgtaaccg aaataatagg aatacagcct
    28861 attactattg ttccggaggt tccagattac gtaaaaggga ttatcaactt aagaggtaag
    28921 attattccgg tgatagacat caggctgaag tttaagaagg aatctattga gtacgatgac
    28981 agaacctgca taattgttgt agatgtggat gatgtaacag caggatttat cgttgataat
    29041 gttacagaag taataactat agcaggagat gatatagtcc cgcctccgga gtacaaggag
    29101 tgcttccaga ataactacat taaaaatatt ggtaagacgc caaatggaat aaaacttcta
    29161 ctagactgtc agatgatctt aagcggggag attatctaaa gctaatgaag tgtaaatgta
    29221 attaaaagta acaaaaataa atgaaaaata catataaaac aaacaggagg attaaaatga
    29281 agtggtattt aaacatgaag atggggaaga aactggttat agcattcgta cttgttgcaa
    29341 taatcgcagg aatagtagga tttcttggat attcaagtgt tcaggacatt agaaccagtc
    29401 agacatacat agctactgta actatgccta ggattgaaaa tcttataaca gttagagaaa
    29461 aagttacagc tataatgctt ggacaaagag gactgatgat ggaaaagatg aaggctcagt
    29521 ctttaagaga tgcgcaatat gactggattt ctgatgccag agtggatctt ggtactgcat
    29581 gggataacat tatgagtttg gaaatactgg attctgaaaa ggaacagtat gccaaaggag
    29641 aagaatatgt tacaaatttt ttagcagaag cagataaact tgatgtgatg gctaagaaaa
    29701 aagatgaact gattcagggt ggcgctgatt atgatgatat ggctgttatg aatcttgatg
    29761 agcagacata ccagcagtca ttggttgtaa gagctattta tcttgaattt gataaactga
    29821 ttgaaacaat aatagttagt gataaagcaa ttgctgatga aacatatgcc caagcagaac
    29881 aaggagctga tcaggcagcg gtattcctta tagtaatcat aggtgtggcc attctagtat
    29941 caataatcct tggagtattg ataagcagga tgattagcaa accaattgat gaaattgtag
    30001 gttatgctaa taatattgct caaggaaggt tggacgatca tattgaagtt aagacaaagg
    30061 atgagttggg agatcttcag agagcatttg gccagatact ggaatcttta aatactacta
    30121 tgaaagatat aaacgaggct gcagagcagg taacaacagg atcttctcag gtttcagatg
    30181 gtgcgcaaca gctttcacaa ggatctaccg aacaagcaag ttccatagaa gagattacag
    30241 catcagttac gcaagtcgct gaacagacaa aggacaatgc cagcaatgct aacgaagcta
    30301 acagggttac tcttgaagtc aaagataatg catcaaaagg cgacaaggaa atgataaaca
    30361 tgcttgatgc aatgaaagag attaatgatt catcacaaaa tatttccaag attatcaaag
    30421 taatagatga cattgctttc cagacaaact tacttgctct taatgccgca gtagaagccg
    30481 caagagcggg acaacacggt aaaggattcg cagtcgttgc tgaagaagtt agaaatcttg
    30541 cagcaagaag cgcaaatgca gcaaaagaaa caacaacact tatcgaaggt tcaatggata
    30601 aagtaaatgt aggaacaaaa atcgctcagg aaactgcaaa agctttagca gatatcgtaa
    30661 aaggtgttga aacagcaaca actatagttt cacagatatc aagcgcatca aacgagcagg
    30721 ctgcagcaat agaacagctt aatgaaggat tgaatcagat atccaaagtt gttcagaata
    30781 actcagcaac agcagaagaa agtgctgcag caagtgaaga gctttcatct caagcagaat
    30841 tacttaaaga aatggtaaac agatttaaac ttgcaactga tgatagcaag aaagagctca
    30901 gaaaagaaac aactgtaaaa aaggaagttt caaaatcagt tccaaaacaa attaagctgg
    30961 atgatgacgg ctttggaaaa tactaaaaaa ggggctatgc cccttcctaa aaaggggcta
    31021 tgccccttct ttaggcaatt ccagtactct tatattaaga gattgtaaaa agttattgca
    31081 cgattaggag gattaaaatg aagaaattaa agataacagg aaagctcgta atgatggtat
    31141 tgccttctat gctggtcttg ttcgtcgtaa tagtattttc agtattcaat aatttaaatg
    31201 tatataacag gacatacgaa aatatatatg atcagttata tgtaaacaca gaattgttac
    31261 ttaatgcaga cagagacttg taccaggctc tgcttgcaga acaggaactg tatattgagg
    31321 gtgatacact atctgaaact aagaaaaatg aattagtagc agattataag gaaaactaca
    31381 tacaagcgca agacaggata gtatcagtca gtaataattt aattaacatt tctgcattgt
    31441 atgaagattt cagccatgag gggtcaggga aaaacttaaa aacacttgtt gcagagttta
    31501 atactgactt tgacaaatgg gttgcaacat ataatcccga aacaaataaa ggaaattatc
    31561 ttacagcact tggttacttt gaccaggcaa gagaaaaaat tgattttgca agtcagttac
    31621 ttgatgacta ttcaaaagtt caagctgaag ttgaaagcag caacatgaca agaaacacag
    31681 tgctattcat tattttcggt gttgtaatat gtgcaatatt gacagttatt gctcagagtg
    31741 tttcaaaaac tattaaaaaa ccagtaatga cccttaaaaa aatggcagac aggctggcag
    31801 aaggtgatct tacagtaaac gagaatacaa attcaaaaga cgagttaggt gatcttcaaa
    31861 gagcgtttaa caaaactgcg ttaaatttaa gggaaacatt agcggagata aataactcat
    31921 ctgagcaggt agcagcagga tctacacagg tttcagatgg tgctcaggaa ctgtcacaag
    31981 gttctacaga acaggcaagc gctacggaac agatagcaga aactgtagga aaaatgcgtc
    32041 ttatgataaa tgacaatgct aaaaatgctc agaaagcaaa tgagatatct gtactgatgg
    32101 gagatgatgt cataacaagc aatgaagaaa tggataagat gctcgaatcc atgaaagata
    32161 ttaacaaatc atctcagaat atttcaaaga ttataaaagt tattgatgat attgcttttc
    32221 agacaaatct tctggctctt aatgcagctg tagaagcagc tcatgccggc cagtatggaa
    32281 aaggttttgc agttgtggct gaagaagtaa gaaagcttgc agcaagaagc gcaaatgctg
    32341 caaaagaaac aacaacattg attgaagaat caatccaaaa agtcaatgtt ggaactacaa
    32401 cggctaaagg cacagctgag gttctggaaa agatagttgt caacatttca aaaacagcag
    32461 atattgtaaa gaatatctca gaatcatcac aagtacaggc aacacaggtt gaacagttaa
    32521 atactgcagt caatcaaata tctgcagttg tacagagtaa ttctgcaaca gctgaagaaa
    32581 gcgctgctgc aagtgaagaa ttgaactctc aggtagtgat gttaaaagag atggttaaca
    32641 ggtttaaaat ttaaaaaagg agaaatacag tggctaaaat atatgaaaca gagtctgaag
    32701 acatgctaaa tgaaaataca agtaaagata tgtatttaac attcagactg aataatgaag
    32761 aatatggatt tgatatatct catgttactg aaatagttgg tattcagcct ataactttga
    32821 tacctgaatc agctaattac ataaaaggta ttatcaattt aagaggtaaa atcattcctg
    32881 taattgatct taggttaaaa ttcaacagga cagaaaaagc ttacgatgaa agaacatgta
    32941 ttatagttct tgcatatggt ggcactgatg tcgggtttat agtggataat gtttcagaag
    33001 tcgttacttt gaaggaagat caggtcatac ctctgccttc gtatgggtct gaacttagaa
    33061 ataattatgt caagaatatt ggcaaggcag caaatggaat taagctatta ctcgactgta
    33121 gcaggatatt ggggaaaaat gaattggaaa tgatagaggg tcttctgaca aataaaagtg
    33181 atgataaaaa ttagtgataa tgaatttaga agactggtga cttttgttaa gaaaaattac
    33241 ggcatcaatt tgtcacagaa acgtaattta attgaaggtc gcttaggtaa tataataact
    33301 ggcaaaggtt attctgaatt ttctgattat cttgattatg ttttcagtga aaagggaact
    33361 gatgaaatgg ttaatatggt taacaagctt acaaccaatc ataccttttt tatgagagag
    33421 tcttcacatt ttgatttttt tagacaaacg gtgcttccat atctgaagaa cagtaaaaac
    33481 aattacgatc tgaggttatg gagtgccgga tgctcttcag gagaagaagc ttatacacta
    33541 gcaatgatta ttgatgattt tttcagcggt tcaacggaac catgggaaaa aacaatgtta
    33601 gcaactgata tatccacaat tatgcttaac aaagcagagg acgggattta tgagcaatca
    33661 tcagttttga aaatgcctaa aagcttccag actaaatatt tccagcctta tggaaacggg
    33721 caattcaaag tagtccaaag cattaggcaa aagattgttt ttaaaaaatt caatctcatg
    33781 acagaaatat tcccatttaa aaagaagttt gatgtcatat tctgcaggaa tgtaatgata
    33841 tattttgata aagagacgaa agatgctttg ttgaaaaaat tcttcaacag tatcgaggat
    33901 ggaggttatc tgtttattgg gcaagctgaa tcaataacca gatcaagtac aggctttaca
    33961 tacgtaatgc cttcgatata caggaaggtg taatttatgg ttaatgatat aaaagtatta
    34021 atagtagatg actcaatgtt ctttcgggaa tatttatctg aaggccttaa aaagcagaat
    34081 gggattgaag tggttggatt tgcaaaggat ccttatgaag caagagatct gattcttgaa
    34141 ttaaagcccg atgttattac actggatgtt aatatgccta gaatgtcagg gattgatttt
    34201 cttaaaaaac ttatacctca gtatcctatt ccagttgtag taataagttc tgcaagcaca
    34261 aatatttttg atgcactgga tgccggagca gtagatttct tgaagaaacc ggatattaaa
    34321 gataaagatg gaactgtgtt ctttaaagaa ctggcaatca agataaaaat aagttctcag
    34381 gtaaaataca taaatgtacg taaggatgaa aaaagtaact tttcaaaagg ttctacagat
    34441 atacatgatg ttgataagta tattattgca atgggcgctt caacaggggg aactgaggcg
    34501 ttatttgatg tcataaaaag actaccggta aatttacctg gtattgtaat tgttcagcat
    34561 atgcctcctg tatttacaaa aatgtatgca gacagactta ataatcagac agatttcgag
    34621 gtgaaagaag ctgaaaatgg agacctggta aaaaagggaa ggattataat agctcaaggc
    34681 ggtatacaga tgcttgttgt aaaaagagcc ggagaatatg ttataaaact tagcgggaca
    34741 gagaagcgaa gcggacactg cccatctgtt gatgttttat tcagttctgt agcagaagca
    34801 gcaagagaga aatctgtggg tgttcttctc accggaatgg gtgttgatgg tgcaacaggt
    34861 cttttagaaa tgaagaaaaa aggagcatat acaataggtc aagatgaagc aacttctata
    34921 gtttatggaa tgcctaaagc agctttttcc ataggtgcag ttatggccca gcttcctctg
    34981 ttggatatac ctggaaagat aactaattat tttaataaga tttaggagat agatatgact
    35041 aatatagaca gtttcaatga atatcaggtc gatgttctta aggagatagg taatatcgga
    35101 tcgggtaatg cagcgacttc actttctatt atgttaagag gaagaatcag cattgatgtt
    35161 cctgacgtaa gaataattga tgttgatgga atatcaaatt tacttggagg accagagaat
    35221 caggtagcgg gcgtgcttgt ccttatgaaa ggtgatattg agggaatgtt attatttctt
    35281 ttagaaaaga gtgctgtcag tcatctttta aaaggtttac taaatgaaga tttgaaaagt
    35341 ttggacagca tttctgaaat gggtatttct gctttactgg aaatcagtaa cattctatca
    35401 ggttcataca ttaatgcttt atcacaatta ttaaattatt cgatttcttt agaagcacca
    35461 agaattgcag ttgatatggt tggagctatc ttaaattatc ctgcatcagt ttttggcatg
    35521 atgaaagacg agcttatgct tattgaagaa agtttttatt ctggagataa gtcatttaaa
    35581 agcaatctgc ttataatgcc cgaaccggct tctttggata gaatgtttga aaagttaggt
    35641 gctttatgac cggaaaacag attgttgtag ggatatcaga tttgaataca gcatattctc
    35701 ccgatgagtt gatttcctat gcattaggat catgtatagg aatatgcctg tatgataagt
    35761 tccagaaaat tgcaggactc tcacatatac tgctgcctga ctcttctaca tataaagcag
    35821 ttgataatat gaagtttgca gatactgcaa taaaagaact tattagcaga atgataagat
    35881 taggatgtag gaaaaataac ctaactgcga agatagcagg tggagctaat atgttttcct
    35941 ggtccgggga gacggtaggt gacaaaaata taaaatcagt tgaggaacaa ttgagaatac
    36001 atgggatacc attacttgct aaagacgtag ggggggatta tggaagaacc gtttcaatat
    36061 gcgctgaaac aggtgtagta accgttaaag cacttaccag aggggttaag accctgtaat
    36121 tcagtttttt atgataagca tatcctttat atcatcttca ttaataatag ctataataac
    36181 ctttaaaaca taaggaagaa gatctgccct gtcaggcaga tttacattta tatctaaatt
    36241 ttcataatac ttattacaga actcaatcat tctgcttacg gagaggtctg atacttctga
    36301 aatggtatca gctttaacga agatatcaaa aatcggaatt tttgcaatgt atacagcatt
    36361 atctacatat tcaatttcaa cataaatatt atacttggtt aggagttttt ccaagtggga
    36421 tgtattaatt aatgttaata aatcacgatt tcttttaatg aattctggat gactccttac
    36481 agcagagtca atggtttcac tccagttttt tcttacatca gtagaatttt tagctttcat
    36541 tagaacctcc gtctgcttta taagttcaat tttatacttg ctttatctga tagtaaaggc
    36601 gatagcaaaa aaaactataa tatggttgta catatcatag ttttgctttt ggacgaatgg
    36661 aagctatatg aagtaatcct tatcctttta tggtggcagt taaaatatct tactttatac
    36721 gaatttctta attgcctgca caagttttgc ttcttgaaaa ggctttacaa taaagtcttt
    36781 tgctccaatt tggattgatt ccattaccat atcctgctgc ccaagcgctg aacacattat
    36841 tatgttggct tttggatcca tctttttaat tgccttcaga gcatctactc cgctcatctc
    36901 aggcatggta atatccatta caaccagctt gggttgtagt tcctgatact tctcaactgc
    36961 aagcatccca ttagcggctt cacctgctaa aggaatatta ttccttgata aaatatcctt
    37021 aatcatcatt ctcataaatg ccgcatcatc tacaactaaa acaccctctg tcatatttac
    37081 ctccctctat atactattct tatcaagtat ttctgatatt ctcataccat aatactcatc
    37141 aattactaca acttcacctt tacctatagt cttattatta atgactatat ttacgtcttc
    37201 acccactagt ttatcaagct caattattgt tccgcttgta aattttaata tttcactaat
    37261 aggtttttta actcttccta tttctataga aacttccaaa ggaacatcca ttaagatatc
    37321 atatctgtta ccaaacatat taggtttctt attgtcctct ccaaatgtct gtaattctac
    37381 tttttgtata tttactttac ttggtggttc agcagtatta gacttactgg ctttaacctc
    37441 ctgctctgat tgaacaggtg tagataaaga ctctgtatac tgctgtttga ctttttctat
    37501 catttttttt gcaaaatcca aagtcatgac taacatgatt tcactcttta atgtatcttc
    37561 aacatcaaga tcgaaaatgg tcatgacaag agtttcaccc ttaatgaaat catctatatt
    37621 ttcagttgaa tagtctgtaa tcttaacatc tggagtagat atgttcagtc ttgtgttcat
    37681 catttctgat aacgcagttg aggaagctcc aatcatctga ttcataagtt ctccagcagc
    37741 acttaagttc atttcatcaa tagggccttc tttaacttct cctttgccac ccatcattat
    37801 atctgtaatt atcttgacgt cttcatctct cagatttaaa acattcttac cagatattcc
    37861 ttcaatatat gttatttcca ctgttacata agggattgca tattctgtag gcatattatt
    37921 atcttcaagt ataacaactt ttggagtcgt aatactgact tttctgttca gaatttttga
    37981 aagagcagtt gcagaattcc ccataccaat attgcctatc tcaccgagca tatctatgtc
    38041 ttcttcggta agaggactgg ggctacctcc atttatgagt ttatcaatct ctgcctgagt
    38101 taattctttt cccatttcat gatgtccttt ctatagtatt attgattctt agtgcaattt
    38161 tattattctt ctgtcctaaa tccacagaga attttggtat attatttaaa agtaactctg
    38221 cttgttctgt tgttttcttt tttagtttta ttacatcacc tgcctgaatt ctttccagtt
    38281 catctaatgt aagataagct ttacccagat tgcagatcat ttcaacaata ttgtttgtaa
    38341 ttttattctt caggaactct tcatgagaaa tgccatcagg ttttgatgtc cttgtaaatc
    38401 cttttgtgac agctatattt cttaatactg gttctataag gaaatatggt atacagaagt
    38461 ttataaagcc ttcattttcg gctatatgcg ctttcaatgt aactattgca ataatatcgc
    38521 ttggtgcggc gatttgtatg tgctgtccca tattttccac tctttgcaga ttaagcttta
    38581 tatctgcaaa gcctttccat acatctatca tcggctgtat aaaaatattc aacactcttt
    38641 ttattaaatt aatttcaatt tctgtatatc ctgagaatat attctgattg cagttaccat
    38701 ctccacctaa aattaagttg attatacttt caacagccga atatgataat tctacagatg
    38761 cattatgatc actttgatta actctgaaaa ttccaacaag agaggatttc tggactgagt
    38821 ttgtgtattc aaagaatgtc agttcctcta tgaaagtgac ttcaaagaaa caagtggttc
    38881 tgacaaaatc tgaaaccctt aaagataata acttggcaaa attgctaaat atatcttcaa
    38941 tagctcttac ctgttctctt gtgaatttat tcggcctgct aaagtcataa ggttttacct
    39001 tttctttttt gacaagaaca ggttcctcgc ttttagtgtt aaggctttga attaattcgt
    39061 ctatttcagc ttgtgataaa atatctttca ttttgtcctc tattgaataa tcagttctag
    39121 aaagaataca ttctgtatac tatctattcc caaggctgtc ataagtacat atttaagcct
    39181 ttctcttaac atctgctgaa tatcatcttt tagatattct tcctctgttg tgtttcttag
    39241 tacatttata attgtatctg taagaatata gttatgtctt tctatatatt ccaaatctgt
    39301 ttgatcagtt atctccagca taaaagatgc ctgcacgaaa ccattactat tcttcatatt
    39361 agttataaat gtatcttcaa ttacataggt atatataacc tcttcgattg gttctgtagg
    39421 tgacgcggtt aaggatggtt cctctggttt ataagcattg gtgtctggaa aggcaataag
    39481 aattaccagt gtcagtgcca ctgttagaaa aactgttgct actattataa ttacattttt
    39541 cattctttca cctctgcttt cataataact atatctgttc ttctatttaa cgcccgtcct
    39601 tcctcggtgt catttgatgc gatagggttg ttttccccat atccttttat ttctatcttt
    39661 tttgcaggga agaggtcttc ctgttgtatg aaataaaaaa cgtttagagc tctgtatata
    39721 gatagttcga aattatctct gtatctggca gtatttattg gaatattatc tgtattacct
    39781 tcaatgacca gtttgtctaa aacactttca tattctgcta taacgccaac tatctcatat
    39841 attatgtcgg cagcttctgt atctaaatca gctattcctg attcaaaaag tatcttgtta
    39901 ccaaaacgta atattattga ttcttcattc cttataatgt caacatccca gattccgctt
    39961 ttataggtta tcagtctcat taactctcca taaagctttc caaagtcctc atcaatagta
    40021 tttccaattt cttcgggagg aatcttggta tcttcaggat ctgaaggaat ctctgtgtca
    40081 ctgtttacca agggaatagg gttacctctt tcgactatcg aatctataag attagatttg
    40141 ttctgtgaag aatctttatc cttttttatc ggattgaatg gctgttgttc agtatgtata
    40201 cttacaccca ttctacctct taaggattcg acaatctgct gccacttgac cgcgtctatc
    40261 gttgaaaagg aaaaaagcat tacgaagaat gtcagaagta aagacattac atcaccatat
    40321 gaaactatcc agtcggcagc gccttccggc gggtcaggct tcttcctctt cacttgatgt
    40381 gctcttctcc tttccgctct taacaatata tgaaccgagc ttttctttta taagatacgg
    40441 agactctccc gcttgtattg agagaactcc ttcaagtatc atagttttac attcaacctc
    40501 attattgctt ataaacttta atctgcctga aacaggaata aaaaagatgt ttgccagcag
    40561 agcaccgtag agtgtggtta taagtgcaac agacatggaa gcagcaagtg ttccggtatc
    40621 ttcaagtttt ccaagcatgt tgataagtcc tataagagtt cctatcattc caaaagccgg
    40681 agcaagcttg cctccttgag tatatatatc ctgtattaac agatcctgtt gttctgttat
    40741 cgcgatatca ctttcaagag tattacgtgc atcttcagga cttgttccat ccacgattaa
    40801 aagtattgat ttcttgaaaa attcatcctt catttcatcg gtatattttt caagagcaag
    40861 caatccttct ttctttgatg cgtaggcaag ttccacaagt ttatcaagaa tatcttttct
    40921 gttctgaggt ttgaatttta ttaccttaat aaaagctttg actgaagata aaagattctt
    40981 aagagggtaa ttgcatacca ttgctccaat ggttccgcca ataacgatta gaatggacgc
    41041 tttatcatag aaagcactta actgcccatt ttccaatatg gcgaatatta ctaaaaaacc
    41101 cgcaagtgca atacctagta cagacattat cctaccttag cccttatttg tatttctgct
    41161 ttgtacatag ttactttttc aactacctgc aaagcagatt ccttgacaat atatttgtca
    41221 gcattaacca ttttaatgac agtatgggga ctctcctcaa tatggattat caaatcacaa
    41281 tttagaacta ttgctttatc atttaattgt gtgagtaata tcatggtttg tcccctttgt
    41341 cactatctct taagatttac taattcttca agcagggaat cggaaacaga gatgattctt
    41401 gagtttgctt gaaatcctct ttgggtaact atcatttcag tgaattctgt tgcaaggtca
    41461 acatttgaca tttcaagtga tgaggtagca acagaaccgc gatctcctgt gctggccatt
    41521 cctatattcg catcaccgga gttccatgtt gttgtgaaca tatttcctcc aactttctgc
    41581 aatccattgg gattaataaa tgaagctaca gctagagtag atatatgttc ctgaagtccg
    41641 ttggtgaact gtcctattac ctgtccatca ctgcttatac taagagtttc caattcacca
    41701 gagctgtaac catcctgatt agattttatt gaagtgctat tagaatactg tgtaaatccg
    41761 attgcatcaa atgatataga tcctgtaggg aatgttatac cttcagcacc attggagaaa
    41821 gttatctgaa cctcaggaat tgaagatgct cctgtgtcaa gtttacctga tgaggtaaaa
    41881 gatatagaac cgcctgtagc acctgcagtt atttccatct cgtcatcact ttccataagt
    41941 tcataagtat aatcagaagc agcggaagtt ctcatgaact tgaaagtcag agtgtgagat
    42001 tcaccaaggg agtcataaat agtcagatta tgaagtacgc cgttctcatc atttactgaa
    42061 tcatattcca gcgtctcact gcttaagttt cctgttatgg aaatttttgt actgcttttt
    42121 gcagacattt taatgtttga gaaatcaata ggttcagtgg gtttgctaat atcaatgttt
    42181 cctgtagctg gatttctgtt ccagcccata accttatatc ctcctccatt taccaaattg
    42241 ccgtctttat ctacattgaa gcttcctgct cttgtaaaat atgaacctgt accatcagta
    42301 acgacaaaga atccttctcc atcgatggca agatcatttg ggttgtcagt tgcctgaata
    42361 ctgcctttgg acatgtttgt agtaatattg cccaaactaa caccgagacc tatctgttga
    42421 gggtttgttc cgcctcggcc ttcttctgtc ggctgtgttg ctgtagcgac taattggctg
    42481 aacacttctt caaatacaac attgctgctt ttaaaagcag tagtgtttac atttgcgata
    42541 ttattaccta ttacgtccat ttttttctga tgtgacagca aacctgatac cccagaatac
    42601 attgatctta acatcttttt gctcctttca atttgctaag tgttttactt ataattattt
    42661 ttaataaaca taaacaacgt tatccatata tactgaaccg aattgtgtgt ttagtaatac
    42721 ttcatcatcc tgcaaggtga tgctttcaac taaaccttct atgtaatcca tcgtaggatt
    42781 atcttcattc ataatggaag ctattacatt ttttcctata tagtcaagag ccttcatatt
    42841 atcaattttc atactgagac cattgatgct ttccagcata gaaaattgtg ttatttgtgt
    42901 catatactca aaatcatcca ttgggtccat tgggttttga ttttgcatct gtgttaccag
    42961 cacttgaaga aaatcttcct ggttcatagt tccggcttcc tttgctttat tctcatttac
    43021 atattttgtg gtttcatcta ttggtactat gttcattatt gcaaatctcc ttaactaaat
    43081 gtatatattt accagttcgc ttcctgatat tattttgcca gtttcatttt cattaacggc
    43141 ctcattgttg attacatgaa ctggtttaag cttgtagtgc ggattttcag tattgccatg
    43201 gttattgttt gtaaaactat atcctgttcc tccataacca gacatgtcgt ttgctgactt
    43261 ttcaaatact tttatctgtt caactttgta tcctttttct gtcagcattg ccgttagttt
    43321 ttcagtttct gaaataagta ttttactgac ttcatctttc tgtgagataa gattgagttt
    43381 aatctgttcg ccatcgaaag tggtttcaat ttccatacct ccaaaaattt caggtgtaag
    43441 atggatttca atattcgtaa ttccgttttt acaggaaaga tctattttat caactatctg
    43501 atttagcaat tgatctttct gtatcggatt agtgctgttt tgattaatat tcacagttct
    43561 aatctcacct ttactaaata tattatcggc tgaaacggtg ttttcttcac ttttaaaagg
    43621 cacatattca ggtttgtttt tgggtttacc tggctcatta gaagtatctg aatctgtaaa
    43681 accttctgct aagtttgcat tttcagcatc aattttactt ataacattga tattctccat
    43741 tttttcaaga ctattttgtt ttaagcttat attactgtca gaatttaaaa gtattgattt
    43801 cttttctgta ttgaatttgt cgtaattaac caattcttgt gattcaaagt tatcaataaa
    43861 ttcatttaat tgcatattta tacttttgtt ttcgtttaac aaatctgatt caatggttaa
    43921 aggctttata ggagtttgtt caaaacctgt atttatttct ttattggaat aagagtgaga
    43981 gactatacct tttgatacat tttcaattgt gctgttattt ttttcaattt cagtattttt
    44041 caatgggata tcactatttt taacataact taagtttttc agtgaaaagc gattatttaa
    44101 atactcataa gaaactgggt tattatcatt ttctatattg aaatttacga tatccttgtt
    44161 gatagtatta ataaacgaag aattattgag atcaggtatc ttttcagaat aagctttgaa
    44221 aatagaggaa tagttttcaa tgaacttatc catgggaata tggttttgct gaacaaacaa
    44281 cagcaggtca aggtattcaa ttttttcaaa agtgtcttct tcagaatcgc taatttccat
    44341 acattcaagc agtttgccat ggatattatc atcttttaaa agcatgttgc tatctgattc
    44401 tgctgacttt gaaaacacag cattgaataa ctcaagaatg ccttgaaaat cctctatttt
    44461 tgcattgttt tgattgtttt tgcagacact gtttaattta tctgtttcaa tttcattatt
    44521 atttattatt accacattgc agccttcctt aattaagctt acttaatata tcggctgctt
    44581 ttgcggtttc cattagttct aatatagctg ctacgctatc tgttttcatt gtttttaata
    44641 ttttaactat ctgctgggtg tcttgaattg caccaagtat tttagcggca ttttttggtt
    44701 ccattgcttg gtatatttta cctaactctt caacttcaat aatgcctttt tcaaactgtt
    44761 caatttgttc agctaattcc tgctctcttt ttataagatc tgctctggtt tgttctaata
    44821 agctttctgc attttccagt ctggacattc tttcctcata atctgttttc ttcgcttcta
    44881 gctcttcata ctcaacaaca agcatttcgc gttcttcttt taaaatttcc gattcagctt
    44941 ttttcagttt ttgtgagtat tcaaattcat attgtgcaat tagtacttct ttttccttgc
    45001 ctaaaagttt tgttgttata tcatcacgaa aagctgttct ccatcctgtc caatcgaata
    45061 tgaatattaa aagaacagat gcaacaactg aaatgcctaa aattattatt atctgcttaa
    45121 caggtatttt tattttcttt ttagaagtat ctttatcatc attatcttta tcttgaatat
    45181 caggtacttc gtcattgtca gtatccttgt tttttttctt aaaaagtggg atacgggatt
    45241 ttttagaagg tttatcctca ttattcactg aatcattttc aattaaatca acatcatctt
    45301 ttttcttctt caccttacta ttccgccttt gctatatagt tgtagtttat aagttcctct
    45361 atctgctttt cttcaaattt cctgatttca tcaaggtaca gtgaatattt cttttctcgg
    45421 attttttcat aagcagagca atttattttt gcttttttta attcctcaag tctttgatcc
    45481 ttaatagtgg taagtttttc tatttctttg ttcatctgtt cgatttttac tgctaactct
    45541 tccatataga ttttgatttc tctgaaggtt atgctattgc aatcgtttaa taatttagct
    45601 tcattgtcct tcaacatctt tatatgcaaa tcttttaaat gttcaactgc ccttaaatta
    45661 tcgagtgctt ccttcaatct taatgaacac tcatctttta tattgtttgc attatcaagt
    45721 actgcctgaa gggaaaattt aaaagctttc attgtctctc tttctaaata atcagtgttt
    45781 caagtgaaga tatagtatct tcgtataatg cggtttcttt agtatcctgc cttaaaaaac
    45841 cattaatatt atctatgttc tttattgctt gatcaatctc cttattgctt ccttctttgt
    45901 atgctccgat agatattaga tctctgtaat tttcatatgt cgccattaga tttcgggtat
    45961 tcattgctgc attatagtgt tttttatcta taatatcagg cataaccctg cttatgctat
    46021 ttagcacatc aatagcggga taatgatttt tagaggctat agctcttgat aatactatat
    46081 gtccatcaag aatgcctctt acagtatctg aaatcggttc attcatttca tcaccttcaa
    46141 ccaaaacagt gtatattgct gttatacatg attgatcatt atatccggtt ctttccagta
    46201 aacggggaag aacagatagt actgaaggag tatatcctct tgctacaggc ggttctccgg
    46261 ctgctaaccc tacctcgcgc tgagccatgg caaatcttgt taaggagtcc atcataagaa
    46321 ggacattttt atttttatct ctaaaatatt cagcaattgc agtagccgct aatgcacatt
    46381 ttattcgtat tattgcgggt tgatctgaag ttgctaccaa aacgattgat tttttcagtc
    46441 cttcttttcc aaggtctttt tctataaaat cattaacttc tctgcctctt tctcctacaa
    46501 gtgctattac attaatatca gcagaggaat ttcttgcaat cattcccagt aatgtgcttt
    46561 tcccaacgcc tgtaccggaa aaaataccta gcctttgtcc tttaccgcat gtgataaggg
    46621 aatcaatagc ttttataccc aaaggtaaag gttcagaaat ccttttccta ttaaaagggt
    46681 taggggagtc tgcgtatgca tccctgtatt cataatctgt aatatcagaa gagtcaacgg
    46741 ggcgtcccag tccgtcaaga acttttccta caagcttatc gctgactttg acttttaact
    46801 gctgtcctgt tgcataaact ctgtctccat tgttaatgcc cttaatatca ctaaatggca
    46861 taagcaggat ttttttctca ttaaatccta cgacctctgc taatatgcta ttattaatat
    46921 catcattttt tttgcatatc atgcagattt caccaatttc tgctcttggt ccactgcttt
    46981 caatgcttga acctatgatt tttttaactt caccataagt tctgtagcag aaagtatttt
    47041 caacattatc aaaatactta tttagattga ttttttccat ctatttaaca ttcactaact
    47101 tccttgatac ttcattaagc tgagtaaata cgcttatatc aatatctccg gtttcagtct
    47161 cgacaatgca tgaagagttg tccagtcttg catcatgctt aatgttaagc atatatttct
    47221 caacttccca gtctggatag aatttttcta tgttatattt attaagtctt acgcatatta
    47281 cttcaaaatc gttcctcaat atatcttcta tagcattttc aaaatacttc ttaatatttt
    47341 cttcagaaag ggaaattttc tcgtgtagta tcttttgagc aatattgaca gccagatcaa
    47401 ttaactggct ttctgtatta tctattattt cctttttttc agttaaggtg ttatttagtg
    47461 cgtcatttaa gttgttcaat tgttcagcat actgtttttt tgcttcctct ataccttgtg
    47521 cgaatccatt actgaactgt tcttcttttt gggtttttgc ctgctttagg atgttgtcag
    47581 cttggatttt agcttcgcta agtatttttg cagcctcctg ctcagcatta cgaattatct
    47641 gatcagcaat ttggtatgat tcattgtcat cagaatcgtt tagctgatgt tcttcaaagc
    47701 tgtttaaact aattgcttct ggtttggatt cagtgaaact aatataatac ttactattca
    47761 tcaaaactac atctgaactt ttgtataaat cactcattta ttcaccttcc ttacttatat
    47821 tatcggttat gtttactgtt tctttagata tcttcctagt caattatttt gtcgtctcca
    47881 gtctttctta ttaatatttc gcccttttca tcaagtgccc ttatgacact aaccaaagct
    47941 tgttgcgctt tttctacttc gcttaatctt actggtccta aaagattcat ttcttctgtt
    48001 accatctgtt ttgcacgctt ggaaaggttc ctaagtataa gttccttaag ttcttcggtt
    48061 gcagttttaa gagcaacgga aaggtctttt gtatcaactt cggaaaggaa tctctgaatt
    48121 tccttgttgc caagattaat gatgtcttca aatacgaaca ttcttttctt gacttcattt
    48181 gcaagattat cgttttcttc ttccagcatt tcaaatatct gtttttcagt actcctgtca
    48241 actgcgttaa gaatattgac cagtacttcc acaccttcag tttttgtata tgtctgtatg
    48301 cccgatcttg taaacttttc tcccattact ttctcaatat cttttatgat atcgggagaa
    48361 attttatcca taagagcaat ttttacagaa atattggcct gtctttctct aggcagcatt
    48421 gccaatactg aggctgcctg gctgtttttc aagtaagata aaatgaaagc aattgtctga
    48481 tcattttcat actggatgaa attgaatact tcaacaggat caatatcttt catgaaatca
    48541 aaaggtttag atttttcaag atccatcatg tctgtaatta attccattgc tttttgaggt
    48601 ccgactgctt cataaagtac ttctcttgca taatcaacgc ctcctttgtc tatgtatgat
    48661 tttgccaaac aggagttata gaaatcctgg agtatcatca ttctttcttc tttgtcaatg
    48721 gtgttgtgat taactatctc gaaggtaatt gttttaatct catcttcgtt aaaatacttc
    48781 ataatttttg aagcctgacc tttgccaagc ataatcagca agtatgcagc tttttctttt
    48841 cctgatattt tgtaagtaga attcataata tcctgcatgt ctaatcctcg ctaatccact
    48901 ttttcaatat atcggctacg ccttctggat ttttatctac atatttttct atttcgctac
    48961 ggtaatcatc ttcaggattc tttttttggc cgagttgttc aaggtcaagg ctttcatatt
    49021 tagctctagt tgtagaagct gtctgatttt tagtttttgc caatcccttt ttgaggattg
    49081 taattatcat aagccccaaa acaaacattg taattatcat taccgattta agtataaagt
    49141 caggatctag taaaaagctt acatttgcag tgtttacaac tgcatttgaa agagcatcat
    49201 caacatcatt agatattgca aattcgactg cctgaacaat tatgtccgtt tcactgcctc
    49261 ctactgcatt tgcagccatt ttctgaatat ttgtaagcat atcatctgtt actcccgggc
    49321 tgtttatcat tatagaaagc tttatatcat ctattacacc attttcatgt tcaattgatg
    49381 tagtattctg attcacataa agctgagtaa ctgtttctga agaagtggct tcacttccaa
    49441 gaattgtatc gttatcgtta tcaagtgtcg cactttggct gtctataact gtttcttttt
    49501 tttctgttat tgaatagggt ataccgtctt catcgtttac cggagtaaac tcggttttct
    49561 gctcagatat tttgctgtag tccattttta tgcttgctgc aactttgacg ttgccaaatc
    49621 cgaaaaccgg ttcaataatg gatataacct tgttttctat ttcattttca agtgatttct
    49681 gaagttcata tttttctgaa gcggttccaa tttcgctatc tgattttctg tttaatacat
    49741 tcatctgagt atcaattatg gttatatttt catttgataa tcctgaaatg ctttttgcta
    49801 ctagatgttc aattgctttt acattagcag aagataatga atatcctgaa gcaacatcta
    49861 ttacaacagc tgctgttatt ggttttttct gactttcaag aacaaaaaga gtctcatcag
    49921 gcttacttat tgtaactacc gctccattta ctcccttcaa agtctttatg gtgttttgaa
    49981 gtctgtcctg aagttgaaat aatatatatg tctgtttttc tgattctgat attcccatac
    50041 tgacattgtc agtgtatatg tcataagata atcctgttct aggataaccc tcagttgcga
    50101 gggtcattct gaacttatct tcctgtgttt catttattaa gattgttgta ttgtttttaa
    50161 gtttgacatc tgcgccaaga tcagtcagct tagtgtaaat ttcaccagct tcataaacgt
    50221 ccaatccagt atacataaca gtatatgtag ttcttgtaaa aagtataagt agtacagata
    50281 aaccaattgc aaaaaggata cccatcagta taatgctgct ctttttcttt ttttcaagac
    50341 cagaccaaaa gcctttaatt tttgataaaa tagacttgat agattctgac attttttatc
    50401 cctctatacc tgcatctgca tcatttcttt atatgcatct actaatttat tacgaacttc
    50461 cattgtgaat ttgattgtca aatcggtttc tgctaatccg actatggctt tgcttatgct
    50521 tgcaatatcg cctgatgcta acatatttaa actgttttca gtatttattt cagcttccac
    50581 catatttttt atgagattgt cgaacacttc attgaattgt gtttttgatg tttggttcag
    50641 attgtttttt gaaatatccg ttaattgctg gatgttttgt atctgagata taccactaat
    50701 gctttgaata gccatttttg tccccctctt ttatggaata ttgaattaga gtatgtcagt
    50761 agctttggta atcatgcttt ttgttgtatt gaaagcgacc agattagcct cataagctct
    50821 gcttgccgaa agaaggtcca tcatttcctt tgtaacatcg acatttgatt ttgttacata
    50881 tccattctcg tcagcgaact cgtttgttgg atcataaaca agtttgaaag gtgtttcatc
    50941 ttccttaatg ctggaaatag caacacttaa tacttcctta ttttctttta catcttcaaa
    51001 tactgcacca aatagttttt tctgtactaa atcagcttct cttctttgat aaggctcatt
    51061 tgtatctgtc tgaatggtat cagcattagc aatattctga gcaatcaggt ccattttagt
    51121 ctgatatact gaaagggctt gtgaactgat gttgaatgct gcgatattag ccattgtcat
    51181 tacctcctgt cttctatagc atattccatg cctttaatac tttttgacaa cgcatttaca
    51241 agtgcgttgt actctaatac attttctaca atagaggtca tctcttttcc aatatcgact
    51301 gtatttccat tttccttagc ttctacatcg gttctttcag taatagaata tctgatgact
    51361 ccttctggcg taactggttc taaaatgttt aaaaatacat cttttgcttt ataccccgga
    51421 gtttcggaat tggctatatt tgatgatagc acctgattcc tttgtaagca cttatccatt
    51481 ccgtttgtaa tgacctgagt atttttgagt aaattaacat acatcatttg cctccaaatg
    51541 cttaaaaaag ttgtaatctc ttttatttaa tttatcttta tactttgttc cgaatccctt
    51601 caggcccttt taccaagtcg tttggtaaaa ccactatttg atactaggtg ataatatcaa
    51661 aattatatta tgaattataa tgcattgaaa ttgatatgtc aacattaaat gaaacaaaat
    51721 ctcaaataaa ataaaaatat tcttgttatt agtacatttt atcagtagta aaaatttaaa
    51781 attatataca gaaatataaa ctaacatatt tacgttagtt ttagtaaatt aattatgtaa
    51841 gtgcctgtat tttgttaatt tgttgacaaa ttaacaataa ttgttttatt gccttagata
    51901 agcaataaat agtctagatt cgcttacaaa ccctccgctt tccaatgttt tcctataaat
    51961 atcattactc aaaggacatg cgcagcgggg ggtgtagttc attttttctg cctcttttgt
    52021 tatgtgcaac aacatacttc tcccaacgct tatattcctt gattcctctc ttacgtatat
    52081 accaaagcta catattccct tttgtatgga atggggtatc ataactgcaa gaccgaggaa
    52141 tgatttatta tcgtcttcta aaataaatag ctggttcttt aaaattcttt cttcgtgacc
    52201 atctatgaaa ttctgtgtgt atttttttat tgatgaaata tcctctattt cagcttttct
    52261 gaaattttta gacgggtatt cagcttttcg aacaggtaga gaatttaatt taaagtaaat
    52321 atagtgtgtt ttaatcttac tgacatagtc cattaccaga cataaactaa tttcatcaaa
    52381 gcttgttaca agcataagtt caacgtcaaa catagataat ataagtttga agatctcctg
    52441 tccatgagaa agtagagaat ctggtatata aaatgaggtt aagtactttt cttcaagtat
    52501 agaaaagaat ccgatatgat tatcgagata gtttattgta taaaattcag aattctttat
    52561 atttatttca agataatcgt cgaaaggttt gccaagtctt tccaggaact tttcaaagaa
    52621 gttcttgtta gtcagataat tgcttttttt tagatcgtat aggctttttg cctgtttgtt
    52681 taa
//
LOCUS       DAOSXM010000008        48120 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2111
            NODE_461_length_48120_cov_10.804411, whole genome shotgun sequence.
ACCESSION   DAOSXM010000008 DAOSXM010000000
VERSION     DAOSXM010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864178
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 48120)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 48120)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:24:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,261
            CDSs (total)                      :: 2,229
            Genes (coding)                    :: 2,218
            CDSs (with protein)               :: 2,218
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 3 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48120
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_461_length_48120_cov_10.804411"
                     /isolate="AMR_MDS_2111"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            152..832
                     /locus_tag="PLQ68_02325"
     CDS             152..832
                     /locus_tag="PLQ68_02325"
                     /inference="COORDINATES: protein motif:HMM:NF024637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HXK71516.1"
                     /translation="MFCPKCGTRNDDNVKFCVNCGNPLSNQQQPNQYNQQQQQPGQFQ
                     QRPPLQQQGQFQQMPPLQQPGQYQQRPPLQNNQFAPMYQQPKKSKAGIIIAVIAVLVI
                     IGIVFVVWMNGGFTMSNVKQAYISTSLDPTTSEPLNKVKSISSTESTIYIAALIKNVD
                     DGANIVAVWYHLDSAESVVTDPLYINYDAWVNFSIDRIGSNFPTGSYVVELYVDDYLE
                     KTLEFSVY"
     gene            complement(874..1761)
                     /locus_tag="PLQ68_02330"
     CDS             complement(874..1761)
                     /locus_tag="PLQ68_02330"
                     /inference="COORDINATES: protein motif:HMM:NF014376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HXK71517.1"
                     /translation="MKIKTALKDLNMEISLGCIKVKIVSLVDVVTDSDWYVSNHSHTD
                     FEFHIIPSGQGTVEIEEVLVDLKSNDFYITGPYVKHTQRSSVKEPMREYCLECDITVD
                     EKCDDASYTKKEGKFLIESLSKIYQRTYSDADIVSLFEDIFEEDQTRSPGFYINIQLM
                     IMKIVTLIFRHLSQDDNMKYDYGYIKRKADDLRITKLLNYIKANYKNDILLKDVEKSL
                     FLSPRQINRIMIKRFNCTFHSYLYNYRIEKAKKLLKNSDMTIEEVAYDSGFSSHYYMY
                     QVFKKEGIGTPLDYRKSST"
     gene            1882..>3833
                     /locus_tag="PLQ68_02335"
     CDS             1882..>3833
                     /locus_tag="PLQ68_02335"
                     /inference="COORDINATES: protein motif:HMM:NF013256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HXK71518.1"
                     /translation="MENVNNLLKRLSDIKSQSYWADRIISQLEYAYESERLYKFGYLE
                     KINSAIRFVTQNNTEQGVITKNTVLSCEKSLMELSSHMKKFSLLCVSHAHIDMNWMWR
                     YDETVAITLDTFRTMLDLMDEYPDFTYSQSQASVYKIVEEHDPAMLEQIKKRVKEGRW
                     EVTASTWVEADKNMPSAESKAKHILYTKKYLSKLLDIPYDYFVLDYEPDTFGHSVNDP
                     EVLAKAGIRYYYHARGFNKLAAYRWQAPSGASVLVYNDPTHYNGKISTNPVLLVPSFT
                     YDTKHDTMLYVYGVGDHGGGPSRRDIERIKDMDKWPIWPNIRFSTYKEFFKSLEKAEN
                     NLPVIKDELNFIFTGCYSSQSPIKKGNKASEALLYEAESLSALASVKELSDYDSEKIQ
                     KAWEMVLFNQFHDIITGSGKVSTREYAMGEYQKVFATGNVLRSNVMRDISKNINLSDY
                     IIAKDDELMTVSEGAGVGYGIDDLQISKVSRGRGPTRVFTVFNPSSFERKENAVIDIW
                     NWDYDISSMEFFDGKKQKVSHQLISDKANRGWGGQYYYRVIIEACVPAFGYTTYILKS
                     NPETALTYKYQPKDRTIDFPKYTLENEHIKVCFDAVNATITSFIDKATSKEYVDANKP
                     TGVFRYIEENTLRKMTSWIVGRYMNI"
     assembly_gap    3834..3933
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3934..4534
                     /locus_tag="PLQ68_02340"
     CDS             <3934..4534
                     /locus_tag="PLQ68_02340"
                     /inference="COORDINATES: protein motif:HMM:NF036773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyl hydrolase-related protein"
                     /protein_id="HXK71519.1"
                     /translation="KLRKDADDDRPSTGFVMAINSENDKSLVMFSDSKYGFRTQDNSL
                     SLTLIRGSYDPDPYPEIGLHKFKFSIGLFDKNSLNRITKETSCIIHPLSSMSVKPQKG
                     HLPMQGSLLDIKETGSCIITAIKKSEYVNNGIVVRIFEAEGKNSSVALDFGKKIKKAE
                     YISIDEITGHDSKNLIITECCITILINPYCIETILIEFE"
     gene            complement(4625..6049)
                     /locus_tag="PLQ68_02345"
     CDS             complement(4625..6049)
                     /locus_tag="PLQ68_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019850246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HXK71520.1"
                     /translation="MKNKMAYKLSAYFSITLVLFLIVISFLFVMLFRSHIISFYKSDM
                     QKEASSIADSISEFLDESGGWSRQGGISAYTKVLEDVTTNDVWIVDENLNFLVSGRGR
                     VNQVTYSDLPSNAEVLVNDVFSGKTTFSEEFSGIIEEPTLTIGVPIIINGSVKGAVLL
                     HSSIDGMTDSISQGISIMAISALSALAIAIILGIILSISFTNPLKKMRNAAISLSEKD
                     YSAKTGVKQNDEIGQLASAIDQLSIQLDMASKESERLIQMKQDFMANISHELKTPVTV
                     IRGSAEALCDKIITEPEQIELYHKQILNDSIMLQRLVEDLLELSRLQNTDFSVNKEKV
                     NVCEVVSDAVHTARNLAKKKNIDVRFEMSESREIQGDYARLRQMFMVVLDNAIKFSNE
                     NGNVEVVCNRDFISIKDYGCGIKKEDMKYIFERFYKIDNDSNRTGTGLGLAIAWQIAL
                     RHDIEIEVVSEIGQGAEFKFIFKE"
     gene            complement(6039..6737)
                     /locus_tag="PLQ68_02350"
     CDS             complement(6039..6737)
                     /locus_tag="PLQ68_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019850247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HXK71521.1"
                     /translation="MTQILIADDNKQITSILEEYAKKEGYSVTVVYDGTQVLEKFKNS
                     NYDLLLLDVMMPKVDGFEVVREIRKTSDVPIIMITARGEDFEKIMGLDIGADDYIVKP
                     FSPQEVMARSRAVLRRLSKDSIKDNKSILNISNLVINIDDYTVSIDNNNILLTKKELE
                     LLWTLASSAGKVFTRDSLLNSLWGYDYYGDTRTVDTHVKRLRAKLNEYKHESWDVKTI
                     WAVGYKFEEIKNEE"
     gene            6929..7429
                     /locus_tag="PLQ68_02355"
     CDS             6929..7429
                     /locus_tag="PLQ68_02355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK71522.1"
                     /translation="MTKIKKIFIIGLVILSLSAISVTSFAATEYKNPAEVYSALTETP
                     VEDVLALRQTSGMTFGQLALEAGKSEDFKALVFEMKKQILADKVEAGVITQEKADEVL
                     AILEAHIADCDGTGFEGAKAGIGIGFGTGTMQKQGNSLSRGGQGGMQRGSGQRQNLRN
                     GSCLGN"
     gene            7743..10379
                     /locus_tag="PLQ68_02360"
     CDS             7743..10379
                     /locus_tag="PLQ68_02360"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HXK71523.1"
                     /translation="MDNTTLYRKKKIPIKRIVIYIYVIIVVVVLSIIAYSLFSGLSKT
                     ANKEAQRASFELNASIYNEIYNYIDFSEHINHVYGDLVSNNLINFSDKDTRETLIMSF
                     MNNHSLNISVFAYINENNQFYGGIRRDSDTLEIYTLDQTTNWQFSVQNVNDENKDIII
                     GSDLIDFKTENWYELAKQTNENIILPVYYNEHISMHTIRSVFPINDDNNVFQGVIYLE
                     INLDNMQQIIESLIEDDNYSAYIIDKNTNTVVISTNELYPLGYNINETDDFLEYQQYF
                     DTSDKSFSKGGFIQGSYHTLEEYKNDDINWVIMTSVPNRLFLEGFSDNVSIFSLLTVV
                     VVAISFLLLHIISDKLVRPLNNLVKAAEEISAGKLDIDIPITRNDEIGILSIAFNKMA
                     SRISSLFENLENKVEERTNSLNEAKEKLQIILDSTAEAIYGVDLDGICTFCNKSCLKI
                     LGYTNEDQLLGKRIHNMLFNPDDEKHSNCRVVDEMKKGLVIHEEDEEYLKADGTKIFV
                     EYHSYPQYKDSEVIGAVVTFLDVTEKRKSTEKIRYLSYHDVLTGLPNRRSYEEFIKKT
                     DKHKNIPYSVIFADINGLKMTNDIFGHSIGDDLIIKASEIIKKCAGKNNFVARIGGDE
                     FIVLLPKTDYEKASEITAKIKDMISETKIVAIMCSISLGCDTKYDDKTNLEQVVNSAE
                     NKMYRQKVMNRKTVNSNTINTIVSTLHERSPRELTHSNNVSSLAAKIAKAMNLSETEV
                     RKIEMAGYLHDIGKIVLNNDILKKDIYSDEEREEFHLHPVHGYRILNLFDDTLDIAEG
                     VYSHHEKWNGKGYPKGLKGSEIPLIARIIAVAEAYDALINSKTVKPISKKQALDNIKS
                     MAGIMLDPNIVNLFVDIMSDNV"
     gene            10415..11503
                     /locus_tag="PLQ68_02365"
     CDS             10415..11503
                     /locus_tag="PLQ68_02365"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HXK71524.1"
                     /translation="MNKNNSMSRFLTNNNRFKKDNPNYSRVYLLNAALIVLSIVCLFF
                     AVLNFLENDVPFVVLLDLIIMICAILTIANFHKTDNIKITSFLTTILLTFLIAIYFYF
                     VENRNFGLFWFSIYPPFTYLIFGRKKGRIYTLTFGSIILTYVILCYKSWEPAVFDYQS
                     LINIIAAAICLSLLIVFYENSRKAAEDAIIMKNKELEIMSITDRLTGLYNRHKLDIAL
                     NDFLSKSDNCNNIFSIIIADIDYFKSFNDTHGHLYGDKVLVDLANIIKATAGDKNIVG
                     RWGGEEFLIICPGLNNEGVKELALKMLDNISNYKTKDNKKVSMSFGIASCQADETIDT
                     LLQRADASLYCSKDKGRNMVDGKPICDS"
     gene            11568..12239
                     /locus_tag="PLQ68_02370"
     CDS             11568..12239
                     /locus_tag="PLQ68_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015044306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HXK71525.1"
                     /translation="MYKILIVEDDLVIASSLKKHIETWGYDSKYITDFKNVLDEFSTY
                     KPHLVLLDVSLPYFNGYHWCEMIRKISKTPIIFISSFSENMNIIMAINMGADDFISKP
                     FDLDVLTAKINALLRRTYDFKPNDECLIANGLILNTNNLSMEYKDTVYELTKNEFRIL
                     QVLMSSKGKTVNRDTLMSKLWETDSYIDDNTLTVNINRLRKKLEAVGLNNFIITKKGI
                     GYLVP"
     gene            12236..13228
                     /locus_tag="PLQ68_02375"
     CDS             12236..13228
                     /locus_tag="PLQ68_02375"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HXK71526.1"
                     /translation="MKLFFEYLKYHIKSIILFSSFSCIFLIVFILYSVEPQPALYASF
                     ICFVFGLMLAILSFIRFLSKHKSLSEMKNKITITKDDLPVPKNLIEQDYEDLITVLHD
                     EKTSIESKKANTIAELNDYYTMWVHQIKTPISAIKLLFQTNDIHDVEIEQQLFKIEQY
                     SEMALQYIRTESDVADFLIREYPLDAIIRQAVRKYSKSFITKKIALDYTPTQKTVLTD
                     EKWLLFVIEQILSNSLKYTDSGKISIYTKEDDKYTLVIEDTGIGIESNDLPMICEKGY
                     TGINGRYDKKSTGIGLYLSKKILSKLSHELAIESQPGSGTKVYISFSSEERISD"
     gene            13326..14090
                     /locus_tag="PLQ68_02380"
     CDS             13326..14090
                     /locus_tag="PLQ68_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020492915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXK71527.1"
                     /translation="MLLDVKNLKKIYTTRFGGHNVQALTNVTFSVEEGEFIAIMGESG
                     SGKTTLLNIIAALDKPTSGEVFLKDKSLIGISEKEISAFRRDNLGFVFQDFNLLDTFS
                     VKDNILLPLVLSGHTYEEMKNSLNTVVEKLGISDLLEKFPYEISGGQKQRTAIARAII
                     TNPKLLLADEPTGALDSKATESLLKLFKKINEDGQTVFMVTHSVKAASHASRILFLRD
                     GAVFHQIYRGSSSNEEMYQKISDTLTVIATGGGFNA"
     gene            14083..16077
                     /locus_tag="PLQ68_02385"
     CDS             14083..16077
                     /locus_tag="PLQ68_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012201333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HXK71528.1"
                     /translation="MRNCMYFKLAAINIKKNAKMYIPYIITSIITIMMFYIMIFLTHN
                     PGLVGMKGEHSLRTVLVMGIYVIGIFSVIFLFYTNSFLLKRRKNEIGLYNILGMEKKH
                     IARMLTIENIYTYLINMILGLFFGILFSRLMLLLLFKILKAGTGFVFNISMQGLYMSF
                     IFFTAISVLILLNNIRQIHLSKPVELLKGGNVGEKEPKTKWLLAVIGIITMGTGYYLA
                     ITVDNAISALNNFFIAVILVIIGTYCIFTAFSIAFLKILRKNKKYYYKAKHFTSVSGM
                     IYRMKQNAVGLANICILSTMVLVMISSTVCLYIGQDDVLKNRFPTNIEIQTIFANEEK
                     AKAVSKTVNQKILESGYEIDAYNEFRFKTYSLEFDNKNHVFHHSNDISLSLDKMYITY
                     MFPIESYNINTGSDIILNDNEVVIYSPDDIDLSDKFNINDKTFYVKNIVENPNFSIQN
                     NGSYMKLIYIFVSNEEVIKDIHKDFVEYFGEYCDLSYLIGFDVKEPEEKQIELMYSIR
                     DELRNTDEYKNLSFIMYATAEEKLDFYAIYGGLFFLGLFLGALFMMATVLIIYYKQIS
                     EGYEDKHRFHIMQQVGMSKHEIRKSIRSQVLTVFFLPLVTAIVHILFAFKMITKMLEV
                     LNLTNVSLFIICTIATVIIFAIFYVIVYSLTARTYYKIVE"
     gene            complement(16078..17304)
                     /locus_tag="PLQ68_02390"
     CDS             complement(16078..17304)
                     /locus_tag="PLQ68_02390"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HXK71529.1"
                     /translation="MPFELLEKQDKYLSYKDYFILGPTVAYCNNSDEMIMNFDLFNGS
                     DSNTGSLILRSLDNGLTWQEQHMIEKSYVYDFKNSWVKSGYGALYTDEVANVMVYFAN
                     DTYWEENKLASLWRMRTLYYRISFDNGHTWTDKKYIIQKGMSYDKTHFIKEVEFEKNM
                     AAMVSPLVVRSQDGTLLLSVQIQVLNDDGTLLNPTGMGFMKAGALKGTWNKDKLEYEW
                     DINQYVTVGLDKSVRGLYEPTFGLLDNNKVIMIMRNSNYRQSDKIIGTKFYSVSNDNG
                     YTWSEPKMLTFNDGSIMYSSSCIPKLLNHSNNKLYYIGVINEKNPEGNLPRYPLCIAE
                     VDKKSCKVIKDTVMVIDTKRSNHETMEKTSNVVDYSNHGVYEDKLTGNLVVLAPYRED
                     LTKYPCYLNRYVVKVG"
     gene            17408..19975
                     /locus_tag="PLQ68_02395"
     CDS             17408..19975
                     /locus_tag="PLQ68_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004463638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HXK71530.1"
                     /translation="MRAFERVNCGIKSMDSLLDNIRLGDNVVIQVSHLKDYSKIANLF
                     AMQSIKDGKSVNYIRFGSHEAVLEEQKGIKIYTPNPNSGFESFTMEVRRIIESNGYDA
                     FYIFDCLSDLQAIWSTDLMMGNFFCVTCPYLFQLNTVAYFPILRGHHDYSTIARIQNT
                     TQLLLDIYSQNNELYLHPIKVWNRYSPDMFLPHKLDDSEDFIPLVNSIDLNNYYSLIQ
                     KEQSKTPLLNIDSYERFFIEIKQKHKQGTISNDDIDRVIKSMMTHDEFIETLIKNHFS
                     CDDLFYIKERMIGTGSIGGKACGMLLARKIIHNDNINSEDFLEPHDSFYIGTDVFYSF
                     LVENNLWTLHIMQQKDEYYFKKADELKEAILNGRFPENIRSQFRRMLEYFGQIPIIVR
                     SSSFLEDGFKSAFAGKYDSVFCASKVDPIKRLERFEDAIRRVYASMMSTSVLEYRKKR
                     QMDKTDEQMAILVQRVSGTKFDNYYMPCVAGIGFSYSLYRWSAELNPNAGMLRLVAGL
                     GTKAVDRTGYDYPRLVNLDVPEKTTLVTDEEKHRYSQRFIDVIDLEENKFTEIDFEKL
                     LLSLPKWYTDLIFEHDRNAEANLRDSRIRRNVLYISCHNVVKNKKLMEIMSYILAKLQ
                     QVYSTPVDIEFTVNFSPEGNFQINLVQCRPLYIWKNQKSITIPEHKFSDILFKSHNTF
                     MGNKPHISVDTVIYVEARKYHELSYKKKSSVASAVGKINKYYKNSNKTVLLLTPGRIG
                     TSSNEFGLTVSFAEISNFKIICEYTDKKAGFFPEFSFGSHMFQDLVENEFFYIALDDN
                     ADNILNTDSFIKLNDITSRFSEDINDYECIRIFENDLKTKIDLYADFDKRICVLLRR"
     gene            complement(19964..20713)
                     /locus_tag="PLQ68_02400"
     CDS             complement(19964..20713)
                     /locus_tag="PLQ68_02400"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HXK71531.1"
                     /translation="MANYRTSQIAKITGIHSNTVRLYEDLGLVSPAKRLVNGYRVFND
                     KHVLEYKLARHALSMNKQHKYIRKKAIQSVKACADSDLDTSLLYINELISYIRQEKIN
                     VEEIKKAKDAVITSKSGQENQLYFTRKKASEYINTTPDSIRNWEKKNLIQIKRKLNGY
                     RIYTDEDLEKLRIIKVLKGLGYQLDKIKFRIDMLNNDFEDDYIYYNNQSKEYIKILNE
                     CEKYLNSINIDEETANRMYKIVWCFKNSSSS"
     gene            complement(20978..22108)
                     /gene="nspC"
                     /locus_tag="PLQ68_02405"
     CDS             complement(20978..22108)
                     /gene="nspC"
                     /locus_tag="PLQ68_02405"
                     /EC_number="4.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003529734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0008216 - spermidine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxynorspermidine decarboxylase"
                     /protein_id="HXK71532.1"
                     /translation="MEYDFSNINTPAYVVDKSKLISNLEILRYVEKTSGCKILLAQKA
                     FSMFEVYPLIAEYISGTASSGLYEAKLGYEYMKKQNHVFSPAYKEEEFDEILEICDHI
                     IFNSFSQWEKFKDKALKSNRQFGMRINPEHSTQNHAIYDPCSAFSRLGVTKKNFKEDL
                     LDGITGFHFHTLCEQNSDALIETINAVEEKFGPYIKKMKWVNFGGGHHITRKDYDVLA
                     LIECIKAFKEKYNVEVYLEPGEAIALDAGVLVSKVIDIIHNEMDIAIMDTSAACHMPD
                     VLEMPYRPNVLNAKMPNELKYTFRLAGPTCLAGDVISDYSFDYKLKTGDIVVFCDMAI
                     YTMVKNNTFNGMPLPSIYLAQNSTDFKLVKKFGYEDFKNRLS"
     gene            complement(22109..23308)
                     /locus_tag="PLQ68_02410"
     CDS             complement(22109..23308)
                     /locus_tag="PLQ68_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009063003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase family protein"
                     /protein_id="HXK71533.1"
                     /translation="MSKVLIIGCGGVANVAIRKCCQNYEVFPEIMIASRTKSKCDDLK
                     KKLENEKTIIHTAKVDADNVKELVALINSFKPDTVLNLALPYQDLTIMDACLATKTNY
                     VDTANYEPVDTAKFEYKWQWEYNERFTEAGITALLGSGFDPGVTSVFSAYAQKHYFDE
                     INYIDILDANAGDHGYPFATNFNPEINIREVTAKGSYFENGDMIETEPMQIKRVYDFP
                     QIGPKDMYLLHHEEIESLAKYIKGVKRIRFFMTFGLSYLTHLKCLENVGMTSIEPIDY
                     NGMKIVPLQFLKALLPDPASLGPRTKGKTNIGCIFRGIKDNKEKILYIYNVCDHEEAY
                     KEVESQAISYTTGVPAMIGTMLVLNGQWRKPGVFNMEQFDPDPFMEALNKWGLPWQLD
                     FNPKLVE"
     gene            complement(23301..24161)
                     /gene="speB"
                     /locus_tag="PLQ68_02415"
     CDS             complement(23301..24161)
                     /gene="speB"
                     /locus_tag="PLQ68_02415"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014354937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HXK71534.1"
                     /translation="MSNNANGFMGCTSSYEEAKIVIYGAPFDNTTSYRPGTRFGSSAI
                     RSESFGIEDYSVYQDLSLSDFKIHDAFDIELPFGDTKKALYMIEEMSRSVVGDSKIPV
                     LLGGEHLVSYAAIKPLVEKYQDLVIVHFDAHADLRDDYLGQKLSHATVMRRIWEITGD
                     DRIYQFGIRSGEKSEFEFADKHTNMYKFDLDSVKDVKEKLKGKHVYLSLDLDVLDPAF
                     FSGTGTPEPAGVSFKELLNAVLELKGLEVVGCDIVELSPHYDHSGASTAAACKITREI
                     LIMIGGNCNE"
     gene            complement(24151..25002)
                     /gene="speE"
                     /locus_tag="PLQ68_02420"
     CDS             complement(24151..25002)
                     /gene="speE"
                     /locus_tag="PLQ68_02420"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004610779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HXK71535.1"
                     /translation="MEFWYSEKHTENVYLSIKVDKQLFSKESRFQRIDVFESLEFGKF
                     LALDGYMMLTEKDEFIYHEMITHVPMAVKPDTTDVLVIGAGDGGVLRELIKYPTIKRI
                     DVSEIDEEVVAVCKEFLPNTACGFEDKRVNIYYQDGMKFIRRHIEEYDLIIVDSTDPF
                     GPGEGLFTKEFYGNCFKALKEDGIMVNQHESPFYEEDAAAMKRAHKRIFDSFPLSKVY
                     QAFIPTYPSGHWLFGFASKKLHPVHDMNPQKWEALGIKTRYYNTNLHKGSFYLPNYVE
                     EMLENVE"
     gene            complement(25003..26460)
                     /locus_tag="PLQ68_02425"
     CDS             complement(25003..26460)
                     /locus_tag="PLQ68_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HXK71536.1"
                     /translation="MILLDQNRAPIYEALEELKTLHIVPFDVPGHKRGKGNKDLTDFL
                     GQKCLSVDINSMKHLDNICHPTGVIKEAEDLAAQAFGAKYAYFMVNGTTSAVQSMVLY
                     SLKRGDKIIMPRNVHRSVINAFVLCGAIPVYINPQINSELGISLGMSYNDVVKAIEQN
                     PDAKAVFVNNPTYYGICSNLKQITQLAHKHNMLVMVDEAHGTHFYFNDNLPISAMAAG
                     ADMASVSMHKSGGSLTQSSLLLCGDNINVDYMRQVINLTQTTSGSYLLMSSLDISRRR
                     LFLHGKEIFDTVIELSQYAREEINDIGDYYAYSKELVNNDTVFDFDVTKLSINTSGVG
                     LAGIEVFDLLRDEYDIQIEFGDLENILAYISVGDSDKNIEQLIGSLSEIRRRYKKPRT
                     SALKHEYINPVVEMSPQEAFYAKKVSMDIKSTAGHICGEFVMAYPPGIPILAPGERIT
                     EQIIDYIVYAKEKGSLITGPEDMTIKNLNVLIGGR"
     gene            complement(26471..27232)
                     /gene="speD"
                     /locus_tag="PLQ68_02430"
     CDS             complement(26471..27232)
                     /gene="speD"
                     /locus_tag="PLQ68_02430"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003503353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HXK71537.1"
                     /translation="MNEKMKLYGFNNLTKTLSFNIYDVCYAKSEREQRDYIAYINEQY
                     NSERLTNILTTVTQMIGATVLNIAKQDYEPQGASATFLIAEEAMIPHKNKEIVAHLDK
                     SHVTVHTYPEYHPDTSIATFRVDIDVATCGEITPLSTLDFLIGNFDSDIITMDYKVRG
                     FTRDITGRKLFMDHDITSIQDYIDAKTLERYDAVDINVYQANIFHSKMMIKEVDLQNY
                     LFNTDVYEIAPKTRLSITNSLRREMIEIFSGINVY"
     gene            complement(28160..29575)
                     /locus_tag="PLQ68_02435"
     CDS             complement(28160..29575)
                     /locus_tag="PLQ68_02435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK71538.1"
                     /translation="MRAVIVDIRKNTAAMLSDDGSIIKVRNRNYSIGQEVDAGMTTKI
                     MSIKATIALAAASLLFSIGLGTSSYYLPTKYVSMDINPSVEYSVNMFNRVIDAEGVNE
                     DGIRLLEQLNIKDLKNKRIEEALNMTIEEAVVEGYLSGEDAGVMISTAAQNSNNAIEL
                     AAKLQESVKTAVEKNNSSAIVYGEAVGLERVEEARELGVTPGKLVLVEKLIESSDDPE
                     NINKQEWLEMSVKDIVAKTNENKGNNKPEDTPGKPEETPGQDNKPEDTPGKPEETPGQ
                     ENKPEDTPGKPEETPEQENKPEDTPGKPEETPGQDNKPEDTPGKPEETPGQENKPEDT
                     PGKPEETPEQENKPEDTPGGPEETPGQENKPEDTPGKPEETPGQENKPEDTTGKPEET
                     PGQENKPDDTPGKPEETPGQENKPEETPDADDLEDEQEIEETEEEIESEDDEDMSDVG
                     KTDNTPGRPEETPGQGKSGRG"
     gene            complement(29581..30297)
                     /locus_tag="PLQ68_02440"
     CDS             complement(29581..30297)
                     /locus_tag="PLQ68_02440"
                     /inference="COORDINATES: protein motif:HMM:NF016430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HXK71539.1"
                     /translation="MNTIDDLALQAAKDEKVFEELLIKNKGFIIKCAYEVTKKFISEH
                     DDEWSVSIIAFSDAVKTYEQEKGSFYAYSKLLITRKLIDYYRTEKKYNNEISVDPSVY
                     DIEPEEDDENLQIKSQLSEKLAVSRDESLKYEISAANEEFAKFGFSFYSLAESSPKSS
                     KTKKACALVVNYILDNPEIYNEINEKRQLPIKKILMHIQVPRKILERHRMYIIAATKL
                     LSDDYPYLAEYVSYIRNERR"
     gene            30691..31176
                     /locus_tag="PLQ68_02445"
     CDS             30691..31176
                     /locus_tag="PLQ68_02445"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXK71540.1"
                     /translation="MVELRKITWENFNDCLSLDVTDDQKNFIDSNVYALAQAYIAATN
                     DKFPPIAYAIYNDQTVIGLVILVHKYAKDSKYYHENCYEVVSFMIGKQFQGKGYGKEA
                     FARVIDIIKTFPEGKAKSVCLSYDPMNETARRLYASYGFIETGSVNDIGEVVAKLMIN
                     K"
     gene            31287..31619
                     /locus_tag="PLQ68_02450"
     CDS             31287..31619
                     /locus_tag="PLQ68_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002565263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HXK71541.1"
                     /translation="MKKKYTHETAIFKALSDENRLEIIELLSNGEKCGCVLLEKLDIQ
                     QPTLSHHMKILCETGLVNRYKAGKWIHYSLSESAAETIKRLARLFDVAEKKNEHSNMC
                     KECDTEKT"
     gene            31630..33669
                     /locus_tag="PLQ68_02455"
     CDS             31630..33669
                     /locus_tag="PLQ68_02455"
                     /inference="COORDINATES: protein motif:HMM:NF017280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HXK71542.1"
                     /translation="MAQYRILKDAKRAAKQLEEEIISFGKEILTKSMPDLDKIKSLHE
                     WEIEKDRIKSVIRNAFPSVLFNRDDLNVKHVSSHEFKNFKVENVLFNSILGWQVNASV
                     YIPKGEGPFPAVICPTGHSSKFKENYYIPAQIFASNGYIAVSFCPPGCVGEHAYRNDH
                     FVNGYSGWLNGMWSQTYFIADALACVDYVYSLDYCDTSNKVSITGVSGGGTTSMYTAI
                     LDDRISFLAPVCCVSDNINAQFKDLYTACPETFGKGLARHGIETTYILALFSPKRLFV
                     VGGEQDEVFDYKVTQKVVDKLKYIYSLYGKDSNINSYFEKGTGHKYSQTMALEVLKQM
                     NVIQKRTDIPILLNRDEIVHLEREQLSCYPSAEVNMHTINRDIALQLKKDRPELNKEE
                     TVQVIKKILDIENMQTMAEHTYMFPLKNVPSRWCHKFRDVVLTHSNGKKIPLIHAFRD
                     DYVKRPVFLWFGETDTWEPFNRKGPLTKIIGFLERTPVKNEHSVMSVNISGLGELDMQ
                     HTTYDAASWNRIERILTYFSVFNDRPIMAYRIRDILIAINHAKKFTNEISIGGYGIGA
                     ISAIFAAFIGNEDISKVIAVSPIGSYEMIATEPSFNWPESIMMPDILKYTDLPSVLGH
                     LGRKAVCINPKDAYMNKLESTSSNEIFDNSIANGAKILYDDDWENALVKEICSDN"
     gene            33695..34252
                     /locus_tag="PLQ68_02460"
     CDS             33695..34252
                     /locus_tag="PLQ68_02460"
                     /inference="COORDINATES: protein motif:HMM:NF024135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HXK71543.1"
                     /translation="MIKYIVYKTKTDFTKRYAALLSAKFKIPYLSMEEAKKKLISGES
                     ILYMAGIRASKLYDIKKALAKYNVEAVVAVGMSAPSDEYNNEIKNSNNIKDIPLFYLR
                     GGTAPDKLTGFDKFLFNAIAKMTEKSKQKADKTADLSKKEEIQLTESFFKGIDYVAVE
                     NLDSIIDWYSSYLIIQDMISKKHKK"
     gene            complement(34278..36026)
                     /locus_tag="PLQ68_02465"
     CDS             complement(34278..36026)
                     /locus_tag="PLQ68_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006441543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HXK71544.1"
                     /translation="MAHKYSGFEPKWVREEFSKDSYRSVFKWGDPKRIKPPNENLFKL
                     MKQIFHIDDSYFHEYKEDLGFDKVRYDIPITLEEKHISVFTEIVGEGFVKTDDYSRLS
                     VAYGKTMYDILRLRNKIVENVPDAVIYPDTREQIEKIVRYASENKIPVYVYGGGSSVT
                     RGVECMKGGISLDMRLRFNKVVCFNETDQTITVEAGMSGPKLEQMLNDAVKLFNAKRA
                     YTCGHFPQSFEYSSVGGWTVTRGAGQNSTYYGTISDIVIHQAYATPAGVIETDKYPRK
                     AVGPDLGQIMMGSEGAFGVLTHVTLRVFRYMPETIKRFSYMFSDWETAQNAAREIMQS
                     EAGYPSVFRLSDPEETQVMLRMYGVIESPLKHLFGLKGFKEGQMCLFLGFTNGEKGFS
                     RNCAKNVRKVARKYNGMNLTGQVTKEWEKGRFNDPYLRDTLQDFGIVTDTMECSVNWS
                     NMSYVHEQVRKYCKSRPDTVCMTHMSHVYPQGANLYFIFITKMSDPEEFKQYHAGILD
                     AIQKSNASMSHHHGIGKMFGPWLEGSIGKNEYAVFRALKEHFDKDNIMNPGGTLGFDI
                     PKEQLRFLKEDNKINL"
     gene            complement(36016..37569)
                     /locus_tag="PLQ68_02470"
     CDS             complement(36016..37569)
                     /locus_tag="PLQ68_02470"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012201393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HXK71545.1"
                     /translation="MNRPLVLAFDIGTQSTRVMLIDQFGNIINKSQKSYEQAYYSTNP
                     GWAEQKPNVYWDTMALTCNELKSSSKEHWEDIIAVTITTIRDTSICLDSSCNPLRDVI
                     LWLDKRKATCNKKLPLLNRIIFKVIGMSEAVQLQREVSYCNWICEHQQDIWEKTYKYV
                     LISAYLNYLLTGNLADSKASSIGHIPFDNKRGNWMKKNDITRCIFEVKDELMCDLVDP
                     GDILGNITKKASDYLGIKEGLPVIATGSDKGCETLGLSVISEHQAAISFGTTATIQFM
                     TKKYMEPLPFLPSYGAVLRGYYNPEIQIYRGYWLVSWFKDQFCDKEKKQALESGVIAE
                     DLLNEKMMDIPIGCDGLLCNPYFTAGVSMPVAKGAIVGFSDMTTKMHIYRSIIEGINF
                     ALFEGKRTMEKRGRKNIKSIYVAGGGSKSPQICQITADMFGLPVYRIQTNEAGALGSA
                     IVAFTSKKIFKDINEAIESMVHIKHVFMPDMDKHRKYLKIFETQFVKIFGKLLPLYKK
                     SIQGGHYGA"
     gene            complement(37562..37900)
                     /locus_tag="PLQ68_02475"
     CDS             complement(37562..37900)
                     /locus_tag="PLQ68_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012201394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1667 domain-containing protein"
                     /protein_id="HXK71546.1"
                     /translation="MTVKKNNEEITVEGNACKRGKEFAILEMTDPKRSLTSTVKTVFK
                     NCPVLPVRTDYDLPKDMIGKAMEEINKIVVTKKVKMHDIIIPDILATGCNIIASCDLS
                     NSLKEGDNNE"
     gene            complement(37949..39199)
                     /locus_tag="PLQ68_02480"
     CDS             complement(37949..39199)
                     /locus_tag="PLQ68_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006441545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HXK71547.1"
                     /translation="MKTYDVTVIGAGPAGLGAAKSAYENGAKTLLIEREARLGGILKQ
                     CIHDGFGLLIFKEKLTGPEYAQRYIDMINKTSIDISLLTYVTDVKKNQDGFCHITMIT
                     QKGIKHILSKTIILATGCRERTAKQIFIHGTRPSGVFTAGTAQYYTNILGHVPTKKCV
                     ILGSGDIGMIMARRLTLEGAQVEGVYEVKSELGGLMRNYSQCLVDYNIPLYTNKTVTR
                     VFGEKRLTAVEISSVNDDMSVIEGTQKIVDCDALILSVGLIPENELAEKLEVPIDEFT
                     KGPIADQCNQTLKDGIFICGNAHHVNDLVDYVSKAGTTAGRCAANYINKERKLVKLQK
                     SDLLSYCVPQYIDKLSEQKNVDVYFRSKARIKDVRISVTADNRVIATKKLVEIRPAEI
                     ETIQLDLSKAGNDILNIFLSCEKV"
     gene            complement(39213..40922)
                     /locus_tag="PLQ68_02485"
     CDS             complement(39213..40922)
                     /locus_tag="PLQ68_02485"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HXK71548.1"
                     /translation="MGLIRRLNKKLNKMFNGSVHAREENGCVKLTGSLDCWEDIVKAG
                     YTAVNKNKFIGVLNDIEYTKQDIPQMRMPSVNDLKYDKIHTDVAVIGAGIIGSAIARE
                     LTRYDIKVMLIDKEHDVGMHASSRNDGEIHPGIDLLKGQVKQKYNSRGNAMYDQICKD
                     LDVRFSRPGQYLCFNKKYYKLIFSIARLYWKAMGIPTEYMTADKLRKKIPGISNAING
                     GVYFPTAGIVSPYELVIAYAENAVDNNAVIALDTAVTGMEISSNKIISLKTNRGTIYP
                     KVVINAAGVYSDKIASMANDRFFTIHARKGTNAIFDKKIVKNNNFIVSVYGMSSVKTT
                     HSKGGGILTTADGNLLIGPDAFETFDREDFSTEDKNINDILEKHRTTIPGIKKSDVIA
                     YYSGIRAATYEEDFVISKGKRTKNIIHAAGIQSPGLTAAPAIALDIAKMTKDILSEDI
                     KIKENKDFNPIRKAILDIKNMNEDEKNILIGQNPDFGIVICRCEEITLGQIKQAINRS
                     VPCDTVDGVKRRVRAGMGRCQGGFCGPLVLQTIAKEKNIALKEVTKKDNESLILYESR
                     KGV"
     gene            complement(40975..43950)
                     /locus_tag="PLQ68_02490"
     CDS             complement(40975..43950)
                     /locus_tag="PLQ68_02490"
                     /inference="COORDINATES: protein motif:HMM:NF028199.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HXK71549.1"
                     /translation="MAKPHLFVNEVRISKIREYLEKYPWYKKSFTRIQKMCDEMIEKG
                     FSVPDQKGYVFNDACRAHNVSLVFDPYDKDNYICPVCKVNFKDEPYRKAWISFYHSWL
                     SQMSVYLGISYIVTSDNKYANAIKEILEKYILYYPHYPNEDNEIGTTKIFQSTFMESI
                     WLCYICAGYDMVNDSTAFSDELRKKCLNLFKESAVVIKDYDESWNNRQAFNNSGMCAV
                     AFLTNDDDLLNYSLYGKHGFAAHIGHSILEDGLWYEGDNYHFATVPAMVNIATMCMYN
                     GIDMFNKSFNKRSLKDMFYGPLKSLHPDGTFPSRKDSPYANSIVNRWYCGLYELAYGV
                     YKDKSLADFLNFTYDMKPDKSMPLSSAAGIMDIMKPAYGTREELDWRGFLSIEPETHS
                     DKGLPVTDSINMTGTGLAVLRRKNSYLNLDYGHYGGGHGHPDRLNITCFLHGKRWFTD
                     YGTGNYYLDHLRYYRSTLGHNTINQDGKRQSAVSGKCDLFYKGKLFDAASAIIDEIYP
                     NTIARRTAILFDNDMVFDYVLIESDQNHVYHNVYHSFGKLNDEDYTSFEDIFEEEGYD
                     FLHDVKTIMVKDGYNNKFTDGPSSMSMYASADLEMVQYSAYAYGPPKSIPKLFPLLIQ
                     QKEGKKVIFANVYEHIDEKDIKSKNIVSFNTINDHKYLVTFSDSSVMEIDISEGVKIS
                     YDGNTESAVFKKENEEDSEISYECWIDYNSIDDKPLCIGLRNSNSYEKTALLFSGKVT
                     LSPYEYKSFRYPLKGKGISIQDGYLKVVYNKDKEPFVKKIIDVFKYDRSKPINEADHK
                     KNIFINHSSNFSRAERHWQGKDDLSVEGSIFTSSEQSLSIMLHIKDNEVLFSGGKYDY
                     DNDCVQIYIDRRDQCYRNVDTLTDGIYSMILKGGVNGNVSKIIPMTKCLKDVSAISLS
                     MNKTIQGYDVCIDIPFHCIGGIPSVGDIWGFDVVLSDRDSGVRRELIAYWSGALVGER
                     TYMCGETDHDPRRYGLLRF"
     gene            44056..45444
                     /locus_tag="PLQ68_02495"
     CDS             44056..45444
                     /locus_tag="PLQ68_02495"
                     /inference="COORDINATES: protein motif:HMM:NF025644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHHW family protein"
                     /protein_id="HXK71550.1"
                     /translation="MKKFRLDALIFVVFIALFGIMNLLNPDKPTISLQENRKLTEKPI
                     FTVESLFNKSYFTNWNNYYSDTILYRESLIQLNVNAKKNFGLGKDQISIVVVGNQPAP
                     TKSPKPTSTPTPVESAVPTDINSPVPTDNIPEDTNTSSPSPVPTGTNEPIEEEPTGYG
                     EATGMYITVGDETFKLDSYSEEAYDNYANYINKIKQLAGEDIEVYSMLAPSRSTYLAL
                     TKYDFLSSRAANVEQINSKIDSSVKNVNVLKTIVEHVDEYIYYRTDHHWTHLGAYYGY
                     TELMKTMGLEKEIVPLEEYDEKLVVEGYLGFYNKPYLTQADYDNPDTIIAYYPTVDYT
                     YTYHSAQYGTVEKRLINKERATPEKDYYLLFSDGGFGTYSVITTSADTDKSCMVIKDS
                     FGDSLISFLLPHYKTIYVLDSRTYNKEYCDNLNIVDFAKSKEVDDIIVNYWITNITYL
                     DDFMAKAYSTLD"
     gene            complement(45447..46319)
                     /locus_tag="PLQ68_02500"
     CDS             complement(45447..46319)
                     /locus_tag="PLQ68_02500"
                     /inference="COORDINATES: protein motif:HMM:NF018649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HXK71551.1"
                     /translation="MKIKENRLIGFAIIALIYVIACIFGIWVYRLTPGSVYIKVLTAD
                     MAATITVYLFSLLLKNSTVYDPYWSVAPIVILPLLAMEYANTGLGTIFMLTAVTTWGV
                     RLTYNWVKTFKNLNIQDWRYDMLKERSKGLFPLVNFFGIQVFPTVIVYLCMLPAIALI
                     QNDSFNILTLAGFVVCFFAVLLQGISDYEMHEFRKNNSDRCALMRQGLWKYCRHPNYF
                     GEILMWWGIFIMMMSSVPDMFYLLAGPLANTLMFVFISIPMAEKRLAGYKTDFDEYIK
                     QTRVLIPLPKPLFK"
     assembly_gap    46385..46484
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(46567..47088)
                     /locus_tag="PLQ68_02505"
     CDS             complement(46567..47088)
                     /locus_tag="PLQ68_02505"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HXK71552.1"
                     /translation="MHDYNLIRSKRRTISLRISQDCDVIVRAPLFASKKMIDGFVSKH
                     EKWIDKHIEIQKKQPDLKSLSKKEILELKKQAEEVLTRKTEYYSKILRLYPKYVKITS
                     AKKRFGSCTADNGICYSYRLMLYPDKAIDYVVVHELCHLKHRNHGKSFYSLVAKYMPD
                     YLERDKLLKGLQQ"
     gene            complement(47134..47358)
                     /locus_tag="PLQ68_02510"
     CDS             complement(47134..47358)
                     /locus_tag="PLQ68_02510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK71553.1"
                     /translation="MDQFFPLNKNVKKKNISSLIIAILLYVVVSIVMGVLQKLLGPIP
                     VLGWVMGLLGWLVWVYSLIGVILAIVKFLK"
     gene            47496..48077
                     /locus_tag="PLQ68_02515"
     CDS             47496..48077
                     /locus_tag="PLQ68_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010077557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
                     /protein_id="HXK71554.1"
                     /translation="MTLWELLLIAVGLSMDAFAVAICKGLSLKKITWKEALTVGLYFG
                     GFQAAMPIIGYFIGTGFSSFIQKWDHWIAFILLAIIGFKMIIESLKKSCDINEDTDSS
                     KSLSLKRMLTLAIGTSIDALAVGVTFAFLSVNIIPAVSFIGIITLILSFVGVYIGNYF
                     GCKFKSKAELAGGIILVLMGTKILLNHLEIISF"
BASE COUNT        15604 a         8577 c         7483 g        16256 t
ORIGIN      
        1 ttatatacgg tatctgctcc taaagattat ggtgctaact gtcaaagacg ggattgttct
       61 atttcatggt aaaattattc taaaggatat atttaaacgg tgaaaaacac tagttgccgt
      121 attctttaat caaaacaagg gggtagaaag aatgttttgt cctaaatgcg gtacacgtaa
      181 tgatgataat gtaaagtttt gtgtaaattg tggcaatcca ttatcaaatc aacaacaacc
      241 aaaccagtat aatcaacaac agcaacaacc tgggcaattc cagcaacgtc cgccacttca
      301 acagcaaggt cagttccagc agatgcctcc cctgcaacag ccagggcagt atcagcaaag
      361 accacctctg caaaacaacc agtttgcacc aatgtatcag caaccaaaaa aaagtaaagc
      421 aggaattatt attgcagtta ttgcagtatt agtaattatc ggaatcgttt ttgtagtctg
      481 gatgaacggt ggtttcacaa tgtccaatgt aaaacaggca tacatatcaa cttcattaga
      541 ccctacaacg tctgaacctt taaataaggt taaaagcatt tctagtacag aatcgactat
      601 atacatagcg gcgcttatta agaatgttga tgatggagca aatattgtag ctgtatggta
      661 tcatctggat tctgctgaat cggtggtaac agatcctctg tacattaatt atgatgcgtg
      721 ggtaaatttc agtattgatc gaattggttc gaatttccct acaggcagtt atgttgttga
      781 gctgtatgta gatgattatc tggaaaaaac attggaattt tcagtctatt aacttaactc
      841 gaaattgcat tacaaaaatc agcagcatct gtttcaggtg ctgctttttc tataatccaa
      901 aggtgttcct attccttctt ttttaaaaac ctgatacatg taatagtggc ttgaaaatcc
      961 agaatcatac gcaacttctt caattgtcat atcagaattt tttaacagtt tttttgcttt
     1021 ctcaatccta tagttgtata gatatgaatg gaaagtgcaa ttgaatcttt ttatcattat
     1081 tctgtttatc tgtcttggtg acaagaacag gctcttctcg acatccttaa gtaatatatc
     1141 atttttgtag tttgctttaa tataatttaa tagttttgta attcttaaat catcagcttt
     1201 cctttttata tatccataat catatttcat attatcatct tgtgataaat gtctgaatat
     1261 taaagtaaca atcttcataa tcataagctg tatatttata taaaacccag gacttcttgt
     1321 ctggtcttct tcaaaaatat cttcaaataa tgaaactatg tcagcatcac tatatgttcg
     1381 ttgatatatt ttagataagc tttcaattaa aaacttgcct tctttttttg tatatgacgc
     1441 atcatcacat ttttcatcta cagtaatatc gcattcaaga caatactctc tcatcggttc
     1501 tttcactgaa gatctctgcg tatgcttgac ataaggtcct gtaatgtaga aatcattgct
     1561 tttaaggtca actaaaactt cttctatttc tactgttccc tgtccagatg ggatgatgtg
     1621 aaattcaaaa tcagtatgag agtgattgga tacataccag tcagaatcag taacgacatc
     1681 aactaatgaa actattttaa cttttataca tccaagtgat atttccatat taagatcttt
     1741 tagagctgtt ttaattttca tttttaatat tgtcactttt tcttaataat atgtccattt
     1801 acataattga ggtattttca atcgcattat acaattaaaa taacaatttg tacacaaaac
     1861 aatcatttat ggaggactat tatggaaaat gtaaataatc tgcttaaaag acttagcgat
     1921 ataaagagcc agagttattg ggctgataga attatttctc aacttgagta tgcctatgaa
     1981 agcgaaaggc tttacaaatt cgggtatctt gaaaaaataa atagtgcaat acgttttgta
     2041 actcagaata ataccgagca aggcgtaata acaaaaaata ctgtcttaag ttgcgagaaa
     2101 agccttatgg aattatcttc ccatatgaag aagttcagtt tgttatgtgt atcacatgca
     2161 catattgata tgaattggat gtggagatat gatgaaacag tagctatcac cttggataca
     2221 ttcaggacaa tgctggatct aatggatgaa taccctgatt ttacctattc ccagtctcag
     2281 gcttcagtat ataaaattgt agaagaacat gaccctgcta tgcttgaaca aataaaaaag
     2341 agagtaaaag aaggacgatg ggaagttaca gcatctacat gggttgaagc tgataagaat
     2401 atgccttcag cggaaagcaa agctaagcat atactttata caaagaaata tctttcaaag
     2461 cttttagata ttccatatga ctatttcgtt ctggattatg aacccgacac tttcggacat
     2521 agcgtcaatg accctgaagt tttggctaaa gcaggaataa gatactatta ccatgcaaga
     2581 ggatttaaca aactagctgc atatagatgg caagcgccgt caggtgcttc tgtactggtt
     2641 tacaatgatc ctactcacta taatggaaaa atatcaacaa accctgtact tttagttcca
     2701 tctttcactt atgacaccaa acatgacaca atgctttatg tatatggtgt aggcgatcat
     2761 ggcggtggtc cgtcaagaag ggatattgaa agaataaagg atatggataa atggcctata
     2821 tggccaaata taagattcag tacatataaa gagtttttta aatctttaga aaaggctgaa
     2881 aacaatcttc cagttataaa agatgagctg aattttattt ttacaggatg ttattcatct
     2941 cagtccccta taaaaaaagg taacaaagca tctgaagcat tgctttatga agcagaatct
     3001 ctttctgctc ttgcatcagt aaaagagtta tctgattatg attcagaaaa aatccagaaa
     3061 gcatgggaaa tggttctttt taatcagttc catgacataa taactggttc tggcaaggta
     3121 tcaacacgtg aatatgctat gggagagtac cagaaagttt ttgctacagg taatgtactt
     3181 agaagcaatg taatgcgtga tatatctaaa aacatcaatc tttctgatta tattattgca
     3241 aaagatgacg agcttatgac tgtctcagaa ggagctggtg ttggatatgg tattgatgac
     3301 cttcaaatca gcaaagtatc aagaggcaga ggtccaacaa gagtttttac agtattcaat
     3361 ccctcaagtt tcgaaagaaa agaaaatgca gtaattgata tatggaactg ggattatgat
     3421 atttcatcaa tggaattttt tgatggtaaa aaacaaaagg tatctcatca gctaatatct
     3481 gataaagcaa acagaggttg gggcggtcag tattattaca gagttataat cgaagcttgt
     3541 gttcctgctt ttggttatac tacatatatt ttaaaatcaa atcctgaaac agcattaaca
     3601 tacaaatatc agccaaaaga ccgtacaata gattttccaa agtacacttt ggaaaacgaa
     3661 catattaaag tgtgttttga tgctgttaac gctacaatta cttcatttat agataaagcg
     3721 acttcaaaag aatatgttga tgcaaataaa ccgacaggag tattcagata tattgaggaa
     3781 aacacattaa gaaaaatgac atcatggata gtcggcagat atatgaatat tgannnnnnn
     3841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncaaatta agaaaagatg cagatgatga
     3961 caggccttca acaggatttg taatggctat taattctgaa aatgataaat ctttagttat
     4021 gttctctgac agtaaatatg gttttagaac acaagacaac agtctttcat taacacttat
     4081 tagaggatct tatgatcctg atccatatcc tgaaattgga ttgcacaaat tcaaattctc
     4141 aataggtctt tttgataaaa attcgctaaa cagaataacg aaggaaactt cctgtattat
     4201 tcatcctctt agttcaatgt cagtcaaacc ccagaaagga cacctgccga tgcaaggaag
     4261 cttgctggat ataaaagaaa caggttcatg tataataaca gcgattaaaa aatcagaata
     4321 tgtcaataat ggcatagtag ttaggatatt tgaagcagaa ggaaaaaaca gttcagttgc
     4381 gcttgatttc ggtaaaaaaa ttaagaaagc agaatatata agtattgatg aaattactgg
     4441 tcatgatagc aaaaacttga taattaccga atgttgtatt acaatattaa taaatccgta
     4501 ttgcattgaa acaatattga tagaatttga gtaattttca gattattctt gcctcattat
     4561 ccatagataa atggcgaaag tttatttgct ttcgcttttt atctatataa aaatagtaaa
     4621 aaagctattc tttaaagata aatttaaact ctgctccctg acctatttca cttactactt
     4681 ctatttctat gtcatgtctt agcgctatct gccatgctat tgccagtccc agtcctgttc
     4741 cagtcctgtt gctgtcatta tcaattttgt aaaagcgttc aaagatatac ttcatatcct
     4801 ctttttttat accgcagcca taatctttta tacttatgaa atccctgtta catacaacct
     4861 caacatttcc attttcattg gaaaatttaa ttgcattatc caaaacaacc atgaacatct
     4921 gtcttaatcg tgcgtaatca ccctgtattt ccctgctctc actcatttca aacctgacat
     4981 caatattctt cttctttgcc agatttcttg ccgtatgtac cgcatccgaa actacttcac
     5041 aaacattaac tttttcttta tttacagaaa agtcagtatt ctgcaatcta gataactcca
     5101 gaagatcctc aactaacctt tgtagcatta tgctgtcatt taaaatctgt ttatggtata
     5161 gttcaatctg ttcaggttca gtaataattt tgtcacacaa ggcctcagca gaccctctta
     5221 taaccgtaac aggagttttt aactcatgag atatgttggc catgaaatcc tgtttcatct
     5281 gtatgagtcg ttcgctttct ttactagcca tatccaattg tatgcttaac tggtctatag
     5341 ctgatgcaag ctggcctatt tcatcattct gttttactcc agtcttcgcc gaataatctt
     5401 tttcagataa agatattgca gcatttctca tcttttttaa tggattagta aaagatatag
     5461 ataagattat cccaagtatt atagctatcg ccaatgcgga taatgcgctt attgccatta
     5521 tagatatacc ctgacttata ctatcagtca tcccatcaat tgacgaatga agcaatactg
     5581 ctccttttac acttccatta attataatag gaacacctat agttaaggta ggttcttcta
     5641 taattccgct aaactcttca ctaaaagtcg tctttccgct aaaaacatca ttaacaagca
     5701 cttcagcatt tgaaggcagg tcagaatatg taacctgatt aactcttcct cttcctgata
     5761 ctaaaaaatt gagattttca tcaacaatcc atacatcatt tgttgtaaca tcctccagta
     5821 ctttggtata agcggaaata cctccctgtc tggaccatcc tccgctttca tccaaaaatt
     5881 cagagatgct gtcagctatt gaagaagctt ccttttgcat atctgattta tagaaagata
     5941 ttatatgact tctaaataac ataacgaata aaaagcttat tactattaag aataatacta
     6001 atgtaataga aaagtatgca gaaagcttgt aagccatttt attcttcatt tttaatttcc
     6061 tcaaacttat accctactgc ccatatagtt ttgacatccc aactttcatg cttatactca
     6121 ttaagttttg ctctgagcct tttaacatgg gtatccacag ttcttgtgtc tccgtaataa
     6181 tcatatcccc aaagcgaatt aagcaggctg tctcttgtaa atacctttcc agctgacgag
     6241 gcaagagtcc atagaagctc cagctctttt tttgtcagaa gtatattatt gttatcaata
     6301 cttaccgtgt aatcatcaat attgattact agattactaa tattaagtat ggatttattg
     6361 tcttttattg agtcttttga taatctacgt aatactgctc ttgatcttgc cattacttcc
     6421 tggggggaga aaggttttac gatgtagtca tctgcgccta tatccagacc cattattttt
     6481 tcaaagtcct ctccccttgc agtaatcata ataattggta catcagaagt ttttcgaatc
     6541 tctctaacca cttcaaatcc atccacttta ggcatcatta catctaaaag caataaatca
     6601 taattactat ttttgaattt ttccagcact tgagtcccgt cataaacaac tgtgacagaa
     6661 tacccttctt tcttagcgta ttcctctaaa attgaagtga tttgtttgtt atcatcagca
     6721 attagtattt gtgtcatacc tagcctccat ttatatattt aattatacca tttaatgtat
     6781 attcatttgt gacaaagata tggcacaaaa aatttagtat tcacaaaaga tactaacatt
     6841 tgacacactt ttgacacaca cagcatatat catatagttg caggtaagat gaactgcaga
     6901 agtaaaaaag aaaaaggagg tcaataaaat gactaagata aaaaaaatat tcataatcgg
     6961 gctagtaatt ctctcattaa gcgcaatttc ggtaacatca tttgcagcta cagagtataa
     7021 aaacccagca gaggtttact ctgcattaac agaaactcct gtggaagatg tgctcgcttt
     7081 aagacagaca tctggaatga cttttggaca acttgcttta gaagcaggaa aatcagaaga
     7141 tttcaaagct ttggtttttg aaatgaagaa acagattctg gctgacaaag tagaagctgg
     7201 tgtaattaca caagaaaagg ctgatgaagt attagctata cttgaagctc atatagctga
     7261 ttgtgatgga acaggctttg aaggcgcaaa agcaggaatc ggaattggat ttggaactgg
     7321 aacaatgcag aaacaaggca acagcctttc aagaggtggc cagggtggaa tgcaaagagg
     7381 ttcaggacaa agacagaatc ttagaaacgg atcatgtctt ggcaactaac aactaaaccg
     7441 gataattaat ccaaacaaag aatgcgcatc catcaatcat gggtgcgttt cttttattcc
     7501 tatataagca aaatatttta tgacaaattt tagaattgtt ctattgacat atattctaaa
     7561 attatatata tttctattta atgcgtgata taattgacgt atattaagat acctttactg
     7621 ttttactgct gtatacagca gatggtttta gacctttaga tgaattactt aatgaagccg
     7681 tacttcattc gggttgaagt tttctacata tattggtagc ggttaacaca gaaaggagta
     7741 aattggataa tacaacttta tatagaaaaa agaagatacc gattaaacga atagtcatat
     7801 atatatatgt gattattgtt gtagtagtat tatccataat tgcatatagc ttattcagtg
     7861 gattatctaa aacagcaaac aaggaggcac aaagagcctc atttgaactg aatgccagca
     7921 tttataatga aatttataac tacattgact tttcagaaca cataaatcat gtatatggtg
     7981 atcttgtttc caataatctt ataaactttt ccgataaaga tactagagag actcttatta
     8041 tgagttttat gaacaatcac agcttaaata tatctgtatt cgcatatata aatgagaaca
     8101 atcagtttta cggaggaata agaagagatt ctgatacact agaaatatat accttagacc
     8161 agacaaccaa ctggcagttc tctgttcaaa atgtgaatga tgaaaataaa gatataatta
     8221 ttggaagcga tctcattgat ttcaaaaccg agaactggta cgaattagca aaacaaacaa
     8281 acgaaaacat aatactgcca gtatattaca acgaacatat ttcaatgcat actatacgct
     8341 cagtatttcc aataaatgat gacaataatg tatttcaagg agtcatatat cttgaaatca
     8401 atttagataa catgcaacag ataatcgagt cacttattga agacgacaat tatagcgctt
     8461 acataattga caaaaacaca aacacagtcg ttatctccac taatgagtta tatccgttag
     8521 gatataatat aaacgaaact gatgatttct tggaatatca gcaatatttt gatacctcag
     8581 ataaatcatt ttcaaaagga ggattcattc agggcagtta tcatacatta gaagaataca
     8641 aaaacgatga tataaactgg gtcataatga catcagtacc caatagactc tttttggaag
     8701 gttttagtga taatgtaagt atattttccc ttcttacagt agtggtcgtt gcaatatctt
     8761 ttcttctgct tcatataata tccgataaac tagtcaggcc acttaacaat cttgttaagg
     8821 ctgccgagga aatctctgca ggaaaacttg atatagacat accaattaca cgtaatgatg
     8881 agattggaat actatctatt gcatttaata aaatggcttc ccgaataagt agtctttttg
     8941 aaaatctaga aaataaggtt gaggaaagaa caaacagttt aaatgaagct aaagaaaaat
     9001 tacaaattat tcttgattct actgctgaag caatttatgg cgtggatctt gatggaatat
     9061 gtactttctg caataaaagc tgcttaaaaa ttttgggata taccaacgaa gaccagcttt
     9121 taggaaaaag aattcacaat atgcttttta atcctgatga tgaaaaacac agcaactgca
     9181 gagttgtaga cgaaatgaaa aagggattgg ttattcacga ggaagatgaa gaatacttaa
     9241 aagctgatgg tactaaaatt tttgttgaat atcattcata tccgcaatat aaagatagtg
     9301 aagtaatcgg agcagttgtt acctttcttg atgtaactga aaaaagaaag agtacagaaa
     9361 aaatacgtta tcttagttat catgatgttc ttactggttt accgaacaga agaagttatg
     9421 aggaatttat caaaaaaact gataaacaca aaaatattcc atattctgtt atttttgcag
     9481 atataaacgg gcttaagatg actaatgaca tattcggaca tagcattggt gatgatttaa
     9541 tcattaaggc ttctgaaata ataaaaaagt gtgctgggaa aaacaacttt gttgcaagaa
     9601 ttggtgggga tgaatttatt gttctattac caaagacaga ttatgaaaaa gcttctgaaa
     9661 taacagctaa aatcaaagat atgatcagcg aaacaaaaat cgttgctata atgtgcagta
     9721 tttctttagg ttgtgatacc aagtatgatg acaagacaaa tcttgaacag gtggtaaatt
     9781 cagctgaaaa taagatgtac aggcaaaaag taatgaatag aaaaacagta aattcaaata
     9841 caataaatac tatagtcagt actcttcatg aaagaagccc aagagagctc acacactcaa
     9901 acaatgtttc atcacttgca gcaaaaatcg caaaagcaat gaatctttca gaaactgaag
     9961 ttagaaaaat tgagatggct ggatatcttc atgatatcgg taaaatagtg ttaaataatg
    10021 acatcttaaa aaaagacata tattctgatg aagaacgaga agaatttcat ttacatcctg
    10081 tacatggtta cagaatactt aatttattcg atgatactct cgacatagcc gaaggcgtat
    10141 attcccatca tgagaaatgg aatggcaagg gatatccaaa aggacttaag ggttctgaaa
    10201 tcccattaat agcaagaatt attgctgttg ctgaagcata tgatgcatta attaacagta
    10261 aaacggtaaa acctatttcc aaaaaacagg ctttggataa cataaaatct atggcaggca
    10321 ttatgttaga tcctaatata gtcaatcttt ttgttgatat aatgtctgat aacgtataat
    10381 atttatatat gttactaggg gcaaattttt aaaaatgaat aaaaataatt ctatgtcaag
    10441 atttttaaca aataacaata ggtttaagaa agataatccg aactacagta gagtttatct
    10501 tcttaacgca gcattgattg tattgtcgat agtttgttta ttttttgcag ttttaaactt
    10561 tttggaaaat gatgtgccct ttgttgtgct acttgatttg attataatga tatgtgcaat
    10621 tctaacaata gcaaactttc ataaaactga taatatcaaa attacttcat tccttacgac
    10681 cattctgtta acctttctaa ttgcaatata tttctacttt gttgaaaata ggaattttgg
    10741 attattctgg ttttcaatct atccaccctt tacatatctt atatttggca gaaaaaaggg
    10801 aaggatatat acattgacat tcggctcaat tattttgact tatgtaattt tatgttacaa
    10861 atcatgggaa cctgctgttt ttgattatca atctttaata aatattattg ctgcagctat
    10921 ttgtttatct ctattaatag ttttttatga aaacagcaga aaagctgctg aagatgcgat
    10981 aattatgaag aataaagaac tcgaaattat gtcaatcact gatcgtttaa ctgggttata
    11041 caaccgacac aaattagata tcgctcttaa tgatttttta tcaaaaagcg acaattgcaa
    11101 taacatattc tcaataataa ttgcagatat agactatttc aaatccttca atgatactca
    11161 cggacacctt tatggcgaca aagtactagt tgatttggca aacattataa aagcaacagc
    11221 tggcgataaa aatatagtcg gacgatgggg aggagaggaa tttctaatca tttgcccagg
    11281 tcttaataat gaaggtgtaa aagagctagc acttaagatg cttgataata tttctaacta
    11341 caaaactaaa gacaataaaa aggtatcaat gagtttcggt attgcatcgt gtcaagcaga
    11401 tgaaactata gatacattat tgcaaagagc tgatgcttct ttgtactgct caaaagacaa
    11461 gggaagaaat atggttgatg gaaaacctat atgtgatagt tgatatttaa ttaatgtttt
    11521 ataagaaaga ataaagtatt tttatagata aataataagg tgctgatatg tataaaattc
    11581 ttatagtgga agatgatctg gttatagctt catcattaaa aaaacatata gaaacatggg
    11641 gttacgattc aaaatatata accgatttca agaatgtcct tgatgagttt tcaacttaca
    11701 aacctcatct tgttttactt gatgtatctc ttccctattt taatggatat cattggtgtg
    11761 aaatgataag aaaaatttca aaaacgccta taatattcat atcctctttt tctgagaata
    11821 tgaatattat tatggctata aatatgggtg cagatgattt tatatcaaaa ccttttgatc
    11881 ttgatgtact cacagcaaaa atcaatgcac tattaagaag gacttatgat tttaaaccta
    11941 acgatgaatg tttaatagca aatggactta tccttaatac taataacctg tcaatggaat
    12001 ataaagatac tgtttatgaa ctgacaaaga atgaattcag aattcttcag gttttgatga
    12061 gttctaaagg gaaaactgta aatcgtgata cattgatgtc taaactgtgg gaaacagaca
    12121 gttatataga tgataatact ctgactgtta atataaacag gttaagaaaa aagcttgaag
    12181 cagtaggtct taataatttc ataataacaa aaaaaggaat cgggtatctt gtaccatgaa
    12241 actttttttt gaatatctta aatatcacat aaaaagtata attctgtttt cttcattttc
    12301 atgtatattt cttatcgtat tcatactgta ctccgttgaa ccgcaaccgg ctttgtatgc
    12361 ttcttttata tgctttgttt ttggccttat gttagctatt ctgagcttta tcagatttct
    12421 ttctaaacat aaatcattat cagaaatgaa gaataaaatt actataacta aagatgacct
    12481 cccagtacca aaaaacctta tagaacagga ttatgaagat cttattactg ttttgcacga
    12541 tgaaaaaacc agtatagaat caaaaaaagc aaatacgatt gctgaactta atgactatta
    12601 tacaatgtgg gtacatcaga taaaaacacc tatatctgca ataaagcttc tttttcagac
    12661 taatgatatt catgatgtag aaatagagca gcagcttttt aagatagaac agtattctga
    12721 aatggctttg cagtatatcc gtactgaatc agatgttgct gattttctaa tccgtgaata
    12781 cccattagat gcaataataa gacaagctgt aagaaaatac tcaaaatcct ttattaccaa
    12841 aaaaattgct ctagattata ctccgacaca gaaaacagta ttaacagatg aaaaatggct
    12901 tttattcgtt attgaacaga tactttcaaa ttctttgaaa tatacagact ctggaaaaat
    12961 atcaatatac acaaaagaag atgataaata tacattagtt attgaagata ccggaatagg
    13021 tattgaatct aatgaccttc caatgatatg cgaaaaagga tatacaggga taaatggaag
    13081 atatgataag aaatcaacag gtatcggact atatctaagc aaaaaaatac ttagtaaact
    13141 ttctcatgag ttagcaatag aatctcaacc tggttctgga acaaaagttt atatcagttt
    13201 ttcttcagag gaaagaatat ctgactaact tacaaaaatg taagattaat acgtattttg
    13261 taagtatatt ttatggataa cctcttatct tttatgtgat aatatctgca aacaggaggt
    13321 tttatatgct tttagatgtt aaaaatctta aaaaaatcta tactacaaga tttggcggac
    13381 acaatgtgca ggccttgact aatgtcactt tttcagttga ggaaggcgaa tttattgcaa
    13441 taatgggaga gtctggttcc ggaaaaacca cgcttctcaa tataattgct gctcttgaca
    13501 aacctacttc aggtgaagtt ttcctcaagg acaaatcatt aataggtatt tctgaaaaag
    13561 aaatttcagc tttcagaaga gataatcttg gttttgtttt tcaggacttc aatcttcttg
    13621 atactttctc tgtaaaagac aatattcttc tcccccttgt attatcagga catacatatg
    13681 aagaaatgaa gaacagtctt aatactgtgg ttgagaaact aggaatatca gatcttcttg
    13741 agaaatttcc atatgaaata tcaggaggac agaaacaaag aacagccatt gcaagagcta
    13801 taattacaaa tcctaaactt ttacttgcag acgaaccaac aggagctctt gattcaaaag
    13861 caacagaatc gcttcttaaa ttgttcaaga aaataaatga agatggacag acagtattca
    13921 tggtcactca ttctgtaaaa gctgcaagtc acgcatcaag aattttattc ttaagagatg
    13981 gagcagtatt tcatcagata tacagaggtt cctcatcaaa tgaagaaatg tatcaaaaaa
    14041 tatccgatac attgacagtt atagcgacag gtggtggctt caatgcgtaa ctgcatgtat
    14101 tttaaacttg ctgcaataaa tataaagaaa aatgcaaaga tgtatatccc atatattatt
    14161 acctctatca ttacaataat gatgttctat attatgattt tcttaacaca caatcctgga
    14221 ttggttggaa tgaaaggtga acattcatta agaactgtat tagtgatggg aatatatgtt
    14281 ataggtattt tttctgttat atttctattc tatactaaca gttttctact caaaagaaga
    14341 aaaaatgaaa taggcttata taacattctg ggcatggaga aaaaacatat agcccgaatg
    14401 ttaactatag aaaatatata tacttatctt ataaatatga ttttgggtct attcttcgga
    14461 attctgtttt ccagacttat gctgctttta ctctttaaaa ttttgaaggc aggaacaggc
    14521 ttcgttttta atatttccat gcagggatta tatatgagtt tcattttttt cactgcaata
    14581 tcagttttaa ttcttctaaa caacatccgc cagatacatt tgtcaaaacc ggttgaactt
    14641 ctaaagggag gaaatgtagg agaaaaggaa ccaaaaacaa agtggctttt agcagttata
    14701 gggattatta caatgggtac aggatattat ctggctataa ccgttgataa tgcaatctca
    14761 gcgcttaata atttctttat agctgtaata ttggttatca tcgggacata ttgcatattc
    14821 actgcattca gcatagcttt tctgaaaata cttagaaaga ataagaaata ttattacaag
    14881 gccaaacatt tcacttcagt atcaggaatg atttacagaa tgaagcagaa tgctgttggc
    14941 cttgcaaata tatgcatttt atccactatg gtactggtaa tgatatcttc aacggtatgt
    15001 ctgtatatcg gtcaggatga tgttctgaaa aacagatttc cgacaaatat tgaaatccaa
    15061 accatatttg caaacgaaga aaaagctaaa gcagtcagta agaccgttaa tcagaagata
    15121 ctggaaagcg gatatgaaat tgatgcatat aatgaatttc gattcaaaac atactctctt
    15181 gaattcgata ataaaaatca tgtattccac cacagtaacg atatttctct gagtctggat
    15241 aaaatgtata ttacatatat gtttccaatt gaatcatata acataaatac cggttctgat
    15301 attattctta atgataatga agtagtcata tacagtcctg atgatattga tttatcagac
    15361 aaatttaata ttaatgataa aacattctat gtaaaaaata ttgttgaaaa ccctaatttt
    15421 tcaatacaga acaatggatc atatatgaaa cttatttaca ttttcgtaag caatgaagaa
    15481 gttataaaag acattcataa agattttgtt gaatattttg gtgagtattg tgatttatcg
    15541 tatctaatag ggtttgatgt taaagaacct gaggaaaaac agattgaact tatgtacagc
    15601 atacgtgatg aacttagaaa tacagatgaa tataagaacc tgtcttttat catgtacgca
    15661 actgcagagg aaaagcttga tttttacgca atttatggtg gcttattctt tttaggatta
    15721 tttcttggcg cattatttat gatggcaaca gttctgataa tttattataa gcagatttct
    15781 gaaggatatg aagacaaaca tcgtttccat atcatgcaac aggttggaat gagcaagcat
    15841 gaaataagaa aatccatacg ctctcaggtt ctaacagtct ttttcttgcc tcttgttact
    15901 gcaattgtac atatattatt cgcttttaaa atgattacaa aaatgcttga ggttctaaat
    15961 cttacaaatg tcagcctatt tattatatgc acaatagcaa ctgtaataat atttgcaatc
    16021 ttctatgtta ttgtttattc acttactgca agaacatatt acaaaatagt agaataacta
    16081 cccaaccttt acaacatatc tgttaagata acagggatat ttcgtaaggt cctccctata
    16141 aggagctaaa accacgagat tccctgttag cttatcttca tatactccat gatttgaata
    16201 atcaacgaca ttggaagtct tttccatagt ttcatgattt gatctctttg tatctataac
    16261 cataactgta tctttaataa ccttacaaga tttcttatct acttcggcaa tacacaaagg
    16321 gtatcttggc agattacctt caggattttt ttcatttata acacctatat agtaaagctt
    16381 attattgctg tgattaagca atttcggaat acatgatgat gaatacataa tactaccatc
    16441 attaaatgta agcattttcg gttcagacca tgtataaccg ttatcatttg aaactgaata
    16501 aaacttggtt ccaataattt tatccgattg tctgtaatta ctgttgcgca ttatcattat
    16561 tactttattg ttatctaaca aaccgaatgt aggttcatac aatcctctta ctgacttatc
    16621 cagtcctaca gtaacatact gatttatgtc ccactcatat tctaatttat ctttattcca
    16681 tgtacctttt aaagcaccag ctttcataaa acccatacct gtcggattta gtaatgttcc
    16741 atcatcattc aaaacctgta tctgaacact tagaagcaat gtgccatctt gagatcttac
    16801 tactaaagga gataccatgg ctgccatgtt cttttcaaac tcaacttctt ttataaaatg
    16861 agttttatca taactcatac ccttttgaat tatatatttc ttatctgtcc atgtatgacc
    16921 attatcgaat gaaatacggt aataaagggt tctcatacgc cacaaggaag caagtttgtt
    16981 ttcttcccag tatgtatcat tagcgaaata caccattaca tttgcaactt catctgtata
    17041 tagtgctcca taaccgcttt ttacccagct gtttttaaag tcataaacat atgatttctc
    17101 tatcatatgc tgttcttgcc atgtcagtcc gttgtcaaga gatcttagta taaggcttcc
    17161 ggtgttactg tctgaaccat taaaaagatc aaaattcatt atcatttcat ctgaattatt
    17221 gcagtatgcg actgtcggcc ctaatatgaa ataatcctta taactcaaat acttatcctg
    17281 tttttctaat aattcaaatg gcatgttaac ctccgcatct tttttatgat tatatcatct
    17341 taagcaccaa tattcatgat aatttaatag ctttaaaaat atagtataat aaagttgggg
    17401 tgaatatatg agagcatttg aaagagttaa ttgtggaata aagagtatgg atagtctttt
    17461 agataatatt aggcttggag acaatgtagt tattcaggtc agtcatctca aagactacag
    17521 caaaattgca aatctttttg ctatgcagtc gataaaagat ggtaaatcag taaattatat
    17581 tcgtttcgga tcacatgaag ctgtattaga agaacaaaaa ggaatcaaga tttatactcc
    17641 taacccaaac tcaggttttg agagctttac aatggaagtc agaagaatta tagagagtaa
    17701 cgggtatgat gctttttaca tctttgactg cttgtcagat ttacaggcaa tttggtcaac
    17761 tgatttgatg atgggaaatt tcttctgtgt cacctgccct tatctttttc aattaaatac
    17821 agtagcatac ttccctattc tcagaggtca ccatgattac tctacaatag ccagaatcca
    17881 aaataccaca cagctgctac tagatatcta ttcgcagaac aatgagctat atctgcaccc
    17941 catcaaagta tggaaccggt attctccaga catgttttta cctcataaac tggatgattc
    18001 tgaagacttc ataccactgg tgaatagtat tgatcttaat aattactata gtttaattca
    18061 aaaagaacag tctaaaacac ccttactaaa cattgacagt tatgaaagat tctttataga
    18121 aatcaagcag aaacataaac aaggcaccat aagtaatgat gacattgaca gagtcattaa
    18181 aagcatgatg actcatgatg agtttattga aactttaatt aaaaaccatt tttcctgtga
    18241 tgatcttttc tatattaaag aaagaatgat tggaactggt tctataggag gaaaggcctg
    18301 tggcatgctc cttgctagaa aaataataca caatgacaat ataaattctg aagacttttt
    18361 agaacctcat gattcgttct atataggaac tgatgtcttt tattcatttc tggttgaaaa
    18421 caatttatgg actttacata taatgcagca aaaggatgaa tactatttta aaaaagctga
    18481 tgagttaaaa gaagcaatac ttaatggcag atttcctgag aatatacgtt cacaatttcg
    18541 acgtatgctt gagtacttcg gacaaattcc cattattgta aggtccagca gttttttgga
    18601 ggatggattc aaaagcgcat ttgccggcaa atatgattct gttttctgtg ctagcaaggt
    18661 tgatccgata aaacggctgg aaagatttga agatgccata agaagagtgt atgcaagtat
    18721 gatgagcact tcagtattag agtacagaaa aaaaagacaa atggataaaa ccgacgaaca
    18781 aatggcaata ttagttcaaa gagtcagcgg aacaaaattt gacaattact atatgccctg
    18841 tgttgcagga ataggttttt catacagttt gtacagatgg agcgctgagt tgaatccaaa
    18901 tgccggaatg ttaagactag tagcaggact tggaaccaag gcagtagata ggacaggata
    18961 tgattatcca aggcttgtta acctggatgt acctgaaaaa acaacattag ttactgacga
    19021 agaaaaacac aggtattcac aacgtttcat tgatgtaatt gatttggagg agaacaaatt
    19081 tacagaaatc gattttgaaa agttgctttt atcacttcca aaatggtata cagacctcat
    19141 ctttgaacac gacagaaatg ctgaagccaa tttaagagat tccagaataa gaagaaatgt
    19201 actgtatatt tcctgtcata atgtggttaa aaataaaaaa ctcatggaga tcatgtcata
    19261 tattttagca aagcttcagc aggtttattc tactcctgta gatattgaat ttactgtaaa
    19321 cttttctcca gagggaaatt ttcagataaa tcttgttcag tgcagaccac tgtatatatg
    19381 gaaaaaccag aaaagcatta ctattcctga gcataaattt tcagatatac tatttaaatc
    19441 acataacaca tttatgggca ataaacccca tatatctgta gatactgtaa tttatgtaga
    19501 agcacgcaaa tatcacgaac tatcatataa gaagaaaagt tcagttgcat ctgcagttgg
    19561 aaaaattaat aagtattata aaaacagtaa taaaactgta cttctactta ctcctggaag
    19621 aataggtact tcatctaatg aattcggcct tacggtcagt tttgcagaaa tttcaaattt
    19681 taaaatcata tgtgaatata ctgataagaa agcaggtttc tttcctgagt tctcttttgg
    19741 cagtcatatg tttcaggatc ttgtggaaaa tgaatttttc tatatagctc tagatgataa
    19801 tgctgataat atactaaaca ctgatagttt tattaaatta aatgatatta catctaggtt
    19861 ttctgaagac ataaatgatt atgaatgtat tcggattttc gaaaatgatt taaaaacaaa
    19921 aattgatttg tatgcagact tcgataaacg gatatgtgtg ttgttaagaa gatgatgaat
    19981 ttttgaaaca ccagacaatt ttatacattc gattcgctgt ttcttcatct atgtttattg
    20041 aatttaaata tttctcacac tcatttagaa tttttatata ttctttactt tggttattat
    20101 aataaatgta atcatcttca aaatcattat taagcatatc aatcctaaat ttaattttat
    20161 ctaattgata gcctaatcct tttagaactt taattattct tagtttttcc aaatcttcat
    20221 cagtatatat cctatatccg tttagttttc gcttaatttg aattaaattt ttcttctccc
    20281 agttccttat agaatctggt gtagtgttga tatattcaga tgcttttttt cttgtgaagt
    20341 atagttgatt ttcttgacca ctcttacttg taattacagc atcttttgct ttcttaatct
    20401 cttcaacatt aatcttctct tgtcttatat atgagataag ttcattaata tataatagtg
    20461 aagtgtccag atcactatct gcgcatgctt taactgactg tattgctttt tttctaatat
    20521 acttatgctg cttgttcata ctaagagcat gtcttgccag tttgtattct aatacatgct
    20581 tatcattaaa taccctgtac ccgttaacaa gtctctttgc aggtgaaaca aggcctaaat
    20641 cctcatataa tctaactgta ttcgaatgta tacctgtaat tttcgctatt tgtgaagttc
    20701 tgtagtttgc catttgtcac ctcaataagg ctggtattct aatgtatggt taaatcatat
    20761 cacagacact gttgtaaagt aaatatttct ttacagaaat aaatagtatt attgttatga
    20821 atagtttacc tatacaagta atatgcaaat ttctgcttct aatagacctg aagtgaagaa
    20881 taactaatca gaaatctacc tgtaattaca acttacaggt tttctattag taaaattcat
    20941 aattcttatc aataaattta ttgctttata tatcaattta tgaaagtctg tttttgaaat
    21001 cctcataccc aaatttctta accagcttaa aatctgtact attctgtgcc agatatatcg
    21061 aaggtaatgg cattccatta aatgtgttat tcttaaccat tgtgtaaata gccatatcac
    21121 aaaagactac tatatcccct gttttaagtt tatagtcaaa agaataatct gagattacat
    21181 cacctgcaag gcatgtaggt cctgcaagtc tgaaagtata tttcaactca ttgggcattt
    21241 tagcatttaa aacattagga cgatatggca tctccagtac atcaggcata tgacatgctg
    21301 ctgatgtatc cattattgca atatccattt cattgtgaat tatatcaatt actttcgata
    21361 caagaacacc tgcatctaaa gcaatagctt ccccaggttc aaggtatact tccacattat
    21421 atttctcttt aaatgctttt atgcattcta ttaaagctaa gacatcataa tcttttctgg
    21481 ttatgtgatg ccctcccccg aaattaaccc atttcatctt tttaatataa ggaccgaatt
    21541 tttcttcaac agcattaatt gtttctatta aggcatcaga gttctgttcg caaagagtat
    21601 gaaaatgaaa accggttatt ccatcaagaa gatcttcttt gaaatttttc tttgtcactc
    21661 ctaaccttga aaaagctgag cagggatcat atattgcatg attctgtgtc gagtgttctg
    21721 gatttatcct cataccaaat tgcctgttac tttttaaagc cttgtctttg aacttttccc
    21781 actgggaaaa agagttgaag attatatgat cacaaatttc taaaatttca tcaaattcct
    21841 cttctttgta ggcaggtgaa aaaacatgat tctgcttctt catatattca tatcccagtt
    21901 ttgcttcata taagccagac gaagctgtac cagaaatata ttctgctatc aaaggataaa
    21961 cttcaaacat tgaaaaagct ttttgagcaa gtagtatctt acatcctgaa gttttctcaa
    22021 catatcttaa aatttctaag ttactgatca gttttgattt atcaactaca taagcaggag
    22081 tatttatatt gctaaagtca tattccatct attctaccaa tttaggattg aaatccaatt
    22141 gccaaggcaa tccccattta tttaatgctt ccatgaaggg gtcaggatca aactgctcca
    22201 tattgaatac gccaggtttt ctccattgac cgttcagtac cagcatagtt cctatcattg
    22261 caggaactcc tgtggtgtat gatattgcct gactttcaac ctctttgtat gcttcttcat
    22321 gatcacacac gttgtaaata taaagtattt tttctttatt atcttttata cctctaaata
    22381 tacaccctat attggtcttg ccttttgttc tgggaccaag cgaagcaggg tcaggcaaga
    22441 gtgcttttaa aaactgtaaa ggaactatct tcatcccatt gtaatctata ggttcaatag
    22501 aggtcatgcc gacattctcc aaacatttaa ggtgagtaag atagcttagc ccaaaagtca
    22561 taaaaaagcg gattctcttt actcctttaa tatatttagc aaggctttca atttcttcat
    22621 gatgtaaaag atacatatcc ttaggaccta tctgaggaaa atcatatacc ctttttatct
    22681 gcataggttc tgtttctatc atatcaccat tttcaaagta agaacccttt gctgtaactt
    22741 cccttatatt aatctccgga ttgaagttgg tagcaaacgg atatccatga tcacctgcat
    22801 tcgcatcaag tatatcgata taattaattt catcaaaata atgtttctga gcataagctg
    22861 aaaaaactga tgttactcct gggtcaaatc cgctccctaa gagtgccgtt attccagctt
    22921 ctgtgaatct ttcgttatat tcccactgcc atttgtactc aaactttgct gtatcaaccg
    22981 gttcataatt cgctgtatca acataatttg tttttgttgc aagacatgcg tccattatcg
    23041 tcaaatcctg atatggaaga gcgagattta aaactgtatc aggtttaaat gaatttatta
    23101 aagcaactaa ttctttgaca ttatcagcat caacttttgc agtatggata atagttttct
    23161 cattttccag tttctttttt aaatcatcac atttactttt agttctgctt gcaatcataa
    23221 tttccggaaa tacttcgtaa ttctgacaac atttccttat tgcaacatta gcaactccgc
    23281 cacatccgat tattaatact ttactcatta caattacccc ctatcatgat taaaatctct
    23341 cttgttatct tgcatgctgc tgctgtagat gctcctgaat ggtcgtagtg tggtgataat
    23401 tcaactatat cacatccaac aacttctaac cccttcagtt ccaaaactgc atttaaaagc
    23461 tccttaaagc ttacccctgc cggttcaggt gttcctgtcc cgctaaaaaa tgcaggatcc
    23521 agcacatcaa gatccaaact cagataaaca tgttttcctt taagtttttc ttttacatcc
    23581 ttaacagaat ccaaatcaaa cttatacata ttagtatgtt tatcagcaaa ttcaaactca
    23641 gatttttcac cgcttctaat tccgaactga taaatcctat catctcctgt tatttcccat
    23701 atccttctca ttactgtcgc atgtgaaagt ttctgtccta gataatcatc tctcaaatca
    23761 gcgtgtgcat caaagtgaac aattacaagg tcttgatatt tttcaactaa tggctttata
    23821 gccgcataac ttacaaggtg ctctccacct aaaagtactg gtatctttga atcaccaact
    23881 acacttcttg acatttcttc aatcatgtac agtgcttttt ttgtgtcacc aaaaggaagt
    23941 tcaatatcaa atgcatcatg tattttgaag tcagataaac ttaaatcctg atatacggaa
    24001 taatcctcta ttccaaatga ttcgctcctg atagcactgc ttccaaatct tgttcctggt
    24061 ctgtatgatg tagtattatc aaaaggagca ccatagatta ctatttttgc ttcctcataa
    24121 cttgaagtac aacccataaa gccgtttgca ttattcgaca ttttccaaca tctcctctac
    24181 gtaatttggc agataaaaag aacctttatg caggtttgta ttatagtagc gtgttttaat
    24241 gccaagagct tcccattttt gagggttcat atcatgtacc ggatgaagct ttttagatgc
    24301 aaatccgaaa agccaatgtc ctgaaggata tgtagggata aaagcctgat ataccttgct
    24361 taatgggaaa gaatcaaata tccttttatg agctcttttc attgccgcag catcttcttc
    24421 atagaagggg ctttcatgtt gattaaccat aatcccatcc tctttcaatg ctttaaagca
    24481 gttaccgtaa aattcttttg taaaaagtcc ttctccaggt ccaaaaggat ccgttgaatc
    24541 aacaataatc aaatcatatt cttctatatg tcttctaatg aatttcattc cgtcctggta
    24601 atatatattt actcttttgt cttcaaatcc acatgcagta ttaggcaaga attctttgca
    24661 tactgcaact acttcttcat caatttccga aacatctatc ctcttaatag tgggatattt
    24721 tatcagttct cttaatactc ccccatctcc tgcacctata accagaacat ccgttgtatc
    24781 aggttttaca gccatcggga catgtgtaat catttcatga tatatgaact catctttttc
    24841 agtaagcatc atgtatccgt caagagcaag aaattttccg aattcaagtg attcaaatac
    24901 atcaattctc tgaaaacggc tttctttgct gaaaagctgc ttatccactt ttatggacaa
    24961 atatacgttt tcagtatgtt tttcgctata ccaaaattcc atttaacggc ctcctatcaa
    25021 gacattaaga ttcttaattg tcatatcctc gggccctgtt atcaatgagc ccttttcctt
    25081 tgcatagact atatagtcta ttatctgttc tgttatcctc tctccaggag ctaaaatagg
    25141 tatccccgga ggatatgcca ttacaaattc cccgcaaata tgtccagctg tgctttttat
    25201 atccatactt actttctttg cataaaatgc ttcctgtgga gacatctcaa caactggatt
    25261 tatatattca tgcttcaaag ctgaagtacg aggtttcttg tatcttcttc ttatttctga
    25321 aagagatcct atcagctgtt cgatattctt gtcactatct cctactgaga tgtatgccaa
    25381 gatattttcc aaatcaccaa attcaatctg tatatcatat tcgtctctta aaagatcaaa
    25441 tacttcaata cctgctaacc ctactcctga tgtatttatt gataactttg ttacatcaaa
    25501 atcaaataca gtatcattgt ttaccagttc ctttgaatat gcataatagt cgcctatatc
    25561 attaatttcc tctcttgcat attgagaaag ctcgattaca gtatcgaata tttcttttcc
    25621 atgcagaaaa agtcttcttc ttgatatgtc cagactggac atcagtaaat atgaccctga
    25681 agtagtctgt gtaaggttaa taacttgtcg catatagtca acatttatat tatcaccaca
    25741 tagaagaaga gaactctgag ttaaagaccc cccagactta tgcatactta cagatgccat
    25801 atcagcacct gctgccattg ctgatatagg aagattgtca ttaaaataga aatgggttcc
    25861 gtgtgcttca tctaccataa caagcatatt atgcttatga gctagttgtg ttatctgctt
    25921 gaggtttgag catattccat aatatgttgg attatttaca aaaactgcct tagcatcagg
    25981 attctgctca attgccttta ccacatcatt gtatgacata cctaatgaaa taccaagctc
    26041 actgtttatc tgagggttaa tgtacaccgg tatagctcca cagagaacaa atgcattaat
    26101 aacgcttctg tggacgtttc tgggcataat aatcttgtct cctcttttta gagaatacaa
    26161 aaccatcgac tgtactgcag aagtggttcc atttaccatg aagtatgcat actttgcacc
    26221 gaaagcctga gcagctaaat cttctgcttc tttaatcact ccagtaggat ggcatatatt
    26281 atcaagatgc ttcatagaat ttatatctac agaaagacat ttctgaccta aaaagtcagt
    26341 aagatcttta ttgcctttac cccttttgtg tccgggaaca tcaaaaggaa caatatgtaa
    26401 tgtcttaagc tcttcaagcg cctcgtatat aggggcgcga ttctggtcta gtaaaatcat
    26461 ctttgccacc ttaataaacg ttaatgccgc tgaaaatttc tatcatttca cggcgcaggc
    26521 tgtttgttat tgacagcctt gtttttggtg ctatttcata aacatccgta ttaaacagat
    26581 aattctgaag atccacctct tttatcatca tctttgaatg aaaaatatta gcctgataaa
    26641 cattgatatc aactgcatca tatctttcta aggtcttagc atctatatag tcttgtatgg
    26701 aagtaatatc atgatccatg aaaagttttc tacctgtaat gtcccttgtg aatcctctga
    26761 ctttataatc cattgtaatt atatctgaat caaaattccc aatcaaaaaa tccagtgtag
    26821 acaaaggtgt gatttcaccg caagttgcaa catctatgtc aaccctaaag gtagctattg
    26881 atgtatcagg atggtattca ggataagtat ggactgtaac atggctctta tctagatgtg
    26941 ctactatctc cttattcttg tgaggaatca ttgcttcttc tgcaattaag aaagtagcgc
    27001 tggctccttg aggctcataa tcctgttttg caatatttaa aactgtggct ccaatcatct
    27061 gagtaactgt tgtaagtata ttcgtaagcc tttccgagtt atattgctcg ttgatatatg
    27121 ctatgtaatc tctttgttct cgctcagatt ttgcataaca cacatcatag atattaaagc
    27181 ttaaagtctt agtcagatta ttgaatccat ataatttcat cttttcgttc acgtccacct
    27241 ccataataaa acaggacata ccattattct tgatacatcc atgtaaaaca gtcatgtcat
    27301 tttggtgatg cgtgcacagc cgctatccgt atcagagtct atatagcaag aactactttt
    27361 accactctta ttgaccgttt tacaagtgtg tatccaatac acttttggct tatagccatc
    27421 ttacaaaatc tgagctctta actcagtcaa taagcagctt tacgctgcaa tcggcagttg
    27481 acccggctgt ccgtctggcc ttagctcctt atataagaag cggtcaagaa gttcatgtag
    27541 tctgctaaat aaccgactca gaaaccgatg taaatataac attatgctat ggttatgtca
    27601 ataaaaaata tatacatcac tgacagccta tttatataat acacaaaaaa tctttaaaga
    27661 gaaaggagaa cagaaagata tctgttctcc ctgcatataa acggggcagt ttatatggga
    27721 attaagcttt tttatgttat ataacaaata atatcgttat cagtaaacca aatactattg
    27781 tcataattac agaaagtata attgctgttg tctttatgcc tattgcttta tttgacattt
    27841 atttacctcc tgtaaagatt acttcataac cttctaatac atatatacga aggtctgtac
    27901 atatattctg gggtagagct gaaaattatt aaaaaaaaac agaccagttc ttggtctgat
    27961 ctgttttcta aacgagagga tagattgatt gatattggtt ttctcccgtt attaatgaat
    28021 ttagacttcc ctgctattaa ctcattatcc atagtacata atacgaacag tatcatttaa
    28081 tttctggggt aggaaataaa aaaataaaaa aaagaccaga aaatttctgg tctaaattta
    28141 attaactatt ttattagtat catcccctgc cggatttacc ttgtcctgga gtctcttcag
    28201 gtcggcctgg agtgttatct gtcttaccaa catcagacat atcttcatca tcttctgatt
    28261 caatttcttc ttcagtttcc tctatttcct gctcatcttc taagtcgtct gcatcaggag
    28321 tttcctcagg tttgttttcc tgtcctggag tttcttcagg ttttcctggt gtgtcgtcag
    28381 gcttgttttc ctgccctgga gtttcctcag gctttcctgt tgtatcttca ggcttgtttt
    28441 cctgccctgg agtttcctca ggctttcctg gtgtatcttc aggcttgttt tcctgccctg
    28501 gagtctcttc aggtcctcct ggtgtgtctt caggcttatt ttcctgctct ggagtttcct
    28561 caggttttcc tggtgtgtct tcaggtttat tttcctgtcc tggagtttcc tcaggctttc
    28621 ctggtgtgtc ttcaggcttg ttgtcctgac ctggagtttc ctcaggcttt cctggtgtat
    28681 cttcaggctt attttcctgc tctggagttt cctcaggttt tcctggtgtg tcttcaggtt
    28741 tattttcctg tcctggagtt tcctcaggct ttcctggtgt atcttcaggc ttgttgtcct
    28801 gtcctggagt ttcctcaggt tttcctggtg tgtcttcagg cttgttgttt cctttattct
    28861 catttgtttt tgctacaatg tctttaactg acatctccag ccactcttgc ttatttatat
    28921 tttctggatc atcagaactt tcaataagct tttccactaa aacaagtttg cctggtgtta
    28981 cgccgagttc tctggcttct tccacccttt ctaacccaac tgcttctcca tatactatag
    29041 ctgaagaatt attcttttca acagcagttt ttactgattc ttgaagttta gctgccaatt
    29101 ctattgcatt attactgttt tgtgctgcag ttgatatcat aaccccagca tcttctccag
    29161 atagatatcc ttccacaacc gcctcttcta tagtcatatt taatgcttct tctattcttt
    29221 tgttctttaa gtcttttata tttaattgtt caagcaatct tattccgtct tcatttactc
    29281 cctcagcatc gataactctg ttaaacatat taacagaata ttccactgat ggatttatat
    29341 ccatacttac atatttagta ggtaaatagt atgaagatgt acctagtcct attgaaaaca
    29401 gcaaacttgc tgctgcaaga gcaattgttg ctttaatact cattattttt gttgtcattc
    29461 ctgcatcgac ctcctgtcct attgaatagt tcctattgcg aacctttatg atgcttccgt
    29521 catcacttaa catcgcggca gtattcttcc ttatatccac tataacagct ctcatatcat
    29581 ttacctcctt tcgttcctaa tatatgatac atactcagct aaatacggat aatcatcaga
    29641 taatagcttt gtagccgcta ttatatacat acgatgacgc tctaaaattt ttcggggtac
    29701 ctgtatatgc ataagtattt ttttaattgg aagttgtctt ttttcattta tttcattata
    29761 tatttccgga ttgtctaaaa tatagttaac aactaaagca catgctttct ttgtctttga
    29821 agacttaggt gaactttcag ccagagaata gaaagaaaaa ccaaactttg caaactcttc
    29881 attagcagca gaaatttcgt attttaacga ctcatctctg cttacagcaa gtttctcgga
    29941 taactgtgat tttatttgaa gattctcatc atcttcttca ggctctatgt cgtaaactga
    30001 cggatcaact gatatttcat tgttatattt tttttctgtc ctatagtaat cgattaattt
    30061 tctggttata agcaatttcg agtaagcata aaaagaacct ttttcttgtt cataagtttt
    30121 aactgcatca gaaaatgcaa taatggaaac agaccattca tcatcatgtt ccgatataaa
    30181 cttttttgtt acctcataag cgcattttat gatgaagcct ttgtttttta ttagtagttc
    30241 ttcaaaaacc ttctcatctt ttgctgcctg taacgctaaa tcatcgattg tattcataca
    30301 atcctccatc gctttacaat catattatac ataaatagtt atatactttc attgatttga
    30361 tatttttatt tgatcaaaaa aaattctgct aaaaatcaat attgtatttt gctcagtgat
    30421 atagtataaa gaatattact tatgcttttt tttcttaccg ctcaaaacgg gttgatgtaa
    30481 gtaaagaatc atttgcggat aacatgataa ggtttatgcg cactttaggt atctaattac
    30541 cataaatcaa tagaatgttt tgataaattt atagcgaacc ggtattttgg cttaatatgc
    30601 ctaaaaacaa ccaaaaatcg gaattgatat aactcttaac aagaagtaca attcattaaa
    30661 tttaattact ttggaggata ggaactcttg atggtagaac taagaaagat aacatgggaa
    30721 aattttaacg attgcctttc acttgatgtg acagatgatc agaaaaattt catagacagt
    30781 aatgtatatg ctcttgcaca ggcatatata gccgctacca acgataagtt tccacccatt
    30841 gcttatgcga tatataacga tcagacagta attggactag ttatactagt gcacaaatat
    30901 gctaaagaca gtaagtatta tcatgaaaac tgttatgaag tagtcagttt tatgattgga
    30961 aaacagtttc agggaaaagg ttacgggaaa gaagcttttg ccagagttat agatattatc
    31021 aagacatttc cagaaggaaa ggcaaaatcg gtctgtcttt catatgaccc tatgaatgaa
    31081 actgcacgac gattgtatgc ttcatacgga tttattgaaa caggttcagt aaatgacatt
    31141 ggcgaagtag tagcaaagct aatgataaat aaatagattt tttgcaaaga cggcttaaag
    31201 gccggttttt tacgattatc acattgacat atatcgatac aaaacataaa attagttatg
    31261 aacatggata taatcgaggt attgatatga aaaaaaaata cacacatgaa accgctatct
    31321 tcaaagcttt gagtgatgaa aacaggctgg agataatcga actgctttca aatggtgaaa
    31381 aatgcggatg tgttttattg gaaaaactgg acattcagca accaacttta tcacatcaca
    31441 tgaagattct ttgcgaaaca ggccttgtaa ataggtataa agctggaaaa tggattcact
    31501 attccctatc tgaaagtgca gcagaaacta taaaaagatt agccagactt tttgatgtag
    31561 cagaaaaaaa aaatgaacac agtaacatgt gtaaggaatg tgatacagaa aaaacataag
    31621 gagcggtata tggcacaata tagaatattg aaagatgcta agagagcagc aaaacagtta
    31681 gaagaagaga taatttcatt cggtaaagag atattaacca agtcaatgcc ggatttagat
    31741 aaaattaaaa gtctgcatga gtgggaaatc gaaaaagaca gaattaaatc agttattaga
    31801 aatgcatttc cctctgtttt atttaacaga gatgacttaa acgtaaagca tgtatcatca
    31861 catgaattta aaaatttcaa agtagaaaat gttctattta attcaattct tggctggcag
    31921 gtaaatgcat cagtgtatat accaaaagga gaaggtcctt ttcctgctgt tatttgccct
    31981 acagggcaca gttcaaaatt taaagaaaat tactatattc cagctcagat attcgcttca
    32041 aatggatata tagctgtatc attctgtcct ccgggttgtg ttggggaaca tgcttacagg
    32101 aatgatcatt ttgttaatgg ttattcaggt tggcttaacg gaatgtggag tcagacatat
    32161 tttatagctg atgcactagc ctgtgtagat tatgtttaca gccttgatta ttgtgataca
    32221 tccaataagg tatctataac aggagtttca ggaggcggaa ctacatcaat gtacacagcc
    32281 atacttgatg acagaatatc ttttttagct cctgtgtgct gcgtatctga caatataaat
    32341 gctcaattta aagacctata tacagcctgt cctgaaactt tcggaaaagg acttgctcgt
    32401 catggaatag aaacaacata tatcttagcc ttattctctc caaaaaggct atttgttgta
    32461 ggcggtgagc aagatgaggt atttgattac aaggtcactc agaaagttgt tgacaaacta
    32521 aaatatatat attcactata tggtaaagat tccaatatca attcatattt tgaaaaaggt
    32581 accggtcata aatatagcca gacaatggct ttagaagttc taaagcagat gaatgttata
    32641 caaaaaagga ctgatattcc tatattactt aatcgtgatg aaatagttca tcttgaaaga
    32701 gaacaactat cctgttatcc atctgctgaa gtaaatatgc ataccataaa cagagacatc
    32761 gcattacaac ttaaaaaaga cagacctgaa ttaaataaag aagaaactgt acaagttatt
    32821 aaaaaaatac ttgatataga aaatatgcag acaatggcag aacacaccta tatgtttcct
    32881 ttgaaaaatg ttccatcaag atggtgtcat aaattcagag acgttgtact gacccattca
    32941 aatgggaaaa aaataccttt aatccatgct ttcagagatg attatgtaaa aagacctgta
    33001 tttttatggt ttggcgaaac tgatacatgg gaacctttta ataggaaagg tcctctgaca
    33061 aaaatcattg gttttcttga aaggacacca gtcaagaatg agcattcagt aatgtcagta
    33121 aatatttcag gtctgggaga attagatatg cagcatacta catatgatgc ggcatcatgg
    33181 aacaggattg agagaattct tacatacttt tcggtattca atgaccggcc tataatggca
    33241 tataggattc gggatatact tatagcaatt aaccatgcaa aaaagtttac aaacgaaatt
    33301 tcaattggag ggtatggtat tggtgcaata agcgcaatat ttgcagcatt tattggcaat
    33361 gaagatatat caaaagtaat agctgtatcg ccaataggtt cgtatgaaat gattgcaaca
    33421 gaaccatctt ttaactggcc tgagagtatt atgatgccag atattttgaa atatacagac
    33481 ttgccttcag tacttggaca tcttggcaga aaagctgtat gcataaatcc caaagacgca
    33541 tacatgaaca aactggaaag cacttcatct aatgaaatat ttgataattc tatagcaaat
    33601 ggcgctaaaa tattatatga tgacgattgg gaaaatgccc ttgtaaaaga gatatgcagc
    33661 gataattaat tcaatctttt agcggaggat ataaatgata aaatatatag tatataagac
    33721 taaaaccgac ttcacaaaaa gatatgctgc tcttttatca gccaaattta aaattccata
    33781 tttatcaatg gaagaagcaa agaagaaact tatttctggt gaatccatat tgtatatggc
    33841 tggaataaga gcatctaaac tatatgacat aaaaaaagct ttagcaaaat acaatgttga
    33901 agcagttgtt gctgttggaa tgagcgctcc ctcagatgag tataataacg agataaaaaa
    33961 cagtaataat attaaagata ttccattatt ctatttaaga ggaggtactg ctcctgataa
    34021 gcttaccggt tttgataaat tcttgttcaa tgcaattgct aaaatgacag aaaagtcaaa
    34081 acaaaaagct gataagactg ctgacttgtc taaaaaagag gaaatacagc ttactgaatc
    34141 attttttaaa ggaatagatt atgtagcagt tgaaaatctt gattcaataa tagactggta
    34201 cagcagttat ctcataattc aagatatgat tagtaagaaa cataaaaaat aaattacaca
    34261 ttttccaact agtttcgcta caggttaatt ttattatctt cctttaaaaa tctaagctgt
    34321 tcctttggta tatcaaaacc taaagtgcca cctggattca taatattatc tttatcaaaa
    34381 tgctctttga gtgccctgaa tactgcatat tcattcttac ctattgaacc ttcaagccat
    34441 ggtccgaaca tcttgccgat tccatgatgg tgactcatgg atgcgtttga tttttgaatt
    34501 gcatcaagaa tacctgcatg atactgtttg aattcttcag ggtcactcat tttagtaatg
    34561 aagatgaagt aaaggttagc tccttgcggg tatacatgag acatatgagt catacatact
    34621 gtgtcaggtc ttgatttgca gtattttcta acctgttcat gtacatatga catgtttgac
    34681 cagtttacag aacattccat agtatcagta actataccga aatcttgcaa agtatctctt
    34741 aaatatgggt cattaaaccg tcccttttcc cattctttag ttacctgtcc tgttaaattc
    34801 ataccattgt attttcttgc aacttttcga acattctttg cacagtttct tgaaaagccc
    34861 ttttcaccat tagtaaatcc taaaaacaga cacatttgtc cttctttaaa accttttaat
    34921 ccaaatagat gctttaacgg actttcaata acaccataca tcctcagcat aacttgggtt
    34981 tcttcaggat cggaaagtct gaaaactgaa ggataccccg cttcagactg cattatctct
    35041 cttgcagcat tttgcgctgt ttcccagtcg ctgaacatat atgaaaacct ctttattgtt
    35101 tcaggcatat atcgaaatac tcttaatgtt acatgtgtaa gtactccgaa tgctccttca
    35161 gaacccatca ttatctgtcc aaggtcaggt ccaactgctt ttcttggata tttgtctgtt
    35221 tcaattacac cagcaggagt cgcataagcc tgatgtataa cgatatcaga aatagtacca
    35281 taataggtag aattctgacc ggctcctctt gttacggtcc aaccaccaac agaggaatac
    35341 tcaaaacttt gcggaaaatg tccacatgta tatgctctct ttgcattgaa aagttttact
    35401 gcatcattaa gcatctgttc taatttaggc ccgctcattc ctgcctcgac tgttatggtc
    35461 tggtctgtct cattaaagca aacaacttta ttgaatctta gcctcatatc taaagatatt
    35521 ccgcctttca tacactcaac tcctcttgtt acagatgagc caccaccata tacatatact
    35581 ggaatcttgt tctcagatgc gtatctgact attttttcaa tctgttctcg tgtatcagga
    35641 tatataacgg catcaggaac attttcgaca attttatttc tcagccttaa tatgtcatac
    35701 attgttttcc catatgcgac tgataatctc gaatagtcgt ctgttttaac aaaaccttcg
    35761 cccacaatct ctgtgaatac agatatatgt ttttcttcaa gtgttattgg gatatcatat
    35821 ctcaccttat caaaccccaa atcctccttg tactcatgaa aatatgaatc atcaatatgg
    35881 aatatctgct tcattagttt aaaaagattc tcattaggcg gtttaatgcg ttttgggtct
    35941 ccccatttga acaccgatct gtacgaatct ttagaaaatt cttctctaac ccattttggt
    36001 tcaaatccac tatacttatg cgccataatg tcctccttgt atgctttttt tataaagcgg
    36061 tagtagtttt ccgaaaatct ttacaaactg agtttcaaat atctttaaat actttctgtg
    36121 tttatccata tccggcataa aaacgtgttt tatatgcacc atggattcta ttgcttcatt
    36181 aatatcctta aatatctttt ttgaagtaaa cgcaactatt gctgaaccta gagcacctgc
    36241 ttcattagtc tgtattctgt acaccggaag tccaaacatg tctgcagtta tctgacatat
    36301 ctgaggactt tttgagcccc ctcctgcaac atatatgctt ttgatgtttt tcctgcctct
    36361 cttttccatt gtacgtttcc cttcaaaaag tgcaaagttt ataccttcaa taattgatct
    36421 gtaaatatgc atttttgtag tcatatcaga aaaacctaca atagcgcctt tggccacagg
    36481 catagaaaca cctgcagtaa aataagggtt gcataaaagg ccatcacaac caataggtat
    36541 atccatcatt ttttcgttta ataaatcttc tgcaattaca cccgattcca aagcctgttt
    36601 tttttcctta tcacaaaact ggtctttaaa ccatgaaacc agccagtaac ctctgtatat
    36661 ctgaatttct ggattatagt aacctctaag tacagcgccg taagaaggca gaaatggcag
    36721 aggttccatg tactttttcg tcatgaactg aattgttgct gttgtcccga aactgatggc
    36781 agcctgatgt tctgatataa ctgacaagcc taaagtctca catcccttat ccgaaccagt
    36841 tgcaataaca ggcaaccctt cttttatccc taaataatct gaagcttttt tagttatatt
    36901 gcctaatata tctccaggat caacaagatc acacatcaac tcatccttca cttcaaatat
    36961 acaccttgta atatcgtttt tcttcatcca gttacctctt ttattgtcaa aaggtatatg
    37021 acctattgaa gaagcttttg aatcagcaag attacctgta agaagatagt taagatatgc
    37081 agagattaaa acatatttat aggttttttc ccatatgtcc tgctgatgct cacaaatcca
    37141 gttgcaatac gatacttctc tttgcagctg tacagcttca ctcataccaa taactttgaa
    37201 tataattctg ttaagcaaag gaagtttttt attacatgta gcttttcttt tatcaagcca
    37261 taggataaca tctcttaaag gattgcacga gctgtccaag catatacttg tatccctgat
    37321 tgtagttata gttactgcaa ttatgtcctc ccagtgttcc ttggaactgc tttttaattc
    37381 attacaggtc agagccattg tatcccaata tacattaggt ttttgctctg cccatccagg
    37441 atttgtagag taatatgcct gttcatacga tttttgagat ttgttaatga tgttcccaaa
    37501 ctggtcaatc agcattactc tggtgctttg cgttcctata tcaaaagcca gaaccaaagg
    37561 tctattcatt gttatcccct tcttttaaag aatttgaaag atcacaagac gctataatat
    37621 tgcaccctgt tgctaatata tcaggtatta ttatatcgtg cattttaact ttttttgtta
    37681 ctacaatttt atttatttct tccattgcct taccaatcat atcttttggt aaatcatagt
    37741 ctgttctaac tggaaggact ggacaatttt taaatacagt tttaactgta gaagttaatg
    37801 accttttagg atcagtcatt tccagtattg caaattcctt tcctctttta caagcgttac
    37861 cttctaccgt tatttcttca ttattctttt ttactgtcat tacacatcct ctcggacaga
    37921 taatgcatat tatttgtttt tgcatttatt acaccttctc acatgaaaga aatatgttca
    37981 agatatcatt tccagctttt gataaatcca gttggattgt ctcaatttca gccggcctta
    38041 tttcaaccag tttctttgta gcaataaccc tgttatcagc agtcactgat attcttacat
    38101 ccttaattct tgctttgctt ctgaaatata catctacatt cttctgttca gataatttgt
    38161 ctatatactg aggaacacaa tatgaaagca gatcagattt ctgtaattta actaattttc
    38221 tttccttatt aatataattt gcagcacatc tgcctgctgt agttccagct tttgagacat
    38281 aatcaaccaa atcatttaca tgatgagcat taccgcatat aaatataccg tcttttaaag
    38341 tctgattaca ctggtcagct atgggacctt tggtaaattc atcaattggg acttccagtt
    38401 tttcagcaag ttcgttttcc ggtatcaatc ctactgaaag aataagagca tcgcagtcta
    38461 ctattttttg agttccctct ataactgaca tgtcatcatt aactgaactt atttcaactg
    38521 ctgtaagtct tttctctcca aaaacccttg taactgtttt atttgtataa agagggatat
    38581 tgtagtcaac aagacactga gagtaattac gcataagtcc cccaagttct gattttactt
    38641 catatactcc ctccacttgt gcgccttcca aagttaatct tcttgccatt atcatcccta
    38701 tatcgccact tcccagtata acgcattttt ttgttggcac atgtcctaga atatttgtat
    38761 agtattgtgc agttcctgca gtaaataccc ctgatggcct ggtaccatga atgaaaattt
    38821 gtttagcggt tctttccctg cagccagttg caagtattat agttttagat agtatatgct
    38881 ttataccttt ttgtgtaatc attgttatat gacaaaatcc atcttgattt ttttttacat
    38941 cagttacata tgttaaaagg cttatatcta tactggtttt attaatcata tcaatatatc
    39001 tctgagcata ctcagggccg gtaagttttt ctttgaaaat caaaagaccg aacccatcat
    39061 ggatacattg ttttagtatt ccgcctaacc ttgcttcacg ctctatcaag agagtttttg
    39121 caccattttc atatgcgctt tttgcagctc cgagaccagc aggaccagca cctataactg
    39181 ttacatcgta tgttttcata tcctgatgca tgctaaaccc ctttcctgct ttcataaaga
    39241 ataaggcttt cattatcttt tttagtcact tcttttagag caatattctt ttccttcgct
    39301 atcgtctgta aaacaagcgg accacaaaaa cctccctggc atcttcccat tcctgccctg
    39361 actctccttt taacgccatc tactgtatca cagggcacag atctgtttat tgcctgtttt
    39421 atctgtccaa gagtaatttc ttcacacctg catattacaa taccaaaatc aggattctgt
    39481 ccaattaata tatttttctc atcctcattc atattcttaa tatctaaaat ggcttttctt
    39541 attggattaa aatctttatt ttcctttatt tttatatctt cacttagaat atctttggtc
    39601 atttttgcta tatctaatgc tattgctgga gcagcagtca gtcccggtga ttgaattcct
    39661 gctgcgtgaa taatattttt ggttcttttt cctttggata taacgaaatc ctcctcgtat
    39721 gtagccgccc ttatacctga atagtatgca attacatcag atttttttat acctggtatt
    39781 gtagttctat gcttttctaa aatatcgttt atatttttat cttctgttga aaaatcctct
    39841 ctgtcaaaag tttcaaaagc atcaggtccg attagcagat taccatctgc tgttgtcagt
    39901 attcctcccc ctttactatg agtagtttta actgagctca taccataaac agaaactata
    39961 aaattgttat tttttactat cttcttatcg aaaattgcat ttgtaccttt tcttgcatgt
    40021 attgtaaaga agcggtcgtt tgccattgaa gcgattttat ctgaataaac tccagcagca
    40081 tttataacta ctttaggata aattgttcct ctgtttgttt ttaagcttat tattttattg
    40141 ctactgattt ccatacccgt aacagcagta tctaatgcta taactgcatt attatcaaca
    40201 gcattttctg catatgcaat aacaagttca taaggtgaca ctatacctgc tgttggaaaa
    40261 tatactccac cattaattgc attcgaaatt cctggaattt tttttcttag tttatcagca
    40321 gtcatgtatt ctgttggaat acccatagct ttccagtaaa gcctggcaat tgaaaatatt
    40381 aacttatagt atttcttgtt aaaacataga tactgcccag gtcttgaaaa ccgtacatca
    40441 agatccttac aaatctgatc atacatggca tttcctcttg aattgtattt ctgttttacc
    40501 tggcctttta aaagatcaat acctggatgt atctcgccat catttcttga tgaagcatgc
    40561 attcccacat catgttcttt atcaataagc ataactttta tatcatatct tgtaagctct
    40621 ctggcaatag ctgaaccaat tattcctgct ccaataacag ctacatcggt atgaatttta
    40681 tcgtatttaa gatcattaac tgaaggcatc ctcatttgtg gaatatcctg ttttgtatat
    40741 tcgatatcat ttaacacgcc aataaattta tttttattta cagctgtata tcccgctttg
    40801 actatatctt cccaacaatc aagagatcct gttaatttta cgcatccgtt ttcctctcgc
    40861 gcatgaacag aaccattgaa catcttattc agctttttat tcaacctgcg aataagaccc
    40921 ataaaaatac cgcctatatg aattataact cattttataa tatatttaaa tgtattagaa
    40981 tcttaaaagc ccgtatcttc tcggatcatg atcagtctca ccgcacatat atgttctttc
    41041 accgacaagt gcaccagacc agtaagctat aagctccctt ctgactccgc tgtcacgatc
    41101 actcagcaca acatcaaaac cccatatatc accaacagag ggtattcctc caatgcagtg
    41161 aaaaggtata tcgatacata catcataacc ttgaattgtc ttattcatac ttaatgatat
    41221 tgcactaaca tcttttaaac atttagtcat tggtatgatt ttggatacat ttccatttac
    41281 accacctttt aatatcattg aataaatacc atctgtaagt gtatctacat ttctgtaaca
    41341 ttgatctctt ctatcaatgt aaatctgaac acagtcatta tcgtaatcat attttccacc
    41401 gctgaaaaga acttcattat cttttatatg caacattata ctaagtgact gttctgatga
    41461 agtgaaaata ctcccctcaa cagaaagatc atcttttccc tgccaatgcc tttcagcacg
    41521 agaaaaattg gagctgtgat ttataaaaat gtttttctta tgatctgctt catttattgg
    41581 ttttgacctg tcatatttaa acacatcaat aattttcttt acaaaaggtt ctttatcttt
    41641 gttatataca accttcagat atccatcttg tattgatatt ccttttcctt ttagtggata
    41701 tctaaaagat ttgtattcat atggagataa tgtgactttc ccactgaaca gaagcgctgt
    41761 cttttcatag ctgttactat ttcttaagcc tatacacaaa ggtttatcat ctatactgtt
    41821 atagtctatc cagcattcgt atgaaatttc agaatcttct tcattttctt ttttaaatac
    41881 agcactttca gtattgccat catatgatat cttaacacct tctgatatat caatttccat
    41941 tactgaagag tcagaaaatg taaccaaata cttgtggtcg tttatggtgt tgaaacttac
    42001 tatatttttt gacttgatgt ccttttcatc aatatgctca taaacatttg caaatattac
    42061 ttttttaccc tctttttgct gaattagcag aggaaacagc ttaggtatgc tttttggagg
    42121 accatatgca tatgcactat actgaaccat ttctaaatcc gctgacgcat acatagacat
    42181 actggatggt ccatcagtaa atttattatt gtaaccgtct tttaccataa ttgtttttac
    42241 atcatgcaaa aaatcatatc cttcttcttc aaagatatcc tcaaaggaag tataatcctc
    42301 atcattcaat ttaccgaagc tgtgataaac attatgatat acatgatttt gatctgattc
    42361 tattaagaca taatcaaaaa ccatgtcatt atcaaaaaga atagcggttc ttctggctat
    42421 tgtgtttgga tatatctcat ctataattgc agatgctgca tcaaataatt tacctttata
    42481 gaaaaggtcg catttaccac taactgcaga ctgtctttta ccatcctgat taatagtatt
    42541 atggcccaat gtgcttctgt aatacctcaa gtgatcaaga taataattac ctgtaccgta
    42601 atctgtaaac catcttttac catgcaaaaa gcatgtaata ttaagtctgt caggatgccc
    42661 atgtcctccg ccataatggc catagtcaag attcagatag gaattttttc ttcttaagac
    42721 agcaagaccc gtacctgtca tgtttattga atctgttact ggaagtcctt tatcagaatg
    42781 agtttctggc tcaattgata aaaatcctct ccagtcgagt tcctctcttg tgccatatgc
    42841 tggtttcata atatccataa ttcctgcagc acttgataag ggcatgcttt tatcaggttt
    42901 catatcatat gtaaaattta gaaaatctgc tagagattta tctttgtaaa ctccatatgc
    42961 gagttcatat aacccacagt accatctgtt aactattgag ttcgcatatg gcgagtcttt
    43021 tcttgagggg aatgttccgt caggatgcag tgactttaaa ggtccataaa acatatcttt
    43081 caatgacctt ttattgaatg atttattaaa catgtcaata ccattataca tacacattgt
    43141 agcaatattt accattgctg gtactgttgc aaaatgataa ttatcccctt cataccacaa
    43201 accatcttca aggatggaat gccctatgtg tgctgcaaaa ccatgctttc cgtataaaga
    43261 ataatttaaa agatcatcgt cattagtcaa aaaagctaca gcacacatcc ctgaattatt
    43321 gaatgcctgt cggttattcc acgattcatc atagtctttt ataactacag ctgattcttt
    43381 aaataagttc agacattttt ttcttaattc atcagaaaaa gcagttgaat catttaccat
    43441 atcataacct gcgcatatgt agcacaacca tatcgattcc atgaaagttg attggaatat
    43501 ttttgtagta cctatctcat tatcttcatt tggataatga ggatagtata aaatatactt
    43561 ttcaagaatt tcttttattg catttgcgta tttattatca cttgttacta tatagcttat
    43621 gcctagataa accgacatct gagaaagcca tgaatgatag aagctgatcc aggcttttct
    43681 atatggctca tctttgaaat taactttgca tacagggcag atatagttat ctttgtcata
    43741 tgggtcaaat actaaggaaa cattatgagc tctgcatgca tcattaaaca catacccttt
    43801 ctgatctgga acagaaaacc ccttttctat catctcatca cacatcttct gaatccttgt
    43861 aaaggatttt ttataccatg gatatttttc taaatattct ctaatcttac ttattcttac
    43921 ttcatttaca aacagatgcg gttttgccat aacttatgac cttctttctt attctgactg
    43981 cttttattat atcattatac attggattta ttatggatat gtggtatatt tttagcataa
    44041 aggatgtgtt tgctaatgaa gaaatttagg cttgatgcat taatatttgt agtctttatt
    44101 gctttattcg gaataatgaa tcttctaaat ccggataaac ctacaatatc tttgcaggaa
    44161 aacagaaagc ttactgaaaa acccatattt acagttgagt cattgttcaa taagagttat
    44221 tttacaaatt ggaataatta ctattctgat accatattat atagagaaag tctgatacag
    44281 ctcaatgtaa acgcaaaaaa gaatttcggt cttggaaaag accagatatc catagtagtg
    44341 gttggaaacc agcctgctcc tacaaaaagc ccaaaaccta catctactcc gacacctgtt
    44401 gaatcagctg tacctactga tataaattca cctgttccta cggataatat accagaagat
    44461 acaaatactt cttcaccttc gcctgttcca acaggtacta atgaaccgat tgaggaagaa
    44521 ccgaccggtt atggtgaagc cacagggatg tatataactg taggtgatga aacattcaaa
    44581 ctggacagtt acagtgaaga ggcttatgac aactatgcca attacataaa taaaattaaa
    44641 cagcttgcag gtgaagatat tgaagtatac agcatgttag ctccttcacg ttcaacatac
    44701 cttgctctga caaaatatga ttttttaagt tcacgcgctg caaatgttga gcaaatcaac
    44761 agtaagattg attcctctgt aaagaatgtg aatgttttaa aaacaattgt agagcatgtt
    44821 gatgaataca tctattatcg cacagatcac cactggactc atcttggtgc atattacggt
    44881 tatactgaac tgatgaagac catgggtctt gaaaaagaaa tagtacctct tgaagaatat
    44941 gacgagaaac ttgtagtaga aggatatctt ggattttata acaaaccata tcttactcaa
    45001 gctgattatg acaatcctga taccataatc gcatattacc caacagttga ctatacatac
    45061 acataccatt cagcacaata cggaacagta gaaaaaaggc ttataaacaa agaaagagct
    45121 acacctgaaa aggattacta tcttctgttt tctgatggcg gatttggaac atatagtgtt
    45181 attactacct cagcagatac agataagtca tgtatggtta taaaggattc gtttggtgac
    45241 agtttaatat cttttcttct tcctcactat aaaacaatat atgttttaga ttcaagaacg
    45301 tacaacaagg aatattgcga caatctcaat atagtggatt ttgccaaatc aaaagaagtt
    45361 gacgatatta tcgtaaatta ctggattacc aatattacat atcttgatga ttttatggct
    45421 aaggcttatt caacacttga ttaaaattac ttaaataagg gttttggcaa aggtatgaga
    45481 actcttgtct gtttaatata ttcatcaaaa tctgttttat atccggcaag cctcttttct
    45541 gccattggta ttgatatgaa cacaaacatc aatgtattgg ctaatggccc tgctaaaaga
    45601 taaaacatat caggaacaga agacatcatc attatgaata taccccacca catcaaaatc
    45661 tctccaaaat aattgggatg tctgcagtac ttccataaac cttgtctcat taaagcacat
    45721 ctgtcgctat tgttttttcg aaattcatgc atctcataat ctgatatccc ctgtaataag
    45781 acagcaaaga aacacaccac aaatcctgct aatgttaaaa tattaaatga atcattctgt
    45841 attagtgcaa ttgcaggaag catacataag taaacaatta cagttggaaa tacctgaatc
    45901 ccaaaaaaat taaccagtgg aaatagccct tttgatcttt ctttcagcat atcataacgc
    45961 cagtcctgta tattaaggtt tttaaatgtc ttaacccagt tatatgtaag tcttactccc
    46021 catgttgtta ctgctgtaag cataaatatc gttcctaaac ctgtatttgc atattccata
    46081 gctaataaag gtaaaatcac tattggtgct acagaccaat atggatcata tactgtagaa
    46141 tttttcaaaa gaagactgaa taaataaaca gtaatagttg cagccatatc agctgtcaga
    46201 actttaatat atactgagcc tggagtaagc ctgtaaaccc atattccaaa aatacatgcg
    46261 ataacatata ttaatgctat aattgcaaac ccaataagcc tgttttcttt tatcttcatg
    46321 ctcaaatccc cctgcgcatg attatatcat atttgcaaaa tttccaaaat actaattagt
    46381 ttttnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    46441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnaatata aattagttaa
    46501 ttaaatataa attaattaat aaaaatataa attactattt gaacttatct agccagagaa
    46561 atttatttat tgctgtaatc cttttaataa cttatctctt tccaaataat ccggcatata
    46621 tttcgctact aaagaataaa atgattttcc atgatttctg tgtttaagat ggcacaattc
    46681 atgtacaacc acataatcaa ttgctttatc aggataaagc atcagtctgt atgaataaca
    46741 tataccgtta tcagcagtac agcttccaaa acgttttttt gctgatgtta tttttacata
    46801 cttcggatac aatcgtaata ttttcgaata atattcagtt tttctggtta aaacttcttc
    46861 tgcctgtttc ttaagttcca atatttcctt cttacttaaa cttttcaaat caggctgctt
    46921 tttttgtatc tcaatgtgct tatctatcca tttttcatgt ttggaaacaa aaccatcaat
    46981 catttttttt gatgcaaaaa gaggagctct cacaataaca tcgcaatctt gagatattcg
    47041 taatgatatc gttcttcttt ttgaccttat taaattataa tcatgcattt atgcatacct
    47101 ttttgtcaat ataatacata taaaaagtaa aaatcacttc aaaaacttga ctattgcaag
    47161 gatgactcct attaagctat atacccacac aagccatccc aataagccca taacccatcc
    47221 tagtaccgga ataggcccaa gcagtttttg cagaactccc ataacaatgg aaacaactac
    47281 atacaaaagt attgcaatta tcagtgagct gatgttcttc ttcttaacat tcttgttcaa
    47341 tgggaaaaat tgatccataa caatctcctt tattgatact atttataata taactgtatc
    47401 atacagtcac aataaaaaca acgagtttaa ccatttatac ttgtttaatc ttatatcaga
    47461 taatataatt gaatgaaatt gcaaggagta cgttaatgac actttgggaa cttttattaa
    47521 tagctgttgg attatccatg gatgcattcg cagtagctat atgcaaagga ttatccttaa
    47581 aaaagataac atggaaagaa gctctcacag taggcttgta ttttggtgga tttcaggctg
    47641 caatgccaat aatcggttat ttcatcggaa ctggtttttc atcatttata caaaaatggg
    47701 atcactggat agcttttatt cttttagcga taattgggtt caaaatgatt attgaaagtc
    47761 taaaaaaatc atgtgatatt aacgaagata cggattcttc taaatcttta agtttgaaaa
    47821 gaatgcttac tttagctatc ggcacatcaa ttgatgctct agccgtggga gttacttttg
    47881 catttttatc ggtaaatata atacctgcag tcagttttat agggataatt acattaattt
    47941 tatcctttgt aggagtatat ataggcaatt acttcggttg caaattcaaa tcaaaagcag
    48001 aattagctgg tggtataatc cttgtcctta tgggcactaa aatattgcta aaccatcttg
    48061 agataatatc attttaattt tttacataaa ctttgatttg gaatataata gtacttgcga
//
LOCUS       DAOSXM010000009        43975 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2111
            NODE_550_length_43975_cov_10.008903, whole genome shotgun sequence.
ACCESSION   DAOSXM010000009 DAOSXM010000000
VERSION     DAOSXM010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864178
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 43975)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 43975)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:24:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,261
            CDSs (total)                      :: 2,229
            Genes (coding)                    :: 2,218
            CDSs (with protein)               :: 2,218
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 3 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43975
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_550_length_43975_cov_10.008903"
                     /isolate="AMR_MDS_2111"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..902)
                     /locus_tag="PLQ68_02520"
     CDS             complement(<1..902)
                     /locus_tag="PLQ68_02520"
                     /inference="COORDINATES: protein motif:HMM:NF015736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HXK71555.1"
                     /translation="MTNSVKRIPPHNEEAEQYVLGCLLLDSSSFEPVQKRIDSSMFYN
                     KANQNVYISLQAVFMSGIPVDIISVSEQMKKDNTLNESGGVEYLNKLASQIVSSSSAE
                     YYASIIFDKFILRSLITVSAEVFEEGYKDSKDARELLEYSQQKLFDLSEQSDTQGVRK
                     ISDLIIQRMTYFREISKTKRMPERIETGFADLDKNILGFQPGDFVLIAARPSVGKSTL
                     AMNMAQNMAIRKGKKVLFLSLEMSGEQLTDRIIASEARVSNLKLQKGEADQNEWAKVT
                     ATAGVVHEAKIFIDDTSSITVADI"
     gene            complement(904..1353)
                     /gene="rplI"
                     /locus_tag="PLQ68_02525"
     CDS             complement(904..1353)
                     /gene="rplI"
                     /locus_tag="PLQ68_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HXK71556.1"
                     /translation="MKVILTQDVKTLGKKNQLINVSDGYARNFLFKKKLAIEADAKNL
                     NIMKDKIQSDNSKKDRELIEANILKSKLEDKEVKIEMLAGENGKLFGSITTKDIADAI
                     KKQHKTEIDKRKIHLDEPIKSIGTTNVAIKVHSDVSININIVVTEKK"
     assembly_gap    1492..1591
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1592..2413
                     /locus_tag="PLQ68_02530"
     CDS             <1592..2413
                     /locus_tag="PLQ68_02530"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HXK71557.1"
                     /translation="RFLESTLYPYEPIAILHESKDKKWLFVKMYNYEGWVQKEDVAYT
                     SFNILNLLINNHNFIVITEPKAYTKYSNRELDMGCTFPLLKKNNEYFTVLCPQKRADN
                     SLYFTFGYIPIEHGSINYLPYTQKNTLSQAYKFIDEPYGWGGMNGYRDCSALVMDIYR
                     SMGIKLKRNTDQQEIMSPINISVSKMSEADKINQLKTLPAGSLIFMNGHVMLYIGAYE
                     NTHYILHDTPGFYNDYGYNPVDSVTVTKVDIRNSQGYTYISQFTTFLYISEKDTE"
     gene            complement(2425..3240)
                     /locus_tag="PLQ68_02535"
     CDS             complement(2425..3240)
                     /locus_tag="PLQ68_02535"
                     /inference="COORDINATES: protein motif:HMM:NF019403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase
                     domain-containing protein"
                     /protein_id="HXK71558.1"
                     /translation="MTGFSTGLFFILKIYYYSYMKKGERQRLYLLDEIRGFLILGVVV
                     SHTLFDMYHIFGFDMPWVESALVNTISDIGAILFILISGMVSKYSKDNISRGLKLMAV
                     AVGLTLVTDVVVPQFVVYAGILHHLAFAIILIELLSPILKKIPATIAISINALIAVLT
                     WNIYEREISFFGLKVFDIPYEIMSGGLSHLLGLKIKDIIISSDYYPLLPWICFFVIGF
                     YLIDYVEREPIKTFFSKKRCLFLAKAGRVSLWIYIIHQPLVLGILYLITIIFR"
     gene            complement(3261..3614)
                     /gene="yajC"
                     /locus_tag="PLQ68_02540"
     CDS             complement(3261..3614)
                     /gene="yajC"
                     /locus_tag="PLQ68_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HXK71559.1"
                     /translation="MFNLILAETATETGGTNWTSLILIVVVFVVMYAVILIPQRKKDK
                     KLKQMLSELKTGDIICTIGGIVGNIVNVKDDEIVIETSIERTKMSMKKWAVRNVETVD
                     DSSTDSEPTGEIESK"
     gene            complement(3633..4760)
                     /gene="tgt"
                     /locus_tag="PLQ68_02545"
     CDS             complement(3633..4760)
                     /gene="tgt"
                     /locus_tag="PLQ68_02545"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002842759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HXK71560.1"
                     /translation="MNKYPVTYQHIHTCKQSGARLGIVHTPHGSFETPVFMPVGTAAT
                     VKGMTPRDLKEIGANIILSNTYHLYLRPSDELVAKAGGLHKFMNWDRSILTDSGGFQV
                     FSLNSLRKITEEGVAFRSHIDGSSHMFSPEKVMSIENNLGADIIMAFDECAPYPAERN
                     YIDDSLLRTTRWLERCIESHKRPNDQALFGIIQGGMYKDLRIKSAKQITSFDLPGYAI
                     GGLSVGEPANLMYEMLEYVTPIMPFDKPRYLMGVGTPDYLIEGSIRGIDMFDCVLPTR
                     IARNGTVMTSHGRVIVRDAKYSEDFTPMDPECDCYACKNFTKAYIRHLLKNDEILALN
                     LTTLHNLKFLLDLMAKIRQSIKEDRLLDFRDEFYEKYGYSK"
     gene            complement(4821..5849)
                     /gene="queA"
                     /locus_tag="PLQ68_02550"
     CDS             complement(4821..5849)
                     /gene="queA"
                     /locus_tag="PLQ68_02550"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005609249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HXK71561.1"
                     /translation="MKKSEFYYDLPQELIAQHPLDKRDSSRLLVLSKSSKKVEHKSFV
                     DILGYLNKGDCMVLNETKVIPARLIGHKENSTGKIEFLLLRRLNETDWEVLTRPGKRA
                     IPEKRFVFGDGILAAQVLEVVDFGNRIVRFEYEGIFEEILDKIGLVPLPPYITEELED
                     NDRYQTVYAKSSGSSAAPTAGLHFTKDLLEQIQKKGVDIVKVTLHVGIGTFRPVKSDN
                     IEDHKMHSEHYYISKESADFINAAKKEGRRIIACGTTSLRVLEGASDSNGMLCEKQGE
                     TDIFIYPGYTFKVVDNLITNFHLPESTLIMLVSALAGKDSVLDAYKQAIEKRYRFFSF
                     GDAMFIDN"
     gene            complement(5851..7443)
                     /locus_tag="PLQ68_02555"
     CDS             complement(5851..7443)
                     /locus_tag="PLQ68_02555"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HXK71562.1"
                     /translation="MNKKKRQRFSIILAMICLLSFSYANVANAVIYAPLYLQVGLFFG
                     DTAKQSYTISSPFGLNMTININNQEIDFASSDKTLVVAKDNGEYSYHIVRDDNYTSYK
                     EAYDMAVALSAAGASTFPVYSASGFWQVWLGSYASSDSAMNAIASVVARYPGSYSTIT
                     YPSSARILVRDKDTSRILFACDNPSAPVIAKTINTSNTSYMYIDNKPYRGNLEFTIFK
                     SSELCVLNNLLLEYYVYGVVALEIGSNSPIESLKAQAVVARTYALCQNKHQEYGFDVC
                     ATTCCQVYGGYKAETYRTNQATNETYGTVVTYNGKVVTPYYFSSSGGYTENSENVWVT
                     SVSYLKAVVDAYDINRTWSYSYSTQQMTDHMYKLGYRIGNVIKITIDSSSHTGRVLSM
                     TVNGSTGSATFLKSNVRNAFPDFLPSQMFVLGGQSTIKVANASGAATDQTLDYASIRG
                     IYNTSLISSKTIGVQTNLGIVTVSLMTDDTDKISISGRGSGHGVGMAQWGAINMADAG
                     YTYEQIIKYFYTGVMLESVTLR"
     gene            complement(7538..9142)
                     /locus_tag="PLQ68_02560"
     CDS             complement(7538..9142)
                     /locus_tag="PLQ68_02560"
                     /inference="COORDINATES: protein motif:HMM:NF028259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK71563.1"
                     /translation="MNSLFTLIKADLVNSYKLNGLKDPKNRTKAIGMAAIAFLVVAVC
                     FLYIGGASLIISDVLLQMQLLEVLLVFTFALPLFMLFFMDVYKMPSVLFSFSDYDMLM
                     SLPIKDTTVLAAKIIKLMLNGYLWQFLMGFPIFLVYCIKTPQLNILTIVLYVLLFLVI
                     PFLPMSIGSFVGLIVARISAKMRSKNVVIIIGSFVLLVLIMGISFSVQNLADKEALVD
                     AVSKISGIESSFFLFKMYVDSVARFNIVSLLLFLAIFVLPFILFIYLFGKSFKKINSS
                     MMETKAKHNIKNIKYNMDSPVIAIFKKELRAYTHCTIYIVNTFFGMVMLFVASIALLV
                     SDFDFNSVFSEFSELGFNPADFKMVAIALFFGFCIMMSSTTASSISLEGKNLWIIKSL
                     PVKPETIFMGKMMLGLAVTLPIGIISSFLVYIGIGLNFFEWFVIFILVIAYGFLSSVL
                     GLFMNLLFPKIEFKSPTEVVKQSASVGLTILAGMLLAVLPMIISPIVNVNVNLFILLV
                     SGVYALLTYMIWFFVKTKGVKKFQKL"
     gene            complement(9135..9848)
                     /locus_tag="PLQ68_02565"
     CDS             complement(9135..9848)
                     /locus_tag="PLQ68_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020816444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXK71564.1"
                     /translation="MLKIENLSKTYANNIKAVDNISFEIKSGDMFGFIGHNGAGKTTT
                     LKCVAGILEFEQGEIFINGKNVKKDPVGCKSDIAYIPDNPDVYESLTGIQYINFIADI
                     FKVSKHERERLTEHYAKQLTFTEALGDLISSYSHGMKQKLVIISALVHSPKLLILDEP
                     FTGLDPQAAFTVKEIMKNLCKEGGAVFFSTHVLDVAEKICNKVAIIKKGKLVALGDIQ
                     TVIKDRSLENTFMELAINE"
     gene            complement(9835..10497)
                     /locus_tag="PLQ68_02570"
     CDS             complement(9835..10497)
                     /locus_tag="PLQ68_02570"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HXK71565.1"
                     /translation="MARINVNPTRMELNNLKKRVVTARRGHKLLKDKRDELVKQFMNI
                     VKENKKLREICERMLGEAQNDFMLSRASISDDIIEESLMYPKNRIRLDVQTRNIMGVK
                     VPVITNIDDTEKDPNIYPYGILSTAAELDSALYNITEVLPYLIRLAEKEKTVQLMASE
                     IERTRRRVNALEYVMIPQLEETIKYITMKLDENERGNTSRLMKVKDMMIKEVRSKKDA
                     QN"
     gene            complement(10484..11881)
                     /locus_tag="PLQ68_02575"
     CDS             complement(10484..11881)
                     /locus_tag="PLQ68_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009080076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HXK71566.1"
                     /translation="MIKEYRTINEVYGPLMIVKDVENVKYNELGEIELSNGEVRKCKV
                     LEIDGTNAVVQLFESSAGINLADSKVRFLGKSLDLPVSKDMLGRVFDGMGNPMDKGPD
                     IIPEKRVDVNGEPMNPVARNYPSEFIQTGISAIDTLNTLVRGQKLPIFSGSGLPHPEL
                     AAQIARQARVLHTDTNFVVVFATMGITFEESDFFIRDFKKTGAIDRTVMFINLANDPP
                     IERISTPRMALTAAEYLAFEQDMHVLVILTDITYYADALREVSAAKKEVPGRRGYPGY
                     LYTDLATMYERAGRRKGSEGSITLIPILSMPEDDKTHPIPDLTGYITEGQIILSRELN
                     KKSIMPPIDVMPSLSRLKDKGIGKDKTREDHSDVMNQLFAAYSRGREVRELAIILGEA
                     ALTETDKLYAKFTDSFENIYINQGFYENRTIEESLDLGWELLVKLPRSEMKRVRDSNL
                     EKYYDKFKELDDGQD"
     gene            complement(11884..13650)
                     /locus_tag="PLQ68_02580"
     CDS             complement(11884..13650)
                     /locus_tag="PLQ68_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HXK71567.1"
                     /translation="MNKGTIIKVSGPLIVAGDMKHANMFDVVKVSEYGLIGEIIEMRD
                     DKASIQVYEETSGLGPGESVETTGMPLSVELAPGIIGGIFDGIQRPLEDMRKISGNTI
                     KRGINVKSLDREKKWDFKPVKKVGDVVQPGDILGLIQETVLIEHRVMVPHNVNGKITD
                     IYEGSFNITETIAKIENDKNEIIDVCMLSKWPVRRQRPFKMKYAPEEPLVTGQRSIDT
                     FFPITKGGIAAVPGPFGSGKTVVQHQLAKWSDADIVVYIGCGERGNEMTDVLMEFPQL
                     MDPRSNEPLMKRTVLIANTSDMPVAAREASIYTGITIAEYYRDMGYDVAIMADSTSRW
                     AEALREMSARLEEMPGEEGYPAYLGSRLAQFYERAGKVMTLGAEDRNGSITAIGAVSP
                     PGGDLSEPVTQATMRIVKVFWSLDSSLAYARHFPAINWLTSYSLYSSKLELWSDENIA
                     KGYSKLTQDAMRLLQEEANLQEIVRLVGIDALSKVERLVLEAARSLREDFLYQDAMHP
                     NDTYCSLNKRYKLLKTIMDFYYKSKEVIKKEIDIEELFELPVRERISRSRLIEESEIN
                     EHFRKIDDEMERQLKNLSEVEA"
     gene            complement(13667..13981)
                     /locus_tag="PLQ68_02585"
     CDS             complement(13667..13981)
                     /locus_tag="PLQ68_02585"
                     /inference="COORDINATES: protein motif:HMM:NF014092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HXK71568.1"
                     /translation="MYKAAVIGRHDDILAYKAVGFTAYDVEEAEFRDIVRSLDKQEYA
                     VIFVTEEIYEKNAEVIDKYTDKVLPAFIAIPGMNGTTGIGMMNVRRCTERAIGVDILI
                     ND"
     gene            complement(13974..14924)
                     /locus_tag="PLQ68_02590"
     CDS             complement(13974..14924)
                     /locus_tag="PLQ68_02590"
                     /inference="COORDINATES: protein motif:HMM:NF014094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HXK71569.1"
                     /translation="MIDSTKYAYSVGRVRVAENFMMDEVKLLRLVDAQDISEAQKILI
                     ECRYKEDDDYMAMLDNEHASLYSYIRSIIPDLNIFNAFFFKYDTHNIKVLLKSEFLKK
                     PFDEIASEVGVFKYETLKKFIQERDFSQLPKVIADGIIESIEDFSKSNDPQLIDLIMD
                     QAYYTYFNESINKTHDAFLKEFTVIQTDLINLKAFMRIKKVFQKESFLSRTLISHGSI
                     TLNDFKRLYEADEDEFKKFLKDTRYASLFDRGDVEKNCDDYMTSFLKDNRYDPIGIAP
                     VIGFMLGKETEIKNARIVMMGKANRISKDKTKERLRMCYV"
     gene            complement(14924..15526)
                     /locus_tag="PLQ68_02595"
     CDS             complement(14924..15526)
                     /locus_tag="PLQ68_02595"
                     /inference="COORDINATES: protein motif:HMM:NF014093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033178 - proton-transporting two-sector
                     ATPase complex, catalytic domain [Evidence IEA];
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E family protein"
                     /protein_id="HXK71570.1"
                     /translation="MSGIDRIYDKIINDAKEQADKRLDEAKQKISVMKKAVKEQLDEK
                     KQEQIELAEKEAENIIKRAVANRNLEGKKQLLSVKQKVISKVFETAHVELMNMPEDDL
                     LKMYKNLIIGSLEKGKNEIMLNEDDAKAIGQKLIGLLEKDIDSNFRVSLSSHFIQDDK
                     GVIVRNENIYSNSVFSSILKYKKQELETDIMKILFERNAK"
     gene            complement(15528..15995)
                     /locus_tag="PLQ68_02600"
     CDS             complement(15528..15995)
                     /locus_tag="PLQ68_02600"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HXK71571.1"
                     /translation="MLKYVLDNNLGDVLAILGAAVGFLLAAAGSAKGVSTAGQVGSGV
                     VAEDSSKFVPVMILEALPSTQGIYGFVVAFMIIGKLGGLNLADGMSLMAAALPAGVVG
                     LISGIFQGKVAAASIGIVAKRSNDMVKGVVLTLMVELFAIFGLLVSILMIGNV"
     gene            complement(16014..17984)
                     /locus_tag="PLQ68_02605"
     CDS             complement(16014..17984)
                     /locus_tag="PLQ68_02605"
                     /inference="COORDINATES: protein motif:HMM:NF013647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HXK71572.1"
                     /translation="MAVVDMQKISITGLFKDKKDILSFLMKRGVVDIQDMPIETEYED
                     VFTKGYDRNIESGINSDMSMISKAIEILELYDKTKKPLFKVRQEIDQQYYQDILNNES
                     KHIKFAEDVIKAESSIIDIKSEINSLENECQSLLPWIKMDIPVNLSKTDKVKIVFGAI
                     PEFVKPEVYLQHIDEQEFLCQIEQISKDRNNSYLVVYLHQSDTERPIHYLKSLGFNIL
                     EYDFTGKSSDILAEKKKEIENYKEQISTAKKQLDEYALKIDDLKAIYDCFAIRKGLLV
                     TDEKILSGKYSFIIDGWLPKEVSDKTAEELDKRFLCNVELREPYDDEEFPVLLKNGFL
                     GEAVSGVTEMFSLPNCREADPNPITSIFFALFFGMMLSDAGYGLVMVIACSIILLKFK
                     LEFSTSRFIKLMLISGVATMIVGALYGSWFGNLIPTLFSDTSIDIALWFDPIKDPNKL
                     LMWSLILGVLHIYVGYGIKGYNNIKRKKYLDAVLDVIPWYFFFTTAIFAVSSYIPSVP
                     ENIYTTLTPIGTSLLPYSAIVILLTQGRHKKGIFGKLFGGIASFYDLISFMGDVLSYS
                     RLLAMGLATGVIATIVNQLGSSGGFTIVGVLSFGLIFVIGHSFNFAINALGAYVHSSR
                     LQYIEFFNKFFEGGGVAFSPLLEDTRYIKIKE"
     gene            complement(17971..18297)
                     /locus_tag="PLQ68_02610"
     CDS             complement(17971..18297)
                     /locus_tag="PLQ68_02610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK71573.1"
                     /translation="MEKTYLDRIKQAEEQASDILRDAMHEAEYMISDAEKQNKEYEKK
                     LLEDIDNQYKNIIIQAEEKAKSIISKADLDYANQCEKLEKSAEPYIKEAVKYIKEKVT
                     NTDGSR"
     gene            complement(18446..18521)
                     /locus_tag="PLQ68_02615"
     tRNA            complement(18446..18521)
                     /locus_tag="PLQ68_02615"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18486..18488),aa:Trp,seq:cca)
     gene            complement(18589..19284)
                     /locus_tag="PLQ68_02620"
     CDS             complement(18589..19284)
                     /locus_tag="PLQ68_02620"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HXK71574.1"
                     /translation="MSLNEKVIRGIIFDMDGVLINSEQLMSKSAILALREFGVEAQPQ
                     DFIPYMGQGENSFLGNVARKYNVEYQYAMKYRAYEIYDELVCTEAELFPHIKETLNEL
                     RKRGYRISLASGADLMKVTSNIKAIEMEEEDFNAFVTGNDVTRNKPEPDIFLLAAERM
                     KLNPAYCLVCEDTISGIKAAKSAGMQCLGITSSLQSEVLKNSGADMTSESIHVLLELL
                     APLRGTTNRRKVV"
     gene            complement(19299..19670)
                     /locus_tag="PLQ68_02625"
     CDS             complement(19299..19670)
                     /locus_tag="PLQ68_02625"
                     /inference="COORDINATES: protein motif:HMM:NF012417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HXK71575.1"
                     /translation="MSEERLYMLFKKEEQKVSYKENMRGGQGTIMFNELLPEKMLNNK
                     GRLFSTITIEKGCSIGFHVHEKEAEIFYVIEGSLTYNQNNEKKYIISKGDCIYNKEGT
                     GHGVINESDEKAVLLACILFV"
     gene            19801..21180
                     /locus_tag="PLQ68_02630"
     CDS             19801..21180
                     /locus_tag="PLQ68_02630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK71576.1"
                     /translation="MSKYVFADWSVVEPGYGTVWPYSKSIVISPYGIRIEPHKPYIEK
                     DPVILPDKPWESSCVNSYATFLKADGKIKVWYETFSDAGMDDMKSNLAYAESEDGIHF
                     TKPKLNLIPFNGDYNNNLVKNVHGTCVLYDKTAEKKERYKLIWVSYDRTKPFNLSCTL
                     HGAVSEDGITWNELNEVLMNNPGDTQNILEHDEDLKKYVIYTRQIKHYPIPRRSVSRT
                     VSSSFNTFPEPDMILNNDPCDPPDWDYYTSGYHKWPGASNAHLMLMDIYKRTSDTFDV
                     HLLTSRDGIVWYRPMGLQPYIDKNSYGCKKDYMVSSTKGIIDNMDGTWSFYINSIQNG
                     HNIPPEERIKPAGGYRRAVIREDGFMSLTADSLGCFYTIKLDITQNFLMLNAEIPYGG
                     WIKVSIVDKDSCKPIDGFDEDSCDPIDSKKTWNSISWKNISDLSSLKNGTYRLKFTLF
                     KSKIYAFSI"
     gene            complement(21162..21890)
                     /locus_tag="PLQ68_02635"
     CDS             complement(21162..21890)
                     /locus_tag="PLQ68_02635"
                     /inference="COORDINATES: protein motif:HMM:TIGR00478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HXK71577.1"
                     /translation="MNRLDLYLTDKSLTKSRTSAKQHIKNGYVKVNGNIITKPSFLVD
                     YDKDKIEINNDEKYVSRGAYKLLQAFSDFKINVNGLVAVDVGSSTGGFTQVLLEKKAK
                     KVYAVDVGSNQLDDTLRNNERVISIENTDIRNIDKTVFIESIDFVTCDVSFISLEKTA
                     LSIYNLMNDGAKAVFLIKPQFEAGRTNIGKNGVVKDKAVRLQCVENIINHLEEIGFTV
                     IKTVQCDTAGKSGNVEYLCYIEKA"
     gene            complement(21883..23745)
                     /gene="dxs"
                     /locus_tag="PLQ68_02640"
     CDS             complement(21883..23745)
                     /gene="dxs"
                     /locus_tag="PLQ68_02640"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF003933.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HXK71578.1"
                     /translation="MIGTNDKMEVLDRIKVSGDVKDIEKENIDKLCIQIRKLLIDVVT
                     MNGGHLASNLGIVELTVALHRCLDLEKDKIVFDVGHQSYVHKILTGRKDCMVTMGNKG
                     GLNCFPDECESIYDIFNTGHASTSISAALGIEKSMKLKGIEGQVVAIIGDGALAGGMV
                     YEALNNASDLDSDILIILNDNEMSISRNVGAMSRYLSKLRLRPSYIETNTKVKNLVKK
                     LPNGGKGLIKLIQKTKRALKSYVGQKMIFEDLDIRYIGPVDGHDEQALEEIINETKKV
                     KGPKLLHVITTKGKGYDIAEKDPVKFHGVKNSLAKRKRLSANPMYNEYFGRLISDMAK
                     KDENIVCVCPAMTEGSGLYEFSINHKDRYFDVGICEQHAVTFSAGLSKNGLKPICVIY
                     STFLQRAYDQILHDVCMTNKKVVICVDRAGVTDAYGKTHQGIYDIAFLSTLPNMTILA
                     PESKDDMKKHLNESIYSLDGCIAIRYPYDEINSDISKQCDLDLGPLQCAVYNKGKDKA
                     ILCYGRMVPLALEAAKKSNASVVKIRKLYPIDNQQLYDLLKDYKYVICAEDVIYNGSV
                     AQRFESWMYKNNKKSFRFENLSIKEDCEMRGCVDDILADNNLTSDALSKLLEDE"
     gene            complement(23729..24598)
                     /locus_tag="PLQ68_02645"
     CDS             complement(23729..24598)
                     /locus_tag="PLQ68_02645"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HXK71579.1"
                     /translation="MTILEKTISTYGQSVNLFINEIINNSSAPDILKKACLYSINAGG
                     KRIRPALFLMITEALGIKISNKHIAFASALEMIHTYSLIHDDLPSMDNDDYRRGKLTS
                     HKKFSEAYAILAGDSLLNLAFETLLEYSDSKKDIKAALHIAKASGHEGMVGGQVMDMS
                     ALKNTDEVLKMYSLKTGALIKASIMSASVMAGLGKRDLKKLENYADNIGIAFQLRDDV
                     IDKTNINSNTGKPKNSDERNDKSTYISIKGFKANEKKLHKHTNNALRAIDTFGERFDM
                     LRQMAIYLDDRDK"
     gene            complement(24602..24802)
                     /gene="xseB"
                     /locus_tag="PLQ68_02650"
     CDS             complement(24602..24802)
                     /gene="xseB"
                     /locus_tag="PLQ68_02650"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016203266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HXK71580.1"
                     /translation="MSDKDFNELYKELEKIVEQLEKGELSLEEAVKTYTQGHELIKQL
                     NEKLEKAREKMVVTGKSELKEM"
     gene            complement(24795..26000)
                     /gene="xseA"
                     /locus_tag="PLQ68_02655"
     CDS             complement(24795..26000)
                     /gene="xseA"
                     /locus_tag="PLQ68_02655"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HXK71581.1"
                     /translation="MEKTLTVTELNNYVSQLLSSDDILESLKVEGEISNCTIHYSGHV
                     YFSIKDDNCSIRCVMFDRSARNLKFKPENGIKVYVYGYLSFYEKTGQTQIIVTVMTEG
                     GTGDLFVKFENLKKKLELEGLFSHENKKPLPYLPERIGIITSSTGAVLQDIRNTLQKR
                     FDNYGMILYPATVQGTDAHKTIIEGIRYFNDNNNVDVIIIARGGGSVEDLWPFNEEEL
                     AYAIYASEIPVISAIGHETDFTIADFTADARAATPTAAAQMVMPEKNMLIKDIFLKRD
                     SLNNAFSKLLRNLDDKILNIKQRPVMRRPEEYTNILGLNLDNITNRFFLASKAYIDGI
                     YSRYNILNQKIEALNPLNVLSRGYSITTDKDGKVIKCSSMVNKDDRIITKIFEGTISS
                     IVEKAEKNE"
     gene            complement(26000..26404)
                     /gene="nusB"
                     /locus_tag="PLQ68_02660"
     CDS             complement(26000..26404)
                     /gene="nusB"
                     /locus_tag="PLQ68_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HXK71582.1"
                     /translation="MGRRKTREHAFKLVYQIQINNDDINEQMEQYFMDNEVEENARQY
                     IVNAVNYINQNHQEIDSVISQYLAKNWRIERIPKVELAIMRLAYYEIMNLDDVPKSVA
                     INEAVELAKAYGSDDAGKYINAVLANVQDKDL"
     gene            complement(26419..26961)
                     /gene="amaP"
                     /locus_tag="PLQ68_02665"
     CDS             complement(26419..26961)
                     /gene="amaP"
                     /locus_tag="PLQ68_02665"
                     /inference="COORDINATES: protein motif:HMM:NF033218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HXK71583.1"
                     /translation="MKIFKKGKNFFLAINIILVLAICFFVIYACLDPLDFEKAFEDFV
                     DARFFSAQRGLLFLAALLVIGFEILFISSMIQKEEKPAETVSIETPSGQITIAVYSLE
                     ALAKMEASQFDMVSDIRTKVDMKDTQIGMDVYVKVMPTVLIPEISSKIQNAVKEKILS
                     GTGIDLHHVRVFVDGIAGIS"
     gene            complement(26948..27346)
                     /locus_tag="PLQ68_02670"
     CDS             complement(26948..27346)
                     /locus_tag="PLQ68_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006306754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HXK71584.1"
                     /translation="MGEDTLNISGLETPLGQLKVTNEVIAILSGIAAMEEDGVYAMSG
                     GITGELAQALGIKNLSKGVKIETDEEGIHVNIYVIVELGVRIPDVAWSIQEKVKKMVE
                     KMTGSKVAEVNIHVQGVNIFKGKENLNENI"
     gene            complement(27408..28505)
                     /locus_tag="PLQ68_02675"
     CDS             complement(27408..28505)
                     /locus_tag="PLQ68_02675"
                     /inference="COORDINATES: protein motif:HMM:NF014542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HXK71585.1"
                     /translation="MDKTKVAVIGGFLGSGKTTLILNLAKSLIKDGLSVGIVTNDQGS
                     DLIDAKFLSSNGLDVTSVKGKCFCCFFDDLASKLSEFRNRKAADILLLEPVGSAINLV
                     STIFKPFVNNRSVMLDSFNEGFILSPLVVLADPKRVRHLMMSEQKPEYTTPVSYLFSK
                     QLEEADLILINKKDTINEQEQADLKVFLESKYPNTEIKTISALDNDFVNDIKNFVLTG
                     SFVINKSIEVDYVKYTEVEASLSWLNSNAFVKFEEVKDINKLMDKFMNLVRDELIESK
                     SSIAHMKCYAVADEDFYKASITSNTDSISVNNQMRLEHDSFNIIINARIISSPITLKS
                     IFESNLKQIFNPKSINTESFSPKYPKPNYCE"
     gene            complement(28498..28713)
                     /locus_tag="PLQ68_02680"
     CDS             complement(28498..28713)
                     /locus_tag="PLQ68_02680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK71586.1"
                     /translation="MINLFERIKPEYPDVDFVITILERPEDARKVDGLEIDLFPAILI
                     DKEQITAGNILHKRQLKGYIDVRRPNG"
     gene            complement(28773..30131)
                     /locus_tag="PLQ68_02685"
     CDS             complement(28773..30131)
                     /locus_tag="PLQ68_02685"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HXK71587.1"
                     /translation="MNKKQLVLDAVTNKQTQRAPWVPFVGCHAASLLDNVTAEQFFKD
                     GDLIVKGVKKAVELYDPDGIPVLFDLQLEAEALGCKLRYADDNPPSVVTHPLEDGVKL
                     EDLKVPTINDGRFALVMKATKQLCDDLGNDIAMYGLITGPYTLALHLMGTEIFYEMID
                     DPDHVMEVLEFCNKVCIACADMYIDAGCDVIAVVDPMTSQISPDSFEEFVLPYVKQVF
                     NHISDRKKLSSLFVCGDAKKNIEVMSRCECDNISIDENIPLEYVIDICKKRNVSVGGN
                     IKLTVTMLFGSVQDNINDAMNCMAIGGTKGYILSPGCDMPYATPVDNVLAISAVAHGQ
                     MPESIVSQSSLDGIEVVIPDYKTEKKVIVDVVTLDSASCAPCQYMMEAVKAACAGLEE
                     DVEYVEHKIKDKESVVFMMKLGVSNIPTIVIDGEIRYISIVPSVKQLRENIEKAIELK
                     SK"
     gene            complement(30201..30680)
                     /locus_tag="PLQ68_02690"
     CDS             complement(30201..30680)
                     /locus_tag="PLQ68_02690"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXK71588.1"
                     /translation="MNEHNNLKIEKIDEDSFNTIKDLRDDIKDESQFFERIIKIKEKT
                     DECYAVMFEDKAIAEVTVTLKSADATVAKEGLRIYISGLYVKKAYRNLNIATYLISYI
                     VDTFTNYGYKEITVLVDRTNTAAVNLYKKMGFINERTYKEDDWIFDLLILKMKNTTK"
     gene            complement(30680..31756)
                     /locus_tag="PLQ68_02695"
     CDS             complement(30680..31756)
                     /locus_tag="PLQ68_02695"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HXK71589.1"
                     /translation="MIRTRINSLRKTLVEKNLEGAIITSRDNNMYLSEFSGTDSVIVI
                     TSDKAYFATDFRYIETASSLSSRGYEIILIEEGIKSIVELLNNIFTSKGLRIGFEKTM
                     SYGYFDSIKPALDGYVFVDIYDDLLKIRSVKDEVELERMSKAASIADLAFSHILKTVR
                     IGMSEIEIAAELEYVMKRNGASYPAFDTIAISGKKTSMPHGVPDNKKLQIHDAVTLDF
                     GANYKGYCSDMTRTFFMGQPSDELEKIYRIVYRAQKEAQDYVKEGISGYSGDKVARDI
                     IEKEGYGKYFGHSTGHGVGLLIHEEPRLSIKSKTVLKKNMAVTVEPGIYVPDLGGVRI
                     ENTVIVEQGGCTALNHSDKELIIL"
     gene            complement(31777..32619)
                     /locus_tag="PLQ68_02700"
     CDS             complement(31777..32619)
                     /locus_tag="PLQ68_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004625679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HXK71590.1"
                     /translation="MIRFGPAGNETSFYDQGYKNTYQAMQWLEDMGLNAYEYQCGRGV
                     NIKEDSANQIKEYAIKHNIALSLHAPYYINMATKEPQKVESSLMHMRKSYIAAKQMGA
                     TRICVHPGAVMKDTRENAAKRAYVFLKSFLDEINDESVIICPEVMGKINQLGNLEEII
                     DLCSKEERILPTVDFGHLNARTHGSLKSSDDFERVVVQMIDGMGIERTRKMHIHFSQI
                     EYTEGGEKKHLTFEDTTYGPYFKDFIPVLYKYDMQPVVICESDDKMATDALKSKIMYE
                     KYKQ"
     gene            complement(32616..33119)
                     /locus_tag="PLQ68_02705"
     CDS             complement(32616..33119)
                     /locus_tag="PLQ68_02705"
                     /inference="COORDINATES: protein motif:HMM:TIGR01668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HXK71591.1"
                     /translation="MQKYLPDYISDSIFDIDYDKLYDMGIRGLAFDIDNTLIEMNNDD
                     LHHKAISLIGSLKNKGFALCILSNASKKRTAMIGEMLEMEYIHRAFKPSQKGFIKARL
                     LMNLPKEKCVMIGDQMFTDVLGGNKAGFLTIMTKIIDNNEAFNIRIKRIFEKVVMKKF
                     ERKIKKI"
     gene            33209..34273
                     /locus_tag="PLQ68_02710"
     CDS             33209..34273
                     /locus_tag="PLQ68_02710"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020814586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HXK71592.1"
                     /translation="MNISIKLTQNPKKKPDEDKLSFGKQFTDHMFLMDYKEDKGWHDA
                     RIVPYGPLTLDPSTNVLHYGQGVFEGLKAYKTEDNRVLLFRPDENAKRLKSSCERLCI
                     PPIDEIDVVEGIIKLVKTDIDWVPSSKGTSLYIRPFIISTDPFLGVHPSHSYLFMIIL
                     SPVGPYYPSGLNPTSIYVEDFYKRASKGGMGHVKTMGNYAASLIAQQKAEKLNCIQTL
                     WLDAHEGKYIEEVGAMNVFFVIDNKVITPKLSGSILPGITRKSTLEIAKSLGFEVEEK
                     LISIDDVVSALEKGKLQECFGTGTAAVISPVGELIYKDTKYVINDNKIGQISQKLYDT
                     LTGMQYGKIEDTFGWTVQVK"
     gene            complement(34383..35435)
                     /locus_tag="PLQ68_02715"
     CDS             complement(34383..35435)
                     /locus_tag="PLQ68_02715"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF017509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HXK71593.1"
                     /translation="MYSKNLMKKCIFILMIVLILSVLVSCSSPYKNSKYITLSKYMGV
                     EVDYEEIKVTQDDLDREIAIFLDSYAEVVVFEDGIVQEKDIVNIDYSGTIDGKPFENG
                     AHTDYDLEIGSNTFIDDFEDELIGTQIGQTTEITAEFPTNFKDNNGNISDLAGKEAVF
                     IVTVNKVTRRVPPEYTDEFLGSLNIGYKTVREHNQFLQQEILRYNKIQTVWNIIVDST
                     EVKKYPNTVEKRVAMVREYYEYYMAYYGYTSFNEFCIDSLGQTEEEFNQTNLLSAQEG
                     VKQEMISQAIAQLEDINLTNKEIQDGYDNYAEYYGYDNTEDFLKEYDKESLQSSILLN
                     KIMEYVAQHAIIINNP"
     gene            complement(35457..>36005)
                     /gene="yihA"
                     /locus_tag="PLQ68_02720"
     CDS             complement(35457..>36005)
                     /gene="yihA"
                     /locus_tag="PLQ68_02720"
                     /inference="COORDINATES: protein motif:HMM:TIGR03598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HXK71594.1"
                     /translation="EVVFSGKSNVGKSSLINALCNRKNLAYSGSTPGKTRMINFYDIN
                     GELRFVDLPGYGYSKASKQIAKGYSNLADDYFKERKNLRLIIQILDIRHDPTDDDIQM
                     IHFLNKSEMPFVLVIGKCDKLSKAQSITRARKIAKMLIIDPDTPFILTSAITKRGIEE
                     IWDVIEYYCFYEEEAEKERQKE"
     assembly_gap    36006..36105
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<36106..36941)
                     /locus_tag="PLQ68_02725"
     CDS             complement(<36106..36941)
                     /locus_tag="PLQ68_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HXK71595.1"
                     /translation="MALITSKKMFEKAYKGGYAIGAFNVNNMEIIQGITEAAKEAQAP
                     LILQVSAGARKYAKHVYLMKLIEAAIEDTGLPICVHLDHGDSFEICKSCIDGGFTSVM
                     FDGSHHTFEDNIKETKRVVEYAHERGVVVEAELGRLAGVEDDIKVSKEDSSYTDPDEV
                     EEFVSKTGVDSLAIAIGTSHGAYKFQGEAKLRFDILEEVQRRLPGFPIVLHGASSVIP
                     EFVEKINKYGGKMPGAQGVPEEMLRKAASMAVCKINIDSDLRLAMTSSIREHFALNPD
                     HF"
     gene            complement(36954..38204)
                     /locus_tag="PLQ68_02730"
     CDS             complement(36954..38204)
                     /locus_tag="PLQ68_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014314183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HXK71596.1"
                     /translation="MTLLKGAAIFGQSGGPTAVINSSASGVFQQALKEECITAVYGAA
                     HGIRGILNEEFYDMSEETQEQLDLLKTTPSSALGSVRYKLKNAEVDETDYLRLLEVFK
                     KYNIRYFFYNGGNDSMDTCNKISKFLAKSGYECKVIGVPKTIDNDLSVTDHCPGYGSA
                     AKYVATTIMEVYHDANVYDTPMITIIEIMGRNAGWLTAASMLAAYKGAGPDLIYLPEV
                     PFTIDKFLKDVKKVYDSKPDHKVIVAFSEGCKDTEGRYIAEMGQSLAKDAFGHAQLGG
                     AASVLGDIVKNEFKCKVRSIEFSLMQRCAAHIGSRTDIDEAFLAGQEAVKAAVNGNTD
                     YMIGFERKINSKKYECKTTLVALSDAANTEKTVPLEWLNEDKNFLKQEFIDYALPLIQ
                     GDSGLPFEEGLPKFAKLKKVLVKK"
     gene            complement(38279..38548)
                     /locus_tag="PLQ68_02735"
     CDS             complement(38279..38548)
                     /locus_tag="PLQ68_02735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK71597.1"
                     /translation="MKNNQTNKRNVIDWVLIFLFIFLRFISDNQLYRNYALLAVAVLG
                     ILISLGILIFKKDLAKTGKIRELIAIGLLVIAVGYAVFRIITLSA"
     gene            complement(38545..39006)
                     /locus_tag="PLQ68_02740"
     CDS             complement(38545..39006)
                     /locus_tag="PLQ68_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011971165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HXK71598.1"
                     /translation="MLKKYVIIPALIAAIYCALIFMFMPVSFGTVQIRVAEALTVLPY
                     FTPSAIYGLFVGCFLANLIGGAGILDIVLGSLATLLAAFLTYRMKSKWLAPFPPIVIN
                     AFVVGYILNIYYGMDYWLAVASVAAGQTVSCLGLGMLLLLQLDKYKERLFS"
     gene            39246..40706
                     /locus_tag="PLQ68_02745"
     CDS             39246..40706
                     /locus_tag="PLQ68_02745"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HXK71599.1"
                     /translation="MNNLVSLFRSIDISGKIVIVVILLVFIAAFVINLLIKIQYYKLS
                     KQINNRQNRRAGTFKDEMLNEIVQDYKVAGEINNNNVNTQAIIEKNLTEHLKLSSFGE
                     TFVRKSMSMMITLGLLGTFIGLTISVSELVNVLLQDIGSASLDWNEILVRLAAAAKGM
                     GAAFTTSLVGLFGSVILNFALIAIDCEEQKRSLMIDVEEYLDNNVAVLIAKDKETEYT
                     MMNRILKDTFVEFGSKIEMTLKDTVDSFADKLTNVVMDVSVSSQALDTTVERFDSAIS
                     TLAVAMKDMSDFNVNLKENVDKMDVSFIKMSESLSDSANLIMKNYDAIRSFAEDVKSA
                     AGQMAVSNKETLEELASLAEQVDQTVSALQQLTTTMKQSSEDNAESINNMKDSFEKAI
                     IATSMEVSSLTEKIKSSFEEALNESSQIIAEKTAATMEKSMANVNSMSESFENNQKIL
                     AQTIASLPEQTMVYNKSVSGKIQKKLDDIEKAIRNE"
     gene            40730..41767
                     /locus_tag="PLQ68_02750"
     CDS             40730..41767
                     /locus_tag="PLQ68_02750"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HXK71600.1"
                     /translation="MKQRVSYLKKTEAENYWPSFADVMSTMVLVMLFLVLIVFIKNII
                     ISINLDEQEATNIATGAQLEATRNDLAAKELALQETQLDLETKQTILVMLENELAAKE
                     ISLSLLENELVAKQAALELSEEEAANLENLILILAEQQAALEAQKQELLSLIALNTIE
                     LESMREQLQKVAVMQVDIFEKVKKSIDEALGEGYVTIGDEGNLQINASLLFQVDSSDI
                     SPSAYPLLDKLSSAFEDILKDSGQYIESIMVEGHASSDGSYEKNQLLSTDRANSVLLY
                     LFGSNPKLETQYSSYFGAVGYSESRPLVENDYTETLRAKNRRIEFSIVVREENVGQII
                     QEYLDSLDDAS"
     gene            41776..42678
                     /locus_tag="PLQ68_02755"
     CDS             41776..42678
                     /locus_tag="PLQ68_02755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK71601.1"
                     /translation="MAKNEFKDLKLYYSNSMISLKDGDYDEAIKGFKYLIKHGIETQK
                     SVLGLITAYSCVTRYNNALKIYEEHKEFFTGKTPYKGMFVEIMTALLIKESTLLKKNT
                     RGYLTGIITARRMKEVHEAYLANPDNLLCIILICYWYAVIAKRPKDTEQMMMKFVNDE
                     HIEDEFRWKLLEKLAITDKQIMEDITIAGKFKRIPRYLDHSYVNLLLFSSLSSNNLII
                     ARENIEVQRMNGVQLNDDVMWNYLDLCVENDDIDDLAVNFAKRLFSKGWMDPVIAKVL
                     RYAKDNLNIYNVKNEMKSLELFGI"
     gene            complement(42698..43249)
                     /locus_tag="PLQ68_02760"
     CDS             complement(42698..43249)
                     /locus_tag="PLQ68_02760"
                     /inference="COORDINATES: protein motif:HMM:NF013624.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK71602.1"
                     /translation="MKVLVSGFIAFAGFSENITEKIVNSLPVSLNNIDIYTVILPVEY
                     ENAFKILDNMIDQIKPDFVIATGMAASRKFISIEKIAVNIDNSDTADNAGVIKKDSLI
                     VTNEDIAKTASLEYPDKVYEIEGVKNSYSAGTYVCNDLFYRLLSKSDSKDYPKISFIH
                     FPSEDNMPYEKQLSIFLSILSCL"
     gene            complement(43246..>43975)
                     /locus_tag="PLQ68_02765"
     CDS             complement(43246..>43975)
                     /locus_tag="PLQ68_02765"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXK71603.1"
                     /translation="NYNGGLATYLTKGPIPFESALEYKKAYPEKFVLGYCPDPRNPDA
                     INNLKAAVYIHNVQVCGELKVRMMYDDYDAIRFYRAAGELNLPVIMHFDYEFNKPWSN
                     SWPNFWYGGGIDVLERILKKCPETTFLGHAPGFWAHISNDDQFDKLSYPKGPVIKGGE
                     VSRLLDKYDNLYCDMSAGSGWNAFNRDLEHAYWFMNNYQDRILYARDYFDNRHQELIE
                     SLDLKQEVKDKIYFKNALKLVPLK"
BASE COUNT        13755 a         8419 c         6327 g        15274 t
ORIGIN      
        1 cttatatctg caacagttat tgatgatgta tcatcaataa atattttagc ttcatgaaca
       61 acacctgcag tagctgttac tttagcccat tcattctgat ctgcttcacc tttttgaagt
      121 tttagatttg atactctcgc ttctgaagca ataatcctat cagtaagctg ttcaccagac
      181 atttcaagac ttaaaaataa caccttctta ccttttctga tagccatgtt ctgagccata
      241 ttcattgcca aagttgattt tcctacagaa ggtcttgctg ctatcaatac gaaatcccca
      301 ggttgaaaac ctaaaatatt tttatcaagg tctgcaaatc ctgtttcaat cctttcaggc
      361 attctttttg ttttacttat ttctctaaaa tatgtcatgc gttgtataat aaggtcacta
      421 attttcctta caccctgtgt gtcagattgt tcagataaat caaaaagttt ttgttgtgaa
      481 tattctaaaa gttcccttgc gtcttttgaa tccttatacc cttcttcaaa gacttcagca
      541 cttacagtaa ttaaagatct gagtataaat ttatcaaata taattgaagc ataatattca
      601 gcactggatg atgatactat ctgtgatgcc agtttgttga gatattcaac tccccctgac
      661 tcatttagtg tattatcttt cttcatctgc tcggaaacag atattatatc aacaggtatt
      721 ccactcataa aaactgcctg caaacttata tacacattct gatttgcttt attatagaac
      781 atagatgagt caattctttt ttggacaggt tcaaaagaag agctgtctag aagtaagcat
      841 cctaatacat actgctcagc ttcttcattg tgtggaggaa tcctttttac actgttagtc
      901 attttatttt ttctccgtaa caacaatatt tatatttatg cttacatcac tatgaacttt
      961 gatagcaaca tttgtcgtgc caatgctctt tatgggttcg tcaagatgaa tttttctttt
     1021 atcaatttca gtcttgtgtt gttttttaat agcatcagca atatctttgg ttgtaataga
     1081 accaaaaagt tttccgtttt ctccagctag catctctatc ttaacttctt tatcttcaag
     1141 tttagatttt aatatattgg cttctataag ctctctatct tttttgctat tatctgactg
     1201 aattttatcc ttcataatat tcagattctt tgcatcagct tcaatagcta actttttttt
     1261 aaacagaaaa tttcttgcat atccgtcact gacattaatt aattgattct tttttcctaa
     1321 ggttttaaca tcctgtgtaa gtattacttt cataatttcc tccgaattag tcagtcattt
     1381 aatgttatta taaaccacaa taacacgttt tatataaaaa catcatttgc gatgttataa
     1441 tcataacatg aaaaaggttt tatctgcaat attaacactt atcatattat cnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn naggttttta gaatcgacac tttatccata
     1621 tgaaccgata gcaattcttc acgaaagtaa agataaaaaa tggctttttg taaaaatgta
     1681 taattatgaa ggatgggtac aaaaagaaga tgttgcttat acttcattta acatacttaa
     1741 cttacttata aacaatcata attttatagt cataacagaa cctaaagcat atacaaaata
     1801 ttcaaataga gaacttgaca tgggatgtac tttcccatta ttgaaaaaga acaatgagta
     1861 ttttacagtg ctatgccctc aaaaaagagc tgataattct ctttacttta cttttggata
     1921 tattccaata gaacatggga gtattaatta tctgccatat acacagaaaa atacattatc
     1981 tcaagcatat aaatttattg atgagccata tggctggggt ggcatgaatg gatatcgcga
     2041 ctgctctgcg cttgtcatgg atatttacag atcaatgggc ataaagctaa aacgaaatac
     2101 tgaccagcaa gaaatcatgt cacctataaa tatttcagta tccaaaatga gtgaagctga
     2161 taaaataaat cagctaaaga ctcttccagc tggttcttta atatttatga acggacatgt
     2221 catgttatac attggagcat atgaaaacac tcattacatc cttcatgata ctcctggctt
     2281 ctacaacgat tatggatata accctgtaga cagtgtaact gttacaaaag tggatataag
     2341 aaactctcaa ggctatacat atataagcca gtttacaaca tttttatata tatcagaaaa
     2401 agacacagaa tagagatttt cagttcacct aaaaattatg gttataagat aaagtatgcc
     2461 taaaactaaa ggttgatgaa ttatataaat ccacaaagag actcttccag ctttggctaa
     2521 aaataaacat ctttttttag aaaaaaatgt ttttattggt tctctttcaa catagtcaat
     2581 aagatagaat cctataacga aaaaacatat ccagggcaat agtggataat aatcagaaga
     2641 tataattata tctttaatct tgagccctaa aagatgtgac agtccaccac tcatgatttc
     2701 atatggtata tcgaacactt ttaatccaaa aaaactaatt tctctttcat atatattcca
     2761 tgttaaaact gctatgagag cattaatgga aatagcgatt gttgccggta ttttttttaa
     2821 aataggacta agtaactcta ttaagataat agcaaaagca agatgatgaa gaatacccgc
     2881 atatacaaca aactgaggta caacaacatc agttacaaga gtcaggccta ctgctacggc
     2941 cataagtttg agacctcttg atatattatc tttagagtat tttgacacca tacctgaaat
     3001 aagtatgaat agtattgctc caatatcaga aattgtatta actaaagctg attctaccca
     3061 tggcatatca aatccaaaga tatgatacat atcaaaaagc gtatgagaca ctacgactcc
     3121 taatattaaa aagcctcgaa tctcatctag taaatataat ctctgtcttt cacctttttt
     3181 catatatgaa taataatata tttttagaat aaaaaaaaga ccggttgaaa aaccggtcat
     3241 taattttttc aataattttg ttatttgctt tcaatttcac cagtaggttc tgaatcagtt
     3301 gaagaatcat ctactgtttc cacatttcta actgcccatt ttttcatgct cattttagtt
     3361 ctctcaattg atgtctcaat tactatctca tcgtctttaa cattaactat attaccgact
     3421 ataccgccga tagtgcatat gatatcaccg gttttcagtt cagaaagcat ttgttttagt
     3481 tttttatcct ttttcctttg tggtataagg attacagcat acattacaac aaaaacaacc
     3541 actataagaa ttaatgatgt ccagttagtg ccgcctgtct ctgttgctgt ttcagctaat
     3601 attaggttaa acatttatat atcctccgat ttttatttag aatatccata cttttcatag
     3661 aattcatctc tgaaatctaa aagtctgtcc tcctttatag attgccttat tttagccatt
     3721 aagtcaagta gaaatttcaa attatgcaat gttgtaagat ttagtgctaa aatctcatca
     3781 ttttttaata agtgcctgat ataagctttt gtaaagtttt tacatgcata gcaatcacat
     3841 tccggatcca tcggagtaaa atcttctgaa tacttagcat ctcttacaat tacccttcca
     3901 tggcttgtca ttacagtacc atttcttgct attctagtag gcaatacaca atcaaacata
     3961 tcaatccctc ttatggaacc ctctataagg taatcaggag ttccaactcc catcagatat
     4021 cttggtttgt caaaaggcat aataggtgta acatattcta gcatttcata cataagattg
     4081 gctggttctc ctacacttaa ccctccaatt gcatatccag gaagatcaaa tgatgttatc
     4141 tgcttagcgc tcttaatacg aagatcttta tacatacctc cctgtatgat accgaaaaga
     4201 gcttggtcat taggcctttt atgactttca atgcaccttt ccaaccatct tgtagtacgt
     4261 agcaatgaat catctatata gtttctttca gcaggatatg gagcacattc atcaaaagcc
     4321 attattatat cagcacctag attattttct atagacatta ctttttcagg agaaaacata
     4381 tgagaagacc catctatatg acttctaaat gcaactcctt cttcagtaat ttttcttaag
     4441 gaatttaagc taaagacttg aaatccccca ctgtctgtta aaatagaacg atcccaattc
     4501 ataaatttat gaagtccccc tgctttagca actagctcgt cagatggcct aagatacaga
     4561 tgatatgtat ttgataggat tatattagct cctatttctt ttaagtctct aggagtcatc
     4621 cctttaactg tagctgcagt tccaaccggc ataaaaaccg gtgtttcaaa agagccatga
     4681 ggagtatgaa caattcctaa cctcgctcct gactgcttgc atgtgtgtat atgttgatat
     4741 gtaactgggt atttattcat tatataatcc ttttctttta gaaatattag cagattaaga
     4801 aggttttgta aaatattcca ttaattatct ataaacatag catcaccaaa tgaaaagaaa
     4861 cgatatcttt tctctatggc ttgtttgtat gcatctaata cactatcttt tcctgctaaa
     4921 gcgcttacaa gcattattaa tgttgactcg ggaagatgaa agttagttat taagttatcc
     4981 actactttaa aagtataacc tggataaatg aatatatccg tttcaccctg tttttcacat
     5041 agcatgccgt ttgaatcaga tgctccttca agaactctta aacttgtagt accacatgca
     5101 ataatccttc ttccttcttt ttttgctgca tttataaaat ctgctgattc tttagagata
     5161 tagtaatgct ctgaatgcat tttatgatct tctatgttgt ctgatttaac tggtctgaat
     5221 gtacctatac ctacatgcaa agtgactttt acgatatcta cacctttttt ttgtatctgt
     5281 tcaagtaagt ctttagtaaa atgaagacct gccgtcggtg cagcagatga accgctgctt
     5341 ttagcatata ctgtctgata tcggtcatta tcttccagtt cttctgtaat atatggagga
     5401 agtggtacta aaccaatttt atcaagaatt tcctcaaaaa taccttcata ttcgaacctg
     5461 actattctat ttccgaaatc tactacttct aaaacctgtg ctgctaatat tccatctcca
     5521 aaaacaaatc ttttttctgg tatagctctt tttccaggtc tggtaagcac ttcccaatct
     5581 gtttcattaa gtcttctaag aagtaaaaat tcaatctttc ccgtagaatt ctctttatgt
     5641 ccgattagtc ttgcaggaat gactttggtt tcattaagaa ccatacagtc acctttattt
     5701 aagtatccta atatgtctac gaatgatttg tgctccactt tttttgaact cttacttaga
     5761 acaagaagcc ttgatgaatc tcttttatct aaaggatgtt gagctattaa ttcttgtggc
     5821 aggtcataat aaaattcgct ttttttcata ctacctcaat gtaactgatt ccagcataac
     5881 gcctgtatag aaatatttaa tgatctgttc atatgtatat cctgcgtcag ccatattaat
     5941 tgctccccac tgtgccattc cgacaccatg acctgaacct cttccagata ttgatatctt
     6001 atcagtatca tctgtcatta atgaaacagt cactattcct aaattagtct gtacgcctat
     6061 tgtttttgaa cttatcaatg atgtgttata tatacctcta atgctggcat aatccagtgt
     6121 ctggtcagtt gcagcacctg aagcattagc aacctttatt gttgactgac cgcctaaaac
     6181 aaacatctga cttggaagaa aatctggaaa agcatttcta acatttgatt tcaggaatgt
     6241 ggctgagcct gtgcttccat ttacagtcat tgaaagaacc cttcctgtgt gtgaacttga
     6301 atcaatagtt atctttatca catttccaat tctgtatcca agtttataca tatggtcggt
     6361 catttgttga gtagagtatg aataagacca tgtcctattg atatcatatg catctacaac
     6421 agctttaagg tacgatacgg atgtaaccca aacattttca gaattttccg tatacccacc
     6481 tgatgagctg aaatagtatg gtgtaacaac ttttccatta tatgttacaa cggttccata
     6541 tgtctcattt gttgcctgat tagtacgata agtttcggct ttataaccgc catagacttg
     6601 acagcatgtc gttgcacata catcaaatcc atattcctga tgcttattct ggcaaagtgc
     6661 ataagtcctt gcaacaacgg cctgagcttt taaagattca ataggtgaat tacttcctat
     6721 ttctaatgcg actactccat atacataata ctcaagcaac aggttattta atacacacaa
     6781 ttcacttgat ttaaaaatag tgaattccaa attccctcta tatggtttgt tatcaatata
     6841 catatatgat gtgttgcttg tatttatagt ttttgctata acaggtgcag aagggttgtc
     6901 acaggcaaac aaaattctgc tggtatcttt atctcttacc agtattctcg cacttgaagg
     6961 atatgtaatt gttgagtaac tacccggata tcttgcaact acagatgcta tagcattcat
     7021 tgcagaatca gatgatgcgt aactgccaag ccatacctgc cagaatcctg aagctgaata
     7081 tacaggaaaa gtcgaagcac ctgctgcaga caaagccaca gccatatcat aagcttcttt
     7141 gtatgatgta taattatcat ctcttacaat atgatatgaa tattcaccat tatcttttgc
     7201 tacaacaagt gttttgtccg aggaagcaaa atctatctcc tgattgttta tatttatagt
     7261 catatttaat ccaaaaggac tggatattgt atatgattgt ttggctgtat cgccaaagaa
     7321 aagacctacc tgcaaataca aaggagcata aattactgca tttgctacat ttgcatatga
     7381 gaaagacaac agacatatca ttgccagtat tattgagaac ctttgccttt ttttcttatt
     7441 catgagaaat cctttcaatg ttaatcgacc atatctttag tatttatctt aattatatta
     7501 aaattggtga taattacaag caaaacagat aaatcaatta gagtttttga aactttttta
     7561 ctccttttgt tttcacaaaa aaccaaatca tataagttaa tagtgcataa actccagata
     7621 caagtaaaat aaataaatta acatttacat tcacaattgg agatataatc attggcagta
     7681 ctgccaatag catacctgct aagattgtta agcctactga agcagattgt tttaccactt
     7741 ctgtgggtga tttgaattca atcttaggga aaagaagatt cataaataat cctaaaactg
     7801 atgacaagaa tccatatgct ataacaagaa taaaaattac aaaccattca aaaaaattaa
     7861 gacctattcc aatataaact aaaaatgaac ttattattcc tataggcaga gtaacagcta
     7921 atccaagcat catttttccc ataaaaatcg tttcaggttt tacaggtaat gatttaataa
     7981 tccataggtt tttaccttct aaagatatag atgaagcagt tgtagaactc atcataatac
     8041 agaatccaaa aaacaacgct attgcaacca ttttaaaatc agctgggtta aatcctaact
     8101 ctgaaaattc agaaaaaact gaattaaaat caaaatctga caccagtaaa gctatacttg
     8161 caacaaaaag cattaccatg ccaaaaaaag tatttacaat ataaattgta cagtgcgtgt
     8221 atgctcttaa ctcttttttg aaaatagcta ttacaggaga atccatattg tattttatat
     8281 ttttaatatt atgttttgct tttgtttcca tcatagatga gttgatcttt ttaaatgatt
     8341 ttccgaaaag atatataaag agtataaatg gcagaacgaa tattgcaaga aaaagcaata
     8401 atgaaacgat gttaaacctt gctacactgt caacatacat tttaaaaaga aaaaatgatg
     8461 attctattcc agaaatcttt gaaacagcat caactaaagc ttctttgtca gcaagattct
     8521 ggacagaaaa cgatatgccc ataattaaga caagtagtac aaaagaacct attataatta
     8581 ctacattctt acttctcatc tttgccgata ttctagcgac aattagtcca acaaaggaac
     8641 ctatactcat aggaagaaaa ggaataacta aaaagagcag aacatataat acaatagtaa
     8701 ggatattaag ttgcggtgtt tttatgcaat acactaaaaa aatcggaaat cccataagaa
     8761 actgccacaa atatccgttt agcattaatt ttataatctt tgctgctaaa acagtagtat
     8821 ctttgattgg cagagacata agcatatcat aatcactaaa ggaaaataat accgatggca
     8881 ttttgtaaac atccataaaa aagagcataa ataaaggcaa tgcaaatgta aatacaagca
     8941 gaacttccag aagctgcatc tgtagcaaaa catccgatat tattaaagaa gcgccaccta
     9001 tgtataaaaa acagactgca actaccaaaa aagctattgc tgccatacca attgcttttg
     9061 ttctgttttt tgggtccttc aatccattta atttatatga attcactaaa tctgctttta
     9121 ttaacgtaaa aaggctattc atttattgca agctccataa aggtattttc taaagatcta
     9181 tccttaatta cagtttgaat atcacctaaa gcaacaagtt tccccttttt aattattgct
     9241 actttattgc atattttctc ggcaacatca agtacatgag ttgagaagaa aactgcccca
     9301 ccttctttac aaagattctt cattatttct tttactgtaa atgctgcttg aggatctaat
     9361 ccagtaaaag gttcatcaag aattagcagt ttcggactat gaacaagagc tgatattata
     9421 actagttttt gtttcatgcc atgcgaatat gatgaaatta aatctcccag agcttcagta
     9481 aaagtaagtt gttttgcata atgctctgtt aacctttccc gttcgtgctt tgatacttta
     9541 aagatgtcag ctataaagtt tatgtattgt ataccagtaa gtgattcata tacatccgga
     9601 ttatcaggta tgtaagcaat gtcagatttg catcctacag ggtctttctt gacattcttt
     9661 ccatttatga aaatctctcc ttgttcaaat tcaagtatac ctgcaacaca ttttaatgta
     9721 gttgtcttac ctgcaccgtt atgtcctata aatccaaaca tatctcctga cttaatttca
     9781 aaagatatat tatcaacagc ttttatatta ttagcatatg ttttagaaag gttttcaatt
     9841 ttgagcatct tttttacttc tgacctcctt tatcatcata tccttaactt tcataagcct
     9901 tgatgtattt ccgcgttcat tctcatcaag tttcatagtt atatatttaa ttgtttcttc
     9961 aagctgaggt atcatgacat actcaagcgc attaaccctt cgcctggttc tttcaatttc
    10021 tgatgccatt agctgaactg ttttttcttt ttcagcaagc cttataaggt aaggcagaac
    10081 ctcagtaatg ttatataaag cgctgtccag ctctgcagca gtacttaata tgccatatgg
    10141 ataaatgttt gggtcttttt ctgtgtcatc aatatttgtt attactggaa ctttgactcc
    10201 cattatgttt cttgtctgaa catcaagtcg tattctgttc tttggataca taaggctttc
    10261 ttcaattata tcatcagata tggacgctct tgaaagcatg aaatcatttt gggcttctcc
    10321 aagcatacgc tcgcagattt ctcttaattt cttgttttct ttaacaatat tcatgaactg
    10381 tttaactaat tcatctcttt tgtcttttaa gagcttatga cctcttcttg cagtaaccac
    10441 acgctttttt aggttattaa gctccatcct cgtgggattt acattaatcc tggccatcgt
    10501 ctagttcctt aaatttatca tagtattttt caagatttga atctctgact ctcttcattt
    10561 cacttcttgg aagtttgact agcagttccc agccgaggtc taaagattct tctatggttc
    10621 tgttctcata aaagccttga tttatatata tattctcaaa actatcagta aatttcgcat
    10681 aaagcttatc tgtttcagtt aatgcagctt cgcctaatat aatagctagt tctctgactt
    10741 ctcttcctct tgaatatgct gcaaataact gattcattac atcagaatga tcttctctgg
    10801 ttttatcttt accaatacct ttatctttga gacgtgaaag tgatggcata acatcaatag
    10861 gcggcattat tgacttttta ttcaattccc tggatagaat tatctgtcct tctgttatat
    10921 atcctgtgag atcgggtatt ggatgagtct tatcatcttc tggcattgaa agtataggta
    10981 taagagttat gctcccctcg cttcctttac gtcttccagc tctttcatac atagttgcaa
    11041 ggtctgtata gagatatcct ggataacctc ttcttccagg aacctctttt tttgcagcag
    11101 agacttctct taaagcgtct gcataatagg ttatatccgt taatatgacc agaacatgca
    11161 tatcctgctc aaatgctaga tactctgctg ctgtcagcgc cattcttggt gtagatattc
    11221 tttcaatagg agggtcattt gccagattta taaacatgac cgttctgtct atagcacctg
    11281 tctttttgaa atccctaata aaaaagtctg attcctcaaa tgtgataccc atagtggcaa
    11341 atacaacaac gaagttagta tctgtatgta gtactctggc ttgtcttgca atctgtgcag
    11401 ctagctcagg gtgaggaagt ccggaacctg aaaatattgg taacttctga cctcttacta
    11461 atgtatttag agtatcaata gctgatattc cagtctgtat aaactctgaa ggataattcc
    11521 ttgcaacagg attcataggt tctccgttaa catcaactct tttttcaggt atgatatcag
    11581 gacctttatc cataggatta cccatcccgt cgaataccct tcctaacatg tcttttgata
    11641 caggcaaatc aagactctta cctaagaacc ttaccttact gtcagcaagg ttaattcctg
    11701 ctgatgactc aaaaagctgt acaacagcat ttgttccatc aatctcaaga actttgcatt
    11761 ttctaacttc tccattggaa agctctatct cgccaagctc attgtatttt acattttcaa
    11821 catcctttac tatcatcaaa ggaccgtata cttcatttat agtcctatac tcttttatca
    11881 ttatcatgcc tccacttctg aaagattctt taactgacgt tccatttcat catctatctt
    11941 acgaaaatgc tcatttattt ctgattcttc aattaatctg gaacgagaaa ttctttctct
    12001 tacaggaagt tcaaataatt cctcgatatc gatttccttt tttataactt ctttggattt
    12061 atagtaaaaa tccattatag tctttaataa tttatatctt ttattcaaac tgcagtatgt
    12121 atcatttgga tgcatggcat cttgatatag aaaatcttct cttaatgacc ttgcagcttc
    12181 tagtacaagt ctctctactt ttgaaagagc atcgatacca actagtctta cgatttcctg
    12241 aaggttagct tcctcctgaa gaagtctcat agcgtcctga gttaactttg aatatccttt
    12301 agctatattt tcatctgacc ataattctaa tttggatgaa tacagagaat aacttgtaag
    12361 ccagttaatt gctgggaagt gcctagcata tgctaaagat gagtctaaac tccaaaaaac
    12421 ttttacaatt ctcatagtag cctgggtaac aggctctgac aagtcaccac ctggtggtga
    12481 aactgcacca atggctgtga ttgagccatt tctgtcttct gctcctaatg tcataacctt
    12541 tccagctctt tcataaaact gcgctaatct tgaacccagg tatgcaggat aaccttcttc
    12601 tccgggcatc tcctcaagcc ttgctgacat ttctctcaat gcttcagccc atcttgaagt
    12661 tgaatctgcc attattgcga catcataccc catatctctg taatattctg caatagtgat
    12721 acctgtataa attgaagctt ctcttgctgc aacaggcata tctgatgtat ttgcaataag
    12781 aactgttctt ttcatcaaag gttcattgct tcttggatcc attaactgag gaaactccat
    12841 aagaacatca gtcatctcat tacctctttc accacagcca atatatacga ctatatccgc
    12901 atctgaccac tttgcaagct gatgttgaac aactgtcttt ccactaccga aaggtcctgg
    12961 aactgcagca atacctcctt ttgttattgg gaagaatgta tctatagatc tctgaccagt
    13021 aaccaatggt tcttcaggtg cgtatttcat tttgaaagga cgctgtcgtc tgacaggcca
    13081 tttgctcagc atacaaacat caatgatttc atttttatca ttttcaattt tagctatcgt
    13141 ctctgttata ttaaaacttc cttcatatat atcagttatt ttcccattca cattatgcgg
    13201 taccattacc ctgtgttcaa ttaaaactgt ttcctgtatc aaacctagaa tatcaccagg
    13261 ttgcacaaca tcaccgactt tttttacagg tttgaaatcc catttttttt ctctgtcaag
    13321 gcttttaaca tttatacctc ttttaatagt atttccagaa attttccgca tgtcttccaa
    13381 aggtctttgt attccgtcaa atatcccacc aataattccg ggtgcaagct caacagaaag
    13441 tggcatacca gttgtttcca ctgattcgcc tgggcctaaa cctgaagtct cttcataaac
    13501 ctggatactc gccttgtcat ctctcatttc tattatttca ccgataaggc cgtattcgct
    13561 gaccttaaca acgtcaaaca tgtttgcatg tttcatatca ccagctacaa tgagcggtcc
    13621 cgatactttt ataatagtcc ctttgttcat atgtgactct ccattcttaa tcatttatca
    13681 gaatatccac tcctatagca cgttcggtgc atctgcggac attcatcata cctatacccg
    13741 tagtaccatt cattcccggt atagcaataa atgcaggtaa tactttatcc gtatacttat
    13801 ctatgacttc agcattcttt tcatatattt cttcagttac gaaaattact gcatattcct
    13861 gcttatcaag gcttcttaca atatctctaa attcggcctc ttcaacatca tatgctgtaa
    13921 atcctacagc tttgtatgct aatatgtcat cgtgacggcc aataactgca gctttataca
    13981 taacacatcc tcagcctttc tttagtttta tccttactta tcctgttagc ttttcccatc
    14041 atcactattc ttgcattctt gatttcagtt tctttaccta acataaaacc aataacagga
    14101 gctatcccaa taggatcata tctattatct ttcaaaaaag aagtcatata gtcatcacag
    14161 tttttttcca catctcctct gtcaaaaaga gaggcatatc ttgtgtcttt tagaaacttt
    14221 ttaaactcat cttcatcagc ttcatataac cttttaaaat cattaagagt tatactgcca
    14281 tgacttatta atgtccggct taaaaaagat tctttctgaa aaactttctt tatacgcata
    14341 aatgccttta agtttataag gtcggtttga attacagtaa actctttaag aaaagcatca
    14401 tgtgttttat tgattgattc attaaaatat gtatagtaag cctgatccat tattaaatct
    14461 atcagctgag gatcatttga tttagaaaaa tcctcaatgc tttctattat cccatcagct
    14521 attactttag gaagctgtga gaaatctctt tcctgaatga atttcttgag tgtttcatat
    14581 ttaaatactc caacctctga tgcaatttca tcaaaaggtt tcttaagaaa ttccgattta
    14641 agtaatactt ttatgttatg agtatcatat ttaaagaaaa atgcattaaa tatattaagg
    14701 tcaggaataa tgcttcttat atatgaatat aatgaagcat gctcattatc aagcattgcc
    14761 atataatcat catcttcttt atatctgcat tcaatcagta tcttctgcgc ttcagaaata
    14821 tcttgtgcgt caactagtct aagcagcttt acttcatcca tcatgaaatt ctcagctact
    14881 cttactcttc ccactgaata tgcatacttg gtagaatcaa tcactatttg gcattccttt
    14941 caaaaagtat cttcattata tcggtttcca gttcctgttt tttgtatttc aaaattgatg
    15001 aaaaaacaga atttgaatag atattctcat ttctgacaat gacgccttta tcatcttgaa
    15061 taaaatgact gcttaaactt actctaaaat ttgaatcaat gtccttttca agtaaaccta
    15121 taagcttttg gccaatggct ttcgcatcat cttcatttaa cataatttca tttttaccct
    15181 tttcaagaga gccaattatc aaattcttat acattttaag aagatcatct tctggcatat
    15241 tcatcaattc cacatgagca gtctcaaaaa cttttgaaat gaccttctgc ttaactgata
    15301 aaagttgttt tttcccttca aggtttctat ttgctacagc tcttttgata atattttcag
    15361 cttctttttc agcaagttca atttgttcct gttttttttc gtcaagctgc tctttaacag
    15421 cttttttcat aacagaaatt ttttgctttg cttcatcaag cctcttgtct gcctgttctt
    15481 tagcatcatt tataattttg tcatatattc tatctattcc tgacatctta tacattacct
    15541 atcataagta ttgaaactaa aagtccgaag atagcaaaca attcaaccat aagggtaaga
    15601 actactccct taaccatatc attacttctt tttgctacta ttcctatact tgcagcagct
    15661 acttttccct ggaatattcc ggaaatcaat ccgactacac ctgctggaag tgcagcagcc
    15721 ataagactca taccatcagc aaggttcaat cccccaagct taccgataat cataaatgcg
    15781 acaacgaagc cataaattcc ttgcgtactt ggtaatgcct caagaatcat tacaggtacg
    15841 aatttgctgg aatcttctgc aactactccg ctacctacct gacctgctgt ggatacacct
    15901 tttgctgacc ctgcagctgc aagaagaaat cctactgcag cgcctagaat cgctaataca
    15961 tcacctaaat tgttgtccag tacatacttt aacataaatt gttcctccaa ttattattcc
    16021 ttaatcttaa tatatcttgt atcctctaaa agtggcgaga aagctactcc gcctccttca
    16081 aagaatttat taaaaaattc tatatattgc aaacggctcg agtgtacata tgcgcctaaa
    16141 gcatttattg caaagttaaa actgtgtcct ataacaaaaa ttaacccaaa ggataaaaca
    16201 ccaacaatgg taaatcctcc tgaagaacca agctgattga caatagtagc tatgacacct
    16261 gtcgccaatc ccatagcaag cagtcttgaa taagataaaa catcacccat aaaggatatt
    16321 aaatcataaa atgatgctat tcctccaaaa agttttccga atattccttt tttatgccta
    16381 ccctgagtta aaagaattac aattgctgag taaggcagta aagatgtgcc tataggcgtc
    16441 aaagtcgtat aaatattctc tgggacgctg ggaatatatg atgaaactgc aaaaatcgcg
    16501 gtagtaaaga aaaaatacca aggaattaca tctaaaaccg catcaagata tttctttctt
    16561 tttatattat tgtatccttt tataccatat ccgacataaa tatgcagtac acctagtata
    16621 agagaccaca taagtaattt attaggatct tttataggat caaaccacaa tgcaatatca
    16681 attgaagtgt cagaaaacaa tgtaggtata agattgccga accatgaacc atatagagct
    16741 cctacaatca ttgttgctac acctgaaatt agcatcaact taatgaatcg gctggttgaa
    16801 aactccagtt tgaattttaa aagtataata gaacatgcga ttaccataac aagtccgtaa
    16861 cctgcatcag aaagcatcat tccgaagaat aaagcaaaga aaattgatgt gattggatta
    16921 ggatcagctt ctctgcagtt tggcagactg aacatctctg ttactcctga aacagcctcg
    16981 cctaagaaac cattcttaag caggactgga aattcttcat catcatatgg ctctctcaac
    17041 tcaacattac ataaaaaccg tttatctagt tcttctgcag ttttatctga tacttctttt
    17101 ggaagccacc catcaattat aaagctatac ttacctgata atattttttc atcagtgaca
    17161 agcaatcctt ttcttattgc aaaacaatca tatatagcct ttaaatcatc aatttttaaa
    17221 gcatattcat ctaattgctt tttcgcggtc gatatctgct ctttgtaatt ttctatttct
    17281 ttctttttct cggctaatat atctgaagat tttccagtaa aatcatattc taagatatta
    17341 aaacccagac tttttagata atgaattggt ctttctgtat cactttgatg aagatataca
    17401 acaagatatg aattgtttct gtctttgctt atctgttcaa tctgacacaa aaattcctgt
    17461 tcatctatgt gctgtaaata tacctcaggt tttacaaatt cagggattgc accaaatact
    17521 atcttaactt tatcagtttt agataaatta acaggaatat ccatttttat ccatggcaat
    17581 aaagactgac actcattttc caaactgtta atttcgcttt taatatcaat aattgatgat
    17641 tcggctttta taacgtcttc agcaaatttg atatgcttgc tttcattatt caaaatatcc
    17701 tgatagtatt gttgatctat ttcctgtcta accttaaata gaggtttttt tgttttatca
    17761 tatagttcaa gtatttcgat agctttagat atcatagaca tatcactatt tatacctgat
    17821 tcaatatttc tatcatatcc ttttgtaaaa acatcctcat attcagtttc tataggcata
    17881 tcctgaatat ctactacacc acgcttcata agaaaagaaa gtatatcttt cttatcctta
    17941 aataagcctg ttatggatat tttttgcata tcaacgactg ccatctgtat ttgttacctt
    18001 ttcttttata tattttactg cttcttttat atatggttca gctgattttt ctagtttttc
    18061 gcactgattt gcataatcta agtctgcctt agatattata ctctttgctt tttcttcagc
    18121 ttgaataatg atatttttgt attgattatc aatatcctct aaaagttttt tttcatattc
    18181 tttgttttgt ttttctgcat cagaaatcat atactctgct tcatgcatag catctctcaa
    18241 gatatcgcta gcttgttcct cagcctgttt tattctatct aaatatgttt tttccatcct
    18301 tcatttccat tctgttatta tatatatcgg cagttttgcc tttttcttaa tcaaaggtca
    18361 ttataccata atcaataagg gtaataaata taaacctttt aaattaaaga aaataaaaaa
    18421 accgaactcg tataagtccg gattttggca gggaagacag gatttgaacc cgcaacaaac
    18481 ggttttggag accgctgctc taccatttga actacttccc tatgtagcaa tggaaattat
    18541 atagattaaa ctttgacatg tcaacagaaa attaggtatt ggctgaattt atacaacctt
    18601 tcttctatta gttgttcccc ttaatggtgc aagaagctct aataagacat gtatactttc
    18661 agaagtcata tctgctccac tgttttttaa aacttcactt tgtaatgaac tggtaattcc
    18721 caagcattgc atacctgctg attttgctgc ttttataccg cttattgtat cttcacaaac
    18781 caagcagtat gcaggattga gcttcattct ttcagctgct aataagaata tatctggctc
    18841 aggtttattt cttgttacat catttcccgt tacaaatgca ttgaaatcct cttcttccat
    18901 ctctattgct ttgatatttg aagttacctt catcaaatct gctcctgaag caagtgatat
    18961 tctgtatcct ctttttctga gttcattcaa tgtttctttg atatgaggaa ataattctgc
    19021 ttctgtgcaa actaattcat catatatttc atatgctctg tatttcatag catattgata
    19081 ttcaacatta tactttcttg ccacatttcc taaaaaactg ttttcccctt gtcccatata
    19141 cggtatgaaa tcctgaggtt gtgcttctac cccgaattct cttagagcta aaatagcaga
    19201 tttgctcatt aattgttctg aattgataag cactccatcc atatcgaata tgatacccct
    19261 aattactttt tcgtttaaac tcataaccct ccttatattt acacaaacag tatacaagct
    19321 aaaagaacag ccttttcatc actttcattt attacaccat gacctgttcc ttctttgtta
    19381 tatatgcaat caccttttga aataatatat tttttttcat tgttctgatt atatgtcaga
    19441 cttccttcta tcacatagaa aatttctgct tctttctcgt gtacatgaaa accaatggag
    19501 catccctttt caatagttat ggtggaaaac aagcggcctt tgttattcag cattttttca
    19561 ggcagcagtt cattgaacat gatggtccct tgtccgcccc tcatgttttc tttatatgac
    19621 accttttgtt cttctttttt aaatagcata tatagacgtt cctctgacat acggcatttt
    19681 cttctattta ttatatattt ctttatacca ttaatgcaac tactttttaa atataaatat
    19741 gattgatatt aattccaagc tttgatacca ttaaagaaag ttctaatgga ggcagaaaaa
    19801 atgagtaaat atgtatttgc agattggtct gtagttgaac ctggttatgg caccgtatgg
    19861 ccctattcaa agtctatagt tatatctcct tacggtatta gaatagagcc tcataaacca
    19921 tacattgaaa aagatccagt tattctcccg gataaaccat gggaaagctc atgtgtaaat
    19981 tcatatgcga cattcttaaa agctgatggc aaaatcaaag tatggtatga aactttttct
    20041 gatgcaggta tggatgacat gaaaagtaat cttgcatatg ctgaatctga agatggaata
    20101 catttcacaa aaccgaaatt aaatctaatt ccctttaatg gagattacaa taataatctt
    20161 gtaaaaaatg ttcatggcac ttgcgtcctt tatgataaaa cggcagaaaa aaaagaaaga
    20221 tataaactta tatgggtaag ttatgacaga actaaacctt ttaatctttc atgtacactt
    20281 cacggagcag tatcagaaga cggtattaca tggaatgagc ttaatgaagt attaatgaat
    20341 aatcctggag atactcagaa tatattagaa cacgatgaag atttaaaaaa atacgttata
    20401 tacactagac agattaagca ttaccctatc ccaagaagaa gtgtttcaag gacggttagt
    20461 agcagtttta acacatttcc agaacctgac atgattttga ataatgaccc ttgtgatccc
    20521 cccgattggg attattatac ttcaggatat cataaatggc ctggtgcatc aaatgctcat
    20581 ctgatgctta tggatatata taaacgcaca tctgacacat tcgatgtcca cctgctcaca
    20641 agcagagatg gcatagtctg gtacaggcct atgggattac agccatatat tgataaaaac
    20701 tcatatggat gtaaaaaaga ttatatggtc tcttcaacaa aaggaattat tgataatatg
    20761 gatggcacat ggtcttttta cataaattct attcagaacg gacacaatat tccgcctgaa
    20821 gaaagaataa agcctgcagg aggatacaga cgagccgtca tcagagaaga tggttttatg
    20881 tcccttacag cagacagctt aggttgtttt tatacaataa aactagatat tactcaaaac
    20941 tttttaatgt taaatgctga aataccatat ggaggatgga ttaaagtctc catagttgat
    21001 aaagattcat gtaaaccaat tgatggattt gatgaggaca gctgcgatcc aattgatagc
    21061 aaaaaaacat ggaacagtat ttcatggaaa aatatttcag atctttcttc cttaaaaaat
    21121 ggaacataca ggcttaaatt tacattattt aaatcaaaaa tttatgcttt ttcgatatag
    21181 cataggtatt ctacattacc gcttttaccc gctgtatcgc actgcacagt tttgataact
    21241 gtaaatccta tttcttctag atggttgatt atattttcaa cacattgcag tctgactgct
    21301 ttgtctttga caacaccatt cttgccaata ttagttcttc ctgcttcaaa ctgcggtttt
    21361 attaaaaaaa ctgctttagc tccatcattc attagattgt atatactaag cgctgttttt
    21421 tctaaagaaa taaatgatac atcacatgtt acaaaatcta tggattctat aaacactgtt
    21481 ttatcaatat ttcttatgtc agtattttca atacttatta ctctttcatt atttcttaat
    21541 gtatcgtcca actgattgct gccaacatct acagcataaa ccttttttgc tttcttttcc
    21601 aataatacct gtgtgaatcc tcctgttgat gaacccacat caacggctac tagtccgtta
    21661 acattaatct taaaatctga aaaagcctgc agaagcttat atgctcctct gcttacatat
    21721 ttttcatcat tattaatttc aatcttatct ttatcgtaat ctactaaaaa agatggtttt
    21781 gtaattatat taccattaac ttttacatat ccgttcttaa tatgctgttt tgcacttgtt
    21841 ctgctttttg taagtgattt atctgtgaga tataaatcga gcctattcat cttctaacaa
    21901 ctttgaaaga gcatctgatg taagattatt atcagctaat atatcgtcaa cgcaccctct
    21961 catctcacaa tcttccttta tggaaaggtt ttcaaacctg aaactttttt tattattttt
    22021 atacatccat gattcaaacc tttgggctac tgacccatta tatataacat cttctgcaca
    22081 tattacatat ttgtagtcct ttaataggtc atataactgt tgattatcta taggatatag
    22141 ttttcttatt tttacaacag aagcatttga tttttttgct gcttctagag ctaaaggaac
    22201 cattctgcca taacatagga tagctttatc ttttcctttg ttgtaaacag cacattgtaa
    22261 aggtcccaaa tctaaatcac actgtttgct gatatcgctg tttatttcat catatggata
    22321 tcttattgca atgcatccat caagtgaata tatggattca ttcaaatgct tcttcatgtc
    22381 gtccttggat tctggtgcta atatagtcat attaggaaga gtagatagga aagcaatatc
    22441 ataaattcct tgatgtgttt taccatatgc atcagttact cccgctctgt caacacagat
    22501 tacaactttc ttgtttgtca tgcatacatc atgtaatatc tggtcatatg ctctttgtag
    22561 gaacgtggag taaatcacac atatcggctt caaaccattt ttcgacagcc ctgcagaaaa
    22621 agtaactgca tgctgttcgc aaattccgac atcaaaatat ctgtctttat gatttatgct
    22681 gaattcatat aatcctgacc cttcagtcat tgcaggacaa acacatacta tattctcatc
    22741 ttttttcgcc atatcagaaa ttaaacgacc gaaatactcg ttatacatag gatttgcaga
    22801 cagtctcttt cttttggcca gactgttttt aactccatgg aatttaacgg gatctttttc
    22861 agctatatca tagcctttac ctttcgtcgt aataacatgt aaaagtttcg gtcctttgac
    22921 ttttttagtt tcattaatta tttcttcaag agcctgttca tcatgcccat caacaggacc
    22981 tatatacctt atatccaaat cttcaaaaat cattttctgc cctacatatg acttcaatgc
    23041 tcttttagtc ttttgtatta gtttgataag acctttcccg ccgtttggaa gtttctttac
    23101 tagattttta actttggtat ttgtttcaat atatgaagga cgcagtctca gtttagataa
    23161 atatctggac atagcaccaa catttctgct tatggacatt tcattatcat taagaattat
    23221 cagtatatct gagtcaaggt ctgaagcgtt atttaaagcc tcatatacca tacctccagc
    23281 tagagcacca tcaccgataa tagctacaac ctgtccttca atacctttaa gtttcattga
    23341 tttttctata cccaaagcag ctgaaataga tgtacttgca tgtcctgtgt tgaatatatc
    23401 ataaatgctt tcacattcat ctggaaaaca atttaaccct cctttgtttc ccatagtaac
    23461 catacagtct tttcttccag tcagtatttt atgaacataa ctttggtgac ctacatcaaa
    23521 aactatctta tccttttcta aatcaagaca tcggtgtaat gcaactgtta attcaacgat
    23581 tccaagatta cttgcaagat gaccaccgtt catggtaacc acatcaatga gcagttttcg
    23641 tatttgaatg cacaatttat caatattttc tttttcgata tcctttacat caccggatac
    23701 cttaatcctg tctagaactt ccattttgtc atttgtccct atcatctaga taaatcgcca
    23761 tttgacgaag catatcaaat ctttcaccaa aggtatctat ggctctcaat gcattatttg
    23821 tgtgtttgtg taactttttt tcatttgcct taaatccctt tattgagata taagtggatt
    23881 tgtcatttct ttcatcactg ttctttggtt ttcctgtatt ggaattaata tttgtcttgt
    23941 caataacatc atctcttagt tggaatgcta tacctatatt atctgcatag ttttcaagtt
    24001 ttttcaaatc tcttttacct aaacctgcca ttacacttgc tgacattata cttgctttaa
    24061 ttagcgctcc agtctttagt gaatacattt tcaagacttc atcagtattt ttcaaagccg
    24121 acatgtccat tacttgtcca cccaccatac cttcgtgacc gcttgctttt gctatatgca
    24181 aagcagcttt tatatctttt ttagagtctg aatattctaa taaagtttca aaagccagat
    24241 tcagcaatga gtctccagct agtatagcat aagcttcaga aaatttttta tgacttgtga
    24301 gtttccctct tctgtaatcg tcattatcca ttgatggcaa atcatcatga ataagtgaat
    24361 atgtgtgtat catttcaaga gcagatgcga atgcaatatg tttatttgat atctttattc
    24421 ctaaagcctc tgtaatcata aggaataatg caggtcgtat tcttttaccg cctgcattga
    24481 tactatacag gcaagccttt ttcaatatat caggagcact actgttgttt attatttcat
    24541 ttataaaaag attaacgctt tgtccgtatg tgctaattgt tttttccaaa atagtcattt
    24601 atcacatttc cttcaattcc gactttcctg ttacaaccat tttctcacga gctttttcaa
    24661 gtttctcgtt aagttgttta attaattcat gaccttgagt ataggtcttt acagcttctt
    24721 ctagagagag ttctcctttt tccagttgct caactatttt ttccagttcc ttataaagtt
    24781 cattaaaatc cttgtcactc atttttctcc gccttttcta ctatgctgct aatagttcct
    24841 tcaaatattt ttgtgattat ccgatcatct ttgttaacca ttgagctgca cttaattact
    24901 tttccatctt tatcagttgt tatgctatac cctcttgaca ggacattcaa gggatttaaa
    24961 gcttcaattt tctgattgag tatattatat cttgaataaa ttccgtcaat atatgctttt
    25021 gatgccaaga aaaacctatt tgttatatta tccagattaa gtcccagtat attagtatat
    25081 tcttcaggtc ttctcattac cggtctttgt ttaatattta atattttatc atcaagattc
    25141 ctaagtaatt ttgaaaatgc attattaagc gaatctcttt ttaaaaatat atcctttata
    25201 agcatgttct tttcaggcat taccatctga gctgctgctg ttggtgtagc agctcttgcg
    25261 tcagcagtaa aatcagctat tgtaaaatct gtttcatgtc ctatagcaga aataacgggt
    25321 atttcacttg cataaatagc atatgctaat tcctcttcat taaatggcca taggtcttca
    25381 acagacccac cgcctcttgc tattattatt acatcaacat tgttattatc attaaagtat
    25441 cttattccct caataatcgt tttatgagcg tctgtgccct gaactgtagc tggataaagt
    25501 atcattccat aattatcgaa tcttttttgt aaagtattcc ttatatcctg cagcactgct
    25561 cctgttgatg atgttattat gcctattctt tcaggaagat atggtaaagg ttttttgttc
    25621 tcatgactaa aaagaccttc taattcaagt tttttcttta aattttcaaa ctttacaaat
    25681 aagtcacctg ttccgccttc agtcattacc gtaacgatta tctgagtttg tcctgttttt
    25741 tcataaaaag aaagatatcc gtaaacgtat acctttattc cgttttctgg tttaaattta
    25801 agatttcgcg cagatctgtc aaacattaca catcttattg aacagttatc gtctttaata
    25861 gaaaagtata catgacctga ataatgtata gtgcagttag aaatttcacc ttcaactttc
    25921 aaactttcaa gaatatcatc agaagatagc aactggctta catagttatt aagctcagtt
    25981 acagtcagtg tcttttccat tacaaatcct tatcttgcac atttgcaaga acagcattga
    26041 tatattttcc cgcatcatca gaaccatatg cttttgccag ttctactgct tcatttatgg
    26101 caacagattt tggaacatca tcaagattca ttatttcata atatgcaagt ctcataattg
    26161 caagttcaac ttttggaatt ctttcaattc gccagttttt tgcaagatac tgcgatatca
    26221 ctgaatctat ttcctgatga ttctgattga tatagttaac ggcatttact atatattgtc
    26281 tcgcattctc ttcaacttca ttatccataa agtactgttc catctgctcg ttgatgtcat
    26341 cattatttat ttgtatctgg taaacaagct taaaagcatg ttcccttgtc tttcttcttc
    26401 ccatattagt ttttaattct agctgattcc agctattccg tcaacaaaaa ctctgacatg
    26461 atgcaaatcg atacctgtcc cgctcaatat cttttcttta actgcattct gaatttttga
    26521 tgaaatctcc ggtataagca cagtcggcat aaccttgaca tatacatcca tacctatctg
    26581 agtatccttc atatcaactt ttgtacgtat atcagatacc atatcgaatt gagaagcttc
    26641 cattttagcc aaagcttcta atgaataaac agcaattgtt atttgtcctg atggcgtttc
    26701 tatcgaaact gtctctgcag gtttttcctc tttttggatc attgaagata taaacagtat
    26761 ttcaaatcca attactaata gtgcagcaag aaaaagcaat cccctttgag cagaaaaaaa
    26821 tctagcgtca acaaaatctt caaatgcctt ttcaaaatcc aaaggatcta aacaggcata
    26881 tatgacaaaa aaacagattg ccaggaccaa tataatgtta attgctaaaa agaaattttt
    26941 acccttttta aatattttca ttaaggttct ccttaccttt gaatatattg actccctgaa
    27001 catggatgtt tacctcagca actttactgc ccgtcatttt ctctaccatt ttctttactt
    27061 tttcctgtat actccatgca acatctggga ttcttacgcc taattcaaca atgacataaa
    27121 tattaacatg tatgccttct tcatcagtct ctatttttac gccttttgac aaattcttta
    27181 tgccgagcgc ttgagctaat tcgccggtta ttcccccgct catggcataa acaccgtctt
    27241 cttccatagc ggctatgcct gaaaggatgg caataacctc attagtgacc ttaagctgac
    27301 ctaatggtgt ttcaagaccg ctaatattta aagtatcttc tcccatatcg tacctcttat
    27361 gtaattatac atattaaaga ttgcttttac aataatatga aaaactgcta ttcgcagtaa
    27421 ttaggttttg gatatttcgg tgagaagctc tcagtgttta ttgatttagg attgaatatc
    27481 tgcttcagat tactttcaaa tatgctttta agagtaatcg gtgatgatat aatccttgca
    27541 tttattatga tattaaaaga atcatgctcg agtctcattt ggttatttac agatattgaa
    27601 tctgtattag aagtaatgct tgccttgtag aagtcctcat ctgctactgc ataacacttc
    27661 atatgagcta ttgaactttt agattctatc aattcatccc ttaccaagtt cataaactta
    27721 tccataagct tattgatatc ttttacttct tcaaatttca caaatgcatt ggaatttaac
    27781 cagcttaagg aagcttcaac ttctgtatat tttacgtaat ccacttcgat actcttattt
    27841 ataacaaaag aacctgttaa tacaaaattc tttatatcat tgacaaaatc attatcaagt
    27901 gcagatatcg ttttaatctc agtatttgga tacttgcttt ctagaaatac ttttaaatca
    27961 gcttgctcct gctcatttat agtatctttc ttattaatca gaattaaatc tgcttcttcc
    28021 aactgttttg aaaagagata agataccggt gttgtatatt ccggcttttg ttcactcatc
    28081 atcagatgcc ttactctctt aggatcagcc agtaccacaa ggggagaaag tataaaccct
    28141 tcattaaatg aatcaagcat aactgacctg ttgttaacaa aaggtttaaa tattgtagaa
    28201 acaagattaa ttgctgatcc tactggctca agaagaagaa tatctgcagc tttcctatta
    28261 cgaaattcag aaagctttga tgctaagtca tcaaaaaaac agcaaaagca tttaccctta
    28321 acactagtaa catccaaacc gtttgaagat aagaattttg catcaatcag gtcacttccc
    28381 tggtcattgg ttacaatacc tactgacagg ccatctttaa ttaggctttt tgccagattt
    28441 aaaatcaatg ttgtttttcc tgaacctaaa aatccaccta taacagctac ttttgtctta
    28501 tccatttggt ctccttacat caatatatcc tttaagttgt cttttgtgaa gaatgtttcc
    28561 agcggtaatc tgctctttat cgataagtat agcaggaaac aaatcgatct ccagcccgtc
    28621 aactttcctc gcatcttcag gtctttccag tatggttatg acaaaatcca catcaggata
    28681 ctccggcttt attctttcga ataaatttat catcagatca ctgacgataa tacaggactg
    28741 acatggattt ttcatttcaa ccaattgaac tatcatttgc ttttcagttc gatagccttt
    28801 tctatattct ctcttagctg tttcacactt ggtacaatgc ttatgtatct tatttctcca
    28861 tcaattacaa ttgtagggat attagatact ccaagtttca tcatgaaaac aacggattct
    28921 ttatctttta tcttatgttc tacatactca acatcttctt ctaatcctgc acatgctgct
    28981 tttacagctt ccatcatata ctggcaagga gcgcatgaag ccgaatcaag agttacaaca
    29041 tctactataa ctttcttttc tgttttatag tcaggtatta caacttcaat accgtctaat
    29101 gaagactgac ttactattga ttcaggcatc tgtccatggg caacagcgct tattgctaag
    29161 acattatcta ccggtgtagc atatggcata tcgcaacctg gagataaaat atagcctttt
    29221 gtgccaccaa ttgccataca gttcattgca tcattaatat tatcctgtac tgaaccaaat
    29281 agcattgtaa cagtaagttt gatgttacct ccgacagata cattacgttt tttacatata
    29341 tctataacat actcaagcgg aatattctca tcaatagata tattgtcgca ttcacagcgg
    29401 ctcataactt caatgttctt ttttgcatct ccgcaaacaa ataacgaaga cagttttttt
    29461 ctatcactta tatgattaaa tacctgcttg acataaggaa gtacgaattc ttcaaaacta
    29521 tcaggtgata tctggcttgt catagggtca acaaccgcaa taacatcaca tccagcatca
    29581 atatacatat cagcacatgc aatacaaacc ttattacaga attctaaaac ttccataacg
    29641 tgatcgggat catctatcat ttcataaaat atttctgtac ccataagatg aagagctaaa
    29701 gtatagggac cggtaataag cccatacata gcaatgtcat ttccaagatc atcgcataac
    29761 tgttttgtag ctttcataac aagagcgaat cttccatcat ttattgtagg cactttaaga
    29821 tcttccaact taacaccatc ttcaagagga tgtgtcacta cagaaggcgg attatcatca
    29881 gcatatctca acttgcatcc caaagcttca gcttccaatt gaagatcaaa caaaacgggt
    29941 attccgtctg ggtcatatag ttcaaccgct ttcttaactc ctttaactat aagatctcca
    30001 tctttaaaaa attgttctgc agtcacatta tccaaaagtg aagcagcatg acatccaacg
    30061 aaaggaaccc atggggctct ttgtgtctgt ttgttcgtta ctgcgtcaag tacaagctgt
    30121 tttttattca tttttaaata tcctttcact gttatattca gataatatat atatattcct
    30181 accaagtcaa taggaatttc tcactttgtt gtatttttca tttttagaat aagtaaatca
    30241 aatatccagt cgtcttcttt atatgttctt tcattaataa aacccatttt tttatataag
    30301 ttaacagctg cagtatttgt tctatctacc aaaaccgtta tctctttata accgtaatta
    30361 gtaaacgtgt caacaatata acttattaga taagtcgcaa tattcagatt tctgtacgct
    30421 tttttaacat aaagaccaga tatataaatc cttaaaccct ctttggcaac tgttgcatct
    30481 gctgatttta gagtaactgt tacttcagct atagctttat cttcaaacat tactgcgtag
    30541 cattcatcag ttttttcttt aatcttaatt attctttcaa aaaactgtga ctcgtctttt
    30601 atatcatcac gtaaatcttt tatagtgtta aaagaatctt catcaatctt ttctattttt
    30661 aaattattat gctcattcat taaaggataa tcagttcctt atcagagtga ttcagggcag
    30721 tacaccctcc ttgttctaca ataacagtat tttcaattct gactccgcca agatcaggaa
    30781 catatattcc cggttcgaca gttacagcca tattcttctt caaaacagtt ttgcttttaa
    30841 tgctaagtct gggttcttca tgtataagaa gtccaacacc atgaccagta ctgtgtccga
    30901 agtattttcc atatccttct ttttcaatta tatctcttgc aactttatct ccactatatc
    30961 cgcttatacc ttcttttaca taatcctgag cttctttttg agcacgataa actattctgt
    31021 atatcttttc caactcatca gacggttgac ccataaagaa ggttctggtc atatctgagc
    31081 aataaccttt ataattcgct ccaaaatcaa gcgttactgc atcatggatt tgaagttttt
    31141 tattatcagg aacgccatgg ggcatactgg ttttcttacc tgatattgca atggtatcaa
    31201 atgcaggata agaagcacca tttcttttca taacatattc cagttcagca gctatctcta
    31261 tctctgacat gccgattctt actgttttta gaatatggct gaaagcaaga tctgctatag
    31321 atgctgcttt tgacattctt tctagctcaa cttcatcttt aacagaacgt attttaagaa
    31381 ggtcatcata aatgtctaca aaaacatatc catcaagtgc aggttttatg ctgtcaaaat
    31441 aaccatagga catagttttt tcaaaaccta ttctcaatcc tttgctcgtg aaaatattat
    31501 ttaacagttc gacaatactc ttaataccct cttcaattag tataatttca tatcctctag
    31561 aactcaatga agaagctgtt tcgatatatc tgaaatctgt cgcaaaatat gctttatcag
    31621 aagttataac tattacactg tcagtaccac taaattctga tagatacata ttattatccc
    31681 ttgatgtaat gattgcacct tcaaggtttt tttcaactaa tgtttttctt aagctattta
    31741 ttcttgttct aatcattgct gctcctttag aataatctac tgcttatatt tttcatacat
    31801 aattttgctc tttaaagcat ctgtagccat tttatcatca gattcacaaa taaccacagg
    31861 ctgcatatca tatttataca aaacaggtat aaaatcttta aaatatggac catatgtagt
    31921 atcttcaaat gtcagatgct ttttctcccc gccttcagta tattcaattt gagaaaaatg
    31981 aatgtgcatt ttgcgtgttc tttcaattcc cataccatca atcatttgca ccacgactct
    32041 ttcaaagtca tcagatgact tcaggcttcc atgcgttctt gcatttaaat gtccaaaatc
    32101 tactgtagga agtattcttt cttcttttga gcataaatca attatttcct ctaaattacc
    32161 taactgattt atttttccca ttacttcagg gcatattatt acagactcgt catttatctc
    32221 gtctagaaaa cttttcaaaa aaacatatgc tctttttgca gcattttccc gtgtgtcttt
    32281 cattactgct ccaggatgaa cacagattct cgtcgctccc atctgttttg cagcaatgta
    32341 tgactttctc atatgcatta gtgaactttc aactttttgc ggttccttgg ttgccatatt
    32401 aatgtaatag ggagcgtgta aagataaagc tatattatgc tttattgcat attcttttat
    32461 ctgattagca ctatcttctt taatatttac tcccctgcca cactgatact catatgcgtt
    32521 aagtcccata tcttcaagcc actgcattgc ctgatatgtg tttttgtatc cctgatcata
    32581 aaaagaagtc tcatttcccg caggtccgaa tctgatcata tcttttttat cttcctttca
    32641 aattttttca tcacaacttt ttcaaatatc cttttaatcc taatattaaa tgcttcattg
    32701 ttatcaataa tttttgtcat aattgttaag aaacccgcct tattacctcc aagcacatct
    32761 gtgaacatct gatcgccaat cataacacat ttttccttag gaaggttcat tagtaatctt
    32821 gctttgataa atcctttttg tgaaggttta aatgctctgt gtatatattc catttctagc
    32881 atttcaccta tcattgcagt cctcttcttc gatgcatttg aaagtatgca aagagcaaat
    32941 cctttatttt ttaatgaacc aatcaatgat attgccttat gatgcaagtc atcattattc
    33001 atttcaataa gagtattatc tatatcaaat gctaatcccc ttatacccat atcatataac
    33061 ttgtcgtaat cgatatcaaa tatactgtct gaaatatagt ctggcaaata tttttgcata
    33121 aattcctcaa aataattaca atcatttgta attgcattta cattttagta taatatactg
    33181 aaaataaaat taaggcaagg ataacagtat gaatatttca ataaaactga cccagaatcc
    33241 aaaaaagaaa ccagatgaag acaaattgtc ttttggcaaa caatttacag accatatgtt
    33301 tctaatggac tacaaagaag ataaaggctg gcatgatgcc agaatagtcc catatggacc
    33361 tttaaccctt gacccttcaa caaatgttct tcattacggc caaggcgttt ttgaaggtct
    33421 taaggcatac aagactgaag ataatagagt attgttattt cgtccagatg agaacgcaaa
    33481 aaggctcaaa tcatcatgcg aaagattatg cattcctcca attgatgaga tagatgtagt
    33541 tgaaggaata atcaaacttg ttaaaaccga tattgactgg gttccttcat ctaaaggaac
    33601 ctctctatac atacgacctt ttataatatc tacagatcct ttcttaggtg tgcatccttc
    33661 acattcttat cttttcatga ttatactaag tcctgtcgga ccatattacc cctcaggact
    33721 taatccgacc agcatttatg ttgaagattt ctacaaaaga gcatcaaaag gtggtatggg
    33781 ccatgtaaaa actatgggaa attatgctgc aagccttatt gctcagcaaa aagctgaaaa
    33841 attgaactgt attcagactt tgtggttaga tgctcatgaa ggcaaatata ttgaagaagt
    33901 cggtgcaatg aatgtgttct ttgtaatcga caacaaagtt atcactccaa aactgtctgg
    33961 aagcatattg ccaggaatta caaggaaaag cactcttgaa atagcaaaat ctttaggttt
    34021 tgaagttgag gaaaagttaa tatcaattga tgatgttgtt tcagctcttg aaaaaggaaa
    34081 attacaagaa tgttttggaa caggtactgc agcagttata tcacctgtcg gtgaacttat
    34141 ttataaagat actaaatatg taatcaatga taataagatt ggacaaatat ctcaaaaact
    34201 ttatgacaca ttaacaggta tgcaatatgg taaaattgaa gacactttcg gatggactgt
    34261 tcaggtaaag taaaatataa ttcttaaaaa ttaaggaccc atatgggtcc tttttttaat
    34321 cgaatttatt atttatactt atacttcgtt gaattattat cgatttttaa actttactaa
    34381 aactaaggat tattgattat aatagcatgt tgtgcgacat actccataat cttatttagc
    34441 aatatgctgc tttgaagaga ttctttatca tattccttta aaaaatcttc tgtattatca
    34501 taaccataat attcagcata attatcataa ccatcctgta tctctttatt tgtaagattt
    34561 atatcttcta actgtgctat cgcctgagaa atcatctctt gcttcacgcc ttcttgcgcg
    34621 cttagcagat tagtctgatt aaattcttct tctgtctgtc cgagcgaatc aatacagaat
    34681 tcattaaatg aggtgtagcc ataatatgcc atatagtatt cataatactc acgaaccata
    34741 gcaactctct tttcaacagt atttggatat ttttttacct ccgttgaatc aacgataata
    34801 ttccagactg tctggatttt gttatatctt aatatctcct gttgcagaaa ctgattgtgt
    34861 tcccttactg ttttgtaacc tatatttaaa ctacctaaaa attcatcagt atattcagga
    34921 ggaacgcgtc tggttacttt atttacagtt acaataaaga cagcttcttt ccctgctaaa
    34981 tctgaaatat taccattgtt atctttaaaa ttagtcggaa attctgctgt aatttctgtt
    35041 gtctgaccaa tctgtgtacc aataagctcg tcttcaaaat catcaataaa tgtattggag
    35101 cctatctcaa gatcataatc agtgtgtgca ccattttcaa aaggttttcc atcaattgta
    35161 cctgaataat caatatttac aatatccttt tcttgaacaa taccatcttc aaaaaccaca
    35221 acttcagcgt aactgtcaag gaatattgca atttccctgt caaggtcatc ttgagtgact
    35281 tttatttctt catagtctac ttcaacaccc atatactttg aaagtgtaat gtactttgaa
    35341 ttcttatatg gactgctaca cgaaactaat actgataaaa tcagtacaat cattaatata
    35401 aatatgcatt ttttcattaa gtttttagaa tacataatac ctacctgttc taagatttat
    35461 tctttctgtc tctctttctc tgcttcttct tcataaaaac aataatactc tattacatcc
    35521 catatctcct caatacctct tttcgtaata gcagaggtaa gtatgaaagg tgtatcagga
    35581 tctattataa gcattttagc tattttgcgg gcacgagtta tactctgtgc tttagaaagt
    35641 ttatcacatt taccaataac taaaacaaaa ggcatttcag atttatttaa aaaatgtatc
    35701 atttgaatat cgtcgtcagt aggatcatgc cttatatcaa gaatctgaat aattagtcgc
    35761 aaattttttc tttctttaaa ataatcatca gccagattgc tataaccttt agcgatctgt
    35821 tttgatgctt ttgaataacc atatccggga agatcaacaa atctcaactc tccatttata
    35881 tcataaaaat taatcattct ggtcttaccg ggagttgaac cggaatatgc aagattcttc
    35941 ctattacata aggcgtttat aagactggat ttccctacat tggatttgcc tgaaaaaact
    36001 acttcnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    36061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnntcgaa atggtctgga
    36121 ttcaaagcaa aatgctctct tattgatgaa gtcatagcaa gtcttaagtc tgaatcgata
    36181 tttatcttac aaacagccat tgaagctgcc tttctaagca tttcctcagg aactccctgt
    36241 gcaccaggca ttttaccgcc atatttgttt atcttctcta caaactcagg tattacagat
    36301 gatgctccgt gaagcactat cggaaaacca ggcaatcttc tttgaacttc ttcaagaata
    36361 tcaaatctca atttagcctc accctgaaat ttatatgctc catgacttgt tccaatagca
    36421 atcgcaagtg agtcaactcc ggtctttgat acgaattcct caacttcatc agggtctgtg
    36481 tatgaggaat cctctttact taccttgata tcatcttcaa cacctgctaa tcttccaagt
    36541 tctgcttcta ctacaactcc tctttcatga gcatattcaa caactctttt tgtttccttt
    36601 atgttgtctt caaatgtatg gtgtgatccg tcaaacatga cagatgtgaa tccgccatct
    36661 atacaagatt tacaaatttc aaaactgtca ccatggtcaa gatgtacaca aattggcaat
    36721 cctgtatctt caatcgcagc ttctattagc ttcataagat aaacatgttt tgcatacttt
    36781 cttgctccag cagaaacctg aagtatcaga ggagcctgag cctcttttgc tgcttcggtt
    36841 ataccttgga ttatttccat attgttaaca ttgaaagcac caatcgcgta tccgccttta
    36901 tatgcctttt cgaacatttt ttttgatgtt ataagtgcca tttttcttct cctttatttt
    36961 tttactaata ctttttttaa tttagcgaat tttggcagac cttcttcaaa aggcagtcct
    37021 gaatctccct gtattaaagg aagtgcataa tcaataaact cctgctttaa aaagttttta
    37081 tcttcattaa gccattcaag aggaactgtt ttctcagtat ttgcagcatc agaaagtgca
    37141 acaagagtgg tcttgcattc gtacttttta ctattaattt ttctttcaaa acctatcata
    37201 taatcagtat tgccattaac tgcagctttt acagcctctt gacctgcaag aaatgcctca
    37261 tcaatatcag ttcttgaacc tatgtgtgct gcacaacgct gcataaggct gaactctatt
    37321 gatcttactt tacatttaaa ttcattttta actatatcac ctaatacaga agctgctcca
    37381 cctaactgag catgtccgaa tgcgtctttt gctaaagact ggcccatctc agctatatac
    37441 ctgccctctg tgtctttaca accttcagaa aaagcgacta ttactttgtg atcaggtttg
    37501 ctgtcataaa ccttttttac atccttcaaa aatttatcta tagtaaatgg tacttcagga
    37561 agatatatta aatcaggtcc tgctcctttg tatgctgcga gcatgcttgc tgcagttaac
    37621 cagcctgcat ttctacccat tatttcaata attgtaatca ttggagtgtc atatacatta
    37681 gcatcatgat aaacttccat tattgtagtt gcaacatatt ttgctgctga accgtatcct
    37741 ggacaatgat ctgtaacaga aaggtcatta tcaatagtct ttggaactcc aattacttta
    37801 cattcataac cagatttagc aagaaacttg cttatcttgt tacatgtatc cattgaatca
    37861 tttccgccat tatagaagaa atatctgatg ttatactttt tgaaaacttc aagtaatctt
    37921 aaataatcag tttcatcaac ttcagcattc ttaagcttat atctgactga accaagagct
    37981 gatgaaggag ttgtttttaa gagatctaat tgttcctgag tttcttcgga catatcataa
    38041 aattcttcgt tgagaatacc tctgatacca tgtgctgcac cgtaaactgc ggttatacat
    38101 tcttctttta acgcctgttg aaatacacct gatgcgctag aattaattac tgctgtgggc
    38161 ccgcctgact gaccaaatat tgcggctcct tttaacaatg tcatatattt acctccatga
    38221 ccaaaatatc ctagttataa tatcataatt gagtctgtat tacaataata ataagtgttt
    38281 aagcactaag tgtaattatc ctaaaaactg catatccgac cgctatgact aataatccta
    38341 ttgcaattag ttctcttatt ttacctgttt tagctagatc ctttttgaat atcaatatcc
    38401 ctaatgatat tagtataccc aaaactgcaa cagctaaaag cgcataattt ctatataact
    38461 gattatcaga aataaaacgc agaaagataa ataaaaatat caaaacccag tcaataacat
    38521 ttcttttgtt tgtctgattg tttttcatga aaaaagcctt tctttgtatt tgtccagctg
    38581 tagaagtaac aacatgccta aacccagaca tgatactgtc tgcccggctg ctactgatgc
    38641 aacagccagc cagtaatcca tcccatagta tatgttaagt atataaccta ctacaaaagc
    38701 atttatgaca attggcggaa aaggagccag ccatttgctt ttcattctat atgtcaaaaa
    38761 agctgcaagc aaagtcgcta aagaaccaag tacgatatcc agtattcctg cgccgcctat
    38821 aaggttggcc aaaaaacaac caacaaaaag accataaatt gcagaaggtg tgaaatacgg
    38881 aagcacagtt aatgcttctg ctactcttat ctgtacagtt ccgaaactga caggcataaa
    38941 cataaatatc aaagcacagt atatagcagc tattaaggca ggtattatta catatttctt
    39001 aagcattgcc tctccttttt cagtcggagt atatcacagc acaaataaaa tgtgaacttt
    39061 ttattaactt gatacatcta atgaaagtat gaaaaatgtt ttttacaaat tatataattt
    39121 ttttctacat gtgcagtatg tttaggcttt tataaagata ataatgtaaa cattctgtga
    39181 ataatatata ttaataataa atttgtggta aaattactat attaaatcaa aatcaaggag
    39241 aacgtatgaa taacttagta tcattattta gatcaattga catctcaggt aaaatagtaa
    39301 tcgtcgtaat attattagta ttcatagcag catttgtaat aaaccttctt ataaagattc
    39361 agtattacaa attaagcaaa cagattaaca accgtcagaa caggagagca ggcaccttca
    39421 aggatgagat gctcaacgag attgttcagg actataaagt agccggagaa ataaataaca
    39481 ataatgttaa cactcaagct attattgaaa agaatttaac agagcatttg aagttaagct
    39541 cttttggaga gacattcgtt agaaagagca tgtcaatgat gatcactctc ggacttttgg
    39601 gtactttcat cggacttacc atttctgtaa gtgaacttgt aaatgtatta cttcaggata
    39661 taggttctgc aagcctagac tggaatgaga tacttgtcag actagctgca gcagcaaaag
    39721 gaatgggagc tgcttttacc accagtcttg taggactatt cggttctgtt attttaaatt
    39781 tcgcattaat agcaattgac tgtgaagaac agaagagatc attaatgata gatgttgagg
    39841 aatatcttga taacaatgta gctgtactta ttgcaaaaga caaagaaact gaatatacaa
    39901 tgatgaacag aattctcaaa gatactttcg ttgaatttgg ttcaaaaatc gaaatgacat
    39961 tgaaagatac tgtagactca tttgctgata agcttactaa cgttgtaatg gatgtttctg
    40021 tttcatcaca agctcttgat acaacagtag aaagatttga ctcagcaata tcaacattag
    40081 cagttgcaat gaaagatatg tccgacttca atgttaatct taaggaaaat gtagataaaa
    40141 tggatgtttc atttattaag atgagtgaat cattatcaga ttcagcaaac ctgattatga
    40201 aaaattacga tgcaatacgt tcctttgcag aagatgttaa gtcagctgct ggacagatgg
    40261 cggtttctaa taaggaaaca ttggaagagt tggcatcatt agctgaacag gtagatcaga
    40321 ctgtttcagc tctccaacag cttacaacta caatgaaaca gtcaagtgaa gacaatgcag
    40381 aatcaatcaa taacatgaaa gattcatttg aaaaggcaat aatcgctaca tcaatggaag
    40441 tttcatcatt aacagaaaaa attaaatctt ccttcgaaga agctctgaat gaaagcagcc
    40501 agataattgc agaaaaaact gcagctacaa tggaaaagtc catggctaat gtaaattcaa
    40561 tgagtgaatc atttgaaaat aatcagaaaa tactggctca aactattgct tccttaccgg
    40621 agcagactat ggtgtacaat aaatcggttt ctggcaaaat tcaaaagaaa ttggatgata
    40681 ttgaaaaagc catacgtaac gaataacaaa ggattaagag gtgatctaat tgaaacaaag
    40741 agttagttac ttaaaaaaaa cagaagcaga aaactattgg ccttcctttg ccgatgtaat
    40801 gtcgacaatg gttctagtaa tgttattttt ggtgcttatt gttttcataa agaacataat
    40861 aatctccata aaccttgatg aacaggaagc cacaaatatt gctactggcg cacaactaga
    40921 agcaacacga aatgatcttg cagctaaaga attagcactt caggaaaccc agttagatct
    40981 agagacaaaa caaaccatac ttgtaatgct ggaaaacgaa ttggcagcaa aggaaatatc
    41041 actaagcctg cttgaaaatg aacttgtcgc caaacaggca gcattggaac tttctgaaga
    41101 agaagcagca aacctggaaa accttatact tattcttgct gaacaacaag ctgctctaga
    41161 agctcagaaa caagaacttt tatcacttat tgcacttaat acaattgaac tagaatcaat
    41221 gagggaacag ctccagaagg ttgctgtaat gcaggtagat atattcgaaa aggtcaaaaa
    41281 atcaatagat gaagcccttg gagaagggta tgtcactata ggagatgaag gaaaccttca
    41341 aataaatgca agtttactct ttcaggttga cagttccgac atttctcctt ctgcatatcc
    41401 attactggac aagctttctt ctgcatttga ggatattttg aaagattcgg gtcagtatat
    41461 tgaatctata atggtagaag gacatgcaag cagtgatgga agttatgaaa aaaaccaatt
    41521 gctatctact gatagagcta attctgtact tctttatcta tttggcagca atccaaaact
    41581 tgaaacacaa tattcaagtt acttcggagc agttggatac tcagaatcca gacctttagt
    41641 agaaaatgat tatacagaaa ctctaagagc taagaacaga agaatcgaat tttccatagt
    41701 ggttcgtgaa gaaaatgtag gtcagataat acaagaatat ctggattcac ttgatgatgc
    41761 aagttaggag aatacatggc taaaaatgag ttcaaagact taaaattata ttattctaat
    41821 tcgatgatta gccttaagga tggtgattat gatgaggcta taaagggatt caaatatctg
    41881 attaaacatg gtattgaaac acaaaaatca gttttgggtc tgataacagc atattcttgt
    41941 gttactagat ataataatgc attaaagata tatgaagagc ataaggaatt ttttacaggg
    42001 aaaaccccgt ataaaggtat gtttgttgaa ataatgactg cccttctcat aaaggagtct
    42061 acattactta aaaaaaatac cagaggctat ttaacaggta ttataactgc aagaagaatg
    42121 aaggaagttc atgaagctta cttggcaaat ccagacaact tattatgtat catacttata
    42181 tgttactggt atgcagtgat tgcaaaaaga ccaaaagata ctgaacagat gatgatgaaa
    42241 tttgtaaatg acgagcatat tgaggacgag ttcagatgga agctccttga aaaactagca
    42301 attacagaca aacagattat ggaagacata accatcgccg gtaaatttaa aagaatacca
    42361 agatatctag accattcata tgttaatctc cttctatttt ccagtctctc aagcaataat
    42421 cttattatag caagagaaaa tatcgaagtt caaagaatga atggagtaca gttgaacgat
    42481 gacgtaatgt ggaattatct tgatttatgt gtggaaaatg acgatataga tgatttagcg
    42541 gttaattttg caaaaagact tttttctaaa ggatggatgg atccagtaat tgcaaaagta
    42601 ctcagatatg caaaagacaa cctaaatata tataacgtta aaaatgaaat gaaatcttta
    42661 gaactttttg gaatatagtt tacttaaaat tattaaatca cagacagctc agtattgata
    42721 aaaatatgct gagctgtttt tcatatggca tattatcttc actaggaaag tgaataaatg
    42781 aaattttagg ataatccttg ctatctgatt ttgacagaag tctgtagaaa aggtcattgc
    42841 atacataggt accggcacta tatgagtttt ttacaccctc aatttcatat actttatctg
    42901 gatattcaag acttgctgtt ttagcaatat cctcgttggt tacaattaaa gaatcctttt
    42961 taataactcc tgcattatca gcagtatcag aattatcaat attcacagca attttctcaa
    43021 ttgatataaa ttttcttgat gctgccattc cagtagcaat tacaaaatca ggctttattt
    43081 gatcaatcat attatcaagt atcttaaatg cattttcata ctcaaccgga agtataacag
    43141 tgtatatgtc aatattattc aaagacactg gtaaactatt tactatcttt tcagtaatat
    43201 tttcgctaaa accagcaaaa gctatgaatc ctgatactag taccttcatt ttagaggtac
    43261 taatttgaga gcattcttaa agtaaatttt atcctttact tcctgtttaa gatctaatga
    43321 ctcaataagt tcctgatgtc tgttatcaaa atagtctctg gcataaagta ttctgtcttg
    43381 ataattattc atgaaccaat aagcatgttc caaatcccta ttaaatgcat tccatccgct
    43441 tccagctgac atatcacaat acaagttgtc gtatttatcc aatagtcgag aaacctcacc
    43501 accttttatt accgggccct tgggataaga taatttgtca aactgatcgt catttgatat
    43561 atgcgcccag aaacctgggg catgtcctaa aaatgtagtt tcagggcatt tcttaagaat
    43621 tctctctaat acatcaatac caccaccata ccagaaattt ggccaagaat tagaccatgg
    43681 cttattaaat tcataatcaa aatgcattat caccggtaaa ttaagctctc ctgcagctct
    43741 gtaaaatctt atagcatcat aatcatcata catcattcta accttcagtt ccccacatac
    43801 ctgaacatta tgtatgtata ctgcagcttt taaattattt attgcatcag gattacgagg
    43861 atctggacaa tatcctaaaa caaatttttc aggatatgct tttttatatt ccaaagctga
    43921 ttcaaatggt attggacctt ttgtaagata agtagccaat cccccgttat agttc
//
LOCUS       DAOSXM010000010        43196 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2111
            NODE_572_length_43196_cov_10.967757, whole genome shotgun sequence.
ACCESSION   DAOSXM010000010 DAOSXM010000000
VERSION     DAOSXM010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864178
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 43196)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 43196)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:24:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,261
            CDSs (total)                      :: 2,229
            Genes (coding)                    :: 2,218
            CDSs (with protein)               :: 2,218
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 3 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43196
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_572_length_43196_cov_10.967757"
                     /isolate="AMR_MDS_2111"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(96..1073)
                     /gene="tsaD"
                     /locus_tag="PLQ68_02770"
     CDS             complement(96..1073)
                     /gene="tsaD"
                     /locus_tag="PLQ68_02770"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016208804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HXK71604.1"
                     /translation="MIILGIETSCDETAASVVKDGRIVMSNIIASSAQIFEEYGGVVP
                     EIASRKHVEYILMVIDQAIKKAGIKKEELDAIAVTQGPGLVGALLVGITAAKALAAAL
                     DIPLIPVNHLKGHIAANYITHKDLKPPFVCLLVSGGHTNILNVNNYDDIQNIGSTRDD
                     AVGEAYDKVGRVMGLVYPGGPKIDKMAIEGNPEAILFPRTKFKDNPFDFSFSGLKTAV
                     INYIHSKEQKNEEYSKKDVAASFQKAVTDVLVENVFLTKSEKIVVAGGVAANQGLRKA
                     LYERADKEKVQVYFPDIHLCTDNAAMIGSAAYYIKEEDFDINADPSLEM"
     gene            complement(1070..1855)
                     /locus_tag="PLQ68_02775"
     CDS             complement(1070..1855)
                     /locus_tag="PLQ68_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015709832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HXK71605.1"
                     /translation="MSIWEAVLLGIIQGFTEFLPVSSSGHLVLFEKILNISTPTLTFN
                     VAVHFATLISVCAVFWKDILAILKKPIQKLTLWLIIATIPAAIAGVLFNDLFEGFNDS
                     GVTLGIGFLITACFLIISSKLKPGTMEKENMKWYDALFIGIMQMVAIVPGISRSGSTL
                     TGGLFRKVKKEDAVKFAFLLSIPVILGGFLLETYDAVKAGFENIEILPIFFGMIFAGV
                     SGFFAIKIFVKTVLKGKIQYFAYYVAILGIFCILDQFVFNIVL"
     gene            complement(1852..3126)
                     /gene="clpX"
                     /locus_tag="PLQ68_02780"
     CDS             complement(1852..3126)
                     /gene="clpX"
                     /locus_tag="PLQ68_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HXK71606.1"
                     /translation="MSNEIKKIKCSFCGKSEDQVQRLISGPNVYICNECIDLCSDLLD
                     SEFGYDDVEELDKEPMIDEVPRPAEIKRRLDAYLIGQDDAKKAISVAVYNHYKRLSHN
                     VTSMPEDDIEIQKSNILMLGPTGTGKTYMAQTLAKILNVPIAISDATTLTEAGYVGDD
                     VENILLRLIQAADYDVKRAQRGIIYIDEVDKIARRADNPSITRDVSGEGVQQALLKIL
                     EGTVANVPPQGGRKHPTQEYIQIDTTNILFICGGAFQGIDEIIQNRLGKRAIGFGAEI
                     LSSKSIEKDKVLKHIENQDLLKYGLIPEFVGRLPVVVTLEQLTEEILVSILKEPKNAL
                     TKQYKKIMEMDGCELIFEEKALYAIAKKTLERNSGARGLRSILESVLMEVMYNVPSDP
                     TIEKCIVEESTILNCTPPKLIYRGKLLEESAS"
     gene            complement(3140..>3313)
                     /locus_tag="PLQ68_02785"
     CDS             complement(3140..>3313)
                     /locus_tag="PLQ68_02785"
                     /inference="COORDINATES: protein motif:HMM:NF012783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HXK71607.1"
                     /translation="RLHTEWLLKVKENLNRILADKTGQSLKKIQKDVERDFFMSSADA
                     KEYGIIDEVLVKR"
     assembly_gap    3314..3413
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3414..3514)
                     /locus_tag="PLQ68_02790"
     CDS             complement(<3414..3514)
                     /locus_tag="PLQ68_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012994730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HXK71608.1"
                     /translation="MNTIPYVIEQTSRGERSYDIFSRLLKDRIVFLG"
     gene            complement(3535..4971)
                     /gene="tig"
                     /locus_tag="PLQ68_02795"
     CDS             complement(3535..4971)
                     /gene="tig"
                     /locus_tag="PLQ68_02795"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HXK71609.1"
                     /translation="MENKIVKKEGYTVELEIALTKEEFEGYITKAYNKDKSKFTVQGF
                     RKGKAPQYLIERVYGKGVFYDTAIDMSLNTEYPKAIDDLKLEVVDYPNVDLKDMDEES
                     GITFTIKVDVKPDVELGEYKGVEIQKAVVEVTDKDVEDALNSEAEKNSRMISIEDRPV
                     QEQDTVIVDYEGFVDGVAFEGGKDEKHELVIGSKSFIPGFEDQIIGHKINDEFDVNVK
                     FPDEYHSEDLKGKDAVFKVVLHEIKVKELPVIDDEFIKDISEYDTVEQYKEETKKKIL
                     EDRQKKAEDSMRSQALAVACENAKADIPNAMIEQATDRIIDNMKMEMQYSGYTLEQYL
                     EYTGMKLEDVRKQYAEQAKMMVVRDLVIEAIRKSENIEVTDEEIEEELKKEAEMYKME
                     VDKIKSAIGDYKKYYTDRVADEKTIDFIYASAKKVKEVKAKKEAAKKQTEKKDNKEEG
                     EAKKAEPKKKAAPKKSTKKAEEDKTDKE"
     gene            complement(5076..>5805)
                     /locus_tag="PLQ68_02800"
     CDS             complement(5076..>5805)
                     /locus_tag="PLQ68_02800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK71610.1"
                     /translation="NMYGYDDVTYSMTGRMNDDKIEIVDNSEQDRISYFFDGIMNILV
                     NNEDFYTVIMSDLVYDAYTSGYLENIDFNNYANYKLVSDKSDKGLREIVYSFEINDIL
                     TSEFSLWNLSKGETILSTYVLDNDWQIISHDFKLDVKGGDNLTLMTKTFTYGNELINN
                     NILESYIQSQDRISVTIINTVNEEEEYITYSIPKGTYFGYDFSDSVMDLYYDSLCKDL
                     FKYADQIIEQDITLYIGQSQYAGE"
     assembly_gap    5806..5905
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5906..>7158)
                     /locus_tag="PLQ68_02805"
     CDS             complement(<5906..>7158)
                     /locus_tag="PLQ68_02805"
                     /inference="COORDINATES: protein motif:HMM:NF036578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-rhamnosidase C-terminal
                     domain-containing protein"
                     /protein_id="HXK71611.1"
                     /translation="GFRYAQIEGFATKPDTSAATAVFLHTAVKEIGQFSCSDEIINQI
                     QKNTRMATYSNMYGLLTDSPHREKNAWTGDTNVSTEQMLFNYMSGPIWIKWLNDIKDS
                     MKAKGNIPCMVPTAGWGYNWGNGPDYSSVLTWLPDHMNTYYSDKVMICKYYEYMKRNM
                     FYMLSMMQDDVCVNDYGVGDWCAPFEGPAISVNMSSFKAPIDLTDTACMYTMANVLEK
                     FAKMVGIREDAKEFSKIAKRIKAGFRRAFFDVLTGRLKGDGQTCYAAMLYHNLYENEK
                     EYDLIYSHLIRTINEADGHLDYGILGNKYVNNILGKKGRADVIYSMITQDTFPSFANC
                     IKELGATTLYECWNGEGSRNHHMFGDISATFFKYFAGIQPDTEYPGFKRFTLAPGFNT
                     PLEKVDAVYESMLGTIRCSYVKTEKS"
     assembly_gap    7159..7258
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7259..8099)
                     /locus_tag="PLQ68_02810"
     CDS             complement(<7259..8099)
                     /locus_tag="PLQ68_02810"
                     /inference="COORDINATES: protein motif:HMM:NF020120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-rhamnosidase N-terminal
                     domain-containing protein"
                     /protein_id="HXK71612.1"
                     /translation="MSITIKDLTVNYLENPVGIDQVPRFSWKLIQDGRGNRQRSYRIK
                     VMTGGKLVWDSKNIRSDESVLIEYEGKKLMPRTTYRWNVSITDKSGERAQSEDGFFDT
                     GKLEEKWEGRFIQSGFKVPRELMASSPYLRKRFNVNNDVSKAMLYIAGAGFFEPYVNG
                     KKINDHIMDPPYTAYDKTLLYYSFNVTEYLKKGSNMLGVILGNGFFNLPTTDQWSTQA
                     ASWKGSPKLICELHITTKDNKQICIPSDITWESHMSPITFNSLRNGEYYDARLEIPNW
                     CDAD"
     gene            complement(8298..9503)
                     /gene="tuf"
                     /locus_tag="PLQ68_02815"
     CDS             complement(8298..9503)
                     /gene="tuf"
                     /locus_tag="PLQ68_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020457902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HXK71613.1"
                     /translation="MAKAKFERTKPHCNIGTIGHVDHGKTTLTAAITKTLALADKNIV
                     FKAYDQIDAAPEEKARGITIATAHVEYETKNRHYAHVDCPGHADYVKNMITGAAQMDG
                     AILVVSAADGPMPQTREHILLARQVGVPYIVVYMNKTDQVDDPELLELVEMELRELLS
                     EYEFPGDDIPIVMGSALKALESTSTDQTSEEYQSILKLMEAVDSYIPTPERAVDRPFL
                     MPVEDVFSITGRGTVATGRVERGTIKVQDTVEVVGLKETVSSTVVTGVEMFRKILDFA
                     QAGDNVGCLLRGVQRTDIERGQVLAKPGSITPHTEFEGQVYVLTAEEGGRHTPFFTGY
                     RPQFYFRTTDVTGVANLPDGIEMCMPGDHIDMKISLITPIAMEEGLRFAIREGGRTVG
                     AGSVAKIFK"
     gene            complement(9580..11655)
                     /gene="fusA"
                     /locus_tag="PLQ68_02820"
     CDS             complement(9580..11655)
                     /gene="fusA"
                     /locus_tag="PLQ68_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013296974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HXK71614.1"
                     /translation="MPRDYALEFTRNIGIMAHIDAGKTTTTERILYYTGKLHRMGEVH
                     EGAATMDWMEQEQERGITITSAATTAYWKDYRLNIIDTPGHVDFTAEVERSLRVLDGA
                     VSVFCAKGGVEPQSETVWRQANRYKVPRIAYINKMDTTGADFYRCIDMMKKRLNANAI
                     PLQIPIGAESTFTGIIDLVKFKAYYYMDDLGNNIEERDIPENMLELATDYRIRLIESV
                     CDEDENLMEKYLEGETVSDEEIKKAIRIGTIKLTITPVICGSSYKNKGVQKLLDAVTD
                     YLPSPIDVHAIEGVDMNDHSKVITKPADDDDSFSALAFKIMSDPYVGKICFFRVYSGT
                     LNTGSYVLNATKNKKERIGRILMMHSNSRTDIDKVYSGDIACAVGLKFTVTGDTLCDA
                     KDPVILESMQFPEPVISISVEAKDKANQEKMTSALQKLSDEDPTFKYKTDSDTGQMII
                     SGMGELHLDILVDRMRREFQVDATKGRPQVTYKETITKPMRGESKFVRQTGGHGQYGH
                     VVLDIEPAKQGDGFTFTDKTVGGAIPKVYIPAVEKGIKESLGNGIKAGYPIVDVAVTL
                     VDGSYHEVDSSEMAFMIAGSMAFKEACLKADPILLEPVMRVDVFVPENYLGDVLGDIT
                     SKRGKIENVEARGIESIITSVVPLKEMFGYATDLRSNTQGRGTFTMQFDHFEKVPDET
                     AAKILKTNY"
     gene            complement(11671..12141)
                     /gene="rpsG"
                     /locus_tag="PLQ68_02825"
     CDS             complement(11671..12141)
                     /gene="rpsG"
                     /locus_tag="PLQ68_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014312030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HXK71615.1"
                     /translation="MPRKGHVPKREVLADPLYKDKVVTKLINNIMLDGKKGVAQKICY
                     DAFEIVKTKTGRDPLEVFKQAMDNIMPALEVKARRVGGATYQVPMEVRPERRQALALR
                     WLTQFARSRNERTMKERLAAEILDAVNGVGNTFKRKEEMHRMAEANKAFAHYRW"
     gene            complement(12255..12665)
                     /gene="rpsL"
                     /locus_tag="PLQ68_02830"
     CDS             complement(12255..12665)
                     /gene="rpsL"
                     /locus_tag="PLQ68_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HXK71616.1"
                     /translation="MPTFNQLVSNGRKVSNKKSLSPALQKSMNTLKKRTTTVACPQKR
                     GVCTVVKTSTPKKPNSALRKIARVRLTNGYEVTSYIPGIGHNLQEHSVVLIRGGRVKD
                     LPGVRYHIIRGTLDTQGVANRKQGRSKYGAKKGK"
     gene            complement(12698..12931)
                     /locus_tag="PLQ68_02835"
     CDS             complement(12698..12931)
                     /locus_tag="PLQ68_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal L7Ae/L30e/S12e/Gadd45 family protein"
                     /protein_id="HXK71617.1"
                     /translation="MAVHENKVIGLKQTVKAIKQNRAKLVYVAEDANSSLTGPVLEMC
                     KKKEIDVEYVKTSRELGLLNGISVCATVVCILK"
     gene            complement(12940..16644)
                     /gene="rpoC"
                     /locus_tag="PLQ68_02840"
     CDS             complement(12940..16644)
                     /gene="rpoC"
                     /locus_tag="PLQ68_02840"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006862671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HXK71618.1"
                     /translation="MQDYANFDMIKIGLASSELIKEWSRGEVKKPETINYRTLKPEAG
                     GLFCEKIFGPTKDWECHCGKYKRVRHKGIICDRCGVEITKSKVRRERMGHIELAAPVS
                     HIWYFRGIPSRMGLVLDMSPRSLEKVLYFNSYIVLDVNNEDVKVNDKTIEVKDLLTEM
                     QYREMLDKYGRGSFVAKMGAEAIKILLERIDVESESAQLKVDLQSSTGQKRGRIIKRL
                     EAMESFRVSGNKPEWMIIDTIPVIPPDLRPMVQLDGGRFATSDLNDLYRRVINRNNRL
                     KRLLELTAPDIIVRNEKRMLQEAVDALINNGRKGKPVVGPGNRPLKSLSDMLRGKQGR
                     FRQNLLGKRVDYSGRSVIVVGPELKMYQCGLPKEMALELFKPFVMKRLVTDKFAQNIK
                     SAKKMVERIRHEVWDILEDVIKDHPVLLNRAPTLHRLGIQAFEPILVEGRAIKLHPLV
                     CTAYNADFDGDQMAVHVPLSAEAQSEARVLMLSSNNLLAPKDGKPITVPTQDMVLGGY
                     YLTTAKDGEVGEGRVFRDFDEMLLALDEKTITLHSKVKMRTYKEIDGKIQSRIINVTC
                     GKVIFNEAIPQDLGFVDRSNPETMFDLEIDQMVGKKDLSKIIDRCITKHGIAKTAEVL
                     DKIKTIGFKYSTQGAISISVSDMIIPSSKKETLAETEIKVDKVTKDFRRGLITDEERH
                     DQVVKLWRDTTDSVTKMLMDGLDAFNPLNIMSRSGARGDTNQIKQLAGMRGLMFDTSG
                     KTIEIPIKSNFREGLNVLEFFISSNGARKGLADTALRTADSGYLTRRLVDVSQDVIVR
                     DEDCGSEDDILVYDVVDSGEIVVGLRERIYGRYVVKEIVNPNDKNEVIVEAGGYIDAD
                     AANRIIEAGIKQVRVRSVLTCKSHLGVCAKCYGMNLASSKPVNIGEAVGIIAAQSIGE
                     PGTQLTMRTFHTGGIAGDDITQGLPRVEELFEARKPKGLAIIAETEGIVKINETKKRR
                     EVIVTSKDESHTYTIPYGSRLTVEDGDEIGKGDKITEGSENPHDILRIKGVDGVHRYI
                     ACEVQKVYKLQGIDINDKHIEIIVKQMLRKVKVEDCGDTNLIPGSYCDATEFEDQNEI
                     ALENNKQPATGERVLLGISKASLATESFLSAASFQETTRVLTEASIKGKTDNLIGLKE
                     NVIIGKLIPAGTGMNAYQDPYLEMDPTEDEEMISEEAFDEEFDLFDDLEDEVENLDEN
                     DSEEYDNEGLLEESDSDDENEPEEKEEEKE"
     assembly_gap    16788..16887
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<16888..20279)
                     /gene="rpoB"
                     /locus_tag="PLQ68_02845"
     CDS             complement(<16888..20279)
                     /gene="rpoB"
                     /locus_tag="PLQ68_02845"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012268991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HXK71619.1"
                     /translation="MIHPIQYGKRERMSFSKIKEVLEMPNLIEVQKKSYQWFLDEGLR
                     EVFKDVSPVRDHTDNLILEFLDYRIAESPKLDVQECKERDATYQAPLRVKVRLIRKDT
                     GEVKEQEIFMGDFPIMTDTGTFIINGAERVIVSQLVRSPGTYYAEKKDKTGKSLYSST
                     LIPNRGAWIEYETDSNGILYVRIDRTRKLPLTVFLRALGFATDAEISAVFGEDYKLDA
                     TYAKDETKTREGGLFEIYKRLRPGEPPTTESAESLFEGLFFDPRRYDLARFGRYKFNK
                     KLSIAPRLVGNISAETIIDKSTGEILVSEDDKITEEQANAIENSGINVVKVKCDNHVH
                     VVIGNDTVNAAYFVDFDYKALGLTDKVSYKVLKDILDTYDNEKDIKEAFEKRKYELCP
                     KNITKYDIIASISYILGLDYGLYNVDDIDHLGNRRIRSVGELLQNQFRIGFARMERVI
                     REKMTIQDIDIVTPQALINIRPVTAVIKEFFGSSQLAQFMDQPNPLAEITHKRRLSAL
                     GPGGLSRERANFEVRDVHYSHYGRMCPVETPEGPNIGLIASLSTYARINEYGFIETPY
                     RKVNKETHVVTEVIDYLTADEEDNYIVAQANSELTDDGHFAQPRVSCRYRNNKVMRDS
                     NTVDYMDVSPKQLVSVATSMIPFLENDDANRALMGSNMQRQAVPLIKPEAPIIATGME
                     YKVATDSGVCVLAKREGTVEKVSADKVVVVTKDGQKDNYNILKYKRSNQGTCINQKVI
                     VKKGEFVKAGDVIADGPSTDGGEISLGKNILIGFMTWEGYNYEDAILISEKMVMEDVF
                     TSIHIEEYDLEARDTKLGPEDITRDIPNIGDEALKDLDERGIIRVGAEVRSGDILVGK
                     VTPKGETELTPEERLLRAIFGEKSRDVRDTSLRVPHGESGIIVDVKLFTKESGDDLSP
                     GVNQVVRVFIAQKRKISVGDKMAGRHGNKGVISRVLPEADMPFLPDGTPLQIVLNPLG
                     VPSRMNIGQVLEVHLGYAARALGWQIATPVFDGASEMDIIECLKMAGLSEDGKTILYD
                     GRTGQPFDNRVTVGYMYMMKLHHLVDDKIHARSTGPYSLVTQQPLGGKAQFGGQRFGE
                     MEVWALEAYGAAYTLQEILTVKSDDVIGRVKTYEAIVKGESIP"
     gene            complement(20520..20816)
                     /locus_tag="PLQ68_02850"
     CDS             complement(20520..20816)
                     /locus_tag="PLQ68_02850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK71620.1"
                     /translation="MKKTDVDNFLNFEDDIFEELRLDEADMALLEKIDHEIESTLKNI
                     RIYDLQTNKKMADFLSEEINNQGERINKLVDDASPQLKKFLVGRIEKIKGTYLN"
     gene            complement(20803..21483)
                     /locus_tag="PLQ68_02855"
     CDS             complement(20803..21483)
                     /locus_tag="PLQ68_02855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK71621.1"
                     /translation="MVKKTLMIMIAVVLMFSLCSCNNKTDETNSPGPTHVISSDADWP
                     TEYAGSDVLPKIQDGDVNYYKLYDRVLRIYIYNVDENSFLQYIELLKQTGYSDIAVET
                     KEAFSGNLNEYSIVMNRDNDTNRMVLELTTPLSWPKEGYITMLPVPPDVKIIQIIGES
                     NSITVIAEDIGKTNFAAYTADIIAAGFKDILVTSDTVFVAENEEYIATIVRQPEKNTM
                     DIGVYEKN"
     gene            complement(21495..23615)
                     /locus_tag="PLQ68_02860"
     CDS             complement(21495..23615)
                     /locus_tag="PLQ68_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015923819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HXK71622.1"
                     /translation="MEDKIIKASMLSTEVINEAKKGAIAHSILEKHDLSDKKGNLEMR
                     FDAIASHDITYVSILQTAAACNLQRFNIPYVLTNCHNSLCAVGGTINEDDHLFGLSAA
                     KRYGGIYVPAHMAVIHQYMREMMAQCGHMILGSDSHTRYGALGTIAIGEGGGELAKQL
                     LGKTYSIKYPEVCLIYLKGQLKKGVGPHDVAIYMIGKLFKKALVTNKILEFAGPGIKN
                     LSMDDRMGIDVMTTETACLSSIWVTDEMTRKYYEIHGRKDAYKKLQPADKAYYNCALV
                     IDLSEIEPMIALPYHPSNAYTIREFLDNSLDILHSLKLDSLNEFADTKNKSFFSGQGI
                     IAGCAGGTYENIIEASEILEKGDTGSDRFSLSVYPQSQPVAYALNKIGCMNKLMEAGA
                     VIKTAFCGPCFGAGDVPANNTLSVRHTTRNFENREGSIPKNGQSAYVALMDARSIAAT
                     ALNKGYITPGTDIDYEKSIPEYEFNKSIYDRRVYYGYNKPDYSQDLIYGPNIKPWPEI
                     PKCPENLLLKVAAVLKDEVTTTDELIPSGETSSYRSNPMALAEFTLSRKAPDYVMNTK
                     MALKEEEVDGHLIFAKKPGDGSAREQAASCQRVLKGFANIAYEYATKRYRSNLINWGM
                     IPFIIKEELELNTNDYVYIPGIRKAILNGEETIKAYIASDKQTVMTKDSKAKVVILYM
                     EGLSETERDVLADGCMINYYRKSI"
     gene            23723..24349
                     /locus_tag="PLQ68_02865"
     CDS             23723..24349
                     /locus_tag="PLQ68_02865"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HXK71623.1"
                     /translation="MKYKHIIWDFDGTLFDTYGEISLVITEVLKKKNIVEDNNYISKN
                     LHKSLTHTLKLLSKKHLLDFDKLVSEFMFAEEKMNVKKAMPFNGVKKIIESIEGYNFI
                     VTNRGESVFRFLEDKDYLRFFEEILYRDSGLELKPSSATVDYLVSKYNLDKSETLFIG
                     DRDIDIECANNSNIDSCYYDSHNIINTAPCTYYVKDYKTLIELLDISK"
     assembly_gap    24419..24518
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<24519..25434)
                     /locus_tag="PLQ68_02870"
     CDS             complement(<24519..25434)
                     /locus_tag="PLQ68_02870"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HXK71624.1"
                     /translation="MELREDRKLQIHQSFLLIAVVMIIFLAVQVVGMFFLMDNLYLYQ
                     AISEILGLAIPVLAFLLITKRAKDNIHIRRVSIKNTLTISAIIALFIPVSMLLATFNM
                     WITEHTIGLTAGLEDSIMIDNFPDYIKSILVLALVPAVCEEIAFRTAFMSGLERLGKT
                     SCFIFVSLFFSLAHMIPEKLFSTFALSILLCYFLYRTGSVISSFIGHFVNNLIAITIT
                     YVASVLPSTVSSEDVSEFYGLSEYGISFIVWGALTLVVLPLIIILIKKLRKNTEHTKL
                     NISKQAKMDANAMSAFLPALAVYVILLSI"
     gene            complement(25434..26402)
                     /locus_tag="PLQ68_02875"
     CDS             complement(25434..26402)
                     /locus_tag="PLQ68_02875"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HXK71625.1"
                     /translation="MIKITIKNNNDSLSLDKLAFGTGCFSDYNDKESYFVLMDRFFEN
                     FRTFDTARSYNEWLPDGKDISEYMLGQWMKSRNNRDETVIITKGGFPKNLKIKKDVVN
                     RITKEDLQYDLDTSLKTMDIDCVDVFLLHRDDESKPVDDIIPILDNFVKQGKTRFVGV
                     SNWKPERIEKANEFIVKENMSPLVLSQCYFSLAESTPEKFDDSTIVCINKAEYEWYEK
                     SKMPLMAYTSQAKGFFTKIHNGNMKKSDIVRFMSDDNIERARKAKKLADEYSVSPTCI
                     ALAYLTNNKVQTSAIIGPRNLSQLEDSMKVKDVMLSKDQIKWLNGE"
     gene            complement(26399..27580)
                     /locus_tag="PLQ68_02880"
     CDS             complement(26399..27580)
                     /locus_tag="PLQ68_02880"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011722767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HXK71626.1"
                     /translation="MFNKKITENLSKSSFIRAMFEEGNRLKAIYGEKNVYDFSIGNPE
                     MEPPVEVKQSLLDIAMSKEEGMHKYMSNAGYEQTRKAVAKSIKNDYDYDIPYKNIIMS
                     AGAAAGLFVTLKSILNPDDEVIVFKPYFAEYGFYVMHAQGNLKVADCDESFMPDIDSF
                     MRTATSRTKAVIINTPNNPSGVIYPKEVLLKLNEAIRQVEKKYNTDIFVLSDEPYAKI
                     VFDDATVPCVFKCFDKAIIINSYSKSLALPGERIGYIAVDHKIPNAELLLNICIFNTR
                     TLGYVNAPALIQRVIQQSCEAKVDVEDYKRRRDLLYNHMIECGFSIKKPQGAFYLFVK
                     SPVEDEMEFVEKAKGFNILIVPGRGFGMPGYIRMCFCVSYETIKSSLPKITQLAKEYF
                     K"
     gene            complement(27638..29416)
                     /locus_tag="PLQ68_02885"
     CDS             complement(27638..29416)
                     /locus_tag="PLQ68_02885"
                     /inference="COORDINATES: protein motif:HMM:NF017633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT RNA binding domain-containing protein"
                     /protein_id="HXK71627.1"
                     /translation="MPLDGMAVRCLANELNQLCRYSKIQNIFMPRNNEVVLSIYGDKK
                     SHYLTISMEPSSPGIYLRYDKMDNPKTPYSFCMFLRKHIGGGWIERVYTKDYERVILF
                     DINHTDELEETSKKTMIVELTGRNCNLIVVNSHNKILDALRHVDEFMSSVRRIMPASE
                     YAVPPLQNKKLINNVTIEDLDIYSEFSVSDAIFKTVMGTAPVFGKEVCYRAGVDDMKS
                     AKYLTDKEKEDLSAALSQIADISESYSYEPVAIYHDNKKNMKDFYCLNLSMYENIQSI
                     KVKRFDSFLDVFTQYIGEKSRVYELSQKSDTIKKILTAHLGKVEKKLNIYQNSLSDAD
                     KVKQYKLYGELIMANLPMLKKRAENVQLLDYYTNEMIDIPMDPSKDALKNAQSYYKKY
                     KKGMAAYDYSIKAIKEMKKEKDYLQSVMHALLSCEKKQDIEDIRKEMIQQGYMKEKQL
                     RKKSNKKIETETALFRPIEYISSEGYEIYAGRNNTDNDRLTFGFSEAYDIWLHIKNMH
                     GSHVIVKNKEQTEQFVPDKTLTEAAMVAAFHSEGKDSGQVAVDYTFAKNVKKPKGSKP
                     GYVNYFNYYSAYVKADKEAVEKLRKS"
     gene            29577..31775
                     /locus_tag="PLQ68_02890"
     CDS             29577..31775
                     /locus_tag="PLQ68_02890"
                     /inference="COORDINATES: protein motif:HMM:NF013316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HXK71628.1"
                     /translation="MELLSPAGNMQSLKAAVQNGADAVYLGLKTFSARANADNFTFEE
                     LKEALSYCHLRDVLVYVTINTLIDDSNMSFALDNAYKAYTYGVDAVIVQDLGLAAQIR
                     KNIPSLRLHASTQMSVINPKEAILLKNMGFKRIIAAREATIEQIKALTETGIEIEVFG
                     HGSLCVSYSGQCELSFFNGGKSGNKGGCAQPCRLPYTLEKEDKVLAFKNLLSTKDIAV
                     MDHIKQIENANVVSLKIEGRMKSPEYVAIITRTYKKAISGTLTQKDRQDAMMVFNREG
                     FSEGYMFSKPGKEMMAYNFSGNTGIKIGTVIGVNKEKGLIQIESDKELVNGDGISFPK
                     NDLGMYVNIIRKNGNKYGLICRNGVPDNFEHVYLTYDKQLMEEAQKSYSDDFYRKTAI
                     KGVFQAKHGEKAKFTVTDNRSNSFTAYSDIAVQEANSPDANQNVLKNLYKTGNTVFEF
                     EDIYIKTDFDVFLPAKTVNALRRDALEGLTEIRKAISVVPVDKVFPKHLNINRSINRT
                     DKKPGVSLFFFEDRPDFDIKDIFADRIYMPESMYKRYSYDNRAYMYKPIGSMLDEKTI
                     LPKRVISSSMGILEYDCDKVMDFGINCLNTYTADFLAGYNNVKAVCLSHELPIEDISS
                     FTGMNSNISFEYIAYGRVAVMKTKYCIKGAFNQIPECINQDLHLLDRQKKRIDIVTYC
                     DCHTSYLLGAFTINNINRIDEIRKSGADTLRINVYRETKEEIKEIIDKLL"
     gene            complement(31776..32423)
                     /gene="rpe"
                     /locus_tag="PLQ68_02895"
     CDS             complement(31776..32423)
                     /gene="rpe"
                     /locus_tag="PLQ68_02895"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HXK71629.1"
                     /translation="MIIISPSLLASNFARLEDEVRKIEEGGAQYVHIDVMDGHFVPNI
                     SVGIPVVESLRNITDLVLDVHLMITDPDRYIDDFAKAGADIICVQQEACIHLDRTIRH
                     IKENGKKAAVALNPATDISVLKNVLHLLDMVLIMSVNPGFGGQSYIPYSTQKIKELRK
                     MADSMGLNLDIQVDGGVTLDNCQEIKKAGANVLVAGSAIFKSDDIKETIRQLKEL"
     gene            complement(32423..33310)
                     /gene="rsgA"
                     /locus_tag="PLQ68_02900"
     CDS             complement(32423..33310)
                     /gene="rsgA"
                     /locus_tag="PLQ68_02900"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HXK71630.1"
                     /translation="MEIGLVTKSVSGQYTVKTEKDEYICKPRGLFRKDGFKPLCGDIA
                     EFEITDISDKEGYIQKIKERKNSLIRPPVANVDTSVIVFSINNPKLDEFLIDLLILAS
                     RAHKIQSILCINKSDLADDDKLRLVMKEYEKCGCEIIVTDALHVDNLDRFSKVISSGI
                     TVFAGQSGAGKSTLINALTKTKSMQTGILSRKIERGKNTTRHCQLLPYMEGYIVDTPG
                     FSKFDPGLLEPEAIKDMYNEFDKYKLVCKFNDCKHINEKGCAVIKAVEENLIPIGRYN
                     RYKMIYNMSKEALKNRRGY"
     gene            complement(33311..35461)
                     /gene="pknB"
                     /locus_tag="PLQ68_02905"
     CDS             complement(33311..35461)
                     /gene="pknB"
                     /locus_tag="PLQ68_02905"
                     /inference="COORDINATES: protein motif:HMM:NF033483.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HXK71631.1"
                     /translation="MDPVILNNRYELLSRMGTGGMAYVYKAKDKALNRTVAVKTLKDE
                     YAQDKDFIKRFKDEALSVAKLSHENIVNVYDVGYDNNLNYIVMECVEGTTLSSYMESI
                     GPLEIKETIDISLQIFAALEHAHTNGIIHRDIKPQNILISKNGKIKVTDFGIAKAINA
                     QTLTTDNKTFGSVHYISPEQAKGGFLSSSTDLYSTGIVMYEMLTGKVPFDADTPIAIV
                     MKHLQDNPTPPIEIREDIPEELNRIILKSIQKKPSDRYETAADAMIDLKNLENIGNGR
                     TDLTLDFIDGEVVNQEDTMAIPSVTEDDLFLDEEQGIVKSNTGWVKRNTGNIKGQKQI
                     NFDLEYNVPLVYARGRKKKEKKYFLTTLVAILMVLSLATVFVVYAIDELAKVVVPEIK
                     EYEVLNYVTYEYDEIKKKLEEDFGIIVLMKEAYSDETPAGIIMDQDVEQGRIFKEGAT
                     NTITFTVSIGSNYVSVPECEGKDYRVVEKEIINIGLVPEKVEQYSTDIDAGIVISVSP
                     LENTQVKYGETIRIYVSIGSQYEKTTVPDLMGLTKEQAAARLSQFNLVMNIMSEEEIP
                     GKIPIITYQYPEAGSGIYEHSTIDVKLAVDTEGVIYEDIYAKYVFDPTIAEISNMQFP
                     LTLRFKLEHSDQSGSSQTSVNVADLSKLPYERDIRIPAKGETKLTVFIGSVPYKDPIV
                     YKYFDYYVPTPSPSDQPGDTPEGEPGLPDAPVEP"
     gene            complement(35464..36216)
                     /locus_tag="PLQ68_02910"
     CDS             complement(35464..36216)
                     /locus_tag="PLQ68_02910"
                     /inference="COORDINATES: protein motif:HMM:NF033484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HXK71632.1"
                     /translation="MIINYASYTDIGKVRENNEDYHIVIPFEEHNEFAAAVADGMGGH
                     LFGEIASKTAIDTFEKKIRDRRFKNCINIKDYLYNVCKLSNKEILEFSKKHNVLSGMG
                     TTLDAIYIKDDKIHIIHIGDSRIYTISGDNIKMVTKDHSYVQQLLDKGDITFDEFKNH
                     PNKNVITKALGITDNPEPDIFEMQYNFCGYMLLCSDGLTNMLDDNNILNIINQDTDLD
                     TKVRNLGEKANENGGTDNITIVLIKTEKKAEE"
     gene            complement(36206..37237)
                     /gene="rlmN"
                     /locus_tag="PLQ68_02915"
     CDS             complement(36206..37237)
                     /gene="rlmN"
                     /locus_tag="PLQ68_02915"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HXK71633.1"
                     /translation="MNSQKTHILSYTHNELKDLFQQYGLKRYRAIQVLEWVYSKKIFD
                     FSLMSNISKEDIALLTENFTLELPEIEKKLVSQDGTIKYLFRYSDGATVESVVMDYDY
                     GTSICVSSQSGCKMGCVFCASSKVPFSRNLEGYEITAQILKAASDSNKNISRTVVMGI
                     GEPFDNYDNLVNFLKIINMKEGLNLGGRRITVSTCGIVPKIYEFADLGLQVNLSVSLH
                     EVTDKERTAIMPINKKYNIEQVIKACEYYFEKTNRRVSFEYALISGKNDTDEHAMRLC
                     SLLKNVMCYVNVIPVNPIDKKSAHQNSKNLSGFCAILKENGIPVTVRRELGRDIDAAC
                     GQLRRQNDN"
     gene            complement(37290..38522)
                     /gene="rsmB"
                     /locus_tag="PLQ68_02920"
     CDS             complement(37290..38522)
                     /gene="rsmB"
                     /locus_tag="PLQ68_02920"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: protein motif:HMM:NF011494.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HXK71634.1"
                     /translation="MDSRHTALNIVHDVIYKDKYSNMVLESYMNKAKLSVEDRRLCMN
                     ISMGVLRNSIYLEHILKPYIKNIKKTSPLLLCILKIGIYQFVFMDKIPQYAIVDESCN
                     LAKKVVSKNSAGFVNAVLRNFIRKNKVEDSNIEPHILYSCTKETYKAMRSLLSEEQCI
                     EMLKKFNSPQDTYISINTHFHNKDEVIYNMQKQGFDIEITDNTSILVKGNSSPSSSNE
                     YSKGAIIVQDELAVQIAKLLNVQNNMTVLDACASPGGKTIYLANTMENTGNITACDIV
                     QSRVSKTVENIERCGFTNISCFVKDMTEYFPEYENAFDRVLIDAPCSGLGVAGKRPEI
                     KLKYKTSDSLTKTQKALIKTCSRYVKKDGYLVYGTCTINRQENQDIVEGFLSENNEYG
                     FEEDFFDQGFYAARLIRK"
     gene            complement(38522..39469)
                     /gene="fmt"
                     /locus_tag="PLQ68_02925"
     CDS             complement(38522..39469)
                     /gene="fmt"
                     /locus_tag="PLQ68_02925"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HXK71635.1"
                     /translation="MKKISKIIFMGTPDFALPCLEALIESGYNINAVITQPDRPKGRH
                     NVIEFSNIKKTAIKNNIPVLQPEKIRKDNWPQRISEIGCDLAVTCAFGQILPKSILEI
                     PKYGTINVHASLLPKFRGASPMQRAIIEGEEITGVTTMLTDVGMDTGDMLLKKETNIT
                     KEDDICSLHDRLSIMGSNLLIDTIKLYEQDKIVPVKQDDSLATYAPMIKKEEGKLDFN
                     QDALRLFNVIRAFNPWPGTYTSYCGKTMKITKAEYSDDVNDSSIANGTILNIEKDKMA
                     VKCESGILYITELQFEDRKRMQLCQCGHNLTCGITLGGC"
     gene            complement(39453..39947)
                     /gene="def"
                     /locus_tag="PLQ68_02930"
     CDS             complement(39453..39947)
                     /gene="def"
                     /locus_tag="PLQ68_02930"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016427735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HXK71636.1"
                     /translation="MAIRKIRIEGDDILRKRSREVTSFDDRLHTLLEDMYETMVAAPG
                     LGLSAVQIGVLRRVAVIEMDEKKYEIINPIIVEQEGENIDIEGCLSIPEYYGTVKRPL
                     KVKIEYQDRYGNKQVVEAEEYTAHCFSHEIDHMDGILFRDLVIEEIDPSELEGNEEEI
                     EEDK"
     gene            complement(40050..41174)
                     /gene="ugpC"
                     /locus_tag="PLQ68_02935"
     CDS             complement(40050..41174)
                     /gene="ugpC"
                     /locus_tag="PLQ68_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HXK71637.1"
                     /translation="MAKVTLRNVYKKYEPNRGGITAVSDFNLVIEDREFLVLLGPSGC
                     GKTTTLRMIAGLEDITAGEIYIGDRLINEVHPKDRDIAMVFQNYALYPHMTIFDNMAF
                     GLKLRKMPKAEIRQRVHSAAKILEIEHLLDRKPKALSGGQRQRVALGRAIVREPKVFL
                     MDEPLSNLDAKLRAQMRVELTKLHQKLKTTFIYVTHDQVEAMTMGSRIVVMRAGFIQQ
                     VDPPQIMYDNPSNMYVAGFIGTPQMNFINAVIEEKGNDVWINLAGTKIKFSEKRAKSI
                     VGKGYDGKEVVLGIRPEHFRDEQVYLDQMTDTTIEVKVDVVEMMGPEALLYFSFAKSN
                     IIARVSPSTKARPGSTIKIAIDTIRIHIFDKESEKTIFAE"
     gene            complement(41291..41896)
                     /locus_tag="PLQ68_02940"
     CDS             complement(41291..41896)
                     /locus_tag="PLQ68_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009003759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HXK71638.1"
                     /translation="MTECKLKLMTTKNMAKMGILGAVAGLLMLLEIPLPFAPSFYEVD
                     LSEVAVLLGAFSMGPVAGVIIEAIKILINFVITGTATAGVGELANFIVGCCLIVPAAM
                     IYNKNKRIKNAIVGILVGIFAMTLVGAILNYYILIPVYASAFGMPIETIVAMGTKLNS
                     NIDSLWDLILLANVPFNIIKGILSGLITFLLYKRLSPILHK"
     gene            42138..43103
                     /locus_tag="PLQ68_02945"
     CDS             42138..43103
                     /locus_tag="PLQ68_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012060994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HXK71639.1"
                     /translation="MKLGVFTVVLGSMNLEDTIRYLKDLGVSAIELGTGGYPGTAHVN
                     AKEIFDKPDEIKKIREVVDKYDMQISALSCHGNPVHPNKKIAQDFHDDFEKTILVAEK
                     MGIDTVVTFSGCPGDYEKAKYPNWVTCPWPDDFLKILEYQWNDVLIPYWSKQVEFAKK
                     HHVTKIALEMHPGFCVYNPETLLKLQRAVGPVIGANFDPSHLFWQGIDVIKAIRELKG
                     SIYHFHAKDTKIYYPNSEVNGVLDTKPYKNESERSWLFRAVGTGHDRQVWNDIFAELR
                     MAGYDHVISIEHEDSLMSGQEGLKKAIAFLKETMIFEPKGEMYWA"
BASE COUNT        12447 a         8623 c         6478 g        15148 t
ORIGIN      
        1 gacctcttga atgccattca agcgctctcc cagctgagct atacccccga ctttaaataa
       61 tatactatct ggtagctgga aatgtcaatt gttaattaca tttctaatga agggtctgcg
      121 tttatgtcaa agtcttcttc ttttatgtag tatgcagctg agccaatcat agctgcatta
      181 tccgtacaaa gatgaatatc tgggaaatat acttgcactt tttctttgtc tgctctttca
      241 tataatgctt ttcttaatcc ttgatttgca gctactcctc ctgctacaac tattttttca
      301 cttttagtta gaaaaacgtt ttctaccaag acatcagtta ctgctttttg aaaagatgct
      361 gcaacatctt tttttgagta ttcctcattt ttctgttctt ttgaatgtat atagtttata
      421 accgctgtct tgagaccaga aaagctaaaa tcaaatggat tgtccttaaa ttttgtccga
      481 gggaacaaaa tggcttcagg attgccctca attgccattt tatcgatttt tggtccgcca
      541 gggtaaacaa gacccattac tcttccaacc ttgtcatatg cctctcctac ggcatcgtct
      601 cgggtactac cgatattttg aatatcatca taattattca cattaagtat attggtatgt
      661 cccccggata ctaaaaggca cacaaaagga ggtttaaggt ctttgtgtgt gatatagttt
      721 gcagcaatat gaccttttaa atgatttact ggtattaagg gaatatccaa agctgctgct
      781 aaagcctttg cagctgtaat acctacaagt aaagctccta ctaagccagg tccttgagtt
      841 actgctattg catctagttc ttcttttttt atacctgctt ttttaattgc ctggtctata
      901 accatcaata tatattcaac atgttttctt gatgctatct cgggaactac tcccccatat
      961 tcctcaaaaa tctgagctga agaagctata atattggaca ttactattct tccgtctttt
     1021 acaacggaag cagcagtttc atcacaactt gtctctattc ctaaaattat cataatacaa
     1081 tgttaaatac aaactggtct aatatacaaa agattcctaa gatagctacg tagtaagcaa
     1141 aatactgtat tttaccttta aggactgtct ttacaaaaat tttgatagcg aaaaacccgc
     1201 ttacccctgc aaatatcata ccaaaaaata tgggaagtat ttcaatattt tcaaaccctg
     1261 cttttacagc atcataagtt tccaaaagga atccccccag tattaccggt attgaaagca
     1321 aaaatgcgaa ttttacagca tcttcctttt tgacttttct aaaaagccct ccagtaaggg
     1381 tagatcctga ccttgaaata ccaggaacta ttgcaaccat ctgcatgata cctatgaata
     1441 aagcgtcata ccacttcata ttttcttttt ccatggttcc aggttttagt tttgatgata
     1501 ttatcaaaaa acatgctgta atcaaaaaac cgattcctaa agtaactccg ctatcattga
     1561 atccttcaaa aagatcattg aataatactc cggctatcgc agcaggaatt gttgcaataa
     1621 tgagccataa tgtaagtttc tgaataggct ttttcaatat ggcaagtata tctttccaaa
     1681 atactgcgca aacagatatt aaagttgcaa aatggacagc cacattgaaa gttaaagtgg
     1741 gagtgctgat attaaggatt ttttcaaata atacaagatg tcctgaactt gagactggca
     1801 gaaattcggt aaaaccttgt ataatcccta atagtacagc ttcccagata ctcatgatgc
     1861 gctttcttct aaaagttttc ctctgtaaat caattttgga ggagtgcagt tcaatatagt
     1921 gctttcctcc actatacact tttctatagt tgggtctgat ggcacgttat acataacctc
     1981 catcagtact gattcaagta tggatcttaa tcctcttgct ccgctatttc tctcaagagt
     2041 ctttttagca atagcataaa gagctttttc ttcgaatata agctcgcatc catccatttc
     2101 cattatcttc ttgtattgct ttgtcagagc attcttcggt tctttaagta ttgataccaa
     2161 gatttcctct gtcagctgct ccaatgtaac aacaacagga agtcggccta caaactcggg
     2221 tataagtccg tactttaaga gatcctgatt ttcaatatgc tttaaaactt tatctttctc
     2281 aatacttttt gaagataaaa tttctgctcc aaagccgata gctcttttgc ctaatctatt
     2341 ttgtattatt tcgtctatac cctgaaatgc gcctccgcat atgaaaagta tatttgttgt
     2401 atctatctgt atgtattcct gagttggatg ttttcttcca ccctgaggag gaacgttagc
     2461 aaccgtacct tcaagaatct tcagtaatgc ctgctggaca ccttcacctg atacatctct
     2521 ggttattgat gggttgtcag ctcttcttgc tatcttgtca acttcatcga tgtaaatgat
     2581 tcctctttga gctcttttta catcataatc tgcagcctga attaatctta gaagtatgtt
     2641 ttccacatca tcacccacat aaccagcttc agtcagggtg gtagcatcag aaatagctat
     2701 gggtacattg agtatctttg ccaaagtctg ggccatataa gtttttcctg tacctgtagg
     2761 tcctaacata agaatattgc ttttctgaat ttctatatca tcttcaggca ttgaggttac
     2821 attatggctt agtctcttat aatggttgta tacagcaaca gaaatagctt tttttgcatc
     2881 atcctgacct ataaggtaag catcaagtct tctctttatt tctgcaggac gtggaacctc
     2941 atcaatcatt ggttctttat ccagttcctc aacatcatca tatccgaatt cgctgtcaag
     3001 taaatccgaa cacaaatcta tacattcatt acatatatat acattcggac ctgagatcag
     3061 tctttgtacc tggtcttcgc ttttaccgca aaaagagcat ttgatcttct ttatttcatt
     3121 tgacattcaa atctccttac tatcttttaa caagaacttc atcaattatg ccatattctt
     3181 ttgcatctgc tgatgacata aagaagtccc tttctacatc tttctgaatt ttcttaagag
     3241 attgtccagt tttatcagct aatatcctgt taagattttc tttaaccttt aataaccatt
     3301 ctgtatgaag gcgnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnntctccta
     3421 agaatactat cctatctttc aacaaacgtg aaaaaatatc atatgacctt tcacctctgc
     3481 tggtctgttc aataacatat ggtatcgtat tcattttgta tccttcctta acagttattc
     3541 tttatctgtc ttatcttctt cggctttttt tgtgctcttc ttcggagcgg cctttttctt
     3601 gggttccgct ttttttgctt cgccttcttc tttattatct ttcttttctg tctgcttttt
     3661 agctgcttct ttcttagcct ttacttcttt tactttcttt gcagatgcat atataaagtc
     3721 tattgttttt tcatctgcta ctctgtctgt gtaatacttt ttgtaatcac cgatagcact
     3781 ttttattttg tcaacttcca tcttgtacat ttcagcttct tttttaagct cttcttctat
     3841 ctcttcgtct gtgacttcaa tattttcgga ttttcgtata gcttcaatta ctaaatcacg
     3901 gactaccatc atttttgctt gttcagcata ctgcttgcgt acatcctcaa gtttcattcc
     3961 tgtatattca agatactgtt cgagtgtgta accgctatac tgcatttcca ttttcatatt
     4021 atcaatgatt ctgtctgttg cttgctcaat cattgcatta ggtatatcag cttttgcatt
     4081 ttcacatgct actgctagtg cctgagaacg cattgaatct tctgcttttt tctgcctatc
     4141 ttcaagaatc ttctttttag tctcttcttt gtactgctca actgtatcgt attcgcttat
     4201 atcttttata aactcgtcat caataactgg gagttcctta acttttattt catgtagtac
     4261 tactttgaat actgcatctt ttccctttaa atcctcagag tgatattcat ctggaaattt
     4321 aacattaaca tcaaactcat cgtttatctt atgaccgatt atctggtctt caaatcctgg
     4381 tatgaatgat tttgatccta ttacaagttc atgcttttca tcttttccac cttcaaaagc
     4441 aactccgtca acaaaacctt cataatcgac tataactgta tcctgttctt gtacaggacg
     4501 atcttctata ctaatcatac gtgaattctt ctcagcttca ctattaaggg catcttcaac
     4561 atccttatcc gttacttcaa caacagcttt ttgaatttcc acacctttat attcacctaa
     4621 ttcaacatca ggttttacat caaccttaat tgtaaaagtg attccgctct cttcatccat
     4681 gtcttttaag tcaacattag gataatccac tacttctaat ttcaaatcat caatcgcttt
     4741 tggatattca gtattaagag acatatctat tgctgtatca taaaatacgc cttttccgta
     4801 tacacgttca atcaaatact gaggagcttt tccttttcta aaaccctgaa ccgtaaactt
     4861 gcttttatct ttgttatacg ctttcgtaat atatccttca aattcctctt ttgtaagagc
     4921 gatttccaat tctactgtat agccttcttt tttaactatt ttattctcca ttatatacct
     4981 ccgcatcttt gcgctattgt ctataatatc atatggcaaa tactaaatca ataatattat
     5041 actccgttat tacatgatat gtaatatttt aacagttatt ctccagcata ttgtgattga
     5101 ccgatatata aagttatatc ctgctcaata atctggtctg catacttaaa taaatcttta
     5161 cataaggaat cgtaatacaa atccataacc gaatcagaaa aatcataacc aaaatatgta
     5221 cccttcggaa ttgaatatgt aatatattct tcttcctcat taactgtatt aataattgtt
     5281 acacttattc tgtcttgtga ttgaatgtat gattctaaaa tattattatt aattagttca
     5341 tttccatatg tgaatgtttt agtcattaaa gtcagattat ccccgccttt tacatcaagt
     5401 ttgaagtcgt gtgatattat ctgccagtca ttatctaaaa catatgttga taaaattgtc
     5461 tcacctttgg aaagattcca caacgagaac tcactggtca gaatatcatt aatctcaaat
     5521 gaataaacta tttctcttaa acctttatct gatttatccg acacgagttt gtaatttgca
     5581 taattgttaa aatcaatatt ttcaaggtaa ccagaagtat atgcatcata aaccaaatct
     5641 gacataataa cagtataaaa gtcctcatta tttacaagta tgttcataat cccatcaaag
     5701 aagtatgaaa tcctgtcctg ttccgaatta tctacaattt ctatcttatc atcattcatt
     5761 cggccagtca tagaatatgt cacatcatca tatccgtaca tattcnnnnn nnnnnnnnnn
     5821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5881 nnnnnnnnnn nnnnnnnnnn nnnnngattt ttcagttttc acatatgagc atcgtattgt
     5941 tccaagcatg ctttcgtata ctgcatcaac tttttcaagc ggagtattaa accctggagc
     6001 cagagtgaat cttttaaatc ccggatattc cgtatctggc tgaattcctg caaagtattt
     6061 gaaaaatgtt gctgatatgt ctccaaacat atgatgattt cttgaaccct caccattcca
     6121 acattcatat agagttgttg caccaagttc ttttatgcaa ttagcaaaac ttgggaatgt
     6181 atcctgtgta atcattgaat aaattacatc agcacgaccc ttcttcccta atatgttatt
     6241 gacatattta ttacccaaaa ttccataatc gagatgtcca tcggcttcat taattgttct
     6301 gatcaaatgg ctgtaaataa ggtcatattc cttttcattt tcataaagat tatgataaag
     6361 cattgctgca taacaggttt gtccatcacc ttttagcctg ccagtaagta catcaaagaa
     6421 agcccttctg aagcctgctt ttattctctt ggcaatttta gagaattctt ttgcatcttc
     6481 tctaattcct accatcttag caaatttttc caatacatta gccatagtat acatacaggc
     6541 tgtgtcagta aggtcaattg gagctttaaa tgaggacata ttgacagaaa tagcaggacc
     6601 ttcaaaaggt gcacaccagt ctcctactcc atagtcattt acacatacat cgtcctgcat
     6661 cattgagagc atatagaaca tattacgttt catgtattcg taatacttgc atatcataac
     6721 cttgtctgag tagtatgtat tcatgtggtc tggaagccat gtcagtactg atgaataatc
     6781 tggaccatta ccccagttat agccccagcc agctgtaggg accatgcaag gaatatttcc
     6841 ttttgctttc attgaatctt taatatcatt aagccatttt atccaaatag gaccagacat
     6901 ataattaaac aacatctgtt cggtagaaac attcgtatca ccagtccatg cattcttctc
     6961 gcgatgagga gaatctgtaa gcagaccata catatttgag taagtagcca ttctggtatt
     7021 cttctgtatc tgattaatta tctcgtcaga acatgaaaac tggcctatct cttttactgc
     7081 agtatgtaaa aatacggccg ttgctgcaga tgtgtccggc ttagttgcaa atccttctat
     7141 ctgagcatat ctgaatccnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnca
     7261 tctgcatcac accagttagg tatttcaagt cttgcatcat aatactcacc atttctaagg
     7321 ctgttaaagg tgatagggct catatgactt tcccatgtta tatcagaagg tatacatatc
     7381 tgcttgttat cctttgttgt aatatgtaac tcacagatta atttcggact tcctttccat
     7441 gaagcagcct gtgtactcca ctggtctgtt gtaggaagat taaaaaaacc gttacctaaa
     7501 attactccaa gcatattact gccctttttc aaatattcag ttacattaaa tgaataatag
     7561 agcagtgttt tatcatatgc ggtatatggt ggatccataa tatggtcatt tatctttttc
     7621 ccatttacat aaggttcaaa aaaaccagcg ccagctatgt atagcattgc ttttgaaaca
     7681 tcattattta cgttgaatct ttttctcaga tagggagacg aagccataag ctctcttgga
     7741 accttaaatc cgctctgaat aaatcttcct tcccactttt cttctaattt tccagtatca
     7801 aagaagccat cttcactttg agctctctca ccggatttat cagttatact cacgttccaa
     7861 cgatatgtcg ttcttggcat gagttttttc ccctcatact caattagaac agattcatct
     7921 gatcgaatat ttttagaatc ccagacaagt tttccacctg tcataacctt tattctgtaa
     7981 gaacgctgtc tgtttcctct gccgtcttgt ataagtttcc atgagaatct tggaacctga
     8041 tcaattccaa ctgggttttc cagataattt actgttaaat ccttgattgt gatagacatg
     8101 ttgtttctcc tgacttagtt aattaataat ctaattatat ctttttatag aagatataac
     8161 aaggtgaagg gaaataaaga aaataaagaa aataaagaag ataaagaaaa taaagaaaaa
     8221 acagaaaaaa aaagaaaaca aaaaacaaag acaggtataa acctgtctct gatgaattta
     8281 agttatatta ttaaatctta cttaaatatc ttagcaacgc ttcctgcgcc tactgttctt
     8341 ccaccttctc taatagcgaa tcttaagcct tcttccatag cgattggtgt aataagagaa
     8401 atcttcatgt ctatgtggtc accaggcatg cacatttcaa taccatcagg aagattagca
     8461 acaccagtaa cgtctgttgt tctgaaatag aactgaggtc tgtaacctgt aaagaatgga
     8521 gtatgtcttc caccttcttc agctgtaaga acgtatactt ggccttcaaa ttcagtatga
     8581 ggtgtaattg atcctggttt agcaagaact tgtcctcttt cgatatctgt tctctgaacg
     8641 cctcttaata gacatccgac attatctcca gcttgtgcga aatcaagaat ctttctgaac
     8701 atctcaacac cagttacaac tgttgaagaa actgtttctt taagaccaac aacttcaact
     8761 gtatcctgaa ctttgattgt tcctctttca actctacctg tagcaactgt tcctctacct
     8821 gtgattgaga aaacgtcctc aacaggcatt aagaaaggtc tgtcaactgc tctttcaggt
     8881 gtaggtatgt atgaatcaac agcttccata agcttaagaa tagattgata ttcttctgaa
     8941 gtctgatctg ttgatgttga ttcaagagct ttaagagctg accccataac tatagggata
     9001 tcatctccag ggaattcata ctctgaaagg agttctctta attccatttc aactagttca
     9061 agaagttcag gatcatcaac ctggtctgtc ttgttcatgt atacaacgat ataaggaacg
     9121 ccaacctgac gagcaagcag tatgtgctct cttgtctgag gcattggtcc gtcagctgct
     9181 gaaacaacaa gtattgctcc gtccatctga gcagctcctg tgatcatgtt cttaacatag
     9241 tcagcatggc ctgggcagtc aacgtgcgca taatgtctgt tttttgtctc atactcaaca
     9301 tgagctgtag caatcgtgat tcctcttgct ttttcttctg gtgctgcatc aatttgatcg
     9361 tatgctttga aaacgatatt tttatcagct aaagctaatg tctttgtaat agcagcagtt
     9421 aatgtagtct tgccatggtc tacgtgtccg atagtaccaa tattgcaatg tggtttagtt
     9481 ctttcaaatt ttgcttttgc cattttattt ttttcctcca attataatga gctggttaaa
     9541 tattaatcat atacattcta catatgaatg catattgtgt caatagttgg ttttgaggat
     9601 ttttgcagcc gtttcgtcag gaaccttctc aaaatggtca aattgcatag taaatgtgcc
     9661 tcttccctga gtatttgatc tgaggtctgt tgcatatccg aacatttctt ttaaaggaac
     9721 cacactagta atgattgact caattcctct tgcttctaca ttttcaattt ttcctctttt
     9781 tgatgttata tcacccaaaa catccccaag ataattttca ggtacgaata catcaacgcg
     9841 catgacaggt tctagtaaga tagggtctgc tttaaggcat gcttccttga aagccatgct
     9901 tccagcaatc atgaatgcca tttctgaaga atctacttca tgatatgatc catcaacaag
     9961 cgtcacagca acgtcaacta taggatatcc ggcttttatg ccattaccta aagattcctt
    10021 tatacctttt tcaacagctg gaatgtaaac cttaggtatt gcacctccaa cagtcttatc
    10081 agtaaatgtg aatccatcac cttgctttgc aggctctata tcaagaacta catgtccata
    10141 ctgtccgtgt ccgccagttt gtctgacgaa ttttgactct ccacgcatag gtttggttat
    10201 agtttctttg tatgttacct gtggtcttcc ctttgtcgca tcaacctgaa actctcttct
    10261 cattctgtca actaggatat ccagatgcag ttctcccatt ccggaaataa tcatctgtcc
    10321 tgtatctgaa tcggttttgt atttgaatgt aggatcttca tcagaaagtt tttgtagagc
    10381 tgatgtcatt ttctcctgat ttgctttatc ctttgcttca acagaaatag agataacagg
    10441 ttcaggaaac tgcatagact caagaataac tggatctttt gcatcacaca aagtatctcc
    10501 tgttacagta aattttaaac ctactgcaca tgctatatca cctgaatata ctttatctat
    10561 atcagttctg ctgtttgagt gcatcattag tattctgcct atacgttcct ttttattctt
    10621 agttgcatta agaacgtatg agcctgtgtt cagtgttcct gaataaactc tgaagaagca
    10681 tatctttccg acataaggat cagacattat tttaaatgct aatgcagaaa aagaatcgtc
    10741 atcatctgcc ggtttagtta taactttact gtgatcattc atatccacac cttcaatagc
    10801 atgaacatct atcggtgaag gcagataatc ggtaacagca tcaagaagct tctgaactcc
    10861 tttgtttttg tatgatgatc cgcatattac aggtgttatc gtaagtttaa ttgttcctat
    10921 cctgatagct tttttgattt cctcatcaga aaccgtctcg ccttcaagat atttttccat
    10981 tagattttca tcttcatcac aaaccgactc aatcaatctg atacgataat ccgttgcaag
    11041 ttccaacata ttttcaggaa tatctctttc ttcaatatta ttacctaaat cgtccatata
    11101 gtaataagct ttgaacttaa caagatcaat tattccagta aatgttgatt ctgcacctat
    11161 tgggatttgt aatggaatag catttgcatt gagtcttttt ttcatcatgt cgatacaacg
    11221 ataaaagtca gcaccggtag tatccatttt attaatataa gcgattctgg gcactttgta
    11281 tctgtttgcc tgtctccata ctgtttctga ctgtggttct actccgcctt ttgcgcagaa
    11341 aactgacaca gcgccatcta gtactcgaag gcttctttca acttcggctg tgaaatcaac
    11401 atgacctggt gtatcgatga tattcaagcg gtaatctttc caataggctg tagtagctgc
    11461 agaagtgatt gttatacctc tctcctgctc ttgttccatc cagtccatcg tagctgcgcc
    11521 ttcatgaact tctcccatcc tgtgcagctt tcctgtatag taaagtatac gttcggtggt
    11581 agtagtcttt cccgcatcga tgtgggccat tattcctata tttcttgtga attccagtgc
    11641 atagtctctt ggcatattat ctgttcctgc ttaccatctg taatgagcaa atgccttatt
    11701 ggcttctgcc attctgtgca tttcctcttt tctcttgaag gtatttccga ctccgtttac
    11761 agcatcaagt atttcagctg caagtctttc cttcattgta cgctcgtttc ttgatctggc
    11821 aaactgtgta agccatctta atgcaagagc ttgccttctc tccggacgaa cttccattgg
    11881 aacctgatat gttgctccac caacccttct ggcttttact tcaagtgcag gcataatatt
    11941 gtccattgcc tgtttgaaaa cttccagcgg gtctcttcct gtttttgtct taacgatttc
    12001 aaaggcatca tagcatatct tttgagctac accttttttg ccgtcaagca taatattgtt
    12061 tattaatttt gttactacct tatccttgta taaaggatcg gctaacactt ctctctttgg
    12121 gacatgtcct tttcttggca cttgtcttcc ctccttaata ataatttttc ataggtactc
    12181 aaccatttca gctgtcagtg ccggtacata tcacttttta agtatatatt aagtacctga
    12241 cacttcaatg tacgttattt accttttttt gcaccatact tggatctgcc ttgctttctg
    12301 ttagcaactc cttgggtgtc caatgttcct cttatgatgt gatatctgac acctggcaaa
    12361 tctttaactc ttccgcctct gataagaaca acactatgct cttgcagatt atgtccgatt
    12421 cctggtatat atgatgtcac ttcatatcca ttagtaagcc ttactcttgc aattttcctc
    12481 aaagctgaat taggcttttt aggtgtgctt gttttaacta ctgtgcaaac acctcttttt
    12541 tgaggacaag caactgttgt tgttctcttc ttaagagtat tcatgctctt ttgtaatgca
    12601 ggtgagagtg atttcttgtt tgaaaccttt ctgccgtttg atactaattg attaaatgtt
    12661 ggcatctttt tcctcctttc ctttataatt tatatattta cttcaaaata catactaccg
    12721 ttgcgcatac acttatgccg ttcaaaagac cgagttctct agatgtttta acatactcta
    12781 catctatttc cttcttctta cacatctcta aaaccggacc ggtcaaagaa ctatttgcat
    12841 cttctgcaac atacacaagc tttgctctgt tttgctttat ggcttttact gtttgcttca
    12901 ggcctataac cttattctca tgtacagcca tttataatcc tattccttct cttcttcctt
    12961 ttcttctggt tcattttcgt catcagaatc ggattcttcc aaaaggcctt cattatcata
    13021 ctcttcggaa tcgttttcgt caaggttttc tacttcatct tctaaatcat caaaaagatc
    13081 aaactcttca tcaaatgctt cttcagaaat catttcttcg tcctctgttg gatccatttc
    13141 aagatacgga tcctgataag cgttcattcc tgtacctgct ggtattagtt ttccgataat
    13201 aacattctct ttcaggccta taagattatc agtcttacct ttgatggatg cttctgtaag
    13261 aactctggtt gtttcctgga aagacgcagc tgataagaaa gattctgttg ctaatgaagc
    13321 cttacttatt cctagaagaa ctctttctcc tgttgctggc tgcttgttat tctccagtgc
    13381 aatttcattc tgatcttcaa actctgtagc atcacagtaa cttcctggta taagatttgt
    13441 gtcaccacag tcttcaacct taaccttgcg aagcatctgt tttacaatta tctcaatgtg
    13501 cttgtcattt atatcgatac cctgaagttt gtaaactttc tgaacctcac aagcgatata
    13561 tctgtgaact ccatctactc ccttgatcct taatatgtca tgaggattct cagaaccttc
    13621 tgttatctta tcaccttttc caatttcatc accatcttca actgtgagtc tggatccgta
    13681 tggtatagta tatgtatgtg attcatcttt actagttact ataacttctc tgcgtttctt
    13741 ggtttcgtta attttaacaa taccttcggt ttctgcaata attgcaagtc cttttggctt
    13801 tctagcttca aacaattctt caacacgagg aagaccctgg gtaatatcat caccagctat
    13861 accacctgtg tggaaggttc tcattgtaag ctgtgtacct ggttcaccta ttgactgtgc
    13921 tgcaataatt ccaactgctt ctccaatatt tacaggctta cttgatgcaa gattcattcc
    13981 ataacattta gcacatacac caagatgaga tttacatgtc agaactgacc ttactcttac
    14041 ctgcttgata cccgcttcta ttatcctgtt tgctgcatca gcgtcaatat acccgccagc
    14101 ttcaactata acttcattct tgtcatttgg attaactatc tcttttacta catatcggcc
    14161 ataaattctt tcgcgaagtc ctacaactat ttcaccactg tcaacaacat catagacaag
    14221 aatatcatct tcagatccgc agtcttcatc cctaactatt acatcctgcg aaacatcaac
    14281 aagtcttctt gttaagtatc ctgagtcagc tgttcttaat gctgtatccg caagaccttt
    14341 tctcgcacca ttactggata tgaagaattc taaaacattc aatccttcac ggaagttaga
    14401 cttaattggt atttctattg tcttaccaga tgtatcgaac atcaggcctc tcataccggc
    14461 aagctgctta atctgatttg tatctcctct tgctcctgat ctgctcatta tgttcaatgg
    14521 attaaatgca tccaacccat ccattagcat ttttgttaca ctatcagtag tatctctcca
    14581 taacttgaca acctggtcat gtctttcttc atctgtaatc aagcctcttc tgaaatcttt
    14641 tgtaacctta tctactttta tttctgtttc tgcaagtgtt tctttcttcg aactaggtat
    14701 aatcatatcg cttacagata ttgatatagc tccctgagtt gaatacttaa atccaattgt
    14761 tttaatttta tcaagcactt ctgctgtttt agctatacca tgctttgtta tacatctgtc
    14821 aatgattttg gataggtctt ttttgccaac catctgatct atttccaaat caaacattgt
    14881 ttcaggatta cttctgtcta caaaaccaag atcctgaggt atagcttcat taaatataac
    14941 ttttccgcaa gttacattta taattctgct ttgtatcttc ccgtcaattt ctttatatgt
    15001 tctcattttt actttggaat gaagtgtaat tgtcttttca tctaaagcaa gtagcatttc
    15061 gtcgaaatca cggaatacgc gtccttcacc gacttcaccg tctttagctg tagtcagata
    15121 atatcctccc aaaaccatat cctgtgtagg aactgtaatt ggtttcccgt cttttggtgc
    15181 gagaagatta tttgatgaaa gcatcaatac tcttgcttcc gattgagctt ctgcagaaag
    15241 aggcacgtga acagccatct gatctccgtc gaagtcagca ttatacgctg tacaaaccag
    15301 tggatgaagt ttaatagctc ttccttcaac caatattggt tcaaaagcct gtattcccaa
    15361 acgatgtaac gttggcgctc tatttaaaag taccggatgg tcttttataa catcttcaag
    15421 aatatcccat acttcatgac gaattctttc aaccattttc tttgcacttt ttatattttg
    15481 tgcgaattta tcggtaacaa gacgcttcat tacaaatggt ttgaaaagct ccagtgccat
    15541 ttcttttgga agaccgcact gatacatttt taattcaggt cctacaacta taactgaacg
    15601 accagaatag tcaactctct ttccaagaag gttctgtctg aatcttcctt gcttacctct
    15661 gagcatgtca gaaagtgatt ttaaaggtct gttgccaggt ccaacaaccg gtttgccttt
    15721 tcttccgttg tttatcaaag catcaacagc ttcttgaagc attctttttt catttctgac
    15781 aataatatca ggagctgtga gttcaagaag tctctttagt ctgttatttc tattgataac
    15841 tcttctataa aggtcattca aatctgatgt tgcaaatcta ccgccatcta actgaaccat
    15901 aggacgaaga tcagggggaa taacaggaat agtatctata atcatccact caggcttatt
    15961 ccctgatact ctgaaagact ccatagcttc aagtctttta attattctgc ctcttttttg
    16021 acctgtgctc gattgaaggt ctaccttcaa ttgtgcactt tcagactcaa catcaattct
    16081 ttctaataat atctttatgg cttcagcacc catttttgct acaaaagaac ctctgccata
    16141 tttgtccagc atttctctat actgcatttc agttaaaaga tctttaacct cgatagtctt
    16201 gtcattaact ttaacgtctt cgttattaac atccagaacg atataagaat taaagtataa
    16261 aactttttct aaagatctcg gactcatgtc caaaaccagc cccattctac tggggattcc
    16321 tctgaaatac cagatgtgtg aaacaggagc agcaagctct atgtgaccca tacgttctct
    16381 tctgaccttg ctttttgtga tttctactcc gcaacggtca catatgatgc ctttatgcct
    16441 tactctttta tactttccgc aatgacattc ccagtctttg gtcggtccaa atatcttctc
    16501 gcagaaaagt ccgcctgctt caggtttaag tgtcctgtag ttaattgttt caggtttctt
    16561 tacttcgcct cttgaccact ccttaatcag ctcggaagaa gccaaaccta ttttaatcat
    16621 gtcgaaattt gcgtaatcct gcattatcta ctctcccatg tcatctaaat caactatatc
    16681 ctttacattg gatatctttg aataatctgt gctggtgtca tcttcatcag catcttttaa
    16741 taccatattg gatatgtcat ctgaatcaat atcttcatca tcttctannn nnnnnnnnnn
    16801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16861 nnnnnnnnnn nnnnnnnnnn nnnnnnnggt atactttcgc ctttaacaat agcttcatat
    16921 gtttttactc ttccaattac atcatcagat ttaaccgtaa gtatttcctg taaagtatat
    16981 gcagcaccgt aagcttcaag tgcccaaact tccatttcac caaaacgctg tccaccaaac
    17041 tgtgctttac cgccaagagg ctgttgtgtt actaatgaat atggtcctgt tgaacgggca
    17101 tgtattttat catcaacaag atgatgaagt ttcatcatat acatgtatcc gactgttact
    17161 ctgttatcaa aaggctgacc agttcttcca tcatatagaa tcgttttgcc atcttcagat
    17221 aatcctgcca tcttcagaca ttctataata tccatttcag aagccccgtc aaatactggt
    17281 gtagctattt gccatcctaa tgctcttgca gcataaccaa gatgaacttc taaaacctga
    17341 ccgatattca tacgtgatgg aactcctaac ggattaagaa ctatttgtaa tggtgtgcca
    17401 tctggaagga aaggcatgtc tgcttctggt aatacacggg atataacacc tttattacca
    17461 tgacggcctg ccatcttatc gcccacagat atctttctct tctgagctat aaatactctg
    17521 accacctggt taactcctgg acttaggtca tcacctgatt ctttagtaaa taattttaca
    17581 tcaacaataa tacctgactc accatgaggt actcttaacg atgtgtctct aacatctctg
    17641 gatttctcac cgaatattgc tcttaacagt ctttcttcag gagtaagttc agtttctcct
    17701 ttaggagtta cttttccaac aagaatatct cctgatctta cttctgctcc tacacggata
    17761 attcctcttt catcaagatc cttaagagct tcatctccga tatttggaat atctcttgta
    17821 atatcttcag gtccaagttt tgtatctctt gcttcaagat catattcttc tatatgaatt
    17881 gaagtaaata catcctccat gaccattttc tcacttataa gaatagcatc ctcgtagtta
    17941 tatccttccc atgtcataaa tccgataagt atgtttttac caagagaaat ttctccacca
    18001 tctgttgaag gaccatctgc tatcacgtca ccagctttaa caaattctcc ttttttaact
    18061 ataacttttt gatttataca tgttccctga ttgcttctct tatactttaa tatattgtag
    18121 ttgtcttttt gaccgtcttt tgttactaca acaactttat ctgcagaaac tttctcaaca
    18181 gtaccttctc tttttgcaag aacacatact cctgagtctg tagcaacttt atattccatt
    18241 ccagttgcaa ttatcggcgc ttctggttta attagaggaa ctgcctgtct ttgcatgttt
    18301 gatcccatca gcgctctgtt agcatcatca ttctctaaga aaggaatcat gcttgtagct
    18361 acagaaacca gctgtttggg tgaaacatcc atataatcta ctgtattgga atctctcatt
    18421 actttgttat ttctgtatct gcagcttact cgtggctgtg cgaaatgacc atcatctgta
    18481 agttcagagt tagcctgtgc aactatgtaa ttatcttctt catcagctgt cagataatca
    18541 ataacttcag taacaacatg agtttctttg ttaactttac gataaggcgt ctcaataaag
    18601 ccatattcat ttattcttgc atatgtgctt aatgaggcta taagtcctat gtttggtcct
    18661 tcaggagttt caacaggaca catccttccg taatgggaat aatgaacatc acgaacttcg
    18721 aagtttgctc tttctctgct taatccgcca ggtcctaatg ctgataatct tcttttgtgt
    18781 gtaatttctg caagagggtt gggctggtcc ataaactgag ccagctgact agaaccaaag
    18841 aactctttaa taacagccgt tactggacgg atattaatca aagcctgagg tgttactata
    18901 tctatatcct gaatagtcat cttttcacgg ataactcttt ccattcttgc aaaacctatt
    18961 ctgaactgat tctgcaataa ctcgccaact gatcttatcc ttctgtttcc aagatggtca
    19021 atatcatcaa cattatatag tccatagtct agtcctagaa tgtaactgat tgaagcgata
    19081 atatcatatt tagtgatatt ctttggacag agttcgtatt ttctcttttc aaacgcttct
    19141 ttaatatctt tttcattatc atatgtatcg agtatatctt ttaaaacttt ataactaact
    19201 ttatctgtaa gaccaagtgc tttgtaatca aaatcaacaa agtatgctgc attgactgtg
    19261 tcatttccta taaccacatg aacatggtta tcgcatttta cttttacaac attaattcca
    19321 gaattttcaa ttgcatttgc ctgctcttct gtaatcttgt catcttcgct tacaagtatc
    19381 tcacctgtgc tcttatcgat aatagtttca gcagaaatat ttcctactaa tcttggagct
    19441 atcgataatt ttttattgaa tttatatctt ccaaatctag ctaggtcgta tcttctagga
    19501 tcaaagaata agccttcaaa taagctttca gcactttctg ttgtaggagg ttcgcctggt
    19561 cttaatcttt tatagatttc aaacagtccg ccttctcttg tttttgtttc atcttttgca
    19621 tatgtggcat caagcttgta atcctcacca aatactgcac ttatttctgc atcagtagca
    19681 aatcctaatg ctcttaaaaa tacagtaaga ggaagctttc ttgttctgtc aattcttaca
    19741 tagagaattc catttgaatc agtttcatat tcaatccatg ctcctctatt tggtatcaag
    19801 gtactggaat acaggctctt gcctgttttg tcttttttct cagcgtaata tgtgcctgga
    19861 gatctgacca gctggctgac tataaccctt tcagcaccat ttattataaa tgtgccagta
    19921 tcggtcatta tcggaaaatc acccatgaat atttcttgtt ctttaacttc accggtatct
    19981 tttctaatca atctaacctt gacccttaaa ggagcctgat atgtcgcatc tctttcttta
    20041 cattcttgaa catcaagctt tgggctttct gcaattctgt aatctaagaa ttccagaata
    20101 agattatcag tatgatcgcg cacaggcgat acatccttaa atacttccct aagaccttcg
    20161 tcaaggaacc actgataaga ttttttctga acttcaataa gatttggcat ttctagaact
    20221 tctttgattt ttgagaaact cattctttct cttttcccgt attgaatagg atgtatcatt
    20281 aattagtcac ctcatgtcct tttctatctc atcctcttgg tatcggttac catttaagct
    20341 taggaaaacc ggtgccatat gttaccgtat gcacccagat atcatgaatt ttcatgtcga
    20401 caggcgcaaa aatacgcaga ttagcgcatt atcaattatt ctatatcaaa cgcttccgcc
    20461 tgtcaatagc ttatataata tttctttata ttgttggcta cctatttttt gtctttgcat
    20521 taattcaaat aagtgccttt tatcttctca attcgcccta ctagaaattt ctttagctga
    20581 ggtgacgcat catctacaag cttattaata cgctcacctt gattatttat ctcttccgat
    20641 aaaaaatctg ccattttctt atttgtctga aggtcataaa tccgaatatt cttaagagta
    20701 gactcaattt cgtgatctat cttctcaaga agagccatat ccgcttcatc aagcctcaac
    20761 tcttcgaata tatcatcttc aaaatttaaa aaattatcta catcagtttt tttcataaac
    20821 acctatatcc attgtattct tttccggctg ccttactatt gttgcaatat actcttcatt
    20881 ctccgctaca aatactgtat ctgatgtaac aagtatatct ttgaatcctg cagctataat
    20941 atcagcagta taagctgcaa aattagtttt tccgatatcc tctgcaataa cagtaatgct
    21001 gttagactct ccgataatct gtatgatctt aacatcagga ggaacaggca gcatagtaat
    21061 atatccctct ttaggccatg acagaggagt agtcagctca agaaccatcc tgttagtatc
    21121 attatctcta ttcattacaa tagagtattc atttaaatta cctgaaaaag cttcttttgt
    21181 ttcaactgct atatcagagt atccagtctg tttcagcagt tcaatatatt gcagaaatga
    21241 attttcatca acattatata tatatattct taaaactctg tcataaagtt tgtagtaatt
    21301 cacatctccg tcctgtattt tgggtaaaac atctgagcct gcatattcag taggccagtc
    21361 agcatctgat gaaatcacat gagttggtcc aggtgaattt gtttcatctg tcttgttatt
    21421 gcatgagcac aatgaaaaca ttagcactac agcaatcata atcattagag tcttttttac
    21481 cattctctcc tccgttaaat gctttttctg taatagttta tcatacaacc gtctgcaagt
    21541 acatctcttt cagtttctga taatccttcc atgtataaga ttacaacttt agctttgcta
    21601 tcttttgtca taacagtctg tttatcagaa gctatgtaag cttttatagt ttcttcacca
    21661 ttaagtatgg ctttccttat acctggtata tagacgtaat cattcgtatt aagttccaat
    21721 tcctctttta taataaaagg aatcattccc cagttaatga ggttactacg gtacctttta
    21781 gtagcatact catatgctat attagcaaat cctttaagaa ctcgctgaca tgatgctgct
    21841 tgttctcttg ctgagccgtc acctggcttt ttcgcaaaaa taaggtgtcc gtctacttcc
    21901 tcttctttaa gagccatttt tgtattcatt acataatctg gagcttttct tgataatgtg
    21961 aattccgcta aagccatagg gttagacctg tatgatgatg tctctccaga tggtataagc
    22021 tcatcagttg tagtaacttc atcttttaat actgcagcaa ccttaagtaa aagattctca
    22081 ggacattttg gtatttcagg ccatggtttt atattcgggc catatatgag gtcttgagaa
    22141 taatcaggct tattataacc atagtacacg cgtctatcgt aaatgctttt attgaattca
    22201 tattccggaa tagatttttc ataatcaata tcagtacctg gtgttatgta acctttattt
    22261 agcgcagtag cggctatgct ccttgcatcc attaaagcta cgtatgctga ctgcccgttc
    22321 ttaggtattg atccttcccg gttttcaaaa tttcttgttg tatggcgaac agataatgtg
    22381 ttatttgcag gcacatctcc tgctccaaaa caaggaccgc agaatgcagt ctttattact
    22441 gcacctgctt ccataagctt attcatgcac cctatcttgt taagagcata tgcaacaggc
    22501 tggctttgcg gatacacaga caacgagaaa cgatcgcttc cagtatctcc tttttccagt
    22561 atttcagaag cttcaattat gttttcatat gtcccaccgg cacatcctgc aataatcccc
    22621 tgacctgaga agaagctctt gttctttgta tcagcaaatt cattaagaga atctagcttc
    22681 aagctatgaa gtatatccaa actgttatct aaaaattctc ttatcgtata tgcgtttgaa
    22741 ggatgataag gcagagcgat catcggttcg atttctgaca agtcaataac cagggcacag
    22801 ttgtaataag ctttatcagc tggctgcaat tttttatatg catcttttct cccgtgtatt
    22861 tcgtagtact tccttgtcat ttcatcagtc acccatatag atgagagaca agctgtttcc
    22921 gtagtcatta catcaattcc cattctgtca tccattgaaa ggttttttat gccaggtcct
    22981 gcaaattcca gtattttgtt cgttacaagc gctttcttaa ataatttccc tatcatatat
    23041 attgcaacat catggggtcc aactcctttt ttgagctggc cttttaaata gatgagacag
    23101 acttctggat acttaatgct gtaagttttt cctaaaagct gtttagccag ttctccgcca
    23161 ccttctccaa ttgcaattgt tcccaaagcc ccgtacctgg tatggctgtc cgaacctaat
    23221 atcatatgcc cacactgtgc catcatttct ctcatgtact gatgtataac cgccatatga
    23281 gctggaacat atattccccc atatcttttt gctgcagaaa gtccgaaaag atgatcatct
    23341 tcattaattg tccctccaac tgcgcataaa gagttgtgac agtttgtcag aacatatgga
    23401 atattaaacc tttgaagatt acatgctgct gcagtttgca atatactgac ataagttata
    23461 tcatggcttg caatagcatc aaacctcatt tcaagattcc ctttcttatc tgataaatca
    23521 tgtttttcaa gtatagagtg agctatcgct cctttttttg cttcatttat aacctctgtt
    23581 gaaagcatgc tagcttttat tatcttgtct tccattaaaa ctactggttc atttataaac
    23641 atgtaaaaat ttgttccttt catatgttat actctattat atataaatcg gaaaggtatt
    23701 gaaagagaag aataggtata taatgaaata caaacacata atctgggact ttgatggaac
    23761 gctttttgac acatacggag aaatatcatt agttataact gaagtactga agaaaaagaa
    23821 cattgtggaa gacaataatt atatttcgaa aaatcttcac aaatcactca ctcatacatt
    23881 gaaattactg tcaaaaaaac atttacttga ttttgataaa cttgtcagcg aattcatgtt
    23941 tgctgaagaa aaaatgaatg ttaaaaaagc aatgcctttt aacggagtga aaaaaattat
    24001 tgaatcaatt gaaggatata actttatagt taccaacaga ggcgaatcag tattcagatt
    24061 tttagaagac aaggattatc taagattctt tgaagaaata ctatacaggg attccggact
    24121 ggaattaaaa ccaagcagtg cgactgtaga ttatcttgtc agtaaatata accttgacaa
    24181 gagtgaaaca ctgtttatcg gtgatcgtga tattgatatt gaatgtgcaa acaattcaaa
    24241 catagattcc tgttattatg attctcataa catcatcaat actgctcctt gcacttatta
    24301 tgttaaagat tacaagacac tcattgagct tctagacatt agtaaataaa ttagttaaca
    24361 ctataatcga caatagaata acataaactg caagagccgg aagaaatgca ctcatggcnn
    24421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    24481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnta atcgacaata gaataacata
    24541 aactgcaaga gccggaagaa atgcactcat ggcattagca tccatttttg cttgttttga
    24601 tatatttaat ttggtatgtt cagtgttctt tcttaacttc tttattaaaa ttataattaa
    24661 tggcaatact accagtgtaa gcgcacccca tactataaaa gagataccat attctgataa
    24721 tccataaaat tcagaaacat cttcagaaga tactgttgaa ggtagaactg aagcaacata
    24781 agttatggtt atcgcaatca ggttattaac aaaatgacct ataaatgaag atataactga
    24841 acctgttcga taaagaaaat agcaaagcag tattgaaagc gcgaaggttg aaaaaagttt
    24901 ttcaggaatc atatgcgcaa gcgaaaagaa aagcgatacg aatataaagc atgaagtttt
    24961 tccaagtctc tctagtccgg acataaatgc agttctgaag gctatctctt cacatactgc
    25021 tggaacaagg gctaaaacaa gtatgctttt tatataatca gggaaattat ctatcataat
    25081 agagtcttcg agtcccgctg taagtcctat cgtatgctct gtaatccaca tgttgaaagt
    25141 agctaaaagc atggatacag gtataaataa tgcgattatt gcactaattg taagagtatt
    25201 ttttatacta acccttctta tgtgtatatt gtcttttgct cttttggtaa taagaagaaa
    25261 tgcaagtaca ggtattgcca gaccaagtat ctcactgata gcctggtaca ggtacagatt
    25321 gtccattaaa aagaacattc ctacaacctg aacagcaaga aaaattatca taacaactgc
    25381 tattaataaa aaagattgat gtatttgaag tttgcggtct tctctaagtt ccattattct
    25441 ccgttaagcc actttatctg gtcctttgat aacataacat ccttgacttt catgctgtct
    25501 tccagctgag ataaattcct aggacctata atagcggatg tctgaacctt gttatttgtc
    25561 agataggcaa gtgcgataca tgtgggagag acactatact catctgcaag ttttttggct
    25621 tttcttgctc tttcaatatt atcatcagac ataaatctga ctatatcact ttttttcata
    25681 ttgccgttgt gaattttcgt aaaaaaccct tttgcctgtg aagtatatgc cattaacggc
    25741 atcttactct tctcatacca ttcatattcg gctttattta tgcagactat tgttgaatca
    25801 tcaaattttt caggagttga ttcggctaaa gaaaagtaac actgtgataa aacaagagga
    25861 ctcatatttt cttttacaat aaactcattt gctttttcaa ttctttctgg cttccagtta
    25921 cttactccta caaatcgcgt tttaccctgt ttaacgaaat tatcaagtat gggtattatg
    25981 tcatcaactg gttttgattc atcatctctg tgcaacaaaa acacatcaac acaatcaata
    26041 tccattgtct taagacttgt atccaaatca tattgcaaat cttcttttgt tatcctgtta
    26101 acaacatcct tctttatttt caaattttta ggaaaaccgc ccttagtaat tatgactgtt
    26161 tcatctctgt tgttacgcga cttcatccat tgtccaagca tgtactcaga tatgtctttc
    26221 ccatctggaa gccattcgtt atatgaacgg gcagtatcaa aggttctgaa attttcaaaa
    26281 aatctatcca ttaaaacaaa ataactctct ttatcattgt aatcagaaaa acaacctgtt
    26341 ccaaaggcaa gcttgtctaa actcaaactg tcattgttat ttttaattgt aatctttatc
    26401 atttaaaata ctcctttgcc aattgagtaa tcttaggcag actggatttt attgtttcat
    26461 aagatacaca aaaacacatt cttatatatc ctggcatacc gaatcccctg ccaggcacaa
    26521 tcaatatatt gaaccctttt gctttttcaa caaattccat ctcatcctca actggtgact
    26581 taacgaataa ataaaatgcc ccttgtggtt tttttatact gaatccgcat tcaatcatat
    26641 gattatatag aagatctctt cttcttttat aatcttctac atcaactttc gcttcgcatg
    26701 actgctgtat tactctttgt attagggcag gagcatttac ataacctaat gtccttgtat
    26761 tgaaaataca tatattcaga agtaattcag cgttcggtat tttatggtct acagctatgt
    26821 atcctattct ctctccagga agtgcaagcg acttgctata tgaatttata atgatagctt
    26881 tatcaaaaca tttaaatacg caaggaactg tagcatcatc aaaaactatc ttggcataag
    26941 gttcatcact taaaacaaaa atatctgtgt tatatttttt ctccacctgc cttatagctt
    27001 catttagttt caataatact tccttagggt atatgactcc cgaaggatta ttgggggtgt
    27061 taataataac tgcttttgtc cttgatgttg ctgttctcat aaaagagtca atatcaggca
    27121 tgaaagattc atcgcaatct gctactttaa gatttccctg agcatgcatt acataaaatc
    27181 catattctgc aaaatatggt ttgaatacta ttacttcatc atcaggattc aaaatagact
    27241 ttaaagtaac aaaaagtcct gcagctgctc ctgcagacat aataatattc ttataaggga
    27301 tatcgtagtc gtaatcattc ttaatgcttt ttgcaactgc ttttcttgtc tgttcatatc
    27361 cggcattgct catatactta tgcatgcctt cttccttgct catagctata tctagaagac
    27421 tttgcttgac ttcaacagga ggttccattt caggattccc tatagaaaaa tcataaacat
    27481 ttttctcacc atatattgct tttaatctgt tgccttcttc aaacattgca cgtatgaagg
    27541 atgatttact taaattttca gttatttttt tattaaacat acatatctcc tattccgtaa
    27601 tattacaata aggatgactt ttttgcaaac tgaacgctta gctttttcta agcttttcaa
    27661 ctgcttcttt atctgcttta acataagctg aatagtaatt aaaataattc acatatccgg
    27721 gttttgaacc ttttggcttt ttaacatttt tagcaaatgt ataatccaca gccacttgtc
    27781 ctgaatcttt accttctgaa tgaaatgcag ctaccatagc agcttcagtt aacgtcttgt
    27841 ctggcacaaa ctgttcagtt tgctccttgt ttttaactat tacatgagac ccatgcatat
    27901 ttttaatatg cagccagata tcataagctt ccgaaaaacc gaatgtaagt ctgtcattat
    27961 cagtattatt cctgcccgca tatatctcat acccctcaga agaaatatac tctataggcc
    28021 ggaataaagc agtttctgtt tcaatttttt tgtttgattt ttttcgaagc tgtttttctt
    28081 tcatgtaacc ctgttgaatc atctcttttc taatgtcttc aatatcctgt tttttttcac
    28141 atgataaaag tgcatgcatc actgactgca gataatcctt ctcttttttc atttctttaa
    28201 ttgcttttat actataatcg tatgcagcca tacctttttt atacttctta tagtatgact
    28261 gagcattttt caatgcatct tttgaaggat ccataggaat atcaatcatt tcatttgtat
    28321 aatagtccag caactgcaca ttctcagctc ttttcttaag catgggaaga tttgccataa
    28381 taagctcgcc atacaacttg tattgtttca ctttgtcagc atctgataaa ctgttttggt
    28441 atatgttcag tttcttttca acttttccaa gatgagctgt aaggattttt ttaatagtat
    28501 cagatttttg tgaaagctcg tatactcttg acttttcacc tatatactgt gtgaatacat
    28561 ctaaaaacga gtcgaatctt tttactttga tactttgaat attttcatac attgaaaggt
    28621 ttaaacagta aaaatctttc atgtttttct tgttatcgtg atatattgcc acaggttcat
    28681 aactgtaact ttctgatata tcagcaattt gtgaaagagc agctgataaa tcttcttttt
    28741 ctttatcagt cagatattta gcagatttca tatcatcaac tcctgctctg taacatactt
    28801 ccttaccaaa tactggagca gtccccatta ctgttttaaa tattgcatca gagacactga
    28861 attcagaata aatatcaaga tcttcgattg ttacattatt aataagtttt ttgttctgca
    28921 aaggtggaac agcatactca cttgcaggca ttatccttct tactgaagac ataaattcat
    28981 ccacatgcct aagagcatcg agaatcttat tatgcgaatt tacaacaata agattacaat
    29041 ttcttcctgt tagttcgact atcatagttt ttttagacgt ttcttccagt tcatctgtat
    29101 gattaatatc aaaaagtatt actctttcat aatcttttgt atatactctt tcaatccatc
    29161 ctccgcctat atgttttctt aaaaacatac aaaaactata tggggtcttt ggattatcca
    29221 ttttatcata tctcaaatat atacctggac tgcttggttc cattgatatt gtcagataat
    29281 gtgatttttt atctccatat atactcaata caacttcatt atttctaggc ataaatatat
    29341 tctgtatctt ggaataccta caaagctggt ttaattcatt agcaaggcat cttactgcca
    29401 ttccgtctag cggcataact tctccattcc agcggcaaca accgcaattg tcgtaaatac
    29461 gaatactaaa tcgtagaaac taatactaaa tcgtggaaac gattgttaaa tcgttttata
    29521 tatattatca tatatccgta cttttatact atttagaagt cggaaagagg aatctattgg
    29581 aacttttatc acccgcaggc aatatgcaaa gccttaaagc agctgtgcaa aacggagcag
    29641 acgctgttta tcttggactg aaaactttca gtgcacgtgc aaatgccgac aactttacat
    29701 ttgaagaact taaagaagca ttaagctatt gtcacttaag ggatgttctt gtttatgtaa
    29761 caatcaatac tttaatagat gactcaaaca tgtcatttgc tttagacaat gcatacaaag
    29821 catatacata tggtgttgat gccgttatag ttcaggattt aggattagca gcacaaataa
    29881 gaaaaaacat accatctctt agacttcacg catcaacaca aatgagtgtc ataaatccca
    29941 aagaagcaat acttttaaaa aatatgggat ttaaaagaat aatagcggca agagaagcga
    30001 cgattgagca aattaaggct ttgactgaaa ctggtataga aattgaagta tttggccatg
    30061 gttctttgtg cgtaagttat tccggacaat gtgaattaag tttctttaat gggggaaaat
    30121 ccggcaacaa aggaggttgt gcccaaccat gcagactgcc atacacctta gaaaaggaag
    30181 ataaagtgtt agcttttaaa aatcttctat ccacaaaaga tatagcagta atggatcaca
    30241 tcaagcaaat tgaaaatgca aatgtagtat cgttaaaaat tgaaggtcgc atgaagagcc
    30301 ctgaatatgt agctataatt acaagaacat acaaaaaagc aatatccggt accttaactc
    30361 aaaaagacag acaagacgct atgatggtat ttaacagaga gggtttttca gaaggataca
    30421 tgttctctaa acctggaaaa gaaatgatgg cttacaattt ttctggtaat acaggtataa
    30481 aaatcggcac tgtaatcggg gtaaataaag aaaaaggcct tatacagata gaatccgaca
    30541 aggaacttgt aaatggagac ggaataagtt tcccaaagaa cgatttaggg atgtatgtaa
    30601 atattataag aaaaaatggt aataagtatg gtcttatatg cagaaatgga gtacctgata
    30661 atttcgagca tgtctatctc acatacgata aacaactaat ggaagaagcg caaaaatcct
    30721 attcagatga tttttacaga aaaacagcca taaagggtgt atttcaggca aaacatggag
    30781 aaaaagcaaa attcacagtt actgataacc gctcaaacag ttttactgca tattctgaca
    30841 tagctgtaca agaggcaaat agtcccgatg cgaatcagaa tgttctaaag aatttatata
    30901 aaaccggaaa tactgtattt gaattcgaag atatatacat taaaactgat tttgatgttt
    30961 ttcttccggc aaaaactgta aatgctttaa gaagagatgc gctagaagga ttgacagaaa
    31021 taaggaaagc aatatctgtt gttcctgttg ataaagtttt tccaaagcat cttaatataa
    31081 accgctcaat aaacagaact gacaaaaagc ctggagtatc acttttcttt ttcgaagaca
    31141 gacctgattt tgatataaaa gacatttttg ccgatagaat ttatatgccc gaaagtatgt
    31201 ataaaagata ttcatatgat aaccgggcct atatgtataa accaataggt tcaatgctgg
    31261 atgaaaaaac cattttgcca aaaagagtta tttcatcctc aatgggaatc ttagagtatg
    31321 attgtgataa ggtaatggat tttggtataa attgcttgaa tacttatact gcagattttc
    31381 ttgcaggtta caacaatgta aaagcggtat gtctttcaca tgaactgcca attgaagaca
    31441 tatcttcttt tacaggaatg aattctaata ttagttttga atatatagca tatggtcgtg
    31501 tagctgtaat gaaaactaag tactgcataa aaggagcgtt taatcagatt cctgaatgca
    31561 ttaatcaaga cttgcacctt ctagacagac aaaagaaaag aatagatatc gttacttatt
    31621 gcgattgtca tacttcatat cttttaggag ctttcacaat aaataatata aatcgcattg
    31681 atgaaattag aaaatccgga gctgatacgc tacggattaa tgtatatagg gaaacaaaag
    31741 aagaaataaa agaaattatc gacaaacttt tataatcaga gttccttaag ctgtcttatg
    31801 gtttctttta tatcgtctga tttaaaaata gcagagcctg caaccaaaac atttgcacct
    31861 gcctttttaa tttcctgaca gttgtcgagt gttacgcctc catctacctg aatatcaaga
    31921 tttaatccca tagaatcagc cattttcctc agttctttaa ttttctgagt actgtatgga
    31981 atatatgatt gtccgccgaa acctggattt acactcatta taagaaccat atccagcaga
    32041 tgaaggacat ttttaagtac tgatatatct gttgccggat tcaatgcaac agctgctttc
    32101 tttccatttt cctttatatg ccttattgtc ctgtctaaat ggatacatgc ctcctgctga
    32161 acacatatta tgtcggcacc tgctttagca aaatcatcaa tatatctgtc gggatcagtt
    32221 atcattaaat gaacatctaa tacaagatct gtaatattac gtaaagattc cacgacaggt
    32281 atgcctactg aaatgttcgg tacaaaatgg ccgtccataa catcaatatg gacatattgt
    32341 gctccacctt cttctatttt tctaacttcg tcttcaagtc ttgcaaaatt actggcaaga
    32401 agtgatggtg agattattat catcagtatc ccctcctgtt tttcaaagct tctttagaca
    32461 tattatatat cattttatac ctgttgtatc gtccgattgg tataagattc tcttcaaccg
    32521 ctttaattac tgcacaacct ttttcattta tgtgtttaca atcattgaac ttgcatacta
    32581 atttgtattt atcaaactcg ttatacatat ctttaattgc ctctggttca agcaaacctg
    32641 gatcaaattt ggaaaacccc ggtgtatcaa caatatatcc ttccatgtat ggtagaagct
    32701 gacaatgccg tgtggtattc tttcctcttt caatctttcg gcttaaaatg ccagtctgca
    32761 tgctttttgt ttttgtaagt gcatttatta gtgtcgattt tcctgctccg gactgacctg
    32821 caaaaacagt gattccagag gaaataactt ttgaaaacct atctagatta tccacatgaa
    32881 gagcatcagt cacaattatc tcacaaccgc acttctcata ttccttcatt acaagtctta
    32941 atttgtcatc atctgcaaga tctgatttat taatgcataa aatggactgg attttatgtg
    33001 ctctgcttgc aagtattaga agatcaatta agaattcatc cagctttgga ttatttattg
    33061 aaaaaacaat aactgaagta tcaacatttg ctactggagg tcgtataagg ctgttctttc
    33121 tttcttttat cttctgtata tagccttctt tatcagaaat atctgttatt tcaaattctg
    33181 ctatatcacc gcaaagaggc ttaaaaccat cttttctgaa caagcctctt ggtttgcaga
    33241 tatattcatc tttttctgtt tttactgtgt attgcccgct aacagatttt gtaactaaac
    33301 ctatctccat ttatggttca actggcgcat caggcaatcc tggttcgccc tcaggagtat
    33361 ctccaggttg atctgatgga ctgggtgtcg gtacataata gtcgaaatac ttataaacta
    33421 ttggatcttt gtaaggaact gaacctataa atactgttaa ttttgtttct ccttttgcag
    33481 gtatcctgat atctctttca tatggaagtt tagacagatc tgcaacattc acagatgtct
    33541 gagatgatcc agattgatca gaatgctcca acttaaatct taaggtaagt ggaaattgca
    33601 tatttgatat ctctgcaatt gtagggtcaa aaacatattt agcatatatg tcttcatata
    33661 ttacaccttc ggtatcaaca gcaagtttaa catcaattgt actgtgctcg tatatacctg
    33721 aaccagcttc cggatattga tatgttatta taggtatctt acctggaatt tcttcttctg
    33781 acattatatt cattacaaga ttgaactgtg agagtcttgc agcagcttgt tcttttgtta
    33841 agcccattag atcaggtaca gtagttttct catactgaga tccaatggat acatatattc
    33901 tgattgtttc cccatatttt acctgagtat tttctaaagg tgacactgaa attactattc
    33961 cagcatcaat atcagtactg tattgttcta ctttctctgg tacaagtccg atattaataa
    34021 tttctttttc gacaacacgg tagtctttac cttcgcattc aggtacagat acataattgc
    34081 ttcctatact tactgtaaac gttatagtat ttgtcgcacc ttctttaaat attcttccct
    34141 gctcaacatc ctggtccatt attatacctg caggagtttc atcagaatat gcttctttca
    34201 ttagtacgat tattccaaaa tcttcttcta attttttctt gatctcatca tattcatatg
    34261 ttacataatt caatacttca tattctttta tttctggcac tacaacctta gcaagttcat
    34321 ctatcgcata cacgacaaaa acagtagcta aagataaaac catcaatatt gccacaagag
    34381 tagtcagaaa atatttcttt tcttttttct ttcttcctct ggcataaacc aatggaacgt
    34441 tatactctaa atcgaaattt atctgtttct gcccttttat atttcctgta ttcctcttta
    34501 cccagccagt attactcttt acaatcccct gttcttcatc aagaaataaa tcatcttcag
    34561 ttactgaagg aattgccata gtatcttctt gatttactac ttctccatca ataaaatcta
    34621 aagttaaatc agttcttcca tttccaatat tctccagatt cttcaaatca atcatcgcgt
    34681 ccgctgctgt ttcatatctg tcagaaggct tcttctgaat tgatttcaaa attattctgt
    34741 taagttcctc aggaatatcc tcccttatct caattggagg agtcggatta tcctgaaggt
    34801 gcttcataac tattgcaata ggagtgtcag catcaaatgg tacttttcct gtcagcattt
    34861 cgtacataac aatacctgta gaatacagat cagtactgct gcttaaaaat cctccttttg
    34921 cctgttcagg agatatatag tgaactgaac caaaggtttt gttatctgtt gtcagtgttt
    34981 gtgcattaat tgcttttgct attccaaaat ctgttacctt tatcttgcca ttcttcgata
    35041 tcagtatatt ctgtggcttt atatctctgt ggataattcc attagtatgt gcatgttcca
    35101 atgcagcaaa tatctgaagt gaaatatcaa tggtttcttt aatttctaaa ggcccgattg
    35161 attccatata acttgataat gtagtgcctt caacgcattc cattacaata tagttaaggt
    35221 tattatcata cccgacatca taaacgttga cgatattttc gtgtgataat tttgctactg
    35281 atagagcttc atctttaaaa cgttttatga aatccttatc ttgagcatat tcatctttaa
    35341 gtgtcttaac tgctactgta cggttaaggg ctttgtcctt cgctttataa acataggcca
    35401 taccgccagt gcccatcctg ctgagaagtt cgtatctgtt attcagtata acagggtcca
    35461 tttctattcc tccgccttct tttcggtttt gataagtacg atagttatgt tatctgttcc
    35521 accattttca ttagcttttt cgccaagatt ccttactttc gtatctaaat cagtatcctg
    35581 attaattata ttcaatatat tattatcgtc aagcatatta gtaagcccgt cagaacataa
    35641 cagcatataa ccgcaaaaat tatattgcat ttcgaatatg tcgggttctg gattatccgt
    35701 tattccaagt gctttagtta taacattctt attaggatga tttttgaact catcaaacgt
    35761 aatatctccc ttatccagta actgctgcac ataggaatgg tccttagtaa ccattttaat
    35821 gttatcgcca cttatagtat agattctaga atcccctata tgaattatat gaattttgtc
    35881 atcctttata tagatagcat ctaaagtggt tcccatccca gaaagaacat tgtgtttttt
    35941 gctgaactca agtatttctt tatttgataa cttgcataca ttatataagt aatctttaat
    36001 gttaatacaa ttcttaaaac gtctgtccct tatttttttc tcaaaagtat ctattgccgt
    36061 tttacttgct atctcaccaa aaagatgtcc gcccatcccg tctgctacag ctgcagcaaa
    36121 ttcgttatgc tcctcaaatg gaataactat gtgataatcc tcgttatttt ccctaacttt
    36181 gccgatatca gtatatgaag cgtaattaat tatcattctg tctcctcagc tgtccacacg
    36241 cggcatcaat atcccttcct aattctcttc ttactgtaac agggatgccg ttttctttta
    36301 gtatagcaca aaatcctgat agattcttgc tgttttgatg agctgatttt ttatcaattg
    36361 gattgactgg aataacattt acataacaca taacattctt taataatgaa cataaacgca
    36421 tagcatgctc atcagtatca ttctttcctg atattaaagc atattcaaaa gatactcttc
    36481 tatttgtttt ttcaaaatag tattcacaag cctttataac ctgctcaata ttatactttt
    36541 tattaatagg cataatagct gttctttctt tgtctgtaac ctcatgcaaa gaaactgaaa
    36601 ggtttacctg cagacccaaa tcagcaaatt cataaatttt aggaactatg ccacatgttg
    36661 atacggttat tcgtcttcct cctagattca atccctcttt catgttaata atcttcagaa
    36721 aattaacaag attatcgtaa ttatcaaaag gttctcctat gcccataaca actgttcttg
    36781 aaatattctt attgctgtct gatgcagctt taagtatctg tgctgttatc tcatatcctt
    36841 caagattacg gctgaacggt actttggatg atgcgcagaa aacacatccc atcttgcatc
    36901 cagactggct ggacacacag attgaagtac cataatcata atccattaca acgctttcca
    36961 ctgttgcacc atcagagtat ctgaacaaat attttattgt tccgtcttgt gaaacaagtt
    37021 ttttttcaat ttcaggtaat tccagtgtga aattttctgt taataaagct atatcttctt
    37081 tacttatatt actcattaat gaaaaatcaa aaattttttt tgagtatacc cattcaagaa
    37141 cctgtattgc acgatatctt ttcaggccgt actgttgaaa aaggtctttg agttcgttat
    37201 gagtatatga aagtatatgc gttttttgac tattcatgag ccaaattata tattttactc
    37261 cttaaaaaat caacagactt gaaaaaatgc tattttctta ttagtctcgc agcatagaaa
    37321 ccctgatcga aaaaatcttc ttcaaatcca tattcattgt tttcacttaa aaaaccttca
    37381 actatatcct gattctcttg tcggttaatt gtacatgttc catatactag atacccgtct
    37441 ttttttacat atcgtgagca tgtctttatt aaagctttct gtgttttagt tagtgaatca
    37501 gatgttttat acttcaactt aatttcagga cgcttgccag caacaccaag accggaacat
    37561 ggagcatcta taagaaccct gtcaaatgca ttttcatatt caggaaagta ttcagtcata
    37621 tcttttacaa aacagcttat gttagtaaat ccacatcttt ctatattttc tacagttttg
    37681 gatactctac tctgtacaat gtcacaagct gttatgtttc ctgtattttc cattgtattt
    37741 gcaagataaa tagttttgcc gccgggtgaa gcgcaggcat caagtacagt catattattc
    37801 tgaacattaa gaagctttgc aatctgtact gctaactcat cttgaacgat tattgctcct
    37861 tttgaatatt catttgatga tgatggtgat gaattgcctt ttaccaatat gctggtatta
    37921 tcagtaatct ctatatcaaa tccctgtttt tgcatattat aaattacttc atccttatta
    37981 tgaaaatgtg tgttaataga gatatatgta tcttgtggac tattgaattt tttaagcatt
    38041 tctatgcatt gctcttctga aagcaatgat ctcatagcct tatatgtttc tttagtacaa
    38101 gagtataata tatgcggttc aatattacta tcttcgactt tattttttcg tataaaattt
    38161 cttagtactg cattaacaaa acctgcagaa ttcttgctaa cgactttttt agccagattg
    38221 catgattcat caacaattgc atattgtgga atcttatcca taaacacaaa ctgatatatt
    38281 cctatcttta aaatacaaag cagtaaagga ctggtctttt taatgttttt tatatacggt
    38341 ttaaggatat gctcaagata aatgctattt cttagtactc ccatagatat attcatacat
    38401 aatcgcctgt cttctacaga caattttgct ttattcatat aactttctaa aaccatattg
    38461 gaatatttat ctttatatat aacatcatgg actatattta atgcagtatg tctagaatcc
    38521 atcaacatcc acctaaagta attccgcaag taagattatg accgcactgg cataactgca
    38581 ttctttttcg atcttcaaac tgcagttccg ttatatacag gattccagat tcgcatttga
    38641 ctgccatctt gtctttctca atatttaata tagtcccatt tgctatggaa ctgtcattaa
    38701 catcatctga gtattcagct tttgttatct tcatagtttt tccacagtat gatgtatatg
    38761 ttcctggcca ggggttaaat gcccttataa cattgaaaag tcttaaagca tcttgattaa
    38821 aatctaactt tccttcttcc tttttaatca taggagcata tgttgctaaa gaatcatctt
    38881 gttttactgg tactatcttg tcttgttcat aaagcttaat tgtatctata agaagatttg
    38941 aacccataat acttaacctg tcatgtaacg agcaaatatc atcctcttta gtaatattcg
    39001 tttctttttt taatagcata tccccagtgt ccattccgac atctgtaagc atagttgtaa
    39061 cacctgttat ttcttctcct tcaataatgg ctcgttgcat tggagatgcg cctctgaatt
    39121 taggaagtaa agatgcatga acattgattg ttccatactt tggtatttcc aatatgcttt
    39181 ttggcagtat ctgtccaaat gcacatgtca ctgcaaggtc gcaccctatt tcagatattc
    39241 tttgcggcca attatctttt ctaatctttt ccggttggag aactggtata ttatttttta
    39301 ttgcagtttt ctttatattt gaaaactcta taacattatg ccttcctttt ggcctgtcag
    39361 gctgggttat aactgcatta atgttatagc ctgattcaat cagagcttct aaacatggta
    39421 aagcgaagtc tggtgttccc atgaatatta tcttacttat cttcttcaat ttcttcttcg
    39481 tttccctcta attctgacgg gtctatctct tctataacta gatctctgaa caaaatacca
    39541 tccatatgat ctatttcatg actaaaacaa tgtgcggtat actcctctgc ttcaacaact
    39601 tgtttgtttc catatctatc ctgatattca attttaactt ttaatggacg ttttactgta
    39661 ccataatatt caggaatact gaggcaacct tcaatatcta tattttcacc ttcttgttca
    39721 actataatag gattaataat ctcatacttt ttttcatcca tctcaatcac agcaactcgt
    39781 ctcaaaaccc caatctggac tgccgacaaa cctaatccag gtgctgctac catagtctca
    39841 tacatgtcct caagaagagt atgaagcctg tcatcaaatg atgtaacttc tctagatctt
    39901 ttccttaata tgtcatcgcc ttcaattcta atttttctta tagccatttc taatccgtcc
    39961 tttatcatta ctattttata ctactgttac attttaggca ataaaaaagg acaccctttc
    40021 ggatatcccc tttaataatt tttgtgttgc tattcagcaa atatagtttt ttctgattct
    40081 ttgtcgaata tgtgtattct tattgtatca attgctattt ttattgttga gcctggtctt
    40141 gcttttgtag aagggctgac tcttgcaata atattgctct ttgcaaagct aaagtataac
    40201 agagcttctg gtcccatcat ttcaacaacg tcaactttta cttctattgt tgtatctgtc
    40261 atctgatcaa ggtatacctg ttcatcacgg aaatgctctg gtcttattcc taagacaact
    40321 tcttttccat catatccttt accaacaatg ctctttgctc ttttttcaga gaacttaatt
    40381 tttgttccag ctaaattaat ccatacatca ttgccttttt cttcaataac agcattgata
    40441 aaattcattt gcggagtacc gatgaatcct gcaacataca tgtttgatgg gttatcatac
    40501 attatctgag gaggatctac ctgctgaatg aatcctgctc tcataacaac gattctgctt
    40561 cccattgtca tagcttctac ctggtcatga gtaacatata tgaatgtggt tttgagtttc
    40621 tgatgaagtt ttgttaattc aacacgcatc tgagctctca atttagcatc aaggtttgat
    40681 aacggctcgt ccataaggaa aacctttggt tcacggacta tagcacgtcc aagagcaact
    40741 ctctgtcttt gtccaccgga aagagctttt ggttttctat caagtaaatg ctcgatttcc
    40801 aaaattttag ctgctgaatg tactctctgt ctgatttctg cttttggcat ctttcttaat
    40861 ttaagaccaa atgccatgtt atcaaaaata gtcatatgag gatataaagc gtaattctgg
    40921 aaaaccattg ctatatctct atctttagga tgtacctcgt taattaatct atctccgata
    40981 taaatctcac cagcagtaat atcttcaaga cctgcaatca ttctgagtgt tgttgttttt
    41041 ccacatcctg aaggtccaag caataccaga aattctctat cttcgattac aagattgaag
    41101 tctgaaacag ctgtaatacc accacggttt ggctcatact ttttatatac gtttctaagt
    41161 gtaacttttg ccatatctga ttcctcctat acatttcttt gtttattcta tcacttaacg
    41221 ggtaagaata taagatggta tatctacaaa aagaaacata cctttttgta tattttgcat
    41281 aaaagaagtg ttatttgtgt agaataggtg aaagacgctt atacaaaagg aatgtaatca
    41341 atccgctcaa gataccttta attatattaa aaggtacatt tgctagaagt ataagatccc
    41401 atagagaatc tatattagag tttaatttag tccccattgc aacaattgtc tcaattggca
    41461 ttccaaaagc acttgcataa actggtatga ggatataata atttaagatc gctccaacta
    41521 acgtcatagc gaatatacct acaagtattc cgactattgc attctttatt cttttattct
    41581 tgttataaat catcgcagct ggaacaatta aacagcatcc cactatgaaa ttcgctaatt
    41641 cccctacccc tgcagtagct gtaccggtaa taacaaaatt aattagtatc ttgattgctt
    41701 caattattac tcctgcaaca ggccccattg agaaagctcc taaaagaact gccacctcgc
    41761 ttaaatctac ttcataaaaa ctaggcgcaa aaggcagagg tatctccagt aacataagca
    41821 acccagctac tgctcctaat atacccatct ttgccatatt ttttgtagtc ataagtttta
    41881 gtttacactc tgtcattttt acccctatcg tatttctcat ggtgaagtat aacatatata
    41941 tagttcaaat ttaagtcttt atacattatt ttcttgaaac tgtttatatt gaatttcata
    42001 catgtggtta ctattattaa aaattaatat tacacataat gctatccaat ctattcataa
    42061 atcattatta agcatacttc acagaatata tgttaaaata taggtatgct aaatgttaag
    42121 agtaaggaga aagcaaaatg aaactaggtg tatttacagt cgtattaggt tctatgaact
    42181 tagaagatac aatccgatat ctaaaggatt taggcgtgtc tgctatagaa ttaggaacag
    42241 gaggatatcc tggcactgct catgtcaatg caaaagagat ctttgacaaa cctgatgaaa
    42301 taaaaaagat aagagaggta gttgacaaat atgatatgca gataagtgct ttaagctgtc
    42361 acggaaatcc agttcatccc aataagaaaa tcgcgcagga ttttcatgat gattttgaaa
    42421 aaacaatact tgttgctgaa aagatgggta tagatactgt agtaacattc tcaggatgtc
    42481 ctggtgatta tgagaaagct aaatatccga actgggtaac atgcccctgg ccagatgact
    42541 ttttaaagat attagaatat cagtggaatg acgtattaat tccttattgg tcaaaacaag
    42601 ttgaattcgc aaaaaaacat catgtaacga aaatcgccct ggagatgcat cccggtttct
    42661 gcgtatacaa tccggaaacg ctactaaaac ttcaaagagc tgtaggtcct gtaataggag
    42721 caaactttga tccatcacat cttttctggc agggaattga tgttattaaa gcaatcagag
    42781 aattaaaagg aagcatttat catttccatg caaaggatac caaaatttat tatcctaatt
    42841 cagaagttaa tggagtattg gatactaagc catataagaa tgaatctgaa aggtcgtggt
    42901 tattcagagc tgtaggtacc ggtcatgaca gacaggtatg gaatgatata tttgcagaac
    42961 tgagaatggc aggttatgat catgtaatat caatagaaca cgaggacagc ctgatgagcg
    43021 gtcaggaagg tttaaagaaa gctattgctt ttcttaagga gactatgata tttgaaccaa
    43081 aaggagagat gtactgggca taagaaaact tatttgtaaa tataagtaat gataaataaa
    43141 atggataggc aacacttttt caaacaactt tcttaagggt atcattcctg tattcg
//
LOCUS       DAOSXM010000011        41960 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2111
            NODE_593_length_41960_cov_13.007493, whole genome shotgun sequence.
ACCESSION   DAOSXM010000011 DAOSXM010000000
VERSION     DAOSXM010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864178
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 41960)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 41960)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:24:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,261
            CDSs (total)                      :: 2,229
            Genes (coding)                    :: 2,218
            CDSs (with protein)               :: 2,218
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 3 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41960
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_593_length_41960_cov_13.007493"
                     /isolate="AMR_MDS_2111"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            125..814
                     /locus_tag="PLQ68_02950"
     CDS             125..814
                     /locus_tag="PLQ68_02950"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HXK71640.1"
                     /translation="MGKDPNKNIKMRQESINRILDSALSVYLRCGYTAADMNEIAKNA
                     NIAKGLMYYYFKSKQELFKTLFKTMFKNAMDLSEKALIESEDMPPVEKLAYFIYVIFG
                     EGSKNPQLIRFFMRFPFDTYAVFGPDNWHEAAQDSDLFRKSICEIIEKGIEKNDIPYT
                     NADNAAHSFWTVFVANLFRYSKMMEGKEQDEISSIQNVASFCFRGLMIPDEVWQKALK
                     NIVPRKEHNND"
     gene            807..1697
                     /locus_tag="PLQ68_02955"
     CDS             807..1697
                     /locus_tag="PLQ68_02955"
                     /inference="COORDINATES: protein motif:HMM:NF015081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HXK71641.1"
                     /translation="MIKSFKNEKGKQEVFRTYNDLVEQWGIPVEDKAIYGKYGSTHVI
                     IAGKEDNPPLLLFHGVGDNSALMWIFNAKELSKHFRIYAIDAMGGAGKSVSNDNYEKD
                     FNVLKWLSTLLDSLKISKAFAVGVSYGSLIVQQLLCYMPDKVIKGVCLAGGSASAEYS
                     KKNSALKKMKIFLPEALFPTKSNCIKLVKKLGGENSIKLIDNKSFFTHWCLLLKYFNN
                     TSMMKHNVKPIEKEKYLVTKDNCLFVIGDKDKIAYSEDSVKALQQSDLNYLIIEGAGH
                     PINHEYPDKINSLIIDFLLK"
     gene            complement(1700..2554)
                     /locus_tag="PLQ68_02960"
     CDS             complement(1700..2554)
                     /locus_tag="PLQ68_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014794500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXK71642.1"
                     /translation="MIVFTKRNLMVFFKDKSAVFFSLLATLIIVALYLLFLGDTWSNS
                     FQGVEDPRTFMDNWIMAGLIAITPFTASMGAFGTMINDKTKKIYKDFYCSSISRFAIT
                     GGYILSSYIVGVILSLVTLIFAQTYILIQGGEIISLLVLLKIIGMIFLSTFMSTAVVL
                     FLVSFFNSENAFATASTVIGTLIGFLTGIYIPIGVLPEAVQVIIKVFPVSHSAVLLRQ
                     IMMEKTMNEAFINLPSEAVVEIKELLGVTFKLNENTVTPIIHLIILVVTGVIFLGLSM
                     LSMSSKKK"
     gene            complement(2551..3465)
                     /locus_tag="PLQ68_02965"
     CDS             complement(2551..3465)
                     /locus_tag="PLQ68_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018211375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXK71643.1"
                     /translation="MDKIIEVKNLSKSYGSIKAVKDISFYVEKGKLFAFLGPNGAGKS
                     TTIDIICTFLKKDSGNIVIDGLELGKEDDEIRGRIGAVFQDGLLDNLLTVEENLMIRG
                     GLYKNKKEDLKTAVDEIAEIMGITEILKRPYGKLSGGQRRRCDIARALINKPQILFLD
                     EPTTGLDPQTRKSVWETIQRLQKSNNMTLFLTTHYMEEAAEADYVIIIDEGQIAAKGT
                     PAFLRETYAKDKLSLMCRDVKTVSGLLAQKNIENVIISDCVCVELKETMEALQIIQDV
                     KEYITGFEVTAGTMDDAFIGITGKEIRS"
     gene            complement(3478..5049)
                     /locus_tag="PLQ68_02970"
     CDS             complement(3478..5049)
                     /locus_tag="PLQ68_02970"
                     /inference="COORDINATES: protein motif:HMM:NF028272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC exporter domain-containing protein"
                     /protein_id="HXK71644.1"
                     /translation="MKTNAIPYLLLTKLKNNIKQLIKNPAKLIYAIVLIVFIGFSFFA
                     KNQAENENTSYRNIKELTAGITALYIVIFTLISYNGFKNGASMFKMADVNLLFTSPIH
                     PRKTLFYGLFQQMGTSLLLGFFIIFQFAWMNMVFGVTFGQLIFILLGYSLSVFAAQLT
                     AMVIYSFTSYDEKRKKIAFTIYFGLIIVYVIGLLMYLLGDMNNIVPNLVNAGNSIYIR
                     LMPAGGWIGGIVNGILQNNIQMILTGSLITVGYFVGMMFLLSTAKQDYYEDVLKASED
                     QQILSSAQKEGRSSEAFSKVKVGKTGLTKGDGASTFYYKHKLEDRRIKKFLLDPISIV
                     FLFVIIIFSIFVRKFGIVYIFAYAAYMQLISVKMGRFSKELAKPYIYLVPESSFKKIL
                     YSIAESFAGFLTEGVILFIALAFIMKLTPYDAVILIIARLSFSYLFLAANILIQRVFG
                     TLSSRTLIYLFYIFAVLIMAAPGVILAAILSSSGLFLLTPNVIPFLIVTLCNIPITLL
                     VIYLCRDVLKYAEMK"
     gene            complement(5049..5756)
                     /locus_tag="PLQ68_02975"
     CDS             complement(5049..5756)
                     /locus_tag="PLQ68_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006356148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXK71645.1"
                     /translation="MLEISNVTKKYGKTVANDNISFHVNDGQIAVLLGPNGAGKTTII
                     KCISGLLRFKGDIKIDGYDNKSIDAKKLLGYIPEMPAVYDLLTVEEHLEFIARAYKTR
                     DDEYEKELLERFELWDKKGKLGKELSKGMQQKLSICCALVHKPKVIIFDEPLVGLDPH
                     AIKVLKAMFVELKESGCTLLISTHMIDSVEDYWDVAHIMVNGSFAATKVNENGNDKSR
                     EKLEELFFEITEKKREE"
     gene            complement(5792..7405)
                     /locus_tag="PLQ68_02980"
     CDS             complement(5792..7405)
                     /locus_tag="PLQ68_02980"
                     /inference="COORDINATES: protein motif:HMM:NF013752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HXK71646.1"
                     /translation="MSISKYSLIDVPSGDGVHTKTAGAKGEKYVYKYTHYFRNAEGKP
                     RNRAILIGKIDAETGKMEPNSNYYEIFKITPGMLDAAVWDYGYSYLVLKCCNDMGLTA
                     CLNKVFGSQTMDIIISAAYIVREGSSMDAIDDWLERTLVPGCSKSLNSQSTSRLFESI
                     SLSKTHEFFKKWVAVGRTGENICYDVTSISSYSRTMTDVEYGYNRDGEDLPQFNIGMF
                     CDESSKLPLYFNRYNGSLTDKTNLSYVLENAGAVGLKDIKFVVDGGFISEECIRNLST
                     HSKAFTIGIPAYLDIAVEMLKSHSQGIEKYANKLSDKDIYCVDKHFEYYGTSGRLMLF
                     YDPMNHAQLCNEMSERIRSLSAELSGLKRYPASRLKRYTQYFRITKHADGNGFDFAVD
                     VDKVDLLRQYKGFFLIFSTDNEISHEDVLYHYRAKDAAEKLFDQLKIDMQGARIRTHN
                     EQTTNGKAFVLFIALVIRTYIMGKLKKHLDEKSTSLKKVFNQLTNITVITTGGKARFT
                     KALTKEQKSILSAFNATDDILKSVETCLR"
     gene            complement(7768..8790)
                     /locus_tag="PLQ68_02985"
     CDS             complement(7768..8790)
                     /locus_tag="PLQ68_02985"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HXK71647.1"
                     /translation="MSNEVIRYGIIGCGGIANTHAEALQQIKGAQLVGVSGCSAEEPV
                     SFAKKFGIKSFDSTDLLLQSKKIDAVTICTPSGYHASIAISAAEHKKHAVVEKPLSLT
                     AESAKRVVEAFKNNKVKGSVISQLRFSEEIRKAKQIISSGRLGKIIMTNLNMKYYRSQ
                     EYYDSGKWRGTWALDGGGAMMNQGIHGIDMLLFLIGETKNIYGYIRTLTHKIEVEDNA
                     VAVIEFKDGSLGTITASTSMNPAQNKTIEINGTKGTILIEEDRICRLYIENEEDYTAD
                     DITMGDSPASHIIKATGHIKQLTDFTQALLEGREPFVSLEDGMRAVDLITSVYASSKT
                     NEIFNK"
     gene            complement(8787..10124)
                     /locus_tag="PLQ68_02990"
     CDS             complement(8787..10124)
                     /locus_tag="PLQ68_02990"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HXK71648.1"
                     /translation="MSELAIFGGKPAVDEKIIPESLFKWPIVTKEDEEAVLEVLRRGA
                     MSGLDVTEKFEKEFAQWQKRKYAIGYCNGTMALQAAMYACKMGAGDEIICPSKTYWAS
                     CLQAFNLNSTVVFADIHPTSLCIDPNDIERCIGPRTKAIMVVHYLGHPADMDPIMEIA
                     KKHNILVIEDVSHAQGGMYKGRMLGTFGHIAAMSIMSGKSLAAGEMGMLATDDKEMYD
                     RAMAYCHYERNNSKYITTEYLQKYASMPLGGVKGRVNQLSSAMARVQLKYYEERTAEI
                     RKAMKYFWSLLEGVPGLRAHMVDESDGSNMAGWYAPHGLYCPEELEGLSVERFCDAVR
                     KEGFGASPGANKALHMHNIFKDYNGLNFEKPARIAFTDRDVRELDKELMVSEQVQVYS
                     IPWFKHYMPEYIEMYANAFKKVALNYKELLAGDEKRGTESGRWFFYSDKKEEK"
     gene            complement(10223..11131)
                     /locus_tag="PLQ68_02995"
     CDS             complement(10223..11131)
                     /locus_tag="PLQ68_02995"
                     /inference="COORDINATES: protein motif:HMM:NF014376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HXK71649.1"
                     /translation="MADKNKINKIPVFKVEDNPDGYLMFYLKKYAPEEGTLYLHAHEC
                     IQINYVYSGKAIHIIHDKRIELVKGDIFVIPPNVPHQIEPVIGTDAKIVEFEFDPKFI
                     NEFFKDLDKGEAFWDFAYIEPFLVEEDLVKPRLNLVGSIQTDSENIFNEALKEYEKRE
                     PGFDLIIRSLLLKLLVLVGREFTKNISKSKNAQIFERHKDAIKGAIRYIEENYTQDLN
                     IDSVAKMFLLSPSFFGYLFKNLTSKTFKEYITGLRIAKASEMLKNTDEKIITICYESG
                     FNNINHFNRTFKQVVGLTPGQYRKNN"
     gene            complement(11132..11815)
                     /locus_tag="PLQ68_03000"
     CDS             complement(11132..11815)
                     /locus_tag="PLQ68_03000"
                     /inference="COORDINATES: protein motif:HMM:NF014373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HXK71650.1"
                     /translation="MNDIIQYLRELSPATMVIRLVLAMLAGGLIGMQRERHGRAAGFR
                     THIIVCIGSTMAAMTGIFMTQTMGFDSDPMRLGAQVLSGIGFLGVGTILVTGHLHVKG
                     LTTAAGLWTIAAVGLAIGAGFYEAALVCVLILAVVMTFLGNFGSRRIKMDVYLEVTGS
                     RNINSVIKTIEETGYLIDNIEVHPAKSGTYGNIGLSIVVGIPKDSKDEEVIDSIANVE
                     NVVLVVRCD"
     gene            complement(11830..13740)
                     /locus_tag="PLQ68_03005"
     CDS             complement(11830..13740)
                     /locus_tag="PLQ68_03005"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016294206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HXK71651.1"
                     /translation="MHYGFIKAAAASPMLKVADCKYNCDNIIKTMDYAYKRGVNILVF
                     PELNITGYTCDDLFAQKLLLDQARISLIELADKSSGIDMLSIVGLPLEVDGFLYNTAA
                     VLCKGEILGIVPKTYIPNYNEFYEARRFSEAPKNTIYLDLEGKQIPFGTKLIFQNLQM
                     EEFTLAVEICEDLFVPVSPSSEHAMNGAMIIANLSASNEILGKSEYRKTLVKAQSGKC
                     FSAYIYSNSSYSESTTDIVFSGHKMIAENGVILAEEPPFGKEYLEADIDLSRLAHDRV
                     NQVSVRKDWTERGYMYIPFNMDLRETKIDRLIEKHPFIPKDTSVLMERCNQIFNIQSA
                     GLKKRLIHSKLDTSVIGISGGLDSTLALLVTHRAYIEMGRDTKGILCVSMPCFGTTKR
                     TKDNAKKLCALLETDFKVIDISKSVKSHLSDIGHDCKTTDTTYENAQARERTQVLMDL
                     SNMTNGLVVGTGDLSEQALGWSTYNGDHMSMYNVNTSIPKTLVKYIIRYVADDAGGKL
                     KDVLYDILDTPISPELVPAVNGEIVQKTEQIVGPYELHDFFLYYFMRWGFTPQKILML
                     TKKAFKGDYSEEEIKKWLSVFIKRFFIQQFKRSCSPNGPKVGSVALSPRGDWRMPSDA
                     SYEGWISDLENS"
     gene            complement(13813..15153)
                     /locus_tag="PLQ68_03010"
     CDS             complement(13813..15153)
                     /locus_tag="PLQ68_03010"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXK71652.1"
                     /translation="MNAYLHAIKIKWRNSAFVRTFNLHDEKAKGRSCFLASSVLASLA
                     SQLSGGIFYTGFLLSYGINIVNIGIITFVPTITRLLSMFSPFILERFRRRKAILTTLS
                     IIANIINILGITLLPNIVTTEAGRVIGFTLLVTMSSAISAIIDPGYSAWHSTFLPNEI
                     RADYFTLSSGINSFLTQGIVLVLSLITDSFAGSPQQLVIITGFRYAALVVGILHVVAL
                     AIPKEYEYVRSKSKIKISDIFTLPFQNKKFLLTVLLVAFYYIGSSLTNASINIFLLQD
                     VGISYSEINLINAVYFMFFIFFGKMWKKFLKKHSWFKTFAFVMFLQGPTYLMYAFVTP
                     ENYTWLWFGIRLLQHVLGVAANTVLASMLYVNLPKQDQTNYISFYNIFVHTSIFLSMM
                     LGTFFLSGMDGNVLNIFNFEMAGVPILLAVTGVAQIIAGGLFLKFNKTLIPDDL"
     gene            15439..16908
                     /locus_tag="PLQ68_03015"
     CDS             15439..16908
                     /locus_tag="PLQ68_03015"
                     /EC_number="1.11.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018360855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HXK71653.1"
                     /translation="MEEKKPKKKKLTTDVGAPVANNRDVLTAGRRGPMLLQDVWYLEK
                     LAHFDREVIPERRMHAKGSGAFGVFTVTHDITRYTKAKIFSEIGKKTDMFVRFSTVAG
                     ERGAADAERDIRGFAMKFYTEEGNWDLVGNNTPVFFFRDPLKFPDLNHAVKRDPRTNM
                     RSPTNNWDFWSSIPEALHQVTITMSDRGIPYSYRHMNGYGSHTFSMINDKNEIVWVKF
                     TLKTQQGIKNLTDQEAEAVVAKDRESHQRDLYEAIERKEYPRWTMYIQVMTQQQAKKM
                     KNNPFDLTKVWFKKDYPLIEVGYFELNRNPDNYFAQVEQAAFNPANVVPGIGFSPDRM
                     LQGRLFSYGDAQRYRLGVNYNNIPVNAPKCPYHSFHRDGLMRTDDNQGSLLHYYPNSF
                     GEWEDNKEHEEPPLDIDGDAFRWDYHEDDDDYYTQPGKLFNLMTKDQQQVLFENTARN
                     MDTVPQFIKIRHIKNCAKADEAYGRGVAKACGIDYESIE"
     gene            16937..17509
                     /locus_tag="PLQ68_03020"
     CDS             16937..17509
                     /locus_tag="PLQ68_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014826685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HXK71654.1"
                     /translation="MERVFKYDIMQEIKDRWSPRAYSEEMISKDDLYAILEAASFAPS
                     CFNEQPWRFVLADDEQSIKKMRSILYSQNLKWAEKAPVLLLIAAKKTFSLDGEQNFWH
                     MFDTGTAWGFLSLEAQKRGIITHAMGGFSKKKARKEFNIPDDLEIITVVAIGRYGDIN
                     QLEDELREREKPETRQDISRLIINKEPYHD"
     gene            17502..18341
                     /locus_tag="PLQ68_03025"
     CDS             17502..18341
                     /locus_tag="PLQ68_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012414513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HXK71655.1"
                     /translation="MIRKPLQTITGINTSDGAGVKLVRVIGHRNVYDFDPFLMLDAFD
                     SNDPKDYIKGFPWHPHRGIETVTYLISGDIQHGDSLGNEGRILDGCCQWMTAGSGIMH
                     QEMPQPSRKMLGVQLWVNLPAKNKMTSPKYHDIEAKDVPLIKKDDYEVRVISGSFDNV
                     KSKDNDEFVKTTFLDVLLKPDKPFEIETAPDSTLFLYIVYGDGYFADLDELFSSKRAV
                     LFDKGEIFHAKAGKEGIRFLLFNAKPLKEPISWGGPIVMNTKEELQKAFDDLSNGTFI
                     KKN"
     gene            18440..20971
                     /locus_tag="PLQ68_03030"
     CDS             18440..20971
                     /locus_tag="PLQ68_03030"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HXK71656.1"
                     /translation="MQEQFYENLIREIPVAYAYHKMIYDESGKPIDYEFLDVNEAFEK
                     MTSLSSSQIIGKRVTEVIPTIIEDSFNWIEEYGKIAQSQISNEFETFADTLQRYYRVF
                     VSSPKKGYFTTIFLDITKTIIKTQEQLALLTMLNDIFIEMDENYKIINVLAPDNILDR
                     PKNELIGRYMYKISPTEFFDKIKHYSDLAKKKKTKVTFEYSFSVKNKVRWYRSDCLYT
                     KILNEERIVFFISDITEKHDLENKLTQSESLFRALFEQAPIGVALVKNFKFMSNINDK
                     FVEIMGMTRKQLISTDWKDITHPDDLEEDLKYFNLFKAGKIPSYSMVKRFIRSDRSVV
                     WVYMVITYLMLDEYEERKDTHVCMINDITQIMEMNDALKESERSKSVMLSHLPGIAYR
                     CAIDKDWTMHFISDGCYELTGYPPDAVLNNKEISFNEIIAPEYRDMVYKQADEVAKNK
                     TYFKAEYEIIASDGSRKWVYDQSQFFYDNNDNPIIMEGLIIDITENKKRELHIAYLNS
                     HDMLTGLYNRIHFMSEIQRLDNKDHLPLSIIIGDINGVRLVNNAFGTKRGDRLLLRIA
                     KIIKSNCRPDDIVARIGGDDFAVLMPNTNNATAEAVTEKIRMECEKLANKPLKTPVFT
                     NISLGFGTKIKNSEDINQIFREAEDLMFKRKLLDRKSSHSDILAAILASLFAKSEETE
                     EHAQRLYELSKKIGNHFHLNSRDLDLLQLFTLLHDVGKIGIDDSVLKKPGKLTDKEWI
                     EMKKHPEIGYKIAISSPELEPIADLIYTHHEHFNGKGYPQGLKGEEIPLLSRILSVAD
                     AFDAITHDRVYRKAASIEEAISEINKYSGTQFDPKVVEALNNIYI"
     gene            21043..21435
                     /locus_tag="PLQ68_03035"
     CDS             21043..21435
                     /locus_tag="PLQ68_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020072227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin family protein"
                     /protein_id="HXK71657.1"
                     /translation="MKNEHQKFFICRHCGNIIGMIFNSGVPIVCCGEEMEELVANTTD
                     GAHEKHVPVVKKEGNLVHVEVGSVPHPMLKEHYIMWIYLETEKGGQRKSLMPGEKPEV
                     TFAVTDDDKAIAAYEYCNLHGLWKSDVK"
     gene            21480..21638
                     /locus_tag="PLQ68_03040"
     CDS             21480..21638
                     /locus_tag="PLQ68_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010966063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HXK71658.1"
                     /translation="MKKYVCTVCGYIYDEADGDPDNGVEPGTKWEDVPEDWVCPLCGA
                     SKEEFEEE"
     gene            21920..22921
                     /gene="aroF"
                     /locus_tag="PLQ68_03045"
     CDS             21920..22921
                     /gene="aroF"
                     /locus_tag="PLQ68_03045"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011877155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HXK71659.1"
                     /translation="MIAVLKPNAPQAQIESLITWFKDQGLNVHVSVGDYQTILGLIGD
                     TSKIDVELLEVLDIVESVKRISEPFKNANRKFHPDDTIIKVKDVTIGGGNFVMIAGPC
                     SVESESQVLEIAQKVQAAGANMLRGGAFKPRTSPYDFQGMKAEGIELLLKAKQRTGMP
                     IVSEIMNANHIPLFEDVDIIQVGARNMQNFELLKELGKTNKTILLKRGLANNLKELLM
                     SAEYIMAGGNENIILCERGIRTFETYTRNTIDLSAIPMLKELSHLPVIVDPSHATGMA
                     KLVRPMALAAAAAGADGLMIEVHNDPLHALCDGMQSLTPEQYAEVADKVRKILEVIK"
     gene            22918..23754
                     /locus_tag="PLQ68_03050"
     CDS             22918..23754
                     /locus_tag="PLQ68_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005949197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HXK71660.1"
                     /translation="MKIGIVGLGLIGGSLAKTYKSNSDNEIYGYDTNKPVLDIAKMSG
                     VIDGVLDVSKISMCDCIFIALYPKATIDYLEKNAQYISKNAIVIDTCGTKRKICETGF
                     SIARQHGFVFAGGHPMAGKQNSGYKYSTSTLFKDAYMIVVPDSTDNIRALERIKKILE
                     PLSFKHITITTAQKHDKMIAFTSQMAHIVSNAYIKSPTAKAHKGFSAGSYKDLTRVAY
                     LNENMWTELFLENKDNLANELNLLIEQLYEYKKAIENNDDMKLKELLEQGKECKEEVD
                     GR"
     gene            23751..24815
                     /gene="aroB"
                     /locus_tag="PLQ68_03055"
     CDS             23751..24815
                     /gene="aroB"
                     /locus_tag="PLQ68_03055"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HXK71661.1"
                     /translation="MKRVIVKSSKKYSIIIGKGLLENISDHILPILKGNNVYIITDDI
                     VESLYISQLIKSLQKNNLVVHKYVFPNGEKSKNAKNYIDILNDMAQKGISRSDTVIAF
                     GGGVPGDLGGFVAATYLRGIKLIQIPTTLLSAVDSSVGGKTAIDLDKGKNMAGVFYQP
                     DLVVCDLSSLNTLSDETLKDGCAEVIKYGILSGGLLFNQLKKPIMKHIESIVYQCVKY
                     KRDIVQKDEHDQYLRQLLNLGHTIGHAIELLSGYKVTHGQAVAKGISVISKISFNLSL
                     CAKHTVDEIDNILTGYGFDLNPMYSPSDLASAIIMDKKRCNDSINLILVEDIGKCIIR
                     KTMMNELEDMIKKAITKVPL"
     gene            24812..26104
                     /gene="aroA"
                     /locus_tag="PLQ68_03060"
     CDS             24812..26104
                     /gene="aroA"
                     /locus_tag="PLQ68_03060"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR01356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HXK71662.1"
                     /translation="MNISLTINNPTGEINAIASKSYAHRHLICSALSDKPNYLKINQS
                     SQDIEATINCLNALGATIVKNDNGITISPISKIIKNACLDCNESGSTFRFMLPISCAL
                     NSESVFQMKPGLARRPITNLYLQLKSKGCILSEEGSVPFYANGQLQGGDFSLPGNISS
                     QFITGLLMALPLLNLKSTITLTSDLESSGYVDITIDVLKNYGVNIEKKFNKFIIPENQ
                     KYISPGNADIEKDWSNAAFFLCAGALTDKGITVTGLNRNSHQKDMYILNVLKDIGADI
                     SFSENKITVKKNKLNSFELDAKEIPDIIPVLSLVASLSKGTSRIYNAKRLRIKESDRL
                     SSIYDMLKKIGAQIEQNDDTLVITGKPMLKGGIVDSFNDHRIAMTAAIASISCENPVI
                     IKDAQAINKSYPSFLDDFIKIGGKVETVSGGNDVFSIR"
     gene            26085..27176
                     /gene="aroC"
                     /locus_tag="PLQ68_03065"
     CDS             26085..27176
                     /gene="aroC"
                     /locus_tag="PLQ68_03065"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HXK71663.1"
                     /translation="MSSVFGNNLKVSIFGQSHSKAIGVCIDNLPPGFKIDMDKINAFM
                     KRRSAQNKAYATKRNEPDEIEFVSGLVNGMTAAAPLCALIFNKDFKPKDYENLKNIPR
                     PSHADYTSYVKYKGFQDPTGGGHFSGRITAALCIAGAICKQILESEGMTINAHIYKLH
                     GISDQPFDSSGNNKKQITHLYSNDFPVIDIDKGNLMLDEIKKASDKSDSIGGIIECII
                     EDVPAGIGDPIFDGIENNISKAIFSIPAIKGIEFGNGFLCADLNGSDNNDAFCIKDGK
                     VKTKTNNHGGALGGISSSMPIIFRVAVKPTPSIGIEQDSINLDSMKEEKLNINGRHDP
                     CIVPRAVPCVEAAASIALLDMYMSYKIWR"
     gene            27181..28323
                     /locus_tag="PLQ68_03070"
     CDS             27181..28323
                     /locus_tag="PLQ68_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009140500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase domain-containing
                     protein"
                     /protein_id="HXK71664.1"
                     /translation="MTIDKYRDEITAIDDKLLELFHKRMRIAEKIAQYKKENNIPILN
                     TSVERAKLAELSEKADDDMLFYSRILFNTLFEMSRSYQEKLNHPKSVLMQKIESAIEN
                     TNRLFPSTGNIACQGVEGAYSQLACDKLFDSPNIMYFTTFEGVFSAIDKNLCKYGVLP
                     LENSTAGSVNKVYDLMMRYKFNIIRSVRLKIDHNLLAKKGVKKNEIKEIYSHEQAISQ
                     CSEFIKQLGNVKINVCENTAIAAMMVANSERFDIAAISSRPCAELYGLNCVSESVQDK
                     GNNYTKFICISKDIEIYPGADKTSIMIVLPHKPGSLYRVLARFYILGINLVKLESRPI
                     PDRDFEFMFYFDIETPVYSEQFTQLINELDEMSEDFRYLGSYLEII"
     gene            28320..29543
                     /locus_tag="PLQ68_03075"
     CDS             28320..29543
                     /locus_tag="PLQ68_03075"
                     /inference="COORDINATES: protein motif:HMM:NF013375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HXK71665.1"
                     /translation="MKKYGLLGRKLGHSYSPIIHSFLGKYEYALYEREPEQLNEFFSD
                     STVSGFNVTIPYKKDALKYCKIITSIAERTKCVNTVIRKNGNYLLGENTDYYGFMYMI
                     KKSKIAIEGKKVLLLGDGGSAATIKVVIEDLNAKNLVTISRRGPVNYENIHLHYDSDI
                     IINSTPVGMFPNNGEAVIDISKFKNCIALFDLIYNPAQTKLMYDAKQLGIPSYNGLTM
                     LVAQAKKASELFTGSVIDDSVIENIIDKVSKKSMNIVIIGMPGSGKSTVGRALAKLTD
                     RCFIDTDDEVSRTYHKDTAEIIVNEGESVFRDLETQILENVTKKSNCVISTGGGIVTR
                     ERNKYLIQQNSIIVYLKRDISLLSTYNRPLSQINDLKKLFESRVDKYEGWSDYQVDCD
                     DISNTHIKIKEMLGL"
     gene            29540..29968
                     /gene="aroQ"
                     /locus_tag="PLQ68_03080"
     CDS             29540..29968
                     /gene="aroQ"
                     /locus_tag="PLQ68_03080"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005957667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HXK71666.1"
                     /translation="MKLLVINGPNLNMLGIREPAVYGSSTYNDLVDYINTVCSKNDIQ
                     CEIYQSNHEGAIIDKIQSAYKVFDGIVINPAAYTHTSLAIADAIKSVGIPTIEVHISD
                     ISKRESFRQFSYTSMVCIKTIMGKGFLGYEEAITELKNII"
     gene            complement(30207..32846)
                     /locus_tag="PLQ68_03085"
     CDS             complement(30207..32846)
                     /locus_tag="PLQ68_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004798152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HXK71667.1"
                     /translation="MIAHFEKKEEIIKQLETDAVNGLSSTQAAAKLEQYGKNKLREPK
                     KKSIAQRFLDQFKDVMIIILIIAAIISFIVALYEKEAKEFLEPVLIMLIVILNAIMGV
                     LQESKAEKALEALKNMSSPNARVIRDGEEQVIEASLLVPGDIIRLEAGDFIPADARII
                     RSSSLKSEEAALTGESVPSEKDADEEVAENAPLGDRTNMVFTGCSITYGTALAVVTTT
                     GMDTEMGRVANLLESEESTQTPLQKKLASLGKYLGIVALAACAVIFIVGIMDGMPVME
                     IFMIAVALAVSAIPEGLPAIVTIVLSIGVQRMVKKNAIIRNLPAVETLGSASIICSDK
                     TGTLTQNRMTLVEAYYDGMEKTEKITVNNSPDIKKLLKFGTLCSNGSVVFTKDAEKHI
                     GDPTETAIVYAAHRNGLEKEDLNRSYPRKAEIPFDSDRKLMTSINVMDDKTVVIVKGA
                     VDVLIERCIEGDKEKAKDINYTMSKNALRVIAVAYKYIDSIPKELKPEDIEKDLIFMG
                     LVGMIDPPRPEAKDAVAVCRKAGIKPVMITGDHVITASAIATELGILRENDEAITGIE
                     LDNMTDSEFEKRVRNISVYARVSPENKIRIVRAWQRLGEVVSMTGDGVNDAPALKAAD
                     IGCAMGITGTDVAKGAADMTLTDDNFATIVDAVREGRGIYANIKKVVGFLLGTNIGEI
                     LTVFFAMILWRETPLLSMQLLWINLVTDSLPAISLGMEDVESDVMEQKPKPRNEGIFA
                     HGYGIRIILQGCMIAAITIGGFIYGRNVIGTLEAGQTMAFFVLSLSQVVQSFNMRSEH
                     SLFKIGFFTNKRLNIACLISAALIALVLFTPLSTAFQLISLDLNAYLIGLALSFVPLF
                     VMEISKALGLIKPKKIMVEQKDK"
     gene            complement(32967..33659)
                     /gene="araD"
                     /locus_tag="PLQ68_03090"
     CDS             complement(32967..33659)
                     /gene="araD"
                     /locus_tag="PLQ68_03090"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009001516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase AraD"
                     /protein_id="HXK71668.1"
                     /translation="MFELLKKEVYKANIELVKKGLVLYTWGNVSAIDEERKYIVIKPS
                     GVDYDEMMSEDMVVVDMDGNVVEGKYRPSSDTKSHIEIYKAFINTHAVAHTHSTYATS
                     FSQAAKGIIPMGTTHADYFNGEIPCTRALLSDEIKNDYELNTGKVIVETFTGKDYNAI
                     PACLVRNHGPFTWGDNAMEAVFHSVVLEEIAKMAFITEKANKDIQSINKLLLDKHFKR
                     KHGQSAYYGQTK"
     gene            complement(33735..34793)
                     /locus_tag="PLQ68_03095"
     CDS             complement(33735..34793)
                     /locus_tag="PLQ68_03095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK71669.1"
                     /translation="MDKNFHIGWQTGITYESTEKKGVSMSKMLMLLDEMAEHDMNMLS
                     LMMVSYSYFDPMHDGLCWPVRDKRLKHLCDKTCTNANMDTEFVSKIIEEAEKRGIGIQ
                     LFTNLGIYNPKRIIKSYPKANEQINKDGDIYKWLFCPDSPGAWQLELDEISDLLKLYN
                     HENVRSISYERLSYAGGSCFCDYTKLLFNQDTGLKLSDYSRDDEIFEKWKIEKITEKL
                     KTMNDCIRSINQDIEICLHTSLAKGWGHEVRKLKGAGVDCIMPHIAHFPISQTEFNNL
                     LDSIYPNDIILHVCVRNKSLSNYDIWIKTPQIIQNIGNWIREYKEDHSNLKDVVFFNE
                     NTVSDENRRAVYQLISDF"
     gene            complement(34902..35738)
                     /locus_tag="PLQ68_03100"
     CDS             complement(34902..35738)
                     /locus_tag="PLQ68_03100"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HXK71670.1"
                     /translation="MSDTLQKLWDTLIELSATVGLRILLAIAVLIVGLLLIKYIVKAI
                     VKSKGFIKIDKTVQTFTKSALSITMKLLLIISVIGILGVPLTSIIAVLASAGLAIGLA
                     LQGALSNFAGGIMILIFRPFRVGDFIDDGTHMGTVDSITIFYTKLLTIDNKLITIPNS
                     TMTSASVTNFSAKDKRRVDLKFTASYTNDVDKVNSILLEIAKNHPMVDQDPPVMAKLF
                     THGNSSLEYILRAWVDAPNYWTVYFDITEQVKKEFDKQGIEIPYPQMDVHIKENQIKE
                     KE"
     gene            complement(35813..37372)
                     /locus_tag="PLQ68_03105"
     CDS             complement(35813..37372)
                     /locus_tag="PLQ68_03105"
                     /inference="COORDINATES: protein motif:HMM:NF019874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HXK71671.1"
                     /translation="MDYLRKKYRILLISIILVIGLIISGVVYFVLIDLEEAYHEYAKD
                     SIMDVKKGYLKDTVNNIIDGIEQKNENQVIYHQHLAEDMIGIFDNYYLADSEGFLYYA
                     KQYMQQEAKKIDFTFLIINSQNNEVVFQYIPSLEDENIISTEYINHLSSELPVYTSKL
                     YGNYKTVIGVSQDVIDSRVKNALYNEIHGLKFEDDAYVWVNEIVNYAGGDDYAIRRIH
                     PNLKDTEGIYLSTNMTDVMGTTPYKTELEGVKQYGELFFQYYFKKLNSDTISEKITYA
                     KLYKDFDWIIAMGVHLDDIQEYVDKTTQDKSQQVRMLLIILSSIVIVIIIIALVVVHH
                     LERWYYGNFNKTLIEESLKDPLTNTYNRRAAYKYLATSWANFKSVGLNAKIMMIDIDN
                     FKKVNDSCGHSVGDQVLIKLADTINSHIRSNDFLSRWGGEEFLLICNGLKKEDVSNFT
                     KKLLVTVEEMQFECEEAKKFNITVSIGVSDFYSSDDQFSIAVNRADMALYKAKAAGKN
                     TFVVLEETTEN"
     gene            complement(37510..37983)
                     /locus_tag="PLQ68_03110"
     CDS             complement(37510..37983)
                     /locus_tag="PLQ68_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008426026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK71672.1"
                     /translation="MLTRKATSKDIKQWKRIWNEYKDKLKPNRKSGNEIIEYLMKKYC
                     LEVINDEKLSEVVKSNIMNNKCHLEKLPSGTTPDIKTFYIINTDKSKSLYINRPDIYK
                     DMRIFIGIDQVTGFFHIEGSETLWDELYCFRGLDEKDIQNPFLVAEYIKCMDGNI"
     gene            complement(38043..39215)
                     /gene="tyrS"
                     /locus_tag="PLQ68_03115"
     CDS             complement(38043..39215)
                     /gene="tyrS"
                     /locus_tag="PLQ68_03115"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012505290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HXK71673.1"
                     /translation="MKNEKVMEILMRNAVNVFSEEGLRKKLESGKPLKVKFGADPSRP
                     DLHLGHTVPLRMLKVLQEAGHQIIFVIGDFTGMIGDPSGKSKTRNSLSFEETRKNGQT
                     YFEQVAKVLDPKKTQIVYNSEWLGKMDFADVLNLAGKYTLARIMERDDFSSRYSNNQP
                     IGIHELLYPLIQGYDSVAINADIEIGGTDQTFNLLVGRELQKDYGQEPQEVITFPLIA
                     GLDGIEKMSKSLDNYIGINESAETIFEKCMKIPDNLLNDYFRLTTDIPEEQFSAVVNE
                     DIREGHMVYARKIIEMYHGKDFIKPAEDRYISIACKKIPHEMKVMEIDEDKIQIIDLL
                     RLTGYAKSNSEARRLIEGNAIKIDGDIINDINYAICNDSVICRGKSKFVRIRFKKI"
     gene            complement(39476..39991)
                     /locus_tag="PLQ68_03120"
     CDS             complement(39476..39991)
                     /locus_tag="PLQ68_03120"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002607757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HXK71674.1"
                     /translation="MVIKHNNLVIRDATALDAHILCKWWNDGKVMAHAGFPNGLCTTD
                     EEIIKQLSNDNDEIHRRLIIEHAGIPIGEMNYRNKGNETAEIGIKICDFSKQEKGYGT
                     KLLSMLINSLFTDMGYKKIILDTNVKNTRAQHVYEKLGFKKLRVNENAWKDQLGEYQT
                     SIDYELNKVDF"
     gene            complement(40016..41023)
                     /locus_tag="PLQ68_03125"
     CDS             complement(40016..41023)
                     /locus_tag="PLQ68_03125"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015391476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HXK71675.1"
                     /translation="MGIEKLIDEKFRGCVSVCKDGVRIFERAYGYADLANEVSNDIDT
                     IFATASAGKVFVAVGILMLIEKKILRFDTKISEILDFDLRQIDGNITVEELLTHTSGI
                     PDYFDESVMDEYEELWKDFPNYRIRSNTDLLPLFIDKPMMYPHGSRFKYNNTGFVVLA
                     MIIEKITGKDFDMYLKESIFIPCKMHSTCYYELDRLPAKCASNYIYDEIRSEYRTNIY
                     CVDAKGTGAGGAFTTVNDIKLFWDNLLNYRLLSLDMTENMLKNHSNEEECYGYGIWLQ
                     KIESKYIPYFQGSDPGVSFISSYNKDKKLLITLISNYGDNVWQIHGNIVKDVLTSQEK
                     I"
     gene            complement(41057..41833)
                     /locus_tag="PLQ68_03130"
     CDS             complement(41057..41833)
                     /locus_tag="PLQ68_03130"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HXK71676.1"
                     /translation="MNIKAIVLDLDRTLLKSDKTISDFTLSVLERCHEQGIKIFIASA
                     RPIRAIWEYDKLLNPDAIIWHNGAAVYIERENKYKERISIDDTKNILSNIAKIYPEST
                     LSVEIDDVLYANFDVSVHWENTSAKQTDFTDLPDVASYKIIVGVSSMEQIEKIKDILP
                     DGVYIEMSDKKLGLMMHKDATKLKGIKKALDYYSIELSDTVSFGDDYGDIDMLRACGI
                     GVAVENALPIVKEAADYITETNDDDGVAKWINVHVLANIH"
BASE COUNT        13678 a         7625 c         6807 g        13850 t
ORIGIN      
        1 cttttccaac ctgctcatta tatcagaagc atgaaaaaat gaaatataca acttataaaa
       61 tattgacata taatagatga ataatgtata attgacttat tagtcagtca ataaggagcg
      121 tcttatggga aaagatccta ataaaaacat aaaaatgcgc caggaaagca ttaacagaat
      181 attagattct gctttatctg tttatcttcg ttgcggttat acggctgctg atatgaatga
      241 aatagcaaag aatgcaaata tagcaaaagg ccttatgtat tattacttca aatccaaaca
      301 ggaattattt aaaacactct ttaaaactat gtttaaaaat gcaatggatt tatcggaaaa
      361 agctcttata gagtctgaag atatgccacc tgttgaaaaa cttgcttatt tcatatatgt
      421 aatattcggt gaaggttcaa aaaatccaca attaatcaga ttcttcatgc gcttcccttt
      481 tgatacatat gcagtatttg gacctgacaa ctggcatgaa gctgcacagg attcagatct
      541 ttttagaaaa tcaatctgtg aaattataga gaagggaatt gaaaagaatg atattccgta
      601 tacgaatgct gataatgctg ctcatagttt ctggacagtt ttcgtagcta atctctttcg
      661 atattcaaaa atgatggaag gcaaagaaca ggatgaaata tcgtcaatac agaatgtggc
      721 cagtttttgt tttagaggtc ttatgatacc agatgaagta tggcagaaag cattaaaaaa
      781 tatagttcca agaaaggaac ataacaatga ttaaatcatt taagaatgaa aaaggtaaac
      841 aagaggtgtt caggacatat aatgacttag ttgagcaatg gggaattcct gttgaagaca
      901 aagcaatata cggaaaatac ggttctactc atgtaataat agccggaaaa gaagacaatc
      961 ctcctctttt acttttccat ggtgttggtg acaattctgc acttatgtgg atattcaatg
     1021 caaaagaatt atcaaagcat ttccgtattt atgctataga tgcaatgggt ggagctggaa
     1081 aaagcgtatc aaatgataat tatgaaaagg attttaatgt actgaagtgg ctttctacat
     1141 tacttgattc tttaaaaatc agtaaagcct ttgcagttgg agtttcttat ggaagtttaa
     1201 ttgttcagca acttctttgc tatatgcctg ataaggttat aaagggtgtt tgtttagctg
     1261 gaggatcagc ctctgcagaa tattcaaaga aaaactctgc tctaaaaaaa atgaaaatat
     1321 tcttacctga agcgcttttt ccaacaaaat ctaactgtat aaaacttgtt aaaaaactag
     1381 gcggagaaaa cagtataaag ttaatagata ataaatcatt ttttactcac tggtgtttgt
     1441 tgcttaagta tttcaataat acatctatga tgaagcacaa tgtaaaacct attgaaaagg
     1501 aaaagtattt agtaacaaaa gataactgtt tgtttgtaat tggggataag gataaaatcg
     1561 catatagcga agattcagta aaggcattgc aacaatctga tttaaattac ttgataatag
     1621 aaggagcagg gcatccgatt aatcacgaat accctgataa aattaattca ttaataattg
     1681 attttttgct taaataagat tactttttct tacttgacat gctcaacatg gatagtccta
     1741 aaaagattac acctgtgact actaagatta taagatgaat aattggagtt actgtattct
     1801 catttaattt aaacgtaaca cctagaagtt ctttaatttc aactacagct tcagaaggaa
     1861 gattaataaa agcttcattc atagtctttt ccatcataat ctgacgtaga agcactgcac
     1921 tatgggaaac tgggaatacc ttaataataa cctgaaccgc ttcaggtaaa acccctatgg
     1981 gaatatatat accggttaaa aatccaataa gcgtacctat aactgtagat gcggtggcaa
     2041 atgcgttctc gctattgaag aacgatacta gaaaaagaac tactgcagta ctcatgaatg
     2101 tggataagaa aatcatgccg ataattttca ataaaactag aagactgata atctctcctc
     2161 cctgaataag gatgtaagtc tgggcaaaaa tcaatgtgac aaggctaagt ataacgccta
     2221 caatatatga acttagtata tatcctccag ttatggcaaa acggcttatt gatgaacagt
     2281 aaaaatcttt atatatcttc ttagttttat catttatcat ggttccaaaa gcacccattg
     2341 atgcggtaaa tggagtaatc gcaatcagtc cagccataat ccagttgtcc ataaatgtcc
     2401 tcggatcttc tactccctga aagctattag accatgtatc tcctaagaaa agaagatata
     2461 aagctacgat tattaatgtt gctaataatg aaaagaatac ggcactttta tctttgaaaa
     2521 aaaccataag atttcttttt gtaaaaacaa tcatgacctt atctcctttc cagtgatacc
     2581 gataaatgca tcatccattg taccagctgt aacttcaaat ccagttatat attccttaac
     2641 atcctgtatt atctgtaaag cttccatcgt ttctttcaac tctacacaga cacaatcaga
     2701 tatgataaca ttttctatat tcttttgtgc aagtaatcca cttactgtct ttacatccct
     2761 acacatcaaa ctaagcttat ctttagcata agtctcacga agaaatgcag gagttccctt
     2821 tgctgctatc tgtccttcat caattataat gacataatca gcctctgcgg cttcttccat
     2881 ataatgtgta gttagaaata atgtcatatt attgcttttt tgtaatctct gtatagtctc
     2941 ccaaacactt tttcttgttt gcgggtccag tcctgtagta ggttcgtcca aaaatagaat
     3001 ttgaggttta ttaatcaatg cacgtgcaat atcacatctt cttctctgtc ctccagacaa
     3061 tttaccatat ggtcttttca atatttcagt aatacccatt atttcagcta tctcatcaac
     3121 tgctgttttc aaatcttctt ttttgttttt gtataaaccg cctctgatca taagattttc
     3181 ttcaacagtt agaagattat ctaacagacc atcctgaaaa acagcaccaa ttctgcctct
     3241 tatttcatca tcctctttac caagttccag tccatctatt actatatttc cgctgtcttt
     3301 ctttaaaaag gtacatatga tgtctattgt tgttgattta ccagctccgt tgggacctag
     3361 aaaagcaaaa agttttcctt tttcaacgta aaaacttatg tcctttacag cttttatgct
     3421 cccatatgat tttgataaat tctttacttc aataatttta tccatccatc ctccttttta
     3481 tttcatttca gcatatttta aaacatccct gcaaagatat atgactaaaa gagtgatagg
     3541 tatattacat aatgttacta taaggaatgg tataacattc ggtgttaaca aaaataaacc
     3601 tgaagaagac aatattgcag ctaaaataac accaggcgct gccataatca gtacagcaaa
     3661 tatgtagaat aaatatataa gagtccttga tgacaatgtt ccaaagactc tttgtataag
     3721 tatattagcc gcaaggaaaa gatatgagaa agataatctt gcaattataa gtataactgc
     3781 atcatatggt gtcagtttca ttataaatgc taaagcgatg aataatataa ctccttctgt
     3841 taagaatcct gcaaatgatt cggctatact gtagagtatc ttcttgaatg atgattctgg
     3901 tacaagatat atatatggtt ttgcaagctc tttagagaat cttcccattt taacagatat
     3961 aagctgcata taagctgcat acgcaaaaat ataaactatg ccaaatttcc tgacaaagat
     4021 ggagaaaatg attattacaa ataagaatac tattgaaatg ggatctaaca ggaatttttt
     4081 tattcttctg tcttctaatt tgtgtttgta atagaaagtg cttgctccat caccttttgt
     4141 aaggcctgtc tttccaactt ttactttact gaaagcttca gaagatcttc cttctttctg
     4201 tgcagaagat aatatttgct ggtcctcact ggctttcaga acatcctcat aataatcctg
     4261 tttagctgta gacaataaaa acatcattcc gacaaaatac cctactgtta ttaaacttcc
     4321 tgtaagaatc atctgaatat tattttgaag tattccgtta acaatgccac ctatccatcc
     4381 gcctgcaggc ataagcctta tatatatact gtttcctgca ttaacaagat taggaactat
     4441 gttgttcatg tcccctaaaa gatacatcag aagtccaatt acataaacta taattaaacc
     4501 aaagtatatt gtaaaagcaa ttttctttct tttctcatca taacttgtaa atgaataaat
     4561 gaccattgca gtaagctgtg ctgcaaatac tgaaagggaa tatcctaaaa gaatgaaaat
     4621 aagctgccca aacgtcaccc cgaataccat attcatccac gcaaactgaa aaatgataaa
     4681 aaatccaagt aacagtgatg ttcccatttg ctggaaaaga ccataaaaaa gtgttttcct
     4741 tgggtgtatt ggagaagtaa aaagtaaatt tacatctgcc attttgaaca ttgaggctcc
     4801 atttttgaat ccgttatagc ttattaatgt aaaaataact atgtataaag ctgttattcc
     4861 ggcagtaagt tctttaatgt ttctataact ggtgttttca ttttctgcct gattctttgc
     4921 aaaaaaggaa aacccgataa aaacaatcag aacaatcgca tatattaatt ttgcaggatt
     4981 ctttatcagc tgttttatat tattctttaa tttagttaaa aggaggtaag gtattgcatt
     5041 tgtcttcatt attcttctct ctttttttcc gtaatctcga agaaaagttc ttcaagtttt
     5101 tctctgctct tatcgttgcc attttcattt acttttgtag ctgcaaaact tccattgacc
     5161 ataatatgag caacatccca gtaatcttcc acactgtcaa tcatatgggt acttattaat
     5221 agtgtgcatc cgctctcttt taattcaaca aacattgcct taagtacttt tattgcatga
     5281 ggatcaagtc ctaccagagg ttcatcaaat ataattactt tcggtttatg gacaagtgca
     5341 cagcatatag ataacttctg ctgcatccct ttagataact cttttcctag ttttcctttt
     5401 ttatcccata attcaaatct ttccaaaagt tccttttcgt attcatcatc tctggtttta
     5461 tatgcacgag caataaactc tagatgctct tcaactgtca gaagatcata aactgcaggc
     5521 atctcaggga tatatcccaa aagctttttt gcgtctatag atttattatc ataaccgtct
     5581 attttaatat cacctttaaa tcgtaacaag ccggatatac atttaatgat tgttgtttta
     5641 cctgctccat taggaccaag aagaacagca atctgtccat cattaacatg aaatgatata
     5701 ttatcatttg caactgtttt accatatttt tttgttacat tcgaaatttc aagcattgta
     5761 atcccttttg taacctgaat tccgcaccaa attagcgtag acaagtttcg acagatttta
     5821 agatgtcgtc ggttgcattg aaggcggaaa gaatcgattt ttgttccttt gtaagagcct
     5881 ttgtgaatct tgctttgccg cctgttgtaa taacagtaat atttgtcagt tggttgaaaa
     5941 cttttttgag cgaggtcgac ttttcatcaa ggtgcttttt gagtttaccc atgatatagg
     6001 ttcttattac gagtgcgata aacagcacaa aagctttgcc attggtggtt tgctcgttat
     6061 gtgtacggat tcgagctcct tgcatatcaa ttttgagctg gtcgaacagc ttctctgcgg
     6121 catctttggc gcggtaatgg taaagtacgt cttcgtggct tatttcatta tcggtcgaaa
     6181 atatcaggaa aaaccctttg tactggcgca gcaggtcgac cttgtcgaca tcaacagcaa
     6241 aatcaaaacc gttgccgtct gcgtgtttgg taatcctgaa atattgtgta tagcgtttca
     6301 gcctactcgc agggtaacgt ttcagccctg aaagttcggc gctaagcgag cgtatgcgtt
     6361 cggacatttc attgcacagc tgggcatggt tcatcggatc gtaaaacagc attaatctgc
     6421 cgctcgtgcc gtagtattca aaatgcttgt caacacagta aatgtcctta tcggaaagct
     6481 tgttcgcata cttttcaatg ccttgggagt gagatttcag catttcgacc gcaatatcca
     6541 gatatgccgg gatgcctatg gtgaacgctt tactgtgagt gctgagattg cggatgcatt
     6601 cttcgctgat aaagcctccg tccactacaa actttatatc tttcaagcct accgctccag
     6661 cattttcaag aacatatgac agattcgtct tgtctgtgag gctgccgttg taacggttga
     6721 aataaagcgg gagcttgctg ctttcatcgc agaacatacc gatgttgaac tgtggaagat
     6781 cctcgccgtc gcgattgtaa ccatactcaa cgtccgtcat tgttcgcgaa tacgaagata
     6841 ttgaagtaac atcatagcag atattttcgc ccgtgcgccc tacggcgacc cattttttga
     6901 aaaattcatg ggtcttggac agagatatcg actcaaaaag tctgctggta ctttgtgaat
     6961 tcagagattt agagcagccg ggcaccaagg ttcgctcaag ccaatcgtct atggcatcca
     7021 tggagctgcc ctctcgaact atgtaagcgg ctgatatgat tatgtccatg gtctgacttc
     7081 cgaatacctt atttaggcag gctgtcaacc ccatgtcatt gcaacatttg agaaccaagt
     7141 atgagtaccc atagtcccat acagcagcgt caagcattcc tggagttatc ttaaatattt
     7201 cgtaatagtt gctgttgggc tccatcttgc cggtttcggc atcaatcttg ccgataagta
     7261 tagccctgtt tcgaggcttg ccttctgcgt tgcgaaagta atgcgtgtac ttgtagacgt
     7321 acttctcgcc cttggccccg gcggttttag tgtgaacacc gtcacctgat ggaacatcaa
     7381 tcaacgaata ttttgaaatg gacatctcaa acccccctat ttttctgtat acgctaatta
     7441 tagcgtatac agaagggaaa ttcaagaggt aaaaaaataa ataaaaaaag cctgcgaaag
     7501 gcttgcagca tggctatttt aaataggtat taggatcatt cttacgtttt cctcccgtct
     7561 acgctaaatt tttagggagt taaggttgta aagtaattga aaataattca tatatgctta
     7621 taccacaaaa tcaggtaatt gtaaattgct gtacaaaata tgctaattat tacattaaaa
     7681 ttaataaatt aaatcacaat tagtacaaag tatcatttac atcagaaaag aagcatttct
     7741 gcttctcttc atttaagtta agactagtta tttattaaat atctcatttg tctttgaaga
     7801 agcatatact gaggtaatca aatctactgc tctcatgcca tcttctagtg aaacaaaagg
     7861 ttctcttcct tctaaaagag cttgtgtaaa gtctgttagc tgctttatat gtcctgttgc
     7921 cttaattata tggcttgcag gagaatcacc catcgtaata tcatctgctg tgtaatcttc
     7981 ctcattttca atataaagcc tgcatattct gtcttcttca atcagtattg taccttttgt
     8041 tccattaatt tcaattgttt tattctgggc aggattcatt gaagtagatg ctgtaatagt
     8101 gcctaatgat ccatccttga attcaatcac tgctacagca ttatcttcaa cctctatttt
     8161 atgcgtaaga gtacggatat aaccatatat atttttagtt tccccaatca agaataataa
     8221 catgtcaata ccatggatgc cttgattcat catagctccg cctccatcaa gcgcccatgt
     8281 acctctccat tttcctgaat cataatattc ctgcgagcga taatatttca tattcagatt
     8341 ggtcataatg atttttccca gtctgcctga tgatataatc tgttttgctt ttcttatttc
     8401 ttcagaaaat ctcagctgag atataacact gccttttacc ttattatttt taaatgcctc
     8461 aactactctc tttgcgcttt ctgctgtaag agataatggt ttttccacta ctgcatgctt
     8521 tttatgctca gcagcactaa tagctatcga ggcatgatat ccgcttgggg tacagatagt
     8581 gacagcatct attttcttgc tttgtaaaag tagatcagta ctgtcaaaac ttttaatacc
     8641 aaatttctta gcaaagctta caggttcctc agctgaacat ccacttactc ctactagttg
     8701 cgctcctttt atctgctgta aagcttctgc atgagtattt gcaatacccc cacatccgat
     8761 tattccatac cttataactt cattactcat ttttcttcct ttttatcact atagaagaac
     8821 catctaccac tttcagttcc tctcttttca tctccagcaa gtaattcttt gtaatttagc
     8881 gctactttct tgaacgcatt tgcatacatt tcaatgtact cgggcatgta atgtttaaac
     8941 catggtattg aatatacctg aacctgttct gataccataa gttctttatc aagttctctg
     9001 acatccctgt ctgtaaaagc tattcttgct ggtttttcaa aatttaaccc gttataatcc
     9061 ttaaatatgt tatgcatatg taacgctttg tttgcacctg gtgatgctcc aaatccttct
     9121 tttctgactg catcgcaaaa acgttctaca gaaagacctt ccagttcttc tgggcaataa
     9181 aggccatgag gcgcatacca tcctgccatg ttagacccat ctgactcgtc aaccatatga
     9241 gctcttagtc ccggaactcc ttctaaaaga ctccaaaagt acttcattgc ttttctaatt
     9301 tctgctgttc tttcttcata atacttaagc tgtactcttg ccattgctga agacaattgg
     9361 tttactcttc ctttgacccc tcccaatggc atagatgcgt atttttgcag atattctgtt
     9421 gttatatact ttgaattatt ccgttcgtaa tgacagtatg ccatagctct gtcatacatt
     9481 tccttgtcat cagtggcaag catacccatt tcaccagccg ctagagactt tccgctcatt
     9541 atggacattg ctgcaatatg accgaaagtt cctaacattc tgcctttata cataccaccc
     9601 tgagcatgag atacgtcctc aataactaaa atattatgtt ttttagcaat ttccataata
     9661 gggtccatat ctgcaggatg tccaaggtaa tgcacaacca ttatcgcttt tgttcttggc
     9721 cctatacacc tttcaatatc attaggatca atacataaag aagtaggatg aatatctgcg
     9781 aaaactactg tggaattgag gttaaaagct tgtaaacatg atgcccagta tgttttacta
     9841 ggacagatga tctcatctcc tgctcccatc ttgcatgcat acatagctgc ctgaagagcc
     9901 attgttccat tgcagtaacc aattgcatat tttcttttct gccattgagc aaattctttc
     9961 tcaaatttct cagtaacatc aagaccactc atagctcctc ttcttaatac ctcaagcact
    10021 gcttcctcat cttctttagt gactataggc cacttaaata gtgattcagg aattatcttt
    10081 tcatcaactg ctggttttcc gccaaatatt gcaagttcgc tcattataac gaccctccta
    10141 tttatatatg ttctatttta tattatatct tacaagtata tttttacttt gcattttcca
    10201 cttatttatt gactcttttc attcaattgt tttttctata ttgtccagga gtcagcccta
    10261 caacctgttt aaaagtccta ttgaaatggt ttatattatt aaaacctgat tcataacaaa
    10321 tagttataat cttttcatca gtattcttaa gcatctctga agcttttgca attcttaatc
    10381 ctgttatata ttctttgaat gtttttgaag taaggttctt aaaaagatac ccgaaaaaag
    10441 agggtgatag aagaaacatt tttgcaacag agtctatatt aagatcctga gtataatttt
    10501 cttcaatata tctaattgct ccttttattg catccttatg tctttcaaat atttgagcat
    10561 ttttactttt acttatattt ttagtaaatt ctctgcctac caggactagt agtttaagaa
    10621 gtagagaacg tataattaaa tcaaatcctg gttctctttt ttcatattcc tttaaagcct
    10681 cattaaagat gttctctgaa tcggtttgta tactcccaac caggtttagt ctgggtttaa
    10741 ccaaatcttc ttcaactaaa aaaggctcta tatatgcgaa atcccagaaa gcttctcctt
    10801 tgtctagatc tttaaaaaat tcatttataa acttcggatc aaactcaaac tcaactatct
    10861 ttgcatcagt tcctatgaca ggttcaatct gatgaggaac atttggagga attacaaaaa
    10921 tatctccttt tacaagctca attcttttat catgtataat atgtatggct ttcccgctat
    10981 aaacataatt tatctgtata cactcatgcg catgaagata caaagttcct tcttccggag
    11041 catatttttt taggtaaaac ataagatatc catctggatt gtcttctact ttgaaaacag
    11101 gtattttgtt tatcttattt ttatctgcca tttaatcaca tctgacaaca agaacaacat
    11161 tttccacatt tgctatgcta tcaattactt cttcatcttt tgaatcctta ggaattccaa
    11221 caactatgct aagccctata tttccatatg tcccgctctt tgcaggatga acttcaatat
    11281 tgtcaattaa atatcctgtt tcctcaattg tttttataac actattaata tttctgcttc
    11341 cagttacttc aagatataca tccatcttaa ttcttcttga accgaagttt cctaagaaag
    11401 tcataactac agctaaaatt aaaacacata ccaaagcagc ctcataaaaa cctgctccaa
    11461 ttgcaagtcc tactgctgca atcgtccata atcctgcagc tgtggtaagt cctttaacat
    11521 gtaaatgacc agtaacaaga attgttccga ctcctaaaaa acctattcca gacaaaacct
    11581 gcgctcctaa tctcatagga tcgctgtcaa aacccattgt ctgtgtcata aatattcctg
    11641 tcattgctgc catagtggag cctatacata caattatatg agtcctgaat cctgcagcac
    11701 gtccatgtct ttctctttgc atgccaatta aaccgcctgc aagcattgca agcaccagtc
    11761 tgattaccat tgttgcaggg ctaagttctc taagatactg tattatgtca ttcatttaat
    11821 cgttctcctt tagctgtttt ctaaatcgga tatccatccc tcatacgagg catcacttgg
    11881 cattctccag tctcctctgg gagacaatgc tacagagcct actttaggtc cattcgggct
    11941 gcaactcctc ttaaactgtt gtatgaagaa tctttttata aacacactta gccacttctt
    12001 tatctcttct tcagaataat cacctttaaa tgcttttttt gtaagcatta gtatcttttg
    12061 cggagtaaat ccccatcgca taaagtagta taggaagaaa tcatgaagtt catatggccc
    12121 tactatctgt tctgttttct gtactatctc tccatttaca gcaggcacaa gttcagggct
    12181 tattggtgta tcaagtatat cgtagagaac atctttgagt ttcccgcctg catcatctgc
    12241 tacatatcgt attatgtatt ttacaagagt tttaggtatt gacgtgttaa cattatacat
    12301 ggacatatga tctccgttat atgtgctcca tcctaatgcc tgctctgata aatcacccgt
    12361 tccgacaact aatccatttg tcatattgga taaatccatt aaaacctgag ttctttccct
    12421 ggcctgggca ttttcatatg tagtatctgt tgtcttacaa tcgtgcccta tatctgataa
    12481 atgactcttt actgacttag atatatcaat aaccttaaaa tctgtttcca gcagagcaca
    12541 aagtttcttt gcattatctt tagttctctt tgttgttcca aagcatggca ttgaaacaca
    12601 taatatgcct ttagtatccc tgcccatttc aatatatgct ctgtgcgtta ccagtaaagc
    12661 cagtgttgaa tccagtcctc cggatatacc aataactgat gtgtccagtt ttgaatgaat
    12721 cagtcttttt ttcagtcctg cagactgtat gttgaatatc tgattacatc tttccattag
    12781 cactgatgtg tctttaggaa tgaaaggatg cttttcaatc agcctgtcta tttttgtttc
    12841 tcttagatcc atattgaatg ggatgtacat gtagcctctt tcggtccagt cttttcttac
    12901 agacacctgg tttaccctgt catgtgctaa tcttgataaa tcgatatccg cttctaaata
    12961 ctcttttcca aagggaggtt cttctgccag tattactccg ttttctgcta tcatcttatg
    13021 tcctgaaaag actatatcgg ttgttgattc tgaataactt gaattgctgt atatgtaagc
    13081 actgaagcat tttcctgatt gtgctttcac tagagttttt ctgtattctg atttacctaa
    13141 gatctcatta cttgctgata agttagctat tatcatagct ccattcattg catgttctga
    13201 tgatggacta acaggaacaa ataggtcttc acaaatttca acagctaatg tgaattcttc
    13261 catctgcaaa ttttgaaaaa taagttttgt tccaaaagga atctgcttac cttctaagtc
    13321 aagatatatc gtgtttttcg gtgcttcact aaatctgcgc gcttcgtaaa actcattgta
    13381 attggggata tatgtctttg gcactattcc taaaatttcc cctttacata atactgctgc
    13441 tgtgttgtac aaaaatccat ctacttccaa aggaagccca actatactca gcatatctat
    13501 accagaagac ttgtctgcca attcaatcaa acttattctt gcctggtcta aaagcagttt
    13561 ctgagcaaaa aggtcgtcgc aggtgtatcc tgttatattc agttcaggaa agactaaaat
    13621 gttcacacct cttttatagg cataatccat ggttttaata atattgtcac agttatactt
    13681 acaatccgct actttaagca tagggcttgc agcagctgct ttaataaatc cataatgcat
    13741 attaaatccc tcttttcata tataaattat acactgttta taaataaatt aacatatttg
    13801 agatagaata ccctataaat cgtcaggaat cagagtctta ttgaacttta gaaacagtcc
    13861 gccagcaatt atctgagcta ctcctgttac agcaagcagt attggaactc ctgccatttc
    13921 aaagttgaat atatttagaa catttccatc catacctgat aggaaaaatg ttccaagcat
    13981 catacttaag aatatcgatg tgtgaacaaa aatattatag aaagagatat agtttgtctg
    14041 gtcttgtttg ggaaggttca catacagcat agaggctaga acagtgtttg ctgcaactcc
    14101 gagaacatgt tgtaataatc ttataccaaa ccacaaccat gtatagttct ctggtgtaac
    14161 aaaagcatac ataagatatg taggtccctg aagaaacatt acaaaagcga aagttttaaa
    14221 ccatgagtgt tttttaagaa actttttcca catctttccg aaaaagataa agaacatgaa
    14281 gtatactgcg ttaatgaggt ttatttctga atagcttatt cccacatcct gcagtagaaa
    14341 tatatttatg gatgcattag taagagaact tcctatgtaa taaaaagcta ccagaagtac
    14401 agttaaaaga aactttttat tttgaaaagg taatgtgaat atatcactta ttttaatctt
    14461 gcttttactt cttacatatt catattcttt tggaatggct aatgccacaa catgaagaat
    14521 ccctacaact aaagcagcat atcgaaagcc tgttattatt acaagctgtt gaggagaacc
    14581 tgcaaaagag tctgttatta aagaaaggac taaaacaatt ccctgagtca gaaaactgtt
    14641 tatacctgat gacaaagtaa aatagtcagc tcttatttca ttaggtaaaa aagtagaatg
    14701 ccatgcagaa taacctggat cgattatcgc cgatatagca gaagacatag taacaagcaa
    14761 agtaaaccca attacccttc cagcttctgt tgtgacgata tttggaagca gtgttatacc
    14821 cagtatattt attatgttag ctattatgct taaagtggta agaatagcct ttcttctacg
    14881 gaatctctct agaataaatg gagaaaacat agacaataat ctagtaattg taggaacgaa
    14941 tgtaataata cctatattta caatgttaat gccataactt agaagaaaac cagtatagaa
    15001 tattcctccg cttaattgtg aagctaaaga tgcaagtacc gatgaggcta gaaagcaact
    15061 tctccctttt gctttttcat catgaagatt aaaggtcctc acaaaggctg aattacgcca
    15121 ttttatcttt atcgcatgca gatatgcatt cataaaaaat actttaatct cctttacgtc
    15181 ctgagaattt tacaatacca cgattgttaa gtcaacacaa tacagggtaa acattatgaa
    15241 ctttttgtta caaattgctt gttgcgatat atctttggtt atgataaatt ataactataa
    15301 ttaatagaac atgatattga ttgcctttcc cgtataaaac aaggccaagg cgtcatcaaa
    15361 aggcattctt atcaagaaat ttagcatgct tttaataata tttttaatta attgcattaa
    15421 atcaaaagga ggaatcatat ggaagagaaa aaacccaaga aaaagaaact aacaactgat
    15481 gttggtgctc cggtagcaaa taaccgtgac gtgctgactg ctggaagaag aggaccaatg
    15541 ctcttacaag atgtatggta cctcgaaaaa cttgcacact ttgacagaga ggttattccg
    15601 gaaagaagga tgcatgcaaa aggctcagga gcctttggag tatttacagt tactcatgat
    15661 ataaccagat atacaaaagc aaagatattc tcagaaatag gcaagaaaac ggatatgttt
    15721 gtgcgttttt caactgttgc aggagaaaga ggagcagctg atgcagaaag agatattcgc
    15781 ggatttgcaa tgaaattcta tacagaagaa ggtaactggg atcttgtagg aaacaacacg
    15841 cctgtattct tctttcggga cccgcttaaa ttcccagatt tgaatcatgc agtgaaaaga
    15901 gatccaagaa caaacatgag aagccccact aataactggg atttttggtc atcaatacct
    15961 gaagccctgc accaggtcac aattactatg agcgataggg gtataccgta ttcatatagg
    16021 cacatgaatg gatatggaag ccatacattc tcaatgataa atgataagaa cgaaatagtc
    16081 tgggtaaaat tcacacttaa aacgcaacag ggcataaaga atcttacaga ccaggaagca
    16141 gaagcagttg tagctaaaga cagagaaagt catcaaagag atctttatga agcaattgaa
    16201 agaaaagagt atccacgctg gactatgtat atacaggtaa tgactcagca gcaggcgaaa
    16261 aaaatgaaga ataacccatt tgatcttaca aaggtctggt ttaaaaagga ttatcctctt
    16321 atagaagtag gatatttcga attaaacaga aacccagata attatttcgc tcaagttgag
    16381 caggcagcat ttaatcctgc aaatgtcgtt ccaggtatcg gtttttctcc agacagaatg
    16441 ttacaaggac gtcttttctc atatggagat gctcaaagat atagactggg agtaaattac
    16501 aacaatattc cggtaaatgc gccaaaatgc ccttatcata gttttcatcg tgacggactc
    16561 atgagaacag atgacaatca gggaagcctt cttcactatt atcccaactc ttttggagaa
    16621 tgggaagaca ataaagagca tgaagaaccg ccccttgata tcgacggtga tgcattcaga
    16681 tgggattatc atgaggacga tgatgattat tacacacaac ctggcaaact tttcaattta
    16741 atgacaaaag accagcagca ggttctgttt gagaacactg caaggaatat ggatacagta
    16801 cctcagttta taaagatacg tcatataaaa aattgcgcta aagctgatga agcatatgga
    16861 agaggagtcg caaaggcttg tggaatagat tatgaaagta ttgaataaat agcagttata
    16921 aagataagga agatttatgg aaagagtatt taaatacgat attatgcagg aaataaaaga
    16981 cagatggtcg ccaagagcat attccgaaga aatgatatca aaagacgatt tgtatgcaat
    17041 tttagaagca gcaagctttg cgccatcctg ctttaacgag cagccgtggc gctttgttct
    17101 tgctgatgat gaacaatcta ttaaaaaaat gagaagcatc ctatattccc agaatttaaa
    17161 atgggcagag aaagcacccg ttttgcttct catagcagca aagaaaacat tttctttaga
    17221 tggtgaacaa aacttctggc acatgttcga tacaggaaca gcctgggggt ttttgtcttt
    17281 ggaagctcaa aaaagaggga taataaccca tgcaatggga ggtttcagca aaaaaaaagc
    17341 taggaaagaa ttcaatatac ctgatgattt ggaaataatt acagttgtag ctatcggaag
    17401 atatggagat ataaaccaac tagaagatga actaagagaa agagaaaaac cagaaaccag
    17461 acaggatata tccagactta taattaacaa ggagccatat catgattaga aagcctttac
    17521 aaacaataac cggaataaat acctctgatg gtgccggtgt taagcttgtc agagtaatcg
    17581 ggcacagaaa tgtttacgat tttgatcctt tcttaatgct tgatgcattt gattcaaatg
    17641 accctaaaga ttatataaag ggttttccat ggcatcctca tagaggtatt gaaactgtaa
    17701 cctatttaat ctcaggagat attcagcatg gagacagttt aggcaatgaa ggccgtatac
    17761 tcgatgggtg ctgtcagtgg atgacagcag gaagcggaat aatgcatcag gaaatgcctc
    17821 agccaagcag aaaaatgtta ggtgttcagt tatgggttaa tctacctgca aaaaataaga
    17881 tgacttcacc taaataccat gatattgaag caaaagatgt tcctttaata aaaaaagatg
    17941 attacgaggt cagagtaatc tctggttcat tcgataatgt caaaagcaaa gataatgatg
    18001 aatttgttaa aactacattt cttgatgttc ttctaaagcc tgataaacct tttgagattg
    18061 aaacagctcc agacagtact ctttttttgt atattgttta tggtgatggt tatttcgctg
    18121 accttgatga attgttcagt tcaaaaagag cagtcttatt tgataaagga gagatatttc
    18181 acgcaaaagc aggaaaagag ggaattcgat ttcttttatt taatgcaaaa ccgcttaaag
    18241 aacctatttc atggggtggt ccaattgtaa tgaataccaa ggaagaactc caaaaggcct
    18301 ttgatgacct ttcaaatggt acttttataa agaaaaactg attaatgaat acattaataa
    18361 atatacatta aggtatatat tttgtataat tggcatgtga ggaattcttt cggaattttg
    18421 aaagattaag gaattcaaaa tgcaagagca gttttatgaa aatcttataa gggaaattcc
    18481 cgtagcttat gcatatcata aaatgattta tgatgaatcc ggcaagccta ttgattatga
    18541 atttttagat gtgaatgaag cttttgaaaa aatgacttca ttatcttctt cacaaattat
    18601 cggaaaaaga gtaactgagg taattcctac aattattgag gattctttca attggataga
    18661 agagtatgga aaaatagcac aaagtcagat aagcaatgag tttgagactt ttgctgatac
    18721 tcttcaaaga tattacagag tatttgtatc ttctcctaaa aagggatatt ttacgactat
    18781 atttttagat ataacaaaaa caattataaa gacgcaagag caacttgctc tgttaacaat
    18841 gcttaatgat attttcattg aaatggatga gaactataag attataaatg ttttagctcc
    18901 tgataatata ctggacagac ccaaaaatga acttatcggc agatatatgt ataaaatatc
    18961 accaaccgaa ttctttgata aaataaagca ttactcagat ttagcaaaaa agaaaaaaac
    19021 aaaggttact tttgaatatt cattttctgt taaaaataag gttagatggt atcgaagtga
    19081 ctgcttatac actaagatac tcaatgaaga aagaattgtc ttctttatat ctgacataac
    19141 tgaaaaacat gatttagaaa acaagctaac acagagcgaa tcacttttca gagcactatt
    19201 tgagcaggcc ccaataggtg tagctctagt taaaaatttt aaattcatgt caaacatcaa
    19261 tgataaattt gttgaaataa tgggaatgac aagaaaacag ctcattagca cagactggaa
    19321 agatataacc catcctgatg atcttgaaga agatttgaaa tatttcaatt tattcaaggc
    19381 cggcaagata ccctcgtatt ccatggttaa aagatttata agaagtgaca gaagtgtcgt
    19441 atgggtgtat atggtaataa cttatttaat gctggatgaa tatgaagaaa gaaaagacac
    19501 tcatgtgtgt atgataaatg atattactca gataatggaa atgaatgatg ctttaaaaga
    19561 aagtgaaaga agcaagtccg tcatgctctc tcatcttcca ggtattgcct atcgctgtgc
    19621 aatagataaa gactggacaa tgcatttcat ctctgacgga tgctatgaac tgacaggata
    19681 tcctcctgat gcagtattaa acaacaaaga aatctctttt aatgaaatta ttgctcctga
    19741 atatagagac atggtttata agcaggcaga tgaagttgct aaaaataaga cctatttcaa
    19801 agcagaatat gaaataatcg cttcagatgg aagtagaaaa tgggtatatg atcagagtca
    19861 gtttttttat gataataatg ataatcctat tatcatggaa ggcctaataa tagatattac
    19921 tgagaacaaa aaaagagagc tgcatatcgc ttatttgaat tctcatgata tgcttacagg
    19981 actatataac agaatacatt tcatgagcga aattcaaagg ttagataaca aagaccattt
    20041 acctctttca ataatcatag gcgatataaa tggtgtaaga ttagttaaca atgcctttgg
    20101 cacaaaaaga ggtgaccgtc ttttactaag aatagcaaag ataattaaaa gcaattgcag
    20161 acctgacgat attgttgcaa gaataggcgg agatgatttt gcagtactta tgcctaatac
    20221 taataatgcg actgcagaag cagttactga gaaaataaga atggaatgcg aaaaactggc
    20281 taataagcct cttaaaacac ctgtctttac aaatatttca ttaggttttg gcacaaaaat
    20341 caaaaactcc gaagatataa accaaatatt cagagaagct gaagatttaa tgtttaaaag
    20401 aaaattatta gatagaaaaa gttctcatag cgatattcta gctgcaatac tagcctcatt
    20461 attcgcaaaa agtgaagaaa ctgaagaaca tgctcaaaga ctttatgaat tatcaaagaa
    20521 aatcggtaat cattttcatc ttaattcaag agatttggat ctgctccagc tttttacact
    20581 attacatgac gtaggtaaaa taggaattga tgacagcgta cttaaaaagc ctggaaaact
    20641 tacagacaaa gaatggatag aaatgaaaaa gcaccccgag ataggttata aaatagcaat
    20701 atcttctcct gaactggaac ctatagcaga tcttatctac acacatcatg aacatttcaa
    20761 tggaaagggt taccctcagg gattgaaggg agaagaaatt cctcttttat caagaatctt
    20821 atcagtagct gatgcttttg atgccataac tcatgacaga gtatatagaa aagcagcatc
    20881 tattgaagag gcaataagtg aaataaataa atattcagga acacaatttg atccaaaagt
    20941 agtagaagca ttgaataata tatacatata gatggacaac atttagttta ttggatataa
    21001 taaaagtcta ataatacgaa gtattaagaa gggagatata aaatgaaaaa tgaacatcaa
    21061 aaattcttta tttgcagaca ctgtggaaac atcataggta tgatttttaa ttcaggagta
    21121 ccaatagtat gctgtggaga agagatggaa gaattagttg ctaatactac agatggtgcg
    21181 catgaaaagc atgttccagt agttaaaaaa gaaggcaacc tggttcatgt tgaagttggt
    21241 tcagtacctc atcctatgtt aaaagaacac tatataatgt ggatatatct tgaaacagaa
    21301 aaaggtgggc aacgtaaatc tcttatgcct ggagaaaaac ctgaagtaac ctttgcagtt
    21361 acagatgatg ataaagctat tgcagcatat gaatactgta atcttcacgg attatggaaa
    21421 agtgacgtaa aatagagtaa aatagaaaac gttaaatatt tatgaggagg taatttcttg
    21481 tgaaaaaata tgtgtgcaca gtgtgcggat atatctatga tgaagccgat ggagatccag
    21541 ataatggtgt agagccagga acaaaatggg aagatgtacc agaagattgg gtttgcccgt
    21601 tatgcggagc ttctaaagaa gagttcgaag aggagtaaga tatattggag tttaatccca
    21661 aagaagcggc tattgcatag ccgttttttt gttgacaaat caaattgcac tgtgataata
    21721 taattaaaac ttaggagaca gaaatgaaga acttgttttt tacagctgaa tattattact
    21781 tttactttag ctttagaggt aaaaaggtat ttgctgcaaa aaacaataac cgataaaaag
    21841 acttaaaagg ttaacaaagg tttcgcagtg aatacccaca gtgaaacctt tttttgtata
    21901 aagaaaaggg ggtcatatca tgatagctgt tttaaagcca aatgcaccgc aagcacaaat
    21961 tgaaagtctt attacctggt ttaaagacca gggtctaaat gttcatgttt cagtaggtga
    22021 ttatcagacg attttaggac ttataggaga caccagcaaa atcgatgttg aattactgga
    22081 agtattggat attgttgaat ctgtaaaacg aattagcgaa ccatttaaga atgcaaatcg
    22141 caaatttcat cctgatgata ctataataaa agtaaaagat gtcactatag gtggtggaaa
    22201 ctttgttatg atagcaggtc cctgttctgt tgaatcagaa agtcaggttc ttgaaatagc
    22261 acaaaaagta caagctgcag gagccaatat gttaagagga ggggcattca aaccccgtac
    22321 ttccccatat gattttcaag ggatgaaagc tgagggaatt gaacttttac taaaagccaa
    22381 gcaaagaacg ggtatgccga ttgtttcaga aataatgaat gctaatcata ttcctctttt
    22441 tgaggatgta gatattattc aagtcggagc aaggaatatg cagaacttcg agctgcttaa
    22501 agaactgggt aagactaata agacgatttt actgaaaaga ggcttagcca acaacttaaa
    22561 ggaactctta atgagtgcag aatatataat ggctggagga aacgagaata ttattctttg
    22621 cgaaagagga attcgtactt ttgaaactta taccagaaat actattgatt tatctgctat
    22681 accaatgctt aaggaattaa gccatcttcc tgttatagtt gatccaagtc atgcaactgg
    22741 aatggcaaaa cttgtaagac ctatggcatt agctgcagct gcagcaggag ctgacggact
    22801 gatgattgaa gttcataatg atccattaca tgcgttatgc gatggaatgc agtctcttac
    22861 tcctgagcag tatgctgaag tagctgataa agtacgtaag atactggagg ttatcaagtg
    22921 aaaataggaa tagtagggct tggactgatt ggaggttctt tagcaaaaac atataaaagc
    22981 aactctgata atgagatata cgggtatgat acaaataaac ctgtccttga tatagctaaa
    23041 atgtcaggtg ttatagatgg agttttagat gtgtcgaaaa taagtatgtg tgactgtata
    23101 ttcatagctc tttatcctaa agcaactata gactatttag aaaagaatgc acaatatata
    23161 tctaaaaacg caattgtaat agatacctgt ggaactaaaa gaaaaatttg tgaaactggt
    23221 ttctcaatag caagacaaca tggttttgtc tttgcaggtg gacatcccat ggcaggaaag
    23281 cagaattctg gatataagta cagcacatca acacttttca aagatgcata catgattgtc
    23341 gttcctgatt ctactgataa cattagagct cttgaaagaa taaaaaaaat acttgagcct
    23401 ttaagtttta aacatataac tataacgact gcgcagaagc atgacaaaat gatagctttt
    23461 acatcacaga tggcgcatat tgtatctaac gcatacataa aaagccctac tgcaaaggct
    23521 cataaaggtt tttctgcagg aagttataaa gaccttacaa gagtagcata tttgaacgaa
    23581 aacatgtgga ccgagctttt tcttgaaaac aaagataacc ttgcaaatga gctgaatctt
    23641 ttaattgaac aattatatga atacaaaaaa gctattgaaa ataatgatga tatgaaatta
    23701 aaagaattgt tagaacaggg aaaagaatgc aaggaggagg ttgatggccg atgaaaaggg
    23761 ttattgttaa aagttcaaag aagtattcaa taataattgg aaaaggattg cttgaaaaca
    23821 tttctgacca tatcctgcct atattaaaag gtaataatgt gtatatcatt actgatgata
    23881 ttgttgaaag cttatacatt tctcaattaa taaaaagtct ccaaaagaat aatcttgtag
    23941 ttcataaata tgtatttcca aatggggaga aatcaaaaaa tgcaaaaaac tatatagata
    24001 tactaaatga catggcacaa aaagggattt caagatcaga taccgtaatt gcgtttggcg
    24061 gaggtgttcc tggtgatctt ggtgggtttg ttgctgcaac atatcttaga ggaattaaac
    24121 ttatacagat acctacaact ttgttatcag cagttgactc ttctgttgga ggaaaaacag
    24181 caattgatct tgataaaggt aaaaacatgg ctggtgtttt ttatcagcct gatttggttg
    24241 tctgtgattt atcttcattg aacacattat ctgatgagac tctgaaagat ggatgtgctg
    24301 aagtaattaa atatggaata ttgtcaggag gtttgctctt taatcaatta aaaaaaccta
    24361 taatgaaaca tattgagagt atagtatatc agtgtgtaaa atataaaaga gatattgttc
    24421 aaaaagatga acatgaccag tatttacgtc agttgttaaa cctcggtcat actataggtc
    24481 atgcaattga actattaagc ggatataaag ttacacatgg gcaggctgta gctaaaggaa
    24541 tttctgtaat atcaaaaata tcattcaacc tgagcttatg tgcaaaacat actgtagatg
    24601 aaatagataa tattttaact ggatatggtt ttgacttaaa tcctatgtat agcccctcag
    24661 atttggcttc tgcaattatt atggataaaa aaagatgcaa tgattcaata aatcttatat
    24721 tagtagaaga tattggaaag tgtattataa gaaagaccat gatgaatgaa cttgaggaca
    24781 tgataaaaaa agctataacg aaggtaccat tatgaatatt tccttgacaa taaataatcc
    24841 tactggtgaa ataaacgcaa tagcatctaa atcatatgct cacagacact tgatatgttc
    24901 agcattgtca gacaagccta actatttaaa aattaaccag tcttctcagg atattgaagc
    24961 aacaatcaac tgcctaaatg cccttggagc aactattgta aaaaacgata atgggattac
    25021 gatttctcca ataagtaaga taataaaaaa tgcttgcctt gattgtaatg aatccggttc
    25081 aactttcaga tttatgcttc ctatttcttg tgctttaaat tctgaatcag tctttcaaat
    25141 gaaaccagga cttgcaagaa gacctattac aaatttgtat ttacagctta aaagtaaagg
    25201 atgcatctta tcagaagaag gttctgtacc tttttatgca aatggccaat tgcaaggcgg
    25261 tgatttctcc ctccctggaa atatttcttc ccaattcatt acaggtcttt tgatggcttt
    25321 acctttatta aatttaaaaa gcacaataac attaacatcg gaccttgagt cttccggtta
    25381 tgtagatatt acaattgatg tattaaagaa ctatggtgtt aatattgaga agaaattcaa
    25441 taaatttata attcctgaaa accaaaaata tataagtcct ggtaatgcag atattgaaaa
    25501 agactggtca aatgcagctt tcttcttatg cgcaggcgca ttaacagata aaggaatcac
    25561 tgtaaccggt ctaaatcgaa actcccatca gaaagatatg tatatactta atgttctcaa
    25621 agatatcgga gctgatatct catttagtga aaataaaata actgtaaaaa agaataaact
    25681 gaattcattt gagttagatg ctaaagaaat tcccgatatt atacctgtat taagtctagt
    25741 agcaagttta tcaaaaggta cttctagaat atataatgca aaaagactta gaataaaaga
    25801 aagtgacaga ctttcttcca tttatgatat gctaaagaag attggtgcac aaatagaaca
    25861 aaacgatgat actcttgtta taactggtaa acccatgtta aagggcggta tagttgattc
    25921 ctttaacgat cacagaatag cgatgactgc agcaatagct tctatttcat gtgaaaatcc
    25981 agtcataatt aaagatgctc aggcgataaa taaatcttat ccatcctttc tagatgactt
    26041 tataaaaatc ggaggaaagg ttgaaacggt ttcaggaggt aatgatgtct tcagtattcg
    26101 gtaacaatct aaaagtatct atctttggac aatcacattc aaaggcaata ggggtatgta
    26161 ttgacaacct cccgccaggt tttaagattg acatggataa aattaatgct tttatgaaaa
    26221 gaagaagtgc tcaaaacaaa gcatatgcta caaaaagaaa tgagccagac gaaattgaat
    26281 ttgtatccgg tcttgtaaat ggcatgactg ccgcagctcc attatgtgct ctcatattta
    26341 ataaggactt taaacctaaa gattatgaaa atctaaaaaa catacccaga ccttcccatg
    26401 cagactatac ttcatatgta aaatacaaag gatttcagga ccctacaggc ggaggtcatt
    26461 tttccggaag gattactgct gctttatgta tagcaggagc aatctgcaaa cagattcttg
    26521 aaagtgaagg aatgactatt aatgctcata tatataagct tcatggaatt tctgatcagc
    26581 cttttgactc ttcaggtaac aataaaaaac agataacaca tttgtatagt aatgattttc
    26641 cagtaattga tatagataaa ggtaatctta tgcttgatga gataaaaaag gcatcagata
    26701 aatctgattc gataggcgga ataattgaat gcattataga agacgtacct gctggtatag
    26761 gcgatcccat ttttgacggt atagagaata atatctcaaa agcaattttt agtatacctg
    26821 cgattaaagg aattgaattt ggtaatgggt ttttatgtgc tgacctcaat ggatctgata
    26881 ataatgacgc cttttgtatt aaagacggaa aagttaagac aaagaccaat aatcatggtg
    26941 gggcattagg aggaataagt tcatcaatgc ctataatatt tcgtgtagct gttaaaccta
    27001 caccatcaat aggcattgaa caagacagca ttaatcttga ttctatgaaa gaagagaaat
    27061 taaatataaa tggcagacat gacccctgca ttgtaccaag agctgtaccc tgtgttgaag
    27121 cagcagcttc gatagcacta ttagatatgt atatgtcata taaaatatgg aggtagtgaa
    27181 atgacaattg ataaatacag agatgaaata acagcaatag atgataagct attggagctg
    27241 tttcataaaa gaatgcgtat tgccgagaaa atagcacaat ataaaaagga aaacaatatt
    27301 cccattttaa acacttctgt tgaaagagct aagcttgcag aactttcaga aaaagctgat
    27361 gatgatatgc ttttctactc tagaatacta ttcaatacgc tttttgaaat gagcagatca
    27421 tatcaggaaa aattaaatca ccctaaatcg gttctgatgc aaaaaattga atcagctatt
    27481 gaaaacacaa atagactttt cccatcaact ggcaatattg catgtcaggg ggttgaagga
    27541 gcatattcac aattagcttg cgacaaactg tttgattcac ctaacataat gtattttact
    27601 acttttgaag gagttttttc cgctatagac aagaatttgt gtaaatatgg agttcttcct
    27661 ctcgagaaca gcactgcagg ttctgtaaat aaagtatatg accttatgat gagatacaaa
    27721 tttaatatca taagaagcgt aaggctgaag atagatcata atctgctggc aaaaaaaggt
    27781 gtaaagaaaa atgaaataaa ggagatatac tcacatgagc aggccatcag ccagtgttct
    27841 gagtttatta aacagttagg caatgtgaag attaatgtat gtgagaatac tgcaattgcg
    27901 gcaatgatgg tagctaatag cgaaagattt gatattgcag ctatatcatc ccgtccatgt
    27961 gctgaacttt atggtctaaa ctgtgtaagt gaatcggttc aggataaagg aaacaattat
    28021 actaaattca tctgtatatc aaaagacatt gaaatatatc ctggtgcaga taaaacaagt
    28081 attatgatag ttttacctca taaaccaggt tctctttaca gagtgctcgc aagattctat
    28141 atactaggaa taaatctcgt gaaattggaa agccgtccaa tacccgacag agactttgaa
    28201 ttcatgttct actttgatat cgagacaccg gtatattctg aacagtttac tcagcttata
    28261 aatgaacttg acgaaatgag tgaagacttt agatacttag gtagttattt ggagataata
    28321 tgaaaaaata tggactgctt ggcagaaaat taggacatag ttactcgcca ataattcatt
    28381 catttttagg aaaatatgaa tatgctcttt acgaaagaga acctgaacag ttaaatgaat
    28441 ttttttcaga tagcacagtt tcaggattta atgtaacaat tccatataag aaggatgcat
    28501 tgaaatactg taaaattatt acttcaattg ctgaaagaac taaatgcgtt aacactgtaa
    28561 taagaaaaaa tggaaattat cttcttggag aaaacactga ttattatggt tttatgtata
    28621 tgataaagaa aagcaaaata gccattgaag gtaaaaaagt tctgctttta ggagatggcg
    28681 gttcagctgc tacaatcaaa gtagtaattg aggatttaaa tgctaaaaac cttgttacca
    28741 tatccagaag agggccggtt aattatgaaa acatacatct tcattacgat agtgatatta
    28801 taataaactc aactccagtg ggaatgtttc ccaataatgg tgaagccgtt atagatatat
    28861 caaaatttaa aaattgtatt gctctttttg acctgatata caacccagct cagacaaaac
    28921 tgatgtatga cgcaaaacag ttaggcatac cttcatataa cggactaaca atgctagtcg
    28981 cccaggctaa gaaagcatct gaactattta ctggctcagt tattgatgat tctgttattg
    29041 agaatattat agataaagta tcaaaaaaat ctatgaatat tgtaatcatt ggaatgcctg
    29101 gaagtggaaa gagtacagtt ggcagagcat tagctaaatt aactgacagg tgctttattg
    29161 acactgatga tgaggtatca agaacatacc ataaggatac agcagaaata attgttaatg
    29221 aaggcgaatc cgtctttaga gatcttgaaa cacagatatt agaaaatgta acgaagaaaa
    29281 gcaactgtgt aatatccaca ggaggcggta ttgtcacaag agaaagaaac aaatatctca
    29341 ttcaacaaaa cagtattata gtttatctaa aaagagatat atcactatta tcaacttata
    29401 accgacctct atcacaaata aatgacttaa aaaaactctt tgaatccaga gtagataagt
    29461 atgaaggctg gagcgattat caggtagatt gcgatgatat ttcaaacaca catattaaaa
    29521 taaaggagat gttaggattg tgaaactttt agtaatcaat ggtccgaact taaatatgct
    29581 cggaataaga gaacctgctg tttatggatc ttcaacatac aatgatcttg ttgattatat
    29641 taatactgta tgtagcaaaa acgatattca gtgcgagata tatcaatcaa atcatgaagg
    29701 agcaataata gacaagatac aaagcgcata caaagtgttt gacggaattg taataaatcc
    29761 tgcagcatac actcatacaa gcttagcaat tgctgatgca attaaatctg ttggaattcc
    29821 tactatagaa gttcatataa gtgatatatc aaaaagagaa agtttcagac agttttcata
    29881 tacaagtatg gtatgcataa aaactattat gggaaaaggt tttctaggtt atgaagaagc
    29941 aataactgaa ctgaaaaata ttatataaat atcctttatt gtaaatattc aatatcacta
    30001 agttccccat ttttgataat acgaacgtca tttccatgtt tgaccagttt taaatatatg
    30061 cgactatcca acatatacgc atttgtatgt ttaacatata agacaacata acttaattta
    30121 caaaagcaat aaggccaact attggctgca aaaagccaca aacaaaatta ctgtttgcgg
    30181 cttctgttta tctggcttat tattttttac ttatccttct gttcaaccat tattttcttt
    30241 ggtttaataa ggcctaaagc ttttgatatc tccattacaa acagaggtac aaatgatagt
    30301 gccaatccta ttaaatatgc atttaagtca agagatataa gctggaatgc tgttgacaaa
    30361 ggagtaaaca atacaagtgc aataagagct gcagatatta aacaagcaat attcagcctc
    30421 ttatttgtga agaatccgat tttgaataac gaatgttctg atctcatgtt aaaggactga
    30481 acaacctgag ataatgaaag aacaaagaag gccattgtct gtcccgcttc aagtgtacct
    30541 ataacatttc ttccataaat gaaaccgcca attgttattg cagcaatcat acatccttgc
    30601 agaataatcc ttataccata accatgagcg aatatacctt catttcttgg ttttggtttt
    30661 tgttccataa catcactttc tacatcttcc atacctaaag aaatggctgg aagactgtca
    30721 gtgacaagat taatccataa tagttgcatt gataaaagtg gagtttctct ccataaaatc
    30781 attgcaaaga aaactgtaag tatttcacct atatttgttc caagaaggaa tccgaccact
    30841 ttttttatgt tcgcataaat tcctctgcct tctcgtactg catcaactat agttgcaaaa
    30901 ttgtcgtcgg taagagtcat atcggctgct ccttttgcaa catcagttcc agttataccc
    30961 attgcacagc caatatctgc tgcttttaga gccggagcat catttacacc atctcctgtc
    31021 attgaaacta cttcacccag cctttgccat gcccttacta tccttatctt gttctcaggg
    31081 gaaactctgg cgtaaactga tatgtttctt actctttttt caaactctga atcagtcata
    31141 ttatctaatt caatacctgt aattgcctca tcattctccc ttaatatgcc taactcggta
    31201 gcaattgctg acgcagttat aacatgatct cctgttatca taaccggttt tatacctgct
    31261 tttctgcaaa cagcaacagc atctttagct tccggtctag gtgggtctat cattcctaca
    31321 agacccatga atataaggtc tttttctata tcttcaggct tgagttcttt tggaatactg
    31381 tcaatatact tataagctac agctattact ctgagagcat ttttgctcat tgtgtagttt
    31441 atatctttag ccttttcctt atctccttct atgcatcttt ctatcagtac atcaacagct
    31501 cctttaacta ttacaactgt tttatcatcc attacattaa tagatgtcat tagctttctg
    31561 tcagaatcaa aaggaatctc tgcttttctt ggataagacc tgttaagatc ctctttttcc
    31621 aaaccatttc tatgtgctgc atatactatt gctgtttctg ttggatcccc tatatgcttt
    31681 tctgcgtctt ttgtgaatac aaccgacccg ttactgcata gtgttccaaa tttaagaagt
    31741 ttttttatgt caggagagtt atttactgtt atcttttctg ttttttccat accatcatag
    31801 tatgcttcaa cgagagtcat tctattttgt gttagggtac cagtcttgtc agaacatatt
    31861 attgaagcac tacctaaggt ttctactgca ggcaaatttc tgataatggc gttcttctta
    31921 accattctct gaacaccaat tgataataca atagtaacta tagccggtag tccttcaggt
    31981 attgctgata ctgcaagtgc tacagctatc ataaatattt ccattacagg cataccgtcc
    32041 ataataccta caataaagat aactgcacat gcagccaagg caacaattcc aagatatttg
    32101 cctaatgatg caagcttttt ctgtaaaggc gtctgcgtac tttcttcgct ttctaaaaga
    32161 tttgcaacac gtcccatctc agtatccata ccagtagttg taacaacagc taacgcagta
    32221 ccgtaagtta tggagcatcc tgtaaaaacc atattagttc tgtctccaag cggtgcattt
    32281 tctgcaactt cttcatctgc atctttctct gatggaactg actctcctgt cagagctgct
    32341 tcttctgatt ttaggctaga ggagcggata atccttgcgt cagccggtat aaaatcacca
    32401 gcttcaagcc ttataatatc tccaggaaca agtaatgatg cttcaataac ctgttcttcc
    32461 ccatctctta taacccttgc attaggagag gacatatttt ttaaagcttc caaagctttt
    32521 tcagctttgc tctcctgcaa tactcccata attgcattca atattacaat aagcattata
    32581 agaacaggtt caaggaattc ttttgcttct ttttcatata aagctacaat aaatgaaatt
    32641 attgcagcaa ttatgagaat aataatcatc acatccttga attgatctaa aaacctttgt
    32701 gcaatacttt tctttttggg ttccctcaat ttgtttttac catactgctc cagttttgcg
    32761 gcagcttggg tacttgacaa accgttaaca gcatctgttt caagctgttt tattatttcc
    32821 tctttctttt caaaatgcgc tatcaaaggt aactcctttc cgtattttgt acttataaga
    32881 atattatact aaataagata gatacataaa atatatactt ccatacctac atatctacat
    32941 atctacataa atataatttt taatatctat tttgtctgtc cgtaatatgc cgactgtcca
    33001 tgctttctct taaaatgctt atccagaaga agtttgttaa ttgactgaat gtctttattg
    33061 gctttttcag taatgaaagc cattttagca atttcttcta atacgacaga gtgaaaaaca
    33121 gcttccatag cgttatctcc ccaggtaaat ggaccatgat ttcttaccag acatgccggt
    33181 attgcattat agtcttttcc tgtaaaggtc tcaacaatca cctttccggt atttaactca
    33241 taatcatttt ttatttcatc agacaataaa gcccttgtac agggaatttc accattaaaa
    33301 tagtcagcat gtgtagtacc cataggaatt ataccttttg ctgcttgcga gaatgaggtt
    33361 gcgtatgtgc tatgcgtgtg tgcaacggca tgtgtattaa taaatgcttt atatatctct
    33421 atatgtgact tagtatcact ggatggtcta tattttcctt ctactacatt tccatccata
    33481 tccaccacaa ccatatcctc agacatcatt tcatcataat ctacaccgct tggctttata
    33541 actatatact ttctttcttc atcaatggca cttacattgc cccaggtgta taagacaaga
    33601 ccttttttta ctaattcgat atttgcttta taaacttctt tttttaacaa ttcaaacatt
    33661 aaaaacctcc atattctgag agctatattc taccattatt ctgatatttt acatacccat
    33721 acttatattt atatttaaaa atcactaatt aattgatata cagctctcct gttttcatca
    33781 cttactgtgt tttcattaaa gaaaacaaca tccttcagat ttgaatgatc ttctttatac
    33841 tctctaatcc agtttccgat attctgaatt atttgtggtg tttttatcca tatatcataa
    33901 tttgaaagtg atttgttcct aacgcataca tgtagaataa tatcatttgg atatatactg
    33961 tccaaaagat tgttaaactc cgtctgactt attggaaaat gtgcaatgtg aggcataata
    34021 caatctacac cggctccttt caattttctt acttcatgac cccatccttt tgctaatgat
    34081 gtatgtaaac atatttcaat atcttgatta attgacctta tacagtcatt catagttttc
    34141 agtttttcgg ttatcttttc tattttccac ttttcaaaaa tttcatcatc tcttgaataa
    34201 tctgacagct ttaaacctgt atcttgattg aataataatt ttgtgtaatc acagaagcat
    34261 gaacctccag cataactcaa acgttcgtag cttatacttc ttacattttc atgattatat
    34321 aattttagaa gatcgcttat ctcatccaat tccagttgcc atgctccagg actgtcgggg
    34381 cagaataacc acttgtaaat atctccgtct ttattaatct gttcattagc tttcggatat
    34441 gattttatta tcctttttgg gttatatatt cccagattgg taaacagttg aatacctatt
    34501 cctctttttt cagcctcttc tattattttt gaaacaaatt ccgtatccat attagcatta
    34561 gtacaggttt tgtcacataa atgcttcagt ctcttatctc taactggcca gcacaatcca
    34621 tcatgcattg gatcaaaata tgaataactt accatcatca aactaagcat attcatgtca
    34681 tgctccgcca tttcatctaa aagcataagc attttgctca tgcttactcc ttttttttct
    34741 gttgattcat atgtaatacc agtctgccac cctatgtgaa aatttttatc caatgaagta
    34801 atcttctttc atgataatta tgtgctgaac aacagaaatt atatcataaa cagtaagagc
    34861 agatgtaaaa tctgctctta tcagtgtaac cgctttttgt attattcttt ttctttaatc
    34921 tgattttcct ttatatgcac atccatttga ggatatggta tctcaattcc ttgcttgtca
    34981 aattcctttt taacctgctc tgtaatgtca aaatatactg tccagtaatt tggcgcatca
    35041 acccaggccc ttaatatgta ttccagtgaa ctatttccat gtgtaaataa tttagccata
    35101 accggaggat cctggtccac cataggatga ttttttgcaa tttctaaaag tatactgttt
    35161 accttatcga catcatttgt atatgatgca gtaaatttta aatctacacg tcttttatct
    35221 ttcgcactga agttagtcac tgatgcactg gtcattgtac tgttaggaat tgttataagc
    35281 ttattatcaa tggtaagcag tttagtataa aatatcgtaa ttgaatcaac agttcccata
    35341 tgagtaccat catcaataaa atctcctact ctgaatggac ggaaaattag tatcattatt
    35401 cctcccgcaa aattagataa agcaccttga agagctaaac ctatagccaa tcctgcagaa
    35461 gcaagtactg ctattattga tgtcagggga actccaagta ttcctattac agatataatt
    35521 agcagtaatt tcatagttat agacaatgca cttttcgtaa aagtctgaac agttttatct
    35581 atcttaataa agccttttga ctttactatt gcttttacta tgtatttaat tagtagcagt
    35641 ccaacaatta gtacagctat tgccagtaaa atccttaatc ccactgttgc tgacagttct
    35701 attaatgtgt cccataattt ttgtagtgtg tcactcatac cttctcttcc ttctatttat
    35761 ttatattaat attatatatc aataacatca aaaaaacttg aactattcct atttaattct
    35821 ctgttgtctc ttctaaaact acaaaagtgt ttttccctgc tgcttttgcc ttatacaatg
    35881 ccatatctgc acgattaacc gctatactga actgatcatc actgctatag aaatctgata
    35941 ctccaataga cactgttatg ttgaattttt ttgcttcttc acactcaaat tgcatttctt
    36001 caacagtaac taaaagcttt ttcgtaaaat tgcttacatc ttcttttttt agtccattgc
    36061 atataagcaa gaattcttct cctccccatc ttgataaaaa gtcattactt cttatatgac
    36121 tatttatcgt atctgctaat ttaataagca cctgatctcc aacactatgg ccacaactgt
    36181 catttacttt tttgaaatta tcaatatcaa tcatcattat ttttgcgtta agtcctacgc
    36241 ttttaaaatt ggcccatgat gttgcaagat atttatatgc agctcttctg ttatatgtgt
    36301 tggtaagagg atctttcaaa gattcttcaa taagtgtctt gttaaagttt ccataatacc
    36361 atctttctaa atgatgaacg acaaccagag caattataat tattacaata actatactgg
    36421 atagtattat tagaagcatt ctgacctgct ggcttttatc ttgtgttgtt ttatcaacat
    36481 attcctgaat atcatcaaga tgaacaccca ttgcaataat ccaatcaaaa tctttataaa
    36541 gcttagcgta tgttatcttt tctgatattg tatcgctatt taatttcttg aaatagtatt
    36601 gaaagaataa ctctccgtac tgtttcactc cttcaagttc tgtcttgtat ggagtcgttc
    36661 ccatgacatc tgtcatattc gtagacaaat atataccttc tgtatctttt aaatttggat
    36721 gtatccgtct tatagcataa tcatccccac cagcataatt aactatttca ttaacccaga
    36781 catatgcatc atcttcaaat tttagcccat gtatctcatt atataatgca ttttttactc
    36841 ttgagtctat tacatcctga cttactccta ttactgtttt gtaattgcca tataatttac
    36901 tagtatatac aggaagttct gaggataaat ggtttatgta ttcagtactt ataatgttct
    36961 catcttccaa tgatggaatg tattgaaaga caacttcgtt attctggcta ttgataatca
    37021 gaaaagtgaa atctatcttc tttgcttcct gctgcatgta ctgctttgca taataaagaa
    37081 atccctcaga gtctgctaaa tagtaattat caaatattcc aatcatatcc tctgcaagat
    37141 gctggtgata aattacctgg ttttcatttt tctgctctat cccatctatt atgttgttta
    37201 cagtgtcttt aagatatcct tttttaacat ccattatgct gtcttttgca tattcatggt
    37261 atgcttcttc taaatcaatt aatacgaaat aaactactcc gcttataatc agtccaatta
    37321 caagtattat tgaaataagc agaatcctat atttttttct taaataatcc atataaactc
    37381 ctgtaacatt actaactaca tattatgtca actatcatgc atctcgttat gagtatatat
    37441 tattttttaa tacatattca atggagtttg aaatatatta attccaattg agttgattta
    37501 cgttaatttc tagatatttc catccataca ttttatatat tctgctacta aaaaagggtt
    37561 ctgaatatcc ttttcatcca aacctctgaa gcagtataat tcatcccaca atgtttcgct
    37621 tccttctata tgaaaaaatc ctgttacctg atctattcca ataaatatcc tcatatcttt
    37681 gtatatatct ggtctgttaa tgtaaaggct tttgctctta tctgtattta taatatagaa
    37741 agtcttaata tctggtgttg tacctgaagg cagcttttct aaatgacatt tattattcat
    37801 aatattggat ttgactactt ctgataattt ttcatcattt attacttcta agcaatactt
    37861 cttcataaga tattctatta tctcatttcc agacttccta ttcggtttaa gtttatcctt
    37921 atactcattc caaattcttt tccactgttt tatgtccttt gatgtagctt ttcttgttaa
    37981 cattcaaatc ctccaatatc cctttatgac catagtattg ttacaaccat cacctctcta
    38041 tattatatct ttttaaatct tatacgtaca aatttgcttt ttcctctaca tatcactgaa
    38101 tcgttacata tagcataatt tatatcatta ataatatcac catcaatctt aatagcatta
    38161 ccctctatca atcgtcttgc ttctgaattt gatttagcat atcctgttaa tcttaataaa
    38221 tcaattatct gtatcttatc ttcatcaatt tccattactt tcatctcgtg tggtatcttt
    38281 ttgcatgcga tactaatgta tctgtcctct gctggtttaa taaaatcctt tccatggtac
    38341 atttctatta tctttcttgc atataccatg tgcccttccc ttatatcctc attaacaacg
    38401 gcactaaact gctcttcggg aatatccgtt gtaagtctga agtagtcatt aagtaagtta
    38461 tcaggtatct tcatacactt ttcgaatatt gtttctgccg actcatttat accgatatag
    38521 ttatccaaag acttgctcat cttttctatt ccatctaaac cagcaataag cggaaaggta
    38581 attacttcct gaggctcctg accataatcc ttttggagct ctcttcccac taaaagatta
    38641 aatgtctggt ctgtacctcc tatctctata tccgcattaa ttgcaaccga atcatatccc
    38701 tgaattaaag ggtatagaag ctcatgaatc cctattggct ggttatttga gtatctggat
    38761 gaaaaatcgt ccctttccat tattcttgca agtgtatatt ttcctgcaag attcagaaca
    38821 tcagcaaaat ccattttccc tagccattct gagttatata ctatctgcgt ctttttcgga
    38881 tctaatacct ttgcaacctg ctcaaaatat gtctgtccgt tttttctggt ctcttcaaag
    38941 gataaagaat tccttgtctt gctctttcct gacggatctc ctatcatacc ggtaaaatca
    39001 ccgataacaa agattatctg atgtcccgct tcctgtaata cctttaacat cctcaaaggc
    39061 accgtgtgtc ctaaatgcag atcaggcctt gaggggtccg ccccaaattt aacctttaat
    39121 ggttttccac tttccagttt ctttctcaac ccttcttcac taaacacatt cactgcattt
    39181 ctcattaaga tttccattac tttttcattt ttcataaatt ttctcctcat aataaaagac
    39241 tcaagcgtct gcttgagcct tatcattcat aatttaaaac acaaatcatg acaatagcaa
    39301 cccaagccta cttttgtagt aatagttata ttgatatgta taagtgttat tttgtatact
    39361 tgctgccatt tcaaatacct ttcataaatt aaacgcagta tatcaataca gtctcaaaat
    39421 gtcaataatc tgcatctggt atgttctaaa tactatacac tctaaatatc tttttttaaa
    39481 aatctacttt attcaactca taatcaatag atgtctggta ttcacctaat tgatctttcc
    39541 atgcattttc attcacacgc agcttcttaa atccaagctt ttcatataca tgctgagctc
    39601 ttgtgttttt tacattggta tcaagtatta tctttttata acccatatca gtgaataatg
    39661 aatttataag catagatagc aactttgtgc catatccttt ttcctgcttt gaaaaatcac
    39721 atatctttat gcctatttca gcagtttcat tacccttatt cctgtagttc atttcaccta
    39781 taggaatacc agcatgttca attataagcc ttctatgtat ttcatcatta tcattggaaa
    39841 gctgtttgat tatttcctcg tcagttgtac aaagaccatt cggaaaacca gcatgagcca
    39901 tgactttccc gtcgttccac catttgcata ggatatgagc atctaaagct gttgcgtccc
    39961 ttattactaa attattgtgt tttattacca ttaagctact ccgtctggtt atctattata
    40021 ttttttcttg acttgtcaat acatctttaa caatattccc atgaatttgc catacattat
    40081 caccataatt gctgattaat gttatgagta atttcttgtc tttattgtaa cttgagataa
    40141 agctcactcc tggatctgat ccttggaaat atgggatata cttagattct attttttgta
    40201 gccatatccc atacccataa cattcctctt cattactatg atttttgagc atattttctg
    40261 tcatatccaa agataacaac ctgtaattta acagattatc ccagaataat ttaatatcat
    40321 ttacagttgt aaatgctcct cccgctccgg tgccttttgc atcgacacaa tagatattag
    40381 tgcgatattc acttcttatc tcatcataga tatagttaga agcgcattta gcaggtaatc
    40441 tgtctaattc ataataacag gtactatgca ttttacatgg aataaagatg ctctctttta
    40501 aatacatatc gaaatcctta cctgttatct tctcaattat catagctaat actacaaatc
    40561 cggtattgtt gtatttaaag cggcttccat gtggatacat cataggctta tcaatgaaaa
    40621 gaggaagcag atcagtattt gaccggattc tataattcgg aaaatccttc caaagctctt
    40681 catattcatc cattaccgat tcgtcaaaat agtccggaat ccctgaagta tgagttaaaa
    40741 gctcttccac agttatattc ccatctatct gccttaaatc aaaatcaagg atttcagaaa
    40801 tcttcgtatc aaatcttaat atcttttttt ctatcagcat cagtataccg acagcaacaa
    40861 ataccttacc ggctgaagct gtagcaaata ttgtatctat atcattagaa acttcattag
    40921 ctaaatccgc atatccatat gctctttcaa atattctcac tccgtctttg catacagata
    40981 cgcaacctct gaatttttca tctattagtt tttcgattcc catgcctttt ctcctataca
    41041 actctatata attacattaa tgaatattcg ctaatacatg cacattgatc cattttgcta
    41101 ctccgtcatc atcgtttgtt tccgtaatgt aatcagcagc ttctttaaca attggtaaag
    41161 cattttctac tgcgacaccg ataccgcaag ctcttaacat atcgatatct ccgtaatcat
    41221 caccaaatga tacagtatca gatagttcta ttgaataata atccagtgct ttttttattc
    41281 ctttaagttt tgtagcgtct ttgtgcatca ttagcccaag cttcttatct gacatttcaa
    41341 tgtaaacacc atcaggaaga atatctttta ttttttcaat ttgctccata gatgaaacac
    41401 cgactattat cttatacgaa gcaacatcag gaagatcggt aaaatcagtt tgtttggctg
    41461 aagtattctc ccaatgtact gatacatcaa agttagcata tagtacatcg tctatctcca
    41521 cagataaagt cgattcggga tagatttttg caatattaga aagtatattt tttgtatcat
    41581 ctatagatat gcgttcttta tacttatttt ccctttcaat atatacagca gccccattat
    41641 gccagataat ggcatctgga tttaaaagtt tgtcatattc ccagattgca cgtatagggc
    41701 gggctgaggc aataaatatt tttatgcctt gttcatgaca cctttctaac acagatagag
    41761 tgaaatcaga tattgtctta tctgacttaa gtagtgttcg atcaaggtca agaactatag
    41821 ctttaatatt catcatttat ctctttcttt ttgttattta aaagcataac ccagtgcttg
    41881 cttgaataat cagtattatc tttgtattca gctatttcaa caggcttgag ttcaaactcc
    41941 cagtcagtaa aaaacttttg
//
LOCUS       DAOSXM010000012        41211 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2111
            NODE_608_length_41211_cov_10.155287, whole genome shotgun sequence.
ACCESSION   DAOSXM010000012 DAOSXM010000000
VERSION     DAOSXM010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864178
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 41211)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 41211)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:24:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,261
            CDSs (total)                      :: 2,229
            Genes (coding)                    :: 2,218
            CDSs (with protein)               :: 2,218
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 3 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41211
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_608_length_41211_cov_10.155287"
                     /isolate="AMR_MDS_2111"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            75..1364
                     /locus_tag="PLQ68_03135"
     CDS             75..1364
                     /locus_tag="PLQ68_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXK71677.1"
                     /translation="MKLNYKRTFLVGLAFLSISMFWQMYDFVIPLILLNKFGIRVDIA
                     NSIMALDNVLGLFMLPLFGVLSDKVSTKIGRRMPFILVGTAVAAIFTVVLPIAANSNN
                     LLLFMISLGLVLLAMSTYRSPAVALMPDVTAKPVRSQGNAVINLMGTLGGTIVLALKM
                     FIKPGPDWDYTTFFITVATLMVISVLILFITVRENKYRKEMEDINYGDPIIEQRSDDI
                     TEPKAEKLPKDVMKSMIFLLGSIFLWVFGYNAVTTAFSQYIVNYFALPEGLAAGFLMV
                     ATISGVITFIPIAYLTARIGRKKTILGALTLGMIFFAIAAMITDFSFYTNFLFVGVGI
                     VAGTVTVNTLPMVLEMSKGATIGKFTGYYYTCTMSAQILTPIIIGRLFFKIGYQVLFP
                     YAVAFLVLSFIAMLFVKHGDSKPQVKKKKLETFDLED"
     gene            1367..2203
                     /locus_tag="PLQ68_03140"
     CDS             1367..2203
                     /locus_tag="PLQ68_03140"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HXK71678.1"
                     /translation="MEWLIILVMLIIIWLLMIWPSDYIKDDKKKLRKYLYAHRGLHNN
                     EKSIPENSLAAFDAACEAGYGMELDVQFSKDMQIVVFHDDDLKRMCGVDKKVFELTYE
                     ELSELSLAGTEHKIPLFTDVLKLVDGRTPIVVELKNCKIYEKLVIETNKILRNYKGDY
                     CVESFNPLIVRKYAKTNPMVLRGLLMTNHVKAKSINVVLGFMIQEMLLNFMARPQFIA
                     CDHKSLNIWGVNVVKFIFRPVMAAWTVRNEDEFEKVKNFDIVIFEDFLPDPSRPELVH
                     NK"
     misc_binding    2379..2571
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            2598..2843
                     /locus_tag="PLQ68_03145"
     CDS             2598..2843
                     /locus_tag="PLQ68_03145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK71679.1"
                     /translation="MDTFLIVISIILCVIGALVSFMSRFIVRKMNIASSQVIKGIEDE
                     KAIESLKEQKAIMKVKLVGAAIFIPGMIVLYIILKGM"
     gene            2901..5546
                     /locus_tag="PLQ68_03150"
     CDS             2901..5546
                     /locus_tag="PLQ68_03150"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004616301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HXK71680.1"
                     /translation="MKILETKYDPSAIEDRLYSFWEQKGYFKANPDNSKKPFTVMMPP
                     PNITGQLHMGHAIDNTMQDILIRMKRMQGFNALWLPGTDHASIATEAKIVESMRKEGI
                     DKHDIGREEFLKRAWSWKEKYGSRIVEQLKKIGSSCDWSRERFTMDEGLSKAVVKAFI
                     NLYEKGLIYRGEKIINWCPVCGTSISNAEVEYEEQDGFLWHIKYPIDNENGFVIVATT
                     RPETMIGDTAVAVNPNDERYKHLVGKSVVLPLVNKRIPVIADEYVEMDFGTGVVKITP
                     AHDPNDFEVGLRHNLPIINAMNDDATMNENANQYAGLDRYEARKVMVKDLEKQGLIDK
                     IEKHTHNVGSCYRCDSVVEPRVSTQWFVKMEPLAKPAVEVVNNNETEFVPSRFSKIYL
                     NWMEDCHDWCISRQLWWGHRIPAYYCDDCNEIMVAENRPEKCTKCNSTNIHQDEDTLD
                     TWFSSALWPFSTLGWPDKTEDLKYFYPTNVLVTGYDIIFFWVARMIFSGVEYTGVTPF
                     SKVLIHGLVRDELGRKMSKSLGNGVDPLDIISQYGTDALRFSLITGNSPGNDLRFSDT
                     KVEAGRNFANKIWNATRFVLMNFDEYVDFSDINRDNFENPEKWILTRLNQVIREVTDN
                     MEKFELGIALQKIYEFIWDEFCDWYIELVKPKLYDNQNSKRKEALYVLNYILTNAMKL
                     LHPFMPFISEEIYQSLADSEESIMISSWPSVEMMNEYPEYAEDMDVIMNSIKSIRNKR
                     AELNIDVKMKSNIFFVSEDEHTQNLLQQGEQYFIKLASAKAVSVFKNEKDIPDNSISM
                     ILPGFKIFMPFKELVDIKEEILRLEKEYEKATSEMERAKSKLENEAFVSKAPSNLVEA
                     EKQKLKKFEDLAQDIKNSLENLKCM"
     gene            5537..6493
                     /locus_tag="PLQ68_03155"
     CDS             5537..6493
                     /locus_tag="PLQ68_03155"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HXK71681.1"
                     /translation="MHVIDAHCDTLLAMCEKKENLQYSYELVKTLRFHYMQFFAMFCP
                     PSLLKEEDGFAKSEKLLHMMMNLYQKMTEQYSYNKVLCKNDIVDFEKKKDDKNPSFFS
                     LLSAEGVYLSKNDISYIDYLYKNNVRCLSFTWNPSNEFGQGVNGDSKKGLSNLGIRAV
                     SRCNELGILVDVSHANDRTFHDIASTSKKPFVATHSNSRYVCAHKRNMDDDMLRTLAK
                     AQGVTGINYFSDFLVEKEKNKTADFFDIIKHIEYIAALIGVNYIGLGSDFDGIDKAAI
                     DDVTKVPDIINMLLKLNYKEDEVNNICSGNFIRVLKEVLHDE"
     gene            6483..7517
                     /gene="mtnA"
                     /locus_tag="PLQ68_03160"
     CDS             6483..7517
                     /gene="mtnA"
                     /locus_tag="PLQ68_03160"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010293469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HXK71682.1"
                     /translation="MMNEITPIEFRDNKLILIDQRKLFDSEEFFVCDSIESVYFSIKE
                     MVVRGAPAIGVTAGYGMYIKALSIKTADLDEFRIEMKKAAEYLDSSRPTAVNLSYATK
                     RIMSVVNENWDEVGELKNALYKEAIAIHQEDIDANRKIGENMLSLLKNAKRVLTVCNA
                     GILATTSYGTATSAMYLAKEQGKELIVYACETRPRLQGARLTAYELNKNGIDVTVITD
                     GMVAHLMSKNMVDAVIVGCDRVASNGDTANKIGTKNISIIAKRYNIPMYIACPTSTID
                     MDIEDGSKIVIEQRDSVEVTHINGVRIVPPGVKVFNPSFDVTDNADITAIVTEKGIAN
                     PPYNVTLKIL"
     gene            7738..9576
                     /locus_tag="PLQ68_03165"
     CDS             7738..9576
                     /locus_tag="PLQ68_03165"
                     /inference="COORDINATES: protein motif:HMM:NF019552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HXK71683.1"
                     /translation="MTGYNWEKIIKDFDANSSIYDSIKDNTDEFIDSYKDHVEWKAGW
                     GHDFHCDYCSGRLIFDKTNRGKYTCKVCGKENTGDRKDWSWTTSYRSASCTHVKHAGI
                     LYSITKQQKYIDYIRKVMNFLTDNYDKFESFAPNLRFLGRYTGTGLSDAHNTMSLLYG
                     IDAAQDHLTKAELDKWHKGFFTPQADMFDFFSNKIYNIPLWQKVSEANIGVFFKDETI
                     IKRAFYSKFGILDQLRRGVTEDGMWFETSTGYHFFGLAATAELLLLCRRNEVLCIPEH
                     QELMDIIESQFMFPLKIMFKNGCIANPCDSGLDMDIFTYQNAYVKACALFDNPYFKQV
                     LGYIKHIKNQPRTLDEILYSIEYDECDHLPDFGSNNFEDSFCAILKNDVTELYLKTGI
                     KTICHAHPDVMTVEMAFYGDLLSKDIGSSGYSSKIFNEWQHKTIAHNTITINRQDQTY
                     TPVGEGIWPEGIVEYYDEQHIRAKSKNVYECVDYTRDMKVKENKIYDQFSVKAVEDLE
                     LDYAFYSKGECIIENEYEKVDSIGESEGYQHLFDIKKIDNRDTVTVKFELEDKILSVT
                     IEPDEDSVVYIVNSYVNNFNDTRYGIIIRRNGKERIFNVTYECVMK"
     gene            9584..10276
                     /locus_tag="PLQ68_03170"
     CDS             9584..10276
                     /locus_tag="PLQ68_03170"
                     /inference="COORDINATES: protein motif:HMM:NF013782.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HXK71684.1"
                     /translation="MHTYETHIIKESMCDCFRRLKLQHMFIMMQDAAYKDLLTYGCGR
                     KSLLDKDVAFLLRRSVVEVINAPRADDTVVVGTSQISLQGMTYTRDFIFICNDEICVK
                     ASTQWFLCKISDKSLIRPTIQTISYSGDSVGISIDNKPEIPDDRGEIYNTIKIGYSAI
                     DGNMHVNNTEYVSWVCDAPNAISYLEEGHYSFDIYFKKEVIPHDTVDLYQINNVVYGI
                     SQNQTSFIAKFK"
     gene            10526..10885
                     /locus_tag="PLQ68_03175"
     CDS             10526..10885
                     /locus_tag="PLQ68_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004605308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2089 domain-containing protein"
                     /protein_id="HXK71685.1"
                     /translation="MKNFVPSQCPICEGELVITDMECKNCRTHISGEFYTTKLAKLDP
                     NQLEFIEAFIKCRGSIKEMEKELKISYPTVKSRLEIIAKTMGYKDEKEEDDTRMKVLD
                     SIEKGEMTVEEALKILK"
     gene            10898..12094
                     /locus_tag="PLQ68_03180"
     CDS             10898..12094
                     /locus_tag="PLQ68_03180"
                     /inference="COORDINATES: protein motif:HMM:NF024743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HXK71686.1"
                     /translation="MNESKEIEMILKLLEEKKITTEEAKMLIEALKTANNNQSEDQDT
                     YENDDKSGSENVFCDDFDDVRTKFSNFGFDIKDTILDKMSEIKSNVFDSLSFDIPSNT
                     DLFDLKSDKMDFELNDKASISIEGTNSSVIVKHGSGDKVLINFSVKSRDYDRIHEIIE
                     IQYSKDFLGITVDDAYKERIVITIELPDIDLLKLKIETTNGPISLSDVKCGQIHLEGT
                     NGSISLRRCTGNAFKAETSNGSVRCEQNSFDESIIVESSNGAVYSNMDNCKLIDMETT
                     NGSIKFYGSCARSFKAETTNGSVIFDRLNPYQSESKFLLMGETTNGSVTAYINRNYGV
                     VFECSSGRHGNIRLGRNFNMQSMQRNSIGRISYAKGESRYAYKSDIVAAVETKTSNGT
                     IIMEFV"
     gene            12103..12249
                     /locus_tag="PLQ68_03185"
     CDS             12103..12249
                     /locus_tag="PLQ68_03185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK71687.1"
                     /translation="MKRGTMVRFYYPDYISEDNAEVNVSQKINYRPSSSVWTNNQFYS
                     KYLK"
     gene            12404..14155
                     /locus_tag="PLQ68_03190"
     CDS             12404..14155
                     /locus_tag="PLQ68_03190"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXK71688.1"
                     /translation="MDKKSSKKQKTKASDVIKMMVRIYKETSEHKKWGIARWVGGLLS
                     GAVAVYEYYFIGSALDVALSNDYKRLLSFIYALVGIFVFRIILQFTNPLIDGQYELRS
                     YRTLSLNAYRKIDKLQMGYYENVHTADTISTLIDDIEKIKTFMGNSIAGLLSWNPITI
                     ILSVIILCTINWKLTIFSLIIVPVVMLVLNSVSKPLKDTKNNIQEYTAVFNSHLRDFI
                     EGNDIYKAFNMHRKHTEKFEESCQKIANESYKSSRVMAKNRALNILMMVIPQGICMFA
                     SIFFIARNELTIGQYVIFSNMLWPLLSAFRTTGIAWSEMVGYSGTAQRYFKFLEYNEE
                     RTGGEDFGVNDSEIIVEFNNVSFSYHDNIPLLRNVTFKLYRNSRLALCGISGSGKSTI
                     FKLICGYYENYSGDIIVFGHNIRDWNLNALRKYMTCVTQDVFLFDDSIVNNIRMGKLD
                     ATDEMIEEASKKAYIDQFINDVGERGGKVSGGQKQRIAVARALLKDAPLIMLDEPTSA
                     LDTKAEYYVQQSIENLKQGRSVLVIAHRLTTIIDSDIIIVLEEGKIIEQGSHDQLIDR
                     NGRYSELYTRQLIEQGV"
     gene            14157..15959
                     /locus_tag="PLQ68_03195"
     CDS             14157..15959
                     /locus_tag="PLQ68_03195"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXK71689.1"
                     /translation="MIKDMKFILMLIGKNKAKYFTLLPLWCIIGSLVDISLSYALKLL
                     TDYFMYKNSQNITNLIIIFSATIITSILFCIIFHLHNKVKEDILRDIRIMTFKEVQKL
                     PSSYFKSTHSGDLVSKFNADTQSVTTVFGEFENIFESIIAMFIRIPFVLLLDVRFGAM
                     LLGFSAVTGILNALFIKPLREKHKVILENKAVLSKEASEAVTGFTAVKMLSLSSHLLK
                     KFTNSVQDNFNSEWKAKKTYTLQRGLNNIIWNSSYLLTTILGCIFVIKGTLEPGNYLA
                     ISTGNGIGWSISVLISALPNLQKAFAGSNRLKTLYDAQKEPDRYQIDGTESDMGLDVQ
                     DLEFSYEMEKENNKNNENNENNNTLDNEESDYTSYQFKISGLSFSINKGDTLALVGDS
                     GGGKSTIAKLLLGLYMINSGSIQVNGQAYKDYSLEELRDQMGYVPQDAYIFNGTIREN
                     ILYGNTDADEQQIINAAILANAHDFILAQPDGYETYVGERGIRLSGGQRQRIAIARAI
                     IKNPSILLLDEATSSLDSESEKLIQDALDRFMHNKTSVVIAHRLSTILNADKICYIKD
                     GNVVEEGTHNELLSKKGYYHSLYYREFANKTNES"
     gene            complement(15946..16947)
                     /locus_tag="PLQ68_03200"
     CDS             complement(15946..16947)
                     /locus_tag="PLQ68_03200"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HXK71690.1"
                     /translation="MKKRICFRTKITHYIILILVLSFLTITAASDYDRAYVQKEISNL
                     SLQALSPYKNTQGIEYWPLCTSKNNQPRFVTGTNPHQGTDLTINVGEHIYPIYDGEII
                     YIKKDISSQTGHIVVKSEIGYTEPVYIEYLHVIPIDGIDEGDFVYTCIPIATIDEYKR
                     YDSHLHIGRVNEDRTLHYQIYDLYKDVLNWKNGSDLDVFSYPDFNTETNIFTITAYVS
                     SDTENTDYYAGYGRFPLEYITFFYSVNNGTWKSKNIHEYSDNFKYSFNVKQITGSKSN
                     DTIRYYITATRDNNSSLDTTFKDASYKTAYYPAYYAHPGPALTNQLADAIALTITIH"
     gene            complement(16919..18070)
                     /locus_tag="PLQ68_03205"
     CDS             complement(16919..18070)
                     /locus_tag="PLQ68_03205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK71691.1"
                     /translation="MIKEFLRKSRQNIVLMLVILTMAILFASCNGAEPTQSITDTPSN
                     QLTQSPIQPSEEPIEEEPDFMEFDMIMQYQNGIQSMPLTYDYNNLTLTATDGYTVSIP
                     VVDFENRLYPIYQSRSNDTLNTSLIAIPNSSDSLYILWNQHKIYYVNLSKQIMDTLLL
                     EEDDDTTYDKSLEENEANGLYWGTKPKISENGKYLIYLTNRRNNGKTNDIRLYDFETK
                     EDILLVEDSFYNDAYISETTIFYTCNDMLWRVDIASKIKSPVSYSISSNGNFSYPYYI
                     YTKTYYQEYEILNLLTLNVEKGKLGSQSSALKISAMRTDTLNVASVVLLDNKNVTVTF
                     LDLQSNEILKTFVVSESFLVVYAHWKNSKTFILSGYEGGSNEKTYMLSY"
     gene            18178..19392
                     /locus_tag="PLQ68_03210"
     CDS             18178..19392
                     /locus_tag="PLQ68_03210"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HXK71692.1"
                     /translation="MKRRNLSVILLIALFIMMLPVTASDDSYNRDQVSSIYSTTTVDN
                     LKATSAILVEVNTGQILYSKDEDKQIPIASLTKIMTVYLVMEAIAKNEISYDTMIKAS
                     ASAHGVEGSSVYLNLGEEFTLREMLYAIEVRSANDAAVAVAEAISGTQGEFVNRMNNK
                     ATELGMFNTYFKDCTGLTDAGHYSTVKDISIIATRLVLEYPDIFEFSRTLEKDFRDKE
                     HRDYQYMVNRNNLLNYYDGATGLKTGFTTAAGYCLVGSAQKADISLVAIVTGEPDNNH
                     RVAETAFLLDYGFSNFMYTKTDDDQKMVGTIEVRKGIDKTVETYADGTLTLFMKKSDI
                     SRISKEIVYDMATLEAPVEKDTVIGKVVYSLDEDILGEVSVKTSQSMEKAGFWSLLWR
                     SVLSWFGIDWQK"
     gene            19458..20132
                     /locus_tag="PLQ68_03215"
     CDS             19458..20132
                     /locus_tag="PLQ68_03215"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HXK71693.1"
                     /translation="MLQSIIDSMIPMLYMIPVMLISLTLHECAHGLMALKLGDTTARD
                     AGRLTLNPLKHLDPVGAVMMVIARVGWAKPVPVNPMNFKDPKNGMALVGLAGPLTNLI
                     VAFGAAFPVALIENAVYLHGVKLTTFLEIAYVFCYLLFQANIFLAIFNLIPIPPFDGS
                     RIMFGILPDRYYFSVMRYERIVGMAFIFIFFIFRSQFAVVLDFISSPITKSFLFVANW
                     FVGLFY"
     gene            20142..21131
                     /gene="trpS"
                     /locus_tag="PLQ68_03220"
     CDS             20142..21131
                     /gene="trpS"
                     /locus_tag="PLQ68_03220"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HXK71694.1"
                     /translation="MKNMVVLSGTRSTGSLHLGNYFGAVENWVNLQNKYDCYFFIADW
                     HSLTTGYEDTNNYVYNINEVVMDLYASGLDADKCNIFLQSKIQNHAELHLLLSMITPL
                     SWLERCPTYKDQLAQLSEKDIRTYGFLGYPCLMASDILIYRADAVPVGEDQLPHLEVT
                     REIARRFNHIYTEVFPEPKALLTKSKVLPGLDGRKMSKSYDNTILLKDKDDVLRTKIM
                     SMITDPARIKKTDPGHPDVCSVFAFHKVFNENEVPSICDACKKGEIGCVACKKNLYQK
                     VTEFMKPIHERRSEIESKPELIKKILEKGNENAKIKTDETMKLVRKAMKINWD"
     gene            21122..21889
                     /locus_tag="PLQ68_03225"
     CDS             21122..21889
                     /locus_tag="PLQ68_03225"
                     /inference="COORDINATES: protein motif:HMM:NF014654.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HXK71695.1"
                     /translation="MGLGYQIKLENFEGPLDLLLHLIDENKMSIYDIKISIITEQYLD
                     YLMAMQQLDMEIASDFLVIAATLLHIKSKSMLPKTKDEAVDSETDLIKQLIEYKKIKI
                     AGERFKEMHESGQKSFHAFASNEDFGESEKTYMLSKSLLAMMYRESMQRSVEKTNKKA
                     INIKEIVKKEKVSLAKSIRKVIDVLFKDKRISFFQRFKIHSLSKADLVTDFLSILVLS
                     SQRKAILKQKRPFEDIMVEGTENLSYPDNVHEIYDWE"
     gene            21895..22473
                     /gene="scpB"
                     /locus_tag="PLQ68_03230"
     CDS             21895..22473
                     /gene="scpB"
                     /locus_tag="PLQ68_03230"
                     /inference="COORDINATES: protein motif:HMM:TIGR00281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HXK71696.1"
                     /translation="MNKDYVINLINAMLYANGEPITLSRFLQILEMEKEDFFEIMNEH
                     IENLKKSKSGVLIRRVEDAYQMCTNPLYFTTVRQLFEKKKTETLSRAAYETLAIIAYN
                     KEATRAKIEAIRGNSDSPLKTLLERGLIIEAGRLEAPGRPVLYKPAEEFYRAFDVSAL
                     DELIPLDNFDEEPEEESSEILDNQKFTEQDSI"
     gene            complement(22466..23440)
                     /locus_tag="PLQ68_03235"
     CDS             complement(22466..23440)
                     /locus_tag="PLQ68_03235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK71697.1"
                     /translation="MFNKYINNPVIKRTPGTFYSKYAANPDILEFDNKLFFYFRGQDH
                     NNHDQMGVATSSFEAFNGVTWEYIEKEPILKVNSDAKAYDSNHILDPAAVVIDKKVYL
                     YYSAHSLEMPHSVSLAISEDGIHFEKYKNNPIIHNAVVPEVVIKDGMIYLFYQRWTAG
                     SKGVSKFYVCPSTNGIDFDISKERVVFEPDELSHSLSTNRIFYEDGMYYSFFGNNERF
                     KDYPESIGISRSTDLLNWEKSSDTIIRRGNRGSWDEGALWFATVYKHKDTYYLWYEGT
                     GTDNKRITQDDIEASDKAVSESYGGYGKTSFSQIGMATFQGKLTDYFK"
     gene            23600..24961
                     /locus_tag="PLQ68_03240"
     CDS             23600..24961
                     /locus_tag="PLQ68_03240"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HXK71698.1"
                     /translation="MELTNEQKKQIEQLQQDNNKDVISGYLAIITATVPLVIWPFFPQ
                     RFFFPKMVMLYAFALLMLVFLAALKLNIFSKRLFKEPAVYSVLFFIVYTTVTLFSSIN
                     MDLSLYGAHERKETYFVLVIYVLFFLVAANHYRYKKWHVIAFGIMIGLLCIYAIFQLY
                     GLELIDLTFLSKYKISASWHYIAFSTLGNPNFLASMLAMSLPIFVFAFIKSGNYFWTI
                     PMSLNYFVLLTTKTRGAWMAAFVSFTVLVVLMVIKKYSIKNIIICCVIHIGLTVLYAL
                     LFSGFLDRIVSAVDTVVTVVASDNEQKVMESGSFRIAIWKGVWMLIEKKPLTGYGIES
                     LKHAIFADDQVRMFLREISPQIVNTDKAHNEFLNICVSSGIPAGIAYLTTAIIVITKG
                     FMNMKKNPFLLPLTCSVTAYFIQGMFNISNNGIAFIFYILMGVIIALSKSNSNKIKLK
                     DNT"
     gene            24961..25677
                     /locus_tag="PLQ68_03245"
     CDS             24961..25677
                     /locus_tag="PLQ68_03245"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012058121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HXK71699.1"
                     /translation="MEKIRLQKYLANAGVASRRKAEELIAKGVVSVNGKIVNEVVLVD
                     DNDDIRVNNKPIKIQDKFLYYAFNKPEMVITSMSDQFGRKTVSDFFNKIEERVYPVGR
                     LDYDSSGLIFMTNDGDFANYLMHPKNQVTKTYSVYAKGSITSDIKKTLEEGVDIGDYV
                     TDKAEVFDIENNEGRCTAFISIKEGKNRQIRKMFEVVGIKVYMLQRVSIGQISLGDLK
                     KGRYRSLTAKELETLGYCKK"
     gene            25842..27380
                     /locus_tag="PLQ68_03250"
     CDS             25842..27380
                     /locus_tag="PLQ68_03250"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004617955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HXK71700.1"
                     /translation="MSMIEKVNELKNKKQEASLGGGQLRIEAQKAKGKLTAIERIHKL
                     FDEGSFVETDSFTQSRSVDFDMASKRKAGDGVICGYGRINGRPVFVASQDYTVLHGSV
                     GEMHAKKITKTIDMAIKTGVPMVYLFDSDGARIEEGTDVLASIGEFMAKQTMASGVIP
                     QISAILGNCAGISSLAPTLSDFVLMTEKTSYMYMNGPAVIESSTGVNPGKDDIGSALT
                     HTSLSGNAHIYYENEDKCIEGIKDILSYLPDNNLSEAVKVECNDDPGRASDDIYEVIS
                     DSYDMKDVITKVTDNSSFMEIQPEFGQGIVIGFARMNGDSVGIVANNPAVNQGALDMD
                     SADKAARFIRLLDSFNIPILSFTHTSGYIADAQQEKKGLIRHVAKMLYAFAEASVPKI
                     NVVTGKAIGGAFVAMNSKSIGADVVYAYPTTEISIMESEAAANVIFSKQIAQSEDPTT
                     FRKEKVDEYRQRYGLAYEAANRGYVDDVIDPAYTRTYVISALEMLKSKREDRPNKKHG
                     NMPL"
     gene            27399..27803
                     /locus_tag="PLQ68_03255"
     CDS             27399..27803
                     /locus_tag="PLQ68_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007866376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HXK71701.1"
                     /translation="MKKFIAKVNGREYVVELEEVGSTETKTVETVQTVQPVQQTVTQQ
                     EPKKQEEPAPKKAVTSGGAGSVKVEAPMPGTILNVKVSVGQSVKKNDVLAVLEAMKME
                     NDIVAAQDGVIASVNVANGDSVESGQLLVSMD"
     gene            27816..>28312
                     /locus_tag="PLQ68_03260"
     CDS             27816..>28312
                     /locus_tag="PLQ68_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358786.1"
                     /note="Converts oxaloacetate to phosphoenolpyruvate using
                     ATP as an energy source; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxaloacetate decarboxylase subunit alpha"
                     /protein_id="HXK71702.1"
                     /translation="MSVKITETILRDAHQSLIATRMSIADMKDALVMLDKIGYHSLEC
                     WGGATFDSCVRFLNEDPWDRLRYIRKHVKNTKLQMLLRGQNLLGYKHYADDVVEYFVQ
                     KSIYNGIDIIRIFDALNDARNLETAMRACKKEGGHAQGTICYTTSPVHTFEQYIKDAK
                     NLVDAG"
     assembly_gap    28313..28412
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <28413..29075
                     /locus_tag="PLQ68_03265"
     CDS             <28413..29075
                     /locus_tag="PLQ68_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516190.1"
                     /note="Converts oxaloacetate to phosphoenolpyruvate using
                     ATP as an energy source; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxaloacetate decarboxylase subunit alpha"
                     /protein_id="HXK71703.1"
                     /translation="VASLKDTPYDTGLDVAKINDISKLFKVIREKYIKSGLFNPKVMG
                     VDINTLIYQVPGGMLSNLVNQMKEANKEDQLQDVLNEVPKVRADFGYPPLVTPTSQIV
                     GTQAVMNVISGERYKMVPKESRDLVAGKYGKTPVEISQEIIDKILKGEKPITHRPADD
                     IPPALEETKNEMKEYFEQDEDVLTYAMFPKVAKDFFEYRRTLKLKLDKDLTDMEDKVH
                     PV"
     gene            29196..30065
                     /locus_tag="PLQ68_03270"
     CDS             29196..30065
                     /locus_tag="PLQ68_03270"
                     /inference="COORDINATES: protein motif:HMM:NF013539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HXK71704.1"
                     /translation="MSIKDKLKNTKGFSKGQKKIAEYILKNFDKAPFYTATKLATEAD
                     TSESTVVRFAIELGYDGYPEMQRDMKEVARQTLTSAERLMRISDTVELNNVLMSVMKS
                     DIMSIKETINMIDNDVFNKIVEKIADAKRIYILGIRSSFSVALYMSIYFKMIFDDVYL
                     IGMPGAADIFEEIISISEDDVFIGITFPRYSKRTIHAMDIAKNSGAYTVAITDSKDAP
                     ISEVAHNILVARSGLTGFIDSLTAPFSVVNALIAACGIKKDKELMTRLQNMERIWDEH
                     SIYTIETKKINEK"
     gene            30055..31299
                     /locus_tag="PLQ68_03275"
     CDS             30055..31299
                     /locus_tag="PLQ68_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HXK71705.1"
                     /translation="MKSKYDLIVIGAGAGGMMAAITAAQRGFSVLVIEQKKQEGSKIL
                     VTGKGRCNITNDCDVQDILKNVMTNPSFLYSSLYAFPPSRIIEFFNGIGVQTVTERGR
                     RVFPESNKAIDVRNALVKQMQKAKVEVLKNTKAVDVIKNKDLFTVTIKDVNDVKDDYN
                     CTNLLLATGGMTYSSTGSDGSGYQLAKKLGHEITPLKGALSGLLTKEKWVKETMGLTL
                     KNVTLSVYKDEALIYKELGELLFTHFGISGPIVLTASRYLLDTDYKNTKAVIDLKPAL
                     DEETLYKRITSDFEKNSSKIFINSLDALLPKSLIPIIVRLSGIESDKIVNQISKDEKK
                     NLTHLLKNLTLTIKSGMDMEDAIVTKGGINVSQINPSTMESKITKHVFFAGEIIDVDA
                     LTGGYNLTIAFSTGYLAGQSIY"
     gene            31406..31825
                     /locus_tag="PLQ68_03280"
     CDS             31406..31825
                     /locus_tag="PLQ68_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014313615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HutP family protein"
                     /protein_id="HXK71706.1"
                     /translation="MDYDTIGSKDTSKAAIKLSITSTREEEYSLIRDYAEKGIKCVAV
                     DFGGDFIGSVSKIIERAVVASKRQNVIADVHHEQGAVVGATREALGQIMPKAIGCNVG
                     GKIGIARHKDHITVAVFTGIGFLNLDEIGIGLGHRSL"
     gene            31822..32487
                     /gene="cmk"
                     /locus_tag="PLQ68_03285"
     CDS             31822..32487
                     /gene="cmk"
                     /locus_tag="PLQ68_03285"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004624110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HXK71707.1"
                     /translation="MRFSAIAIDGPAGSGKSLMAKMLSEKKGYTYLDTGAMYRTVALK
                     AIRNGIDTLDGDKLSKLIKNIDIELVITDNKQHMYLDKECVDDFIRTNQISKGASDVS
                     KFPSVRMFLVDMQRNVAKKHNVVMEGRDIGTYVLPDAEFKFYLTASNEKRAERRYQQM
                     VQDGKETTYEQVLEDIIYRDNQDSKRDFAPLKKADDAILIDTTNMGINEVFDTLLRYI
                     NGK"
     gene            32477..33076
                     /locus_tag="PLQ68_03290"
     CDS             32477..33076
                     /locus_tag="PLQ68_03290"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HXK71708.1"
                     /translation="MENECFRKTCRAIVRGIIKIFYRVEYIGEAYPDEGAYIICPNHF
                     SILDIVFVLVHARRRWVYFMAKSELYKHKFSAWFLNKMGAFPIKRGQSDVRSIKKAVT
                     LLKEGKLVCIFPQGTRQKTNNPLPPRRGVAMVAALTGAKVIPVFIENKAKVYKKTSVY
                     IGEPIDTGLDRKSPKEEISQKSQELMDIIYNLPEVYRNK"
     gene            33087..35249
                     /locus_tag="PLQ68_03295"
     CDS             33087..35249
                     /locus_tag="PLQ68_03295"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: protein motif:HMM:NF000907.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-3-methylbut-2-enyl
                     diphosphate reductase/30S ribosomal protein S1"
                     /protein_id="HXK71709.1"
                     /translation="MSKNAGFCFGVSRAVKMAEEIVQKEDIKYSYGNVIHNQQVINEL
                     REQGLITVNDICEIPDGSAVLVRAHGVSSTFYNAAKEKKLKLYDATCPYVGNIHKRVQ
                     MMYEEGYKIIIVGSSMHPEVIGINGYCNNSAIILEDESELEKIEPDDKNKYCCVAQTT
                     LIKEKYEKITSELKKKFTLLEVFDTICSATSLRQSQAVKLASNVDIMIVIGDPTSSNT
                     RKLYEVCKEKCTRTYLVSSAMQAEDIDINYKEMVGITAGASTPDRIVREVKETMEDNA
                     KKGTQNTFEEMLEDSLVTLRNGQVVTGKVIGVNDKEVYLDIGFKIDGTISVEEFPVGK
                     DGRQVAVGDTVEAIVTRVSDRDGIVYLSKKKIDEKRKYEDIKDAFTTGDAIKGTVKEA
                     TSGGLILDINGLDAFMPISHISTQFQRNLQKFIGRKLKVRVIECDRRKRKLIVSRKVL
                     IEEENERMEKEVWSKLVVGDVVKGTVKSFSNFGAFIDLGGIDGLAHISELSWGKINHP
                     EDVLELGQEVDVIIKDIDKDKRKVSLAYRKEEDDPWYMAEEKFAPNTIITGKVVRILP
                     FGVFVEIAKDIDALVHISQISNKRLKSASDVLEEGQMVDVAIIDVDLENKKINASIKA
                     VQPLDPLPTQEEIDRENQREERRQQSIKERTEKFTRRVENAETAPTRDHEPRQSRKQR
                     EELPVSHEEVLSNKIGSLLADFKFDEETASEAPKKEKK"
     gene            35329..35481
                     /locus_tag="PLQ68_03300"
     CDS             35329..35481
                     /locus_tag="PLQ68_03300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK71710.1"
                     /translation="METINDMSKDTLLKILKSREVRIKELEKEILLYQKIIKELMEKA
                     ESNDKN"
     gene            35468..36865
                     /gene="murD"
                     /locus_tag="PLQ68_03305"
     CDS             35468..36865
                     /gene="murD"
                     /locus_tag="PLQ68_03305"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015617525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HXK71711.1"
                     /translation="MIKTDNFPSFDKYIENLKSKKVAVVGLGIGNIDLFRFLAENSVN
                     VTGFDKSNDFDQLKSDLTSYTNASFVFGSDYLDKLIGYDVIFKTQSMRRDLPQFKKET
                     DNGAILTTEMWEFIKYCKAKIIAVTGSSGKTTTTTLIGELLKRQGYKVWVGGNIGTPL
                     FHKLNDINNDDYVVLELASCQLQMFGNKSPNISVVTNITPNHLDFHDSYDEYIYSKSI
                     IFKYQTSDDMLIINDDNDITNSFKSQANGKVYAFSRQKTFDNEEGAYIEDNSIFVKLG
                     PSRDKVLDISDILVPGKHNIENFMAAILATYDLVDKKTISNIAKHFNGVEHRVEYVRT
                     VNGVKFYNDSIGTTPSRTVASLQSFDQKVILITGGYDKHIPYDIMGPLINEKVKGLIL
                     MGQTKDKIKQAYNDYSCTVPLTEADTLEDAVAKAYQMAQEDDIVIMSPASASFDMFKN
                     FEVKGNLFKKIVNSL"
     gene            36886..38355
                     /gene="glpK"
                     /locus_tag="PLQ68_03310"
     CDS             36886..38355
                     /gene="glpK"
                     /locus_tag="PLQ68_03310"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013787284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HXK71712.1"
                     /translation="MKKYIIALDQGTTSSRAVAFDKEGRNIGSINKEFTQFFPSPGWV
                     EHDPYDILQSQMGALFELLKQYSIKGDEIAAIGITNQRETVVVWDKLTGKPVYNAIVW
                     QCRRTADICEKLKDKGYEKLIKSKTGLVLDAYFSGTKIKWILDNVEGAKGKNLLCGTI
                     DTWLVYNLTKEKAHVTDMSNASRTMLFNINTLEWDDEILDIFEIKKEMLPKVVPSSHI
                     VGHMEIEGVNVPIAGIAGDQQAALFGQTCYDKGDVKNTYGTGCFILMNTAEKPVFSDK
                     GLITTIAWNCEEQTCYALEGSIFNAGSSVQWLRDQIGLVTTAAQTEDMALAVDDTNDV
                     YFVPAFTGLGTPYWDMYARGLLIGLTRGTNKNHIVRAVLESMAYQTMDVIDCMQQESG
                     VKLNTLRVDGGASANNFLMQFQSDMLDCEVVRPAIQETTALGAAFLAGLAVRFWKDTK
                     ELKRVIKQDRIFSPKDYFDREYRKKRWHDAIERSMNWSR"
     gene            complement(38359..39222)
                     /locus_tag="PLQ68_03315"
     CDS             complement(38359..39222)
                     /locus_tag="PLQ68_03315"
                     /inference="COORDINATES: protein motif:HMM:NF019128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HXK71713.1"
                     /translation="MRLRRIITSLLAIIILLSPLMSIDVSASTNQIFPTDTKYYITNS
                     PVIYNYKNVNFYNDKLIINGYLVVSVTEFLNYFGSYSYEWRNETKSVYITDGYNEYII
                     YAGTSYMLKNGIMLESPAISVIYNDKIYASLKVMCDAIGIKTMYDEVSDSILLTERTF
                     FFNESYTFEDVYWLSRIVYAESGHETYEGMVAVANVVLNRVKHEDFPDTIYGVIFDKA
                     GGTQFTPVAAGTVYNEPSDEAVLAAKAALNGYNNVAWSLFFFNPRLAKSTWIADCRTL
                     YGSIGNHDFYY"
     regulatory      39451..39535
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            39681..40379
                     /locus_tag="PLQ68_03320"
     CDS             39681..40379
                     /locus_tag="PLQ68_03320"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HXK71714.1"
                     /translation="MEIYYLPVKDRQLGITHSYISQLRINDPNMGVLLDNTFMPVAER
                     TVICEKITSWLINNLFSDINKFRNREVGAEWFSVYIPVRVLVKDDFLEELKNTANTMN
                     INLKDLCLCFNNKILYEDHTVITNIFKQMKEMGIKTMIMDFGDDFCPITRISSIMPDY
                     VLLSQSVTDSLLSSKEEKKITAISLYNFLKSINVEAITSSIKDDEMAEILPESFNLCT
                     GTYAGNYRKPRSVR"
     gene            40381..41124
                     /locus_tag="PLQ68_03325"
     CDS             40381..41124
                     /locus_tag="PLQ68_03325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK71715.1"
                     /translation="MRKDPSVTKIAYKVHKTSLLFRALTISLVAIVILLSVAYLVSAF
                     YDRYSSFTVSTSYSGSGSSGKGGSDSQGSSLLKLALSLSETPDFTEPTTRLDANAIEN
                     CTNISGYSIPENVDQINGQHNGANYIAYTFYLKNAGSEAVTYEYAVNMLNVTQGVDEA
                     VRIRLYVDGEQTTYAKTKTDGSGPEKDTVEFLTESVIVRKRRAYFKPEDITKFTVVIW
                     IEGDDPECTDDIIGGKFKVTMDITIVEGS"
BASE COUNT        14786 a         5693 c         8018 g        12614 t
ORIGIN      
        1 gaaaaatcaa tgtatttgca atcatagaag ttatgctata atatcattat tattacttcg
       61 gaggaattta atatatgaaa cttaattaca aaaggacgtt tctagtgggg cttgcttttt
      121 tgtcaataag catgttctgg cagatgtacg attttgttat accgcttata ctattaaata
      181 agttcggtat aagagtagat atcgctaact ccataatggc gctggataat gttttaggat
      241 tattcatgct ccctttattt ggtgttttaa gtgataaagt aagtacaaaa atcggtagaa
      301 gaatgccatt tatacttgta ggtaccgcag ttgcagcaat atttacagtt gtacttccta
      361 ttgctgctaa ttctaataat ctgttattat ttatgatttc attaggatta gtgcttttag
      421 ctatgtcaac atatagatct ccggctgtag ctttgatgcc agatgttaca gcaaaacctg
      481 taagatcgca aggaaacgcg gttattaatc ttatgggtac cttgggtggt actatagtgt
      541 tagcactgaa aatgtttata aaaccaggtc cggactggga ttatacaaca ttttttataa
      601 ctgtagcaac attgatggta ataagtgttt taatcttatt tataactgta agagaaaaca
      661 aataccgcaa ggaaatggaa gatataaatt atggtgatcc gattattgaa caaagaagcg
      721 atgatataac tgaacctaaa gctgagaagc ttccaaagga tgttatgaag agcatgattt
      781 tcttgcttgg ttcaatattc ctttgggtat ttggatataa tgcagttaca acagctttct
      841 cgcaatacat agtaaattat tttgctcttc ctgaaggact tgcagcagga tttttgatgg
      901 ttgctaccat atcgggtgtt attacattta ttccaatagc ttatttaaca gcaagaatcg
      961 gaagaaaaaa gacaattttg ggagctttga ctctgggtat gatattcttt gcaatagccg
     1021 ctatgataac agatttctcg ttttatacga attttctgtt tgtcggtgta ggtattgttg
     1081 caggaactgt aacagtaaat accttgccaa tggttcttga aatgagcaaa ggagctacaa
     1141 taggtaagtt tacaggttac tattacacat gtacaatgag tgctcagata cttacaccta
     1201 taatcattgg tagacttttc tttaaaatag gttatcaggt attatttcca tatgcagtgg
     1261 catttttagt actttcattt attgcaatgc tctttgtcaa acatggtgat agtaaaccgc
     1321 aagtaaagaa gaaaaagcta gaaacttttg acttggagga ctagtaatgg aatggttaat
     1381 catattggta atgctcataa ttatctggtt attaatgata tggccttcag actatatcaa
     1441 agatgataag aaaaaactga ggaaatatct atatgcgcat agaggtctgc ataataatga
     1501 aaaaagtatt ccagagaact cacttgctgc ttttgatgca gcatgtgagg ctggatatgg
     1561 aatggagtta gatgtccaat tttctaaaga tatgcagatt gtggtatttc atgatgatga
     1621 tttaaagagg atgtgcggtg ttgataaaaa agtttttgaa ctgacctatg aagagctttc
     1681 agaattatca cttgctggta cagagcataa aataccatta tttacagatg ttttaaaatt
     1741 agttgatgga agaactccta tcgtagtaga acttaaaaac tgtaagattt atgaaaaact
     1801 tgttatagaa acaaataaga tactaagaaa ttacaaaggc gattattgtg tagagtcttt
     1861 caatcctctt attgttcgaa aatatgcaaa gacgaatcct atggtattaa gaggactttt
     1921 aatgactaat catgtaaaag ctaaatctat aaatgtcgta ttaggattca tgattcagga
     1981 aatgcttttg aatttcatgg cacggcctca atttatagca tgcgatcata aatcattaaa
     2041 tatctgggga gttaatgtag ttaaatttat ctttagaccg gtgatggcgg cttggacagt
     2101 aagaaatgag gacgaatttg agaaggttaa aaattttgat atagtcatat ttgaagactt
     2161 tctaccagat ccatcacgac ctgagctagt acataacaaa taattgctta tttgtgtttc
     2221 ttgtgtaatt gtaagtataa gatatatatt gctatacaga tgtaaaaatt tgaatgtaat
     2281 tgaatttaaa tacgcaggat attctgtcag aaggcaggtg tcctgttttc tttaaggact
     2341 atgcactatt gacaaaaaat atccaatgca tataattaat ttaagcaatg aaccaaagga
     2401 gtagtatgta agacttcaca gagagaaagc gcaagctgaa atgctttcag gtaaattaca
     2461 tatgaaggtc gttggggagc taaacgtgaa gcaagcgtta attgctgtta agagtcccga
     2521 tttattgggg aaatcaggtg gtaccgcgga atatatcctt tcgtcctgaa gtaattcaga
     2581 atgggaggat ttttattatg gatacatttt tgatagtaat ttcaataatt ctatgcgtaa
     2641 taggagcatt ggtctctttt atgtctagat tcattgtaag gaaaatgaat atagcttctt
     2701 ctcaggttat aaaaggaatt gaagatgaaa aggcgataga atcgctaaaa gaacagaaag
     2761 ccattatgaa agtcaagctt gtaggagctg ctatatttat tccaggaatg attgttcttt
     2821 acataatact taagggaatg taaaaaatat agtttaggaa atttaatact tattattaaa
     2881 aatagattag aggtattgta atgaaaattt tagagacaaa atatgaccca tcagcaatag
     2941 aagacagatt gtacagtttt tgggagcaaa aaggatattt taaggcaaat cctgataact
     3001 caaagaaacc ttttactgta atgatgcctc ctcctaatat aactgggcag cttcacatgg
     3061 gtcatgctat agataatact atgcaggata tattaataag aatgaaaaga atgcagggat
     3121 tcaatgcttt gtggcttcca ggtactgatc atgcaagtat agctacagaa gcaaaaatag
     3181 ttgagagtat gagaaaagaa ggaattgaca agcatgatat tggcagagag gaattcttaa
     3241 aaagagcttg gtcatggaaa gaaaaatatg ggagcagaat tgttgagcag ttaaaaaaaa
     3301 ttggctcatc atgtgactgg agcagagaaa gattcaccat ggatgaaggc ttgtcaaaag
     3361 ctgtagtaaa agcatttatt aatctttatg agaaaggact tatatatcgt ggtgagaaga
     3421 taattaactg gtgtccggtg tgtgggactt ctatttcaaa tgccgaagtt gaatacgaag
     3481 agcaggacgg attcctctgg catataaaat atcctattga taacgaaaac ggatttgtta
     3541 ttgtcgctac aactcggccc gaaacaatga taggcgatac agcggttgca gttaatccaa
     3601 atgatgaaag gtataagcat cttgttggta agtcagtagt attgccgcta gtaaataaga
     3661 ggataccggt tattgctgat gaatatgttg aaatggattt tggtacggga gtcgttaaaa
     3721 ttactcctgc tcatgaccct aatgatttcg aagtaggatt aaggcataat cttccaataa
     3781 ttaatgcaat gaatgatgat gctaccatga acgaaaatgc taatcagtat gctggactag
     3841 acagatatga agctagaaaa gtaatggtaa aggacttgga aaaacaagga ttaatagata
     3901 aaattgaaaa acatactcat aatgtcggtt cttgttatag atgcgattca gttgttgaac
     3961 ccagggtttc tacacagtgg ttcgtaaaaa tggaaccttt agcaaaacct gctgtggaag
     4021 tggttaataa caatgaaaca gaatttgtac catcaagatt ttcaaagatt tatctaaact
     4081 ggatggaaga ttgtcatgat tggtgcatat ccagacaact ttggtggggg catagaatac
     4141 ctgcttatta ttgtgatgac tgtaatgaga ttatggttgc agagaataga ccggaaaaat
     4201 gtacaaaatg taatagcact aatattcatc aggatgaaga cactttggat acatggttca
     4261 gttctgcgct ttggccattt tcgactttag gctggcctga taagacagaa gatttaaaat
     4321 acttttatcc aacgaatgtt ctcgtaactg gatatgacat aatatttttc tgggtggcga
     4381 gaatgatttt ttctggtgtt gagtatacag gtgttactcc attttcgaaa gtattaattc
     4441 atgggctagt tcgtgatgaa cttggcagaa aaatgtcaaa atcattagga aatggtgttg
     4501 atcctcttga tataatatct cagtatggta ctgatgcttt aagattctct cttattactg
     4561 gaaactctcc aggtaacgat ttaagatttt cagataccaa agtagaagct ggtagaaatt
     4621 ttgctaacaa gatatggaat gcgacaagat ttgtgcttat gaatttcgat gagtatgttg
     4681 atttttctga tattaacaga gacaactttg aaaatcctga aaaatggata ctgacaagac
     4741 tcaatcaggt tattagagaa gttacagata atatggaaaa atttgaactt ggaatagctc
     4801 ttcagaagat ttatgaattt atttgggatg agttctgtga ttggtatatt gaacttgtga
     4861 aaccaaaact ttatgacaat cagaattcaa aaagaaaaga agctttatat gttttaaatt
     4921 atattttaac aaatgcgatg aaacttctgc atccttttat gccatttata tcagaagaga
     4981 tttatcagag ccttgcagat tctgaagagt caattatgat atcaagctgg ccttcagtgg
     5041 aaatgatgaa tgaatatcct gaatacgctg aggatatgga tgtgataatg aattctatta
     5101 agagcataag gaataaaaga gctgagctga atattgatgt taagatgaaa tccaatatat
     5161 tttttgtatc tgaggatgaa catactcaga atttattgca gcaaggggaa cagtacttta
     5221 taaaactcgc ttctgcaaaa gctgtttcgg tattcaaaaa cgaaaaagat atacctgata
     5281 attcaatttc tatgattctt cctggattta agatatttat gccttttaag gaattagttg
     5341 acattaaaga agagattcta aggcttgaaa aggaatatga aaaagctaca tcggaaatgg
     5401 aaagagcaaa aagcaaactg gaaaatgaag cttttgtatc aaaagctcct tctaatcttg
     5461 tagaagctga aaaacagaaa cttaagaaat ttgaggattt agcacaagac attaaaaata
     5521 gtctggagaa cttaaaatgc atgtgattga tgcgcattgt gatactttac tagcaatgtg
     5581 cgaaaaaaaa gaaaatttgc aatactccta tgaattggtc aaaactcttc gatttcatta
     5641 tatgcagttt tttgcaatgt tctgtccgcc atcactatta aaagaagaag atggatttgc
     5701 taaatctgaa aagcttcttc atatgatgat gaatctttac cagaaaatga cagagcaata
     5761 ttcatacaat aaagtcttgt gtaaaaatga tatagttgat ttcgagaaaa agaaggatga
     5821 taaaaatcct tctttctttt ctttattgtc agcagaagga gtctatcttt caaaaaatga
     5881 catatcatat atagattatt tgtataaaaa caatgtaagg tgtctgtcct ttacatggaa
     5941 tccttcgaat gaatttggtc aaggcgtaaa tggtgacagc aaaaaaggat taagtaatct
     6001 tggtataaga gccgtctcaa ggtgtaatga acttggtatt cttgttgacg tctcccacgc
     6061 taacgatagg acttttcatg atattgcatc aacatcaaaa aaaccttttg tagctactca
     6121 ttcaaatagc agatatgttt gtgctcacaa aagaaatatg gatgatgaca tgttaagaac
     6181 acttgcaaaa gctcaaggag ttacaggcat aaactatttt agtgattttc ttgtggaaaa
     6241 agaaaaaaat aaaacagctg atttttttga tataattaag catatagagt atatagcagc
     6301 tttaatagga gtaaattata tagggttagg atctgatttt gacgggatag acaaagcagc
     6361 tatagatgat gttacaaagg ttccagatat cataaatatg cttttaaaac tcaattacaa
     6421 ggaagatgaa gtcaataata tctgttcagg taattttata cgagtactaa aggaggtttt
     6481 gcatgatgaa tgaaataacg ccaatagaat tcagggataa taagttaata ttaatagatc
     6541 agagaaaatt atttgattca gaagaatttt ttgtatgcga ttcaatagaa tcggtttatt
     6601 tctctattaa agaaatggta gttagaggag cgcctgcaat aggagtaaca gcagggtatg
     6661 gtatgtatat aaaagcgtta tctataaaaa ctgcagattt agacgaattc agaattgaga
     6721 tgaaaaaagc agcagagtat ctagattctt caagaccgac ggctgttaat ctttcatatg
     6781 ctacaaaaag gattatgtcg gtagttaacg aaaactggga tgaagttggc gaactgaaaa
     6841 atgcacttta taaagaagct attgcaatac atcaagaaga tattgatgct aacaggaaaa
     6901 ttggtgagaa tatgctttct cttcttaaaa atgcaaaaag agtacttaca gtgtgtaatg
     6961 caggtatttt agcgacaact tcatatggta cagctacatc agcaatgtac ttagctaagg
     7021 agcagggcaa ggaacttata gtttatgcat gtgaaactcg tcctcgactt caaggagcaa
     7081 gacttacagc ttatgagctt aataagaatg gtattgatgt aactgttata actgacggga
     7141 tggttgcaca tcttatgagt aaaaatatgg ttgatgctgt aattgtcgga tgtgacaggg
     7201 tagcatcaaa tggggataca gcaaataaga taggaacaaa gaatatatcc ataattgcaa
     7261 agagatacaa tattcctatg tatatagcat gtcctacttc tactattgat atggatatag
     7321 aagacggatc aaagattgtt attgaacaga gggattctgt ggaagtcaca catataaacg
     7381 gtgtcagaat agtgccgcct ggggttaaag tttttaatcc atcttttgat gttactgata
     7441 atgcagatat aacagcaata gttacagaaa agggtatagc taatcctccg tataacgtta
     7501 cccttaaaat actataacta aatttaagta ctatttatat ttgtattaga atttagtcat
     7561 ttatacttaa aatattgata ataattgtat tttaataata atatttcttg ccaactataa
     7621 actttttgtg aactttgata tttgatgttg aaactatagt gaacctctgc tatagtatac
     7681 ccgttgcagc ctttaaggct gcattatgcc agtaaaaagt cataaggagg atatactatg
     7741 acagggtaca actgggaaaa gattataaaa gatttcgacg ctaattcaag catctacgat
     7801 tctattaaag acaatacaga tgaatttata gactcttaca aagaccatgt tgaatggaaa
     7861 gcaggatggg ggcatgattt tcattgcgat tactgctcag gcaggctaat atttgacaag
     7921 acaaacagag gtaagtatac ttgtaaagta tgtggcaaag agaatacagg cgacagaaaa
     7981 gactggtcct ggacaacatc ttacagaagt gccagctgta cgcatgtaaa acatgctggt
     8041 attttataca gtattacaaa acagcagaaa tacattgatt acataagaaa agtcatgaac
     8101 tttcttacag ataattatga taagtttgaa tcatttgctc ctaatcttcg ttttttagga
     8161 aggtatactg gaacaggatt atctgatgcg cataatacaa tgtcattact atacggtata
     8221 gatgctgcac aggatcatct tactaaagct gaactagata aatggcataa gggatttttt
     8281 actccacagg cagatatgtt tgatttcttt tcaaataaga tatacaacat acctctatgg
     8341 caaaaagtca gtgaagccaa tataggcgtt ttctttaaag atgaaactat aataaaaaga
     8401 gctttttact caaaatttgg tatattggat cagttaagaa gaggtgttac agaagatggc
     8461 atgtggtttg agacaagtac aggatatcat ttcttcggtc tggctgctac agctgaactt
     8521 ctattattgt gtcggagaaa tgaggtatta tgcatacctg aacatcagga actgatggat
     8581 atcattgaga gccaattcat gtttccactt aaaatcatgt tcaagaacgg ctgtattgca
     8641 aatccttgtg acagtggact ggatatggac atttttactt atcagaatgc atatgttaaa
     8701 gcctgtgctt tgtttgacaa cccatatttc aagcaggttt taggctacat aaaacatata
     8761 aaaaatcaac ctagaacatt ggatgaaatt ctttatagta tagaatatga tgagtgtgat
     8821 catcttcctg acttcgggtc gaataatttc gaagattcat tttgtgcaat attaaaaaat
     8881 gatgtgactg aattgtatct taagacagga ataaagacga tctgtcatgc tcatcctgat
     8941 gtaatgacag ttgaaatggc attttatgga gatctgttgt caaaagatat tggctcttca
     9001 ggatatagtt caaagatttt taatgagtgg caacataaga caattgctca taatacaata
     9061 accattaata gacaagatca gacatacact cctgttggtg aaggcatctg gcctgaggga
     9121 attgttgagt attatgatga acagcatata agagcaaaat ctaaaaatgt gtacgaatgt
     9181 gttgattata cccgtgatat gaaagtaaaa gagaataaga tatatgacca gtttagcgta
     9241 aaagcagttg aagatttaga gcttgattac gcattttatt caaagggtga atgtatcatt
     9301 gagaatgaat atgaaaaagt cgattcaatc ggtgaatctg aaggatatca gcacttattt
     9361 gatataaaga agattgataa cagagatact gtaactgtaa aatttgagtt ggaagataaa
     9421 attttaagtg ttactattga gcctgatgaa gattcagttg tatacatagt aaacagttat
     9481 gttaataatt tcaatgacac aagatatggt ataattataa gaagaaatgg taaagaaaga
     9541 atatttaatg tgacatatga atgtgtcatg aaataggatt ttaatgcata cttatgaaac
     9601 gcatattatt aaagaaagca tgtgtgactg cttcagaaga ttaaaactgc agcatatgtt
     9661 cataatgatg caggatgcag catacaaaga tcttctcaca tatgggtgtg gaagaaaatc
     9721 attactcgat aaagatgttg cttttcttct tagaaggtca gtggtagaag taataaatgc
     9781 tccaagagct gatgatactg ttgttgtagg aacatcacag atctctttgc aggggatgac
     9841 ttatacaaga gatttcatat tcatatgtaa tgatgagata tgtgtgaagg cttcaactca
     9901 gtggttttta tgtaaaattt ccgataaatc actaattcgt ccgactattc agacaatatc
     9961 atactctggt gactcagttg gtatatccat tgataataaa cctgaaatac cagatgacag
    10021 aggagaaata tataatacta taaaaatcgg ttacagcgct attgacggaa atatgcatgt
    10081 caataatact gagtatgttt catgggtgtg tgatgctcct aatgctatca gttatttaga
    10141 agagggccat tattcttttg atatatactt taaaaaagag gtaatacctc atgatactgt
    10201 agacttatat cagattaata atgtagttta tggtatatcc caaaaccaaa ctagctttat
    10261 tgcaaaattt aaataataaa tctgttgtat tgttgtatta atgaactcgg ataaaactgg
    10321 gttcttttta cgtgaattat ttgataaaaa gaacaaatgt ttttatttct agacaactta
    10381 agagaaatat taatattatt aaggttaaaa ttaaataaat taatatttag tgttgacaat
    10441 tcaaaaaata ctttgtatta tacttttgta actgataaag ataagactgg atgctttttg
    10501 ataatataac taggaggacg agtaaatgaa gaattttgta ccatcgcaat gtcctatatg
    10561 tgaaggagag cttgtaataa ctgatatgga atgtaaaaac tgtcgaactc atatcagtgg
    10621 agagttttat acaacaaaac ttgcaaaact agatccgaac caacttgagt tcattgaagc
    10681 atttataaaa tgcagaggaa gcataaagga aatggagaag gaattgaaga tatcttatcc
    10741 tacagtaaaa tcccgtcttg agataattgc aaaaactatg ggatacaagg atgagaagga
    10801 agaggatgac accagaatga aggtgcttga tagcattgaa aaaggtgaga tgactgttga
    10861 agaagcatta aaaatactta aataggggtg tgtaactatg aatgaatcaa aagaaataga
    10921 aatgatttta aaacttcttg aagaaaagaa aataacgact gaagaagcta aaatgcttat
    10981 agaagctttg aaaacagcta ataacaatca aagtgaagat caagatacct atgagaacga
    11041 tgataaaagt ggttctgaaa atgtattttg tgatgatttt gatgatgtca gaacaaaatt
    11101 tagtaatttt ggttttgata taaaagatac aatattagat aagatgtctg aaattaagag
    11161 taatgttttt gattcattat catttgacat cccgagtaat actgacttat ttgacttaaa
    11221 gtcagataag atggattttg aacttaatga taaagcttca ataagtattg agggaaccaa
    11281 tagttctgta atagtaaaac atggttcagg tgataaggtg ctgattaatt tttctgtaaa
    11341 atcaagagat tacgatagaa tacatgaaat tatagaaata caatactcaa aagatttctt
    11401 aggaattact gttgatgatg catataagga aagaatagtc attacaattg agcttcctga
    11461 tattgattta cttaaattaa aaattgaaac tactaatgga cctattagtc tttcagatgt
    11521 taaatgcgga caaattcatc ttgaaggtac aaatggctca ataagcctta gaagatgtac
    11581 cggaaatgct ttcaaagcag aaacaagcaa tggttctgtt cgttgtgaac aaaatagttt
    11641 tgatgagtca attattgttg aatcatcaaa tggagctgtt tactcaaata tggataattg
    11701 taaacttatt gatatggaaa ctacaaatgg ttctattaaa ttttacggca gttgtgcaag
    11761 aagctttaaa gcagaaacaa caaatggctc agtaattttc gacagactaa acccatatca
    11821 gtcagaaagc aaatttcttt tgatgggtga gactactaac ggttcagtta cagcatatat
    11881 caatcgtaat tatggagttg tatttgaatg ttcttccgga aggcatggca atataaggct
    11941 tggaagaaat ttcaatatgc agtctatgca gagaaacagt attggaagaa tttcttatgc
    12001 taaaggcgag agcaggtatg cttataaatc tgatattgtt gcagcggttg aaacaaagac
    12061 aagcaacgga actataatta tggaatttgt gtgagggata ttatgaaaag gggaacaatg
    12121 gtaagatttt actatccaga ctatatttca gaagataatg cagaagttaa tgtatcacaa
    12181 aaaattaact acagaccatc ttcttcagtt tggacaaata atcagtttta ttccaagtat
    12241 ttaaaatagt ggtattatag tgttgaatta ttcaactatt tggtaataat acaattaggt
    12301 gagtgcacta taattatgtg aaaatatcaa aaatatattg cattattata tattttcata
    12361 tataatccaa attaatagca aaaaatggca taaaggaaga gaaatggata aaaaatcttc
    12421 aaaaaaacag aagaccaagg catctgatgt tatcaaaatg atggtaagaa tatataaaga
    12481 gacatcagaa cacaaaaaat ggggtattgc aagatgggta ggcggattac tttcaggagc
    12541 agttgctgta tatgagtatt attttattgg atcagccctg gatgttgctt tatctaatga
    12601 ttataaaagg cttttatcat ttatatatgc tttagtagga atatttgttt ttaggattat
    12661 tttacagttt acaaatccgc ttattgacgg gcaatacgaa ctacgttcat atagaacgct
    12721 ttcgttaaat gcatatagaa agattgataa gcttcagatg ggatattatg agaatgtgca
    12781 tactgctgat acgataagca cactgattga tgatattgaa aaaataaaaa cttttatggg
    12841 aaatagtata gccggattat tatcatggaa tcctataact ataatattat cagtaataat
    12901 tctatgcact attaactgga aacttaccat attctcgcta attatagtgc cagtagttat
    12961 gctagtgctt aacagtgtat caaaaccatt aaaagatacc aaaaataaca tacaggaata
    13021 tactgctgtg ttcaattctc atcttcgtga ttttattgaa ggaaacgata tatataaagc
    13081 atttaacatg catagaaaac atacagagaa atttgaggaa tcttgccaga agattgcaaa
    13141 cgagtcatat aaaagcagcc gtgttatggc taaaaacagg gcattgaata ttttaatgat
    13201 ggtaatacct caaggaattt gtatgtttgc atcaatattc tttattgcca gaaatgagct
    13261 gactatcgga caatatgtca tattcagtaa tatgctgtgg cctttactta gtgcattcag
    13321 aacaacaggt atagcctgga gcgaaatggt agggtattca ggtacagcac agagatattt
    13381 taaattttta gaatataacg aagagagaac tggcggagaa gatttcggtg ttaatgattc
    13441 agagataatt gttgaattta acaatgtgag tttttcatat catgataata tacctctttt
    13501 aagaaatgtg acttttaagt tatacagaaa ttcaagactc gcattatgtg gaataagtgg
    13561 aagcggtaaa agcacaattt ttaaattaat atgtggttac tatgagaatt attcagggga
    13621 tataatagtt tttggtcata atatccgtga ttggaatctt aatgctctta gaaaatacat
    13681 gacatgcgtg acacaggatg tatttctttt tgatgatagc attgtcaata atataaggat
    13741 gggaaaatta gatgctacag atgagatgat tgaagaagct tcaaaaaaag catatataga
    13801 ccagtttata aatgatgttg gagaaagagg cggaaaagtt tcaggaggac aaaaacagag
    13861 gattgcagtt gccagggctt tattaaaaga tgctcctttg ataatgcttg atgagcccac
    13921 aagcgctttg gacacaaaag cagaatatta tgtacaacaa tcaatagaaa acttaaaaca
    13981 gggacgatct gttcttgtaa tagcacatag gcttacaaca attattgatt ctgacataat
    14041 tattgtactt gaagagggta agataattga acagggaagt catgatcagc ttattgacag
    14101 gaatggaaga tattcagaac tctatacaag acaacttata gagcaggggg tataacatga
    14161 ttaaagatat gaaattcata ttaatgctta taggaaagaa taaagctaaa tattttactt
    14221 tattaccatt atggtgcata ataggcagtc ttgttgatat ttcactatca tatgcgctta
    14281 agcttctaac tgactacttt atgtataaga attctcagaa tattacaaat ctgattataa
    14341 tatttagtgc tacgattatc accagcatac ttttctgtat tatttttcat cttcataaca
    14401 aagttaagga agatatactt agagatataa gaataatgac attcaaagaa gtacagaaat
    14461 taccttcatc atactttaaa agcacgcaca gcggtgatct tgtatcaaaa tttaatgcag
    14521 atactcagag cgtaacaact gtattcggtg aatttgaaaa tatttttgaa tcaataatcg
    14581 caatgtttat aagaatacct tttgtgctct tactcgatgt cagatttgga gcaatgttgt
    14641 taggattctc agctgtaaca ggtatactta atgccttgtt cataaaacct ttaagagaaa
    14701 agcataaagt catactggaa aataaagctg ttttaagtaa ggaagcatct gaagctgtaa
    14761 caggatttac agctgtaaaa atgctttctt taagttcgca tcttttgaaa aaattcacta
    14821 atagcgtaca agataatttt aacagcgaat ggaaagctaa aaaaacttat acattacaaa
    14881 gaggtcttaa taatatcatt tggaactcat catatctgtt aacaactata ctaggatgta
    14941 tatttgttat caaaggtact ttagaaccag ggaactattt ggcaatatct acaggtaatg
    15001 gaattggatg gtccatatca gttttaatat cagctcttcc aaatttacag aaagcatttg
    15061 caggatcaaa caggcttaag acactgtatg atgcacaaaa agaacctgat agatatcaga
    15121 tagatggaac tgagtctgat atgggtttgg atgtacagga tttagagttt tcatatgaaa
    15181 tggaaaaaga gaataataag aataatgaga ataatgagaa taacaatact ttagataatg
    15241 aagaatcgga ctacacatca tatcagttta agatatcagg attaagtttt agtatcaaca
    15301 aaggagatac attagcgctt gttggagata gcggaggagg gaaaagcaca attgcaaaat
    15361 tgcttttagg attatacatg attaatagcg gctcaataca ggtcaatgga caggcatata
    15421 aagattactc attagaagag ttgcgtgatc agatgggata tgttcctcag gatgcatata
    15481 tatttaatgg tactattaga gaaaatatct tatatgggaa tactgatgct gatgaacagc
    15541 agataattaa cgcagctata ttagcaaatg cacatgattt tatattggct caacctgatg
    15601 gatatgaaac atatgtcggt gaaaggggta taaggttatc tggaggtcag cgtcagagaa
    15661 tagctatagc aagagcaatt attaaaaatc cttctatact gcttcttgat gaagcaactt
    15721 catcactaga tagcgaatca gaaaagctta ttcaggatgc tctggacaga tttatgcata
    15781 acaagacatc agttgtaata gctcataggt tatcaacaat tttaaatgca gataaaatct
    15841 gttatattaa agatggtaat gtagtagaag agggcactca taatgagtta ttgagtaaaa
    15901 aaggatacta ccacagccta tattatcgcg aatttgcaaa taaaactaat gaatcgtaat
    15961 ggttaatgca atagcatcag ctagctgatt agtcaaagct ggcccaggat gagcataata
    16021 agccggataa taggcagtct tgtaactagc atctttgaaa gtagtatcaa ggctgctgtt
    16081 attgtctcta gtagcagtta tgtagtatct gattgtatca ttactttttg aacctgtaat
    16141 ttgttttaca ttgaaagaat acttaaaatt atctgaatat tcatgaatgt ttttactttt
    16201 ccaggttcca ttattaactg aataaaagaa agtaatatat tctaatggga aacgtccata
    16261 tccagcataa taatcagtat tttctgtatc agaagaaaca tatgctgtaa tagtaaatat
    16321 attagtttct gtattaaaat ctggataact gaatacatca agatcagacc cattcttcca
    16381 gtttaataca tctttgtata gatcataaat ctgataatga agggttctat cctcgtttac
    16441 tcttcctatg tgaagatggg aatcatatct tttatattca tctattgtag ctatcggtat
    16501 gcaagtatat acaaaatcgc cttcatcaat accatcaata ggtataacat gaagatattc
    16561 aatatataca ggctctgtgt aacctatttc agatttaact acgatatggc cagtttgtga
    16621 tgaaatatct tttttaatat atattatctc tccatcgtat attgggtata tatgttcacc
    16681 tacatttata gttaaatctg ttccctgatg tggatttgta cctgttacaa atcttggttg
    16741 attattcttt gaggtacata atggccagta ctcaattcct tgggtatttt tatatgggga
    16801 aagagcttgt aatgacaagt tagatatttc cttttgtaca taagctctgt cataatcaga
    16861 ggctgcagtt attgttaaaa aagataatac tagaataagt attatgtaat gagtaatttt
    16921 agtacgaaag catatacgtt ttttcattac tacctccttc atatccgctt aatataaatg
    16981 ttttgctatt tttccaatga gcgtatacaa ctaagaaaga ttcgcttact acgaaagttt
    17041 tgagaatttc attagactgt aagtcaagaa atgttacagt tacatttttg ttatcaagaa
    17101 gaactacaga tgctacattc aaagtatctg tcctcatagc tgatattttc agtgcagagg
    17161 attgtgatcc gagttttcct ttttcaacat tcaaagtaag taaattaagt atttcatatt
    17221 cttgataata tgtttttgtg tatatgtagt agggataact gaagtttcca tttgatgata
    17281 tagaatatga gacaggagat ttgatttttg atgcaatatc tactcgccat aacatgtcat
    17341 tacaagtata aaatatagtt gtttccgaaa tatatgcatc attatagaaa gagtcttcta
    17401 ctaaaaggat atcttctttt gtttcaaaat cataaagcct tatatcgttg gtctttccgt
    17461 tatttcttct gttagtgaga tagatgagat attttccatt ttcagatatt ttaggttttg
    17521 ttccccagta aagaccattt gcttcgtttt cctctagtga tttatcatac gtcgtatcat
    17581 catcttcttc aagtaataaa gtatccataa tctgcttaga aagattaaca taatatattt
    17641 tatgctgatt ccataatata tataacgagt ctgatgaatt tggaatagct attaatgatg
    17701 tatttagagt atcatttgat cttgattgat aaattggata gagtctgttt tcaaaatcca
    17761 caacaggtat tgaaacagtg tatccatcag tagcagtcaa tgtgagatta ttatagtcat
    17821 atgttaaagg cattgactga atcccgtttt gatactgcat aatcatatca aattccataa
    17881 aatctggttc ttcttcaatt ggttcttctg aaggctgaat gggagactgc gtaagctgat
    17941 ttgacggtgt atcagtaata gattgcgttg gttctgcacc gttgcatgaa gcaaatagaa
    18001 tagccatggt tagaataacc agcattaata cgatattttg ccttgatttt cttaagaatt
    18061 ccttaatcat atatatattc tatctttata agcctcaaat aacaataata aatgttgtta
    18121 ggaaacacaa tgaaatgata taatcaatat gctacaaaag ataaggaggt tattaccttg
    18181 aaaagaagaa atttatctgt aatcttatta attgctttgt ttattatgat gttaccagta
    18241 actgcttcag atgattcata taaccgagat caggtttcat caatttattc tacaacaaca
    18301 gttgataatc taaaggctac cagcgctatt ttggttgaag tgaacaccgg tcagattcta
    18361 tattcaaagg atgaagataa gcaaataccc atagcaagcc ttacaaaaat tatgaccgtt
    18421 tatcttgtaa tggaagcgat tgcgaaaaat gagatttctt atgacaccat gattaaagca
    18481 tcagctagcg ctcatggtgt agaaggttca tcagtttatc tgaatttagg tgaagaattt
    18541 actcttagag aaatgcttta tgcaatagaa gtgcgttcgg ctaatgatgc cgctgttgca
    18601 gtagctgaag ccatttcagg aacgcagggt gaatttgtta acagaatgaa taacaaagct
    18661 acagaattag gtatgtttaa tacatatttc aaagactgta caggtcttac tgatgcaggt
    18721 cattacagta cagtcaaaga catttccatt atcgcaacaa gacttgtatt agaatatccg
    18781 gatatatttg aattcagccg tacacttgaa aaggactttc gagataaaga gcacagagat
    18841 tatcagtata tggttaatag aaataattta ttgaactatt acgatggtgc tacaggactt
    18901 aagacaggat ttactactgc tgcaggatac tgtcttgtcg gatctgctca gaaagcagat
    18961 atttcactcg tagctatagt tacaggggag ccggataata atcacagagt agctgaaaca
    19021 gcatttctac tggattatgg attttcaaat tttatgtata ccaagacaga cgatgaccaa
    19081 aaaatggtag gaacgataga ggttagaaaa ggtatagata aaactgtgga gacatatgca
    19141 gatggaacgc ttacattatt catgaagaaa tcagatatat ctagaatatc aaaagaaatt
    19201 gtttatgata tggctacttt agaagcacct gttgaaaaag atacagtaat aggaaaagtt
    19261 gtgtacagtc tggatgagga tatcttagga gaagtatctg ttaaaacttc tcaaagcatg
    19321 gaaaaagcag gattctggtc actattatgg agatcagtat tatcatggtt cggcattgat
    19381 tggcagaaat gagttttcgg ctttacatgt cataccttaa aatatataat taacggaagg
    19441 aaaaggatgt aaagcttatg ttacaaagta ttattgactc tatgatacct atgctatata
    19501 tgataccggt tatgctgata tctctgactt tgcatgaatg tgcacatggt ctaatggctt
    19561 tgaaattagg agatacgact gcaagagatg caggaagatt aactcttaat cctttaaagc
    19621 atctcgatcc tgttggagca gtgatgatgg taatagcccg ggtaggatgg gcaaagccag
    19681 ttcctgttaa ccctatgaat tttaaagatc ctaaaaacgg gatggctttg gtgggtcttg
    19741 ctggaccact tacaaatctg attgttgctt ttggtgctgc ttttccagta gcccttatag
    19801 aaaatgctgt ttatctacat ggtgtcaaac ttactacttt tcttgaaatt gcatatgtat
    19861 tctgctatct tttatttcag gctaatatat tccttgctat ttttaacctt atacctatac
    19921 cgccatttga tggttcaagg ataatgttcg gaatactgcc tgacagatat tatttcagcg
    19981 ttatgagata tgagagaatt gtcggaatgg cttttatatt tatattcttc atattcagat
    20041 ctcagtttgc tgttgtactg gattttataa gttcgccaat aacgaaatca tttttatttg
    20101 ttgcgaactg gttcgtggga ctattttact aggaggattt tatgaaaaac atggttgtat
    20161 taagtggaac aagaagcacc gggtcgcttc atttgggtaa ttatttcgga gcagttgaaa
    20221 actgggtaaa tttacaaaat aaatatgact gctatttctt tattgcagac tggcattctc
    20281 ttacaactgg ttatgaagat accaataact atgtatacaa tataaatgaa gttgttatgg
    20341 atttatatgc atcaggtctt gatgctgata aatgtaatat atttttacag tcaaaaatac
    20401 aaaatcatgc cgagcttcat cttcttttat ccatgataac cccattatcc tggcttgaaa
    20461 gatgtcccac atataaagac cagctagcgc agcttagtga gaaggatata aggacatatg
    20521 ggtttttagg gtatccttgt ttaatggcat cagatattct catatacaga gctgatgctg
    20581 tgcctgtagg tgaagatcag cttcctcatc ttgaagtaac aagggagata gcaagaagat
    20641 tcaatcatat ttatactgaa gtatttcctg aaccaaaagc tctgttaaca aaatcaaagg
    20701 ttttacctgg acttgatggc agaaagatga gtaaaagtta tgataatacg attcttctaa
    20761 aagataaaga tgatgtgtta agaactaaaa ttatgagcat gataaccgat cctgcaagaa
    20821 tcaagaaaac tgatccaggt catcctgatg tatgttcagt ttttgcattt cataaagtat
    20881 ttaatgaaaa tgaagtacct tcaatctgcg atgcatgtaa aaaaggtgaa atcggatgtg
    20941 ttgcatgtaa aaagaacctt taccagaagg ttactgaatt tatgaagcca attcatgaaa
    21001 gaagaagcga aattgaatca aagcctgaac ttattaagaa gatacttgaa aaagggaacg
    21061 agaatgctaa gataaagaca gacgagacaa tgaaattggt tagaaaggcc atgaagataa
    21121 attgggatta ggatatcaga ttaaacttga aaattttgaa ggacctctgg atttgttgct
    21181 tcatcttatt gatgagaata agatgagtat ttatgatatt aaaattagta taattacaga
    21241 gcagtatctg gattacctta tggctatgca acagcttgat atggaaatag ccagtgattt
    21301 tctagttatt gcggcaactt tgctacatat aaaatccaaa tcaatgcttc caaaaaccaa
    21361 agatgaagca gtagactctg aaactgatct tattaaacag cttattgaat acaaaaagat
    21421 aaaaatagca ggggaaagat ttaaagaaat gcatgaatca gggcagaaaa gttttcatgc
    21481 ttttgcttca aatgaagatt ttggagaatc tgaaaaaaca tatatgctat caaaaagcct
    21541 tttagctatg atgtatcgcg aatcaatgca aagatcagtt gagaaaacca ataagaaggc
    21601 tataaatatt aaagaaatag taaaaaaaga aaaagtatcg cttgccaagt ctataaggaa
    21661 agttattgat gtcttattta aagataaaag gataagtttc tttcagaggt ttaagattca
    21721 ttccttatcc aaagctgacc ttgtaacgga ttttttatct atactagttt tatcttcaca
    21781 aagaaaagcg atactaaagc agaaaaggcc ttttgaagac attatggttg aaggaacaga
    21841 aaatttaagt tatccggaca atgttcatga aatatacgat tgggagtaat taatatgaat
    21901 aaagattatg tcattaattt aataaatgca atgctgtatg ctaatggtga gccgattact
    21961 ttatcaagat ttcttcagat acttgaaatg gaaaaagagg atttttttga gataatgaat
    22021 gagcatattg agaatcttaa aaaatccaaa tcaggggtcc ttataagaag ggttgaagat
    22081 gcatatcaaa tgtgtactaa tccgttatat tttactacag ttagacaatt gtttgaaaaa
    22141 aagaagacag agaccctgtc aagagctgca tatgagactt tggctataat cgcatacaat
    22201 aaagaagcta caagagcaaa aatagaagct ataagaggta attcagacag tcctttaaaa
    22261 acattgcttg aaagaggcct tataatagaa gccggaagac tggaagctcc tggaagacct
    22321 gttttatata aaccagcaga ggagttctac agagcttttg atgtttctgc tcttgatgag
    22381 cttatcccct tagataattt tgatgaagag cctgaagagg agtcttcaga aatattagat
    22441 aatcaaaaat ttactgaaca ggattctatt taaaataatc tgtaagtttt ccttggaatg
    22501 tagccattcc tatctgagaa aaacttgttt taccatatcc tccatatgac tcgctaactg
    22561 ctttgtctga tgcttcaata tcatcttgtg ttattctttt gttatctgta cctgtgcctt
    22621 cgtaccaaag ataatatgta tctttgtgtt tatatacagt agcaaaccac aatgctcctt
    22681 catcccagga acctcgattt cctcttctta taattgtatc ggaacttttt tcccagttta
    22741 gaagatcagt cgaccttgat atgccaatgc tttcagggta atctttaaag cgttcattat
    22801 ttccaaaaaa actataatac ataccatctt cgtagaaaat tctgtttgta gacaaactgt
    22861 gactgagttc gtccggttcg aaaactactc tttctttgga tatatcaaag tcaataccat
    22921 ttgtactcgg acatacataa aattttgata caccctttga accagctgtc catctttgat
    22981 aaaaaagata aatcataccg tcttttataa caacttcagg aactactgca ttgtgtatta
    23041 ttggattatt cttatacttt tcaaaatgta tgccatcttc tgatattgct aaagatacac
    23101 tatgcggcat ttccagtgaa tgtgctgaat agtaaagata taccttttta tctatcacta
    23161 ccgctgcagg atcaagtatg tgattagagt catacgcttt tgcatcagag tttactttta
    23221 gtattggttc tttttcaata tattcccatg taactccgtt aaatgcttca aaagaacttg
    23281 tagcaacacc catctggtcg tgattgttat gatcctggcc tcgaaaatag aaaaaaagct
    23341 tattgtcaaa ttccagaata tctggatttg cagcatactt gctatagaat gtaccaggtg
    23401 ttctttttat tacaggatta tttatatatt tattaaacat ttcagcctcc aaacaattta
    23461 ttgactatat tgatatctta gaataagaaa aagactaagt aaacatctat tttattaatt
    23521 cagtcagcta gatatacaaa tcattgcatg catattaaaa tagatgtaga atttaataaa
    23581 ggctcacagc ggggaatgca tggaattaac aaatgaacaa aaaaagcaga tagaacagct
    23641 tcagcaggat aataacaaag atgttatatc cggctatctt gctattatta cagctacagt
    23701 accattagtt atatggccgt tttttcctca aagatttttc tttcctaaaa tggtgatgct
    23761 ttatgcattt gctcttctga tgttggtctt tttggctgcc ttaaaattaa acatattttc
    23821 aaaaagatta ttcaaagaac cagctgtgta ctctgtgttg tttttcattg tatacactac
    23881 agtaactttg ttcagttcca ttaatatgga tctttcgctt tatggagctc atgaaagaaa
    23941 agaaacttac tttgtattag taatatatgt actgtttttt cttgtagctg caaatcatta
    24001 tagatataag aaatggcatg ttatagcttt tggaataatg atagggttgc tttgcatata
    24061 tgcaatattc cagttatatg gacttgaact tatagattta acttttctat ctaaatacaa
    24121 aatttcagct tcatggcatt atatagcttt ctccacattg ggaaatccta actttttggc
    24181 tagtatgctt gcaatgtctt tgccgatttt tgtatttgca tttattaagt ccggcaacta
    24241 tttctggaca ataccaatgt ctttgaatta tttcgtatta ttgacaacca agacaagagg
    24301 cgcatggatg gcagcattcg tgtcatttac tgttttggtt gtgttgatgg ttataaagaa
    24361 gtacagtatc aaaaatatta taatatgctg tgttatacat atagggctta ctgttctgta
    24421 tgcattactg ttttccggtt ttctagatag aattgtaagt gctgttgata ccgttgtcac
    24481 tgttgtcgct tcagataatg agcagaaagt aatggaatca gggtctttca ggattgctat
    24541 atggaaaggg gtatggatgc ttattgagaa aaaaccatta actggatatg gtattgaatc
    24601 acttaaacat gcaatatttg cagatgatca ggtaagaatg tttctaagag agataagtcc
    24661 gcaaatagtg aatacagaca aagctcataa tgagttttta aacatttgtg tttcaagcgg
    24721 gatacctgca ggaattgctt atcttacaac cgcaataatt gttataacca aaggctttat
    24781 gaatatgaaa aaaaatcctt tcttgttacc attaacatgc tcagtaacag catattttat
    24841 acaaggcatg tttaatataa gcaataatgg gatagctttc atattttaca ttttaatggg
    24901 agtaattatt gctctatcaa aatcaaattc aaataaaata aaattaaagg ataatactta
    24961 atggaaaaaa tcagattgca gaagtacctt gcaaatgcag gagtagcttc aagaagaaaa
    25021 gcagaagagt taattgctaa aggtgtcgta tcggtaaatg gaaagatagt gaacgaagtc
    25081 gttttggttg atgataatga tgatataagg gtaaataata aaccgattaa aattcaggat
    25141 aagtttttat actatgcatt taataaaccc gaaatggtaa taacctcaat gtcagaccag
    25201 tttggaagaa aaacggtttc ggactttttt aataaaatag aagaaagagt atatcctgtc
    25261 ggacgtctgg attatgattc gtcaggattg atattcatga caaacgacgg ggattttgca
    25321 aattatctta tgcatccaaa aaatcaagtt actaaaacat attctgtcta cgctaaaggt
    25381 tctataacta gtgatattaa aaaaacacta gaagaaggtg tagatattgg agattatgtt
    25441 acagacaaag cagaagtttt tgatattgaa aataacgaag gaagatgtac agcttttatt
    25501 tccataaaag aaggtaaaaa cagacagata cgaaagatgt ttgaggttgt tggcattaaa
    25561 gtatatatgc tgcaaagagt ttcaatagga cagatatctt taggggattt aaaaaaaggc
    25621 cgttacagat ctctaaccgc aaaagagctg gaaacattag gttattgtaa aaagtaaata
    25681 atgatactaa ttactacaat gttatgaatc attaatcatt ttaattatat agttataatt
    25741 attatgatac ttttccttat tcctaaaaaa cgattgaatg aatctatcac aaatgatata
    25801 atgtattgca atgggcatta aaaagattac gaggtaacta catgagtatg atagaaaaag
    25861 taaatgaact aaaaaacaaa aagcaggaag catcgctcgg aggcggacag cttcgaatag
    25921 aagctcagaa ggcaaagggt aagttaactg cgattgaaag aatccacaaa ctttttgatg
    25981 aaggatcttt tgtcgagact gattctttta cacaatcacg aagtgtggat ttcgatatgg
    26041 cttccaaaag aaaagcaggt gatggcgtta tttgcggtta tggcagaata aatggaaggc
    26101 ctgtttttgt cgcatcacaa gattatactg tattgcatgg atctgtaggt gaaatgcatg
    26161 cgaagaaaat aacaaagaca attgatatgg ccattaagac aggtgtgcct atggtttatc
    26221 tgtttgattc agatggtgca agaattgaag agggtactga tgtacttgcg tcaattggcg
    26281 aatttatggc aaaacagaca atggctagcg gagtaattcc tcagatatca gctatattag
    26341 gaaattgtgc aggtatatcc tcattagctc cgactctatc agattttgta ttaatgactg
    26401 aaaagactag ttatatgtat atgaatggcc cagcagtaat tgaaagttca acaggcgtaa
    26461 atcctggcaa ggatgatatc ggttcagctt taactcatac aagtttaagt ggtaatgctc
    26521 acatatatta tgaaaacgaa gataaatgca tagaaggtat taaagatatc ttatcatatc
    26581 ttcctgacaa taacttatca gaggcggtaa aagttgaatg taatgatgat ccaggcagag
    26641 cttctgatga tatatatgaa gtaataagtg attcatatga tatgaaggat gttataacaa
    26701 aggtaacaga taactccagt tttatggaga tacaacctga attcggtcag ggtatcgtaa
    26761 tcggatttgc cagaatgaat ggtgattctg ttgggattgt tgcaaacaac ccagctgtta
    26821 atcaaggtgc tcttgatatg gattctgctg acaaagcagc tagattcatt aggttactag
    26881 acagtttcaa tatacctata ctttccttta ctcatacatc cggatatata gcagatgcac
    26941 agcaggaaaa gaaaggtctc attaggcatg tagcaaaaat gctttatgct tttgctgaag
    27001 catctgttcc taagattaat gtagtaactg gtaaagcaat aggtggtgca tttgtagcaa
    27061 tgaacagcaa atcaattgga gctgatgttg tatatgcata tcctactact gaaatttcaa
    27121 taatggaaag cgaagctgca gctaatgtta tattcagtaa gcagattgct caaagcgaag
    27181 atccaacaac ttttagaaaa gaaaaggttg atgaatatag acaaagatat ggtttggcat
    27241 acgaagcagc taacagaggc tatgttgatg atgtaattga tcctgcatat accagaacat
    27301 atgtaataag cgctttggaa atgcttaagt caaaaagaga agatcgccct aataaaaaac
    27361 atggcaacat gccgctttaa tacacggagg atatataaat gaaaaaattc atagcaaaag
    27421 ttaatggaag agaatatgta gtagaacttg aagaagttgg atcaacagaa acaaagacag
    27481 ttgaaactgt ccagactgta cagccagtac aacaaactgt tacacagcaa gaacctaaaa
    27541 agcaagaaga accagctcct aaaaaagctg ttacatctgg tggtgcagga tctgttaaag
    27601 ttgaagcacc aatgcctggt acaattttga acgttaaggt atctgtaggg cagtctgtaa
    27661 agaagaatga tgttcttgca gtacttgaag caatgaagat ggaaaatgat atagttgctg
    27721 ctcaagacgg agtcattgct tctgttaatg ttgcaaatgg tgattctgta gaatcaggtc
    27781 agcttctagt atcaatggac tagaaaggat aaaaaatgag cgtaaaaata actgaaacaa
    27841 ttttaaggga tgcccatcaa tcgttaatag cgactagaat gagtatagcc gatatgaaag
    27901 atgccctggt tatgcttgat aagataggtt atcattcact ggaatgctgg ggtggagcaa
    27961 catttgactc ctgcgtgaga tttttaaatg aagatccatg ggacagactt agatatatta
    28021 gaaagcatgt taagaataca aaacttcaaa tgcttttaag aggtcagaat cttttaggat
    28081 ataagcacta tgcagatgat gttgtagaat atttcgttca gaaatcaata tacaatggta
    28141 tagatataat taggatattt gatgctttaa atgatgcaag aaatcttgaa actgcaatga
    28201 gagcctgtaa aaaggaaggt ggacatgctc aaggaacgat atgttatacg acatctccag
    28261 tacacacctt cgaacaatat ataaaggatg ctaaaaatct tgtggatgca ggnnnnnnnn
    28321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    28381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngtagcatc attaaaggat actccatatg
    28441 atacaggtct ggatgtagca aagattaatg atatttcaaa attatttaaa gttataagag
    28501 aaaaatatat aaaatcagga cttttcaatc ctaaagttat gggagttgac attaatacgc
    28561 taatatacca ggttcctgga ggaatgcttt caaatcttgt taatcagatg aaggaagcaa
    28621 ataaagaaga tcaacttcag gatgttctta atgaagtacc taaagtaaga gcagattttg
    28681 gatatcctcc tttagtaaca cctacaagcc agatagtagg aacgcaggcg gttatgaatg
    28741 taatatcagg tgaaagatac aagatggtac ctaaagagtc aagagatctc gtagcaggta
    28801 aatacggaaa aacaccagtt gaaataagtc aagaaattat tgataagatt ttaaaaggtg
    28861 agaaacctat tacacacaga cccgcagatg atataccacc tgcattagaa gagactaaga
    28921 acgaaatgaa ggagtacttc gaacaggatg aagacgtctt aacatatgcg atgttcccaa
    28981 aagtcgcaaa agactttttt gaatatcgaa ggacattgaa actgaaactg gacaaagatc
    29041 ttaccgacat ggaggataaa gtccatccag tataaatgaa attaaaacgc ctttaggttg
    29101 cataaaagcc taagggtgtt ttctttgaaa ttgtttgctt gaaaaatgaa atatttaatt
    29161 agtaagtatg ctaaataagc attgtgaggt attaaatgag tatcaaggat aagcttaaga
    29221 acacaaaagg atttagtaag gggcagaaaa aaattgctga atatattctg aaaaattttg
    29281 acaaagcacc attttatact gctactaaac ttgctacaga agcggataca tcggaatcta
    29341 ctgtagtgag atttgcgatt gaacttgggt atgacggtta tccggaaatg caaagagata
    29401 tgaaggaagt ggcaagacaa actttgactt ctgcagaaag gcttatgcgc atatcagata
    29461 ctgttgaatt aaataacgtg cttatgtcag taatgaaatc agatatcatg tcaatcaagg
    29521 aaactattaa catgattgat aatgatgtat ttaacaagat agttgaaaaa atagctgatg
    29581 ctaaaagaat atacattttg ggaataagaa gttcattttc agtagctttg tacatgtcta
    29641 tatattttaa aatgattttt gatgatgttt atcttatagg catgccgggt gctgcagata
    29701 tttttgaaga gataatatca atttctgaag atgacgtatt tataggaata acatttccta
    29761 gatactcgaa aagaactata catgcaatgg atatagctaa gaacagcgga gcatatactg
    29821 ttgcaataac agatagcaag gatgctccta tttctgaagt tgctcacaat atactagttg
    29881 cgagaagtgg tcttactgga tttatagatt cactgactgc tccattttca gtagtgaatg
    29941 ctttaattgc agcatgcgga attaaaaaag ataaggaatt aatgacaaga cttcaaaata
    30001 tggaaagaat atgggatgag cattcaatat atacaataga aacgaagaaa ataaatgaaa
    30061 agtaaatatg atttgatagt aataggcgca ggagcaggcg gtatgatggc tgcaataacg
    30121 gcagctcaaa gaggattttc agttctcgtt atagaacaga agaagcagga aggaagcaag
    30181 atacttgtta caggaaaagg cagatgcaat atcacaaatg attgtgatgt tcaggatatt
    30241 ttgaaaaatg taatgacaaa tccatctttt ctttactcat ctttatatgc gtttcctcca
    30301 tcgagaataa ttgagttttt taatggaata ggagttcaga ctgttactga aagaggaaga
    30361 agagtctttc cagaatcaaa taaagctatt gatgttagaa atgcacttgt aaagcagatg
    30421 caaaaagcaa aagtagaagt attaaaaaat acaaaagctg ttgatgttat aaaaaacaaa
    30481 gatttattta cagtaaccat taaagatgta aatgatgtta aagatgatta taactgtacc
    30541 aatcttttac ttgcaacagg tggtatgaca tacagctcta caggaagcga tggctcagga
    30601 tatcagcttg cgaaaaaact aggacatgag ataactccat taaaaggcgc tttgagcggt
    30661 cttttaacaa aggaaaaatg ggtaaaagaa acgatgggac ttacattgaa aaatgtaaca
    30721 ctatctgtat ataaagatga ggcacttatt tataaagagt tgggcgaact tctgtttact
    30781 cattttggta tatcaggtcc tattgttctg acagccagca gataccttct ggacactgac
    30841 tataaaaaca caaaagcagt tattgattta aaacctgctc ttgatgaaga gactttatat
    30901 aaaagaataa ctagtgactt tgagaaaaat tcatcaaaaa tctttattaa cagtctagat
    30961 gcattattac caaaatcact gattccaata atagtcagat taagtggaat tgaaagtgat
    31021 aaaattgtca atcagatatc aaaagatgaa aagaaaaatc ttactcatct tttaaagaat
    31081 cttaccttaa cgataaaaag cggtatggat atggaggatg ctatagttac aaaaggcgga
    31141 ataaatgtta gtcagattaa tccttcaaca atggaatcta aaattacaaa acatgttttc
    31201 tttgcaggag agataattga tgtagatgcc ttaacaggag ggtataacct tacaatagct
    31261 ttcagtacag gatatcttgc aggacaaagc atttattaat ataccaaaaa gcttattatt
    31321 actaattaaa ccatttatta ataatgattt tattgtaaag ttgttgtaat atatatataa
    31381 tagacaggca aaaggaaaaa gattaatgga ttacgacaca ataggcagta aggatacttc
    31441 aaaagcggca atcaaactat ctattacttc tacacgggaa gaggaatatt ctctcataag
    31501 agattatgca gaaaaaggca taaaatgtgt agcagtagat ttcggaggcg attttatcgg
    31561 ctctgtttca aaaataatag aaagagcagt agttgcatca aaaagacaga atgttatagc
    31621 tgatgttcac catgaacaag gtgctgttgt tggagctact agagaagcat taggccagat
    31681 aatgcctaaa gctataggtt gtaatgtagg tggtaaaatt ggaatagcaa gacataagga
    31741 tcatataact gtagctgtat ttacaggtat aggtttttta aatttagatg aaattgggat
    31801 tggattaggt cataggagtt tatgagattt tcagctatag caattgatgg ccctgctgga
    31861 agcgggaaga gtctgatggc aaaaatgctt tcagaaaaaa aaggatatac atatcttgat
    31921 acaggtgcaa tgtatcgtac agtagcactt aaggcgataa gaaacggtat agatactctt
    31981 gatggggata aacttagtaa attaattaag aacattgaca tagaattggt tattaccgat
    32041 aacaaacaac acatgtatct tgataaggaa tgtgttgatg attttattag aacaaaccag
    32101 ataagcaaag gtgcatctga tgtatcaaaa tttccatctg taagaatgtt tttggttgat
    32161 atgcaacgta atgtagcaaa aaaacataat gttgtaatgg aaggcaggga tataggaact
    32221 tatgtgcttc cagatgctga atttaagttt tatttaactg caagtaatga aaaaagagca
    32281 gaaagaagat atcagcagat ggtacaagat ggaaaagaaa ctacatatga gcaggttctt
    32341 gaagatataa tatacaggga taatcaggac tcaaaaagag atttcgctcc tttgaaaaaa
    32401 gctgatgatg ctatattgat agatactaca aatatgggaa taaatgaggt atttgatact
    32461 ttgttaaggt acataaatgg aaaatgaatg ctttagaaaa acatgcagag ctattgtaag
    32521 aggaataata aaaatattct atagggtaga atatataggc gaagcttatc ctgatgaagg
    32581 ggcgtatata atatgtccaa atcatttcag tatacttgat atagtatttg ttcttgtaca
    32641 tgcaagaaga cggtgggtat atttcatggc aaaaagcgaa ctatataaac acaagttttc
    32701 agcttggttt ctaaataaaa tgggagcttt tcctatcaaa agaggacaaa gtgatgttcg
    32761 aagtattaaa aaagctgtta cattgcttaa ggaaggaaaa cttgtctgca tatttccaca
    32821 aggtactaga cagaaaacaa ataatccttt accaccaaga agaggtgttg caatggttgc
    32881 agctctgaca ggagcgaaag taattcctgt gtttatagaa aacaaagcta aagtatataa
    32941 aaaaacaagt gtatatattg gggagcctat tgacacagga ctggacagaa aatcaccaaa
    33001 ggaagaaatt tcacaaaaga gccaagaact tatggatata atatacaatt taccggaggt
    33061 atacagaaat aagtaaccga gtagtaattt caaaaaatgc cggattttgt tttggagttt
    33121 ccagagcagt aaaaatggca gaggaaatag tacaaaagga agatatcaaa tattcatatg
    33181 gtaatgtaat tcataatcag caggttataa atgaattaag ggaacaaggg ttaataaccg
    33241 taaatgatat ttgtgaaatt ccagatggtt cagctgtgct tgtgagagct catggagtat
    33301 cttcaacttt ctataatgca gcaaaagaaa aaaaacttaa actttatgat gctacatgtc
    33361 cgtatgttgg taatattcat aaaagagtac aaatgatgta tgaagaaggt tataagatta
    33421 ttattgtagg gtcaagcatg catcctgaag ttataggaat aaatggctac tgtaataact
    33481 cagcaatcat cctagaagat gaatcagagt tggaaaaaat agaaccagat gacaagaata
    33541 aatactgttg tgttgctcaa acaacattaa taaaagaaaa atatgaaaaa attacatcag
    33601 aattgaaaaa aaagtttaca ttgcttgagg tgtttgatac aatatgcagt gctacgtctt
    33661 tacgtcaatc tcaagcagta aaattggctt caaacgtaga cataatgata gttattggtg
    33721 atcctaccag ctctaacacg agaaagctat atgaagtgtg taaagagaaa tgtacacgaa
    33781 catatttagt ttcaagtgca atgcaggctg aggatatcga tataaattac aaagaaatgg
    33841 ttggcattac ggcaggggct agtacaccgg accgaattgt cagggaggta aaggaaacaa
    33901 tggaagataa tgcaaaaaag ggaacgcaaa acacatttga ggaaatgttg gaagattccc
    33961 ttgtgacgtt aagaaacggg caggtagtaa ccggtaaggt tattggtgtc aatgacaaag
    34021 aagtgtatct tgatataggc tttaaaattg atggcacgat atctgtagaa gaattccctg
    34081 taggcaaaga tggaagacag gttgccgttg gagatacagt ggaagcaatt gtaacaagag
    34141 taagtgacag agatggtatt gtctatctgt caaagaaaaa aattgatgaa aaaagaaagt
    34201 atgaagatat aaaagatgca tttacaactg gtgatgcaat taaaggaact gttaaagaag
    34261 ctacctcagg cggacttata ctcgacataa acgggttaga tgcttttatg cctatttcgc
    34321 atataagtac tcagtttcag agaaaccttc aaaaattcat tggcagaaaa ctcaaggtaa
    34381 gagttattga gtgcgatcgc agaaagagaa agcttatcgt atctagaaaa gttcttatcg
    34441 aagaggaaaa tgaaagaatg gagaaagaag tatggagtaa acttgttgtt ggtgatgttg
    34501 taaaaggcac agttaaaagt ttttctaatt tcggcgcatt tattgattta ggcggaattg
    34561 acggacttgc tcatatttct gaactttcat ggggtaagat aaaccatcct gaagatgttc
    34621 ttgaattggg tcaggaagtt gatgtaatta taaaagatat agataaagat aaaagaaaag
    34681 tatcgcttgc ctatagaaaa gaagaggatg atccttggta catggcagaa gaaaaattcg
    34741 ctcctaacac aatcattaca ggaaaagtag ttagaattct tccttttggt gtttttgtgg
    34801 aaatagcaaa agatattgat gcacttgtac atatttctca gatttctaat aagcgtctca
    34861 agagtgcatc tgatgtactt gaagaaggtc agatggttga tgttgcaatt attgatgtag
    34921 atcttgaaaa caaaaagata aatgcaagca ttaaagcggt tcagcctcta gatcctctac
    34981 ctactcaaga agagatagac agagaaaatc aaagagaaga gagaagacag caaagtataa
    35041 aagaacgtac cgagaagttt actcgcagag ttgaaaatgc tgaaacagct ccaactagag
    35101 atcatgaacc aaggcaatca agaaaacagc gtgaagagtt gcctgtaagc catgaagaag
    35161 tacttagcaa caaaataggt tctttattgg cggatttcaa gtttgatgaa gaaactgctt
    35221 ctgaagctcc aaagaaagaa aaaaaataat tattaaacag ataccttact gatgcaaaag
    35281 agaaatcttt tgcattttgt attgtataat aaataagagg tatgaattat ggaaacaatt
    35341 aatgatatgt caaaagacac tcttttaaaa attcttaaaa gcagggaagt gcgtattaaa
    35401 gaacttgaaa aagaaatctt gctttatcag aagataatta aagaattaat ggaaaaggcg
    35461 gaatccaatg ataaaaactg ataactttcc tagtttcgac aaatatatcg aaaatttaaa
    35521 aagtaaaaaa gttgcagttg taggtttagg aataggtaat attgatttat ttaggttttt
    35581 agctgaaaat agtgtaaatg ttaccggatt tgataaatct aatgattttg atcagttaaa
    35641 aagcgactta accagttata ctaatgcatc atttgtcttt ggttctgatt atctagataa
    35701 acttattggt tatgatgtaa tatttaaaac acagtcaatg agaagagacc ttcctcaatt
    35761 taaaaaagag acagataatg gcgcgattct tacaacagaa atgtgggagt tcataaaata
    35821 ctgcaaagct aaaataattg ctgtaacggg aagttcagga aaaactacta caaccactct
    35881 tattggcgag ttgttaaaaa ggcaaggata taaggtatgg gttggaggaa atatcggaac
    35941 tcctctgttt cataagttaa atgatatcaa taatgatgat tatgttgtac ttgagcttgc
    36001 ttcctgtcaa ttgcagatgt tcggaaacaa aagtccaaac atatctgttg ttacaaatat
    36061 aactcctaat catctggatt tccatgatag ttatgatgaa tatatctaca gtaaaagtat
    36121 aatatttaaa tatcagacat ctgacgatat gctaataatc aacgatgata atgatataac
    36181 taatagtttt aaaagccagg ctaatggcaa ggtatatgct ttttcgagac aaaagacttt
    36241 tgataacgaa gaaggagcat acattgaaga taattcaatt ttcgtcaaat tagggccatc
    36301 cagagataaa gtactggata tatcagatat tttagtacct ggaaaacaca atatagaaaa
    36361 ttttatggca gccattttag caacatatga tcttgttgat aagaaaacaa taagtaacat
    36421 agcaaaacac tttaatggag tggaacacag agtggaatat gtaagaacgg taaatggagt
    36481 taaattttac aatgattcca ttgggactac tccatctaga actgtagcaa gtttgcagtc
    36541 atttgaccag aaggttattc ttataacagg cggttatgat aagcatattc cttatgatat
    36601 aatggggcca ttgataaatg agaaagttaa aggtcttatc ctcatgggtc agacaaagga
    36661 caagataaaa caggcataca atgattattc ctgtacagta cctttaactg aagctgatac
    36721 attggaggat gctgtagcaa aagcatatca aatggcacaa gaagacgaca tagttataat
    36781 gtctccagca agcgcaagtt ttgacatgtt taagaatttt gaggtgaagg gaaatttgtt
    36841 taaaaaaata gttaactctc tataaacttt gaaagcaggt ttaaaatgaa gaagtatatt
    36901 attgcactag accagggaac taccagcagc agagcagttg cttttgacaa agaaggtaga
    36961 aatatcggat ctattaacaa agaattcaca caattctttc caagtccagg ttgggttgaa
    37021 catgatccat atgacatact tcaaagtcag atgggagcac tattcgaatt actaaagcaa
    37081 tattcaataa aaggtgatga aattgcagca ataggaataa ctaatcagcg tgaaactgtt
    37141 gttgtgtggg ataaattgac aggaaagcct gtgtataatg caatcgtttg gcaatgcaga
    37201 agaacagctg atatatgtga aaagcttaaa gataaaggat atgaaaagct tataaaatct
    37261 aaaacaggat tagttcttga tgcatatttt tctggaacga aaatcaagtg gatacttgac
    37321 aatgttgaag gagcaaaagg taaaaattta ctctgcggaa caattgatac ttggcttgta
    37381 tataatctta caaaggaaaa ggcacatgta actgatatgt caaatgcatc tagaactatg
    37441 ctatttaaca ttaatacact tgaatgggat gatgagatac ttgatatttt tgaaataaag
    37501 aaagaaatgc ttccaaaagt agtaccatct tcacatattg tcggtcatat ggaaatagag
    37561 ggtgtaaatg tgcctatagc aggtatagca ggcgatcagc aggcggcatt attcggtcag
    37621 acatgttatg ataaaggtga tgtaaaaaat acatatggta caggctgttt tatcttaatg
    37681 aatactgctg aaaaaccagt attttcagat aaaggtttaa taactacaat tgcttggaat
    37741 tgtgaagagc agacttgtta tgctcttgaa ggcagtatat tcaatgcggg ttcttcagtt
    37801 caatggctta gagatcagat aggacttgtt accacagctg cacaaacaga agatatggca
    37861 ttagctgtcg atgatactaa tgatgtctat ttcgttcctg cttttacagg tttgggaaca
    37921 ccatactggg atatgtatgc aaggggatta ctgataggat taacaagagg aacaaacaaa
    37981 aaccacattg tcagagctgt tttggaatca atggcatatc agacaatgga tgttatagac
    38041 tgtatgcaac aggaatcagg tgtgaaactt aatacattga gggtagatgg tggggcaagt
    38101 gcaaataatt tcttaatgca atttcaatca gatatgcttg attgtgaagt agtaagacct
    38161 gcaatacaag agaccacagc attaggagca gcttttttag caggacttgc agtacgtttt
    38221 tggaaagata caaaggaact aaaaagagta attaaacagg acagaatatt ttctccgaag
    38281 gactattttg acagggaata cagaaaaaaa agatggcatg atgccatcga aaggtcaatg
    38341 aactggagca gataatcttt agtaatagaa atcgtgattt ccgatacttc cgtataatgt
    38401 acgacaatct gctatccacg tgcttttagc aagtcttgga ttgaaaaaga atagcgacca
    38461 tgccacattg ttatatccat ttaaagcagc ttttgcagca agaacagctt catcagaagg
    38521 ttcattgtat acggtccctg cagcaacagg tgtaaactga gtaccaccgg ctttgtcaaa
    38581 aataacacca tatatcgtat caggaaaatc ttcatgctta acgcggttaa gaactacatt
    38641 cgcaacagct accattcctt cataagtttc atgtccggat tctgcataaa ctatacggga
    38701 aagccagtaa acatcttcaa atgtgtatga ctcattaaag aaaaaggttc tttcagtcaa
    38761 taatattgaa tcagaaacct catcatacat agttttaatg cctattgcat cacacattac
    38821 cttaagtgaa gcatagattt tatcattgta tataacactt attgcaggcg attcaagcat
    38881 gatcccattt tttaacatat aactggttcc ggcatatatt atgtattcat tatacccatc
    38941 tgttatatat acactttttg tttcatttct ccattcatag ctgtatgaac caaaataatt
    39001 aagaaattct gtcactgata ctacaagata cccgtttata atgagtttat cattataaaa
    39061 attcacattt ttgtagttat atattactgg agaatttgta atataatact ttgtatctgt
    39121 cgggaaaatt tggtttgtag aagcgctaac gtcaattgac attagcggtg aaagcagaat
    39181 tatgatggct agcagcgaag ttattattct tctaagtctc aaacattctt tcattattgt
    39241 gacataatac acgatatgca atcataatgc aagacttaat tttcaaattg tgaaaagaat
    39301 gtgaacaaat cttttaagat gatttcatat caaatacacg aatagcgatt taataagctc
    39361 tataatcaaa attcatagga gagtagggta agagtaaaaa aaaaattaaa aaaatatatt
    39421 gacagcatta actagacatg gtacattatg catgtacaaa ggcaaaactg tagaaatgca
    39481 gtgacgcaaa actatggagc ttcgttcaaa agatcaagcc cgccaggttg ctacattgga
    39541 cttaaatgag ttcgaatgta gcttttttat tagtaaaaaa gctgcgatta tgaacaaatg
    39601 gaggtcaagt attttaggtt tgatgggtga atacaatggc agcaacagtg aaaggcataa
    39661 gtaaatatga gctccgcagt atggaaatat actacctacc tgttaaagac agacaactcg
    39721 gtattactca ttcatacatt tcacaattaa gaataaacga tccaaacatg ggtgttttac
    39781 ttgataacac ttttatgcca gttgctgaaa gaactgttat ttgtgaaaaa attacatcat
    39841 ggttaataaa caaccttttt tctgatatta ataaattcag aaacagagaa gttggagccg
    39901 aatggttcag tgtttatata ccagtcagag tgttggtaaa agacgatttt ttagaagaac
    39961 ttaaaaatac tgctaataca atgaacatta atcttaaaga tttatgttta tgtttcaata
    40021 acaagatatt gtatgaggat catacagtta ttactaatat tttcaaacaa atgaaagaaa
    40081 tgggaatcaa aaccatgata atggatttcg gtgatgattt ctgtcccata acaagaataa
    40141 gcagcattat gcctgattat gttttgttaa gccaaagtgt aacagattct ttgttatctt
    40201 ctaaagaaga aaagaagatt acagcaattt cactgtataa cttcttaaaa tcaattaatg
    40261 tagaagcaat tacctcctca atcaaggatg atgagatggc tgaaatatta cctgaatcat
    40321 ttaatttatg tacaggtaca tatgcaggca actatagaaa accaaggagt gtcagataaa
    40381 atgagaaagg atccttcagt tactaaaatc gcatataaag tacacaagac ctctctttta
    40441 ttcagagcct tgacaatcag ccttgttgca atagtaatac tgttgtcggt agcttatctt
    40501 gtttcagcat tttatgacag atacagtagt tttacagtat ctacttcata tagcggaagc
    40561 ggaagctcag gcaaaggagg aagtgattca caaggcagtt ctcttttaaa acttgcattg
    40621 tcacttagtg agacacctga ttttacagag cctacaacaa gattggatgc aaatgctata
    40681 gagaactgca cgaatatttc aggctacagc attcctgaaa atgtagacca gataaacggg
    40741 cagcataatg gagcaaacta cattgcttat acattctacc ttaagaatgc tggttcagaa
    40801 gcagttacgt atgaatatgc ggttaacatg cttaatgtta ctcagggtgt tgatgaggct
    40861 gttaggataa ggctttatgt tgatggagaa cagacaacat atgctaaaac aaaaactgat
    40921 ggatccggtc ctgaaaaaga tacagtagaa tttttaactg aatcggtaat tgtaagaaaa
    40981 agacgtgctt atttcaaacc ggaagacata acgaaattta ccgtagttat ctggattgag
    41041 ggagatgatc ctgaatgtac agatgacata ataggcggaa aatttaaagt tacaatggat
    41101 attaccattg tagaaggatc ttagaaaata ttgctttgca aaaagtatat ttaaatatta
    41161 taataataaa ggagttataa aaaaatgaaa acaatcaaag cgagacacaa a
//
LOCUS       DAOSXM010000013        35494 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2111
            NODE_785_length_35494_cov_12.517086, whole genome shotgun sequence.
ACCESSION   DAOSXM010000013 DAOSXM010000000
VERSION     DAOSXM010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864178
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 35494)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 35494)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:24:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,261
            CDSs (total)                      :: 2,229
            Genes (coding)                    :: 2,218
            CDSs (with protein)               :: 2,218
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 3 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35494
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_785_length_35494_cov_12.517086"
                     /isolate="AMR_MDS_2111"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(197..613)
                     /locus_tag="PLQ68_03330"
     CDS             complement(197..613)
                     /locus_tag="PLQ68_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003486120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HXK71716.1"
                     /translation="MFDLITRNKRNDYSVLDLMNDFFSTDVMTSTQGFKVDVSENDKE
                     YLLEAELPGFDKKDISVELKNNYLTISASRDCEKEEKEKNFIRRERCYGEFSRSFYAK
                     GVKQEDINAKYENGVLLVKLPKKEKEEAPSTTIEVK"
     gene            complement(799..1728)
                     /gene="dusB"
                     /locus_tag="PLQ68_03335"
     CDS             complement(799..1728)
                     /gene="dusB"
                     /locus_tag="PLQ68_03335"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015357860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HXK71717.1"
                     /translation="MIIQGKEIKGLFLAPIAGITDAPFRTICIEHGAVLTYSEMISAK
                     GLFYDDQKTKKLLSKTNPSEPFAVQIFGSEIEPMQYAAQYISSLNPVLIDINMGCPVS
                     KVVKNHEGSALMKDIKKAFKLTKAVVDAASVPISVKFRSGFEKHNLNYVEFGKAMESA
                     GASCITLHPRTRDMFYSGEADWTHIISLKEAVSIPVIANGDVFTHKDIEDIRKKTGCF
                     DVMIARGSLGNPWIFSNIIPTTEQRIKVFKHHMDLQIEYNGEKKGIMEMRKHGAWYFK
                     GLHNSAKLRDMIFKAETKQDVVNCIESVLEKEI"
     gene            complement(1770..2198)
                     /locus_tag="PLQ68_03340"
     CDS             complement(1770..2198)
                     /locus_tag="PLQ68_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005344068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HXK71718.1"
                     /translation="MKIDSIVNGIVLDHIKAGNSMRIYKYLNLEELKCSVAVIRNCRS
                     RKYGIKDVIKIDSIIDMNLDVLGYIDPNITVNIIRDGKISEKKHLELPVKLVNVIKCK
                     NPRCITSIEQEIDQVFNLTDRENRIYRCAYCETKSDKEEF"
     gene            complement(2210..3127)
                     /gene="pyrB"
                     /locus_tag="PLQ68_03345"
     CDS             complement(2210..3127)
                     /gene="pyrB"
                     /locus_tag="PLQ68_03345"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009527251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HXK71719.1"
                     /translation="MKHLMDITDLTTSQIDDLINTANDIIVNPEKYQDICKRKKLATL
                     FFEPSTRTRLSFEAAMLELGGSVIGFSTSDSSSSSKGESVTDTVQTVSFYADIIAMRH
                     PKEGAPLAASRRTTVPIINAGDGGHSHPTQTLADLLTIHREKGRLSNMTVGFCGDLKF
                     GRTVHSLINALSRYDGIEYVFISPKELVIPDYVKANILDKNNIKYIETNKMDEYMPSL
                     DILYMTRVQRERFFNEADYIRLKDSYILTIDKLAKAKKDLSIMHPLPRVNEISVKVDD
                     DPRACYFKQVQNGKYIRMALILMLLDILV"
     gene            complement(3138..3770)
                     /gene="pyrE"
                     /locus_tag="PLQ68_03350"
     CDS             complement(3138..3770)
                     /gene="pyrE"
                     /locus_tag="PLQ68_03350"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009597415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HXK71720.1"
                     /translation="MKRKIAEHLLNIQAVFLSPDKPFVWASGIKSPIYCDNRITLSDH
                     LVRADIEKGLAELIIKNYPQCEVIMGTSTAGIAHAAITAHILGLPMGYVRSSAKDHGR
                     GNRIEGRLLPGQKTVVIEDLISTAGSVIDTVEALREEEAQVLGIASIFTYGMAKGVQR
                     LKETNVQNISLTDFDTIIEVACEKGYIKQNDKNKLLTFRDNPSDEGWIEA"
     gene            complement(3767..4480)
                     /gene="pyrF"
                     /locus_tag="PLQ68_03355"
     CDS             complement(3767..4480)
                     /gene="pyrF"
                     /locus_tag="PLQ68_03355"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019130352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HXK71721.1"
                     /translation="MKKDVIIACDFPSKKDVMDFLNLFGQKKPFVKIGMELFYAEGPD
                     IVRDIKEKGHKIFLDLKLHDIPNTVERAMRSLSLLDVDMVNLHTSGTVKMMQAALKGL
                     TREDGTRPLLIGVTQLTSTNESELHEQLMINSSMDNTVMHYAKLAVTAGIDGVVCSPL
                     EAEKVKNTCGKSFVTVTPGIRFKHSRQDDQSRITTPYQASKIGCDFIVVGRPITQSDD
                     PVGVYRRCIEEFVYGKETQ"
     gene            complement(4480..5391)
                     /locus_tag="PLQ68_03360"
     CDS             complement(4480..5391)
                     /locus_tag="PLQ68_03360"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006525320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HXK71722.1"
                     /translation="MVNLKVNLSGLELSNPIIPASGCFGFGKEFIQYYDINILGSFCT
                     KGTTKEARFGNDTPRIAESSCGMLNSVGLQNPGILHVVRQVLKEISSYYSKPVIANIS
                     GFSIEEYEYCAKSLRGIDNIGLIEVNISCPNVHEGGMSFGTNADMAAKVTKAVVKVAD
                     KPVYIKLSPNVTDIVSIAKACEEAGAAGICAINTLLGMRIDIKNRKPVLANITGGLSG
                     PAVFPVALRMVYQIYKAVKIPIIGIGGISSTDDVIEMMMAGATAVQIGSMNLIDPCIC
                     KTIIEDLPERLNRLKVKDINEIIGGAH"
     gene            complement(5385..6110)
                     /locus_tag="PLQ68_03365"
     CDS             complement(5385..6110)
                     /locus_tag="PLQ68_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019130354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HXK71723.1"
                     /translation="MGIYTVNNIRKLTYDTYEMVLSGDTSWIRAPGQFVNIKIDGLFL
                     RRPLSVCDIADDKMTLIFKVVGQGTEILSCMKPLQQLDILCNLGNGYDTEVFTLKPLL
                     IGGGCGVPPLYYLAKTLIKKGKIPNVILGFNKKNDVFYEKEFADIGCKVYVCCVDGSY
                     GYKGFATDAISMIDYDYIFTCGPEPMLKAVYENSSKPGQFSFEERMGCGFGACMGCTC
                     KTKYGYKRICKDGPVLFKEEIIW"
     gene            complement(6092..7273)
                     /locus_tag="PLQ68_03370"
     CDS             complement(6092..7273)
                     /locus_tag="PLQ68_03370"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HXK71724.1"
                     /translation="MLLKNAFIYIDGSFKSSDLYIINETEWIIGEDLSSIKCDQIIDL
                     SKKYIVPGLVDVHVHLREPGFEYKETILTGSESAKAGGFTSICAMPNVTPSPDDIDNI
                     RLIQDIVDKDACINVYQYSCITEGRLGQNLVDVSKLKDFVIGFSDDGNPVSSDLIMKK
                     MIEQSAMHDVLIAAHCEDFSYEDPRMSEYMNIKRDIELLKNTDTSCRYHVCHVSSKKS
                     IELIRKAKKDGISVTCETAPHYLVFTKEDVISENFKMNPPLGDKEDKEALIQAVCDGT
                     IDIIATDHAPHTYEDKLAGAMGVIGMETSFSSMYTYLVKTGIIKLEKLIELMSVNPSC
                     IFRIPKYPDFTVLDLDAENTVKSDGFLSLGRNTPFEGMTLNGRVIKTICKGKIVWEYT
                     Q"
     gene            complement(7334..8680)
                     /locus_tag="PLQ68_03375"
     CDS             complement(7334..8680)
                     /locus_tag="PLQ68_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012056306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HXK71725.1"
                     /translation="MGKTVNERFTLKTAVLSFQHMFAMFGATVLVPILAGMSISVALL
                     SAGIGTIAFYFITKKKVPVFLGSSFAFLPALIASMAGAGDIGSATWNVAMGKTSVAIM
                     MAGLVYVIFSLLLKKIGVERIRKLFPPIVVGPVIIVIGMILAPKMFYNNIIGQSIWNN
                     IPAWKEWTAAGITALTILVVSAVAKPKSFFKVIPILMGFVVGYIYSLIIGLVDYSTVD
                     WSQIIIFQDLGKTFSFYKNLSFDLGVILSVVPIAIVTFMEHLGDISANSTVCNKDFMV
                     DPGINRTVMGDGVATFIAGALGGPPNTTYGENTAVLAITKNYNPRNIFYAAVMAVIFG
                     SITIFGTAVSTIPSAVIGGASIVLFGMISAAGLRALVDGKVDFSDTKNILVVSVILSV
                     GLGLGAMSLAGDVTGDTAYKLMIGAVEISPLAVATLIGIILNLVIPADKKDKAESK"
     gene            complement(8715..9251)
                     /gene="pyrR"
                     /locus_tag="PLQ68_03380"
     CDS             complement(8715..9251)
                     /gene="pyrR"
                     /locus_tag="PLQ68_03380"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013158312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HXK71726.1"
                     /translation="MEFKATLMDEDAVKRSLIRISHEILEDNKGPEGICLVGIKTRGI
                     PLAERIAKNIEHISGVKVPVGILDITLYRDDLSKCSEDPILNATDIQFKITGKNVILI
                     DDVIFTGRTARAAMDAIFSIGRAAKIKLAVLIDRGHRELPIRPDFVGKNVPSAKGEMI
                     AVKLLETDGITGVSLYRI"
     gene            complement(9470..9781)
                     /locus_tag="PLQ68_03385"
     CDS             complement(9470..9781)
                     /locus_tag="PLQ68_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016526344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjQ family protein"
                     /protein_id="HXK71727.1"
                     /translation="MKLLTIDYVPGKEIEDLGLVQAGMVQSKNIGKDIAASFKTLVGG
                     ELFGYTEMMNESRAMATKRIVEQAEKMGADAIIGIRYVSAAIVQGAAEVVAYGTAVKI
                     K"
     gene            complement(9787..10593)
                     /locus_tag="PLQ68_03390"
     CDS             complement(9787..10593)
                     /locus_tag="PLQ68_03390"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YhfC family glutamic-type intramembrane
                     protease"
                     /protein_id="HXK71728.1"
                     /translation="MVSASAITGIVFTLIITLIVPVVIWIVFAKKVKGISKAIIAGAL
                     GFIIPQLFIRIPVLQLLSTNPSVTQFSKDNKYFFLLILAFSAGLFETTGRLIVLKVLS
                     KNELSYYSSLGAGYGHGAAEAVGLIGLTYINNIIFAIMINTGSLPLGEQYESIKDTFL
                     TTSSSLFYAAGIERILTVIFHIGLTVFLAYMMTKKRTFAGFVLCIVLHTLLDFTVVSV
                     SMLTQNTWISLAVLLVFTVISAIYIIKFKNKFKIKEMPMDPAAKALEEGY"
     gene            complement(10703..11458)
                     /locus_tag="PLQ68_03395"
     CDS             complement(10703..11458)
                     /locus_tag="PLQ68_03395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK71729.1"
                     /translation="MNLRASYKYVMNDNKKALLGYSLIIIAVTVLLFIATISWSNDSN
                     TMQIGGMEMASMIFIFIAGLTAFKENFHMLTQNSVSRKGTFTATMLGFVTSSAMLSVI
                     SILVLLIAKLMSHFQSNIISESFFERIYIGHVAGMNDIVVFLESLLLYFVMLLMIVSI
                     GYLIGVIFYRLNKIGKVALAVGIPVFFTVILPVVDVLIFDGQITIGIIRFMDIIFGIS
                     AQNPYLAVVSCLVIFLITGLLSWFTMRKAPMKA"
     gene            complement(11455..12318)
                     /locus_tag="PLQ68_03400"
     CDS             complement(11455..12318)
                     /locus_tag="PLQ68_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012198216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXK71730.1"
                     /translation="MSGIEVKGLSKSYGSTQALRQVSVNFEKDKIYGLLGRNGAGKTT
                     LLNIITNRLFADSGEVLLDGKPIKENDDALSNIYMMSEKSLFPEKMRVKDVFKWTSKF
                     YPSFDMDYALKLASKFELDLKKKRDSLSTGYGSILKLITAMSVNVQYVFFDEPILGLD
                     ANYRDLFYRLLIEKYSEKPFTAVVSTHLIDEVSSIIEHVIMIDKGVVIKDEPLESILS
                     QGYTITGPANAVDLFTADKDVMSYDMLGGLKTAYVSGKAPVDIPENIEVTKMDLQKMF
                     IKMTNEKGVSK"
     gene            complement(12315..12683)
                     /locus_tag="PLQ68_03405"
     CDS             complement(12315..12683)
                     /locus_tag="PLQ68_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009263598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HXK71731.1"
                     /translation="MNIDFESDKPIFVQIAEWLEDAILSNAFEEESQIPSITEISVMC
                     KINPATALKGINMLVDNEIVYKKRGLGMFVKEGAVKKLMKERKNKFYESYIAVLLDEA
                     KKLNLNKEDIMEMIERGYEK"
     gene            complement(12881..12955)
                     /locus_tag="PLQ68_03410"
     tRNA            complement(12881..12955)
                     /locus_tag="PLQ68_03410"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12920..12922),aa:Arg,seq:ccg)
     gene            complement(12992..14227)
                     /locus_tag="PLQ68_03415"
     CDS             complement(12992..14227)
                     /locus_tag="PLQ68_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009004751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK71732.1"
                     /translation="MELTKKEISVLQEMAKQYMEYATLPIQKEKIRLWKALNRSKMER
                     PMVVIDQIPWNEMNNEHELDLFVEDPVFRRVEYYLKREVYKYKHYPVDMVLDPFIRVP
                     KAISNTGYGLKVEEESLYASGNVSSHVYTNQLIKEEDANKIKDMVITHDEEETKRRYE
                     TASEIFKGIAPVMMEGNMFHLGVWDHLSQFMGVENIYFDLVDEPEKLHVFMERITTSV
                     IKGIEQSNKLKIYDTNANTCHCSYIYTDELLPDSGMGKETITKNGWAFGLAQLFSSVS
                     PSVTEEFELPYIKRMAEYFGMIYYGCCDRLDDRLDLVLTIPHLKKVSCSPWSVREAFA
                     EKIGPKVIMSNKPTPALVATASLDEDEIRRDLQRTVDAARKNNVNLEMILKDISTVKC
                     EPERLTRWANIAMDVVNNY"
     gene            complement(14455..16242)
                     /locus_tag="PLQ68_03420"
     CDS             complement(14455..16242)
                     /locus_tag="PLQ68_03420"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019241225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008736 - L-fucose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019317 - fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HXK71733.1"
                     /translation="MNSNVERLKGTLPKVGIRPTIDGRRRGVREELEPVVMGMAYAAA
                     ELISQNLKYPNGMPVECVVADTCIGNVAEAAACAEKFAREGVGLTLTVTRCWCYGSET
                     MDMDPHMPKAVWGFNGTERPGAVYLAAVLAAHSQKGLPAFGIYGKDVQDANDNEIPDD
                     VRDKILNFVRAGLAVSYMKGKSYVSLGSVSMGIAGSIADPNFYQKYLGIRNEYIDMSE
                     FIRRMNEGIYDKEEFKTALEWTKKNCKEGDDFNKTPHTREQKDKEWETCVKMAIITKD
                     IMTGSDYLNKNGFEEEGCGRNAIMAGFQGQRQWTDFMPNGDFMEAILNSSFDWTGIRQ
                     PYIVATENDGLNGTAMLFGHLLTSGTAQIFADVRTYWSPEAVKRVTGHTLKGYAKNGI
                     IHLINSGAAALDGTGRCEEDGKPVMKPFYEISEEEAQACLDNTTWGPANLGYFRGGGF
                     SSTFLTKGGMPVTMTRMNIVDGLGPVLQIAEGWTVDIDPKAHEMLNDRTDPTWPTTYF
                     APRLTGEGAFTSVYEVMAKWGANHGVLTYGHVGDRFITLCSMLRIPVNMHNIENDRIY
                     RPSAWSSFGTNDLEGADYRACNNFGPLYK"
     gene            complement(16293..18167)
                     /locus_tag="PLQ68_03425"
     CDS             complement(16293..18167)
                     /locus_tag="PLQ68_03425"
                     /inference="COORDINATES: protein motif:HMM:NF021489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HXK71734.1"
                     /translation="MKKIVIYRSIALIALSVLLICAFSHVYAYDVNGYHISSYDTIID
                     VQENNVLYITENITAVFTADGKHGIIRTIPTVFENRKVHIDNIYVDTTYSVESSREYI
                     AIRIGDADRYVKGEVKYTISYYYDYGDDGNENYDKIYHNILGTDWDCDVNNVTFAVNM
                     PKSFDKDNISITYGDRYSTSKYVDYTVTGNTIRGEVSDLYSYQGVTLALELPEGYFVN
                     ERPIDGIDIPVIAITAPIAVLVLICCVLQWYKKGKDDQLFAAPEFYPVKGQSPAEAGY
                     IIDGVIDNKDITSLIFYWADKGYLEITDKKWSYEFTKLQEPKFDNEYEQYMFDKFFEY
                     GVNNTVSTNDLKDHFYTAIPVIKQKIKYVFRKDRELVSKDSLKNRAKSALITVIPFIV
                     FLIISWIEFKEAAIMIPLTIFYGMFYGMILGLYVSAENKWNITGNFKKFLIAVLVTIL
                     LVISASIADLMLASIYPYYDVTTYSIAYFIIAFITAIALAYSMAMKKRSEYGQKMMEH
                     YLGFKDFIATAEMDRIKMLIDENPKYFYNVLPYAIVLGLEKKWGKKFESIVVEPPQWY
                     HGYNMRTFSTVYLLSRMNSCLNQTTASMAKAPSTSSGGGFSSGGFSGGGGGGGGGSSW
                     "
     gene            complement(18173..18733)
                     /locus_tag="PLQ68_03430"
     CDS             complement(18173..18733)
                     /locus_tag="PLQ68_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006057624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HXK71735.1"
                     /translation="MGWTILGYVVLGVILLAAVYFIAAYNSFISLRNKFEEANATVDV
                     YCKKRYDLIPNLVETVKGYAKHEEQTFMKVVEARANAMQSKTSAESQANETALEGALK
                     SLFAIAENYPDLKANQGFLDLQSQLSGIESEIASARKYYNAIVKNFNTKTQTIPSSIV
                     ASICKFEKQPYYEVMEEERQNVKVSF"
     gene            18929..20548
                     /locus_tag="PLQ68_03435"
     CDS             18929..20548
                     /locus_tag="PLQ68_03435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK71736.1"
                     /translation="MKNYFTLISNLADKQIHPSVNEATNQKVIIKKNETNCPSSYGIK
                     KAVIFLSACFNSFSAYYKSPKLMKQTLESILAATKYVTKGSYEPVSFCISVIQASNIF
                     RLAKTYAKILSSCDKHLGNEIQNAALDYMEKSKDVLMNINTDNLYLYSALCRLKKDLK
                     DEIITNAADNIKQSVTNLSKLDNVSICVLCDIYRETKDKSYLKAAQNNMENVLKLLEP
                     DDTVISIEDEKNNLKNRKYPLCIQDYLGPLLYLSLVSKNEQLDKALFYANKALVVDEN
                     PDIFESENLLFIPSLVLLNKGLLDIEISDITRVEALSSFSGYVSDFGIYRHGTGTQIS
                     QTIYKDQDLFYSIQSGALKLRLRLNATFFGPKGRFKSTDIEKTEKGIRLHYQRQWGYW
                     LPLEDKSVPPIIGSDINKIDRKMIRLQDFDVEVYIDLLKDGADITMTLSKTDEIPCKL
                     DLIFDKFGVFDSDSASINTIGNDYIQLQNGYFSYSSGSDYITAGPAFNGSSIYSETLR
                     GSNPPIDNTFTVYFTDMTPTTRTISIRGGKA"
     gene            20545..22134
                     /locus_tag="PLQ68_03440"
     CDS             20545..22134
                     /locus_tag="PLQ68_03440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK71737.1"
                     /translation="MKELIKERIHRSDDIIENLIEGQCLDKNSFYYGAVIDHTTGYAE
                     PSRGISIAGYFILKYCLPDSKFYNYDLFLERAIIAIKYSLKRLHADNTFDLTCTNPHD
                     STSIAFSVRIVAPALRLLKRHMEKKDDVKKIEIDVHNALVDFLTKSVDGMVGNGFHTP
                     NHRWVVASALALDMNILGMPELMDEINKYLDEGIDCDECGEYAERSISIYNLTNNESL
                     IILAHELNRPDLLEHVKRNLYMTTKYFEPDGTLFTLNSTRQDFGTKTYPFALFESYMV
                     MAHLDNNSDFAYMAKHIFDMSSNNPYDISDQGYYSFAGRFVQHMEYTDHYSGNVEIKP
                     FDFSHYDSFFPNSDIARIRDDDTSMTILAKNVMTMKYQYKFLSIHMMCMADGYVECEK
                     IEKTDKGYRLVSHDKKTDITLDFEFKKDGAEIKVDAKSNQPYPCTIALMFDKEGWLSS
                     DDYSLQARMGNNIFIKNKKFKYSHSVKFYPYSLFIETDFEDEGFADLAISKVMPTDKN
                     AFTVLFSAEAPFNKTIVIKKS"
     gene            complement(22136..23392)
                     /locus_tag="PLQ68_03445"
     CDS             complement(22136..23392)
                     /locus_tag="PLQ68_03445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK71738.1"
                     /translation="MNYKIEKWEREYLRDYAKKQLEISKLPVMQERIKRWYDHNENKK
                     GKPMIIIELASFRDDVRIPLKTQSDFARNIESAIQNNIQVHELLDDDEVCPDFLTFGW
                     DIEYKEFGFDIPVVKSKDSFGREIGFEYQYPIKDLETDFEILKPFTYTVNKENSFNRI
                     EAARDILGDILKVEMESGFHRWGNMISLKAVQLMSMERWMIAMIETPEMSHKLMDYIT
                     SNMLSYQHWLEENGLLTMNNGNYYAGAGSRGFTHELKMPEDGKVRLKDMWVNVNSQES
                     TGISPSMYEEFVYPYIERIAKEYGMVYYGCCEAVHPIWDVCLKNLSNMRKVSISPWCD
                     EQFMGERLRGTNIIYSRKPSPHFLGVGYDFDDDEYSKYMLNTLKAAKGCNLEIIFRDV
                     YTLSGNPAKAKRAVRLLRELIDKYWE"
     gene            complement(23554..24708)
                     /locus_tag="PLQ68_03450"
     CDS             complement(23554..24708)
                     /locus_tag="PLQ68_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009251991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HXK71739.1"
                     /translation="MSQDILNKFKQIHDEKKKESTDRVLKIGIIGTGWIAEPHVTAYQ
                     KCDDAKIVACADLIDGKAEAFCKKMGLEDVRLYKSHKELLDNEELDAVSICTYNCTHA
                     ECAIYALEKGVHVLLEKPMCVTLDEAVAIMKAEKASNKILSIGFQPRFDENMKMIKKI
                     VQSGELGDVYYIQTGGGRRRGIPTPFGTSFIAKDTGGIGALGDIGCYSLDMVLNAIGY
                     PKPLTVSGYKSDFFGKRPEYYPNHPEYASAFGVDDFAAAFVRLEGGIVLDFRISWAMN
                     MDTAGDTLILGTKAGLRIPSTECWNGSVGGAMKLYHEVCGKQVETVIPIIEKKGNLFD
                     MKIRSFLDAIIEDGESPIPSSQILYNQAIIDGINKSAELGREIEINIPEV"
     gene            complement(24722..25558)
                     /locus_tag="PLQ68_03455"
     CDS             complement(24722..25558)
                     /locus_tag="PLQ68_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009254444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HXK71740.1"
                     /translation="MSLPVAIQLYSVRNDLDSDFQNVIKKIKEYGYDGVEFASLHNQE
                     PEYVKSLLDETGLIPVSAHVALDEMLESTDEVFKTYSFIGCKYIAVPYLPEERRPGKP
                     LFDKALKQIEELGLKAKDYGLQLLYHNHDFEFVKVNGKFGLDVIYDTISKDLLKTEID
                     TCWVKVAGVDPSEYIEKYTNRSPVVHLKDFYKQDMAKDVKMYGLIGVNSDDKESKKDN
                     QEAFSFRPLGMGMQDIPAILEASVKAGAQWMVVEQDKPQKGRTALECAKLSIKYLNNL
                     IW"
     gene            complement(25678..26193)
                     /locus_tag="PLQ68_03460"
     CDS             complement(25678..26193)
                     /locus_tag="PLQ68_03460"
                     /inference="COORDINATES: protein motif:HMM:NF025879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HXK71741.1"
                     /translation="MAQIVKVYRQTIPAFRFIGKKYSNSDRQNGTFGAKWSEWFQNGW
                     FDEVEALSTIKNEYEDSDSYVGLMRCKHNDAFEYWIGIFMPKDTKVKEGFSYVDFPES
                     NLGVCWIYGKENELYMHEDECGKRLEEEGYEIIPDEKGAYWFFERYGCPRFTTPDDLG
                     NVILDICYFVK"
     gene            complement(26220..26735)
                     /locus_tag="PLQ68_03465"
     CDS             complement(26220..26735)
                     /locus_tag="PLQ68_03465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK71742.1"
                     /translation="MAEIIKTYIQQVPALRFIGKKYTEEDKVNGSFHAKWSEWFNRGW
                     FDILEDNLIPHKEYEDGCAYVGLVCHKNTGTPEYWIGMLVSQESPVPKGFDYIDYKHN
                     KLGVCVIFGNKDELYNDEDTYIEKLRETGFDVVECEEGPCCFFQRYGCPCFMVPDEDG
                     NIVFDLCFYVK"
     gene            26916..27626
                     /locus_tag="PLQ68_03470"
     CDS             26916..27626
                     /locus_tag="PLQ68_03470"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HXK71743.1"
                     /translation="MRLMTYNICSGMSYGEDRRRDLNQCAEVIKKYNPDILGLNEVHY
                     NIGFSGFAKQAEELNKILKYEYMYYGKAGAIKEGYQGNALFSRFPIKTAKTVLVDQPS
                     ICDEDTYYEQRSFIDSIIEAKNDLRVIVTHFGLAKGERKNELNLLLPIFRNHTDKLVF
                     MGDLNVEPDDVQLKIISSALKDTADITKDNLYTWPSDIPVKKIDYIFVSQDIKTDSVN
                     VPTTMASDHLPYIADVSF"
     gene            complement(27623..28423)
                     /locus_tag="PLQ68_03475"
     CDS             complement(27623..28423)
                     /locus_tag="PLQ68_03475"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXK71744.1"
                     /translation="MNSNFKVIDSHSHIYPEKIAKKASEAIGHFYGLPMLYTGTSSSL
                     IENGKKINAEKYLVCSVATSAAQVTVINDFIIDECKKHKQFIGLAAMHQDFIGVEEEI
                     ERTVNKGLKGIKMHHDFQKADLDDSRFFKTYKKCEKMGTVCLLHAGDDRYEYTRPYRF
                     VNILNKFPDLKCIAAHFGGYKRWEEAYKSFKDIDNLYFDTSSSLYALKPDEATDIIRT
                     FGVDKFLFGTDFPMWDHEKELERFLSLSLTNEENQMILYDNFIKLFDS"
     gene            complement(28494..28919)
                     /locus_tag="PLQ68_03480"
     CDS             complement(28494..28919)
                     /locus_tag="PLQ68_03480"
                     /inference="COORDINATES: protein motif:HMM:NF022338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HXK71745.1"
                     /translation="MEKKSKFRMNKSGMIITIILVLAIAALLIFSKVNVTVDDQSVNI
                     KSSLDRVTVDFVDIERVEKLTSLSVGSRVMGADLIRMYSGTFKNELYGRYKLFAYKDV
                     KEYVLIEHKGGFIVLNQETLELTEQLYETINSKFSAQAD"
     gene            complement(28957..30168)
                     /locus_tag="PLQ68_03485"
     CDS             complement(28957..30168)
                     /locus_tag="PLQ68_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004610190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HXK71746.1"
                     /translation="MNKLTDVSAKKENPKWENMISRKKEISVRENDIRSEFYRDYTRI
                     INSRAFRRLKQKTQVFFATRNDHVCTRMEHVNNVSSISFGIAKYLRLNEELIMAIAAG
                     HDLGHAPFGHSGERIINEISKQYLKTDFWHEKNSLRFIDYCETLQNASGQEDNLNLTY
                     AVRDGIVTHCGEIEKKQLMPREDFIELESINNASSVSPYTYEGCVVKASDIIAYLGRD
                     IEDALRLKMLDSDDIKSLDEILNQYKKAEETMNNTTIINSLVTDLCMNSSAKDGICFS
                     DEGFMMLKKVKTFNYEHIYKNARLKMYEDYAKLIIHSLFDKLMSFYRKNDTLAYIKAE
                     YSSIYPELASSFSERLKKYSDIPDRPLVYLNTVLYNLDNETDYILAVIDYISSMTDMY
                     AKKLFDEMTSF"
     gene            complement(30169..32058)
                     /locus_tag="PLQ68_03490"
     CDS             complement(30169..32058)
                     /locus_tag="PLQ68_03490"
                     /inference="COORDINATES: protein motif:HMM:NF014425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HXK71747.1"
                     /translation="MAEKKLEGFFGPKKIPNNPYMKKKTKNKNKQATREKLAILILTL
                     LYLVLALVNLGNTKGPVNGWTPQKAGSPVIVEFEQEETIERITFLAGLGDTWYNVTAL
                     DLYYKDQSGRYEYFATLEKGASKFLKWLYLNLEEPITTDSIKVVSGIYQDPEENFIRG
                     TRGEIMEVCFFAQGEQEPIAIKSLTPTNPYLDESATNLFDEQDLFVYEPSYMNSTYFD
                     EIYFPRTAYEFLIGNETIYENTHPHLGKIIIMWSYKIFGVNPFGYRAMGAIAGALMIP
                     LMYALGKKLFKNHYLAMVAAALLCFDCMHFVQTRLGTVDSYLVLFIMLTFYFMASYLD
                     MDLRKTSYIKTLVPLFFSGLFFGCAISVKWIGFYAGAGIAFLFFVKIYMEWKSFGYGR
                     IVHGGKALAARNTLLTCIICIFFFIAIPAVIYSVSYTPIFQIQSAAIDGQKFTIAEMA
                     DNIIASQKHMFSYHEGVTQDHPFKSSWYSWPIMYRPVYYYSAPIKAEGQWGTIVSLGN
                     PVIWWTGVAALIGTAIVSIKKRDKRGLFIFAGYLSILLPWAVAPRSITFLYHYFACVP
                     FLILAIVYMASYFMYGNSKIKKYVNLYVLLSALVFAAFYPAMTGVKVSVEYIELLRWL
                     PSWWF"
     gene            complement(32127..32765)
                     /locus_tag="PLQ68_03495"
     CDS             complement(32127..32765)
                     /locus_tag="PLQ68_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020875383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXK71748.1"
                     /translation="MLTDKIKWLGHASFRVDGSKTIYFDPFKIDIGKKADIIFISHKH
                     YDHCSKDDVNKILKEDTKIVTEAESAKILGLENMIIVKPYDKFAINGLEIEALPAYNV
                     NKPNHPKENGWLGFLVKMDGITIYHAGDTDLIEEMKKIETDVALIPVFPDDKYVMNPK
                     EAAKATFYIKSRVFVPMHYGIIGGSVQDAQKMKEHANPKCIVTIMEVQKNSF"
     gene            complement(32755..34023)
                     /locus_tag="PLQ68_03500"
     CDS             complement(32755..34023)
                     /locus_tag="PLQ68_03500"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HXK71749.1"
                     /translation="MDILIYIGILVLVILSFFFSGTEIAFTSLNIGRLRLESEAGDDK
                     KKKTYELSRDFSSVLYTVLVGNNLVNIAVSSLTTLLALRLLERTNSALPIKAITVAIT
                     TVVILILGEITPKLVANDNPNRFAVKMTGFLKIFIFIFKPVVFTVTKMTQKISKLWKP
                     RTTEPTMTEDELLSIVETIEEEGGFTEKESDLIRSAIEFSDDLAFEILTPRVDVFAID
                     IDEDPKEIIQKVLNEEYSRVPVYQGNMDNIVGILSTKKLLKAAVKKGALIDIKTMMKE
                     PLYIYKAMSISAVMEEFKIKRAHMAIVLDEFGGTMGIVTLEDVLEQLVGDIWDETDDL
                     EEEKIETEFGTYIVAGDMLLDDFFDMIGYEEEEYESEYTTVGGFATEMLGHLPKQGDS
                     FDFDGHIMTLLEVNNMRVEKVKVEARKDAD"
     gene            complement(34023..35366)
                     /locus_tag="PLQ68_03505"
     CDS             complement(34023..35366)
                     /locus_tag="PLQ68_03505"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HXK71750.1"
                     /translation="MDSDSIYRSITLIVLIVAGGLLAGMETAYSSCNKIRLKYNSENG
                     DIKSTRVLKIIEQSDKSITSILILLNIIHISAASIATVLAVRLLGEIGTLVSTVVLTL
                     LIFTFSEVIPKSIAKDNADAYAIAFAPVLKFISIVLTPLSYILSAVGRFMKKILGTNI
                     KEPTMTEDELQSMIETIEEEGVLQSDQSELIQSAIDFLDTTASDILTPRVDIVAVDID
                     WRKEKIDGTIIKEKYSRVPIYENTIDNVIGVLQTRKYLKGMLNNEAVDIRGIMDRPIF
                     VHQSMLVNTLFEMMSANRTHMVIVTDDYGGTAGLITMEDIMEELVGDIFDEDDIVEEE
                     YAYVEDGSLEIAGEMRMDELLDILDIPYNDDIIEHKTVSAWTMEHLQNVPNEKDSFED
                     FGLLVTIKELDGMRIEKLSIKVIEQEETEDNNENNKEKESKKKEKSKDNETEVKG"
BASE COUNT        10610 a         7333 c         5236 g        12315 t
ORIGIN      
        1 gaccggtttt tcgacagctt ctaaatctta gcacagcgtt tttactgtgt caacatattt
       61 ttctatttat tttgtaattt cttttcccct gtaaataact gacaatttta cacattgttt
      121 tacttgaatt tcatgctgaa tgacaaagaa aatccggctt ttgccggatt ctcttcgttt
      181 gtttttttta aattctttat ttaacttcta ttgttgtact tggagcttct tctttttctt
      241 tctttggcag tttcaccaaa agtactccgt tttcatattt tgcattgatg tcttcctgct
      301 taactccctt agcatagaag cttctggaaa attcaccata gcatctttct cttctgataa
      361 agttcttttc tttttcttcc ttctcacaat ctctactagc agaaattgta agatagttgt
      421 tcttcagttc aaccgatata tcctttttat caaaccctgg aagttcagcc tcaagaagat
      481 attctttatc attttctgat acatcaactt taaatccctg ggtactagtc ataacgtcgg
      541 tgctgaagaa gtcattcatc agatccaata cactgtaatc atttctttta tttcttgtta
      601 ttaagtcaaa catgcttgtc acctccatta actttgactt tccttgacct ataactattt
      661 tatcatatat ttttatcatc tcaaatccct attttgacct tatctgacca ttaagtgcaa
      721 ataaattaag atatctcacg atatctcttt ttttcttaaa tttgctgaat ttttcttaat
      781 ttttcttatg atactcagtt atatttcttt ttcaagcacc gattcaatac aatttacaac
      841 atcctgtttt gtttcagctt tgaaaatcat atctctaagt ttagcgctgt tatgcaggcc
      901 tttaaaatac caagcaccgt gcttacgcat ttccattata cccttctttt ccccattgta
      961 ttctatctga agatccatat gatgcttaaa tacttttatt ctctgttctg ttgttggaat
     1021 tatgtttgaa aatatccagg gattacctaa gcttcctctt gctatcataa catcaaaaca
     1081 acctgtcttt tttctaatat cttctatatc tttatgagta aacacatctc catttgctat
     1141 aacaggtatg ctaactgctt cttttaaact aataatatgg gtccagtcag cttcacccga
     1201 ataaaacata tcccgggttc ttggatgcaa agtgatgcat gaagctcccg cagattccat
     1261 cgctttacca aattcaacat aatttagatt atgcttttca aatcctgaac ggaattttac
     1321 gcttatagga actgatgccg catcaacaac tgccttggtt aatttaaaag ccttcttaat
     1381 atccttcatt agggcgctgc cttcatggtt ctttactact tttgagacag gacatcccat
     1441 gtttatatct atcaacacag gatttaaaga ggatatgtat tgtgctgcat actgcatagg
     1501 ttcaatttca gacccgaata tctgaaccgc aaatggttca gaaggattgg ttttgcttaa
     1561 tagtttctta gttttttggt cgtcgtagaa taaacctttt gcgctaatca tttccgagta
     1621 agtcagcaca gcaccatgtt caatacatat tgttcgaaat ggcgcgtcag ttattcctgc
     1681 tattggagct aaaaaaagcc cctttatctc tttaccttgt ataatcaaaa ggttttatcc
     1741 tttcatatat tattactata tattatatac taaaactctt ctttatcaga tttcgtttcg
     1801 caatacgcgc atctgtaaat cctattttcc ctatcagtta gattaaaaac ctggtctatc
     1861 tcctgttcaa ttgaggtaat acatcttgga ttcttacact taattacatt tacaagttta
     1921 actggcagct ctagatgctt tttctctgat atctttccat ctcgtattat gtttacagta
     1981 atattaggat ctatatatcc aagcacatcc agattcatat caattataga atctatttta
     2041 ataacatcct taataccata cttcctgctc ctgcaatttc ttataactgc cactgaacat
     2101 ttcaattctt ctaaattgag atatttatat atcctcatac tgttacctgc tttaatatga
     2161 tctagaacta ttccattaac tatgctgtct atcttcatgt catcctccac tatactaaaa
     2221 tatccagaag cattaatatt agcgccatgc gtatatattt tccattttga acctgcttga
     2281 aataacatgc cctgggatca tcatcaacct taactgaaat ctcattcact ctcggaagag
     2341 gatgcattat acttaaatcc ttttttgctt ttgcaagctt atctatagtt aatatgtagc
     2401 tgtctttcaa tctaatatag tctgcttcat taaagaatct ctccctttgg actctggtca
     2461 tatatagaat atccagtgaa ggcatatact catccatctt gtttgtttct atatatttta
     2521 tattattttt atccagtata ttagccttca cataatctgg tattactagt tcctttggtg
     2581 atatgaatac atactcaatt ccatcatatc ttgaaagggc gtttattagg gagtgaacag
     2641 ttctaccgaa tttaagatct ccacagaaac ccacagtcat atttgaaagt cttccttttt
     2701 ccctatgaat agtaagaaga tcagcgagcg tctgagtcgg atgactatga cctccgtctc
     2761 cagcatttat tataggaact gtagttcttc ttgaagcagc aagcggagcg ccttctttgg
     2821 gatgtctcat tgcgattata tctgcataaa aacttactgt ttgtactgta tctgtaacgc
     2881 tttctccttt tgaagatgaa gagctgtcag aagtggaaaa ccctatgacg cttccaccca
     2941 attccaacat tgccgcttca aagctcagcc ttgttcttgt actcggttcg aagaaaagtg
     3001 tagccagttt ttttctttta cagatatcct gatatttttc aggattaact attatatcgt
     3061 ttgcagtgtt tataaggtca tcaatctgtg atgtcgtaag gtcagttata tccatcagat
     3121 gcttcattgt ggtctcctta tgcctctatc catccttcat cagaaggatt atcccgaaat
     3181 gttaataatt tatttttgtc attctgcttt atatatcctt tttcgcatgc tacctcaata
     3241 atcgtatcaa aatcggtcag cgaaatattc tgtacattcg tctctttaag tctttgtacg
     3301 ccttttgcca tcccataagt gaatatactt gctattccaa gcacttgagc ttcttcttct
     3361 cttaatgctt caacagtatc tataacactt ccagctgtag atatgagatc ttcaattacc
     3421 acagtcttct gaccgggtaa aagtcttcct tcaatacggt ttcctcttcc atggtctttt
     3481 gcagaagaac gtacatagcc catgggaagt cccagtatat gtgcagtaat agcagcatga
     3541 gctattcccg ctgtgcttgt tcccattatt acctcgcatt gaggatagtt ttttattata
     3601 agctctgcaa ggcctttttc tatatcagcc cttaccaaat ggtcagataa tgtaattctg
     3661 ttatcacagt aaatcgggct tttaattccg cttgcccaga caaaaggttt gtcaggactt
     3721 aaaaatactg cttgtatatt aagtaaatgc tcagctatct ttcttttcat tgggtttcct
     3781 ttccgtaaac aaattcttct atgcaccttc tgtatacccc tacagggtca tcagattgcg
     3841 ttatcggtct tccgaccact atgaagtcac atccaatctt tgaggcttga tatggggtcg
     3901 tgatgcgaga ctggtcatct tgtctactgt gtttaaatct tattcccgga gttacagtaa
     3961 caaatgattt cccgcatgta ttctttactt tttcagcttc caatggtgaa caaaccacac
     4021 catctattcc ggctgtcact gcaagctttg cataatgcat aactgtatta tccattgagc
     4081 tgttgatcat taattgctca tgaagctccg attcatttgt tgatgttagc tgagtgactc
     4141 caattagaag aggtcgggtt ccatcttctc ttgtcagtcc ctttaatgct gcctgcatca
     4201 ttttcacagt accggatgta tgcagattta ccatatcaac atccaataac gacaagcttc
     4261 tcatggctct ttcaactgta tttggtatat catgaagttt caggtctaaa aatatcttat
     4321 gtcccttttc cttaatatct cttactatat ccggaccttc tgcataaaaa agttccattc
     4381 caatttttac aaaaggtttt ttctgcccaa acaagttcag aaaatccatt acatccttct
     4441 ttgacggaaa atcacatgca ataattacat cttttttcat taatgcgccc ctcctataat
     4501 ttcatttata tccttaactt taagtctatt aagtctttca ggtaagtctt caataattgt
     4561 cttacatata catggatcaa ttaaattcat tgaacctatc tgcacagctg ttgctcctgc
     4621 catcatcatc tctattacat catcagtgct gcttataccg cctataccga ttattggtat
     4681 tttaacagcc ttatatatct gataaaccat acgaagagct acaggaaaga ctgcaggtcc
     4741 tgacaatcct ccagttatat tagctaacac aggctttctg tttttaatat cgattctcat
     4801 gcctaacagg gtattaatag cgcatatacc tgctgctcct gcttcttcgc atgcttttgc
     4861 tattgatact atatcagtca cgttaggaga taatttaata tatacaggtt tgtcagcaac
     4921 cttcacaact gcttttgtca ctttagcagc catatcagca tttgttccaa aactcatacc
     4981 tccctcatgc acattgggac agcttatatt tacctcgatt agccctatat tatcgatacc
     5041 tcttaagctt ttcgcgcaat attcatattc ttcaattgag aagcctgata tattggctat
     5101 tacgggtttg gaatagtatg atgaaatttc ctttagaacc tgtctcacaa catgcaaaat
     5161 tcctggattc tgtaatccta cgctgttaag cataccgcaa ctgctttcag caatccttgg
     5221 ggtatcattt ccgaatctgg cttcttttgt tgtccctttt gtgcagaatg agcccaagat
     5281 gttaatatcg taatactgta tgaattcttt tccgaatcca aagcatccac ttgcaggtat
     5341 tatggggttt gataactcca atccgcttaa gtttactttc aaattcacca tattatctcc
     5401 tctttgaata agaccggacc atctttacat atccttttgt atccatactt agtcttgcat
     5461 gtacacccca tgcatgcgcc gaatccgcaa cccatccttt cttcaaaaga aaactgtcca
     5521 ggttttgaac tgttctcata aacagctttt agcataggtt caggaccaca ggtgaatata
     5581 tagtcataat caatcatact tatagcatct gttgcaaagc ctttatatcc ataacttcca
     5641 tctacacaac agacatatac cttacagcca atgtctgcaa attctttttc atagaaaaca
     5701 tcattcttct tgttaaatcc aagaattaca tttggaatct tgcctttttt aataagtgtc
     5761 ttagcaagat aatacaaagg cggcacaccg catcctccac ctatcaaaag aggctttaag
     5821 gtaaatactt ctgtatcata cccattacca agattacaca atatatccag ttgctgtaat
     5881 ggtttcatac aagacagtat ttcagttcct tgtcctacta ctttgaatat caaggtcatc
     5941 ttatcatcag ctatatcaca taccgataaa ggtcttctta aaaaaagacc atcaattttt
     6001 atattcacaa actgacctgg cgcccttatc catgaagtat caccggataa aaccatctca
     6061 tatgtatcat atgtaagctt cctaatatta tttactgtgt atattcccat actatttttc
     6121 ctttgcagat tgtttttata actcgtccat tcagtgtcat tccttcaaaa ggagtatttc
     6181 ttcccaaaga cagaaatcca tcagatttaa ctgtattttc tgcatctaaa tccaacactg
     6241 taaaatcagg atacttaggt attctgaata tgcatgaagg atttactgac ataagctcaa
     6301 taagtttttc cagttttatg attcctgttt taacaagata tgtgtacata ctggaaaagg
     6361 atgtttccat tcctatgact cccattgcac ctgctaattt atcttcatat gtgtgtgggg
     6421 catggtcagt tgcaataata tctattgttc catcacaaac tgcctgaatc aatgcttctt
     6481 tatcttcttt gtcccccaaa ggcggattca tcttgaaatt ctcagatatc acatcctctt
     6541 ttgtaaagac aagataatga ggagcagtct cacatgtcac gctgattccg tctttttttg
     6601 ccttccttat taactcaata ctttttttag atgatacatg acagacatga tatctgcatg
     6661 aagtatccgt attttttagc aattcaatat cccttttaat attcatatat tcactcattc
     6721 taggatcttc atatgagaag tcttcacaat gagcggcaat aagtacatca tgcatagccg
     6781 attgctcaat cattttcttc attatcaaat cagatgaaac agggtttccg tcatctgaaa
     6841 aacctataac aaagtctttt agttttgaaa catcaaccag attctgtcct aatctgcctt
     6901 ctgttatgca gctgtattga tatacattga tacatgcatc cttgtcaaca atatcctgta
     6961 tcaacctgat attatcaata tcatcaggtg atggggtaac atttggcatt gcacatatcg
     7021 acgtaaatcc tccagctttg gcactttcag agccagtcaa aattgtttct ttgtattcaa
     7081 atccaggctc tctgagatga acatgcacat caacaagccc tggaacaata tatttttttg
     7141 aaaggtcaat aatctgatca cactttatag atgaaaggtc ttcaccgatt atccactcgg
     7201 tttcattgat aatatataaa tcggaggatt tgaagcttcc atctatatat ataaaagcgt
     7261 tctttaataa catgacatcc tcctgttaaa taggctgccc ccatttaaag agggcagcca
     7321 taaatgcaat tcgttacttg ctttctgctt tgtctttctt gtcagcaggt attacaagat
     7381 ttaatattat acctataagg gttgctactg caagaggaga aatctctact gcaccaatca
     7441 taagtttgta tgctgtatca ccagttacat ctcctgcaag actcatagca cctaaaccaa
     7501 gtcctactga aagaattact gatactacta atatattctt agtatctgag aaatcaactt
     7561 ttccgtcaac taatgctcta agaccagctg cagatatcat tccgaagagt actattgaag
     7621 ctcctccgat tacagcagat ggaattgtgc ttactgctgt accgaatatg gttatggaac
     7681 cgaatataac tgccattact gctgcataga agatgtttct tgggttatag ttctttgtta
     7741 ttgcaagaac tgctgtgttt tctccatatg ttgtatttgg aggtccacca agtgcgcctg
     7801 ctatgaaggt tgcaacgcca tcgcccatta ctgttctatt gataccaggg tctaccatga
     7861 agtctttgtt acataccgtg ctgtttgctg atatatcacc aagatgctcc ataaaagtaa
     7921 ctatcgcaat tggaacaact gacagtataa ctcctaaatc aaaagacaga ttcttataga
     7981 aactgaatgt cttgcctaaa tcctggaata ttattatctg actccagtca actgttgaat
     8041 aatctacaag accaattatt aatgagtaga tatatccgac aacaaatccc atgagaatcg
     8101 gaattacttt gaagaaactc tttggttttg ctaccgcgct tactacaaga attgttaaag
     8161 ctgtgattcc tgctgctgtc cattctttcc atgctgggat attattccat atggattgtc
     8221 caataatgtt gttatagaac atcttaggtg caagaatcat acctataact atgataaccg
     8281 ggccaacaac tataggcggg aataattttc ttattctttc tacgcctatc tttttaagca
     8341 agagagagaa tatgacatat acaagacctg ccatcataat agctacgctt gtttttccca
     8401 ttgccacatt ccatgtagct gagccgatat ctccagcacc tgccatagat gctattagag
     8461 ccggcaggaa ggcaaaactg cttcctaaaa ataccggaac tttcttcttt gttataaagt
     8521 agaaagctat tgttcctatt cctgctgaaa gcaatgccac cgaaatgctc attcctgcaa
     8581 gaatcggtac aaggaccgtt gctccgaaca ttgcgaacat gtgctgaaag cttagcactg
     8641 ccgttttcaa tgtgaagcgt tcatttactg tttttcccat tgttatttct cctcctagaa
     8701 aaatttataa atacttatat acgataaagg ctcacacctg ttatcccgtc tgtttccaaa
     8761 agcttcacag caatcatctc gcccttggct gatggcacat ttttgcctac gaaatccggt
     8821 cttataggaa gttccctgtg tcctctgtca attagtaccg ctaacttaat ctttgcagct
     8881 ctgcctatag agaaaattgc atccatagct gcccgcgcag tacgtccggt aaaaattacg
     8941 tcatcgataa ggatgacgtt tttacctgtt attttaaatt gtatgtctgt tgcgttaagt
     9001 atcggatctt cagagcattt ggataagtca tcacgatata atgttatatc cagtatccca
     9061 actggtacct ttactcctga aatgtgttca atattttttg ctattctttc agctaatgga
     9121 attcctctgg ttttaatacc tactagacag atcccttctg gacccttgtt gtcttctaag
     9181 atttcatgtg atatacggat taacgatctt tttaccgcat cttcatccat caaagtagct
     9241 ttgaactcca cattcgcctc cttgcataaa tttctaaaaa aaatccttgt ctgcgcatgc
     9301 atacaaggat agatgaatct tgttaattct tctttataaa caccttgtgg gtatctctgc
     9361 accgcactta aagttctttt aatatagtac caaatatatc ttgatatagc aagtagtttt
     9421 agtaaattaa ccttaaaaat ttaacgataa ttaaaaatca aatcccttac tattttatct
     9481 ttactgcagt accatacgct acgacttcag cagctccctg tacaatagca gcagatacat
     9541 atcgaatacc gataatagca tcagctccca tcttttctgc ctgctctaca attctttttg
     9601 tagccattgc tctggattcg ttcatcattt cagtataccc aaacagctcg ccaccgacta
     9661 atgttttaaa acttgcagcg atgtcttttc cgatattttt tgactgaacc ataccggctt
     9721 gcactaatcc taaatcctca atttcttttc ctggaacata atcaattgtt aaaagtttca
     9781 ttttttctaa tagccttctt ccaatgcttt agcagcagga tccatcggca tctcctttat
     9841 tttaaattta tttttaaact taataatata tatagctgaa ataactgtaa acacaagcaa
     9901 gactgccaaa ctgatccatg tattctgtgt taacatgctt actgatacta ctgtaaaatc
     9961 gagtaaagta tgtaaaacta tacaaagtac aaatccagca aaagttcttt tctttgtcat
    10021 catatatgcc agaaaaaccg taagaccaat atggaaaata accgtaagga ttctttctat
    10081 accagctgca tagaaaagtg aagatgatgt agttaagaaa gtatctttga tggattcata
    10141 ttgttcacct aaaggaagcg aacctgtatt aatcataatt gcgaatataa tgttattaat
    10201 atatgtaagt cctataagac cgactgcttc tgctgcacca tgcccatatc cagctcccag
    10261 tgaactgtaa tatgatagtt cattttttga aagaacctta agcacaatca gtcttcctgt
    10321 tgtttcaaat aaaccggcag agaaagccag gattaacaga aaaaagtact tattatcctt
    10381 gctgaactgt gtaactgatg gatttgtact aagaagctgt aaaacaggaa ttcgtataaa
    10441 cagctgcggg attataaatc ctaaagctcc cgctattatg gcttttgata ttccttttac
    10501 cttttttgca aaaactatcc agattacaac aggcactatt aaagtaataa ttagagtaaa
    10561 tactatacct gtgattgctg atgctgatac catagctgcc ccttttaaat taagtaaatt
    10621 ccgtctttta ttctataata tttttataaa aataaaaacc cccaaaaatg agggtttctt
    10681 ttaggaaaat tacgaatatt aattatgctt tcataggtgc ttttctcatc gtaaaccatg
    10741 acagaagtcc ggttattaag aatattacta aacatgatac aacagctaaa tatggatttt
    10801 gtgcggagat cccaaatatt atgtccataa accttattat tccgatagtt atctgtccat
    10861 caaaaatcaa tacatccact actggtaaaa ttacagtgaa aaatactggt attccaacag
    10921 ccaaagcaac tttcccaatc ttgttaagcc tgtagaaaat aactcctatt aagtaaccta
    10981 tgcttactat cataagcagc attacaaaat acaacagtaa cgactcaaga aaaacaacaa
    11041 tatcattcat gccagctaca tgacctatat agattctttc aaaaaaactt tctgatatta
    11101 tattgctctg aaaatgtgac atcagctttg ctatcaacaa aactaagata cttattacag
    11161 acaacattgc agatgatgta acgaaaccta acattgtggc agtaaaagta ccttttctgg
    11221 atacgctgtt ttgtgtcagc atatgaaagt tttctttgaa ggctgtaagt cctgcaatga
    11281 atatgaaaat cattgaagcc atttccatcc cgcctatctg cattgtattt gagtcattgg
    11341 accatgatat tgttgcaatg aacagcaata cagtaacagc tatgattata agcgaatatc
    11401 caagtagtgc ttttttgtta tcattcataa catatttata tgatgctctt aaattcattt
    11461 tgataccccc ttctcatttg tcatcttaat gaacatcttc tgtaaatcca tttttgtaac
    11521 ttcaatattt tcaggtatat ccactggagc tttacctgat acataagctg ttttcaaacc
    11581 gccaagcatg tcataactca ttacatcctt atcagctgta aataaatcaa cagcatttgc
    11641 aggaccggtt attgtatatc cttgtgacaa tatgctttct agaggttcat cttttattac
    11701 gactccctta tctatcatta ttacatgttc tattatggat gatacttcat ctataagatg
    11761 tgttgagaca actgctgtaa atggtttctc actatacttt tctattaaaa gcctgtagaa
    11821 caggtctctg taatttgcat caaggcctag tataggttca tcaaaaaata catattgaac
    11881 attaacactc attgcagtta taagctttaa aatcgagcca tagcctgtgg acaatgaatc
    11941 tcttttcttt ttaaggtcca gttcaaactt tgatgcaagc ttcaatgcat aatccatatc
    12001 aaaactcgga taaaatttag aagtccattt aaatacatcc ttaaccctca ttttttcagg
    12061 gaacagactc ttctcactca tcatgtatat attggataaa gcatcgtcat tttcttttat
    12121 gggctttcca tccaatagca cttcgcctga atctgcaaac agcctatttg taataatatt
    12181 cagtaatgtg gttttacctg cgccatttct tccaagtaat ccataaattt tgtctttctc
    12241 aaaattcaca ctcacttgtc ttaaagcctg agtagaacca taacttttac ttaaaccctt
    12301 tacttcaata ccactcattt ttcataccct ctttctatca tttccataat atcttcctta
    12361 tttagattta atttcttcgc ttcatctaaa agcactgcta tatagctttc gtaaaacttg
    12421 ttctttcttt ctttcataag tttcttaaca gccccttcct ttacaaacat tccaagtccc
    12481 cttttcttgt aaacaatttc attatctact aacatattaa tgcctttcaa agccgtagct
    12541 ggatttatct tacacataac agatatttct gttatggaag gaatctggct ttcttcttca
    12601 aacgcatttg ataatattgc atcttctaac cactctgcta tctgaacaaa tataggctta
    12661 tctgattcaa aatcaatatt cacggcatcc tcctttcgta tcttagttaa ttacttgact
    12721 aactaactaa ctaagataca ttttacacca taaaaaatac ttgtcaatag gtaatttaaa
    12781 attttattca caattcaact taactttgac ttttttacgg ttctttaaaa tatcacttct
    12841 gcttctaatg aaaaaaggct atatgcaagc atacagcctc tggcgtcccg aacaggaatc
    12901 gaacccgcat ctgaggaacc ggaatccccc gttttatcca ttagactatc gggacatgag
    12961 agtaattttc attactctgt atattaatat attaatagtt attaacaaca tccatggcta
    13021 tattagccca tcttgtcaat ctttctggtt cgcattttac tgtagatatg tcttttagta
    13081 tcatttcaag attaacatta ttctttctgg ctgcatcgac agtcctttga agatctcttc
    13141 taatttcatc ctcatccagc gaagctgttg caaccagtgc aggagtcggt ttattgctca
    13201 taattacttt tggtccgatc ttttcagcaa atgcttctct tactgaccat gggctgcagg
    13261 atactttctt taaatgagga atagtaagca ccaaatccag tctgtcatcc aatctgtcgc
    13321 aacatccgta atatatcata ccgaagtact cagccattct tttaatgtaa ggaagctcaa
    13381 actcttcggt aactgaggga gatacagatg agaataactg agctaaaccg aatgcccatc
    13441 cgtttttagt tattgtctct tttcccatcc cgctgtctgg taacagttca tctgtgtata
    13501 tatatgaaca gtggcaggta tttgcattag tatcatagat tttcagtttg ttcgactgct
    13561 ctattccttt tataactgat gtagttatac gctccataaa aacatgaagt ttttccggct
    13621 catctacaag gtcaaaatat atattctcaa ctcccatgaa ctgagacaag tgatcccata
    13681 ctccaagatg aaacatattg ccttccatca taacaggtgc aatacctttg aatatttctg
    13741 aagcagtctc ataccttctt ttagtttcct cttcatcatg agtaataacc atatccttaa
    13801 tcttattagc atcttcttct ttaattaact gatttgtata aacatgagaa cttacattac
    13861 ctgaagcgta caaagattct tcttctactt ttaaaccgta acctgtatta ctaattgcct
    13921 ttggtactcg aataaaagga tctagtacca tatctacagg ataatgcttg tatttatata
    13981 cctctctttt aagataatat tcgaccctcc taaatacggg gtcttctaca aaaagatcta
    14041 attcgtgttc attgttcatt tcattccatg gaatctgatc tataacaacc ataggccttt
    14101 ccattttgct tctatttaaa gccttccaga gcctgatctt ttctttctga atcggcagtg
    14161 ttgcatattc catatattgt ttcgccatct cctgcaagac tgatatttct tttttagtaa
    14221 gttccataat gttcgccttt ctgagtaaat atattggtca attaaattat accttaatac
    14281 tttcttttgt atatgtcaaa cgtaaatatt tatgacaggt atatgttatc ataaaatata
    14341 tattccgttt atttacatta aatttgtttt cattttatta attaaaaaat caggactaac
    14401 tagtcctgat aatttattct taatttttta cttacttatt tctattcctt ttgcctattt
    14461 atataaaggt ccaaaattgt tgcatgctct gtagtcagcg ccttcaagat cattagtgcc
    14521 gaacgatgac catgcgcttg gtctgtatat cctgtcattt tcaatattgt gcatattaac
    14581 aggtattctt agcatcgagc ataaggttat gaagcggtct cctacatgtc cgtaagtaag
    14641 cacgccatga ttagctcccc attttgccat tacttcatat acgctggtaa atgctccttc
    14701 acctgtaagc cttggtgcga aatatgtagt aggccaggta ggatcagttc tgtcgttaag
    14761 catctcatga gcttttgggt ctatatccac tgtccagcct tctgcaatct gaagaacagg
    14821 tcctaaacca tctacgatat tcatccttgt cattgtaaca ggcattcctc cctttgtaag
    14881 gaaggttgat gaaaatccgc ctcctctaaa gtatccaaga ttagctggac cccacgtggt
    14941 gttatccaga catgcctgag cttcctcttc agatatttca tagaatggct tcattacagg
    15001 ctttccgtct tcttcacatc ttcctgttcc atccaaagct gcagctccag agtttataag
    15061 gtggattatg ccatttttcg catatccttt taatgtatgt ccggtaactc tcttaacagc
    15121 ttcaggactc cagtatgttc ttacatctgc aaatatctga gccgttcccg aagtaagaag
    15181 atgtccaaaa agcattgctg tcccattaag tccgtcattt tctgtagcaa caatgtatgg
    15241 ctggcgtatt cctgtccagt caaatgaaga attaaggatt gcttccataa agtctccgtt
    15301 tggcataaag tctgtccact gtctctgccc ctggaaacct gccataattg catttcttcc
    15361 gcatccttct tcttcaaatc cgttcttgtt cagataatcg cttcctgtca tgatgtcttt
    15421 agttataatt gccatcttga cacaagtctc ccactcttta tctttctgct cacgagtgtg
    15481 aggtgtctta ttgaaatcat ctccttcttt gcagttcttt ttcgtccact ctagagctgt
    15541 tttaaactct tccttatcat aaatgccctc attcatcctg cggataaatt ctgacatgtc
    15601 tatgtattca tttcttattc ctaaatattt ctggtagaaa ttaggatctg cgattgaacc
    15661 agctataccc atggaaacgc ttcctaaact tacataggat tttcccttca tatatgatac
    15721 tgccaaacct gctcttacaa aattaagtat cttatctctt acatcatctg gtatttcatt
    15781 atcattggca tcctgtacat cttttccata tattccgaat gcaggaagtc ctttctggct
    15841 gtgtgctgca agtactgctg caagatatac cgcaccagga cgttcagtac cgttaaaacc
    15901 ccaaactgct tttggcatat gaggatccat atccattgtc tcgcttccat aacaccagca
    15961 acgggttaca gttaacgtca aaccaactcc ttctcttgcg aacttctctg cacatgctgc
    16021 tgcttctgct acatttccaa tacatgtatc tgcaacaacg cattccacag gcataccatt
    16081 aggatacttc agattttgtg aaattagttc cgctgcggca taagccatac ccattacaac
    16141 aggctcaagc tcttctctga cgcctcttct tcttccatcg attgtcggcc ttatgcctac
    16201 ttttggcaaa gtgcctttca atctttccac attactgttc atgatcattt cctgtaccat
    16261 ttcctttcgt atatattaat tgatagttac atttaccagc tggagcctcc accacctccg
    16321 ccgcctccgc cggagaatcc tccggaagag aacccgcctc cgctacttgt tgatggagct
    16381 ttagccatag aggctgttgt ctgatttaga cagctgttca ttcgagacaa taagtatact
    16441 gtcgagaatg ttctcatgtt atatccatga taccactgtg gaggctcaac tactatgctt
    16501 tcaaactttt taccccattt cttttccaaa ccaagtacta ttgcataagg aaggacgtta
    16561 tagaagtatt tcggattctc gtcaattaac atctttatcc tgtccatttc agctgttgca
    16621 ataaagtctt taaatccaag ataatgctcc atcattttct gaccatactc agatctcttc
    16681 ttcattgcca tagagtatgc caaagctatg gctgtaataa aagcgattat gaaatacgct
    16741 attgaataag ttgtaacatc ataatatggg taaattgagg caagcatcaa atctgcgata
    16801 cttgcagata ttactaaaag tatcgtaacc agtaccgcaa ttagaaattt cttgaaatta
    16861 ccagttatat tccacttatt ctctgcagaa acatacagtc ctaaaatcat accgtaaaac
    16921 ataccataaa aaatggttaa tggtatcata attgcagctt ctttaaactc aatccatgaa
    16981 attatgagga atactataaa aggtattacg gtaataagag ctgattttgc ccgattctta
    17041 agagagtctt tggataccag ttccctgtct tttctgaaca catactttat tttctgcttt
    17101 ataactggta tcgcggtata gaaatggtct ttcaagtcgt ttgtcgaaac agtattattc
    17161 acaccatatt caaaaaactt gtcaaacata tactgctcat attcattgtc gaatttcggt
    17221 tcttgaagtt ttgtgaattc ataactccat ttcttatcgg taatttccag atatcctttg
    17281 tctgcccagt agaagattaa tgatgttatg tctttattat caataacacc gtcaattata
    17341 tatcccgctt ctgcaggaga ttgtcctttt accggataaa attcaggtgc agcaaaaagc
    17401 tggtcatctt ttcccttttt ataccactgt aaaacacagc aaatcagtac cagaaccgct
    17461 atcggtgctg ttatggctat aacaggaatg tctataccgt ctataggacg ttcgtttaca
    17521 aaatagcctt caggtaattc caatgcaagc gtaactccct gatatgaata aagatcggaa
    17581 acttcgcctc ttatagtatt tccagtaaca gtataatcaa catattttga tgtactgtat
    17641 ctgtctccgt atgtaatgga tatattgtct ttatcaaatg attttggcat attaactgca
    17701 aatgtaacat tatttacatc gcaatcccag tcagtaccca gtatattatg ataaatctta
    17761 tcgtaattct cattaccatc atccccataa tcatagtagt aggatatggt atatttgacc
    17821 tctcctttta catacctgtc tgcatcacca atacgtatgg caatatattc tcttgatgat
    17881 tcaacagaat aagtcgtatc aacatatatg ttatctatat gtacttttct gttctcaaaa
    17941 acagtaggaa ttgtacgtat tatcccatgt tttccatcag cagtgaaaac agctgtaata
    18001 ttctctgtaa tgtatagtac attattctcc tgaacatcaa taatcgtatc ataactggat
    18061 atatgataac cgttaacatc atacgcataa acgtgcgaaa aagcgcatat cagtaaaact
    18121 gacagcgcta taagtgctat acttctataa ataacaatct ttttcattct aattaaaaac
    18181 tcacttttac gttctggcgt tcttcttcca taacttcata ataaggctgt ttttcaaatt
    18241 tgcagattga agcgactata gaacttggta ttgtctgagt ctttgtattg aaattcttga
    18301 ctatagcatt gtagtacttc cttgcgcttg ctatttctga ttcaatacca gaaagctgag
    18361 actgtaaatc caaaaagccc tgatttgctt taaggtccgg ataattctct gctattgcaa
    18421 agagactttt caaagcacct tcaagagctg tttcatttgc ctgagactct gctgatgtct
    18481 tgctctgcat tgcattagcc cttgcttcaa caaccttcat aaatgtctgt tcttcatgct
    18541 tagcataacc tttaactgtt tcaacaagat ttggaatgag atcgtatctt ttcttgcaat
    18601 atacgtcaac agtcgcattt gcttcttcaa acttgtttct taatgagata aaactgttat
    18661 atgcagctat aaaatatact gccgctaata gtattacccc taacactaca taacctaata
    18721 ttgtccaacc cattttattt acctccgttt gactcaatta tatattactt atattcatca
    18781 tacaaataat attttttgcg ttttttgtgc gtattccttg ttttttaaat atttttagct
    18841 ttaaacgcca atatattaaa aataccttct ttttgatgtt gacgaattat tcctccatgc
    18901 tataattcct atgaaaggcg actaacacat gaagaattat ttcaccttaa tatccaatct
    18961 ggcagacaaa caaatacacc catcggtgaa cgaggcaaca aaccaaaaag taataataaa
    19021 gaaaaatgag acaaattgtc cttcttcata cggcattaaa aaagccgtca tttttttaag
    19081 cgcttgcttt aactcatttt ctgcttacta taaatcaccg aaattgatga aacagacact
    19141 tgaaagcata ttggccgcta cgaaatatgt aactaaaggt tcatatgagc ctgtgtcatt
    19201 ctgcatatca gtaatacagg cctccaatat tttcaggcta gcaaagacat atgcaaaaat
    19261 acttagcagt tgtgataagc atttaggaaa tgaaattcag aatgctgctt tggattatat
    19321 ggaaaaatct aaagatgtct taatgaacat caatacagat aatctttatc tttattcagc
    19381 tctatgtcgc ttgaaaaaag atttaaaaga cgaaattata actaatgctg ctgataatat
    19441 taaacaatca gtaacaaatc tttcaaaact agataatgta tccatatgtg ttctttgcga
    19501 tatttaccgg gaaacaaagg ataaatcata tttaaaagca gctcaaaaca atatggaaaa
    19561 cgtgcttaag cttttagaac ctgatgatac agtaatttca atagaagatg aaaagaataa
    19621 tcttaaaaac agaaaatatc cattatgcat acaggattat ttaggccctt tattatatct
    19681 ttctttagtt tcaaagaatg agcagctgga caaagctctg ttttatgcaa ataaagcttt
    19741 agtagttgat gagaatccag atatattcga aagcgagaac cttttgttta ttccttcatt
    19801 ggtattgtta aataaaggac ttttagatat tgaaatatct gatattacca gagttgaagc
    19861 attatcttca tttagcggtt atgtttctga ttttggtata tacagacacg gaacaggtac
    19921 acagatatca cagactattt acaaagatca ggaccttttc tattctatcc aatcaggagc
    19981 tttgaaatta agactcaggt taaatgctac tttctttggt cctaaaggca gatttaaaag
    20041 tactgatata gaaaaaacag aaaaaggaat tcgtctgcat tatcaaagac agtggggtta
    20101 ctggcttccc ttagaggaca aatccgttcc gccaattata ggaagcgata ttaataagat
    20161 tgaccgtaaa atgataaggc ttcaggattt tgatgttgaa gtatacatag acttattgaa
    20221 agatggcgct gatataacca tgactttatc aaagacagat gaaataccct gcaaactaga
    20281 ccttatattt gataaattcg gagtttttga ctctgattct gcatcaatta ataccatagg
    20341 taatgactac atacagcttc aaaatggata cttttcatat tcttcaggtt ccgattatat
    20401 aacagctggt cctgcgttta acggttcatc tatttacagc gagaccttaa gaggcagtaa
    20461 tcctcctatt gataacacat ttactgtata ttttacagac atgacaccta ctactagaac
    20521 aatatccata agaggaggca aagcatgaaa gaacttatta aagaacgtat ccatcgttca
    20581 gacgatataa tcgaaaatct tatagaaggg cagtgccttg ataaaaactc attctattat
    20641 ggagcagtca ttgatcacac cacaggttat gcagaacctt cacggggaat atcaatagcc
    20701 ggatatttca tattgaaata ctgccttcct gattcaaagt tttataatta tgatcttttt
    20761 ctggaaagag caatcatagc aataaagtat tcattaaaga gacttcatgc tgataatact
    20821 tttgatctta catgcactaa tccacatgat tctacaagca ttgctttctc agtaagaata
    20881 gtagctcctg cattaagact tttaaaaaga catatggaaa agaaagacga tgttaaaaag
    20941 attgaaattg acgttcataa tgccttggtt gattttctga ctaaatcagt tgacggaatg
    21001 gtgggaaacg ggtttcacac accaaatcat cgctgggtgg tagcaagcgc gcttgctctt
    21061 gatatgaata tccttggaat gcctgaactt atggatgaaa taaacaaata ccttgatgaa
    21121 ggtatcgatt gtgatgagtg cggtgaatat gctgaaagat ccatatctat atataatctt
    21181 acaaataatg agtctcttat aatcttagct catgaactta acagacctga cttattagag
    21241 catgttaaac gaaaccttta tatgactaca aaatattttg aaccggatgg aacattgttt
    21301 acattaaatt caacaagaca ggactttggc acaaaaacat atccatttgc tctgttcgaa
    21361 agttatatgg taatggctca tttagataat aactcggatt tcgcatatat ggctaaacat
    21421 atattcgata tgtccagtaa taacccatat gatattagcg atcaaggata ctattccttt
    21481 gcaggaagat tcgttcagca catggaatat actgatcatt attctggaaa cgttgaaatc
    21541 aaaccctttg acttttctca ttatgacagt ttctttccta attcagatat agcaaggata
    21601 agagatgacg acacttcaat gaccatactg gctaaaaatg taatgaccat gaaatatcag
    21661 tataagtttt taagtattca tatgatgtgt atggctgatg gatatgtaga atgtgaaaaa
    21721 attgaaaaaa ctgataaagg ctatcgtctt gtaagtcatg acaagaagac agatataacc
    21781 ttggattttg agtttaaaaa agacggagct gaaataaagg ttgatgctaa aagtaaccag
    21841 ccttatcctt gtaccatagc tttgatgttt gacaaagaag gatggctcag ttctgatgat
    21901 tattcgcttc aggcaagaat gggtaataac atatttatca aaaacaaaaa gttcaaatac
    21961 tcccattctg taaagtttta tccttattca ttatttatag aaactgattt tgaagatgaa
    22021 ggttttgcag atttagcaat aagcaaagta atgcctacag ataaaaacgc ttttacggta
    22081 ttattttccg cagaagcgcc ttttaataaa actatagtta taaagaagag ttaatctatt
    22141 cccagtattt atctataagt tcccttaaga gcctgacggc tctttttgct ttagctggat
    22201 taccgcttag ggtataaaca tctctgaata ttatctcaag attacagcct tttgctgctt
    22261 ttagtgtatt aagcatgtac ttactatatt catcatcatc aaaatcatac cccacaccaa
    22321 gaaaatgagg agaaggtttt cttgaataaa taatattagt gcctctgagt ctctcaccca
    22381 taaactgctc atcacaccat ggtgatattg atacctttct catgttagaa agattcttta
    22441 agcatacatc ccatattgga tgaacagctt cgcagcagcc ataataaacc ataccatatt
    22501 ctttagcaat tctttctata tacggataaa cgaattcttc atacatggaa ggtgatatgc
    22561 ctgtggattc ctgagagttg acattaaccc acatatcctt aagccttact ttcccgtctt
    22621 caggcatttt caattcatga gtaaagcctc tgcttcccgc tcctgcataa tagttaccgt
    22681 tattcatggt tagaagacca ttttcttcaa gccagtgctg atatgaaagc atattactag
    22741 taatgtagtc cataagctta tgagacattt caggagtctc aatcatagct atcatccatc
    22801 tttccatact catcagctgt acggccttta gagaaatcat atttccccat ctgtggaatc
    22861 cgctttccat ctcgactttt aaaatatctc ctaatatatc cctagcagct tcaattctgt
    22921 taaaagagtt ttctttatta acagtataag taaatggttt caatatctca aaatcagttt
    22981 ctaaatcttt gatggggtac tgatattcaa atcctatctc tcttccaaaa gagtcctttg
    23041 attttacaac aggtatatca aatccaaact ctttatattc aatatcccat ccgaatgtta
    23101 aaaaatccgg acaaacttca tcatcatcta aaagttcatg aacctgaatg ttattctgaa
    23161 tagcactttc gatattgcgg gcaaaatccg attgcgtctt taatgggatt ctgacatcgt
    23221 ctctaaatga tgccagttca atgataatca taggtttgcc ttttttattt tcattgtgat
    23281 cataccacct ctttatgcgt tcctgcatga caggaagctt tgatatctcc aattgttttt
    23341 tggcgtaatc cctaagatat tctctttccc atttctcaat tttatagttc atattacccc
    23401 ctgattctta agatatataa tagctaataa aataacatta atcaatgata tcagacaatt
    23461 taaccaaagc gcctcataag aagcgctttg gcattatctt ttaacactat atataataat
    23521 tttgctaatc cagcttgatt catataaata ttattaaact tctggtatgt ttatctcaat
    23581 ctctcttcct aactctgctg atttgtttat gccatcaata atagcctgat tgtaaagtat
    23641 ctgacttgaa ggtattggag attctccatc ttcaattatt gcgtccaaaa atgatcttat
    23701 cttcatatca aagagatttc cttttttctc aattatagga ataactgttt caacctgttt
    23761 tccgcaaact tcatgataca acttcatagc accgccgact gatccgttcc agcattctgt
    23821 tgatggaatc cttagtcctg cttttgttcc aagaatcaaa gtatctcctg ctgtatccat
    23881 attcatggcc catgaaattc tgaaatcaag tacaattccg ccttcaagcc ttacaaatgc
    23941 agcagcaaaa tcatctacac cgaaagcact tgcatattca ggatgatttg gataatattc
    24001 tggtcttttc ccgaagaaat ctgacttata gcctgagact gtaagaggct ttggatatcc
    24061 tattgcattt aaaaccatgt ccagtgagta acagcctata tcacccaaag ctcctattcc
    24121 acctgtatcc ttagctatga atgatgtacc aaacggagtc ggtatacctc ttcttctgcc
    24181 tccgcctgtc tgtatgtagt aaacatctcc aagctctccg ctttgaacaa ttttctttat
    24241 catcttcatg ttttcatcaa aacgcggttg gaaccctatt gataatatct tattcgatgc
    24301 tttttcagcc ttcattatgg caacagcttc atcaagagtc acacacatcg gtttttcaag
    24361 taatacatga actccttttt ccaaggcata tattgcacac tcagcatgtg tacagttata
    24421 tgtacatatg ctcactgcat ccagttcctc attatctaaa agctctttat gactcttgta
    24481 cagacgtacg tcttccaatc ccattttctt gcagaaagct tcagctttcc catctattaa
    24541 gtctgcacat gctacaattt tagcatcatc acatttctga tatgctgtaa catgaggctc
    24601 tgctatccag ccagtaccaa ttataccgat ttttaatact ctgtctgtgc tttccttttt
    24661 cttttcatca tgaatctgtt taaacttgtt gagaatatcc tgtgacattt cttcctcctg
    24721 tttaccatat aaggttattt aaatatttta tacttaattt tgcgcattcc aaagccgttc
    24781 tacctttttg aggcttatcc tgttcaacaa ccatccattg cgctcctgct ttaacagaag
    24841 cttctaatat agctggtata tcctgcatac ccatacctaa aggcctgaaa ctaaatgctt
    24901 cctggttatc cttttttgat tctttatcat ctgagttaac acctataagt ccatacattt
    24961 ttacatcttt agccatatcc tgtttataga aatcctttag gtgaacaaca ggtgatctgt
    25021 tagtatattt ttcaatatat tcagaaggat caactcccgc tactttgacc cagcaggtat
    25081 ctatttccgt ttttaaaagg tctttcgaaa tagtatcata aataacatcc aaaccaaact
    25141 ttccattaac tttcacaaac tcaaaatcat ggttatggta tagaagctgt aatccataat
    25201 cttttgcttt cagcccaagt tcttctatct gcttcaaagc tttatcaaat aaaggtttac
    25261 ctggtcttct ttcttctgga agatacggca ctgctatata tttacatcct atgaatgaat
    25321 atgttttaaa tacttcatct gtactttcta acatttcatc aagagctaca tgagcagaaa
    25381 cagggataag tcctgtctca tcaagcaggg attttacata ttccggttct tggttatgca
    25441 aagatgcaaa ttcaaccccg tcatatccgt attcttttat tttctttatt acattctgaa
    25501 aatctgaatc caaatcattt ctgactgaat ataactgtat agctactggt aaagacatcg
    25561 tgacctcctt aacgtatgta ttattgtatc attacagact gcctgtaaca acatatatac
    25621 tgctaaggaa tattattttt ttcacgactg attatatctt aaaaatgacc ttcatgttta
    25681 ttttacaaaa tagcagatat ccaaaatcac attccccaaa tcatctggtg ttgtaaagcg
    25741 aggacaacca tatctctcaa aaaaccagta tgcacctttt tcatcaggta taatctcata
    25801 tccttcttct tccagccttt taccgcattc atcttcatgc atatacagct cgttctcttt
    25861 gccgtaaatc cagcatactc caagattgct ttctggaaaa tcaacatatg agaagccttc
    25921 tttgactttg gtgtctttcg gcataaaaat ccctatccag tattcaaatg cgtcattatg
    25981 tttacaacgc ataagaccca catatgaatc tgagtcctca tattcatttt taattgttga
    26041 cagcgcttct acctcatcaa accatccgtt ttgaaaccat tcgctccact tagctccgaa
    26101 tgtgccgttc tgcctgtcac tatttgagta ttttttacct ataaaacgaa aggctggaat
    26161 agtctgtctg tatactttta ctatctgtgc catatttttc tttttccttt cgcagatatc
    26221 tattttacat aaaagcaaag atcaaatact atatttccgt cttcatccgg caccatgaaa
    26281 catggacaac catatctttg aaaaaaacag catggacctt cttcacattc aacaacatca
    26341 aatccggttt ctcttagttt ttcaatatat gtatcttcat cgttatatag ttcatcttta
    26401 ttgccgaata taacgcatac accaagtttg ttatgcttat agtcaatgta atcaaatcct
    26461 tttggtactg gactttcctg agaaaccagc attccaatcc agtactccgg ggtgccagtg
    26521 tttttatgac atacaagacc cacataagcg catccgtctt catattcctt gtgaggtatt
    26581 aagttgtctt ccaatatgtc gaaccatcct ctattaaacc actcagacca ttttgcatga
    26641 aaagatccat ttaccttgtc ttcttcggta tatttctttc ctataaaccg aagcgcagga
    26701 acttgttgaa tataggtttt tattatttca gccattttgc tacccccctt tcctcaagta
    26761 aattttccat ttatagttat atcagtatct ttattttatc agataacgca tttaatgtaa
    26821 attaaataca aaaaagtaat ttggaagtac tctttttatg tcctgccaaa tttgtattag
    26881 tggtaaaata tactatagaa ggagtgtaag taattatgcg tttaatgaca tacaatatct
    26941 gctcaggaat gagctatggt gaagacagaa gaagagattt aaatcaatgt gctgaagtta
    27001 ttaaaaaata caatccggac atattagggc ttaatgaagt acattacaat ataggatttt
    27061 caggtttcgc taaacaggcg gaggaattaa ataagatact taaatatgaa tatatgtatt
    27121 atggaaaagc aggtgcaatt aaagaaggat atcagggaaa tgctcttttt tccagatttc
    27181 caataaaaac agctaaaact gttcttgttg atcagccttc catatgtgat gaagacacat
    27241 attatgaaca gcgttctttc atagattcca ttatagaagc aaaaaatgat ttaagagtta
    27301 ttgtaaccca tttcggacta gcaaaagggg aaagaaaaaa tgaactaaac cttcttcttc
    27361 caatctttag aaaccataca gataaattgg ttttcatggg tgatctgaat gttgaaccag
    27421 atgatgttca gcttaagata atatcatctg cacttaagga tactgctgat ataacaaaag
    27481 acaatttata tacctggcct tctgatatcc cagttaaaaa aattgactat atattcgttt
    27541 cacaagacat taagactgat tcggttaatg tacccacaac tatggcatct gaccatctgc
    27601 catatatagc tgatgtatct ttttagctat cgaaaagttt tataaagttg tcatataaaa
    27661 tcatctggtt ttcttcattt gtcagtgaaa gactcaaaaa tctttccaac tccttctcat
    27721 ggtcccacat aggaaaatca gtcccaaaga gaaacttatc tacaccaaat gttctgatta
    27781 tatcagttgc ctcatcaggt ttcagagcat aaagacttga tgatgtgtca aaatacaaat
    27841 tatcaatatc cttaaatgat ttgtatgctt cttcccatcg tttatatccc ccaaaatgtg
    27901 ccgctataca tttcaaatcg ggaaacttat tcaatatgtt tacaaacctg taaggtcttg
    27961 tgtattcata tctgtcatcc cctgcatgta aaagacatac tgtacccatt ttttcacact
    28021 tcttatatgt cttaaaaaac cttgagtcat ccaagtctgc cttttgaaaa tcatgatgca
    28081 tttttatgcc tttcaatccc ttattaactg ttctctcaat ttcctcttca acaccaataa
    28141 aatcctgatg cattgcagct aaaccaataa actgcttatg ctttttacat tcatctataa
    28201 taaagtcgtt tattacagtt acctgagcag ctgaagtagc tacagaacaa accagatatt
    28261 tttcggcatt tatctttttg ccgttttcaa taagcgagga agatgttcct gtatagagca
    28321 taggaagacc ataaaaatgt cctatagctt cagatgcctt ttttgcaatc ttctcagggt
    28381 atatatgtga atgtgaatca ataactttaa aattgctatt caaaattgcc ttcctttttt
    28441 atcaaacaat gcttagtatt ttacacctat taatataaac ttcaagagaa ttattaatct
    28501 gcctgtgctg agaattttga atttatggtt tcatataact gctcagtaag ttccaatgtt
    28561 tcctggttta gcacaataaa tccacccttg tgttctatta gtacgtattc ttttacatct
    28621 ttataggcaa acagcttgta tcttccatat agttcatttt taaatgtgcc gctatacatc
    28681 cttataaggt ctgcacccat aactctgctt ccgacactta aactggtaag tttttccact
    28741 cgttcaatat ccacaaaatc cactgttact ctgtccagac tggactttat atttacagac
    28801 tggtcatcca cagtaacatt tactttactg aatataagaa gtgctgctat cgccaataca
    28861 agtataatcg ttattatcat tccagattta ttcattctga acttactttt cttttccata
    28921 aaccactcct aagtaaaatt agtataacac aaatgtttaa aaagatgtca tctcatcaaa
    28981 aagcttctta gcatacatat cagtcataga ggaaatgtaa tctatcacgg ctaatatata
    29041 atccgtttca ttatccaaat tatataaaac agtatttaaa tatactaatg gcctgtccgg
    29101 tatgtcagaa tatttcttaa gtctttcgga aaaagatgat gcaagttccg gataaattga
    29161 cgaatattct gcttttatat atgcaagcgt atcatttttt ctgtaaaaag acatcaattt
    29221 atcaaacaag gaatgaatga tgagctttgc ataatcctca tacattttca aacgtgcatt
    29281 tttatatata tgctcataat taaaagtttt gacttttttc aacatcataa atccttcatc
    29341 agaaaaacat ataccatctt tagcagaact gttcatacac aaatctgtta caaggctgtt
    29401 tataatcgta gtattattca ttgtctcttc agccttttta tactgattta aaatctcatc
    29461 aagactctta atatcatcac tatcaagcat cttcaatctt agcgcatctt caatatctct
    29521 tcctaaatat gctataatat ctgatgcttt cacaacacat ccttcatatg tataaggtga
    29581 aacagatgaa gcattattta ttgattctag ttcaataaag tcttctcttg gcatcagctg
    29641 cttcttttca atttctccgc agtgagtgac aatgccgtct ctgactgcat atgtaaggtt
    29701 cagattgtct tcctgaccgg aagcattttg aagcgtttca cagtaatcaa taaaccgtaa
    29761 actgtttttt tcatgccaga aatctgtctt cagatattgt ttggatattt cgtttattat
    29821 cctctcccca ctgtgaccaa aaggagcatg tcctaaatca tgccctgctg caatagccat
    29881 tataagctct tcatttaatc ttaaatattt agcaatacca aaactaatag aagaaacatt
    29941 gttgacatgt tccatccttg tacacacatg gtcattcctt gttgcaaaaa atacctgtgt
    30001 tttctgcttg agtctgcgaa atgccctgct gttaattatc cttgtgtagt ctctgtaaaa
    30061 ttctgaacgg atatcatttt ccctaacaga aatttctttt tttctggaaa tcatgttttc
    30121 ccatttagga ttctcttttt ttgctgatac atcagttaat ttattcattt aaaaccacca
    30181 gctcggaagc catcttagaa gctcgatata ctcgacgctt acttttaccc cggtcatcgc
    30241 tggatagaac gctgcaaaaa ccagggcgct caataggaca tatagattaa cgtatttctt
    30301 aattttacta tttccataca tgaaatatga agccatatag acaattgcca gtattaaaaa
    30361 cggaacacat gcgaaataat gatacaaaaa ggttatgctc cttggagcaa cagcccatgg
    30421 tagcaggatt gaaagataac ctgcaaaaat aaacaatcct cttttatctc tttttttaat
    30481 ggatactatt gctgttccta ttaatgcagc gacacctgtc caccatatta caggatttcc
    30541 caacgacaca attgttcccc attgaccttc agcctttatc ggagctgaat aatagtaaac
    30601 cggtctgtac attataggcc atgaatacca gctggattta aaaggatgat cctgtgttac
    30661 tccttcatga taactgaaca tatgcttctg gcttgctatg atattatcag ccatttcagc
    30721 tatagtgaac ttctggccat caatagctgc tgactgtatt tgaaatatgg gggtgtaaga
    30781 tactgaatat attacagctg gtattgctat gaagaagaaa atgcatatta tacaagtaag
    30841 caaagtattc ctggctgcaa gcgcttttcc accatgcact atccttccat aaccaaatga
    30901 tttccattcc atatatattt taacaaagaa aaggaatgct atacctgctc ctgcataaaa
    30961 tcctatccat ttaactgata ttgcacatcc gaaaaataaa ccggaaaaga ataagggaac
    31021 aagcgtcttt atataactgg tctttctcaa atccatatcc agataactgg ccatgaaata
    31081 aaaagtaagc attataaaca agacaagata gctgtctacc gtcccaagtc ttgtctgcac
    31141 gaaatgcata cagtcaaaac ataataaagc ggcagctacc attgcaagat aatggttttt
    31201 gaaaagcttt ttacccagag catacattag tggtatcata agtgctccag ctatcgctcc
    31261 catcgctctg taaccaaaag gattaactcc gaatatctta tatgaccaca ttataatgat
    31321 tttacctaaa tggggatgtg tgttctcata tatggtttca ttacctataa gaaattcata
    31381 agctgttctt ggaaaatata tctcatcaaa atatgtactg ttcatatatg aaggctcata
    31441 tacaaataaa tcctgttcat caaaaagatt cgttgctgat tcatccagat acggatttgt
    31501 aggagtcagt gatttaatag cgattggttc ctgctctcct tgtgcaaaaa aacacacctc
    31561 cattatctcg cctcttgtac ctcgtatgaa attctcttca ggatcctggt atatgcctga
    31621 aacgaccttg attgaatcgg tagttatagg ttcctcaaga tttaagtaaa gccatttcag
    31681 aaatttgctg gctccttttt caagtgttgc aaaatattca tatcttccag attggtcttt
    31741 ataatataaa tcaagagcag taacgttgta ccatgtatct ccaagtcctg cgagaaatgt
    31801 tattctttct attgtttctt cttgttcaaa ttcaacaata accggtgaac ctgccttctg
    31861 cggagtccaa ccatttaccg gacctttagt attaccaagg ttaacaagag ccaaaaccag
    31921 atatagcaga gtcagtatta atatagcaag tttttctctt gtcgcttgtt tgttcttgtt
    31981 ttttgttttc tttttcatat atgggttatt cggaatcttt tttggtccga agaagccttc
    32041 taactttttt tctgccaaga taccctcttt gcgtcatgct tgtatttgta tatagattat
    32101 atccttatac aagagcaaat aaaaggctaa aaactgtttt tttgaacttc catgattgtt
    32161 acaatacact taggattagc atgttccttc attttttgag catcttgtac agaccctcct
    32221 ataatcccat aatgcattgg aacaaatact cttgatttaa tatagaatgt ggcttttgct
    32281 gcttcctttg ggttcattac atatttatca tctggaaata ctggaataag cgctacatca
    32341 gtttctattt ttttcatctc ttcaataaga tcagtatccc ctgcatgata aattgttata
    32401 ccatccatct taactaaaaa acctaaccag ccgttttcct ttggatgatt aggtttattt
    32461 acattgtatg caggaagagc ttctatttcc agtccattta tcgcaaactt gtcataaggt
    32521 ttaacgataa tcatattttc aagtccaaga atcttggcac tttctgcttc ggttactatt
    32581 tttgtatctt ctttcagaat tttgttcaca tcatctttcg agcaatggtc gtagtgttta
    32641 tgcgatataa atatgatgtc agcctttttc cctatatcta ttttgaaagg atcaaaataa
    32701 attgttttgc tcccatctac tctgaatgat gcatgtccaa gccattttat cttatcagtc
    32761 agcatctttt cttgcctcca cttttacttt ttcaaccctc atgttgttta cttcaagtaa
    32821 agtcattata tgcccgtcaa aatcaaaact gtctccctgt ttaggaagat gacccagcat
    32881 ctcagtagca aaacctccta ctgtagtgta ttctgattca tactcttcct cttcgtatcc
    32941 tatcatatca aaaaaatcat ctaaaagcat atctcctgca acaatatatg tgccgaattc
    33001 cgtttcaatc ttttcctctt ccagatcgtc tgtctcatcc catatatctc cgactaactg
    33061 ctcaagaacg tcttccaaag tgactatacc catagtaccg ccaaactcat ccagcactat
    33121 tgccatatga gctcttttta tcttgaactc ttccatgact gcagatatac tcatagcttt
    33181 gtaaatatac aaaggttctt tcatcattgt tttgatatca attaatgcac ctttttttac
    33241 tgctgccttt aaaagcttct ttgtgcttaa tatacctact atattatcca tattaccctg
    33301 atatactgga actcttgaat attcttcgtt aagaactttt tgtataatct cttttggatc
    33361 ttcatcaata tctatagcaa atacatcaac tctcggagta agaatttcaa atgcaagatc
    33421 atcagaaaat tctattgctg atcttataag atcactttct ttttccgtaa agccaccttc
    33481 ttcttcaatt gtttccacta ttgataaaag ctcatcttct gtcatcgtag gttccgttgt
    33541 tcttggtttc caaagctttg atattttctg agtcattttc gtaacagtaa aaacaacagg
    33601 cttaaagata aatatgaata tctttagaaa tccggtcatt ttgacagcaa atctgtttgg
    33661 gttatcatta gcgacaagtt ttggggttat ctctcccaat attaatataa caactgtagt
    33721 tatagctacg gtgatagctt ttatcggcaa ggcagaatta gttctttcca atagccttaa
    33781 tgccaataac gtcgtaaggg atgatacggc aatattcaca agattgttac ctactaaaac
    33841 agtatacaaa actgaagaaa agtctcttga cagctcatat gtctttttct tcttatcatc
    33901 tcctgcttca gattccagtc ttaatctgcc aatattcaaa gaagtaaaag ctatctcagt
    33961 tcctgagaag aagaaggata atattactaa aactaatatt ccgatatata tgagtatatc
    34021 cattatccct tcacctcagt ttcattatcc ttactttttt ctttcttttt tgattctttc
    34081 tctttgttat tttcattatt atcttctgtt tcttcctgtt caattacctt tatacttaac
    34141 ttctcaatac gcataccatc aagctccttt attgttacca ataaaccaaa gtcctcgaat
    34201 gaatctttct catttgggac attctgaaga tgctccattg tccatgcaga aactgttttg
    34261 tgttctatta tgtcatcgtt atatggtatg tcaagtatat caagaagctc atccatgcgc
    34321 atctcaccag ctatctcaag agaaccgtct tcaacatatg catattcttc ttcaactata
    34381 tcatcttcat caaaaatatc tccgactagt tcttccataa tatcttccat ggttattagt
    34441 ccagctgttc cgccgtaatc gtctgtaaca attaccatgt gcgttctgtt agcactcatc
    34501 atctcaaaca aagtatttac taacatgctc tggtgtacga atataggcct gtccataatt
    34561 ccccttatat cgacagcttc attattcagc atacccttta gatattttct tgtctgtaat
    34621 acgcctatta cgttatcaat cgtattttca taaatcggca ccctggaata tttttccttg
    34681 attatggtac cgtctatctt ctcttttcgc cagtctatat ctacagcgac gatatctact
    34741 ctaggagtca gaatatcaga tgcggtagta tccaaaaaat caattgctga ctgaataagc
    34801 tcactctggt cagattgtaa tactccttct tcttcaatgg tctctatcat actttgcagt
    34861 tcatcttcag tcattgtagg ttcttttata ttggttccca aaatcttttt cataaacctg
    34921 cccacagcag acaatatata tgataaagga gtcagcacaa ttgagataaa ctttaaaaca
    34981 ggagcaaatg caatcgcata tgcatcggca ttatcttttg ctatgctttt tggtattact
    35041 tcactgaaag taaatataag aagagtaaga acaacagttg aaaccaatgt tccaatttct
    35101 ccaagaagcc ttactgcaag tactgtggca atagatgcag cggaaatatg tataatattc
    35161 agaagtataa gaatagaggt aatcgattta tcggattgtt cgattatctt cagcactcgt
    35221 gttgatttta tatcgccgtt ttcagaatta tactttagtc ggatcttatt acaagatgaa
    35281 tatgccgttt ccatacccgc tagtagaccg cccgccacga taaggacgat cagggtgata
    35341 cttcggtata tactgtcact atccattaag gattttcaca ctccattact caaaatatat
    35401 cgatagttta aggcatattg ctattattgt caagcgatgc tgtttttttc gttaccaaat
    35461 ctactcattt tgtagtattc tttattataa tgat
//
LOCUS       DAOSXM010000014        33508 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2111
            NODE_842_length_33508_cov_11.486533, whole genome shotgun sequence.
ACCESSION   DAOSXM010000014 DAOSXM010000000
VERSION     DAOSXM010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864178
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 33508)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 33508)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:24:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,261
            CDSs (total)                      :: 2,229
            Genes (coding)                    :: 2,218
            CDSs (with protein)               :: 2,218
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 3 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33508
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_842_length_33508_cov_11.486533"
                     /isolate="AMR_MDS_2111"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            192..836
                     /locus_tag="PLQ68_03510"
     CDS             192..836
                     /locus_tag="PLQ68_03510"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HXK71751.1"
                     /translation="MFTLKVEKRENDSKGRQLRRQGIIPAVLYGKHLEESVSLQLPVQ
                     EAQKFLRTNFIGSKVELEIGKKKHLAILKEATYTPGVNKLEHLSFQALKAGEKVKSVA
                     KVILSGKEKVKEGLVMQILDEISYIALPMDIVDRIEVNVEKLVLGDVITVADLEFVKN
                     KSVEVQNALDTVVVTVTMPKKHVEETEETETAADEVALVGDTAEDEKAKEEASE"
     gene            973..1791
                     /locus_tag="PLQ68_03515"
     CDS             973..1791
                     /locus_tag="PLQ68_03515"
                     /inference="COORDINATES: protein motif:HMM:NF024702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HXK71752.1"
                     /translation="MKYLKPIALIVLVIAAAVVLYVIMTKEIKADYVVEEGVLLSCET
                     NATSLSVPSFIEGQQITKIGNRAFSNNSSLLNVKLPDGVTHIMKEAFFNCDKLREIDL
                     SETVGFIEPEAFLNCPDLAKINVHFDNQILSSFNGVLFNSSYTYLKYFPTGYERVEFY
                     VPTGTMIIEEKAFAHCLLENVFLPDTVIRIDNYAFEDCRNMKSITLPPRLNSLGQGAF
                     IKCNKLTSITISDGVEIMDNVVGNDSFKQQYLTYGAGTYIKVSNDKWEFYKKAE"
     gene            2073..2732
                     /locus_tag="PLQ68_03520"
     CDS             2073..2732
                     /locus_tag="PLQ68_03520"
                     /inference="COORDINATES: protein motif:HMM:NF014375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HXK71753.1"
                     /translation="MEKILKEFKVFFDEENKEKAVKYIMEKLESKQIDVITLYSKILT
                     PLLNNLQCDLDDKKICIWKEHVKTGIVRTIVECAYPYVIEMRDSLNKKKKGTAAVLCP
                     PEEYHDLGARMVADFFTISGYNTIFVGSNTPYRDFYNAVDYIKPDIVAISVSNYYHLV
                     AAKRMVEDLKKTVNGKLKIVVGGYAFAGDEENKIKQVGADYFAKSYEDILNIDSEEVA
                     L"
     gene            2729..3895
                     /locus_tag="PLQ68_03525"
     CDS             2729..3895
                     /locus_tag="PLQ68_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010250637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXK71754.1"
                     /translation="MKLGLKIALRFLTSNKGQTILIILGIAIGVSVQIFIGSLIQGLQ
                     KGLVDKTIGNSSQITINSNLEDRYITDYDQLIDKISLSDDNITKISPVLDNQAFLSYE
                     DNSQSLLVRGFDMDKADGIYDISNRIVEGKLPTAENEILLGINLKDEYGISIDDEISI
                     LTVDRKSIKSTVVGFFDLKVSTLNSNWGITTLKNAQTMFENEEKISSIEMQVNEDSIF
                     ITDEIALNVLNTIGSDKYKAVNWKEQNESLLSGLEGQSISSLMIQVFVIISVVISIAS
                     ILAITVIQKSRQIGILKAMGIRNSTTSYIFLFEGLILGFFGALAGILLGVGLSFAFTK
                     FAINADGTPVVDLYISYSFIILSGFIAVAASILAALMPAIRSSKLNAIDIIRNN"
     gene            3907..4593
                     /locus_tag="PLQ68_03530"
     CDS             3907..4593
                     /locus_tag="PLQ68_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010250638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXK71755.1"
                     /translation="MKKILELRNVNKIYGTDIKTQVLYDINLAFDTGSFNSIIGQSGS
                     GKSTLLNIIGTLDTATSGQVLIDGNNITDMSANELADYRNKTIGFVFQFHYLLPEFTA
                     LENVLIPYNIQNKPVTKEVIDKAKERLKLVGLEKVMNNPSTKMSGGQQQRTAIARALM
                     NDPKMILADEPTGNLDSDSTETIYELLRSINEEYKTTFIVITHDRKIAEKADRIIEIK
                     DGKINLDIYK"
     gene            4606..5751
                     /locus_tag="PLQ68_03535"
     CDS             4606..5751
                     /locus_tag="PLQ68_03535"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HXK71756.1"
                     /translation="MNSYERVIAAFMKNKVDHVPIYCGSVSSSVASEVMGYTMYVGGG
                     IQQYRESLARFQGEQAHKEFVEKSRQDAYDWTDKMGLDYVRPSYWRYPYTPSKMLDEF
                     TFQYEREDNTHWIMRYFEKYELFDCIEDTKKQIDDVDDLEEEVIIQEEYNKRYMPKES
                     MHKEYLDAMEYFNYKKASYTGGIGLAIPNTRPIWYEAVILRPDLVERYLDALMERALM
                     NIPLIASIGAKIVNGGGDCACNRGLIYSNEIFRNLIVPRLKRISDKCHEYGIFHAFGS
                     DGYFWDVADDLYIDSGIDAHYEADCSCGMDIRSLRTKYPNVTVIGGIAASTLDKANEQ
                     EVEKEVMEAIDAAKELSGAIVGCSNLVSPTTPIRNFMLMMDLLHKYK"
     gene            complement(5759..8545)
                     /locus_tag="PLQ68_03540"
     CDS             complement(5759..8545)
                     /locus_tag="PLQ68_03540"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HXK71757.1"
                     /translation="MDSLEKKGMNSSLLNNRKIFYALLILVFIIGSFLTWKTQTNAIE
                     TQKQETIMLAKSASSLIPKSYLLTLTASELDLEKEEYKIIKQNLIRFVENNELIHFAY
                     IFMLKDDDIYFAVDSEPENSSDYSPPGEIYYEAEEWDYIPFIQKIPTVSEPFEDSWGK
                     WVSVLVPITDYENNEILGVFGLDYPVSTWYMNTLRNTIQVGIMTLCVLAIIIVLQMII
                     SMNFELKKEKQLIFETDNRLRDSMALYKAVFDQTTIGIAIADNFKYLVETQTGIPSIN
                     PAFEKILGRPKEQIADVTWSEITNPNDLGVDLEYFKQFKAGIIEGYEMDKRYIRPDGK
                     EVWVHMIISPLHFENPHNKYHLCIIEDITSRKIIEKNLKESENSKSILLDNLPGMIYR
                     CKFDKDWTMLFTSQGCKELTGYDSDSLINNAEVSYNDIIAPKYRQYIWDKWITVLNNK
                     QKFIYEYEIVTADGQTKWVYEQGQGIYDEFDNVIELEGLIIDISDRKNSEDRIRYVNE
                     HDALSGLYNQKSLVEHFNNDIENEENISKAVIIIHIKRYNLMITAYGYSYCESITLDL
                     VNNLSSLTDETHMLYQLSMDRFALYVKNFKNIKELTQVAEKIVNILSYNVALRSYGAW
                     VGLDIYDKSIPDIHTTLKNATMAANFVKGIEPFGYSIYNTQMEESIRRQEDVKDVIAS
                     ICNDSKREVFHLVYQPILDKKGDICFFEALARIDDDKLGSVSPSEFIPIAEKRHLIVD
                     LGRIILDEACKFIKLLENNGQKDISVSVNISAIQILRDDFLSDLRYIINKNRIPTHML
                     ILEITESVFAENLEFINSQLAKAQVMGIRSAIDDFGTGYSSLSRERELAADIIKIDKF
                     FIDQILVKDEKELITSDIIHLAHKLGQKTVSEGIETNLQKEYLKRNDCDYYQGFLFSK
                     PLSAKDAISFLKKD"
     gene            8901..9635
                     /locus_tag="PLQ68_03545"
     CDS             8901..9635
                     /locus_tag="PLQ68_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HXK71758.1"
                     /translation="MKLTVKKTAALADISIRTLHYYDEIGLLKPSDISTSGYRLYDDE
                     EINRLMQILFFKELGFSLKDISDMMSNPDYDKNEALKKHKQLLILKKEHISRLIMLTE
                     QAMEGKVMEKDAEKIQQYEKIKEQYANEAKQKWGKTDAYNQSLKKHDSYNSQDKIKMM
                     KESEEIFKSFAQSRNLDPKDEKVQNLVKRWQDYITKYSYDCTKQILSSLAVMYTEDER
                     FKANLDKYGEGTAKFMSEAIMIYCKD"
     gene            9819..11123
                     /locus_tag="PLQ68_03550"
     CDS             9819..11123
                     /locus_tag="PLQ68_03550"
                     /inference="COORDINATES: protein motif:HMM:NF014135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsB/NhaD family transporter"
                     /protein_id="HXK71759.1"
                     /translation="MSTAIDIGSVTCITMILAVLFKPQVKIRRIFIDTYWPIAMIGAF
                     LLVIFKSISFKEAVLGLTADTAINPIKILVLFLSMTILSIFLDEAGFFHYLANATLKK
                     AESSQFMLFTYLYLTVSVLTIFTSNDIIILTFTPFICYFTKNANISAIPYLVAQFVAA
                     NTWSLMLVIGNPTNIYLATSYNISFIEYSKVMLLPTLAAGITAFLVLWLIFKKQLKKE
                     ISPSIEKIKISDKPSLIIGLIHLGLCTLLLVLSSYINIEMWPVSLGFAVSLFVCIAVS
                     KLIQKKKLAVLVSCIKRAPWQLVPFVISMFVIVLALDKYAVTDDISGFLGTDNVIYKY
                     GLSSFITANLINNIPMSVLYSSIAENLPLSLVSRGVYGAIIGSNIGAFLTPVGALAGI
                     MWTSLLKKHDIKYTFADFIRYGIVISVPTILASLFVLDIVLK"
     gene            11186..12091
                     /gene="pfkB"
                     /locus_tag="PLQ68_03555"
     CDS             11186..12091
                     /gene="pfkB"
                     /locus_tag="PLQ68_03555"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003499999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HXK71760.1"
                     /translation="MIYTVTINPAVDYTIKIPELVLGKVNRSEKEEYFFGGKGVNVSI
                     VLHNLGIKSIALGFIAGFTGQFVRQGIYSLGIKNDFIDLPSGMTRINVKIKADKETDI
                     NAVGPAIPKDSIELLCKKIEKVKDNDFLVLAGAVPNNVDEQIYSDLLSHLKAKNVHVI
                     IDATRNYLINALKHKPFLVKPNRAELGQLFNKEIHTDADIVLYAKKLQEMGARNVLVS
                     IGDKGAVLVTENDDVICNDAPKGITKNTVGAGDSLTAGFIAGYMEKKDFRYALKYGLA
                     SGSATAFSDTLASKNEIEQILKTIE"
     gene            complement(12123..14111)
                     /locus_tag="PLQ68_03560"
     CDS             complement(12123..14111)
                     /locus_tag="PLQ68_03560"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HXK71761.1"
                     /translation="MKTIYVSVNGNDEFNGTKKSPLKTLNKAVLLSRETGIKDIFILP
                     GEYFGVSISLCKKDNNLKISANKGTVILYGGFPLSKWESVDGYLVSDFWQASKDNGHF
                     QKKPFRLLKINEKLAKRSRLPQTGVFKHLCEWNQDCLPACYGHWDPKPTITQSRSIKY
                     SEKDIEKGLDTDSAELLIYHEWSATLAGVIAKDETKNMFYLTNSTDMPAGAFADRNEM
                     ADKYVVFNTKEGLHDINQWYADFNKGLVWYKGNIDETINGVFPVFDNILSLSNAKNVI
                     IDGLDIQMSNNDLINPGYGARHLSAAVDITSCENITIKNCNLSYVSAWGIGIKKCSSN
                     ILLQDCTFHDTGAGAVNFFENHDGENVTIERCQCFDIGKIYPGAVGIYGGGQKNLISH
                     CEIHDAPYCAINEIGNYSVIEKNLIWNIKQQMQDGGAIYLIFENNIVLRNNVVLNNRE
                     DHVQAFSYYFDELCEDCIAKNNLSVNATVSSQNHIAYRCTNKNNIHIDNGLMKVRFVD
                     CYDLKYYNNVFIADEILFYGPSKDRRYTLPDDLSDIVRKTVSKFQDATGITEISNNVI
                     YSKLKPPAFRLQYSKEEPSVYMPKGNIYDDPGFNDIDNGDFSVTEDSNAYKAGYRGIN
                     FDDVGCTNRFHELFKKFWPLKPNYMYGWAEKKDQVDNT"
     gene            14259..16778
                     /locus_tag="PLQ68_03565"
     CDS             14259..16778
                     /locus_tag="PLQ68_03565"
                     /inference="COORDINATES: protein motif:HMM:NF012906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-mannosidase"
                     /protein_id="HXK71762.1"
                     /translation="MTKVCINKGWMLQQRPLNEKEWSDMDKWGKQYKCDVPCDVAMPL
                     IESGDMPDPAIADNCYKNKWVEDRSWWFQKSINTDMLPLWQDSDSVELVFLSLDHEAD
                     IFLNGIHIANHNSCHYEFRKNIKRYLKEGDNLITVRLTSGLETIPKEELFEVDNAICT
                     EEPRRNDRGDKRRAFLRKPQYVYGWDWSPRAVTVGIADDVYLEISKTARITDVYIYTE
                     SIENNCAVIKGEIEVENMNILSTLDAESIRIDISLNDEIKAFWKEKDVFLCSGLNYIP
                     FELNIEQPQLWWPNNSGKQPLYKVNCNMNIGKRHISFPLFDFGIRTIQLDTARISKDR
                     RDFKFKINGYDIFAKGANWIPSDLLYMKVDDDKLEKIIKQAADANMNILRIWGGAVYN
                     RKLFYTLCDKYGILVWQDFMFACSAYPDSLEWFMDLCEKEIDYQTKRLRNHACLALFC
                     GNNECHEIFSIKNFMNWDINPDYSKQYGLSISNNLAKKITHRNCPNIPCWNSSPYGGD
                     IPQSSKCGDVHYWNDAMMSPDMDNRITLQIYDKVSSAFVSEYGYIGPTCKKTIEQYHG
                     DCPIKKGSFVWNLHNNTMEKDTVDAGIKKHYTDKKLTLDEYITYAQMVQYSVLNYSLE
                     AFRFSLLCNGGIFWMFNDCWGEVGWTVLDYYLRRKASYYGVKRAFEPVKIIIRLVDNT
                     ITAVGINETDKDMGFTAKTGWITFDGIKNETDNKTFRIPKRSRQVIHMEKMQKKDPSE
                     WIFSIIPIATEISSAILNIADACIRKTKNDVDILKYENDNDDLHVYLKTKGFVHGIYF
                     EEDYNYSDNYFDMLPDETKKVIVYGAKNKRLIPKTVILEHF"
     gene            16881..19469
                     /locus_tag="PLQ68_03570"
     CDS             16881..19469
                     /locus_tag="PLQ68_03570"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HXK71763.1"
                     /translation="MNNKTKTTKDTKNTKRRKNKKNTAILIVVAVLLCIAISLLYAFM
                     KGWFEPKVTPTPSNSQSAEPSISVSPPPSETETETETETPTDSPIIVNPIPEGHIKID
                     KFDDLKGYFEYMLSTKTGIYGLSVLDVTNNKSFNIATSQQFDNSSVYKIAVVLYAYEQ
                     EVRQGMPLDTLVEYTADDYDINSEVVAIGNYSDTHTIQKLCEYILKSDDRAALNMLLR
                     VYGKSNIEAYMSSMGVSESMSSFSPNDMAIFFDKLKQKTDASIDGYSQIHEQMNSGIL
                     DGVSTASLFTKDEDILSMNGSVIVYAKNIFIVSMSAKDANMAEAENTFMDIYSTINTL
                     FTENEVIVEKSIYSHALVLDNIVESAKQTASRFKSYIMMPDKTVTSSYVSDYDISFPR
                     SDEYSDIGVTTYRGNNYRDSGFFGNINISEQKLEEIWSYEIGGMTGAGSIWPGVGWTG
                     QPCIVKWDKEMLCMMNVYDKFKNETELIEVIYATLDGNIYFLDLKTGEPTRDYIYIGV
                     PIKGTLSIDPRGYPLLYVGQGLEAAKTNDGEERYKAFGYRIFSLIDSTLLHEIRGYDT
                     FAPRPGWGAFDSSGILDIKNDTYYECGENGVIYIIKLNTIFNKTEKTVSVNPEITRYK
                     YIYESEYAYGIESSPSIYEKYMYFTDNTGMLICLDLESLRILWAYNTGDDTDASVALD
                     VTEEGVFLYTANEVDKRGKDDYSNLRKFNALTGELLWQVDVPVYYVDVINGGALASPI
                     IGKGPIKDLVIFNICKTLEDSIWSGRLIAIDKETGQIRWEYKSPYSWSSPVDIYTEDN
                     KAYIVYCEHNGKISLIDALTGTQINQVTLSLGRQYITIEATPAIYGDILVVGTYAQKI
                     YGVRIK"
     gene            19471..19707
                     /locus_tag="PLQ68_03575"
     CDS             19471..19707
                     /locus_tag="PLQ68_03575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK71764.1"
                     /translation="MTIKKRKNSTLVAIVISAAALLLSIANLILCYIYDLEKPLATII
                     VVCISAVFSLNVAYYVSLKKKMKREMERKDEKDK"
     gene            19691..20158
                     /locus_tag="PLQ68_03580"
     CDS             19691..20158
                     /locus_tag="PLQ68_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002478035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070191 - methionine-R-sulfoxide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HXK71765.1"
                     /translation="MKKTSKSDDAELMKQQLAELVKDEPFVIPNLANASAFIMDTYID
                     LNWAGFYLLHDDTLILGPFQGKPACIRIELGKGVCGQSAKLDKTLNIPNVHEFTGHIA
                     CDSASNSEIVIPIHCNNKVIGVLDMDSPIFSRFTEEDQKQLEDLVKILEEQVF"
     gene            complement(20175..21068)
                     /locus_tag="PLQ68_03585"
     CDS             complement(20175..21068)
                     /locus_tag="PLQ68_03585"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HXK71766.1"
                     /translation="MNNILSILNNFYDMNFTDISKIRDAGSVSYTVYSGSLRYFLRVI
                     KPSFIDTAFSGANIQAFLCSKGFPVPPIIYTKDNQPCIRTDNGLFILYEFIEGKDSNP
                     EQDAKAIGELIGRLHNTMKNYTGELKDCDRQFYIVRYIDILKRKQYPKTDEFVTYGNT
                     LWDSVKDLPRGYCHGDMYDGNIYKTSDRKFYILDFYTSCYGLPMYDIALICNKTHYFN
                     FDESGYLKSKEILAHVLPGYLKFSTLTKTEINAFYDLIALYHFALQATVIENNGLDCV
                     NNAFLDKQLDWLYRWKNQCER"
     gene            21403..24030
                     /locus_tag="PLQ68_03590"
     CDS             21403..24030
                     /locus_tag="PLQ68_03590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK71767.1"
                     /translation="MKKRFIVCFTLVFILMSVIGLTLLTGLAESGHDEGDSIKEASNI
                     AILSIDGVSKELQFNSVTPSGPNSFYVVNDNEVYIADQYDKKINYYKNKMLEKEILFP
                     DVNFITDFYIADNELFLLDDYFSGAYRINDVICADMDGNIKYKINIPNDSTFLEMKNA
                     NNYDRRCRVRRIYDTNSYITCEFSNSINYIYTDSEWIEYKDDDLQKNINTHMVTFNKW
                     SNSIIQSQGEIEYADVIKTNKDVLIVSVGAVIKEESSYSKMQCLYMINEKGVSWVQPV
                     KTKANSVPDKQFFISEDNQIYQMYYDENNNTFISKLNPGEYSIRYSASESDPKHESND
                     ISNNKITLASYSSITREQIENRATDFLNHTWTFNCYTNADDSILVGCTGVSVACITHQ
                     NYNILPVFLPQIKCTNSCNGHTVTGIPYCWTGQCEMRIPADAPYTHLQLYINDGTTAF
                     DEQISNGYYAGNIPIKGNLFDVRNHYWLFKTAGVDCSGFMQVCFKYNDSAKINCNWIK
                     NTTDDFDSISSLANAKAMDLLVNSGHVMIIDEVEWNSTYDQIIRVWVYECTTAGYDKV
                     IYNDYTPSEISYKNYEIRRYKGVTTCTHSWSDWAVVVSPTCISTGKAVSTCSICGEVD
                     TYPIPIIAHNYKPATCESPQTCWVCNATNGTALGHNFTIYQNTSYQPYSSTQHMVINQ
                     YKCSRCPELDDEIQYASHTYNIPAATCDTAKYCTVCGNVAQPAIGHSYSTATCVSAAT
                     CSNCGVTSGSKNPNNHSGGSHTEVSNPTCTVNGSQSLVCDGCGAILSTSTILAPGHSM
                     SAWSVITASTCSTPGTQSRYCTNSGCGYSESSSIPALGHSWSGWVYQYTYYDPDWQSN
                     VHVYRRTCTRCGAVEYDYI"
     gene            24061..25731
                     /locus_tag="PLQ68_03595"
     CDS             24061..25731
                     /locus_tag="PLQ68_03595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK71768.1"
                     /translation="MRKAISVLLILVSLLALSSCDEVQSGTNTPAPSLTENTTDTAVV
                     PSSTDMASETALPTETVVAPTPTATPFITPTPTPNPGPRNIELDFLNDRFDKVEALID
                     GSYVDVSDIWESFKQIMGKPVFRYFDASDLMTIQNKIRFIKDGREFMLSPLKRTSTSY
                     GGSDYGTVSYQWSNTILHSSYYIPNISLFYTILNMDYLEREGETGLVLKEYFSGDVFQ
                     KMYDSTVLEYDGSYIWTPKKAVPIYNIAKHMKNRMDLKPDELGLYTIVRSYTYIYSLE
                     TRISLDIYGKDESGNGVYANVKCGDDDIWMVLDCSILDFDNGLSVNDTYHVPSGAIPV
                     RDGEWFDGYEKIELVYGEAITDVTEFWYRNPDCVKICDWETYLSGPALPVEYEYRMTK
                     NGTVKTMSVSDMYDYFISEGDGLYYCDYLTSYKNAFIMTEEEAVIEDFINRSIYQKMF
                     LCAILDITKENEDYRNILMNQKACQFATSLAFRIKEHLNGKPDMEIVRRSESTCYYKG
                     EVISVIAWDIPGDGYNLGYVNILSGDTDIWLKIQNIQFLLGFLGVNDW"
     gene            25961..27088
                     /locus_tag="PLQ68_03600"
     CDS             25961..27088
                     /locus_tag="PLQ68_03600"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXK71769.1"
                     /translation="MFNNMKIADCHIHYSSNISVDEMAKIFDYCNTDIANIVVVPDKL
                     KLSAVQDALILKHKLQDRIYVFASLDASCYFINKKTLGKSMAKHALLMRKLGCDGIKM
                     IEGKPQIRKMLNVPSFDSPVWEPYFKFMEDTAFPILWHVNDPEEFWNKDKIPKWALEQ
                     DWLYDETFINNEKQYEEVLKVLRRHPNLKIIFAHFFFMSKQLDRLSSILDKYPNIYVD
                     LTPGIEMYINFSENKEKAVAFFEKYQDRIMYGTDIGTKGVVDGKSASLEEEKKRADLV
                     KRYISATEDFLVDKQYSYLMHDKDFILSPLALSKKIQSKIFAQNFINFIGHVPYAIDS
                     KKALWEVRRTMFKLLLMKIGDKTTKYDFKSLKKACEQLKRI"
     gene            27102..>27550
                     /locus_tag="PLQ68_03605"
     CDS             27102..>27550
                     /locus_tag="PLQ68_03605"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXK71770.1"
                     /translation="MQKIKRKHLPLYALSGFGPGLVNLMITAYLIDAMSTAGFLKNIE
                     NWTYANKTIVATALFSVFVLIAKVVDGIADVPLASLTDTLKTRWGKRRPAILIGYIPM
                     IISFILFCFPLETVENSMMNTVWFGILLILFFTSYTLTLVTYYGTYS"
     assembly_gap    27551..27650
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <27651..28226
                     /locus_tag="PLQ68_03610"
     CDS             <27651..28226
                     /locus_tag="PLQ68_03610"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXK71771.1"
                     /translation="LSGQNVLASGPMGLNYWQITIINTAAFAPVPLMLYFYRKIMKKK
                     GFRFAFQTAMGSFAVAMLMFSVAYIEWIPNEYIRLAIAATGATIGSYGLGAFFSAPYA
                     IPSQAAADELKATGKSHPSMYFAMQGLCTALVGALSTSIVWLNIKEITLPDNPVFGAH
                     LMPYIVMIACVVAIIAANFMPKEYNEMGKEK"
     gene            28223..29119
                     /locus_tag="PLQ68_03615"
     CDS             28223..29119
                     /locus_tag="PLQ68_03615"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HXK71772.1"
                     /translation="MSVDNSNSVKLYLSIDIGGSAIKYGVLDETFSFVYKSKMPSKIF
                     LGGKELLSRVMSIVADISKKYSIEGVAISTTGSVDSKNAKVLYADDVLEDYTGTDYKK
                     LIKQEFDLNAAACNDVNCLALSQMKFNKGKDFIVVAIGTGIGGAIVISDKLYEGYCYS
                     AGEFGLMHLRDGKNWETLCSMSALVKSSKEHKLLISNGKELFDMFDANNDVAYKIVDS
                     FYKDMASALANLIYAFSPQKIIIGGGISARGDSLINGVKKYLGIMMDEFYYEKTSIEC
                     AVYENDAAMIGALQHYLFMNHK"
     gene            complement(29095..30561)
                     /locus_tag="PLQ68_03620"
     CDS             complement(29095..30561)
                     /locus_tag="PLQ68_03620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK71773.1"
                     /translation="MQDTIGFEMDNTEKTDKILLLESYKNNVLRFIERCVPSDGVFDE
                     EAFKAAGGKNVRGSGSDKFIIPKEKRIVKTLTLKERAKNNYWAFSSTPGGVESIEGNH
                     SAIEALYLLGELDTLTDEDKANWADYINSFQDEATGYYLGPYIPDKSDPSWSDASVNT
                     HPWYHMHEHLIACLCPAIMLLGAKSKYKLSKGSMTGRYLDEDFLKDYLYNRDWNNYKF
                     DLNFRRHNPWWMGNEFWNAGCILWQIATWEEGTKEGDKARKLLDEIWYKWHDENIGIT
                     GFWYGDLKTDKEKIWHHVMETGFETLTPKTSDEYCWISSPIMGSAHQLWLYAYENHDI
                     PDELRKRQTDMILLLQRNDGLFGLRAPDDPLSYNDNTIDTDCMTLLAFNYNRIDYRRE
                     DIKKALKKAVLSIFKYKINDYGVLESRMEVGFGHNFNSVPTLSPANWGNMINMSFYFW
                     PLICAFNILDKSECPELSEFIDHDWPQPVSHYLWFMKR"
     gene            complement(30546..31124)
                     /locus_tag="PLQ68_03625"
     CDS             complement(30546..31124)
                     /locus_tag="PLQ68_03625"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HXK71774.1"
                     /translation="MDTYDTILKRRSIRKFTQKPIPRPLIDKMLNAARLSPTGMNMQP
                     LRFYVVTDKQLLDKIFPYTKYAGYMPDGECSPSLEEGPTAIALILNDLSIRRSDDVAA
                     GAAAMSMILTAQNEGVSSCWMMAIDKNNICNICGVDQKKLSLHSLISFGYPAMTSRAE
                     PMKYDKSVRYYFDEEGTLCVPKRHFEDVCKIL"
     gene            31210..>33508
                     /locus_tag="PLQ68_03630"
     CDS             31210..>33508
                     /locus_tag="PLQ68_03630"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF023636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH116 family glycosyl-hydrolase"
                     /protein_id="HXK71775.1"
                     /translation="MIYKGEKTKEISFPLGGIGSGSIGIAGNGSFMDWEIFNKPDKGS
                     TNGYTHIAVKAEDINNSLVSAKILNCDMDKEYLGRYSTSGGFGFGMLKQTMAGFPHFK
                     DGVFKGEFPIMELSLKDKTFPAKAKLTAFNPFIPLNEDDSSIPGAFFEVELKNDTDNP
                     IYYTVCFSVKNPYIKGINKYMPNSKAEMVFMKNADKPKEDIEYGDLCVSLVKQKDAET
                     SYQTNWFRGGWQDSIETFWNDFTKNTILPKREHSDAGKYDTCSVCIRKLVESKKSMKT
                     RLLLTWNVPNNYNYWNPYMKDDKHVTWKNYYATLFKDSCASAKYSIKNWNRLYKSTNL
                     FRKAIFSSTYPDYVKDAISSTLSVLKSPTVLRLENGEFYGWEGVDEKQGSCSGTCTHV
                     WNYAYALPFLFPKLERSIRDTDYKYNMDENGKMQFRMNLPLGRDRHQWRACVDGQMGG
                     VIKTYRDWKISGDTKWLESNWEAVKKSLAYAWSEKNEDGWDCDKDGVLEGRQHHTLDM
                     ELFGPSAWLEGYYLGALKAASEMAFALGEREQAWEYEKLYMSGRGWSFDNLYNGEYFI
                     QKINLKDKNLLEKYDSVDRYWNEDKQQIKYQIGQGCDIDQLCAQWHANICGLGEILNN
                     KRTKKALKAMYKYNFKKSMRDVVNPWRIYAVNDESGAVICDYPAHVEKPVLPVPYNTE
                     CMHGFEYMFAGLLISEGLIEEGLSIVKSVRDRYDGRKRNPWNEVECGSNYARSMAAYA
                     LVPILSGMTFDMTTYTLGFDPKTSRE"
BASE COUNT        11302 a         5265 c         6435 g        10406 t
ORIGIN      
        1 aatttgcctt tatgattttc ttgtggtaaa atagcgaata ggccgcaaat gcaatttatg
       61 cagattttcg gtgtaaatat gcttttttac cgttgtgatt cactctttta aagaatgttt
      121 tgcaaataaa gatatagaca catggtaatc agacggtata gagtaaaaaa ctaaccctga
      181 aaggagatat catgttcaca ttaaaagtag aaaaacgtga aaatgactca aaaggaagac
      241 aacttagaag gcagggaatt atacctgcag tactttatgg caagcatctt gaagaatctg
      301 tttcattaca gctgcctgta caagaagctc agaaatttct tcgtactaat tttatcggtt
      361 caaaagttga attggaaatt ggcaagaaga aacatttagc catattgaaa gaagctacat
      421 acaccccagg agttaacaaa ctggagcatc ttagttttca ggctttgaaa gctggtgaaa
      481 aagttaagag cgtggcaaaa gtcattttgt cgggtaaaga gaaggtcaaa gaagggcttg
      541 ttatgcagat tctggatgaa atttcatata tcgcattgcc tatggatata gttgaccgta
      601 tcgaagtaaa tgttgagaaa ctcgtattag gagatgttat aactgttgca gatcttgaat
      661 ttgttaagaa taagagtgtt gaagtgcaaa atgctcttga tactgttgtt gttacagtta
      721 caatgcctaa gaaacacgta gaagaaacag aagaaacaga aactgcagca gatgaagttg
      781 ctttagtagg tgatacagct gaagacgaaa aagcaaaaga agaagcttca gaataagtct
      841 gacagtatga ggacacacta aaagaataac taaacaagga taagaccatc aatggcttat
      901 cctgtttttt atctattaaa aacaaataaa tgtggtataa tcgtaaagac ttaagaaaag
      961 atgaggtcag aaatgaaata tcttaaaccc attgcattaa tagttttagt tatagcagca
     1021 gccgttgtgt tatatgtaat tatgactaag gaaataaaag cagattatgt tgtagaagag
     1081 ggtgtcttgt tatcctgtga gacaaatgca accagtctta gtgttccttc gtttattgaa
     1141 ggacagcaaa ttactaaaat tggcaacaga gctttttcca ataactcttc tttgcttaat
     1201 gtaaagcttc ctgatggcgt aacacatatt atgaaagaag cattctttaa ctgtgataaa
     1261 ttaagagaaa tagatttatc agaaactgta ggatttattg aaccagaagc attcctgaac
     1321 tgccctgatc ttgcgaaaat aaatgttcat tttgataacc agatactaag ttcctttaac
     1381 ggagtgttat tcaattcgag ttatacatat cttaaatatt ttcccacagg ttatgaacga
     1441 gtggagttct atgtgcctac tggaacaatg attattgaag aaaaagcttt tgcccattgt
     1501 ttactggaaa atgtcttttt acctgataca gtaataagaa ttgataatta tgcttttgaa
     1561 gattgtagaa atatgaagag tattacatta ccgccaaggc ttaattcctt aggacaagga
     1621 gcttttataa aatgcaacaa gcttaccagc ataactatct cagatggtgt tgaaataatg
     1681 gataatgtag tagggaatga ctcttttaaa cagcagtatt taacatatgg tgcgggtact
     1741 tatattaagg taagtaatga caagtgggag ttttacaaaa aagccgaata gaacaattta
     1801 ctaattaagt gtagaagccg aatagaacac tttattaatt aagtgtagaa ttggtaatta
     1861 taaaaataaa tttgtataaa ctatataaac tgacaggact atatatccta tcagtttttt
     1921 ggtgattaag ttaacgatat tttatattaa ttaaggttta atatgtttat tgatatatgt
     1981 aagagtttta agtactattt cctttattta attgatataa taatataagt tagaaatagg
     2041 aaatgaatat aaatgaaaaa aaggagaaaa gcatggaaaa aatcctgaaa gagttcaaag
     2101 tgtttttcga tgaagaaaac aaagaaaaag ccgtaaaata tattatggaa aaacttgaat
     2161 ccaagcagat tgatgttata acgctgtaca gcaagatact tactcccttg ttgaacaatc
     2221 ttcaatgcga tcttgatgat aaaaaaatat gtatatggaa agagcatgta aaaaccggaa
     2281 ttgtcagaac aatagtcgag tgcgcatatc catatgtgat tgagatgaga gatagtctta
     2341 ataaaaagaa aaaaggcaca gcagctgttt tatgcccacc agaagaatat catgatcttg
     2401 gagctcgtat ggtagctgat ttctttacta ttagcgggta taacacgata tttgtaggaa
     2461 gcaacacgcc atatcgtgat ttctataatg cagttgatta cataaaacct gatatagttg
     2521 ctattagcgt aagtaactac tatcatcttg ttgctgcaaa aagaatggta gaggacctta
     2581 agaaaactgt aaatggaaag ttgaaaatag ttgtcggggg atatgcattt gcaggagatg
     2641 aagaaaataa gattaagcag gtaggagcag attactttgc taaaagttat gaggatatcc
     2701 tgaatataga cagtgaagag gtggctttat gaaattaggg ttaaagatag cattaaggtt
     2761 cctaacatca aataaaggac aaaccatact cataatttta ggaatagcaa taggagtatc
     2821 ggttcagatt tttatcggct ctttgataca gggtttacaa aaaggattgg tagataagac
     2881 gataggcaat tcatcgcaga tcactattaa ttctaattta gaagataggt atattactga
     2941 ttatgatcaa ctgatagata aaatatcctt atcagatgac aatattacta agatatctcc
     3001 ggttttagat aatcaggcat ttttatcata tgaggataat tcccagtctt tattagtaag
     3061 agggtttgat atggataaag cagatggaat atatgatata tctaatcgaa ttgttgaagg
     3121 aaaactgcca acagctgaaa atgaaatatt gcttggtatt aatcttaaag acgaatatgg
     3181 gataagtata gatgatgaaa tcagcattct tactgttgac agaaaatcta ttaagagtac
     3241 agttgtagga ttttttgatt tgaaagtatc tacactcaat agcaactggg gaataacgac
     3301 acttaaaaat gcacagacaa tgtttgaaaa tgaagaaaag atatcatcta ttgaaatgca
     3361 ggttaatgag gattcaatat ttataacaga tgaaattgct ctaaatgttt taaacactat
     3421 cggaagtgac aaatacaaag cagtaaactg gaaagagcag aatgaatctt tgttaagcgg
     3481 gctggagggt cagagtattt ccagtttaat gatacaggta ttcgtaataa tatcggttgt
     3541 tataagtata gcaagcatac tggcaattac agtaatacag aaatccagac agattggaat
     3601 tttaaaagca atgggaataa gaaattccac tacaagctac atattcctgt ttgaagggct
     3661 catacttgga ttcttcgggg ctttagcggg aatattatta ggtgtaggat tgtcttttgc
     3721 ttttactaaa tttgctatca atgctgatgg aacaccggta gttgatttgt atatcagtta
     3781 ctcatttatc atcctatctg gatttatagc agtagcagca tctatactgg cagcgttgat
     3841 gcctgcaata cgttcatcaa aactcaatgc aatagatata attagaaata actaatggag
     3901 aacattatga aaaagatact tgaattaaga aatgtaaata aaatatatgg gacagatata
     3961 aagactcagg tactgtatga cataaattta gcttttgata ctggttcatt caattctatc
     4021 ataggacagt cgggaagcgg gaaaagcact ttgttgaata ttataggaac tctagacaca
     4081 gctacatcag gacaagtgct gattgacggt aacaacataa cagatatgtc tgcaaatgaa
     4141 ctggctgact acagaaataa gacgatagga tttgtgtttc aatttcacta tcttcttcct
     4201 gaattcacag ctctggaaaa tgttcttata ccatacaata ttcagaacaa gcctgtaact
     4261 aaagaagtca ttgataaggc taaagaaaga ctgaagcttg ttggattgga aaaggtaatg
     4321 aacaatcctt caaccaaaat gtcaggaggc cagcagcaga ggactgcgat tgcaagagct
     4381 ttaatgaatg atcctaaaat gatattagca gatgagccaa caggaaatct tgattctgat
     4441 tctacagaaa caatatatga acttttaagg agtataaacg aagaatacaa gacgacattc
     4501 atagttataa ctcatgacag gaaaattgcg gaaaaagcag acaggataat agaaattaag
     4561 gatggtaaaa taaatctgga tatatacaaa taaggttaag ataatatgaa ttcatacgaa
     4621 cgtgtaattg ctgcatttat gaagaacaaa gttgaccatg tacccattta ctgcggttcg
     4681 gtatcctctt cagttgcatc agaagtaatg ggttatacta tgtatgtcgg aggaggcata
     4741 cagcagtaca gagagagttt agccagattt caaggagaac aggcacacaa agaatttgtg
     4801 gaaaaatcca gacaggatgc atatgactgg actgataaaa tggggctgga ctatgtccgg
     4861 ccttcttact ggagatatcc atacactcct tcaaagatgc tggacgagtt tacttttcaa
     4921 tacgagaggg aagacaatac gcactggata atgagatatt ttgagaagta tgaactgttt
     4981 gactgtatag aggatacaaa aaagcagatt gatgatgtag acgatttgga agaggaagta
     5041 atcattcagg aagaatacaa caaaagatat atgccaaaag aatctatgca taaagagtat
     5101 cttgatgcta tggaatattt taattacaaa aaggcttcat atacaggtgg aattggtttg
     5161 gcgataccga atacaagacc aatctggtat gaagcagtga tcttgcgtcc tgatcttgta
     5221 gaaagatatc ttgatgcctt aatggaaaga gctcttatga atattccgtt aattgcttct
     5281 ataggagcga aaatagtaaa tggcggcgga gactgcgctt gtaatcgagg tcttatatat
     5341 tcaaatgaaa tttttcgaaa tcttattgtg cctagattga aaagaatatc agacaagtgt
     5401 catgaatatg gtatatttca tgcttttggt tcagatggat atttctggga tgtagctgat
     5461 gatttgtata ttgattcagg tattgacgca cattatgaag ctgattgttc ctgtggaatg
     5521 gatatacgtt ctttaaggac taaatatcca aatgtaacag ttataggcgg aatagctgct
     5581 tcaacacttg ataaagcaaa cgagcaagag gttgaaaaag aggttatgga agctattgat
     5641 gctgcgaagg aattatcagg agcaattgta ggctgctcca atcttgtatc tccaacaaca
     5701 ccaataagaa atttcatgct tatgatggat ttattgcata aatataaata agttaatatc
     5761 agtctttttt taaaaaacta attgcatctt ttgcagataa aggcttagag aaaagaaatc
     5821 cctgataata atcacaatca tttcttttaa gatactcttt ctgtaaattt gtttctattc
     5881 cttcagaaac agtcttctga ccaagcttat gggcaagatg gattatatcc gaggtaataa
     5941 gttctttttc atctttaact agtatctggt caatgaagaa tttatcaatt tttataatat
     6001 cagctgctaa ttctctttct cttgacaaag acgagtatcc tgttccaaaa tcatctattg
     6061 cagaacgtat acccataacc tgagctttag ccagctggga gttgatgaat tcaagatttt
     6121 ctgcaaaaac tgattctgtt atttctagta ttagcatgtg agtaggaatt ctatttttgt
     6181 taattatata tctcaaatca gatagaaaat catctcttaa tatctgaata gctgatatat
     6241 ttaccgacac agaaatatct ttttgtccgt tattctccag aagttttatg aacttgcaag
     6301 cttcatcaag tataattcta cctaagtcaa ctattaaatg acgtttttca gcgataggta
     6361 tgaactctga aggacttacg ctacctaatt tgtcgtcatc tatacgggct aatgcctcaa
     6421 agaagcatat atctcccttt ttatctaaaa tcggctgata tacaagatgg aacacttccc
     6481 ttttactatc attacatata ctggctatta catcttttac atcttcttgt cttcgtattg
     6541 attcttccat ctgagtatta taaatactgt atccaaaagg ctctatccct tttacgaaat
     6601 tcgcagccat agtggcattt ttgagcgtag tgtgaatatc aggaattgat ttatcataaa
     6661 tatctaatcc aacccatgca ccatatgatc ttaatgctac attataactt aaaatattaa
     6721 ctatcttttc tgcaacttgg gtcaactctt ttatgttttt gaagttttta acatataaag
     6781 cgaacctatc catggaaagc tgatatagca tatgagtttc atctgttaaa gaagacaggt
     6841 tattaactaa atctaatgta atggattcac agtatgagta tccatatgcg gttatcataa
     6901 gattatatct ttttatgtga ataataatta cagccttact tatgttttct tcattctcaa
     6961 tatcattatt gaaatgctca actaatgatt tttggttata caatccagaa agagcgtcat
     7021 gctcattaac atatcttatt ctgtcttcac tgttttttct gtcagatata tctattatga
     7081 gtccttcaag ttcaataaca ttgtcaaatt catcatatat gccctggccc tgctcataaa
     7141 cccattttgt ctgtccatca gcagtgacta tttcatactc ataaataaat ttctgcttat
     7201 tattaagaac tgttatccat ttatcccaaa tatattgacg atattttgga gcaataatat
     7261 cattgtaaga tacttcagca ttattaataa gactatctga atcgtatccc gttaattctt
     7321 tgcatccttg tgaggtaaaa agcatggtcc agtccttatc aaatttacat ctgtaaatca
     7381 ttcctggtaa attatccaaa agaatggatt tgctgttttc tgattcttta agattctttt
     7441 caataatctt tcttgaagtt atatcttcaa ttatgcaaag gtgatacttg ttatgtggat
     7501 tctcaaagtg caaaggtgat attatcatat gaacccagac ttccttgcca tctggtctta
     7561 tgtacctttt atccatctcg taaccttcaa taatccctgc tttgaattgt ttgaagtatt
     7621 ccaggtcaac acccaggtcg ttaggatttg ttatctctga ccatgttaca tcagcaattt
     7681 gctcttttgg tcttcctagt attttttcaa aagcaggatt tatgcttgga atacctgtct
     7741 gtgtttccac tagatattta aagttgtcag caatagcgat tcctattgta gtctgatcaa
     7801 atactgcttt atataacgcc atactgtctc ttaaccgatt atcagtctca aaaattaatt
     7861 gcttttcttt tttaagttcg aaattcatag aaattatcat ttgaagaaca ataattatgg
     7921 ctaacacaca taaggtcatt atcccaacct gaatagtatt acgaagagta ttcatatacc
     7981 atgtgctgac agggtagtcc aaaccgaaca ctcctagtat ttcattgttt tcgtaatcag
     8041 taatagggac taatacactt acccattttc cccaactatc ttcaaagggt tcagacacag
     8101 taggaatttt ctgaataaag ggtatataat cccattcttc agcttcataa taaatctctc
     8161 ccggtgggga atagtctgat gaattttcag gttctgaatc tacagcaaag tatatatcgt
     8221 catcttttaa catgaatata taagcaaaat gtattagttc attgttttca acaaatctga
     8281 ttaggttttg cttaattatt ttatattcct ctttttccag gtctagctct gatgctgtca
     8341 gtgtaagaag gtatgatttt ggtattaatg atgaagcgct ctttgctaac ataatagttt
     8401 cttgcttctg tgtttctata gcatttgttt gagttttcca agttagaaag gaaccgataa
     8461 tgaatacaag aattaaaagc gcgtaaaata tttttctgtt gtttagtaaa ctagaattca
     8521 tgcctttttt ttctaaacta tccatatgcc atcctcaaat attttaattg aataaaatta
     8581 ttttaatatt ttgatactaa tcataatata ggttatcaca gtttatgcgt tgagacaata
     8641 atataaataa atatattttg aaaatatgat tatatagtgc taaatatacg cataataatg
     8701 tattaaatta ataacttact tactatttgt tcaaatagaa gggtattgct tgattggcaa
     8761 cacttatttt gacaaaaaaa atgagggtaa tatattcctg tatttagtta gatataataa
     8821 acaggttagt ttaatataca taatttacta ttgacactga cgctacgtaa tgctttatac
     8881 tatgaaagag gatgaagagt atgaagctta ctgttaaaaa aactgctgca cttgcagaca
     8941 taagtataag gacactgcac tattatgatg aaataggatt gttaaagcca tcagatatat
     9001 caacatcagg atacaggctg tatgatgatg aagaaataaa caggctgatg cagatattat
     9061 ttttcaaaga attaggattt tctttaaagg atatatcaga tatgatgtca aatcctgatt
     9121 atgacaaaaa tgaagcttta aaaaaacata agcagctgct tatactgaaa aaagaacata
     9181 taagcagact gattatgctt actgaacaag ctatggaggg aaaggtaatg gaaaaggatg
     9241 cagaaaaaat tcagcaatac gaaaaaataa aagaacaata tgcaaatgaa gcaaaacaga
     9301 aatggggaaa gactgatgca tataatcaat ctttaaagaa acacgattca tacaacagtc
     9361 aggacaaaat aaaaatgatg aaagaatcag aagaaatatt caagtctttt gcacagagca
     9421 gaaatcttga tccgaaagat gaaaaagttc aaaacctggt taagagatgg caggattata
     9481 taacaaaata ttcatatgac tgcacaaagc aaattctttc aagtttagct gttatgtata
     9541 cagaagatga aagatttaaa gctaatctag ataaatatgg agagggtaca gcaaagttca
     9601 tgtctgaggc aataatgata tattgtaaag actgataaga ctttttgtat tggaacttag
     9661 cgttttatac atttatattt tttttatata tttgttatta atcactcatc agaaacaggg
     9721 aaatcaggaa ataaaaataa atattgactt actaagaaaa agtgctacta ttatcgtaaa
     9781 atgcggctta cagctgcaat tttttatgga ggtaattagt gtcgacagca atcgatatag
     9841 ggtcagtaac atgcattact atgatacttg cagttttatt taaaccgcag gttaaaataa
     9901 gaaggatatt tattgataca tactggccta ttgcaatgat tggagctttt cttttagtta
     9961 tttttaagtc aatcagtttt aaagaggcag ttttggggct tactgcagat actgcgataa
    10021 atcctataaa aatacttgtc ttattcttat ccatgactat attatccata tttcttgatg
    10081 aggcaggatt ttttcattat cttgcaaatg ctacacttaa aaaagcagaa tccagccagt
    10141 tcatgctttt tacctattta tatcttactg tttctgtact taccatattc acttctaatg
    10201 acataataat tcttaccttc actcccttca tatgctattt cactaaaaat gcaaatatat
    10261 cagctatacc atatcttgta gcgcaatttg ttgcagcaaa cacatggagc cttatgctgg
    10321 ttatcggaaa tcctactaat atatatcttg cgacatcata taatataagt tttattgaat
    10381 atagtaaagt tatgttacta ccaactttag cagcaggaat taccgcattt ttggttttat
    10441 ggcttatatt taaaaaacag ttgaaaaaag aaatcagccc gtcaatagaa aaaattaaga
    10501 tatcggataa gccttcattg attatcggac tgatacatct tggattatgt actttgttac
    10561 tagtgttgtc ttcatatatt aatattgaaa tgtggcctgt ttctctaggt tttgctgtta
    10621 gtcttttcgt atgtattgca gtatccaaac tgattcagaa aaagaaactt gcagttctag
    10681 tttcttgtat aaaaagagca ccatggcagc ttgtcccttt cgttatatcc atgttcgtga
    10741 ttgttctagc tcttgataaa tatgcagtta ctgatgatat atcaggcttt ttaggtacag
    10801 ataatgttat atacaaatac ggtcttagtt cttttataac tgccaatctc ataaataaca
    10861 ttcctatgag cgtcttatac agctcaatag ctgaaaacct tccattatct ttggtaagta
    10921 gaggagtgta tggagctata atcggttcaa acataggagc ttttttaaca ccagtaggag
    10981 cattagctgg aataatgtgg acatctttac ttaaaaagca tgatataaaa tatacttttg
    11041 ctgattttat aagatatggg attgtaatct cagttccaac tatacttgct tcactttttg
    11101 tgttggacat tgtactcaaa taatctatca ttttgggcat attgcatgat atacttaagt
    11161 taatgatatt gatggaggaa ctattttgat ttacacagtt acaataaatc cagcggtaga
    11221 ttacacgata aaaataccag aactggtact gggaaaagtc aatagaagtg aaaaagaaga
    11281 gtacttcttt ggaggcaaag gggttaatgt ttccattgtt cttcacaatc taggaataaa
    11341 gagtattgca ttgggtttta ttgcaggttt taccgggcag ttcgtacgcc agggcatata
    11401 ttcattaggt ataaaaaatg actttattga tcttccatct ggaatgacaa gaattaatgt
    11461 aaaaattaag gcggataaag agactgatat aaatgcggtc gggccggcta taccaaaaga
    11521 ttccattgaa cttttatgca aaaaaataga gaaggtaaaa gacaatgact ttcttgttct
    11581 tgcaggagca gttccaaata atgtagatga gcaaatttat tctgatctat tatctcatct
    11641 gaaagccaaa aatgtacatg taataattga tgcaaccagg aactacctta taaatgcttt
    11701 aaaacataaa ccttttttgg ttaagcctaa cagagctgaa ctgggacaac ttttcaataa
    11761 ggaaattcat actgatgctg atatagtatt gtatgctaag aaattacaag aaatgggcgc
    11821 taggaatgta cttgtctcta taggagacaa aggggctgtt ctagtaacgg aaaatgatga
    11881 tgtcatttgt aatgatgcgc ctaaaggtat aacaaaaaat acagttggag cgggagattc
    11941 cttaactgct ggctttatag caggttatat ggaaaagaaa gattttcgat atgcattgaa
    12001 atatggattg gcatcaggca gtgcaacagc attttctgat acactggcat caaaaaatga
    12061 aattgagcaa atactaaaaa caattgaata ataaaaatac tagaaacaaa tctgttcaaa
    12121 ggtcaggtgt tatccacttg gtcttttttt tctgcccatc catacatata gtttggtttt
    12181 aaaggccaga attttttaaa cagttcatgg aacctgtttg tgcacccgac atcatcaaag
    12241 ttaatacctc tgtatcctgc cttgtatgca ttagaatctt ctgttacaga aaaatcacca
    12301 ttatctatat cattaaatcc tgggtcatca taaatattac cttttggcat atacactgat
    12361 ggttcttctt tactgtactg taacctgaaa gcagggggct taagcttact gtatattaca
    12421 ttgttggata tttccgtaat acctgttgca tcttgaaatt tagatacagt ttttctgact
    12481 atatctgata aatcgtcagg cagagtatat cttctatctt ttgaaggacc ataaaacagt
    12541 atttcatctg ctatgaacac attgttatag tatttcaaat cataacaatc cacaaatctt
    12601 accttcatga gtccattatc aatatgtatg ttgtttttgt ttgtgcatct gtatgctata
    12661 tgattctggc ttgatacagt agcattaaca gaaagattat ttttggctat acagtcttca
    12721 caaagctcgt caaaatagta tgagaaggcc tggacatgat cttcacgatt attcagcact
    12781 acattgtttc ttaatactat attattttca aatataagat aaattgctcc tccgtcctgc
    12841 atctgctgct ttatattcca aatcagattc ttctcaataa ctgaatagtt accgatttca
    12901 tttattgcgc aataaggagc gtcatgtatc tcgcagtgag agattaagtt tttctgaccg
    12961 cctccgtaaa tgccgactgc accaggataa atcttcccta tatcaaaaca ctggcatctt
    13021 tcaatggtta cattttctcc atcatggttt tcaaaaaaat taacagctcc agctcctgta
    13081 tcatgaaagg tacagtcttg taatagtata ttactactgc attttttaat tcctattccc
    13141 catgctgaaa catatgacaa attacaattt tttattgtta tgttttcaca agatgttatg
    13201 tctacagcag cagataaatg tctggcgcca tatccaggat taataaggtc gttattggac
    13261 atttggatat ccaaaccgtc gattatgaca tttttagcat tacttagtga taatatatta
    13321 tcaaacacgg gaaacacccc gttaatagtt tcatctatat ttcctttata ccaaacgagt
    13381 cctttattaa aatcggcata ccactgatta atatcatgaa gtccttcttt tgtattaaat
    13441 accacatatt tatcagccat ttcgtttctg tcagcaaaag ctccagcagg catgtctgtg
    13501 gaattagtaa gataaaacat gttttttgtt tcatcttttg ctataacacc tgctaaagta
    13561 gcactccact catgatatat gagaagttct gcagaatcag tatctaaacc tttttcgata
    13621 tctttttctg agtatttaat actacgggac tgagttattg taggtttagg gtcccagtgt
    13681 ccgtaacagg caggaagaca gtcctgattc cattcacaaa ggtgtttaaa tacgccagtt
    13741 tgcggtaatc ttgatctttt agctaatttt tcatttatct ttaaaagcct gaatggtttt
    13801 ttctgaaaat gtccattatc ttttgaagcc tgccaaaaat cagataccag atagccatct
    13861 acagattccc atttgctcaa aggaaatcct ccatagagta taacggtacc tttattagcg
    13921 gatattttga gattattgtc ttttttgcac aaacttattg aaacaccaaa atattcacca
    13981 ggcagaatga aaatatcttt tatacctgtt tctcttgaaa gaagaacggc tttattcaat
    14041 gttttcaatg gactcttttt tgtcccgtta aattcatcat ttccattaac tgatacatat
    14101 attgttttca tgaagtcctc cgattataaa aatctataca tcatatatca aactatactt
    14161 tataactatt gaaataacaa catcacaaac catgtaagat taactttaca gtaaatcatg
    14221 caactgatat attaatcata ttcttatggg ggtaatatat gacaaaggtc tgtataaaca
    14281 aaggctggat gttgcagcaa agaccattaa atgaaaaaga atggtctgat atggataaat
    14341 ggggcaaaca gtataaatgc gatgttcctt gtgatgtagc tatgccgttg atagaaagcg
    14401 gtgatatgcc cgatccggcc atagcagata attgctataa aaataaatgg gtagaagaca
    14461 ggtcctggtg gtttcaaaaa agcattaata cagatatgct gcctttatgg caagattcag
    14521 atagtgtaga gcttgtgttt ctgtctttgg accacgaggc tgatattttc ttaaatggca
    14581 tccatatcgc taatcataac agctgtcatt atgaattcag gaagaatata aaacggtatc
    14641 ttaaagaagg agacaactta ataacagtaa ggcttacttc agggttggaa acgataccaa
    14701 aagaagagct ttttgaagta gacaatgcta tctgtaccga agaaccgaga cgaaatgaca
    14761 ggggagataa aagaagggca tttttaagaa aaccccagta tgtttatgga tgggactggt
    14821 caccaagagc agttactgta ggaattgcag atgatgtata tcttgaaata tccaaaactg
    14881 caagaattac tgatgtatat atatacactg aaagcataga aaacaattgt gctgtaataa
    14941 aaggagaaat tgaggttgag aatatgaata tactttcaac tttggatgct gaaagtataa
    15001 gaatagatat atcgctaaac gatgaaataa aggctttttg gaaagaaaaa gatgtctttt
    15061 tatgttcagg actaaactac attccttttg agttgaatat agaacaaccg caactttggt
    15121 ggcccaataa ttcaggaaaa cagccattgt ataaagttaa ttgtaatatg aatataggaa
    15181 aaagacatat atcatttcct ttgtttgatt ttggaatacg taccatacaa ctggatactg
    15241 ccaggataag caaagataga agggatttca agtttaagat aaatggatat gacatttttg
    15301 caaaaggagc aaactggatt ccttcagacc ttctttacat gaaagtggat gatgacaaac
    15361 tggagaagat aataaagcaa gctgcggacg ctaatatgaa tattcttcgt atttggggag
    15421 gagcagtata caacaggaag ttattctata cattatgtga taaatacggg attttagtat
    15481 ggcaggattt catgttcgca tgctctgcgt accctgattc gcttgaatgg tttatggatt
    15541 tatgtgaaaa agaaattgat tatcagacta aaaggcttag aaatcatgca tgtttagccc
    15601 tgttttgcgg aaataatgag tgtcatgaga tcttcagcat taagaacttc atgaactggg
    15661 acataaatcc ggactattca aaacagtatg gtcttagtat ttcaaataac cttgcaaaga
    15721 aaataacgca caggaactgt ccgaatattc cttgctggaa cagttctcca tacggaggag
    15781 acattcctca aagctcgaaa tgcggtgatg tgcattactg gaacgacgcc atgatgagtc
    15841 ctgatatgga taacagaatc acattacaga tatatgataa ggtgtcttca gctttcgtat
    15901 cagaatatgg gtatataggg ccaacatgca aaaaaaccat agagcagtat catggtgact
    15961 gtcctattaa aaaaggcagt ttcgtatgga atcttcataa caatacaatg gagaaagata
    16021 ctgtagatgc aggtataaaa aaacattata ccgacaaaaa actaacgctt gatgagtata
    16081 taacatatgc tcagatggtt cagtactcgg tacttaatta ttctttggaa gcttttcggt
    16141 tcagtctttt gtgtaatggt ggaattttct ggatgttcaa tgactgctgg ggagaagtag
    16201 gctggacggt tctagactac tatttaagaa gaaaagccag ctattatggt gtaaaaagag
    16261 catttgagcc tgtgaagata ataatccggc ttgttgataa tactataaca gcagtaggta
    16321 taaatgagac tgataaggat atgggtttta ctgctaaaac cggatggatt acttttgatg
    16381 gtataaaaaa tgaaacggat aataaaactt tcagaatacc aaaaaggagc cgccaggtta
    16441 tacacatgga aaaaatgcag aagaaagatc cgtctgaatg gatattttct ataataccaa
    16501 tagcaacaga aatttcttca gctatactta atatcgctga tgcttgcata agaaagacca
    16561 aaaatgatgt tgatattctt aagtatgaga atgacaatga tgaccttcat gtatatctga
    16621 aaactaaggg atttgttcat gggatatatt ttgaagagga ctataattac tctgacaatt
    16681 actttgatat gctgcctgat gagacaaaaa aggttattgt ctatggtgca aaaaacaaaa
    16741 gactgatacc aaaaacagta attctggaac atttttagag gactacccgt taaacttgta
    16801 ggtactttta atgggatata ctgctgatta gtggtataat ctttaaacag gtatcagaag
    16861 attgcctgaa atgaattgat atgaataata aaacaaaaac aacaaaagat accaagaata
    16921 caaaaagaag aaaaaacaag aagaatacag cgatactgat agtagttgcg gtacttcttt
    16981 gtatcgctat atcgctttta tatgccttta tgaaaggctg gtttgaacct aaggttaccc
    17041 caactccatc taatagccag tcagcagagc cttccataag cgtatcgcca cctccttccg
    17101 agactgaaac tgaaactgaa actgaaactc cgactgattc acccataata gtaaatccta
    17161 taccggaagg tcatatcaag attgataagt ttgatgatct taaaggatat tttgaatata
    17221 tgctatcaac aaagacagga atatatggat tatcggttct tgatgttaca aataataaat
    17281 cattcaatat agctacaagc cagcagtttg ataattcatc agtatacaaa atagctgttg
    17341 ttttgtatgc atatgagcaa gaagtacgac aaggaatgcc tttagataca ttggttgagt
    17401 ataccgctga cgactatgat ataaattctg aagtggtagc aataggcaat tattcagata
    17461 ctcatactat acaaaagtta tgtgaatata tattaaagag tgatgacagg gctgctctga
    17521 atatgctatt aagagtgtat gggaaaagta atattgaagc atatatgtct agcatgggtg
    17581 tatcagaatc aatgtcatcg ttttcaccta atgatatggc aatctttttt gataagctaa
    17641 aacagaaaac tgatgcatca attgacggtt attcgcaaat ccatgaacaa atgaacagcg
    17701 ggatattaga tggagtgtca acagcttctt tatttacgaa agatgaagat attttaagca
    17761 tgaatggttc tgtcattgta tatgcaaaaa atatatttat cgtttcaatg tcagctaagg
    17821 atgcgaatat ggctgaagcg gaaaatacct ttatggacat atattcaact ataaatacct
    17881 tatttactga aaatgaggtt atagtagaaa aaagcatata cagtcatgcg cttgtcctgg
    17941 ataatattgt tgaatcagca aaacagacag catctaggtt taagtcatac ataatgatgc
    18001 ctgacaagac tgttacatct tcatatgttt ctgattatga tatatcattt cctcgctcgg
    18061 atgaatattc cgatattggt gttactacat acaggggtaa taactacaga gattccggat
    18121 tctttggtaa tataaacatt tcagaacaga aacttgaaga aatatggagt tatgagatag
    18181 gcggtatgac cggtgccggt tccatatggc ctggagtggg ttggactggc cagccatgca
    18241 tagttaaatg ggataaagaa atgctttgca tgatgaatgt ttatgataag tttaaaaatg
    18301 agacagagct tattgaagtc atttatgcca cacttgacgg taacatatat tttcttgatt
    18361 taaaaacagg agaacctacc agagactata tatacatagg agttccaatt aaaggaacat
    18421 tatctattga ccccagaggt tacccccttt tatatgtcgg ccagggtctt gaggctgcaa
    18481 aaacaaatga tggagaagaa agatacaaag cttttgggta caggatattc agccttatag
    18541 attctacact tcttcatgaa ataagaggat atgatacatt cgctccaaga ccaggctggg
    18601 gagcatttga ttcctcaggt atactggata ttaagaatga tacatattat gaatgtggag
    18661 agaacggagt catatatata ataaaactta atacgatatt taataagaca gaaaagactg
    18721 tatcagttaa tcctgaaatt acaagatata aatacatata tgagtctgag tacgcatatg
    18781 gaatagagag ctcaccgtca atatatgaaa aatatatgta ttttaccgat aatacaggaa
    18841 tgctcatatg tcttgacctt gaaagtctta gaatactatg ggcatataat acaggtgatg
    18901 atactgatgc atcggttgct ctggatgtta cagaagaagg cgtatttcta tataccgcaa
    18961 atgaagtgga taaaagagga aaggacgatt acagtaattt acgtaaattc aatgccctta
    19021 caggagagct cttgtggcag gtagatgtac ctgtatatta tgttgatgtt ataaacgggg
    19081 gagcgctggc ttcacctatt ataggaaaag gaccaataaa ggatttagta atctttaata
    19141 tatgtaaaac gctggaagat tccatatggt caggaagact gatagctata gataaagaaa
    19201 ccggacagat cagatgggaa tataaatccc catatagctg gagctcacca gtagatatat
    19261 acacagaaga taacaaagca tatatcgtat actgtgagca taatggtaag atatccttga
    19321 ttgacgcttt aacaggaact cagattaacc aggttacttt gagtttaggc agacagtata
    19381 taactataga agctactcct gctatatatg gagatatact ggtagtaggt acatatgccc
    19441 aaaagatata tggtgtgagg ataaagtaac atgacaataa aaaagagaaa aaacagtaca
    19501 ttagtagcaa tagtcattag cgcggcagct ctactgcttt caatagctaa tttaatcctg
    19561 tgctacattt acgatttgga aaaacctctt gcaacgataa ttgtagtatg tatatctgca
    19621 gtgttctcac ttaatgtagc atactatgtt tcactaaaga aaaaaatgaa aagggaaatg
    19681 gaaagaaaag atgaaaaaga caagtaaatc agatgatgca gaacttatga agcagcagtt
    19741 agcagaactt gtaaaagatg agccgtttgt catacccaat ctggcaaacg catcagcttt
    19801 cataatggat acatatatag acctgaactg ggcaggattt tacctgctgc atgatgatac
    19861 attgatcctt gggccttttc agggaaagcc tgcatgtata aggatagagc ttggtaaggg
    19921 agtatgcgga caatctgcaa agctggataa gacactgaat atacctaatg tacatgaatt
    19981 tactggccat atcgcttgtg atagcgcatc taattccgag attgtaatac ccatacactg
    20041 taataataaa gtgataggcg ttttggatat ggacagcccg atattttcca gattcacaga
    20101 agaagaccaa aaacagcttg aggacctggt aaagatactg gaagagcagg ttttttaatc
    20161 gaaattcgat taatttatct ctcgcactga ttcttccatc tgtaaagcca gtcaagctgt
    20221 ttgtcaagga aagcgttatt tacacagtca agaccattgt tttcaatgac tgtcgcttgc
    20281 aaagcgaaat gatatagagc aataagatca taaaatgcat ttatttctgt ttttgtgagg
    20341 gtactgaatt ttaagtatcc tggaagaacg tgagcaagta tttcttttga ctttaagtat
    20401 cctgactcat caaaattaaa atagtgtgtc ttgttgcata tcaaagcaat gtcatacatc
    20461 ggtaatccgt aacaggaagt ataaaaatcc aatatataga attttctgtc tgatgtcttg
    20521 tatatattcc cgtcatacat gtctccatga cagtatccgc gcggtaaatc ttttacactg
    20581 tcccaaagtg tattaccata ggttacaaac tcatctgttt tcggatattg ttttctttta
    20641 aggatgtcta tgtatctgac aatatagaac tgtctgtcac aatccttaag ttcaccagta
    20701 taattcttca ttgtgttatg tagtctgcca ataagttctc caatagcctt tgcatcctgt
    20761 tcaggattcg aatcctttcc ttcgataaat tcatatagga tgaataatcc gttgtcagtt
    20821 cttatgcaag gttggttatc ttttgtgtaa ataataggag gtactgggaa tcctttacta
    20881 cataggaagg cttgtatatt agcacctgaa aatgcggtat caataaatga tggcttaatg
    20941 actcgtagaa aatatcttaa gctgcctgag tatacagtat atgaaacgct tcctgcatct
    21001 cgtatcttac tgatatcagt gaagttcatg tcgtaaaagt tatttaagat actcaatata
    21061 ttattcattt tatatttact ctttatttcg taatgtaccc atttaactat atttgcatat
    21121 tcgtgtcaac tggtatagat ttgcttagag cataggcatt actagataaa tatttttgga
    21181 ttataaaaat attttcaaaa acttgtcacc tatatgaccc tttttttcgt tatatatagt
    21241 atagaggtaa aagtattcca aaaatgcaga atttttactg aaattgtaaa ctttttatga
    21301 acactcttga cattttattc tagcagtagt attatgaagt tacagaagtt atattaatat
    21361 ttatcaagca tttgtattat tttatgaaat tgaaggattt atatgaaaaa gagatttata
    21421 gtgtgtttta cattagtatt tatactaatg tctgttattg gtcttacatt gcttacaggt
    21481 ttggctgaga gtggtcatga tgaaggagat tctataaaag aagcatcaaa tattgctatt
    21541 ttaagtatag acggagtaag taaagaatta cagtttaata gtgtgactcc gtcagggcca
    21601 aatagtttct atgtggttaa tgacaatgaa gtatatattg ctgatcaata tgataaaaaa
    21661 ataaattact ataaaaataa aatgcttgaa aaagagatac tctttcctga tgttaatttt
    21721 ataactgatt tctatattgc tgataatgaa ttatttttac ttgatgatta tttttctgga
    21781 gcatatcgca taaatgatgt catatgcgca gacatggatg gcaatatcaa atacaaaatt
    21841 aatataccaa atgacagtac tttcctcgaa atgaaaaatg caaataatta tgatagacga
    21901 tgtagggtaa gaaggattta tgatacaaat agttacatta catgcgagtt ctctaactca
    21961 ataaattata tttatacaga tagtgagtgg atagaatata aagatgatga tttgcagaaa
    22021 aatatcaata ctcatatggt tacatttaac aaatggagca attcaataat acaatcgcaa
    22081 ggtgagatag aatatgcgga cgttattaag acaaataagg atgttttgat agttagcgta
    22141 ggagcagtaa ttaaagaaga aagcagttac tctaaaatgc aatgtctgta tatgattaac
    22201 gaaaagggag ttagctgggt tcagcctgta aaaactaaag ctaattcagt tccagataag
    22261 caattcttca tatctgagga taatcaaatt tatcaaatgt attatgacga gaataataat
    22321 acatttatta gtaaattaaa tcctggagag tatagtatca ggtattcagc atcagagtca
    22381 gatccgaaac atgaatcaaa tgatatttct aataataaaa tcactttggc atcatattca
    22441 tctattacaa gagagcaaat agaaaataga gcaacagatt ttctaaacca tacatggact
    22501 tttaattgct atacaaatgc agatgatagc attcttgtag gttgtacagg tgtgtcagtt
    22561 gcatgtatta cacatcaaaa ttataatatt ttacctgttt ttttaccaca gattaaatgc
    22621 acaaattcat gcaatggcca cactgtaacc ggaatccctt actgctggac tggacaatgt
    22681 gagatgcgaa tacctgcaga tgcaccatat acacaccttc agttatatat taatgatgga
    22741 actacagctt ttgatgagca aattagtaat ggttattatg cgggaaatat tccaataaag
    22801 ggtaatcttt ttgatgttag aaatcattat tggcttttca aaacagcagg agtggattgt
    22861 tcaggattca tgcaggtatg ttttaagtat aatgattctg caaaaataaa ttgcaattgg
    22921 ataaagaata caacagatga ctttgattct atatcaagtt tagcaaatgc caaagcaatg
    22981 gatttattgg ttaattctgg tcatgtaatg ataattgatg aggttgaatg gaatagtaca
    23041 tatgatcaga taataagagt atgggtatat gaatgtacga ctgcaggtta tgataaggtg
    23101 atatataatg attatacgcc atctgaaatt tcatataaaa attacgagat tagaagatat
    23161 aaaggagtta ctacttgtac acactcatgg agcgattggg cagttgtcgt atcaccaaca
    23221 tgtatatcaa caggaaaagc agttagcaca tgttctatat gcggtgaagt tgatacatat
    23281 cctataccaa taattgcaca taactataaa ccagcgacat gtgaaagtcc gcaaacatgt
    23341 tgggtttgca atgctacaaa cggaacagct ctcgggcata acttcacgat atatcagaat
    23401 acatcatatc agccatacag cagtactcag catatggtaa tcaatcagta taagtgttcc
    23461 agatgcccgg aacttgatga cgagatacaa tatgcatcac atacatataa tattccagca
    23521 gccacatgtg atacagcaaa atactgtaca gtatgcggaa atgtggctca gcctgccatt
    23581 ggacatagtt atagtacagc aacatgcgta tcagctgcaa cctgctcaaa ttgtggagtt
    23641 actagcggtt cgaagaatcc aaacaatcat tcaggtggaa gccatactga agtaagcaac
    23701 ccgacatgta ctgttaatgg ctctcagagt ctggtatgtg acggatgcgg agcaatacta
    23761 agtacgtcaa caatccttgc cccaggacat agcatgtcag catggtcggt tataacggcg
    23821 tcgacatgca gcacgcccgg cactcagtca aggtactgta ccaattcagg atgtggttat
    23881 agtgaaagca gttcaatacc ggcattgggg catagctgga gcggatgggt ataccagtat
    23941 acatattatg atcctgactg gcagagcaat gtacatgtat ataggaggac atgcacaagg
    24001 tgcggtgccg ttgagtatga ctatatataa ttaagttgat aaagaatagg attaaataat
    24061 atgagaaaag caataagcgt tttattaata ctggtatcac ttttagcatt atcatcctgt
    24121 gatgaagtcc aatcaggtac aaatacccct gctccttcac tgactgagaa tacaactgat
    24181 acagctgttg tgccgagttc tacggatatg gcatctgaaa ctgcattgcc gactgaaaca
    24241 gttgtcgcac caacccctac agcaactcct tttattacac ctactccgac tccaaatcct
    24301 ggtcccagga atatagagct ggattttttg aatgatagat tcgataaggt tgaggcttta
    24361 attgatggaa gctacgtgga tgtaagcgat atatgggaat cattcaagca gataatggga
    24421 aagccggtat tcagatactt tgatgcgtcg gatcttatga ccattcaaaa caagattcgt
    24481 ttcataaagg atggcaggga gtttatgctt agtccattaa agagaacgag tacttcatat
    24541 ggaggttcag attatggaac ggttagttat caatggtcaa atactatact gcacagcagt
    24601 tattacattc cgaatatttc acttttttat acgattttga atatggacta tcttgaaagg
    24661 gaaggcgaaa caggattggt cctgaaggaa tatttcagtg gagatgtgtt ccagaaaatg
    24721 tatgacagca cagtgttgga atatgacggc tcatatatat ggacgccaaa gaaggctgtc
    24781 cccatataca atatagctaa acatatgaag aacagaatgg atttaaaacc tgatgagtta
    24841 ggcttatata ccatagtgag gagttataca tacatttatt ctttggaaac acgcatatcg
    24901 ctggacatct acgggaaaga tgaatcaggc aatggtgtat atgccaatgt aaaatgtggt
    24961 gatgacgata tatggatggt tcttgactgc tccatactgg atttcgataa cgggcttagt
    25021 gtaaatgaca catatcatgt accttcaggg gcgatccccg taagagatgg cgagtggttc
    25081 gatggatatg agaagataga gctggtatat ggggaggcta ttactgatgt gactgagttc
    25141 tggtacagaa accctgattg tgtaaagata tgcgactggg agacgtactt aagcgggcct
    25201 gcgctacctg tcgaatatga gtacaggatg acaaagaacg gcactgtaaa gacgatgagc
    25261 gtttccgata tgtacgacta cttcatatca gaaggtgatg gtctgtacta ttgcgattat
    25321 cttacatcat acaaaaacgc atttatcatg accgaagagg aagcagtaat agaggatttc
    25381 atcaacagaa gcatatatca gaagatgttt ctatgtgcaa tcttggatat aaccaaagaa
    25441 aatgaggatt atagaaatat cctaatgaac cagaaagctt gtcagtttgc aacgtcttta
    25501 gcatttagaa tcaaagagca tcttaatggt aaacctgaca tggaaatagt aaggaggtcc
    25561 gaatcaacct gttattataa aggtgaggta atatcagtca tagcatggga cataccggga
    25621 gatgggtata atctagggta tgtgaacata ttaagcggtg atacggatat atggctcaag
    25681 atacagaaca tacaattcct actcggcttc cttggtgtta atgactggtg ataaggggta
    25741 atggtattgg tagttatata taagaattaa aataattaat tatttctaaa atatgtcact
    25801 tatatacctc ttttctatgt tatacatagt gtaagagtca aaatacatac tcaaaatacg
    25861 tatctactga agttatctac atatttgttg ctatttctta aataactatt gtatttgtga
    25921 tattatatat acgaaacaag gatagtttta aggggcgcac atgttcaata atatgaagat
    25981 cgctgactgc catatacact attcgtctaa tatatctgta gatgaaatgg caaaaatatt
    26041 tgactattgt aacactgaca tagccaatat cgttgttgtg cctgataaac tgaaattatc
    26101 tgctgtacaa gatgccctta tacttaaaca taaattacaa gacaggatat atgtgtttgc
    26161 aagcctggat gcttcctgtt attttatcaa taagaaaaca ttaggaaaat caatggcaaa
    26221 gcatgcgttg ctgatgagaa aacttggatg tgacgggata aagatgatag aagggaagcc
    26281 tcagataaga aagatgctaa atgtaccttc ctttgattca ccagtatggg aaccatattt
    26341 taaattcatg gaagacacgg cttttcctat tttatggcat gttaacgacc ctgaggaatt
    26401 ctggaataag gataaaatac ccaaatgggc tttggagcag gactggcttt atgatgagac
    26461 atttattaat aatgaaaagc aatatgagga agtgttaaag gtattaagaa ggcatccgaa
    26521 cttaaagata atattcgcac atttcttctt tatgtcaaag cagttagaca gattatcttc
    26581 tatactggat aagtatccga atatatatgt tgaccttaca cctggaattg aaatgtatat
    26641 taatttttca gagaacaaag aaaaagctgt agctttcttt gaaaagtatc aagataggat
    26701 catgtacggt actgatattg gaacaaaagg ggtagtggat ggtaagagcg cttctttgga
    26761 agaagaaaag aaaagagcag atcttgtcaa aaggtatata tctgctacag aggattttct
    26821 ggtagataag caatattcat atcttatgca tgataaggat ttcatattga gccctctggc
    26881 tttaagtaaa aaaatacaat cgaaaatatt cgcacagaat tttataaatt tcataggtca
    26941 tgttccatat gcaatcgatt caaaaaaggc gttatgggaa gttagaagaa ccatgttcaa
    27001 acttctttta atgaaaatag gggataaaac tacaaaatac gatttcaaat cactaaagaa
    27061 agcttgtgaa caactaaaga gaatataaca aggaggtcaa gatgcagaag atcaagagaa
    27121 agcacctgcc tttatacgct ctatcaggct tcgggcccgg gctagtaaat ctgatgatta
    27181 cagcctatct tatcgacgcc atgagtacag caggtttttt gaagaacatt gaaaattgga
    27241 catatgcgaa taaaactatt gttgcgacag ctctattcag cgtgttcgta ttgattgcaa
    27301 aggttgttga cgggattgcg gatgttccgc ttgcatcgct aacagatact ttgaagacca
    27361 gatggggtaa aagacgccct gcaatactga ttgggtatat acccatgatt atatcgttca
    27421 tacttttctg tttccctttg gagacagtgg aaaacagcat gatgaatacg gtgtggttcg
    27481 gtatacttct gattcttttc tttacctcat atacactgac attagtcaca tattatggga
    27541 cttattcaga nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    27601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ttatccggac
    27661 agaatgtatt agcatcagga ccaatggggc ttaactactg gcagataacg attataaaca
    27721 ctgctgcatt tgcgcctgta cccttaatgc tatacttcta taggaagata atgaaaaaga
    27781 agggtttcag gtttgcattc cagactgcaa tgggttcatt cgcagttgca atgttaatgt
    27841 tctcagtagc atatatcgaa tggataccta acgagtatat acgtcttgct atagcagcaa
    27901 caggagcaac tattggaagt tacggtcttg gagcattctt ctcagctcct tatgctatac
    27961 cttctcaggc agcggctgat gaattaaagg caacaggcaa aagccatcca tctatgtatt
    28021 ttgcaatgca gggattatgc acagcccttg tcggagcact atctaccagt atcgtttggc
    28081 ttaatattaa agagataacg cttccagaca atccggtatt cggagctcat ctcatgccat
    28141 atatagttat gattgcatgt gttgtagcca taattgcagc gaatttcatg ccaaaagagt
    28201 ataacgaaat ggggaaggaa aaatgagtgt tgataatagt aattctgtaa agttatacct
    28261 ttccatagac ataggcggaa gtgctatcaa gtatggtgtt cttgatgaaa cattttcttt
    28321 tgtatataaa agcaaaatgc cttcaaagat attcttagga ggaaaagagc ttctaagccg
    28381 ggtaatgagc atagtagcgg atatttcaaa aaaatactca atagaaggtg tcgctatttc
    28441 tactacaggt tctgttgaca gtaaaaatgc gaaagttttg tatgcggatg atgtacttga
    28501 agactatact ggtacagatt acaagaaact tattaagcag gaatttgatc ttaatgctgc
    28561 tgcctgcaat gatgtgaact gtcttgcttt atcacaaatg aagtttaata aagggaaaga
    28621 tttcatagtt gtagctatag ggaccggtat cggaggagca atagtaattt ctgataagct
    28681 gtatgaagga tattgctatt cagcaggtga attcgggctt atgcatttaa gggacggtaa
    28741 aaactgggaa acattatgtt ctatgagcgc attggttaaa tcctcaaaag agcataagct
    28801 tttaatatca aacggcaaag agctttttga tatgtttgat gctaataacg atgttgcgta
    28861 taagatagtg gattccttct acaaggatat ggcttcggct cttgcaaacc tcatatatgc
    28921 attctctccg cagaagatta ttattggcgg gggtataagc gcaagaggag acagtttaat
    28981 aaatggtgtt aaaaaatacc ttggtataat gatggatgaa ttctattatg aaaagaccag
    29041 tatagaatgt gcagtatatg aaaatgatgc tgctatgatt ggcgccttac agcattatct
    29101 tttcatgaac cataagtagt gagatactgg ctgaggccag tcatggtcta taaattcaga
    29161 tagttcagga cattcagatt tgtctaatat attgaatgca catatcaaag gccagaaata
    29221 gaaggacata tttatcatat tgccccagtt agctggagac aaagtaggta cagaattgaa
    29281 gttgtgcccg aatcctacct ccattctgga ttccaatacc ccgtaatcat taatcttata
    29341 cttaaatatt gataataccg ctttcttaag cgctttttta atatcctctc ttctgtaatc
    29401 aatgcggtta tagttaaatg ccaataatgt catacaatca gtatctattg tgttgtcatt
    29461 gtatgataat gggtcatctg gtgctcttag gccgaacaac ccgtcatttc tttgtaaaag
    29521 aagtatcata tcagtttgtc tttttctaag ttcatctgga atgtcgtggt tctcatatgc
    29581 ataaagccat aactgatggg cgctgcccat tatgggagag gatatccagc aatattcatc
    29641 agatgtcttt ggtgtcagag tctcaaaacc tgtttccatt acatgatgcc atatcttttc
    29701 tttatcagtt ttaaggtctc cgtaccaaaa accagttata cctatgttct catcatgcca
    29761 tttgtaccat atctcatcaa gaagtttacg agctttatcg ccctctttag ttccttcttc
    29821 ccatgtagca atctgccata atatgcaacc tgcattccaa aattcatttc ccatccacca
    29881 cgggttgtgt cttcgaaaat taagatcgaa cttatagttg ttccagtcac ggttatacag
    29941 gtaatctttt aggaaatcct catcaagata tcttcctgtc atagaacctt tagagagttt
    30001 gtacttggat tttgctccta aaagcattat agcagggcat aagcatgcta ttaagtgttc
    30061 atgcatgtga taccaaggat gagtatttac agaagcatct gaccaggaag ggtctgactt
    30121 gtcaggtata tatggaccta agtaataacc ggttgcttca tcctgaaagc tgtttatata
    30181 atcagcccag tttgctttat cctcatctgt aagtgtatcc agctctccta aaagatacag
    30241 agcttcaata gcgctgtggt ttccctctat gctttcaact cctcctggtg tggaggaaaa
    30301 agcccagtag ttgttttttg cacgctcctt tagagtgagt gttttaacaa tccttttttc
    30361 tttgggtatt atgaatttat cggatcctga gcctcttacg ttttttccgc ctgctgcttt
    30421 gaaggcttct tcatcaaata caccatcaga tggtacacag cgttcaataa atcttaaaac
    30481 gttatttttg taagattcca acaatagaat tttatctgtt ttctccgtat tgtccatttc
    30541 aaaccctata gtatcttgca cacatcctca aagtgccttt tcggaacaca aagagttcct
    30601 tcttcatcaa agtaatatct tacgctttta tcatatttca ttggctccgc tcttgatgtc
    30661 atagcgggat aaccgaaaga tatgagagag tgaagagaca gttttttctg gtctactcca
    30721 catatattac agatattatt cttgtcgata gccatcatcc agcaagagct tacgccttca
    30781 ttctgggctg ttaatatcat gctcattgct gctgcacctg ctgcaacatc atctgacctt
    30841 cttatactta aatcattaag tataagggct attgctgttg ggccttcctc taatgatggg
    30901 gagcattcgc cgtcaggcat atatcctgca tatttggtat atgggaatat cttatcaagc
    30961 agctgtttat ctgtaactac ataaaatctc aaaggctgca tgttcatgcc ggtaggtgaa
    31021 agccttgctg cattaagcat tttatctatt aaaggccttg ggataggttt ttgtgtgaat
    31081 ttgcggatgg acctgcgttt taaaattgta tcatatgtgt ccattttttc ctcctgttta
    31141 gataattgta ccatagttgg attgaacata ttgcgtttgt ttgttaatat attagtatag
    31201 gtgaattata tgatttataa aggcgaaaaa acgaaagaaa tatcctttcc gttaggcgga
    31261 ataggaagcg gaagtatagg gattgccggc aatggtagtt ttatggactg ggaaattttc
    31321 aataagccgg acaaaggctc aaccaatgga tatacccata tagcggtaaa ggcagaggat
    31381 ataaacaact ctcttgtgtc agctaaaatc cttaactgcg atatggataa agagtatctt
    31441 ggccgttaca gcacaagcgg aggtttcggt tttggaatgc tgaaacagac tatggcaggt
    31501 ttccctcatt ttaaagatgg tgttttcaaa ggggaatttc ccattatgga gttatcctta
    31561 aaggacaaga cctttcctgc aaaagctaag ttaactgctt ttaatccttt tataccgcta
    31621 aatgaagatg actcatccat tccaggggct ttctttgaag tagaacttaa gaatgatact
    31681 gacaacccaa tatattacac tgtctgcttt tctgtaaaga atccatatat taaaggtata
    31741 aataaatata tgcctaattc taaagcggaa atggtattta tgaagaatgc agataaacca
    31801 aaagaggata tcgagtatgg agatttatgc gtgtcattag ttaaacaaaa agacgcagag
    31861 acctcatacc agactaactg gttcagaggc ggctggcagg acagtataga gacattctgg
    31921 aatgatttca caaaaaatac gattttacca aaaagagaac atagcgatgc tggcaagtat
    31981 gatacctgtt cggtatgtat aagaaagcta gtggaatcaa aaaagagtat gaaaacacgt
    32041 cttcttctta catggaatgt tcctaataac tacaattact ggaatccata tatgaaggat
    32101 gataaacatg tgacatggaa gaattactat gcgactcttt ttaaggattc atgtgcatca
    32161 gctaagtatt caataaaaaa ctggaacagg ctgtacaaat caactaacct gtttagaaaa
    32221 gccatttttt catctactta ccctgactat gttaaagatg ccatatcttc gacactgtct
    32281 gttttgaaat ctcctactgt tttaagactg gaaaacggtg agttttacgg atgggaagga
    32341 gtagacgaaa aacagggttc ctgctcaggt acctgcactc atgtgtggaa ttatgcttat
    32401 gctttaccat ttctgtttcc aaaacttgaa aggtcaataa gggatactga ctataaatac
    32461 aacatggatg aaaatgggaa gatgcagttt agaatgaatc ttcccttagg ccgtgacagg
    32521 catcaatgga gagcatgtgt ggatggtcag atgggcggag ttataaagac atacagagac
    32581 tggaagatat ccggggacac gaaatggctt gaaagcaact gggaagctgt aaagaaatct
    32641 cttgcttatg cctggtcaga aaaaaacgaa gacggttggg actgtgataa agacggggtt
    32701 ttggaaggac gccagcatca tactctggat atggaacttt tcggaccgtc agcatggtta
    32761 gaaggatact atcttggagc attgaaggcc gcatcggaaa tggcattcgc tcttggagag
    32821 cgggaacaag cctgggaata cgagaaatta tacatgagcg gtagaggctg gagttttgac
    32881 aacctgtata atggcgagta tttcatacag aagattaacc ttaaagacaa aaacttatta
    32941 gaaaaatatg attctgtgga cagatactgg aatgaggaca aacagcagat taaatatcag
    33001 atagggcagg gttgtgatat agaccagtta tgcgctcagt ggcatgccaa tatatgcggt
    33061 cttggtgaaa tattgaataa taaaaggaca aagaaagctc taaaggctat gtataagtat
    33121 aactttaaaa aatccatgag ggatgttgta aatccctgga gaatttatgc agtcaacgat
    33181 gaaagtggtg cagtaatatg cgattatcct gcccatgtgg aaaagccagt tctgcctgtg
    33241 ccgtataaca ctgaatgcat gcatggtttt gagtatatgt ttgcaggtct tctcatatca
    33301 gaaggattaa ttgaagaggg tttgagtata gtcaaatcag taagagacag atatgacgga
    33361 agaaaacgaa atccatggaa cgaggttgaa tgcggaagca attatgcccg ttcaatggct
    33421 gcatatgcat tagtcccaat attatccgga atgacatttg acatgacgac atatacatta
    33481 gggtttgacc ctaaaacttc tagagaag
//
LOCUS       DAOSXM010000015        33130 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2111
            NODE_858_length_33130_cov_10.217989, whole genome shotgun sequence.
ACCESSION   DAOSXM010000015 DAOSXM010000000
VERSION     DAOSXM010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864178
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 33130)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 33130)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:24:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,261
            CDSs (total)                      :: 2,229
            Genes (coding)                    :: 2,218
            CDSs (with protein)               :: 2,218
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 3 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33130
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_858_length_33130_cov_10.217989"
                     /isolate="AMR_MDS_2111"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(107..1174)
                     /locus_tag="PLQ68_03635"
     CDS             complement(107..1174)
                     /locus_tag="PLQ68_03635"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HXK71776.1"
                     /translation="MKKFIINLIIALAVGCIFIVLMFTNQSADDAIRILKSIEYIYIL
                     FAFMCMVLLWVLGSLAIRIVKRGMIGRQKDPGGNFLSIIVHEFFCAVTPFSSGGQPAL
                     VYVLTKKGLDAGTAASIAIIRSFLYQVTELALSIFGFLLLRNYLLQHVSHFYAIFIPA
                     IIICSVIVGIFLAFLGKGKLANKTVSLVMLIIQKFMPKKHEKIEKSVNESLASFKEGV
                     RIFKTRPWFWVFSVLIQIAELLSIYMVPVFMLRAIEGSFDNALPLMVCTCICSIIMSY
                     VPTPGTTGGAEGFAVLLITPFFVSSPALPVILIWRLITYYSKILFGGIGFMVALRGKG
                     SDGKKVKAKTKDLISQADSMM"
     gene            complement(1171..1476)
                     /locus_tag="PLQ68_03640"
     CDS             complement(1171..1476)
                     /locus_tag="PLQ68_03640"
                     /inference="COORDINATES: protein motif:HMM:NF015721.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HXK71777.1"
                     /translation="MINNSGTDVAESIIVVKIAQTVGLFSTVRLTKKKRPIKITDNEK
                     RKTVEIFVDINYNIVIPQVTADIQRAIAKVLDKELDIVLDSIDITVEGVNIDKLENK"
     gene            complement(1482..2870)
                     /gene="asnS"
                     /locus_tag="PLQ68_03645"
     CDS             complement(1482..2870)
                     /gene="asnS"
                     /locus_tag="PLQ68_03645"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HXK71778.1"
                     /translation="MKISVQKLYDKPQDFDNKNVTVEGWVRTIRSSKDFGFIELNDGT
                     CFKNIQIVFESSVIENYSDVEKLPIASSISVTGSFVLTPNAKQSFEIKAQEINICGVS
                     SPDYPLQKKRHSFEYLRTVAHLRPRTNTFYAVFKVRSVLAQAIHTFFQDRGFVYVHTP
                     IITASDCEGAGEMFQVTTLDLDKIKGNKEEIDYSEDFFGKKTNLTVSGQLSVETYCMA
                     FKNVYTFGPTFRAENSNTARHASEFWMIEPEMAFADLNDDMSAAEDMIKYIINYVMER
                     CPYEMEFFDKFIEPGLFDKLKVVTDSKFAHVTYTEAVKILQDSKVKFDNLVEWGNDLQ
                     TEHERYLTEKHFKKPVFVTDYPKQIKAFYMKVNDDNNTVRAMDLLVPGVGEIIGGSQR
                     EERYDVLVSRMNEMGLDENDYWWYLDTRRYGTVKHAGFGLGFERMIMYMTGMGNIRDV
                     IPFPRVPKHAEF"
     gene            complement(3003..3227)
                     /locus_tag="PLQ68_03650"
     CDS             complement(3003..3227)
                     /locus_tag="PLQ68_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003358190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PC4/YdbC family ssDNA-binding protein"
                     /protein_id="HXK71779.1"
                     /translation="MPEIVYEIKENIGVISETARGWTREVNMVSWNGREPKLDIRDWA
                     PEKEKMGKGLTLSKEEAKKLRDLLNKIDLD"
     gene            3411..3890
                     /locus_tag="PLQ68_03655"
     CDS             3411..3890
                     /locus_tag="PLQ68_03655"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018596898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HXK71780.1"
                     /translation="MTRAIITMNDLSTMTLELHPSIAPITVENFTKLAKSGFYDGLVF
                     HRVIRNFMIQGGDPEGTGMGGPGHHIKGEFIANGVNNTIKHTRGVISMARSNDYDSAG
                     SQFFIMHKDSVHLDGQYAAFGKVIEGIETVDAIAQQKTDYSDRPVVEQKIKTIVIEE"
     gene            complement(3892..5061)
                     /gene="nagA"
                     /locus_tag="PLQ68_03660"
     CDS             complement(3892..5061)
                     /gene="nagA"
                     /locus_tag="PLQ68_03660"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HXK71781.1"
                     /translation="MSIIVYNGKIILKDHITEEGYVEINNGIIENIGKSKPEKYIIDR
                     ADEKIDAKGMYISPGFIDIHTHGGGGYEFGMSDELSYIIPCETHAKHGTTSLYPTISS
                     YTFDIMKNAIISYNKAKSITYKGATMRGLHFEGPYFAASQKGAQLEKYLRNPIESEYM
                     EILDMSDDIKRWSGACELEGMENFAKVLKSRNILAAIGHSNATYDEVVKALKWGFSLV
                     THLYSGCSTIKREKGYRIPGVVEAAYLLDELDVEIICDGHHLPDSLIQFVYKFKGPEK
                     TAVITDSLSFAGINAPNGTLLREDIVVEDGVAKLVDRSAFGGSIATYDMLIRTLIKAG
                     IPFLDIIKMSTITPARIMGIDKETGSLEKNKEADLIIFDEQINMHAVLVKGNRVL"
     gene            5157..5915
                     /locus_tag="PLQ68_03665"
     CDS             5157..5915
                     /locus_tag="PLQ68_03665"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HXK71782.1"
                     /translation="MKSAAQLYTLREFTQNESDYYKTLVKVKKMGYSTFQLSGAGPML
                     AQTVKSISDDLDIYVSATHIPHNMFINELDTVIENHHIMGCKYVGLGGMPKEYLENIN
                     KLNEFIKIYSDVSQKLKKEGLQFVYHNHNFEFFKFDGKTVMDILFENTPQSFFFELDT
                     YWVQSGGADPVEWIRKTAKRCEYIHFKDMGINSEFKTVFKPIGQGNLNWKAIIEACSY
                     SDVLICAVEQDTCEGSPFDALKASYDYLLSHGIR"
     gene            5925..7088
                     /locus_tag="PLQ68_03670"
     CDS             5925..7088
                     /locus_tag="PLQ68_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009589235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HXK71783.1"
                     /translation="MDKVRIGIIGYGNIANTHLAFFDKGEIPDSEITAIFDTDHSKVQ
                     KAKEKYDDKIKIFDNEEEFFQCGLFDAILICTPHYYHPHYAIKGFENNLHVLCEKPAG
                     VYTKQVKLMNDEYDKHPNLVFSMMYNQRTNPVYSRVREIIKSKEMGEMRRCVWIITNW
                     FRSQRYYDSGGWRATWGQEGGGVLLNQDPHQLDLWQWICSMPKRVRAFIGFGKYHNIE
                     VEDDVTAYVEYENNATGVFITSTAEAPGTNRFEISLDKGKIVIEDEKVRVYKLAVDLK
                     DYIKDTDKVFGAPDYEMSELEISKDNPQHKGILTNFVNAILHNEPLIAPGKEGILGLT
                     ISNAMYLSQWTDSWVDIPFDEDLFYSELQKRVSSSVFTKKEVKPVNADMSKSF"
     gene            complement(7085..>8059)
                     /locus_tag="PLQ68_03675"
     CDS             complement(7085..>8059)
                     /locus_tag="PLQ68_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006208464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HXK71784.1"
                     /translation="VNSGAEYLKIICYSYPMFAITTTLLIALRSVEKAKIGFYVSFST
                     LVTNIFLNYAMIFGEFGFKPMGIKGAAYATLISRALEMIFVVLYVFLSDKTINLKLKD
                     ILSTDISLFKAYLKKGVPVFLSNGIWGVAMAAQTSILGHMGQSSITANSIASTLFQIL
                     SVVAYGSANASSVLISKLIGEKKTDLIKPYAVTLQLLYIGIGLFTGALLFVFKDIVVS
                     WYIISEEARLLSLSFITILSVTVVGTSYEMSGLTGIVRGGGDTSFVLKNDFIFMWLIV
                     IPSSALAAFYFHASPIIVFMLLKSDQITKCIVAAIKINRYTWIKNLHS"
     assembly_gap    8060..8159
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            8408..8737
                     /locus_tag="PLQ68_03680"
     CDS             8408..8737
                     /locus_tag="PLQ68_03680"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HXK71785.1"
                     /translation="MINFSYIGQRIRTKRKELKMTQQELSEMASISPAYISRIEGGQQ
                     NITLDTIEKIAKSIDEDPIYLISGVKDDDVNYVKKLFNRAPAQFLYLFELALVELEEK
                     GILNIKP"
     gene            complement(8720..10447)
                     /gene="hflX"
                     /locus_tag="PLQ68_03685"
     CDS             complement(8720..10447)
                     /gene="hflX"
                     /locus_tag="PLQ68_03685"
                     /inference="COORDINATES: protein motif:HMM:TIGR03156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HXK71786.1"
                     /translation="MDKLVSGNTEGLKKSMLDMLLSIYEDKFDSDLYCSLDTITKICY
                     VTYETKREVAVYIDKKNTVTEVAVGDSSLVKSDYEPSKGIRLIHTHPNGSAILSHVDI
                     QTLKNTALSAMAAVSIKNDGTMDMMYCAVVDESDEPVLFGPYMDIEQTSYFMKILKEN
                     YYKKTDELIDIQEQKERVIAVGLNSTYSKSLIEELKSLIETAGGKCVATVVQNKSEPD
                     SAYYIGKGKLRELLHLCSQYQADTVVFDDELTYSQQNNIESELNVKVLDRTSVILDIF
                     AKRAFTNEGKLQVELAMLSYMYPRLTGKGQMLSRLGGGIGTRGPGESKLETDRRHIRR
                     RMTYLKEQLDKVDKRRSLTRDNKTRKSFFTFALAGYTNAGKSTLLNLLTESSVVAEDM
                     LFATLDPTIRSLKLPSNSQVMLIDTVGFIAKLPVKLIEAFKSTLDEIRYADCIIHVID
                     GSNPDASRQMDEVYSILKSIGADTVPVIEAVNKTDLVKDPILNASTCPRAYISCANGQ
                     GIDDLLSLMDKASKNKFINMKLKVDFENGKLLSFIHSNAKVISKTETEEGIDIEFEIA
                     SELVSRLNI"
     gene            complement(10447..>11664)
                     /locus_tag="PLQ68_03690"
     CDS             complement(10447..>11664)
                     /locus_tag="PLQ68_03690"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HXK71787.1"
                     /translation="SYKVADSKGMGFRGVYEGNMGYSYTEKIAEDSIDILLREAMQNA
                     KINDSKEQDIIYEGSKEYEEIDNYFPKLDEISVETKIEFAKEMEKIAKSLDSRIKSCP
                     YCFFGSGSGNTGLVNTKGLDLKDRGNSIYCFIEALAQQADDMSDGFAFCIENDFSQFD
                     AHKIAKEAVEKAVSYLGAKPIKSGAYKAILLNTVSSDILSAISSCFSARAVQKGISFL
                     KGKLGTDVASESVTIVDDPFMPGQSGTSSFDGEGVATRYKEVISNGKLMTYLHNMKTA
                     RIDKTESTGNASRSYKSNIGVSPTNLYFRKSEISFEQLEQKMSDGIVITEVSALHSGF
                     NAISGDFSLPAKGYTVKEGKKDKYFRQITVSGNYFELLKNVISVSDDLTFGTNGNIGS
                     PSLLISNINVAGE"
     assembly_gap    11665..11764
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<11765..13008)
                     /locus_tag="PLQ68_03695"
     CDS             complement(<11765..13008)
                     /locus_tag="PLQ68_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006307610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HXK71788.1"
                     /translation="MLDKNTIFDVLTAAMSKGADFAEVFLDDTVASNINYVDKQVEST
                     QSGRDFGIGIRVIRGLNCVYAYTNSFIKEEMINTALKAAAALEGVKKDITINLCKSDV
                     FNRNPIKIMPDLVDKSEKLEYMKQAYSTIMGYDECIKQASITYLDRIQNILVANTEGL
                     FKTDKRVYTRMAISAVAEYNNEMQTGFAGPGAHKGFEFVKEMDIVDISRNAAETAKKM
                     AYAGYCKAGAYPVVIDNGFGGVIFHEACGHSLEATSVAKGNSVFSGKLGEKIAADCVT
                     AIDDATLPNQWGTTNMDDEGGETRKNILIENGILKGYLIDKLNGKRMGMESSGSGRRQ
                     NYRYAPTSRMSNTFIDNGSSKKEEIIQSTDFGLYAKQMGGGSVNPGTGEFNFSVQEGY
                     MIRNGKIAEPVRGATLIGKGSE"
     gene            13190..14182
                     /locus_tag="PLQ68_03700"
     CDS             13190..14182
                     /locus_tag="PLQ68_03700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK71789.1"
                     /translation="MKKIFIALFTLIILSVLPIPSSAQPVGIIHEPGEIYQGDTLYFV
                     DVNLTSNTGTTDTFKLALLKGDYLASLPLSQLKSDLSNNWTKLIPEIQTQRNTLIYLK
                     EGSDIIKSVTSEDYSISQIVLNAARQYYGINNAQISSTAYLDNNIVLSNNVSNEDGHI
                     MYDAASNTMQLHYTYDISEQTIFINENGVSNEYLLKRYNMVVYRVICMAVDLTYSINQ
                     PTPTPTSTPTPTPTLTPTPTTTLSPEPSVTLSPTSTISPSPSPTASSTQPVNSSQPSP
                     SPELSPTSSPSPSPRVEVINREDKRYDDIWTFVILQALIIIIIIIIVLSRKKRH"
     gene            complement(14183..16042)
                     /locus_tag="PLQ68_03705"
     CDS             complement(14183..16042)
                     /locus_tag="PLQ68_03705"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HXK71790.1"
                     /translation="MAIITLNNVNKSYGGFDVLKDISFEIHANEKVALIGKNGSGKTT
                     IFNIIADNMDYDSGSVYVEKGLRIGYLRQIYSDFGSKTSKTVIEEAFEHIKQVEHQMS
                     ELRKLMTIQPDNEEHIRNYSILNDRFEFYEGYSMNEKFNKVTKGLGIPDAVIENQAAK
                     LSGGELTMIMLAKVLLMNPDILLLDEPTNHLDANACEWLEKYIIEYKGAVLYISHDRY
                     FIDNTAMKVIELYDGKVESYKGNYSAYQVQKIEAEERDLKLYERQEREINRLRQTALK
                     MRNYGTEIAIKRAKNLERRMERLDIYDKPVTEKELRLAFLEEEKVAKEIFFVKSIEKS
                     FENRVLFSDVSFIMRSGDKVALIGPNGAGKSTLIKIVTGQIEADSGIVKRPKSVKYAY
                     LEQHVTFPNEDETVLELVCEQLSMTLQSARNVLGKFLFSDDDVYKKVGSLSGGEKSRL
                     RLLLEMQDGVNLLILDEPTNHLDIPAREELEDALMMFSGSMIFISHDRHFINKFAKRI
                     FEIENGMFNVYEGDYDYYLAKKEALVQQDKISRTFVKKPFDDKKEKRKTDLKKRTCEQ
                     QIKTLENELNEVISQMNNNSSDYEKLMELTEQKKQLNSKLDELYGIWLETESD"
     gene            complement(16128..16751)
                     /locus_tag="PLQ68_03710"
     CDS             complement(16128..16751)
                     /locus_tag="PLQ68_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008720979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HXK71791.1"
                     /translation="MFNKKDFDYYQKFVELVEYNCRAADLLCEIVYNYDLKKAQTMLK
                     SMHDVEHEADMQKHTLVNKLIKEFITPIDREDIINLSQEIDDVTDNIEDVLIKIYMYN
                     IKTIKKGALDFTDIIKRCCEALRKTFIEFRNFSRSKTIKGLIIEVNDLEEEGDKLFVE
                     SMNELFASDADAVEKLVWKSIFENLEKCCDSCEHVANIVETTIMKNS"
     gene            complement(16762..17814)
                     /locus_tag="PLQ68_03715"
     CDS             complement(16762..17814)
                     /locus_tag="PLQ68_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016208945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HXK71792.1"
                     /translation="MQIEFGTYLQSLGSNPLLLVTTLLTMAVILVNGWTDAPNAIATA
                     VSTRSISPKIAVLMAAVFNFLGVLVMTLINSTVAETLFNMVDFGDDYKTALIALCAGL
                     CAIVIWAVTAWIFGIPTSESHALVAGIAGAGIAVSNSFSSINGPEIVKVLYGLLFSTF
                     LGFLMGFLTVRAVEFIFKRVNKTKTGRLFQGAQIGGAALMAFMHGAQDGQKFMGIFIL
                     GIYLSQGMSSATNLIFPMWMMILCSAVMGIGTSIGGYRIIKTVGMNMVNLEKYQGFSA
                     DLAAAGCLLISSLTGIPVSTTHTKTTAIMGVGAAKRIRNVKWSVVKEMVLTWIFTFPG
                     CGLIGYLMAKLFLAVF"
     gene            complement(17859..19121)
                     /locus_tag="PLQ68_03720"
     CDS             complement(17859..19121)
                     /locus_tag="PLQ68_03720"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family metallopeptidase"
                     /protein_id="HXK71793.1"
                     /translation="MDIRGKRIYETLKKMNFIRLSTTDGEKKGADIISEEIKQTGLEP
                     VYEYFKVPCYEIVKASLEVLEPKRMSFNIKGYGYSGNSAKDGIIADFKYVETADEIDL
                     IDAKGKIVLVSSLGYETYERLVKAGVAGFITPSGDYFDDPKKTDLDERMLRKGHIQNG
                     QIPGVCIRMKDAVNLMKSEPKKIQLVLEQKEDEGDSKNIITEVKGTKYPDEVIVYTAH
                     YDSVVFSKGMYDNAAGSAILLELLRYFVKNPPLRTVRFIWCGSEERGLFGSKNYVKEN
                     ADKLDNIKLCINVDLAGPVIGRDTAIVLAEEKLCHMIEYMYKEIGHPMSLRHDIYSSD
                     SIPFADKGIPAVNFVRYGAPGAVSCHTRNDVIDPISAKSLEYTASFVAQFSDRIVNAY
                     MFPVAREIPEELVKKIDKYLLKKQLKTD"
     gene            complement(19146..>19388)
                     /locus_tag="PLQ68_03725"
     CDS             complement(19146..>19388)
                     /locus_tag="PLQ68_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HXK71794.1"
                     /translation="MEDMMKKIPNDIIAMGNVDPAGQIKNGTVDSIREETLKIMKKCC
                     KYDNFIISTGCDVPPMAPWENINAFFDAVNEYYNKA"
     assembly_gap    19389..19488
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<19489..19999)
                     /locus_tag="PLQ68_03730"
     CDS             complement(<19489..19999)
                     /locus_tag="PLQ68_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HXK71795.1"
                     /translation="MKNSKVKKAMPVLSFPSIQLLDITVKQLISDYELQAQGMKAVAD
                     KTDSAASVSLMDLSVEAECFGANIIASDDEVPTVTGHVVTTMEDAVNLVVPEVGCKRT
                     KIYIEAIRKAKQLITDRPVLAGVIGPFSLAGRLLDVTEAMVLCYDEPEIVHEVLKKVT
                     SFITEYIKAY"
     gene            complement(20049..20393)
                     /locus_tag="PLQ68_03735"
     CDS             complement(20049..20393)
                     /locus_tag="PLQ68_03735"
                     /inference="COORDINATES: protein motif:HMM:NF040311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6054 family protein"
                     /protein_id="HXK71796.1"
                     /translation="MSKFEKVVKGSFYDISEKLHNDISNSAVSINLVDEARKEVNGCE
                     MLVRVYDKYFMRNSSRASLTVVMISNKDNDEIYVSAIGSGGGTGVFLRLDWGAENNLV
                     YLVEDSLANMGY"
     gene            complement(20478..20551)
                     /locus_tag="PLQ68_03740"
     tRNA            complement(20478..20551)
                     /locus_tag="PLQ68_03740"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20517..20519),aa:Gly,seq:ccc)
     gene            complement(20600..21280)
                     /gene="pnuC"
                     /locus_tag="PLQ68_03745"
     CDS             complement(20600..21280)
                     /gene="pnuC"
                     /locus_tag="PLQ68_03745"
                     /inference="COORDINATES: protein motif:HMM:TIGR01528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HXK71797.1"
                     /translation="MFRNWTKPDIIFYGASMVLIITAGIIFDAALISLVISIVGITAG
                     IMNMKADKYCYLFYIVQITLYAFVSWRNRFIGEAVLAAFYLLPLYIYSSFRWLGKKSV
                     KTDFQIFRITKKLIVILAIACVVVTVGYGYILSLLKSNLPYINALGTFTSVAAGFLTS
                     RRIKEQWYFWIAYSLIFSAIWISTLSADTTQITLVLQNIPYLAINIVGLIKWNKLYKE
                     DEKNKALT"
     gene            complement(21294..22079)
                     /locus_tag="PLQ68_03750"
     CDS             complement(21294..22079)
                     /locus_tag="PLQ68_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015357909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HXK71798.1"
                     /translation="MEVLASFGQAVSSIPTNIWIFILVGFFAQTIDGTLGMAYGVSCN
                     SFLLTFGVPPALASASVHMAEVFTTLVSGISHWKLKNINKGIFFKLLIPGIIGGAVGA
                     YLLTSFDGNTVKPYINGYLVVMGIIIIVKIFRKQKPKNLSGWIYPLGLSGGLLDAIGG
                     GGWGPVVTSTLIASGVEPRYTIGTVNAVEFFVTLAESIMFISFLGLEQSMYAIIGLII
                     GGVIAAPFGAFLVKRLPMKPLMALVGALIIFLNIKSLIGYIIG"
     gene            complement(22084..23874)
                     /locus_tag="PLQ68_03755"
     CDS             complement(22084..23874)
                     /locus_tag="PLQ68_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013389252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HXK71799.1"
                     /translation="MTKKNDEHMNIVIVGHVDHGKSTIIGRLLADTNSLPEGKLEQIK
                     QLCRNNAKPFEYAFLLDALKDERSQGITIDTARSFFKTEKRKYIIIDAPGHIEFLKNM
                     ITGASRADAALLVIDAAEGVKENSRRHGYMLSMLGIKQVCVLVNKMDNVKYKKEVYDR
                     IVNEYNEFLNKIGIQSQGFIPVSGMEGDNIAKRSINMPWYEEDTVLTMLDKFTASKPL
                     DSLPFRMPVQDVYKFTNDADDRRIIAGTVESGTINNGDEVVFYPSGKKTHVNKIEPMS
                     EYDTATKASAGYATGFTMTEQIYVKRGELCFKSDQTPPLMGKHMKVSLFWLGKKPMVK
                     DKRYFLKTGTMKAECYLENILSVIDASTLIKTEKDQIDKHDVADCIISTSKLCAFDNS
                     ALLSSTGRFVIVDEYEISGGGIFTETVDNLHRYEKAKVMLREGKWEHGYVTLPERNER
                     FSQNAALIILTGTKDTNKKLFAKALERSLFDEGKNVYYLGIGSVIYGLNADINPPGEK
                     ENRSGENIRRLAEAANILLDTGLILIVTASDLTKYDVETIKLVTKNDNTLVFWLGDEC
                     TTDIECDMVLPNDEDAIEIIRKKVEQVAVR"
     gene            complement(23876..24757)
                     /gene="cysD"
                     /locus_tag="PLQ68_03760"
     CDS             complement(23876..24757)
                     /gene="cysD"
                     /locus_tag="PLQ68_03760"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014313517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009336 - sulfate adenylyltransferase
                     complex (ATP) [Evidence IEA];
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA];
                     GO_process: GO:0070814 - hydrogen sulfide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HXK71800.1"
                     /translation="MDHLDRLEARSIHIIREAYNSFKNLCMLWSIGKDSTVLLCLARK
                     AFYGHVPFPLVHIDTHYKIPQMIEYRDRLAIEWNLDMIYGENRKALEEKRTFPDGNCT
                     RLECCRNLKTEALAHTLAGDWPRYRMDHNLKKYVIDKNTEPYTGVIVGVRADEEGSRS
                     KERYFSPRDTHNDWDISDQPPEFWGQYKTDFAPGTHLRIHPLLDWTELDIWEYIKREN
                     VPVVSLYFDQGEKKRYRSLGCYPCTKPVDSEAKNLDDIIEELRTGKFANIAERSGRDQ
                     DKEDGGGLEELRKNGYM"
     gene            complement(24759..25334)
                     /gene="cysC"
                     /locus_tag="PLQ68_03765"
     CDS             complement(24759..25334)
                     /gene="cysC"
                     /locus_tag="PLQ68_03765"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012574033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HXK71801.1"
                     /translation="MENIVWHQQLDKKYRQLKTNQKGSVIWLTGLSGSGKSTIGRIIE
                     KKLIDKGIMAYLLDGDNIRHGLCSDLGFSEEDRSENIRRIAEVAALFEDSGVTVLVSA
                     ITPFEKMRNLARIKSKKFILVYVKADIDTCVKRDPKGLYKKALNGEILGYTGIDSPFE
                     EPIEYDYLLDTEKFSIDECADKLMEYLLGVI"
     gene            25472..26188
                     /locus_tag="PLQ68_03770"
     CDS             25472..26188
                     /locus_tag="PLQ68_03770"
                     /inference="COORDINATES: protein motif:HMM:NF024860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HXK71802.1"
                     /translation="MLITVIGRGHSGTRAISQTLKDSGVFMGEPLNKSYDLVPAGDMY
                     EACRVFAKYVTYLGDMKWDFSKVMSMDPDPAFVKLIESYLSSVLSSKSQNKGWKIPET
                     TLVYPWIMKMFPEAFYIHWVRDPRDCIIGGHMTDDLNDFGISYDKSEDIRKNRAISWF
                     YQREIVRHSPVPEKIASVRFEDMVFRQDETLQKLEKFLNIPLVKIPMRTDSVGRYLTD
                     DQINMFDFFKNDMTECGYEF"
     gene            complement(26176..28086)
                     /locus_tag="PLQ68_03775"
     CDS             complement(26176..28086)
                     /locus_tag="PLQ68_03775"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HXK71803.1"
                     /translation="MLKKPELFMLGVDGGVTSYVLEQIEKGKLPNFKKMIDNGCMLSD
                     CRPVYPSISATCWATIQTGASPHMHQITDENILLEGAYPNEFVTAYHGDYLMAERFWE
                     AAAKKGKKSLIADFCVSGPAKSDLVHQIGGIGSVADQKAIKTEQTMRMQKEISEQLYL
                     FDTDVLSVFPKIPEQTMAPSGKWQPQIKLTQSRYEKSEKGYFKLYVEKKNDLENPNNI
                     EPFYWIMSIYDEGIKIAFNEADMENGQYYEIPMGNWSEVIKRTLKDDLGEGSYRFRIK
                     AMKANDKGGFFVFITSSVNLFNALSPYEFANRVNEIDEICSSNSYGHFLTRDGYIDTA
                     FELIAFKNEWFKKIILDSMENDPVEIMAFVQGYTDTVNHVFRGLYEGVEKSNEQQVEI
                     ARYVYEKAYDMLDDFIGWLYENVIGEDTVFIIIGDHGAVGFNRTVLVHDFFEKEGFLK
                     YIPHPKNEHVYWRDRKIDWKNTKAFPVGCGNIYINLKGREPEGIVDPEDYDKTVNEII
                     KILHKYHWNEKDECYLAFAVEKKQAGFVGLGTERVGDVIYGLSGNSTGGYIGGVHACQ
                     IPSAKTKTGDIRCLCMFSGKNIKKGELVDRPANLEDIAPTLCYLLKYPQPASATGGIL
                     FQIFKDDMYQNS"
     gene            28276..29073
                     /locus_tag="PLQ68_03780"
     CDS             28276..29073
                     /locus_tag="PLQ68_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HXK71804.1"
                     /translation="MIKLGVNTVLFKHYTFAQAAEAIAKCGYDGVEISAIPGMCEHLQ
                     PENWRSQKKEIKAVLEATGLEILASEVASRDKKRAKKAFEAAEYFNIPVINVGPGGKS
                     GVEKDFLESVKSLSELSKMAEKYGVTLCCKAHVGASIHDTATTLRAMDMIESPNFGVD
                     MDPSHIYRANELPEEALAKVLTRMKHIHIRDCKGRGPSPGLPALQACGKGDVNLFGYI
                     SEMVKAGYNGPVDLEVIGPEQDMVEATRIAAESYGYMNACLKVLKAR"
     gene            29138..30517
                     /locus_tag="PLQ68_03785"
     CDS             29138..30517
                     /locus_tag="PLQ68_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010151972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HXK71805.1"
                     /translation="MAKKPNLLFFGIDSLRRDHMSLYGYDRLTTPNIDKYLKDGTVFN
                     NCFSPHIPTTPGYANMMTGFDCFGTDIVALRHKGEIAPNVKTLAEILSENGYNTTNVG
                     FKNVSGRGFDNYIEFEGWKAEEGDGRAHKAENLNNVAIPELERLSKEDKPFFLFLRHM
                     DPHSPYLPPAPFDKMFYQADAFDPDNHTLDPVYSFKPFCDYFVTWFPKGCTDKDYIIA
                     QYDSAIAYMDSCIQAILQKVKDLGLENDTLVVFTSDHGETLYDHDCYFDHHSLYDPNL
                     IVPLALSFPGHVPQGKRVDDSCRLQDVTPTVLDILGIDAKIDFDGRSLMPLANGIPLP
                     KQNEFYITECTWMRKHGWRTPKWKYIKALEPDFHYKPEVELYDLENDPLEYTNIAELR
                     KDIVMMLDKKMNDFIKTREKQVKRENPMYTNLNWHGLNLGRGFESSDEAYNSMHIGSP
                     GAAAKLQQK"
     gene            30531..31541
                     /locus_tag="PLQ68_03790"
     CDS             30531..31541
                     /locus_tag="PLQ68_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HXK71806.1"
                     /translation="MLKAAVIGMGPIGNNHATAYKNCKLAELVSVCDIRKDRADAAKE
                     KYKVAAYYDAKEMLDKEKPDIVSVATGGFEYSSDHFIPTMQALNAGCHVLTEKPISNN
                     LQHGQQMVDLAKELNRCFAVDLNHRFTPAARAAKKWQTDGLIGDLLFLNMALWIGRFQ
                     PQFDTEYYHLKALNPHSCDILRYFGGDVEQVHCYAMKAPGRQIWSTASINMKFKNGAV
                     GHLTSSYDIERGHPMERCEVAGTKGRLIFEDMWREATLYPAGNYEKRVYTNPVFGGFR
                     DFYDTFEDRIHTFVREISENVKPENIDGSGKDGLESSRIIHAAIQSLKTNKPVMVQSV
                     TE"
     gene            31552..>31754
                     /locus_tag="PLQ68_03795"
     CDS             31552..>31754
                     /locus_tag="PLQ68_03795"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HXK71807.1"
                     /translation="MAIKIGIVGMGGIGNTHAKCYKADSLAQLVAVCDVVKEKADKAA
                     SEYNVEAFYSLKDMIKAHPELDLV"
     assembly_gap    31755..31854
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <31855..32520
                     /locus_tag="PLQ68_03800"
     CDS             <31855..32520
                     /locus_tag="PLQ68_03800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK71808.1"
                     /translation="PVYILHKVGFNGSDMSYKAPANGGPMKRFEGPYSHAYAFLSHPF
                     SLMRSFGGDITHVQAFFDKPGVRHSANDLMWSINGINVKYQNGSIGYLLSQRGDAIFG
                     LGGWWSFEMAGTKGTFCIENCVEKLYYWDGAKPSSMGTMEPEVFDFGTKDFGITFNNR
                     LHAFLEDMTNKIPPEHIRSSGRDALATLEYTVAAIQSFEQGGELIRPRALPPLHGTPG
                     FVV"
     gene            32520..>33130
                     /locus_tag="PLQ68_03805"
     CDS             32520..>33130
                     /locus_tag="PLQ68_03805"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HXK71809.1"
                     /translation="MKKPNILLFLADQHRYDCVGYKNSDIQTPNIDRLARQGVVFNNA
                     FCPTPVCAPARQSFMSAMRAESIGALWNFSFINTTTIQPDDWNIVRNLKDAGYKNLFI
                     GKWDVSHTSEYDYGFDTVHSSSEYNAFIKQNYPNVEYTNKWFGEKNPIPYKHSRPKYY
                     ADKACLELNKMKNSDTPWFMWIDGVDPHLPCRPSDQFYDMYKPS"
BASE COUNT        10385 a         6395 c         5070 g        10880 t
ORIGIN      
        1 tattaagtat ttcttttctt cttggtgatg atgaagccag aattaaacgc gtcatttatc
       61 ctcctattat tgacatttta taccattttt aaaattaatg caataattac atcatactat
      121 ctgcttgact aattaaatct ttagtttttg cttttacctt tttcccgtct gatcctttac
      181 ccctaagagc aaccatgaaa cctattcctc caaacaaaat tttagaatag taagttatta
      241 aacgccatat taaaataaca ggtaaagctg gtgatgaaac gaagaatggg gttatcagca
      301 gcactgcaaa tccttctgca cctccagtag tacctggagt tggaacatag gacattataa
      361 ttgagcaaat acaagtacaa accattagag gaagagcatt atcaaaactt ccttcaattg
      421 ctcttaacat aaagactggg accatataga tagataaaag ttcagcaatc tgtataagca
      481 ctgaaaacac ccaaaaccat ggtcttgtct tgaatattct aacaccttct ttaaaactgg
      541 caagagattc attaactgat ttttcaatct tttcatgttt tttaggcata aacttttgta
      601 ttattaacat aaccagagaa acagttttat tagcaagttt gccttttcct agaaaggcta
      661 gaaatatacc tactataaca gagcatataa ttattgcagg aataaaaatc gcataaaaat
      721 gtgatacatg ttgtaaaaga taattcctta atagtaaaaa accaaaaata gataaagcaa
      781 gttctgttac ctgatataaa aatgatcgta taattgcaat tgaagcagct gtaccagcat
      841 caagaccttt cttcgtgagt acatagacca gtgcaggctg tcctccagaa gaaaatggtg
      901 ttactgcaca gaaaaattca tgtacaatta ttgaaagaaa attcccgcca ggatcttttt
      961 gccttccaat catgcccctt ttaactatcc ttatagccaa cgaacctagt acccacaaca
     1021 aaaccatgca cataaatgca aataatatat atatgtattc aatagatttt aatatgcgta
     1081 tcgcatcatc tgctgattgg ttagtaaaca ttagaacaat aaatatacat cctacagcaa
     1141 gtgcgataat cagattaatt ataaatttct tcatttgttc tccagcttat cgatatttac
     1201 accttcaaca gtaatatcga tagaatccag gactatatct agttctttat caagtacttt
     1261 tgctatagcc ctctgtatat cagcggtgac ctgaggaata acaatgttgt aattaatatc
     1321 aacaaaaatt tcgacagttt tccttttttc attatcagtt atctttatag gtcttttctt
     1381 ctttgtgagt cttacagtac taaataaccc aactgtctgg gctattttaa ccacaataat
     1441 gctttcagct acatcagtac cggaattatt aatcatttct tttaaaattc tgcatgttta
     1501 ggaactcttg ggaaaggtat cacatcacgg atattgccca ttcctgtcat gtacattatc
     1561 atcctttcaa aaccaagtcc gaaccctgcg tgttttactg ttccatatct tctggtatca
     1621 agataccacc agtaatcatt ttcatctaga cccatctcgt tcattctact tacaaggaca
     1681 tcatatcttt cttctctctg gcttccacct attatctctc cgactcccgg aaccagaaga
     1741 tccattgctc ttactgtatt attatcatca ttaactttca tatagaaggc tttaatctgt
     1801 ttgggataat ctgtaacgaa gacaggtttc ttaaaatgtt tttcagtaag atatctttca
     1861 tgttctgtct gaagatcatt tccccattca actaagttgt caaactttac tttgctgtct
     1921 tgtaatattt ttacagcttc agtatatgta acatgtgcaa atttggaatc agttacaact
     1981 ttcagtttat caaacaaacc aggctcaata aatttatcga aaaactccat ctcatatgga
     2041 catctttcca taacataatt aataatatat ttaatcatgt cttctgcagc tgacatgtca
     2101 tcattcagat ctgcaaatgc catctccggt tctatcatcc aaaattccga tgcgtgtctt
     2161 gctgtgtttg aattttcagc tctgaaagtc ggtccgaaag tataaacatt cttgaatgcc
     2221 atacaatacg tttccactga aagctggccg cttacggtaa ggtttgtctt ctttccgaaa
     2281 aaatcttctg agtaatctat ttcctcttta ttacctttaa ttttatcaag atccaatgta
     2341 gtgacctgga acatttctcc tgcaccttca cagtcacttg cagttattat cggagtatgg
     2401 acatacacaa agcctctgtc ttgaaaaaaa gtgtgaattg cttgtgcaag caccgaacgg
     2461 actttaaata ctgcataaaa tgtattagtt cttggacgaa gatgagcaac tgttctaaga
     2521 tactcaaaag agtgcctttt cttctgcaaa ggataatctg gtgaacttac tccgcatata
     2581 ttgatttcct gtgcttttat ctcaaaggac tgttttgcat ttggagtaag tacaaaactg
     2641 ccggttactg atatgctaga tgctataggc agtttttcaa catcagaata gttttctatt
     2701 acactgcttt caaaaacaat ctgaatattt ttaaagcagg taccatcatt aagttcgata
     2761 aaaccaaaat ccttagaact tcttatagtc ctcacccatc cttcaactgt tacattttta
     2821 ttatcgaaat cctgaggctt gtcgtatagt ttttgtacac tgattttcat tattatataa
     2881 tccttccatt taatcactca aaaggtatta taccaaaggc attatacatt ttaaataaaa
     2941 aaaacaataa atgatacaag gccgctaaag cagccttgcc aaaagaatat tatttaagct
     3001 ctctagtcaa gatcaatttt attgagaaga tctctaagct tctttgcctc ttcctttgat
     3061 aaggtcagtc cttttcccat tttttctttt tctggtgccc agtcccttat atcaagttta
     3121 ggctctcttc cgttccagct aaccatattg acttctcttg tccagcctct tgcagtttct
     3181 gatattacgc caatattttc tttaatttcg taaacaatct ctggcattag tattctcctt
     3241 ttaaatattt acatctattg taacagtaca tatgacattt ttcaatatac cgttatattt
     3301 attactgtat atatacttaa ttttttatta atataattat ataaatatta aaaagagagt
     3361 atttgtgtta aaatgcattc atgaattaat atgtttgaaa ggaaaatcaa atgacaagag
     3421 caataataac aatgaacgac ttaagtacaa tgactttgga actacacccc agcatagcac
     3481 ctattactgt ggaaaacttt acaaaactgg caaagagcgg attctatgac ggccttgtat
     3541 ttcaccgggt tattagaaac tttatgattc aaggtggaga tcccgaagga accggaatgg
     3601 gcggaccggg acatcatata aaaggagaat ttattgctaa tggtgtaaat aatacaatta
     3661 agcatacaag aggagtaatc tcaatggcaa gatcaaatga ttacgattca gcaggatctc
     3721 agttctttat aatgcacaaa gattccgttc accttgatgg tcagtatgcg gctttcggaa
     3781 aagtaattga gggtatcgaa acagtggacg ccattgcaca acagaaaact gattacagtg
     3841 accgccctgt tgtagagcag aaaattaaaa ctatagttat tgaggaataa tttacaatac
     3901 gcgattacct tttacaagta ctgcgtgcat atttatctgt tcatcaaaaa taattaaatc
     3961 ggcctcttta ttcttttcta aagagccggt ttctttatct atacccatta ttcttgcagg
     4021 tgtaatagta gacatcttaa ttatatctaa aaacggtata cccgctttaa ttaatgtccg
     4081 tataagcata tcatatgttg ctatggaacc tccaaaagca gatctatcga ctagtttagc
     4141 gaccccgtct tctaccacaa tatcttctct taaaagggtt ccatttggtg catttatacc
     4201 ggcaaacgaa agactgtctg ttattacagc agttttttct ggtcctttga atttatatac
     4261 aaactgtata aggctgtcag gcagatgatg accatcacat ataatttcaa catccagttc
     4321 atcaagaaga taagcagcct cgacaacacc tggtattcga taacctttct cccttttaat
     4381 ggttgaacaa ccggaataaa ggtgagttac aaggctgaac ccccacttaa gcgcttttac
     4441 tacttcatca tatgtcgcat tgctatgacc aatcgctgct aaaatattcc ttgatttcag
     4501 aaccttggca aaattttcca taccctccag ttcacaagct ccggaccatc ttttaatgtc
     4561 atctgacata tccaatattt ccatatattc tgattcaata ggatttctca gatatttttc
     4621 cagttgagct cctttctgac ttgctgcgaa ataaggacct tcaaagtgaa ggcctctcat
     4681 agtagctccc ttgtaagtaa tactcttagc cttattataa ctgataatag cattcttcat
     4741 aatatcaaaa gtatatgatg aaattgtcgg atacagactt gtagtaccgt gttttgcgtg
     4801 tgtttcacat ggaataatat atgatagctc atcagacata ccaaattcgt aacctccacc
     4861 tccatgagtg tggatatcta taaaacctgg tgatatatac attcctttag cgtcaatctt
     4921 ttcatctgct ctgtctataa tatatttttc cggtttactt tttccaatgt tttcaataat
     4981 tccgttgttt atctcaacat atccttcttc agtaatatga tctttcaata ttatttttcc
     5041 gttatacaca attatactca ttagccacac cttccttctt ccattatatt atctaatggt
     5101 tgaataaaca acatcatcag gtataattaa gtaagagaaa aaggaagggt gattacatga
     5161 aatcagcagc tcaactttat acattaagag aatttactca gaatgaaagt gactattaca
     5221 aaacattagt caaagtaaaa aagatgggat attcaacttt tcaattatcc ggagcaggac
     5281 ctatgcttgc acaaacagta aagagtataa gtgacgattt ggacatttat gtgtcagcta
     5341 cacatatacc tcataacatg tttatcaatg aactggatac agtaatagaa aatcatcata
     5401 ttatgggatg taagtatgta ggtttaggcg gtatgccaaa agaatatctt gaaaacataa
     5461 ataagctaaa tgagtttatt aagatatatt cagatgtgtc tcaaaagctt aaaaaagaag
     5521 gattacagtt tgtttatcat aatcataatt ttgaattttt taagtttgat ggaaaaactg
     5581 taatggatat tctttttgaa aatacaccac agagtttctt ttttgaactg gacacatact
     5641 gggttcagtc aggaggagct gaccctgttg aatggataag aaaaacagca aaaagatgtg
     5701 aatatatcca ttttaaagat atggggataa atagcgaatt taaaacagtg tttaaaccaa
     5761 ttggccaagg aaacttaaac tggaaggcta tcatagaagc atgtagttat tccgatgtat
     5821 taatttgtgc agtagaacag gatacctgtg aaggttcgcc ttttgacgca ttaaaagctt
     5881 catatgacta tttgctttct catggtataa gataggggta aattatggat aaagtcagaa
     5941 taggaataat tggatatggt aatatagcaa acactcatct tgcgtttttt gataaaggtg
     6001 agatacctga ttcagaaata acggcaattt ttgatactga tcattcaaag gttcaaaaag
     6061 cgaaagagaa atatgatgac aaaataaaaa tctttgataa tgaagaagaa ttctttcaat
     6121 gcgggttatt tgatgcaatc cttatttgca cacctcacta ttatcatcct cattatgcaa
     6181 tcaaaggttt tgaaaacaat cttcatgttt tatgtgaaaa acctgcaggg gtatatacaa
     6241 aacaagtcaa acttatgaat gatgaatacg ataagcatcc aaatctcgtc ttttcaatga
     6301 tgtacaatca aagaactaat ccggtatatt ccagggtaag agagattata aagagcaaag
     6361 aaatgggaga aatgagaagg tgcgtttgga ttataaccaa ctggttccgt tcccaaagat
     6421 attatgactc tggaggctgg cgtgcaacat gggggcaaga aggtggaggc gtattattaa
     6481 accaagaccc gcatcagtta gatttatggc agtggatctg ctcaatgcct aaaagagtaa
     6541 gagcttttat aggatttggg aaatatcata atatagaggt tgaagatgat gttacagctt
     6601 atgttgaata tgagaataat gcaacaggag tgtttataac atcaacagca gaagcacctg
     6661 gtactaacag atttgaaatt tctttggata aaggaaaaat tgtaattgaa gatgaaaaag
     6721 ttcgtgtata taagctggca gtcgatctta aagactatat taaagatact gacaaagtct
     6781 ttggcgcacc agattatgaa atgtctgagt tagaaatcag taaagacaat cctcagcaca
     6841 aaggaatact taccaacttc gtaaatgcca tactgcataa tgaaccatta atagctcctg
     6901 gaaaggaagg catattaggg ctgacaatat ccaatgctat gtatttatca cagtggactg
     6961 acagctgggt agacattcct tttgatgaag atttatttta ttcggaatta caaaaaagag
     7021 tatcttcttc agtatttaca aaaaaagaag taaaacctgt taatgctgat atgtcaaaat
     7081 cattttaaga gtgtaaattt tttatccatg tatatctgtt aatttttata gcggctacga
     7141 tgcacttagt tatctggtcg ctttttaata acataaaaac aattatcgga gatgcatgaa
     7201 aataaaaggc tgctaaagct gatgatggaa ttacaatcaa ccacataaaa ataaaatcat
     7261 tcttcaaaac aaatgatgta tctcccccgc ctcttacaat accagtcaaa cctgacattt
     7321 cataagatgt acctacaacc gtcacagaaa gaatagttat aaagcttaac gacaataatc
     7381 tggcttcttc agatattata taccagctca ctactatgtc tttaaaaaca aacagcaagg
     7441 ctcctgtaaa aagtcctatg ccaatgtata aaagctgtaa agtaactgca tatggtttta
     7501 tcaaatccgt ctttttttct ccaattagtt tggatataag cacagaggag gcattagcgc
     7561 ttccgtaggc aacaactgac agaatctgaa acaatgtaga tgcaatgctg tttgctgtta
     7621 tagaagactg ccccatatgc cctagtattg aagtctgagc agccattgca actccccata
     7681 tcccgttaga cagaaatacc ggaacacctt ttttcaaata tgctttaaaa agtgaaatat
     7741 cagtacttaa aatatctttt aattttaaat taattgtttt atctgataag aatacatata
     7801 aaacaacaaa tatcatttcc aaggctcttg atatcaatgt cgcatatgct gcacctttaa
     7861 ttcccatagg tttgaatccg aattcgccaa aaatcattgc ataatttaaa aatatattcg
     7921 tcactaaggt agaaaaggat acataaaatc cgattttagc tttttccaca cttcttaaag
     7981 ctataagcaa tgtggttgtg attgcgaaca taggatatga ataacatatt atttttaaat
     8041 attcagcacc gctattgacn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc
     8161 caagcattat actatcagcc atgtttacag aaaatacaat cagattctgc aatgccagta
     8221 ctgatgtgag agagaagaat gatttataga aatttttatc tttaactatc aatttgctct
     8281 taaacatata aatattatta tacttcaaaa aaagttaaaa aaatcaattt tgtcacttca
     8341 atgatacaaa attgaaaggt atattatatc taacaaaaat tttaataaat tatatggggg
     8401 agcaaaaatg ataaacttca gctacatagg acaacgcata agaacaaaaa gaaaagaact
     8461 gaaaatgaca cagcaggaac tttctgagat ggcttcaatt tcacctgcat acataagcag
     8521 aattgaagga ggacagcaga atattacact ggatacaata gaaaaaattg caaaatctat
     8581 tgatgaggat ccgatttatc ttatcagtgg cgtaaaagat gatgatgtga attatgttaa
     8641 aaaactattt aacagagctc ctgctcagtt tctctacctg tttgagcttg ctttggtgga
     8701 actagaagaa aaaggtatct taaatattaa gccttgacac aagctctgaa gcaatttcaa
     8761 attctatatc aataccttct tcagtttcag tttttgatat tacttttgca tttgaatgta
     8821 taaatgagag aagtttgcca ttttcaaaat ctactttcag tttcatgtta atgaatttgt
     8881 ttttgctggc tttatccatt aaagataaaa ggtcatctat accctgacca ttcgcacagg
     8941 atatatatgc tctagggcaa gttgaagcat tcaaaatggg gtcttttaca agatccgttt
     9001 tgtttacagc ttctatgaca ggtacagtat ctgcaccgat actttttaag atggaataga
     9061 cttcatccat ctgtctgcta gcatccggat tagatccgtc gattacatga attatgcagt
     9121 cagcatatct aatctcatca agagttgatt taaatgcttc aattagcttt actggaagtt
     9181 tcgctataaa acctactgta tcaataagca taacttggct atttgaagga agtttcaagc
     9241 ttcttattgt agggtccaaa gtagcaaaaa gcatgtcttc agctacaacc gaactttcag
     9301 taagaaggtt tagtaatgtt gatttacctg cgttggtgta tcctgctaaa gcaaaggtaa
     9361 aaaagctttt tcttgtttta ttatctcttg tgaggcttct tcgcttgtcc accttgtcaa
     9421 gctgttcttt aagatatgtc attcttcttc ttatgtgacg tctgtcagtt tcaagtttgc
     9481 tttcacctgg acctcttgta ccaattccac caccaagtct tgataacatt tgaccttttc
     9541 cagtcagtct tggatacata taacttaaca tagcaagttc tacctgtaac ttgccttcat
     9601 tagtaaaagc tctttttgca aatatgtcca atatgacaga tgttctgtcc agaactttca
     9661 cattcaattc cgattctata ttattctgtt gagaatatgt aagttcgtca tcaaatacaa
     9721 cagtatcagc ctgatactga gaacataaat gcaaaagctc acgaagtttg ccttttccaa
     9781 tatagtaagc agagtcgggt tccgatttgt tttgaacaac tgtagctaca cattttccac
     9841 ctgcagtctc tatgagtgat ttcagttctt caatcaatga tttagaataa gtagaattta
     9901 aaccaaccgc tataactctc tctttctgct cttgaatatc aattagttca tcagtttttt
     9961 tataataatt ttccttaagt atcttcatga aatatgaggt ctgctcgata tccatatatg
    10021 gtccaaacag caccggttca tctgattcat caacaactgc acaatacatc atatccatag
    10081 taccgtcgtt tttaatgctt actgcagcca ttgcagataa agcggtattt ttaagggtct
    10141 gaatatcaac atgagataat atagcgcttc catttggatg agtatgtatt agccttatac
    10201 ctttggaagg ctcataatct gatttcacta atgatgagtc acctactgct acttctgtta
    10261 cagtattttt cttatctata tatactgcaa cttctctttt tgtttcatat gtaacatagc
    10321 atatcttagt aattgtatcc aatgaacagt aaaggtctga atcaaactta tcttcatata
    10381 tgcttaaaag catgtcaagc atgctttttt ttaagccttc agtatttcct gatactaatt
    10441 tatccattat tctcccgcta cgtttatatt tgaaatcaat aacgacggag atcctatatt
    10501 accattagtt ccaaaggtaa gatcgtcaga tacagatata acgtttttta acaattcaaa
    10561 atagttgcct gatactgtaa tctgtctgaa gtatttatct ttcttgcctt ctttaacagt
    10621 gtaccctttt gctggtaatg aaaaatcacc actaattgca ttaaaacctg aatgaagagc
    10681 tgaaacttcc gtaataacta taccatcgct cattttctgt tccaattgct caaatgatat
    10741 ctcgcttttt ctaaaataca gattagtagg tgatacacca atattgcttt tatatgagcg
    10801 acttgcattt ccagtacttt ccgtcttatc aatccttgcc gtcttcatat tatgtagata
    10861 tgtcatcaat ttaccattag atataacttc tttgtatctt gttgcaacac cttctccatc
    10921 aaatgatgat gttcctgact gtccaggcat aaaaggatca tctacaatag ttactgattc
    10981 tgatgcaaca tcagttccca gcttgccttt caagaaggaa ataccttttt gcacagcacg
    11041 agctgaaaaa catgaactga ttgctgataa tatatctgat gatactgtat ttagcaatat
    11101 tgccttatat gcaccgcttt ttatcggttt agccccaaga tatgatactg ctttttcaac
    11161 agcttctttt gctattttat gtgcatcgaa ttgtgaaaaa tcattctcaa tgcaaaaagc
    11221 gaacccatca ctcatatcat ctgcttgctg agctagagcc tcaataaagc agtaaataga
    11281 atttcctctg tccttcagat ccaacccttt agtgtttacc agacctgtat tgcctgaacc
    11341 ggaaccaaaa aagcagtaag gacaggattt aatcctgcta tcaagcgatt tagcaatttt
    11401 ctccatttct ttagcaaatt ctatttttgt ctcaacactt atctcatcta attttggaaa
    11461 atagttgtct atttcttcat attccttgga accttcataa attatgtcct gttctttact
    11521 gtcatttatc tttgcattct gcatagcttc tcttaatagt atatcaatag aatcttctgc
    11581 aattttttct gtataggagt aacccatatt cccttcatat actcctctaa aacccatacc
    11641 tttgctgtca gctactttgt aactnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11761 nnnnatttct gatcctttac caatcaatgt agctcccctg actggttctg ctatcttacc
    11821 atttctaatc atatatcctt cttgaactga aaaattgaat tctcctgttc ctggatttac
    11881 tgagcctcca cccatttgtt ttgcataaag tccaaaatca gtcgattgta ttatttcttc
    11941 tttcttactt gaaccgttat caatgaaagt gttactcatt cttgatgtag gagcatatct
    12001 gtagttctgt cttcttcctg aaccggaaga ttccattccc atacgtttcc cattcagttt
    12061 gtcaatcaga taacctttta atatgccatt ttcaatgaga atatttttcc tggtttcgcc
    12121 accctcatca tccatattag ttgtacccca ttgatttggt aatgttgcat catcaatagc
    12181 tgttacacag tctgctgcga ttttctcacc taacttgcct gaaaatacag aatttccttt
    12241 tgcaactgat gtggcttcca aactgtgacc gcatgcttcg tggaatataa ctcctccaaa
    12301 accattatca ataactactg gatatgcacc agctttacaa taacctgcgt aagccatctt
    12361 ttttgctgtt tctgcagcat ttctggaaat atcgacaatg tccatttctt taacaaattc
    12421 aaagccttta tgagcaccag gcccagcaaa tcctgtctgc atctcattat tgtattctgc
    12481 aacagctgat atagccattc ttgtatatac tcttttatca gtcttaaaca atccttcagt
    12541 atttgctaca agaatattct gaatcctgtc caaatatgtt attgatgcct gttttatgca
    12601 ttcatcatat cccataatag tcgaatatgc ctgtttcata tattcaagct tttcactttt
    12661 atctacaaga tcaggcatta tctttatagg atttctgtta aatacatcac tcttacaaag
    12721 atttatagtt atatcttttt taaccccctc aagagccgct gcggctttaa gggctgtatt
    12781 gatcatttct tcctttataa aactgttagt atatgcatac acacaattca aaccccttat
    12841 taccctgatt ccaataccga aatctcttcc tgattgtgtc gattctactt gtttgtctac
    12901 ataattgata ttgctagcaa cagtatcatc taaaaaaacc tctgcaaaat cagcaccttt
    12961 actcattgca gcggtaagta catcaaaaat cgtattctta tcaagcattt ttgtaccttc
    13021 tttcaaaata ataagtaaat tatacacgaa aattaacatt atttcattaa aaatgagatt
    13081 gtgcattata atatttctac caatttatta tttagcttat gttataatct atccatgata
    13141 ttgaaataat catgcaaatt tgctaatata tccaacaaga gagtatatag tgaagaaaat
    13201 atttattgca ctattcacat taattatatt gtcagtactt ccaatacctt catcagccca
    13261 gccagtaggt attattcatg agcctggtga aatttatcaa ggagatactt tatacttcgt
    13321 agatgttaat cttacaagca acacaggaac aaccgataca ttcaaactag ctttgttaaa
    13381 aggagattac ctggcttcac ttcctctttc ccagttaaaa tccgatcttt ctaataactg
    13441 gacaaaactg atacctgaaa tacaaacaca aagaaacaca ttaatctact taaaagaagg
    13501 atcagatatt attaaatcag taacaagtga ggattattca ataagtcaga tagtattaaa
    13561 tgcagccaga caatactatg gcataaataa tgctcagata tcctctacag cttatcttga
    13621 taacaatata gtattatcca ataacgtttc aaatgaagac gggcacatta tgtatgatgc
    13681 tgcatcaaat actatgcaat tacactatac atatgacata agtgagcaaa cgatttttat
    13741 taatgagaat ggtgtatcta atgaatattt gttgaaaaga tataatatgg tcgtttacag
    13801 agtcatatgt atggcagttg acttaactta ctcaataaat caacctactc caacgccgac
    13861 atctactccg acacctacac ctacccttac acctacaccg acaaccactt tgagccctga
    13921 accatcggtt actttatcac ctacttctac tataagtcct tcaccatcac caactgctag
    13981 cagtacccag cctgtgaatt catcacaacc atcaccttca ccagaactat caccaacctc
    14041 ttctccttct ccttctccaa gagtagaagt tataaacaga gaagataaac gatatgatga
    14101 tatctggaca ttcgtgatac ttcaggcgct tataattatt ataattatca ttattgtttt
    14161 atccaggaaa aaaagacact aatcagtcgg attcagtttc cagccatatt ccatatagtt
    14221 catcaagctt actgttaagc tgtttttttt gttctgtaag ttccataagc ttttcataat
    14281 cacttgagtt attattcatt tgtgatatta cttcgtttaa ttcattctct aaagttttaa
    14341 tctgctgttc acaagttcgt tttttaagat cagtctttct cttttctttt ttatcatcaa
    14401 aaggcttttt tacgaatgtt cttgatattt tgtcctgttg aactaatgct tcttttttag
    14461 caagataata atcataatca ccttcatata cgttaaacat accgttttca atttcgaata
    14521 tgcgtttagc aaatttattt atgaagtgcc tgtcatgtga tatgaatatc atactacccg
    14581 aaaacatcat taatgcatct tccaattctt ctcttgcagg aatatccaaa tggttagtag
    14641 gttcgtcaag aatgagcaga tttactccat cctgcatttc taaaagaagc ctgagcctgc
    14701 ttttttcccc tcctgaaaga ctgcctactt tcttgtaaac gtcatcatca gaaaacaaga
    14761 actttcctaa tacgttacga gctgattgta aagtcattga caactgctca cagacaagct
    14821 ccaaaacagt ttcatcttca ttaggaaatg tgacatgctg ctcaagatat gcatatttaa
    14881 cacttttagg tcttttaact attccgctat cagcttcgat ctgacctgta actattttaa
    14941 tcaaagtgct tttcccagcg ccgttaggcc caattaatgc aactttgtct ccacttcgca
    15001 taatgaaaga aacatcactg aacagaactc tgttttcgaa ggacttctca atgcttttta
    15061 caaaaaatat ctctttagca actttttcct cttctaaaaa agccaatcgt aattcttttt
    15121 ctgtaacagg cttatcgtaa atgtcaagtc tctccatcct tctttcaagg ttctttgctc
    15181 ttttgattgc aatctcagta ccataatttc gcatcttcaa agctgtttgc cttaatctgt
    15241 taatttctct ttcctgtctt tcatatagtt ttagatctct ttcttcagct tcgattttct
    15301 gcacttgata agcagaataa ttacccttat atgattcaac tttaccatca tacaattcaa
    15361 ttactttcat agcagtgtta tctatgaaat acctgtcatg tgatatatac aacacagcac
    15421 ctttgtattc tataatatat ttttcaagcc attcacatgc attagcatca agatgattag
    15481 taggctcatc caacagaagt atgtcaggat tcattaaaag aacctttgca agcataatca
    15541 tagtaagctc cccaccgctt aacttagcag cctgattctc tataaccgca tctggtattc
    15601 caagtccttt agttacctta ttaaatttct cattcatact atagccttca taaaattcga
    15661 atctatcatt taagatgcta taatttctta tgtgttcttc attatcaggc tgtattgtca
    15721 taagctttct caactcagac atctgatgtt ctacttgttt tatatgctca aatgcttctt
    15781 caattactgt ttttgatgtt ttagaaccaa aatcggaata aatctgtcgc aaatatccta
    15841 tccttaaacc tttttcgaca tatactgagc cgctatcata atccatatta tctgcaataa
    15901 tattgaatat ggtagttttg cctgaaccgt ttttaccgat taaagctact ttttcattag
    15961 cgtggatttc aaaactgata tcctttaata catcaaatcc gccataactt ttattaacat
    16021 tgtttaaagt aataattgcc atttaaacct catagataat tatacataaa aaaaccagct
    16081 tgttaagaaa atacataaaa aagccggatt atatgtaata aatgttatta agaattcttc
    16141 attatagtgg tttcaactat atttgctaca tgttcgcagg aatcacagca tttttccaga
    16201 ttctcgaata ttgacttcca gacaagtttt tcaacagcat ctgcatcgga agcaaataat
    16261 tcattcatag attctacaaa aagcttatct ccttcttctt ccaaatcatt tacttcgatt
    16321 attaaaccct ttatagtttt gcttctggag aaattacgaa attcaataaa agtttttctt
    16381 aaagcttcac agcatctttt tattatatct gtaaaatcca gtgctccttt ttttattgtc
    16441 tttatgttat acatgtatat ctttattaaa acatcttcaa tattgtcagt aacatcatct
    16501 atttcctgag ataaatttat tatatcctct ctgtctatag gagttatgaa ttcttttatg
    16561 agtttattta caagagtatg cttctgcata tctgcctcat gttcgacatc atgcatgctt
    16621 ttaagcattg tttgtgcttt tttaagatca tagttatata ctatttcaca taacaggtct
    16681 gctgccctgc agttgtattc aaccagttct acaaatttct gataatagtc aaaatctttt
    16741 ttgttaaaca tcatattctc cttaaaatac tgctaaaaac aatttagcca taagataccc
    16801 aataagcccg caacctggaa aagtgaatat ccaggtaagc accatttctt ttaccactga
    16861 ccactttacg ttccttattc tttttgccgc tcctacaccc ataattgctg tagtcttggt
    16921 atgagtagta ctcacaggta ttcctgtaag agaagaaatc agaagacatc ctgctgcggc
    16981 aagatcggct gaaaatccct gatatttttc taagttaacc atattcatgc ctacagtttt
    17041 tataatccta tatccgccaa tagatgttcc tattcccata acagcggaac ataaaatcat
    17101 catccacatg ggaaatataa ggttagtggc tgaactcata ccctgtgaaa gataaatgcc
    17161 taatataaag atacccataa atttctgtcc gtcctgagca ccatgcataa atgccatcaa
    17221 agcagcgcca ccaatttgtg ctccttgaaa gagtcttcct gtttttgttt tatttactct
    17281 tttaaaaata aactcaactg ctcttacagt tagaaaaccc attaaaaatc ctaaaaatgt
    17341 tgaaaaaagc aagccatata aaaccttgac tatttccgga ccatttattg atgaaaaact
    17401 gttacttact gctattcctg ctcctgcgat tcctgcgact aaagcgtgac tttcgcttgt
    17461 tggtatacca aaaatccatg cggtaaccgc ccaaataaca atagcgcata aacctgcaca
    17521 aagtgcaata agcgcagttt tgtaatcatc accgaaatca accatattaa aaagtgtttc
    17581 tgctactgtg gaattaatca gtgtcataac caaaacgcct agaaaattaa aaacagcagc
    17641 catcaagact gctatctttg gagatatcga tcgagttgat actgctgttg ctattgcatt
    17701 aggcgcatcg gtccacccat ttaccagtat aaccgccata gttagcaaag ttgtcacaag
    17761 cagtaatgga ttactgccaa gactttgtaa atatgtgccg aattctattt gcataaatta
    17821 attccttgta tacattttaa catcatcact tacttcaatc aatctgtttt aagttgtttc
    17881 tttaaaagat atttatctat cttcttaaca agctcctcag gaatctctct tgctactgga
    17941 aacatatatg cattaactat tctgtcagaa aactgagcaa caaaacttgc tgtatattca
    18001 aggctcttag cagagattgg gtcaattaca tcatttcttg tgtgacagga aactgctcct
    18061 ggagctccat atcttacaaa gttaactgct ggaatccctt tatctgcaaa aggtatgctg
    18121 tcagatgagt atatatcatg ccttaaactc atcggatggc cgatttcctt atacatgtac
    18181 tctatcatgt ggcataattt ctcttctgca aggacaattg ctgtatccct gccaataaca
    18241 ggacctgcaa gatcaacatt tatacataat tttatattat caagcttatc agcattttct
    18301 tttacataat ttttgcttcc gaaaagacct ctctcttctg aaccgcacca aatgaatctt
    18361 acagttctta aaggaggatt ttttacaaag tatcttaaaa gttctaaaag tattgcagaa
    18421 cctgctgcat tatcgtacat tcctttactg aaaaccactg aatcataatg tgctgtatag
    18481 actataacct catcaggata ttttgtacct tttacttctg tgataatatt ctttgaatcg
    18541 ccttcatctt ctttctgctc taaaacaagc tgaatctttt ttggttctga tttcataaga
    18601 tttacagcat ctttcattct tatgcaaaca cctggtatct gaccattttg aatatgtcct
    18661 ttccttagca ttctttcatc aagatcagtt ttcttagggt catcaaaata atcgcctgaa
    18721 ggtgttataa atccagctac accagctttt accagtcttt catatgtttc ataacctaaa
    18781 gaagatacca atacaatttt tcctttggca tcaataagat caatttcatc agcagtttca
    18841 acatatttga agtcagctat aataccgtct tttgcagaat ttccgctata tccgtatcct
    18901 ttaatattaa aagacatacg cttaggttct aaaacttcaa gacttgcttt aactatttca
    18961 taacaaggaa ctttgaaata ttcgtaaact ggttccaaac ctgtttgctt tatctcctca
    19021 gagataatat cagcaccttt tttctctcca tcagtagtgg aaagccttat gaaattcatt
    19081 tttttaagtg tttcgtaaat tctttttcct ctgatatcca ttcagtttta ccatatttcc
    19141 tttcactatg ctttattata gtattcattt accgcatcaa aaaatgcatt aatattttcc
    19201 catggagcca ttggtggtac gtcacagcca gtagaaataa tgaaattatc atatttacag
    19261 cattttttca ttatcttgag agtttcctct cttattgagt ctacagtacc atttttaatc
    19321 tggcctgcag gatctacatt tcccatagct attatatcat ttggtatttt tttcatcata
    19381 tcttccatnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    19441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnta tatgctttta
    19501 tatattcagt tataaaagaa gtaactttct taagcacttc atgaactatt tcaggttcat
    19561 cataacaaag caccattgct tctgttacat ccaaaagtct tcctgcaaga gaaaacggcc
    19621 caataacgcc agctaacact ggtctgtctg taatcagttg ttttgctttt cttattgctt
    19681 caatatatat ctttgttctt ttacacccca cctcaggaac aacaagattt acagcatctt
    19741 ccatagttgt gacaacatga cctgtaacag tcggcacttc atcatctgat gcaataatat
    19801 ttgctccaaa acattctgct tcaactgaaa ggtccattaa actcacagat gcagcactat
    19861 ctgttttatc cgctacagct ttcattccct gtgcttgaag ctcgtaatct gatataagct
    19921 gttttacagt tatatctaaa agctgaattg aaggaaatga taacacaggc attgcttttt
    19981 ttactttact gtttttcata tcctccagcc atttgaacat atccaaattt tatacctcca
    20041 atattatatt aataacccat atttgctaaa ctgtcttcaa ctaaataaac cagattattc
    20101 tcagctcccc agtcaagccg caaaaataca cctgtacctc cgcctgaacc tatagctgaa
    20161 acataaatct catcattatc tttattcgaa atcataacaa cagtaagact ggctctgctg
    20221 ctgtttctca taaaatattt atcatatacc ctgaccagca tttcacatcc atttacttct
    20281 tttcttgctt catctacaag attaattgaa acagcgctgt tggatatatc attatgtaac
    20341 ttttctgaaa tatcatagaa actgccctta acaacttttt caaatttaga catttttccc
    20401 tccaaaatca ttccatgaat cattttaaca taaaaacagg cacatgcctt aatatattcg
    20461 cacatacctg ttttttttgg agcgggtgac gggaatcgga cccgcacagc cagcttggga
    20521 agctggaact ctgccattga gctacacccg cttatatgaa gattctacat ttttttatta
    20581 atttgtcaat gatgaagctc tatgttaatg ccttattttt ttcatcttcc ttatataatt
    20641 tattccattt tataagtcct acaatgttta tagcaagata tggaatattt tgaagaacta
    20701 atgtaatctg agtggtatct gcacttaatg tgcttatcca tattgctgaa aaaatcaaac
    20761 tatatgcaat ccagaaatac cactgttcct tgattcttct tgaagttaaa aatccagcag
    20821 caactgatgt aaatgtaccc aaagcattaa tatatggaag gttggatttc aataaactta
    20881 atatataccc atacccgaca gttacaacaa cacaagcaat cgctaaaatc acaataagtt
    20941 tttttgttat tctgaatatt tgaaaatctg tcttgacgct tttctttcct aaccagcgaa
    21001 aagatgaata gatgtataac ggaagcagat aaaaagctgc aagtactgct tctccaataa
    21061 atctgtttcg ccatgacaca aatgcataaa gggttatctg aactatatag aagaggtaac
    21121 agtatttatc cgctttcata ttcattatcc cggcagtaat tcctacgatt gaaattacta
    21181 aagaaattaa cgcagcgtca aatattattc ctgctgtaat tatcagcacc attgatgcgc
    21241 cataaaatat gatatctggt ttcgtccagt ttctaaacat tcactccatc cttctaacct
    21301 attatatatc ctattaatga ctttatgtta aggaaaatta tcaatgctcc cactaatgcc
    21361 attaaaggtt tcatgggaag ccttttaact aaaaaagctc caaaaggtgc ggcaataact
    21421 cctccaatta taaggccgat aattgcatac atactttgct caaggcctaa aaaagatata
    21481 aacataatag attctgccaa agtaacaaaa aattctactg catttactgt acctatagta
    21541 tatcttggtt cgactcctga agctattagt gttgatgtta caacaggacc ccatccacct
    21601 ccaccgattg catccaaaag accgccactt aatcccaaag gataaatcca cccgcttaag
    21661 tttttaggtt tttgttttct gaatattttc acaataatta taatacccat caccacaagg
    21721 tatccattaa tatatggttt tactgtattt ccgtcaaaac ttgttaaaag atatgcacca
    21781 acagctcctc cgattattcc tgggattaga agtttaaaaa atattccttt gtttatattt
    21841 ttaagcttcc agtgtgatat acctgaaaca agggtggtga atacttcagc catatgaaca
    21901 cttgcagaag caagcgcagg tggcacgcca aatgtcagaa gaaacgagtt acaggacact
    21961 ccatacgcca ttcccaacgt tccgtctatc gtttgcgcaa agaatcctac taatatgaat
    22021 atccatatat ttgttggtat tgaagataca gcctggccaa agcttgccaa tacttccatg
    22081 taattaccga acagctacct gctctacctt ctttcttatt atctctatag catcttcatc
    22141 attaggcaag accatatcac attctatatc agtggtacat tcatcaccca gccagaaaac
    22201 caaagtgttg tcattcttag ttacaagttt tattgtttca acatcatatt tagttagatc
    22261 acttgcagtt acaataagaa taagcccggt atcaagaagt atattagccg cttctgctaa
    22321 tcttcttata ttctcgccag atctgttttc tttttcccct ggaggattta tatctgcatt
    22381 caatccgtat ataacacttc ctataccaag ataatagaca ttcttaccct catcaaacaa
    22441 acttctttcc aaagcttttg caaaaagttt tttgtttgta tctttggttc cagtaagaat
    22501 tatcaaagct gcattttgtg agaatctttc atttctttcc ggcaatgtaa cataaccatg
    22561 ttcccatttc ccttctctta acattacttt agctttttca tatctgtgaa gattgtcaac
    22621 agtctcagtg aatatacctc cacctgatat ctcatattca tcaacaatta cgaaccgacc
    22681 tgttgatgat aacaaagctg aattatcaaa agcacataat tttgaagtgg aaataatgca
    22741 atcagctaca tcatgtttat ctatttggtc cttttcagtt ttaataagtg tggaagcatc
    22801 tattacgctc aatatgttct caagatagca ttctgctttc attgttccag tctttaaaaa
    22861 atatcttttg tctttcacca taggtttctt accaagccag aatagactta ccttcatatg
    22921 ttttcccata agaggcggag tctggtctga tttaaagcat agctcccctc ttttaacata
    22981 aatctgttca gtcatagtaa aacctgttgc atatcctgcg cttgcttttg tggctgtatc
    23041 atattcactc ataggttcta ttttattgac atgagttttt ttccccgatg ggtagaaaac
    23101 tacttcgtca ccattattta tagtacctga ttctaccgta cctgcaataa ttcttctatc
    23161 atccgcatca ttagtaaatt tgtatacatc ctgtaccggc attctaaaag gaagtgaatc
    23221 tagcggttta gaagctgtaa acttatcaag catggtaagt acagtatcct cttcatacca
    23281 tggcatatta atgcttcttt ttgcaatgtt atcaccttcc ataccgctta caggtatgaa
    23341 gccttgagac tgaatcccaa ttttatttaa aaactcgtta tactcattta caattctgtc
    23401 ataaacctct tttttgtatt tgacattatc cattttgttt acaagaacac atacctgctt
    23461 tattccaagc atagaaagca tatatccatg tcttcttgag ttttccttaa ctccttcagc
    23521 agcatctatg actaaaagag cagcatctgc tcttgaagct cctgttatca tattctttag
    23581 aaattctata tgccctggcg catcgattat aatgtatttt ctcttttcag tcttaaaaaa
    23641 acttctggcc gtatctatag taataccttg tgatcgttca tctttcagtg catcaagaag
    23701 aaaagcatac tcgaaaggtt ttgcattatt tctgcatagc tgctttatct gttcaagctt
    23761 tccttcaggt aaagaattag tatcagcaag cagtcttcca ataatggtgc tttttccgtg
    23821 atcgacatgt cctactatga caatattcat gtgctcatca ttctttttcg tcatatcaca
    23881 tatacccgtt ctttcttaac tcttccagtc cgccgccatc ttctttgtcc tgatctcttc
    23941 ctgaccgttc tgctatattt gcaaatttac ctgttcttaa ctcttctata atatcatcta
    24001 aattcttagc ttcagagtct actggttttg tacatggata gcaacccaaa gaacggtatc
    24061 tcttcttttc accctgatca aaatacaaag acacaacagg cacattttct ctttttatat
    24121 attcccaaat atctaattct gtccagtcaa gcagaggatg aatacgtaaa tgggtaccag
    24181 gagcaaaatc cgttttatac tgtccccaga attccggagg ctgatctgaa atgtcccagt
    24241 cattgtgagt atcacgaggc gagaaatatc tttcctttga acggcttccc tcttcatcag
    24301 cacgcactcc cacaattaca ccggtatatg gttcagtatt tttgtcaata acatattttt
    24361 tcaagttatg atccattctg tatcttggcc agtcacctgc taaagtatga gcaagtgctt
    24421 ctgttttaag atttctgcag cactcaagcc gtgtacagtt gccatctgga aatgttcttt
    24481 tttcctccaa agcttttctg ttctcaccgt atatcatgtc cagattccat tctattgcca
    24541 gtctgtcacg atactcaatc atctgaggta ttttataatg agtatctata tgcactaaag
    24601 gaaaaggaac atgtccgtaa aatgcctttc tggcaagaca caataatact gtactgtcct
    24661 ttcctataga ccataacata cataagtttt taaaactgtt atatgcttcc cttataatat
    24721 gtatactcct tgcttctaat ctatctaaat gatccatatt aaataactcc taataaatat
    24781 tccataagtt tatctgcaca ttcatctata ctgaattttt ctgtatcaag aagataatca
    24841 tactctatag gttcttcaaa tggagagtca atccctgtgt atcctaatat ttctccattt
    24901 aaagcctttt tgtacaatcc tttgggatct cttttaacac atgtatcaat atctgctttt
    24961 acataaacaa gtataaattt tttactttta attcttgcaa gatttctcat tttttcaaaa
    25021 ggcgtgattg cacttactaa aacagttacc ccgctgtctt caaacagagc tgcaacttca
    25081 gctatcctgc gaatgttctc cgatctgtcc tcttctgaaa accccaaatc agagcataaa
    25141 ccatgcctta tattgtctcc gtcaagaaga taagccatta ttcctttatc aattaatttt
    25201 ttttctatta ttcgtccgat tgtagattta ccgcttcctg aaagaccagt tagccatatg
    25261 acagatcctt tttgattagt ctttaactgt ctgtattttt tatccagttg ctgatgccat
    25321 acgatatttt ccataacgta cacctccaca tatgccatat gtgattgtat atgggtaata
    25381 tagctttgtc aaacaatata aaacaatatt ttttggtgtt gcattgatta atcgattgat
    25441 gtaaagtact gtttgtgtaa ggaggattag tatgctaata acagtaattg gaagaggtca
    25501 ttcaggaaca agagctatat cacagacact taaagacagt ggtgttttta tgggggaacc
    25561 tttaaataag tcatatgatc ttgttccagc cggggatatg tatgaagcat gccgtgtttt
    25621 tgctaaatac gttacgtatc ttggtgatat gaaatgggac ttttcaaaag taatgtcaat
    25681 ggatccggat cctgcattcg taaaacttat cgaaagctat ttgtcgtcag tcttatcttc
    25741 aaaaagccaa aacaagggtt ggaaaatacc tgaaacaact ttagtatatc cttggataat
    25801 gaaaatgttt cctgaagctt tttatattca ctgggtacgc gatcctcgtg actgcattat
    25861 tggcggtcat atgaccgatg atttaaatga tttcggcatt tcatatgata aaagtgaaga
    25921 tataaggaaa aacagagcta taagctggtt ttatcaaaga gagattgtac gacattcacc
    25981 agttccagaa aaaattgcaa gtgtcagatt tgaagatatg gtatttcgtc aggatgaaac
    26041 tttgcaaaaa ttggaaaagt ttttaaatat cccattggta aaaataccca tgcgtaccga
    26101 ttctgtcggc agatatctta ctgacgatca aattaacatg tttgatttct tcaaaaatga
    26161 tatgacagag tgcggttacg aattctgata catatcatct ttaaatatct ggaacaatat
    26221 accacccgta gctgaagcag gctgcgggta ttttaataag taacataaag taggagctat
    26281 atcttctaga tttgcaggtc tgtcgactaa ctcacctttt tttatattct ttccggaaaa
    26341 catacataaa cagcgaatat cgccagtttt agtttttgct gatggtatct ggcatgcatg
    26401 tactcctcct atatatcctc ctgtcgaatt acctgaaaga ccatatatga catctccaac
    26461 cctttcagtg cctaagccta caaaacctgc ctgttttttt tcaacagcaa aagcaagata
    26521 gcattcatct ttctcattcc agtggtattt atgcagaatc tttataattt catttacagt
    26581 cttgtcataa tcttccgggt caactatacc ttcaggttct cttcctttta gattaatgta
    26641 tatattaccg cagcccacgg gaaaagcttt tgtatttttc cagtcaattt ttctgtcacg
    26701 ccaataaacg tgttcattct taggatgtgg aatatatttt aaaaatcctt ctttctcaaa
    26761 aaaatcatgc acaagcactg tcctgttaaa ccctactgca ccatggtctc ctatgataat
    26821 aaaaacggta tcttctccaa ttacattttc atacagccag ccaataaaat catccagcat
    26881 atcatatgct ttttcatata cataacgggc aatttcaacc tgttgttcgt tacttttttc
    26941 cacgccctcg tatagtcctc taaaaacatg atttactgta tcagtatagc cttgtacaaa
    27001 tgccataatt tcaactggat cattttccat tgaatctagt attatctttt taaaccattc
    27061 gttcttaaat gcaatcagtt cgaatgcagt atcaatataa ccatctcttg taagaaaatg
    27121 cccatagctg tttgatgagc aaatttcatc aatttcgtta actctattgg caaattcata
    27181 tggtgataat gcattaaaaa gattgacaga ggaggtaata aatacaaaga accctccttt
    27241 atcatttgcc ttcattgctt ttatgcgaaa tctgtaggaa ccctcgccta aatcatcttt
    27301 caaagttctt tttataactt ctgaccagtt acccataggt atctcataat actggccatt
    27361 ttccatatca gcttcattaa aagcaatctt aataccttca tcataaatac tcattatcca
    27421 gtaaaaaggc tcaatattat ttggattttc caaatcattc tttttttcaa cataaagttt
    27481 aaaatatccc ttttcgctct tttcatacct gctttgagtt aatttaattt gtggctgcca
    27541 ctttcctgaa ggtgccatag tctgttctgg tatttttgga aaaacagata atacatcagt
    27601 atcaaaaaga tataactgtt ccgatatttc tttttgcatt cgcatagtct gctctgtttt
    27661 tattgctttc tggtcagcca cgcttccaat tcctcctatt tgatgcacaa ggtcggattt
    27721 tgctggtccg gatacacaga aatctgcaat taaagacttt ttacctttct tagctgctgc
    27781 ttcccagaac ctttcggcca taagataatc cccatgatat gcagttacaa attcattggg
    27841 atatgcacct tcgagcaata tattttcatc agttatctga tgcatatgag gacttgctcc
    27901 agtttgtata gtagcccagc atgtggctga tatagacgga taaacaggac ggcaatcact
    27961 tagcatacat ccgttatcta tcattttctt aaaattaggc agttttcctt tttctatctg
    28021 ctctaataca tacgatgtta ctcctccatc tactccaagc ataaataatt caggtttctt
    28081 taacatatat tctgtcctcc agatttgtta ttacctttta attataccct atcaaaacct
    28141 ttatatttga acaacatata attattcaca caaaatttgt gcatcttgac tgaatatcac
    28201 catttgatgt tgatttttaa taaggctaag atataattct attattaaat actttctatt
    28261 cgaaaggaaa atcatatgat caaattagga gtgaataccg tactattcaa acactacaca
    28321 ttcgcacaag ctgctgaagc tattgcaaaa tgcggttatg atggagtaga aatctctgcc
    28381 atacctggaa tgtgtgagca tcttcaacca gaaaactgga gatcacagaa aaaagaaata
    28441 aaagctgtac ttgaagctac aggattggaa attttggctt ccgaagtcgc atcaagagac
    28501 aaaaaaagag caaaaaaagc atttgaagct gcagaatatt ttaatattcc agttataaat
    28561 gttggacctg gaggcaaatc tggagttgaa aaagattttt tagaatctgt taaaagtctt
    28621 tctgagttat caaaaatggc agaaaaatac ggagtgacat tgtgctgcaa agctcatgtc
    28681 ggagcatcaa tacacgatac agctaccaca ttaagagcaa tggatatgat tgaaagtccg
    28741 aatttcggag ttgatatgga cccttctcat atttacagag caaatgagct accagaagaa
    28801 gccttggcta aagtccttac aagaatgaag cacatacata tacgtgactg taaaggaaga
    28861 ggtccaagtc ccggtctgcc agccctgcag gcttgcggaa aaggagatgt taatcttttc
    28921 ggatatattt ctgaaatggt aaaagcaggc tacaacggtc cagtggactt agaagtaatc
    28981 ggacctgaac aggatatggt tgaagcaaca agaattgctg cagaaagtta cggatacatg
    29041 aatgcctgct tgaaagtttt aaaagcaaga taaccattgt ataaatttgc aaaaggcaaa
    29101 tagaataatt taagtgaaac tataaggaga atataaaatg gcaaagaaac caaacctttt
    29161 gtttttcgga attgacagcc taagaagaga ccatatgagc ctatatggat atgacaggct
    29221 gaccacaccg aatattgaca agtaccttaa agatggcact gttttcaata actgcttttc
    29281 accgcatatt ccaactacac cagggtatgc aaacatgatg acaggatttg actgtttcgg
    29341 aacagatata gtagctcttc gtcataaagg tgagatagca cccaacgtta aaacattagc
    29401 agaaatttta tcagaaaacg gatacaatac tacaaatgta ggatttaaaa atgtatcggg
    29461 cagaggtttt gacaactaca tcgaatttga aggctggaaa gccgaagaag gagatggtag
    29521 ggctcataaa gcagaaaacc taaataatgt agcaattcct gaactggaaa gattatcaaa
    29581 agaagacaag ccattttttc tattcctaag acatatggat cctcattctc cttatcttcc
    29641 acctgctcca tttgataaga tgttttacca ggcagatgct tttgatcctg ataaccatac
    29701 tcttgaccca gtttattcct ttaaaccatt ctgtgattat ttcgtaacat ggttcccaaa
    29761 aggctgtact gataaggatt atataatcgc acagtatgac tcagcgattg catatatgga
    29821 ttcatgtatt caggcaattt tacaaaaagt aaaggatctt ggattggaaa atgatacatt
    29881 ggtagtattc acttccgacc atggagagac tctatatgat catgactgct attttgacca
    29941 tcacagtcta tatgatccta atcttatagt tcctcttgct ctttcatttc ctggtcatgt
    30001 gcctcaggga aaaagagttg atgactcatg caggcttcag gatgtaactc ctacagtttt
    30061 agacatactt ggaattgatg caaaaatcga ttttgatggg agaagcctta tgcctttggc
    30121 aaatggcatt ccacttccaa aacaaaatga attctatata actgagtgca cttggatgag
    30181 aaaacacgga tggagaacac ctaagtggaa atatataaaa gcattagaac ctgattttca
    30241 ttacaaaccg gaagtagaac tatatgattt ggaaaatgat ccccttgaat atactaatat
    30301 tgctgaactt agaaaagata tagtcatgat gctggacaag aaaatgaacg acttcattaa
    30361 aacccgcgaa aagcaggtta aaagagaaaa tccaatgtat acaaatctca actggcatgg
    30421 gctaaatctt ggaagaggct ttgaaagttc cgatgaagcg tacaattcca tgcatatagg
    30481 atcaccagga gcagctgcaa agctgcaaca gaaataaagg aggcataaaa atgctaaaag
    30541 cagctgtaat aggcatgggc ccaataggca acaatcatgc tacagcatat aagaattgta
    30601 aattagcaga attagtatca gtatgtgata taagaaaaga cagagctgat gccgctaaag
    30661 aaaaatacaa ggttgctgca tattatgatg ctaaggaaat gcttgataag gaaaaacctg
    30721 atatagtcag tgttgctaca ggaggatttg aatatagttc tgatcacttt atcccaacca
    30781 tgcaggcttt aaatgcagga tgtcatgttc tgacagaaaa acctatatca aacaacttgc
    30841 aacacggtca gcagatggtt gatctggcaa aagaattaaa tcgctgcttt gcggtagatt
    30901 taaatcaccg tttcactcca gctgcacgtg cagcaaaaaa atggcagact gacggattga
    30961 ttggtgatct tctatttctt aacatggcat tatggatagg aagattccag cctcagtttg
    31021 acactgaata ctatcatcta aaagctctta atcctcattc atgcgacata ttaagatatt
    31081 tcggcggtga tgtggaacag gttcattgtt atgcaatgaa ggcaccagga agacagatat
    31141 ggtctactgc ttctattaat atgaaattta aaaatggtgc tgtaggtcat cttacaagtt
    31201 catatgatat tgaaagaggc catccaatgg aaaggtgcga ggttgcagga actaagggaa
    31261 gactgatatt cgaagacatg tggagagaag ctacacttta ccctgccgga aattatgaaa
    31321 aaagggttta cactaatcct gtttttggag ggtttcgaga cttttacgat acttttgaag
    31381 acagaataca tacttttgta agagaaatat cagaaaatgt aaaacctgaa aatatagatg
    31441 gctcgggaaa agatggtctg gaatcaagcc gtattatcca tgcggcaata caatcattaa
    31501 aaacaaacaa accggtaatg gttcaaagcg ttacagaata ggagattaga aatggcaata
    31561 aaaatcggaa tagttggaat gggaggaatc ggtaatactc acgcaaagtg ctataaagca
    31621 gattctctag ctcaattggt ggctgtgtgt gatgtagtaa aagaaaaagc cgataaagct
    31681 gctagtgaat ataatgtaga agctttctat tcactaaagg atatgataaa agctcaccct
    31741 gagctagacc ttgtnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    31801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnncctgtt
    31861 tatattcttc ataaagtagg ctttaacgga agcgatatgt catacaaagc tccagctaat
    31921 ggaggtccta tgaaaagatt tgaaggtcct tattcacatg catatgcatt cttatcacat
    31981 cctttctccc taatgagaag ttttggtggg gatataactc atgtacaggc tttctttgat
    32041 aaacctggag tacggcacag cgcaaatgat cttatgtggt cgataaatgg tatcaatgta
    32101 aaataccaga atggttcaat aggatatctt ttatcacaaa gaggagacgc tatattcggt
    32161 cttggaggtt ggtggagttt cgagatggca ggaacaaagg gcaccttctg tatagaaaac
    32221 tgtgttgaaa agttatatta ctgggatgga gcgaaacctt catcaatggg aacaatggaa
    32281 ccggaagtat tcgatttcgg aactaaagat ttcggcatta cttttaataa caggcttcat
    32341 gctttcttag aggatatgac aaataagata cctccagaac acataagaag ttctggaaga
    32401 gatgcattgg caacactaga gtatacggta gccgctattc agtcttttga acaaggtggt
    32461 gaattaatcc gtccgcgtgc tcttcctcct ttacatggaa cacctggttt tgtagtgtaa
    32521 tgaaaaaacc caacatactg ctgtttttag cagatcagca cagatatgat tgtgtaggat
    32581 acaaaaatag tgatatccag acacctaaca tagacagact ggcaagacaa ggcgttgttt
    32641 ttaacaacgc tttttgtccg acgcctgttt gcgctccagc aaggcagtct tttatgtcag
    32701 ctatgagagc tgagtctatt ggagctttat ggaatttcag ttttataaat acaacaacta
    32761 tccagccaga tgactggaat atagtcagaa atcttaaaga tgcgggatac aagaatctat
    32821 tcatcggtaa gtgggatgta tctcacacat cagaatatga ttatggtttt gatactgtgc
    32881 actcatcaag cgaatacaac gctttcatta aacaaaatta tccaaatgtc gaatatacga
    32941 ataaatggtt tggtgaaaaa aatccaatac catataaaca tagcaggcct aaatattatg
    33001 ctgataaagc atgcctggaa ctgaataaaa tgaaaaacag cgatactccg tggtttatgt
    33061 ggattgatgg agtagaccct caccttccat gcagaccatc tgatcagttt tacgacatgt
    33121 ataaaccctc
//
LOCUS       DAOSXM010000016        32292 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2111
            NODE_880_length_32292_cov_12.532277, whole genome shotgun sequence.
ACCESSION   DAOSXM010000016 DAOSXM010000000
VERSION     DAOSXM010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864178
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 32292)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32292)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:24:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,261
            CDSs (total)                      :: 2,229
            Genes (coding)                    :: 2,218
            CDSs (with protein)               :: 2,218
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 3 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32292
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_880_length_32292_cov_12.532277"
                     /isolate="AMR_MDS_2111"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(39..1931)
                     /locus_tag="PLQ68_03810"
     CDS             complement(39..1931)
                     /locus_tag="PLQ68_03810"
                     /inference="COORDINATES: protein motif:HMM:NF020730.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK71810.1"
                     /translation="MKARRILISILVIAMLTTVIVIPTAITDATRACAAIGIIRGEGG
                     TITDEYLSRNATRAQGMLINLRLRGLESQAKRFNSTENLNDISPLNAAFWKPILAYTY
                     AHPELGFIGDGNGNYRPSDMISGTEIVKIMLDLLGYKQGVDFEWADVKAFAESKGLTV
                     KEGYITIEDIAVTLQETLKLNNKQGKLFIDTLYEQGIINDQRDVKASSETDPSIPYAA
                     RMWIFAYPGGYLYVNATDQYGQEILPPSGTFTSSDTSQIVLGDPRPGVNPLRYVCCDA
                     YGITSGTNTAVISYKSDDGLWNISTPITLDGKSERNTFSSISYNLECPGLLVGKTLKI
                     RTHPKDSQGNTFGLGFNYFYIQVRRTDDNNPYGTEISGDSPVFEITRDETNGMYINVT
                     AKRAGTETLRIIACRGGSYGSTNPNYIEAVYEFTLTAVDDSEVVGCRLNFEYGRHNMY
                     AGAISEEHNLKISISGVTASGLNVLLQSTNDVSLPDAFCYESDGNNIEILEEKGEITV
                     IKPIILDTTANIKVIGKNDNVINVTRVRLGGEAPSLNSIYMSAYYDYVCVYAYDQYGK
                     YIQVPNGTVKSSDTNKIVISGIRTGDEPLKLYYNFTAVGNSPNTATISYVSDDGLYKC
                     ETTVTK"
     gene            2307..3752
                     /locus_tag="PLQ68_03815"
     CDS             2307..3752
                     /locus_tag="PLQ68_03815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK71811.1"
                     /translation="MIFNLSRPKSMLPNSYFWTWDQSTNWVLDDPGIVNFGSWNKYLK
                     NPETYIEDYKRLTDMAQSLGIKGIIIWGFLRDSHGSIEYAKKVASYAKSKGVSIMPGV
                     GTTHYGGVYYEGNNKYNINTFLHANPECGAVVSKEPGAKRAHHAVCPSNPLFKEWIDE
                     GLHWLFKEFEIGGCNFENGDFFSCYCDKCQSQKETWPKNDPNFFRYQSMCYEPALTAV
                     EKFAKENDDLLITWATYCGFLPGNINNIQDKPDSYIKNMGTDRPEIFNRMSYEGIAQW
                     TLTRMVREKEISLMDFLDDGTPSLVFDNPSWLKGITPPSKRSTGFVHQGSQWDYSPWE
                     SKSTRYSLILGRIKEACLRAYESGLEGVSIHGELTSRHIPAALNYLAFSHFTHWPYDT
                     LRAFGQKTLGEVFNNPKEGEDYVEILALWEAGKVDEQLKKRIKERYDSAYSIIYPASE
                     DASESKLTRFFFWNWLYRLVSSFHGIEESFF"
     gene            complement(3829..4695)
                     /locus_tag="PLQ68_03820"
     CDS             complement(3829..4695)
                     /locus_tag="PLQ68_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004626592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HXK71812.1"
                     /translation="MNTDETNRIISLKQVCYTYAKNTPFSKKALDDINLDFYSNEYTA
                     IIGATGSGKSTLIQHMNGLLKPDIGEVLVDGIDTKDKKVQSDLRRKVGLVFQYAQYQL
                     FEETVYKDIEFGLIKRNVPEDQRKQRVLDTAKMVGLTQENLNASPYDLSGGQKKRAAI
                     AGILVLKPEILVLDEPAAGLDPEGTRELFSIIQNLHDNKECTVILVAHSMELVAQYAK
                     RIIVLHDGKVVMDGTRDEVFGNVEQLTSLGLDVPEVTKILKKIKISHPEINDKLYTVS
                     DAKEEIIKHLKK"
     gene            complement(4697..5527)
                     /locus_tag="PLQ68_03825"
     CDS             complement(4697..5527)
                     /locus_tag="PLQ68_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014312137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HXK71813.1"
                     /translation="MIEVKDIYYAYKTQPETTQAVSGISLSVSKGMYIAILGRNGSGK
                     STLARMLNALILPDSGDIFVLGMNSKEKKNELSIRKKIGMIFQDPDNQIVGTRVLEDV
                     AFGPENLGYEREKIIKSVDNALNQIGIYDLKERAVHTLSGGQKQRVAIAGVLAMKPSC
                     IILDESTSMLDPKGQAELLAVTEKLNKEEGISIINITHNMDEAKYADVVYVMHKGTIT
                     LSGTPKEVFTNVDEMIEAGLDVPQSTRLLYELKKEGIKIENLTIEEDETADILGRLLQ
                     "
     gene            complement(5569..6081)
                     /locus_tag="PLQ68_03830"
     CDS             complement(5569..6081)
                     /locus_tag="PLQ68_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015924328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bL17 family ribosomal protein"
                     /protein_id="HXK71814.1"
                     /translation="MPSNRKLDRPTDQRIAILKNLVTGLIQNGKLETTEARGKEVKKI
                     ADSLIAKAVAECDNFTSKPQKVTRAKTHKGKKLTMTKESKTSKTDYEVIDRETVEEMV
                     TVDNASRLHARRQAFKWINKIDGVNVVNKLYDEIAPKLKGRDGGFVKIYKIGQRRGDA
                     AEMVLLEIIK"
     gene            complement(6094..7035)
                     /locus_tag="PLQ68_03835"
     CDS             complement(6094..7035)
                     /locus_tag="PLQ68_03835"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015360241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HXK71815.1"
                     /translation="MIEIEKPRIECVEVNDEYGKFVVEPLERGYGTTLGNSLRRILLS
                     SLPGAAATSIKIDGVLHEFSTIPGVVEDVTEIILNVKGIRLRLDSDEPKTLTIRTEGT
                     GDVTAGDILKNAEVEVLNPDHYLFKMDNCESFYMEITVAKGRGYDSAEKNKKEGMPIG
                     TIPVDSIYTPVNRVVYKVEDTRVGQITDYDMLTMEIYTDKSMSPTKALSYAAKIMSDH
                     LNLFIDLSEEVKQTETVVETEEQTKNDLFDKPIDELDLSVRSYNCLKRAGINTVGDLT
                     SKTEDEMMKVRNLGKKSFDEIRKKLDELGQTFSRPEE"
     gene            complement(7080..7706)
                     /gene="rpsD"
                     /locus_tag="PLQ68_03840"
     CDS             complement(7080..7706)
                     /gene="rpsD"
                     /locus_tag="PLQ68_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015924326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HXK71816.1"
                     /translation="MARYTDAVCKLCRREGVKLFLKGDRCYTDKCAIVRRAYAPGQHG
                     KKRSKQSEYGLQLREKQKARRFYGVLESQFLKYYDMASRQKGMTGENLLKILESRLDN
                     VVYRMGLANSRPEARQLVRHGHFNINGKTVDIPSYLVKVGDVIQLKDSMKDSPKFKEL
                     IETSQTKIVPAFIEFDQENVTGKIIDVCKREDVSLPIEERFIVEFYSK"
     gene            complement(7722..8123)
                     /gene="rpsK"
                     /locus_tag="PLQ68_03845"
     CDS             complement(7722..8123)
                     /gene="rpsK"
                     /locus_tag="PLQ68_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016205877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HXK71817.1"
                     /translation="MAQQKSAVKKSRKRKEKKNVEHGQAHIRSSFNNTIVTMSDLQGN
                     VLSWASAGGLGFRGSKKNTPFAAQVAAETAAKAAMEHGLRTVEVYIKGPGAGRESAIR
                     AMETVGLSVVMIKDVTPIPHNGCRPPKRRRV"
     gene            complement(8137..8508)
                     /gene="rpsM"
                     /locus_tag="PLQ68_03850"
     CDS             complement(8137..8508)
                     /gene="rpsM"
                     /locus_tag="PLQ68_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015310899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HXK71818.1"
                     /translation="MARIAGVDLPREKRVEIGLTYIYGIGLTSSQKIIEQAGINPDTR
                     VKDLTEAEIVKLRDLIEKEHIVEGDLRREVSFNIKRLIDIGSYRGRRHRMGLPVRGQR
                     TKTNARTRKGPSKSSIGGKKK"
     gene            complement(8615..8728)
                     /gene="rpmJ"
                     /locus_tag="PLQ68_03855"
     CDS             complement(8615..8728)
                     /gene="rpmJ"
                     /locus_tag="PLQ68_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HXK71819.1"
                     /translation="MKVKPSVKKRCEKCKIIRRKGRVMVICENPRHKQRQG"
     gene            complement(8742..8960)
                     /gene="infA"
                     /locus_tag="PLQ68_03860"
     CDS             complement(8742..8960)
                     /gene="infA"
                     /locus_tag="PLQ68_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008900989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HXK71820.1"
                     /translation="MLANDVIETEGVVIESLPNTMFKVELPNKHVILAHISGKLRMHY
                     IRILPGDKVKIQMSPYDLTKGRIVWRGK"
     gene            complement(8983..9648)
                     /locus_tag="PLQ68_03865"
     CDS             complement(8983..9648)
                     /locus_tag="PLQ68_03865"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011034079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HXK71821.1"
                     /translation="MIIIMLGAPASGKGTQSAVVAQKLGIPHISTGDIFRWNIANNTP
                     IGAEAKKYIDKGLLVPDELTINILRDRIEMEDCKDGFVLDGYPRTINQQESLDKMLED
                     CGKSIDMAINLVVTDEEVVRRISGRRMCKCGKAYHIVTYPSKKPDICDLCNGELYTRK
                     DDTVEVANTRLDTYKKQSEPIIGVYREKGILVDIDGHKPQDKVSEDVLYEIHKYSSKQ
                     GVV"
     gene            complement(9656..10939)
                     /gene="secY"
                     /locus_tag="PLQ68_03870"
     CDS             complement(9656..10939)
                     /gene="secY"
                     /locus_tag="PLQ68_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014968382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HXK71822.1"
                     /translation="MFQTIKNAWNIPELRKKILFTITMLLIYRIGCHLPVPGLIGAAF
                     EVLVEGNKLFGFMDMFSGGAFSNMSLFAMNIQPYINASIILNLLTVAIPALERLSKEG
                     EEGRKKITQYTRYLTIGLGLFQASMLFITLKNYDVLYDGGTIMSFLTVVLTFCAGTAV
                     VMWLGELITEKGIGNGISMIIFVGIISRIPAGAASLYANWQNGDLGSTAVETILLVVG
                     LLVVMLGMIVGTVYVSGATRKIPVNYAKKVVGRKMYGGQSTHIPMQVNMSGVMPIIFA
                     SSITTLPSIIINFVAPNTESAFLKALQRTDSWAFLAIYALLIFFFSFFYTMIAFNPID
                     VANNLKKNGGFIPGIRPGKPTSDYLSYVLNKITWFGGLFLALLATIPSILARLVGIQI
                     GFGGSALLIVVGVALETVKQMESQMLMRHYKGFLD"
     gene            complement(10939..11382)
                     /gene="rplO"
                     /locus_tag="PLQ68_03875"
     CDS             complement(10939..11382)
                     /gene="rplO"
                     /locus_tag="PLQ68_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HXK71823.1"
                     /translation="MKLNELAPAEGSKKKAFRKGRGHGSGNGKTAGRGANGQNSRSGG
                     GVRVGFEGGQMPLYRRMPKVGFNNKRFQDKYAEVNIEALNIFNDKELVDEVLLREAGL
                     VKGVCDGIVILGRGELTKNLTVRAERFTKSAAEKIQAAGGKAEVV"
     gene            complement(11396..11596)
                     /gene="rpmD"
                     /locus_tag="PLQ68_03880"
     CDS             complement(11396..11596)
                     /gene="rpmD"
                     /locus_tag="PLQ68_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015513745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HXK71824.1"
                     /translation="MAKKKAEQAKMLKITLVKSTNSSIKKHQATVEALGLRKIGQTVE
                     QKDNEKIRGMVQKVQHLVKVEE"
     gene            complement(11607..12110)
                     /gene="rpsE"
                     /locus_tag="PLQ68_03885"
     CDS             complement(11607..12110)
                     /gene="rpsE"
                     /locus_tag="PLQ68_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008982617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HXK71825.1"
                     /translation="MRNDFDRTPSEFKEKVVRIGRVAKVVKGGRNFRFSALVVVGDEN
                     GRVGVGMGKAAEVPESIRKGTDDAKKNLITVATDGTTVPHEIIGEYGAAKVLLKPAAP
                     GTGVIAGGAVRAVLDLAGIKDIRTKCLGSHNAVNVARATIDGLSRMMTAEDISKKRNM
                     SIEEILG"
     gene            complement(12129..12497)
                     /gene="rplR"
                     /locus_tag="PLQ68_03890"
     CDS             complement(12129..12497)
                     /gene="rplR"
                     /locus_tag="PLQ68_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004620841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HXK71826.1"
                     /translation="MIKNIDKNNARKKKHDRIRNHLSGTAEMPRLNVYKSLNNIYAQV
                     IDDTKGYTLVSASTMDPSLKGTLKNGSNLEAAKQVGTLVAKKALEKGIKSVIFDRGGY
                     VYHGKIKALADAAREAGLEF"
     gene            complement(12503..13042)
                     /gene="rplF"
                     /locus_tag="PLQ68_03895"
     CDS             complement(12503..13042)
                     /gene="rplF"
                     /locus_tag="PLQ68_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010966397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HXK71827.1"
                     /translation="MSRIGKLPITVPAGVTVDIAPDNTVTVKGPKGTLTQTFVKSITI
                     KKEDGVVTLERRSEEKRQKALHGLTRSLLNNMVQGVLNGFEKKLEINGVGYKAALDGT
                     KLVLSLGYSHPLEVLAPEGITFEVPTPTTILVKGYDKQAVGQIAAQIREFRKPEPYLG
                     KGVKYSDETIRRKEGKAGA"
     gene            complement(13121..13516)
                     /gene="rpsH"
                     /locus_tag="PLQ68_03900"
     CDS             complement(13121..13516)
                     /gene="rpsH"
                     /locus_tag="PLQ68_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HXK71828.1"
                     /translation="MQISDVIADMLTRIRNAGTAKHVKVDITASNLKRDIAKILLEEG
                     YIQDVENIEDDKQGVIRLTLKYQDKKSVITGIKRISKPGLRVYSNKDDLPKVLNGLGI
                     AIISTSKGIMTDKKARALGVGGEVLAFVW"
     gene            complement(13529..13714)
                     /locus_tag="PLQ68_03905"
     CDS             complement(13529..13714)
                     /locus_tag="PLQ68_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012065428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HXK71829.1"
                     /translation="MAKKSMILKQQKTQKFSTREYNRCQICGRPHAYLRKYGVCRLCF
                     RELAYKGQIPGIKKASW"
     gene            complement(13728..14267)
                     /gene="rplE"
                     /locus_tag="PLQ68_03910"
     CDS             complement(13728..14267)
                     /gene="rplE"
                     /locus_tag="PLQ68_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004620849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HXK71830.1"
                     /translation="MKLQQLYKEHAVPALKDKFQYKNVNQVPKLVKIVVSIGMGEAKD
                     NPKALDSAAGDLAIITGQKPVVTLAKKSVANFKLREGMKIGLKVTLRGERMYDFADKL
                     INISLPRVRDFRGLSTSGFDGRGNYAMGLKEQLIFPEIVYDNIDKLRGMNIVFVTTAK
                     TDEEARQLLAELGMPFKKA"
     gene            complement(14279..14605)
                     /gene="rplX"
                     /locus_tag="PLQ68_03915"
     CDS             complement(14279..14605)
                     /gene="rplX"
                     /locus_tag="PLQ68_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008710999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HXK71831.1"
                     /translation="MHIKKGDNVKIITGNDSGKSGKILEVNKNKGTVIVEKANVGKKH
                     VKPKKAGQPGGIVDFERPVNASNVMLVCPKCKEASKTKMTFDKDGNKQRTCKKCGAVI
                     DKVSAK"
     gene            complement(14622..14990)
                     /gene="rplN"
                     /locus_tag="PLQ68_03920"
     CDS             complement(14622..14990)
                     /gene="rplN"
                     /locus_tag="PLQ68_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015924311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HXK71832.1"
                     /translation="MIQAQTRLKAADNTGAKELQCFKVLGGTGRTYANIGDVIVCSVK
                     TASPGGMVKKGDVVRCVIVRSKKGVRRKDGSFIKFDENAAVIIKVDKEPVGTRIFGPV
                     ARELRDKEYMKIVSLAPEVL"
     gene            complement(14997..15254)
                     /gene="rpsQ"
                     /locus_tag="PLQ68_03925"
     CDS             complement(14997..15254)
                     /gene="rpsQ"
                     /locus_tag="PLQ68_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012738581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HXK71833.1"
                     /translation="MTTRNLRKTRVGKVVSDKMDKTIVVAVQDSVRHPLYKKIVKRTY
                     KLKAHDENNECVVGDRVKVMETRPLSREKRWRMISIVEKAR"
     gene            complement(15256..15453)
                     /gene="rpmC"
                     /locus_tag="PLQ68_03930"
     CDS             complement(15256..15453)
                     /gene="rpmC"
                     /locus_tag="PLQ68_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HXK71834.1"
                     /translation="MKANDIRKKTNEELAVELNELKSELFKLRFQYATNQLENPLKMK
                     SVKKDIARVKTILREREIKEI"
     gene            complement(15443..15871)
                     /gene="rplP"
                     /locus_tag="PLQ68_03935"
     CDS             complement(15443..15871)
                     /gene="rplP"
                     /locus_tag="PLQ68_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006873278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HXK71835.1"
                     /translation="MLIPKRVKYRRVHRGRMKGKALRGNTVNQGDYGLVAVEPGWIKS
                     NQIEAARVAMTRYIKRGGNVWVKIFPHKPVTKKPAETRMGSGKGSPEFWVAVVKPGRV
                     MFEIGGVSEEDAKEALRLASYKMPIKCKIMARESGETNEG"
     gene            complement(15871..16530)
                     /gene="rpsC"
                     /locus_tag="PLQ68_03940"
     CDS             complement(15871..16530)
                     /gene="rpsC"
                     /locus_tag="PLQ68_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004626694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HXK71836.1"
                     /translation="MGQKVNPHGLRVGIIKDWDTKWYANKRDFSKYLVEDNKIRKFIK
                     KKLYIAGVSRIEIERAANKIKINILAAKPGLIIGKGGAGVEQVRADIEKLTTKSVLVN
                     ITEVSHIDMDAQLVSENIAAQLEKRISFRKAMKQAMSRTMKQGALGIKTMVAGRLGGA
                     EIARTEHYHEGTIPLQTLRADIDYGFAEANTTYGRIGVKVWIYKGEVLPKKKENEGGI
                     Q"
     gene            complement(16541..16978)
                     /gene="rplV"
                     /locus_tag="PLQ68_03945"
     CDS             complement(16541..16978)
                     /gene="rplV"
                     /locus_tag="PLQ68_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HXK71837.1"
                     /translation="MEKTTEKKEKTFTRKCNKAPLLTKKQRKELGVGKDQGKASIDNI
                     SIPASKAKLVVDLIRGKDYETALAIVKNTTNFVTETLEKLLISAHANAVNNNNLDESK
                     LYVAEVYANQGTTLKRIKALSRGSAARIKKRTCHLTVVLKERV"
     gene            complement(16990..17271)
                     /gene="rpsS"
                     /locus_tag="PLQ68_03950"
     CDS             complement(16990..17271)
                     /gene="rpsS"
                     /locus_tag="PLQ68_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015924305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HXK71838.1"
                     /translation="MSRSLKKAPYVFPRLLAKIEEMNASNDKKVLKTWSRSSTIYPQM
                     VGHTIAVHDGKKHVPVYITEDMVGHKLGEFVVTRTYRGHNKGDKTTKVK"
     gene            complement(17284..18117)
                     /gene="rplB"
                     /locus_tag="PLQ68_03955"
     CDS             complement(17284..18117)
                     /gene="rplB"
                     /locus_tag="PLQ68_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020816386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HXK71839.1"
                     /translation="MPIKKYNPTSPARRFMTVSTFEEITKTTPEKSLVAPIKKKAGRN
                     NYGRITVRHQGGGVARKYRIIDFKREKDGVPARVASIEYDPNRSANIALLNYADGDKR
                     YIIAPNGLSVNDTVMSGPTADIRVGNALPLANIPVGTMIHSIELKPGKGAQMVRSAGN
                     TAQLMARESDFAQVRLPSGEVRMVSVDCKATIGQVGNIDHANIRIGKAGKKRYMGVRP
                     TVRGSVMNPVDHPHGGGEGKSPIGMPSPVTPWGKPTLGTKTRKKKKASDKMIVKRRNS
                     K"
     gene            complement(18129..18488)
                     /gene="rplW"
                     /locus_tag="PLQ68_03960"
     CDS             complement(18129..18488)
                     /gene="rplW"
                     /locus_tag="PLQ68_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010246819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HXK71840.1"
                     /translation="MKSYEDIIIRPYITERTNEQAMEGRYTFMVAKKSTKVEIKQAVE
                     KLFNVKVLKVNTVNYDGKEKRVGVHLGRTASFKKAIVSIDTNPQAESYLEKGGKVKTL
                     AKKYKTSIEEFGVTPRQ"
     gene            complement(18488..19111)
                     /gene="rplD"
                     /locus_tag="PLQ68_03965"
     CDS             complement(18488..19111)
                     /gene="rplD"
                     /locus_tag="PLQ68_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HXK71841.1"
                     /translation="MLKADIYNLKGDVVDTMSLNEEIFGIEVSESAMHSVVVQYLANG
                     RQGTQSAKTRAEVRGGKAKPFRQKGTGRARQGSLVGPHQTGGGVAFAPKPRSYRFSLN
                     KKLKRLALKSALSEKYGSGSLVILDNLKLDEIKTKKFAEILKNVKVDSSCLVVLSEKD
                     DVVIKSANNLPNVKTALTNTINVYDILKYDKFVVTKEAVEKIQEVYA"
     gene            complement(19124..19753)
                     /gene="rplC"
                     /locus_tag="PLQ68_03970"
     CDS             complement(19124..19753)
                     /gene="rplC"
                     /locus_tag="PLQ68_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015360271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HXK71842.1"
                     /translation="MKNFMLGKKAGMTQLFLEDGQAVPVTVIEAGPLTVVQVKTTEHD
                     GYNAVKVAFGDVKESRVNKPDKGLYAKADLTPKKYVREFIVESTDGYKVGDVITVDSL
                     NEGMLVDVIGVSKGKGYAGTIKRYGNTIGPMSHGSKYHRGLGAMAMHTDPGRVPKGKR
                     MPGHMGTDRVTVQNLEVMKIYSDRNAVLVKGAVPGPKGGLIMIQNAKNA"
     gene            complement(19813..20124)
                     /gene="rpsJ"
                     /locus_tag="PLQ68_03975"
     CDS             complement(19813..20124)
                     /gene="rpsJ"
                     /locus_tag="PLQ68_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HXK71843.1"
                     /translation="MASKQRIRIRLKAYDHELIDQSANKIVETAKRTGAKVSGPVPLP
                     TNKEIVTILRAPHKYKDSREQFEMRTHKRLIDILSPTPKTVDALMLLDLPAGVDIEIK
                     L"
     gene            complement(20489..20788)
                     /locus_tag="PLQ68_03980"
     CDS             complement(20489..20788)
                     /locus_tag="PLQ68_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015049592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HXK71844.1"
                     /translation="MYEIRFTASAERYFKKVKEKRLLNEYYNTIKKISINPYIGEMKK
                     GDLAGVYCYDIYYNRTNYEIAYMIYEQEKRMIVVVLAGTRENFYQELKRYIKDKQ"
     gene            complement(20792..21160)
                     /locus_tag="PLQ68_03985"
     CDS             complement(20792..21160)
                     /locus_tag="PLQ68_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003528372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HXK71845.1"
                     /translation="MNSYIKNNQKERKIISVSKKRQITIPLKYFNILGLSNEVECILQ
                     ENSIVIRPIRENVSDEFGEFILTELIEQGYSGQKLITKFKEARRKIRPAVEDLLSEAD
                     DIAKGHKKSSTLEDVFKQED"
     gene            complement(21611..21895)
                     /locus_tag="PLQ68_03990"
     CDS             complement(21611..21895)
                     /locus_tag="PLQ68_03990"
                     /inference="COORDINATES: protein motif:HMM:NF012899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK71846.1"
                     /translation="MNTDAVLIAKNGVTGVYDKDSNQCEDARRYKKVTFNELIERNLE
                     VADQSVFISARDFNLPFYVFDFKNKDCIKDICEGRDVGTYIAHEIPIEWY"
     gene            complement(21993..23227)
                     /locus_tag="PLQ68_03995"
                     /pseudo
     CDS             complement(21993..23227)
                     /locus_tag="PLQ68_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014355844.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
     gene            complement(23336..24142)
                     /locus_tag="PLQ68_04000"
     CDS             complement(23336..24142)
                     /locus_tag="PLQ68_04000"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HXK71847.1"
                     /translation="MNAVITGAAGGLGRAFAVECAKRGYNLILTDISKEGLKTLKDGL
                     LRRYDINVIYFTCDLTDEFQTDKFKEYLSIRNIYIDILFNVAGIDHEGAFATRSLEQI
                     NSIVRVNIEAMLRITYMMMDFKRKNEPFRIINVSSLASLFPMPLKAVYAASKRFILDF
                     SYALNEELNEKNIYVLSLCPGGLATTKNAIDGMAVQGFFGNATSNRLEVVARRTIDKS
                     LRKRRVYIPGILNRILSIAGRLLPVSVVVRVIHSRWKRALYSRIHLRETL"
     gene            complement(24139..24993)
                     /locus_tag="PLQ68_04005"
     CDS             complement(24139..24993)
                     /locus_tag="PLQ68_04005"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HXK71848.1"
                     /translation="MKNVIVTGATSGIGFAVCEALLSSNHKVIGIGRSAESCEKARRS
                     LLEKNTNADIIFFSADLMQLEEVKKISSEILEHISMHWNGKLDVLINNAGCVRSRYCT
                     TKEGYEHQFALNHLSSFLLTYYLINPLIKAQGRVVITSSESHKGIKMNWNDLMFTRFY
                     RPLRVYKQSKLANMLFAFGLNDRHSVYGLRAYCVDPGLVNTKIGEKQTYGLVNMVWSL
                     RKKKGVTPDVPANSYLYLVESDNHPEGLYIHKNKPKEYSSYVNRENADRLWKISEKLC
                     SIEFGRFQ"
     gene            complement(24990..25709)
                     /locus_tag="PLQ68_04010"
     CDS             complement(24990..25709)
                     /locus_tag="PLQ68_04010"
                     /inference="COORDINATES: protein motif:HMM:NF019618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HXK71849.1"
                     /translation="MNKKEFMDIMKNSLLESGISDIDDILNEYEEHFLFKMADGFSEE
                     EVSLKLGDPKELAKQFVQEAPVIKVKKKNRFLTGLGLGFLDIFVGCIFLALFIGVIAL
                     AAVAISSATAGVLLITKMNIASLLPVMPYKSAVMFSVGLIALAILVFITVVYGFLWFK
                     QLLVSYSRFHKNCMAKASNKAVYPAKATFLQVSGKLKRTLRGTILVSLVIFLVAFIGG
                     YAVSALEAKALEFWHVWEWFQ"
     gene            complement(25702..26022)
                     /locus_tag="PLQ68_04015"
     CDS             complement(25702..26022)
                     /locus_tag="PLQ68_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012157967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HXK71850.1"
                     /translation="MNIQYKKGVIELCIFSLLYKSDRYGYDISEYLSKHIDISDGTVY
                     PILRKLKSEGLVTTYLSEESGGPPRKYYKLTKTGQDAYLKDRQEWLDFVDAVHELIKE
                     AENE"
     gene            complement(26172..27059)
                     /locus_tag="PLQ68_04020"
     CDS             complement(26172..27059)
                     /locus_tag="PLQ68_04020"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HXK71851.1"
                     /translation="MSILNIGSMNIDYVYKVEHIVSEGETQNVSSVETKIGGKGLNQS
                     VAAAKAGSLVYHAGLIGNGGEILTDYLKESKVDISLIRKSDTNQGHALIQVDKQGKNS
                     ILVYGGSNRMITKDYVDEIFTEFDNNDFLMVNNEISELEYIIKKAYEKSIRVVLNASP
                     IDKSLLNMNLDMVTYLVINEIEGKALSNQREPYKILEAILKKHENIKIILTLGPDGSI
                     YSDKEKTIYQESYKVEAKDTTAAGDTFMGYFVAGLAKGLEIEKCLKSASMAAAITVTK
                     SGAAETIPDISIVQEKLQL"
     gene            complement(27162..28361)
                     /locus_tag="PLQ68_04025"
     CDS             complement(27162..28361)
                     /locus_tag="PLQ68_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015542516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXK71852.1"
                     /translation="MISSFKKYTHTLNACYIGYVVQAIINNFVPLLFLTFNSSYNIRL
                     DKIALLVSINFMVQITVDLIGAKFVDKIGYRLSAVIAHVFSTVGLVLLAFLPDIMSDP
                     YSGILISIIIYAIGGGIIEVIISPIAEACPTKNKQSVMSLLHSFYCWGHVLVVVLSTV
                     FFISFGIENWKYLSLFWALVPSFNAIYFLKVPIYELDKGENGYLRIRDLLKNKLFWLM
                     VILMITAGASEQAMSQWASAFAESGLNVSKTVGDLAGPLVFAVLMGISRVIFAKKGEK
                     WDLNRFMIICGILCLSGYLLASLSSSAIIGFIGCALCGFSVGIMWPGTFSLASKQIRY
                     GGTALFALLAFSGDLGCSLGPYVTGKVASVFNNDLKAGLLASIGFPILFVIFLLTDRK
                     NKAVKLD"
     gene            28547..29995
                     /locus_tag="PLQ68_04030"
     CDS             28547..29995
                     /locus_tag="PLQ68_04030"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HXK71853.1"
                     /translation="MLKLNREKLTDKIYACWIGKNIGGTMGVPYEGKTEMNDIEGYAT
                     PKGEAYPNDDLDLQLVWLSAMEVHGPYQLNSHVLGEHWLRCIPPHWNEYGICKANMEC
                     GILPPMSGELNNRWKHSNGAWIRSEIWACLAPGHPDIAIRYAIEDAMVDHGMGEGTYA
                     ELFTAAMESAAFVVQDIRELINIGLSKIPAECRIAKSVRLVLNAYDKRIDWKETRQLL
                     VEDSSDLGWFQAPANVGFVVLGLLYGNGDFKKSMIYAINCGDDTDCTGATVGSLLGIL
                     NGTAGIPADWREYIGEKIVTMAVNKAAFIYGWPDCPNNCKELTERTTRLIPGVFAANN
                     IQMEFTDSSDQYEEGQLENLKNRTYAETLCNRSGYSYDIDMLYALARVEFEDSPEIKP
                     FETKKVRITYFNKTQQSQNISFRFILPEGWKLEGNHAVMLESRGAALNMYTIWEATVT
                     AGEQTAHINRVIIEAASNSHLITRYIPILFIG"
     gene            complement(30036..30317)
                     /locus_tag="PLQ68_04035"
     CDS             complement(30036..30317)
                     /locus_tag="PLQ68_04035"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HXK71854.1"
                     /translation="MLRGIEHNKKKQIINFVYISICAIIILLFAINIILESDKKNISI
                     WAYDEVSAVVDFGIFVGNDKNMLLPGENFKYSEAVTAIRNLLMSAGLIN"
     gene            complement(30326..31147)
                     /locus_tag="PLQ68_04040"
     CDS             complement(30326..31147)
                     /locus_tag="PLQ68_04040"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HXK71855.1"
                     /translation="MDIGMVSRGFPNVTVEECSGYLKNFGFKTTELCFMFSNINTWAY
                     NGYKPMDDLTDELAANIVKIFRDNSIEIVSIGAFSSLFEPDSQKQADNFKAYERYIQI
                     ASNNNIKYISTETGFIPGRRGINADTYEKDFDYFKSNMIKLLDIAERYNVAVAFEPCI
                     LDLTPSAKRTRDFILQCERDNLKILLDPANLFANSDEEDMFKYLKDYVAYFHGKDRLV
                     NDAYGRNVGEGDIDWVKFLRLYKKYTPDTPFILEYCNKDNCETVKQRVLDYEKQV"
     gene            complement(31160..31705)
                     /locus_tag="PLQ68_04045"
     CDS             complement(31160..31705)
                     /locus_tag="PLQ68_04045"
                     /inference="COORDINATES: protein motif:HMM:TIGR04518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HXK71856.1"
                     /translation="MNNTKRIAIISLLTALTVILAKLLAINLQVIRIGVEFVSIALGS
                     ILFGPAWGALSAVLADILGGFLFPTGPYSPVISIVAIANGLIYGFYFYNKEITAKRTI
                     IAVLTKALVSDFILMSIALYTLYQIPLQELLLARSIKTAAVIAIETLIIYLVLRPIRN
                     ILYKKGWILPDSPQTEIIKKD"
     gene            complement(31702..>32292)
                     /locus_tag="PLQ68_04050"
     CDS             complement(31702..>32292)
                     /locus_tag="PLQ68_04050"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HXK71857.1"
                     /translation="GIEEHLYAFYDNPELMHRMNQDEVEYIKYVFEQMGEAIQPDFMT
                     FAEDMSYNLGAMLSEDMFDEFLLPYYKQTIPYIESIGTIPLVDTDGQLEGMIPWLQRA
                     GIRGALPLERKAGVDVNRIRENYPDFLVIGAYDKMVMDVSEEAMREEFERLLPAMKSG
                     GFIPSVDHQTPPGVSLENYKKYLSLLKEYCIKGAAR"
BASE COUNT         8999 a         6828 c         4958 g        11507 t
ORIGIN      
        1 tagttatatc acagtttttg tatgtaatat ttaataattt attttgtaac tgtagtctcg
       61 catttatata acccatcatc tgagacataa cttatggtag ctgtattagg actattacct
      121 acagcggtaa aattataata taattttagt ggttcatctc ctgttcttat acctgatata
      181 acgattttat tagtgtctga acttttaact gttccgtttg gtacctgaat atatttaccg
      241 tattgatcat aagcatatac gcaaacataa tcataataag ctgacatata tatactattc
      301 aaactcggag cttcgccacc aagtctgact cgtgttacat ttataacatt gtcattttta
      361 ccaataacct taatatttgc tgtagtatct aaaataatag gttttattac tgttatttca
      421 cctttttctt ctaatatttc tatattatta ccatcagact cataacaaaa agcatctggt
      481 agagaaacat catttgtaga ttgtaatagc acatttagtc cacttgctgt tactccactt
      541 attgatattt taagattatg ctcttctgat atagctcctg catacatatt gtgccttcca
      601 tattcgaaat ttaaccgaca tcctacaacc tctgagtcat ctactgcagt cagagtaaac
      661 tcatacacgg cttctatata gtttggattt gtagaaccat aagatccgcc cctacatgct
      721 attattctta gtgtctcagt cccagctctt tttgctgtaa cattaatata catcccattc
      781 gtttcatctc ttgtaatctc aaaaactggg gaatctccag aaatctcagt cccatatgga
      841 ttattatcat ccgttcttct tacctgaata taaaaataat taaaaccaag accgaaagta
      901 tttccttgac tatcttttgg atgtgttctt attttaagtg tttttccgac cagcaaccca
      961 ggacattcca gattataact tatcgatgaa aaagtattcc tttcactctt tccgtctaaa
     1021 gtaataggtg tgcttatatt ccataatcca tcatcagatt tgtaacttat aactgctgta
     1081 tttgtgccgg aagtaatgcc gtatgcatca caacaaacat accttaaagg atttacaccg
     1141 ggtctaggat cgcctaacac aatttgagaa gtatctgaac tagtaaaagt acctgatgga
     1201 ggtaatattt cctgaccata ttggtcagta gcatttacat ataaatatcc acctggatag
     1261 gcaaagatcc acattctggc tgcatatggt atgcttggat ccgtttctga actagctttc
     1321 acatctcttt gatcatttat aataccctgt tcatacaatg tatctatgaa taattttccc
     1381 tgcttattgt tcagcttcaa tgtttcctgt aaagttacgg cgatatcttc aattgtaata
     1441 taaccttctt ttacagttaa tccttttgat tcagcaaaag ccttgacatc agcccattca
     1501 aaatcgacac cttgcttgta acctagcaga tccagcatta tttttacaat ttcagttccg
     1561 cttatcatat ctgaaggcct gtaatttcca ttaccatcac cgataaaacc aagttccggg
     1621 tgagcatatg tatatgctaa tattggtttc caaaaagcag catttaaagg tgatatatca
     1681 ttaagattct ccgtgctatt aaacctcttc gcctgagact ccaagcctct taaccgcaag
     1741 tttatcagca ttccttgtgc ccttgtagca tttcttgata agtattcatc agtaatagtt
     1801 ccaccttctc ctcttataat accaatagct gcacaagctc tcgttgcatc ggtaattgct
     1861 gttgggatta ctattactgt tgttaacata gcaataacta gaattgatat cagtattctt
     1921 cttgctttca tgtgtttact ccttagttat atttaatatg taatatacta ttttttaata
     1981 atttataacg gcaaaatatg tctgttaaat attcatgtaa actgtgtatt ttcaatatac
     2041 tctttatcta atagtatgtc aaatttgttc ataaatagtt tacaatttca gaaaaacaca
     2101 ctttttctac ttattttacc cttacaccat atataacgta aaaaaggggt aaagggtgga
     2161 cagatttttg aaaatatttt taattcactt ctttttttat atattaatac atatattcat
     2221 acattcacat aagatttcta tttttgtttt aattatcttt cctatccttg tataatggat
     2281 gaagtagtgg taacaaggag gataagatga tattcaacct ttcaagacca aaatccatgc
     2341 ttccaaactc atacttctgg acatgggatc aatctacaaa ctgggtttta gatgatcctg
     2401 gtattgtaaa tttcggtagc tggaataaat acttgaaaaa tccagaaaca tatatagaag
     2461 actataaacg ccttactgat atggctcaaa gccttgggat aaaaggaata atcatctggg
     2521 gatttttaag agattcgcat ggtagtattg aatacgctaa aaaagtcgct tcctatgcaa
     2581 aatccaaggg agtgtctatc atgccgggag taggtactac tcattatggt ggtgtctatt
     2641 atgagggaaa taataaatac aacataaata cattcttgca tgctaatcct gaatgtggag
     2701 cagtagtatc taaagaacca ggtgcaaaaa gagcacatca tgcagtatgc ccaagcaacc
     2761 ctttatttaa agaatggata gatgaaggac ttcactggtt gttcaaggaa ttcgaaatag
     2821 gaggatgcaa ttttgaaaat ggtgatttct tctcctgtta ctgtgacaaa tgccaatcac
     2881 agaaagaaac ctggccaaag aatgatccta attttttcag atatcagtca atgtgttatg
     2941 aacctgcttt aacagccgtt gaaaaattcg ctaaagaaaa tgatgacctt ctaattactt
     3001 gggctactta ttgcggattt ctaccaggta atattaataa tatacaagat aagcctgaca
     3061 gctatattaa gaatatggga accgacagac cggaaatatt taacagaatg agttatgaag
     3121 gcatagccca gtggacatta accagaatgg tccgggaaaa agagatttca ctgatggact
     3181 ttctggatga tgggactcca agtcttgttt tcgataatcc ctcatggttg aaaggaatca
     3241 cccctccttc aaaaaggagc acaggttttg tacatcaggg aagccagtgg gattattcac
     3301 catgggagag caaatccaca agatacagcc tcatactcgg cagaataaaa gaagcctgct
     3361 taagagcata tgaatcaggt ctggagggtg tttcaataca tggggaactg acatcaagac
     3421 atatccctgc agctcttaat tatcttgctt tttctcattt cacgcactgg ccatatgata
     3481 ctttacgtgc attcggccaa aaaacactgg gagaggtctt caataatcca aaagaaggtg
     3541 aagattatgt ggaaatactt gccctatggg aagcaggaaa agttgatgaa cagctgaaaa
     3601 aaagaataaa ggaaagatat gattctgcct attcgattat ttatcctgca agtgaggatg
     3661 catcagaatc aaaacttacc agattcttct tctggaactg gttatacagg cttgtgtcaa
     3721 gttttcacgg aatagaagaa tctttcttct agatgaaaat aaaatgctat tgtatgttat
     3781 gcattttata aaatatatat atgaaattgt tggtaaaata aacttggttt atttcttaag
     3841 atgtttaatt atctcttctt tagcgtcaga tacagtgtat aatttgtcat ttatttcagg
     3901 atgagagatt ttaatctttt ttaatatttt tgttacttca ggcacatcaa gaccaaggct
     3961 tgtaagctgt tcaacatttc cgaaaacttc atctcttgta ccatccataa ctacctttcc
     4021 gtcatgtaat acaattatcc tttttgcata ctgtgcgaca agttccatgc tgtgagcaac
     4081 caggatgact gtacactctt tgttgtcatg aagattctgt ataatactga acagttctct
     4141 tgttccttca gggtctaatc ctgctgcagg ttcatcaaga acaagtattt caggttttag
     4201 tacaagaatg cctgcaattg ctgctctttt tttctggcct ccggaaaggt cataaggaga
     4261 agcattaagg ttttcctgag ttaatccaac catctttgct gtatccaaga ctctctgctt
     4321 tctctggtct tcaggtacat ttctctttat caatccaaac tcaatatcct tatatactgt
     4381 ctcttcaaaa agctgatact gcgcatactg gaaaaccagt cctacttttc ttcttaaatc
     4441 agactggact ttcttgtcct ttgtatctat tccatcaaca agaacttcac ctatgtcagg
     4501 tttcaataat ccgttcatat gttgtatgag agttgactta ccgctcccgg ttgctcctat
     4561 tatggctgta tattcattac tatagaaatc aagattaata tcatccaatg ctttctttga
     4621 aaaaggagtg ttttttgcat atgtatagca gacctgtttt aatgatatta ttctgtttgt
     4681 ttcatctgta ttcatattac tgtagcagcc ttcctaagat atcagcagtt tcatcttctt
     4741 caattgtcag gttttctatt tttattcctt cttttttcag ttcataaagt agtcgtgtag
     4801 actgaggaac atccaatcct gcttcaatca tctcatcaac atttgtaaaa acttctttag
     4861 gagtaccact aagagttatt gtgcctttat gcattacata aaccacatca gcatacttgg
     4921 cctcatccat attatgagtg atatttataa tgctaatgcc ttcttcttta ttaagtttct
     4981 cagtaacagc taaaagttct gcctgccctt tagggtccag catggatgtt gactcatcaa
     5041 ggattatgca cgaaggcttc attgcaagta cccctgcaat tgcaactctt tgtttctgtc
     5101 cccctgacaa ggtatgaacg gctctttctt tgagatcata tatccctatc tgattaagag
     5161 cattatctac agacttaatt attttttctc tttcataccc aagattctcc ggaccgaaag
     5221 ctacatcttc caacaccctg gtacctacaa tctgattatc aggatcttga aaaatcatac
     5281 ctattttctt tcgtatggaa agctcattct ttttttcctt gctgttcatc ccaagaacga
     5341 atatatcgcc agaatcagga agaataaggg catttagcat tctggcaaga gtagacttac
     5401 ctgaaccgtt tcttccaagt attgctatat acattccttt agatacagat aaggaaatac
     5461 cggaaactgc ttgtgttgtt tccggttgtg ttttgtatgc ataatagata tcttttactt
     5521 caatcatagt atagtagtaa aaaagccttc taagaaggct tatttttttt acttgattat
     5581 ctcaagcaag accatttcag cagcatctcc acgtctttga ccgatcttgt aaatttttac
     5641 aaatccacca tctctgcctt taagctttgg tgctatttca tcatatagtt tattaactac
     5701 attgactcca tcaatcttgt tgatccattt aaaagcctgt cttcttgcat gtaatctgga
     5761 agcattatca actgtaacca tttcttcaac agtttctctg tcaattactt cataatcagt
     5821 ttttgaagtc ttgctttctt ttgtcattgt aagctttttg cctttatgtg tctttgcacg
     5881 tgtgactttt tgaggttttg atgtgaaatt atcacattca gcaaccgctt tcgcaatgag
     5941 actgtcagct atctttttaa cttcttttcc tctggcttct gttgtttcaa gcttcccgtt
     6001 ttgtattaag cccgtaacca aatttttaag aatagctatt ctttggtctg tgggtctatc
     6061 aagttttctg tttgacggca tttttatacc tccttattct tctggtctgg aaaaagtctg
     6121 tcccagctca tcaagcttct tgcgtatttc atcaaatgat ttcttaccaa gattacgtac
     6181 tttcatcatt tcatcttcag tcttgcttgt aagatcacct acagtgttga taccggctct
     6241 cttaaggcaa ttgtatgatc tgactgaaag atccaattca tcaatcggtt tgtcgaacaa
     6301 atcgtttttg gtctgttctt ctgtttccac aacggtttca gtctgtttga cttcttccga
     6361 aagatcaata aacaggttaa gatgatcact cattatctta gctgcataac tcaaagcttt
     6421 agttggtgac atggacttat cagtatatat ttccattgtt aacatgtcat agtctgttat
     6481 ctgtcctaca cgagtatcct caactttata aaccactctg tttacagggg tatatattga
     6541 atcaactgga atagtaccta taggcatacc ttctttttta ttcttttcag cagaatcata
     6601 gcccctgccc ttagcaactg taatttccat gtagaaagat tcacagttat ccattttgaa
     6661 aagataatga tcaggattaa gaacttctac ttcagcattc ttaagaatgt cacctgcagt
     6721 aacatctcct gtaccttctg tcctaatggt caatgttttt ggttcatcag aatctagtct
     6781 aagcctgatt cctttaacat taagaattat ttcagtaaca tcttcaacta cgccgggaat
     6841 tgttgaaaac tcatgtagta cgccgtctat ctttatgctc gtagcagctg ctccgggcaa
     6901 agaggataac agaattcttc ttaaggagtt gcccaatgta gtgccatacc ctctctcaag
     6961 gggttcaact acaaatttac catactcatc gtttacttca acgcattcga ttcttggttt
     7021 ctctatttct atcaatgtta taccctcctt tttaatttaa tggtatatta tgctgattac
     7081 tacttagagt agaactcaac aataaatctt tcttctatag gcaggctcac atcttcgcgt
     7141 ttacatacat caatgatttt accagttaca ttttcttgat caaattcaat aaatgctgga
     7201 actatctttg tttgtgatgt ttcaatgagt tctttaaact taggagaatc tttcatagaa
     7261 tccttaagct gaataacgtc accgacttta acaagatatg aagggatatc aacagttttt
     7321 ccgttaatat tgaaatgtcc atgccttaca agctgtcttg cttcaggtct tgaattagct
     7381 aatcccatcc tgtaaacaac attatctaac ctgctctcaa gtattttgag aaggttttcg
     7441 cctgtcatgc ctttttgtct tgaagccatg tcataatatt taagaaactg tgattccaat
     7501 actccgtaaa atcttcttgc tttctgtttt tcgcgaagct gtaaaccgta ttcgctttgt
     7561 ttacttctct tcttgccatg ttgtccagga gcatatgctc ttctgactat ggcacattta
     7621 tcggtataac atctgtcacc ctttaaaaac agctttacgc cttcgcgtct gcaaagtttg
     7681 catactgcat ctgtatatct agccatttta tttgtacctc cttatactct tcttctcttc
     7741 ggcggtctgc agccgttatg tggaatagga gttacatcct ttatcattac aactgaaagc
     7801 cctacggttt ccatagccct gatggctgat tctctacccg cacccggacc cttgatgtaa
     7861 acttcaacag tccttaaacc atgttccata gcagctttag ctgctgtttc tgcagcaacc
     7921 tgtgcagcaa atggtgtgtt cttctttgaa cctctgaagc ctaatccgcc tgcgcttgcc
     7981 catgataaaa cattaccctg caaatcagac atagttacaa tagtgttatt gaaactggaa
     8041 cggatatgcg cctggccatg ttcaacgttc tttttttctt ttctttttct tgacttctta
     8101 acagctgatt tctgttgtgc cattgctcat acctccttac ttcttctttc cgccaataga
     8161 tgatttgctt gggcctttac gtgtccttgc attcgtcttt gtcctctgac ccctaacagg
     8221 caatcccatt ctgtgtcttc tgcctctata gctgcctata tcaatgagtc ttttaatgtt
     8281 gaatgaaact tctctacgaa gatcaccttc aactatgtgt tctttttcta taagatctct
     8341 aagtttaaca atttcagcct cagtaagatc ttttactcta gtgtcaggat ttattcctgc
     8401 ttgttctata atcttctggg aactggtcag tccgattcca tatatatatg tcagaccgat
     8461 ctctactctt ttttctctgg gtaaatcaac cccggcaata cgagccattt tactttttta
     8521 cctccatagt cctttcaaaa tctttcgagt gtctatgtta tcatcaccga aaacagtaat
     8581 taactgttca gccgcatcag taatgctaat cacattatcc ctgtctctgt ttgtgtcttg
     8641 ggttctcgca gataaccatt actctgcctt ttcttcttat tatcttacac ttttcacatc
     8701 tttttttaac tgatggttta actttcattt atattttcct cctatttgcc tctccatact
     8761 atccggcctt ttgtcagatc atatggcgac atctgtattt ttaccttatc tcctggaagt
     8821 atccttatat agtgcattct caactttcct gagatatggg ctaatataac atgtttatta
     8881 ggtaattcaa ccttgaacat tgtatttggt aaagattcta ttactactcc ttctgtctca
     8941 attacatcat ttgccaaaat tattgcctcc ttatccttag cattatacca caccttgttt
     9001 tgaagagtat ttatgtattt catataatac atcttcagaa actttatcct ggggtttatg
     9061 tccgtctata tcaacaagaa ttcctttttc cctgtacaca ccaattatgg gttcagactg
     9121 ttttttgtat gtatcaagtc tcgtgtttgc tacttcaact gtgtcatctt ttcttgtata
     9181 cagttcgccg ttgcacaaat cacatatgtc aggtttcttt gaaggatatg ttactatgtg
     9241 ataagcttta ccgcatttgc acattctcct gcctgatatc cttcttacga cttcttcatc
     9301 tgttacaacc agatttatag ccatatcgat agattttccg caatcttcaa gcattttgtc
     9361 taaggactcc tgctgattga ttgtccgagg atagccatca agtacgaatc cgtctttaca
     9421 gtcttccatt tcaattctgt ctcttaatat attgatggtc aattcatcag gaactaacaa
     9481 ccctttgtca atgtactttt ttgcctcagc tcctattgga gtattattgg ctatgttcca
     9541 cctgaatata tctcctgttg atatatgtgg tataccaagt ttttgtgcta ctactgcgct
     9601 ttgtgtgcct ttaccgcttg cgggggctcc tagcataata ataatcatga ctctcctaat
     9661 caaggaatcc cttgtaatgc ctcatcagca tttgtgattc catctgtttc actgtctcaa
     9721 gtgcaacacc gactacgatc agtaacgcgc ttcctccaaa accaatctga attccaacaa
     9781 gtcttgcaag aattgaaggt attgtagcta gtaaagccag gaacaagcct ccaaaccagg
     9841 taatcttgtt taatacatat gaaagataat ctgatgtagg ttttccagga cgtattccag
     9901 gtataaagcc accattcttc ttcaggttat ttgctacatc tattggatta aaagctatca
     9961 tagtgtagaa gaatgagaag aagaatataa gtaaggcata tattgcaagg aatgcccacg
    10021 aatccgttct ctgtaatgct tttaaaaacg cactttctgt gtttggtgca acaaaattaa
    10081 taattattga aggcaatgtt gtaatagatg aagcaaagat tattggcata acacctgaca
    10141 tatttacctg cataggtata tgtgtgcttt gtcctccgta catctttctt ccaacaactt
    10201 tctttgcgta gtttactggt atttttcttg tagctcctga aacataaact gttccaacta
    10261 tcataccaag cattactacc aaaagaccta ctactaaaag tatagtttct acggctgttg
    10321 atccaagatc tccattctgc caattggcat atagactagc tgctccagca ggtatcctag
    10381 atatgatacc aacgaagatt atcattgata ttccatttcc gattcctttt tctgttatga
    10441 gttcgcccaa ccacattact actgcagtac ctgcacagaa tgttaatact actgtcaaga
    10501 aactcattat tgttccgcca tcatacaaca catcgtaatt ctttaatgtt ataaacagca
    10561 tgctagcttg gaagagtccg aggccgattg taagatacct tgtgtactgg gtaatctttt
    10621 ttcttccttc ttcgccttct tttgatagcc tttcaagagc gggaattgca actgtaagaa
    10681 gatttaaaat aatggatgca ttaatgtatg gctgtatgtt cattgcaaac aatgacatat
    10741 tggaaaacgc tccgccggaa aacatatcca tgaatccaaa cagtttgttt ccttcaacca
    10801 acacttcaaa tgcagcacct attagccctg gtacaggaag atgacatcct atcctgtata
    10861 tcaggagcat tgttatagta aacaatattt tctttctcag ctcgggaata ttccaagcgt
    10921 tcttgatagt ctgaaacatt atactacctc tgcctttcct cctgcagctt gaattttttc
    10981 tgcagcggat ttcgtgaatc tttcagccct gactgtaaga ttcttagtta attctcctct
    11041 tcccaaaata actataccgt cacagacgcc tttaactagg cctgcttcgc gaagcaatac
    11101 ctcatcaaca agctctttgt cattaaaaat gttaagagct tcgatgttaa cttcagcata
    11161 tttatcctga aatctcttat tgttgaatcc aactttaggc attcttctat ataaaggcat
    11221 ctgaccgcct tcaaaaccaa ctcttactcc accgcctgaa cgggagtttt gtccgttagc
    11281 tcctctgcca gcagtctttc cattgcctga accatgtcca cggccttttc tgaatgcctt
    11341 tttctttgag ccttcggctg gtgctaattc gtttaatttc atttttagct cccccttatt
    11401 cttcaacttt tacgaggtgt tgtacttttt gtaccatacc tctgattttt tcgttatctt
    11461 tttgctctac ggtctgtcct atcttacgaa ggcctaatgc ttcaacagtt gcctgatgtt
    11521 tctttatgga actgttagtg ctcttaacca acgttatttt cagcatttta gcttgttcag
    11581 cttttttctt agccataaaa gccctcctaa cccaatatct cttcgatgga catattcctc
    11641 ttcttggaaa tatcctctgc ggtcatcatt ctagacaaac cgtcaattgt cgctctagct
    11701 acgtttactg cattatgtga tccaagacat tttgtcctga tatctttaat gcccgcaaga
    11761 tccaatacgg ctcttactgc accaccggca ataactccgg taccaggtgc tgctggctta
    11821 agtaatactt tagctgcgcc atattcgcct attatttcgt gaggaaccgt ggttccgtca
    11881 gtagctactg taatcaggtt tttcttggca tcatcagtac cttttcttat tgattcggga
    11941 acttcagctg cttttcccat accgacgcct acacgaccgt tttcgtcacc gacgacgacc
    12001 aaagcgctga aacggaaatt tctaccgcct ttaacaacct ttgctacacg accgatccta
    12061 actacttttt ctttgaattc cgatggtgtt ctatcaaaat cgtttctcat taaagcccgt
    12121 tcctcctatt aaaattccag accagcttct cttgctgcat cagcaagcgc cttgatcttt
    12181 ccatgatata catatccgcc tctgtcaaaa ataacacttt taatgccttt ttcaagagct
    12241 tttttagcaa ctagagttcc aacttgcttt gcggcttcta aattgctccc attcttaagg
    12301 gttcctttta aagaaggatc cattgtagaa gcagatacta atgtatatcc ctttgtatca
    12361 tctataacct gggcataaat gttatttaat gatttatata cattgagtct tggcatttca
    12421 gccgtgcctg aaagatgatt tctaattctg tcatgttttt tctttcttgc attattttta
    12481 tctatgttct taatcatatc tattacgctc ctgcttttcc ttctttacgt ctgatagtct
    12541 catcagagta tttaacaccc ttgcctaaat aaggttctgg ttttctaaac tccctaatct
    12601 gggctgcaat ctgtccgact gcctgcttat cataaccttt gaccaatatg gttgttggtg
    12661 ttggtacttc aaaggttatt ccctctgggg caagtacttc aagcggatgt gaatagccaa
    12721 gacttagtac taattttgta ccatctaaag ctgctttgta tcctactccg ttaatctcaa
    12781 gttttttctc aaaaccattg agtacaccct gtaccatgtt attcaagagg cttctggtta
    12841 atccatgtaa agccttctgt cttttttctt cacttcttct ttcaagtgta accacaccgt
    12901 cttctttctt tatggttata ctcttaacga atgtttgagt gagagttcct ttaggtcctt
    12961 taactgtgac tgtgttgtca ggtgctatgt ctactgtaac tccagctggg acagttatcg
    13021 gtaattttcc tatacgtgac atttatctaa ttcctcctgt tatgttctta atattgcgag
    13081 catctgccct tttcagaaac cacggatttt tatccgaagg ttaccaaaca aatgccagca
    13141 cctctccgcc tacgcctaaa gcgcgagctt tcttgtctgt cataatacct tttgaagtag
    13201 atattatggc gattcccaat ccgttaagta cttttggaag gtcatcttta ttagaatata
    13261 ctcttaaacc aggtttgctt attcttttaa tacctgtaat gacacttttt ttgtcctggt
    13321 acttaagtgt aagtcttata acaccttgct tatcatcttc gatattctca acatcctgta
    13381 tatatccttc ttctaagagt atctttgcga tgtctctttt aagattggaa gctgttatat
    13441 caacctttac gtgtttggct gttcctgcat tccttattct tgtaagcata tctgctatta
    13501 catctgaaat ctgcatgttg ctcctccttt accagctagc ttttttgata ccagggattt
    13561 ggcctttata tgctaattcc ctgaaacaca atctgcatac gccgtatttt cttaaatatg
    13621 catgaggtct tccacagatc tgacatctgt tgtactctct tgtgctgaac ttctgagttt
    13681 tctgctgttt taatatcatt gatttctttg ccaagatctt atcctcctta tgctttcttg
    13741 aaaggcatgc ccagttcagc aagcaattgc cttgcttctt catcagtttt tgcagttgtc
    13801 acgaaaacta tgttcattcc tctgagcttg tctatattat cataaactat ttctgggaat
    13861 atcagttgtt cttttaatcc catcgcatag tttcctctac catcaaagcc tgatgtgctg
    13921 agacctctga agtctctaac tcttggaagc gagatgttga taagcttgtc tgcgaaatca
    13981 tacattcttt cacctcttaa tgttactttt aggccaattt tcattccttc tctaagtttg
    14041 aagttagcaa cggatttttt agccagtgta actacaggtt tctgaccggt tattattgca
    14101 aggtcaccag ctgcagaatc aagagctttt gggttgtctt ttgcttctcc cataccgatg
    14161 cttaccacaa tcttaacaag cttaggtacc tgatttacat ttttatactg aaatttatct
    14221 ttcagtgcag gaactgcgtg ttctttatat aactgctgta atttcatctt ttatcctcct
    14281 acttcgcgct tactttatcg ataaccgcgc cgcatttctt acaagttctc tgcttattgc
    14341 cgtctttatc gaatgtcatt ttggtttttg aagcttcttt gcattttgga catacaagca
    14401 tcacatttga agcattcaca ggcctttcga aatctacgat tccaccaggc tgtccagctt
    14461 tttttggttt tacatgcttc ttgccaacat tggctttttc cactatgaca gtgcctttat
    14521 ttttgtttac ttcaagaatt tttccgctct taccagaatc attccctgtt attattttta
    14581 cgttatcacc ttttttaata tgcatcttct cagtctcctt tctatagcac ctcgggtgcg
    14641 agagagacta tcttcatata ttccttatct ctcagttctc ttgcaacggg tccgaagata
    14701 cgcgttccga ctggctcttt atctactttg attattacag ctgcattttc atcaaatttt
    14761 ataaaggatc cgtcttttct tcttacgccc ttcttgcttc ttactatgac gcatctgact
    14821 acatcaccct tcttaaccat accgccaggt gaagctgtct ttacactgca tactataaca
    14881 tcaccgatat tggcataagt tcttcctgtt ccgcctaata ccttgaagca ctgaagttct
    14941 tttgcgcctg tattatcagc tgcttttaat ctggtctgtg cttgaatcat agtcaattac
    15001 ctcgcttttt ctactataga aatcattctc cacctttttt ctctagaaag tggtcttgtt
    15061 tccattactt ttactctatc tccaacaacg cactcattgt tttcgtcatg agctttcagc
    15121 ttatatgtcc tcttaacgat cttcttatag agaggatgcc ttacgctgtc ctgaacagcg
    15181 acaacgatgg ttttatccat tttatcacta acaacttttc caactcttgt ctttcttaaa
    15241 tttctcgttg tcatcttata tctccttaat ctcgcgctct cttagtattg tctttactct
    15301 ggcaatatct tttttaacgc ttttcatctt caaaggattc tccaactgat tggtagcata
    15361 ttggaatctg agcttgaaaa gttctgattt gagttcattc aactcaactg ccagttcttc
    15421 gttagtcttt ttccttatat cattagcctt cattcgtctc accactttct ctagccatta
    15481 tcttgcactt gataggcatc ttgtaacttg ccagccttaa tgcttctttg gcgtcctctt
    15541 cagaaactcc tccgatttcg aacattaccc tgcctggttt tacaaccgct acccagaact
    15601 ctggagaacc ttttccacta cccatacggg tttcagcagg tttctttgtt actggcttgt
    15661 gagggaatat tttaacccat acatttccgc ctcttttgat gtaacgagtc atcgctacac
    15721 gggcagcctc tatctgatta ctctttatcc aacctggttc aactgctaca agaccataat
    15781 cgccctggtt aacggtattg cctcttaagg ctttaccctt cattcttccc ctgtggactc
    15841 ttctatattt gactctttta ggtataagca ttattgtata cctccttcgt tctctttctt
    15901 tttagggagg acttctcctt tatatatcca gacttttacg ccgattcttc catatgtggt
    15961 atttgcttct gcaaaaccat aatctatatc agctctaagt gtctgtagag gtattgtacc
    16021 ctcgtgataa tgctctgttc tggctatttc agctccgcca agtcttcctg caaccattgt
    16081 tttgattcca agagctccct gcttcatagt tcttgacata gcctgcttca tagcttttct
    16141 gaaagaaatt ctcttttcaa gctgcgctgc gatattttct gaaactaact gagcatccat
    16201 atctatatga cttacttcag tgatatttac aagtacgctt tttgtcgtaa gtttttcaat
    16261 atctgctctg acctgctcta ctccagctcc acctttaccg ataataaggc ctggttttgc
    16321 tgcaagaatg ttaattttaa ttttattagc agctctttct atttcaattc tggatacgcc
    16381 tgcgatgtat aatttctttt taatgaattt tctgatcttg ttatcttcta caagatactt
    16441 tgagaaatct cttttgttcg cataccattt ggtatcccag tcttttataa ttcctactct
    16501 aagtccgtga ggattaactt tctgtcccat gcttagcctc ctataccctt tctttcagaa
    16561 caaccgttaa atggcatgtt ctttttttga ttctggcggc gcttcctctt gaaagagctt
    16621 taatcctctt aagagttgtc ccctggttgg catatacttc tgcaacatac aatttgcttt
    16681 catcaagatt attgttattt acagcatttg catgtgccga aatcaaaagt ttttccaaag
    16741 tctcggttac aaaattagta gtattcttga ctattgcaag agctgtctcg taatctttgc
    16801 ctctgataag gtcaacaaca agttttgctt ttgaagctgg aatgctgatg ttatcaatac
    16861 ttgcttttcc ctgatctttt ccaacaccga gttcttttct ttgtttcttg gtcaaaagag
    16921 gagctttatt gcactttctg gtaaaagttt tttctttctt ttcagttgtt ttttccattt
    16981 tttcctccat tatttaacct ttgtggtctt gtcgccttta ttgtgtcctc tgtatgtcct
    17041 ggtcacgacg aattcgccaa gcttatgccc aaccatatcc tctgtgatat atacaggaac
    17101 atgctttttg ccgtcatgaa cagctattgt atgacctacc atctgagggt atattgttga
    17161 ggatcttgac catgtcttca atactttttt atcgttagac gcattcattt cttcgatctt
    17221 tgctaaaagt cttggaaata catatggcgc tttctttaat gatctgctca tgtcaattct
    17281 cctttacttg ctgtttcttc tcttaacaat catcttgtca gaagcttttt tcttttttct
    17341 tgtcttagtt cccaaagtag gtttgcccca aggtgtaaca ggacttggca tacctatagg
    17401 agactttcct tcaccaccac catgtggatg gtcaacagga ttcattactg aacctctgac
    17461 tgtaggtctt actcccatat atcttttctt gccagcttta cctattctga tgttagcatg
    17521 gtcaatgttt ccaacctggc cgatagtagc tttacagtct acacttacca ttcttacttc
    17581 tcctgaagga agtctgactt gtgcaaaatc actttctctt gccataagct gtgcagtgtt
    17641 tcctgctgat cttaccatct gagcaccttt tcctggttta agctctatgc tgtgaatcat
    17701 agtacctact ggaatattag caagcggtaa tgcatttcct actctaatat ctgctgtagg
    17761 tccagacatt actgtatcat taactgaaag gccgtttgga gctataatat atcttttatc
    17821 tccgtcagca tagttaagta atgctatgtt tgcgcttctg ttaggatcat attctatgga
    17881 agcaactctt gcaggtacac catctttttc tcttttgaaa tcaataattc tgtactttct
    17941 tgcaactccg ccgccctggt gtcttactgt tattctaccg taattgtttc ttccagcttt
    18001 tttcttaata ggtgcaacaa gtgatttttc cggagttgtt ttcgtaatct cctcaaatgt
    18061 tgaaactgtc atgaatcttc tcgccggtga agttgggtta tatttcttaa ttggcataat
    18121 ttactccttt actgacgtgg cgttacgcca aattcttcta tgcttgtctt atatttttta
    18181 gcaagcgtct taaccttgcc gcctttttcc aaataacttt cagcctgcgg gttagtgtct
    18241 attgatacga ttgctttctt aaaagaagca gttcttccta aatgtactcc aactctcttt
    18301 tctttgccat catagttcac tgtgttaact ttaagaacct tgacattgaa aagtttttct
    18361 acagcctgct tgatttcaac tttcgtagat ttcttagcta ccatgaaagt atatctacct
    18421 tccattgcct gctcgtttgt tctttccgta atatacggtc ttatgattat gtcttcataa
    18481 cttttcatta cgcatagacc tcctgaatct tttctaccgc ttcctttgta acaacaaatt
    18541 tatcatactt gagtatgtca taaacattga tggtatttgt taaagcggtt ttaacatttg
    18601 gcaggttatt tgctgatttg ataactacat catctttttc tgaaagaact actaaacaag
    18661 atgaatcaac ttttacattt ttcaatattt cagcaaattt ctttgtttta atttcatcta
    18721 atttaaggtt atcaagtata acaagtgatc ctgatccata tttttctgac aaagctgatt
    18781 ttaaggcaag tctcttaagc ttcttgttta gtgaaaatct ataagatctt ggctttggtg
    18841 caaaagcaac tccgccgcct gtttggtgag gtccaactaa tgatccctga cgagcacgac
    18901 ctgtgccttt ttgtctgaag ggttttgctt ttcctcctct aacttcggct ctggtctttg
    18961 cactttgtgt tccctgtctg ccgttagcaa ggtattggac aacgactgag tgcatagcgc
    19021 tctcacttac ttcgattcca aatatttcct cgtttaatga catagtgtcg acaacatcac
    19081 cttttaagtt atatatatct gcttttaaca ttgctgtgtc ctcctatgcg ttcttggcat
    19141 tctgaatcat tatcagtccg cctttaggtc ccggtacagc gccttttact aaaaccgcat
    19201 tacggtctga ataaatcttc atgacttcaa gattctgaac tgttaccctg tctgttccca
    19261 tatgtcctgg cattctcttt cccttaggta ctctacctgg gtcagtatgc attgccatag
    19321 ctcctaatcc acgatgatac tttgaaccgt ggctcattgg gccgattgtg tttccatatc
    19381 ttttgattgt tcctgcatat ccttttcctt ttgatacgcc gataacatca acaagcattc
    19441 cttcattgag tgaatcaaca gttatcacat caccaacttt ataaccgtca gtagattcaa
    19501 ctatgaactc tcttacatat ttctttggtg taaggtcagc ttttgcatat aggcccttat
    19561 ccggcttatt tactctgctt tcttttacat caccaaaagc tacctttaca gcattatatc
    19621 cgtcgtgctc tgttgtctta acttgcacca cggtcagagg gccagcctct attactgtta
    19681 caggcactgc ttgtccgtct tccaagaaca actgtgtcat ccctgctttc tttcctaaca
    19741 tgaaattctt cactttaaat atcctcctaa gttattggtt ccaaacgtat ttcatccgca
    19801 ggaacttgac ttttacaact taatttctat atccactcct gctggcaggt ctaaaagcat
    19861 taatgcatcg actgttttag gtgtggggct taatatatca ataagacgct tgtgcgttct
    19921 catttcaaac tgctctcttg agtctttgta cttgtgtggt gctcttagta ttgttactat
    19981 ctctttatta gtaggtagcg gtactggacc tgatactttt gcgcctgttc ttttcgcagt
    20041 ctctacgatt ttattcgctg actgatcgat taattcgtgg tcataagctt taagtcttat
    20101 tctgattctt tgtttactag ccattctagt agttcctcct taaattcttt taccattcct
    20161 attagcacat agtcccgggt gtatcgctta tccccatttt taatgcccgc gtgacacggg
    20221 ctaggacctc tgtctaatcg cttccgccca acctttaaga ttggtacttc tccccttaag
    20281 ttacccgcaa aaaatacggc aatctcaagg atcatggtac tgcagaatac cgcagcctat
    20341 gaattgtact atatcttttt ctcttttgca acactttttt ttagaaattt atcgctcgaa
    20401 tcgcaacttt taatattata tcattttatc agctgtaaag aaatcttttt agcagaaaat
    20461 ttttgcttaa cgaatggtat aagtgcaatt actgtttatc ttttatatat cttttcagtt
    20521 cctgataaaa attttcccgt gtccctgcca atactacaac aatcattctt ttttcttgct
    20581 catatatcat atatgcaatt tcataatttg tcctattgta atatatatca tagcaataaa
    20641 cacctgctaa atctcccttt ttcatttctc caatatatgg attaatgctt atttttttta
    20701 ttgtattata gtattcattc agcaaccttt tttctttaac tttcttaaaa tatctctctg
    20761 ctgatgctgt aaagcgaatt tcatacattt atcagtcctc ctgcttgaaa acatcttcca
    20821 gcgtgctgct tttcttgtgc cctttggcta tatcgtcagc ttcgcttaac agatcttcta
    20881 cagcaggtct tattttcctt ctcgcctcct taaattttgt tatcagtttt tgtcctgaat
    20941 aaccttgttc aattaattca gtcaaaatga attcaccaaa ttcatctgag acattttccc
    21001 taattggacg aattactatc gaattttcct gcagaatgca ttcgacttcg ttacttaaac
    21061 caagaatatt gaaatatttt agtggaatcg ttatctgtct tttctttgat acacttataa
    21121 tttttctttc tttttgatta ttttttatat aactattcat ttagtatctc ctcctactgc
    21181 aggttttgaa caaagaaata tgcattacca agaaatatgt aattctttgt tttcattata
    21241 tacgagaaaa aaatttattg caactaatct gcgaatttaa tcttaagttt aaagcccttt
    21301 tccatcttga gtgtattact ctgacaacta ctgatacagg gagaagcctc cctgttatat
    21361 tgaatattct gctacaaatg caacaagaaa cattgccagt aatattgaaa ttgatccaca
    21421 aagagatctt ttgagttttc tgttaacctg taaataagta agctaaactt caaaaccact
    21481 cataaaacaa tgctttcatg tatattttta tatcaaaaaa gtgagattaa aactgcgtaa
    21541 taaaaaatat tatgattatg tacttatttc ctatctttca aaaatagttt aatgctacat
    21601 ttaatatatt tcaataccac tctattggga tttcgtgtgc aatatatgta ccaacatccc
    21661 taccttcaca tatatctttt atacagtctt tatttttaaa gtcgaaaaca taaaatggca
    21721 gattgaaatc acgtgctgaa ataaacactg attggtcagc aacctcaaga ttcctctcaa
    21781 ttaactcatt aaatgtaacc tttttatatc ttctcgcatc ctcgcactga tttgaatctt
    21841 tgtcatagac accagtcaca ccattttttg ctattaaaac agcatcagta ttaatatcca
    21901 gagcttttta tacagaaaga tagtcatcgg caataaaagg ttgaccattt cctccaccaa
    21961 ccacaatccg cttaattgtc aaagtacctt aattatatat tcttattttc aacaattatt
    22021 tccatgacac gctgtctgta ctcttttgaa acagttacag taagattttc gtatgtaaaa
    22081 gtgtctttta ccttaggtat tacaccaatc tgatttataa cccatccgct gactttagtc
    22141 tcctcgtatt catcatcaag accaaataat ctcatcattt cacgagtatc aacattacag
    22201 cttattctgt atctataatc atcaagtttt acaaatttct cttctatctc atcatgctca
    22261 tcccatattt cacctacaag ttcttccaga atatcctcca tagtcagaat tccaactgtg
    22321 cctccatact catcgattgc tactgccata tgttctttct caacttgaaa tttctttaac
    22381 agttcactga ttttaatatg aggagtaaca aacaaaacag gtgagagaat atcttcaata
    22441 ttctttgctc tttgcgttaa actgaaaaaa tctctgatat gtatgaaacc gagcatattg
    22501 tcgattgaat ttttgtaaac tggaatacgg gaatatcctg tttgaaggaa taattcagtt
    22561 atctcatcaa ttgttgaatt aattgacaca gcaacaacat caacacgtgg agttataata
    22621 tcaattgcct ttatatcatt aaactcaata acattgttaa taagctgttt atcttctttg
    22681 tttattgcgc cttcttgctc tgcttcttct acaaacataa gcaattcgct ttcagtaatc
    22741 gcttgattat ctgtagattt gaatatcttc gaaagcaatt ttttccattt aaaaaaaacg
    22801 aaatttaatg gagccattaa gaaagtcagc actcttagta atcttgctga aaacatagca
    22861 acttctctgg agactcttta gcaaggcttt taggagatat ctctccaaat atcaatacca
    22921 ctaatgttat aacaacagta gcaatagtag ttcctttgct accaaaatat cttacaaata
    22981 aaactgtagc aattgcagtt aatgctatat ttactatatt attgccaatc aatattgttg
    23041 acagaagttt atcatagttg tcataaagaa caagcacaag atttgctctt tttgaaccct
    23101 tctctgccat accctttacc ctaattctgc tcaaagatga aaatgcagtt tctgaagcag
    23161 aaaagtatga agaaaaaacg atcaagattc caatgacaac tattgatttt atgctttcac
    23221 tatccatcca gacagaaatc cctttccaaa acgttttcgc tttataataa agaattatat
    23281 aaatcaaaag cagaaatatt gctcttatat aatactataa ataatcatac ttttattaaa
    23341 gcgtttcccg taaatgaatc cttgaatata aagctctttt ccatcttgaa tgtattactc
    23401 tgacaactac tgatacagga agaagtcttc ctgctatact gagtattctg ttaaggattc
    23461 caggtatata tactcttctt tttcttaatg atttatcaat tgttcttctt gctactactt
    23521 ccaacctgtt ggatgtagca tttccaaaga atccctgcac tgccatacca tcaatcgcat
    23581 tcttagttgt agcaagccct cctgggcata atgatagtac atatatgttt ttttcattca
    23641 gttcctcatt taatgcatat gaaaaatcca gaataaagcg tttagatgcc gcatatacag
    23701 cctttagggg cataggaaac aaagatgcca ggctagacac atttataatt ctgaatggct
    23761 cgttttttct tttgaaatcc atcatcatat atgtaatcct taacatagct tctatattaa
    23821 ctcttactat gctattaatc tgctcaagac ttcttgtggc aaaagctcct tcatggtcta
    23881 ttcccgctac attgaaaagt atatctatat atatattcct tatacttaaa tattccttaa
    23941 acttatccgt ctgaaattca tcggttaaat cgcatgtgaa ataaatcaca tttatatcat
    24001 atcttcttaa aagtccgtct tttaaagttt tcagcccttc ttttgaaata tctgtaagta
    24061 tcaggttata tcctcttttt gcacactcaa cggcaaaagc tctacccaaa ccgcccgctg
    24121 ctccggttat gactgcattc attggaacct cccaaattca atagaacata acttttctga
    24181 tattttccat agcctgtctg cattttccct gttcacatag gaactgtatt cttttggctt
    24241 atttttgtga atatataaac cttcaggatg attatcactt tcaactaaat agagatatga
    24301 atttgccgga acatcaggtg tcactccttt tttctttctt agtgaccata ccatgtttac
    24361 taaaccatat gtctgctttt cacctatttt cgtattaacc aatcctgggt cgacacagta
    24421 agctcttaat ccgtacacag aatgtctgtc attcagaccg aatgcgaaaa gcatatttgc
    24481 taattttgac tgtttgtata cccttaaagg cctgtaaaag cgggtaaaca ttagatcatt
    24541 ccaattcatc tttatcccct tatgagattc agatgaggtt ataactactc ttccttgtgc
    24601 cttaatcaaa ggattaatta aatagtatgt taagagaaaa gaactaagat ggttaagcgc
    24661 aaactgatgc tcatatcctt cttttgtcgt gcagtatctg cttcttacgc aacctgcatt
    24721 gtttatgagt acatccagtt ttccattcca atgcatactg atatgctcca atatttctga
    24781 tgatattttt tttacttcct ccaattgcat aaggtcagca ctaaagaaaa taatatctgc
    24841 attagtattt ttctctaata agcttcttct ggctttttca catgattcag cacttcttcc
    24901 tataccaatc actttgtgat ttgatgataa tagtgcttca caaactgcaa aacctatacc
    24961 tgatgtggca ccggtgacaa ttacgttttt cattggaacc attcccatac atgccagaat
    25021 tctaaagctt tagcctctaa tgctgataca gcatatccac cgataaaggc gacaagaaat
    25081 atcaccagtg acactaatat agttccacga agagttcttt taagttttcc gctaacttgt
    25141 aaaaatgttg ctttggcagg ataaactgct ttgttagaag cttttgccat acagttttta
    25201 tgaaatcttg aatatgaaac aagtagctgc ttgaaccata aaaatccata tacaacagtt
    25261 ataaatacta agatagcaag agcaataaga ccaactgaaa acattactgc gctcttatat
    25321 ggcattacag gtaacaatga agctatattc attttggtta taagaagaac acctgctgtt
    25381 gcagaagata tagcaacagc tgccaatgca ataaccccaa tgaataaagc caagaatatg
    25441 caccctacaa atatatccaa aaatccaaga ccaaggcctg ttaaaaatct gtttttcttt
    25501 ttaaccttta taacaggggc ctcctgcaca aactgcttag ccagttcttt cggatctcca
    25561 agttttaaag atacttcctc ttctgaaaac ccatcagcca ttttgaataa gaaatgttct
    25621 tcatattcat taagaatatc atctatatca ctaatccccg attctaaaag actgttcttc
    25681 attatatcca taaattcctt tttattcatt ctctgcctcc ttaatcaatt catgcactgc
    25741 atctacaaag tccagccatt cctgtctgtc ctttaaataa gcatcttgtc cagtctttgt
    25801 aagtttatag tattttcttg gaggacctcc gctttcctca ctaagataag ttgttacaag
    25861 tccctcgctt tttaattttc tcaatatggg atatacagtg ccatcagata tatctatatg
    25921 tttagacaga tattctgata tatcatatcc gtatctgtca ctcttataca acaaagagaa
    25981 tatgcacagt tctattaccc cttttttata ctgaatattc aaaaatacac ctccatcaat
    26041 actatactta ctttactatt gtataatata tagtagttgg atacatgtca ataccttttt
    26101 ataaactatt ttctataatc ttgattctag ctttagctct ttatatactt cataattaaa
    26161 tatagtccat attacagctg aagtttttcc tgcactattg agatatccgg tatagtttca
    26221 gcggctcctg attttgtcac agtaattgct gcagccatac ttgctgattt taggcacttt
    26281 tctatttcta atcctttagc taatccagca acaaagtatc ccataaacgt atcacctgct
    26341 gcggtagtat ctttcgcttc gactttatag ctttcctgat aaatcgtttt ttctttgtct
    26401 gagtatatag acccatcagg tcccagcgta agtattatct ttatattttc atgttttttt
    26461 aaaattgctt ccagtatttt atagggttct ctctgattag ataatgcttt accttctatt
    26521 tcgtttataa caaggtaagt taccatatct aaattcatat ttaatagtga tttatcaata
    26581 ggagaagcat taagcactac tcttatgctc ttttcataag cttttttaat aatatactct
    26641 aattcagata tttcgttatt aaccatcaga aagtcattgt tatcaaattc tgtaaatatc
    26701 tcatcaacat agtcttttgt tatcatcctg ttggaaccac cgtatacaag tatgctgttc
    26761 ttcccctgtt tgtctacttg aattaatgca tgtccctgat ttgtatcact ttttctaatc
    26821 aaagatatat caacttttga ttctttaaga taatctgtta gtatttcacc gccatttcca
    26881 ataaggccgg catgatagac aagacttcct gcttttgctg ctgcgacaga ttgattaaga
    26941 ccctttcctc cgattttagt ttcaacagag gacacattct gcgtttcacc ttcactaaca
    27001 atatgttcca ctttatatac ataatcgatg ttcatagaac cgatattgag tatgctcata
    27061 tcaaagcctc caaacaatat aatgattaat tactataata tcatatataa atatattact
    27121 aaatcgcaaa caaactgttc tgatcttata gcttttacca tctaatctag tttaacagct
    27181 ttattctttc tgtctgttaa aagaaatatg acaaataata ttggaaatcc aattgaagct
    27241 aacagtcccg ctttaagatc gttattaaac actgatgcta ctttgccggt cacatacgga
    27301 cctaaagaac agcccaaatc acctgaaaag gcaagtaatg cgaataatgc tgtaccgcca
    27361 taccttattt gttttgacgc taatgaaaat gttcccggcc acattatacc aacagagaaa
    27421 ccgcataatg cacaaccaat gaacccgata attgctgatg aggataaaga agccaaaaga
    27481 taaccgctaa gacataatat gccgcatatt atcataaatc tgttaagatc ccacttttcg
    27541 cctttttttg caaatataac tctcgatatt cccataagca ctgcaaaaac caaaggacca
    27601 gcaagatcgc caactgtttt tgatacattg agtcccgatt cagcaaatgc tgaagcccac
    27661 tggctcatag cctgttcgct ggcaccagct gtaatcatta gtatcaccat taaccagaac
    27721 agcttattct ttaaaagatc tcttatcctt aaataaccat tttctccttt atccagttca
    27781 taaattggta ctttcaaaaa atatatcgca ttaaaagaag ggaccaaagc ccagaacaag
    27841 gacagatact tccagttctc tataccgaaa gaaataaaga aaactgtgga aagcacgaca
    27901 accagcacat gaccccagca gtaaaatgaa tgaagaaggc tcataacgct ttgcttgttc
    27961 tttgtcggac aggcttctgc tataggagag attattactt ctataatccc tcctcctatt
    28021 gcataaataa taatcgatat gagtatcccg ctatatgggt cgctcataat atcaggcaaa
    28081 aatgctaata gtactaatcc tacagtagaa aaaacgtgag ctattacagc ggataatcta
    28141 taccctatct tatctacaaa cttagctcct attaaatcaa cagttatctg aaccataaaa
    28201 ttgattgaaa ctaagagtgc tatcttatca agtcgtatgt tgtagctgct gttaaaagtt
    28261 aaaaacagaa gaggaacaaa attattgata atcgcctgta ctacatatcc tatgtagcag
    28321 gcattcaatg tatgtgtata ttttttgaag gaactgatca taagcacctg ttatcactaa
    28381 ttattatttg ctattatacc attaaaatta aaaaataccc tctataaaat gtattactta
    28441 agcactcttg attttgttat ctatttggca gataatttat gtgtttctat atgcatactt
    28501 ctatatacaa gaatgtagcg aatgtcaacg aaaggacaat taagtaatgc tgaaacttaa
    28561 cagagaaaaa cttaccgata aaatctatgc ctgctggatt ggcaaaaaca tagggggaac
    28621 catgggagta ccctatgagg gaaagacaga aatgaatgat atcgaaggtt atgctacccc
    28681 caaaggagaa gcttatccca atgatgacct cgacttgcag ctggtctggc tctctgctat
    28741 ggaagttcat ggaccttacc aacttaattc ccatgttctg ggtgagcatt ggctgcgttg
    28801 cattcctcct cattggaacg aatatggaat ctgtaaagca aacatggaat gcggaatcct
    28861 tcctcctatg tcgggtgaac tgaataaccg ctggaagcat tccaatggcg catggatacg
    28921 ttctgaaatt tgggcttgtt tagcaccggg acatcctgat atagcaatta gatatgctat
    28981 tgaagatgca atggtggatc atggcatggg tgaaggaaca tatgctgaat tgttcacagc
    29041 agcaatggaa agtgcagctt ttgttgtaca ggatataagg gaactgatta atattggatt
    29101 atctaaaatt cctgctgaat gtcgtatagc aaaaagtgta cgactggtat tgaacgcata
    29161 tgacaaaaga atagactgga aagaaaccag acaattgctg gtggaagaca gttctgatct
    29221 gggttggttt caagctcctg ccaatgtagg atttgtggta ctaggtcttt tatatggaaa
    29281 cggagatttt aaaaaaagta tgatatatgc cataaattgt ggtgatgata ccgattgcac
    29341 tggggctact gttggctcat tgctgggtat actcaatggt actgctggca ttccagccga
    29401 ttggcgagaa tacataggag aaaaaattgt taccatggct gtcaataaag ctgcatttat
    29461 ctatgggtgg ccggattgtc caaataactg caaagaacta actgaacgca caacacgttt
    29521 gattccagga gtatttgctg ccaataatat acagatggag tttaccgact catctgacca
    29581 atacgaagaa ggacaattag aaaatttaaa gaacagaact tatgctgaaa ccctctgcaa
    29641 ccgaagcgga tattcatatg acatcgatat gttgtatgcc cttgccagag tagaatttga
    29701 agattctcct gaaataaaac cctttgaaac caaaaaggtc agaattactt actttaacaa
    29761 aacacaacaa agccagaata tatcattccg ttttatcctg ccagaaggct ggaagcttga
    29821 aggtaatcat gcagtaatgc tggaatctcg aggagcagct ttaaatatgt ataccatatg
    29881 ggaagcaact gtaaccgcag gagagcagac agctcatatt aaccgtgtga ttattgaagc
    29941 agccagtaat tctcatttaa ttactcggta tattccaatc ctctttatcg ggtaaattag
    30001 acagaataaa aatgtctttt aactttttat gccttctaat ttatcaatcc tgcactcatt
    30061 aataaattcc ttattgctgt cactgcttct gaatacttga aattctcgcc tggcagaagc
    30121 atgtttttat catttcccac gaatattccg aaatccacaa ctgcagatac ttcatcataa
    30181 gcccatattg aaatgttctt tttgtcagat tctaatatta tattaattgc aaacaggagt
    30241 atgattattg cacatataga aatatataca aaatttataa tctgtttctt tttattatgt
    30301 tctatacccc ttagcattac ttttcctata cttgcttttc gtaatccagc accctctgct
    30361 tcacagtttc acaattatct ttattacagt attccagtat aaacggggta tcaggagtat
    30421 attttttata cagccttaaa aacttaaccc agtctatatc gccttctccc acattcctgc
    30481 catatgcatc atttactaac ctgtcttttc cgtgaaaata tgctacataa tcctttagat
    30541 atttaaacat atcctcttca tcagaattgg caaaaagatt tgcaggatct aaaagaattt
    30601 tcaaattatc cctttcacac tgcaagataa agtccctggt tctttttgca gatggtgtta
    30661 aatctaatat gcagggttca aatgcaacag caacattgta tctttcggcg atgtctaaaa
    30721 gtttaatcat attggattta aagtaatcaa aatccttttc gtatgtatct gcattgattc
    30781 ctcttcttcc aggaataaat cctgtttcag tggatatgta tttaatattg ttatttgaag
    30841 ctatctgaat atatctttca taagctttga agttatctgc ttgtttctgt gaatcaggct
    30901 caaataatga ggaaaatgct cctatggaaa ctatctcaat tgagttatct ctaaaaatct
    30961 ttacaatgtt tgctgccagc tcatcagtca ggtcatccat aggcttatat ccgttatatg
    31021 cccaagtatt aatgttgcta aacataaaac acaattccgt tgttttgaaa ccgaaatttt
    31081 tcaaatatcc tgaacattct tccacagtga catttgggaa cccccttgaa accattccaa
    31141 tatccatagc gatatctcct taatcttttt taataatctc cgtctgagga ctatcgggta
    31201 aaatccaacc ttttttatat aatatatttc taattggtct taaaactaga tagatgataa
    31261 gagtttctat tgctattact gctgctgttt taatacttct tgccaataaa agttcctgta
    31321 aaggaatctg ataaagtgta tacaaagcaa tgctcattaa tatgaaatca ctaactaagg
    31381 ctttagtaag tactgcaatt atggttctct ttgcagttat ttctttgttg taaaaataaa
    31441 atccgtaaat taaaccatta gctatagcta ctattgatat tactggcgaa taaggtcctg
    31501 tcggaaacaa aaagccacct aatatatctg ctaaaacggc agaaagagca ccccacgcag
    31561 gaccgaaaag tatagaccca agcgcaatgg aaacaaactc cactccaatt cttataacct
    31621 gcaaattaat agctaaaagt tttgctaaaa ttacagttaa cgctgttaat agtgatatta
    31681 ttgcaattct ttttgtgtta ttcaacgagc agctcctttt atacaatatt ccttaagtaa
    31741 agacagatac tttttgtaat tttccagtga tactccagga ggagtctgat gatctacact
    31801 aggaataaac ccgcctgatt tcatagcagg taatagcctt tcaaattctt cacgcatagc
    31861 ttcttctgaa acatccatta ccattttgtc ataagcacct attactaaga aatccggata
    31921 attttctctt atcctgttaa catcaactcc tgcttttctt tccagtggta aagcacctct
    31981 tataccagct ctttgaagcc agggaatcat tccttcaagc tgtccatcag tgtctactaa
    32041 aggtattgta cctatacttt caatgtatgg tattgtctgc ttataatatg ggagtaaaaa
    32101 ttcatcaaac atatcttcag aaagcatggc gcccaaatta tatgacatat cttcggcaaa
    32161 tgtcataaag tccggttgta ttgcttctcc catctgttca aaaacatact taatatactc
    32221 cacttcatcc tggttcattc tgtgcattag ttctggattg tcatagaaag catacagatg
    32281 ctcttctata cc
//
LOCUS       DAOSXM010000017        31158 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2111
            NODE_928_length_31158_cov_11.882809, whole genome shotgun sequence.
ACCESSION   DAOSXM010000017 DAOSXM010000000
VERSION     DAOSXM010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864178
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 31158)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31158)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:24:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,261
            CDSs (total)                      :: 2,229
            Genes (coding)                    :: 2,218
            CDSs (with protein)               :: 2,218
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 3 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31158
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_928_length_31158_cov_11.882809"
                     /isolate="AMR_MDS_2111"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            213..989
                     /locus_tag="PLQ68_04055"
     CDS             213..989
                     /locus_tag="PLQ68_04055"
                     /inference="COORDINATES: protein motif:HMM:NF024231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HXK71858.1"
                     /translation="MKTRKIAIAGIMGALCIVMALTPIGYIPIGPTKATIIHIPVIVA
                     AILEGPLVGAIVGLIFGLSSILNALLFPTVTSYVFLNPLVSVVPRILVGLVAYLVFAL
                     FKKMKKPLSVTLLTLMLFAIAYYLLNQSISNFQSGNIFYAIFNVFLLLVVFGAEIYTI
                     LNYESKNFAVALSAALATLTNTVLVLTMIGVFYGGEWVAKLGVEPSMARDFLVGIGVA
                     NGIPETVAAVIIGSVVVEALMKLVYKKNKPIEVPNNLKTE"
     gene            complement(1056..2051)
                     /locus_tag="PLQ68_04060"
     CDS             complement(1056..2051)
                     /locus_tag="PLQ68_04060"
                     /inference="COORDINATES: protein motif:HMM:NF012606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylinositol-specific phospholipase C
                     domain-containing protein"
                     /protein_id="HXK71859.1"
                     /translation="MKKLFAVLIISVMLLSMSGCQNILVNHDIPFYSEWMSYIKDDTL
                     IKEIVMPGSHDAGTIGMIYAAKTQSLPIHKQLIDGVRYFDIRVEKKKNAELVIYHSIA
                     KGQTFTAVIDDILEFMETNTTELIILDFQHFKNDSMEDVAAVIETNLSDYLIYNETDL
                     TDLDFIDNIPLGSVRGKILICWGSDVCAERSWAFRRNNDSGSLDNTVLDSYYDSAYHK
                     QDSEDFIANAYPDYYERYMKKDKGLFVLQGQLTAKWVLGSPLGREVEHRDNMNQFVIS
                     LKDNEQWLSYTNIVMRDFINVVWDKIDTVLELNEYKGNIKEGMDFLSNLESLKSD"
     gene            complement(2170..3834)
                     /locus_tag="PLQ68_04065"
     CDS             complement(2170..3834)
                     /locus_tag="PLQ68_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004611077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HXK71860.1"
                     /translation="MNKLKPKLFSVMKQYTKKQFFKDVIAGIIVAIIALPLSIALAIA
                     SGVSPERGIYTAIVAGFVVSFLGGSRVQITGPTAAFATIVAGIVARNGMDGLVLATLM
                     AGIILVIMGLLRFGSLLRYIPKTITTGFTAGIAVTIIIGQFKDFLGLTFNSKPVETID
                     KLIECFKSAGSLNWQAALVGVICLAILIFWPKISGKIPGSLIAVVVSSIIVKVFGMDV
                     NTIGSLYDISNQLPTFSLPVISISSVGSLLPDAITIAILAGVESLLSCVVSDGMIGSR
                     HRSNMELVAQGAGNVASALFGGIPATGAIARTAANVKNGGRTPIAGMVHSVVLVLVLV
                     VLMPYAAMIPMPAIAAILFIVAYNMSEWREVVSIVKTAPKSDILVLLVTFILTVVFDL
                     VIAIEAGIVISAFLFMKRMADETRIKSWKYIDIDRDEIENDKDSIALKVVPKHTMVYE
                     ISGPLFFAATQQFMNITTDPKLCSIILRMRSVPAMDITALDSLKKVHDRCLKNNITLI
                     FSHVQSQPMEMMKKAGFYNAVGMENFCPNIDTALERASLLLAERQS"
     gene            4549..5817
                     /gene="murA"
                     /locus_tag="PLQ68_04070"
     CDS             4549..5817
                     /gene="murA"
                     /locus_tag="PLQ68_04070"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015360377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HXK71861.1"
                     /translation="MSTEDRITIKGGKKLEGNVVVGGSKNAAVAIICASAMIKGKVIL
                     ENMPKITDIQVLLDTLSQIGAVVNWTGEKTVEVDATDIKSHICDNTALSTLRGSYYFV
                     GALLGRLKQAKVVFPGGCNFAPRPIDQHVYAFETLGATVETTGGYISAKARKLVGNNI
                     YFDNYSVGATINSILTATLAEGTTTIENANREPHVVDVANFLNIMGADIKGAGTNTIR
                     IKGVKELLGGKSYSVCPDMIEAGTLMIAAAATRGTITLNNVTPEHMDSLSAKLTEMGV
                     TVETMDDSITVKANIKRVKSTNIKTMPYPGFPTDLHPQFATLLAIADGESRITEGVFP
                     TRFQYVEQLHKLGANIQLSGESAIIKGVPKLIGAPVSPTDLRAAAAMVIAGLIAEGQT
                     TIFNLEYLDRGYEAFIEKLKSLKAVIERGK"
     gene            5819..6874
                     /locus_tag="PLQ68_04075"
     CDS             5819..6874
                     /locus_tag="PLQ68_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HXK71862.1"
                     /translation="MKHVDMIRDLTNANGISGFEEEVVGVIKKYAVSEDISVDTMNNV
                     FLTKKQNTNDRPVIMIDGHSDEVGFMVQFITANGLLKFIPIGGWQDANIAAQKVRIRN
                     KDGKYIKGVVASVPPHFLSAAQKDAPLKIENMLIDVGATSRDEVINDFGIEVAAPVVP
                     DVEFTINDNGIMCAKAFDDRIGAAAVIATMEELADESLDIDLIGTVSTQEEVGLRGAE
                     VTVKTVKPAIAIVYEGTPADDTFTNQYETQAALKKGPQLRHRDGSMITNPKFLAFVRQ
                     VAKENNIPFQDAVRSGGGTDGGKIHLVSGAIPTIVIGMPVRYAHTHYGYCAYDDFKNS
                     VELGKAILRKLTKDLKY"
     gene            7180..8643
                     /gene="malQ"
                     /locus_tag="PLQ68_04080"
     CDS             7180..8643
                     /gene="malQ"
                     /locus_tag="PLQ68_04080"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006427880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HXK71863.1"
                     /translation="MRASGILLPIFSLPSPYGIGTIGKEAYEFVDFLVSANQRYWQIL
                     PLGPTGYGDSPYQSFCSFACNSYLIDLDILEEKHLLQKDEYINESWYAQEDKVDYGLM
                     YQKRNAALEKAFKRFDVNNIKFKAYCQENSYWINDYAIFMALKEKFNGDKWQLWPDDI
                     RLRYHQSLMNYGTMLADRILFHKFLQYEFFAQWAELKKYANNKGIYIIGDIPIYVSSD
                     SSDVWSRHYLFQTDENRNLASVAGVPPDAFSATGQLWGNPLYDWTSMEKDDFYWWKQR
                     IGMASNLYDYVRIDHFIGIERYFSIPSDAETAASGTYYQGPGMKLIDAINLSRNNMGI
                     IAEDLGVLTDNVRKLLEKTGYPGMKVLQFGFSGEPANDNLPMHYKKNLIAYTATHDND
                     TTKGWYRKLDRKNRDFMKKYLNRSRIHVHDLILETMKSVADTAIIPMQDWLCQGSESR
                     INFPSVAFGNWTYRISKNMLTDNLASEISAMTVLCAR"
     gene            complement(8663..10348)
                     /locus_tag="PLQ68_04085"
     CDS             complement(8663..10348)
                     /locus_tag="PLQ68_04085"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019278418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular transferase
                     activity, phosphotransferases [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HXK71864.1"
                     /translation="MTYLDKYNEWLEKADLETVKELEALKGNEKEIEERFYKTLDFGT
                     AGLRGIMGAGTNRMNKYVIGQATQALASYVVEHGMEKRGAVIAFDCRIKSDEYAAQCA
                     SILAANGIKVYIYDSLRPTPQLSYSIRKLNAATGINVTASHNPKQYNGYKVYWEEGSQ
                     ILDDIADSIAANISKIDPFNDVKTVDFNEALKSGQITMLGKEMDDMFIKEILSLTLND
                     DIDKDINIVYTPLYGTGLVPVTRILKERGFHNVTIVEEHKNPDGTFPTTPYPNPENVK
                     VFDIPLQYGKRTDADMIIATDPDCDRIAMMIKDDKEEYVFCTGNQTGALLAEYMLSQR
                     QIKGMYSKKDAVVQSIVSGSIVPRIAKHYGTHFFETLTGFKYICGLANEFEKTDEYRY
                     VFGYEESIGYCIETFTRDKDGVSAAMMMCEMAGYYKKQGMTLYQVLNNIYEKYGYMSE
                     NQISIEYPGIKGAKTKKAVMSRWRKKPLVKINDIKCSVCKDYINGYEGLPEQDALKYE
                     FEDGSWYALRPSGTEPKLKVYINAMGCTKEEADDVLKAFTKDIKEYLVKCENK"
     gene            complement(10345..11373)
                     /locus_tag="PLQ68_04090"
     CDS             complement(10345..11373)
                     /locus_tag="PLQ68_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HXK71865.1"
                     /translation="MGKIVTFGEIMLRLKSPYGERLMQSDKLEATFGGGEANVAVSCA
                     NFGMDTSYVTVLPDNDIAKSCVRQLRGFGVNVDDIHYRNGRMGIYFLEPGAVQRPSKV
                     IYDREYSAISLTKPGDIDWLKIFENASWFHVTGITPALTQNSALVTLEAVTAAKQAGL
                     TVSMDLNYRGNLWKYGKKAAEIMPSIVKYVDVCIANEEDVQKALDIHNDADVTKGALD
                     ENKYKELSDKVLKTYPNIKLIAITLRESRNADINGWGACLNDRKDFYVSRKYEITDIV
                     DRVGGGDSFAAGLIYGLNTYGNSKDALEFAVAASCLKHTIYGDFSRFTVPEVEKLMRG
                     DGSGRVQR"
     gene            complement(11366..12019)
                     /locus_tag="PLQ68_04095"
     CDS             complement(11366..12019)
                     /locus_tag="PLQ68_04095"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018704257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-keto-4-hydroxyglutarate
                     aldolase/2-keto-3-deoxy-6-phosphogluconate aldolase"
                     /protein_id="HXK71866.1"
                     /translation="MRKEQVIENVMKSGLIVVVRAQSAEEAYRITDACLEGGVKLIEI
                     TFTVKGAHHVIEELSKKYDEKDLILGAGTVMDEQTARTAILSGAQYIIAPHLDIPTVT
                     MCHRYRVVAMPGCMTPREISMATEAGVDIVKVFPGEVLGTQFIKAVKGPMPYLNLVVN
                     GGVDENNAKDWIKAGACGVGVGGNITKYAKTGEYHKITATAKQILNSIKEAKEALNG"
     gene            complement(12081..12701)
                     /locus_tag="PLQ68_04100"
     CDS             complement(12081..12701)
                     /locus_tag="PLQ68_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008768185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HXK71867.1"
                     /translation="MKRESLEVFEEMLTSYPSLVRCKADIMRAFEIMEETYKYGKILL
                     ICGNGGSSSDAEHIVGELMKGFKKKRHVEPNPRLDESIRENLSEGLPAICLSSHTSLI
                     TAIGNDSSYDMIYAQQAYAYRKLAGAILCLSTSGNARNVNNAAITARACRIPVIGMTG
                     ELGGYLNSLADVCIKVPESETYKVQELHLPVYHCLCAMLECEFFEN"
     gene            complement(12719..13708)
                     /locus_tag="PLQ68_04105"
     CDS             complement(12719..13708)
                     /locus_tag="PLQ68_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016206677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HXK71868.1"
                     /translation="MYIAGIDIGGTKCAAVLGKCVDNNIIRVDIVDKVTFKTHSCNGP
                     SAVIDRLILGLEDILAKNFLISNDISCIGISCGGPLNSEKGLILSPPNLIGWDNIPIT
                     DIFEKHFNVKAVLQNDANAGALAEWFWGAGFGYRNFIFCTFGTGFGAGLILNGKLYTG
                     TNDMAGEIGHVRLSEYGPVGYGKMGSVEGFCSGGGISQLAKAMVKEEMQSGNKVSFCT
                     TEEDVEQLDALIVAKAAAKGDNLAKAIYARSGEMLGRALAVLIDILNPDAIAIGSIFT
                     KDRNLLWPHAKRVLNKESLAISSQVCDILPSSLGESIGDYEALAVAAEYVANN"
     gene            13958..15292
                     /locus_tag="PLQ68_04110"
     CDS             13958..15292
                     /locus_tag="PLQ68_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007495819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HXK71869.1"
                     /translation="MKDPILGPLILQILLISINAFFASTEMAVISLNSNKLRKMAEDG
                     DKDAIRMIKLVDEPAGFLSTIQIGITLAGFLASAFAADNFSDRIVDWLVQDLNFTAIS
                     PNALNTISVILITLVLSYFTLVFGELVPKRVAQKNAFAIAKRNTKFLVFFTKITKPIV
                     WLLSASTNGILKLLKKEDETDENEVTEEEIKFMVDVGEEKGAINNMEKTMIENIFEFN
                     NIFAKDIMTHRVDLVMIDINDPQEKALQTIEDGSYSRIPVFDDNIDNVIGILNVKDYL
                     IKLRKEGYVDIRSILRPAYFVPETIVCDALFRDMQASNSAIAVVVDEFGSLSGIVTME
                     DLLESIVGEIYDEYDISEEQEITRLENNLWRIAGSTEIEMIEEKLGIDIPDDVEFETM
                     GGLVFNALNTIPKDGSKLTVEAYGLSIRVEEITDHRVIWAFVRKKTGGPSGN"
     gene            15282..16514
                     /gene="ytvI"
                     /locus_tag="PLQ68_04115"
     CDS             15282..16514
                     /gene="ytvI"
                     /locus_tag="PLQ68_04115"
                     /inference="COORDINATES: protein motif:HMM:TIGR02872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YtvI"
                     /protein_id="HXK71870.1"
                     /translation="METDKKKSFIVNTLFIVIILALFYVLFKYAINLIMPFLIALIVS
                     IILKPVVKFFHKKLKLNRKIGAVIMVVLFYGIIGTVIILLSVQIGSARKDFLLKLPQA
                     FYQDIIPGFNRLFENINEWISKLDPDLLDPVQNAYDSLLAELGTFLATFSSKALSTVT
                     SYATSLPNTLLNVLITVIATYFITADFEILQKSLVRQFSAKNSVLYENIKIHLSGTVG
                     KYIKAYAIIFVISYAELALGLWIIGINNPFFVALLIALFDLLPVLGTGMILFPWTLIT
                     FLQGNFVRGIGLLILYIVIIVVRNIIEPKIIGEKVGMHPIVALLAMILGLRLFGIIGL
                     IGVPITVALVISLNRADAICIFKNEPCNINKNDDDKKKPKKPKKPLFKRKGPISPVIV
                     TENNNKNGSKESTDSIES"
     gene            complement(16552..17649)
                     /locus_tag="PLQ68_04120"
     CDS             complement(16552..17649)
                     /locus_tag="PLQ68_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006210591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HXK71871.1"
                     /translation="MKDKLRIGIIGTGGISQVHIKAYLSFDDVEIVAGADIISGKARQ
                     VLDKFGLTDAKDFDNHEDLCKMKGIDAVSICTYNSAHAVCTIDALSAGLHVLLEKPMC
                     VTMEEAYDIVRAEKKSGKILSIGFQPRYNPNCKKIKQIVSSGTLGKIYYVQTGGSRRR
                     GIPGSTFIDHDKAAFGAIGDLGCYSIDLAMNTIGHPKPLTVSAYTSDYFGKNPKYYKE
                     SERFSVDDFSVALIRLENDIVMDYRMAWAMHMDKCSDFVILGTDAGLKITTPDKPPLW
                     GSIIDGNFGHIYLMHDVCDEPVTTEISLPKEQVSQFTAKIRDFVDAVKENKPAPIPTK
                     EIIYNQAIIDAMVRSAKERREIKIEIPKDIL"
     gene            complement(17661..19400)
                     /locus_tag="PLQ68_04125"
     CDS             complement(17661..19400)
                     /locus_tag="PLQ68_04125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK71872.1"
                     /translation="MKHKYLELMKTAIDSYSYEYIEQLYRENQENGIKEHGISRITSV
                     LGILISKGMKQDYYDLWIKLMDMCCNQMHMIKVGNDFAVREIMSCIIETKNIVPKEKT
                     ELWLSLLKKIYYKENYNVYAKSVDDTAHNIAVYNMAGEYLRQINGMCDTEEYFDIQIP
                     CQLKRFDKNGMYMDPGCPMLYDLATRVQFSLLLFLGYDGKYSKQVDDNLKKGAMMSLK
                     MQSACYEIPYGGRSNQMVFNEAYQCACFEYEAARYKKEGNLELAGQFKRAAKLSYEAI
                     LRMVSTKPAKHVKNGFDWNSMFGCEWYAYYDVYMIALASFTYIAYIFSDDDIEEKPCP
                     AETGGYLVNTSEHFHKVFANTAGYSIQIDTLADKGYDATGLGRIHKSGVSPEIALSCP
                     FPRSPKYMIDDILMSKRPVQSEFNTLIRENPGFLSLCPAFKTEDNKIIALCDFYENLN
                     SVLNATKQQSDLIQFDITYTSPGFTGFTYIKEGYKLNEEGLSVCFTTDASETYKRMYL
                     VPIIKTNKENDSAVKIDNDTVTVDYRQHKYTVSAKDCTVEIKEEYANRNGVYSKVEFL
                     SKSDKIEINFSID"
     gene            complement(19403..20473)
                     /locus_tag="PLQ68_04130"
     CDS             complement(19403..20473)
                     /locus_tag="PLQ68_04130"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HXK71873.1"
                     /translation="MIDKQAYSLSNVQLKRYEKLDEEYMQMHTDPDNCIPKFIMPVRG
                     TFNPVWEEMLSDKEVMLKYHLEKHIPHMDVGDDCVLYARVDFGTCVVANAFGCDVFYP
                     VNNLPCAKDHIIKTKEQIYSLKTPDADCAPYKKVKEWTEFFVENLPDGYHMMMADIQG
                     PFNNAHLVRGTDIFYDMYDDIEAFDKLMEVVTDATIEYAKAQRQWADMKDGWQYDWSA
                     LWKGNARISNCSLHMIGRDLYIDHVMKHDIKLIEKMNGARMHYCGTAKNVIEEMAKIP
                     HITGIDYDSLLHDIEETMDNVPKDLTLLQSLSLSSDTAKKILSSKTWPFKKRNVIFSL
                     RGPMTIEEGKELYRRFRKVAEN"
     gene            20684..22366
                     /locus_tag="PLQ68_04135"
     CDS             20684..22366
                     /locus_tag="PLQ68_04135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK71874.1"
                     /translation="MKSKVLLTLILIFVLLFTACNNKELPENSSVVSTPFVDDLSVYV
                     LIGEIESSNEYLQNESNNIKVYGKNKDNLDNYTEIKFMYKDKEWISDEVRIFADKEME
                     REDIRQFNLSFYEDKDIISVIYYEKISSDEIKENILFFDQLSFTQLNPEDINISVQHQ
                     SSEEKVVIDETSYNLQTVIKAKIDPDIFDNKTVISKKNDIEVCALKKDSENNYSNIYV
                     KIGNLSKTIVTMDRKTYEFGGLYFNVLDVNLDGNDELVINFYEKTKDSELTQSVCFID
                     INTEKEVTLSDLRLENIVEVTYETDKRTITQEGKEYKVNTCHYTRKVELNKDNFHTVP
                     VKEIILISEEKGITLSVLSDPVSYITENGRPDIFALQYADKVELYNDWSMPKSAPISD
                     NEHTYEPFVDIGFIDLDDDELDEIIVSMFIGGGTESAEMELHIINGSDLNEIVTLTNQ
                     DMSLLLNEYIDSRVYIEDNQVKFDICINDDMSNIYAYSVNRNENATYFSTFGYASVVN
                     YILDSDTNLLIGEASVAIGITQFIGHFKASIILADGNVHLSNIQFVLNSDSN"
     gene            complement(22370..24757)
                     /locus_tag="PLQ68_04140"
     CDS             complement(22370..24757)
                     /locus_tag="PLQ68_04140"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HXK71875.1"
                     /translation="MIIHLNDDWHFYSSIESKEYETVRLPHSNVVMPYNCFDESVYQK
                     ISKYEKTIYVEDLWKNKAVLITFSAVAHQAKVYVNDMLVHTNSCGYTSFTVDISKYLE
                     FEKENKITVIADANETLNQPPFGNVIDYMTYGGIYREVYIDILEKTYISDVFITGKAR
                     RADGLSEFTADIEVATEAVDCEYEIDLSIYSVKDNTRIFEETYNTKESKIHVSEKIKA
                     ENWDLDNPNLYRFVAKLYHDEKLLDEFSDRFGFRDAIFKDDGFYLNGNKVKIIGLNRH
                     QSYPYTGYAMSKRVQINDADILKNELNVNAVRTSHYPQSKHFINRCDEIGLLVFTEIP
                     GWQHIGDEKWKEIAVDNVKNMIRQYRNHPSIILWGVRINESADDDDFYTRTNMVAKES
                     DPYRQTGGVRCIKKSSLLEDVYTYNDFTHSGKKHVIAKKKDVTDKDVPYLITEYNGHM
                     YPTKMFDDESHRVEHALRHTRVLNAVMGDDSVAGCFGWCMNDYNTHKDFGSGDRICYH
                     GVMDMFRNPKDAAYAYSSQSDKYPVMHIATSMSKGDYPMAMTKSIWAFTNCDYIKMSI
                     NGRHIKDFYPDKVRFKDLLHPPIEIDDFTGDILTKDLGFSEYSDKVIKKLLSYIFVNG
                     PENLPIRYMLMMATVLIKEKLTINKLTQIITSHVAGWGDNVKRYVFEGIKDGKTVATQ
                     IKEPLKQMKLLLLTDTSELKEDETYDTASVRIRAVDQNMNLLNYCNEPLRIVTEGPIR
                     LIGPDNISLKGGCAGVYIRTDKRCGKAVLRVIGNDISWETEFLVSCKKDRQIDLS"
     gene            complement(24770..25969)
                     /locus_tag="PLQ68_04145"
     CDS             complement(24770..25969)
                     /locus_tag="PLQ68_04145"
                     /inference="COORDINATES: protein motif:HMM:NF024241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXK71876.1"
                     /translation="MEHRKISLGLNIKYGVMQALYWMGFVATYNYAAYYLSDKGFNSS
                     DIGLILAFANIFAALLQPPIAAYADRALKSKLKSIIWILSLANIALTVLILVIKGHPY
                     LTAIFLMLIISFILTLHSFINSFAMEYANKGIQLNFTITRAVGSLGAALSSLGMGFIA
                     SRFGPEAIPLTYLVMFILIVAVVISFKPADKFGTLSDVEKPAYIPPTGALKFLWKYKK
                     FAGLLLGATLIFSSYNMVINFMINIVERLGYGTAEMGTALAIAGFLEVPGMALYIILV
                     KKRKCSTLLQLSSVFFTLKTLAVFLSGNIYHVYAAHITQLLSFAIFVPASVYYINNLM
                     PANDKIKGQAYMTTTITLGGVIGSLLGGTLIDALSVPSMLLIATIISALGTLFMIIFT
                     EKEKVTC"
     gene            26176..28233
                     /locus_tag="PLQ68_04150"
     CDS             26176..28233
                     /locus_tag="PLQ68_04150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK71877.1"
                     /translation="MNAKIQNYNNRPTIFIDNKPVPPVIYALTDWPGGRLSYEESTVF
                     SLKRFASAGVNLYQLDISFEDMWLKDGSLDISIALKQIQGIIDIKPDAFVFFRLHTNP
                     PKWWYADNESEWVRYANTEVYPEPDMNKIQSYLHYDQKPVPRPSFASQKWISDMSEVI
                     TRFLNELYKSPLSDHLTGIHIASGLYGENHYWAFVGNEPDMSEPMQRRFKKFKNNPNA
                     KVPGMDERYNPKDGIFFDPVKDADMIDYLTCQHEAVAESMIHFCKLVKDTWKRDIITG
                     VFYGYYVSLFGRAGTGGHLAEEMILNCPYIDYISAPQAYNKNLRNIGGAGVSRGLLES
                     ARLNNKLLLDEVDHPTHLGTLLGGMKVYPPYESLQILRRNALTSFISGMGFWYYDFGP
                     YNTSGWWNDPYYLNEVKELQRIFIKYYEKPYIKNADVLLVFDTKIQKHTALTENQDPI
                     TDKSCINTSYPMALRSGASIDTIYLSDFGKADLDKYKCIVFMNAFALTDIQKKEISQK
                     AYKKDRSVVWFFAPGYTDTKRNDISFCKEVSGFDLDSIAPMPKITVQCEGFSYFVDNS
                     SEESRLNKLFVPKDGNILGYINNTPALSHKVINGCDIYFSTIPFTTPEAFRYIFERSG
                     VHIYDNSNDAVLEGSNLLLIHAENEGDRTIRFKDSEVTVHFNAGETMLFDTTTKTVLR
                     RGE"
     gene            complement(28811..29746)
                     /locus_tag="PLQ68_04155"
     CDS             complement(28811..29746)
                     /locus_tag="PLQ68_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006188033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HXK71878.1"
                     /translation="MDNKFNMIQEILQKRADYNARLNLLPYDGSPEIKEINGKKYIYI
                     RKRIGGRLTSKYVDIYSDELYQLLLRTVKEARDLKKNIRQSDSELANLGYIEKDLSPK
                     VILNLDLARANMKSNIYDQAVLEGVSTTFPQTEDILENGIVNGVSVSDVQKILNLKHA
                     WEFILDKDVISYDSNYHILSHIAKLVNEGFYDDGGRTRNIPVKIGGTSYIPPIPLETM
                     ITENIISIIQTDEENIDIAIKLCLYCMKTQIFIDGNKRAAVIFANHYLISKGEGLIVI
                     PEKEVSAFKQILIAYYEGKNNKIHDFMKTKCWIKL"
     gene            29976..>31158
                     /locus_tag="PLQ68_04160"
     CDS             29976..>31158
                     /locus_tag="PLQ68_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HXK71879.1"
                     /translation="MLSKSSRFKEIQGPLLLIVMDGVGLSDNKEGNAVAAANAPTLKM
                     LMEKYPNTAIKAHGVAVGLPSDEDMGNSEVGHNALGSGQIYSQGAKLVFGAIEKKTIF
                     SGETWLKLKDNCVKNNSAMHFIGLFSDGNVHSNIAHLKAMLEQAKAEGVKKARVHILL
                     DGRDVGETSALEYVEPFEEYLNSLRSDNFDIAIASGGGRMNITMDRYEADWDMVRKGW
                     EIHVLGKGREFTSAKEAIETYRAETNVVDQFMPGFVITKDNKPVGTIEDNDSVIFYNF
                     RGDRAIEISKAFEDSNFDKFNRVRVPNVIYAGMLEYDGDLHIPKNYLVSPPFIKNTLG
                     ENLSKEGIKQYAISETQKFGHVTYFWNGNNSSKFKGETYVEVPSDNLPFEQRPWMQCA
                     QI"
BASE COUNT         9782 a         5910 c         5345 g        10121 t
ORIGIN      
        1 catgaccttg gcatatcagc tgatgctgat gaggtgttaa acatgaggga tggaaaatta
       61 ataatttgat atagatatat cttcactgtt accagcaaaa tgtgattaga acatgccttc
      121 ttcatatttt ttattgcata tacacttttc ctgttatata attcagcaca agataggtat
      181 cccatatggg aactgaagcg gaggtaatta atatgaagac acgtaaaatc gcaatagcgg
      241 gcataatggg tgcattgtgc atagtaatgg ctttgacgcc gatagggtat attcccattg
      301 gaccgacaaa agcgacaatc atacacattc cagtaatagt agcggcaatt ttagaaggac
      361 ctttggtagg agcaatagta ggcttgattt ttggcttatc cagtatttta aacgcattgc
      421 ttttccccac agtaacatca tatgtatttt taaatccatt agtatcagta gtgccaagaa
      481 tacttgtggg tcttgtagca tatctggtat tcgctttatt taagaagatg aaaaagcctc
      541 tttctgtgac tctgctcact ttgatgcttt ttgcaatagc atattatctt ctgaatcaat
      601 cgatatccaa tttccagtca ggcaatatct tttatgccat attcaatgta ttccttttac
      661 ttgtagtatt cggagcggaa atttacacaa tacttaatta tgagtcaaag aattttgcag
      721 ttgcattgtc tgctgctctt gcgacattga caaataccgt acttgtgctt actatgatag
      781 gagtattcta tggaggcgag tgggttgcca aactgggtgt tgagccttca atggcaagag
      841 actttttagt cggaattggt gttgcaaacg gaatacctga aacagtagcc gcagttataa
      901 tcggatcagt ggttgttgaa gcgcttatga agctggttta caagaaaaat aaaccgattg
      961 aagtaccaaa taatttaaaa accgagtaga tcatgtttgg taaccatacg atgaatcgta
     1021 tgttaactat atagactggc caggtatata cctggtcagt cgctttttaa tgattcaaga
     1081 ttcgacagaa aatccatccc ctcttttata ttccctttat actcattaag ttcaagtact
     1141 gtgtcaatct tatcccagac gacattgata aaatctctca ttactatgtt tgtataagaa
     1201 agccattgct cattatcttt aaggcttata acaaactgat tcatattatc acgatgttca
     1261 acttccctac ctaatggcga acccagtacc cattttgctg tcagctgtcc ttgaagtaca
     1321 aacagccctt tatccttttt catgtatctt tcataatagt caggatacgc attagctatg
     1381 aaatcttcac tgtcctgttt atgatatgct gaatcgtaat atgaatccag aactgtgtta
     1441 tctaaagatc cgctgtcgtt atttcttcta aaagcccatg acctttctgc gcagacatca
     1501 cttccccagc atattaatat cttacctctt actgaaccta aaggaatgtt atcaataaaa
     1561 tcaagatcgg taagatcagt ttcattgtat attagataat cagacaaatt tgtttcaata
     1621 actgctgcaa catcctccat ggaatcattc ttgaagtgtt ggaaatccaa tatgattaat
     1681 tctgtagtat tagtttccat aaattctaaa atatcatcta tgacagctgt aaaggtctga
     1741 cccttagcta tactgtgata tatgaccagc tcagcattct tttttttctc cacccttatg
     1801 tcaaaatacc taacaccatc tattagctgc ttgtgtatag gcaggctctg tgtcttagct
     1861 gcatatatca tccctatagt tccggcatca tggcttccag gcatgactat ttctttaatc
     1921 agagtatcat cttttatata actcatccat tctgaataga acggaatatc atgattcacc
     1981 agtatattct ggcatccgct catagaaagc agcatgactg atattattaa aactgcaaat
     2041 aacttcttca aaaaaccacc ccctgttacc gtatacgcct ttatttatat acatatatat
     2101 tatataccta atacaagtaa gctaaaaggt cataaatcct atcggctttc gctggtatag
     2161 ccttgtaagt tatgattgac gttcagccaa taacaatgac gcccgttcca atgcagtatc
     2221 gatatttgga caaaagtttt ccattccgac agcattatag aagccggctt tcttcatcat
     2281 ctccatcggt tgtgactgaa catgggaaaa tattagggta atgttatttt tcaagcagcg
     2341 gtcatgtact tttttcaaag aatcaagtgc tgtaatatcc atagcaggaa cactacgcat
     2401 acgtaaaatt atagagcata gtttcggatc ggtggtaatg ttcataaact gttgtgttgc
     2461 tgcaaaaaac aaaggcccgc ttatctcata taccatcgta tgcttgggaa caacctttaa
     2521 cgcaatagaa tccttgtcat tttcaatttc atcacgatca atgtcaatgt atttccagct
     2581 tttaatgcgt gtctcatctg ccatgcgttt catgaataaa aatgctgata taactatgcc
     2641 agcttcaata gcaataacca ggtcaaatac cacagtaaga atgaaggtga caagaagcac
     2701 cagaatatca cttttgggtg cggttttcac tatgctaaca acctcacgcc actctgacat
     2761 attgtatgca acgataaaca gaattgctgc aattgcaggc atgggaatca tggctgcata
     2821 aggcattaat accactaaaa ctaaaaccag aaccacagaa tgtaccattc cagcaatagg
     2881 agtacgtccc ccgtttttaa catttgcggc tgtacgggcg atagctcctg tagcaggaat
     2941 accaccgaac agagctgaag caacattgcc agcaccttgt gctacaagct ccatattgga
     3001 gcggtgacgg cttccaatca ttccatcaga taccacgcag gacaacagcg attccactcc
     3061 tgcaagaatg gctatggtaa tagcatctgg caaaagagaa ccaacagaag aaatagagat
     3121 tactggcaaa gaaaatgtag gtaactggtt agatatatca tataaagagc cgatggtgtt
     3181 gacatccata ccaaagacct tcactattat actgctcaca acaacagcta tgagagaacc
     3241 tggtatttta ccagaaattt tgggccagaa aattaaaatt gccagacata ttacaccaac
     3301 taaagcagcc tgccaattga gcgaccctgc actcttaaaa cattcgataa gcttatcaat
     3361 tgtctcaaca ggcttagagt taaaagtcag ccctaaaaaa tctttgaact ggcctataat
     3421 tatggtaacg gcaattcctg cagtaaaacc ggtggtgatg gttttaggta tatagcgaag
     3481 aagactccca aaccgcaaaa gccccataat taccaagatt attcctgcca tcaatgtagc
     3541 aagcactaat ccatccatac cattgcgtgc gacaataccc gctacaatag ttgcaaaagc
     3601 tgcggtaggt ccggtaatct gcactcgact tccacctaaa aaagaaacta caaagcctgc
     3661 cacaatagcg gtatatattc ccctttccgg gctgactcct gaagcaatag ccaatgcaat
     3721 agataacggc agtgctataa ttgccacaat gattccagca atcacatctt taaaaaattg
     3781 cttttttgta tactgcttca ttactgaaaa cagctttggt ttcagtttat tcactatgaa
     3841 ctcctttttt tatccctctt tagttttata tttatgaatg taaaatgtta acataatatc
     3901 taagcagttt ctaaaaatac tgatttgcct aacaaaactt ttattccaaa aaaccattta
     3961 tctgaactac gttttataac ttcaaatctt tcagtaaaaa ccacttatta agtgccccct
     4021 cagtacacat taggcactat tctagcacaa ttaaaacgtt ttgcaatgag attttttaat
     4081 tatttaaaag tagtgatatt ctattctttt ttttataacc ataatcactc tgagaaattt
     4141 ggctattaat acaagaagat acatagttaa tatgtcagca tagaaaaaga gactgctttc
     4201 gcaatctctt tagggggaaa tttataatct ctagggggta gtggttatgt atatatatta
     4261 atcaggtatt gtgaactcaa tattaatatt gtgtgaagta aatgtgaatt tactattttt
     4321 ttgcattttt cttttttttt agataaaaaa aagactgttt ggggtagata aacagtcttt
     4381 tgtctaaaaa agcgggggcc tttttaggtt tgtggtaagt acatagtaaa ccccaattgt
     4441 gatggaaagg tgaaaatgtt atgaacggac tgaaaattat aaaaggattg aatattttgt
     4501 aaaaaatatg tatcatatta atatcgaagt atgattggag acatttaatt gagcacagaa
     4561 gatagaataa ccattaaggg cggcaaaaaa cttgaaggaa atgtagttgt aggtggttca
     4621 aaaaatgcgg ctgttgcgat tatttgcgca tccgcaatga tcaaaggcaa agtaatacta
     4681 gaaaacatgc caaaaataac cgatatacag gttcttcttg acacgctttc acaaataggc
     4741 gcagtagtta actggacagg tgaaaaaact gttgaagtag atgctaccga tataaaatca
     4801 catatatgcg ataatactgc tttgagtact ttaagaggct cctactattt cgtcggagca
     4861 cttttaggaa gactcaagca ggcgaaagtt gtttttccag gcggttgtaa ttttgcccca
     4921 agaccaatag accagcatgt atatgcattt gaaacacttg gagctacagt agaaacaaca
     4981 ggcggatata tatcagcaaa agctcgtaag cttgtcggta ataatatata ttttgacaac
     5041 tattctgtcg gagctactat taactctatt cttacagcaa ctcttgctga aggaactacg
     5101 actatagaaa atgcaaacag agaacctcat gttgttgatg tagcaaactt tttaaatatt
     5161 atgggagcgg atataaaagg cgctggaaca aatacaatta gaataaaagg cgttaaagaa
     5221 ctcttgggag gtaaatccta ttctgtatgc cctgatatga ttgaagcagg aactcttatg
     5281 atagctgctg cagcaacaag aggaacaata acattgaata atgttactcc ggaacacatg
     5341 gattcacttt cagcaaaact aactgaaatg ggtgtcactg tagaaacaat ggatgactcc
     5401 attacagtta aagcaaatat aaaaagagta aaaagcacaa atattaaaac aatgccatat
     5461 ccgggattcc caacagatct gcatccgcag tttgcaacac ttttagcaat tgcagatgga
     5521 gaaagcagaa taacagaagg agtattccct acacgctttc agtatgtcga acagcttcat
     5581 aagcttggtg caaacataca gctaagcggc gaatctgcga ttatcaaagg agtaccaaaa
     5641 ctaattggag ctcctgtatc accaactgat cttcgtgcag cagcagctat ggtaatagca
     5701 ggacttattg ctgaaggcca gacaacaata tttaatcttg agtaccttga cagaggatat
     5761 gaagctttta tagaaaaatt aaaatcatta aaagcagtaa tagaaagagg gaagtaaaat
     5821 gaaacatgta gatatgataa gagaccttac caatgcaaat ggcatatcgg gttttgaaga
     5881 agaggtagtt ggagttataa aaaaatatgc agtatcggaa gacatttcag ttgatacaat
     5941 gaataacgtc tttttgacaa aaaagcagaa tactaatgac cgtcctgtaa ttatgataga
     6001 cggacattct gacgaagtag gttttatggt tcagttcata acagctaatg gtcttttaaa
     6061 attcatacct attggcggat ggcaggatgc aaacatagct gctcagaagg taagaatcag
     6121 aaacaaagac ggcaaataca taaaaggagt agtcgcatca gttcctcctc attttttatc
     6181 agctgctcaa aaagatgcac ctctgaaaat tgagaatatg ttaattgacg taggagctac
     6241 atcaagagat gaagtcataa atgattttgg tattgaagtt gctgcgccgg tagtacctga
     6301 tgttgagttc acaattaatg ataacggtat aatgtgtgca aaagcatttg acgacagaat
     6361 aggtgctgct gcagttatag ccacaatgga agagttagct gatgaaagcc ttgacataga
     6421 cttaatcgga actgtctcta cacaagaaga agtaggatta agaggagcag aagtaacagt
     6481 taaaactgtt aaacctgcta ttgcaattgt atatgaagga actcctgcag atgatacatt
     6541 taccaatcag tatgaaactc aggccgctct taaaaaggga cctcagttac gtcacagaga
     6601 tggaagtatg ataaccaatc caaaattcct tgctttcgta agacaggttg caaaagaaaa
     6661 caacatacca ttccaggatg cagtcagatc tggtggcggt acagatggag gaaagattca
     6721 tcttgtatct ggagccattc caacaatagt aatcggcatg ccagtaagat atgcacatac
     6781 ccactatgga tactgcgcat atgatgattt taaaaattct gttgaattag gaaaagcaat
     6841 attacgtaaa cttacaaagg atttgaagta ttaaaaaaga tgccggaacc ttcatgagtc
     6901 tttagttttg tggaaaagac gaagtagggg aaaatcataa atacatgaag tggcatctga
     6961 caagataata tcagtattca tatattaaca ccacagacaa atcagtatat tttgatacaa
     7021 tgctgatttt tttgtgtctt ttttgtctac tcaaaaacta ttttgaattc tgtattcact
     7081 ttaatataaa attcttaaat atcctatgca ggctaaacaa ccgtataaac actttacaaa
     7141 tagaacaaac ttagatatat tcatatcaag aggtaaatta tgagagcatc cggaatacta
     7201 cttcctattt tcagccttcc ttccccatat ggtattggaa ccattggtaa agaagcatat
     7261 gagtttgtag attttttggt gtctgccaat cagagatact ggcagatact tcctttaggg
     7321 cctactggtt acggtgattc accatatcag tccttctgca gctttgcctg caacagctac
     7381 cttattgatt tggatatttt agaggaaaaa caccttttgc aaaaagatga atacatcaat
     7441 gaatcttggt atgcacaaga ggataaggtt gattacggtt taatgtatca aaaaagaaat
     7501 gctgctctgg aaaaagcttt taaaagattt gatgtcaata atatcaagtt caaggcttac
     7561 tgccaggaaa attcctactg gatcaatgat tatgcaatat ttatggcctt aaaagaaaaa
     7621 tttaacggtg acaaatggca gttatggcct gatgatataa ggcttagata tcatcagtca
     7681 ctgatgaatt acggaacaat gctggcagac aggattctgt ttcataaatt cttgcagtat
     7741 gagttctttg cacagtgggc tgaacttaaa aagtatgcta acaataaggg tatttatata
     7801 atcggagata taccaattta tgtttcatca gacagttctg atgtatggtc aagacattat
     7861 ctgtttcaga ctgatgaaaa taggaatctt gcatcagtag caggagtacc tcctgatgcc
     7921 ttttctgcaa caggccagtt atggggaaat ccactttatg actggacttc tatggagaag
     7981 gatgatttct actggtggaa gcaaaggata ggtatggcct caaaccttta tgactatgtc
     8041 cgaattgacc acttcattgg cattgagagg tatttctcta tcccttctga tgctgaaact
     8101 gcagcttcgg gtacatatta tcagggtccg ggaatgaagc ttattgacgc tattaacttg
     8161 tcaaggaata atatgggaat aatcgctgaa gatctaggag ttcttactga caatgtcaga
     8221 aagcttttgg aaaagaccgg ttatccagga atgaaagtac tgcagttcgg attctctggt
     8281 gagcctgcca atgataatct tcctatgcat tacaaaaaga atttaatagc atatactgct
     8341 acacatgaca atgacacaac aaaaggatgg tacagaaaac tcgacagaaa gaacagagat
     8401 tttatgaaga aatacttaaa caggtcacgc attcatgtgc atgacctgat tttggaaacg
     8461 atgaaatctg tcgcagatac cgctattata cctatgcagg attggttatg tcagggttct
     8521 gaaagccgta taaattttcc ctctgtggcc tttggtaact ggacttacag gattagtaaa
     8581 aatatgctta cagataatct ggcaagtgaa atatctgcaa tgaccgttct ttgtgcaaga
     8641 taaatctttg taaaagataa agttacttgt tttcacactt aaccagatac tctttaatat
     8701 ccttagtaaa tgctttcaat acatcatctg cttcttcttt ggtgcaaccc atagcattta
     8761 tatatacttt gagtttaggt tctgtacctg aaggtctcaa agcataccac gatccgtctt
     8821 caaactcgta ttttaaagca tcctgctctg gcagtccttc ataaccattt atataatctt
     8881 tgcataccga gcatttaata tcattaatct ttacaagagg tttctttctc catctggaca
     8941 taacggcttt tttggtcttt gcccctttaa taccaggata ttctatagaa atctggtttt
     9001 ctgacatgta accgtatttt tcatatatat tattcagaac ctgatatagt gtcattcctt
     9061 gttttttgta atagcctgcc atttcgcaca tcatcattgc agcgcttact ccgtccttat
     9121 ccctggtaaa tgtttctatg cagtatccaa tgctttcttc ataaccaaat acatatctgt
     9181 attcatcagt tttttcaaac tcatttgcaa gtccgcatat atatttaaag ccagtcagag
     9241 tctcaaagaa atgcgtacca taatgttttg ctattcttgg aacaatagag ccgctgacta
     9301 tagactgtac tacagcatct tttttcgaat acataccttt tatctgtctc tgagataaca
     9361 tatattcagc tagtagtgcg cctgtctgat taccagtgca gaaaacatac tcttctttat
     9421 catcctttat catcattgca atcctgtcgc agtcaggatc tgtggcaatt atcatatctg
     9481 catctgttct tttaccatat tgcaacggta tatcaaacac ctttacattt tccggattag
     9541 gataaggtgt agtcggaaaa gtcccatcag gatttttatg ttcttctaca atagttacat
     9601 tgtgaaagcc tctttccttt agaatcctcg taacaggaac aagacctgta ccatacagcg
     9661 gcgtatatac tatgtttata tctttatcta tatcatcatt aagtgttaag gataatatct
     9721 cttttataaa catatcatcc atttccttac caagcattgt tatctgtcct gattttaaag
     9781 cttcgttaaa atcaacggtt tttacatcat tgaaaggatc tatttttgat atgttcgctg
     9841 ctatactgtc tgctatatcg tccagtatct gtgaaccttc ctcccagtac actttatacc
     9901 cattatactg tttggggtta tgacttgccg ttacattgat acctgttgca gcattaagtt
     9961 tccttattga atatgaaagc tgtggagtag gtctcaaact atcatatata tatactttta
    10021 tcccatttgc agcaagtata gatgcacact gtgctgcata ttcgtcagat tttatcctac
    10081 agtcaaaagc aataaccgca cctctttttt ccattccatg ttcaacaaca tacgaagcca
    10141 aagcctgggt agcctgacca ataacatatt tattcatacg attggttcct gcgcccatta
    10201 tgcctcttaa gcctgcagtt ccgaagtcca gagtcttata aaatctttct tctatttcct
    10261 tttcatttcc tttaagtgct tctagttcct taacagtttc caaatcagct ttttccagcc
    10321 attcgttata cttatccaga tatgtcatct ttgcaccctt cctgagccgt ctcctctcat
    10381 gagcttttcg acttcaggta ctgtaaatcg ggaaaaatct ccatatatgg tatgcttcag
    10441 acaacttgca gcaactgcaa attctaatgc atctttactg ttaccatatg tattaagccc
    10501 gtatatcagt cctgcagcaa acgaatctcc tccgcctact ctgtctacta tgtcagtaat
    10561 ctcatattta cggctcacat aaaaatcttt tctgtcatta agacatgctc cccatccgtt
    10621 tatatcagca tttctgcttt cacgtaatgt tattgctatt agttttatat tgggatatgt
    10681 ttttaatacc ttatcagaaa gttctttata tttgttttca tccagtgctc ctttagtaac
    10741 atccgcatca ttgtgtatat caagagcctt ctgaacatcc tcttcatttg ctatgcatac
    10801 atccacatat ttaactatgc tgggcattat ctctgcggct tttttcccgt atttccacag
    10861 attacctctg taattgaggt ccatggaaac agttaatccc gcctgcttag cagctgtcac
    10921 tgcttctaaa gtaactaatg cgctgttttg tgtaagtgca ggggttattc ctgtgacatg
    10981 gaaccatgaa gcattctcaa atatctttaa ccagtcaatg tctcctggtt tagttaggga
    11041 tatagcagaa tattccctgt cataaattac ctttgatggt ctttgtactg ctcctggttc
    11101 aagaaaatat attcccatac gtccgtttct gtaatgtatg tcatcaacat taactccaaa
    11161 ccctcttaac tgcctgacgc atgatttagc tatatcatta tcgggcaata ctgttacata
    11221 tgatgtatcc attccgaaat ttgcgcagga aacagcaaca ttggcttctc ctccgccaaa
    11281 agtcgcctct aatttatctg actgcataag tctttctcca tatggggact taagccttag
    11341 cattatctct ccaaaagtaa ctatcttacc cattcaatgc ctccttggct tcttttatgc
    11401 tgttgagtat ctgttttgca gtagcagtaa tcttatggta ctcacctgtc tttgcatatt
    11461 tggttatatt tccaccgaca cccactccac atgcacctgc tttaatccag tctttagcgt
    11521 tattctcatc aactccgccg ttaactacaa gattaagata aggcataggc cctttaactg
    11581 cttttatgaa ctgtgttcct aaaacttctc ctggaaatac cttaactatg tctactccag
    11641 cttcagtcgc catggaaatc tcccttggag tcatacatcc aggcattgct actactctgt
    11701 aacgatgaca catagttact gttggaatat ccagatgagg agcgattata tactgagctc
    11761 cggacagtat ggcggttctt gctgtctgtt cgtccattac tgttcctgct ccgagtataa
    11821 ggtctttttc atcgtatttt tttgacagct cctctattac gtgatgagct cctttgaccg
    11881 tgaatgttat ctcgataagt ttgactccgc cttctagaca ggcatctgtt atcctgtatg
    11941 cctcttcagc gctttgcgct ctgactacga ctatgagtcc gcttttcatt acattttcaa
    12001 ttacctgttc ttttctcata aaattacatc ctttcagaaa cggtttatat ttagccgtta
    12061 tatatctttt atattatatc ttaattttca aaaaattcac attccagcat tgcacataag
    12121 cagtgatata ccggaagatg cagttcctga actttgtatg tctctgattc cggaacttta
    12181 atacaaacat cagctagcga atttaaataa ccgccaagct ctcctgtcat cccgataacc
    12241 ggtattcgac atgctcttgc agtaatagca gcattattca cattccttgc attacctgat
    12301 gtgcttaggc acaatatagc tcctgcaagt tttctgtatg catatgcctg ctgggcataa
    12361 atcatgtcgt atgaggaatc attgccgata gctgttataa gtgaagtatg gctggaaaga
    12421 catatagccg gcaagccttc tgaaagattt tctctaattg attcatccag ccttggattg
    12481 ggttctacat gccttttttt cttgaatccc ttcataagct cccctacaat atgctctgca
    12541 tcagaagaag aacccccatt accgcatatg agaagaattt ttccgtattt atatgtttct
    12601 tccattattt caaatgccct cataatatcc gccttacatc ttactaatga tggatatgat
    12661 gttaacattt cttcaaaaac ctctaatgat tcccttttca tttcaaatgc cccttatctt
    12721 aattattcgc aacatattct gcagcaacag ccaatgcctc ataatcacct atcgattcac
    12781 caagtgaaga tggcagtatg tcgcaaacct gtgatgaaat agcaaggctt tctttgttta
    12841 atactctttt agcatgaggc caaagaagat tcctgtcttt ggtaaatatg cttcctattg
    12901 caatggcatc aggatttaat atgtctataa gcaccgcaag tgctcttcct agcatctcgc
    12961 ctgatcttgc atatattgcc ttggctaaat tatcaccttt tgcagctgcc tttgctacaa
    13021 tcagagcatc aagttgttca acatcctctt ctgtcgtgca gaatgaaacc ttattacctg
    13081 attgcatctc ttctttaacc atagcttttg ctaattgcga aataccgcct cctgagcaaa
    13141 agccttcaac agaacccatc ttgccgtatc ctacaggacc atattcagat aacctcacat
    13201 ggcctatctc gcctgccatg tcatttgttc cagtatacag tttcccgtta aggatgagtc
    13261 ccgctccaaa acctgtcccg aatgtgcaga atatgaaatt tctgtatccg aatccagctc
    13321 cccagaacca ttctgccaaa gcgcccgcat tggcatcatt ctgtaatact gcttttacat
    13381 taaaatgttt ttcaaaaata tcagttatcg ggatattgtc ccagcctatt aggttgggag
    13441 gtgagagtat tagccccttt tcactgttta aaggacctcc gcagcttata cctatgcagg
    13501 atatatcatt gctaattaaa aaattctttg ctagtatatc ctcaaggcca agaataagcc
    13561 tgtctattac agcagacgga ccattgcatg agtgagtctt gaaagtgact ttgtctacta
    13621 tatccacccg gattatatta ttgtccacac actttcccag tacagcagcg catttagtgc
    13681 cgccaatatc tatacctgct atatacatga cgaacctccg taatatgaat gttccatatt
    13741 aattatatca tttaacatat atattttgtg taaataaaca aatattctta tctgccctga
    13801 taatatccta agttggaaca tttttttata atttatatac aaattcatat atttttaatt
    13861 ttcaaactgt tttttgatat aatagtaagt gaatgaaatt tatatgatat catacacaag
    13921 aaatctgtta tttaatattt cattcaagga ggcaattttg aaagacccaa tcctgggccc
    13981 attaatttta cagatactgc ttatttccat taatgcattc ttcgcatcaa cggagatggc
    14041 ggtaatttca cttaattcaa ataaacttag aaaaatggca gaagacggtg acaaagatgc
    14101 cataaggatg ataaagctcg tggacgagcc agcaggattt ctatctacta ttcagatagg
    14161 aatcactctt gcaggctttt tagcttcagc ttttgctgca gataattttt ctgaccgaat
    14221 tgttgactgg ttagttcagg atttaaattt tacagcaatt tcaccaaatg ctctaaacac
    14281 aatatcagtt attctgataa cacttgtcct ttcctatttc acccttgtat tcggtgaact
    14341 ggttccaaaa agagtggcac aaaaaaatgc ttttgcaata gcaaaacgta acactaagtt
    14401 tcttgttttc tttacaaaga taaccaaacc tatagtatgg cttctttccg cttcaacaaa
    14461 tgggatatta aaacttctca agaaagaaga cgagacagac gaaaatgaag taactgaaga
    14521 agaaatcaag tttatggttg atgtaggtga ggaaaaaggt gcaataaata acatggaaaa
    14581 aaccatgata gagaatattt tcgaattcaa taatatcttc gctaaagaca ttatgacaca
    14641 cagggttgat cttgttatga ttgatattaa cgacccgcag gaaaaagcat tgcaaacaat
    14701 agaagacggt tcttattcaa gaatacctgt ttttgatgat aatattgata atgtaatagg
    14761 gatacttaat gtaaaggatt atcttattaa gttaaggaaa gaaggttatg tggatattcg
    14821 ttctatatta agacctgcgt actttgtacc ggaaaccata gtatgtgatg ctctttttcg
    14881 cgatatgcag gcaagcaatt ccgctatagc tgttgttgtt gatgagttcg gttcattaag
    14941 cggtatcgtt acaatggaag atttattaga atccatagta ggtgaaatat atgatgagta
    15001 tgacatctct gaagaacagg aaatcaccag actggagaac aatctttggc gtatagcagg
    15061 aagtacagaa atagaaatga ttgaagagaa acttggaata gatatccctg atgatgtcga
    15121 atttgagaca atgggcggac ttgtatttaa tgctttgaat actattccta aagatggttc
    15181 aaagctcaca gttgaagctt atgggttgtc aataagggtt gaagaaatta ctgatcacag
    15241 agtaatttgg gcatttgtaa gaaaaaagac agggggtccg agtggaaact gataaaaaga
    15301 agtcatttat agttaatact ttgtttatag ttattatact ggcattattc tatgttttgt
    15361 ttaagtatgc aatcaacctt ataatgcctt ttctgatagc gctgattgtc agcataattc
    15421 ttaaacctgt cgtaaaattc tttcataaga aacttaaact gaacaggaaa atcggtgcag
    15481 ttattatggt cgtactgttt tatggaataa taggtacagt aatcattctg ctatcagtcc
    15541 agattggatc tgcacgcaaa gatttccttc ttaagcttcc tcaggctttt tatcaggata
    15601 taatacctgg atttaacaga ctttttgaga atataaatga gtggatatca aaactggatc
    15661 ctgatctttt agaccctgta caaaacgcat acgacagtct tttagcggaa cttggcacat
    15721 tcttagcaac attttcctca aaagcattgt caacagtaac atcatatgcc acatctcttc
    15781 caaatactct tttgaatgtt ttaattacag ttattgcaac atactttata acagcagatt
    15841 ttgagatact tcaaaaatca ttagtaagac agttttcagc aaagaattcc gttctgtatg
    15901 agaatataaa aatacacctt tcaggaacag taggaaaata tattaaagct tatgcgataa
    15961 tatttgtaat atcatatgct gaactcgcat taggattgtg gattatagga ataaataatc
    16021 cgttttttgt tgctttgcta atagctttat tcgaccttct gccggttctt ggtacaggta
    16081 tgattctttt cccatggacg cttattactt tcctgcaagg caactttgtg agaggtattg
    16141 gtcttttaat tctttatata gtaataatcg tggtaagaaa cattatcgaa ccgaagataa
    16201 taggtgaaaa agtcggtatg catccgatag ttgctcttct tgctatgata cttggattac
    16261 gcttgtttgg aattatagga ttaataggcg ttccaataac agtcgcattg gtcattagcc
    16321 ttaacagggc tgatgccata tgcatattca agaacgagcc ttgtaatata aataaaaatg
    16381 atgatgataa gaaaaaacca aagaaaccga aaaaacctct ctttaaaaga aaaggtccga
    16441 taagcccggt tatagttact gaaaacaata acaagaatgg ttccaaagaa tctacagatt
    16501 ctattgaatc ttaattaaat aactctaata acattttaat taaagaacat cctacaggat
    16561 gtcttttggt atctctatct ttatctctct tctttctttt gctgagcgta ccatggcatc
    16621 tattattgcc tggttgtata ttatttcttt tgtaggaata ggagcaggct tgttttcctt
    16681 tactgcatct acaaagtccc ttatcttagc tgtaaactgg ctaacctgtt cttttggcag
    16741 tgatatctct gttgtgacag gttcatcaca tacatcatgc ataagataaa tatgtccaaa
    16801 atttccgtca attattgatc cccagagagg aggtttatca ggtgtcgtta tttttaatcc
    16861 tgcatctgtg cctagtatta cgaaatcaga gcacttgtcc atatgcatag cccaagccat
    16921 gcggtaatcc attactatgt cattttccag ccttattaaa gccactgaaa agtcatctac
    16981 actgaatctt tcgctttctt tgtaatattt aggattcttc ccaaaatagt cagatgtata
    17041 tgccgatact gtaagaggtt ttggatgacc gatagtattc atagccaaat ctattgagta
    17101 acagcctaaa tcacctattg caccaaaagc tgctttgtca tgatctataa atgtacttcc
    17161 aggtatacct cgtcttctgg aacctccagt ttgaacataa tagatttttc ccagtgtgcc
    17221 tgatgataca atctgtttta tctttttaca gtttggatta tatcttggct ggaaccctat
    17281 ggataatatc ttcccgctct tcttttcagc tcttacaata tcataagcct cttccatggt
    17341 aacacacata ggtttttcca aaagcacatg tagtcctgca cttaatgcat ctatagtaca
    17401 tactgcatga gctgagttat atgtacatat tgatactgcg tcaattccct tcattttgca
    17461 cagatcctca tgattatcaa aatccttagc atcagtaagg ccgaatttgt ccagaacctg
    17521 tctggcctta ccagagatta tatctgcacc tgctactatt tccacatcat caaagctcag
    17581 gtatgcttta atatgtacct gagatattcc gcctgtacct ataatcccga ttcttaattt
    17641 atctttcata taatcctcca ttaatctata gaaaaattaa tttcaatctt gtcgctttta
    17701 cttaaaaatt ctactttact gtatacaccg tttctgtttg catactcttc tttaatttca
    17761 acagtacagt cttttgccga tacagtatac ttatgctgtc tgtaatcaac agttacagta
    17821 tcattatcaa tctttacagc agaatcattt tctttgtttg ttttaatgat tggaacaaga
    17881 tacatcctct tgtatgtttc actagcatca gtcgtgaaac atacactaag cccttcttca
    17941 tttaatttat atccctcttt tatgtatgtg aaacctgtga aaccaggtga tgtgtaagtt
    18001 atatcaaact gaatcaggtc actttgttgt tttgttgcat taagtacgga attcaaattt
    18061 tcataaaaat cgcataaggc gattatcttg ttatcctctg tcttaaatgc aggacacagt
    18121 gataagaaac caggattttc acgtatcaat gtattaaatt ctgattgtac tggtcttttg
    18181 ctcatcagta tgtcatcaat catatatttc ggcgatctcg gaaatggaca tgataatgct
    18241 atttcaggcg atactccgct tttatgtatc cttccaagac ctgttgcgtc atatcctttg
    18301 tcagctaatg tgtcaatctg aatagaataa cctgctgtat ttgcaaaaac cttatgaaaa
    18361 tgctcacttg tatttacaag ataacctcct gtttcagctg gacaaggttt ttcttcaata
    18421 tcatcatctg aaaaaatgta tgcaatatat gtaaatgagg caagagctat catatataca
    18481 tcatagtatg cataccactc acacccaaac atggaattcc agtcaaaccc gttcttaaca
    18541 tgctttgcag gttttgtact taccattctt aagattgctt cataagataa tttcgcagct
    18601 cttttaaact gtcctgctag ttccaggttt ccttcttttt tgtatctagc agcttcatat
    18661 tcaaaacagg cacactgata agcctcattg aagaccattt ggtttgatct tcctccatat
    18721 ggaatctcat aacaagccga ttgcattttt aaactcatca tggcaccctt tttaaggttg
    18781 tcatccacct gctttgaata ttttccatca tatcccaaaa aaagcagcag tgaaaactgc
    18841 actcttgttg caagatcata tagcatagga catcccgggt ccatatacat gccattttta
    18901 tcaaacctct taagctgaca aggaatctga atatcgaagt attcttcagt atcacacatt
    18961 ccattaatct gtcttagata ttcacctgcc atgttgtata cagctatgtt atgtgctgta
    19021 tcgtcaactg atttagcata tacattgtag ttttctttgt agtatatttt ctttagaagt
    19081 gaaagccata attctgtctt ttccttaggt acaatatttt tagtttcaat aatacaggac
    19141 atgatttctc ttactgcaaa atcatttcca acttttatca tatgcatctg gttacagcac
    19201 atatccataa gtttaatcca aaggtcataa taatcctgct tcatgccttt ggatatgagt
    19261 attccaagaa ctgatgttat cctggatatc ccgtgctctt taattccgtt ttcctgattc
    19321 tctctataca gctgctctat gtattcataa ctgtaagagt caatagctgt cttcatcagt
    19381 tctaaatact tatgtttcat aattaattct ccgctacttt tctgaatctt ctgtataatt
    19441 cttttccctc ttcaatagtc atgggacctc ttaatgagaa tatgacattt cttttcttaa
    19501 atggccatgt cttggaagaa agaatctttt ttgccgtgtc agaggataat gataatgact
    19561 gtaaaagcgt caagtctttt ggtacattgt ccatggtctc ttcgatatca tgcagtaaac
    19621 tgtcataatc aatacctgta atatgaggta tttttgccat ttcttctatt acattctttg
    19681 ctgtaccgca gtaatgcatc ctcgctccat tcatcttttc aataagcttt atatcatgtt
    19741 tcattacatg gtctatgtat aaatctcttc caatcatatg caaagagcag ttggatatcc
    19801 tggcatttcc cttccacaaa gctgaccagt catattgcca tccgtctttc atatcagccc
    19861 actgtctctg ggcttttgcg tattcaatag tggcatcagt aacaacttcc ataagtttat
    19921 caaatgcttc tatatcatca tacatatcat aaaatatgtc tgttcccctt accagatgcg
    19981 cattattaaa tggtccttgg atatctgcca tcatcatatg ataaccgtca ggcaggttct
    20041 ccacaaagaa ctccgtccat tccttgactt ttttgtaagg agcacagtct gcatcaggag
    20101 tttttagaga gtatatctgc tcttttgtct taataatatg gtcttttgca caaggcaggt
    20161 tattaaccgg gtagaagaca tcacagccga atgcattggc aaccacacag gttccgaaat
    20221 ccactcttgc atacaaaaca caatcatctc caacatccat gtggggaata tgcttttcaa
    20281 ggtgatattt gagcattacc tctttatcag aaagcatttc ttcccataca ggattaaatg
    20341 ttcctcttac cggcattatg aattttggta tgcaattatc agggtctgta tgcatctgca
    20401 tgtattcttc atcaagtttt tcatatcttt ttaattgaac attactgagc gaataagctt
    20461 gtttatctat catgacgact ccttttcgaa ggttagatag attatatcat catacatata
    20521 tccaaaacat attaacttgc tattacagaa aagttactgc agaacaaaat gcgtgttttt
    20581 acaaaacttc gtataatcca gcatggtatg ttgtattata cgagattata tattaaaata
    20641 tatgatggaa catgaatgca caacaactta caagaagttc tctatgaaat ccaaagtatt
    20701 attgacactt attctgatat ttgtattact tttcacagcc tgcaataata aggagctgcc
    20761 agaaaacagc agtgtagtaa gcactccttt tgttgatgat ctatcagtat atgtcttaat
    20821 aggagaaata gagtcttcca atgagtattt acagaatgaa tcgaataata taaaggtata
    20881 tggaaaaaac aaagacaatc tcgacaatta tacagaaatc aagtttatgt ataaggacaa
    20941 agaatggatt tctgatgagg taagaatatt tgctgataaa gaaatggaaa gagaagacat
    21001 aagacaattc aatctgtcat tttatgagga taaggatata atttctgtta tttattacga
    21061 gaagatatca tcagatgaaa taaaagaaaa tatactgttc tttgaccaac taagctttac
    21121 acagcttaat ccggaagata ttaatatttc tgtccagcat cagtcatcag aagagaaggt
    21181 cgttattgat gagacatcat ataacttaca gacagttatt aaagccaaga tagatcctga
    21241 tatatttgac aataaaaccg ttataagtaa aaagaatgac attgaagtat gtgcgcttaa
    21301 aaaggatagc gaaaacaact acagtaatat atatgtaaaa atcggcaact taagcaaaac
    21361 catagtaaca atggatagaa aaacctatga attcggtgga ctgtatttca atgttctgga
    21421 tgtaaatctg gatgggaatg atgaacttgt tataaacttc tatgaaaaaa ccaaagattc
    21481 tgagttaact caaagtgtgt gctttataga tattaatact gaaaaagagg ttactctttc
    21541 tgacttaaga ttggaaaaca tagtagaagt aacatatgaa accgataaac ggacaataac
    21601 tcaagaaggt aaggagtata aagtaaatac ctgtcattat acaaggaaag tggagcttaa
    21661 taaggacaat ttccatacag taccagtaaa agagataatc cttatatccg aagaaaaagg
    21721 gattacctta tcagttttat ctgaccctgt ttcatatatt acagaaaacg gcagaccgga
    21781 tatattcgca ttacaatatg ccgacaaggt tgaactgtat aatgactggt ctatgccaaa
    21841 atccgcccca ataagcgata atgagcatac ttatgaacct tttgttgata tagggtttat
    21901 agatctggat gatgatgaat tagatgaaat tatagtctca atgtttattg gagggggaac
    21961 ggaatcggct gaaatggaac ttcatattat caatggttcc gacttaaatg aaatagttac
    22021 tcttacaaac caggatatga gtttgctatt aaatgaatat attgattcaa gagtatatat
    22081 agaagacaac caggttaaat tcgatatttg tatcaatgat gacatgagca atatatacgc
    22141 atatagtgta aatcgcaacg agaatgctac atacttcagt acctttggtt atgcttcagt
    22201 cgttaactat atcctggatt cagatactaa tcttttgata ggtgaagcat cagttgcaat
    22261 aggaataaca cagtttatcg gtcattttaa agcgagtata atattagctg acggtaatgt
    22321 tcatcttagt aatattcagt ttgttttaaa cagtgatagc aattaactat taagataaat
    22381 caatctgccg atctttcttg caacttacca gaaattcagt ctcccatgat atatcattcc
    22441 ctattaccct taagaccgct ttgccgcacc ttttatctgt tcttatatat actcctgcac
    22501 atccgccttt taatgatatg ttatcaggac ctataagtct tatcggtcct tctgtaacta
    22561 tccttaaagg ttcattgcag tagtttaaaa gattcatatt ctggtctacg gctcttatcc
    22621 ttacagatgc tgtatcatat gtctcatctt cttttaattc tgatgtatcg gttaacagta
    22681 ataatttcat ctgctttaat ggttctttta tctgtgttgc tactgttttt ccgtctttga
    22741 taccttcaaa aacataccgt ttgacattgt ctccccaacc tgcaacatgt gatgttataa
    22801 tttgcgtcag tttattaatt gtaagctttt cttttattag tactgttgcc atcataagca
    22861 tgtatcttat tggcagattc tccggcccgt ttacaaatat atatgataag agtttcttaa
    22921 tgactttatc ggaatattcg ctaaatccta aatcctttgt aagtatatcc cctgtgaaat
    22981 catctatttc tatcggagga tgcaataagt ctttaaatct taccttgtca ggataaaagt
    23041 cttttatatg ccttccgttt atactcatct taatgtagtc acagttagta aatgcccata
    23101 tgctttttgt catggccatt ggataatcgc cttttgacat agaagtcgct atatgcatta
    23161 ccgggtattt atctgactgt gatgagtacg catatgcagc atctttcgga tttctgaaca
    23221 tatccattac tccgtgataa catatcctgt caccgcttcc aaaatcctta tgcgtattgt
    23281 agtcattcat gcaccacccg aaacacccag ctacactatc atcccccatg acagcattta
    23341 aaaccctggt atgacgaaga gcatgctcaa ctctgtgtga ttcatcatca aacatcttag
    23401 ttggatacat atgcccgtta tactctgtta tgaggtatgg gacatcctta tccgttacat
    23461 ccttcttttt agctattacg tgttttttac cgctgtgggt aaaatcattg tatgtataaa
    23521 catcctctaa aagactgctt ttctttatac atcttacccc gccggtctgc ctgtacggat
    23581 ctgattcttt tgcaaccata tttgtcctgg tataaaagtc gtcatcatct gctgattcat
    23641 ttatcctgac accccatagt attatgcttg gatgattcct gtattgtctt atcatgtttt
    23701 ttacgttatc aacagctatt tctttccatt tctcatcccc tatatgctgc catcctggta
    23761 tctcagtgaa cactagaagc cctatttcat cacatctgtt aataaagtgt ttgctctgag
    23821 gataatgtga tgttctgact gcgtttacat tcaattcatt tttaagtata tccgcatcat
    23881 taatctgtac tcttttgctc attgcatacc ctgtatatgg atatgactga tgcctgttaa
    23941 gccctattat cttaactttg ttaccattaa gataaaatcc atcatcttta aatatggcat
    24001 ctctaaatcc gaacctgtca ctaaactcat ccagaagctt ttcatcatgg tacagctttg
    24061 caacgaagcg gtaaaggtta ggattatcta aatcccagtt ttcggctttt atcttttctg
    24121 acacatgaat tttagattct tttgtgttat atgtttcttc aaatatcctt gtattatcct
    24181 taacagaata aattgaaaga tcgatttcat attcgcaatc cactgcttca gtagcaactt
    24241 ctatatctgc tgtaaactcg gataagccgt cagctcgtct tgctttcccg gtaatgaata
    24301 cgtcagatat atatgttttt tccaatatat ctatatatac ttccctgtat atacccccat
    24361 aagtcatata gtctatgaca ttaccaaaag gcggctggtt caatgtctca ttagcatcag
    24421 ctataactgt tattttgttt tccttttcaa attcaagata ttttgatata tctaccgtaa
    24481 atgatgtata tccgcaacta tttgtatgta caagcatgtc atttacatat accttcgcct
    24541 gatgagcaac tgcagagaaa gtaatcagga ccgctttgtt tttccataaa tcctctacat
    24601 atatggtctt ttcatacttt gatattttct gataaaccga ttcatcaaaa cagttatagg
    24661 gcataacaac attactgtgc ggcagtctga ctgtctcata ttcttttgat tctattgatg
    24721 aatagaagtg ccagtcgtca tttaaatgaa ttatcatata aagcctcctc tagcaggtta
    24781 ctttttcctt ttcagtgaat ataatcataa aaagagtgcc taaagctgat attatagttg
    24841 ctatcaataa cattgaaggt actgataatg cgtctattag agtacctcct aacaagcttc
    24901 cgataacacc gcctaaagtt attgtagtag tcatgtatgc ctgccctttg attttatcat
    24961 tagcaggcat cagattattt atatagtaaa cagatgccgg gacaaatata gcaaaggaca
    25021 aaagctgagt tatatgcgct gcatatacat gatagatatt tcctgataaa aaaactgcta
    25081 atgttttcaa tgtaaaaaat actgaagaga gttgtaaaag ggtgctgcat tttcttttct
    25141 ttacaaggat gatgtataag gccatgccag gtacttccaa aaatcccgct atagccaaag
    25201 ccgtccccat ttctgctgta ccatacccta atctttcaac aatattaatc atgaaattta
    25261 taaccatatt atagcttgag aaaattaagg tcgcgcctaa taacaaccct gcgaacttct
    25321 tgtacttcca caaaaacttt aaagcgcctg taggcggtat atatgcaggt ttttccacat
    25381 cagacaaggt gccgaactta tccgctggct taaaggatat cacaacagca actatgagga
    25441 taaacataac aagatatgtt aaaggaatcg cttcaggtcc gaatcttgaa gctataaatc
    25501 ccatacctaa agaagaaagt gccgcaccta aggaaccgac tgctcttgta atggtgaaat
    25561 tgagctgtat ccctttattg gcatattcca tggcaaagga attaataaat gaatgtaatg
    25621 taagtatgaa tgatattatt agcattaaaa atattgctgt aagatatgga tgccctttta
    25681 taaccagaat cagaactgtt aatgcgatgt ttgccaaaga taatatccat attatgctct
    25741 ttaattttga ttttaatgcc ctgtcagcat atgctgctat cggaggctgc aaaagagctg
    25801 caaaaatgtt tgcaaatgca agtataagcc ctatatccga actgttaaac cccttgtccg
    25861 aaagataata tgcagcataa ttgtatgtcg ctacaaaacc catccagtaa agagcctgca
    25921 ttactccata cttaatattc aaacctaatg atatcttcct atgttccata aattcaccta
    25981 tacagaaatt gtaacagaaa aggaggaaga attcattaaa agactttaca tatatggttt
    26041 tactgctctt tttctttacc ctgctattta tcgttttcca catgtctgtt ctaagaacat
    26101 taatagaagt aagtttaaaa aattgctgta cttaatatga tatagtatac atatatttat
    26161 taaggagttt ttattatgaa cgctaaaatt caaaattaca ataacagacc aaccatattt
    26221 atagataaca agcctgtacc gccagtcata tatgctctga ctgactggcc gggcggcaga
    26281 ttaagttacg aggaaagtac tgtattttcc ttaaagagat ttgcgtctgc tggtgttaat
    26341 ctgtatcagc tggatatatc ttttgaagat atgtggctaa aagatggttc tttggatata
    26401 tccatagcat taaagcagat acagggaatt atagatatta aacctgatgc ttttgtattc
    26461 ttcagactgc atacaaatcc tccaaaatgg tggtacgcag ataatgaaag cgaatgggta
    26521 agatatgcaa atactgaagt ttatcccgaa ccggatatga ataagataca aagctatctt
    26581 cattatgacc agaaaccagt accaagaccc agcttcgctt ctcaaaaatg gataagcgat
    26641 atgagtgagg ttataaccag gtttttgaat gagctgtata aatccccatt gtccgatcat
    26701 ctgaccggga tacatatagc gagcggacta tatggagaga atcattactg ggcttttgtc
    26761 gggaatgaac ctgacatgtc agaacctatg cagagaagat ttaaaaaatt caaaaacaat
    26821 ccgaatgcaa aagtaccggg aatggatgaa agatacaacc ctaaagacgg aatattcttt
    26881 gacccggtaa aagatgctga catgattgat tatcttacct gtcagcatga agcggttgca
    26941 gaatcaatga ttcatttttg caaacttgtt aaagacacat ggaaaagaga tatcataaca
    27001 ggtgttttct acgggtacta tgtatcttta tttggaagag caggaacagg agggcattta
    27061 gctgaagaga tgatactgaa ttgcccatat atagactata tctcagcacc tcaggcatat
    27121 aacaagaatc taagaaatat aggtggagca ggagtcagca gaggattact ggaatcagca
    27181 agattgaata acaagctgct gcttgatgaa gtagaccatc cgacacattt gggcaccttg
    27241 cttggtggga tgaaggtata tccgccttac gaatctcttc agatattaag aagaaacgct
    27301 ctgacttcat tcatatccgg aatgggattc tggtactatg atttcggacc atataacaca
    27361 tcaggctggt ggaatgaccc gtattatcta aatgaggtca aagagctgca gaggatattc
    27421 atcaagtact atgaaaagcc atatataaag aatgctgatg tgcttttggt atttgacaca
    27481 aaaatacaaa aacatactgc acttacagaa aaccaggacc ccattacaga taaatcatgc
    27541 attaatacat catatcctat ggctttaaga tccggcgcct ctatcgacac tatatatcta
    27601 agcgattttg gaaaagctga cctggataaa tacaaatgca tagtattcat gaatgcattc
    27661 gcacttactg atatacagaa aaaagaaata tcccaaaaag cttacaaaaa agaccggtca
    27721 gtcgtgtggt tctttgctcc tggttatact gataccaaaa gaaatgatat atcattctgt
    27781 aaggaagtat caggttttga tttggattct attgcaccaa tgcctaagat aactgtacag
    27841 tgtgaaggtt tttcctattt cgttgacaac agcagtgaag aaagcagatt aaataaactg
    27901 tttgttccta aagatggaaa catattagga tacataaata atactccggc attatcccat
    27961 aaagtaataa acggctgtga tatatatttt tccacaattc cttttactac ccctgaagca
    28021 ttcagataca tatttgaaag aagcggagtc catatttatg ataacagcaa tgatgctgtt
    28081 cttgaaggct caaatcttct attaatacat gcagaaaatg aaggagacag gactataagg
    28141 ttcaaagatt ctgaagtcac cgtgcatttc aacgctggag agaccatgct gttcgacaca
    28201 acgacaaaga ctgtgttaag aagaggggaa taagatgtta aggtataagg cggccatctc
    28261 ttaaacttag cgttttatca gcaacagaag caatatccaa gtcatgcgtt acaacgatta
    28321 cgcagaattt attattgtgt gcaaggtctt ttaattatga aactgtttgg tttacatccc
    28381 aataatacga gtcatgcgaa catgcagcat atgcaatact tgctaaccac aaaataatag
    28441 caatggttat tattcatata ccaattcttt gtaacttttt catgaactaa tacctccgaa
    28501 ttatgtattt gtgtaatatg ttagtataat atcaataaat attagattgt tcaagttaat
    28561 tactagatat gatagagtca atttactata gaattaaata atgactatca tcacaaaatt
    28621 ttctttttta gaagatacaa attattagaa ttgcaatatt acatcctata ttttccttga
    28681 acaacatatg tactaggcct ctcttctgtg cattagaaga agcatcttca cacagtaatg
    28741 gattcctcac atatcttttg ataatattac attctttatg cataatttta aatatatatt
    28801 actttttata ctatagtttt atccagcatt ttgtcttcat aaaatcatgt attttattat
    28861 tctttccttc ataatatgca atcaatattt gtttgaatgc tgatacttct ttctcaggaa
    28921 ttactattaa cccttcacct tttgatatta aataatgatt agcgaatata actgcagctc
    28981 ttttgttacc atctataaaa atctgtgttt tcatacagta caaacataat ttaattgcaa
    29041 tatcaatatt ctcttcatca gtttgaatga tgctaattat attctcagtt atcattgtct
    29101 ctagcggtat aggaggaata tatgatgtgc cgcctatttt aacaggtata tttcttgtcc
    29161 ttccgccatc atcataaaat ccttcattaa ccagttttgc gatatgactt agaatatgat
    29221 aatttgaatc ataactgata acatctttat ctaatatgaa ttcccatgca tgttttaaat
    29281 tcaatatctt ttgtacatcg gaaacactta ctccatttac aataccattt tcaagaatat
    29341 cttctgtttg aggaaaagtt gttgatactc cttctagaac agcttgatcg tatatatttg
    29401 acttcatatt tgctcttgct aaatctaaat ttaatatcac ttttggagat aaatcctttt
    29461 caatgtatcc aagattggct aattcactgt cgctttgcct aatatttttt ttcagatctc
    29521 tcgcttcttt gacagttctc aataatagct gatagagttc atcagaatat atatcaacat
    29581 attttgaagt taatctgccg ccaatgcgct ttcgaatata tatatatttt ttcccattta
    29641 tttcttttat ttccggtgag ccatcatatg gcagtaaatt caatctggca ttgtagtccg
    29701 ctcttttctg tagtatttcc tgtatcatat taaacttatt atccatttac tttctcctta
    29761 gggaactatt gcacttaaat tatatataat tgttccctat aaagcaatac atatagggaa
    29821 ctttttagtt tattttatgc acatttgttc cctatatgct ctcttagaaa tggactatgt
    29881 attttaacct acttaatgta taaattgatt ggcatatgac ggtatgatat aatttgttgg
    29941 aagtacatac tagtattacg gaaggaagaa ataatatgct aagtaaaagt tcaagattca
    30001 aagaaataca aggaccgctt ctgcttatag tcatggatgg agtgggatta tcagataata
    30061 aagagggaaa tgctgtagca gctgcaaatg ctcctacact taaaatgctg atggaaaaat
    30121 atcctaacac agctataaaa gctcatggag tagcagttgg attaccaagt gatgaagata
    30181 tgggcaactc cgaagtaggt cataatgcat taggctcagg acagatttac agccagggcg
    30241 ctaagcttgt gtttggggca atagaaaaga aaaccatttt cagtggagaa acatggctta
    30301 aacttaaaga taactgtgta aaaaataact cggctatgca ttttataggg ttattttctg
    30361 acggtaatgt gcattcaaat atagctcacc ttaaggcaat gcttgaacaa gcaaaagctg
    30421 aaggcgttaa aaaagcacgt gttcatattc tccttgacgg aagagatgta ggggaaacat
    30481 cagctttgga atatgttgaa ccttttgaag agtatcttaa cagcttacgt tcagacaatt
    30541 tcgatatagc gatcgccagc ggtggcggca ggatgaatat cacaatggac agatatgaag
    30601 ctgactggga catggttaga aaaggctggg agattcatgt acttggaaaa ggccgtgaat
    30661 ttacttctgc aaaagaagct atagaaacat acagggctga aacaaatgta gtagaccaat
    30721 tcatgccggg ttttgtaata acaaaagaca ataagccggt aggaaccata gaggataatg
    30781 acagtgtgat cttctacaac ttccgcggag acagagctat agagataagc aaagcatttg
    30841 aagacagcaa tttcgataaa ttcaacagag taagagtacc aaatgtcata tatgcaggaa
    30901 tgctagaata tgacggagac cttcatatac cgaagaatta tttagtaagc cctcctttta
    30961 ttaagaacac attaggagag aatctttcta aagaaggaat aaagcagtat gcgatatccg
    31021 agactcagaa attcggacat gtaacatatt tctggaatgg taataattct tccaaattca
    31081 aaggagagac atatgttgaa gtaccttcag ataatcttcc tttcgaacag cgcccatgga
    31141 tgcagtgcgc acagataa
//
LOCUS       DAOSXM010000018        29932 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2111
            NODE_985_length_29932_cov_12.367272, whole genome shotgun sequence.
ACCESSION   DAOSXM010000018 DAOSXM010000000
VERSION     DAOSXM010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864178
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 29932)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29932)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:24:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,261
            CDSs (total)                      :: 2,229
            Genes (coding)                    :: 2,218
            CDSs (with protein)               :: 2,218
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 3 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29932
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_985_length_29932_cov_12.367272"
                     /isolate="AMR_MDS_2111"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            359..1390
                     /locus_tag="PLQ68_04165"
     CDS             359..1390
                     /locus_tag="PLQ68_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009595428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 130 protein"
                     /protein_id="HXK71880.1"
                     /translation="MLDEATRKIVHACPKEALDESLFSTSSIITRYPETILSRHDVPY
                     PAALTFNAGVVKEGDRYIMIFRNDYGSYEDQRIDGTNLGLAFSKDGIKWDVEPEPCFA
                     MADDEVLRVYDPRITVMDDRYIICFAVDTKHGVCGGIAETKDLKKFDVVSISVPENRN
                     MVLFPEKINGNYVRLERPMPVYGRDGMKFDIFLSESPDLVYWGKSRLVMGVEHVPFAN
                     EKIGPGAPPIKTDKGWVMLFHGVDFDPARGKNGWEETWKKRYTIGVALLDLEDPSKVI
                     GMYKRPLIIPEGYYELIEGYRTNAIFPCGALVDDNNQVRIYYGAGDAIIRMATCDLDE
                     LVGLCTEPK"
     gene            1392..2351
                     /locus_tag="PLQ68_04170"
     CDS             1392..2351
                     /locus_tag="PLQ68_04170"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HXK71881.1"
                     /translation="MIQTIYMNSPLVITPFKKRLITYAFHDIDGTHSLIRDWPPVMSI
                     VLNDVIENGLEQGYDSNENVKRLVINAGTKPLPETDRFCVESAGLSALTQMEWAIRRA
                     VEEKTINIECDMAMNSEKIKLIWEGKEELDYLAETEEMKKMLESNTPRLFKYYERVLN
                     GYCRDKNLVLARKDPESFRIKGSFKFLKYLKDNGVKNYFVTGAVVQKGMGMFEEVETL
                     GYKIGPDKLIEDIIGSTWTDKIPKNVIMNSLLERYGVKGENTLVVGDGRSEIKAGVDM
                     NSFTISRLSENMHYQKQLHIKMGTNMIVEDFEKEDIYKILSSE"
     gene            complement(2478..3500)
                     /gene="rsgA"
                     /locus_tag="PLQ68_04175"
     CDS             complement(2478..3500)
                     /gene="rsgA"
                     /locus_tag="PLQ68_04175"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HXK71882.1"
                     /translation="MFEEYGKKPYRNTARITAVHKQRYEIEYNGETCFAKLKSSEYYS
                     PDKYADFPTVGDYVEIFTVPNSDCMITKTHPRKSFFSRRDPSSAGHSDQIVAANFDYV
                     FILMSLNMDFNLRRLERYLTASWNSNALPVVLLTKADLCEDFDDMLLQVEQIAAGVDI
                     IPISSYTGYGLEELDKFLRPDVTIVFLGSSGTGKSTLVNALAKQQIMEVKNIREDDSK
                     GRHTTSHRQMIRLNSGACVIDTPGMRELGIWYAGEGLSETFSDILPLLGKCKFSDCAH
                     KTEPGCAIKEAIKEGIITQERWNSYLKIKKEARFAEDYESAIREKEAFFKSIAIKSKR
                     LNKRRY"
     gene            4061..5155
                     /locus_tag="PLQ68_04180"
     CDS             4061..5155
                     /locus_tag="PLQ68_04180"
                     /inference="COORDINATES: protein motif:HMM:NF012516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004650 - polygalacturonase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28 protein"
                     /protein_id="HXK71883.1"
                     /translation="MDFYVFEFLQEGMTDSECIKECIETANQHNKNKRVIFNRKDWLI
                     DEAILVSSDMEIVIDGCTIKQNDYVFDNIFRGVNLKVNDDDPYGKPEYVTPLKNITIK
                     GYNNAIIAGCDRSKTGYHPVLNKEMEMVGDNWGWRTILICISNCENIEITGLNIYQTK
                     CWAISLDFCSYCSIHDIYIESDVKNGDGIDLRIGCHHVRIWNITGSTSDDTVACTALF
                     HSRREYPHKNYLYPLAPMGAIFNGSKSDMDIHDVLISDIRTGGLCHGVICLAADGLQV
                     YNIDIKNIEDVKEGRRTATVMLYTGYGENYSDNDLHDIRISGIHSRFSKYAVSINTKV
                     NNVTVSDTVQENKEGNLYEFANPEGITVIE"
     misc_binding    5554..5766
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5821..7803
                     /locus_tag="PLQ68_04185"
     CDS             5821..7803
                     /locus_tag="PLQ68_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010076455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HXK71884.1"
                     /translation="MKEDIKRPIFPKRAVITAGMPYGNKELHFGHIGGMFVHADTYAR
                     FLRDRIGAENVIFVSGTDCYGSPILESFRKLKESGNTEHKDITEYVESNHIKQKKTLD
                     EYEISLNHFGASGLYKDKEIHRQVSNEVFETLYKSGYLSVMSTPQFFDPKLQILLNGR
                     QVEGRCPFEGCGSDKAYADECALGHQYMPEELIDPKSTLSGLKPEIVDVENWYFRLED
                     FRDKMQEYTKNLREDSVTRKFIIDAIDEFLKPPVIYVQRKQIDDLEDLEKAFPKHETI
                     DEEKKSSITFVFENLDDRDIARDVLNERQIRYRTGKTLVPFRLSGNIEWGVPVPKKDN
                     LEDLTFWVWPESLWAPISFLKAYLKEKGAKEDEWKKWWQDEDSMVYQFIGEDNIYFYC
                     IAEMAMFAVMNHPDFDTLDMTKIHLPQIIANKHILFMDKKASSSSQIKPPMANDLLQF
                     YTPEQLRMHFISLGLTSKSSSFKPQALLPKEEQEGVDPALKEGNLLTNVFNRLIRSCL
                     YTIQKYNDSKVIIGKVSEEIIDASDDAILEYEKHMYNNDIHRIAYVLDSYVRIINKHW
                     ANSIRTAEANDDERLRNQILTDCFHGIKTALLLLHPIAPTGCEKAKEYIGFSDKLWSW
                     DYAFSSVEELLIDKDAKTTFLEPKEDFFRKHESQFE"
     gene            7866..8315
                     /locus_tag="PLQ68_04190"
     CDS             7866..8315
                     /locus_tag="PLQ68_04190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK71885.1"
                     /translation="MKRFTALLLLISVLVMALTGCNNTVTMNKSPYSADIQDNRVSMT
                     VKEMDIKVSTDLVTLVMKNALGVDIYFGHEPHLEKLIDNQWYVVPLNDKAGWTEEEVV
                     LGGRGTVEEKFYLTFYDDLSKGEYRVIKNFYMDDGSKIMASVELNIN"
     gene            complement(8423..9103)
                     /locus_tag="PLQ68_04195"
     CDS             complement(8423..9103)
                     /locus_tag="PLQ68_04195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK71886.1"
                     /translation="MKDFISKVIPVSLSFGISYTMFIIIRDLKTIRLGDYDTPKVIRD
                     GNLSMFKSIIISNYLLRGLLIIFISILLIKVLTLLFKNIEINSIVKVSGIIGSVFMIF
                     IGFYHFKNIPYTENDVYYTIESKAENFITDFTNKEYDKMVTNYVLNSNMNEFISKYLY
                     SQAMNRTIREYGQIREIEDPIKIHIGVYTIFSYPVIHEDNQVVYYNIAFNSKHEISSL
                     FTTLSLLG"
     gene            complement(9413..11350)
                     /locus_tag="PLQ68_04200"
     CDS             complement(9413..11350)
                     /locus_tag="PLQ68_04200"
                     /inference="COORDINATES: protein motif:HMM:NF017280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HXK71887.1"
                     /translation="MRKNISKPLIWLSVMLVILFVCMIFADLIQRDFNKVEVSEFYFD
                     AGNGEILTYKLYKPASATKDNKSPAVLLMHGYQNDKETNAAYSIELARRGIVALSIDE
                     YGHGGTSLGIRERGYTQYKFPNMEKTISGPERYLVMMNFSNMDFHDEQYSKGLKDSSM
                     GGRLAYEMLKQMDFVDVENLGITGHSMGTWSSWTVAADNPDHKCIVIQCGEVADTKYF
                     DDSIKFNNVLMLQAKYDEFNYFRDYQNTVAGLEKTPLRYNTFALQDNPVEWNTTYGSF
                     EDGTARRMELLITNHRLVTHDKNGMAASMDWFTEAFGITKTIDSYNQTYMIKEVLVLV
                     AMLCAVFSILPLMILILKIPFFKAVAQQLPDRPSRIKNTRQWWKGAVITVLIAGLSYP
                     FMTQLGHGLLPLPENIFKMTVGNGFVGWYLFLIIIMVITLNSTWKKSQKLGSGLDYYD
                     LGLGCEKNPDKLSWSLLGKSSLVTLLMIGFMYLLVTVTQSLFMLDFRFIWPFFKMFTI
                     ERFLQFLVYIPIFALFFLLNNAKIFGHMRQSRASEDTISSLLICWLKNAFVMIGGVFL
                     MVLIEYIPFFLEIGPGADLLFSSTFGGPFMSLMILFIPQVIIFSFLCTFINKKTGHVY
                     VSAFTVASLACWIVTGGSALF"
     gene            complement(11538..12350)
                     /locus_tag="PLQ68_04205"
     CDS             complement(11538..12350)
                     /locus_tag="PLQ68_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020072211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXK71888.1"
                     /translation="MSSQTNFNNFSDTLVSIKNIDKTFNKGSVNEVSLFKGFSLDIIN
                     NQFVSVVGSNGSGKTTLLNLICGNMETDTGKIFLKDRNITNLKEHQRAKFIGRVFQDP
                     SKGTCSTLTILENMSLADNKNIGYSLKAGVNKKRKDYYCSQLQLLKLGLENKLDQQVG
                     TLSGGQRQALALLISTMTPIDLLILDEHTAALDPKSSENVMELTDHVIKEKKITTLMV
                     THNLKYAVKYGDRIIMMHDGKCVIDAAGEKKQTYEISDLLKIFNEISIECGN"
     gene            complement(12328..13242)
                     /locus_tag="PLQ68_04210"
     CDS             complement(12328..13242)
                     /locus_tag="PLQ68_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016146360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXK71889.1"
                     /translation="MDVLIGILTNIFEEGFIYGIMAMGVYISYKVMDFPDLSVDGTFP
                     LGMCVTASMITAGADPLLACIVAFIAGSLAGCITGILNVKLKITDLLSGILVMTAMWS
                     VNLAITKGSAVLPFYNKSTIFNTGLVQILPESVQKYRVLIVAGLMMLIVKILMDLYLK
                     TKSGLLLRAAGDNPQFVTSMSRNPGVMKIIGLAIGNGCTALAGSILSQQNESANITSG
                     TGMVILSLASVIIGTSIFSKVKFMKATTMAVIGAIIYKMCLVAAMQLGLPTNYLKILM
                     AVIFTVALVSNNFFSKGRRKQNVQPNKL"
     gene            complement(13247..14227)
                     /locus_tag="PLQ68_04215"
     CDS             complement(13247..14227)
                     /locus_tag="PLQ68_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016146359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXK71890.1"
                     /translation="MKATKKFLALVLTVALIFTLVSCTDNTDMIKIGVIQYAPHPSLD
                     NCYTGFIEGLAEGGYEVGKNIEIDFQNAMGEMSNSDLIASTMVSSKYDMILGIATPSA
                     MSAYGAAKNTDIPVIFSAVSDAVAAGIVQSNEKPGYNVTGTSDVLPLEAQMQMIRAFL
                     PEATKIGIIYTTSEPNSVTHLAQFKELAKQYNFTIVEVGVTNASEVAAAVTTVISKGV
                     DCINNFTDNNVVNNLSTLIHAADAANIPVFGSEVEQVKNGCIASESIDYVELGRETGR
                     MAAKILDGEKASEMPVALISASEPVYNKKVAQRLNINLPDAYADAEDVGE"
     misc_binding    complement(14290..14535)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            14765..15151
                     /locus_tag="PLQ68_04220"
     CDS             14765..15151
                     /locus_tag="PLQ68_04220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK71891.1"
                     /translation="MRRFNKLAARIFLIMLVLSSVCILPSASSDFYESTYNNGNYICR
                     STLTTDYGRSKIEYEDSAILVTSELTISCRPVNGSQCVQLPTCRSIAYSYAEVIGNAD
                     IDVVFCSAIHNYLICSNPVYFVGLTP"
     gene            15148..16320
                     /locus_tag="PLQ68_04225"
     CDS             15148..16320
                     /locus_tag="PLQ68_04225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK71892.1"
                     /translation="MKQKNMRKVIIFILIAWFLVLASCNSDYSTPLNTDYTTETDISF
                     QGDINDKRYTSFRLSDGCSFSQFVETYEGYYYLKNFYVYYCSDGISKYVKLCSKPDCR
                     HSDNNCNAYTGANLIAYYDGYMYYVAIDQYDYCLFRMNMDGTNHVKIKSISTIQEKCT
                     GFFEHGYLYYYKGTIYGLLGNTNTVIYRTAVFDKSEGTIVVDTKDNGINVDDIFLFYP
                     HDEYIYFYTFSPDTYYSVCRYSMETSKWSDYVNTEMFFQSVLTDADKVYWYLNGDGFY
                     EYAYETGTIERVADTVGDGLYGVSYNDEYIYAYQYYEEKYSPIPPMLYVFDRQYNLID
                     NVSVDIPMLNYFSPFYLTELDDYVLLSTSINDSAPDYYIKKSEFGSGNITVRKIGD"
     gene            16657..18012
                     /locus_tag="PLQ68_04230"
     CDS             16657..18012
                     /locus_tag="PLQ68_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005364223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXK71893.1"
                     /translation="MEIKRDKYLNKLIKKKDNGRVKIITGIRRCGKSYLLFELYKNYL
                     LRNGISENQIIEMPLDEIDNIQYRNPFELNAYIKERITDKNKRYYIFIDEIQFCEEVS
                     NPYIKDSNEKVTFIDTVLGLMKISNVDIYITGSNSKMLSKEVLTQFRDRGDEIHVMPL
                     SYAEFWVAYQKIHTQVKSPISPIMINRNDAWRDYCSFGGMPYVLVLDTVEEKSQYLKD
                     LFDETYIKDIIERNNIRNDKEVLEILLDFVSSSIGSLTNPRKLENRFLSERKINISHS
                     TITKYLDLFIEAYIMDSAKRYDIKGSQYFDTPLKYYFTDIGLRNARLNFRQIEQTHIM
                     ENIIYNELINRGYNVDVGVVQYAKREKDNQGEEKKVRVQLEVDFVVNHGNKRYYIQSA
                     LNIDNPQKREKEVNSLNRIDDSFKKIIVVKDDIIPWYDEKGIQYVGVQEFLLNERAMD
                     I"
     assembly_gap    18241..18340
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <18341..20034
                     /locus_tag="PLQ68_04235"
     CDS             <18341..20034
                     /locus_tag="PLQ68_04235"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXK71894.1"
                     /translation="GLFSASLNILIAWTIGQMLDIAIGGKWSEAITFIILISVFVLGR
                     SFIGYTNSLTSYNYEAQAGYVMRCSAMDKINALPIGYFESKHTGTVISRMMNDIDKMQ
                     EFLPNSVAGIWSHTPVNMIFGLTLLFLIDWRLTLIVVSIVMITTFILSRISVPLVKFT
                     DAEQESKVKYNAYLRDFIEGNDIYKIYGMKRTHGKKFESAVDEQFECSKKINRQKSKM
                     NAITGINYYLPWVLSYVIGSFFVAKGDITIGALYSFAAILPNVTKVAWQIGNNLAAMV
                     ETSGIAKHYFELIDQPDERKNGQDFSSVNNNTIEFDSVSYSYSSGLSILSGCSFIVSA
                     ASTVALVGGSGSGKTTIFKLICGYYEEYHGDVKICGYPLREWNLHALRSSITYMSQES
                     YLFDDTIMENIRNGRPEATDNEVIDAAKAAYADEFIMQTVNGYNTVLGERGIMLSGGQ
                     RQRIAIARAMLRNTPIILLDEPTSSLDTRSEYHVQLALQNLTKDRTVFVIAHRLSTIE
                     KADTILVLENGRIIEQGPHLELVKRNGRYKELYSAQIAEVNANETQFLQGFHAIF"
     gene            19994..21739
                     /locus_tag="PLQ68_04240"
     CDS             19994..21739
                     /locus_tag="PLQ68_04240"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXK71895.1"
                     /translation="MKRNFFKDFMRFSKLCGSKSRRLLVIGILDGLVNGALGTIFQAQ
                     ALRYIIAYFIKKQSDALSKAFVMIGFTMLVGFVFRPLINYYSQVTISKITALLRMRLY
                     KHLTQLPTSYFTGTHSGDIAARINGDVEAFAWACNTLLNFLGAVIPAFVLMPYLLTLD
                     MRMALIIGVVGLLFLILTAKVIEPLRKRQRKIRTEISNMSVSAADGIMGFQVIKSNNL
                     EEQYSNKFKSHAEKLLDTQKDFGKVNAIVGFFNIFIWCAGEAFIAIFGLVFVLKGTLA
                     VTNLAALVSAGRSIIGIFTDFSSLPIQLQQAFAGVDRVYELCDILPEAERYLINGNRS
                     ECGICVKNLSFSYNNKTLVLDNISIYAQKGESIALVGDSGSGKTTLIKILAGLYRIDD
                     GEITVLNRPLSDYTLDEYRAKIAYVPQNAYIFDGSIAENICYGREGALLDEVKLAARK
                     ANAELFIMQKPAQYETQVGERGIQLSGGERQRIAIARAILKNSEILLLDEATSSLDSE
                     SELAIQDTLDNLIQQHTSVIVAHRLSTIVNADCIYMMQKGRIIGSGKHEQLLTSCSEY
                     KQLYYRLFASKKDQE"
     gene            21879..22913
                     /locus_tag="PLQ68_04245"
     CDS             21879..22913
                     /locus_tag="PLQ68_04245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK71896.1"
                     /translation="MKNYELITKWVIDTVKKDYFDDIALVVSHTTLRIDESEQTISYF
                     VPITEKGRKFGQTFILQGEGFDIWGIDWKRLEKFAELDEYNITVLADSKVLYSRTAED
                     EQHFAALKEKQLSNLSDMRKMRACALAAYAQARKIYFEMLFAQTESDIKFAAGYTIDY
                     LAQAVAFTNQKYFAYSQINQLNELKKMENVPARFSELYNSIIYEADVEKQKSLCLELI
                     NVVRIFLEDNKMNNDEKRSCTENNYQDLADWYTELSYTWLRIRKYAELNDVTKTYMWG
                     IMLQAELNRVCDDFGIDKYELMSNFNSENLSGFAAESNAIECNIRKLITENGGIIREY
                     NSFEDFVNEV"
     gene            22903..23166
                     /locus_tag="PLQ68_04250"
     CDS             22903..23166
                     /locus_tag="PLQ68_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018757228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3324 family protein"
                     /protein_id="HXK71897.1"
                     /translation="MKYKNAQEILPDKLLKELQKYISGGAIYVPNIESKKQWGEQSGA
                     RCYYKQRNEEIRENHKNGKSLDELSFEYGLSIESIRKIVYSTK"
     gene            complement(23317..24663)
                     /locus_tag="PLQ68_04255"
     CDS             complement(23317..24663)
                     /locus_tag="PLQ68_04255"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HXK71898.1"
                     /translation="MKLYSREWLIENNYPYGKAFEGIPKGDPLIEYKRTSPDVILYNP
                     DGIEYYKGDNEHLLVFESPDQLELLAVWTQSSCEGKGNNHIVMSRSKDSVNWTSPVYI
                     AGTKENERFNQASWAFPIVTSKKRIYLFYTKQLELSDNHHAGSGGLGCIYSDDYGYTW
                     SDEAVLPMARSRFDNPDTKYPKNWIVWQKPIKDANNKYIAGYTLCTSHVHNISDYDKT
                     RWVNQDSRCYFMRFENIDDNPDPYDIEITWLPDDDKGIEVDNPIIPNMSTAQEPAITL
                     LPDGRIFCIMRTVTGYVYYSTSEDNGHTFKKAQPLRYGDNLEYVKHPLSPAPIYSIKD
                     GLYLTAFHDNDGERIGFSQKKENWEYNMSNFIRNPLYIAIGRFDAKAEQPIRFGMKYK
                     LLDTEDIAVGPKNTSETGTYTSMTYFKNKLMFWYPDRKYYLLGKQITDELIKQIAP"
     gene            complement(25039..26943)
                     /locus_tag="PLQ68_04260"
     CDS             complement(25039..26943)
                     /locus_tag="PLQ68_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXK71899.1"
                     /translation="MTQQQKRHVPQVDRFQKPKNTKKTFKRLLDYLFAYRTRLIFVII
                     LVLLSAAGTVAASYLLKPLINDYIAPLTGTNPTGEDYLPLIKFLMVMAGIYLSSVIAS
                     YLYNYQMMKVTNSVLKNLRRDLFNKIQDLPIKYFDTHTHGEVMSRFTNDVETFRQAIS
                     NGFVQFISSSVSVVGIFTLMLVLSPILTLIIVITLAVMFLIIKKIGSNSAKFFKKQQK
                     ELGEVNGFVEEMIEGQKVVKVFCYEDNIVEDFNKKNENLRIAATDANTYANIIMPIMG
                     NLGHLNYAATAAVGAILCINGMLDIGSLASFLQFTRQFSQPITQISQQINAILAAFAG
                     AERIFEVLDQPKEFDEGYVTLVNVCKDGNDMTECSTQTGHWAWKHINNDNTVEYIELK
                     GDVRFNNVTFAYNEGTDVLKNVTLYAKPGQKIAFVGSTGAGKTTITNLINRFYEIQQG
                     EITYDGIDIKKIKKEDLRRSLAMVLQDTHLFTGTIRENIRYGRLDATDIEVEEAAKLA
                     NADSFIKYLPEGYDTLLTSDGANLSQGQRQLLSIARAAVANPPVLILDEATSSIDTRT
                     EALIEKGMDKLMEGRTVFVIAHRLSTVRNSNAIMVLENGQIIERGDHENLLKQEGRYF
                     KLYTGQFELS"
     gene            complement(26945..28672)
                     /locus_tag="PLQ68_04265"
     CDS             complement(26945..28672)
                     /locus_tag="PLQ68_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019555152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXK71900.1"
                     /translation="MISKLLPYAKKYWLQTFLTPLFIIAEVLLEVNIPFIMAKIVDVG
                     IANSDIGYIVNQGLLMVLMALLSLGCGMIASRLGAVAGIGFGAELRRGVFSKIQDFSF
                     SNMDQFTTASLISRSTQDITQIQNAFNMVLRMAVRSPFMFISALIMAIRINKSLVSVF
                     LVVIPILVGVIIIISTKAFPRFQEMMKKYDTINSSVQEDLIAIREVKAFVRSGHEKEK
                     FNKTNNENMAASLHAEKLLITTMPLMMLAMYSCMIAILWLGGKQIIIGQMLTGELISF
                     ISYIGQILMSLMMLSFIFIMLIMSRASRVRVAEVLDTVSDLTDKDSDKELSVESGDIE
                     FKNVFFKYSKKNENYILEDINLIINKGETVGIIGSTGSAKTTLIQLIPRLYDADTGEV
                     LVGGRNVKDYSLKTLRDAVSVVLQKNTLFSGTILDNLKWGNPDASTEQIEKACKIANA
                     HDFIMSFPDGYMTNLGQGGVNVSGGQKQRLCIARALLKNPKILIMDDSTSAVDTATDA
                     SIRNELKKNTSDTTVLIIAQRITSVYDADKIIVMNDGKIDAVGTHEELLKTNTIYSEV
                     YYSQQKGVA"
     gene            complement(28669..29163)
                     /locus_tag="PLQ68_04270"
     CDS             complement(28669..29163)
                     /locus_tag="PLQ68_04270"
                     /inference="COORDINATES: protein motif:HMM:NF013231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HXK71901.1"
                     /translation="MEHKTTLQKLGKITLLHKLYFNKAAENKDIYLGQLPIIHYIMSN
                     DGCTQKDLVSFFKVSAPSIATSVKRLMKSGYINRLTDESDSRVNRLHATKKAHDISTA
                     MKKECEAIDEMAFRDFSDNELLEFERLINKILDSLNTEEFKTKNIHELISEEKKIHKK
                     GGLG"
     gene            complement(29366..29635)
                     /locus_tag="PLQ68_04275"
     CDS             complement(29366..29635)
                     /locus_tag="PLQ68_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006002014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HXK71902.1"
                     /translation="MNYHVVFSERARRELKKLDHYTAAMIMGWIQKNIEGCSNPRQFG
                     KELKANLSGQWRYRIGNYRLIAEIQDNTVLILILHIGHRSEIYNK"
     gene            complement(29632..29853)
                     /locus_tag="PLQ68_04280"
     CDS             complement(29632..29853)
                     /locus_tag="PLQ68_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008750290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="HXK71903.1"
                     /translation="MTISLRLNEADTMLFRKYAEMKGISVSELIRQTVLERIEEEFDL
                     KVYETAMTEYRTNPTTYTMEEMEKELGLR"
BASE COUNT         9505 a         5092 c         5609 g         9626 t
ORIGIN      
        1 tagtcagata attgcttttt tttagatcgt ataggctttt tgcctgtttg tttaatacca
       61 aggaaaaacc gcccctttat agtgccacta attatatcag cattaaaaaa aaatgcaagc
      121 atgaaaaaaa gaaattatat tcattcattt tgaataatat acattgcttg aacacaataa
      181 ataataaaca agggtataat accgtgtaat tcttgttgat acaatgtatc aattatgaat
      241 attacttatt ttcactataa aaaaaatatt ttatatttaa cagaatgttg acttaagagg
      301 gtaatttgta tacattagag gtagtaattc tactatatat aaaaggaagg tattttaaat
      361 gttagatgaa gcaacccgca agatagtaca cgcatgtccc aaggaagcat tggatgaaag
      421 cctgttttct accagctcaa taataacaag atatccagaa acaattctga gcaggcatga
      481 tgtaccatat cccgctgcct tgacattcaa tgcaggagtc gtcaaagagg gcgacagata
      541 tataatgatt ttccgtaatg attacggttc atatgaggat cagagaatag atggaactaa
      601 tctgggtctt gcttttagta aagatggtat taaatgggat gttgaaccag aaccatgttt
      661 tgctatggca gatgatgaag tgttaagagt ctacgatcca agaataactg ttatggatga
      721 cagatatata atttgttttg cagtagacac aaaacacgga gtatgtggtg gtattgcaga
      781 aacaaaagat cttaagaaat ttgatgttgt atcaatatca gttcctgaaa acagaaacat
      841 ggttcttttc ccagaaaaga taaacgggaa ttatgtacgt cttgaaagac caatgcctgt
      901 atatggcaga gatggaatga aatttgatat atttctttcg gaatctcctg atcttgttta
      961 ctggggaaaa tccagacttg ttatgggagt tgagcatgtc ccatttgcaa acgaaaagat
     1021 tggtccggga gctcctccga ttaagacaga caaaggatgg gtaatgctgt tccacggtgt
     1081 tgattttgat cctgcaagag gaaagaatgg atgggaagaa acatggaaga agagatatac
     1141 gataggtgtc gctttacttg atcttgaaga tccttcaaaa gttataggaa tgtacaaacg
     1201 tccgctaata attccagaag ggtattatga gcttatagaa ggctacagaa ctaatgcaat
     1261 attcccatgc ggagcgcttg ttgacgacaa taatcaagta aggatttatt atggagccgg
     1321 agatgcgata atacgtatgg caacatgtga tttggacgag cttgtaggtt tatgtacgga
     1381 acctaaataa tatgattcaa acaatttata tgaattcacc attagttatc acgcctttta
     1441 aaaaaaggct gataacttat gcttttcacg atatcgacgg gactcattcc ttaataaggg
     1501 actggcctcc ggtaatgagt attgttctaa atgatgtaat agaaaatggt ttggaacaag
     1561 gatatgacag caatgagaat gtaaaaaggc ttgttattaa tgctggtaca aaacctttgc
     1621 ctgaaacaga cagattctgt gtagaatctg ccggtctttc tgcgcttact cagatggaat
     1681 gggcaataag aagagctgtt gaagagaaaa ccatcaatat tgaatgcgat atggcaatga
     1741 acagtgaaaa gataaagctc atatgggagg gtaaagaaga actcgactat cttgctgaaa
     1801 ctgaagagat gaaaaaaatg cttgaaagta atacacccag attattcaaa tattacgaaa
     1861 gagtattaaa tggttactgt agagataaaa accttgttct tgcaagaaaa gatcctgaaa
     1921 gttttagaat taaaggttct ttcaaatttt taaaatactt aaaggataat ggagttaaga
     1981 actattttgt tacaggagca gtagtgcaaa agggaatggg tatgtttgag gaagttgaaa
     2041 cattaggtta caaaataggc ccagataagc ttattgagga tataattgga tccacttgga
     2101 cagataaaat tcctaaaaac gtgattatga atagtctttt agaaagatat ggagtaaagg
     2161 gagaaaacac cttagtagta ggggatggaa gatcagagat taaggctgga gttgatatga
     2221 actcttttac aattagcaga ttgtctgaaa acatgcatta tcagaaacag cttcatatca
     2281 agatgggtac taacatgatt gttgaggact ttgaaaagga agatatatat aaaatactgt
     2341 cttctgagta agtttattta agcagaacat tggtgctgat tgaagtaaaa aatataaaca
     2401 gttaacaaat taagtattgc atataaagga aacgaatact catctgtatt cgtttccttg
     2461 tatatttctt tttaatttta atatcttctt ttatttaaac gtttgctctt gattgcaatg
     2521 cttttaaaga atgcttcctt ttctcttatt gcactttcat agtcttcagc aaagcgggcc
     2581 tcttttttaa tcttcagata actgttccat ctttcctgtg taataatacc ttctttaata
     2641 gcttctttta ttgcacagcc aggttcggtc ttgtgcgcgc agtcagaaaa cttgcatttt
     2701 ccaagaagag gaagtatatc agaaaatgtc tcacttaatc cttcaccagc ataccatatt
     2761 ccgagttctc tcatgcctgg ggtatcgatt acacatgctc cgctattaag ccttatcatc
     2821 tggcgatggc ttgtagtatg tctgcctttt gaatcatcct cacggatatt tttaacttcc
     2881 attatctgct gctttgctaa tgcgttcact aaggtcgatt tacctgtacc ggaagatcct
     2941 aaaaagacta ttgttacatc aggacgaaga aatttatcca attcttccag accataacca
     3001 gtgtaggaac ttatcggtat gatgtctaca ccagctgcaa tctgttcaac ctgtaataac
     3061 atgtcatcaa aatcttcaca taagtcagct tttgttaaca aaacgacagg tagggcattg
     3121 ctattccatg atgcggtaag atagcgctcc agacgtctta aattaaaatc catattaaga
     3181 gacattaaaa tgaaaacata atcaaaattc gctgcaacta tctggtcaga gtgaccagca
     3241 gatgaagggt ctcttctgga aaagaatgat tttctaggat gagtcttagt tatcatgcag
     3301 tcagagtttg gcactgtaaa tatttccaca tagtctccca ctgttggaaa atctgcatat
     3361 ttgtctggcg aatagtactc tgagctttta agctttgcga aacatgtttc accattatat
     3421 tcaatctcat atctttgttt atgtactgct gttattcttg ctgtatttct atatggcttt
     3481 ttcccatatt cttcaaacat cgttgtttcc tccaaattaa atttattatt gttttgatgt
     3541 ttaccgaatg agccttttaa ggaactaaaa ggacacccat atgagtgtcc aaattttaag
     3601 ataaaattta ctttttattc atagataggc tgctttgtgc ttatgctata tgcaataagc
     3661 caatattcgg ttttagattt gtactacaat atcctttcta atcataaaca cgcaacctcc
     3721 tttatctttt tacaatatat cagcaataat tatcggtgtc aattactagt tattgtaaaa
     3781 tcaaaaatca atgttggaat agcaataatg caataggtgg aaattataat atttagtatt
     3841 gttttaataa catttacaat tatttaattg acctggacat ggtttataaa ataatattat
     3901 agcagacaaa tcccgacttt gataatttaa actgtgtatc accgtattat ttttgatact
     3961 attcctctct ttagacaaat ggcccttcat gggtcatttg tcttatataa accattgctg
     4021 tatacttaaa taaacatatt tttttaagtg agggtttgtc atggattttt atgtcttcga
     4081 atttctacaa gagggaatga ctgattcgga atgtattaaa gaatgtattg aaactgctaa
     4141 tcaacacaat aaaaataaaa gagtaatctt caacagaaaa gactggttga tagatgaggc
     4201 cattttagta tctagcgata tggaaatagt aatagatggc tgtactatta agcagaatga
     4261 ttatgttttt gataacattt tcagaggagt caatcttaaa gtaaatgatg atgatcctta
     4321 tggaaaacct gaatatgtta ctcctttgaa aaatataaca ataaaaggat acaacaatgc
     4381 aataatagca ggttgtgaca ggagtaagac aggatatcat ccagtgttga ataaagaaat
     4441 ggaaatggtt ggggataact ggggatggag aaccatttta atatgcatat ccaactgtga
     4501 aaatatcgag ataactggtt taaatatata tcagacgaaa tgctgggcga tttctttgga
     4561 tttctgttcc tattgcagta ttcatgacat ttatattgaa tctgatgtaa agaacggaga
     4621 cggtattgac ttaagaatag ggtgtcacca tgtcaggata tggaatatta caggaagcac
     4681 atcagatgat actgtagcat gtactgcgct gtttcatagc agaagagaat atccgcacaa
     4741 aaactatctt tatcctctgg cgccaatggg agcaattttc aatggcagca aatctgatat
     4801 ggatatacat gatgttttaa tcagtgatat aagaacaggt ggattatgcc atggagtaat
     4861 atgtttagca gcagatggtt tacaggtata taacatagat ataaagaata tagaggatgt
     4921 aaaagaagga agaaggactg ctacggtaat gctgtataca ggctatggcg aaaactattc
     4981 cgataatgat ttgcatgata tacgaataag cggtattcat tcaagatttt ctaagtatgc
     5041 agtatctata aatacaaagg taaataacgt aactgtatct gatacagtac aggaaaacaa
     5101 agaaggaaac ctttatgaat ttgctaatcc agagggtata acagttatag aataaagcta
     5161 atcaactata tatgagtcta ttgagggttg ggtaatgaaa gaaaccatat accaaggaag
     5221 atatagaatc tttttttctc ttttaatgtt accagaacat aagactatgc cctctttttt
     5281 tcataatcat ttatagacat aagttttttc aaagctttat gagcagaata agctttccct
     5341 gatttgcatt ctattgatat gacggaactg ctttttagga ttttatcctt atttaaggca
     5401 taagttaata tgcagtgtaa ccttttttcg gagctaaaac aggcaaaatg caaccttttt
     5461 ccgaaattag atgtacttgt gtacttgtca agtagtcaag tggacataaa gttatatttg
     5521 acataatctg taccttgttc tatactttct gtaataaggg cgatgataag ataagtagtg
     5581 tgaagaatgg atttacagag agccttgaag ctgagaaagg gtattgcata tcacacgaaa
     5641 caggccttgg agccgtgaaa accgggagtt cccgttacag gacataagta tgatgatact
     5701 taataaggcg tattcggtga cggatatgtg aaaaagggtg gcaccgcgat attaatcgcc
     5761 cctgataaaa caacagtttt atgggggctt ttttatagcc tccagaagga gtgaactaat
     5821 atgaaagaag acattaaaag accgatattt ccaaaaagag cagttataac tgcaggcatg
     5881 ccatatggca acaaggaact tcatttcgga cacataggcg gaatgttcgt gcatgcggac
     5941 acctatgcaa ggttcttaag agacaggata ggagctgaaa atgtaatctt tgtatctggt
     6001 acagattgtt atggttctcc aattctggaa agcttcagga agcttaaaga aagcgggaat
     6061 acagaacata aagatattac tgaatatgtt gaatcaaatc atattaagca gaaaaagaca
     6121 ttggatgaat atgagataag cctcaatcat ttcggagcat caggtttgta caaagataag
     6181 gaaatacaca gacaggtatc aaatgaggtt tttgaaactc tgtataaaag cggatatctt
     6241 tcagttatgt ccacaccgca gtttttcgat cctaaattac agattctttt aaatggcaga
     6301 caggtagaag gcagatgtcc atttgaagga tgcggttcgg ataaagctta tgcagatgaa
     6361 tgcgcgcttg ggcatcagta tatgccagaa gagcttatag atccgaaaag tactttatca
     6421 ggcttgaaac cagagatagt tgatgttgag aactggtatt tcaggttaga agacttcaga
     6481 gacaaaatgc aggaatacac aaaaaactta agggaagatt ctgtaacaag gaaattcata
     6541 attgacgcta ttgatgaatt tttgaaacct cctgttattt atgtgcaaag aaagcagata
     6601 gatgatttag aagatttaga aaaagctttt ccaaaacatg agactataga tgaggaaaag
     6661 aaatcttcaa ttacatttgt ttttgaaaat cttgatgacc gtgacattgc aagggatgtc
     6721 ttaaatgaaa gacagatcag ataccgtaca gggaaaacac tggttccttt ccgtctttca
     6781 ggaaatattg aatggggagt tccagtaccg aaaaaagata atcttgaaga ccttaccttc
     6841 tgggtatggc ctgaatcgtt atgggcacct atatcatttt taaaagcata tcttaaggaa
     6901 aaaggtgcaa aagaagatga gtggaagaag tggtggcagg atgaagattc aatggtttat
     6961 cagtttattg gagaagataa tatatacttc tactgtattg ctgaaatggc aatgtttgca
     7021 gtaatgaatc atccggattt tgatactctg gacatgacca agattcatct tcctcagata
     7081 attgcaaata agcacattct gtttatggat aaaaaagcca gcagcagttc gcaaatcaaa
     7141 ccgcctatgg caaatgacct attacagttt tatactcctg aacagttaag gatgcatttc
     7201 ataagcctcg ggcttacaag taagagcagt tcctttaaac cacaggcttt gcttccaaaa
     7261 gaagagcagg agggggtaga ccctgcatta aaagaaggta atcttcttac taatgttttt
     7321 aacagactta taaggtcatg tctgtataca atacagaaat acaatgactc aaaagtaatt
     7381 attggtaagg tatccgaaga aataatagat gcttcagatg atgcgatact ggaatatgag
     7441 aagcacatgt ataacaatga tattcacaga attgcatatg tactggacag ttatgtaagg
     7501 ataataaaca aacactgggc taatagtata aggacagctg aagctaatga tgatgaaagg
     7561 ttgagaaatc agatattaac agactgtttc catggtatta aaacagcgct tcttctttta
     7621 catcctattg caccaacagg atgtgaaaaa gcaaaagaat acataggttt ttctgataaa
     7681 ctgtggagct gggattatgc attttcttct gtggaagagc tgcttattga taaagatgca
     7741 aaaactacat ttttagaacc taaagaagac ttcttcagaa agcatgaaag ccagttcgaa
     7801 taagagcata gattagggaa cctttaatca ttaatatgtg cctaaaaaag gaaaggatgt
     7861 aaaacatgaa acggtttaca gcattactcc ttttaatatc ggttcttgta atggcactta
     7921 cagggtgtaa taacactgtt acaatgaaca aaagcccgta tagtgccgat atacaagata
     7981 acagagtgtc aatgactgtt aaggaaatgg atataaaagt ttctacagat ttagttactc
     8041 tggttatgaa gaatgcatta ggagtggaca tatatttcgg tcatgagccg caccttgaaa
     8101 agctgattga caatcaatgg tatgtagtgc ctctgaatga taaagccgga tggaccgagg
     8161 aagaggttgt tttgggagga aggggaacgg tagaggagaa attctattta accttctatg
     8221 atgatttgtc aaaaggagag tatagggtta taaagaactt ctatatggat gacgggtcta
     8281 aaattatggc atcagtggaa cttaacataa attagctgaa agtacgacaa aagatgacct
     8341 ttataaagca caaaagatat agatcagctg catacatcat gtatgcagct tttttaggtt
     8401 taaatttata aaaaaagtct gttcaaccaa ggagagaaag agtagtaaac aaactggata
     8461 tttcatgttt gctgttaaaa gcaatattat aatatactac ttgattatct tcatgtatta
     8521 caggataaga aaaaatagta tatactccaa tatgtatttt aataggatct tctatttctc
     8581 ttatttgacc atattctctt atagttctgt tcatcgcctg agaatacaag tacttgctga
     8641 taaattcatt catattactg ttaagaacat aatttgtaac cattttatca tattctttat
     8701 tggtaaaatc ggttatgaaa ttttctgctt tactttcaat agtgtaataa acatcatttt
     8761 ctgtatatgg tatattttta aagtgataaa agcctataaa tatcataaaa acggatccta
     8821 tgataccact aacctttact attgaattta tttctatatt cttaaataaa agtgttaaaa
     8881 cttttatcaa taaaattgaa atgaagataa ttaaaagacc tcgtaatagg taattactaa
     8941 taattattga tttgaacata cttaaattac catctctgat aacttttggc gtgtcataat
     9001 caccaagtct tattgttttt aaatctcgaa taatgataaa cattgtataa ctgataccaa
     9061 aggaaagtga cacaggaata acttttgata tgaagtcttt cataataaac tctcttttct
     9121 aaaaatatta tttgcaataa ttattattac acattattca ttatatttta agtgttttca
     9181 aacaattctt ttaataaatt aaccctttaa gtcaaaatac catcttatga actccaggat
     9241 accttcatca ctgggttaca agaacccaat tgaatacagg aatgataccc ttaagaaagt
     9301 tatctaaaaa agtgttgcct atccaccaat caattctgcc tatatatatt atatataaat
     9361 actatatata taatatataa attattcatt atatgaacag aatagcaaaa ccttaaaata
     9421 gtgccgaacc accagtcact atccagcagg caagtgatgc aacagtaaat gcgcttacat
     9481 atacatgacc tgtctttttg tttataaacg tgcacaggaa actgaatata ataacctgag
     9541 gtatgaacag tatcattaat gacatgaaag gcccgccgaa tgtggagctg aataaaaggt
     9601 ctgctccagg tccgatttcc aaaaagaaag gtatatattc aataagtacc attaagaata
     9661 ctccgccaat cataacaaat gcatttttta accagcatat cagcaatgaa cttattgtat
     9721 cttcagatgc tctggattgt ctcatgtgtc cgaatatttt tgcattattc aaaaggaaga
     9781 ataaagcgaa aattgggata tagactaaga actgaaggaa ccgttctatt gtgaacatct
     9841 taaagaaagg ccatatgaat cgaaagtcaa gcataaaaag cgactgagtt acagttacaa
     9901 gaagatacat gaagccgatc attaataatg ttactaatga tgacttgcca agtaatgacc
     9961 aggaaagttt gtctggattt ttttcacacc ccaatccgag atcataataa tccaacccgc
    10021 ttccaagttt ctgagatttt ttccaggtgc tgttaagtgt aatgaccata ataattatca
    10081 ggaacagata ccagccgaca aaaccgttgc ctacagtcat tttgaatata ttctctggta
    10141 atggcagtaa tccatgacct aactgtgtca tgaaaggata ggaaagccct gctatcaata
    10201 cagttataac tgcacctttc caccactgtc gggtattttt aatccttgaa ggcctgtcag
    10261 gtaattgctg tgctacagct ttaaagaaag gaatcttaag tattagtatc attaatggaa
    10321 gtatagaaaa tactgcacat aacattgcga ctagaaccag cacttccttt atcatatatg
    10381 tctgattgta tgaatcaatt gtttttgtaa tcccgaaagc ttcagtgaac cagtccatag
    10441 aagcggccat gccgttttta tcatgggtta caagtctgtg attggttatt agaagttcca
    10501 ttcttcttgc agtcccgtct tcaaacgagc catatgtagt attccactca acaggattgt
    10561 cctgtaatgc gaaggtattg tatcttaaag gagtcttctc aagtcctgca accgtattct
    10621 gataatcacg gaaataattg aattcatcat acttagcctg tagcataagt acattattga
    10681 attttatgga atcgtcaaag tatttagtgt ctgctacttc tccgcactgt atgactatgc
    10741 atttatggtc agggttatca gcagctaccg tccatgagga ccaggtaccc atggaatgac
    10801 cggttatgcc cagattctcc acatcaacaa agtccatctg cttgagcatt tcataagcca
    10861 gccttccgcc catagagcta tctttcaagc ctttgctata ttgctcatca tggaaatcca
    10921 tattggaaaa attcatcatt acaagatatc tttctggtcc tgaaatagtc ttttccatat
    10981 ttgggaattt atactgtgta tatcctcttt ctctgatgcc taaacttgtt ccaccatgac
    11041 cgtactcatc aatagacaag gccactattc ctcttcttgc aagttctata gaatatgctg
    11101 cattggtctc cttgtcattt tgatatccat gcattaaaag tacagcggga gatttattgt
    11161 cttttgtagc actggccggt ttatacagtt tgtatgtcag tatctcacca tttccagcat
    11221 caaaatagaa ttctgaaacc tcaactttgt taaaatccct ctgtataagg tcagcgaata
    11281 tcatgcaaac aaaaagtatg acaagcatta cgcttaacca gattaaaggt tttgaaatat
    11341 tctttctcat aaaactaccc ctttcgaaat gtctgtttag caaaaatgga ctatttctgt
    11401 tgctaattat atatttgtta aatctaaatg taatattaac actttttgtt attatctaca
    11461 atattagata aaagtaaaac ggcacgcatt cttgatgaat tacgtaccgc tttatcaaaa
    11521 tgaggaagtt atgactgtta gttaccgcat tcgatggata tctcgttaaa tattttaaga
    11581 aggtcagata tctcataagt ttgctttttt tcacccgctg catcaattac gcatttaccg
    11641 tcatgcatca ttattattct gtcaccgtat ttgacagcat atttaaggtt atgagtaacc
    11701 atcaatgttg ttattttctt ttcctttata acatggtctg taagctccat tacattttct
    11761 gacgatttgg gatcaagtgc tgcagtgtgt tcatcaagta taagcaggtc gatgggtgtc
    11821 atggtggata tgagcaaagc taaagcctgt ctctgacctc cgcttaatgt cccgacctgc
    11881 tggtcaagtt tattctccag ccccagcttt aaaagctgaa gctgcgagca gtagtagtcc
    11941 ttgcgttttt tattgactcc tgcttttaag ctgtaaccta tgttcttgtt atcagctaaa
    12001 gacatattct caaggattgt aagtgtggaa caggttcctt tggaggggtc ctggaatact
    12061 cttcctataa atttagctct ttgatgttcc ttaagatttg ttatattcct gtcttttaaa
    12121 aatatcttgc ctgtatctgt ttccatgttt ccgcagataa ggtttaaaag agttgtcttg
    12181 ccggaaccgt tgcttcctac aacggatacg aactggttat ttataatatc caggctgaag
    12241 cctttaaaca agctgacttc atttactgaa cctttgttaa aggttttatc gatattcttt
    12301 atagatacaa gggtatctga aaagttatta aagtttgttt ggctggacat tctgtttcct
    12361 ccttcctttt gaaaagaagt tgtttgatac taaagcgact gtgaatatga cagccattaa
    12421 aatttttaag taattagtcg gaagccctaa ctgcattgct gctactaagc acatcttgta
    12481 aatgattgcc cctattactg ccatagtcgt ggctttcata aactttactt tactgaagat
    12541 gctggttcct ataataactg aggctaaaga taatataacc attccagtac ctgatgttat
    12601 gttcgctgac tcgttctgct ggcttagtat cgaccctgct aaagctgtgc agccgtttcc
    12661 gattgcaaga cctataatct tcattactcc tggatttctt gacattgatg ttacaaactg
    12721 aggattatct ccggctgctc ttaacaaaag accggatttt gttttaaggt ataggtccat
    12781 cagtatttta acaataagca tcattaatcc tgcaactata agtactctgt atttctgaac
    12841 agactctggc agtatctgta caagtcctgt gttgaatata gtgcttttat tgtagaaggg
    12901 gagcacagcg cttccttttg ttattgcaag atttacagac cacatagctg tcataaccaa
    12961 aatacctgat aacagatccg ttattttcaa cttaacattc aatatgcctg ttatgcatcc
    13021 agctaaactt cctgcaataa atgccacaat acatgctaat aatgggtcag ctcctgctgt
    13081 tatcatagag gcggtgacac acatccccaa agggaatgtg ccgtcaaccg acaggtccgg
    13141 aaaatccatg actttgtaac ttatatatac acccatagcc atgattccgt atatgaatcc
    13201 ttcttcgaat atgtttgtca gaataccaat caacacatcc attaaactat tctcctacat
    13261 cctctgcgtc ggcatatgca tcaggcagat taatattcag tctttgtgcc acttttttat
    13321 tgtaaacagg ttcgcttgca gatatcagtg cgactggcat ttctgatgct ttttcaccat
    13381 ctagtatttt tgctgccatt ctacctgtct cacgtccaag ctccacataa tctatgcttt
    13441 ctgaagctat acatccgttt ttgacctgtt ctacttcaga accgaaaacc ggtatgtttg
    13501 ctgcatcagc tgcatgtata agggtagaaa ggttattaac tacattgttg tcagtaaagt
    13561 tgtttatgca gtccacgcct tttgatatta cagttgtaac tgccgctgcc acttctgatg
    13621 catttgtaac gcctacttca actattgtga agttatactg tttggcaagt tccttgaact
    13681 gggcaagatg agttactgaa ttcggttcgc ttgtagtgta gataattccg attttggtag
    13741 cttcaggcag gaatgctctt atcatctgca tctgtgcttc taaaggaagt acatcagatg
    13801 ttcctgttac attgtaacct ggtttttcat ttgactgtac tattcctgca gcgactgcat
    13861 cagacactgc tgaaaagata accggtatat cagtattctt tgctgctcca tatgcgctca
    13921 tagctgaagg agttgcaatt cccagtatca tatcatattt tgatgacacc atagtggaag
    13981 caatcaggtc ggagtttgac atttctccca tggcattttg aaagtcgatc tcaatattct
    14041 ttccgacttc atagccgcct tctgcaagtc cttcaataaa ccctgtataa caattgtcca
    14101 gtgaagggtg tggtgcgtat tgtattacgc cgattttgat catgtcggta ttgtctgtgc
    14161 atgagacaag tgtgaagata agtgctaccg ttaagaccag tgctaaaaat ttctttgttg
    14221 ctttcatttt tctttcctcc aaatgtttat tttatcccca aagaaaacaa aaaatccctt
    14281 gttcctattg ctagggacaa gagatttatc actcctgcgg taccacccag attggcttat
    14341 aaagcccact cgttccatat acttacatac atgctccctt tataacgggt ggagatcccg
    14401 tcaggcgcta ctcgtttcct ttcgtcctgc cctcataagt ccattcagtg aagcttctcc
    14461 taccgcaatc gcaccatctg cggctctctg tgaaagaaaa tttcctcgcc tactcttctt
    14521 aatcagcggt ttttctttgg ttaagggcaa ttctacaacc ataaaaagta tttgtcaaca
    14581 ctttttttat ttccaatttc gaatttctta tctctcttta tcaagcttta tttgctaagg
    14641 tatgatttag taaatcgtat cttagcaaat ttcaatttaa taccaggatt tcagagcatt
    14701 tttttcacct acaaggtatg ctaatattgg aaatgcatat aataattata gggagattaa
    14761 tgaaatgagg cgatttaata aattagcagc aagaatattt ctaattatgc ttgttctttc
    14821 ttctgtttgt atcttgccat ctgcttcaag tgatttctat gaaagtacgt acaataatgg
    14881 aaattatatt tgcagatcta cactaactac agattatgga agatcaaaaa ttgagtatga
    14941 ggatagtgct attttagtta catcagaatt gacaatcagc tgcagacctg ttaatggttc
    15001 tcaatgtgtg cagcttccta cttgtcgttc aattgcttat tcttatgccg aagttatagg
    15061 aaatgctgac attgatgttg tattctgttc agcaatacat aactatttaa tatgctcaaa
    15121 ccctgtctat tttgttggat tgactccttg aaacaaaaaa acatgagaaa agtaataata
    15181 tttattctga ttgcatggtt tcttgttctg gcatcctgta attctgatta cagtacacct
    15241 cttaatacgg attatacaac agaaacagac atcagcttcc aaggggatat aaatgataaa
    15301 agatacacga gttttcgatt gtctgatgga tgctcttttt cgcagtttgt agaaacatat
    15361 gaagggtatt actatctcaa aaacttctat gtttactact gctcagacgg tatatcaaaa
    15421 tatgtgaagt tatgctcaaa acctgactgc agacacagtg ataataactg caatgcatat
    15481 actggagcaa atttaatcgc atattatgat ggatacatgt attatgtagc aattgatcaa
    15541 tatgactatt gtctgttcag aatgaatatg gacggaacca accatgttaa gataaaatca
    15601 atttctacaa tacaggaaaa gtgtacaggt ttttttgaac atgggtactt gtattactat
    15661 aaagggacga tttatgggtt attgggtaat accaatactg tgatatatag aacagcggtt
    15721 tttgacaaaa gtgaaggaac aatagtagtc gatacaaaag ataacggaat aaatgttgat
    15781 gatattttct tattctaccc acatgatgaa tatatatatt tctatacttt ttcacctgat
    15841 acttattact ctgtgtgtag atattccatg gaaacatcca aatggagtga ttatgtaaat
    15901 actgaaatgt ttttccagag tgtactgaca gatgccgaca aagtttattg gtatctgaat
    15961 ggtgacggtt tttatgaata tgcatatgaa acaggtacaa tagagcgtgt agctgatact
    16021 gtcggtgacg gcttatatgg agtttcatat aacgacgagt acatatatgc atatcaatat
    16081 tacgaagaaa aatattcacc tattccgcca atgctatacg ttttcgacag gcaatacaac
    16141 cttattgaca atgtgtcggt agatattcct atgcttaatt acttttctcc attttatctg
    16201 acagagcttg atgactatgt gcttttgtca acatcgataa acgacagcgc gcctgattat
    16261 tatattaaga agtctgaatt cggaagcggg aatataacgg tgaggaaaat cggtgactga
    16321 ctactcatac aatgtattta atttatatta ttacatctgt atcaagcatg ctgtgtagct
    16381 taatatgaat gaaaatatat tcatgtgagt aaaaaacagt ttgactagcg aaatatactc
    16441 aataattcat ccattcctta ctgataatga acgcatcata aaacataggc aattatattt
    16501 acgaaagata catatgaaat aattaattat gaaccaaaag tgctccatgg aacagaaatt
    16561 gtttgtattg attgaccctt tatttatttt gtgatatggt agttacgtac caaaagtgct
    16621 ccatggaaca ataattgtac ataaggatat gaaatgatag aaattaaaag agataaatat
    16681 ctgaataaat tgataaagaa aaaagataac ggacgggtga agattatcac tggtatccgg
    16741 cgttgcggta aatcctatct tctatttgaa ttatataaga actatttatt aagaaatgga
    16801 atttcagaaa atcagattat cgagatgcca ttggatgaaa tcgataatat acaatatcgt
    16861 aacccgtttg agcttaacgc ctatatcaaa gagagaatta ctgataaaaa taagagatac
    16921 tatatattta ttgatgaaat acagttttgt gaggaggttt caaatcctta tataaaagat
    16981 tctaatgaaa aggtaacatt tatagataca gtacttggtc ttatgaaaat aagtaatgta
    17041 gatatatata ttaccggaag caattcgaaa atgctatcaa aggaagtact gacccaattt
    17101 agagatagag gagatgagat tcatgtaatg cccttatcct atgcagaatt ttgggttgcg
    17161 tatcagaaaa ttcatacaca ggttaaatca cccatatctc caattatgat taatagaaat
    17221 gatgcatggc gggattactg tagtttcgga ggtatgccct atgtcctggt acttgatact
    17281 gttgaggaaa agagtcagta tctaaaggat ttatttgatg aaacatatat aaaagatatc
    17341 attgaacgga ataatattag gaatgataag gaggtcctgg agattttatt ggattttgta
    17401 tcttcttcca tcggatcact tacaaaccca agaaaacttg aaaaccgttt tctgtctgag
    17461 agaaaaatta acatttcaca cagtacgata acaaaatatc ttgacctctt cattgaagct
    17521 tacattatgg atagtgcaaa gcgatatgac attaagggat ctcagtattt tgatactcca
    17581 ttgaaatact atttcactga tatcgggtta cggaatgcgc gattgaactt cagacagatt
    17641 gagcagacgc atatcatgga gaatattatt tacaatgagc tgattaacag aggctacaat
    17701 gtggatgtag gagtagtcca atatgcaaaa cgtgaaaagg ataatcaagg tgaagagaag
    17761 aaagttagag tacagttgga agtggacttt gtagtcaacc atggtaacaa acgttattac
    17821 atacagtcag cacttaatat tgataatccg cagaagagag aaaaggaagt gaattctctt
    17881 aatcgtatag atgattcgtt taagaaaatc attgttgtaa aagatgatat tattccctgg
    17941 tatgatgaaa aaggtatcca atatgtcgga gtacaggaat tcctgctgaa tgaaagagct
    18001 atggatatct gaccggataa tattagaaaa aattatggaa tagtttaata gaggaatttc
    18061 tattgactag atgttacttt gaccatatca tttataatta atgagcatat tagtgttgaa
    18121 tgtataactt tttatatcta tatattacct aattacagag aagagataac ttatgggaaa
    18181 aaggagaggt ctattctctg catcactgaa tattctgatt gcctggacaa taggacagat
    18241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gaggtctatt ctctgcatca
    18361 ctgaatattc tgattgcctg gacaatagga cagatgctgg atatcgctat aggaggtaaa
    18421 tggagtgaag ccattacttt cattattctg atttccgtct ttgtgcttgg cagaagcttt
    18481 attggctata ccaactcgct tacaagctat aattatgagg cacaggcggg atatgttatg
    18541 cgctgttcag ccatggataa aatcaatgct ctgccaatag ggtatttcga gtccaaacat
    18601 actggaactg ttatttcacg tatgatgaac gatattgata agatgcagga gtttctaccg
    18661 aattctgttg ctggtatttg gagccatact ccagtaaata tgatttttgg gctgacgctt
    18721 ctgttcctta ttgactggcg gctgacattg attgtggtca gtatcgtaat gataacgact
    18781 tttatactga gcagaatttc tgtacctcta gttaagttta ctgatgcaga acaggaaagc
    18841 aaagttaaat acaatgctta tcttcgtgat ttcatagaag gaaatgacat ttataaaata
    18901 tatggaatga aaaggactca cggaaaaaag ttcgagtccg ctgttgacga acagtttgaa
    18961 tgcagtaaaa agatcaaccg tcagaaaagc aagatgaatg ctattactgg aataaactat
    19021 tatctgccat gggtcctgtc ttatgttatt ggcagttttt ttgttgccaa aggggatatt
    19081 actatcggtg ctttatactc ttttgcagca attcttccca atgtaactaa agttgcatgg
    19141 cagataggta acaatttagc tgctatggtt gagacaagcg gaatagccaa acactatttt
    19201 gaacttatag atcagcctga tgaacgaaag aacggacaag atttcagttc agtaaacaat
    19261 aatacgatag agtttgattc tgtaagttat tcttattctt ccggattaag catcctctct
    19321 ggatgctctt ttatagtttc ggcagcttct acggttgcgt tagtaggggg cagcggaagc
    19381 gggaaaacga caatattcaa attaatttgc ggatattacg aggaatacca tggagatgta
    19441 aaaatatgcg ggtatccgtt aagagaatgg aatctgcatg ctttacgtag cagtattaca
    19501 tatatgagtc aggagagtta tctttttgat gatacaataa tggagaatat aaggaatggc
    19561 cgtcctgaag ctacggataa tgaggtcatt gacgctgcga aagcagcata tgctgacgaa
    19621 ttcataatgc agacagtgaa cggatataac acggttttag gtgagcgggg tataatgctt
    19681 tcaggcgggc agcgacagcg tattgctatt gcaagagcca tgttgcgaaa cacgcctata
    19741 atactgcttg atgaaccgac ttcttcattg gatacccgtt ctgaatacca tgtccagctg
    19801 gcactgcaga atctgacaaa ggacaggact gttttcgtca tagcgcatcg tctttccact
    19861 attgaaaagg cagatactat acttgtactg gagaacggaa gaattattga gcagggaccg
    19921 catttagaac ttgttaagcg aaatggccgc tacaaagaac tgtattcagc ccaaattgca
    19981 gaggtgaatg caaatgaaac gcaatttctt caaggatttc atgcgatttt ctaaactttg
    20041 cggcagtaag agcagaagac ttcttgtaat aggtatctta gatggattag taaatggggc
    20101 attgggaacg atttttcagg ctcaggcgct tagatatata attgcgtatt tcattaaaaa
    20161 gcaatccgat gctttaagta aggcatttgt tatgattggt tttacaatgc ttgtaggttt
    20221 tgtatttcgt ccgcttataa attactacag tcaggtcact atcagtaaga ttacggcttt
    20281 gttacgtatg cgtctgtata agcatcttac acaactgccc acatcatatt ttactggtac
    20341 tcacagcggt gatattgcag cccgtatcaa cggagatgtc gaagcgtttg catgggcctg
    20401 caatacttta ctgaactttt taggtgcagt tattcctgct ttcgtgttaa tgccgtatct
    20461 gctgacactg gatatgcgga tggcactgat tattggagta gtcggactgt tgtttttgat
    20521 acttacagct aaggttattg aacctcttag aaagcgacaa aggaagatac gcactgagat
    20581 ttcaaatatg tcagtttctg ctgctgatgg tattatgggt tttcaggtga taaaaagtaa
    20641 taatcttgag gaacagtact ctaataaatt caagtctcat gcggaaaaac ttttggatac
    20701 tcaaaaggat ttcgggaaag taaatgcaat tgtgggattt ttcaatatat tcatatggtg
    20761 tgcaggagag gcatttattg caatattcgg tttggtattc gtattaaaag gcaccctcgc
    20821 tgtaacgaat cttgccgctt tggtatcagc aggccggagt ataataggta tctttactga
    20881 tttttcatct ttgccaatac agttgcagca ggcttttgcc ggtgttgaca gagtatatga
    20941 actttgtgat atattaccag aggctgagcg atatctgatt aacggcaatc gatcagaatg
    21001 cggaatctgt gttaagaacc tgagtttttc atataacaat aaaactttag tactggataa
    21061 tatcagtata tatgctcaaa agggtgaaag tatagcatta gttggtgata gcggaagcgg
    21121 gaagacgaca cttattaaaa ttcttgcagg tttatacagg attgatgatg gggaaatcac
    21181 agtgctaaac cgtccactta gtgattacac gcttgatgaa taccgtgcaa aaatagcata
    21241 tgtcccgcag aatgcatata tctttgatgg gagtatagcg gaaaatatat gttatggcag
    21301 agaaggagca ttattagacg aggttaagct tgcagcacga aaagctaatg ccgaattatt
    21361 cattatgcaa aaacctgcac agtatgaaac ccaggtcgga gaacgcggaa tccagctttc
    21421 tggcggagaa cgtcaaagaa ttgctattgc tcgcgctata cttaagaatt ccgagattct
    21481 gttactggat gaggcaactt caagcctgga tagcgaatcg gaacttgcaa ttcaggatac
    21541 attggacaac ctcattcaac agcacaccag tgtaattgta gcacaccgtc taagtactat
    21601 tgttaatgct gattgcatct atatgatgca aaaaggcaga ataatcggtt ctggaaaaca
    21661 tgaacagtta cttacctctt gttctgaata caaacaactg tattacagat tatttgcatc
    21721 aaagaaagac caggaataaa tgtaaatgca ctccttcaaa ttactttttt atacaagctg
    21781 gtaacaaact attaataact aagctttaca tttttaaatt tgatctatag ggttatactg
    21841 tagtcaaggt taacctgtaa aacaaaagga gaacatttat gaaaaattat gaacttataa
    21901 ctaaatgggt tattgacacg gtaaagaaag actattttga tgatattgct ttagttgtgt
    21961 cacacactac attacgcatt gatgaaagtg aacaaactat atcatatttc gtaccaatta
    22021 ccgaaaaagg tcgtaaattc ggacagacat ttatattgca aggtgaaggt tttgacattt
    22081 ggggaattga ttggaaacga cttgaaaaat ttgcagagct tgatgaatat aatatcactg
    22141 ttcttgcaga cagcaaagta ctatactcaa gaactgctga agatgaacaa cattttgctg
    22201 cattgaaaga aaagcaatta tcaaatttgt cggatatgag aaaaatgcgt gcttgtgccc
    22261 ttgcggcata tgcgcaagca agaaagattt attttgaaat gctttttgca caaacagaaa
    22321 gtgacataaa atttgctgcg ggatatacaa tagattatct tgcacaagca gttgctttta
    22381 ccaatcagaa gtattttgct tactctcaaa taaatcagct taacgagctt aaaaaaatgg
    22441 aaaatgtacc tgctagattc agtgagttat acaatagtat aatttacgaa gctgatgttg
    22501 aaaaacaaaa atcattatgt ctagaattaa taaatgtagt tcgcatattt ctagaagata
    22561 ataagatgaa caatgatgaa aaacgatctt gtacagaaaa taattatcag gatctggctg
    22621 attggtacac ggaattatca tatacctggc tcagaatacg caaatatgca gaattgaatg
    22681 atgtaactaa aacatatatg tggggaataa tgcttcaagc agaattaaat cgtgtatgtg
    22741 atgattttgg tattgataaa tatgaattaa tgtctaattt taactcagag aatctctctg
    22801 gttttgcagc tgaatccaat gctattgaat gtaatattag aaagttaatt acagaaaacg
    22861 gaggaatcat cagggaatat aatagttttg aggactttgt aaatgaagta taagaatgca
    22921 caggaaatcc taccagataa actgctcaaa gagttacaga aatatatctc tggaggagca
    22981 atatatgtgc caaatataga gtctaaaaaa caatggggag agcaatcggg agcgcgatgt
    23041 tactacaaac aacgcaacga agaaattcga gaaaaccata aaaacggaaa atcactagat
    23101 gaactttctt tcgaatatgg tttgtcaatc gaatctataa gaaaaatcgt ttactccacg
    23161 aaatgaatag tttattcagt acttaaagga ataaaagtaa tagtaagaaa gaataacttc
    23221 ttatcataac taatcagcac ccttttgatt aatcattgaa gggtgctgtt cttaattcta
    23281 accttatgtt tatagctact aaaagacatt agcatattac ggtgctatct gctttatcaa
    23341 ttcatctgtt atttgttttc ctaaaaggta atacttcctg tcaggatacc agaacattaa
    23401 tttgttcttg aaataggtca tgcttgtata tgtgccggtt tcagatgtgt tcttaggtcc
    23461 gaccgctatg tcttctgtat ccaaaagttt atacttcatt ccaaaacgta taggctgttc
    23521 tgctttggca tcaaaacggc cgatggctat gtataaaggg ttacggatga aattagacat
    23581 gttgtattcc cagttttctt ttttctggga aaagcctata cgctctccgt cattatcatg
    23641 aaaggctgtc aggtagagac cgtctttaat ggagtatatt ggagcaggtg atagaggatg
    23701 ctttacatac tctaggttgt caccatatct taaaggctgg gcttttttga atgtatggcc
    23761 gttatcttca gatgtgctat agtatacata ccctgtcact gtcctcatga tacagaatat
    23821 acgcccgtca ggaagaagag ttattgcagg ctcctgagct gttgacatat tcggtataat
    23881 cggattatca acctcaatgc ctttgtcatc atcaggaagc catgttattt ctatatcata
    23941 agggtcagga ttgtcatcta tgttttcaaa tctcatgaaa tagcagcggc tatcctgatt
    24001 gacccaccgg gtcttatcat aatcagatat attgtggaca tggcttgtgc ataaggtata
    24061 tccggctatg tatttattgt ttgcatcttt aataggtttc tgccagacta tccagttttt
    24121 cgggtatttt gtatccgggt tgtcaaagcg ggatcttgcc attggaagaa cagcttcatc
    24181 tgaccaggtg tatccataat catctgaata gatacaaccc aaaccaccgc ttcctgcatg
    24241 atgattgtca gacagttcaa gctgcttagt atagaaaagg tatatccttt ttttagaggt
    24301 aactatagga aaagcccagc ttgcctgatt gaatctttca ttttccttag taccagctat
    24361 atatacagga gatgtccagt taacagaatc tttgcttctt gacattacta tatgattgtt
    24421 accttttcct tcacaggaac tttgtgtcca gacagctaaa agctccaact gatctggaga
    24481 ctcaaagact aatagatgct cgttatcacc tttataatat tcaataccgt caggattata
    24541 cagtataaca tcaggactgg ttcttttata ctcaatcagc gggtcacctt ttggtatgcc
    24601 ttcaaaggct ttgccataag gatagttatt ctcaataagc cattcacgtg aatacagttt
    24661 catctctcct ccaaaatatg cagtgtcagt aatagtatac attatgactg cttgaaatga
    24721 gagaatttaa ttaaattaca atacataaac ctataatgaa gtttagggaa aatagtaagt
    24781 tttgcgatta taacccgaaa aactttctgg attaataact ttttaccaaa atatcttata
    24841 gaatttactt tccttcctga atttccgttt ctgtgaattc gtgttatcaa tgtccctcca
    24901 ttcttttaga ttgaattgtg tcgacactta tgcttatgaa tataaaaaca gcataacctt
    24961 tatcgatgca agttatgctg ttattgttta ggaatataat ctttatgcga gaagtatttg
    25021 tgcttctcct attatttatt aactcagctc aaactgccct gtataaagct tgaagtatct
    25081 gccttcctgc tttagaagat tctcatggtc tcctctttcg attatctggc cattttccag
    25141 taccattatc gcattggagt ttctgacagt agacaatctg tgtgctataa cgaatactgt
    25201 tcttccttcc ataagcttat ccattccttt ttcaatcaaa gcttcggtcc tggtatcgat
    25261 ggagcttgtt gcttcatcaa gtatcagaac aggtgggttt gcaactgcag ctcttgctat
    25321 tgaaagaagc tgacgctgtc cctggctcaa gtttgctcca tctgaagtta ataaagtgtc
    25381 ataaccttca ggtaggtatt ttatgaagga atctgcgttt gcaagctttg ctgcttcttc
    25441 aacttctatg tctgttgcat ccagtctgcc ataacgtatg ttttcacgta ttgtacctgt
    25501 aaacaggtga gtgtcctgca ataccattgc aagagatctt ctcaagtctt ctttcttaat
    25561 cttctttata tctataccat cataagttat ttcgccctgc tgtatttcat agaagcggtt
    25621 tatgagattg gttatggtgg tttttcctgc gcctgttgaa cctacaaatg ctatcttctg
    25681 gcctggtttt gcatacaaag taacattctt tagtacatcg gttccttcat tgtatgcgaa
    25741 agtgacgtta ttaaaccgga catcgccttt taattcaata tattcaactg tattatcatt
    25801 gttaatatgt ttccatgccc aatgacctgt ctgtgtggag cattcagtca tatcattccc
    25861 atctttgcat acatttacaa gtgttacata accttcatcg aattctttgg gttggtcgag
    25921 tacttcgaat attctttcag cacctgcaaa agcagctaag atagcattta tctgctggga
    25981 tatctgagtt ataggctgtg agaactgtct tgtgaattgt aaaaaggaag ctaatgagcc
    26041 tatatccagc attccgttta tacacaatat cgcaccgaca gctgcagttg cagcatagtt
    26101 cagatgtcct aaatttccca tgataggcat tataatattc gcatatgtgt tagcatcggt
    26161 agctgcaatt ctcagattct cgtttttctt gttgaagtcc tctacaatat tatcttcata
    26221 gcagaatact ttaactactt tctggccttc aatcatttcc tcaacaaaac cattaacctc
    26281 acctagttct ttttgttgtt ttttaaagaa cttggcgctg ttgcttccta ttttctttat
    26341 gataaggaac atgacagcaa gagttattac tattattaaa gtaagtatcg ggcttaagac
    26401 aagcatcaga gtgaaaatac cgacaactga aacagatgaa gatatgaact gaacgaaacc
    26461 attgcttatg gcctgacgaa aggtttccac atcatttgta aatcttgaca taacctcgcc
    26521 atgcgtatgg gtatcgaagt atttaatggg aagatcctgt attttgttaa acaggtctct
    26581 tcttaaattt ttcagtactg agttggttac cttcatcatc tgatagttat acagataaga
    26641 ggctattacg cttgaaagat atataccagc cataaccata aggaacttga ttaaaggaag
    26701 ataatcctca cctgtgggat ttgtgcctgt caatggagct atatagtcat taataagagg
    26761 ctttaaaagg tatgaagccg ctactgtgcc agctgcgctt aataggacta atattatgac
    26821 aaatatcagt cttgtcctgt atgcgaaaag ataatcaagc agccttttaa aagtcttttt
    26881 tgtattcttg ggtttctgga acctgtcaac ctgagggaca tgtcttttct gttgctgtgt
    26941 catatcaagc cacccccttt tgttgagaat agtaaacttc agaataaata gtatttgttt
    27001 ttaatagttc ttcatgagtc ccgactgcat caattttacc gtcattcata actataatct
    27061 tatcagcgtc atatactgag gttatcctct gagcgattat gaggacggta gtatctgaag
    27121 tgttcttctt aagctcatta cggatggatg cgtctgtggc ggtatctact gctgatgtag
    27181 aatcatccat gataagtatt ttcggattct taagcaatgc tcttgcaata cacagtctct
    27241 gtttctgtcc gcctgaaaca tttactccgc cttgtcctaa attagtcata tacccgtcag
    27301 gaaaactcat tataaagtca tgagcatttg caattttaca tgccttttct atttgttctg
    27361 tcgatgcatc aggatttccc catttaagat tatcaaggat agttccggaa aatagcgtat
    27421 tcttctgaag gacaacgctt actgcatcac gtaatgtttt aagtgagtag tcttttacat
    27481 ttcttccacc gacaagaacc tcacccgtat cagcatcata aagccttgga atcaactgga
    27541 taagagttgt tttagcagaa cctgtgctac caatgattcc tactgtttca cctttgttga
    27601 ttataaggtt aatgtcttca agtatataat tttcattctt ctttgaatac ttgaaaaata
    27661 catttttaaa ttcaatatca cctgattcta cagataattc cttgtcggaa tccttatcag
    27721 taaggtctga aactgtatca agaacctcag caacacggac tctggatgcc cgtgacatta
    27781 taagcattat aaatataaaa gagagcatca taagtgacat caggatttgt cctatatatg
    27841 atatgaagga tataagctcg cctgtaagca tctggccaat tattatctgc tttccgccaa
    27901 gccatagtat tgctatcata cagctgtaca ttgcaagcat catcaacggc attgttgtta
    27961 tgagaagttt ttccgcatga agggaagcag ccatattctc attattagtt ttattgaatt
    28021 tttccttttc gtggcctgaa cgaacaaatg cttttacctc acgaatagca attaaatctt
    28081 cctgtacaga tgagttaata gtatcgtact ttttcatcat ctcctggaaa cgggggaaag
    28141 cttttgtcga tatgattatt atcacgccta caagtattgg aataacaact aagaataccg
    28201 acaccaatga cttatttatc cttatagcca tgataagagc gcttatgaac atgaaaggcg
    28261 atctgactgc cattctaagt accatattga aagcgttctg tatctgggta atatcctgag
    28321 tagagcggga tatgaggctt gctgtagtga actggtccat atttgaaaaa gagaagtctt
    28381 gtatctttga gaatacgcct cttctaagtt cagcaccaaa tccgattcct gcgacagctc
    28441 ctaatcttga agctatcatt ccacagccta agcttaataa agccattaat accatcagta
    28501 atccctgatt tactatatac cctatatcgc tattggcaat tccgacatcc actatctttg
    28561 ccatgataaa tggtatgttt acttccagca agacttctgc aattatgaac agcggcgtca
    28621 gaaatgtctg aagccagtac ttctttgcat atggaagaag tttacttatc atcctaatcc
    28681 tcccttttta tggatctttt tctcttcaga tatgagttca tgtatatttt ttgttttaaa
    28741 ttcttctgta tttaatgaat ccagaatttt gtttatgagt ctttcaaatt ctaaaagctc
    28801 attatcacta aagtccctaa aagccatttc atctatggct tcgcattctt ttttcatagc
    28861 agttgatatg tcatgggctt ttttggtcgc atggagtctg ttgaccctgg aatcagactc
    28921 atcagttaat ctattgatat atccggattt cataagcctt tttactgatg ttgcaatact
    28981 tggagctgaa accttgaaaa aactgaccag gtctttttgt gtgcacccgt cattgctcat
    29041 gatatagtgg attatgggaa gctggccaag atagatatcc ttattctcag ctgctttatt
    29101 gaaatacagt ttatgcagca atgttatttt tcccagcttc tgtaacgtag tcttgtgttc
    29161 catttgaccg ccttaataaa ttagtcaacc ttaatactta atcaggttaa ctaatttgaa
    29221 atgatactat ttctattatg gcttgtcaac agttttcaca aatagttcaa aatagtttta
    29281 tatttatgag acatagagaa acatcattca aatatcatta agatcagaat gtgaaatgta
    29341 ttaagagatt gtattaatat aatttctatt tattatatat ttcgcttcgg tgccctatgt
    29401 gcaggataag tataagaact gtgttatcct gtatttctgc tatcagtcta taattgccga
    29461 tacggtagcg ccactgtccg ctaagattag ctttaagctc tttaccgaat tgtctgggat
    29521 ttgaacagcc ttcgatattt ttttgtatcc accccataat cattgcagca gtatagtggt
    29581 ctaatttttt cagttctctt ctggctcttt cagaaaacac gacatggtaa ttcaacgtaa
    29641 tcccagctcc ttttccattt cttccatagt gtaggtagta ggatttgtgc ggtattcagt
    29701 catagcagtt tcataaactt tcaggtcaaa ctcttcctca atacgttcca gaacagtctg
    29761 acgtattaat tctgagacac ttatgccttt catttctgca tattttctga ataacatagt
    29821 atctgcttca tttaatctta atgatatagt catatcagac ctccttttgc gtattacatt
    29881 gtattactta tttattcaga tgtcaattaa tttactatat ttgcatatac aa
//
LOCUS       DAOSXM010000019        29462 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2111
            NODE_1009_length_29462_cov_12.049954, whole genome shotgun
            sequence.
ACCESSION   DAOSXM010000019 DAOSXM010000000
VERSION     DAOSXM010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864178
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 29462)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29462)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:24:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,261
            CDSs (total)                      :: 2,229
            Genes (coding)                    :: 2,218
            CDSs (with protein)               :: 2,218
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 3 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29462
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1009_length_29462_cov_12.049954"
                     /isolate="AMR_MDS_2111"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     regulatory      36..136
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            193..675
                     /gene="purE"
                     /locus_tag="PLQ68_04285"
     CDS             193..675
                     /gene="purE"
                     /locus_tag="PLQ68_04285"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013656490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HXK71904.1"
                     /translation="MKVLVLMGSDSDYKTVEPGLKFFEDLNIEYQVRVCSAHRTPHIA
                     AELASNAKSNGFKVIIAAAGKSAHLAGVLAAHTILPVIGIPVKTSDLGGMDSLFSTVQ
                     MPSGVPVATVAIDGGKNAAILAVQILALSNEELSLKLEQFKKDMEQQVIQKDMEINKK
                     "
     gene            677..2044
                     /gene="purF"
                     /locus_tag="PLQ68_04290"
     CDS             677..2044
                     /gene="purF"
                     /locus_tag="PLQ68_04290"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006877192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HXK71905.1"
                     /translation="MSGVFGVYSKDKSVNIADTIYYGLYALQHRGQQSAGIAVRDNEL
                     IEWYKAKGLVPEVFKPTDLEKLPGDAGIGHVRYSTSGDVSKGTIEPLVLKYISGYMAI
                     AKNGNIINSNELREELEANGCMFHTTNDTEVIAALLTKFRIGSEHIEDSVEQMVKYIK
                     GSYAITMITPRYLIGLRDPLGIMPLCIGKINDSTYIISSESAGIDAVGGHFIRDVEPG
                     ELVLIDSNGIQSRKLFECKKSSLCIFEHVYFARPDSIIDGASTHDARVNAGRILAKEQ
                     PVEADVVIGVPDGGTDMAFGFSQGSGIPVAAGLLRNKYVGRTFIAPNQNDREIAVKIK
                     FNPVKTEVKGKRVVLVDDSIVRGTTTKILVRMLREAGAKEVHLRICSPPYKFPCYFGV
                     DTSSRGQLLASNNDKEAICKWIGADSLEYISYEGILKAPINSRVGFCTSCFTGIYPID
                     VPKEV"
     gene            2072..3124
                     /gene="purM"
                     /locus_tag="PLQ68_04295"
     CDS             2072..3124
                     /gene="purM"
                     /locus_tag="PLQ68_04295"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004803473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HXK71906.1"
                     /translation="MKDAYKEAGVDVRAGYESVALIKKHIQRTVIPGVLGGIGSFSGL
                     FEMDLKKYPNPVLVSGTDGVGTKLKLAHSLKVHDTIGIDAVAMCVNDVLCQGADPLFF
                     LDYIACGKNFPNIIEQIVKGVAEGCIQSECALVGGETAEMPGFYKEDEYDIAGFCVGV
                     VNKPDIIDGSAIEEGDVLLGLASTGVHSNGFSLVRKIFGDDAQSLNMTYPGFYKSIGD
                     ILLKPTKIYVKDIKRIRNIAKIKGMSHITGGGFVENIPRMFSQREDLTPVISKGTWDI
                     PSIFDLLAEKGGITEDNMYRTFNMGIGMVIALSKEDARKVKNELKDDIQEIGYVAAAK
                     EVKDKKNIEENIYIRG"
     gene            3130..>3512
                     /gene="purH"
                     /locus_tag="PLQ68_04300"
     CDS             3130..>3512
                     /gene="purH"
                     /locus_tag="PLQ68_04300"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256141.1"
                     /note="involved in de novo purine biosynthesis; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HXK71907.1"
                     /translation="MIKRALISVSDKSGIVEFAKKLSDMKVEIISTGGTSKILNENGI
                     KVIPIENITGFKECLDGRVKTLHPNIHAGLLARRDLKDHMDTLDSLGINPIDMLVINL
                     YPFKKTIMKSDVTKEEAIENIDIGGP"
     assembly_gap    3513..3612
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3613..4530
                     /gene="purH"
                     /locus_tag="PLQ68_04305"
     CDS             <3613..4530
                     /gene="purH"
                     /locus_tag="PLQ68_04305"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HXK71908.1"
                     /translation="IQEMRYGENPHQKASFYKEPRRTKGTICDAVQLHGKELSYNNIN
                     DADGALSMLKEFPNEVACVAVKHANPCGVGIGKNVFEAFKKAYDSDPVSIFGGIVAFN
                     RTLDVVTAEKLHEMFLEIVIAPDYKDEALSLLKKKKNLRILRLDDITYSDEFEYVMKK
                     VSGGMLVQSKDNTVVNMDDLKVVTKKQPTEQQIKDAIFGMAVCKHVKSNAIVLVKDMA
                     SIGIGPGQMSRIGAANIAISLAGERAKGSVMASDAYFPFDDCVIEAKKAGVECIIHPG
                     GSISDKDVIAKADELGISIIFTGMRHFLH"
     gene            4544..5800
                     /gene="purD"
                     /locus_tag="PLQ68_04310"
     CDS             4544..5800
                     /gene="purD"
                     /locus_tag="PLQ68_04310"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020815441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HXK71909.1"
                     /translation="MKVLVIGGGGREHAVVKKLSESPKINKIYCAPGNAGISQIAQCV
                     NIKATDIDGILKFVDENPMDMVIVTPDDPLALGLVDKLNEKKIRAFGPVAKAAELESS
                     KAFSKNLMKKYKIPTGDFEIFDNAENALKYLKDKKYPLVIKASGLALGKGVIIVSDQE
                     QAEKTIDQMMRNDMFGTSGHTVIIEEFLQGPEVTVLAFTDGNTIKTMPSSQDHKRALD
                     DDKGLNTGGMGAFSPSRHYTKDIEDYCINNIFYPTIKAMKKEDRNFKGVIYFQMMLTK
                     DGPKVIEYNARFGDPETQALLPLLKTDLYDIFNAIIDETLDKIDIIWENKASATIVLA
                     SGGYPKEYIKGFKITGIDEVQNNGITVYHMGTAIKDGNIVTNGGRVLCLTATADNVED
                     AVAKVYNNIEKINFKDMHFRRDIGKK"
     gene            5797..6396
                     /gene="nadD"
                     /locus_tag="PLQ68_04315"
     CDS             5797..6396
                     /gene="nadD"
                     /locus_tag="PLQ68_04315"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:NF013621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HXK71910.1"
                     /translation="MKLGILGATFDPVHKAHLQMAKYALDYCEYVLLTPCTKPPHKDH
                     DSITEDSIRYMLLCLALDGYERLIASDIELKRKTTTYTIDTLRQVKEEYPAFDEIVYI
                     IGADTLFQLTTWKEHEEVFKLCSFLVFEREGFTRKQIHERIDILKKDYNANIIFDIRS
                     VSEISSSVIRKAIVEGDDSIEWMLDKRVYRYIKENGIYK"
     gene            6380..6955
                     /gene="yqeK"
                     /locus_tag="PLQ68_04320"
     CDS             6380..6955
                     /gene="yqeK"
                     /locus_tag="PLQ68_04320"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: protein motif:HMM:TIGR00488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HXK71911.1"
                     /translation="MGYISEKAKKDIISRYSEERYEHIQNVIELAVKLAEKYNADVEK
                     AYTAALLHDIAKDMEVDEMVALLESADIFIDEEYHNKNILHGPCAAVIAYENYDIDDE
                     EILYAIYYHTFGRAGMGLMEKIIFISDAAEKGRTYKNVGKIRKAMNTDLNMAIEMSLS
                     GTISHVLSKGEVLFPATAAALEYFKEQRIKR"
     gene            6958..7914
                     /locus_tag="PLQ68_04325"
     CDS             6958..7914
                     /locus_tag="PLQ68_04325"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HXK71912.1"
                     /translation="MYKCIFKNAYKIIKNDESYLAIRFTEKQLEYYKNKSAAAEDRSR
                     DTAGICMDFYTDAEEISFAYKACCFSRRYVGFDFYEDGIFRKHIEEALDTKQSVITHP
                     LEKKGYKRVTVYIPNLSEITMYDLNIGNFKPIEENSEKKLLFLGDSITQGMVAQSPSL
                     AFTTQLARAWKAESLNRGVGGARFEADSLDDEDEYNPTHIFTAYGINDIYHLECVDEL
                     PAVLNQADIYLQKLKKIYNDALVYVITPIWCLRYDEDKYFAKIFDEYTGKLMKIVEKY
                     GFTVVNGMNLVPHDTKYFQDHSVHPADSGFNHYAENLIKAIE"
     gene            7998..8762
                     /locus_tag="PLQ68_04330"
     CDS             7998..8762
                     /locus_tag="PLQ68_04330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK71913.1"
                     /translation="MGLQNNAKKWVRTSIIIMLIGIAMIIAGITIDDYNFYWMIYVGA
                     ILFLTFLIAFFMFVNQANRLDRLFRGEELLANWKFEENERLAKTEEELIQRKAQNKVM
                     LLIIAGFFVVIGALFVIFGFDDFEDALLFILIMVGVLAFISVVALITPGSRYRKMKNA
                     AAEVFVGPYSVWVMGEYVQWKAPLTKITSIIYSKDREDNYTITINYFIWQRYGPQPHT
                     CRVPVPLGHETEAESVASDLASINNVKYINKLIDAE"
     gene            complement(8765..9193)
                     /locus_tag="PLQ68_04335"
     CDS             complement(8765..9193)
                     /locus_tag="PLQ68_04335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK71914.1"
                     /translation="MKTVIVYYSYGGKTKKYSNALAKETQADVYEVDTLKKRSFIGNL
                     FLECPKALTQKPTTIKDINLDWSSYEKVILAFPMWAGFPAPTFNNIVNILPSGKEVEI
                     IIISGGGITKPENKEKIIALVNKTGSTVVNYKDVSSTSVK"
     gene            complement(9309..9884)
                     /locus_tag="PLQ68_04340"
     CDS             complement(9309..9884)
                     /locus_tag="PLQ68_04340"
                     /inference="COORDINATES: protein motif:HMM:NF025736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HXK71915.1"
                     /translation="MLAYWGTQFLVKKPHMLSSKIDDKIPFIPVFIFPYVSWYFVMIS
                     LPFILFYNSIQTYALYIMSLLMVLVISGIVYLVYPTTFYRPEPKGAGFSVKVVKLIYA
                     NDKKILSCMPSLHCSLSMLFILAAATADGLSLVFKIILIVLSFLIILSTVLVKQHVLI
                     DVITALPLSVICWYIAMAIGADAFLSFFNFT"
     gene            10128..10508
                     /gene="rsfS"
                     /locus_tag="PLQ68_04345"
     CDS             10128..10508
                     /gene="rsfS"
                     /locus_tag="PLQ68_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015924868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HXK71916.1"
                     /translation="MDNFDTEKIKNEIVKVLEDKKAKDLISIDVKDVTTLTDYFIICT
                     GTSDRHRKSLADDVTEKAEELGLEVSAPIEGYDKAKWILLDCKDIVVNIFDRETREKY
                     NLEKLWSDGVLEEIRANSNKEEKK"
     gene            10505..>11046
                     /locus_tag="PLQ68_04350"
     CDS             10505..>11046
                     /locus_tag="PLQ68_04350"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HXK71917.1"
                     /translation="MIITGISDEAGKDIATQIKAHNKLGWTALELRNHNGVNPSTTLF
                     TDENFDYIRKVIEENQITVTGFSSAIGNWSRSIRGDFNIDKEDLKVAAKRMKMLNVKY
                     MRIMSWIPDEDDPSYTKKEVIRRSRELAKMAEDLDVILAHENCTGWGGESPENMLELK
                     SAVDSPNYVLLYDTGNVTAHG"
     assembly_gap    11047..11146
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <11147..11671
                     /locus_tag="PLQ68_04355"
     CDS             <11147..11671
                     /locus_tag="PLQ68_04355"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HXK71918.1"
                     /translation="PIPGQVTYFVGGMLFGTWGGFLINHTATILASVTVYWLGRAFGE
                     PLVDKLAGKGKTKKAVDFVKRNGPIFFLLAFVIPGLPDDLLCYVAGIVTLPFGIFLFL
                     VAIARIPSTLSSSLLGNGVLTWTTTQTILVVAILAVFIALVVVFRKPITNLLNKLVRT
                     KSKDENVDITDMME"
     gene            11721..11797
                     /locus_tag="PLQ68_04360"
     tRNA            11721..11797
                     /locus_tag="PLQ68_04360"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11755..11757,aa:Pro,seq:cgg)
     gene            11941..13143
                     /locus_tag="PLQ68_04365"
     CDS             11941..13143
                     /locus_tag="PLQ68_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009620753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXK71919.1"
                     /translation="MNKLIERKAYLEQLSMWREEEMIKVVTGVRRCGKSTLFDLYIEK
                     LKAEGIKEEQIIFINLEDQDFSELLDYKKLHDYVITHMQKDKWTYVFIDEIQNCIDYE
                     RAISSLFLRKSLDIYITGSNAYMLSGELATKLTARYIEIDMLPLSFSEYSEMVDIYDK
                     RKRFNLYMNLGAFPYPARFTDNTLAHNQYLEGIYNTVLVKDVLTRKKLNDVTLVKSIA
                     TFLCDSIGSPVSAKKIADTLTSSGRPTGVATVELYLEALTDSYLFYKVQRYDIKGKMH
                     LKSESKYYICDTGLRNMILGVTNRGIGHQIENIVFLELLRRGYSINIGKAGRGTEVDF
                     VAVRENKTEYYQVAASVLDENTLARELHSLKQVKDNYPKFIITLDDYTGDYDGIRQIN
                     LIDWLDTQ"
     gene            13523..13651
                     /locus_tag="PLQ68_04370"
     CDS             13523..13651
                     /locus_tag="PLQ68_04370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK71920.1"
                     /translation="MKKQDRTEQLFTDMKSVFMPRKNLSALGEKGKWRIVEYKAMK"
     gene            complement(13829..15208)
                     /locus_tag="PLQ68_04375"
     CDS             complement(13829..15208)
                     /locus_tag="PLQ68_04375"
                     /inference="COORDINATES: protein motif:HMM:NF027787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5050 domain-containing protein"
                     /protein_id="HXK71921.1"
                     /translation="MKRRLFLSILLIAIILLSGCNFNRTPSESPSVSDATIESPSSEP
                     SLDPIVTEIIPSEEPVLTVDDLYTKFHEAEASEDYILALGYANQIMSEYPEEQRIYWL
                     KTQMLISCMVAFNNELRKTVESYSTDNSDHKDFIITFKDEYSKTDLSVEWPFIHDYAD
                     GQINIVGSSGTELSSGMGINDYREFRREVFCVQGDWVYYINAEEGYSLYKMKTDFTSR
                     TLLTENEVSNLNVIGDWIYFTDLSKDEHIYKMRTDGSMQTFISDAKCSFMVVYNDYIY
                     YADADSYNNLCRMKADGTGIKNLGHNVEVISISDNLVYFRTEQHELLCMTVEGTDIKK
                     VVEDESYGNFYINEDSIHYMTIEGNGLTIYKCDKLGQNKQQLYHVDYKINFYYVMDTH
                     ILMSVRYPDRMECLVLVDLNDTANVTQLKDYVSNNVIHLKDQNKILFMNDYTNFSWLI
                     LNLADNTVK"
     gene            15447..16574
                     /locus_tag="PLQ68_04380"
     CDS             15447..16574
                     /locus_tag="PLQ68_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003658676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HXK71922.1"
                     /translation="MKKTDTPNIHRYNPPKWFRDADFGIFIHWGASSIPAFAPVDVDD
                     YGTLMKEKPMSYVFANMPYAEWYPNSMKFPGSPAQLYHEKNFKDKTYDYFVEEFKKHA
                     KNVDVEKWAELFEEAGAKYVVVVSKHHDGFVMFDTKIKHPKKEGWHLDFDFVGDLAAA
                     VRKRGMKFGVYYSSLIDWSFKDRRIESAGSLFMGNDNSKLYCDYCYNHWMEIIDRYKP
                     EVLWSDIGYPTDKRLEDLFKYYYSKVPEGVVNDRWGQWPNYLRNKLGEALINRMAQSA
                     LEKNTEVVLDTKYYDFRTLEYNTKWEQKDIWFEVCRGMDKSFGFNQFSREQDYITAEE
                     VKKIISEVFPKKGRLLLNAGPDSYGVIPEYQAEILKKVGNR"
     gene            16574..17092
                     /locus_tag="PLQ68_04385"
     CDS             16574..17092
                     /locus_tag="PLQ68_04385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK71923.1"
                     /translation="MNDKHKKYIVEQMIWSFFINGIINGVIAYFLNKNKQYEILTMQQ
                     NHLDLIIDIAITAIILAWLIAWSVNAGVKKAGLYASLEPRTKFQLWMGKWFKMPATYG
                     WIWCIAVIPVLYGLSALGILIFSITKFTLWGWVIYKTVYTAFLGAGFSSLFITSAYYA
                     DAANLEPKKKKG"
     gene            17098..17421
                     /locus_tag="PLQ68_04390"
     CDS             17098..17421
                     /locus_tag="PLQ68_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013896944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HXK71924.1"
                     /translation="MLRMGSVIQVKTDKLEEYKKLHADAWPGVLDMIRKCNIRNYSIF
                     YRDGLLFSYFEYIGNDYDSDMEKMGADPLTQKWWALTDPCQMPVSSAKEGEWWASMEE
                     VFHTD"
     gene            17449..18687
                     /locus_tag="PLQ68_04395"
     CDS             17449..18687
                     /locus_tag="PLQ68_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009005757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HXK71925.1"
                     /translation="MNSRERILTSINHKEPDHVPLDLGSTPSSNISAIAYNNLKKHLG
                     INTGHTRIYDVVQQLAQPEDDILQRFGVDVLDIGRAFNTLDTDWYDIKLLNGSIAQYP
                     SWFKPVETKEGFDAYDEDGDKIAFMPKGGAFFDQTYFPYIDGYPKDYKDIRKAMEKVL
                     WQKLASSPWDNANKPDFFKTLREKAIYLRNTTDKAIMIGAGCNLFEWGTFLRRIDNFL
                     MDLILEPKEVENLLDKLMEIHLEGLAKICEAVGDVVDIVRFGDDLGTNEGPFMAPEIY
                     RKMFKPRHAVLCDYVHKHSKMKTYLHSCGSIRKLLPDLIEAGFEVINPVQTTARGMDP
                     AELKREYGKDITFWGGGCNTRWILNKATEQEVYDYTTHMLEILMPGGGYVFNTEHNIM
                     PEVPPQNILAMYKAVHDFKI"
     gene            complement(18746..19966)
                     /locus_tag="PLQ68_04400"
     CDS             complement(18746..19966)
                     /locus_tag="PLQ68_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013623338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXK71926.1"
                     /translation="MYIYRHIEEPVRKISKMFGAVLITGPRQVGKTTLLKRIAKNITY
                     VTLDDPIMISSAKEHAGTFFKDNPPPIFVDEIQYAPNLFPYIKMIIDSEHKKGQFYLS
                     GSQLFVMMKNVTESLAGRLGIIELLGLSLREIAGVKFNEPFIPTDEYFTARRKDIKEI
                     SYKDIWNIIYKGSMPQMYAESDVDWTFFYGSYVKTYVERDVRDLTQIGDESKFIKFMT
                     VIAASTGQLLNLASVARDVNISPPTAERWLSILQTSRIVYLLQPYHNNLIKRAVKTPK
                     VYFLDTGLAAYLTKWNTPEVLQNGAMAGAFFETFVIAEIIKSYLNKGKDISLYFYRDK
                     DMNEIDLLILENGTLYPIEIKKHADPSKKDITSFRVLDSIPNIKRGKGGVVCMYDNLI
                     TLSNEDMVIPVKYL"
     gene            20113..20763
                     /locus_tag="PLQ68_04405"
     CDS             20113..20763
                     /locus_tag="PLQ68_04405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK71927.1"
                     /translation="MNTLIVQANEILRNQQFEYAFCGGCAIDLFLGFESRIHGDIDIS
                     AYWKDRNNIIKHMWELDFLVYEMLGGGKAHHITDIENQKMIKRNIFCFQDTCDLIQLS
                     ASDEAGIYYINFRNTGQTKLDFIEFLFNSRNDTDFIYARNNEIKRALDKAILKNEGIP
                     YLAPEVCLLYKSTDITHEVNQKDFDKAITKMTDEQKDWLNDALIKTYPEGHKWINR"
     gene            20840..21088
                     /locus_tag="PLQ68_04410"
     CDS             20840..21088
                     /locus_tag="PLQ68_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008817563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04076 family protein"
                     /protein_id="HXK71928.1"
                     /translation="MAKVLLTITESNCRCKYFKKGDQFIVKDLCPPICHELWNTIYPS
                     VYALLNGAELDYGNKRLKCFDAKCPDEARVCIHGEVIE"
     gene            complement(21254..>21501)
                     /locus_tag="PLQ68_04415"
     CDS             complement(21254..>21501)
                     /locus_tag="PLQ68_04415"
                     /inference="COORDINATES: protein motif:HMM:NF014643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HXK71929.1"
                     /translation="YMTIDTKQIVTITEANQNFSRVTRIADKNGHVIILKNNKPKYIL
                     YDLDKNPIIDLTDDERIDIAAARILKKYLPALKKLAK"
     assembly_gap    21502..21601
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<21602..23535)
                     /locus_tag="PLQ68_04420"
     CDS             complement(<21602..23535)
                     /locus_tag="PLQ68_04420"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HXK71930.1"
                     /translation="MDTFSKLAPFIQDFIYQSKWEELRGIQVAACDIIFDTDDNLLLS
                     SGTASGKTEAAFLPVLTKLYNKPSKSVGVMYISPLKALINDQFKRLEQLLIDSHIPVT
                     KWHGDASVSQKNHLIKNPQGLLQITPESLESLITNKRGACLKMFSDLRFVIIDEVHYF
                     MRDVRGIQLLCVLERLKKLTGITPRRIGLSATLGDVSLAQNWLNTGTDRKCAAPIIDE
                     GKKRIGLHVERFVNYTEKRDLENSDSSDNTISSGGDIGTREHYEYLFKQTLDKKTIIF
                     TNSREESEFVIANLKEIALKNKAPDVYRVHHGNVSSLLRENTEDEMKSADEKIVTGAT
                     VTLELGIDIGSLDQAVQIGTPYSVSSFAQRLGRCGRRGQIPQLLFTFVESIRINTEDK
                     LGPINWDFIRTIAIIELYIKEHWLEPIPPQFHAYNLLYHQTISYLKSNGEMSAAALAQ
                     DILTLGCFKHISQDDYKYLLTHLLEIEQLQKTEQGGLIIGREGEKIVNSHKFLTVFIA
                     PEYLLVKDENRTIGTVDKVYPVGVRFALAGLTWETVDVNVKSKVIFVKRVPGISVVDW
                     DIDFKNDLHTVLVRKMRSVLLSDDSYPYLSERCKERLAEIRYITRNSGILDNLVTPLS
                     DKKYAVFPWVGTRQLITLNYA"
     gene            complement(23539..24855)
                     /locus_tag="PLQ68_04425"
     CDS             complement(23539..24855)
                     /locus_tag="PLQ68_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006293031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXK71931.1"
                     /translation="MTNLVPVRIANILINALKGGVVPRVGLEYITVGRSQEISTLLRD
                     IEMIEQGSASFRFIVGKYGSGKSFLLQTIRNYATAKGFVVVDADLSPERRFAGTKGQG
                     LATYKELIRNLSTKSKPDGGALPLILEKWISGIQSSIKAESDVTDSEFDKLVEKQIYA
                     VAGSLEGMVNGFEFAKAVVTYWKAYKEDDSAKKSNVLRWFRGEYPTRKEAKADLDINF
                     IVNDETWYDFLKIFATFLVGAGYKGMLVIVDELVNIFKIPNSITRANNYEKILTMYND
                     VLQGKAQNIGFLMAGTPQCIEDKYKGLFSYEALRSRLSEGHFASDKIKDLSAPIIRLQ
                     MLSQEEMYILVEKLRDIHSQLYNYKSSLTHDDLIYFLTVEYNRVGADTHITPREIIRD
                     FIEVINILHQNSELTVSGILGNNSFEMAKGGLSDEDIHSDFIEFEV"
     gene            complement(24852..>26258)
                     /locus_tag="PLQ68_04430"
     CDS             complement(24852..>26258)
                     /locus_tag="PLQ68_04430"
                     /inference="COORDINATES: protein motif:HMM:NF026952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB N-terminal domain-containing protein"
                     /protein_id="HXK71932.1"
                     /translation="LRFFDRRIQQDNSKVFYKQGMFMKDFEDDYEGNASFSSYFPYYQ
                     MLNYEQLRTYFTWRTNTRKGNISDTSLSYVFMYIYELLNNIGVENPKDGLSKILFFWN
                     KYKAINPAIDKYVIRWLKDYHIYYNMVQPFSDFAAEHNLTQYYPPMTDPDDNFILFCS
                     ISKYDIRKSVFFTDETESLISDCFSYIVKIIHKEFESAGIDFDNAFFRPTKKIVPWKP
                     FKDALFYNWCIKPDKRVVFSENEIYICSKNEWFFSSIITTDKGRSFVGYLMKKMEAEL
                     RKTVKYKYKLSANLDMVNPYTLDKLHKAGIFIDKIIESAVFDFYKEATKTVVRVDHEA
                     LDRIRLEALATQQSLIVEEQEKTYDFNTSIDCIDSVSSDQNIFSDPSDVEVSSNFDIW
                     DMLKDALNENELKALDVIIDNWDLKKFADESGIMLEVLVDGINEKAMDYIGDNILDED
                     MAIYEDYEEYIKGMVKTV"
     assembly_gap    26259..26358
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<26359..26689)
                     /locus_tag="PLQ68_04435"
     CDS             complement(<26359..26689)
                     /locus_tag="PLQ68_04435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK71933.1"
                     /translation="MGDNSNKKYGSYKIPDRQEYQKPAVKTAEKKPDTLPNGLFYEIE
                     YGNHPVEGVGRFVLKSQKIEPKKPDKIREIFEQMREISRHNASYSSDYLRFFDRRIQQ
                     DNSKVFYK"
     gene            complement(26702..27262)
                     /locus_tag="PLQ68_04440"
     CDS             complement(26702..27262)
                     /locus_tag="PLQ68_04440"
                     /inference="COORDINATES: protein motif:HMM:NF012920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HXK71934.1"
                     /translation="MKLGNRLLEVRENAGISRSQLSDFLLEKGFDVKPYTIGKWETGV
                     SNPSVEAFLAICDICKVSDIQQTFGDKRSLRLYDISVSAGCGNYLNSSDYDVIDVDNT
                     VPATADYAVRVIGDSMMPRFVDKQIIFVHEQPSLEDGEIGIFCLNNEAYLKKLDKGHL
                     VSLNPKYPPIPVKDFDDFKIFGKVVG"
     gene            complement(27705..28076)
                     /locus_tag="PLQ68_04445"
     CDS             complement(27705..28076)
                     /locus_tag="PLQ68_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014904155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK71935.1"
                     /translation="MCKMLLSINPEHVENIISGKKKFEFRKTRCRSSIDKIIIYATSP
                     VMQIIGEVDVTNTIIDLPEKVWSLTAEHSGISKSFFDKYFLNKEYAVAYELSNVQLYK
                     KPLKLEDLGLSYAPQSFVYIA"
     gene            complement(28051..29118)
                     /locus_tag="PLQ68_04450"
     CDS             complement(28051..29118)
                     /locus_tag="PLQ68_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013149550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK71936.1"
                     /translation="MAGKYKKIKFSEINLDDPFFDSLKDDYIEFPEWFRKKCSQEEYA
                     FVFTDEAGIGAFVYLKIEKEEIQLENNSLEPKNRLKIGTLCLAERCRGKRLGEGSLGL
                     ALWKWRDENLDEIYVTVFEKHILLIDLLKKFGFILAGTNNRGEHVYIRRRSEIYYSDS
                     YKSFPFINPNFTEAGIIPIYDTFHDKLFPYSELKNGNLDIEEETAGNGISKIYIATPY
                     KKMHYTVGEPVFIYRIYQGETGKTYKSAITSYCTISNVEIIKENNSIHISFDNFVKKA
                     GNKVAFSFDELEKIYSKQNVIMLELVYNGYFGKGHNVNHKTLKESGLFEDYPYNITLS
                     KNNFIKILRMGDVNVQNVIID"
BASE COUNT         9716 a         4698 c         5514 g         9134 t
ORIGIN      
        1 tctgcttatg ttgacataga tgtattcaga atgataaaat attcttttga agagctcgta
       61 tatgttgcag gaaaacggtc tgtgacgttt ctaccggggc gccttagacc cgactatgag
      121 tggcttattc cattgctttg attttttatg gtgcagtcac tacgaggctg cattttttaa
      181 tgggagggga atatgaaagt attagttcta atgggaagcg acagtgatta caaaacagtt
      241 gaacccggtc tgaaattttt tgaagattta aatattgaat atcaagtcag agtgtgttct
      301 gcgcatcgta caccgcatat agcagcagag ttggcttcaa atgcaaagag taacggtttt
      361 aaggttatta tcgcagctgc aggaaaatct gctcatctag caggagtttt agctgctcat
      421 acaatactgc cggttatagg tattccggta aagacaagtg atttaggagg aatggattca
      481 cttttttcaa cagttcagat gccatcagga gttcctgttg cgacagttgc tattgacggc
      541 ggtaagaatg cagcaatttt ggcagtacag atactggcat tgagtaacga agaattaagt
      601 ctgaaactgg aacagttcaa aaaagatatg gaacagcagg taatccaaaa ggatatggag
      661 ataaacaaga aataatatga gtggagtttt cggagtatat tcaaaagaca agtcggtaaa
      721 catagcagat acgatatatt acgggcttta tgccctacag cacagaggac agcaaagtgc
      781 aggtatcgca gtaagggata atgaattaat agagtggtat aaggctaaag gacttgttcc
      841 tgaagttttt aaaccaacag atttagaaaa gcttccaggc gatgcaggaa taggacatgt
      901 gagatattcc acatctggtg atgtctcaaa aggaaccata gagcctttag tgttgaagta
      961 cataagcgga tatatggcta tagcgaaaaa tggaaatatc attaattcca atgaattgag
     1021 agaggaactt gaagctaatg gatgcatgtt ccatactact aatgataccg aagttattgc
     1081 tgctttactg accaaattca ggataggaag cgagcacata gaagactctg tggagcagat
     1141 ggtgaagtat ataaaagggt catatgcaat tactatgatt acaccaagat acttaatagg
     1201 tttaagagat ccgttaggaa taatgccctt atgtatagga aaaataaacg attcaacata
     1261 catcatatcc agcgagtctg caggtattga tgctgtaggt ggacatttca taagagatgt
     1321 tgaaccagga gaacttgtat taattgattc aaatggaata cagtcaagaa aattgtttga
     1381 atgcaagaaa agcagtttat gtatatttga gcatgtatat tttgcgagac ctgacagtat
     1441 aattgacgga gcatcaacac atgatgcaag agtaaatgca ggaagaattt tagcaaaaga
     1501 gcaacctgtt gaagctgatg tagtaattgg agttccagat ggcggtacag atatggcttt
     1561 tggtttttca caaggaagcg gtataccggt tgcagcaggt cttttaagaa ataaatatgt
     1621 aggaagaact tttatcgcac caaatcagaa tgacagggaa attgcagtaa aaataaaatt
     1681 taaccctgta aaaactgaag tcaaaggaaa aagggtagtt ttggttgacg attcaattgt
     1741 aagaggaact acaaccaaga tacttgtcag aatgctaagg gaagccggag caaaagaggt
     1801 tcatcttaga atatgctcac ctccatataa attcccatgc tacttcggag ttgatacatc
     1861 aagcagaggg cagttgttag caagcaataa tgacaaagaa gctatatgta aatggatagg
     1921 agcagacagc cttgagtata taagttatga agggatacta aaagccccta taaattcaag
     1981 ggttggattc tgtacttcct gttttacagg aatatatcct atagatgtgc caaaagaagt
     2041 ttagaataat accttcagga ggataattaa tatgaaagat gcatataaag aagcgggagt
     2101 tgacgtaaga gctggttacg aatcagtagc tttgattaaa aaacatatac aaagaacagt
     2161 tataccagga gttcttggag gtataggaag tttttccggt ttgtttgaga tggatttgaa
     2221 gaagtatcct aatccagttc ttgtttcggg aactgacgga gttggtacga aattaaaact
     2281 tgcacattca ttaaaggttc atgacactat aggcatagat gcagtagcaa tgtgtgtaaa
     2341 cgatgtatta tgccagggag cagatccgtt gttctttctt gattacatag cttgtggaaa
     2401 aaattttcca aatataatcg agcagatagt gaaaggtgtt gcagagggtt gtatacaatc
     2461 tgagtgcgcg cttgttggcg gagaaacagc tgagatgcct ggtttctata aagaagatga
     2521 gtatgatata gcgggattct gtgtaggagt ggtcaacaaa ccagatatca ttgacgggtc
     2581 agcaatagaa gagggtgatg tgcttttagg tttagcttcc acaggtgttc acagtaatgg
     2641 gttttcactg gtcagaaaga ttttcggtga tgatgctcaa agcctaaata tgacatatcc
     2701 cggattttat aaatcaatcg gagacatact tttaaaacca acaaaaatat atgttaaaga
     2761 tattaaaaga attagaaata ttgcaaaaat aaaaggaatg agccatatta ccggaggcgg
     2821 ttttgtggag aatattccaa gaatgttttc tcaaagagaa gatcttacac cggttatcag
     2881 taaaggaaca tgggatatac cttccatatt tgaccttttg gctgaaaaag gcggaataac
     2941 agaggataat atgtatcgca cattcaatat gggtatcggt atggttattg ccctaagtaa
     3001 ggaagatgct agaaaagtaa aaaatgagct taaagatgat atacaagaga taggctatgt
     3061 agcagcagct aaagaagtca aagacaaaaa aaacattgaa gagaatatat atataagagg
     3121 ataggaaaaa tgattaaaag agcattaata agtgtaagcg acaaaagtgg tattgtggaa
     3181 tttgcaaaga aattaagtga catgaaagtt gagataattt caactggagg cacttcaaag
     3241 atcttaaatg aaaacggcat taaagtaata cctattgaaa acataacagg atttaaagaa
     3301 tgtttagatg gaagagttaa aactcttcat cctaatattc atgcaggact gcttgcaaga
     3361 agagatctta aagaccatat ggatacattg gatagtctgg gtataaatcc tatcgatatg
     3421 ctggttataa acctgtatcc tttcaaaaaa acaataatga aaagcgacgt aactaaagaa
     3481 gaagctatag agaatataga tataggcgga ccnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3601 nnnnnnnnnn nnatacagga aatgagatat ggcgagaatc ctcatcagaa agcttccttc
     3661 tataaagaac ccagaagaac aaaaggaaca atctgtgatg cagttcagct gcatggtaag
     3721 gaactttcat ataataatat aaatgatgct gatggagcgc tttccatgct taaggaattt
     3781 cctaatgaag tagcgtgtgt ggcagtcaag catgcaaacc catgtggagt aggaataggt
     3841 aagaatgtat ttgaagcatt taaaaaagca tatgactcag accctgtatc aatattcggg
     3901 gggatagttg ctttcaacag gactttagat gtagtaactg ctgaaaaact tcatgaaatg
     3961 tttcttgaaa tagttatagc tcctgattac aaggatgaag ctctttcatt attgaaaaag
     4021 aagaagaatt taagaatatt aagacttgat gacataacat atagtgacga atttgagtat
     4081 gttatgaaaa aagtaagtgg cggaatgctt gttcaaagca aggataatac tgttgttaat
     4141 atggatgatt taaaggtagt tacaaaaaaa cagcctacag aacaacagat aaaagatgcc
     4201 atatttggaa tggcagtatg caagcatgtt aaatcaaacg caatagttct tgtaaaagat
     4261 atggcttcaa taggaatcgg gcctggacag atgagccgca taggtgcagc aaatattgct
     4321 atatctctag ctggagaaag agcaaaaggc agtgttatgg catctgatgc atattttcca
     4381 tttgatgatt gtgttattga agctaaaaaa gcgggagttg aatgtattat tcatccaggc
     4441 ggttcaattt ctgacaaaga tgtaatagct aaagcggatg aattagggat ttccattata
     4501 tttacaggaa tgagacactt tttacattaa gaaaggcgga ttaatgaagg tactggttat
     4561 cgggggaggc ggcagagaac atgccgtagt taaaaaatta agtgaaagcc caaagattaa
     4621 taaaatatac tgcgcaccag gcaatgcggg aattagccag attgcccagt gtgtcaacat
     4681 aaaagctact gatatagatg gtatattgaa gtttgtagat gaaaacccca tggatatggt
     4741 tattgttact cctgatgacc cgttagcgct tggacttgtt gataagctta atgagaaaaa
     4801 aatacgtgct ttcggaccgg ttgcaaaagc tgccgagctt gaaagcagca aggctttttc
     4861 taaaaacctg atgaagaaat ataaaattcc tacaggggat ttcgaaattt ttgataatgc
     4921 tgaaaatgct ttaaaatatc ttaaagacaa aaaatacccg ttagtaataa aagcatcagg
     4981 actggcttta ggaaaaggtg taataattgt atcagaccag gaacaggctg agaagacaat
     5041 agaccagatg atgcgaaacg atatgttcgg tacttcagga catacagtca taatagaaga
     5101 attcttacaa ggaccggaag tcacagttct agcatttact gatggcaata caataaaaac
     5161 gatgccttca agtcaggatc acaaaagagc tttagatgac gacaagggat taaacactgg
     5221 tggaatgggt gcttttagcc caagccgtca ctatactaaa gatatcgaag attactgtat
     5281 aaacaatata ttctatccga caattaaagc tatgaaaaaa gaagaccgta attttaaagg
     5341 cgtaatatat tttcaaatga tgcttacaaa agatggacct aaggttattg aatataatgc
     5401 gagatttgga gatcctgaaa cacaggctct tctaccctta cttaaaactg atttatatga
     5461 tattttcaat gcaataatag atgaaactct tgataagatt gatattattt gggaaaacaa
     5521 agcaagtgct actatagtgt tagcaagcgg tggatatcca aaagaatata ttaaaggatt
     5581 taaaataacc ggtattgatg aagttcaaaa taacggtata actgtatatc atatgggaac
     5641 agctataaaa gatggaaata ttgttactaa cgggggaaga gtactttgtc ttactgcaac
     5701 agctgataat gttgaagatg cagttgcaaa agtatacaat aatattgaaa agataaactt
     5761 taaagatatg cactttagga gagatatagg taaaaaatga aactaggtat attaggagca
     5821 acattcgacc ctgtacataa agcacatctc cagatggcaa aatatgctct ggattactgc
     5881 gaatatgtac ttttaactcc ttgcactaaa cctcctcata aagatcatga cagtataact
     5941 gaagacagta taagatacat gttgctttgt ctggctttgg acgggtatga aaggcttata
     6001 gcttctgata ttgaacttaa aagaaaaaca accacataca ctatagatac actaagacag
     6061 gttaaagaag agtatcctgc ttttgacgaa attgtatata ttatcggagc agatacgcta
     6121 tttcagttaa caacatggaa agaacatgaa gaagttttca aattgtgctc ttttttagtt
     6181 tttgaaaggg aaggctttac tagaaagcag attcatgaga gaattgatat cctaaagaaa
     6241 gattacaatg ccaacattat atttgacata agaagcgtgt ctgaaatatc atctagcgta
     6301 atacggaaag ctatagtaga aggtgatgac tcaatagagt ggatgcttga caagagagtg
     6361 tataggtata taaaggaaaa tgggatatat aagtgagaaa gcaaaaaagg atataataag
     6421 ccgttacagc gaagaaagat acgaacatat tcaaaatgtt atcgaacttg cagttaagct
     6481 tgctgagaaa tataatgctg atgttgaaaa agcatatact gcggcccttt tacatgatat
     6541 agctaaagat atggaagtag atgagatggt tgcgcttcta gaatcggcag acatctttat
     6601 agacgaggaa taccataata aaaatatact tcacggacca tgtgcagcag tgatagcata
     6661 tgaaaattat gacatagatg atgaggaaat cctttatgcg atttattatc atactttcgg
     6721 cagagctggt atgggattaa tggaaaagat aatatttatc tctgatgctg ctgaaaaagg
     6781 aaggacatat aaaaatgttg gaaagataag aaaggccatg aacaccgacc ttaacatggc
     6841 aatagaaatg agtcttagcg ggactatttc tcatgtttta tctaaaggag aagtgctatt
     6901 tcctgcaact gctgcagctt tagagtattt taaagaacaa agaattaaga gataataatg
     6961 tataaatgta tatttaaaaa cgcatataag ataattaaga atgatgaaag ttaccttgcc
     7021 attagattta ctgaaaagca gttagaatat tataagaaca aatcagcagc tgcagaagac
     7081 agatcgcgtg atacggcagg tatttgcatg gatttctaca ctgatgcaga agaaataagt
     7141 tttgcatata aagcatgttg tttttccaga agatatgtag ggtttgattt ttacgaagac
     7201 ggcatattca gaaagcatat agaagaagct ttagacacca aacaatctgt aataactcat
     7261 cctttggaga aaaaagggta taaaagagta actgtatata ttcctaatct gagtgaaatt
     7321 accatgtacg acttaaatat agggaatttt aaaccgattg aagaaaattc agaaaaaaaa
     7381 cttcttttct taggtgattc aataacacag ggaatggtgg ctcaaagtcc ctctttagca
     7441 tttactacac agttagcaag agcatggaaa gcagaatctt tgaacagggg agtcggtgga
     7501 gcaaggtttg aagcagattc acttgatgat gaagatgaat ataatccaac ccatatattt
     7561 actgcttacg gtataaatga tatatatcac ttagaatgtg tagatgagct gccagctgtt
     7621 cttaatcagg ctgatatata tttgcaaaaa ctgaaaaaga tatataacga tgctttggta
     7681 tatgtaataa cacctatatg gtgtttaaga tatgatgaag ataagtattt tgcgaaaata
     7741 tttgatgaat acacaggcaa acttatgaaa atagtagaaa agtatggatt tacagttgtt
     7801 aatggtatga atcttgttcc acatgatact aaatattttc aggaccattc agttcatcct
     7861 gctgattcag gatttaatca ttatgcagag aatctgataa aagcaattga gtgacaaaat
     7921 aaatatttac tgataatata tgttataggt aagaagattc aaatatagtc aatggtggtt
     7981 tctaaggggg tagaagcatg ggtttacaga ataacgctaa aaaatgggtt cgtacatcaa
     8041 taatcataat gcttatagga attgcaatga taattgcagg tataactata gatgattaca
     8101 atttttactg gatgatttat gtaggtgcga tattatttct aacttttcta attgcatttt
     8161 ttatgtttgt aaaccaggca aacaggttag acaggttatt cagaggcgag gaacttctgg
     8221 ctaactggaa gttcgaggag aatgaaagac ttgctaaaac agaagaggaa ctgattcaga
     8281 gaaaagctca aaacaaagta atgctcctta taatagcggg attctttgtt gttattggtg
     8341 ctctgtttgt aatattcggg tttgatgatt ttgaagatgc tcttttgttt atacttatta
     8401 tggttggtgt tttagcattt atatctgtag tagctttaat tactccaggt tcacgttacc
     8461 gtaagatgaa gaatgctgct gctgaggtat ttgttggacc atatagtgta tgggtaatgg
     8521 gtgaatatgt tcaatggaaa gctccactga ctaagattac atctattatt tactcaaaag
     8581 acagagaaga taattacaca attacaatta actatttcat atggcaaaga tacgggcctc
     8641 agccgcacac atgcagagtt cctgttccat taggacatga aacagaagct gaatctgttg
     8701 ctagtgattt agccagtata aacaatgtaa aatatataaa taaactcata gatgctgaat
     8761 agtgctattt aacgctagta cttgatacat ctttgtagtt tactacggtt gatccagttt
     8821 tattaaccaa agctataatt ttttccttat tttcaggctt tgttattcca ccaccagaaa
     8881 tgataataat ctctacttct ttacctgaag gtagtatatt aactatgtta ttaaaagtcg
     8941 gagctggaaa acctgcccac attggaaatg caagaataac tttttcatag gaagaccaat
     9001 ccagattgat atccttaatg gttgtcggtt tctgagtaag agcttttgga cattccaaaa
     9061 atagatttcc aataaatgat ctttttttca atgtatctac ttcatataca tcagcttgtg
     9121 tctcttttgc cagggcatta gaatattttt tggttttacc accataggaa taatatacta
     9181 ttacagtctt cataaacttc acctcgtaac tttattctat taccaaaacg ttgcaatatc
     9241 aataaaaatt gatttgcaag aaaactagtc tgtttagttg taagtaaata gtcaggatat
     9301 attagatatt aagtgaaatt aaaaaaggaa agaaatgcat ctgctcctat agccatagcg
     9361 atataccagc aaattacaga taaaggcagt gcagttataa catctattaa gacatgttgt
     9421 tttacaagga ctgttgatag tattattaaa aatgataata caattaaaat gattttaaac
     9481 accaatgata atccgtcagc tgtcgcggct gctaatataa acagcattga taaagaacag
     9541 tgaagcgagg gcatacagct taaaatcttc ttatcgttag cgtaaataag ttttaccact
     9601 tttactgaaa atcctgctcc tttgggttca ggacggtaaa aagtagtggg atataccaaa
     9661 tatactatac cactgataac taaaaccatt aaaagggaca ttatgtacaa agcgtacgtt
     9721 tgtattgaat tatagaaaag aatgaaagga agtgaaatca ttacaaagta ccaggataca
     9781 tatgggaata taaaaactgg aataaaaggt atcttatcat caattttact tgaaagcatg
     9841 tgaggttttt ttacaagaaa ctgggtaccc cagtatgcta acatctgaat gataaatacc
     9901 cctatcaata tcaatattac tttaaataaa tcgttaatct tactatctcc ttactttttc
     9961 caaaagatta tatcacagac ttgatgtgct accaatattg tagtgcatta attgttaaga
    10021 aaaagtcaaa aaatatataa tatttcttaa tattacattt aattcaatat tttccattta
    10081 taacttctgc ttaatgttat ataatccaat tatataagga ggtaactttg gataatttcg
    10141 atacagagaa aatcaagaat gagatcgtaa aggtattaga ggacaaaaag gcaaaggatt
    10201 tgatatccat tgatgtgaag gatgtaacca cactaacaga ctatttcata atttgtactg
    10261 gaacaagcga caggcatcgc aaatcgttag ctgatgatgt tacagagaaa gcagaagaat
    10321 taggattaga agtatccgca ccgatagaag gatatgataa ggcaaagtgg atactgctgg
    10381 actgtaagga tatagtggtg aatatatttg acagggaaac cagagagaag tataaccttg
    10441 agaaactttg gagtgacgga gtacttgagg aaataagagc aaatagcaat aaagaggaaa
    10501 agaaatgatt ataaccggaa taagcgatga agcgggtaaa gatattgcta cgcagataaa
    10561 agcacataat aaacttggat ggactgcact ggagttacga aatcataatg gtgtaaatcc
    10621 atctacaacc ttgtttactg atgaaaattt tgattacata agaaaagtaa ttgaagagaa
    10681 ccagattact gttacaggat tcagttcagc tataggcaac tggagcagaa gtataagagg
    10741 cgatttcaat atagataaag aagacttaaa agtcgctgca aaaagaatga agatgctcaa
    10801 tgttaaatat atgaggatta tgtcatggat accggatgaa gatgatcctt catatacaaa
    10861 aaaggaagtt atacgaagaa gcagagagct tgcaaagatg gcagaggacc ttgatgttat
    10921 cctggcacat gaaaattgta caggctgggg cggagaaagc ccggaaaata tgctggaact
    10981 gaaatctgct gttgatagtc ctaattatgt tcttttatat gatacaggta atgtaactgc
    11041 tcatggnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnccaa ttccaggtca
    11161 ggttacatac ttcgtaggag gcatgctttt tggtacatgg ggagggtttt taataaatca
    11221 tactgcaacc atactagcct ctgtaacagt atactggctt ggaagagcgt ttggagaacc
    11281 attggtcgat aaactggctg gtaaaggtaa gactaaaaaa gcagtagatt ttgtcaagag
    11341 aaacggacca atattcttcc ttttagcttt tgtaattcct gggttgcctg atgatctttt
    11401 atgttatgtt gcaggtatcg tgaccctgcc ttttggaata ttcttatttc ttgtagcaat
    11461 agcaagaata ccttcaactt tgtcttcatc attgttagga aatggtgttc ttacatggac
    11521 aaccactcag actatactgg ttgtagcaat tcttgctgta tttatagcac ttgttgtggt
    11581 attcagaaag cctatcacaa atcttttaaa taagcttgtc aggaccaaaa gtaaagatga
    11641 gaatgtagat ataactgata tgatggaata gcaaaaatac tgatgacata atataaaaga
    11701 agggttataa tatctaaaca cggggtgtag cgcagttcgg tagcgcgctt ggttcgggac
    11761 caagaagtcg caagttcaaa tcttgtcact ccgaccacga agacatgctt tttcgctaag
    11821 gaaagggatg tcttctttta taaagaaaaa gtagaacatt gtgcaagtac ttttccattt
    11881 tgaaaattac ttgcaatatt tacaagtaat tgatataatg gttgcaaata gaggtgttaa
    11941 atgaataaac tcattgaacg taaagcatat ttagagcaac tctccatgtg gcgtgaagag
    12001 gaaatgataa aagtcgtcac aggtgttcgt cgttgcggaa aatctacatt gtttgaccta
    12061 tatatcgaaa aacttaaagc cgaaggcatt aaggaagagc agataatttt cataaatctg
    12121 gaggatcagg atttttctga actactagac tataaaaagt tgcatgatta tgtaataact
    12181 catatgcaga aagataaatg gacatatgta ttcattgatg agattcaaaa ctgtatagat
    12241 tatgaaagag ctatatctag tctatttcta agaaaaagtc ttgatattta cattacaggt
    12301 tctaatgcct acatgctgtc aggcgaactt gcgactaaat tgactgctcg ctatatagag
    12361 atagatatgc ttccgctttc cttttcggaa tatagtgaga tggtggatat ttacgacaag
    12421 cgcaagagat tcaatcttta catgaactta ggagcatttc cataccctgc tcgatttacg
    12481 gataataccc ttgcgcataa ccagtattta gagggtatct acaataccgt attggtcaaa
    12541 gatgtcctga cacgaaaaaa gttaaacgat gttactcttg taaaatctat agcaaccttt
    12601 ttatgtgata gtattggcag tcctgtttct gccaaaaaaa ttgcagacac actaacttca
    12661 agtggccgtc cgacaggtgt tgctactgtt gaattatatt tagaagcgct aactgatagc
    12721 tatttgtttt acaaggtgca gcgttatgac attaaaggga aaatgcatct caaatctgag
    12781 agtaaatatt atatctgcga tacaggtctt cgtaatatga tacttggggt gactaataga
    12841 ggtatcggtc atcagattga gaacattgtc tttttggaat tattaagacg tggttactct
    12901 atcaatatcg gtaaagcggg gcgtggtaca gaggttgatt ttgttgctgt tagagaaaac
    12961 aaaacagagt attatcaggt cgcagcatca gtattggatg aaaatacgct tgcacgagaa
    13021 cttcattcat taaaacaagt taaagataat tacccgaaat ttataataac gctggatgat
    13081 tatacaggag actatgatgg tataaggcaa atcaacctga tagactggct tgatacacag
    13141 tgatattact aattcacttc atagcaaaat tattcattcc aagtcggaaa gcattgaact
    13201 taaagcttgt ataatatttt acactacatc aagccttgtt tataattcgg gaccaagaag
    13261 tcgcaagttc aaatcttgtc acttcgacca aatgcacata atctgaacca ctcattaatc
    13321 tttacgattg gcgaagggtt cggatttgtg ctatatctgg attcgcaata agcagttttt
    13381 tggattcaga ttataatgaa ctttaagggt ctgattccga tgtgaatctt gattgaaaaa
    13441 gtatatggca gtagaaaaac acagtatttt tttaactgaa gttattgtaa tatattatta
    13501 tccatttgaa ggcggtaata ttatgaagaa acaagataga acggaacagc tctttactga
    13561 catgaagagt gtatttatgc ctagaaaaaa tctttctgct ttaggcgaaa agggtaaatg
    13621 gcgtattgtt gaatataaag caatgaaata gaattacccc agactattgt atatgcaatc
    13681 agtctatcat tgtcatattc cgtaaatatt acagcctgtt gcgggtttaa aaacgcttca
    13741 aatgtgtctt tactgcataa gtaatctgga aaacacttca catacaaacc atataaatca
    13801 tcaatcttca ttatttcctc ataatttcct atttcacagt attatcagct aagttaagta
    13861 tcagccatga gaagttagtg tagtcattca tgaacagaat tttgttctga tcttttaagt
    13921 gtattacatt atttgacaca tagtctttaa gctgtgttac attggctgtg tcatttaaat
    13981 ctacaagtac aagacactcc attctgtcag ggtatcttac agacataagt atatgggtat
    14041 ccattacata atagaagttt atcttatagt ccacatgata tagctgttgt ttgttctggc
    14101 ccagtttatc gcatttatat attgtaagac catttccttc tattgtcatg taatgaatgc
    14161 tatcttcatt tatataaaaa ttaccgtatg actcatcttc tactaccttc tttatatcag
    14221 ttccctcaac agtcatgcat aaaagctcat gctgttctgt gcggaaatat acaagattat
    14281 cggaaatact tattacttca acattatgtc cgagattttt aatacctgta ccatctgctt
    14341 tcatacggca cagattgtta taactgtctg catctgcata atatatatag tcgttataaa
    14401 ctaccataaa agagcatttt gcatcagaga taaatgtctg catacttcca tccgtcctca
    14461 tcttgtatat atgttcatct tttgataaat cagtaaagta gatccagtct ccaataacat
    14521 tcagattact tacttcattt tctgttaaaa gtgtccgtga agtaaagtct gtcttcattt
    14581 tgtataaaga atacccttcc tcagcattta tataataaac ccagtctccc tgaacacaga
    14641 aaacttctct tctgaactcc ctatagtcat ttatacccat tccacttgat aattcagtac
    14701 cagaactacc aacaatgttt atctgcccgt cagcataatc atggatgaaa ggccattcga
    14761 cagagagatc tgtctttgaa tattcatcct tgaatgtaat tatgaaatct ttatggtctg
    14821 agttgtccgt gctatatgac tcgactgttt ttcttaactc attattgaaa gctaccatgc
    14881 aagagatcag catctgcgtt tttaaccagt atatacgctg ttcttcggga tattcggaca
    14941 ttatctggtt cgcataacct aaagccagta tataatcttc gctagcttct gcttcatgga
    15001 atttggtata tagatcatca actgttaaaa ccggttcttc acttggaatt atttcagtaa
    15061 ctataggatc aagagaaggt tccgaagagg gactttcaat agttgcatct gatactgatg
    15121 gagactcgct tggagtccta ttaaaattgc atcctgacaa aagaataatt gctattaaca
    15181 gaatggataa gaataatctt cttttcatgt atttaccccg tttaaaattt caatgtattt
    15241 tagtaaatta tatcatcttt gcaattgtaa atatattatt ttatagtgat agaaattaac
    15301 atcaaatgaa cagaaatatg gacagaacta tacccaagtg atagaataag tatagttcat
    15361 ttattaagaa gacataaaac atcaaaaaaa catttcagtt acctaataag gggcatttaa
    15421 aatatatatt cagggaggga atttgtatga aaaagacaga tacgccgaat atccatcggt
    15481 ataatccgcc taaatggttt cgtgatgcag attttggtat ttttatccat tggggagcat
    15541 caagcatacc ggcatttgcg ccagttgatg ttgatgatta cgggactttg atgaaagaaa
    15601 agcctatgag ctatgttttt gcaaacatgc catatgctga gtggtatcca aacagcatga
    15661 aatttcctgg aagtcctgct cagctttatc atgaaaaaaa cttcaaggac aagacttatg
    15721 actattttgt cgaagaattt aaaaaacatg caaagaatgt ggatgtggaa aaatgggcag
    15781 aactgtttga agaagctggt gctaaatatg tggttgtggt aagtaagcat catgacggtt
    15841 ttgttatgtt tgataccaaa ataaagcatc ctaaaaagga aggttggcat ctggattttg
    15901 attttgtagg agatcttgct gcagctgtac gtaaaagagg gatgaaattc ggtgtatatt
    15961 actcttcatt aattgactgg tcattcaaag acagaaggat tgaaagtgca ggttctttgt
    16021 ttatggggaa tgataattca aagctttatt gtgattactg ttataaccac tggatggaga
    16081 taattgacag gtataaacct gaagttttat ggagcgatat tggttatccg acagacaaga
    16141 gattagagga tttgtttaaa tattactaca gcaaagttcc tgaaggtgtt gtaaatgaca
    16201 gatggggaca gtggccgaat tatcttagga ataagcttgg tgaagctttg ataaatagaa
    16261 tggcgcagag tgctctggaa aaaaatacag aagtagttct tgatacaaaa tattatgatt
    16321 ttaggacatt agaatacaat acaaaatggg aacagaaaga catatggttt gaagtatgcc
    16381 gaggaatgga taagtcattt ggatttaatc agttcagcag agagcaagac tatataactg
    16441 cagaagaggt gaaaaagata atttcagaag ttttcccgaa aaaggggcgt ttattactaa
    16501 atgctggtcc tgattcatac ggggtaattc cggaatatca ggctgagata ttaaaaaagg
    16561 ttggtaacag ataatgaatg acaagcataa gaaatatata gtcgaacaga tgatatggtc
    16621 attctttata aacggcatta taaacggggt tattgcatat tttcttaata agaacaagca
    16681 gtatgaaata cttacaatgc agcaaaacca tcttgacctc ataatagata tagcaataac
    16741 tgctatcatt cttgcctggc tcattgcatg gtctgttaat gcaggtgtaa aaaaagctgg
    16801 gctatatgca tcacttgaac caaggacaaa attccagtta tggatgggaa aatggtttaa
    16861 gatgccggca acatatggct ggatttggtg tattgccgtg ataccagtac tatatgggtt
    16921 atcagccctt ggaatattaa tattcagtat tacaaagttt actctttggg gctgggtaat
    16981 ttacaagacc gtatatactg catttttagg tgcaggtttt tcaagccttt ttataacaag
    17041 tgcatattat gcggatgctg ctaatcttga gcctaagaaa aagaaggggt gattctaatg
    17101 ctaaggatgg gttctgtcat acaagtaaag acggacaaac tggaagagta taagaaactg
    17161 catgcagatg catggccggg agttcttgat atgataagaa agtgcaatat ccgcaactac
    17221 tcgatatttt acagagacgg tctgcttttc agctattttg aatacatagg gaatgattac
    17281 gattccgata tggaaaaaat gggggctgat cctctgaccc aaaaatggtg ggcattgaca
    17341 gacccctgcc agatgccagt atcttctgca aaggaaggag aatggtgggc ttctatggag
    17401 gaagtgttcc atacggactg agtaattcaa tactagaggg aagataatat gaattcaaga
    17461 gagcgtatac ttacatctat taatcataaa gaaccagatc atgtaccatt ggatttaggg
    17521 tctactccca gttccaatat atctgcaatc gcatacaata atctgaaaaa acaccttgga
    17581 ataaatacag gacataccag gatatatgat gtcgtacagc agcttgccca gccagaggat
    17641 gatatattac aaaggttcgg agtcgatgtg ctggatatag gacgagcttt caatactctt
    17701 gatactgact ggtatgatat aaaacttctt aatggcagca ttgcccagta tccttcatgg
    17761 tttaagcctg tagaaaccaa agaaggcttt gatgcatatg atgaagatgg tgataaaatt
    17821 gcttttatgc caaaaggcgg agcatttttc gaccagacat attttccata tatagatgga
    17881 tatccaaaag attacaaaga tataagaaaa gcaatggaaa aagttctctg gcagaagctt
    17941 gcttcctctc catgggataa tgcaaacaaa ccggattttt ttaaaactct tcgtgaaaaa
    18001 gccatatatc ttcgtaatac gactgataag gctattatga ttggtgctgg ctgtaatctg
    18061 ttcgagtggg gtacattttt aagaagaatt gataactttc tgatggatct tatactggag
    18121 ccgaaagagg ttgaaaatct tcttgataag cttatggaaa tacatttgga gggtttggca
    18181 aaaatatgcg aagcggtagg tgatgtagtg gatatagtca ggttcggaga tgatcttggc
    18241 accaacgaag gaccattcat ggctcctgaa atttacagga agatgtttaa accacgccat
    18301 gcggtacttt gtgactatgt gcacaagcat tcaaagatga agacatatct tcactcctgc
    18361 ggttctataa ggaaactgct tcccgacctt attgaagcag ggtttgaagt aataaatcct
    18421 gtgcagacta cagcaagagg aatggatcct gcggaactta agagagaata cggaaaagat
    18481 ataaccttct ggggtggtgg atgcaatacg cgatggatac taaacaaagc aacagaacag
    18541 gaagtatatg attatactac ccatatgcta gaaatcttga tgcctggcgg gggatatgtg
    18601 tttaacaccg aacacaatat tatgccagag gttccaccac agaatatatt agcaatgtac
    18661 aaggcagtgc atgattttaa gatttaaggg ataactatat atactgttta tcttggattc
    18721 taaatagtag agaataagag tttacctata gatactttac tggaataacc atgtcttcgt
    18781 tacttaatgt tattagattg tcatacatgc aaacaacacc gccttttcct cttttgatat
    18841 ttggaatgct atcaagaacc cgaaaagatg taatatcttt tttacttgga tcggcatgtt
    18901 tcttgatttc aattggatat agcgttccat tttctaaaat caatagatca atctcattca
    18961 tatctttatc acgatagaag taaagagata tatctttacc tttatttaaa taactcttaa
    19021 taatttctgc aattacgaaa gtctcaaaaa aagctcctgc catagcacca ttttgtaata
    19081 cttctggagt attccatttt gtaagataag cagcaagccc tgtatcaagg aaatagacct
    19141 ttggtgtttt aacagcccgt tttataagat tgttatgata aggctggagt aaataaacaa
    19201 tccgggaagt ttgaagtatt gataaccagc gttctgctgt aggtggactg atatttacat
    19261 cacgagcaac tgatgcaaga tttaagagct ggcctgtact ggcagctatc acggtcataa
    19321 atttgataaa cttgctttcg tcgcctattt gagttaaatc ccgaacatcg cgttcaacat
    19381 atgtctttac ataggaacca taaaaaaaag tccaatcaac atcactttct gcatacatct
    19441 gtggcatact gcctttgtaa attatattcc aaatatcctt atatgagatt tcctttatgt
    19501 ctttgcgtcg agctgtgaag tattcatctg ttgggataaa tggctcatta aatttcacac
    19561 ctgctatttc acgtaatgat aaaccaagta attcaattat tccgagtctt cctgcaagtg
    19621 attctgtaac gttcttcatc attacaaata actgggaacc ggataaatag aactgtcctt
    19681 tcttatgttc agaatcaata atcattttta tatacggaaa aagattcgga gcatattgaa
    19741 tttcatctac aaatattggt ggaggattgt ccttgaagaa agttccagca tgttcttttg
    19801 cagatgaaat catgatagga tcatcaagtg ttacatatgt tatattcttc gcaattcttt
    19861 ttaaaagtgt tgttttacct acctgacgag gacctgtaat caaaactgca ccaaacattt
    19921 ttgatatctt tctaacaggt tcttctatgt gtctataaat atacataagc aactcctttc
    19981 ggctaatata acattcaata ttattttaga cgagttttct aaaaaaagca ataccgtaaa
    20041 atatcaataa taaactattg aaataggttg ctaattggtt tgtaacagta tcgtgatata
    20101 ataagaacct aggtgaatac attaatagta caagcaaatg aaatattgag aaatcagcaa
    20161 tttgaatatg cgttctgtgg gggatgcgct attgacttgt ttctaggatt tgagtcacga
    20221 atacatggag atattgatat ctcagcatat tggaaagacc gtaataatat aattaagcat
    20281 atgtgggaat tagatttttt ggtatatgaa atgcttggag ggggaaaagc acatcatata
    20341 actgatatag agaatcaaaa aatgataaag agaaacattt tctgttttca agatacctgt
    20401 gaccttattc agttgtcagc atctgatgaa gcaggtattt attatataaa tttcaggaat
    20461 acaggacaaa ccaagctgga ttttattgaa tttttgttta acagcagaaa tgatactgat
    20521 tttatatatg cacggaacaa tgaaatcaaa agagcactcg ataaagccat acttaaaaac
    20581 gaaggtatac catatcttgc acctgaagta tgtcttctat ataaatctac agatataact
    20641 catgaagtaa accaaaagga ttttgataag gcaataacca agatgacaga tgaacaaaaa
    20701 gattggctga atgatgcgct tataaaaaca tatccggaag gacataaatg gataaataga
    20761 tagataagta aaaaactgta aatgatataa tatgatttaa atatgtgata agaaactaat
    20821 attgttggga gaataaatga tggcaaaggt attgttgact ataacggaaa gcaactgcag
    20881 atgtaaatat ttcaaaaaag gagatcaatt catagttaaa gatttgtgtc ctccaatttg
    20941 tcatgagttg tggaacacta tatatccttc tgtgtatgca ttacttaatg gagctgaact
    21001 tgattatgga aataaaagat taaaatgctt tgatgctaaa tgccctgatg aagcaagagt
    21061 atgcatacat ggggaagtaa ttgagtgaag agaatgtaaa tttttactat attttcaaac
    21121 cttatacatg ctaaatagta cctcaaatcc cttcagtgaa atacctcaaa tcctttcacc
    21181 aaaatacctc aaatcctttc agttacatta ttcattacag attagagaag tgctttctcc
    21241 tttgagaatt taatcatttt gcaagtttct ttaaagctgg aaggtacttc ttaagaattc
    21301 tggctgcagc tatgtcaatt ctttcatcat ctgtaagatc gattattgga tttttatcta
    21361 aatcataaag tatatattta ggcttattgt tttttaatat aattacatga ccgtttttat
    21421 cagctatgcg tgtaactctc gaaaaattct gatttgcttc tgttatcgtt actatttgtt
    21481 ttgtgtctat agtcatatat tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    21541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    21601 ngcatagttt aaggtgataa gctgtcttgt cccgacccat gggaatactg catatttctt
    21661 gtctgataac ggggttacaa gattgtccag tataccgctg tttctagtta tataccgtat
    21721 ctcagcaagt ctttctttgc atctttcgct taggtaagga taagagtcgt ctgataacaa
    21781 cacacttctc attttcctta caagtactgt atggagatca ttcttaaaat caatatccca
    21841 gtccacaacg gatattccag gaactctttt aacgaatatt actttagatt ttacgtttac
    21901 atccacagtc tcccatgtca gtcctgcaag tgcgaaacgc acacctaccg gatatacctt
    21961 atcaactgtt cctatggttc ggttttcgtc ttttactaaa agatactcag gagctatgaa
    22021 tacagtcagg aatttatggc tgtttacaat cttttctcct tctctgccga ttattaaacc
    22081 gccttgttca gttttctgaa gctgctctat ttccaataaa tgagttagaa ggtatttata
    22141 gtcatcctgg gatatatgct taaagcagcc tagtgtaagt atgtcctgtg ctaatgcggc
    22201 agctgacatt tctccattac tctttagata acttattgtc tgatgataca aaaggttata
    22261 tgcatggaac tgaggaggga tcggttctaa ccagtgctct ttaatataca gctcaattat
    22321 tgcgattgta cgtataaaat cccagttaat gggtccgagc ttatcttcag tgtttatcct
    22381 tatactttca acaaatgtga ataaaagctg gggtatttgt cctcttcttc cgcaacggcc
    22441 taatctctga gcaaagcttg aaacactgta aggcgtccct atctgtacag cctggtccaa
    22501 tgaccctata tctattccaa gttctaatgt tactgtggcc cctgttacta tcttttcatc
    22561 tgcggatttc atttcatctt cagtgttttc acgaagaagc gatgacacat tcccatggtg
    22621 taccctgtac acatcagggg ctttgttctt taatgctatt tcctttagat ttgctataac
    22681 gaattcactt tcttcacggc tgtttgtgaa tattatggtt ttcttgtcaa gtgtttgttt
    22741 aaacagatat tcataatgct cacgcgttcc gatgtctcct cctgaactta tggtgttatc
    22801 actggagtct gaattttcaa ggtcacgctt ttctgtatag tttacgaatc tttcaacatg
    22861 cagccctata cgtttcttac cttcatcaat aatgggagca gcgcattttc ggtcagttcc
    22921 agtattaagc cagttctggg ctaatgacac atctcctaaa gtcgccgata atccgattct
    22981 tcttggggtt atcccagtta gtttttttaa cctctctaga acgcataaaa gctgaattcc
    23041 tcttacatcc ctcatgaaat agtgtacttc atcgattatt acaaacctta gatcggaaaa
    23101 catctttagg caggctccgc gtttattggt tattaagctt tccagcgatt ccggagtaat
    23161 ctgcaataac ccttgggggt ttttaataag gtggttcttc tgagacactg atgcatcccc
    23221 gtgccatttt gttactggta tatgggaatc tattaaaagc tgttccagtc ttttgaactg
    23281 atcgtttatg agggctttaa gaggtgatat atacattact cctacggatt ttgaaggttt
    23341 gttgtacagt tttgtaagca ctggcagaaa ggcagcttcg gtttttcctg aagcagttcc
    23401 tgaagagagc agaaggttgt catcagtatc gaaaatgatg tcacaagcgg ctacctgtat
    23461 gcctcttagt tcttcccatt tgctctggta tatgaaatcc tgtataaaag gtgcaagttt
    23521 tgagaatgtg tccatttgct atacctcgaa ttctataaaa tcactatgta tgtcttcatc
    23581 tgacagaccg ccttttgcca tctcaaaact gttgtttcct aatataccgc ttacagtcag
    23641 ctcagaattc tgatggagta tatttattac ttcaataaag tcacgtatta tctctcttgg
    23701 cgttatatga gtatctgctc ccacccggtt atactctacg gttaggaagt atataaggtc
    23761 atcatgtgtt aaagaggatt tatagttata cagctgcgag tgtatatctc taagcttttc
    23821 aaccagaata tacatttctt cctgagatag catctgcaaa cgtattatcg gagcagaaag
    23881 gtcctttatt ttgtctgatg cgaaatgtcc ttcagaaagg cgggaacgta aagcctcata
    23941 actgaataat cctttatact tatcttctat acactgagga gtgcctgcca tcaaaaatcc
    24001 aatattctgt gcttttccct gaagtacatc attgtacatt gtaagtatct tctcataatt
    24061 atttgctctt gttattgagt taggtatctt aaaaatatta accagctcat caactataac
    24121 cagcatacct ttgtatccgg cacctactaa aaatgttgca aatatcttta aaaagtcata
    24181 ccatgtctca tcatttacta tgaagttaat atccagatct gctttagctt cttttctggt
    24241 agggtattct cctcgaaacc acctgagtac attggacttt ttagcagaat catcttcttt
    24301 gtatgccttc cagtatgtta ctacagcttt tgcaaactca aaaccattaa ccataccctc
    24361 caaagaacct gcaactgcat atatctgttt ttcaacgagc ttatcaaatt ctgaatcagt
    24421 aacatcagat tctgctttaa tggatgactg tatgcccgat atccattttt caagtatcaa
    24481 aggcaatgct cctccgtcag gttttgactt agtggaaaga ttacgaataa gctctttgta
    24541 tgttgcaagc ccctggcctt tagtacctgc aaaacgccgt tcgggagaaa ggtcagcatc
    24601 tacaacaaca aatccctttg cagtggcata atttcgtatt gtctggagaa gaaagctttt
    24661 tccgcttccg tacttaccga ctataaatct aaaagatgcg cttccttgct caatcatctc
    24721 tatatcacgc aataatgttg atatttcctg agatcttcct acagtgatgt attccagtcc
    24781 tacacgcgga actactccgc ctttcagagc atttattaag atgttcgcta tccttaccgg
    24841 taccagattc gtcatacagt ttttaccatc ccttttatat attcttcata gtcttcatat
    24901 attgccatat cttcatcaag gatattgtcc cctatatagt ccatggcttt ttcatttatg
    24961 ccgtcaacaa gtacttccag cattatgcct gattcatctg caaatttctt tagatcccaa
    25021 ttatcaataa tgacatcaag tgctttaagc tcattttcat ttaatgcatc ttttaacata
    25081 tcccagatat cgaaattaga tgaaacttca acatcagaag ggtcagagaa tatgttctga
    25141 tcagaagaaa cagaatctat acaatcaata gaggtattga aatcataggt cttttcctgc
    25201 tcttcaacaa ttaaggattg ctgagtggct aaagcttcca gcctgattcg atctaaagct
    25261 tcatggtcaa ctcttaccac tgtttttgtt gcttctttgt aaaaatcgaa aactgcagat
    25321 tcaattatct tgtctataaa tataccggct ttatgtaatt tatctaatgt atatggattt
    25381 accatatcta aattcgcaga taatttgtat ttgtacttaa ctgtttttcg taactctgct
    25441 tccatttttt tcatcaaata accgacaaaa gatcttccct tatctgttgt tattattgag
    25501 ctgaaaaacc attcattttt actgcatatg tatatttcgt tctctgagaa tactactctt
    25561 ttatcaggct ttatgcacca gttatagaaa agtgcatctt taaatggttt ccacggtact
    25621 attttcttag tagggcggaa gaatgcatta tcaaaatcta ttcctgcgga ttcgaactct
    25681 ttatgtatta ttttaacgat atatgagaaa cagtcagata tgaggctttc tgtttcatct
    25741 gtaaaaaaca cagacttcct tatatcgtac ttggatatgg agcagaacaa aataaagttg
    25801 tcatccgggt cagtcattgg agggtaatat tgtgttagat tatgttctgc agcaaaatct
    25861 gaaaagggtt gtaccatgtt gtagtagata tggtaatctt tcaaccatct aatgacatat
    25921 ttatcaatag caggattaat ggccttatac ttattccaga aaaacaatat cttggataag
    25981 ccatcttttg gattctccac tccaatatta ttcaatagtt catatatata cataaagaca
    26041 taagacaagg atgtatcaga tatatttcct tttcttgtat tagttctcca tgtgaagtat
    26101 gtacgaagtt gctcataatt gagcatctgg taatatggaa aatatgagga gaaggaagcg
    26161 tttccttcat aatcatcttc aaagtctttc ataaacatgc cttgcttata gaagaccttg
    26221 gagttatcct gctgtattct tctgtcgaag aatctaagnn nnnnnnnnnn nnnnnnnnnn
    26281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    26341 nnnnnnnnnn nnnnnnnngc ttatagaaga ccttggagtt atcctgctgt attcttctgt
    26401 cgaagaatct aaggtaatca gatgagtaag aagcattatg ccgtgatatc tctctcatct
    26461 gttcaaatat ttcgcgtatc ttatccggtt tcttaggttc tattttctgg gatttaagaa
    26521 cgaatcttcc cactccttct acaggatgat taccatattc tatttcataa aaaagaccat
    26581 taggaagagt atcaggtttc ttttctgcag tttttactgc tggtttctgg tactcctgac
    26641 gatcaggtat tttgtatgat ccatatttct tattcgaatt atctcccaca atccccccag
    26701 attaacccac aactttaccg aatatcttaa aatcatcgaa atccttaacc ggtataggag
    26761 ggtatttagg attaagagat actagatgtc ctttatcaag tttctttaaa tatgcttcgt
    26821 tattaagaca gaatattccg atttcaccat cttccaaaga aggttgttca tgaacaaaga
    26881 ttatctgttt atccacgaaa cgcggcatca tgctgtcacc aataactctt actgcataat
    26941 cagctgttgc aggtacagta ttatccacat ctattacatc ataatcagag ctattaagat
    27001 aattgccgca gcctgctgat acagatatat catacagacg taaagatctt ttatctccga
    27061 atgtctgctg tatatcagaa accttacaga tatcacatat agcaagaaat gcttctacag
    27121 atgggttaga tacgccggtc tcccatttgc ctatagtata tggttttaca tcaaaacctt
    27181 tctctaaaag aaaatcactt aattgtgaac gtgaaatacc agcattctct cgaacttcca
    27241 gtaatctatt accaagtttc attttttcgc ctccacctct taattgtata tgctaagaaa
    27301 taaaaagcaa tataaaaatg cagaaactgc acgaaaaata caataagagt gcagaaaatg
    27361 cgaaaagatt agttgaatag cagtttttgt ataaagaagg gaatggtgat attaaagata
    27421 tttaattaaa taatatagaa aatagaagtt acacattatc tcattatctt agcgcattta
    27481 gctacaagca caaatggcca aaatagtatt aatagtatta actttgttat ttcaattgct
    27541 ctgatcattg atggttcccc tttaacatta ttctctctga catatcaata atagtatgag
    27601 gcatgtccaa taagtgggac taataatggt gtttccctca gatatgtaat gcaagagtat
    27661 aatataatga tgttatgaat taatatttta aataattaac tctcttaagc tatataaaca
    27721 aaagattgag gtgcataaga aagtcctaaa tcctctagct ttagcggctt tttatataat
    27781 tgcacattgc ttagttcata agcgactgca tattctttgt tcagaaaata tttatcgaaa
    27841 aatgattttg atattccaga gtgttcagct gttaagctcc ataccttttc gggtaaatct
    27901 atgattgtat ttgttacatc gacttctcca attatctgca tgactgggga tgttgcatat
    27961 attattattt tatcaatgct cgaacggcaa cgagtttttc ggaactcaaa cttcttttta
    28021 ccacttatta tattttctac atgctctgga ttaatcgata ataacatttt gcacattaac
    28081 atcacccatc cttagtattt ttatgaaatt attcttagaa agcgtaatat tatatggata
    28141 atcttcaaac aaaccacttt cttttaatgt tttatgatta acattatgtc ctttaccaaa
    28201 gtaaccatta tagactaatt ctagcatgat gacattttgt ttactatata ttttttccaa
    28261 ctcatcaaaa gagaaagcaa ctttattacc tgcttttttt acgaaattgt caaaagaaat
    28321 atggattgag ttattctcct ttattatctc aacattcgat atagtacagt atgatgtaat
    28381 agcggatttg tatgttttgc ctgtttctcc ttgatatatt cgataaatga atacaggttc
    28441 tcccacagta taatgcatct ttttatatgg agtagctatg tatatctttg atattccatt
    28501 acctgcagtt tcttcttcaa tatccaaatt cccattcttt aattccgaat atggaaatag
    28561 tttgtcatgg aaagtgtcat atattggtat tatgccagct tcggtaaagt taggatttat
    28621 aaaaggaaaa gatttataag aatctgaata ataaatttct gatctgcgtc ttatgtaaac
    28681 atgctctcct ctattatttg tgccagctaa gataaaaccg aattttttaa gtaaatcaat
    28741 taacaaaata tgtttttcaa aaacggttac ataaatttca tctagattct catctctcca
    28801 tttccacaat gccaaaccaa gagaaccctc acctaatctt ttacctctac acctttcagc
    28861 caaacaaaga gtacctattt ttagtcgatt ctttggttcc aaactattat tctcaagttg
    28921 tatttcttct ttctcaattt taagataaac aaatgcacca ataccagctt catctgtaaa
    28981 aacaaatgca tattcctctt gggaacactt ttttctaaac cattctggaa attctatata
    29041 atcatctttt aaagaatcaa aaaatggatc gtcaaggttt atttctgaaa actttatctt
    29101 tttatatttt cctgccataa tcacctcttt aaaatctctg atgtagcttt ggatttgcac
    29161 ttttatatgt gcaataaaaa tttacttata caatccatgt ctgctcttat tttatcagat
    29221 aattaacata aattactaat aacatatgtc aatataatta aatataatac aaatactaat
    29281 atctgttgat agataataac caaattaaat gatctgtaag agcattattg atttcttcct
    29341 ccgattatgc aatagttata attgataata tgttttcttt atataattct ggaggccatc
    29401 atgagattag tcataatcgg agcagtagca gcaggaacct ccgctgcagc aaaggcaaga
    29461 ag
//
LOCUS       DAOSXM010000020        27474 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2111
            NODE_1107_length_27474_cov_6.775922, whole genome shotgun sequence.
ACCESSION   DAOSXM010000020 DAOSXM010000000
VERSION     DAOSXM010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864178
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 27474)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27474)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:24:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,261
            CDSs (total)                      :: 2,229
            Genes (coding)                    :: 2,218
            CDSs (with protein)               :: 2,218
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 3 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27474
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1107_length_27474_cov_6.775922"
                     /isolate="AMR_MDS_2111"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            79..597
                     /locus_tag="PLQ68_04455"
     CDS             79..597
                     /locus_tag="PLQ68_04455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK71937.1"
                     /translation="MSTKIYNAYRVKGMSLEELFMFLVALRKQYHKECIKEIKACYGD
                     NLPKWFEVQKELERQLSSGTNDALNFQASAVIYPYKKRVYIQFFGLRERYLEKVLDGR
                     FEDYHYQNQADWPEEISEKQWNRRGLVWDKILSLGQDGIPSDNGFIFPIYEKQHCLYI
                     AYSIGQLERPEG"
     gene            727..906
                     /locus_tag="PLQ68_04460"
     CDS             727..906
                     /locus_tag="PLQ68_04460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK71938.1"
                     /translation="MEVTSDKRLTIFIELNENEADWLMTFIQNHPDLEHENQTARDIR
                     IKLFNELRMHLDKFH"
     gene            1022..1414
                     /locus_tag="PLQ68_04465"
     CDS             1022..1414
                     /locus_tag="PLQ68_04465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK71939.1"
                     /translation="MRETTEVVKSADGGQWEFKMTLPETIDEACEIFGKDGALDIFNS
                     GLKVKKQNIAREGFKNGKDIDEVESLCEAYRPGEKKKNQGEIFTELVMENAEQLRMDP
                     DLKKEILDLYKTGKVKDAIDRLRTLSED"
     gene            1485..1706
                     /locus_tag="PLQ68_04470"
     CDS             1485..1706
                     /locus_tag="PLQ68_04470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK71940.1"
                     /translation="MNAFEALLHGKDITTLTEEEINDLMAQMSSDELAKFEQVVKAKT
                     PRRVKESKTSKKAEDYLNSLITKGLSKKG"
     gene            1709..2488
                     /locus_tag="PLQ68_04475"
     CDS             1709..2488
                     /locus_tag="PLQ68_04475"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HXK71941.1"
                     /translation="MLLRSSSFSTFKVCPRKAYYAYILGLVKKNGFNIDLFFGSIIHD
                     AVDVFNKTGDIEDALNLLEQSDWFHYHSKKNKFVALTLLKLYARFISGHSIETSEEEF
                     TFDVGNHIWKGKFDSLYKPSNSTYVGEVKTTNRWFLHTDPNDQFISYYIGAKRKYPRL
                     QGVYIFNFDPEKVDVGGTIFVPSQFKIDEWLEETKVAADYFQLCIDKETFPKNPAGCF
                     IYGKQYPCDFLDLCTSNDYGTRQAIIKNNFVVNEEAVNLSW"
     gene            2515..2700
                     /locus_tag="PLQ68_04480"
     CDS             2515..2700
                     /locus_tag="PLQ68_04480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK71942.1"
                     /translation="MIQSKSKYNICVLDSNEKIYELKDVEMPVLTCSDCIETFITLKV
                     IAENDTITLKGEDYGKF"
     gene            2687..3544
                     /locus_tag="PLQ68_04485"
     CDS             2687..3544
                     /locus_tag="PLQ68_04485"
                     /inference="COORDINATES: protein motif:HMM:NF024870.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HXK71943.1"
                     /translation="MGSFKDEAVGKFMVLFWGPPKVGKSVLASQFPNLYYVNVDDGLG
                     SVKAMTTKQGIDIDFKHFNVGSGKTTDKDYIELCGEKMAMNSSAWTKNKKLAEMLAKT
                     CKEDDTIVIDHLARIGEFLQRHIERSTGNRPLQIQDWNTFTMELTDYFDYFKDEACKA
                     NVIILGHDNYYEEKATGELRKLLLLPSSMRQRIPGFVSEVLYIHTEVSGGKRNRQFTR
                     HLQSMPDRMTATGSRSLIPDMENPTYLKMKPYLEAYLGKKLPEPTWTPDEKLFGEGIV
                     EKEVKNITK"
     gene            3560..3940
                     /locus_tag="PLQ68_04490"
     CDS             3560..3940
                     /locus_tag="PLQ68_04490"
                     /inference="COORDINATES: protein motif:HMM:NF016896.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF669 domain-containing protein"
                     /protein_id="HXK71944.1"
                     /translation="MPQISLDYDLENVSSEFTPLEPGTYYGRIESVELTKSQKTNKPM
                     LKIVWIITEGEYEGRKLFDNVLLETEWKVKQYALLAGIPSGTELDTDDFKDCEAILHV
                     IQKPNPQGGEGEMTNNIKKIEAIS"
     gene            4007..5299
                     /locus_tag="PLQ68_04495"
     CDS             4007..5299
                     /locus_tag="PLQ68_04495"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HXK71945.1"
                     /translation="MKIPVSDIIIESDFREDRVDIGDLALSIMNNGLLSPIILTNAVD
                     GKYQVIAGRRRFIALRDYINLEELEENEHFVFRDKVDPLVVQFEENFRRLDFTPIETA
                     RLVSAIHKKYVDIHGEAVKGIKGKGWSMDDTGRLLSKDKSFVSRMLLFLRHEEDIKNC
                     TNIRDAITTIEKMRSKNITDIIQKTRVEKALEEKITGKIEDYISNLYLGDALDFLPTV
                     KKESVDFILTDPPFAINYDEIAGGESFTTYDDDPEEILKLIDSCMSHYYRILKKNRFI
                     VVWVAFEHMNTILDMMDTAGFYTSATPLVWVKLNASGRSSNPDKNLGSACEIAIYGWK
                     GTKAELVTKGRGNVFPYDTVRKNRIHIAQKPESLLCDILNIFSNPGDTVVDTFVGSGS
                     TLRSCFLADRFFRGCEKLEVNYNSTIVYTRDWAKTINK"
     gene            5464..7788
                     /locus_tag="PLQ68_04500"
     CDS             5464..7788
                     /locus_tag="PLQ68_04500"
                     /inference="COORDINATES: protein motif:HMM:NF012688.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HXK71946.1"
                     /translation="MTLIQYIPKESSKICFVGKAPGEVEMRTRVPFSGPEGFLFNKLL
                     RLAGIERTSVYITNTVHVRPTKNIYETLSKQTIEDGKDQLISDLSAWKEKGLTTVIAV
                     GGRALEMLTGASSVIKYRGTAFPCAIVEGLKVYGTLHPEDLIRGNGLYEPIVVADIKK
                     AVADSETSEVFYPERNIEILHFPEQAINKLESVTAIEKPVTVDIETSGPVMTSYGVGL
                     SANNAFVIPYDLLKLPALLKAIGKFAMSNTPKIFHNALFDVFHNAYYYKILNRNIYFD
                     TMLAQHAVYPTYPKSLAFCGSIYTKEPYWKDEGKSVFSQLSSKDALMNSSLWDTLYIY
                     NGKDCCLTYEVYEALLTELEDWKVRPTFELSMELLQPCLYAMLTGLRVDELAVQEFAD
                     KNEKAIEIMERIKEHAIGPINIKSHKQLKELIYDTWRMPVQKKQGKITTEDKKLARLA
                     SFPTPYKARIELIRKLKESYKKRDFYTLKLDTDGRIRYALNIGGTYTGRMSSSKSITG
                     SGMNFQNQPKEVRSFYIADPGEIFVQVDLSQAEARVVAALCKDIEWLEAFDKEDLHSK
                     TASFLYNIPIEKVEKKTHRQVAKRVAHGTHYGLGKMLLSEILECSMGEAERLKNKYHN
                     MHPKLHEWQERVHKTVKDKKVIVTPFGRVMQFFGPLNDSRLREAVAAEPQSISAEYLN
                     RGLIRCWKTIPNFKFKLQVHDSILVAVKDDINIINYTMLKMKRLIEQKIDVHGIEMII
                     PCDFEIGYSWGKMVEVKDLAKVKEIYETLQKKDM"
     gene            7788..8963
                     /locus_tag="PLQ68_04505"
     CDS             7788..8963
                     /locus_tag="PLQ68_04505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK71947.1"
                     /translation="MKYNRFLECYRFYTRGNETPELFHLWCGLSALAGACEKRLWLDR
                     GFFNVYFNLYVVLISPAGLCAKSSSLDLAGKLLKDAGYRLLEGSVLKEKIIIEMLESM
                     KTVQLDVESIFPHCSVTYLSDELNVLLSSGVDMIKFLVDMYSKDDLYAYKTKNSGTFE
                     LPYPYFNLLTAAVPQWFGPQLAGDMASTGFLARCILIYLDQKRGKYPQPIYTKEQVDA
                     RAECLEMLTSIADWYGPLELSESAKKFYDDWYMSQDISPSEDYRIAAYLERRNKVHIL
                     KVAGLMAVGDLRKIIQKIDFERAIDLFKMTEKRLRMSYLIAGGNKLAQYVYKIQGLLK
                     DNGGKLYTKDLMKMFYTDLNPADFKICLQTLVEMNEIELVQEKSTKLYYINKLKGEN"
     gene            8965..9420
                     /locus_tag="PLQ68_04510"
     CDS             8965..9420
                     /locus_tag="PLQ68_04510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK71948.1"
                     /translation="MGFIGFRTSDKLQQLIEKAAKTRGLSVSTLCRDIIRDNLDTTMG
                     YSNWKAIAWNLFNITADQLNYALNKATCPDDFFSIVSKWSKVTKIQIIPGKENVTLKF
                     EHQGEAYIIREPRNMPKLVTIANIIELLDTKIYETYMKGEADGNKKDNN"
     gene            9395..9772
                     /locus_tag="PLQ68_04515"
     CDS             9395..9772
                     /locus_tag="PLQ68_04515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK71949.1"
                     /translation="METRKITINISEEQAKHLDKLVELLNSDYSKIIRNMLDDYAVNW
                     NIDKKFAEDCQLASIYFLDFTIYLKCRMVTEIIKKNSNYKVDPYVERSLHEKLESLKT
                     VFSTNRDFLSFISWYRLLLKTAE"
     gene            9777..10160
                     /locus_tag="PLQ68_04520"
     CDS             9777..10160
                     /locus_tag="PLQ68_04520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK71950.1"
                     /translation="MNRKFIYVTGPFEGLNEYAKKANITQLDLVCRRVIVAKHIPLCP
                     ILFYGAWMKDPRFYSQSAWWVDNIFKPIMLQCNYFCYIPPYAGVRNERLEYEKDIWKT
                     LNRTRMIIADRLIKYFNEDVDNGHN"
     gene            10147..10566
                     /locus_tag="PLQ68_04525"
     CDS             10147..10566
                     /locus_tag="PLQ68_04525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK71951.1"
                     /translation="MDITKVNSFERLEEAFLDELFVRYIARRFLDLSSLRAVAVELDL
                     NVNSFDRLFTVDPKHENIFNKYLDEEIDKADILLSKASKIIARQRLFDILREHAGADN
                     KEVIQAAKAILTSVNVKDGGKSDGFDKILEDMITREE"
     gene            10563..12047
                     /locus_tag="PLQ68_04530"
     CDS             10563..12047
                     /locus_tag="PLQ68_04530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK71952.1"
                     /translation="MKEQRQRKIRQIVKILNAKYAEDFHGWVRDNIDFSGLDFDDLTW
                     QQIEIGNAIIHDKNVCVAGGGGIGKTAVAALLILWFLSSFLDAKIPTTAPSGKLLDDI
                     LWSEISTWLNRCKLKHLFELKSGKLQIKEFKEWYAVARTVPRDGKQLNDTLAGFHAPN
                     LLIMVDESSGVPNPVFTALDGAMTKENARILLISNPVSTGGYYYDTISDPKGKGKGFK
                     VLFFDARESPLVSKTYEERIIARYGQDSPMYKAKVLGLPIDLEDTLLISPRQFDDLIA
                     RNKDRAYGRVVLGVDVAGRGTDMSVICNRHGQSIVAWDEFNTNDTVLLKNSIYRIIES
                     RYKNYDVVVVVDGVGIGSGLCDLLESERRYKIIRFIGSEKAHDTNMYDRKRTEGYYTL
                     KKVIMDTHFPVKPPERLKKELVNIKFNYDKGPISMQAKEKLIDILGFSPDYADALMLS
                     SVVDNFSVGSVGLFMPQSINNRLSKILVTQKQANRFGKYSRFLQ"
     gene            12060..13346
                     /locus_tag="PLQ68_04535"
     CDS             12060..13346
                     /locus_tag="PLQ68_04535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK71953.1"
                     /translation="MFGNNKLQKEIDQIKHELFEVKKANDAFVSRAKMGSLGTEDFMK
                     GTSNLEFLTELEPPTGLQLYYKMYRNEPIIGGIMLLLYNILSGLSWELTGSNKAFIKD
                     MFDNFPVDMSSIFFDMASALCFGFYVGEMLITVDNGRIILVDIEPRFQTTIEKINNEE
                     GNVVQQCTEGVYNIPYTKCVHHTFLASNRNPFGDSIIRAAYKPYYYKLSVEASEATGL
                     ERDLTGLPILTAPENFDFTAALETSPTYDPNAAETLQWAISLVGAVRKDSQQGIVKPS
                     GWDFQIVRGENRTTTPTNEIIARYNTEMAISTLANFLALGAFASTNNANVGVHLNNFV
                     TCCESYARKMCTTLQKVINRLCKYNNMTAPTLSLFFPNKSALKEVGEFVDKLTKNQVI
                     RPTDSLEKSLLMLANLPYEKQKGDRNAKRDTENTDQ"
     gene            13312..13944
                     /locus_tag="PLQ68_04540"
     CDS             13312..13944
                     /locus_tag="PLQ68_04540"
                     /inference="COORDINATES: protein motif:HMM:NF025901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdF-like putative serine protease
                     domain-containing protein"
                     /protein_id="HXK71954.1"
                     /translation="MPNVTQKTQISKELYRAYSSQERRRKLHADNFLLITKDGNRKFP
                     VKNLDGKFNKELVVAAIASAERYNYTMVAKEAARIYDKYFKDSDPTFDFSIQEHDAEG
                     KEIFGIVLEPDYKDADGHVFDAITIQEACYEYNSNFSNTAYRHTVRLNKNEVTLVESY
                     TAPTDFKLNDKEVKKGTWLQRWLIHDENIRKDILTNKIVGFSIGGYLISE"
     gene            14079..14855
                     /locus_tag="PLQ68_04545"
     CDS             14079..14855
                     /locus_tag="PLQ68_04545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK71955.1"
                     /translation="MAKMKMKLRVHEVSLAANPKNRQRFILVKDKGGIMNKLANFLAL
                     IASKADVSEVIKEELKDTDTKLNKTDIIACLKDLGISFEAKDFMAEGESVVKTAEVEI
                     VKKDSDMKVVDTTKFDVLAKDSYTLKEEHKYANLDPEIRKDIEDSKKTILQLRKDRFV
                     NDVVQKIGKEHAEKFEVLFDSIESDALDSILNVVVSLQETIKDLGKKVGSVELDGLNE
                     NEATITREVEKLAKDQKISVSDAWVEYAKANPEKMKSLDH"
     gene            14876..15265
                     /locus_tag="PLQ68_04550"
     CDS             14876..15265
                     /locus_tag="PLQ68_04550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK71956.1"
                     /translation="MSKYEQYVEKRNLVSSTTLADQEGRVVTYAGALTTQKGDIAAGV
                     VETGNGASEASVIYSRGHCEAYCDGAVSAIAVGDPLTPDGTGSPSGTAANDGVFVKGT
                     VGTHHIRAIALEALIAANTKIKIDLIG"
     gene            15279..16226
                     /locus_tag="PLQ68_04555"
     CDS             15279..16226
                     /locus_tag="PLQ68_04555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK71957.1"
                     /translation="MPHWSDVVSSVFVRNVANRYVVDNPLEAFDIFPDVPTTKLAGYL
                     AKYKKADWFRIGTKNDYKRTGATESFGDDYEVDKQSYLVEEFAFHKDITEDDRNEYDN
                     PYDPVKDAISFCDNRIRRVLLQNLVDTYIGTGIWGTDLDLSGSSYNQWDAKTSGVSDA
                     NPVEYILDAKEAIKKTTGYEPNKLIITYDVFKALKTNTHITGSIKTTNDKVVTKDLIA
                     RLFELDDIRILDAVNSDADDFLVSGTALLVHTPKKPSKFEPSAGYHMVYKYRGKDKIR
                     TQKILMEEKNNALRIELAVKVDPLVVASDLGYYMYNVVS"
     gene            16294..16623
                     /locus_tag="PLQ68_04560"
     CDS             16294..16623
                     /locus_tag="PLQ68_04560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK71958.1"
                     /translation="MKEDILLLEVGSEALDELTDKEKNLILCKFLDSQIKLAGMFVFN
                     LLRKKYRPSYRMGSMYENDSAKFEKYNELFKEYAQSVSAGKLAVETNTQTTYEIERYK
                     WPEQTRQ"
     gene            16599..16913
                     /locus_tag="PLQ68_04565"
     CDS             16599..16913
                     /locus_tag="PLQ68_04565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK71959.1"
                     /translation="MARTNSTITISQKTVDWKKTDSTPISLGSYSVYLEHHISFTKLQ
                     SLFGGSFDIINKAKGFCIMFDEVDLVNCYITYNGTTYEILKYSVFNNMKGDFHHIEFI
                     YG"
     gene            16906..17394
                     /locus_tag="PLQ68_04570"
     CDS             16906..17394
                     /locus_tag="PLQ68_04570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK71960.1"
                     /translation="MAKPYTELTKDFRAFIIDNKRREAEEMVSEVIAGILPFVPIDTG
                     RLVNSGFGYVDGDLVVNTNHGTSTSKLPIIPPEPPEVVVDDPASFQGNIIFTTPKPAT
                     RGATVFYVEGGQMLFDYAPFVMGGLANIQQIITPSIINILLLKAAKNAAERIKKKWRR
                     SA"
     gene            17376..17735
                     /locus_tag="PLQ68_04575"
     CDS             17376..17735
                     /locus_tag="PLQ68_04575"
                     /inference="COORDINATES: protein motif:HMM:NF024103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HXK71961.1"
                     /translation="MAEISIAQYIAANTDLVLGADLFLAYLPEDTSNGVVVRTLFSDD
                     NSDGLAIHYIFVIIIYENYEDAKTLANSIKTLMKNHRGTTGSTWSVKDKVVSRWLGQD
                     NIKRNIFEVSFQIVDEE"
     gene            17750..18277
                     /locus_tag="PLQ68_04580"
     CDS             17750..18277
                     /locus_tag="PLQ68_04580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK71962.1"
                     /translation="MGYELGPCQILYGDVGTSEGGLTDLGKTLGGVNFTVEETFQELR
                     TDQDGESPVGETITGTKVKIAANLTDITLANLAFILKSTVITDGAKQKVLVSPNSGYS
                     LLDNAKKVVLKPYVDGVASADANDWITLTKAGIRAAIDLAYNSNDQRVIAFEATGYVD
                     DNDTIAVLGDETASA"
     gene            18281..18673
                     /locus_tag="PLQ68_04585"
     CDS             18281..18673
                     /locus_tag="PLQ68_04585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK71963.1"
                     /translation="MAIFNFGPATITHNSVNLGKTVGGGSLSLDVHSYVKLRTKEKVD
                     IVYGGSGQLNLFSWDTNIQLTGDITLLNYAKTVITCTDMIITLYNNKFTINPAIMFGT
                     YEQRPFTLDFSFMADDSSFARIIDITNV"
     gene            18689..18994
                     /locus_tag="PLQ68_04590"
     CDS             18689..18994
                     /locus_tag="PLQ68_04590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK71964.1"
                     /translation="MAKKIVNIDEFLDENSIEFTLNGKKYLVKDVDVSFEAMDMENTD
                     KREILANVLQCDVKELEKVGLLAITKVLSVITENFSQGVSQVQLSPESNEQGPSHTS"
     gene            19218..22442
                     /locus_tag="PLQ68_04595"
     CDS             19218..22442
                     /locus_tag="PLQ68_04595"
                     /inference="COORDINATES: protein motif:HMM:NF021251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure C-terminal
                     domain-containing protein"
                     /protein_id="HXK71965.1"
                     /translation="MPKYEIDFDANFEELIKQVNKIEKAVGKLVKKKPFEGMKEEADT
                     LTRKLTQLRKALNINMAAKGASLRELTRLSVDETEELGRVTKQTANEMKALDKDMTFM
                     FDAKKKSDQLIDKLGNTTAQLADKTKKAGQSVSHFTKETKKFKPQGEAYAGVLNSWWQ
                     TFGRVAIGFTIAYRAMNAFEALLSKTSETIKQAILDAGELAALQAKLAMFANMASNGT
                     ITFEEGFANASAVVNGLADASIKSISSMKELSVAMDEVAQQGVIIPAKMTESFAYFVD
                     YTLLVARTTGDSAKQLRSEITGLMDGQMRASNVLLRTMKNMGILSTKEIKDLRNMTNR
                     AEIFNKVVEAIASNQKKVNRLMIEQDPTTGYAVWEKSIRRVLTVSIQLVSQLKETQNI
                     FGEVFLKHIEKWNEEFSNIANNKDMGRFMVMMDKLAGALDIVLTAFERVVKAVAALFT
                     AFDNLSGPIKIVLKYFLYTEVFFLAVKATKLLTAAIKALIVSQTIAIGKFALTAIKLG
                     AIVIAAGLVIGTFYAIVTAIRVLIPSIKEFIQNIKDLIKDIAEFFSPVSKFFDKLHHK
                     IKEILEATKKAGKDLRKQSEENHKGFKQQFEGNQLKPGFISKLIDKEPLQGAEDSIEH
                     WRKVNEKAIEDLKADINSMFFIDPPKPTFADTFKETMKEDFAKIRAFIASVSEEAAQM
                     FDKLFNPKYLPGSTSTDGKDSVIVNPVKELNLQLEKAEGNTKDLFTQLTKLVALDTDY
                     DFDIGLTAAMTEVETKIKDLELDLKNAFASFKYALSKGLDDESNDLLVYIAKLQAELD
                     ELKKDLKDMPDTLKTAFNTQYLKDFKEKKEQIEFSNRYDPYAKAKEMRALMNEYNAKI
                     AGYVPETKEGLKALTDMKKELSATATEATDFKSSIAGAFKSFAEYNPFEGFKDAVGDA
                     LGSMEDALIDFTDTGKMAWRDMIDAMMKDLLRLVIRMAILQPIAASITSGLSGLSFAE
                     GGTISEHVVGVGLSSGRRYDIGEAGENEEVIPHSKLKGGKGDVTVNVYNAPSTPETNV
                     STDKQGNTRIDLMFDNMIGQSAVDGSLGKVLKNMYGLKPRLTGR"
     gene            22444..22794
                     /locus_tag="PLQ68_04600"
     CDS             22444..22794
                     /locus_tag="PLQ68_04600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK71966.1"
                     /translation="MASWNAGLPSEFMEEGYDETLPDGTIRFGNDIGPASVRRVTSAN
                     ITKIKGQMLLTSYQTSLMTTQYQTTCAFGATAVTWVHPRTGVVADLRFTAPPHYTPAG
                     GNYYIAQLEMEILP"
     gene            22791..23264
                     /locus_tag="PLQ68_04605"
     CDS             22791..23264
                     /locus_tag="PLQ68_04605"
                     /inference="COORDINATES: protein motif:HMM:NF020449.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1833 family protein"
                     /protein_id="HXK71967.1"
                     /translation="MSRETSLTFRQAVNSQGSDEIFILLITLDHETLEDSIRICSGGQ
                     NIVHLGDTYIYYPFDMKIPDDTADNVPQATLIIENITPTIITALRSISSEISVNIKLI
                     LASDPDTIEIEFTDMFISHISYNALTIEATIKAENYLTSYLVKDSFVPSQFPGLF"
     gene            23264..23668
                     /locus_tag="PLQ68_04610"
     CDS             23264..23668
                     /locus_tag="PLQ68_04610"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HXK71968.1"
                     /translation="MFNKYIGIPYKDKGRDFNGCDCWGIVQLVYKHELNILLPSYIED
                     YASIEELKEIELLIKDQLINWQQVDKPITFDIIIMNIIGSPVHTGVIINNTYMIHTLP
                     GHDSVCDKYYDPRWKKRIQGFYRYKEILSGKQ"
     gene            23655..>27474
                     /locus_tag="PLQ68_04615"
     CDS             23655..>27474
                     /locus_tag="PLQ68_04615"
                     /inference="COORDINATES: protein motif:HMM:NF040662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity factor TipJ family phage tail
                     protein"
                     /protein_id="HXK71969.1"
                     /translation="MENNKVLITASPHPFYHEPIITSALPNQILADVLDKSGITFYAY
                     SDLRVLVNGILITKEQYETLIVKENDIITVKAVPHGGGGKNILRVVAMIAITAITFGA
                     GSFLGLGYLTAYGLGLEALGSVGFQIATFAFSAAWFAGGMSLINAICPVKTPTEGLTN
                     AYDGKTSQSYALSMPSNKSNIWGPVPLLLGHHRFYPPYAAKPYTSVDGENQFLHLLFC
                     AGYNNVTLDEFKLGETDITEYMDTDDLDRLEMKVHYGFDPSIDEFELVKDNVDETPLS
                     IELLETESWNLRTTEEDVTEISVDITAPKGMYAINEMSGAKESRTVNFEIQYAPTGTS
                     DWSIASAGEAFLARNADVSQPTVSYTVDMYGNLEETTGYSYTRIGLDKATGEIKTTEN
                     PGTNSIANAKAYCPSLGKYIAPVCGVLRNSANTDIVIDDLRTDELDRENTTDFLASVN
                     GTSGVTIAAGTLYFRPYIRGKSPDIIRRSYRFKVTSGQYDVRVRRTTADTESSKVFDE
                     IYWTSLRSYSSAPIVDITGLILIEMRIKASDTLNGSVEDFNFVYKSNILDWDSTGETW
                     VTRGTNNPASIIRELLQGSFNANPRTNAQINLTELQEFHEYCADNSFSFNMPIDYNIS
                     LKEALDLVASVGRASHTTKNGKHTVVVDRVKDNIIQHFTARNTRNFSAVKTFTQLPHA
                     YRIVFADENSSWNTTEFLVYADGYGTGSGDTPATYIQTLDLKGITNSNLIWKQGRFHL
                     AQQQLRPETYSIETDIDNIICTRGDRVKVQHYAPLWGLGTGRVKSVTIDGSYTTAITV
                     DEIFTTEAETDYAIRCRLADGTSVYSAVTCEVGEISELTFESPILTANAPAVGDLVSF
                     GELGYEMVDCIVLTIKPGDNLTAVLELVDYSPAIFDADTGEIPEFDPFISSPVEPSTP
                     TISYVRSDDNALYRSSDGTLIPRIMAYLQAPAGIDMHKINAIQGQIREYSAVEVYDHP
                     WENLPSVTKDTREISIMPVEVGHTYRLRFRYLFNDGTTSSWCTENIHTVVGATGAPSD
                     PTFDDGATVFEYSYIKFVINGVADLDLQYYEIRRDTNFGTIDSNFVGRTNSETLMYTG
                     PITATDTYYIKARNTAGVWSANSDSIASVFDAISIENFDGDFYTNECHLTWKAVAKDS
                     IDHYVLNFYSEIERSNLIGTSGEIYTTHWILTETANYTMNSGYNRNVYATLSAVDSLG
                     RTVTSDLDISNDVPNAPSTPTLTPGLRTLTVRWTPIADVDIISYDVAFDTNADPTTIY
                     NTTNTQITFTN"
BASE COUNT         9717 a         3826 c         5397 g         8534 t
ORIGIN      
        1 gggtttattt ctgtgcggga gatggcaatg gaagatggag ctaagctcaa aggcccgttg
       61 aggccggagg ggtgaagaat gagcacaaaa atatataatg cttatagggt taagggtatg
      121 tctcttgagg aattgtttat gtttcttgtt gcgttgcgta agcaatatca caaagagtgt
      181 ataaaggaga ttaaggcttg ttatggtgat aacctaccta aatggtttga agtgcagaaa
      241 gagttggaaa gacaattaag ttctggaact aatgatgcat taaattttca ggcttctgcc
      301 gttatttatc catataaaaa acgagtctac attcagtttt ttggactaag ggagcgttat
      361 ttggagaagg ttttggatgg tagatttgaa gattatcatt accaaaacca ggcggattgg
      421 cctgaagaaa tttctgaaaa acagtggaac cggagagggc tggtatggga taaaatactt
      481 agtctgggcc aagatggtat tccaagtgat aacggcttta tatttccgat ttatgaaaag
      541 caacactgcc tctatattgc atatagtatt ggccaacttg aaaggccgga ggggtgaagg
      601 gatggaagga ccgaggagga tttacatagc tccagacgta gcgcttatgc gtatgttcca
      661 tgagcaagaa aacgaggatg aagtcgaata catccgcgcc gataaatggg gaaggagaga
      721 tctgtaatgg aagttactag cgacaaaaga cttacaattt ttatcgagct taacgagaac
      781 gaggcagact ggcttatgac ctttattcag aatcatcctg atcttgagca tgaaaatcag
      841 acggctaggg atatacgtat taagttattt aatgagttac gtatgcattt ggataaattt
      901 cactgaataa aataatttta atagatatga ccgaattttt ggttacgttg cgtatatata
      961 attaacaata aaaatgcata cattcatgta tgaaagaaaa acaaatcaat taggaggctt
     1021 tatgagagaa acaactgaag tagtcaaatc ggcagatggt ggacagtggg aatttaagat
     1081 gacgttgcct gaaacaatag atgaggcatg tgaaatcttt ggcaaggatg gtgcgcttga
     1141 tatctttaat agtggactca aagtaaagaa acagaatatt gctcgtgaag gtttcaagaa
     1201 tggcaaagat attgatgaag ttgagtcttt gtgtgaggct tataggcctg gagaaaagaa
     1261 gaagaatcag ggcgagatat ttactgaact tgttatggag aatgctgaac agcttcgtat
     1321 ggaccccgat cttaagaaag agatccttga tctttacaag acaggtaagg taaaggacgc
     1381 aatcgacaga cttcgtactc tttcagaaga ttaatagcaa gttacagtaa attaaccagt
     1441 gtgggctaga atcatccgct agcccacaca tttaggagcc taatatgaat gcctttgaag
     1501 cacttttaca tggaaaagat attacaacat taacagaaga agaaataaat gatttaatgg
     1561 ctcaaatgtc ttctgatgaa ttggctaagt ttgaacaggt tgttaaggca aagacgccaa
     1621 gaagagtgaa agaatctaaa acatcaaaga aagcggagga ctatcttaat agtcttataa
     1681 ccaagggatt atctaagaaa ggataattat gcttcttcga tcatcgtcat tctcaacttt
     1741 taaagtttgt ccgagaaaag catactatgc ttatatatta ggactagtta aaaagaatgg
     1801 ttttaacatt gatttatttt ttggttctat aatacatgat gcagtagatg tttttaataa
     1861 aacaggagat atagaagatg cgcttaattt attagaacaa tcagattggt ttcattatca
     1921 cagcaaaaag aacaaatttg ttgctttaac tttgcttaaa ttatatgcaa gatttatttc
     1981 aggacatagc attgaaacat cagaagagga atttactttt gatgtaggta atcatatatg
     2041 gaaaggaaaa tttgatagtt tatataagcc tagtaacagt acatatgtag gagaggttaa
     2101 aacaacaaat aggtggtttt tgcatacaga ccctaacgat caatttataa gttattatat
     2161 tggagcaaaa cgaaaatatc ctcgtcttca aggtgtatat atttttaatt ttgaccctga
     2221 aaaagtagat gttggaggaa caatatttgt gccaagtcaa tttaaaattg atgaatggct
     2281 agaggaaacc aaagtagcag cagactactt tcaattgtgc attgataaag aaacattccc
     2341 taaaaatccg gcaggttgct ttatatatgg aaagcaatat ccttgtgatt ttcttgatct
     2401 atgtacttct aatgattatg gtacaaggca agctattatc aaaaataact ttgttgtaaa
     2461 tgaagaagcg gtgaatctgt catggtagtt aaatgtcctg tttgtggtag taatatgata
     2521 caaagtaaat ctaaatataa tatatgtgtt cttgattcta atgaaaaaat atatgaattg
     2581 aaagatgtag aaatgcctgt acttacttgt tctgattgca ttgaaacttt tataacactt
     2641 aaggttatag cggaaaacga tacaataacg ctgaaaggag aagattatgg gaagttttaa
     2701 agatgaagcc gtaggaaaat ttatggtttt gttttggggt ccacctaagg ttggtaaatc
     2761 agtactagct tcacagtttc ctaatctgta ttatgtcaat gttgatgatg gacttggcag
     2821 tgttaaagca atgacaacaa aacaaggcat tgatattgat tttaaacact ttaatgttgg
     2881 gtcaggaaaa actacagata aagattatat agaactttgt ggagagaaaa tggcaatgaa
     2941 cagttctgcc tggacaaaga acaaaaaact cgctgaaatg ttagctaaaa catgcaaaga
     3001 ggatgataca atagttatag atcatctagc tagaataggt gagtttttac aaagacatat
     3061 tgaacgatca acaggaaata ggccattaca aatccaggat tggaatacct ttacaatgga
     3121 actgactgat tattttgact attttaaaga tgaagcgtgt aaggcaaatg ttataatatt
     3181 aggtcacgat aattattatg aagaaaaagc tacaggggaa ttgagaaaac ttttgttgct
     3241 tccatcttcg atgcgtcaaa ggattcctgg atttgtttca gaagtactgt atatacatac
     3301 agaagttagt gggggcaaaa gaaatagaca gtttactagg catttacagt caatgcctga
     3361 tcgtatgact gctacaggtt cacgttcttt gattcctgac atggaaaatc ctacttactt
     3421 gaaaatgaaa ccttatttag aagcatattt aggaaagaag ttgccagaac ctacatggac
     3481 acctgatgaa aaattgtttg gcgaaggtat agtggaaaaa gaagtaaaaa atataactaa
     3541 ataacaggag gaaagtttta tgccacagat tagtttggat tatgatcttg aaaatgtatc
     3601 atcggagttt actccccttg agccgggcac ttattatgga cgtattgagt ctgtcgagct
     3661 tacaaagagc cagaagacca ataagcctat gcttaagatt gtctggatta ttactgaagg
     3721 tgagtatgag ggacggaaac tgtttgataa tgtactgctt gaaactgagt ggaaggtaaa
     3781 gcagtatgca cttctcgctg gtattccttc gggaactgaa ctggatacag atgattttaa
     3841 ggattgtgaa gcaattctgc atgttatcca gaaacctaat cctcagggtg gtgaaggcga
     3901 gatgacaaac aatattaaaa agatcgaagc cattagttaa tccctttcag tcagggatac
     3961 ctctgcatag tgtaggggta tccctatttt attatttgga gcccttatga agatacctgt
     4021 aagtgatata attattgaaa gtgactttag ggaagataga gtagatatag gcgatttagc
     4081 cttatctatt atgaacaatg ggttgttaag tccgattata ttaacgaatg cagttgatgg
     4141 aaaatatcaa gtaatagctg gaagaaggcg ctttattgct ttacgagatt acataaatct
     4201 tgaagaatta gaagaaaatg aacactttgt ttttagagat aaagtagacc cattagtggt
     4261 tcaatttgaa gaaaatttta gacgtcttga ttttacccct attgaaacag caagattggt
     4321 aagtgctatt cataagaagt atgtcgatat acatggagaa gcagttaaag gcattaaagg
     4381 taaaggttgg agtatggatg ataccggacg ccttttgtct aaagataaaa gttttgtaag
     4441 tcgtatgtta ttatttttaa ggcatgaaga agatataaag aattgtacaa atatacgtga
     4501 tgctattaca accatagaaa agatgagaag taaaaatatc actgatatta ttcagaagac
     4561 tagggttgaa aaagcattag aagaaaagat aacaggaaag attgaagatt atatttccaa
     4621 tctatatcta ggggatgcgt tagactttct acctacagta aagaaagaat ctgtcgattt
     4681 cattctgact gatccaccat tcgctattaa ttatgatgag attgctggag gtgaaagttt
     4741 cactacttat gatgatgatc cagaggaaat attaaaattg attgattctt gcatgtcaca
     4801 ctattataga atcttgaaga aaaatagatt tattgtagtg tgggtcgctt ttgaacatat
     4861 gaatactatt ctagatatga tggacaccgc aggattttat acatctgcta ctcctttggt
     4921 atgggtaaag ttaaatgcat caggtagatc gtcaaatcca gataaaaatc ttggtagtgc
     4981 ttgtgaaatt gctatatatg gctggaaagg gacaaaagcc gaacttgtaa ctaaaggtcg
     5041 aggcaatgtc tttccatatg atactgtacg taaaaacaga atacatatag cacaaaaacc
     5101 tgaatcatta ctatgtgata tacttaatat attctctaac ccaggagata ctgttgtaga
     5161 tacttttgta ggtagtggtt ctactttaag atcttgtttt ttagctgata ggttttttag
     5221 aggttgtgaa aaacttgaag taaattacaa tagcactatt gtatatacaa gagactgggc
     5281 aaaaacgata aacaagtaag ttgcacaaaa caataaagta aaccttattt gttaatcatt
     5341 tttattggct taatggtatg catggatcat gcatgggtaa tggttttata tggtgtatga
     5401 tatacatttc gatatggtgc atatgacata tgaatcctat atataaataa aggtataaac
     5461 acaatgacat taatccaata tattcctaaa gaatcatcga aaatatgttt tgttggaaaa
     5521 gcaccaggcg aagttgaaat gcgtactagg gttccgttct ctggtccaga aggattcctg
     5581 tttaataaac tattacgttt agcaggaata gaaagaacat ctgtatatat tactaatact
     5641 gttcatgtaa gacctactaa aaatatttat gaaacacttt ccaaacaaac aatagaagat
     5701 ggaaaagatc aattaatatc tgatttatct gcatggaaag aaaaaggatt aactacagta
     5761 attgctgttg gagggagagc attagaaatg ctcacaggtg cttctagtgt tataaaatat
     5821 agaggtacgg cttttccatg cgctattgtc gagggattaa aagtttatgg tacgcttcat
     5881 ccagaagatc ttattcgtgg taacggcttg tatgaaccta ttgtagtggc cgacataaag
     5941 aaagcagttg cagactcaga aacatctgaa gttttttatc cagaacgtaa tatagaaata
     6001 ttacactttc cagaacaagc aataaataaa ctagaatctg taacagccat agaaaaacca
     6061 gttactgttg atattgaaac aagtgggcct gttatgactt cttatggagt agggcttagt
     6121 gctaataatg cttttgttat accttatgac ttattaaaac ttcctgcttt gcttaaagca
     6181 ataggtaaat tcgctatgtc gaatactcca aagatatttc ataacgcatt gtttgatgta
     6241 ttccataatg cttactatta taagatatta aatcgtaata tttattttga tacaatgctt
     6301 gctcaacatg cagtttatcc tacatatcca aaatctttgg ctttttgtgg ctcaatatat
     6361 actaaagaac cttattggaa agacgaagga aaatcagtat tttctcagtt atcatctaaa
     6421 gacgctttaa tgaatagtag tctctgggat actctttata tttacaatgg caaagattgt
     6481 tgccttactt atgaagtata tgaagcattg ctaacagaat tagaggattg gaaagtccgt
     6541 cctactttcg agttatctat ggaattactt cagccttgtt tgtatgctat gcttactgga
     6601 cttagagtgg atgaattagc agttcaagaa tttgcagaca aaaatgaaaa agccatagaa
     6661 atcatggaaa gaataaaaga acatgcaata ggaccgatta atattaaatc acataaacaa
     6721 cttaaagaat taatttatga tacttggaga atgcctgtac agaaaaaaca aggaaaaata
     6781 acaacagaag ataaaaaact tgctagactt gcatcattcc caactccata taaagcacgt
     6841 atagaattaa tccgaaaact taaggaatca tataaaaaac gtgactttta tacactaaaa
     6901 ttagacacag atggacgtat tcgatatgca ttaaatatag gtggaactta tacaggtcgt
     6961 atgtcatctt caaagagtat aactggaagt ggaatgaatt ttcaaaacca gccgaaagaa
     7021 gttcgttctt tctatatagc tgatcctgga gagatatttg tacaagtgga tttaagtcag
     7081 gctgaggcac gagttgttgc tgctctttgt aaagatatag agtggttaga agcctttgat
     7141 aaagaagatt tacatagtaa aactgcttca tttttatata acattcctat agagaaagta
     7201 gaaaagaaaa ctcatcgaca agtcgctaaa cgtgtagctc atggaactca ttatggtttg
     7261 ggtaagatgt tattgagtga aatattagag tgttcaatgg gagaagcgga aaggttaaaa
     7321 aataagtatc ataatatgca tcctaaactg catgaatggc aagaacgagt acataaaact
     7381 gtaaaagata aaaaggttat tgttacacct tttggtcgag taatgcagtt tttcgggcct
     7441 ttgaatgata gtcgattaag ggaagctgtt gctgctgaac cgcaatcaat atctgctgag
     7501 tatcttaata gaggattaat acgttgttgg aaaacgatac ctaattttaa gtttaaacta
     7561 caggttcatg actcaattct tgttgctgta aaagatgata taaatataat taactatacg
     7621 atgttaaaaa tgaagcgttt aattgaacaa aaaatagatg tgcatggaat tgaaatgatt
     7681 atcccctgtg attttgaaat aggttattcg tggggaaaga tggtcgaagt taaagacctt
     7741 gctaaagtta aggagatata tgagacatta cagaaaaagg atatgtgatg aaatacaatc
     7801 gcttcttaga atgctatcgg ttttatactc gtggtaatga aacgccagaa ttatttcatt
     7861 tatggtgtgg tctttcagct ttggcaggtg cttgtgaaaa aagattatgg ctagatagag
     7921 gattctttaa tgtttatttt aatctttatg tagtattaat tagtcctgct ggactatgtg
     7981 ctaaatcttc atcacttgat cttgcaggaa agctacttaa agatgcaggt tatcgtttac
     8041 tggaaggttc agttctaaag gaaaagataa ttattgagat gctagaaagt atgaaaacag
     8101 tccaattaga cgtagaaagt atttttcctc attgcagtgt aacttattta agtgatgagt
     8161 taaatgtact tttatcttct ggtgtcgata tgattaagtt tctagtcgat atgtatagta
     8221 aggatgatct gtatgcttat aaaacaaaaa attctggtac tttcgagttg ccataccctt
     8281 actttaattt attaacagct gctgtgcctc aatggtttgg gcctcagtta gctggtgata
     8341 tggcatctac aggttttctt gctaggtgca tattaatata tttagaccaa aaaagaggaa
     8401 agtatcctca accaatttat actaaagaac aagtagatgc aagagcagag tgcttagaga
     8461 tgttgacgtc tattgctgat tggtatggcc cacttgaatt atctgaatcc gctaaaaagt
     8521 tttatgatga ttggtatatg tcgcaagata ttagtccatc agaagattac cgtatagcag
     8581 cttatcttga acgtaggaat aaagtacata tattaaaagt agccggttta atggctgttg
     8641 gagatttaag aaaaataata caaaagattg attttgaacg tgccattgat ctatttaaaa
     8701 tgactgagaa aagattgcgt atgtcatatc ttattgcagg tggtaacaaa ttagcacaat
     8761 acgtttataa aatacaagga ctattaaaag ataatggtgg caagctatac acaaaggatt
     8821 tgatgaaaat gttttataca gatttgaatc ctgccgattt taagatatgt ttgcaaacac
     8881 tggttgaaat gaacgagatt gaattagtac aagaaaaaag cactaaattg tattatataa
     8941 acaagttgaa aggagaaaat tgatatggga tttataggtt ttagaacgag tgataaacta
     9001 caacagttga ttgagaaggc tgcaaaaacc agaggactta gtgtatctac attgtgtaga
     9061 gatataattc gtgataatct tgatactacg atgggctact ctaattggaa agctatagca
     9121 tggaatttat ttaatataac tgctgaccag cttaattatg ctctgaataa agctacttgt
     9181 cctgatgatt tcttttcgat agtatcaaag tggtcaaaag ttactaagat acagataatt
     9241 ccaggaaaag aaaatgtaac tttaaagttt gaacatcaag gagaggctta tataattcga
     9301 gagcctagaa atatgcctaa acttgtaact attgcaaata taattgaatt acttgataca
     9361 aagatttacg aaacttatat gaaaggagaa gcagatggaa acaagaaaga taacaattaa
     9421 tatttcagaa gagcaggcaa aacatcttga taaattagtt gaattactta atagtgatta
     9481 tagtaaaata ataagaaata tgttagatga ttatgctgtt aactggaata tagataaaaa
     9541 atttgcagaa gattgtcagt tagcttctat atatttcctt gattttacta tatatttgaa
     9601 atgtcgtatg gttacagaga taataaaaaa gaatagtaat tataaagtag atccatacgt
     9661 agaaagatca ttgcatgaaa aattagaaag ccttaaaaca gtatttagta ctaatagaga
     9721 ttttttatct tttatttcat ggtatagatt gctattaaaa acagcagaat aaaaagatga
     9781 atagaaaatt tatatatgtt acaggtccat ttgaagggct taatgaatat gctaagaaag
     9841 caaatataac acaacttgat ttagtttgtc gccgtgttat tgtcgctaag catataccat
     9901 tatgccctat tcttttttat ggtgcttgga tgaaagaccc tcgattttac tcacaaagtg
     9961 cttggtgggt agacaatata tttaaaccta ttatgttgca gtgcaattat ttttgctata
    10021 taccaccgta tgcaggtgta cgtaatgaac gacttgaata tgaaaaagat atatggaaaa
    10081 cactgaatag aactagaatg attatagcag acagacttat aaaatacttt aacgaggatg
    10141 tagataatgg acataactaa agttaatagt tttgaacgac ttgaagaggc ttttttggat
    10201 gaactatttg tgcgttatat tgctaggcgt tttttagatt taagttcttt acgtgctgtt
    10261 gcagttgaac ttgatttgaa tgtaaatagc tttgatagat tatttactgt tgatcctaaa
    10321 catgagaata tatttaataa atatttagat gaagaaatag ataaagcgga tatattatta
    10381 tcaaaagcta gtaaaataat agcaagacag agattatttg atatattaag agaacatgct
    10441 ggtgcggata ataaagaagt tatacaagca gctaaagcca ttcttacatc tgttaatgtt
    10501 aaagatggtg gtaagtccga tggatttgac aaaattttag aagacatgat aactagggag
    10561 gaatgaagga acaacgacag cgaaagataa gacaaatagt aaaaatactt aatgctaagt
    10621 atgcagaaga ttttcatggt tgggtcagag ataatattga tttttcaggg cttgattttg
    10681 atgatcttac atggcaacaa atagaaatag gcaatgcaat aattcatgat aaaaatgttt
    10741 gtgtggcagg cggtggtggc ataggaaaga ctgctgtcgc tgctttgctt attctttggt
    10801 ttttatcttc ttttctcgat gcaaaaattc ctaccacagc accttctggt aagctccttg
    10861 atgatattct atggagtgag ataagtacat ggcttaatag gtgcaaatta aagcatttat
    10921 ttgagcttaa atctggtaaa ttacaaataa aagaatttaa agaatggtat gctgttgcgc
    10981 gtactgttcc tagagatggt aaacaactta atgatacctt agctggtttt catgctccta
    11041 atcttcttat catggttgat gaatcttccg gtgtacctaa tcctgtattc acagcacttg
    11101 atggtgcaat gaccaaggaa aatgctcgta tccttcttat ttctaatcct gtatcaactg
    11161 gaggttatta ctatgatact attagtgatc ctaaaggtaa aggtaaagga tttaaagtgt
    11221 tatttttcga tgcaagagaa agtccattag tatcaaaaac atacgaagaa cgtattatag
    11281 ctcgttatgg tcaagattca cctatgtata aagctaaagt gcttggcctt ccaatagacc
    11341 tagaagatac acttcttatt agcccacgac agtttgatga ccttatagct cgtaataaag
    11401 atagagctta tggcagagtt gtgcttgggg tcgatgtcgc aggtcgaggc acagatatgt
    11461 cagttatatg taatcgtcat ggacagtcta tcgtagcatg ggacgaattt aatactaatg
    11521 atactgtatt attaaaaaat agtatatatc gtatcattga atctaggtat aaaaattatg
    11581 atgtagttgt tgtagtcgat ggtgtaggta ttggttctgg tttatgtgac ttattagaaa
    11641 gtgaaagacg atataaaata atacgtttta taggatcaga aaaagctcat gatactaata
    11701 tgtatgatag aaagcgtacc gaaggttatt acacacttaa aaaagtcata atggatactc
    11761 attttcctgt aaaacctcct gaaagactta aaaaagaatt agtcaatatt aaatttaatt
    11821 atgataaagg cccaatatct atgcaagcaa aagagaaact tatagatata cttgggtttt
    11881 ctcccgacta tgctgatgct ttgatgttaa gtagtgttgt agataatttt agtgttggct
    11941 ctgttgggct ttttatgcca caatcaatta ataatcggtt gtcgaaaata ttggttacac
    12001 aaaaacaagc taatagattc gggaaatata gtagattcct tcaataatag gagaagatta
    12061 tgtttggtaa taacaagtta caaaaagaga tagaccaaat caaacatgaa ctatttgaag
    12121 taaagaaagc taatgatgct tttgtatcta gagctaaaat gggcagttta ggtacagaag
    12181 attttatgaa gggaaccagt aaccttgaat ttctaacaga attagagcct cctacaggat
    12241 tacagttata ttataagatg tatcgcaatg agcctataat tggtggtatc atgttactac
    12301 tttataatat actgtctggt ttatcctggg aattaactgg aagtaataaa gcatttataa
    12361 aggatatgtt tgataacttt cctgttgata tgtcctctat atttttcgat atggctagtg
    12421 cattatgttt tgggttttat gtaggagaaa tgcttataac agttgataat ggaagaatta
    12481 ttctggttga tattgaacca agatttcaaa caactatcga gaaaataaat aatgaagaag
    12541 gaaatgttgt acaacagtgt actgaaggtg tgtataatat tccttatact aaatgtgttc
    12601 atcatacatt tttagcatct aatagaaatc cttttggaga tagtattatt agggctgctt
    12661 ataagcctta ttattacaag ttatctgtag aagcatcaga agcaactggg cttgaaagag
    12721 atttaactgg attacctata ttaacagcac cagaaaattt tgattttaca gctgctttag
    12781 aaaccagtcc tacatatgat cctaatgcag cagaaacatt acagtgggct atttcacttg
    12841 taggagctgt acgtaaagat agtcagcaag gtatagtaaa acctagtgga tgggattttc
    12901 agattgttag aggagaaaac agaactacta ctccaactaa tgaaattatc gctaggtata
    12961 atactgaaat ggctataagt acattagcta atttcttagc tctaggtgct tttgcatcta
    13021 ccaataatgc aaatgtaggt gtgcatttaa acaattttgt aacttgttgc gaatcttacg
    13081 ctaggaaaat gtgtactact ttacagaaag tcattaatag attatgtaag tataataaca
    13141 tgacagcacc cacattaagt ctattttttc caaataagag tgctttaaaa gaagtaggtg
    13201 aatttgtaga taaattaaca aagaatcaag ttataaggcc tacagactca ttggaaaaat
    13261 ctttattaat gttagctaac ttaccatatg aaaagcaaaa aggagacaga aatgccaaac
    13321 gtgacacaga aaacacagat cagtaaagaa ttatatagag catactcatc acaagaacgt
    13381 agaagaaagt tgcacgcaga caactttcta ttaataacta aagacggtaa caggaaattt
    13441 ccggttaaga atctagatgg taagtttaat aaggaattag tagtagcagc tatagctagt
    13501 gctgaaagat ataactatac tatggtagct aaagaagcag cacgaattta tgataagtat
    13561 tttaaagatt ctgatcctac atttgatttt agtattcagg aacatgatgc agaaggtaag
    13621 gaaatctttg gtatagtact tgaacctgat tataaagatg ctgatggtca cgttttcgat
    13681 gctataacta tccaggaagc atgttatgaa tataattcaa actttagtaa cactgcttat
    13741 aggcatactg taagattaaa taaaaatgaa gtaactttag ttgaaagtta tacagcacca
    13801 actgatttta aacttaatga taaagaagtt aaaaagggaa cttggctaca acggtggctt
    13861 atacatgatg agaatatacg caaagatatc ttaactaata agattgtagg attttctata
    13921 ggtggttatc taatatctga ataaaaatca agatcatact tctccttaac tgtttttgtg
    13981 cctcatagca atatgaggca ctttttttat gtttaatgga tttattgaca aaaaatcagt
    14041 ttactatgaa aattgacatt acattattgg aggaatatat ggctaaaatg aaaatgaaat
    14101 taagagtaca cgaagtgagt ttagccgcca atccaaaaaa cagacaaaga tttattcttg
    14161 taaaggacaa aggaggtatt atgaacaaac tggcaaattt tttggcactt attgcaagca
    14221 aagctgatgt atctgaggta attaaagaag agcttaaaga tactgatact aagctgaata
    14281 aaacagatat cattgcttgc cttaaggatc ttgggatcag ctttgaagct aaagacttta
    14341 tggctgaagg ggaatctgtg gtaaaaactg ctgaagtcga gatagttaaa aaagacagtg
    14401 atatgaaggt agttgatact acaaaattcg atgttcttgc aaaagatagt tacactctta
    14461 aagaggagca taagtatgct aatcttgatc ctgaaattcg gaaggatatt gaagatagca
    14521 agaagactat cttgcagctg agaaaagatc gttttgtcaa tgatgttgtg cagaagatag
    14581 gcaaagaaca tgctgaaaag tttgaagttc tttttgatag tattgagtcg gatgcccttg
    14641 attctatcct gaatgtagtt gtatctcttc aggaaaccat caaagatctt ggaaagaagg
    14701 ttggcagtgt tgaacttgat ggtcttaatg aaaacgaagc aactattact cgagaagtcg
    14761 aaaaactagc aaaagatcag aagatttctg tttcggatgc ttgggtagag tatgctaagg
    14821 caaatccaga aaaaatgaag tccttggatc attaatagga ttttggaggt aaaatatgtc
    14881 aaaatacgag cagtatgttg agaaaagaaa cctggtctca tctacaactt tggctgacca
    14941 ggaaggtcgt gtagtaacct atgcaggggc tcttactaca cagaaaggtg atattgccgc
    15001 aggtgtagtt gaaacaggca atggtgctag tgaagcatct gttatttata gcagaggtca
    15061 ctgtgaagca tattgtgatg gtgcagtttc agctattgct gtaggtgatc ctcttacacc
    15121 tgatggaacg ggttcaccaa gtggcacagc agctaatgat ggcgtattcg ttaaaggcac
    15181 agtaggcaca catcatatta gagctattgc ccttgaagct cttatagccg caaacactaa
    15241 gattaaaatc gatcttatag gttaaaggag gtattgttat gccacattgg tcagacgttg
    15301 ttagttcagt ttttgtaaga aatgtagcga atcgctatgt tgtagataat cctctggaag
    15361 catttgatat atttcctgat gtacctacaa caaagctcgc aggttatttg gcaaagtata
    15421 aaaaggctga ttggttccgt attggaacta aaaatgatta taaacgtact ggagcaactg
    15481 agagtttcgg tgacgattat gaggttgata agcagtcgta tctggttgaa gagtttgcat
    15541 ttcataagga tataactgaa gatgatcgta atgagtatga taatccttat gatcctgtaa
    15601 aagatgctat cagcttctgt gataatcgta ttcgtagagt tcttctccag aatcttgtag
    15661 atacatatat aggaacaggt atatggggaa cagaccttga tctatctggt agttcttaca
    15721 atcagtggga tgcaaaaaca tcaggtgttt cggatgctaa tcctgttgaa tacattcttg
    15781 atgctaagga agctatcaag aagactactg gttatgaacc taataagctc attatcactt
    15841 atgatgtatt caaagctctt aaaactaata cacatatcac aggcagtatt aaaactacta
    15901 atgataaggt tgttaccaaa gatcttattg ctagattgtt cgagcttgat gatattcgta
    15961 ttctggatgc agttaatagt gatgctgatg atttccttgt atccggtaca gcacttcttg
    16021 tacacacacc aaagaagcct agtaagtttg agccttctgc aggttatcat atggtctaca
    16081 agtatagagg taaggataag ataagaacac agaaaatact catggaagag aagaataatg
    16141 ctcttcgtat tgaattggct gtaaaagttg atcctctggt agttgcctcc gatcttggtt
    16201 actatatgta caatgtagta agttaaaagt aggttattta aaatgttaga gggaatgcat
    16261 taagtattcc ctctaatttt cttaggagtt tatatgaaag aagatatttt attattagaa
    16321 gtaggttcag aagcattaga tgaactcaca gataaagaaa aaaatcttat tttgtgtaaa
    16381 ttcctagaca gtcaaattaa attggctgga atgtttgtat ttaatttact tagaaaaaaa
    16441 tatcgccctt catatcgtat gggttctatg tatgaaaatg attctgctaa atttgaaaaa
    16501 tataatgaac tttttaaaga gtatgcacaa tctgtaagtg caggaaaatt agcagtagaa
    16561 actaatactc agactactta cgaaatagag agatataaat ggcccgaaca aactcgacaa
    16621 taactatatc acagaaaaca gttgattgga aaaagactga tagtacacct atttcactag
    16681 gtagttattc tgtatattta gagcatcata taagttttac taagttacaa agtttatttg
    16741 gtggatcttt tgatattatt aataaagcta aaggcttttg tatcatgttt gatgaagtag
    16801 acttagttaa ttgttatatt acatataacg gaacaactta tgaaatactt aaatattctg
    16861 tttttaataa tatgaaaggt gattttcatc acatcgagtt tatatatggc taaaccttat
    16921 actgaactaa ctaaagattt tcgtgctttt attattgata ataaacgccg agaagcagaa
    16981 gaaatggtat ctgaagtaat tgctggcata cttccatttg tgccgataga tactggacgt
    17041 ttagtaaata gtggtttcgg ttatgttgat ggtgatttgg ttgttaatac taatcatggc
    17101 acatctacaa gtaaattacc tataatccca ccagaacctc cagaagtggt agtagatgat
    17161 ccagcatcat ttcaaggcaa tattatattt acaacaccta aaccagcaac tagaggggct
    17221 actgtttttt atgtagaagg gggacaaatg ttatttgact acgctccttt tgttatggga
    17281 ggtctagcta atatacagca gataataact ccgtctataa taaatatatt acttttaaaa
    17341 gcagctaaaa atgcagctga aaggataaag aaaaaatggc ggagatcagc atagctcaat
    17401 acatagctgc aaatactgat cttgttcttg gggcagattt atttttagct tatcttcccg
    17461 aagatacaag taatggagta gttgtacgaa cgctattttc tgatgataac tcggatggat
    17521 tggctattca ttatatattt gttattataa tatatgaaaa ttatgaagat gcaaaaacat
    17581 tagcaaatag tattaaaaca ttaatgaaaa atcatagggg aacaacagga agtacttgga
    17641 gtgttaaaga taaagttgta agtaggtggt taggtcagga taatataaaa agaaatatat
    17701 ttgaagtttc atttcaaata gtagacgaag aataaaggag gaataaaata tgggttatga
    17761 gttgggtcca tgtcagatac tttatggtga tgttggaaca agtgagggtg gtcttactga
    17821 tctgggaaaa acacttggtg gtgtaaactt tacagtagaa gagactttcc aggaacttag
    17881 aacagatcaa gacggggaat cacctgttgg tgaaacaata actgggacta aagtaaaaat
    17941 agctgctaac ttaactgata ttactttagc taatttagca tttattttaa agtctactgt
    18001 tattacagat ggtgcaaaac agaaggtttt ggtatctcct aattctggtt attctctact
    18061 tgataatgct aaaaaagttg tattaaaacc ttatgtagat ggtgtagctt ctgccgatgc
    18121 taatgattgg ataacgctta caaaagctgg tattcgtgct gctattgatc ttgcttataa
    18181 tagtaatgat cagcgtgtta ttgcttttga agctactggc tatgtggatg ataacgatac
    18241 tattgctgtt ctcggagatg aaacagcttc agcataagtt atggctatat ttaattttgg
    18301 tccagctacc ataacgcata attcagtgaa tttgggaaag actgtcggtg gaggcagtct
    18361 ttcccttgat gttcactctt atgttaaact acgtacaaaa gaaaaagtag atattgttta
    18421 tggtggatct ggacaattaa atttattcag ttgggatact aatattcagc ttactggtga
    18481 tattacttta ttaaactatg ctaaaactgt tattacatgc accgatatga ttataacttt
    18541 atataacaac aagtttacta taaatcccgc aattatgttt ggtacatatg aacaacgacc
    18601 ttttactttg gattttagct ttatggcaga tgattctagc tttgctagga ttatagatat
    18661 aactaacgta taataaagga gaagagtcat ggcaaaaaag attgtaaaca ttgatgaatt
    18721 tttagatgaa aattcgattg aatttacatt aaatggtaaa aagtatcttg tcaaagatgt
    18781 tgatgtaagt tttgaggcaa tggacatgga aaatacggat aaaagagaga ttcttgcaaa
    18841 cgtattgcaa tgcgatgtaa aagaattaga aaaagtagga ttgcttgcta ttactaaggt
    18901 tctgagtgta attactgaaa atttctccca aggagtttct caagtacagt tatctccaga
    18961 atcgaacgag cagggaccat cgcacacgtc ttaaacattg aatttgaaaa agccttaacg
    19021 cttcctactg ctaaacttat tgtttacttt aatgaagcgt taaggcaaaa agcaatagat
    19081 gctataatgt taggattatt gttcgagtca aaggataaaa aacttgagaa gctccttaaa
    19141 atacatggac attatactga aggtttttat gataatgagc gtaaaaaact taaacagtat
    19201 ttatctacga ggtagaaatg cctaagtatg aaattgactt tgatgctaat tttgaagagc
    19261 ttattaaaca agttaataag atagaaaaag ctgtcggtaa acttgttaag aaaaagccct
    19321 ttgaaggaat gaaagaagaa gcagatacat taactaggaa acttacgcaa ttaagaaaag
    19381 cattaaatat aaatatggct gctaaaggtg caagtcttcg tgaattaact aggttgtctg
    19441 tagatgaaac agaagagcta ggacgagtca ctaaacaaac agctaatgaa atgaaagcat
    19501 tagataaaga tatgactttt atgtttgatg ctaagaaaaa atctgaccaa cttatagaca
    19561 agttaggaaa tactactgca cagttagcag ataagacaaa aaaagcagga caaagtgtaa
    19621 gtcattttac aaaagaaaca aaaaagttta agcctcaagg tgaggcttat gcaggtgtgc
    19681 ttaattcttg gtggcagaca tttggtaggg ttgcaatagg ttttactata gcatatcgtg
    19741 ctatgaatgc ttttgaagca ttacttagta aaacatctga aacaataaaa caagcaattt
    19801 tagatgctgg tgaattagct gctttacagg ctaaacttgc aatgtttgct aatatggcaa
    19861 gcaatggcac tatcacattt gaagaaggtt ttgctaatgc aagtgcagtt gtaaatggct
    19921 tagctgatgc tagtattaag tccattagct ctatgaaaga gcttagtgta gctatggatg
    19981 aagtcgcaca gcaaggtgtg attattcctg ctaaaatgac tgagtcattt gcttattttg
    20041 tagactatac actacttgtg gcacgtacca ctggtgattc agctaaacag ttacgttcag
    20101 agattacagg tcttatggat ggtcagatga gagcatctaa tgttctttta cgtactatga
    20161 agaatatggg tattcttagt acaaaagaaa taaaagacct tcgtaatatg actaatcgag
    20221 ctgaaatatt taataaagtt gtagaggcta tcgcatcaaa tcagaaaaaa gttaataggc
    20281 ttatgataga acaagaccca actacaggtt atgctgtatg ggaaaaaagc ataagacgag
    20341 ttcttactgt aagtattcag ttagtaagcc aacttaaaga aacacaaaat atttttggag
    20401 aagtttttct aaaacatata gaaaaatgga atgaagaatt tagtaatata gctaataata
    20461 aagatatggg caggtttatg gttatgatgg ataaacttgc cggagctctt gatatagtat
    20521 taacagcatt tgaaagagta gtaaaagcag tagcagcatt atttactgca tttgataact
    20581 tatcaggacc aataaaaata gtattaaaat attttttata cactgaagtc tttttcttag
    20641 ctgtaaaagc tactaaatta cttacagctg caataaaggc actaattgta agtcagacta
    20701 tagcgatagg aaaatttgca ctgactgcta taaaactagg agctatagta attgcagcgg
    20761 gtttagttat tggcacattt tatgctatag ttacagcaat acgtgtttta attcctagca
    20821 ttaaagaatt tatccaaaac ataaaagacc ttataaaaga tatagcagaa tttttctctc
    20881 ctgtatctaa attctttgac aaattgcatc ataaaataaa agaaatatta gaagcaacca
    20941 aaaaagctgg aaaagattta agaaagcaaa gtgaagagaa ccataaagga tttaagcaac
    21001 aatttgaagg aaatcaacta aaaccaggtt ttataagtaa acttatagat aaagaaccat
    21061 tacaaggtgc tgaagatagc atagaacatt ggcgaaaagt taatgagaaa gctattgagg
    21121 atttaaaagc agatattaat agtatgtttt tcatagaccc gcctaagcct acttttgcag
    21181 atacatttaa agaaacaatg aaagaagatt ttgcaaaaat acgtgcattt attgctagtg
    21241 taagtgaaga agcagcacaa atgtttgata agctatttaa tccaaaatat ttgcctggaa
    21301 gcacatctac agatggcaaa gactcagtta tagttaatcc tgtaaaagaa cttaacttac
    21361 aacttgaaaa agcagaagga aacacaaaag atttatttac acagttgaca aagctagttg
    21421 ctttagatac ggattatgac ttcgatatcg gtcttaccgc tgctatgact gaagtagaaa
    21481 caaagataaa agacctagaa ttagacctta aaaatgcttt tgcatctttt aaatatgctt
    21541 taagcaaagg actagatgat gaaagcaacg atcttcttgt ttatatagct aagttacaag
    21601 cagaattaga tgaactgaaa aaagacctta aggatatgcc agatacattg aaaactgcat
    21661 ttaatacaca ataccttaaa gactttaaag aaaagaaaga acaaatagaa tttagcaatc
    21721 gttatgatcc ttatgccaaa gcaaaagaaa tgcgagcatt aatgaatgaa tataatgcaa
    21781 aaattgcagg atatgtacct gaaacaaaag aaggacttaa agctcttaca gatatgaaaa
    21841 aagaactttc tgcaactgct actgaagcta cagattttaa atctagtatt gcaggagcat
    21901 ttaagtcatt tgcagaatat aatccttttg aagggtttaa agatgctgtt ggtgatgctt
    21961 taggttctat ggaagatgct ttaatagatt ttactgatac aggtaagatg gcatggagag
    22021 atatgataga tgccatgatg aaagatttac ttagacttgt tattcgtatg gctatacttc
    22081 aaccaatagc agcatcaata acttcaggtt taagtggatt aagtttcgct gaaggtggaa
    22141 ctataagtga gcatgttgtt ggtgttggtc tttcgtctgg tcgccgctat gatataggag
    22201 aagcaggaga aaatgaagaa gtaattccgc attcaaaact taaaggtggt aaaggggatg
    22261 taactgtaaa tgtatataat gctccttcta caccagaaac aaatgtatct acagataaac
    22321 aaggtaatac tagaatagac ctgatgttcg ataatatgat aggtcagtct gccgtagatg
    22381 gttctcttgg aaaagtatta aaaaacatgt atggtttaaa acctagactt acagggagat
    22441 aatatggcta gttggaatgc tggattacct agtgagttta tggaagaagg ttatgatgaa
    22501 acattacctg atggaacaat acgttttggt aatgatatag gaccagctag tgtccgccgt
    22561 gtaacatccg ctaatataac aaaaattaaa ggtcagatgt tacttacatc atatcagaca
    22621 agtttaatga ctacacaata ccagacaaca tgtgcttttg gtgctactgc tgtaacttgg
    22681 gtgcatcctc gtactggagt tgttgcagat ttacgtttta ctgcccctcc tcattataca
    22741 cctgctggcg gaaattatta tatagcacaa cttgaaatgg agatactacc atgagtcgtg
    22801 aaacttcttt aacttttcga caagcagtaa attcacaagg ttctgatgaa atattcattt
    22861 tacttataac tttagatcat gaaacattag aagattctat tcgtatttgt tctggaggtc
    22921 agaacattgt tcatcttggc gatacatata tttactatcc ttttgatatg aaaatacctg
    22981 atgatacagc tgacaatgta cctcaagcaa cacttataat agaaaatata actcctacta
    23041 ttataacagc acttcgtagc atatctagtg aaataagtgt taatattaaa cttattcttg
    23101 ccagtgatcc tgatactatt gaaatagaat ttacagatat gtttataagt catatatctt
    23161 ataatgctct taccatagaa gctactataa aagcagaaaa ctatttaaca tcatatttag
    23221 taaaagattc ttttgtgcct tctcaatttc caggattatt ctaatgttta ataaatatat
    23281 aggtatccct tacaaagata aaggcagaga ttttaatggg tgtgattgtt ggggtatagt
    23341 gcaacttgtt tataaacatg aactaaatat tttacttcct tcttatattg aagattatgc
    23401 atctatagaa gaattaaaag agatagaact tcttattaaa gatcaactta ttaattggca
    23461 acaagttgac aaacctatta cttttgatat aattatcatg aacataatag gtagtccagt
    23521 acatactggt gttattataa ataatactta tatgatacat acattacctg gacatgattc
    23581 agtatgtgat aaatattatg atcctagatg gaaaaagaga atacaaggat tttatcgtta
    23641 caaggagata ttaagtggaa aacaataaag ttcttattac agcttctcct catccttttt
    23701 atcatgaacc tataataaca tctgcattgc ctaatcaaat tcttgcagat gtattagata
    23761 aatcaggtat aactttttat gcatatagtg atctacgtgt tcttgttaat ggcattttaa
    23821 tcacaaaaga acagtatgaa acattaatag taaaagaaaa tgatataata acagtaaaag
    23881 ctgttcctca tggaggagga ggaaaaaaca ttttacgcgt agtagcgatg atagctatta
    23941 ctgctataac ctttggtgca ggttcatttt taggtcttgg atatttaaca gcttatggtt
    24001 taggtcttga agcacttgga agtgttggat tccaaatagc tacttttgct tttagtgctg
    24061 cctggtttgc aggaggtatg tcacttataa atgctatttg ccctgtaaaa acacccacag
    24121 aaggtttaac taacgcctat gatggaaaaa catcacaaag ttatgcttta tctatgcctt
    24181 caaacaagtc aaatatttgg ggaccagtac ctcttctttt agggcaccat agattttatc
    24241 ctccttatgc tgcaaaacca tatacatcag ttgatggaga aaaccagttc ttacatttat
    24301 tattctgtgc aggttataat aatgttacac ttgatgagtt taaattaggt gaaacagaca
    24361 taaccgaata catggataca gatgatttag atcgactcga aatgaaagta cattatggtt
    24421 ttgatccatc tatagatgaa tttgaattag taaaagataa tgtagatgaa acacctcttt
    24481 cgattgaatt actagaaaca gaaagttgga atcttcgtac tacagaagaa gatgtaactg
    24541 agataagtgt agatattaca gctcctaaag gcatgtatgc tataaatgaa atgtctggag
    24601 ctaaagaatc cagaacagta aattttgaaa tacaatatgc gcctacagga acatctgatt
    24661 ggtctatagc tagtgccgga gaagcatttc tagcacgaaa tgcagatgtg tcacagccaa
    24721 cagtaagtta tactgtagat atgtatggta atcttgaaga aacaacagga tatagttata
    24781 ctcgtattgg tttagataaa gctacaggag aaataaaaac aactgaaaat cctggcacaa
    24841 atagtattgc aaatgctaag gcatattgtc caagtttagg taaatatata gcacctgtat
    24901 gtggagtatt acgaaatagt gctaatacag atattgtaat agatgacctg cgtactgatg
    24961 aactagatag agaaaataca actgattttt tagcgtctgt aaatggaaca tcgggcgtaa
    25021 caatagcagc tggaacttta tattttaggc catatattag aggaaagagt ccagatataa
    25081 ttcgccgttc ttatagattt aaagtaacta gtggacaata tgatgtacga gtacgaagaa
    25141 ctactgctga tactgaatct tcaaaagttt ttgatgaaat ttactggaca tcacttcgtt
    25201 catattcttc tgctcctatt gtagatatta caggcttaat tctcattgaa atgcgtatta
    25261 aagcatctga taccttaaat ggatcagtag aagactttaa ctttgtatat aaatctaata
    25321 ttttagattg ggattctact ggtgaaactt gggtaacaag aggcacaaat aatccggcgt
    25381 ctattatacg ggaattacta caaggtagtt ttaatgctaa tccaagaaca aatgcacaga
    25441 taaatctaac agaactacaa gaattccatg aatattgtgc tgataatagt ttcagtttta
    25501 acatgcctat tgattataat attagtttaa aagaagcatt agatctagta gctagtgtag
    25561 gtagagcatc acatactacg aaaaatggta agcatactgt tgtagtagac agagtaaaag
    25621 ataatataat tcaacatttt acagctagaa atacacgcaa cttttcagct gtaaaaacat
    25681 tcacacaatt accacatgca tatagaatag ttttcgctga tgaaaattcc tcttggaata
    25741 caactgaatt tttagtgtat gctgatggtt atggtacagg ttcgggtgat actcctgcta
    25801 catatattca aacgctagat ttaaaaggca ttactaatag caatcttata tggaaacaag
    25861 ggcgttttca tttagctcag caacaactta gacctgaaac atactcaata gaaactgata
    25921 tagataatat aatatgcaca agaggcgata gagtaaaagt acaacactat gcacctttat
    25981 ggggtctagg tacaggacga gttaaatcag ttactataga tggatcatat actacagcta
    26041 ttacagtaga cgagatattt acaactgaag ctgaaacaga ttatgctata agatgtagac
    26101 ttgctgatgg tacttctgtt tattctgctg taacttgtga agtcggagaa ataagtgaac
    26161 ttacttttga atcacctata ttaacagcta atgccccagc agtaggtgat ctagtatctt
    26221 ttggtgaact tggatatgaa atggtagact gtatagttct taccattaaa ccaggagata
    26281 atttaacagc ggtattagaa ttagttgatt attcacctgc tatatttgat gcggatacag
    26341 gagaaatacc tgagtttgat ccttttattt ccagtcctgt agaaccgtca acacctacta
    26401 tatcatatgt gcgttcagat gataatgctc tttataggtc tagtgatggt acacttattc
    26461 ctagaataat ggcatatcta caggctcctg ccggtataga tatgcataag ataaacgcta
    26521 tacaaggaca aatacgagaa tactcagctg tagaagttta tgaccatcct tgggaaaatt
    26581 taccttctgt aactaaagat accagagaaa tttctataat gccagtagaa gttggacata
    26641 catatagatt acgttttaga tatctattca atgatggaac aactagtagt tggtgtactg
    26701 aaaatataca tacagttgta ggtgctactg gcgcaccaag tgatcctact tttgatgatg
    26761 gtgctacagt ttttgaatat tcttatatta aatttgttat taatggagta gcagaccttg
    26821 atttacaata ctatgaaata cgaagagata ctaactttgg cactattgat agtaatttcg
    26881 tcggacgcac taattcagaa actttaatgt atactggtcc tattacagca acagacacat
    26941 attatataaa agcaagaaat acagctggtg tatggtctgc taattcagat tcaattgcat
    27001 cagtattcga tgcaattagt atagaaaatt tcgatggaga cttttatact aatgaatgtc
    27061 atcttacttg gaaagcagtt gctaaagact ctatagatca ttatgtatta aatttctata
    27121 gtgaaattga acgtagtaac ttgatcggaa catcaggtga aatatataca acacactgga
    27181 ttctgacaga aacagccaat tatacaatga attcaggtta taatcgtaac gtgtatgcta
    27241 cactttcagc tgttgattct cttggaagaa cagttaccag tgatcttgat atatctaatg
    27301 atgttccaaa tgcaccaagt acacctactc ttacaccagg acttcgtaca cttactgtta
    27361 gatggactcc catagctgat gtagacatta tttcttatga tgtagcattt gatactaatg
    27421 ctgatcctac tactatatac aatactacta atacacagat tacattcact aact
//
LOCUS       DAOSXM010000021        27058 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2111
            NODE_1133_length_27058_cov_10.276377, whole genome shotgun
            sequence.
ACCESSION   DAOSXM010000021 DAOSXM010000000
VERSION     DAOSXM010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864178
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 27058)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27058)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:24:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,261
            CDSs (total)                      :: 2,229
            Genes (coding)                    :: 2,218
            CDSs (with protein)               :: 2,218
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 3 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27058
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1133_length_27058_cov_10.276377"
                     /isolate="AMR_MDS_2111"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            89..1903
                     /locus_tag="PLQ68_04620"
     CDS             89..1903
                     /locus_tag="PLQ68_04620"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXK71970.1"
                     /translation="MNKLIGKPFFFRITALVLTILIISLIFTTIIYTVASEEIFISYQ
                     TERLKEETDMLASLIPNEWTTKDLLEQFFDKVFQVESTILSSYVVISDKINVEMYSYV
                     PSSQRAGIVIDKDAMDRFYKQNLSQYFKRVLSGQYISLENKNNEFDKEMIILMRPILS
                     DTGSVEAVLTVCKFVEDYSIQLENINKTLIIAMFAVFLLMLYPVYMLSKSITKPLFEI
                     KDVAIAMGKGDFSVRANENYPAEIGQLSTTMNLLSSELDSTINHLKRETNVLQGVLNS
                     MNEGILVIDKHFNPIIINPATGKLFNTDIKSSDKLNIIPKEEIWKDFEKCIETNMSVD
                     NTYNFDEIMIQCIINPVIVNNEIVGAMGIFSDITKEVRLEQTRREYVANVSHELKTPL
                     TGVMGLIEPLKDGLVTDNDKINRYYELISNEVMRLNRLINDLLVLSRLQSSNEAFSME
                     KVNLTEIINDIVERYLFYNKDQIFNIKCNIPKEPVFILGNGDRIDQILTILVNNALKF
                     GKDDVDIALCIEISLTKENNKYIVSVADNGKGISKDDLPYIFERFFKTDKSRSKGGSG
                     LGLSIAKETLLRMNEKIWVESVESVGSVFKFTLSDYLI"
     gene            complement(1907..3112)
                     /locus_tag="PLQ68_04625"
     CDS             complement(1907..3112)
                     /locus_tag="PLQ68_04625"
                     /inference="COORDINATES: protein motif:HMM:NF014615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HXK71971.1"
                     /translation="MRLFNKNKIYLFDGSKGYMLQKYGLKTGELAEEYNISNPDIVMR
                     IYNEYCDAGSDIIQTNTLCSNRSVLERHNLSDKIYEINNTSVNLAKQAIGSRKILVAA
                     SIGPIGELLEPLGNLKFNQAYDLFVEQIDSCKEADVLNFETFTDLREMRIAIIANQDT
                     VNLPIISNMTYEKNNVTMMGHTPFICSAILKKMGADVVGVNCSNGPEKMSELLKQIAC
                     FEDFVCVKPNAGVPSFVDNKAYYSQTKEQFCSYSDDLLQYNIGITGGCCGTTPEYIKS
                     MNYVKERTRSVTVEIDSHKYIMSQYSYIDIEKPFSTYEWIIDNENMLECIDKAYDISE
                     KNVNSFVLSYKSDNADFISELIIQIQDILRKPLIFEFENEWSMNAAIRSYCGIAGVYN
                     YVHKYSVKI"
     gene            complement(3122..3661)
                     /locus_tag="PLQ68_04630"
     CDS             complement(3122..3661)
                     /locus_tag="PLQ68_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HXK71972.1"
                     /translation="MNTNKILLHTCCAPCLVYVHDRLNELSVEFDSYFYNPNIHPILE
                     YKRRLKTLISYTEQFNVKLIVDDEFLQEKWEQGFKCLDCYNIRLEKTAKYAKENGYSG
                     FSTTLLVSIYQDHEKIIEICNNLAIKYDIEFLYDDFRKGFRQGQNKARQIGLYRQKYC
                     GCINSYNSSKFKDKITWEE"
     gene            complement(3648..4649)
                     /gene="ruvB"
                     /locus_tag="PLQ68_04635"
     CDS             complement(3648..4649)
                     /gene="ruvB"
                     /locus_tag="PLQ68_04635"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018592130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HXK71973.1"
                     /translation="MEEERFVTPTFIKNDQSESGLRPKVFEEYFGQEKIKQNLKIFIE
                     AAKQRKEPIDHVLLAGPPGLGKTTLAGIIASEMGANIRITSGPAIEKAGDLAAILTNI
                     DENDILFIDEIHRLNRTVEEILYPAMEDFALDIIIGKGPSAKTIRLDLNRFTLIGATT
                     KTGMLSSPLRDRFGVSCRLEMYSTDELSMIVKRSAGILGININEYGANEIARRSRGTP
                     RIANRLLRRIRDFAQVQREDVITSEIADFGLGKLEIDKIGLDNIDRAILDTIITKFTG
                     GPVGLDTLSASINEEKETIEDVYEPYLLQLGFLKKTPKGRCVTRLAYEHLKLPYEHE"
     gene            complement(4652..5254)
                     /gene="ruvA"
                     /locus_tag="PLQ68_04640"
     CDS             complement(4652..5254)
                     /gene="ruvA"
                     /locus_tag="PLQ68_04640"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009169974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HXK71974.1"
                     /translation="MYEYIKGNLTAKKHDYVVIENNGIGYKIYTSLNTMMKVKTDEDV
                     MLYTYLHVREDAMILYGFSQEEELFLFNMLITISGIGPKAALSILSVLSYQDVATAVV
                     SDDVGLLSKAPGIGKKTAMRIILELKDKLKGIYINDTQPKGQIEQSDKYSEAVNALLV
                     LGYNSKYARQALDAVYDEKDSLQDIIKNALAGRTSSNKEM"
     gene            complement(5239..5745)
                     /gene="ruvC"
                     /locus_tag="PLQ68_04645"
     CDS             complement(5239..5745)
                     /gene="ruvC"
                     /locus_tag="PLQ68_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HXK71975.1"
                     /translation="MRILGIDPGFAIMGYGVIDYYKNKVEVLEYGVIRTEAHTDFNKR
                     LLKNYQGINEIISLYNPEHISMEQLFYNTNQKTVINVCQARGVAVLAVAQKDLPFFEY
                     TPLQIKQALCGYGRADKNQVQQMVKVLANLKNIPKPDDAADALAVAICHAHSYREERN
                     NKNTCTSI"
     gene            5849..7078
                     /locus_tag="PLQ68_04650"
     CDS             5849..7078
                     /locus_tag="PLQ68_04650"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXK71976.1"
                     /translation="MDGKLWTRDFSILTIGSAVSMLGNAVSGFALGLLILDYTNSTFL
                     YSLTLVIYTLPRIVMPLLAGPYLDRFSRKKIIYTLDFISAALYLLIFALLFFNFFNYY
                     LIIILALIIGSIDGTYIVAFDSFFPNVVEKKNYSKAYSISSILYPLSAVMVPVAAYFY
                     ENFSLEPLFLFNAVTFLIAALFEVNIKAEEKHINTDIVKSFNFLKYKEDFKAGIKYLK
                     QEPGLAAITAYFVIMAFSDSSYGTLVLPFFKNNETFGVMTYTYVMAFAVLGRLIGGFI
                     QYRFKYPPKRRFSIAIFVYASIGIMSAFFLFSPIVVMIIMNFLIGIFGVTSYNIRISS
                     TQNYLPDGMRARFNGIFQMLTMMGAIVGQLLSGGLGDIFDERYIVAGFNIINLIMLIV
                     IMVPMGKHIKKIYNTEI"
     gene            complement(7127..7372)
                     /gene="secG"
                     /locus_tag="PLQ68_04655"
     CDS             complement(7127..7372)
                     /gene="secG"
                     /locus_tag="PLQ68_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HXK71977.1"
                     /translation="MSETLKIVLGIIHLIICIGVIVIVLMQSGKAGGLSGSIAGGAET
                     FFGKGKGKAMDKMLSKYTVYIAAFFAVTSIFLYLVLK"
     gene            complement(7501..8346)
                     /locus_tag="PLQ68_04660"
     CDS             complement(7501..8346)
                     /locus_tag="PLQ68_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012201129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HXK71978.1"
                     /translation="MAKFHTFDTAWCPGCGNFSILECLKTALEELKKDPSEVLLVAGI
                     GQAAKIPQYINANAFCGLHGRALPAATGAKIANEKLTVIVSTGDGDSYGEGGNHFLHN
                     IRRNIDIAHFVHDNQVYGLTKGQASPTSDEGLVTSIQPEGNVNTPLNPLLLAIAAGAG
                     FVARGFTGKKEHLVSLMKQAIGYKGYALVDIMQPCVSFNKTNTYAWYNERVYMLDENY
                     DSSDKLLAIKKAMEFTDKIPIGVIFKEEKPSFNEKNSVLKDSKPLIDRKYDQTITSRL
                     FKEFI"
     gene            complement(8348..10006)
                     /locus_tag="PLQ68_04665"
     CDS             complement(8348..10006)
                     /locus_tag="PLQ68_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007062896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HXK71979.1"
                     /translation="MYNLLIGGAAGQGIDTTSAILEKILKRAGYSVFTCRDFMSRIRG
                     GHNFYLMRFGEKEVISHSYNLDGIIAMDEETINIHSNELKKDGFILCDTSIEIEDSRI
                     LKIAMEDIAKKLGNIRVSGSIAIGAVLKLFNEDYSNIEEVLQSFLDERYVDINLKAVK
                     AGYDSIETRYPRLKGRFDDWMLLSGSNAIALGAIAAGIKFYSAYPMSPATPIMGYLAT
                     KGRDCGIVVEQAEDEIAAINMAIGASYAGARSMTATSGGGFSLMVEALGLSGMAEIPL
                     VVVNMQRPGPATGLPTRTEQGDLKFMISASQGEFPRMVIALRTHKDAFYQTVRAFNLA
                     EKYQIPVILLGDQYLGDASATVEPYEINSIETEYSNSEYSNEYLRYKYTKSGVSPRLI
                     PMKSKNLVTIDSDEHDETGKITESAKVRVQMMDKRMKKLILIEQELHEPQFLGAVDYD
                     ILLIGWGSTYGPISEAIELLNSKKSKKYAALIFGDIFPLPQKLLIEKSKKAKQIINIE
                     QNATGQLAGLIREYTGIICSKSILKYDGRQITGEEIVKRIKGGI"
     gene            10268..11695
                     /locus_tag="PLQ68_04670"
     CDS             10268..11695
                     /locus_tag="PLQ68_04670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK71980.1"
                     /translation="MDTVNFGDNRWKISYGKYEKMEKKAIDIVSAAVSKHVPYVVTTQ
                     KVLSDLEDNIIFIGTRETNNYIKDLILEDSIPQNGYLLKVTDNPYNKNKQIVIIAGID
                     DISTFYGAVDFSSDYIAFSKQRKDHQPYFRKLFLEKMPDYEISSSPDIMNRGLWTWGH
                     SIYNYKKYIDNMALLKLNQLIIWNDYAPVNAKQVVDYAHENGIKIIWGYSWGWGEKIN
                     ISQNEELDRWADLAVKKYEEEYYALGGDGIYFQSFTETLSETKDGKLIGEAVSEWVNV
                     IGSRILDKYPDLYIQFGLHATSVKNQLEYISKIDKRISIIWEDCGAFPYQYLPKHIEN
                     FEDTVDFTDKIIQLREDAEVGVVLKGQICLDWSNFEHQKGPFILGMNDKTVITKLMNE
                     KKEIWHYIQSYWFKNGQLCQDIIRKLSIQTNKEAVIAALVEDGLFEENIWLPVALYAQ
                     LLWDINTPYYDTLCLVSQRDNVILA"
     gene            complement(11749..12855)
                     /locus_tag="PLQ68_04675"
     CDS             complement(11749..12855)
                     /locus_tag="PLQ68_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004629062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HXK71981.1"
                     /translation="MNKKINVGVVGCGNISGIYFKNLTTVFNEIVNVYACADLIKEKQ
                     EHAVEEYKIQRTMTFEQMLECKDIELILNITQPFNHYDICKRSLLAGKHVYVEKPLSL
                     TFEQGLELIDIAKQKNLLLGGAPDTFMGAGIQTCRKLIDDGFIGDIVGATAFMMSKGH
                     ESWHPDPEFYYKPGGGPMFDMGPYYITALINLMGGVKTVVGMNHTAFKERTITSQPKY
                     MQKIQVETPTHVSAMLLFQSGATASIITTFDCKKSELPLIEIYGTKGSIKVPDPNCFS
                     GPVFLATSDGSGYKEVPLTHIYSENSRGLGLADLASCINNRKSPKAGCQQTGHVLEIM
                     CAVFSSFESRKYYKMKTRYKRPEPMNRELVKGQV"
     gene            complement(12883..13542)
                     /locus_tag="PLQ68_04680"
     CDS             complement(12883..13542)
                     /locus_tag="PLQ68_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018750250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HXK71982.1"
                     /translation="MKKALIMWGGWLGHEPEQVGKIFQDVLKEEGFEVTRTDKMDVLA
                     DLENLKQFDLFVPIWTMDEIKKEYVDNVSEALGKYGTGLAGNHGGMCDSFRQSTKWQF
                     ITGSQWVDHPGNDGIEYMVNIKKSSDSKIIEGINDFKVVSEQYYVHVDPAVNVLATTR
                     FPVYEGYYATNGCVDVPVVYTKKWGKAKVFYNSLGHVANVFDIPEALELMRRGFIWAA
                     R"
     gene            complement(13601..15556)
                     /locus_tag="PLQ68_04685"
     CDS             complement(13601..15556)
                     /locus_tag="PLQ68_04685"
                     /inference="COORDINATES: protein motif:HMM:NF012493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HXK71983.1"
                     /translation="MSKKYLLENTSAEATFLYSLFKTLFERNISPSKKPVLKNRLIKM
                     SSSLTYAANQRITEKNAEYYLKRNRHMVYSSIPYIDRLDISNDCLDSIYKKMATCELY
                     LYDRYKTGSNEYPDMFELFEDKRNISQLRSNTVLSIYMELYLKKMAKEQIIRLIPSNP
                     KDEFPETRKMRRNFVIHLGLTNTGKTYHALEALKEAKSGAYLSPLRLLALEVQERLME
                     DNVLCSMLTGEEEDIIDGAKHISSTVEKADYTQHYDVVVIDECQLIQDDSRGCYWTKA
                     ILGMKAEIMHLCAAPEAEDILVKIIKDCQEEYEIIRHVRNTELIFSKEPFSLKNTQKG
                     DALVVFSRRSVLSIAEQLSQNGRKASVIYGALPYKTRKEQLKMFTEGKTDVVVATDAI
                     GMGLNLPIKRIVFLETIKYDGNSTRLLVPWEVKQIAGRAGRRGLYEKGYVSSVSDNEM
                     IKKHLSIPNVPIEYAYLGYSDVLLDIDCDITQALKVWKSIPTGGFYVKQDISRTINLC
                     EQLKKIYKGFSKRELLKLASIPFDEENKEVMDLFKEYVLLYSYDQELFKPHIFSYTLN
                     ALEDYYKCLDLYYSFSKNMGYVPDLMWLRETKMMIAERINKELINHSSVKKRRCSICN
                     RLLGRNDKTDLCVSCQKKLIDVDLESY"
     gene            complement(15556..16017)
                     /gene="rnhA"
                     /locus_tag="PLQ68_04690"
     CDS             complement(15556..16017)
                     /gene="rnhA"
                     /locus_tag="PLQ68_04690"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013788024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HXK71984.1"
                     /translation="MSKDEKSCTNVEIYTDGACINNPGPGGYAAILIHNGIEKEITGY
                     KEKATNNEMELMAALQGLMQLNKSCEVKLYSDSAYLINAFNQKWIDSWKNNGWKRGRN
                     DEIKNLQLWKLLYEMNIKHSITWIKVKGHSDNEYNNRCDKLANKAIKENKI"
     gene            complement(16027..16803)
                     /gene="proC"
                     /locus_tag="PLQ68_04695"
                     /pseudo
     CDS             complement(16027..16803)
                     /gene="proC"
                     /locus_tag="PLQ68_04695"
                     /inference="COORDINATES: protein motif:HMM:TIGR00112.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
     gene            complement(16796..18049)
                     /locus_tag="PLQ68_04700"
     CDS             complement(16796..18049)
                     /locus_tag="PLQ68_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HXK71985.1"
                     /translation="MKKIELLAPAGNIEKLRFAFAYGADAAYLGLNRFSLRDKADNFS
                     YDDLEKAILYAKELNKKIYVTLNILPKNKDIEDIAKTMSELTQFKPDALIIADIGLIE
                     MAKKYAKGIDIHVSTQANILNVESAKVFCKLGAKRLILARELSLDDVGRIKDAVGKEH
                     EIEIFIHGAMCVSYSGRCLLSSYMAGRDANQGDCAQPCRWKYALMEEKRPGQYFEVYE
                     DNSGSYILNSKDLNLIKHIPQLLESKADSFKIEGRMKSSFYVATVCKAYRSAFDDYLE
                     NPEKYYSNIEYYNQIVSMPSHRQFTTAYNINDEKDYIQKTKDSMIYSDSSYIRSHTYT
                     GLVQNYDEKTFLAKVYEINPVFINDEIILMSPHFKNYRQTVSMLYDKDMNPIDKANHP
                     GMVYYIKTDIPVEKDTIIIKEVSNE"
     gene            complement(18046..18723)
                     /locus_tag="PLQ68_04705"
     CDS             complement(18046..18723)
                     /locus_tag="PLQ68_04705"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HXK71986.1"
                     /translation="MYRYTQNNMEKRFNIVNIDVEEYIQSTLKPLEGFLKEMSEHAKK
                     NNIPVAKLETIRALEVIFNLKKPKAVLELGCAIGFTSIFFAGYLDKSGYIDTVENDPD
                     MVKIAKSNIKNSGFEKTINIIYAEAYDYLMNIDKKYDIIFIDAAKGQYEKYFDICSNL
                     LNEKGVIIADNVLYRGMVAKKDKIPHRQATLVERLRSFLDKITKDERFDTSILSIADG
                     LSISVKK"
     gene            complement(18701..20188)
                     /gene="mltG"
                     /locus_tag="PLQ68_04710"
     CDS             complement(18701..20188)
                     /gene="mltG"
                     /locus_tag="PLQ68_04710"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HXK71987.1"
                     /translation="MTKIFNKKQDEAESADEDIIKHTDQIEVEEIEDNDALYESDVVQ
                     QDEHADNQETDEEIADDSDVDNYIEDKDFQEKKTNKFLMFLDGIWNIFSKEEIAKRKE
                     KRRNKVVKIVVYKPKSQFRKFITLMIWLVFLFALGFVFYKGYTFTNTLVKQANSFNAA
                     EAIGVIAEENEKVIDIPKGSTTKDIAKILMDEGVITNDTYFWVYSLLMGNEGNYKSGK
                     HIFDVSVTYDSGEIDLITAQGYEMLIHILSQDSIANPTKKIFFKEGLTITQTVNLFTE
                     NGVGDAQDFYDTCNNFEFEYDFIDQIPKDSRRTNRLEGYLFPDTYIFDVTSDESEIVK
                     KMLDNFDSKFNKEYRQKAEEMGFTIDEILIIASLLEKEASSMDERAMIAGVIYNRLKS
                     EDPTMNYLQIDATIQYLYINETGAVKEFLTYEDHDIMNNYNTYMYKGLPPGPICNPSL
                     SAIAAALYPETHYYYYYVAKNDGTDAHAYATTLAQHNYNVSLYSK"
     gene            complement(20256..21287)
                     /locus_tag="PLQ68_04715"
     CDS             complement(20256..21287)
                     /locus_tag="PLQ68_04715"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HXK71988.1"
                     /translation="MISKTIVKKVLEFDNPERIGYDFLDGSIRDIKYVELNIPKKEEH
                     KWQKPEFFADKYPKYKNFEGYLHLDEYNNLWGKMWHDTTGGGEVLEGGLESWDDLSSY
                     KLPNIDDETRFKDAHLSVEPYKERFILGPLPGCTFAIMRNVRKMEVFLEDLILEEENV
                     LKLQDMVISKLVNMVDLYGSMKCVDGIFFCEDWGTQDRLLVSPAMWNKIFKPAYVKLC
                     KAAHRNNLKVLMHSCGFIYEILDDLLDAGVDAFQLDQPTLMGIERLAEKINLKATLYS
                     PVDIQAVLPTGNKQLIEKSADQMVDLFHKKGGLMVKDYGDYNTLRIKDEWAGWARNRF
                     FEIGGKDPR"
     gene            complement(21321..25085)
                     /locus_tag="PLQ68_04720"
     CDS             complement(21321..25085)
                     /locus_tag="PLQ68_04720"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010964962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HXK71989.1"
                     /translation="MLNNVRRIFVERKDGFDLEAKRLHEQCVTLLGIEGIRGIRILNR
                     YDIQGLDDSIYAVAKYGIFAEIQTDNVYEEAVDFNRYEHIFAYEYLPGQYDQRADSCE
                     QCIQLISHHRPIVKTAKLVCIFGDIKNNDIDKIKEYIINPVECRLASMEKPDNLKSHY
                     NVPYMVENVSGFIKSDKDAIVKMKNQCGFAMSEDDLLFVQEYFKSLDRDPTITELKVI
                     DTYWSDHCRHTTFNTVLDEVVIDEDIFNDKIKKAYERYIEIRKELYKDKDKPICLMDI
                     ATIGAKYLKSKGLLDDMEESKENNACSIVADVPTSKGDEKWLIMFKNETHNHPTEIEP
                     FGGAATCLGGAIRDPLSGRSYVYQAMRVTGAADPFSKIEDTLPGKLPQIKIIREAANG
                     YSSYGNQVGLATGQVDEIYHNGYIAKRMEIGAVIAAAAIENVKREDPCTDDVIILIGG
                     RTGRDGLGGATGSSKVHTEESLSECGAEVQKGNAPTERKIQRLFRNPDMSKLIKKCND
                     FGAGGVAVAIGELADGLEIYLDNVPKKYEGLDGTELAISESQERMAVVIDKSDMDKVF
                     ELAAFENLEATYVAKVTDTEKLKMIWNGKVIVNIERSFLDTNGVTQHAKAHVTKKDSS
                     SNPFINKSDKSYEKQLFESVNDINSCSKKGLIEKFDSTIGSNTVLMPLGGKYQITPSE
                     AMVAKLPVLDKKTDYCTVMSYGFFPNISEWSCFTGACYSVLSSVSKIVSVGGEWRKTR
                     LTLQEYFESLRKDAKRWGKPFQALLGALYAQTELGIPAIGGKDSMSGSFMDMDVPPTL
                     VSFAVSHTSAGNIISSEFKKTDNYILRLRHRRNADMSPDFQYAKKIFDLLQEFNSNSL
                     ILSSSTIKHKSAAEALLKMCFGNMIGFVLTANEESLFDNGYCDILIEIDSQKISNIND
                     FLSRYDNLDLEVIGKTIDESKFIMKDIVVDLETLLTEYTSKYESVFSTKSIAEKPNIT
                     NKPYEYKKVFHSKINIAKPRVFIPVFPGTNCEYDTKRAFEDQGAICSTVIFRNTRAND
                     IDVSVKEFVKLINDSNIIAIPGGFSAGDEPDGSGKFIATVFRNPYIKEAVHNMLYSRD
                     GLMLGICNGFQALVKLGLIPYGKISELSEKSPTLTYNSIGRHVSCMVNTKIISKLSPW
                     FSCVNLNDIHTIPVSHGEGRFICTEQEYNLLSANGQIATQYVDDSGNPTYDIAYNPNG
                     SYYAIEGVTSKDGRILGKMGHSERFGNNVCINVPGNKDQKLFESGVKYFK"
     gene            complement(25135..26964)
                     /gene="htpG"
                     /locus_tag="PLQ68_04725"
     CDS             complement(25135..26964)
                     /gene="htpG"
                     /locus_tag="PLQ68_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003446839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HXK71990.1"
                     /translation="MNEKGNISINTENILPIIKKWLYSDKDIFLREVVSNAIDAITKH
                     KKVVAIGEVEKPDKYRIDVKVDKKNKTITISDNGIGMTFDEVKQYINQIAFSGAIDFF
                     EKYKDKTDAQDQIIGHFGLGFYSVFMVSELVEIQTKSYLKDESAVYWKSDGASGYEMN
                     EGTRQFHGTDIILHINSDKENKDLINVWKMREILEKYCKFLPIEIYLSEEGAKKDDKQ
                     EKEEEKPINNTSPLWLKDAKDCTDEEYKEFYREVFHDFKEPLFWIHLKMDYPVNLKGI
                     LYFPKLTNEYNTLEGQIQLYCNQVYVADNIKEVIPEFLLLLKGVIDCPDLPLNVSRSF
                     LQNDGYSTKIAGHISKKVSDKLKQLYKNDTEQYYKYWDDINVFVKYGCLKEDKFYDKV
                     KDSVVYKDIYDKYYTLDEYKKVSEDKKQKDKIYYVSDKQKQAQYINMFKENDLNAVIL
                     DNILDSHFISFIESKNTGTSFIGIDSGIKEVLGEDTKEEGFDDVKKYFEDMLNNKNVT
                     VELKSLKTSDVPAIISANEYENRMKSMQTMYGMDIPSTDILVLNTQNTVVKKLMEMTD
                     EDKKKDVAMYIYQLAMLSAGKLEKEDMTDFLRRNNEMLNKSVD"
BASE COUNT         8412 a         5082 c         3747 g         9817 t
ORIGIN      
        1 ataaagagga tacgcgcaaa actgccactt gaaatacaaa aatgcataca aactatttat
       61 ggtgtaggtt ataaattcga atgtgtaaat gaataaactt attggtaaac cttttttctt
      121 tagaatcact gctttagtat taacaatact aattatttct ttaatattca ctacaataat
      181 atatacagtt gcttctgaag agatttttat cagttaccag accgaaaggc ttaaagaaga
      241 aacagatatg ctcgcttctt taataccaaa tgaatggaca acaaaggatt tgttagaaca
      301 gttttttgat aaagtctttc aagtcgaatc aacaatttta agcagttatg ttgtaatatc
      361 agataagatt aatgtagaga tgtattcata tgtcccttct tcgcaaagag ctggcattgt
      421 aatagataaa gatgcaatgg acagatttta taagcagaat ctatctcaat atttcaaaag
      481 agtgcttagt ggccagtata tatctcttga aaataaaaac aatgaattcg acaaagaaat
      541 gattattctt atgagaccta tattgtctga tacgggctct gttgaagcag ttcttactgt
      601 atgtaaattt gtggaagatt atagtattca actggaaaat ataaacaaaa ctctcattat
      661 tgcaatgttt gctgtttttt tactcatgct atatccagtt tatatgctat caaaaagcat
      721 aacaaaacca ttatttgaaa ttaaggatgt cgcaattgca atgggtaagg gtgatttttc
      781 agttagagca aatgaaaatt atcctgctga aataggacag ttatcgacta ctatgaatct
      841 cttatcctca gaattagatt ctacaattaa tcatttaaaa agagagacaa atgttctaca
      901 aggcgtttta aattctatga atgaaggaat tttagtaata gataagcatt ttaatccaat
      961 aataataaat ccagcaacag gtaagctttt taacacggat attaaatcta gcgataaatt
     1021 aaatataatt ccgaaagaag aaatatggaa agattttgaa aaatgcattg agactaatat
     1081 gtctgtagac aatacatata actttgatga aattatgatt cagtgcatca ttaaccctgt
     1141 tatagtaaac aatgaaattg ttggtgctat gggtatcttc agtgatataa caaaagaagt
     1201 tcgtctcgaa cagacaagac gagaatatgt tgcaaatgtt tcacatgaac ttaaaacacc
     1261 acttacagga gtaatgggac ttatcgaacc tttaaaagac ggattagtaa cagacaatga
     1321 taaaattaac agatattacg aacttatatc caatgaggtt atgcgactca acaggcttat
     1381 taatgattta ttagttttgt cgcgacttca atcatcgaat gaagcatttt cgatggaaaa
     1441 agtaaacttg actgaaataa taaatgatat tgtagagaga tacctctttt ataacaaaga
     1501 tcaaattttt aatataaaat gtaacatccc taaagaacct gtatttattc ttgggaatgg
     1561 tgatcgtata gatcaaatac taactatctt agttaataat gcattaaagt ttggtaaaga
     1621 tgatgttgat atagctcttt gtatagaaat atctttaact aaagaaaata acaaatacat
     1681 cgtatcagtt gctgataacg gaaaaggtat ctccaaagat gatcttccat atatttttga
     1741 aagatttttc aaaactgata aatcccgttc aaaaggcggt tccggactag gactttcaat
     1801 agcaaaagaa actcttcttc gcatgaacga aaaaatatgg gtcgaaagtg ttgaatcagt
     1861 tggatcagtt ttcaaattca cattaagcga ctatttaata taattattat atttttactg
     1921 aatatttatg aacgtaatta taaactcctg caataccgca atatgatctt attgctgcat
     1981 tcatgctcca ttcattttca aattcgaata ttaatggttt tcgaagaata tcttgtatct
     2041 gaataattaa ttcagaaata aaatcagcat tatcagattt atatgataaa acgaatgaat
     2101 tcacattttt ttcagatata tcatatgctt tgtcaataca ttcaagcata ttttcattat
     2161 caataatcca ctcgtaagta ctaaatggtt tttctatgtc aatataagaa tattgtgaca
     2221 ttatatactt atgactatca atttcaacag taacgctccg agttctctct ttaacataat
     2281 tcattgattt aatatactca ggtgtagtac cacaacaacc accggttatt ccgatgttat
     2341 attgcaataa atcgtcacta taactacaga actgttcttt tgtctgacta taatacgctt
     2401 tattatctac aaaacttggt actccggcat ttggtttgac acagacaaag tcttcaaaac
     2461 atgctatctg tttaagtagt tcagacattt tttccggtcc gttagaacag tttactccaa
     2521 ctacatctgc tcccattttt ttcagtattg cagagcatat aaaaggggta tgtcccatca
     2581 tagttacatt attcttctca tatgtcatat tacttataat aggaagattg actgtatcct
     2641 gattagcgat aattgctatc ctcatttctc ttaaatcagt aaatgtctca aaattaagaa
     2701 catcagcttc tttgcaagag tcaatctgtt ctacaaacaa atcatatgcc tgattgaatt
     2761 taaggttgcc taagggttcg agaagctctc caataggtcc tattgaagca gcaactaata
     2821 tttttctact acctatcgcc tgctttgcta aattaactga tgtattatta atttcataaa
     2881 ttttatcaga taagttatga cgttctagaa cggatctatt tgaacacaat gtattagtct
     2941 gtattatgtc actacctgca tcacaatact cattatatat cctcatcaca atatctggat
     3001 ttgatatatt gtattcctct gctaattcac cagtctttaa tccatatttt tgaagcatat
     3061 agcctttgga accatcaaat aaatatatct tattcttatt gaataatctc ataaatgtta
     3121 cctactcttc ccatgttatc ttatctttaa acttagatga attatatgaa tttatacatc
     3181 cacaatactt ctgcctataa agaccaattt gtcttgcttt attctgtcct tgtctgaatc
     3241 cttttctaaa atcatcatat aaaaactcta tatcatattt aatcgctaga ttattacaaa
     3301 tctcaattat tttttcatgg tcctgatata tactaactaa aagtgtagta ctaaatccag
     3361 agtatccgtt ttcttttgca tatttagctg tcttttccag ccttatatta taacaatcta
     3421 agcatttaaa tccttgctcc catttttcct gtaaaaactc atcatcaaca ataagcttaa
     3481 cattaaactg ttcagtataa ctaataagag tttttaatct tcttttatat tccaaaatcg
     3541 gatgaatatt cggattgtaa aaatatgaat caaactcaac agataattca ttcaatctat
     3601 catgcacata cacaagacaa ggagcacagc atgtatgaag tagtatttta ttcgtgttca
     3661 tatggtaatt tcagatgctc atatgctaat cgggtaacgc atcttccttt cggtgttttc
     3721 ttaagaaaac ctaattgaag aagataaggt tcatatacat cttcaatagt ttctttttcc
     3781 tcatttatac tggctgaaag agtatccagg ccaacaggtc ctccagtaaa ttttgttatt
     3841 attgtatcta aaatcgctct atcaatatta tctagtccaa ttttatcaat ttcaagtttt
     3901 cccaaaccaa aatctgctat ttcacttgta attacatctt ctctctgtac ttgtgcgaaa
     3961 tcccttattc ttctcaaaag cctgttagct atcctaggag ttcctcttga tcttcttgct
     4021 atctcatttg ctccgtactc attaatattt ataccaagaa ttcctgcgct tctttttaca
     4081 atcatactta attcatctgt actatacatt tccagcctgc atgaaacacc aaaacggtct
     4141 ctaagaggag aggaaagcat cccagtcttt gttgtagctc ctataagagt aaatctattt
     4201 aaatccaatc ttatcgtttt tgcacttggc ccttttccaa taataatatc tagagcaaaa
     4261 tcttccattg cagggtaaag aatttcctca actgtacggt taagcctgtg aatctcatca
     4321 atgaacaaga tatcattttc atcaatatta gtaagtattg ctgcaagatc acctgctttt
     4381 tcaatagctg ggccactggt tattctaata tttgcaccca tttctgatgc gattattcca
     4441 gctaaggtag tcttacctag acctggaggt ccagcaagaa gtacatggtc aataggttct
     4501 tttctctgct ttgcagcttc tataaaaatt ttaagattct gctttatctt ttcctgtcca
     4561 aaatactctt caaaaacctt tggtcttaat cctgattcac tctgatcgtt ttttataaat
     4621 gtcggagtta caaatctttc ttcctccatt atcacatctc cttgttactg cttgttcttc
     4681 cagcaagagc attcttaatt atatcctgca atgaatcttt ttcgtcatag actgcatcaa
     4741 gagcctgtct cgcatactta ctattatacc ctaaaactaa taatgcattt actgcttctg
     4801 agtatttatc actttgttct atctgccctt tgggttgtgt gtcattaata taaatacctt
     4861 ttaacttgtc cttaagctct agtataatcc tcatcgcagt cttttttcca attcctggag
     4921 ctttagacaa taaacctaca tcatcactaa caacagcagt tgctacatct tggtatgaaa
     4981 gaacagataa tatggaaaga gcagcttttg gaccaattcc tgatatagta attagcatat
     5041 taaataggaa aagctcttct tcctgtgaaa atccataaag aatcatcgca tcttccctaa
     5101 catgaagata cgtatatagc ataacatcct cgtcagtctt tactttcatc atagtattta
     5161 agcttgtata tatcttatag ccaataccat tattctcaat aacgacataa tcatgttttt
     5221 ttgctgtgag atttcctttt atatactcgt acatgtattt ttattattcc tttcttccct
     5281 gtatgaatgt gcatgacata ttgcaacagc cagtgcatct gcagcgtcat caggttttgg
     5341 aatatttttt agattagcca ataccttaac catctgctga acttgattct tatcagctct
     5401 tccataccca catagagctt gttttatctg aagcggagta tattcaaaaa aaggaagatc
     5461 cttctgtgca actgcaagaa cagctacccc tctcgcctga catacattta tgactgtttt
     5521 ctgattagta ttataaaaca actgctccat agagatatgc tcaggattat aaagagaaat
     5581 tatctcgttt attccctgat aattttttaa tagtctttta ttaaaatcag tgtgtgcttc
     5641 tgtccttatt acaccatatt cgagaacttc gactttgttt ttataataat caattactcc
     5701 atatcccata atggcaaaac caggatcaat ccctaatata cgcatgaata ctcctcgttt
     5761 tctaattata acatgtagcc tatgttttgg cacgaaacaa ttgaacttga aaaaataaag
     5821 tgctaatata tacgaattga ggtttattat ggatgggaaa ttatggacta gagacttctc
     5881 tattctgaca ataggcagtg ccgtatccat gcttggtaat gccgtatctg gatttgcttt
     5941 aggattattg atacttgatt acactaattc tactttttta tattcattaa ctttagtaat
     6001 ttacactctt ccaagaattg ttatgcctct actagctggc ccttatctgg atagattttc
     6061 aagaaagaaa ataatatata cattggattt catttctgca gctttatact tattgatttt
     6121 cgctttacta ttttttaatt tttttaacta ctacttaata attatacttg ccttaataat
     6181 tggttctatt gatggaacat acattgttgc ttttgatagc ttctttccaa atgtagtaga
     6241 aaaaaagaat tactcaaaag cttattctat atcttcaatt ctttatcctt tatctgcagt
     6301 aatggttccg gtcgctgcat acttctatga gaacttcagc cttgaaccgc ttttcttgtt
     6361 taatgcagtt acattcctta ttgcagcttt gtttgaggtt aatataaaag ctgaagaaaa
     6421 acacataaat acagacatag ttaaatcatt taatttttta aaatacaagg aagattttaa
     6481 agctggtata aaatacttaa aacaggaacc aggtttggca gcaataactg catattttgt
     6541 tattatggct ttttctgact cttcttatgg tactttagta cttcctttct ttaaaaacaa
     6601 tgaaactttc ggagtaatga catacactta tgttatggct tttgctgttc taggtcgctt
     6661 gataggaggc tttatacaat accgattcaa gtaccctccg aaaaggcgat tctcaatagc
     6721 aatattcgta tatgcatcta ttgggattat gtcggcattt ttcctgtttt cacctattgt
     6781 tgttatgatt ataatgaact ttcttattgg aatatttggt gtcacttcat ataatataag
     6841 aatatcttca actcagaatt acctgcctga tggtatgagg gctcgcttta atggcatatt
     6901 ccagatgctt acaatgatgg gtgcaatagt aggtcaattg ttatcaggag gtttagggga
     6961 tattttcgat gaaagatata ttgttgcagg atttaatatc attaatctga taatgcttat
     7021 tgtaataatg gttccgatgg gaaaacatat caagaaaata tataatactg aaatataaaa
     7081 aaaggcttat tcagcctttt aatttacgat ataaaattaa aatattttac ttaagtacca
     7141 gatacaaaaa gattgatgtt actgcaaaga aagctgcaat gtaaacagta tactttgaaa
     7201 gcatcttatc catggctttt cctttgcctt taccgaagaa agtctcagct ccgcctgcta
     7261 tacttcctga caaaccgcct gctttacctg actgcatcaa tactataact ataactccga
     7321 tacatattat taagtgtatt attcctaaaa ctatctttaa tgtctcactc atttgctacc
     7381 tccaaaactc aactctacaa atataacata aattattttt tatagcaagt acattttttg
     7441 attattcgag tcaatcgata tgtcattatt tcttgaaaat atataaactt attttcattt
     7501 tcatataaac tctttaaaaa gcctgcttgt aatagtttgg tcatacttcc tgtctatcaa
     7561 tggcttgcta tctttcaaaa cagaattttt ttcattaaat gagggttttt cttctttgaa
     7621 tataacacca attggtattt tatctgtaaa ttccattgct tttttgatag ccaataactt
     7681 atctgaagaa tcgtaatttt catccagcat ataaactctc tcgttatacc aagcataagt
     7741 attcgtcttg ttaaaactaa cacatggctg cattatatca acgagcgcat accctttata
     7801 ccctattgcc tgcttcatca gtgatactaa atgttctttt tttccagtaa atcctcttgc
     7861 aacaaaacct gctcctgcag caattgctaa aagtagcgga ttaagcggtg tattaacgtt
     7921 tccttcaggt tgaattgatg ttacaagacc ctcatcggaa gtgggagatg cctgcccttt
     7981 cgtcaggcca tacacctgat tgtcatgcac aaaatgtgct atatcaatat tacgtcgtat
     8041 gttgtgtaaa aaatggttcc cgccttctcc atatgaatca ccatcacctg tactaactat
     8101 aacagtcagc ttttcatttg cgatctttgc tcctgtggca gcaggtaatg cacgtccgtg
     8161 caacccacag aatgcgttcg catttatata ctgtggaatt tttgcagcct gtcctatacc
     8221 tgcaactaga agcacctcac tgggatcttt tttaagttcc tccagtgcag ttttaagaca
     8281 ttcgagtatg ctgaaattcc cgcatccagg acaccaagct gtatcaaatg tgtgaaattt
     8341 agccattcta tatgcctcct tttattctct ttacaatttc ttcacctgtt atttgtcgac
     8401 catcatactt taatatactt tttgagcaga ttatacctgt atactcacga atcaaaccag
     8461 ctaactgtcc agttgcattc tgttcgatat ttataatctg cttagctttt tttgattttt
     8521 ctattagtag tttttgtggc agcggaaata tatcaccaaa tataagtgct gcatattttt
     8581 tacttttttt actatttagt aattcaatag cctctgatat aggtccatat gttgaccccc
     8641 aaccaatcaa cagtatatca tagtcaactg cacctaaaaa ttgtggttca tgaagctctt
     8701 gttctattaa tataagtttt ttcattcgtt tatccatcat ctggacacgt acttttgcag
     8761 actcagtgat tttacccgtt tcgtcatgct catcactgtc tattgtaaca agattctttg
     8821 atttcattgg aatcagtctg ggtgaaactc cactcttagt atatttatat cgtaagtatt
     8881 cattagaata ttcgctatta gaatactctg tttcaatact gttaatttca tatggctcaa
     8941 cagttgcgga agcatctcca agatactgat ctccaagaag aataaccggt atttggtatt
     9001 tttctgctaa attgaaagca cgtaccgtct gataaaaagc atccttgtga gttcgtaatg
     9061 caatgaccat tcttggaaat tcaccttgtg aggcagatat cataaactta agatctccct
     9121 gttctgtacg tgtcggtaat ccagttgcag gtcctggcct ttgcatgtta acaacaacta
     9181 atggtatctc agccatacca gaaagtccaa gtgcttccac cataagggaa aaaccacctc
     9241 cagaagttgc agtcatagat cgcgctcctg catatgaagc accaattgcc atgttaattg
     9301 ctgcaatctc atcctcggcc tgttcaacaa cgataccaca atctcttcct tttgttgcaa
     9361 gataacccat tattggtgta gctggagaca tcggatatgc actatagaat tttattccag
     9421 ccgcaattgc tcctaatgcg attgcattac ttcccgagag caacatccag tcatcaaatc
     9481 gacctttcaa gcgaggatat cgtgtttcaa tactgtcata acctgccttt actgccttaa
     9541 gattgatatc tacataacgt tcgtcaagaa aactttgaag cacttcttca atattcgaat
     9601 aatcttcatt aaaaagcttc aaaacagctc ctatcgctat actgccagaa acccttatat
     9661 ttccaagttt ttttgcaata tcttccatag caatttttaa aatgcgtgaa tcttctattt
     9721 ctatacttgt atcgcacagt atgaaaccat cttttttaag ttcattactg tgtatattga
     9781 tagtttcctc gtccattgca attataccgt caagattgta actgtgagaa ataacttcct
     9841 tttcaccaaa acgcataaga tagaagttat ggcctcctcg tattcgtgac ataaaatccc
     9901 tacaggtaaa cacgctataa cctgctcttt tcaatatttt ctcaagtatt gcagaagttg
     9961 tatcgatacc ttgccctgct gcaccaccta tcaatagatt gtacatatat agtcctcctt
    10021 ctaatattct tcatatctat atatctatca gatccgcaaa atcaactcgt tttaaggttt
    10081 agtgatgttt gttaattcaa tttattattt atttcaatac ttataatatc acaagacatt
    10141 gtacaagagc aaaaaattat tatatagaaa ataaagtgtt ttaattttat aattaaattc
    10201 atactaaatc cttaaaattc tatttaactt acaaatttaa tattgtatac ttttgtctgg
    10261 aggaaaaatg gatacagtaa attttggaga taacagatgg aaaatttcat atggaaaata
    10321 tgaaaaaatg gaaaagaaag ctatagatat tgtaagtgca gcagtttcaa agcatgtacc
    10381 ttatgttgtt acaacacaga aggtgttatc agaccttgaa gataatataa tatttattgg
    10441 tacgagagaa acgaataatt acataaaaga cttaatctta gaagactcaa tccctcaaaa
    10501 tggttattta cttaaagtca cagataatcc gtataacaaa aacaaacaga ttgttattat
    10561 tgctggtata gatgatataa gcacctttta tggtgctgtg gatttttcaa gcgattatat
    10621 tgcattttca aagcaaagaa aagatcatca accatatttt agaaaattgt tccttgaaaa
    10681 aatgccagat tatgaaattt catcttcacc tgatatcatg aatagaggtt tatggacttg
    10741 gggtcattct atttataatt acaagaaata tattgataat atggctcttt taaaactaaa
    10801 tcagcttata atttggaatg actatgctcc tgttaatgct aaacaagtag tagactatgc
    10861 tcacgaaaac ggaataaaga taatatgggg atattcctgg ggatggggag agaaaattaa
    10921 tatttcccaa aatgaagaat tggacagatg ggctgattta gcagttaaaa aatatgaaga
    10981 agaatattat gctttaggtg gagatggcat atattttcaa tcctttaccg aaactctaag
    11041 cgaaacaaaa gatggtaaat taattggtga agcagtttca gaatgggtaa atgttatagg
    11101 tagcagaata cttgataagt atccggattt atatattcaa ttcggtcttc atgctacatc
    11161 agtgaaaaat caacttgaat acatttcaaa aattgataaa agaatatcaa ttatttggga
    11221 agactgcgga gcttttcctt atcagtatct tccaaaacat attgaaaatt ttgaagatac
    11281 tgttgatttt actgataaaa tcattcaact tagagaagat gctgaggttg gtgtcgtttt
    11341 gaaaggacag atatgtctgg attggtctaa tttcgaacat cagaagggac cattcatttt
    11401 aggcatgaat gacaaaacag ttataacaaa attaatgaat gaaaaaaaag aaatctggca
    11461 ctatatacag agctactggt ttaaaaatgg acagctgtgt caggatataa taagaaaatt
    11521 atcaattcag acaaataaag aagcagtcat tgcagcatta gtagaagacg gtctgtttga
    11581 ggaaaatata tggcttccag ttgctttata tgctcaatta ctttgggata taaacacacc
    11641 ttattatgac actttatgcc ttgtttctca aagagataat gtaatattag cataacaggc
    11701 ttataagcct gttatgtata taaaaagcat ttataataaa atatactact acacctgacc
    11761 cttaactaat tccctgttca ttggttcagg tcttttgtaa cgtgtcttca ttttgtagta
    11821 ttttcttgat tcaaaactgg aaaataccgc acacatgatt tcaagaacat gaccagtctg
    11881 ctgacatcct gccttaggag actttctatt gttaatacag ctagctaaat ctgcaagacc
    11941 caaccctctg ctgttttcag aatagatatg ggttagtggt acttctttat atcccgaacc
    12001 atctgatgtt gctaaaaata cagggccgga aaaacagtta ggatctggaa ctttaatact
    12061 gccttttgta ccatatattt caataagcgg tagttcgctt ttcttgcaat caaatgttgt
    12121 tataatgctt gcagtcgctc cgctttgaaa tagcagcatt gctgaaacat gggtaggtgt
    12181 ctccacctgt attttttgca tatatttagg ctggctagtt atggttcttt ctttaaatgc
    12241 agtatggttc attccaacta ctgttttcac tcctcccata aggtttataa gagcagttat
    12301 gtaataaggc cccatatcaa acattggacc tccacctggt ttataataaa attcagggtc
    12361 aggatgccat gattcatgac ctttgctcat cataaatgca gtagcaccta ctatatcacc
    12421 gataaatcca tcatcaatca gttttctgca tgtctgtata cctgcaccca taaatgtatc
    12481 tggagctccg cctaaaagaa gattcttctg ctttgcgata tctatcaatt ccaatccttg
    12541 ttcaaaagtc aggctaagag gtttctcaac ataaacatgc tttcctgcta ataacgaacg
    12601 tttacatata tcataatgat taaaaggctg ggttatgttg agtattaact caatatcctt
    12661 acattcaagc atttgctcaa aagtcattgt cctttgaatc ttatattcct caactgcatg
    12721 ttcttgcttc tcttttataa gatctgcaca agcatataca tttactattt cattaaaaac
    12781 agttgtaaga ttcttaaaat aaataccact tatatttcca caaccaacaa ctcctacgtt
    12841 aatcttttta ttcatctttt ttaccgcctt tactatttag cattatcttg ctgcccatat
    12901 aaagccacgt ctcataagtt caagtgcttc aggtatatcg aacacatttg caacatgacc
    12961 caagctatta taaaaaactt ttgcttttcc ccattttttt gtgtaaacta cggggacatc
    13021 aacacatcca ttggttgcat agtaaccttc atatacagga aatcttgttg ttgctagcac
    13081 attaacagca ggatcaacat gtacataata ctgttcagaa actactttaa aatcatttat
    13141 tccttcaatt atttttgaat cagatgattt cttaatatta accatatact cgattccgtc
    13201 atttccggga tgatccaccc attgagaacc agttatgaac tgccatttcg tgctttgtct
    13261 gaatgaatca cacataccgc cgtggtttcc tgctaaacct gtcccgtatt ttcctaaagc
    13321 ttcagacaca ttatcaacat attctttctt aatttcatcc atcgtccaaa tcggaacaaa
    13381 taagtcaaac tgctttagat tttccaaatc agctaagaca tccattttat ctgttcttgt
    13441 aacttcaaaa ccttcttctt ttaaaacatc ctgaaatatt ttacctacct gctctggctc
    13501 atgacctagc catccgcccc acattataag tgcctttttc ataataactg ctcctacttt
    13561 gtttttacgg tacaataata ccgttatcta tatcttatga ttaatatgat tctaaatcaa
    13621 catcaattag ttttttctga cagcttacac ataaatctgt tttatcattt cttccaagca
    13681 ggcggttaca tatgctgcat ctcctctttt tcactgaaga atggtttata agttctttat
    13741 ttattcgttc agcaatcatc atctttgttt ctcttaacca cataagatct ggtacataac
    13801 ccatattctt tgaaaatgaa tagtaaaggt caagacattt gtaataatct tcaagagcat
    13861 tcagagtata ggagaagata tgtggtttga ataattcttg gtcgtatgaa taaagaagga
    13921 catattcttt aaaaagatcc attacttcct tgttctcttc atcaaatggt attgaagcaa
    13981 gttttaataa ttctctttta ctaaatcctt tatatatttt ttttaattgt tcacataagt
    14041 taatagttct tgaaatgtcc tgcttcacat aaaagccacc agtgggtatt gatttccaaa
    14101 cttttaaagc ctgagtaata tcacagtcaa tatccagtaa aacatctgaa tatcctaaat
    14161 aagcatattc tatgggtaca ttaggtattg atagatgctt tttaatcatt tcattatcag
    14221 aaactgatga aacataacct ttttcatata gacctcttct tcctgctcgg cctgcaatct
    14281 gcttaacttc ccatggcacc agtagtcttg tagagttccc gtcatatttt attgtttcca
    14341 aaaaaacaat tcttttaatt ggaaggttca ggcccattcc aatagcatct gttgctacta
    14401 caacatcagt ttttccctct gtgaacattt ttaattgttc ttttcttgtc ttgtatggca
    14461 aggctccata tataacgctt gctttccttc cattctgaga aagctgttca gcaatactta
    14521 atacgctcct tcttgaaaaa acaactaaag cgtctccttt ttgagtattt tttaatgaaa
    14581 aaggttcttt tgagaatata agttcagtat ttcgaacatg tctgataatc tcatattctt
    14641 cttggcaatc tttaataatt ttcacaagaa tatcttcagc ttcaggagca gcacacaaat
    14701 gcattatctc cgctttcata cccaaaattg cctttgtcca gtaacaacct cttgaatcgt
    14761 cttgtattaa ttggcactca tctattacaa caacatcgta atgctgtgta taatcagctt
    14821 tttcaactgt agatgatata tgttttgcac catctattat atcttcttcc tctcctgtaa
    14881 gcatactgca taatacatta tcttccatca atctttcctg cacctcaagg gctagaagcc
    14941 ttaatggaga aagataagcg cctgatttag cttctttcag tgcttctagt gcatgatatg
    15001 tctttccggt attagtcaaa ccaagatgaa tcacaaaatt tcttctcatc tttcttgttt
    15061 ctggaaattc atctttggga tttgatggaa taagtcttat aatctgctct ttagccatct
    15121 ttttaagata aagttccata tagatagata aaacagtatt tgagcgaagc tgagaaatat
    15181 tcctcttatc ttcaaatagt tcaaacatat caggatactc attacttcct gttttatatc
    15241 tatcataaag gtataactca catgttgcca tttttttata aatagaatcc agacaatcat
    15301 tactaatatc taatctgtct atatatggaa ttgatgaata taccatatgt ctgtttctct
    15361 ttaagtaata ttcagcattt ttttctgtaa ttcgttgatt tgctgcatat gttaaagaag
    15421 aagacatttt aatcagtctg ttctttaaaa caggtttctt acttggacta atattccttt
    15481 caaaaagcgt cttgaacaag gaatacagaa aagttgcttc agcagatgta ttttcaagaa
    15541 gatatttctt cgacattaaa ttttattttc cttaattgct ttattagcaa gtttatcgca
    15601 tctgttatta tactcgttat cggaatgacc ttttacctta atccatgtta ttgaatgttt
    15661 aatattcatt tcataaagca atttccataa ctgtaaattt tttatttcat cattccttcc
    15721 tcttttccat ccattattct tccatgaatc aatccatttt tgattaaatg catttataag
    15781 ataagcagaa tctgagtata atttaacttc gcaagactta ttaagctgca ttaagccttg
    15841 taatgccgcc ataagttcca tttcattatt tgttgccttt tctttataac cagttatttc
    15901 tttttctatt ccattatgaa taagtatagc agcataaccg ccgggtccag ggttgttaat
    15961 acaagctcca tcggtataaa tttcaacatt agtacatgat ttttcatctt tactcatctg
    16021 tgcggatctg ttttttgctg ctaatactgc tttttgaaca attagttcaa aattctctga
    16081 atttaaaaca ttaactccct ctattgtagt accattagga gaacagatac ttttaatcaa
    16141 atcttccaga ctatctttct gattaagcgc atattcagct gaacctagaa ccatctgaca
    16201 agccattatc ctggcatcat cccttgatat attatacttt attgcaatat cttcaagcgc
    16261 ttttataaac ataaatacaa atgcaggacc agaccctgca actgctgtgg ttatattcat
    16321 atcagattcg ttttgaacaa caattgtttt tccaacaaaa tcaagaagat ccacagcttc
    16381 gtctaattct tcttttgata cataattacc tttacaaatt gatgacatgc cttttttaat
    16441 tgaagcaggc atattaggca tcacacgaat aaatctacct tcttttattt tattctcata
    16501 aaaattaatt agttttcctg ctgcaatcgt aatgtataaa ggtttatttg atatttcttt
    16561 caaatcaagt atactgtcca gcacttcttc catagcatca ggttttacac acaaaaaaac
    16621 aatatcacat tttgtaataa cctcttcaac actatcagct aaaaaggtgt tcttgtcaat
    16681 aagagactta gccttatctc tatatttctc gtatattaag atttttgagc cactttcttt
    16741 tatgcctctt ataagggaag aacccatatt tccagctcca ataaaaccta gaactttatt
    16801 cattgctcac ctctttaatt attatggtat ccttttcaac tgggatatct gtttttatat
    16861 aatataccat accaggatgg tttgctttat caataggatt catatcttta tcataaagca
    16921 tacttacagt ctgtctgtaa tttttaaaat gaggagacat aagaataatc tcatcattaa
    16981 tgaatactgg attaatctca tacacttttg ctaaaaatgt cttttcatca tagttttgca
    17041 ctaatcctgt atatgtatga cttctgatgt aactggaatc tgaataaatc attgaatctt
    17101 tggtcttttg tatgtaatcc ttttcatcat ttatgttata tgcagtagta aactgcctgt
    17161 ggctaggcat gctgactatc tggttatagt actcaatatt tgaataatat ttttcaggat
    17221 tttctaaata atcatcaaaa gcagatctat aagctttaca tactgtcgct acataaaatg
    17281 aactcttcat tcttccttca attttgaaac tatcagcttt actctcaagt aactgaggta
    17341 tatgttttat gagatttaag tctttcgaat ttaatatata tgatcctgaa ttatcttcat
    17401 aaacctcaaa atactgacct ggtctttttt cttccattaa tgcatatttc catctgcaag
    17461 gctgtgcaca gtcaccctga ttagcatctc ttccagccat atatgaactt aaaagacatc
    17521 tgcctgaata ggatacgcac attgctccat gtatgaaaat ctctatctca tgttcttttc
    17581 caactgcatc tttaattctt cctacatcat ccaaagacaa ttctctggca agaatcagcc
    17641 tttttgctcc aagtttacaa aaaactttag cactttcaac attcaatata tttgcctgtg
    17701 tacttacatg tatatctata cctttcgcat attttttagc catctcaata agacctatat
    17761 ctgcaataat caaagcatcc ggtttaaatt gagttaattc tgacatagtt tttgcaatgt
    17821 cttcaatatc tttgtttttt ggaagtatat taagagtaac ataaattttc ttatttagct
    17881 ctttagcata taatattgct ttttccaaat catcatagga gaaattgtca gctttatctc
    17941 ttaaactgaa tctgtttaat ccaagatatg cggcatctgc accataagca aatgcaaatc
    18001 taagtttttc aatattacct gcaggagcca gaagttctat ttttttcatt tcttaaccga
    18061 tatcgacaat ccgtccgcta tggataatat cgatgtatca aacctttcat ctttggttat
    18121 cttatcgaga aagcttctta atctttccac caatgtagct tgtctgtgcg gaatcttgtc
    18181 ttttttagct accatccccc gataaagaac attatctgca attattactc ctttttcatt
    18241 cagaagattt gaacaaatat caaaatattt ttcatattgt ccttttgcag catcaataaa
    18301 tataatatca tacttcttat ctatgttcat tagatagtca taagcttcag cataaataat
    18361 attaattgtt ttttcaaaac cactattttt tatgttgctt tttgctattt taaccatatc
    18421 aggatcattt tctacagtat caatataacc agatttgtct aaatatccag caaaaaatat
    18481 acttgtaaat ccaattgcac agcctagctc tagaaccgct tttggttttt ttagattgaa
    18541 tataacctca agagcccgta tagtttctag ctttgcaacc ggtatattat tttttttagc
    18601 gtgctcagac atttccttta aaaaaccttc taaaggtttt aatgtgcttt ggatatattc
    18661 ttcaacatcg atatttacaa tattaaaacg cttttccatg ttattttgag tataacgata
    18721 cattgtaatt atgctgagct aatgtagtag cataagcatg tgcatcagta ccatcattct
    18781 tagcaacata atagtaatag taatgagttt caggatacaa agcagctgca atagctgaca
    18841 atgaaggatt acaaattggt cctggaggta gtcctttata catgtatgta ttgtaattat
    18901 tcatgatatc atgatcttca tatgtaagga attcttttac agctcctgtt tcatttatat
    18961 acagatactg aatagtagca tcaatctgca gataattcat tgtagggtct tctgatttaa
    19021 gcctgttata tataactcct gcaatcattg ccctctcatc cattgatgaa gcttcttttt
    19081 ccagtaatga tgcgattatt agtatttcat caatagtaaa acccatctct tcagcttttt
    19141 gacgatattc tttattaaat ttggaatcaa aattatccaa catctttttt actatttcac
    19201 tctcgtcaga tgtcacatca aaaatatatg tatctgggaa aagataccct tccagtctgt
    19261 ttgttcttct cgagtcttta ggaatctggt ctatgaaatc atattcaaat tcaaaattat
    19321 tacatgtatc gtaaaaatcc tgtgcatctc ctacaccatt ttctgtaaac agatttactg
    19381 tctgtgttat tgttaaacct tctttaaaaa atatcttctt tgtaggatta gcaatgctgt
    19441 cttgcgataa aatgtgaatc agcatctcat agccttgagc cgtaataaga tcaatctctc
    19501 cagaatcata tgtgacagat acatcaaaaa tatgcttccc agacttatag ttaccttcat
    19561 tgcccattaa aagagaataa acccaaaagt aggtatcatt tgtgataact ccttcgtcca
    19621 ttaatatttt cgcaatatct tttgtagtag atcctttcgg aatatcaatg accttttcgt
    19681 tctcttctgc aataacacca atagcttctg ctgcattaaa tgaatttgcc tgttttacca
    19741 gtgtatttgt aaatgtataa cctttgtaaa atacaaaacc taaggcaaat aagaaaacaa
    19801 gccaaatcat taatgtaata aattttctaa actgagattt tggtttatat acaactatct
    19861 ttacaacctt atttcttctt ttttctttcc tttttgctat ttcttctttt gaaaagatat
    19921 tccatatccc atccaaaaac atcagaaact tatttgtttt cttttcttga aaatctttgt
    19981 cttctatata attatctaca tcagaatcat cagcaatttc ttcatcagtt tcctgattgt
    20041 ctgcatgctc atcttgttgt acaacatcag attcatataa agcatcatta tcttcgatct
    20101 cttcaacttc aatctgatct gtatgtttta taatatcttc gtcagctgat tcagcctcat
    20161 cctgcttttt attaaaaatc ttagtcaatt aacactccat aaacttcaat cttttacata
    20221 ttaacataaa atcatcaatt ggtgaattat ttcttttatc ttggatcttt accgccaatt
    20281 tcaaaaaatc tgtttctagc ccaaccagcc cattcatctt taatacgtaa tgtattataa
    20341 tcaccataat ctttaaccat aagaccacct tttttatgaa aaagatcaac catctggtct
    20401 gcagactttt caataagctg tttgtttcct gtaggtaata cagcctgaat atctacagga
    20461 gaatataaag tagcttttaa atttatcttt tcagctagtc tttctattcc cattaatgtt
    20521 ggctggtcaa gttggaatgc gtcaactccg gcatcaagaa gatcatcaag aatttcatat
    20581 ataaaaccac aggaatgcat taatactttc aaattgtttc tatgtgcagc tttacatagt
    20641 ttgacatatg caggtttgaa tattttattc cacattgctg gtgaaacaag cagtctgtct
    20701 tgtgttcccc agtcttcaca aaaaaatatg ccatcaacac atttcataga accatataga
    20761 tctaccatgt taacaagttt actgattacc atatcttgaa gtttaagaac gttttcttcc
    20821 tcaagtatga ggtcttcaag aaaaacctcc attttacgta catttctcat aattgcaaat
    20881 gtacatcctg gtagtggacc aagaatgaat ctttccttat atggttctac acttaaatga
    20941 gcatctttaa atctagtttc atcatcaatg ttaggtagtt tatatgatga caaatcatcc
    21001 catgattcca gcccaccttc cagtacttct cctcctccgg tagtgtcatg ccacatctta
    21061 ccccaaaggt tattatattc atcaagatgt aaataacctt caaaattctt atatttagga
    21121 tatttatctg caaaaaactc aggtttctgc catttgtgtt cttctttttt gggtatatta
    21181 agttcaacat attttatatc tcttattgaa ccatctaaaa aatcatatcc aattctttct
    21241 ggattatcaa actctaaaac tttttttact atcgtttttg atatcatcac ttgcctcctt
    21301 gaaaattata tcattaacat ttatttaaaa tatttaacac ctgattcaaa cagcttttgg
    21361 tctttattgc ctggaacatt aatacacaca ttgttaccaa atctttctga atgtcccatt
    21421 ttacctaata ttctgccatc cttgcttgta acgccttcaa tagcataata tgagccattt
    21481 ggattatatg caatatcata tgtaggatta cctgaatcgt ctacatattg tgtagcaatt
    21541 tggccatttg cactcaataa attatactct tgttctgtac aaatgaatct tccctctcca
    21601 tgactgactg gaatagtgtg tatatcattt agattaacgc aggagaacca aggtgataac
    21661 tttgatatga tttttgtatt caccatacat gaaacatgtc gtccaatact gttatatgtc
    21721 aatgtagggc ttttttcaga cagttcgctt atcttcccat atggaatcag tccaagtttg
    21781 acaagagcct gaaatccgtt acaaatacct aacatcagtc catctctact atacagcatg
    21841 ttatgaaccg cttcttttat gtatggattc ctaaatactg ttgctatgaa tttgccggaa
    21901 ccatcaggct catcaccagc agaaaaacct cctggtattg ctataatatt ggaatcattt
    21961 atcagtttaa caaactcctt aactgaaaca tcaatatcat ttgctcttgt gttcctaaat
    22021 attactgttg agcaaattgc accctggtcc tcaaatgctc tttttgtatc atactcacaa
    22081 ttagttccag gaaagacagg tatgaatacc cttggttttg ctatgtttat cttgctgtga
    22141 aagacttttt tatattcata tggtttattt gttatattag gcttttctgc tattgattta
    22201 gttgaaaaaa cagattcgta ttttgatgta tattctgtaa gtaaagtttc aagatcaaca
    22261 actatatcct tcataatgaa tttactctca tcaatagtct taccaataac ttctaaatcc
    22321 agattatcat atctacttaa gaaatcatta atgtttgaaa ttttctgcga atcaatctct
    22381 ataagaatat cacaataacc gttatcaaaa agtgactctt catttgcagt gagtacaaat
    22441 cctatcatat ttccaaaaca cattttaagt agtgcttcag cagcagactt atgttttatt
    22501 gtggaagatg aaagaataag tgaattacta ttaaattctt gaagtaaatc aaaaatcttt
    22561 tttgcatatt gaaaatcagg agacatatca gcatttcttc tatgtcttaa tcttaagata
    22621 tagttatctg tttttttaaa ttcagaagat attatatttc ctgctgatgt atggctaact
    22681 gcaaaactaa ctaatgtagg aggaacatcc atatccataa aagatcctga cattgaatct
    22741 tttcctccta tagctggtat accaagctca gtttgtgcat aaagcgcacc tagcaatgcc
    22801 tgaaaaggtt ttccccatct tttagcgtct tttcttaagc tttcaaagta ttcttgtaaa
    22861 gtaagacggg tttttctcca ctcgcctccg acacttacta tctttgatac cgaagacaga
    22921 actgaataac aagcaccagt aaagcaagac cattcagata tatttggaaa aaatccatat
    22981 gacataactg tacaataatc agtcttctta tccaataccg gaagtttggc aaccatagct
    23041 tcagatggag taatttgata ctttccacct aaaggcataa gtactgtatt agaacctatt
    23101 gtgctgtcaa acttttcgat taagcctttc ttagaacatg agtttatatc attgactgat
    23161 tcaaaaagct gtttttcata tgatttatct gatttgttta tgaagggatt tgatgatgag
    23221 tctttcttag tgacatgagc tttcgcatgc tgcgtgacac cgttagtatc caaaaagctt
    23281 ctttctatat tcacaataac ttttccattc caaatcatct taagcttttc tgtatcagtt
    23341 acctttgcga cataagttgc ttcaaggttc tcaaaagctg caagttcgaa aactttatcc
    23401 atatcacttt tatcaataac tactgccatt ctttcctgag attccgaaat agccagttct
    23461 gtcccatcta aaccttcgta tttttttgga acattatcta aatatatttc caatccgtct
    23521 gcaagctctc cgattgcaac agctacaccg cctgcaccga aatcgttaca tttttttata
    23581 agcttgctca tgtcaggatt tctaaataac ctttgtatct ttctttcagt aggagcatta
    23641 cctttttgca cttctgcccc gcattcagat aaagactcct cagtgtgaac ctttgatgag
    23701 cctgttgctc ctcctaaccc gtctctgcct gtccttccgc ctataagtat gattacatca
    23761 tcggtacatg ggtcttctct ttttacattt tcaattgcag cagcagcaat aactgcaccg
    23821 atttccattc tctttgctat atatccattg tgatatattt catcaacctg accagttgcc
    23881 agtcctacct gattaccata tgaactgtag ccgtttgcag cttctcttat aatctttatc
    23941 tgtggaagtt ttcctggtaa agtatcttca atttttgaaa acgggtctgc tgctccagtc
    24001 actctcattg cctgatacac ataacttctt cctgatagag gatctcttat agcaccacca
    24061 agacaagtag ccgcaccgcc aaaaggttca atttcagtag gatgattatg tgtttcattt
    24121 ttaaacataa taagccattt ttcatcgcct ttgcttgtag gtacatcagc gacaatcgaa
    24181 catgcattat tttcttttga ttcttccatg tcatccagaa gacctttgct tttaaggtat
    24241 tttgctccaa tagtagcaat atccattaag cagattggtt tatctttatc tttataaagt
    24301 tcttttcgaa tttctatata tctttcatat gcttttttga ttttgtcatt aaatatgtct
    24361 tcatctatta ctacttcatc caagacagtg ttgaaagtag tatgacggca atgatcagac
    24421 caatatgtat ctattacttt gagttcagtt atagttggat ctctgtccag tgacttaaag
    24481 tattcttgga caaataaaag gtcgtcttca ctcatagcaa aaccacactg gtttttcatc
    24541 ttcacaattg catctttgtc agactttata aatcctgaga cattttccac catataagga
    24601 acattataat gtgatttcag attatcaggt ttttccatgc ttgcaagtct gcactcaact
    24661 ggatttataa tatattcttt tattttatca atatcattat ttttaatatc accaaagata
    24721 catactaact tagctgtctt aacaataggt ctatgatgag atattagttg tatgcattgt
    24781 tcacaagaat cagctctttg atcatactga ccaggaagat attcatatgc gaatatatgt
    24841 tcgtacctat taaaatcaac agcctcttca tatacattat cagtctgaat ctcagcaaaa
    24901 atgccgtatt ttgctacggc atatatgctg tcatctaatc cttgtatatc atatctgttt
    24961 aaaatacgga tacctctaat accctcaata cccaaaagcg ttacacattg ctcatgtagg
    25021 cgctttgcct ctaaatcaaa tccgtctttc cgttctacaa atatccttct tacattattc
    25081 aatatatctt acgctccgtg ttattttaat taaagctatg tatataatac tatattaatc
    25141 aactgacttg ttaagcattt cgttatttct tctcaaaaaa tccgtcatgt cttctttttc
    25201 gagttttcca gctgaaagca ttgctaactg atatatgtac atagcaacat cctttttttt
    25261 gtcttcatca gtcatctcca taagcttttt aactactgta ttttgagtat tgagcactag
    25321 aatatctgtt gaaggaatat ccattccata cattgtttgc atactcttca ttctgttctc
    25381 atattcattt gcactaataa tggcaggaac atcagatgtt tttaatgatt ttaactccac
    25441 agtaacattt ttattattaa gcatatcttc aaaatatttc ttaacatcat caaaaccttc
    25501 ctctttagta tcttcaccaa gcacttcttt tataccgctg tctataccga taaatgatgt
    25561 acctgtgttt tttgattcta taaaggaaat aaaatgagaa tcaagaatat tatcaagtat
    25621 tacagcattc aaatcgttct ctttgaacat attaatatac tgggcctgtt tttgtttatc
    25681 agaaacataa tatattttat ctttctgttt tttatcttca gataccttct tatattcgtc
    25741 tagtgtataa tacttgtcat aaatatcttt gtatactact gaatccttta ccttatcata
    25801 aaacttatct tctttaagac aaccatattt gacgaataca ttaatgtcat cccagtattt
    25861 atagtattgt tctgtatcat ttttatataa ttgtttaagc ttatcagaaa ctttctttga
    25921 tatatgacct gcaatttttg tggaatatcc atcattttgc aaaaagcttc tcgacacatt
    25981 caaaggaaga tccggacaat caataacacc tttaagcaga agcaaaaatt caggtattac
    26041 ttcttttata ttatctgcaa catatacttg attacaataa agttgaatct gaccttctag
    26101 tgtattgtat tcattagtaa gttttggaaa atatagaatt ccttttaaat ttaccggata
    26161 atccatttta agatgaatcc agaataaagg ttctttaaaa tcatgaaaaa cttccctgta
    26221 aaattcctta tactcctcat ctgtacagtc tttagcatct tttaaccata aaggagatgt
    26281 gttatttatg ggtttttctt cttctttttc ttgtttatca tctttctttg caccttcttc
    26341 agaaagataa atttcaatag gaaggaactt acagtatttc tccaaaattt ctctcatctt
    26401 ccaaacattg ataagatcct tgttttcctt atctgaatta atatgaagaa ttatgtcagt
    26461 tccatgaaac tgcctggttc cttcattcat ttcataacct gaagcaccat cagatttcca
    26521 atacacagct gattcgtctt taagatatga ttttgtctgg atttcaacta attcagatac
    26581 cataaatact gaataaaaac caagtccgaa gtgacctatt atttgatcct gagcatctgt
    26641 tttgtctttg tatttttcaa aaaagtcaat tgcaccagaa aaagcaatct gatttatgta
    26701 ctgtttgact tcatcaaagg tcattccgat accattatca gaaatagtta tagtcttatt
    26761 ctttttatca acctttacat caattctgta cttatcaggc ttctcaactt cacctatagc
    26821 gactactttt ttgtgttttg ttatagcatc tatcgcattt gatactactt ctcttaaaaa
    26881 gatatcctta tcagaataaa gccatttctt tattattggc aggatattct ccgtattaat
    26941 tgatatgttt cctttttcgt tcatcttata cctccatata aattattcaa caattttttt
    27001 tatataattg aatcatttaa tatgattcat actttctaaa tatttatagt tcataaaa
//
LOCUS       DAOSXM010000022        27000 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2111
            NODE_1136_length_27000_cov_11.951865, whole genome shotgun
            sequence.
ACCESSION   DAOSXM010000022 DAOSXM010000000
VERSION     DAOSXM010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864178
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 27000)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27000)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:24:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,261
            CDSs (total)                      :: 2,229
            Genes (coding)                    :: 2,218
            CDSs (with protein)               :: 2,218
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 3 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27000
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1136_length_27000_cov_11.951865"
                     /isolate="AMR_MDS_2111"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..474
                     /locus_tag="PLQ68_04730"
     CDS             <1..474
                     /locus_tag="PLQ68_04730"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXK71991.1"
                     /translation="TGCYPSKPIYEGTKEVDFDHTCWLIDDITIELIADSKGIHVRED
                     MLKLIYKIKGRDRLVLVTDSTNFTKDNNSTDVNILNGTDLAGSALTLNKACFNMMKHT
                     DISITDAFYMASYMPARLLGIDDIKGSIEPGKKADLILVDDLMKIYEVWLDGQMI"
     gene            complement(475..804)
                     /locus_tag="PLQ68_04735"
     CDS             complement(475..804)
                     /locus_tag="PLQ68_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009005054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK71992.1"
                     /translation="MTKVVIDPGICNLITSVEAVSEDGMEVTLKVKSGCVSINEMFKK
                     IGNTFDAYELCLAKPGDGPLYEYAKENLPGHGGCPVIAGIVKAAEVECNLALPKDAKI
                     TFVNGKE"
     gene            962..3235
                     /locus_tag="PLQ68_04740"
     CDS             962..3235
                     /locus_tag="PLQ68_04740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK71993.1"
                     /translation="MGRFTERISLRLIPLPKEMSCNDIKRVAFSDVKIDVIFPEDSTL
                     LSAKRKLITYFGNSIYGSFRIRIVDPMPNTVKVLNNFPNSEQAYHMWYDGAINISAIT
                     SKGIMNGVRTLLQAVSKEDNEFIIPLLFIYDYPDIPLRGQWGGDSMDDIEFTSSLKLN
                     SLDYTGSFDINSENIGTGYLEKKEVLENCIKEGIVLTAYVPHLENVIGRYLNKPEYKK
                     LIEKIKNVPLPESAQRNDYKPGLCVSSEHTKKLVYEWMRELAQIKGVKSVMVWLSENT
                     EACICEKCKDQEPFILETKMLVELFEKLKKEFPDIGCEILLTQGSYSVNEKILEIVPE
                     YISVLYYHGSRTYISDKNPMIYEGLMPYIERGGKLGVYPQITHGWRCVFPWTGPDFIK
                     YRCDEFVEKNIHKVVGYAVPSNVYHRFNIAAMAEWTWNNKGRDEESFMKAYAAINGID
                     ADGFVKWCNLNMGPAWALAENRFLVGQMFNFPVIMKSKFDMEDFRFTDYTKKPIKDID
                     KYIRLADRAVKAAKQIGYTQGELESRCIVDVLRCAGAYQKFFTLLNNKKRTHEEDHQM
                     SLMYEMMHNNSKNIYHALLDWKELIDKDNSHFQTRITDTAVICMRITDFMKNILNKLE
                     ISHKEHHSLEYSELGRWDESVFDKASLTTIPYEISKFTDKDGRYHICFDFVDSKIGTL
                     IKDISVMQNGKCISKIVYDAKVPFMKRINIYEPWYEALVDVSGIEKTSDRTLEVTLEK
                     PDEQKATCRGILGIRKI"
     gene            complement(3236..4264)
                     /locus_tag="PLQ68_04745"
     CDS             complement(3236..4264)
                     /locus_tag="PLQ68_04745"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HXK71994.1"
                     /translation="MNSRERVNSAIHFKPVDKVPLQYNYTPVGYFEHGDKLNELYKAY
                     PDDFGPFEYKQVPVLTKNQLDENGNYHEFKKDEWDTVWEYRIYGIAGIPHIYPLSDMD
                     KLKDYIVPSHPDIGSEQFLIDKQQAVEHKKNYYLSRFAGTIIEKLKALCPDENVYCDM
                     AEGTKEINQLADMIVEYYEKKIQYLLAIDVDGFYFGDDYGTEKSMLLSPSLWRSFFKP
                     RWERLFEPIKKAGKNIHFHSCGYVLPILEDLKELGVHSIWPQLPAYDMVEFAKYCRSL
                     KLAVAIHTDRANIMTFGTPSQVKDLVKKEYEVFRMQEGGSWFYVEADNGFPFENMEAL
                     VETIAQYR"
     gene            complement(4366..6111)
                     /locus_tag="PLQ68_04750"
     CDS             complement(4366..6111)
                     /locus_tag="PLQ68_04750"
                     /inference="COORDINATES: protein motif:HMM:NF019313.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase fold protein"
                     /protein_id="HXK71995.1"
                     /translation="MSKMGYTDKPVEKLTEDSFGIKTYINGLCQFIVSCDTPMTISIQ
                     GDWGSGKTSMMSMIKKEIDDKVYPIWFNTWQYSQFNMGKDLCVSFLENLITKLHSKAE
                     NRTTKILETSLRILKIGAKTAISTVGLDGGAIVDVFNKEDADIAKAVSDLKDAFQDSV
                     NDKLKESKKDRVVIFVDDLDRLNPAKAVELLEVLKVFLDCENCVFILAVDYSVVTAGV
                     KQKFGNLVDDDKGKSFFDKIIQLPFKMPVAQYDIKKYIYDMLVKMEMKVNSKIISEYK
                     DLIKTSIGFNPRSIKRLFNTYQLLNLISCCKASKVQEELRQKLLFATICMQMTYDHLY
                     SLIVTSSKNNELTGKTLRSLADPEIVRNYKGLPEELRLVDEEEKIDEDELLRMCSFMK
                     AFVSVVDIDKSMLLSDQELNDLKEILFFSSVTSIMEDTKVNTGMEIEQKIRTRNRAII
                     DAVDANLTNTVGDFMIWQPIKAENDIKLSDVSCSLDLLSTSGVPFTFEFLLTSKSLED
                     ICLHVRFKCSVEFSEKLKELFGPNPLDYEIIPVINEKGYEYTCIGIKETDAAVTVTRK
                     IEEVYETLKKKGYIS"
     gene            6344..6805
                     /locus_tag="PLQ68_04755"
     CDS             6344..6805
                     /locus_tag="PLQ68_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007059058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HXK71996.1"
                     /translation="MKHIEIGKKAPEFILEDIHENQVKLSDYKGKKVLLSWHPLAWTG
                     VCTDQMRALEANHERFEKLNTVALGLSVDPVPGKLAWASVLRIKNTPLLSDFWPHGKA
                     AKDYGIFLEDMGISERANVIIDEEGIVRWMKIYPIAQLPDIEEVFKKLEEI"
     gene            complement(6865..7680)
                     /locus_tag="PLQ68_04760"
     CDS             complement(6865..7680)
                     /locus_tag="PLQ68_04760"
                     /inference="COORDINATES: protein motif:HMM:NF021873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HXK71997.1"
                     /translation="MKIFAIRDNNDSANKDIAFLIYYECDKRFYIELPENADPWETPL
                     PLSSFLKRGETTVNAYWSRIWVQQRIIPSDRQNIGQILKDNKLDSYDEFDLLMLTNGR
                     CAQDDYYLVSVSEKDFPESFFKRFQKKIEDVVPLTNGHLLIFFRDGNVKKCNMKTLLI
                     DNKAFLPILKDEKMFYRVSMQSGGYGVCWGENLNIADYILYDCGKDIPLSLEDFRLFV
                     ENRVINTTEAAELLNCSRQNIDDLIRRKKLHPLKTTQKSKLFLKSEIIQRNWK"
     gene            complement(7655..8479)
                     /locus_tag="PLQ68_04765"
     CDS             complement(7655..8479)
                     /locus_tag="PLQ68_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008708678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK71998.1"
                     /translation="MQTKVILEKQDLTYLSWTKIQNMSGAVGSFLKAYSDLGGDKVYY
                     KLSDYDAYKGITGHECINELIADRLLTILDVEHLNYQLIHADILIDGQLHETCLCASS
                     DFKKRNESKIALDTYYQAERETGEKPLDFCLRHDWSEYIWKMIVVDFLILNRDRHGAN
                     IEVVRNSKEKTLRLAPLFDHGLSLLCSCKNEDAVHVFDIMADKPVQCFVGSSSAKDNL
                     NLIPKNSIPQFNPLKESDYEILFANLSTVIPQAYIDKIWEMLWRRWMFYEDFCNQR"
     gene            8873..10210
                     /locus_tag="PLQ68_04770"
     CDS             8873..10210
                     /locus_tag="PLQ68_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005840515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXK71999.1"
                     /translation="MHIQRDYLLEKLKAGMKSPLIKVIIGVRRCGKSYLLFNIFYDYL
                     KSINVREDHIIKIRLDELKNAALRNPVKLSSYINERILNDGEKYYILLDEIQECVKIA
                     NPDFLDNEGNLIEGISVDESYLTYHDVLNELLNIEYVDCYVTGNNSCLLSSDLPTEFR
                     NRNYNIEVFPLSFKELHQAFPDMQYDELWNEYITYGGMPLAVLNRSVQSKQNYLKDLF
                     EGTYFKDIKERYGLRDEASIDDVTDIVASCIGSPLNPGKIENTFKSRKSSNITDNTIS
                     NYLKYLENSFLIKEAKRYDVKGRKYIGTLSKYYFTDIGLRNARLNFRQIEESHIIESI
                     IYIELVRNGYSVDVGQVESWIQDPDTKKRCRVNYEVDFIVNEGNRKYYIQSAVELSTI
                     EKVKKEQKSLMKIDDNFRKIIIVKGFRNPLYNDDGILVIGLYNFLMDVKIIEQ"
     gene            complement(10326..11432)
                     /locus_tag="PLQ68_04775"
     CDS             complement(10326..11432)
                     /locus_tag="PLQ68_04775"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HXK72000.1"
                     /translation="MNINNFHVLIKPASSLCNMNCKYCFYNDVSNNRNIKSYGIMSYD
                     TSYKIIDRALEVCTNGEIGFSFQGGEPTLAKLDFFEDFVKYAQKVKTPKVKYTYTIQT
                     NGLLIDEQWCRFFKSNDFLVGVSLDGIKEIHDELRIDNNKKGTFNRIMNSIRLLKKHE
                     VPYNILTVITKYNARHPQALWNMYEKNGFDYVQLIFCLSPLNMPYLEDLYTLEPKAYA
                     EFLKTFYDIWLDKYNQGKYISVRMFDNLIKMCAGYPAEQCGMMGFCQAQFVIESDGSV
                     YPCDFYVLDQYRGGNIHNDSFESIFKSDNFLTFLSDNSTKNSMCDTCEVKSFCNGGCK
                     RYRYFYFQEEGYCPQKDFLIHAIPSMKQIAASLW"
     gene            complement(11429..12910)
                     /locus_tag="PLQ68_04780"
     CDS             complement(11429..12910)
                     /locus_tag="PLQ68_04780"
                     /EC_number="3.1.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005944937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HXK72001.1"
                     /translation="MERPNIILIMCDQFRGDCLGFDGHPDVKTPYLDTLASQGIHYRN
                     AYTACPSCIASRASLHTGLSPKNHGRVGYQDRVEWNYPQTMASVLSDAGYYTQAVGKM
                     HVYPIRNLIGFHNVILHDGYVHSYRNTSMPSYEDSRFADDYFYWFKDKMGISRDITDT
                     GLESNCWMTRPWPYEEYLHPTNWVTDQAIDFFRRRDRSKPFFLNISYVRPHPPFDAPK
                     AFLDMYSNKNLSEPITGDWDDFDLLEKEGRVYNSQSGPIDKEQIRMARAGYYACITHI
                     DYEIGKLLEAMHQDCLMKNTIILFTADHGEMLCDHMRYRKAIPYQSSARIPMILYGPE
                     KCIKDTHKRSDKLVELRDVMPTLLDIANIEIPDNIDGKSFLPGNESNKSEYIHGEHSY
                     GYYSNHFIVTEYDKYIWFSQSGIEQYFRLDKDPTESHNAINDAEHADRIKYLRNILIK
                     ELEGREEGYTDGNVLITERPTQKYRKSVLEDFKKQYEEVNKGY"
     gene            13055..13705
                     /locus_tag="PLQ68_04785"
     CDS             13055..13705
                     /locus_tag="PLQ68_04785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72002.1"
                     /translation="MKKILCILLIVSMMLILCACDKTQCETPSGIYSTKDNMSNIHIY
                     SDGTFTIFHIISSRIITGEYKAEEDLLVLTDLENDVYKFNIEKDRLIFLSTTAKYTYF
                     EEDMTYYAKQIDYENIQAAVYYGFSSYALFTDNDDVVKELYDGYYNMKTQETDEELDF
                     PSAFLVVVGDVSFFMDKNGCIRLNDNDYIVKDTANSISYARLKEIYNDKSLMMGED"
     gene            13760..14098
                     /locus_tag="PLQ68_04790"
     CDS             13760..14098
                     /locus_tag="PLQ68_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72003.1"
                     /translation="MKKIAFFAFRGNEMCFIHILLNALEMKQKGKDVKIIFEGEAVKL
                     PKILSDKENPMYKKCIESGIIDGVCEACSKALGAYEDNKALGLNFLSELKGHPSMDRY
                     MEEGYEIILM"
     gene            complement(14228..14303)
                     /locus_tag="PLQ68_04795"
     tRNA            complement(14228..14303)
                     /locus_tag="PLQ68_04795"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14268..14270),aa:Thr,seq:tgt)
     gene            complement(14309..14384)
                     /locus_tag="PLQ68_04800"
     tRNA            complement(14309..14384)
                     /locus_tag="PLQ68_04800"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14349..14351),aa:Val,seq:tac)
     gene            complement(14533..15729)
                     /locus_tag="PLQ68_04805"
     CDS             complement(14533..15729)
                     /locus_tag="PLQ68_04805"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004294157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HXK72004.1"
                     /translation="MKSLKELYRIGRGPSSSHTMGPDRAARRFKEEHPDADEFTATLY
                     GSLAKTALGHMTDKAIREAFSPKNVDITVDQFSEDIPHPNTMDFMAIKDQIVIDKWRV
                     MSIGGGAIRIEGKPLMDTSNVYKEENFTQIKQYCEQNDMRICDYVIKTEGEDICDYLE
                     TVWQQMKLSIKEGLNATGILEGGLQLQRKAKYLFSQRHMDESTSTKENRIVCSYAFAV
                     SEQNAGGGIVVTAPTCGACGVLPAVLKYMQDRNGFSDKDIIKALATAGVIGNVIKTNA
                     SISGAECGCQAEIGSACSMAAAGLAELFGMDLDQIEYASEVAMEHHLGLTCDPIMGLV
                     QIPCIERNAVAAMRAINSVSLANFLTHTRKISFDMVVDTMYETGKDLFSKYRETSEGG
                     LASKYK"
     gene            16068..16895
                     /locus_tag="PLQ68_04810"
     CDS             16068..16895
                     /locus_tag="PLQ68_04810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72005.1"
                     /translation="MKNKTAIKTINWLVPIFIVGLIIFNSTTEALVLAVAYFIYRVII
                     ELSRIYSYIAIKNYSKGNLIKARKYYKKAYEAKVKELPIVASYAYLLILLKEYEKAEE
                     VLNELEQMKPEGKSMTSLMLCRAVLAWKTQKNLILALSKIEAMDETMRNQWYYNVYAK
                     LCIFSEDVEKAKQAAIKGYEYLKTNPVAIENLLIIHCINEDYDKALSAAQKLINGKKN
                     MKPSSRDAYYYCALAYEKNNDTDMAAKLYVKALEYEDTSMTYADNQQVIRKAGEYMK"
     gene            16892..17800
                     /locus_tag="PLQ68_04815"
     CDS             16892..17800
                     /locus_tag="PLQ68_04815"
                     /inference="COORDINATES: protein motif:HMM:NF014627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HXK72006.1"
                     /translation="MKTSMNKIWPKVFDYFIITIGVLLSAAAVNLFFIPYKIHSGGVS
                     GIATVLYYIFKAKIPVGVLIVALNLPLFLVGLKVIGKTFMARTLYATLAYTVAIDLTA
                     PWLTALSNEITIESNNIPDLMLFCLFGGAIMGFGLGMVFTRNSCTGGSDLFAEILRKK
                     GIHLSMGTLMFMFDVISIVMSLIVFKNLVLVLYSILAIFISMKVIDLILDGANVAKAV
                     FIMSDKSEQIADAIIKVLGRGVTGLSGRGMYFKRERETLFCVVKNRQLPALKKLVKQM
                     DEKAFLIITSAHQVLGEGFGAFDKEL"
     gene            17901..18743
                     /locus_tag="PLQ68_04820"
     CDS             17901..18743
                     /locus_tag="PLQ68_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015908416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HXK72007.1"
                     /translation="MEQKLLLSLKRKAAEVRKNIIEEVFSANSGHPGGSLSCTDILTV
                     LYFKHMNVDPKKQKDPDRDRFVLSKGHASPVLYSILAEKGFIPKEDLVTFRQADSYLE
                     GHPSVKDVPGVDMTTGSLGQGISAACGMAQMGKLDKKNYRVYAILGDGELQEGQVWEA
                     MMYAAHYKLDNLTAFLDHNGLQIDGDITKVMSPEPVAAKFESFGWNVIIVDGHDMTQI
                     DNAIEKAKDTKGKPTMIVAETIKGKGVSYMENQYGWHGKAPNQELRDKAIAELDAYIE
                     NLGV"
     gene            18745..19680
                     /locus_tag="PLQ68_04825"
     CDS             18745..19680
                     /locus_tag="PLQ68_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003442916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HXK72008.1"
                     /translation="MKKATRAAYGEALAEYGSNEKIIVLDADLSKSTMTASFKELYPE
                     RFFNTGIAEANMMSSAAGMATCGKIVFASSFAIFAAIRAGEQIRNSICYPKLNVKVAA
                     THSGISVGEDGATHQAIEDIALMRALPNMTVIQPCDGRETKQAVKAIIDYDGPVYLRL
                     GRLAVEDIYDNSYVFQMGKGVQLKDGCDLTIIATGLMVKPALDACEILKTQGILARVI
                     NIHTIKPIDKNILIKAARETGAIVTAEEHNIIGGLGSAVCEVLGEEWPVPIKRVGIED
                     KFGKSGKPEELLKMYGLTAENIVIKSKIAMALKKK"
     gene            19677..20471
                     /locus_tag="PLQ68_04830"
     CDS             19677..20471
                     /locus_tag="PLQ68_04830"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HXK72009.1"
                     /translation="MIFESHAHLLSNKFENDRDEVIKRALENGVYAVLNVSDDLKTSV
                     EAVKFSKQYNNIFSSAGIHPHNGMDFKDDDLIVIKQLALDSKTLAIGEIGLDYYYDFC
                     ERNVQKEVFVKQLELAKSLDMPVIIHDREAHKDTIDTLNLFKGNLEGVIHCYSGSAES
                     AKILLDMGYYISFTGVITFKNANKTIEALKAVPIDRLLLETDSPYLTPEPYRGKRNEP
                     KNLVYIAKMMAQVKQVSYEFLLEKVWENTFELFKKAKPYRKVLEEK"
     gene            20468..21307
                     /gene="rsmA"
                     /locus_tag="PLQ68_04835"
     CDS             20468..21307
                     /gene="rsmA"
                     /locus_tag="PLQ68_04835"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HXK72010.1"
                     /translation="MKNVDNIIPLPNYVRAKKALGQNFLIDKTIALQITDALEMNEDD
                     TVIEVGSGTGALSSLIAPHCKKLYAIETDQDLIKPLSATLSDYSNVEVVNKDILKIDI
                     NTLINTKDGYKTVANLPYYITTPVINKFIEQEHLPDVMVFMMQKEVADRILEKEGSRI
                     YGSFTIYVNYRYAASFVAHVPPESFIPSPKVTSTVLKFIKRDKPLVDVPSEEFFFSVV
                     RASFSQRRKKLSNCISSMSSFNISKEDVEKILSDMGLRADIRAESLSIQKFAELAWKL
                     HSI"
     gene            21509..23851
                     /locus_tag="PLQ68_04840"
     CDS             21509..23851
                     /locus_tag="PLQ68_04840"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008816017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside triphosphate reductase"
                     /protein_id="HXK72011.1"
                     /translation="MIMKITKRDGREENFSPLRITRAIFLAAEAVAKEENTKADFTIA
                     EELTDKVIQLLNRRFIETIPTVEDVQDAVIKVLIEEGHARTSEKYIIYREARNKARSM
                     KTRLMRAIEEITFHDANQSNLKRENANIDGNTAMGTMLQYGSTVSKEFCKTNLLDPLH
                     AKMHEEGDIHIHDMDFYNMGTLTCCQIDLTRLFKKGFSTGHGHLREPQDIGSYAALAA
                     IAIQSNQNDQHGGQSIPSFDYHLAEGVEKTFRRTYRENTVKALKLISDIEDNCTDKIK
                     EFEKRTGLYAKIDMHSEFLKRFNEFLIKECKADAKDIEKINKFAYNEALAETDKKTFQ
                     AMEGIVHNLNTMHSRAGAQVPFSSINFGTDISSEGRMITKNLLLAQDAGLGNGETPIF
                     PILIFKVKEGVNYNPEDPNYDLFKLACKVSARRLFPNFSFIDAPFNLKYYKPGNPDTE
                     VTYMGCRTRVLGNVCGEEVVSRRGNISFTSVNLPRIGLKHGVLLKGKFDEAGFFKELD
                     EKMDAVKQQLLERLVVQGSKKVKNFPFLMGQGLWMGSENLGWEDTLEDVVKQGTLTIG
                     FIGLAECLKALIGEHHGESEKAQELGLKIIKHMRDKCDEYMKQYTLNFSLIATPAEGL
                     SGRFTSLDKKVYGTIEGVTDKDYYTNSFHVPVYYKISAYDKLQKEAPYHTYTNAGHIS
                     YVELDGDPLKNLEAFEDIIRYMKECNIGYGSVNHPVDRDPVCGYTGIIEGAVCPKCNR
                     EEKDGPKFERIRRITGYLVGTLDNFNDFKAAEERDRVKHA"
     gene            23844..24362
                     /gene="nrdG"
                     /locus_tag="PLQ68_04845"
     CDS             23844..24362
                     /gene="nrdG"
                     /locus_tag="PLQ68_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004609444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0051353 - positive regulation of
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HXK72012.1"
                     /translation="MHKIRLSGKCIKDSTVDGPGLRIVVWTQGCLHNCEGCHNPNTHD
                     VFGGFLEDIDNVIKYISDSKIQKGITLSGGEPFLQPKELIPIARTALDKGMNVWAYSG
                     YTYEQIIADNEKKALLDVIDVLVDGPFVLSLKDSRLVYKGSKNQRIIDVKRSLKENKV
                     ILSQYDEINAEI"
     gene            24444..25142
                     /locus_tag="PLQ68_04850"
     CDS             24444..25142
                     /locus_tag="PLQ68_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HXK72013.1"
                     /translation="MYNILICDDDYDIVSALKIYLSCDDYKTYTAYNGRRAIEVIKEN
                     KIHLVLLDIMMPDVDGITAMVEIRKISNVPIILLTAKSEDNDKVIGLNLGADDYITKP
                     FNPVEVLARVKSQLRRYMQLGSETKHKSIVQIGNVELDDDEKTVNVNGEPISLTPIEF
                     QILKLLMQNPGKVYSTKQIYAEVWQDEPYGAENTVAVHIRHIREKIEINPAEPRYFKV
                     VWGQGYKMERQAEK"
     gene            25139..>27000
                     /locus_tag="PLQ68_04855"
     CDS             25139..>27000
                     /locus_tag="PLQ68_04855"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HXK72014.1"
                     /translation="MKNMSHGLLTKILAFFLIAVTAFTFGISVSASVFIKTYDIDRDP
                     EMAFFKSDLCSDITTDYAMEAYYGYFEQYNLTYPDQQTIEDYERYYSKEHTNFFFEIM
                     DESGKVVLSNYTDQYGYKQPFSMSKDKEGYTINAYVKKDITASDDYLFPYNLYNVLYK
                     IRNILIPIASLSLVLTIMLFVYLMYSAARKKNEEQFKPGFMDKIPFDILCVLIGGLIV
                     VAILTMEYNAPALYSEFMYQELIMLLSFGFAFIFLSLVLLFVSFAARIKLKTLFKNTL
                     VYLVFRFIFRCIKAVFKFIWKMLTKIPLLWKLILVLGAISLFELILIVLMARGTLIPV
                     GLWFMLRIAIVAMSCYAALNMIKIKKGGDAIARGNYSYRIDNRYMLSDFKQHAENLNN
                     ISSGMSYAVNEKMKSERFKSELITNVSHDIRTPLTSIINYVDLLDRQNIADEASKEYI
                     DILKRQSARLKKLTDDLIEASKATSGVITANLEKLNVKEILSQAVGEYTPRLLENQVE
                     PVLNEVDSNLYIKADGRLIWRVMDNMLGNICKYSQPGTRAYITVEQQSNRVRICFKNV
                     SKYKLNISSEELMERFVRGNSSRTGEGSGLGLSIAKSLTEIMKGAMLIEIEGDL"
BASE COUNT         9078 a         4436 c         5118 g         8368 t
ORIGIN      
        1 acaggatgtt atccttctaa acctatatat gaaggaacaa aagaagttga ctttgaccat
       61 acatgctggc ttattgatga tataactata gagcttattg cagattcaaa aggaatccat
      121 gtaagagaag acatgttaaa actcatatac aagataaagg gcagagacag gttagtactt
      181 gttactgatt caacgaattt cactaaggat aataacagca cagatgtgaa tatattaaat
      241 ggtacagatc ttgcaggttc agctttaaca ttgaacaaag catgttttaa tatgatgaag
      301 cacacggaca taagtataac tgatgcattc tatatggcat catatatgcc agcgagactg
      361 cttggtattg acgacataaa aggctcaata gagcctggta agaaagctga ccttattcta
      421 gtcgatgatt tgatgaaaat atatgaagtc tggcttgatg gccagatgat ttaactattc
      481 tttaccgttt acgaatgtta tttttgcatc tttgggaaga gcaagattac actcaacttc
      541 tgcagcttta acaatacctg caattaccgg acatcctcca tggcctggaa gattctcttt
      601 tgcatattca tataaagggc cgtctcctgg ttttgcaaga catagctcat atgcatcaaa
      661 tgtatttccg atttttttaa acatttcatt tatgcttacg catcctgatt tgactttcaa
      721 agtcacttcc atgccgtctt cagacacagc ttctactgat gttataagat tgcatattcc
      781 tggatctatt actacttttg tcatattgtg aatatccttt ctaacactaa aacacattgt
      841 acacctatta tgtgcttatg tgtatatgat ttatgtgatt tagtcactgg aaaatacttt
      901 atgggtgtta attctaacat ggatatgata gaattttatt gtaaagaagg gcggtgcgca
      961 tatgggcaga tttacagaac ggatatcact aagattgatt ccattgccaa aagaaatgtc
     1021 atgtaatgat ataaagaggg tggcattttc agatgtgaaa attgacgtaa tatttccaga
     1081 agattctacc ttattatctg caaaaagaaa actcataaca tatttcggta attcgatata
     1141 tggatcattt aggattcgaa tagtagaccc tatgcctaat acagtcaaag tattaaataa
     1201 ttttccaaat tcagaacagg cataccatat gtggtatgac ggtgcaataa atatttcagc
     1261 cataacctca aaaggtatta tgaatggcgt ccgtacattg cttcaggctg tatcaaaaga
     1321 agacaatgaa tttattattc ctcttttgtt catatatgac tatcctgata tccctcttcg
     1381 aggccagtgg ggaggagact ccatggatga tatagaattt acatcatctt tgaaactcaa
     1441 cagtttagat tacacaggca gtttcgatat taactcagaa aatatcggaa caggttatct
     1501 ggagaaaaag gaagtactgg aaaattgtat aaaagaagga attgttctta cggcttatgt
     1561 acctcatctt gaaaatgtta taggaagata tctcaataaa cctgagtata aaaaacttat
     1621 tgagaaaata aagaatgtgc cattacctga atcagcacag agaaacgatt acaagcctgg
     1681 cttgtgcgta tcctcagaac atactaaaaa acttgtatat gagtggatga gagaacttgc
     1741 tcagataaaa ggtgttaaat ctgtaatggt atggctgtca gaaaataccg aagcgtgtat
     1801 atgcgaaaag tgtaaagatc aggaaccttt cattttggaa accaagatgc ttgtggaatt
     1861 atttgaaaaa ttaaaaaaag agtttcctga tatcggatgc gagatacttt taacacaggg
     1921 cagttattca gttaatgaaa agatactaga aatagtccct gaatatatat cagtactgta
     1981 ctatcatgga agcaggacat acatctctga taaaaaccca atgatatatg aaggcctgat
     2041 gccatatatt gaaaggggcg ggaaattggg agtttatcct cagattactc atggttggag
     2101 atgcgtattc ccatggacag gaccggattt cattaaatac agatgtgatg aatttgttga
     2161 gaagaatatc cataaggtag taggatatgc agtaccatct aatgtttatc acagatttaa
     2221 tattgcagcc atggctgaat ggacatggaa taacaaagga agagacgaag aaagcttcat
     2281 gaaagcctat gcggctatta atggtattga cgctgatggt tttgtaaagt ggtgtaactt
     2341 aaatatggga cctgcatggg cattagcaga gaatagattt ttagtaggcc agatgtttaa
     2401 cttcccggta ataatgaaga gtaagtttga tatggaggat tttcgtttca ccgattatac
     2461 taaaaagcct ataaaagata tagataaata tataaggttg gcagacaggg cagtaaaggc
     2521 agcaaagcag ataggctata cacaaggaga actggaaagc agatgtatag tagatgtttt
     2581 gcgttgtgca ggagcttatc agaaattctt tacactgctg aataataaaa aaagaactca
     2641 tgaagaagat catcaaatga gtttaatgta tgagatgatg cacaataatt ctaagaatat
     2701 atatcacgca ttactggatt ggaaagaact aatagacaaa gataattccc atttccaaac
     2761 aagaattacc gatacagctg taatctgtat gaggattaca gatttcatga agaatatttt
     2821 gaataaactg gaaatttcac acaaagaaca tcattctttg gaatacagcg aacttggcag
     2881 atgggatgaa tcagtatttg ataaagcaag cctgacaacc attccatatg agatatcaaa
     2941 atttacagat aaagatggaa gatatcatat atgctttgat tttgtggaca gtaaaatagg
     3001 tactctgata aaagacatat cagttatgca gaatggaaag tgtatatcaa aaatagtata
     3061 tgatgcaaaa gttcctttta tgaaaagaat caatatatat gaaccatggt atgaggcgct
     3121 tgtggatgta tctggtatag aaaaaacatc tgacagaaca ttagaagtta ctctggaaaa
     3181 gccagacgag caaaaagcga cctgcagagg aatactgggt ataaggaaga tttaattatc
     3241 tgtactgagc gattgtttca acgagcgctt ccatattttc aaaaggaaaa ccgttgtcgg
     3301 cttctacgta aaaccaggag ccgccttctt gcatcctgaa tacttcatat tcctttttaa
     3361 cgagatcttt tacctgcgaa ggtgtaccaa atgtcattat attagccctg tctgtatgta
     3421 tagcgacagc aagttttagt gatctgcagt attttgcaaa ctccaccata tcgtatgcag
     3481 gaagctgtgg ccatatggaa tgaacaccta attcttttaa atcttcaagt atgggaagaa
     3541 cataaccgca actgtgaaaa tgtatatttt ttcctgcttt ttttataggt tcaaaaagcc
     3601 tttcccatct tggtttgaaa aagcttctcc ataaagaagg gcttaacagc atgctttttt
     3661 ctgttccgta atcatcacca aagtaaaaac catcaacatc aattgctaaa agatactgaa
     3721 tcttcttttc ataatactca actatcatat cagcaagctg gtttatttcc ttcgttcctt
     3781 ctgccatatc acagtagaca ttttcatcag gacacaaagc tttcagtttt tcaattattg
     3841 tccctgcaaa acgtgaaaga taatagtttt tcttatgttc aactgcctgc tgtttgtcta
     3901 ttaaaaactg ctcacttcca atatctgggt gggaggggac tatataatct tttagtttat
     3961 ccatatctga aaggggatag atatgaggta ttcctgcaat tccatatatc ctgtattccc
     4021 aaacagtatc ccattcatct tttttaaatt cgtgataatt accgttttca tcaagctgat
     4081 tttttgttaa aacaggtact tgcttgtatt caaaaggccc gaaatcatct ggatatgcct
     4141 tatatagctc attaagttta tctccatgtt caaaatagcc tacaggggtg tagttgtatt
     4201 gcagcgggac tttatctact ggtttgaaat gtattgcgga atttactctt tctctactgt
     4261 tcatatgaca cctcacaatg ttatgtgttg attataccaa ggaatcatag cagattaccc
     4321 atattatatg gattagttta attaatttca tttactagca tatattcagg atatatatcc
     4381 ctttttcttc aatgtttcat aaacttcttc tatttttctg gttactgtta cagcagcatc
     4441 agtttccttg atgcctatgc aggtatactc ataccccttc tcatttataa ctggaataat
     4501 ttcataatcc agcgggtttg gtccgaatag ttccttaagc ttttcagaaa attcaacact
     4561 gcatttaaat cttacgtgaa ggcatatatc ttctaaagat tttgatgtta ataaaaactc
     4621 aaaggtgaaa ggaacaccgc ttgttgaaag aaggtctagt gaacagctta catcagataa
     4681 tttaatatcg ttttctgcct taatgggctg ccatatcatg aagtctccga cagtattagt
     4741 aagatttgca tccacagcat caataatggc tctgtttctt gttcttatct tctgttctat
     4801 ttccatgcct gtattaactt ttgtatcttc cataatggaa gtaacagaag agaagaaaag
     4861 gatttctttg agatcattta gttcctgatc tgataatagc atgctcttgt ctatatcaac
     4921 tactgaaaca aaagctttca tgaaagagca cattctaaga agttcatctt cgtcaatttt
     4981 ttcctcttca tcaactaaac gcagttcctc aggaagacct ttgtaatttc tgactatttc
     5041 aggatctgca agactgcgta atgttttgcc tgtcagttcg ttattctttg aacttgtaac
     5101 aatcaaagaa tacagatgat cataagtcat ctgcatgcat attgtagcaa atagaagctt
     5161 ttgacgtaat tcttcctgta cttttgatgc cttgcagcag gaaattaaat ttaaaagctg
     5221 ataagtatta aaaagccttt ttatacttct tggattaaat cctatagagg ttttaataag
     5281 gtctttgtat tcactaataa tcttagaatt aactttcatt tccatcttca ccagcatgtc
     5341 gtatatatac tttttaatgt catactgagc gacaggcatc ttaaatggaa gctgtattat
     5401 cttgtcaaag aaactctttc ctttgtcatc atctaccaga ttgccgaatt tctgtttaac
     5461 gcctgcagta acaacagagt aatctactgc aagtataaat acacagttct cacaatctag
     5521 aaacacctta agtacttcta aaagttcaac tgccttagca ggatttaacc tgtccagatc
     5581 atctacaaaa atgacaactc tgtctttttt gctttctttt aatttatcat tgacactgtc
     5641 ttgaaaagcg tctttcaagt cgctgactgc ttttgcaata tctgcatctt ctttgttgaa
     5701 tacatcaacg attgctccac cgtccaaccc gactgtactt atagcggtct tggctccgat
     5761 ttttaaaatg cgaagactgg tttccaatat tttcgttgtt ctgttttcag cctttgaatg
     5821 aagttttgtg atgagatttt ctaaaaaaga cacgcataag tcttttccca tattgaactg
     5881 ggaatactgc caggtattga accagatagg atataccttg tcatctattt cttttttaat
     5941 catgctcatc atactggtct tgccgctgcc ccagtctccc tgaattgata ttgtcatggg
     6001 agtatcacag cttacaataa actggcataa accattaata tatgtcttta taccaaaaga
     6061 atcttctgtt aacttctcta cgggtttatc cgtatatccc atcttgctca tttcatgccc
     6121 ccctttgaaa tagctgattg atcttttacc acaaaatatg gattagttta caagcagatt
     6181 atacagaaaa acaggaataa aaacaattta aaacttcaat aagtgaatta ttctggatat
     6241 atgtaactga tgaaatatga atgtttgtgc taaagtcaca gataaccaag tgggtttgat
     6301 gtatagtaac agtagaaaca agcagtaaac aaggaggtca gccatgaaac atatagaaat
     6361 cggcaagaaa gcaccggaat tcatattaga agatatacat gagaaccaag taaagctttc
     6421 agattacaaa ggcaagaaag tacttctttc gtggcatccg cttgcatgga ccggagtatg
     6481 tacagatcag atgagagcct tggaagctaa tcatgaaaga tttgagaaac taaatactgt
     6541 cgcattagga ttaagtgtag atccagttcc tggaaaactg gcgtgggcat cagtgctgag
     6601 aattaaaaac acccctctgt taagtgattt ttggccacac ggcaaagcag caaaggatta
     6661 cggaatattc cttgaagata tgggtatatc cgaaagagcg aatgtaatta tagatgaaga
     6721 aggtatagtg agatggatga aaatatatcc cattgcacaa ctacctgaca tagaagaagt
     6781 atttaaaaag ctagaagaaa tttagtatga aataattgat caaaaaagta agcagttgct
     6841 gtaattgata gcaactgctt ttctttactt ccagtttcgc tggatgattt cactttttaa
     6901 gaatagtttg ctcttttgtg tggtttttag ggggtgtaac tttttgcgtc ggattaaatc
     6961 atctatattc tgtctggaac agttgagaag ttcagcggct tctgttgtat tgatgactcg
     7021 gttttcaaca aacaagcgga agtcctccaa agatagtgga atatctttac cgcaatcata
     7081 tagtatataa tctgcaatat tcagattttc accccagcag actccataac ctccagactg
     7141 catactgact ctgtaaaaca ttttctcatc ttttaagata ggtaaaaaag ctttattatc
     7201 tatcagtaaa gttttcatat tgcacttttt aacattacca tcacgaaaga agattaatag
     7261 atgcccattt gtgagtggaa caacatcctc tatcttcttt tgaaaacgtt taaagaaact
     7321 ttcgggaaaa tccttttcag aaacagatac cagataatag tcatcttgcg cacaacgtcc
     7381 atttgtaagc attaataaat caaactcatc ataagaatca agcttgttat ctttcaatat
     7441 ctgcccaata ttctgtctgt cagatgggat aatgcgttgc tgtacccata tcctgctcca
     7501 ataagcattt actgtcgtct caccacgctt aagaaaagag gatagtggaa gcggcgtttc
     7561 ccaagggtct gcgttttctg gcagttcaat gtaaaatcgt ttatcacatt cgtagtagat
     7621 caaaaatgca atatctttat tagcagaatc attattatct ctgattgcaa aaatcttcat
     7681 aaaacatcca cctcctccaa agcatttccc atattttatc tatatatgct tgtggtatga
     7741 ctgttgaaag atttgcaaac agtatctcat aatcagattc tttgagagga ttaaattgag
     7801 gtatactatt tttaggaatc aaattcaggt tatctttagc agaactggac ccgacaaaac
     7861 attgtactgg tttatctgcc ataatatcga atacatgaac agcatcttca tttttgcagc
     7921 tacaaagtaa ggagaggcca tggtcaaaca atggtgcaag gcgtagtgtt ttttcctttg
     7981 agttacgcac cacctctata ttggctccat gacggtctct gttcagtatc agaaaatcta
     8041 caactatcat cttccatata tattcagacc aatcgtgccg taagcaaaaa tccagtggtt
     8101 tttcaccagt ttctcgttca gcttgataat atgtatctaa ggctatcttg ctttcatttc
     8161 tttttttgaa atcagaagaa gcacaaaggc aagtttcatg gagttgtcca tcaattaaga
     8221 tgtcagcatg tatcaattga taattaagat gttcgacatc aagtattgta agcagacgat
     8281 cagcaatcag ttcatttata cactcatgtc cagtaattcc tttatatgca tcataatcgg
     8341 aaagcttata gtagacttta tctccaccta gatcggaata tgctttcaga aaagatccta
     8401 ctgcacctga catgttttgg attttagtcc atgaaagata tgtaagatcc tgcttttcaa
     8461 gaatgacttt tgtctgcatt atttcccctc ctcatggtaa acattatatg tatctacttg
     8521 tcatatgtca agtagatgct ctgtttgttt atataatcat ttccttaaaa acttggcata
     8581 ataaatttat attgaaccac atcagcaatt gaataaccga gcattcctgc tttaatatcc
     8641 caagaattaa cagctaggta tccatttaat tcttgcaaat gaccgaatag aagtgtcaca
     8701 aatgaccgaa ttgagtgata tggttgagtc tgacacaaat aaaaactaat taaagaaaaa
     8761 tgtggattac cacaaaaatc tttagttagt gtttgacaaa acaattttta agagtataat
     8821 aatattaaag aaaagtgtgt attagcacaa taatctttag tgaggtagtc aagtgcatat
     8881 tcaaagagac tatttattag aaaaactaaa agcagggatg aaatcaccgt taataaaagt
     8941 aattattggt gttagaagat gcggcaaatc atatttacta tttaatatct tttatgacta
     9001 tcttaaaagc ataaatgtga gagaagatca tattataaaa atcagacttg atgagttaaa
     9061 aaatgctgca ttaagaaatc cagtaaaact atcttcctat atcaatgaaa gaattctaaa
     9121 tgatggagag aagtattata ttcttcttga tgagattcaa gaatgtgtaa aaattgcaaa
     9181 tcctgatttc ctagacaatg aaggaaattt gattgaagga ataagtgtag atgaatcata
     9241 tttaacatat catgatgttt taaatgaatt attaaacatt gaatatgttg attgctatgt
     9301 aacaggaaat aactcctgct tactttcaag tgatttacct actgaattca gaaacagaaa
     9361 ttacaatatt gaagtttttc ctttgtcctt taaagaatta catcaagcat ttccagatat
     9421 gcagtatgat gaattgtgga atgagtatat aacatatggc ggaatgccac ttgcagtatt
     9481 gaatagaagt gttcaatcaa aacaaaatta tttaaaggat ttatttgaag gcacttattt
     9541 taaagatatt aaagaaagat atggtttgag ggatgaagca agtattgatg atgttactga
     9601 tatcgttgca tcatgtattg gttcaccctt gaatccagga aaaatagaaa atacatttaa
     9661 gtcaagaaaa tcaagtaata ttacagataa tacaatttcc aattatctaa aatacttaga
     9721 aaattcattt ttaattaaag aagctaaaag atatgatgtg aaaggaagaa aatatatagg
     9781 aactttaagt aaatactatt tcacagatat tggtcttaga aacgcaagat tgaattttag
     9841 gcaaatagaa gaatctcata taatagaaag cataatatat attgaacttg taagaaatgg
     9901 gtattcagtt gatgttggac aggttgaatc atggatacaa gatcctgata ctaaaaagag
     9961 atgtagagtt aattatgaag ttgattttat tgtaaatgaa ggaaatagaa agtactatat
    10021 tcaatcagct gttgaattat ccacaattga aaaggttaag aaagagcaaa agtcactaat
    10081 gaaaatagat gataatttta gaaagataat aattgtaaag ggatttagaa acccactata
    10141 taatgatgat ggaattttgg ttatcggttt gtacaatttc ttaatggatg taaaaataat
    10201 cgaacagtaa atacttaatt acgcattaaa agccgcttct tcatttattt gcttaaaaaa
    10261 gaccagagtt cagataatta tgtactgata cttaataatt aattgatgga agctagaagc
    10321 ttatactacc aaagtgaagc cgctatctgt ttcatactgg gtattgcatg tattaaaaaa
    10381 tccttttgcg ggcagtatcc ttcttcctgg aaatagaagt atctgtatct tttgcatccg
    10441 ccattgcaaa agctttttac ttcacaggtg tcacacattg aattttttgt agaattatct
    10501 gataggaagg ttaaaaagtt atctgattta aatatacttt caaaagaatc gttatgaata
    10561 ttacctcctc ggtactggtc cagtacataa aagtcacaag gataaactga accgtctgat
    10621 tctattacaa actgagcctg acagaatccc atcatgccgc actgttcagc tggatatcct
    10681 gcacacattt ttattaggtt atcaaacatc cttacactta tatactttcc ctgattatac
    10741 ttatcgagcc atatgtcata gaatgttttt aaaaattctg catatgcttt tggctctagg
    10801 gtatagagat cttctaagta gggcatattc aatggtgaga gacagaaaat cagctgaaca
    10861 taatcaaacc cattcttttc atacatattc cataaagcct gaggatggcg cgcattgtat
    10921 tttgtaataa ctgtaagtat gttatatggc acttcatgtt tttttaacag ccttatactg
    10981 ttcataatcc tgttaaaggt accttttttg ttattatcaa tcctaagctc atcatggatt
    11041 tcttttatcc catcaagtga aactcctact aaaaaatcat tacttttaaa gaatctgcac
    11101 cattgttcat caataagcag gccatttgtc tgtatagtat atgtgtactt tacttttggt
    11161 gttttaactt tttgtgcata tttaacaaag tcttcaaaga aatccagttt tgccagggta
    11221 ggttctccac cttgaaagga aaatccaatt tctccatttg tacagacttc cagtgcccgg
    11281 tcaataatct tataagaagt atcatatgac attatgccat atgactttat gtttctgtta
    11341 ttgcttacat cattatagaa gcaatattta caattcatat tgcacaaaga tgatgctggt
    11401 ttgattagta catgaaaatt atttatattc aataaccttt attaacctct tcatattgct
    11461 ttttgaaatc ttcaagtact gattttcgat acttctgagt aggtctttct gttataagta
    11521 cattaccgtc agtataaccc tcttcgcgtc cttcaagttc cttgataagg atatttctta
    11581 agtacttgat tctgtcggca tgctcagcgt catttatagc attatggctt tctgtcgggt
    11641 ctttatcaag tctgaaatac tgttcaatcc cgctttgtga aaaccatatg tatttatcat
    11701 actctgtgac tataaaatga ttggaataat atccgtaaga atgctcacca tgaatatatt
    11761 cactcttatt ggattcgttc ccgggaagaa agctctttcc gtcaatattg tctggtattt
    11821 ctatgttcgc aatatctaaa agagtgggca ttacatccct tagctctact aacttatcgg
    11881 aacgtttatg tgtatctttt atacattttt ctggtccgta aagtatcata ggtattctgg
    11941 cactcgactg ataaggaatg gcttttctgt acctcatatg atcacaaagc atttcaccat
    12001 ggtcagctgt gaataagatt attgtgtttt tcataagaca atcctgatgc attgcttcta
    12061 agagctttcc tatttcatag tctatatgag ttatacaggc gtaataacct gctcttgcca
    12121 tacgaatctg ttctttgtct atagggcctg actggctgtt gtatacacga ccttcttttt
    12181 ctaaaagatc aaaatcatcc cagtctccgg taataggttc agaaaggttt ttgtttgaat
    12241 acatatccag aaaagcttta ggcgcatcga aaggtggatg aggacgaaca tatgagatat
    12301 ttaagaagaa aggttttgac ctgtctcttc ttctgaaaaa atcaatagcc tgatcggtta
    12361 cccagtttgt cggatgtaaa tattcctcat acggccatgg tcttgtcatc cagcaattgc
    12421 tttcaagtcc tgtatcagta atatcgcgtg aaatacccat tttatcttta aaccagtaaa
    12481 aatagtcatc tgcaaatctg ctgtcttcat atgaaggcat tgaagtattt ctgtaactgt
    12541 ggacatatcc gtcatgaagt attacattat gaaatccgat taaatttctt attggataaa
    12601 catgcatctt gcctacagcc tgtgtatagt atcctgcatc tgataataca gaagccattg
    12661 tctgaggata attccactca actctgtcct ggtaacctac acgaccatga ttctttggag
    12721 aaagtccggt atgcaaagaa gctcttgatg cgatacagct tggacaagcc gtataggcat
    12781 tgcgataatg aataccctgt gatgccaaag tgtcaagata aggagtcttt acatcaggat
    12841 gtccgtcaaa ccctaaacag tcccctctga actggtcgca catgattaag attatatttg
    12901 gtctttccat gaaaattcac ctggtttctt ttttttaatt ttatcattgt tgaaatattc
    12961 agtcaaacaa gcaaatattg ataagataat aatatgttat actatatacg caacttaatc
    13021 ctatatgaat ttcaatatct ggaggtatgg ttaaatgaaa aaaatcttat gtatcttact
    13081 gattgtatca atgatgctga tattatgcgc ttgtgataaa actcagtgtg agacaccttc
    13141 aggaatatat tctacaaaag acaatatgtc caatattcat atttacagtg atggcacttt
    13201 tactattttt catatcatat ccagcagaat aataactggg gaatataagg ctgaagaaga
    13261 tttgcttgtt ttaactgatt tggaaaatga tgtatacaaa tttaatattg aaaaagacag
    13321 actgatattt ctatcgacta ctgctaaata tacatatttt gaagaagata tgacgtatta
    13381 tgcaaagcag attgattatg agaacataca ggcagcagta tattacggtt tttccagtta
    13441 tgctcttttt actgataatg atgatgtagt taaagaactg tatgatggat actataatat
    13501 gaaaactcaa gaaacagatg aagaactgga cttcccttca gccttcttag tcgtagtagg
    13561 ggatgtatca ttctttatgg ataaaaacgg atgtattagg cttaatgata atgattatat
    13621 agtaaaagac acagctaata gcataagtta tgcgcgactt aaggaaattt ataacgacaa
    13681 aagccttatg atgggtgaag actgaaaagt atctgtatat attgtataat agtaatagaa
    13741 taaaaaagcg gaggtaaata tgaaaaaaat agcatttttc gcattcagag gaaacgagat
    13801 gtgttttatc catattcttc ttaatgcact ggaaatgaaa caaaagggta aggatgttaa
    13861 gataatattt gaaggagaag ctgtaaaact gccaaagatt ttgtctgaca aggaaaatcc
    13921 gatgtataag aagtgtattg agtcaggcat tattgacggg gtctgtgaag catgttctaa
    13981 agctttagga gcatacgaag acaataaggc tttaggctta aattttctct cagaactaaa
    14041 aggacatcct tccatggaca gatatatgga agaggggtat gaaataatac tgatgtaaca
    14101 gcagcttgag gattaatttt aagtatgtgc tgtacatttg attgttggaa catgtaagag
    14161 ctgattattt ttatgaaaaa ctgattaata aaaaacctgt gacatacatc acagggcttt
    14221 ttatttttgg agctggtgga cggaatcgaa cccccgacct gctgattaca aatcagctgc
    14281 tctaccagct gagctacacc agcgttcttg gtgggaacaa tagggctcga acctatgacc
    14341 ctctgcttgt aaggcagatg ctctcccagc tgagctatgc tcccaagagt gtgtaccggt
    14401 gtaacagcat tttgaagtat actaaaaggt aaatgacttg tcaaccataa tatcaatatt
    14461 tttcaatatg ctaatttttt gaaatgctaa ttatgtcttg tataaaaatt tacttagttt
    14521 tatttttaag atttatttgt atttgcttgc aagtccgcct tcagaggttt ctctgtattt
    14581 gctgaaaaga tcttttcctg tctcatacat tgtatcaaca accatatcaa aagatatttt
    14641 tctggtatgg gttagaaaat ttgcaaggct tacagaattg atggctctca ttgcagcaac
    14701 ggcgtttctt tctatacagg gaatctgcac taaacccatt attggatcac atgttagtcc
    14761 taagtgatgt tccattgcta cttcgctagc atattctatc tggtcaagat ccatcccgaa
    14821 aagctctgca agccctgctg cagccataga acaagcactg cctatttcag cctggcatcc
    14881 gcattctgct cctgaaatgg aagcattggt tttaattaca tttcctatta cgccagctgt
    14941 tgctaatgct tttataatat ctttatcaga aaagccgttt ctgtcctgca tatacttcaa
    15001 aactgctggt agaactccgc aggctccgca tgtaggcgcg gtaacgacaa ttccacctcc
    15061 tgcgttttgt tctgatacag caaaagcata tgaacagaca atacggtttt cttttgtaga
    15121 agtactttca tccatatggc gctgactgaa caggtatttg gcttttctct gaagttgaag
    15181 tcctccttca agaattccag ttgcatttaa tccttctttt attgaaagct tcatctgctg
    15241 ccatacagtt tcgaggtaat cgcagatatc ctcaccttca gttttgatta cataatcgca
    15301 tattctcatg tcattttgtt cgcagtattg cttaatctga gtgaagtttt cctctttgta
    15361 aacattggat gtatccatta aaggttttcc ttcgattcgt atagctccac cgccaatact
    15421 cattacgcgc catttatcta ttactatctg gtctttaatg gccatgaaat ccattgtatt
    15481 aggatgagga atatcttctg aaaactggtc gacagtaata tcaacgtttt taggagagaa
    15541 agcttctctt attgctttat cggtcatatg gcccagggcg gttttagcta aagaaccata
    15601 cagagttgcc gtaaactcat cagcatctgg atgctcttct ttgaaccttc ttgctgctct
    15661 gtccggtccc attgtgtggg agcttgaagg ccctcttcct attctatata attccttcaa
    15721 agatttcatg tttctacctt cttttcggtt gccattatat catatatatt tattaatgct
    15781 acacctataa aaatatatat attaggagtc ttatctgtcg gtattaaaga gtgaatctga
    15841 ggagtgattt ttctgtaaat aaagaaaaaa tccgagggga gcggatttct tcttgctaaa
    15901 gatttgattg ctgacacatt agagtcaggg tacggaaagt attatacaat atacgaacat
    15961 atgtttcaat agttattttt ttactgtcca ataaatagtc agactggtta tgtgttgtat
    16021 tgattatgag ggatgatata taattgggag gtgcgaaagg ggtttttatg aagaataaaa
    16081 cagctatcaa aacaattaac tggcttgtcc ctattttcat tgtggggcta ataatattta
    16141 attcgacaac cgaagcactt gttcttgcag ttgcttattt catatacaga gtaattattg
    16201 aactttccag gatatattca tatatcgcaa ttaagaacta cagtaaggga aatcttataa
    16261 aagcaaggaa atactataaa aaagcatatg aagccaaagt aaaggaatta ccgatagtag
    16321 catcatatgc ttacttgtta attttattaa aagaatatga aaaggcagaa gaggtcttga
    16381 acgaactgga acagatgaaa cctgaaggaa aatccatgac atcattaatg ttatgcagag
    16441 ctgttctggc atggaaaaca cagaagaatc tgatacttgc attgtccaag atagaagcga
    16501 tggatgaaac aatgcgtaac cagtggtact ataatgttta tgcaaaactt tgcatattct
    16561 ctgaagatgt agaaaaagca aaacaagctg ctataaaagg atatgaatac ttaaagacta
    16621 accctgttgc aattgagaat cttcttataa tccactgtat aaacgaagat tatgataagg
    16681 ctttatcagc agcccagaag ctaataaatg gcaagaagaa tatgaagcct tcatcaaggg
    16741 atgcatatta ttactgcgca ttagcatatg agaagaataa cgatacagac atggcagcca
    16801 aattgtatgt taaggcattg gaatacgaag acacatcaat gacttatgct gacaaccagc
    16861 aagtaattag aaaagcaggg gaatatatga aatgaaaacg agtatgaata aaatctggcc
    16921 gaaggttttt gattacttca taattaccat aggagtttta ttatctgcag cagcggtcaa
    16981 tttatttttc ataccgtata agatacactc aggcggagta tctggtattg caacagtcct
    17041 gtattacata ttcaaagcaa agataccagt aggcgttctt atagttgcac tcaaccttcc
    17101 tttgtttcta gtagggctta aggttattgg caagactttt atggcaagaa cgctttacgc
    17161 tactttagca tatacagtcg ctattgattt aactgcacca tggctaacag ccctttcaaa
    17221 tgaaataact atagagagca ataatatacc tgatttgatg ctgttctgcc tgttcggagg
    17281 agctataatg gggtttggac tgggtatggt atttaccaga aattcctgta ccggagggtc
    17341 agaccttttt gcagagatac ttagaaaaaa aggaatacat ttaagtatgg gaacattgat
    17401 gttcatgttt gatgttatct ccatagtaat gtcgttaata gtattcaaaa atctggtact
    17461 tgtactttac tctatattag ctatttttat atcaatgaag gtaatagact tgatactgga
    17521 tggcgctaat gtcgccaaag cagtattcat catgtcggac aagtctgaac agattgcaga
    17581 tgcgattata aaggttcttg gaagaggcgt gaccggcttg agcggaagag gaatgtattt
    17641 caagcgggaa cgggaaacac tgttttgtgt agtgaaaaat aggcagcttc ctgcattgaa
    17701 aaaactagtc aagcagatgg atgaaaaagc ctttcttata attacatctg cacatcaggt
    17761 tcttggagaa ggttttggag catttgataa ggagctttag gctttgacaa taaaagatga
    17821 gatgatataa ttatttaggt taagcatata tttaataaat gctgaagcat atatatacat
    17881 tgacgtaaaa ggagtcatta atggaacaaa aactattatt atcattgaaa aggaaagctg
    17941 ctgaagtgag aaagaatata atcgaagagg ttttttctgc aaacagcgga catccaggcg
    18001 gatcgctttc ctgtacagac attcttactg ttttatattt caagcatatg aatgtagacc
    18061 caaagaagca gaaagatcct gacagagaca gatttgtatt atctaaagga catgcttctc
    18121 cagtactcta ttccatactc gcagaaaaag gctttatacc taaagaagac cttgttactt
    18181 tcaggcaggc tgacagttat cttgaaggtc atccttcagt aaaagatgtt ccaggcgttg
    18241 acatgaccac aggttctttg ggccaaggta tatcagcagc atgcggtatg gctcagatgg
    18301 gcaagcttga taagaaaaat tacagagttt atgctatctt aggtgatggt gaattacagg
    18361 aaggccaggt ttgggaagct atgatgtatg ctgctcacta taagctggat aaccttacag
    18421 cttttcttga ccataacggt cttcagatag atggcgatat aactaaagta atgtctccag
    18481 aacctgtagc tgcaaaattt gaatcctttg gatggaatgt aataatagtt gatggtcatg
    18541 atatgacgca aattgataat gctattgaaa aggctaaaga tacaaaggga aaacctacga
    18601 tgatagttgc cgaaaccatt aaaggaaaag gcgtgtcata catggaaaat caatatggtt
    18661 ggcatggcaa agctcctaat caggaattaa gagataaagc cattgcagaa cttgacgcat
    18721 atatcgagaa tttgggggta taatatgaaa aaagctacaa gagcagctta cggtgaagca
    18781 ttagcagaat acggaagcaa tgagaagata atagttttag atgctgattt atctaaatcc
    18841 acaatgacag catcatttaa agagttatat cctgaaagat tcttcaatac cggaatagcc
    18901 gaagcaaata tgatgtcatc agcagcaggg atggctacat gcggaaagat agtatttgca
    18961 agttcatttg caatatttgc agctataaga gcaggagagc agataagaaa ttccatatgt
    19021 tatccgaaac ttaacgttaa agtagctgca actcattcag gaatatcagt tggagaagat
    19081 ggagcaaccc atcaggccat agaggatata gctcttatga gagctttgcc taatatgaca
    19141 gttatacagc catgtgatgg aagagaaact aaacaggcag ttaaagccat aatagattat
    19201 gacggacctg tatatttaag attaggaaga cttgcagtag aagatatcta tgataattcc
    19261 tatgttttcc aaatgggaaa aggagtacag cttaaagacg gctgcgacct tactattatt
    19321 gctacaggtc ttatggtaaa acctgcactt gatgcatgtg aaattcttaa aactcagggt
    19381 attttagcca gggtaattaa tatacataca atcaaaccta ttgataagaa tattttaatt
    19441 aaagctgcaa gagaaacagg cgctatagta actgcagaag aacataatat tatcggtggt
    19501 ctcggttcag cggtatgtga ggtacttggt gaggaatggc ctgttcccat taaaagagtc
    19561 ggcatagaag ataaattcgg aaaatcagga aaaccagaag aattattgaa aatgtacgga
    19621 cttacagctg aaaatattgt aataaagtct aagatagcaa tggcattaaa aaagaaatga
    19681 tttttgaaag ccatgcgcat ttactttcaa acaagtttga aaacgacaga gatgaagtta
    19741 taaaaagagc tttagagaac ggcgtatatg ccgttctcaa tgtttctgat gatttaaaaa
    19801 catcagtaga agctgttaag ttttcaaaac agtataacaa catattcagc tctgcaggaa
    19861 tacatcctca taacgggatg gattttaagg atgatgattt aatagtcatc aagcaacttg
    19921 ctttagattc aaagacactg gcgataggtg aaatcggatt ggactactat tacgattttt
    19981 gtgaacgcaa tgtgcaaaaa gaagtttttg ttaaacagct ggagctggca aaatcattag
    20041 atatgccagt aataattcat gacagggagg cacataagga taccatagat actttgaact
    20101 tgttcaaagg aaacctagaa ggtgtgatac actgttattc aggaagtgct gaaagtgcaa
    20161 agatattatt ggatatgggc tattacatct cctttacagg cgtaataacc tttaaaaatg
    20221 caaacaaaac aattgaagct ttaaaagcag ttcctataga cagattactt ttagaaacag
    20281 acagtcctta tcttactcct gagccgtata gagggaaaag aaacgaacct aagaatcttg
    20341 tatatatcgc aaagatgatg gcacaggtaa aacaggtttc atatgaattt cttttggaaa
    20401 aggtgtggga gaatactttt gaattattta aaaaagctaa accgtataga aaggtgctgg
    20461 aagaaaaatg aaaaatgtag ataatataat acctcttccc aattatgtcc gtgcaaagaa
    20521 ggctctaggc cagaatttcc ttatagacaa aaccatagcc ttgcagatta cagatgcgct
    20581 ggagatgaat gaagatgata cggttataga agtaggttcg ggtacaggag cattaagttc
    20641 gcttatagcc ccacattgta aaaagctata tgctatagaa acagaccagg accttattaa
    20701 gccgctatca gcaaccttga gtgattacag taatgttgaa gttgtgaata aagatatact
    20761 gaagattgat ataaatacgc ttattaatac aaaagacggt tataagacag tagcaaacct
    20821 gccctattat attactactc cggttataaa caaatttatt gagcaggagc atcttcctga
    20881 tgtaatggta tttatgatgc agaaagaagt tgctgacagg atattggaaa aagaaggaag
    20941 caggatatac ggaagcttta ctatttatgt aaattacaga tatgcggctt cttttgtagc
    21001 ccatgttcct ccagaaagct tcataccctc accgaaagta accagcactg ttcttaaatt
    21061 cataaaaaga gataaaccat tagttgatgt accttcagaa gaatttttct tttctgttgt
    21121 aagagcgtct ttctcgcaaa gaagaaagaa gctttccaac tgtatttctt ctatgtcatc
    21181 attcaacata agtaaagagg atgttgaaaa gatattatct gatatggggc ttagggcaga
    21241 tatcagagca gaatcattat ccattcaaaa gttcgcagaa cttgcatgga agcttcactc
    21301 catctaaaca aaaaatgtaa gtcagaagca gttcttacat atgagtattt acacaaaata
    21361 tgtaaacgaa tatgcttaat ttaaacaagc taaatttttt aaattttatt tccaaactct
    21421 cttgcaaaaa gcataaggtg ctcatatact agatactacg tattgtgttt acaacctaat
    21481 acaaaacact atatatgggg agagtgccat gattatgaaa attactaaac gcgatgggcg
    21541 tgaagaaaac ttttcgcctc ttagaatcac aagagcgata ttcctagcag ctgaagcagt
    21601 agctaaggaa gagaacacaa aagcagattt tacaatagca gaagagctca cagacaaggt
    21661 tatacagctt cttaacagaa gatttattga aaccataccg acagtcgaag atgttcagga
    21721 tgctgttatt aaagtgctta ttgaagaagg tcatgcaagg acctctgaaa aatatattat
    21781 atacagagaa gcaagaaata aagcacggtc catgaagacg cgtcttatga gggcgataga
    21841 ggaaatcacc tttcatgatg ccaatcaaag taacttaaaa agagaaaatg caaacataga
    21901 cggaaatacc gcaatgggta ccatgcttca atacggttcc actgtatcaa aagaattctg
    21961 caaaaccaat cttttagatc cgcttcacgc caagatgcat gaagaagggg atatacatat
    22021 tcatgatatg gatttttaca acatgggaac tttgacatgc tgtcagattg atttaacacg
    22081 tttattcaag aaagggtttt caaccggtca tggacatctt cgcgaacctc aggatatagg
    22141 ctcatatgcg gctctggcag ctatagctat acagagcaat cagaatgacc agcatggcgg
    22201 acagagcata ccttcttttg attatcatct tgctgaaggt gtagagaaaa cattcagaag
    22261 gacttacagg gaaaatactg tcaaagcatt aaaactaata tctgacattg aggataactg
    22321 tacagataaa ataaaggaat ttgaaaaaag aacaggactt tatgcaaaga ttgatatgca
    22381 tagtgagttt ttaaagagat tcaacgaatt tttaataaaa gaatgcaaag cagatgctaa
    22441 ggatatagaa aagataaaca agttcgcata taacgaggca ttagctgaaa cagacaagaa
    22501 aacgttccag gcaatggaag gaattgttca taatctgaac accatgcatt caagagcagg
    22561 agcacaggtt cctttttcca gtatcaactt cggtacggat atctcctctg aaggcagaat
    22621 gattacaaag aatcttttat tagctcagga tgcaggactt ggtaatggtg agactcccat
    22681 attcccaata ctcatattca aagttaaaga aggagtcaac tataacccag aagatcctaa
    22741 ttatgatctt ttcaaacttg cgtgcaaagt ttcagcaaga aggctgtttc caaacttctc
    22801 atttatagat gcaccattca atcttaagta ctataaacca ggtaatcctg atacagaagt
    22861 cacctatatg ggatgcagaa caagagtact gggtaatgtg tgtggggaag aagtagtttc
    22921 cagaagaggc aatatatcat ttacatcggt taatcttcca agaatcggct tgaaacacgg
    22981 tgttctgcta aagggcaagt ttgatgaagc gggattcttt aaagaattag acgagaagat
    23041 ggatgccgta aaacagcagc ttcttgaaag actggttgtt cagggaagca agaaagttaa
    23101 gaatttccca ttccttatgg gccagggttt atggatgggt tcagaaaatc ttgggtggga
    23161 agatactctt gaggatgtcg taaagcaagg aacacttaca ataggattta taggactggc
    23221 tgaatgttta aaagccctta taggggagca tcatggagaa agcgaaaaag cacaggaatt
    23281 aggattgaag attataaaac atatgagaga taagtgcgat gaatatatga agcagtatac
    23341 tcttaatttc tctctaattg ctacacctgc agaagggctt tcaggaagat ttacatcact
    23401 tgacaagaaa gtttacggca ctatagaagg cgttactgat aaggattact atacaaactc
    23461 tttccatgta cctgtatatt acaaaatatc agcatatgat aagcttcaga aagaagctcc
    23521 atatcatact tatacaaacg caggacacat atcatatgtt gagctggatg gtgacccttt
    23581 aaagaattta gaagcatttg aagatattat aagatatatg aaagaatgta acataggata
    23641 tggaagcgtc aatcatccgg tggacagaga cccggtatgt ggatataccg gtattattga
    23701 aggtgcagta tgtcccaaat gcaacagaga agagaaagac ggacctaagt ttgaaagaat
    23761 acgcaggata acaggatatc ttgtgggaac gctggataac tttaatgatt tcaaggcagc
    23821 tgaggagaga gacagggtca agcatgcata agataaggct gtctggcaag tgtattaaag
    23881 acagtaccgt tgacggacca ggtttacgca tagtagtttg gactcaggga tgcttacata
    23941 actgtgaagg ttgtcacaat ccaaatactc atgatgtttt tggcggtttt ttagaagata
    24001 tagataacgt tataaaatat atatctgatt caaaaataca aaagggtatc actctgtcag
    24061 ggggtgaacc ttttttgcag ccaaaagagc ttataccaat tgcaagaacc gcattagata
    24121 aaggcatgaa tgtctgggca tattcaggtt atacttatga acagattata gctgataatg
    24181 agaagaaagc tcttcttgat gtcattgatg ttttagttga tggcccgttt gtattatctt
    24241 taaaggattc gagattagta tacaaaggat caaaaaatca gaggataatt gatgtgaagc
    24301 gctcattaaa ggaaaacaag gtaatattga gccaatatga cgaaataaat gccgaaattt
    24361 agaatcttaa tgaaatttta ataaaaaaag gtctttttcc ttaagtattt atagtgcaca
    24421 atagaaatag aaggagacgg acaatgtata acatattaat ttgtgatgat gactatgata
    24481 ttgtttcagc actgaaaatc tatttgtcgt gtgatgatta caagacatat actgcctata
    24541 acggaagaag agctatagag gtaataaagg aaaacaagat tcatcttgtg cttttggata
    24601 taatgatgcc tgatgttgat ggtattaccg ctatggtgga aatcaggaaa atcagtaatg
    24661 tccctataat tcttctaaca gccaaaagtg aagataatga taaagtcatc ggacttaatc
    24721 ttggagcaga tgactatatc acaaaacctt ttaatcctgt tgaagtttta gcaagggtaa
    24781 aatcgcaact tagaagatac atgcagttag gttctgaaac taaacataaa tcaatagtgc
    24841 agatagggaa tgtcgaactt gatgatgatg aaaaaacagt caatgttaat ggagaaccta
    24901 tcagtctgac acctatagaa tttcaaattc tgaaacttct tatgcagaat ccggggaagg
    24961 tatattcaac aaaacagata tatgccgagg tgtggcagga cgaaccatat ggtgcagaga
    25021 acactgtagc tgttcatata aggcatataa gggaaaagat tgaaattaat cctgctgaac
    25081 cacgatactt caaagtagtt tggggacagg ggtacaagat ggaaaggcag gcggagaaat
    25141 gaaaaatatg agccacggcc ttttaactaa aatattagca ttctttctaa tagcagttac
    25201 tgcatttaca ttcggtattt cagtttcagc ttctgtattt attaaaacat atgatataga
    25261 cagagatcct gaaatggcat tctttaagag cgacttgtgt tcggatatta caacagacta
    25321 tgccatggaa gcatattatg ggtatttcga acaatataac ctgacatatc ctgaccagca
    25381 gacaatagaa gattacgagc gttattactc aaaagagcat accaatttct tctttgaaat
    25441 aatggatgaa tcaggaaaag tagtactttc aaattacaca gatcaatacg gatacaaaca
    25501 gcctttttct atgtctaaag ataaagaagg atacactata aatgcatatg taaaaaaaga
    25561 tattacagct tcagatgatt atctttttcc atataacctt tataatgtat tgtacaagat
    25621 cagaaacatc cttataccca tagcttcact gtcactggtt cttaccataa tgctgtttgt
    25681 ttatctgatg tattcagctg caagaaagaa gaatgaagag cagtttaaac caggctttat
    25741 ggataaaatt ccatttgata tactgtgcgt attaataggc gggcttattg tagttgcaat
    25801 acttactatg gagtataatg ctcctgcttt atattcagaa ttcatgtatc aggagcttat
    25861 aatgcttcta tccttcggat tcgcatttat ctttttatca cttgtccttt tatttgtgtc
    25921 ttttgctgca aggattaaac tgaaaaccct ttttaaaaac actttggtgt atcttgtttt
    25981 tcgattcatt ttcagatgca ttaaggcagt tttcaaattt atatggaaga tgcttaccaa
    26041 gatacctctt ttatggaagc ttattcttgt tctaggtgca atatctcttt ttgaacttat
    26101 cttaatagta ttaatggcaa gaggaacttt aatacctgta ggtttatggt ttatgctaag
    26161 aatcgctata gtggcaatgt cttgttatgc tgctcttaat atgataaaga ttaaaaaagg
    26221 cggagatgcc atagcaagag gtaattacag ttacaggatt gataacagat atatgttaag
    26281 cgatttcaaa cagcatgcag aaaaccttaa caatatatct tcgggaatgt cctatgcagt
    26341 taacgagaaa atgaaatctg agagattcaa gagtgaactc attaccaatg tttctcatga
    26401 tatacgtaca ccgcttacct caataattaa ttatgttgat cttcttgaca gacagaatat
    26461 agctgatgag gcgtccaagg aatatattga tatattaaaa agacagagtg caagacttaa
    26521 aaaacttaca gacgatctga ttgaagcatc aaaagcgaca tccggagtta taactgcgaa
    26581 tttagaaaag cttaatgtta aagagatact tagtcaggca gtaggtgaat ataccccgag
    26641 gcttttggaa aatcaggtag aacctgtttt aaatgaagtg gacagcaatc tttatatcaa
    26701 agcagatgga agactcatat ggagggtaat ggataacatg ctgggaaata tttgtaagta
    26761 ttcacagcct ggtacaagag catatattac tgttgagcaa cagtcaaaca gggtgcggat
    26821 atgctttaag aatgtttcaa aatacaaact taacatatct tctgaagaac ttatggagcg
    26881 ttttgttaga ggcaactcat caagaacagg agagggtagc ggattggggt tatcaattgc
    26941 aaagagtctt actgaaatta tgaaaggcgc gatgcttata gagatagaag gggatctttt
//
LOCUS       DAOSXM010000023        26567 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2111
            NODE_1162_length_26567_cov_11.473371, whole genome shotgun
            sequence.
ACCESSION   DAOSXM010000023 DAOSXM010000000
VERSION     DAOSXM010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864178
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 26567)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26567)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:24:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,261
            CDSs (total)                      :: 2,229
            Genes (coding)                    :: 2,218
            CDSs (with protein)               :: 2,218
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 3 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26567
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1162_length_26567_cov_11.473371"
                     /isolate="AMR_MDS_2111"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            151..690
                     /locus_tag="PLQ68_04860"
     CDS             151..690
                     /locus_tag="PLQ68_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007061612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HXK72015.1"
                     /translation="MNSFYELASKRQSCRNYSDRPVEKDKLMQCINTALIAPSACNGQ
                     PWHYTLVTNRDTASKLAKTLQSGGMNRFADKTPAFAVITEKSSNASATIGGKLKHQDY
                     SSMDIGISAANFVLQAEDLGLSTCIIGWFNEKDLKSLLAIKDADRIRLVIAVGYKDEA
                     DKFRTKKRKPQDEVLKVIE"
     gene            complement(796..1323)
                     /locus_tag="PLQ68_04865"
     CDS             complement(796..1323)
                     /locus_tag="PLQ68_04865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72016.1"
                     /translation="MKNCKIQGSGRVTEGEYDIITIEGAGKLVDDVTVNTVNVSGVMI
                     AKGKLRAKEIKSIGMIKLFKEADIDSIQIDKGVLISKSDINSTLLECRGAIRVKGGIN
                     SDIVKIEGKGKVDYIVGDNIIIANNSQRENKERLDKFKVNRIEGTSIEMHNVNCMNME
                     GDFIKMTGKSVVGRI"
     gene            complement(1320..1823)
                     /locus_tag="PLQ68_04870"
     CDS             complement(1320..1823)
                     /locus_tag="PLQ68_04870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72017.1"
                     /translation="MISNIKSTTFESYHNFTIGPDCNFKNIINVNKILSGDKIYCNNL
                     ISFSSIVTDLINADNIYITSVAGTKVKQIEGEYVWIGRQLPRHERITNIPKTLKNLII
                     CKIRKIKKVEVDTIEADVIDIDYVKATKISGEIVNVGNNCIVDVVEYSKDLNLSKKAI
                     VKSVVKL"
     gene            complement(1987..2328)
                     /locus_tag="PLQ68_04875"
     CDS             complement(1987..2328)
                     /locus_tag="PLQ68_04875"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HXK72018.1"
                     /translation="MNINEEYLVRLMINQLERKGEIRTVLKGESMRPAINSKDKVTIR
                     KVDIKSIVVGDIIAYKRNDARNITIHRVVNICDNYRIILKTKGDNNNNEDPYIVDESS
                     FIGIVLRDPII"
     gene            complement(2352..4139)
                     /locus_tag="PLQ68_04880"
     CDS             complement(2352..4139)
                     /locus_tag="PLQ68_04880"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXK72019.1"
                     /translation="MKKTILFSVLKLIIKEKPIISISIIIAVSITAIFAPLYLILTKE
                     LFDYASKAISDLNNELSVLSLIFLLIKFFGVSFGEYLIMKINKGLLDMLDIEMKNILQ
                     NKLMNKVNELNYDKFDESDFYDSIKIAMDEIQSGSIVKSFSSILYIFMNIITLIAVSI
                     ILLKFSILAIIVSIICCLPGFLHQAWYSKENWDFIMSKVSITRKLDYLFFITTSKKTF
                     KENKEYKSFNYFIHKYDALYCQYLTDIIKFNKKNLFLGSSYSLIHAIGTIIIICWIYL
                     ISAKGEVTIGEAVMYVAATQKLYNIIQNLIHWFGDASNNIKKANGVISFLKIKAEGRI
                     YSSMQHINHIESIEFINVTFRYPNTEKYVIKNMSFNIKKGEKIAIVGENGAGKSTLFK
                     LLVGLYHTNEGEILINGINVNNINNECIYKNIAVCFQELNAYSFSIRENIGFGNISSI
                     NNDIEILEAAEKSGFDSVIEKGEISINHFINRDFSNSGIVLSEGEKQRLALARAFMHK
                     GSVIILDEPSACLDIKTENEIFEKTFNLVKEKTVIFITHRLSNIIFADKIIFIKNGEI
                     KGKGRHVELLNSSIEYSELYNMQAGSFID"
     gene            complement(4117..5982)
                     /locus_tag="PLQ68_04885"
     CDS             complement(4117..5982)
                     /locus_tag="PLQ68_04885"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXK72020.1"
                     /translation="MKKNKCDFIPKIKIYIKEIYKPIEMIWQEDKMIVLLLFVNSLVR
                     KIYIPIIAFITKRIIEIIEIANSIGFSLELQNKIIILFVLLFIPFALNRLWWPVNTYT
                     QAIATTKLTNSIKKKIMVKICDTSLCFFDEEECYNIYENCIKESNGKRILEILQNLLN
                     FIATIIMFILLAFPMFIFNPILSILLILSAIPGVIIEKIFAKKIFLHETNNTLLSRMI
                     KYIFELFSNKQSVKEIKVFRIYDYLEKKHSEKYKEYNQGIKKLMQKSLIISLPAFFIL
                     QIMTLIGYYFTVTKVTNGSINIGEMSFIITAIGSLTVSFITISDAFNRTFTSNLYVQN
                     LYKFYNHGYIEEMQGEDVVHMENNEISFKNVWFKYPNAKEYTLKNICLTIKESEFIAL
                     VGLNGAGKTTIIKLLLRLYKPTEGEILYNGIDISSFSIDKWREIFSVCFQDFFDYSLS
                     LKEAIGIGNVNKIDLLANIKQAAEFAGLANVIDGLPMGYDTPLSREFDNEGVELSKGQ
                     KSKIAIARAFMSDGQILLFDEPTASLDARAENDYFESLKKLIRNKTCLIITHRLTSIK
                     SVDRILVLSNGEIVEDGNHEALMRNKGFYFQLYETQSEKYMKLAKEGKNEKNYTI"
     gene            complement(6463..7683)
                     /locus_tag="PLQ68_04890"
                     /pseudo
     CDS             complement(6463..7683)
                     /locus_tag="PLQ68_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612034.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(7784..9151)
                     /locus_tag="PLQ68_04895"
     CDS             complement(7784..9151)
                     /locus_tag="PLQ68_04895"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HXK72021.1"
                     /translation="MVKQIRENGNTDLEYIKLSKDIFLIAQYNCAIKICKYGKHIYNL
                     ENMLLNILLYIKATKRTYPSSFLLNCPELSQKVEFLLTNDCNLDCIYCYSCSDRDKKT
                     MDFNEARKVIDLVIKSAQIKKSYFKKEGKISISFHGGGEPTIAFNIIKKITEYANCEC
                     NKKNIEVEYDITTNGIFSREVIMWLINNNYTIHFSMDGLKINQDMQRPTISGKSSFDI
                     VAKNIDIAQNNSAKYTIRQTITKNNVGNIVKSIRYIIKRWKGVSSIKLAPLQQTNKSQ
                     ENSIQGPNFTRYFSKIRKAIRILENNHMNTDGITGGVSSLINGGSCAATRFEQLIITP
                     YGKITTCHEEVNSNQFIYGDVHNRVSINYKRVKELKNDDKKEIEEGYCSSCAYRMVCL
                     GGCKHRRFSESRDFFCMLQREMYKLCILQLFRKGNLNGYFKIEKIRINEMNIELINFR
                     QNRVK"
     gene            complement(9145..10428)
                     /locus_tag="PLQ68_04900"
     CDS             complement(9145..10428)
                     /locus_tag="PLQ68_04900"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HXK72022.1"
                     /translation="MRIKITENLYYYPRYRTITEENSDYANPRTIEVVKDLSKTEQSS
                     VEVLNPNFSKAAEILLTDECNLECKYCYSGEYRSKQKMSKEYVREIVKHLFKNAKYSK
                     LMGSSQETVYILFHGGGEPTLAWDELVEAITYSTELSRIHKIKLKLRLATNGMISKER
                     IKYLLDMNVEFRVSLDGIRKVNDQTRVGKNNQSVFNVVADNIDIINSHNNKFFIASVV
                     TGDNYNQMTMMTKYCIERWPNIHDIRFFMLEDTLLVRSNGVKPIAMVRYEIEKNKCIE
                     ILLDCKIGKYKEYRNITDYYIKNFSYPCMPSLLHMPVFNCDNNILRCNAHVFNNDALI
                     GVYNGKIQMSEEKYIELHNRLPRINLGCENCISQNNCEFALKYCVDPISHPVDYCSYK
                     KAFFKGILINKYEEAKMMKEYLEINNFGIKGIINW"
     gene            complement(10487..10771)
                     /locus_tag="PLQ68_04905"
     CDS             complement(10487..10771)
                     /locus_tag="PLQ68_04905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72023.1"
                     /translation="MKNKKFIKNTELDRADFENTIILTSKGEDEIMHVLNSSASLIFD
                     LANNKSCEEIFDSYISKFSDINGEDISVVREDYEKALNELIVKKILLEND"
     gene            complement(10995..11969)
                     /locus_tag="PLQ68_04910"
     CDS             complement(10995..11969)
                     /locus_tag="PLQ68_04910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72024.1"
                     /translation="MKYEFIAKNKVNGMFRFLMKDTDSNMLNQVFIYEKDNEEILQNY
                     IYDFLIRNNNQIDYVEKKYSIMVKTPFSNIAFSFYNIPKSFIDFFYFIYAPVIVFNVN
                     CDYEVELLFQDNNFFEIKSEYMSSCDLIYRVMNVIHICLCRIISKDATANKCFVLHAS
                     AVKTVNNNIIMIVGDKLSGKTSTLLNYIQNKGIYIGDEALVYDGEKILSYYMPLKVDN
                     NSVLDFTLKDELIESPSYIKGKNFLTHIFWQDFGLVKPTHILFLKRDKFCSIDALSGY
                     EAINRIIANTRNPVVINSFIKTGQNICNISERYLVSEVNNVNITNFIE"
     gene            complement(12052..12855)
                     /locus_tag="PLQ68_04915"
     CDS             complement(12052..12855)
                     /locus_tag="PLQ68_04915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72025.1"
                     /translation="MIYNCFGKYRIEINDPELLKNAGLIYGKSITNEKDDTKLIVIEK
                     VGNHIYYFLNEHLIYQANCQNWILKSVQLIEAVLKDQEPDKFIMIHGSCVQMYGRIFI
                     FTGNSGGGKSTLIYNLITNYNAYYVCDDIVLYDKFSKRLVTIPNKPMHLRYDTNNGDC
                     YCVSSDINSPMYIVPVKSKQTFNKKNCELKDIVLVDLEYKKNTITEIKELSAYGSILK
                     LIHRTMNISDLKKSDLIKFFSKISHYYTCIYSGEIIDVIKFMSMHEIAN"
     gene            complement(13120..14682)
                     /locus_tag="PLQ68_04920"
     CDS             complement(13120..14682)
                     /locus_tag="PLQ68_04920"
                     /inference="COORDINATES: protein motif:HMM:NF026257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HXK72026.1"
                     /translation="MKSCIIKEEVICKVIELSKPIVGKKYTLNDAEITEIMKLSTFQG
                     VKGAVLKNIMCNSRNDKYSNEFWLDKTVYLLKEKRIKKLLKIFNDSGITYTVIKGLSY
                     NYNVYSSSELRHFGDVDILVSSKDTKRAHATLIKSDYNVVDDCLDCKSIKERFRYQSH
                     LNLYKGDKLFIELHQAKNSYINLEEIVKNRQFVNDIYITDLIDTFIIACTHAWHHFSY
                     SISSLKFKSQFRLKLLLDIYRSYLKINQMNIRDIELLERADTVKAKCTVLNAVVMAEE
                     IYGDFFINRRYLEFSKFRYPDKYFDYNISLTDRLFNGDEVYKKVYSLYKGVNNQILIQ
                     SGANFSKDMLMQFEEKTLNSFFLSTLNATSTPIHEKFDYKAKFLWDSCFLYFLLNIES
                     QYCTKLTKIERYIGSYNHFEVSISYDGIINVFFLQAKKNNSIYFYRDNVIYRMKKSFT
                     EVVNRNCILYNIAIPWINIGIIPKKSMKIKLEITYIEKYYQKGIMNMYSLFAGECSNV
                     YENDFWITAILK"
     gene            complement(14908..16176)
                     /locus_tag="PLQ68_04925"
     CDS             complement(14908..16176)
                     /locus_tag="PLQ68_04925"
                     /inference="COORDINATES: protein motif:HMM:TIGR01426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72027.1"
                     /translation="MGKCVYFSYMHYGHIIPNLEMLKELSLRGEKLICYSSSTYSHLF
                     KGFDCEFREYDNLNEIYNMFCMGYPVNAFVQDSLLQRVLATSLFFVQTLDREKAITKY
                     YSDLLREENIDYMIYDFASFWGKSIAIELGVKSICCFEIPVFPTNIYNVDIKHFLSYF
                     DFRYLLRDQIFLKPDRIDDDVASELKIILDAYFTLMRIKGYDDFVCMHKSDFLNIVHI
                     PKELQAYSEELDSTYKFIGYKTPNIDNTNTEKEKCIYISQGTVFTQKHIPFVEKSINA
                     FRESPYKVVISLGMDNRIKDIEDYFSELPDNITIGKNINQIDVLSNCLLYITSGGISG
                     IKEALSLGVPVLLVGMPDQTYGMLEKLNLGRIADKWTITEDELFNLSVDIMNDKIIKS
                     CCMRMSEVFLNTDGYVEGVNCILNEVSCKG"
     gene            complement(16169..16918)
                     /locus_tag="PLQ68_04930"
     CDS             complement(16169..16918)
                     /locus_tag="PLQ68_04930"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HXK72028.1"
                     /translation="MISIILCAGKGSRISNLNCPNKCLLRFQDVSLLQKTLSYVNELD
                     EISKVIIVVNYNKDEVIAEAKKSTHKELVIVEQRNLNGIIGAIEQCIPVIGDDDFMLM
                     LGDEYYEMPKYKDFISCFINSELSILAGIVYVDDKTRISKTYGVTVKNDKLIYFEEKP
                     KNSINNIMGTGTILFRNSTLKYMNKIEVNEQRNERDLVDLLNASLNDNEQVHIYEISK
                     HYYNINDNHEIEDLSAFLEYRKKGKEESKNG"
     gene            complement(17026..17577)
                     /gene="loaP"
                     /locus_tag="PLQ68_04935"
     CDS             complement(17026..17577)
                     /gene="loaP"
                     /locus_tag="PLQ68_04935"
                     /inference="COORDINATES: protein motif:HMM:NF033641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator LoaP"
                     /protein_id="HXK72029.1"
                     /translation="MPTHQDEKAYYYILHVITGKEEQVCTKIQNCLCNQSFTPFTFKK
                     NVSFKRGKEISEVTRIIFPGYVFIKSCIAEAAFLIIIMDFVRKTNDIFKLLNYGSYEK
                     IAVNETEINFLLDLCNDNYCIEYSKGLIEGDRVKVIEGPLKGQESRILKINKQKKNAV
                     ITVFLVNEVRTITIGLDYITKAN"
     gene            17922..18239
                     /locus_tag="PLQ68_04940"
     CDS             17922..18239
                     /locus_tag="PLQ68_04940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72030.1"
                     /translation="MANYTTDTYAMKREILTFTNNLTKGLHIPKRKFITDMSYGMLAS
                     NSYLLSDIADTLHEEAKKKNTIERLSLNFAKEIPAQLAANYLAKAKTSQAAIQRYTSM
                     TEI"
     gene            18479..18607
                     /locus_tag="PLQ68_04945"
                     /pseudo
     CDS             18479..18607
                     /locus_tag="PLQ68_04945"
                     /inference="COORDINATES: protein motif:HMM:NF013828.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            18743..19133
                     /locus_tag="PLQ68_04950"
                     /pseudo
     CDS             18743..19133
                     /locus_tag="PLQ68_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612034.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(19579..19839)
                     /locus_tag="PLQ68_04955"
     CDS             complement(19579..19839)
                     /locus_tag="PLQ68_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020816846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03905 family TSCPD domain-containing
                     protein"
                     /protein_id="HXK72031.1"
                     /translation="MYKYKTKGTCSSEITFDIQDGKLNNVKFKGGCSGNLQGIGVLVE
                     GMETEKAISLLKGIKCGHKKTSCPDQLSIAINEVLEEEKNGK"
     gene            complement(19848..20429)
                     /gene="purN"
                     /locus_tag="PLQ68_04960"
     CDS             complement(19848..20429)
                     /gene="purN"
                     /locus_tag="PLQ68_04960"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018465572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HXK72032.1"
                     /translation="MMNICVFASGGGSNMQAVIDACKNKKIDAKVVLVISNNSKAYAL
                     ERAENEKIDNYHVSEYKYKEEFAGKLLNLLARYKIDLVLLAGYMKKLPKELVERYKVL
                     NIHPALLPKFGGQGMYGERVHKAVLEAKEKESGCTVHLVDEYYDNGKILNQIKVPVLE
                     DDTAESLAKRVLEKEHIIYVDTIRKILTKEIEL"
     gene            complement(20452..22989)
                     /locus_tag="PLQ68_04965"
     CDS             complement(20452..22989)
                     /locus_tag="PLQ68_04965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72033.1"
                     /translation="MKKGILSFITIILLLSICINALAYCETYELTGEHMNASYISDEH
                     TSRGHAYGYICQDCGLITILGYKYISTCCQCTGNHNYCLFSIGPHTAQGHLFTLRCLD
                     CGHTTSYYDIDYSCCQCVGHTPGPIEISQEHLYGLGHYYTITCKECGEIIESGYTTLW
                     SCSICNCDHVYVTEISDYHTPGLGHFYTRYCYKCGEVIDSGYTTIPHCCECRGYHNYY
                     YSVSDTHTEYGHLVTQRCVDCGHEEYYYTTVPTCCYCRGYHNYTYTVSNEHAPNGHLK
                     TGMCSDCGHVTTSYVTLPECCQCRGYHNFSQWSEVSSVHEAQGHRQTRTCKDCGYTET
                     RFTTYRPCCLCHGHDMQRTSNIGEHTDFGHAVVYRCITCGYEETVYEDIFGCPLCSNT
                     PTNQEEFILWIQHLGIPVKSINGNYTVSFTMYRDYGYIVYGNPYNVANNPCVAGEYQF
                     LGYTYDEDLFTNISYQNTEMGPVNANQWIYTDIPGATESWDRLESTVQKPYMLHTNLI
                     GHGAVNFTANDIGISKTRVQNGASWLSTGSIYTYKTNGYYATFKVPSMGTPDVSLNTC
                     FTQERLVYSDTDVYKKGYVNVSFSVDKPLEEIEYIKLSVQGGNSITLQPHERSGQLEI
                     NIPIQYIMPAKYTVRVLIEVKSIFNDVYITGDYCSVDLYKEEAPYNPANPVIPEEIIS
                     DEPEDIPYQEPDESQYDVCITDISLNGAWNIWGLNERFTGLENVQFNVSVAGRARDIT
                     VIFSEELKSHVYEDGYGNTYDYADLLGSYDMCPKDMHIEGLWNNGYTLTKSYILPLCL
                     DTIDTEGNRIKDKYTITVIAKNEKKEHIKEYMIDITGNIYDYLYVQP"
     gene            complement(23051..24235)
                     /locus_tag="PLQ68_04970"
     CDS             complement(23051..24235)
                     /locus_tag="PLQ68_04970"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HXK72034.1"
                     /translation="MKNIAKNRLVSLLLIISFILLSGLLEINVNASGSRFTDIESSQW
                     YFEPVIQLYGMNIIDGYPDNTFKPEGNVKTDELIKMIIKALGFDIQNSKDYWAQNYID
                     KAEEIGMLEGFGAEYTKDINRGQAAVLISNSMTYLKEPEYNTEEVYPFDLGMYTPVEY
                     IQHIKKCFSAGIINGFEDFTFRYKETLTRAQACTLVIKVMDKSKRDTSVRMDCSFLSN
                     KEQENIRYVGVGFDELSDSIKHTLLQVKDDIGYTVVEKRVYETKNTYEIRYHKSSLYS
                     NPSYCMFALRFFEGETGYPEKQWGYDTMFLKLELKSLDWEDGIPVSYYKSKVKNAICA
                     ALSEYDQYDIADYIMKKYDFIRSLDTHDAYNLLEDNSNDELRYVFFTMENVIGNFTFS
                     SK"
     gene            complement(24314..25543)
                     /locus_tag="PLQ68_04975"
     CDS             complement(24314..25543)
                     /locus_tag="PLQ68_04975"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXK72035.1"
                     /translation="MYFHRHIEPVVERIAKRKSVIVLTGARQVGKSTMLKNVCGNINF
                     VSLSDLLVRESAKENPSLFFDLHKPPVIVDEIQKAGELFEYIKDIVDEKKIKGQFYLT
                     GSQSMKLMKNVTDSLAGRTGVIKMLGLSMRELKGSSWRKPFLPILEDMMNDEKGSVEY
                     DYLKFVSYIHKGFFPELYETESDLKDWSDFYSSYFQTYIEKDIKDVLNIQDESAFIKF
                     VKAVAARTGEVLNMSAISEICGKDDKTVRSWLSVLESSGLVYLLEPYYNNYNKRLVKS
                     PKLYFLDTGLACWLLGWNTPEQMVNGAMWGHIFESYVFAEILKSYYNDGIVKPLIYYY
                     RDMDKNEIDLLVEEGDYLYPIEIKTSSNPTKSMVKAFHCLEKNPVKKIGTGALICFTK
                     ELLPLTDSVWMLPVHTI"
     gene            complement(25792..>26567)
                     /locus_tag="PLQ68_04980"
     CDS             complement(25792..>26567)
                     /locus_tag="PLQ68_04980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72036.1"
                     /translation="DIKENEILNDNVYYLNGIDLERKYIYVNELYILPNDYPLNLIEL
                     YFARESGERMLLALRNNEIFYLTDNIWGRYVGVRVKDFNDDGENEIAFINDPRATYGR
                     KRFVHIYNEKDFVAIPFSTNSVYDVIESILEYDVVYIEDEQACLATIKNKNTNDQIVI
                     KISSDTVKPNFIIDYREYLTSNIVNNGITENTVLFLINTENWMLVQVLDIEVIYEMQN
                     NKFVVKDIKYTFTFDFSKIYGLDEYKFEIIENSITDTNP"
BASE COUNT         8318 a         4928 c         3431 g         9890 t
ORIGIN      
        1 gtgaattcgg attagctaat gcagcttcaa ccgttgctaa ccttttaggt tatgaagcgc
       61 atgagaactg ggaagaaagc ataatagagt agtgcttaac aatatagtta atatataaaa
      121 ggttatagtt aattttcaag gagaataaca atgaacagtt tttatgaatt agcatcaaaa
      181 agacagagct gcagaaatta ctctgacaga ccagttgaga aagataagct gatgcagtgt
      241 ataaatacag cacttattgc tccttcagca tgtaatgggc agccctggca ttacacgctt
      301 gttaccaatc gcgacacagc atcaaaactt gccaagacct tacagtcagg cggaatgaac
      361 aggtttgcag ataagacgcc tgcctttgca gtaataactg aaaagtcatc aaatgcatca
      421 gcaaccattg ggggaaaact taaacatcag gattattctt ctatggacat aggcatatct
      481 gctgcgaatt tcgtactgca ggcagaggac ttaggattgt caacatgtat aatagggtgg
      541 tttaatgaaa aggatcttaa atcgcttctt gctatcaagg acgcagacag aataaggctg
      601 gttatagcgg taggttacaa ggacgaagca gacaagttcc gtacaaaaaa aagaaaacct
      661 caagacgagg tcttaaaggt aatagaatag cggctttttt attgtcctag atattattgg
      721 ataatgtctt tatctatcaa gttttactat atgcttaact ttactatctg gatccatttc
      781 caatgacttc gtgtatcata ttcgtccaac aactgattta ccggtcatct ttatgaaatc
      841 gccttccata ttcatgcaat tcacattatg catttcaatt gaggtacctt caattctgtt
      901 aaccttgaac ttatcaagtc tttctttatt ctctctttgt gagttgtttg ctattataat
      961 gttatctccg actatgtaat ccactttacc tttgccttca atcttcacta tatccgaatt
     1021 aataccaccc tttaccctaa tagctcccct gcactccaat aaagtagaat taatatcgct
     1081 tttactaata aggacacctt tatctatctg tatagaatca atatcagctt ccttgaaaag
     1141 cttaatcata ccaattgatt ttatttcttt tgctctaagt tttccttttg caatcattac
     1201 tccagacaca ttaacagtat ttactgtaac atcatcaacc agttttcctg caccttcaat
     1261 tgtaataata tcgtactcac cttctgtgac tctgcctgag ccctgtattt tacagttttt
     1321 catagtttaa ccacactctt cacaattgct tttttactta ggtttaagtc tttactatat
     1381 tcaacaacat ccacgatgca gttatttccg acattaacga tttctccgct tattttagtt
     1441 gctttgacat aatcaatatc aattacatcc gcttcaatag tatcaacttc aaccttttta
     1501 atttttctta ttttacatat tattagattt ttaagtgtct ttggtatgtt tgttattctt
     1561 tcatgtcgag gtaattgtct tcctatccat acatattcgc cctctatctg ttttactttt
     1621 gttccagcaa cagaagtaat ataaatgttg tcagcattta ttaagtcagt tactattgaa
     1681 gaaaaagata ttaagttatt acaataaatc ttatctcctg acaatatctt gttcacatta
     1741 attatattct taaaattaca gtctggtccg atcgtaaaat tatgataact ttcaaatgta
     1801 gttgatttta tattactaat caaacaactt gcttttacat ctgattgtat actatctaac
     1861 tctttttacc tgataagatg ctgtttgaca cttagcataa ataagtaatg tcttcataga
     1921 tcggagacta tagtgttact aagcgttgac tcaaacatca ccaaatgctt tttgtcagct
     1981 aataatttaa attattggat ctcttagaac aattcctatg aagctacttt cgtccacaat
     2041 atatggatct tcattattat tgttatctcc cttggttttt aaaataattc gataattatc
     2101 gcatatatta acaactctat gaatagttat atttctcgca tcatttcttt tgtaagctat
     2161 tatatctcca acaacaatac tttttatatc aacttttctt atagtcactt tatcttttga
     2221 attaatcgcc ggtctcatgc tttcaccttt taaaactgtt cttatttcac cttttctttc
     2281 caattgatta atcatcaatc ttaccaagta ttcttcatta atattcatta gatatttcgt
     2341 tttcattttc tttaatctat aaaactccct gcttgcatat tatataattc cgagtattca
     2401 atgctactat ttagcaattc tacatgtctt cctttacctt tgatttcacc atttttaata
     2461 aagattattt tgtctgcaaa aattatatta gataatctat gggtaataaa gataactgtt
     2521 ttttccttta ccaaattaaa ggttttctca aatatctcgt tttctgtctt aatatctaaa
     2581 caagcacttg gttcgtctag tattattacc gaacccttat gcataaaggc tcttgctaaa
     2641 gccaatcttt gtttttcgcc ttctgaaagt actataccag aattagaaaa atctctattt
     2701 ataaaatgat tgatgctaat ttctcctttt tctataactg aatcaaatcc actcttttct
     2761 gcagcttcca atatttcaat atcattatta attgaactaa tatttccgaa ccctatattt
     2821 tctctaatgc taaatgaata tgcattaagc tcttgaaaac aaactgcaat attcttgtaa
     2881 atacattcat tattaatatt gttaacatta attccgttta ttaaaatttc cccttcattt
     2941 gtatggtata aacccaccag aagcttaaat aaagttgatt ttcctgctcc attctcacct
     3001 acaattgcaa tcttttctcc ttttttgata ttaaaagaca tattttttat tacatatttt
     3061 tctgtattag ggtatctgaa agtaacattt ataaactcta tcgattcaat atgattaatg
     3121 tgttgcatac ttgaataaat tcttccttct gctttaattt ttaaaaaact aataactcca
     3181 ttagcttttt taatattatt tgatgcatct ccaaaccaat gaataaggtt ttgaataatg
     3241 ttatacaact tttgagttgc agcaacatac ataactgcct ctcctattgt tacttcccct
     3301 tttgctgaaa ttaagtatat ccaacaaatt atgataatag ttccaattgc atgaatgagt
     3361 gaatagctac tgcctaaaaa tagatttttt ttgttgaact ttattatgtc cgttaaatac
     3421 tgacaataaa gtgcatcata cttatgtata aaataattaa aagatttata ttctttattc
     3481 tccttgaagg ttttttttga ggttgtgata aagaataaat aatccaattt ccttgttatt
     3541 gatacttttg acataatgaa atcccaattt tctttagaat accaagcttg atgcaaaaat
     3601 cccggcaaac agcagattat actaacaatt attgccaata tcgagaattt taatagtata
     3661 attgaaacag ctattaatgt aataatattc ataaagatat ataagatgct tgagaaactt
     3721 ttaactatac ttccgctttg tatttcatcc attgctattt taatagaatc atagaaatca
     3781 ctttcatcaa atttatcata attcaattca tttactttat tcatcaattt attttgtaat
     3841 atgtttttca tttcaatgtc aagcatatct agtagacctt tattgatctt cataattaaa
     3901 tattcgccaa aagacactcc aaaaaacttt ataagtaaaa atattaaact caatactgag
     3961 agttcattat ttaagtcact aattgcctta cttgcataat caaaaagctc ttttgtcaga
     4021 attaagtata atggtgcaaa tattgctgta atactcactg ctattattat actgatggaa
     4081 attatcggtt tttcttttat tattagtttc agtacactaa atagtatagt ttttttcatt
     4141 tttaccttct ttggctaact tcatatattt ttctgactga gtttcataaa gttgaaaata
     4201 aaaacctttg tttctcatca acgcttcatg gtttccatct tctactattt ccccattact
     4261 taacacaagt atcctatcta ccgatttaat tgatgtcaat ctatgcgtaa taatcaaaca
     4321 ggttttatta cgtattaact tctttaaact ttcaaaataa tcattttctg ctcttgcatc
     4381 taaacttgct gttggttcat caaataataa aatttgccca tcactcataa atgctctagc
     4441 tatcgcaatt ttactttttt gcccttttga taattctaca ccttcattat caaattctct
     4501 agataaagga gtatcatatc ccattggtaa tccatcaatg acattggcta aacccgcaaa
     4561 ctctgctgct tgctttatgt tagccaataa atcaatttta ttcacatttc ctattcctat
     4621 agcttctttt aatgatagtg aatagtcaaa aaaatcttga aaacatacag aaaatatttc
     4681 tctccactta tcaatactaa atgaactaat gtctatccca ttgtagagaa tctcgccttc
     4741 tgttggttta tatagtctta aaaggagttt aataatagta gttttcccag ctccattcaa
     4801 tcctactaat gcaataaatt cagattcttt tattgtaagg catatatttt ttaaagtata
     4861 ctcttttgca tttgggtatt taaaccatac attcttaaaa cttatttcgt tgttttccat
     4921 atgcactaca tcttctcctt gcatttcttc gatatatcca tgattataaa acttatacag
     4981 attttgaaca tataaattgc ttgtaaatgt tctattaaat gcatcgctta tagttataaa
     5041 tgaaactgtt aatgaaccaa ttgcagtaat aataaaagac atttctccaa tgtttatact
     5101 accattagtt acttttgtaa cggtgaaata gtatccaatc agtgtcatta tctgcaatat
     5161 aaaaaaggct ggcaagctta ttattaagga tttctgcatt agtttcttaa ttccttgatt
     5221 atattcttta tatttctcac tatgcttttt ctctaagtag tcatatattc tgaaaacttt
     5281 aatctcttta acggattgtt tgttactaaa gagctcaaat atatatttaa tcattcgaga
     5341 taataaagtg ttattagttt catgtaaaaa tatcttttta gcaaatattt tttcaattat
     5401 aacccccgga attgcactaa gaattagtaa gatagataat atgggattaa atatgaacat
     5461 tggaaaagcc agtagtataa acattataat cgtagcaata aaatttaata agttttgcaa
     5521 tatctccaga attcgtttac catttgattc ttttatacaa ttctcataaa tattataaca
     5581 ttcttcttca tcaaagaagc ataaactagt atcgcaaatt tttaccataa ttttcttttt
     5641 tattgaattt gttaacttag tagttgcaat cgcttgtgta taagtgttaa ccggccacca
     5701 tagtctattc aatgcaaaag gtataaatag taatacaaat agaattatta ttttattttg
     5761 gagttctaag gaaaatccaa tcgagtttgc aatttcaata atctctatta ttctttttgt
     5821 tataaaagca attattggga tatatatttt tcgtactaga gagttaacga ataaaagcaa
     5881 aactatcatt ttgtcttcct gccaaatcat ctctataggt ttataaattt cttttatgta
     5941 gattttaatt tttggaataa aatcacactt attctttttc atttctcacc tttaactatt
     6001 gttgtattct ttagtgtgcc aaataagaag ttaatcatat ctagattcat gccattctat
     6061 atccggtcac tgttaccttc cttaaggatt aagtaaccta gttcgtgaag cagcttccat
     6121 tgatgccatt tactatctcc aactttttcc ttgctaatac aggaaagaac gaatccatta
     6181 tgaaacgtgt tggacaactc ataatcgatt atgtattaac catacaagtc aagaatggca
     6241 agtaggtaca taatcccttt tctcctcctg atatattata tatctatctt gtatgataca
     6301 atcagaccta taattagcag aagccataaa agatataact tacatgaagt gtctaataac
     6361 tcctgttcaa aatgggctcg agacattccg ttaaatctca ttttattcat tagctgcatt
     6421 aatgcttttc tttaatcagg atatgccttt tagatatgtc ggttatatta cagtaactgt
     6481 atgtaaagtt ggataggcta cacttgattg gacaactttt ttaagggtat cattcctgta
     6541 ctcgattggg ctcatgtatc ccaatgatga atgtatcctg ctgctgttga accagttaac
     6601 ataattcatg aactccagtt ctaatctttc caatgtcgca aaccagtttc ctttcacaaa
     6661 cacagtcttc actatcttgc atgttgcttc tgcaatcgca ttgtcatatt ggttgccctt
     6721 cctgcttaat gatctttcta tcccgaaagc ttaaaggatt ccctcaatga ttctgttttt
     6781 aactcattac ctctgtcagt atggaatata tgtatgtttg acaagtttcc cttaatcctc
     6841 gaaaatgctt tgaatacgag cttagaattc ttgttagctc ctgtgctgtc ctatgatctc
     6901 cctgttgaac aggtccacca gcacgcatat gtagttccat ctcatcccga ccctcacata
     6961 tgtaagatca cttactacgg cgttcctgta ggggcggttg ttgaagtccc tctgaagcat
     7021 attggccgtc ttttcctcat tgcacgtgct ctttgccggc ctgtattgtg ctaaagtatc
     7081 tgaataccac gccttcaagc ttcattatcc ggctcttgta ccttcttgaa gtcactatgc
     7141 cttctctact gagctcagcc tttatcttcc tcgtgccgta attgctcctg ctgctcatga
     7201 atatgtattc ataaatatat ctattactgc aggcttaagc ccgtcttcct tttccttctt
     7261 cctgtattcg taataatata cgttccttta tatcttaagg actctgcaca ttgctgatat
     7321 ccggtatttg tgggcattac gccttatcac acttaccttg tatgatacag tcagtggctg
     7381 tgcagacctt tcttcttgat tttttcagac actgcttgtt ttaaatatcg ttctccatct
     7441 cgagctgctt gtttcttttt ctgagctcta tcagctcgtt ctcttcatca gtcctgttgt
     7501 ccttctcggt gaatgaccct gtggattcac tctgatttat ccatttgtct agcaatagtg
     7561 cgcagagacc ataatctctt agaatatcgc actttctttt cccattctgg taaagatgca
     7621 ccatttgctc tggaactccg gagtgaatgt cttgcattcc tttctttgta ttgatctagt
     7681 cataattgat acatataatc caattaatat tttattatac cattttcaat accatttttt
     7741 ttctgtctaa tttagtgttg cttactaata atttcttgtt ctatcattta accctattct
     7801 gtctgaaatt aattaattca atgttcattt cattaattct gattttttca atcttaaagt
     7861 atccattaag atttcctttc ctaaatagtt gcagtataca taatttatac atttctcttt
     7921 gtaacatgca aaagaaatct cttgattcag aaaatcttct atgcttgcaa cctccaagac
     7981 ataccatcct atacgcgcaa gaagaacagt aaccttcttc aatttccttt ttgtcatcat
     8041 tcttaagttc ttttactctt ttataattaa tagaaactct attatgaaca tcaccataaa
     8101 taaattgatt tgaatttact tcttcatgac atgttgtaat tttcccatat ggtgtaataa
     8161 ttaattgctc aaatcttgtt gcggcgcacg atcctccatt aattagtgag ctaactccac
     8221 ctgttattcc atcagtattc atatgattat tttcaagaat tctaatagct tttcttattt
     8281 tactaaaata tcttgtgaaa tttggtcctt gaattgaatt ttcttgactt ttatttgttt
     8341 gttgtaaagg tgctagtttt attgacgaaa ctcctttcca tcgcttaata atatatctaa
     8401 tacttttaac tatgtttcct acattatttt tagtgattgt ttgccgtatt gtatactttg
     8461 cactattatt ttgagcaata tcgatatttt tagctacaat atcaaaagaa cttttcccac
     8521 taattgttgg tctctgcata tcttgattaa ttttcaaacc atccattgag aaatggatag
     8581 tatagttatt attaataagc cacataataa cttcccgact aaaaatccca tttgttgtaa
     8641 tatcatattc aacttctatg ttctttttat tacattcgca gttcgcatac tcagttatct
     8701 tttttattat attaaatgca attgttggct ctccacctcc atgaaaacta attgatattt
     8761 tcccttcctt tttgaaataa cttttcttaa tttgtgctga tttaataact aaatcaataa
     8821 cttttcgagc ttcattaaaa tccatagttt ttttatctct atcactacag ctgtaacaat
     8881 atatacaatc caagttacag tcatttgtta acaaaaattc aactttttgt gataattctg
     8941 ggcaatttaa taaaaagcta gaaggataag ttctttttgt agctttaata taaagaagaa
     9001 tattaagcag catattctca agattatata tgtgttttcc atatttacat attttaattg
     9061 cacaattata ttgtgcaatt aaaaaaatat cttttgataa cttgatatat tctaaatctg
     9121 tattaccatt ttcccttatt tgttttacca atttataata ccttttattc caaaattatt
     9181 aatctctaaa tactctttca tcattttagc ttcctcatat ttattaatta atattccctt
     9241 aaaaaatgct ttcttgtagc tgcaatagtc aacgggatga ctaataggat ctacacaata
     9301 cttaagtgca aactcacaat tattttgaga tatgcaattt tcgcaaccta aattgattct
     9361 tggcaatcta ttatgtaact cgatgtattt ttcttcgctc atttggattt taccgttata
     9421 aactcctatc agtgcatcat tattaaacac atgagcatta caccttaaaa tattattatc
     9481 gcagttgaac actggcatat gcagtagaga tggcatacat gggtaactaa aattcttaat
     9541 ataataatcg gtaatattcc ggtattcttt atacttcccg attttgcagt ctaaaaggat
     9601 ttctatacac ttgttttttt cgatttcata cctaaccatt gctataggct tgacgccatt
     9661 cgatctcact aataaagtat cttcaagcat aaagaaccga atatcatgaa tattgggcca
     9721 tctttctata cagtacttgg tcatcatagt catttgatta taattgtccc cagttacaac
     9781 actagcaata aaaaatttat tgttatgcga attaattata tcaatattat cagctactac
     9841 attaaaaacg ctttgattgt tttttcccac cctggtctga tcatttactt ttcggatacc
     9901 atcaagagac actctaaact ccacattcat atccaataag tactttattc tttcttttga
     9961 tatcattccg tttgttgcta atctcagttt tagtttgatc ttgtgaattc ttgataactc
    10021 agttgaatat gttattgctt caaccaactc atcccaggct agtgttggtt ctccaccacc
    10081 atgaaatagt atatagacag tttcttgact actccccatt aatttactat attttgcatt
    10141 tttaaaaaga tgctttacta tttctcttac atattcctta ctcatttttt gttttgaacg
    10201 atattcccca gaataacaat atttgcactc caaattgcat tcatctgtca gtaaaatttc
    10261 tgcagctttt gaaaagtttg gatttaaaac ttcaactgaa gattgctctg tttttgataa
    10321 atctttaact acttcaattg ttctaggatt tgcgtaatca ctattttctt ctgtaattgt
    10381 tctatatctg ggatagtaat ataaattttc tgtaatttta attctcataa caatattatg
    10441 caaaaaggtt gcttattcct ttccctaatt agtaaaatat tcattgctaa tcattttcta
    10501 atagtatttt tttaacaatt aactcattta atgctttctc ataatcctcc ctaactactg
    10561 aaatatcctc cccattaata tcagaaaatt tgctaatata actatcaaat atttcctcac
    10621 aggatttgtt atttgctaag tcaaatatca atgatgcgct gctgttaaga acatgcatga
    10681 tttcatcttc tccttttgat gtaagtatta tggtgttttc gaagtcagct ctatccaact
    10741 ccgtgttttt tataaatttt ttatttttca tagttagtct ccttgttaat catcagcttt
    10801 atataataaa caggcagatg aatatacagt taatctctgc tgcctccacc agcacagcat
    10861 acagatccgc atgctaactc aagcgcttca agttcttcct ttgagtaagt tgctatttca
    10921 ggcttttcat atttcatttt gtcacctcct ccctatatag aaatactagt aaatatttaa
    10981 aaagcatctg cctgttattc aatgaaatta gtaatattta cattgtttac ttcagaaact
    11041 aagtatcttt cagaaatatt acaaatattc tgcccagttt taataaaaga gttaattacg
    11101 acaggatttc gcgtatttgc aattattcta ttaattgctt catatccaga aagtgcatca
    11161 atactacaaa atttatctcg ttttaaaaac aatatatgtg ttggtttaac aagtccaaaa
    11221 tcttgccaga atatatgtgt taagaagttt ttccctttta tatagcttgg tgattcaatt
    11281 agttcatcct ttaaggtaaa atcaagaaca ctattattat ctaccttcaa aggcatatag
    11341 taagaaagaa tcttctctcc atcatataca agtgcttcat ctccaatata tattcctttg
    11401 ttctgaatat agtttaatag agttgatgtt tttcctgaca gtttatctcc gacaatcata
    11461 ataatattat tattaactgt cttaacagca cttgcgtgta gaacaaaaca tttattagca
    11521 gtagcatctt ttgatataat tcgacaaaga caaatgtgaa taacattcat tactctgtat
    11581 attaagtcac atgatgacat atattcagac ttaatctcaa agaaattgtt gtcttgaaaa
    11641 agtaattcga cttcataatc acaattaaca ttaaaaacaa taactggagc ataaataaaa
    11701 taaaagaaat cgataaacga ttttggtata ttataaaaag aaaatgcgat atttgaaaat
    11761 ggtgttttta ccataatact gtattttttt tctacataat ctatctgatt gttgttcctt
    11821 attagaaaat catatatgta attttgtaga atctcctcat tgtctttttc ataaataaaa
    11881 acttggttca acatatttga atcggtatcc ttcataagaa atctaaacat tccattcact
    11941 ttatttttag caataaattc atatttcatt ttttataatc cttataagac acttattccc
    12001 tttatataga tatttctatt ccctttatag ctttaactat ttggacttaa cttaatttgc
    12061 aatttcatgc atagacataa attttattac atcaattatt tctccactat aaatgcatgt
    12121 ataataatgt gatattttag agaaaaattt aattaaatca ctttttttaa gatctgatat
    12181 attcattgtt cgatgaatta atttcaaaat tgaaccatat gctgaaagct cttttatttc
    12241 tgttatcgtg ttttttttat attctaaatc aactaataca atgtctttta actcgcaatt
    12301 ttttttatta aacgtttgtt ttgatttaac agggacaata tacattggac tatttatatc
    12361 acttgaaaca caataacaat ctccattatt ggtatcatat cttaaatgca ttggtttgtt
    12421 aggtattgta actaatctct tactaaattt atcatataaa acaatatcat cacatacata
    12481 gtatgcatta tagtttgtaa ttagattgta tattaaagtt gatttaccgc caccactatt
    12541 ccctgtaaaa ataaaaattc tcccatacat ttgcacacat gaaccatgaa tcataataaa
    12601 tttatcaggt tcttgatctt ttaacacagc ctctattaat tgaacacttt tcaatatcca
    12661 attttggcaa ttagcttgat atattaggtg ctcgtttaga aaataataaa tatgattacc
    12721 tactttttca attactatta attttgtatc atctttttca tttgtaatac tttttccgta
    12781 aattaaacct gcatttttta agagttctgg gtcattaatt tcaattctat atttaccaaa
    12841 acaattgtat atcatattat tacaccttta ataattaact tcctttctca taataataca
    12901 taagtagatt tataatcaca tcagaacact agtagtatac ttctataaaa taacttaaca
    12961 aaattataac atcactgatt tttcagatca taatcactaa ttattatgtt atacacttat
    13021 ctcttccttt atctcatatg tttacacgat atatactcta ctaaaatgat gacttaacat
    13081 acctcaagct tcattttaaa taaaacaact gctaaattgc tatttcaata ttgctgttat
    13141 ccaaaaatca ttttcataca catttgaaca ttctcctgca aatagtgaat acatattcat
    13201 aattcccttt tgataatatt tctcaatata agtgatttcc aattttattt tcatactttt
    13261 tttaggaata attccaatat taatccaagg tattgcgata ttgtataaaa tgcagtttcg
    13321 attaacaact tctgtaaagg attttttcat tctataaata acattgtctc tataaaaata
    13381 tatggaatta ttttttttag cttgtagaaa gaaaacatta attattccat cataagagat
    13441 agaaacttca aaatgattat aactacctat gtatctttct atttttgtta gttttgtaca
    13501 atattgcgat tcaatattaa gtaagaagta taaaaaacaa gaatcccata aaaacttagc
    13561 tttataatca aatttttcat gtataggagt cgaagtagca tttaatgttg ataaaaagaa
    13621 agaatttagt gttttctctt caaattgcat taacatatcc ttactaaaat tagctccact
    13681 ttgaatcaaa atttgattat taaccccttt atataagcta taaacttttt tatatacttc
    13741 atctccattg aaaagtctat cagtaagtga aatattgtaa tcaaaatact tatctggata
    13801 tctaaattta gaaaactcaa ggtatcttct gttaataaaa aaatcaccat aaatttcctc
    13861 agccattaca actgcattaa gtactgtaca tttagcttta acagtatccg ctctttctaa
    13921 tagttctata tctcgaatat tcatttgatt aatttttaag taacttctgt aaatatcaag
    13981 taataatttc aatctgaact gacttttaaa ttttaatgaa ctgattgagt atgaaaaatg
    14041 atgccacgca tgcgtacaag caataataaa tgtgtcaatt aaatcagtaa tatatatatc
    14101 atttacaaat tgtctattct taacaatttc ttctaaatta atatagctgt tttttgcttg
    14161 atgaagctct ataaacaatt tgtcaccttt atacaaattt aagtgtgatt gatatctaaa
    14221 tctctcttta atgcttttac aatcaagaca atcatcaact acattataat cgctttttat
    14281 aagtgttgca tgtgctcttt ttgtatcttt tgaactaact aaaatatcga catcaccaaa
    14341 atgccgtagt tcgcttgatg aatagacatt atagttgtaa cttaatccct taatcacagt
    14401 ataagtaatc cctgaatcat taaatatttt aagtagcttt tttattcttt tttctttaag
    14461 taaataaaca gttttatcta gccaaaattc atttgaatac ttatcattcc tgctattaca
    14521 cattatattc tttagaactg cacccttcac accttgaaaa gtacttaatt tcataatttc
    14581 cgtaatttct gcatcattaa gagtatattt ctttccaaca ataggtttac tcaattctat
    14641 aactttacag attacttctt ccttaattat acagcttttc atgatttttg tacctctaac
    14701 ctttaatgat ttttctctaa tttcctgaag tcactaacta cactgctcct caatgaaaaa
    14761 tgttttattc attccacctc tcttgcctat taaccattac ttgtaaggct tctttgcgaa
    14821 attattaatt ataatatgag gtcaagactt agattttctt catacttttt tcgtaaacgt
    14881 taaatatgca tccacaaata taattcatta ccctttacaa gaaacctcat taagtatgca
    14941 atttactcct tcgacatatc catcagtatt caaaaaaact tcagacattc tcatacaaca
    15001 tgatttaata attttatcat tcatgatatc tacacttagg ttgaataatt catcttcagt
    15061 tatcgtccat ttatctgcta ttctaccgag attgagtttt tccaacatcc catatgtttg
    15121 atcaggcatt cctacaagta gaacaggtac tcccaaagat aaagcttctt ttattccact
    15181 tatacccccg cttgtaatgt aaagcaaaca attacttaaa acatcaattt ggttgatatt
    15241 tttgccaatg gttatattat caggaagctc tgaaaagtaa tcttcaatat cttttattct
    15301 attatccata ccaagggaaa tgacaacttt gtatggcgat tctctaaatg cattaatact
    15361 tttttctacg aaagggatat gtttctgtgt aaatactgta ccttgagaaa tatatatgca
    15421 tttttccttt tctgtatttg tattatctat attcggtgtt ttatatccga taaatttata
    15481 agttgaatcc aattcttcag aataggcttg cagttctttt ggtatatgta caatgtttag
    15541 aaaatcagat ttgtgcatac atacaaaatc atcatatcct tttattctca taagagtaaa
    15601 atatgcatca agaattattt taagttcaga agccacgtca tcatcaattc tgtctggttt
    15661 tagaaatatt tgatctctta gcaaatatct aaaatcgaaa tatgataaaa agtgtttaat
    15721 atcaacatta taaatattgg ttgggaaaac cggaatctca aagcaacaga tgctttttac
    15781 acctaactct atagcaattg attttcccca gaaagaagca aaatcataaa tcatataatc
    15841 aatgttttcc tctctcaaca aatctgagta atacttcgtt atagctttct ccctgtcaag
    15901 tgtttgaaca aaaaataatg atgttgccaa cactctttgt agcaaactat cctgtacaaa
    15961 tgcatttact ggataaccca tacagaacat gttatatatt tcattcaggt tatcatattc
    16021 tctgaattca caatcaaacc ctttgaataa atggctatat gtactactag aataacatat
    16081 gagcttttcg cctctcagag ataattcctt gagcatttca agatttggaa ttatatgccc
    16141 atagtgcata tatgagaaat aaacacactt acccattttt actctcttcc tttccctttt
    16201 ttctatattc taaaaaggca cttagatcct caatttcatg attgtcattg atattataat
    16261 aatgtttact tatctcataa atgtgaactt gctcattatc atttaatgat gcatttagaa
    16321 gatccaccag atccctttca tttctttgct cattaacttc tatcttgttc atatatttta
    16381 gagttgaatt tctaaataat attgttcctg tccccattat attgttaatg ctatttttag
    16441 gtttttcttc gaaataaatt aacttgtcat tctttacagt aactccatat gttttgctta
    16501 ttctggtctt gtcatcaaca taaacaatgc cagccagtat gcttaactct gaattaataa
    16561 agcatgagat aaaatcctta tattttggca tttcatagta ttcatcgcct aacataagca
    16621 taaaatcatc atctcctatt acgggtatgc attgctctat agcacctatt atgccattca
    16681 gattccgctg ttcaacaata acaagttctt tatgcgtact tttttttgct tccgctataa
    16741 cctcatcctt gttatagtta acaacaatga ttactttgga tatttcatct aattcattca
    16801 cataagatag ggttttctga agcagtgata catcttggaa cctaagcaga catttattcg
    16861 gacaatttaa atttgatatc ctggatcctt ttccggcaca tagtattata ctaatcacat
    16921 ataacctact ttcaactaca gaataataaa atttgtcata taataataat taccatactt
    16981 tagataatat aaattacttc atcatatata ttgaatatta actgttcagt ttgcttttgt
    17041 aatataatca agaccaatag taattgttct aacttcattc actaaaaaca ctgttattac
    17101 tgcattcttt ttttgcttgt tgattttaag gattctgctt tcttgtccct tcaaaggtcc
    17161 ctcaattact tttactctat ctccttcaat caatccttta gaatactcaa tacagtaatt
    17221 gtcattacat aaatctaaca gaaaatttat ttccgtttcg ttaacggcta ttttttcata
    17281 agacccatag tttaataact taaaaatatc atttgttttt ctaacaaaat ccataattat
    17341 tataagaaaa gctgcttctg caatacatga tttgataaaa acatatcctg ggaatattat
    17401 tcttgttact tcactgattt ctttgcctct tttaaaactt acattctttt taaaagtaaa
    17461 gggagtgaaa gactgattac ataagcaatt ctgtattttt gtgcatactt gctcttcttt
    17521 cccggttatt acatgcaaga tataataata tgctttttca tcttgatgag ttggcattcg
    17581 cagtaacctc taaaaattct gtatgttttt tgatatatat ttaatataaa taatatatat
    17641 aatataaaga ctatgacctt catactcttc cactgcaagc ttgctgcata tggattcttt
    17701 atgaattatt aaaatcttct aaactttaac atacaatatc tataatgtca atacttaatt
    17761 tccttgagtt tccgtacttt taaccaggtt catcatgcct gcagcttttt aacatcgaaa
    17821 acttaagaaa aagccttaaa aaaggaggaa tccccgcctc ttctgtggta aaattaaatc
    17881 aataaaaaaa ctttgctaaa caaaaaaaga aaggaatcct catggctaat tatactacag
    17941 atacttatgc aatgaaacgc gaaatcctca cattcacaaa taatttaaca aaaggactgc
    18001 atatcccaaa aaggaagttc ataaccgata tgagctatgg gatgctggcg tcaaacagct
    18061 accttctctc agatatagcg gataccctcc acgaggaagc aaaaaagaag aacactatcg
    18121 aacgcctctc gcttaatttt gcaaaagaga tacctgccca gctggccgct aattatcttg
    18181 ctaaagcaaa aacctcgcaa gcagcaatcc agcggtacac atcgatgaca gagatatagt
    18241 caagccggac ggacgcaggt ttgaaggtat cgggatagta agggactgat ctgagaggta
    18301 ttctttggaa gacaagacgg aaatcaataa aactgacact gagtgttttt agcgtgaaaa
    18361 ttctctcttt gagaaagtgg agaattttct ccgccttttt tgaagatttt tattgacaat
    18421 cacaaataac aaacagtaaa caacaagcct ttttgggtaa tgttgtgagg ttttcaggga
    18481 cactattata ccaaatgcca tccttgacaa gctgctgcac cattcccaca caatcaagat
    18541 cactggaagg tcttacagga caaggaacgc aatatcctca aacaatgccg aacaggaaaa
    18601 gcagtaaaga aagtggtcac ttttattttc cactttttgc actcatttat attggtatta
    18661 tcaagatata ctgtagcaca atacaaccct cagagaagca cctgttcaga ggaatttcat
    18721 taaccgcctt tacaggactg ccgtagtaag tgatttgaca tatgtgaggg tcgggatgag
    18781 atggaactac atatgcgtac tggtggaccc aacaggaaga tcataggata cagcacgagc
    18841 gttaacaagg atttcaggct tatattcaaa gcattctcga gaattaaagg atatctgtca
    18901 gacatacaga tattccacaa tgacagaggt aacgagttca agaacaatgt gatagaggga
    18961 acttctgaag cgttctggaa agaagggtca ttacgtagaa agtcagcaca tatgacaata
    19021 cggttgcaga agcgacattc aagatactaa agactgagtc tttgaaagga aattggttca
    19081 acaacttcag gatacattca ttattggggt atatgagccg tattgaatgt tgaaatgata
    19141 ccttaagaag ttgcctaaaa aagtgttgcc agtccacctt ggatattatc acaggtttta
    19201 cttctgaaaa cctttctttc tttggatcag ataaataatt atatggctca catctcactc
    19261 cgagtcgttc ctatgtaacg acagtaagca tccttgataa tcgtaataag gtttcctcat
    19321 tcctactata tagaatagcg ccgtcaaaaa gcgagtcttt tataagaata atatatcttg
    19381 tattttaatc acagaatttg atggtccaca ttttttctat cctttccgac tagtttatgc
    19441 tctttagctg ataataaatc atatgattac tattccacac aagctgctgt accggtatct
    19501 gactaataaa atacttttcg aaattagcat attaagtata cttagtcaat aaatatctgg
    19561 ttttgttctc tttttcgctt acttcccgtt tttttcttct tctaaaacct catttatagc
    19621 tatcgataac tggtctgggc aagatgtctt cttgtgacca cattttatgc cttttaataa
    19681 gctaattgct ttttctgttt ccataccttc taccagcacg cctataccct gcagattccc
    19741 gctgcatccg cctttgaatt tcacgttatt tagttttcca tcctgtatat caaaagttat
    19801 ctcgcttgaa catgttcctt tggttttgta tttatacata tattccccta taattcaatc
    19861 tctttagtta atatttttct tatcgtatca acatatatga tatgttcctt ttccagaact
    19921 ctttttgcta atgattctgc tgtatcatcc tcaagaaccg gaacctttat ctgattaagt
    19981 atctttccgt tatcatagta ctcatctaca aggtgcacag tgcatcctga ttctttttcc
    20041 ttagcttcaa gtactgcttt atgtactctc tccccataca ttccctgtcc tccgaattta
    20101 ggaagcaaag caggatgaat attcagaacc ttgtatcttt ccactaattc cttaggcagc
    20161 ttcttcatgt aacctgcaag aagtactaaa tctatcttat accttgctaa gagattcaaa
    20221 agcttacctg caaactcttc tttgtattta tactcgctta catggtaatt atcaatcttc
    20281 tcattttctg cacgctctaa agcatatgcc ttactgttgt tggaaattac cagcacaacc
    20341 ttcgcatcaa tcttcttatt cttacaagca tctataacag cctgcatatt actgcctccg
    20401 cctgaggcaa aaacacaaat attcatcatg caagattata tcatcatatc gtcatggctg
    20461 cacatacaga taatcataaa tattgccggt aatgtcaatc atatattctt ttatatgctc
    20521 tttcttctca ttttttgcta taacagttat agtgtactta tcttttatcc tgtttccctc
    20581 tgtatctata gtatcgagac aaagcgggag tatgtagctt tttgtcaatg tatagccatt
    20641 attccataac ccttcaatat gcatatcttt tggacacata tcataacttc ctaaaagatc
    20701 agcatagtca taagtattac catatccatc ttcatataca tggcttttaa gttcctctga
    20761 gaagattaca gttatatccc ttgctcttcc tgctactgat acattaaact gaacattctc
    20821 aagacctgta aatctttcat ttaatcccca tatgttccag gcaccattaa gtgatatatc
    20881 agtaatacat acatcatact gtgattcatc cggttcctga tatggaatat cctcaggttc
    20941 atctgatatt atctcttctg gtataacggg attagcaggg ttatatgggg cttcttcttt
    21001 gtaaaggtcc actgagcagt aatccccggt tatatacaca tcattaaata tactcttaac
    21061 ctcaatcaat actcttactg tatattttgc aggcataata tactggattg gtatattaat
    21121 ctccagctgt ccgcttcgtt catgtggctg cagtgttata ctgtttcctc cttgtacaga
    21181 tagtttaata tactcaattt cctctaatgg tttatctact gagaaactga cattcacata
    21241 tcctttttta tagacatcag tatcagaata aacaagtctt tcctgtgtga agcatgtatt
    21301 tagggataca tcaggagtac ccatagaagg cactttgaaa gtcgcgtaat acccgttagt
    21361 tttgtatgtg tatatagaac ctgtactaag ccatgaggcc ccgttttgca cccttgtctt
    21421 acttatccct atatcatttg cagtaaaatt tacagctcca tgcccaatta gattagtatg
    21481 tagcatatat ggtttctgca ctgttgattc cagcctgtcc catgattcag ttgctccagg
    21541 aatatccgta tatatccact gatttgcatt aacaggtccc atttcagtat tctgatagga
    21601 tatattggta aaaagatctt catcatatgt ataccctaag aactggtatt ctcccgctac
    21661 acatgggtta tttgccacat tatatgggtt accataaaca atatatccgt aatcacgata
    21721 cattgtaaaa gatactgtat agttaccatt tatgctttta acaggtattc caagatgctg
    21781 tatccataaa ataaactctt cctggtttgt aggagtattt gaacataaag ggcagccgaa
    21841 tatatcctca tatactgttt cttcgtatcc gcaagttata catctgtata caacagcatg
    21901 cccaaaatca gtatgctccc cgatattcga tgtcctctgc atatcgtgcc catgacatag
    21961 acagcatggt ctgtatgtag taaaccgggt ttcagtatac ccgcaatctt tacaagtcct
    22021 ggtctgtctg tgaccttgtg cttcatggac gcttgaaact tctgaccact gtgaaaaatt
    22081 atgatatccc ctacactgac agcactcagg cagagttaca tatgatgtag tgacatgacc
    22141 gcaatcagag cacatgccag ttttaaggtg cccgttcggg gcatgctcat tagatacagt
    22201 atatgtataa ttatggtagc ctctgcaata gcagcaggtt ggcactgtag tatagtaata
    22261 ttcctcgtgt ccgcagtcta cacatctttg cgtaaccaga tggccatatt ctgtatgagt
    22321 gtcagacacg gagtaatagt aattatggta tcctctgcat tcgcaacagt gcggtatagt
    22381 agtataccca gaatcaataa cctctccgca cttatagcaa tatctggtat agaaatgacc
    22441 taatcccggg gtatggtaat cagatatttc cgttacatac acatgatcgc aattacaaat
    22501 actacatgac cataatgtgg tatatccact ttcaataatt tcgccgcact ccttacatgt
    22561 aattgtgtag tagtggccta aaccatatag gtgttcctgg cttatctcta tgggacctgg
    22621 agtatgtcct acacactgac agcagctgta atcaatatca tagtatgatg tagtgtgtcc
    22681 gcaatccaga catctaaggg taaatagatg cccctgagct gtatgaggcc ctatagagaa
    22741 tagacagtaa ttatgatttc ccgtacactg gcagcatgtt gatatatatt tgtagcctag
    22801 aatggttata agaccgcagt cctggcatat atatccatag gcatgtcctc ttgaggtatg
    22861 ctcatcagaa atatatgaag cattcatgtg ctctccagta agttcatatg tctcacaata
    22921 tgctagagca tttatacaga tgcttagcag taaaattatt gtaataaaag acaggatacc
    22981 ttttttcata ttatcttctc cattagtttc cgttatttac tattttctac atatacttct
    23041 tctttttcat ttacttagat gaaaaagtaa aattaccaat tacgttttcc atcgtgaaaa
    23101 atacatatct taattcatca ttagagttat cctctaacag gttatatgca tcatgagtat
    23161 ctaaagaacg tataaaatca tacttcttca ttatgtaatc agctatatca tattggtcat
    23221 attcagataa agctgcacat atagcatttt ttactttgga tttatagtaa gaaacgggta
    23281 taccgtcttc ccaatcaagt gattttaatt ccagttttaa aaacatagta tcataacccc
    23341 actgtttttc tggataccct gtttcacctt caaaaaacct taaagcgaac atacagtaag
    23401 aggggttgga atacaggctg gatttatgat atctgatctc atatgtattt ttagtctcat
    23461 ataccctttt ttccacaact gtataaccta tatcatcctt aacctgtaaa agcgtgtgtt
    23521 ttatagaatc agataattca tcaaaaccaa caccaacata tcttatattt tcctgttctt
    23581 tattactcag aaacgaacag tccattctta ctgaggtgtc tcttttgctt ttgtccatga
    23641 cctttataac cagtgtacat gcctgtgctc tggtaagggt ttctttgtac ctgaaagtaa
    23701 agtcttcaaa cccattaatg atacctgcac tgaagcattt tttaatatgt tgtatatatt
    23761 caacaggtgt atacatacct aaatcaaacg ggtacacttc ttctgtatta tattcaggtt
    23821 cctttaagta tgtcatggaa ttggatatga gtacagctgc ttgtcctctg tttatatctt
    23881 ttgtatactc agcaccaaat ccttcaagca tccctatttc ttcagcttta tctatatagt
    23941 tttgagccca gtagtctttt gaattttgga tatcaaatcc taaagctttt attatcattt
    24001 ttataagctc atctgttttt acattacctt caggtttaaa tgtgttatca ggatacccgt
    24061 ctataatatt cataccatac agttggatga ctggttcaaa ataccactgt gatgattcta
    24121 tatcagtaaa tctcgaaccc gaagcattta catttatttc aagtaacccg cttaacaata
    24181 tgaaactgat gattaatagt agtgatacaa gtctgttctt tgcaatattt ttcatggcac
    24241 tcctcttcga atgccatact aatattattg ccataatatg tcaactgaca aatgtgtatt
    24301 tcttttaatt acattaaatc gtatgaaccg gaagcatcca tactgaatct gttaatggga
    24361 gaagttcctt tgtaaaacag attaatgcac cagtaccgat tttttttacc ggattttttt
    24421 caagacaatg aaaagctttt accatcgact ttgtcggatt acttgaagtt ttaatctcta
    24481 tagggtagag gtaatcaccc tcctctacta acaaatcaat ttcatttttg tccatatctc
    24541 tatagtaata tatcaatggt tttacaatac cgtcattgta ataactttta agaatttctg
    24601 caaaaacata actttcaaaa atgtgacccc acattgctcc atttaccatc tgttccggag
    24661 tattccatcc aagtaaccag caagcaagtc cagtatcaag aaaatacagt ttcggtgatt
    24721 ttactaacct cttattatag ttgttataat atggttctag aagataaaca agcccgcttg
    24781 attccagcac agatagccat gaacggactg ttttatcgtc tttaccgcat atttccgata
    24841 ttgcagacat gttcaatact tcccctgtgc gtgctgcaac tgcttttaca aatttaataa
    24901 atgcagattc atcttgaata ttaagcacat cctttatgtc tttttctata tatgtctgaa
    24961 aatatgaact atagaaatct gaccaatctt tcaaatcact ctctgtttca tatagctcag
    25021 gaaagaaacc tttgtggata tatgatacga atttaagata gtcatattca acagatcctt
    25081 tttcatcatt catcatatct tctaaaatag gtagaaatgg ctttctccat gaacttcctt
    25141 ttaattcacg cattgacaga ccaagcattt ttataactcc tgtacgaccc gccagactgt
    25201 ctgttacgtt tttcatcaat ttcatgcttt gggaaccagt aagataaaac tggcctttta
    25261 tctttttttc atcaacaata tctttaatat actcaaaaag ctccccagct ttctggattt
    25321 catcaacaat cacgggaggc ttatgaagat caaaaaacaa agatgggttt tcctttgcac
    25381 tttctctgac caacaaatca ctcagagaaa caaaatttat attaccacac acatttttca
    25441 acatggttga tttcccgacc tgccgtgctc ctgttaagac aataacagat tttcgctttg
    25501 caattctctc tacaaccggt tctatatgcc tgtgaaaata cattgctgcc tcctttatgt
    25561 cgatattttc ggaattcaat tccgttatct acgaagtttt gcaggaagtc aatatttagt
    25621 acctttttac ttgctgataa aataaatcct aatttatcgt tctttactca caacagaata
    25681 caaataatac tccataaata ttattcaata aaaccctagt aaagactata tgtactgacc
    25741 tttgtaaagt gggcaaatag ttaaataaaa gctaagagca attgcttggc tttaaggatt
    25801 tgtatctgta atactgtttt ctattatttc aaatttatat tcatctaaac cgtatatttt
    25861 agaaaaatca aatgtaaatg tatattttat gtcttttaca acaaatttat tgttttgcat
    25921 ttcatatata acttcaatat ctaatacttg aacaagcatc cagttctctg tatttatcaa
    25981 aaaaagaacc gtattttctg taattccatt attcacaata tttgaagtca agtattctcg
    26041 gtaatcaatt ataaaattag gtttaactgt atcagaggat atctttatta ctatctgatc
    26101 attagtattt ttatttttta tagtagcaag acaagcttgt tcatcttcta tatatacaac
    26161 atcatattca agtatacttt cgataacatc ataaaccgaa tttgtagaaa acggaattgc
    26221 aacaaaatcc ttttcattgt atatatgtac aaatcttttt cttccatatg ttgctcttgg
    26281 atcgtttata aatgcaattt cattttctcc atcatcatta aaatccttta ctcgaactcc
    26341 tacgtatctc ccccatatat tatcagttaa ataaaatatt tcattatttc ttaaagctaa
    26401 aagcattcgt tctccagatt ctcttgcaaa gtataattct atcagattta gaggatagtc
    26461 atttggcaat atatacaatt cattaacata aatatatttc ctttcaagat ctatcccatt
    26521 taaataatat acattatcat ttaatatttc attttctttt atgtcac
//
LOCUS       DAOSXM010000024        26485 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2111
            NODE_1171_length_26485_cov_9.304238, whole genome shotgun sequence.
ACCESSION   DAOSXM010000024 DAOSXM010000000
VERSION     DAOSXM010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864178
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 26485)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26485)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:24:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,261
            CDSs (total)                      :: 2,229
            Genes (coding)                    :: 2,218
            CDSs (with protein)               :: 2,218
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 3 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26485
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1171_length_26485_cov_9.304238"
                     /isolate="AMR_MDS_2111"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(23..1696)
                     /locus_tag="PLQ68_04985"
     CDS             complement(23..1696)
                     /locus_tag="PLQ68_04985"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HXK72037.1"
                     /translation="MYTEVSSIEMLRKAIEEKKEFIRINEGFYEIDEPLILDNVNNLK
                     IVGNGNVVISGAKKIMNKSTYPNSLFVNNIRRFKSSAYKVEASAWNRCEREDFIFHKS
                     VEKYDSRDIFSGFLTDHIELYKEKPENIEFVFNVGWTQNMANGKSSEKNGDKVLINLD
                     YNQFKLLQTKNVTRVGAPTELKNLKYALKKATFYHDKNEGKIEYIPTDDEVKNGYEVM
                     YPVAEQLVILKNCKNICFENITFSHTTWEYPSKKGFCEMQSTIYKNLNEGLDSTKREI
                     PVSAVDISYSTEVTFNKCEFSHLGATGIYLGRGCKNSKIIDSKIFDIAGCGIIVGGFE
                     VRDAHPDDEDSINYKTLIENNKIFDIGVLYSGACGILAGYVNSIKIYNNLIYNIAYTG
                     ISLGWGWGTIDPIAHLIFNSSVKEPFTKPTILKNNHVKGNEIHHIMTSMHDGAGIYTL
                     SNQDGTIIEDNYIHDNGNNEDEPVKKIYIKKSYLLGVTTKWGKISKRKGYPGGIYLDE
                     CSAGITVRNNRIENVACDYYYHDTGVPGIFETNKFDVEPLDNPILSFDE"
     gene            complement(1686..2300)
                     /locus_tag="PLQ68_04990"
     CDS             complement(1686..2300)
                     /locus_tag="PLQ68_04990"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXK72038.1"
                     /translation="MHILKLTSQLLSSNCYIVISDDKAAVIDPSIEPKIIIEKIKVYN
                     CVIDKIIYTHAHMDHIIYGDDLKALTGAQIYAHPDDWLLYKDEYKNGAILFGMHKTFN
                     ENNINLIDKMVLKHGNVDLNIIHTPGHTMGCICIKAENFVFTGDTIFYESIGRTDLST
                     GDADLLFDSIKNKLFTLPDETIILPGHGIKTTITHEKENNPYVY"
     gene            complement(2302..2766)
                     /gene="dtd"
                     /locus_tag="PLQ68_04995"
     CDS             complement(2302..2766)
                     /gene="dtd"
                     /locus_tag="PLQ68_04995"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013749994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HXK72039.1"
                     /translation="MIAVIQRVKESYVKIDGIIVSNINKGLNVLLCVTLEDTEEDIIY
                     IADKIVKMRIFPDSNDKMNLSLIDIKGDILVISQFTLAADTKKGNRPSFINAAKPQKA
                     DEYYNQFISYIKNTYSIDVKTGSFGAQMHVGILNDGPVTIIIDSKENRIKKY"
     gene            complement(2763..4946)
                     /locus_tag="PLQ68_05000"
     CDS             complement(2763..4946)
                     /locus_tag="PLQ68_05000"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004617605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HXK72040.1"
                     /translation="MENYELLYAKLIDEIKLYNPNSNFDLINRAYNIALEGHIDQKRA
                     SGEPYIIHCIEVARIVATLELNSKSIAAALLHDVIEDTKYTANDIKNMFGSEVSLIVE
                     GVSKLTNISLQTQKEQQVENIRKMFMAMSKDIRVIIVKLADRLHNMRTLEYKTKKSQF
                     EKSKETMDIYAPIAHRLGMSSFRIELEDLSFMHLYPEAYADLSEKMEKNKKERYELID
                     NIRNELFIDLKNNNIEGSVEGRVKHLYSIYRKMVNQNKTLEEIYDIFALRIIVNNIKD
                     CYFALGMLHENYKPIPGRFKDYIAMPKKNMYQSLHTTLINRNGKPFEVQIRTWDMHRV
                     AEFGIAAHWKYKQNIDNADQSSSLEWISQWMEWQKETKDEDEFYSNLKNDLFDMEVFV
                     FTPKGDVINLPYGSNPIDFAYHVHSAVGNKMIGAKVGGKMVPVTYTLQTGDIVEILTS
                     NTGKGPSRDWLKLVKTSQARTKIKQYFRKERKEENIEKGKDQLNKEIKRQGFSPVDLL
                     KPQYYEKVLERYNFQELEDIMCAIGYGGITSNKIISRLREEYRKDHKEDHYPIIQEIT
                     SNYKSSKGNEGIIVKDIPNCMVKISKCCNPVPGDEIVGYVTRGRGVSVHRSDCINIIN
                     DKEFTDRKIEVYWNMNSDESYETVLQIKAYDRPNLVTDIMKLLSELKIKCNGVEAKLS
                     KEAYADIRIRVMMHNKDEISELNKKIRKITGVFDIIRISKRQKIR"
     gene            complement(4949..6958)
                     /gene="recJ"
                     /locus_tag="PLQ68_05005"
     CDS             complement(4949..6958)
                     /gene="recJ"
                     /locus_tag="PLQ68_05005"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HXK72041.1"
                     /translation="MIFNNEWNIDDTADITNCTIAEKILYSRNIKDIEKYLYPKLEHM
                     HNPFLFEYMEKAVNRILEAVKNKEGILIYGDYDTDGITATAIIYKYLLQNTANITYFI
                     PDRKKDGYGLSIDTLEEILNEEIGLVITVDCGITAVDEVSYMNDLGIDCIITDHHTPL
                     DELPDAYCIITSKLNNCLYPFKHLCGAGIAFKLIHAICITLGQGYEYAKYIDLAALGT
                     IADMVPLIEENRVIAKIGMDLLSKSENIGIRSLISKSYPNGIDKINSSQLLFNIIPKL
                     NASGRMDSAEHSIRLLITDDLFECDMLSLNLIELNESRQVLQREITEQVFREIDENSE
                     IKNCKILVLSNEKWDRGVLGIVASSIIEKYNKPCILMEILEDGSAKGSARSVGGFNII
                     KAIDYCKDFLSSYGGHKMAAGLTIKKDNSSNNEKIKEFAKMINQYADDIKFEVKDNKT
                     ICPDFIIDINDLTLKNAELMSLMEPFGCENELSLLMLKEIEVYEHKLVGKNKDHLHIK
                     FTKNQKLISTILFRGEAYEKLLIPNQKYDIIFKLSCNTVKDSNSKSLSIRKFINCEVV
                     DIRFSDIKQNLINELIKYTTTSISIDRNNIADLYNTIINKSVPCEFNLNELTNVSTFL
                     ICLDILKELGIIEYNNINFQKIFVTKLNNSEKKDLMKSKTYIKVT"
     gene            complement(6955..7965)
                     /locus_tag="PLQ68_05010"
     CDS             complement(6955..7965)
                     /locus_tag="PLQ68_05010"
                     /inference="COORDINATES: protein motif:HMM:NF026253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HXK72042.1"
                     /translation="MRKSKFAIVLFLLIISLLILSSCSIVKFPVYLYGLIDTEGNYIV
                     EPTFTGIKIGKEGLYPIRQGYNEYYKWGFCDRTGEVVIEPQYKDASMFSEGYAPVKLY
                     DDESLWVFIDKDNNIVFDKEFNYALPFSEGLACVQSSKRDATYNLWGYIDNTGEYVIE
                     PQFQAAASFSEGLAKFQTGSGVAGLCGYINMQGEVVIEPQFSYAGLFSDGLASVKLSL
                     DQETNDAGYIDKKGNIVIPLQYYNTSTFKEGLAPVLEGESFKDALYGYINKDNEMIIK
                     PKYYMAYSFSEGLAAVKTDYYENSGWMYIDSEGKLVIKNDYSSASSFIDGIAPVGMVE
                     YR"
     gene            complement(7965..9740)
                     /gene="aspS"
                     /locus_tag="PLQ68_05015"
     CDS             complement(7965..9740)
                     /gene="aspS"
                     /locus_tag="PLQ68_05015"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HXK72043.1"
                     /translation="METIKGFKRSGYCTQFNISNIGKEVVLMGWVNKSRNLGSLIFAD
                     LRDRTGIMQIVFRHENNQDLFEKATLLKSEYVIAIKGEIKEREDKNSQIPTGDIEIEV
                     KELRILSESSTPPIDMADNTSTNELLRLKYRYLDLRKSKQQQYLFMRSNISKIARDFF
                     ANNGFIEVETPVLTRSTPEGARDYLVPSRVNQNKFYALPQSPQLLKQLLMMSGFDRYY
                     QIVKCFRDEDLRANRQPEFTQIDLEMSFVDIDDVIEINEAFIKELFKNTLNIDLITPF
                     KRMTYEEAMSRFGSDKPDLRFSMELKDISSKVENCDFKVFSDACKNGSVRGINAKGCG
                     GFSRKEIDKLTDFVKDFGAKGLAWLKVSDTLEISGSITKFFNEEQLTEILKIFDAKAN
                     DLLLFIADKNQVVFDSLGALRVEIAKRLNLTDNSKYEFLWVTEFPLLEFSEEDNRYYA
                     KHHPFTSFMEEDYEFLRTQPDKVRAKAYDIVLNGEEIGGGSIRIHDYKLQEEMLEILG
                     FTKEKAEESFGFLLNALKYGAPPHGGIAYGLDRLVMSITKTENIRDVIAFPKIQSGAC
                     PLTDAPNTVDIKQLDELNIYLKEKE"
     gene            complement(9749..11011)
                     /gene="hisS"
                     /locus_tag="PLQ68_05020"
     CDS             complement(9749..11011)
                     /gene="hisS"
                     /locus_tag="PLQ68_05020"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HXK72044.1"
                     /translation="MKISIPKGTKDILPGEIEKYKYVQTEFSKLLENYGYQQIRFPVF
                     EYTELFQRGVGDTTDIVQKEMYTFLDKGGRSITLRPEGTASVVRSYIENGMSSQPQPI
                     KLYYDITAYRYENVQKGRYREFNQLGAEVLGSDSSNADIEIMVMLNALFAKLSISNVQ
                     LNINSIGCKSCRDKYNQMLKDYFSDKISNMCDDCKKRYEKNPMRLLDCKNERCSIISK
                     DAPMLLDNLCDDCKNHFDLVEKGLKEMNVPYIVNKRIVRGLDYYVRTVFEFIAEGIGA
                     QNTICAGGRYDGLVSELGGNDTPGIGFAMGLERLIDVMEKSGFKLPDEKKPVIFFAFI
                     GEKAYKAVRKMANDLRNKDIYCEIDVTDRSLKSQMKFADKKNALYSVVIGDNEIDSNK
                     AIIKNMLTQETKEVSVDSITRRIIENKI"
     gene            complement(11166..12260)
                     /locus_tag="PLQ68_05025"
     CDS             complement(11166..12260)
                     /locus_tag="PLQ68_05025"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HXK72045.1"
                     /translation="MSEIRNNSIKKNNKPKTRKLSNKGIIIISVAALLILCIIISLLI
                     FYDIMHNSIYETPDATSSQNIEIITLPPLETESEQPTQTEEPTEDVKYKPVDDIEYIL
                     LFGLAEYNLADTIWIAVINKTDNTVNILSIPRDTYLRYSDRNNPEHWKINSYYNNYKD
                     DIWIKTDDSTSSMNLLGACEKLLNIDMDHYVRVSYDTIERIVDAMGGIEYDVPFDMHY
                     YDYTEGHELFIEIDEGHQVLDGENVVKFLRFRQGDGYEYISDIARIERQHKMINAILK
                     KALTLSNISSVIDVAKNNIRTNISKDKLTSLITFSLSLNTDNISYYTLKGEEKMVDTG
                     LIDDEGKPNKKSFYITKPEDIKQQIISISE"
     gene            complement(12262..13524)
                     /locus_tag="PLQ68_05030"
     CDS             complement(12262..13524)
                     /locus_tag="PLQ68_05030"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF010697.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HXK72046.1"
                     /translation="MKIFLDDCISFAKNDDYYKMLENAKKANELLKSKKGRGSDYLGW
                     LNLPNQITTDEIKDIKQTAEKIKNQSEVLLVIGIGGSYLGSRAAIEALTDTKNIEGTE
                     ILYVGNNISGKYIESILKYISNKDYSINVISKSGTTTEPAIAFRIFKNEIEKKYKDKA
                     KDRIFVTTDKEKGSLRQIANDLGYKTFVIPDDIGGRYSVLTPCGLLPMAVAGIDIDMM
                     LKGAKYSMDNHEEECLQYAAARNLMYSIGKTIEITVNYEPSLHYLTEWWKQLFGESEG
                     KENKGIFPSGVDNTTDLHSMGQYIQEGIRNIFETVLFISDDMSKISIPESSDNLDDLD
                     YIKGKSLQYVNEKAMQGTKLAHVNGHVPVILLEIEKMNAYNYGSLVYFYEKACGISGY
                     VLGINPFDQPGVEAYKKNMFKLLGKSGY"
     gene            complement(13508..14164)
                     /locus_tag="PLQ68_05035"
     CDS             complement(13508..14164)
                     /locus_tag="PLQ68_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HXK72047.1"
                     /translation="MINEIELLENNKIYVAGVIAKTPVFNHQIYGESFYNVILEVQRL
                     SKQTDMVPILISDHLLDIDLLKPKVMLEVHGQVRSYNNIDKADKKRLVVNIFAREAKI
                     IESFKDIDNFNDVFFDSFICKDPNFRVTPFGREICDLLVAVNRAYKKSDYIPCICWGR
                     NARYCENMAIGTHLKIWGRFQSREYEKKDHYGNIITKTAYEVSVIKLEKVEDDNENIS
                     "
     gene            complement(14289..14864)
                     /locus_tag="PLQ68_05040"
     CDS             complement(14289..14864)
                     /locus_tag="PLQ68_05040"
                     /inference="COORDINATES: protein motif:HMM:NF021561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2284 domain-containing protein"
                     /protein_id="HXK72048.1"
                     /translation="MKKVIKTLMKHAEDFGFTHSGELNLDSLVFMPEVRQMCNSDLCK
                     SYNKNWMCPPAIGTLENVALRVKNYNFGMIVQTTALMEDEFDYDTIHNTEIKHKNNFM
                     KLVDDLKSNYSDILPMSAGTCSICNKCSYPDEPCRFPNKAIGSMESYGLWVSKVCELS
                     NIPYYYGKNTLTFTSCYLLNLHNIDILDKIT"
     gene            complement(14911..14987)
                     /locus_tag="PLQ68_05045"
     tRNA            complement(14911..14987)
                     /locus_tag="PLQ68_05045"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14951..14953),aa:Pro,seq:ggg)
     gene            complement(15011..16150)
                     /gene="lgt"
                     /locus_tag="PLQ68_05050"
     CDS             complement(15011..16150)
                     /gene="lgt"
                     /locus_tag="PLQ68_05050"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HXK72049.1"
                     /translation="MRSTVSFPNLFGGLELSVNRVAFNIGRFPVYWYGLLIAIAVCLA
                     VVLVLMAAKKYGLNEDDLLNICLIGIIVGVISARLLYVLGRLDQEWTFGDVLNVRDGG
                     MSIFGALIGGFAATAIYCKIKKINFLKLADLVVPYIALAQAIGRWGNFINKEAYGVPV
                     KPSSILSSLAMTSMKIGGNVAGSIEEELLQLGLVDTSLIANVNETTFNYIGVHPTFFY
                     ESILNLALFLVLILVARNGKYFNGRSLCIYAASYGLYRFFIEGIRIDPLYLFNTDIKL
                     SRLIALISAIFFGSMLILISILRSKDKKLFLKSLIDEEDILVGQSDYKELLVDRDTFV
                     PEDQPVANTSGQSSNSDKENSEINKKTITDDISTDESKTESVDKK"
     gene            complement(16205..17134)
                     /locus_tag="PLQ68_05055"
     CDS             complement(16205..17134)
                     /locus_tag="PLQ68_05055"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004162.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HXK72050.1"
                     /translation="MNVITSNEGYRFTKYNVIALGNFDGIHIAHQKVIKTAVEIAKKN
                     SLTSLVLLFNKHPISVLNDSAPKLLTTLEQKIKILEQLGVDYVYIQEFDVKFSTLEPD
                     YFIIKILIDKLLAASIVAGFDYRFGKGAKGDTQYLKEICVKFKTDVTIVDKVDFYNEP
                     VSASRIRNLLDEGDIEKANKLLGRRYSIEGTVVHGRGLGSKIGFPTANIDISKNIKLP
                     GSGVYLTKSSIDNELFDSITNIGSNPTINDYLTDRISCETHVIGYSSLNLYGKEIVIQ
                     FKRKIRNEQKFDSLDQLRKAIENDILLVKKQLE"
     gene            complement(17115..17960)
                     /gene="truB"
                     /locus_tag="PLQ68_05060"
     CDS             complement(17115..17960)
                     /gene="truB"
                     /locus_tag="PLQ68_05060"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HXK72051.1"
                     /translation="MLKSIFINQKIGHTGTLDPLAQGVLVVCIGKATRLADYIQSQTK
                     TYQAELLLGFSTDSYDITGKITDVYDISDITDEMIRKTILSFKGIQNQKPPIYSAKKV
                     NGKKLYQYALQDKKVDIKDSVINIYDISLDSITDDFYQEIKVKKVIFTIYCSKGTYIR
                     SLCNDIGEILESGGTMSNLTRKSVGNFKTENSYTINEIIKFHENKRLNDIFTPIEDAI
                     FLDTLIIKDEDLKKYLNGIKIQTNYKSNEYIIKDEQKTIIGIGKVDDFGILKGKVMLY
                     ECNNQ"
     gene            complement(18008..18997)
                     /locus_tag="PLQ68_05065"
     CDS             complement(18008..18997)
                     /locus_tag="PLQ68_05065"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HXK72052.1"
                     /translation="MKLTKIANQLNKDIESNKNFLILPHVMADGDAVGCCIAMARYLV
                     SLGKKAVVLMEEKIPYIYSFMQNSADIRIYSQESEYNNYDVYIAIDTGDINRLGKRKA
                     IFENKLSYNIDHHKTNNGYAKYNHVNEDVSSSGEIIYDLLSLLKAQIDHDIAQAIYVA
                     ISTDTGGFRYQNTNKKCFKISSELMNYGINIEKISEWIFERTNLTKLYLFQKTIQNIK
                     LHCNNKVAVSTLRTEEYLNLDVNDEDFEGLVNIVKNIEGVEVGVFLRERPNKEEVKGS
                     LRSNTDKVDVSVIAQLNGGGGHARAAGLISKKNINEVYENIIMNLNELFECMQ"
     gene            complement(18963..19325)
                     /gene="rbfA"
                     /locus_tag="PLQ68_05070"
     CDS             complement(18963..19325)
                     /gene="rbfA"
                     /locus_tag="PLQ68_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004814152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HXK72053.1"
                     /translation="MKQKRVERLNSEFKREISSILAEEIKDPRLTAIVSIVNVNITND
                     LSYADIHVSMLGDEKSKKDSFTAIQNSAGYIRKLLGQRMMLRYIPQLRFKYNDYIEQG
                     IRISKLIDEINKDSKPTE"
     gene            complement(19322..22294)
                     /gene="infB"
                     /locus_tag="PLQ68_05075"
     CDS             complement(19322..22294)
                     /gene="infB"
                     /locus_tag="PLQ68_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HXK72054.1"
                     /translation="MGKKVNELAREIDKKASEVLRKLDQLGISCTVNTVLTDEQEQMI
                     YDVYRIKNTSAKQNPKVVTVTSPDSTKTKTVVIKAPEKQIDKSKSDDSVEKPMVVVVK
                     TTKGGETKSTIKHIKQEPENAPEPTKEKEKPQEKKVVVVKTPEVVVPDKKNEEVKPVE
                     SVKVESQIKETKAEQPSVEKKPEQQTVEKKPIENQKQTKEREFTKEEKKEKTYREHKE
                     YKNNTYIPGTRSDKPFVHKDNRPDKGYAQKTNRPDKPINRGERKPGYNQSNTSQGYRT
                     RPAGTFPSSQGYNKDKDQSTDRNRQGYKKPVDKDKSVEEIRVKADKIRVDKKELLKKD
                     QEKVLNKDNKKEAAVSKGITAKQSKYDKREIITPKSAIDTKSLTEDEIFTTFYRDSAP
                     KRRRKKRENIPAKIEVLKFVKLPPEMTVKEFAEKIKKTSSDVLSKLLLMGSDIMSINE
                     NIDFDSAALIADEFGITCEMESIKTEEDILFDDEAEDKIDDLEERPPVVVVMGHVDHG
                     KTSLLDYIKNSNVAEKEAGKITQHIGAYRVKINERYITFLDTPGHEAFTAMRKRGADV
                     TDIAIITVAADDGIMPQTVEAINHAKAAGVTIIIAINKIDLPAANPDRVMQQLMEHNL
                     VATKYGGDTECIPVSAKTGQGIDDLLDTILLSADILELKANPNRQAKGIVLESRMEKA
                     GTVTTLLIQRGSLKKTDYIISGTSVGHIKALYDDRGKNIEGAGPSMPVEILGLDSPPD
                     AGEMFYAVDDEKTAKSLAERRKHERQVIKTSHKLTTLDDLYDQIQAGNVKDLNIIIKA
                     DVQGSVEAISDSFKKLSTDEVKVHIIHGAVGTISETDVSLAEVTNSIIIGFNVRPLGN
                     VTEAAKSAEVDIRLYSVIYDAIEDLKNAMAGMLDPKTKEVVYGHAEVRNTFKITGAGT
                     VAGCMVLDGKILRNTNVRLVRDGITIFDGALSSLKRFKDDVKEVAAGYECGMSIEKYN
                     DLKVGDIIECYKVEVIKE"
     gene            complement(22295..22582)
                     /locus_tag="PLQ68_05080"
     CDS             complement(22295..22582)
                     /locus_tag="PLQ68_05080"
                     /inference="COORDINATES: protein motif:HMM:NF013417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L7Ae/L30e/S12e/Gadd45 family ribosomal protein"
                     /protein_id="HXK72055.1"
                     /translation="MIGLAIKARKCVTGADLCLEYIKKDKVRLLFIASDCTENTKKKV
                     VSAAEKKSLETFYTFSKNQLGDITGKEEISVIAITDYNFARGIREKITKEN"
     gene            complement(22593..22865)
                     /locus_tag="PLQ68_05085"
     CDS             complement(22593..22865)
                     /locus_tag="PLQ68_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003491326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HXK72056.1"
                     /translation="MKKSKKTPLRMCLTCRTMKPKKELIRIVQKDDGSVDMDITGKMN
                     GRGAYICDDIQCFEKARKSKILNRNFSATIDDNLYNKLEEYINRTK"
     gene            complement(22852..24057)
                     /gene="nusA"
                     /locus_tag="PLQ68_05090"
     CDS             complement(22852..24057)
                     /gene="nusA"
                     /locus_tag="PLQ68_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012447898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HXK72057.1"
                     /translation="MKKESSSNLGQNTDLIDAIRQIERERGIDSKTLIEAIEAALASA
                     YKKHTKSEQNIEVTMDVNTGEVKLFKVKNIVDEIVDEENEMLIEDAKEYADVEVGYCL
                     KFEEEMLPREFGRIAAQTAKQVILQRIKDAERVKILDIYENKLKEIVNGVVRRIENDT
                     VYLDLGNTEAILPKKEQVPSERYGINFKFKAFVFDVKKTSKEPKIFVSRTHPDLVKRL
                     FEMEVPEIQSGIVEIKDIAREPGARTKIAVYSSDSNVDATGACIGHRGIRVSNVVEEL
                     RGEKIDIIEWSANPAQFISSALSPADVSRVDVIEVENSNGDKEIKARVTVPDNQLSLA
                     IGKEGQNARLAAKLTNWKIDIIPESELRKIIEEQLMTDDSLIQESKPASQLDALYNDN
                     FEEEEDEKE"
     gene            complement(24077..24529)
                     /locus_tag="PLQ68_05095"
     CDS             complement(24077..24529)
                     /locus_tag="PLQ68_05095"
                     /inference="COORDINATES: protein motif:HMM:NF014617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HXK72058.1"
                     /translation="MSKFIAQQAFDIASEAAEKYNIEVIDSEFVKEGKDRFLRIYLYK
                     PEGITIDDCEKVHRDIDDKIDNLDIKDSYFLEVSSLGYDKNLRNKREFEIVKNTKIAI
                     NLYKQFENSKYHEGILIDRTDDFTIINKDGTEKKFANNDISKINRVFE"
     gene            complement(24652..>26485)
                     /locus_tag="PLQ68_05100"
     CDS             complement(24652..>26485)
                     /locus_tag="PLQ68_05100"
                     /inference="COORDINATES: protein motif:HMM:NF025930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72059.1"
                     /translation="KNKFESYEDICIYIANNNISSENADICNISLLVKNNEGLKNLYK
                     ILTKRNCLWNIVPKSYLEAHRYGLLIGSSDTDGELYKLLFDDADTEIIYTKALFYDYI
                     NLLSDKHGQILKDMKIVKTLTEFRNFNKFLFKCANENNIIAIASCYSNDLSKNELLTT
                     NEMLSEFSYLTTEGAKKAVIENTRQLNMEIEKIAPISINVNKEDLLSNNELLSLEDGS
                     ENFKNEIHYINKNRLSSIVLVIKKILEKLKQNDIIYQIESIMTPYIFSLILNENSLTV
                     NQTGSSDFKIHVQADKINDVFSIINDISVLHRPFFVNYQKYTAIHMLSEGFTIYDFTP
                     MKMNGNDMVTQYHSDILKNYFPTIFIQPDEDLLMIKRLKHLTDAYPTDDSLDKIAYSD
                     IHVCTEDSKFYDVISMVKPATKSEYLRCIGLIYGQGTWYDNAENLIHQKLAEIYDIIC
                     FKYENDDNKKNDEMIKESKRKIKSLITYEEAIVHGNNLLVIKWYFDNYKKQFILEYIN
                     RYRDFRRFNDEFDILPADVSISKKNSYIEQQNYKYFIEPLSVIKYIDEKVIDKIIKNR
                     PYISIDDFANRNNIDNDTLIQLKKDGYLRSLDETSQIDLSSLFE"
BASE COUNT         8023 a         4761 c         3143 g        10558 t
ORIGIN      
        1 ttatttatcg tgcttgaatt aatcattcat caaaactcaa aatcggatta tccagtggtt
       61 ctacatcaaa tttgtttgtt tcaaatattc caggcacacc tgtatcatga tagtaataat
      121 cacaagctac attttcaatt cgattattcc ttactgtaat tccagcactg cattcatcaa
      181 ggtatattcc tccaggataa ccttttcttt tagatatttt accccattta gtggttactc
      241 caagaagata tgactttttt atgtatattt ttttaactgg ttcatcttca ttgttaccat
      301 tgtcatgaat ataattatct tctataattg taccatcttg attagaaaga gtgtatatcc
      361 ctgcaccatc atgcattgat gtcatgatgt gatgtatttc atttcctttt acatgattgt
      421 tttttaatat agttggtttc gtaaaaggtt cttttactga tgaattgaat atgagatgcg
      481 caattggatc aatcgttccc catccccatc ctaaagaaat tcctgtataa gctatattat
      541 atataagatt attataaatt tttattgaat tcacatatcc agctaatatt ccacatgctc
      601 cactgtaaag tacacctata tcgaatattt tattgttttc tattaaagtc ttgtagttta
      661 tactatcttc atcatcggga tgagcatctc taacctcaaa cccgccaaca attattccac
      721 aacctgctat atcaaatatt ttagaatcta taattttact atttttacat cctcttccaa
      781 gatatatacc agttgcacct aaatgggaaa actcacattt attgaaagtt acttcagttg
      841 aataacttat gtctactgca gaaacaggta tttccctttt tgttgaatca agtccttcat
      901 ttaaattttt atatattgtt gattgcattt cacaaaatcc tttttttgaa ggatattccc
      961 atgtagtgtg tgaaaaagta atattttcaa aacatatgtt tttacaattt ttaagtatta
     1021 ctaattgctc tgcaactgga tacattacct cataaccatt tttaacttca tcatcagttg
     1081 gtatatactc aatttttcct tcattcttgt catgataaaa tgtagccttt ttaagtgcat
     1141 atttcaaatt tttcaattct gttggagcac caacacgagt aacattcttt gtttgtaata
     1201 atttaaattg attataatca agattaatta atactttatc tccatttttc tctgatgatt
     1261 ttccattagc catattttgt gtccaaccaa cattaaatac aaattcaata ttttcaggct
     1321 tttctttata taactcaata tgatctgtta agaaaccaga aaatatatct cttgaatcat
     1381 acttttcaac tgatttatga aatataaagt cctctcgctc acatctgttc caggctgatg
     1441 cttcaacctt atatgctgat gatttaaaac gtcgtatatt attaacaaac aaactatttg
     1501 gataagtgct cttgttcatt attttcttgg caccactaat tacaacattt ccgtttccta
     1561 caattttaag attgttaaca ttatctaaaa ttaatggttc atcaatttca taaaaacctt
     1621 cgtttattct tataaactct tttttttctt caatcgcttt tcttaacatt tcaatactgc
     1681 ttacttcagt atacatatgg attattctcc ttctcatgcg taatagttgt ttttattcca
     1741 tgtccaggta aaataattgt ttcatctggt aatgtgaata atttattttt tattgagtca
     1801 aataataaat cagcatcccc tgttgataaa tcagttctac ctattgattc ataaaatatt
     1861 gtatcacctg taaaaacgaa attttcagcc tttatacaaa tacatcccat tgtgtgacct
     1921 ggtgtatgta taatattaag atctacattt ccatgtttta aaaccatttt atcaataagg
     1981 ttaatgttgt tttcattaaa agttttgtgc ataccaaata atatagctcc attcttgtat
     2041 tcatctttat acaataacca atcatctgga tgagcatata tctgtgcacc agttaaagct
     2101 tttaaatcat ctccataaat gatatggtcc atatgagcat gagtataaat tattttgtca
     2161 ataacacaat tatacacctt aattttttct attatgatct tcggttcaat agatggatca
     2221 ataactgctg ctttgtcatc tgatataaca atatagcaat ttgatgatag aagctggctt
     2281 gttaatttta aaatatgcat actaatactt ttttatccta ttctctttgc tgtcaataat
     2341 tattgtaaca ggtccgtcat ttagtattcc aacatgcatt tgagcaccaa aagaacctgt
     2401 ttttacatca atactatatg tgttctttat gtatgaaata aactggttgt aatattcatc
     2461 tgctttttgc ggttttgcag cattgataaa agatggtctg tttccttttt tagtatcagc
     2521 tgctaatgta aattggctta taactaagat atctccttta atatcaatca aagataaatt
     2581 catcttatca tttgaatctg gaaatatccg catttttact attttatcag caatatatat
     2641 aatatcctct tcggtatcct caagagtcac acaaagcaga acatttaaac ctttatttat
     2701 gtttgatact ataataccat caatttttac atagctttcc ttaactcttt gaattactgc
     2761 tatcatctaa ttttctgcct ctttgatatt cttattatgt caaaaacacc tgtaatcttt
     2821 cttatttttt tgtttaattc agatatttca tctttattat gcatcataac cctaattctt
     2881 atatcagcgt atgcttcttt acttagttta gcttcaactc cattacattt tattttcaat
     2941 tcagatagta atttcattat atcggtaaca agattaggtc tgtcatatgc tttgatttga
     3001 agaactgttt catatgattc atcagaattc atattccagt atacttcaat ctttctatct
     3061 gtaaattctt tatcattaat tatattaatg cagtctgacc tgtgtactga aactcctctc
     3121 cctcttgtaa cataacctac tatttcatct ccgggtacag gattacagca cttactgatt
     3181 ttaaccatac aatttggtat atcttttaca ataatacctt cattaccttt acttgattta
     3241 taattgctgg taatttcctg gattattggg taatgatctt ctttgtgatc ttttctatat
     3301 tcttcccgta atctggaaat tatcttattt gatgttatac cgccgtaacc tatcgcgcac
     3361 ataatatctt caagctcttg aaaattatat ctttccagaa ctttttcgta atattgtggt
     3421 tttaataaat ccacaggact aaatccctgt cttttaattt ccttatttaa ttgatctttt
     3481 cccttttcaa tattctcttc tttacgttct tttctaaagt actgtttgat tttggttctt
     3541 gcttgacttg tctttacaag cttaagccaa tctcgactcg gaccttttcc tgtattagaa
     3601 gtcaaaattt caacaatatc accggtttga agggtataag ttactggaac catcttacct
     3661 ccaacttttg cgccaatcat tttattacct actgctgaat gaacatgata agcaaaatca
     3721 attggatttg acccatacgg aagattaata acatcacctt ttggagtaaa tacgaaaacc
     3781 tccatatcaa ataaatcatt tttcagattt gaatagaatt catcctcatc ttttgtttct
     3841 ttttgccatt ccatccactg agaaatccac tctaatgatg aggattgatc tgcattatct
     3901 atattctgct tatatttcca gtgtgctgca ataccgaatt cagcaactct atgcatatcc
     3961 catgtacgaa tctgaacttc aaaaggttta ccattcctat ttataagagt tgtatgcaat
     4021 gactgataca tatttttctt aggcatagct atataatctt taaatcttcc tggtataggt
     4081 ttataattct catgaagcat cccaagtgca aagtaacaat ctttaatatt gtttacaatt
     4141 attcttaaag caaaaatatc atagatctct tctagtgttt tattttgatt aaccattttt
     4201 ctataaatac tatataaatg cttaactctt ccttcaacac tcccttcaat attattgttt
     4261 ttcaggtcta taaataactc atttcttata ttatcgatca attcataacg ttctttctta
     4321 ttcttctcca tcttttcaga aagatctgca taagcttcag gataaagatg cataaaagag
     4381 agatcttcca gttcaatcct aaatgatgac ataccaagcc tatgagcaat aggagcatat
     4441 atatccattg tctcttttga tttttcaaac tgacttttct tggttttata ctctagtgtc
     4501 ctcatattgt ggagtctgtc tgcaagttta actattatta ctctaatgtc cttgctcata
     4561 gccatgaaca tttttcttat attttcaact tgttgttctt tctgagtctg taatgatata
     4621 ttagttagtt tgctaacacc ttctactatt aatgaaactt cacttccaaa catattttta
     4681 atgtcattag cagtgtattt tgtatcttca attacatcat gaagtaatgc agctgctatt
     4741 gattttgaat ttagctctag agttgctaca attcttgcta cttcaatgca atgtataata
     4801 tatggttcgc cagaagctct tttttgatca atatgtcctt caagagcaat attatatgct
     4861 ctatttatca aatcgaaatt gctattagga ttatatagct taatctcgtc tattaacttt
     4921 gcatataata attcataatt ctccactttt aagtcacctt tatgtatgtt ttcgatttca
     4981 ttaaatcttt tttttcacta ttgtttagct ttgtaacaaa aatcttttga aaatttatat
     5041 tgttatattc aataatacct aattctttca aaatatcaag acatataaga aaagtagaca
     5101 cgtttgtcaa ttcgtttaag ttaaattcac aaggtactga tttatttatt attgtattat
     5161 ataaatcagc tatattattc ctgtctattg aaattgaagt tgttgtgtat tttattagtt
     5221 catttattaa attctgctta atatcagaaa atctaatatc aacaacttca caattaatga
     5281 attttcgaat gctaagagac tttgaatttg aatctttaac tgtattacat gataatttaa
     5341 aaataatatc gtatttctgg tttggaatta gtaatttttc ataagcttct cctctaaata
     5401 gaattgtgct tattagtttt tggtttttag taaactttat atgcaaatga tctttattct
     5461 tccctactaa cttatgttca taaacttcta tctctttaag cataagcaaa cttaattcgt
     5521 tttcacaacc aaaaggttcc attaaagaca ttaattcagc atttttaagt gtcagatcat
     5581 ttatatcaat aatgaagtca ggacatattg ttttattatc ttttacttca aactttatgt
     5641 catctgcata ttgatttatc atttttgcga attctttaat tttttcatta ttgcttgaat
     5701 tatctttttt tattgttaaa ccagctgcca ttttatgtcc gccgtatgaa gatagaaaat
     5761 ctttacaata atcgattgct ttaataatat taaaaccacc aactgatcga gctgatcctt
     5821 ttgctgagcc atcctctaat atttccatta atatacatgg tttgttatat ttttcaataa
     5881 ttgatgatgc gacaattcca agtactcccc tgtcccattt ttcatttgac agtactaata
     5941 ttttgcaatt tttaatttca ctattttcat caatctctct aaaaacctgt tctgtaattt
     6001 ctctttgcaa tacttgtctt gattcattta gttcaattag atttagtgaa agcatatcac
     6061 attcgaataa atcatcagta ataagtaaac gtattgagtg ttcagcacta tccattctgc
     6121 ccgatgcatt aagttttgga attatgttaa atagtaattg agatgagttt attttatcta
     6181 ttccatttgg ataagatttt gatattaaac tccttatacc tatattttca gattttgata
     6241 acaaatccat acctattttc gctattactc gattttcttc aataagtggc accatatctg
     6301 caatcgttcc taaggctgct aaatcaatat atttggcata ttcatatcct tgacctaatg
     6361 ttatacatat tgcatgaatt agtttaaatg ctatgcctgc accgcatagg tgtttaaagg
     6421 gatataagca attattaagt ttagatgtaa taatacaata agcatcaggt aattcatcca
     6481 atggagtatg atgatcagtt ataatacagt ctatccccaa atcattcata tatgaaactt
     6541 cgtctacagc tgttattcca caatctacag ttattacaag gcctatttct tcatttaata
     6601 tttcttcaag agtatctatt gataatccat aaccatcttt tttcctgtct ggtataaaat
     6661 aagttatatt agctgtattt tgtaaaagat atttataaat tatagcagtt gcagttattc
     6721 catcagtatc ataatcccca tatatcagta taccttcttt attctttaca gcttcaagta
     6781 tcctgttaac agctttttcc atgtattcaa aaagaaatgg attatgcata tgctccagtt
     6841 ttggatatag atatttttct atgtctttga tatttcgtga atataatatt ttttcagcta
     6901 ttgtacaatt cgtaatatca gctgtatcgt caatattcca ttcattattg aaaatcatct
     6961 gtattcaacc atcccaacag gtgcaattcc gtctataaaa gatgatgctg aactatagtc
     7021 attcttaatt acgagtttcc cttcactatc aatatacatc cagcctgaat tctcataata
     7081 gtctgttttg acagcagcaa gaccttcact aaaagaataa gccatataat attttggttt
     7141 tataatcatt tcattatctt tattaatata tccgtataaa gcatctttaa acgattctcc
     7201 ttcaagtaca ggagctagtc cttctttaaa tgttgaagta ttataatact gaagtggtat
     7261 aactatattt ccttttttat caatataacc tgcatcattt gtttcctggt ctaaactaag
     7321 tttgactgat gctaatccat cactaaataa acctgcgtat gaaaactgag gttctattac
     7381 aacctctcct tgcatattta tatacccgca taaaccagca acacctgaac ctgtttgaaa
     7441 tttagctaat ccctcagaaa aagatgctgc tgcttgaaat tgtggttcta ttacatattc
     7501 tcctgtatta tcaatatatc cccaaaggtt atatgttgca tctctttttg aagactgaac
     7561 acaggcaaga ccttctgaaa aaggcagagc ataattgaat tctttatcaa aaacaatatt
     7621 attatcttta tcaataaata cccatagtga ttcatcatca tatagtttaa caggtgcgta
     7681 accttcactg aacatgcttg catccttata ctgcggttca attactactt ctcctgttct
     7741 atcacaaaat ccccatttgt agtattcatt gtacccttgt cttattggat ataagccttc
     7801 tttacctatt ttaatacctg taaatgttgg ttcaacaata taatttcctt ctgtatcaat
     7861 taatccatac agataaactg gaaattttac aatactgcaa ctgctaagta ttagtaatga
     7921 tattattaat aaaaataaga caattgcaaa tttacttttt ctcattattc cttttcctta
     7981 agatatatat tcagctcatc aagttgcttt atatcaactg tatttggagc atctgttaat
     8041 ggacaagcac cactttgtat ttttgggaaa gctattacat cccttatatt ttcagttttt
     8101 gtaatactca ttacaagtct gtctaaacca taagctattc cgccatgagg aggagctccg
     8161 tactttaagg catttaataa aaagccaaaa gattcttcag ctttttcttt cgtaaaccct
     8221 aaaatttcta acatttcttc ttgaagttta taatcgtgaa tccttatact tcctccgcca
     8281 atctcttcgc cattcaaaac aatatcatat gctttagctc tgactttatc tggttgtgtt
     8341 cgcaaaaatt catagtcttc ttccataaat gatgtaaatg gatgatgttt tgcgtagtac
     8401 ctgttatctt cttcactaaa ctccagcaaa ggaaattctg ttacccataa aaactcatat
     8461 ttgctgttat cagtaagatt aagtctttta gcaatctcaa ctcttaatgc tcctaatgaa
     8521 tcaaatacta cttgattttt atctgcaata aataaaagca aatcatttgc ctttgcatca
     8581 aagattttta gtatctcagt taattgctct tcattaaaaa atttagtaat agatcctgaa
     8641 atttctaatg tatctgaaac cttaagccat gcaagtcctt ttgcaccaaa atctttaacg
     8701 aaatcagtaa gtttgtctat ttcctttctg gaaaatcctc cacatccttt tgcattaatt
     8761 cctctaacag atccgttttt acatgcatca gagaaaactt tgaaatcaca attttcaact
     8821 tttgaagata tatccttcaa ttccattgaa aatcttaagt ctggtttatc acttccaaat
     8881 ctcgacatag cttcttcata tgtcattctt ttaaatggtg ttataagatc aatattaaga
     8941 gtgtttttaa ataattcttt aataaaagct tcattgattt ctataacatc atcaatatca
     9001 acaaaactca tttcaaggtc aatctgagta aactcaggtt gtctgtttgc tcttaaatcc
     9061 tcatctctaa aacattttac tatttgataa tatctatcaa atccagacat cataagtaat
     9121 tgctttaata actgcggtga ttgaggtaaa gcataaaatt tattttgatt taccctgctt
     9181 ggaacaagat aatctcttgc accttcaggt gtacttctcg tcaaaacagg tgtttcaact
     9241 tcaataaatc cattattagc aaagaaatct cttgctattt tggaaatatt acttctcatg
     9301 aataaatatt gctgctgttt tgattttctt aaatcaaggt atctgtattt aagtctaagc
     9361 agttcattag ttgaagtatt atcggccata tcaataggtg gagttgaact ttcagatagt
     9421 attcgtaatt ctttaacttc aatttcaata tcacctgtag gaatttgaga atttttatcc
     9481 tctctttctt ttatctctcc ttttatagca attacatatt cactctttaa aagagttgct
     9541 ttttcaaata aatcctgatt gttttcatgc ctgaatacta tctgcataat acctgttcgg
     9601 tctcttaaat cagcaaatat taaacttccg agattccgtg atttgtttac ccatcccata
     9661 agtactactt ctttaccaat attactgata ttaaattgtg tgcagtatcc acttctttta
     9721 aaacctttaa ttgtttccat atttaacctc aaatcttatt ctcaattatt cttcttgtta
     9781 tagaatctac acttacttct ttagtttcct gtgtcaacat atttttgata atggctttat
     9841 ttgaatcaat ttcattatct ccaataacaa cactgtacaa ggcattcttt ttgtctgcaa
     9901 atttcatttg tgatttcaga cttctatcag ttacatcaat ttcacaataa atatctttat
     9961 ttcgtaaatc attagccatt tttcttactg ctttatatgc tttctcacca atgaaagcaa
    10021 agaatattac tggttttttt tcgtctggaa gtttgaatcc tgatttttcc ataacatcaa
    10081 ttaatctttc aagacccatt gcaaaaccaa ttccaggagt atcgttgcct cctagttcag
    10141 ataccaatcc atcatatctt ccgccagcac atattgtatt ttgagctcca ataccttctg
    10201 caataaattc aaaaactgtt cttacataat agtcaagtcc tcttacaatt cttttattta
    10261 ctatatatgg aacattcatt tccttcaatc ctttttcaac aagatcaaaa tgatttttac
    10321 agtcatcaca tagattatca agtaacattg gtgcatcttt tgatataatt gaacatcttt
    10381 cgtttttaca atcaagtagt ctcattggat ttttttcata tctcttttta caatcatcac
    10441 acatatttga aatcttatca gagaaatagt ctttcagcat ttgattgtat ttgtctctac
    10501 atgatttaca tcctatgcta ttaatattca gctgaacatt tgaaattgat aattttgcaa
    10561 ataaagcatt aagcattacc attatttcaa tatctgcatt tgaagaatca gaaccgagaa
    10621 cttcagctcc taattgatta aattctctat atcttccttt ttggacattc tcatatctat
    10681 atgcagtaat atcataatat aacttaatgg gttgtggttg tgatgacata ccattttcaa
    10741 tataacttct tactactgaa gcagttcctt ctggtcgaag agtaattgat cttccaccct
    10801 tatcaagaaa ggtatacatc tctttttgca ctatatctgt tgtatctcct acacctcgtt
    10861 gaaatagctc tgtatattca aacacaggaa atcttatctg ttggtaccca tagttctcaa
    10921 gtaattttga aaactctgtt tgtacatatt tatatttttc aatttcacca ggaagtatat
    10981 ctttagttcc ttttggaata gatatcttca tttcgtcctc ctttatcaaa aaagcctttt
    11041 acataaacta taaaagacga tttaaatcat gacacatgct aaaatttaag tttattctta
    11101 aggcaagcat acatccttta ctttgaatta tttaatacta aaagtaacat tttgtcaagt
    11161 atagcctatt cagatattga tataatctgt tgttttatgt cttcaggttt agttatgtaa
    11221 aaactctttt tattaggctt accttcatca tcaattaatc cagtatctac cattttctcc
    11281 tctcctttta aagtataata tgatatatta tcagtattta gcgataaaga aaaggttatt
    11341 aatgaagtaa gtttgtcttt actgatgtta gttcttatat tgttttttgc aacatcaatt
    11401 actgaagaaa tatttgataa tgttaaagct ttttttaaaa ttgcatttat cattttatgc
    11461 tgtctttcta ttctggcaat atctgatata tattcatatc catcaccttg tctgaatctt
    11521 aagaatttaa ctacattctc accatcgagc acttgatgtc cttcatcaat ttctataaac
    11581 aactcatgtc cttcagtata atcataataa tgcatatcaa aaggaacatc atattcaatt
    11641 cctcccatag catcaacaat tctctctata gtatcatatg atactcttac atagtgatcc
    11701 atatcaatat ttaaaagttt ctcacaggct cctaaaagat tcatggatga agttgagtca
    11761 tcagttttta tccaaatatc atctttatag ttattatagt atgaattaat tttccaatgt
    11821 tcgggattat tcctatcaga atatctcaaa tatgtatctc ttggtatact taaaatattt
    11881 actgtattat ctgttttatt tataacagct atccaaatag tatctgcaag attatattct
    11941 gctaatccaa aaagcaaaat atattctata tcatccacag gtttatactt gacgtcttct
    12001 gtcggttctt ctgtctgtgt tggttgttca gattctgttt ctaatggagg caatgtaatt
    12061 atttcaatat tttgacttga tgttgcatca ggtgtttcat aaatagagtt atgcataata
    12121 tcataaaata tcaataatga tattattata catagaataa gcaatgcagc aactgatatg
    12181 attataatac ctttattact taattttctt gtttttggtt tattattctt tttaatacta
    12241 ttatttctaa tttcactcat atcaatatcc tgattttcct aataatttaa acatattttt
    12301 tttgtatgct tcaacccctg gctggtcgaa gggatttata cccaaaacat atccgcttat
    12361 tccacatgct ttttcataaa aatacaccaa agaaccataa ttatatgcat tcatcttttc
    12421 aatttcaagt aaaataactg gaacgtgacc atttacatgt gcaagtttag ttccctgcat
    12481 agctttttca ttcacatatt gtaaggattt tcctttaata taatctaaat catctaagtt
    12541 atcagatgat tcaggaatcg agatttttga catatcatca cttataaata gaacagtttc
    12601 aaaaatattc cttattcctt cttgaatata ttgacccatt gagtgcagat cggttgtatt
    12661 atctacacct gacgggaata tacctttatt ctctttccct tcagattctc caaaaagctg
    12721 tttccaccat tctgtaagat aatgaagtga aggttcataa tttactgtaa tttcaatagt
    12781 ttttccaatt gaatacatga gatttctagc agctgcatat tgtaaacact cttcttcatg
    12841 attatccatt gagtattttg cacctttaag catcatatca atatcaattc ctgcaacagc
    12901 cataggcagt agtccacatg gagtcaatac actgtatctc cccccaatat catctggaat
    12961 tacaaaagtc ttataaccca aatcatttgc tatctggcgt aaacttccct tttctttatc
    13021 agttgtaacg aatattctgt cctttgcttt atctttatac tttttctcaa tttcgttttt
    13081 aaatatacga aatgcaattg caggttctgt tgttgttcct gatttactta taacattaat
    13141 tgagtaatct ttgttactaa tatatttcaa tattgattca atatatttac cagaaatgtt
    13201 attccccaca taaagaatct ctgttccttc aatatttttt gtatctgtta atgcttctat
    13261 tgcagctctg gatccaagat atgatcctcc aatgccaata actaacaaaa cttctgattg
    13321 atttttaatt ttttctgctg tttgttttat atcttttatc tcatctgttg ttatttggtt
    13381 tggtaaattc agccagccta aataatctga tcctctacct tttttgcttt ttagcaattc
    13441 attagctttt tttgcatttt ccaacatttt ataatagtca tcattttttg caaaactaat
    13501 acaatcatca agaaatattt tcattatcat cctctacttt ttcaagtttt attacagata
    13561 cctcataggc ggtttttgtt attatattac cgtagtgatc ttttttttca tattcacgac
    13621 tttgaaatct tccccaaatt ttcagatgtg taccaatagc catattctca caataacgcg
    13681 catttcttcc ccagcatata caaggaatat aatcactttt tttataagct ctatttacag
    13741 caactaagag atcacatatt tctcgaccaa atggtgtaac tctgaaattc ggatctttgc
    13801 aaataaatga atcaaaaaat acatcattaa aattatctat atccttaaag ctttctatta
    13861 tttttgcttc tcttgcaaat atattaacaa caagtctttt tttgtctgct ttatcaatat
    13921 tattatatga acgcacttgt ccatgtactt cgagcattac tttaggttta agtaaatcaa
    13981 tatccagtaa atgatcagat ataagaatag gaaccatatc agtttgtttg cttaaacgtt
    14041 gtacttctaa tataacgtta taaaatgatt caccatatat ctggtgattg aatacagggg
    14101 ttttagcaat aactccagca acatatatct tattgttctc taaaagctcg atctcattta
    14161 tcatttgcat accaggcttt cttttgtatt acataaaaat cgaagtcaat agactttgca
    14221 cttattatat tattactttt aaaaaaaata aaggtaaata tgtaaattca tattactaat
    14281 atctaatttc atgtaatttt gtctaaaata tctatattat gcaaatttaa taaatagcaa
    14341 ctagtaaaag ttaaagtatt ttttccataa taataaggaa tatttgataa ttcacaaact
    14401 ttacttaccc ataaaccata tgattccatt gaaccaattg ccttatttgg aaatctgcat
    14461 ggttcatctg gatatgaaca tttattgcat attgaacaag tgccggcact cataggtaat
    14521 atatcagaat aattactttt aagatcatca actaatttca taaaattgtt tttatgttta
    14581 atttcagtgt tatgaattgt atcatagtca aattcatctt ccattaaagc tgttgtttgg
    14641 acaatcatac cgaaattata atttttcact cttaatgcaa cattttccaa tgtgcctatt
    14701 gctggtggac acatccagtt tttattgtaa cttttacata aatcagaatt gcacatttgt
    14761 ctgacttcag gcataaatac taatgaatct aaattaagtt cacctgaatg agtaaatcca
    14821 aaatcttcag catgcttcat caatgtttta atcacttttt tcattattcc tcctaaaaaa
    14881 aatcgattat catgggataa tcgattatat tggtcggagt gacaagattc gaacttgcga
    14941 ccccttgaac cccattcaag tactctacca aactgagcca caccccgact tgtctaatta
    15001 taactttaac ttattttttg tcaacagatt cagtctttga ttcatcagtt gaaatatcat
    15061 cagttatagt ctttttattt atttctgaat tttctttatc tgaattactt gattggcctg
    15121 aagtatttgc aactggttga tcttcaggga caaatgtgtc tctatcaaca agtaattctt
    15181 tatagtctga ctgaccaaca aggatatctt cttcgtcaat tagtgatttt aaaaataatt
    15241 ttttatcttt tgatctaagt attgaaatta gtattagcat agaaccaaag aatatcgcac
    15301 ttataagcgc tataagcctt gaaagcttta tatcagtatt aaatagatac aaggggtcta
    15361 ttcttattcc ctcgataaaa aacctgtata atccatatga agctgcatat atgcataatg
    15421 aacgaccatt gaagtatttg ccatttcttg caactaaaat aagtacaagg aataatgcaa
    15481 gatttaatat tgactcgtag aagaatgttg gatgaacacc aatatagtta aatgtcgtct
    15541 cgttaacatt tgctattaat gaggtatcaa caagtcctaa ttgaagaagc tcctcttcaa
    15601 ttgatccagc tacattgcct ccgattttca tgcttgtcat tgctaaacta ctcaaaatac
    15661 tgcttggttt cacaggtaca ccatatgctt ctttattaat gaagtttccc catcttccta
    15721 ttgcttgagc taatgcaatg tatgggacta caagatcagc aagtttcaaa aaattaattt
    15781 tcttaatctt gcaatatatt gctgttgcag cgaaacctcc aattaatgct ccaaatattg
    15841 acattcctcc atcacgcaca tttaaaacat caccaaaagt ccactcttgg tctaatcttc
    15901 caagaacgta caataatcta gctgaaataa cacctacaat aataccaatt aaacaaatat
    15961 tgagtaggtc atcttcattt aaaccatatt tttttgctgc cattaaaact aaaacaacag
    16021 caagacaaac agcaattgct ataagtaatc cataccaata aacaggaaac ctaccaatat
    16081 taaatgcaac tctgtttact gaaagttcta agcctccgaa taaatttgga aatgacacag
    16141 tacttctcat ttatccacct cttttctgaa tttgctacat aataagtatg cacaataaag
    16201 tatactactc aagttgtttt ttaacaagca aaatatcgtt ttctattgct tttcttaact
    16261 gatcaagact atcaaatttt tgttcatttc taatctttct tttaaattgg ataacaattt
    16321 ctttaccata caaatttaag ctagagtacc ctattacatg agtttcacat gaaattctat
    16381 ctgttaaata atcattgatt gtgggattag aaccaatatt tgtaatacta tcgaataatt
    16441 cattatcaat agatgatttt gtcaaatata ctccgctacc aggaagtttt atattcttac
    16501 ttatatcaat atttgcagtt ggaaaaccaa tcttgcttcc aagtccccga ccatgtacaa
    16561 ctgttccttc tattgaatat cttcttccta aaagcttatt tgctttttca atatcacctt
    16621 catcaagcaa atttcttatt cgtgaagcgc ttactggttc attgtaaaaa tcaactttgt
    16681 cgacaatagt aacatcagtt ttaaatttaa cacaaatttc ttttaagtat tgagtatcgc
    16741 cttttgcccc cttaccgaat ctataatcaa aaccagcaac tatagatgca gctagtaatt
    16801 tatctataag aatttttata atgaaataat caggctctaa tgttgaaaat ttaacatcaa
    16861 actcttgaat ataaacatag tcaacaccta gttgttcaag tatttttatt ttttgctcaa
    16921 gtgttgttaa taacttagga gcagaatcat ttaaaacaga aattggatgt ttattaaata
    16981 gtaatactaa agatgtcaaa ctatttttct tagctatttc aactgctgtt ttaataacct
    17041 tttgatgtgc aatatgaata ccatcaaaat taccaagtgc gattacatta tattttgtaa
    17101 atctataacc ttcattactg gttattacat tcatacaaca tgacctttcc ttttaaaata
    17161 ccaaaatcat ccactttccc tatacctatt attgtttttt gttcatcttt tattatatat
    17221 tcatttgatt tataatttgt ttgaattttt attccattta aatatttttt taaatcctcg
    17281 tctttaataa tcaatgtatc aagaaatata gcatcttcta taggagtaaa tatatcattc
    17341 aaccttttat tttcatgaaa tttaattatt tcatttattg tataactatt ttcagtttta
    17401 aaattaccga ctgattttct agttagattt gacattgttc cgccagattc aagaatttca
    17461 cctatatcat tacaaagact tcttatgtat gttccctttg aacaatatat tgtaaatata
    17521 acctttttaa ctttaatttc ttgataaaag tcatcagtta tggagtcaag cgaaatatca
    17581 tatatattaa tcactgagtc ttttatatca acttttttat cttgcaatgc atattgatat
    17641 agtttttttc catttacttt ttttgcagaa taaatgggag gtttttgatt ttgtatacct
    17701 ttaaaagaaa gaatagtctt tctaatcatt tcatctgtaa tatcagatat atcataaaca
    17761 tcagttattt ttccagttat atcataactg tcagtagaaa acccaagaag taattctgcc
    17821 tgatatgttt ttgtttggct ttggatatag tcagcaagtc tagtagcttt gccaatacaa
    17881 acaaccaata caccttgtgc taaaggatca agagtacctg tatgtccgat tttttgatta
    17941 ataaaaatac ttttaagcat ttttatctgc ttaaaagatg aaatgtcttt ttctttgttt
    18001 aaattaatta ttgcatacat tcaaataatt cattcaggtt cataatgata ttttcgtaaa
    18061 cttcatttat atttttttta cttattaatc ctgctgccct ggcatgcccc ccaccaccat
    18121 taagttgtgc tattactgaa acatcaactt tatcggtgtt tgatcttaaa cttcctttta
    18181 cttcttcttt atttggtctt tctcttaaga aaacacctac ttcaacacct tcaatatttt
    18241 taacaatgtt aacaagtcct tcaaaatctt catcattaac atcaagattc aagtattcct
    18301 ctgtcctcaa tgttgatact gcgactttgt tattgcaatg taattttatg ttttgaattg
    18361 ttttttgaaa aagatagagt tttgttaaat ttgttctttc aaaaatccat tcagaaatct
    18421 tttcaatatt aattccgtaa ttcattaact ctgatgaaat cttaaaacat tttttatttg
    18481 tgttctgata tctgaatccg ccagtatctg ttgatatagc tacatatatt gcttgagcta
    18541 tatcatgatc tatttgtgct tttagtaaag aaagtaaatc ataaataatc tcaccagatg
    18601 atgatacatc ttcgtttaca tgattatact ttgcataacc attgtttgtt ttatgatggt
    18661 ctatattata tgaaagttta ttttcaaaaa ttgcttttct cttacctaat ctgtttatat
    18721 ctccagtatc aattgcaata tatacatcat aattattata ttccgattct tgagaataaa
    18781 ttcttatatc agccgaattc tgcataaaag agtatatgta tggaattttt tcttccataa
    18841 gaacaacagc tttttttcca agagatacta gatatcttgc cattgcgata caacatccta
    18901 ctgcatctcc gtctgccata acatgaggaa gaatcagaaa gtttttattt gattctatat
    18961 ctttattcag ttggtttgct atctttgtta atttcatcta ttaatttact aattcttata
    19021 ccttgctcta tatagtcatt atacttaaac cttaattgtg gaatatatct taacatcatt
    19081 ctctgtccca acaattttct tatataacca gctgaattct gtattgctgt aaaactatct
    19141 ttttttgatt tttcgtcacc aagcatgctt acatgtatat cagcatatga tagatcattc
    19201 gtgatgttta cattaacaat tgatacaata gcggtaagcc tcggatcctt aatttcttca
    19261 gcgagtattg aagagatttc tcttttaaat tcgctattta gacgttctac tcttttttgt
    19321 ttcattcttt aatgacctcg accttatagc attcaattat atctccaacc tttaagtcat
    19381 tatacttctc tatagacata ccgcattcgt aacctgctgc aacttcctta acatcatcct
    19441 tgaatctctt aagagaactt aatgctccat caaatatcgt gattccatct cttacaagtc
    19501 taacattagt gtttcttaat attttaccat caagaaccat acagcctgca actgttccag
    19561 cacctgtaat tttgaatgta tttctaactt cagcatgtcc ataaacaact tcttttgttt
    19621 ttgggtctaa catacctgcc atagcatttt tcaaatcttc tatcgcatca tatattacac
    19681 tataaagcct aatatcaact tctgctgatt tagcagcttc tgtaacattc cctaatggtc
    19741 ttacattaaa gccaattatt atagaatttg ttacttcagc taacgataca tctgtttccg
    19801 atattgttcc aactgcacca tgtataatat gcaccttaac ttcatctgtc gacagtttct
    19861 taaatgaatc agaaattgct tctacagaac cttgaacatc agctttaatg attatattta
    19921 aatccttaac attaccagct tgaatctgat catataaatc atcaagtgtt gttaacttat
    19981 gagatgtttt aataacttgt ctctcatgtt ttcttctttc agctaaagat tttgcggttt
    20041 tttcatcatc aacagcataa aacatctctc ctgcatcagg tggtgaatct aatcccaata
    20101 tttctacagg cattgatggt cctgcacctt ctatattttt acctctgtca tcatacaacg
    20161 cttttatatg tcctactgaa gttccggaaa taatgtaatc agttttcttt aagcttcctc
    20221 tttgtatcaa taaagtagta acagttcctg ctttttccat tcttgattct aaaacaatgc
    20281 cttttgcttg tctgtttgga ttagctttta attcaagaat atctgctgat agcagtattg
    20341 tatcaagaag gtcatcaata ccctgtccgg tttttgcaga tacaggaata cattcggtat
    20401 ctccaccata ttttgttgct acaagattat gttccataag ctgttgcata actctgtctg
    20461 gatttgctgc aggtaagtca attttattta ttgcaataat aattgtaaca ccagcagctt
    20521 ttgcatgatt tatagcttca actgtctgtg gcataatacc atcatcagca gcaacagtga
    20581 ttattgctat atcagttaca tcagctcctc tttttctcat agctgtaaaa gcttcatgtc
    20641 caggagtatc taaaaatgta atatatcttt catttatttt taccctatat gcacctatat
    20701 gctgagtaat ttttccagct tctttttcgg caacattgga attcttaata taatccagta
    20761 aagaagtctt gccatggtca acatgaccca taacaacgac aacagggggt ctttcttcaa
    20821 ggtcatcaat tttatcttct gcttcgtcat cgaataaaat atcttcttca gttttaatgc
    20881 tttccatttc gcatgttata ccaaattcat ccgcgattaa agcagctgag tcaaaatcaa
    20941 tattctcatt tatactcatt atgtccgaac ccataagtag taatttagat aaaacatcag
    21001 aactggtttt ctttattttt tcagcaaatt ccttaacagt catttctggt ggaagtttta
    21061 cgaatttcaa tacttcaatt ttggcaggta tgttttctct tttctttctc cttctttttg
    21121 gtgcgctatc tctataaaaa gttgtgaaga tttcatcttc tgtcaaactc ttagtatcta
    21181 ttgcgctttt tggagtgatt atctctcttt tatcgtattt agactgtttt gcagtaatac
    21241 ctttactaac tgcagcttcc tttttgttat ctttatttaa tactttttct tgatcttttt
    21301 ttaagagttc ttttttatct actcttattt tatcagcttt tacccttatc tcttcgacag
    21361 atttgtcctt gtccacaggc tttttatatc cctgtctgtt tctatcagtg gattgatctt
    21421 tatctttgtt ataaccctgc gaacttggaa atgtacccgc tggacgagtt ctgtaaccct
    21481 gacttgtatt tgactgatta taaccaggtt ttctttcgcc tctgttaatt ggtttatctg
    21541 gtctatttgt tttctgcgca taacctttat ctggtctatt atctttatgc acaaatggct
    21601 tatcacttct tgtacctgga atatatgtat tattcttata ttctttatgt tctctataag
    21661 ttttttcctt tttttcctct ttagtgaact cacgttcttt tgtttgcttt tgattttcaa
    21721 taggcttttt ttccactgtt tgttgttcag gttttttttc tacagatggt tgttcagctt
    21781 ttgtttcttt aatttgagac tcaactttta ctgattctac aggttttacc tcttcatttt
    21841 ttttgtctgg gacaacaact tcaggagttt taactacaac aacttttttc tcttggggtt
    21901 tttctttctc ttttgtaggt tcaggtgcat tttcaggctc ctgcttaatg tgcttgatag
    21961 ttgactttgt ttctccgcct ttcgttgtct ttacaactac aaccattggc ttttctacag
    22021 aatcatcgct tttagactta tcaatctgct tttctggtgc ctttatgact acagttttag
    22081 tctttgttga atctggtgat gttactgtaa ctacctttgg attttgtttt gctgatgtat
    22141 ttttaatacg atatacatca tatatcatct gttcttgttc atcagtaaga actgtattta
    22201 cagtacagct tattccaagc tgatccaatt ttctcaatac ttcactggct tttttgtcta
    22261 tttctcttgc taattcgttt acttttttcc ccatttagtt ctccttggtt atcttctctc
    22321 taatacctct tgcaaagtta tagtcagtta tagctataac ggatatttct tcttttccag
    22381 taatatctcc cagttgattc ttagaaaatg tataaaaagt ttctaaactt tttttctcag
    22441 ctgctgatac taccttcttt tttgtgtttt cagtgcaatc tgatgcaata aacaataatc
    22501 taactttatc tttttttata tattcaaggc ataagtctgc acctgttaca cattttcttg
    22561 cttttatagc aagtccaatc attaataata ctctattttg ttctatttat atattcctcc
    22621 aacttattgt ataaattatc atctattgtt gctgaaaaat ttctatttaa aatcttgctt
    22681 tttcttgctt tctcaaaaca ctgaatatca tcgcagatat atgcacctct accattcatt
    22741 ttaccagtaa tatccatgtc tacactgcca tcatcttttt ggacaatgcg tatcaattcc
    22801 ttttttggtt tcattgttct acatgtcaaa cacatgcgca atggtgtttt tttactcttt
    22861 ttcatcctct tcctcttcga aattatcatt atatagagca tcaagttggc tggcaggttt
    22921 agattcttga ataagtgaat catctgtcat aagttgctct tctattatct ttctaagttc
    22981 ggactccgga ataatatcaa ttttccaatt tgtaagtttt gctgcaagtc tcgcattttg
    23041 tccttcttta ccaattgcta acgataattg attgtccggt acagttaccc tagctttaat
    23101 ttctttatcc ccattactgt tttctacttc aataacatct actctagaaa catcagcagg
    23161 acttaatgca gaagatataa actgagcagg atttgcactc cactcaataa tatcaatctt
    23221 ttctcctctt aattcctcaa ctacatttga aactcttatt cctctgtgtc cgatacaagc
    23281 tccggtagca tcaacatttg aatctgaact atatacagca attttagttc ttgctcctgg
    23341 ttcacgtgca atatctttaa tttctactat tccactctgt atttcaggaa cttccatttc
    23401 gaataatctt tttacgagat cgggatgagt tcttgaaaca aatatctttg gttctttgct
    23461 ggttttcttt acatcaaata caaatgcttt aaatttaaaa tttattccgt atctttcact
    23521 tggaacctgt tctttctttg gaagaatcgc ttcagtattg cctagatcca gataaacagt
    23581 atcattttct attcttctta cgacaccatt tactatttct tttaacttat tttcatatat
    23641 atctaatatt tttactcttt cagcgtcttt tattctttga agaataactt gctttgctgt
    23701 ttgtgcagct attcgaccaa attctctagg aagcatttct tcttcgaatt tcagacaata
    23761 acctacttca acatcagcat attctttcgc gtcttcaata agcatttcat tttcttcatc
    23821 aactatttca tcaacaatgt ttttaacctt aaaaagtttt acttcaccag tattaacatc
    23881 catagttact tcaatatttt gttcggactt tgtatgtttc ttatatgctg aagctaatgc
    23941 agcttctata gcttcgataa gggttttact atcaataccc ctttctcttt ctatctgcct
    24001 gatggcatca atcaaatctg tattttgccc taaattcgaa ctactttcct ttttcattaa
    24061 ttatatatcc tccttattat tcaaacactc ggtttatttt cgaaatatca ttattagcaa
    24121 atttcttttc agtaccatct ttattaatta tggtaaaatc gtcagtccta tcaataagaa
    24181 ttccttcatg atatttgcta ttttcaaact gcttatataa atttatggct atttttgtgt
    24241 ttttaacaat ttcaaattct cttttatttc taagattctt atcgtatcct aatgaactga
    24301 cttccaaaaa atacgaatct tttatatcaa gattatcaat tttatcatca atatctctat
    24361 gaaccttctc acaatcatca attgttatgc cctctggttt atatagataa atccttaaaa
    24421 atctgtcttt tccttctttt acaaattcgc tgtcaattac ttcaatatta tatttttctg
    24481 cagcttctga tgcaatatca aaagcttgtt gagcaatgaa tttactcatg ttactcctta
    24541 tcataaagta aagagtggct agccactctt ttgtacttta aaactttacc atagatgctt
    24601 ttttttgtca acttttttta aaatatattg aaaacaagat atttctacaa tttactcgaa
    24661 tagtgatgaa agatcaatct gtgaagtttc atctaaactc ctcaaataac catctttttt
    24721 aagttgtatt aatgtatcat tatcaatatt gtttctattc gcaaaatcat caatacttat
    24781 ataaggtctg tttttaataa ttttatcaat tactttttca tcaatatatt taattactga
    24841 taaaggctca ataaagtact tataattctg ttgttcgata taactatttt tctttgatat
    24901 tgaaacatct gctggcagaa tatcgaactc atcattaaat cttctaaaat ctcgatatct
    24961 gtttatatat tccagtataa attgtttttt gtaattatcg aaataccact taataaccaa
    25021 aagattgtta ccatgaacta ttgcttcttc atatgtaatt aaacttttaa tttttctttt
    25081 tgattcctta atcatttcat catttttttt gttatcatca ttctcgtatt taaaacagat
    25141 aatatcatat atttccgcaa gtttttgatg tataagattt tctgcattat cataccatgt
    25201 accctgacca taaattaatc ctatacatct taagtattca ctttttgttg ctggttttac
    25261 catgctgata acatcgtaaa atttggaatc ttcagtacaa acatgtatat ccgaatacgc
    25321 aattttatca agtgaatcat cagtaggata agcatcggtt aaatgtttta atctttttat
    25381 cattaacaag tcttcatcag gctgtatgaa aatagtcgga aagtaatttt ttaatatatc
    25441 tgagtgatac tgtgtaacca tgtcattgcc attcattttc attggtgtaa agtcataaat
    25501 tgtaaatcct tctgataaca tatgaatagc tgtatacttt tgataattta caaagaaggg
    25561 tctatgcaat acagatatat catttattat ggaaaataca tcatttattt tatctgcttg
    25621 tacatgaatt ttaaaatcgc tacttcctgt ctgattaact gtaaggctgt tctcattaag
    25681 aattaaacta aatatataag gtgtcattat cgattcaatc tgataaataa tatcgttctg
    25741 ttttagtttt tcaagtattt ttttaattac taaaacaata cttgaaagtc tatttttatt
    25801 tatgtagtga atttcatttt taaaattttc agaaccatct tctaaactca acaattcatt
    25861 gttacttaac aaatcttcct tgtttacatt aatagaaatt ggtgctattt tttcaatttc
    25921 catatttaac tgtctagtat tctcaataac agcttttttt gcaccttcag tagttaaata
    25981 ggaaaactca gatagcattt catttgttgt caataattca ttttttgaaa ggtcattgga
    26041 atagcatgaa gcaatagcaa taatattatt ctcgtttgca catttgaaaa gaaatttatt
    26101 aaaatttcta aattcagtta aggttttaac tattttcata tctttaagta tttgtccatg
    26161 tttatctgaa agaagattta tgtaatcata aaacaaagct tttgtatata ttatctcagt
    26221 atcagcatca tcaaaaagta atttatacaa ttctccatct gtatctgaag atcctattaa
    26281 aagtccgtat ctgtgagctt ctaaataact tttgggtaca atattccaca aacaatttcg
    26341 ctttgttaaa attttatata aattcttgag accttcatta tttttaacta ataatgaaat
    26401 attgcaaata tccgcatttt cggaactaat attattattt gcaatataaa tacagatatc
    26461 ttcatatgat tcaaatttgt ttttc
//
LOCUS       DAOSXM010000025        25010 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2111
            NODE_1277_length_25010_cov_10.955720, whole genome shotgun
            sequence.
ACCESSION   DAOSXM010000025 DAOSXM010000000
VERSION     DAOSXM010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864178
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 25010)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25010)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:24:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,261
            CDSs (total)                      :: 2,229
            Genes (coding)                    :: 2,218
            CDSs (with protein)               :: 2,218
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 3 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25010
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1277_length_25010_cov_10.955720"
                     /isolate="AMR_MDS_2111"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..378
                     /locus_tag="PLQ68_05105"
     CDS             <1..378
                     /locus_tag="PLQ68_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010502272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine synthase F"
                     /protein_id="HXK72060.1"
                     /translation="ILMDKNKYEKEYIVTVDKEITSAFIEGMSKGVRIFNPVKNNYTR
                     TNRCKIFKIDSRTFKIVLTQGLNRQIRRMCSHFGYEVIKLKRIRIMDISLKNLPVGKW
                     RNLTNEEMKSLLAAKKADKKNDK"
     gene            423..1295
                     /locus_tag="PLQ68_05110"
     CDS             423..1295
                     /locus_tag="PLQ68_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016112807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HXK72061.1"
                     /translation="MKTFKKVYIEITNICNMKCDFCPKTNRPLEFMNTETFSKILYQI
                     KPHTNYIYLHVKGEPLLHPELDKILDISYEKGFHVNITTNGTLISSVKDMLMTKPAVR
                     QINFSLHSIDGNKMFKNELAYIENILSFTKESIEKTEMIVSFRLWNLNEDNATTLKVR
                     KNNNILEAIETFFKLQYSIEETKGIKSGIKIAERVYINQAQQFKWPDINEKEINECGF
                     CYGLRNQAAILVDGTVVPCCLDNDGVINLGNIHNKSFSEIIESERANNIVNGFTKRHV
                     VEELCRKCGYMRRF"
     gene            1418..1729
                     /locus_tag="PLQ68_05115"
     CDS             1418..1729
                     /locus_tag="PLQ68_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair di-iron protein, ric"
                     /protein_id="HXK72062.1"
                     /translation="MAKEKIFNKEEQQIIKTLRQFVPVVDRVHGSTHPEFHEVKRLFE
                     AIDEKVKSNASEKYQLTEEFAKLSEITSNYTVPNDVCESYEAVYVMLAELNEAYNEIN
                     K"
     assembly_gap    1919..2018
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2019..3380
                     /locus_tag="PLQ68_05120"
     CDS             <2019..3380
                     /locus_tag="PLQ68_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HXK72063.1"
                     /translation="GYINEASITGESFPVSKAKGSLVYAGTILENGTLQITADRVGED
                     TTFGKIIELVEEAQDSKSEAERFIDKFSKYYTPAVLVLALFVWIISKNLELAITILVL
                     GCPGALVIGVPVSNVAGIGNGARHGVLLKGSEVVQDFSRTNTIVFDKTGTLTIGRPKV
                     VDIKTYTKEIETALSYLVSVENESDHLLAKAVIEYANNPKYYPVENTDVIKGKGIVAD
                     VDGHRVAVGNMALMEHEKVNISKEMYEDISRFEQNGDSLVLTSVDGELNILMGIRDQL
                     RPDVKNHLQKLKALRVKNLIVLSGDNQSTVDKVAEELGLTEAYGNMLPQDKSQFIRNL
                     QENGHKVAFVGDGVNDSPSLALADIGIAMGGGTDVAIETSDVVLMNSDFSRLPHALGL
                     AKATARNMIQNIVIAVGVVLFLLANVFFSEWMNMSIGMLVHEASIFVVILNGMRLLGY
                     KSR"
     gene            3435..3665
                     /locus_tag="PLQ68_05125"
     CDS             3435..3665
                     /locus_tag="PLQ68_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003106687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HXK72064.1"
                     /translation="MKKATIQLETLTCPSCALKIEKAVKSLNGVDKDSVNVAFMSSKV
                     KLEFDDDKLKVDEIEKAITSLGYEVKKTQVKA"
     gene            3894..5777
                     /locus_tag="PLQ68_05130"
     CDS             3894..5777
                     /locus_tag="PLQ68_05130"
                     /inference="COORDINATES: protein motif:HMM:NF019552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HXK72065.1"
                     /translation="MLSELLNKNIIQKTEPGMFSLFPVYEDRNAWESIDHKLKTHIIN
                     EAEGLIRHTWPRICDEDYEDYHKTGSRKKYDEKFKNLYDSLTKIVLAECIEGKGRFIR
                     DIAAGITTRLNETAWINPAHLKHYGEDIIPVPDGKYLDLRCCSTAKQLAVIYYLLGKK
                     LAEYEPLLLDRMEKEIKIRVLDRYIDTDQWWTNLSEGFVINNWNTHCNKCLIITAMII
                     EKDKDHLISIMQKAMKSLDVFLSSYTDDGACNEGPGYWKGASLAYIEIIHKLSELYSI
                     KPDHIINTKIKNMGSYIYKVFIHKDRFLSYADGTGKNPMFDAKLYLAGKMLNDQSMMD
                     MAIDVFRNNRKANIYYDHFIYILSDYIEYIFSYNQLCKESAKPMGTSHYLASSVLTDS
                     HIFTARIKQESHEGFFIGLKGGNNDESHNHNDVGCPYIYLDGEPVVIDPAAGTYTSKT
                     FSNKRYEIWTMQSIWHSLPKINGIMQNAGARYQSESFQWNEDENYAWASLEIAKAYPK
                     ESGIKSYIRKIRLNRKENKAYINDSLILDNPSESIVFHITSFSKPMLVRPGEILLKNE
                     HTSVRLLYNHSLVLFDCTEMDLAYDTKLSNSWGNSIYRADFKVKNVSDKFDLEFCFIQ
                     SYL"
     gene            complement(5821..6129)
                     /locus_tag="PLQ68_05135"
     CDS             complement(5821..6129)
                     /locus_tag="PLQ68_05135"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HXK72066.1"
                     /translation="MIDANLASDLTLHTLASMQNISAGYLSSLFKKEIGCTLTDYVNR
                     KRMKYAAQLLTTTMLQVQTISQYCGIQDLNYFSKIFKRYYGSAPTEYRQVHQVHIVHE
                     "
     gene            6116..7276
                     /locus_tag="PLQ68_05140"
     CDS             6116..7276
                     /locus_tag="PLQ68_05140"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXK72067.1"
                     /translation="MASIIFIGSLGLIIGGALCFIPWRNAPEIAKIIIFICGYVIWDA
                     FYTIANVPYGSLLSLISKEPADRASLSAWRSVGAMAGNILPMVILPIIIYDSNNNIVG
                     ERVFIAALLMGVLGFIAFQFMLRNTEIRVEETVTLNEKRPKFNVFKAIGNFMRNRPAV
                     GATVAAMGMFLGMQGAATAVVVLFQSYFQNVKILGIVQLFAMLPIFLFTPLARKMVVK
                     YGKKELSVIGSLCSVVACIAMIVLPITPDAKGLIIYVLCLFISSLGLGIYSTVSWAMM
                     GDAIDYNEWKNGVREEGVTYSLHSFFRKLAQGIGPAIALVIMVALGYVEANEGSQTFE
                     VALRMRYLVAGLYTFSAILMYVGLGLIYNLDKKKLTKMNEELDSARSKLETE"
     gene            7291..9540
                     /locus_tag="PLQ68_05145"
     CDS             7291..9540
                     /locus_tag="PLQ68_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HXK72068.1"
                     /translation="MRTIYNLNIKWAFSKQVDTVPKELPMNWEWVNLPHTWNATDGQD
                     GGNDYYRGACYYVKQINRTDLPQTDCYYLEIKGANSSADVFLNGKHLAHHDGGYSTWR
                     VDITNDFNDENLLVIAVDNSPNEKVYPQVADFTFYGGLYRDVNLICVNKSHFSLGYYG
                     GPGLKITPEVFDNDAKVQIETWITDYKEGQNICYTIYDKEENKIASTETDKLSAILEI
                     ENVHLWHGRKDPYLYRVEVELLENNEVIDNISSNFGCRTFKIDPDNGFILNGEEYPLR
                     GVSRHQDRLGYGNALLPEHHKEDMELIYELGCTTIRLAHYQHDQYFYDLCDEYGMVVW
                     AEIPYISQHNPLANENTISQMKELITQNYNHPSIVVWGLSNEVSMKGDKDPDLIQNHN
                     ILNDLSHEMDKTRLTTIAAVSPCPIDSPYIKIPDVIAYNHYFGWYGGEASMNGPWFDN
                     FHKKNPSIPIGCSEYGCEALNWHTSQPVQGDYTEEYQAYYHEELIKQLYSRKYIWATH
                     VWNMFDFGADARSEGGENGQNHKGLVTMDRKYKKDSFYAYKAYLSDEPFVHICSKRYV
                     NRVEDVTKITVYSNQPEVELFANGVSIAKKEGKHFFYFEVPNIGKTILTAVAGNLKDE
                     STINKVEEFDESYRMKEKNAILNWFDITMPEGYFSLNDKIGDIMETIRGKLLFMRLIT
                     KMLPKKGKKVAGGFEMNDSMMKMLGGFTVLRLSGMIGMVGVKLTKEDLLSLNAKLNKI
                     KKKQKTKTD"
     gene            9775..10026
                     /locus_tag="PLQ68_05150"
     CDS             9775..10026
                     /locus_tag="PLQ68_05150"
                     /inference="COORDINATES: protein motif:HMM:NF013053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HXK72069.1"
                     /translation="MNKALLIIDMQLMPFLWKNYGGKEIYNEQKLLDNTQKLIEKARI
                     CEVPVYYILFTEQVGSMRAKTNLYGKFIHKLHQKKRTGS"
     gene            10023..10310
                     /locus_tag="PLQ68_05155"
     CDS             10023..10310
                     /locus_tag="PLQ68_05155"
                     /inference="COORDINATES: protein motif:HMM:NF013053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HXK72070.1"
                     /translation="MVKYHADSFFVTDLHALLQKDGITDIIVCGVQTEFCVDTTIRSA
                     FSYGYHSILAMDAHSTFDSELLKAETIISHHNSILTQFTDIITTDDIDFHK"
     gene            complement(10342..11379)
                     /gene="fbaA"
                     /locus_tag="PLQ68_05160"
     CDS             complement(10342..11379)
                     /gene="fbaA"
                     /locus_tag="PLQ68_05160"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020590210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HXK72071.1"
                     /translation="MPIVNYEKYCRMLDYAYANKFAYPAINVASIESANAVLAGFAEA
                     KSDGIIQVSTGGGEHASGQYLKDMALGAVSLADHVHLMAEKYNIYVALHTDHCKPEKI
                     DKFLKPLIEETIKRRARGQMNLFSSHMFDGSDLPLDENIRISKDLLKICAENEIILEL
                     EAGVVGGEEDGVNNSGVSHTKLYTTAEDMLKVYEAMKDIENAKYMFAATFGNVHGVYK
                     PGNVKLKPVILKEGQEALKIKHGEDAKFYLVFHGGSGSGKDEIAETLNYGVIKMNIDT
                     DTQYAFTRTIADHMLKHYDEVLKIDGEVGNKKMYDPRSYLKVAEKNMTKRVIEACNDL
                     RSAGNSLFGKL"
     gene            11606..12880
                     /locus_tag="PLQ68_05165"
     CDS             11606..12880
                     /locus_tag="PLQ68_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006805238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXK72072.1"
                     /translation="MNQKESFNLEFKQETSKTFLKTVSAFANYNDGRILFGIDDDGNM
                     AGMDNAKTESLKIENMINDSISPIPNFDIQVNKNGNKEIVILNVKKGKDTPYYYKGKA
                     YRRSDTSTVEIDRFELRRLAMQGMNIDYENRIASSQLLSFNTLETRLKEELGIKKISL
                     DILKTLNLYSLDGYYNIAAELLADQNSIEFSGVDIVKFGKDTNTFLYRENVNKKSLLI
                     QFYRAIELYEQYYCYEEIEEFNRVKKELIPKEAFREALANAMVHRVWDINSFIQIAMY
                     DDRIEISSPGGLPHGLSEDEYLLENISVLRNPIIASIFNKLHIIEKFGTGITRIKEQY
                     SGCISKPSFSISENRIKIILPVTDKRILQLSDDENSVYALLKNKTLLSRNEIDLKLGF
                     DKSKTIRILNSLITKNIIDKDGKGLAVRYKLK"
     gene            12939..13493
                     /locus_tag="PLQ68_05170"
     CDS             12939..13493
                     /locus_tag="PLQ68_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013281316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXK72073.1"
                     /translation="MQYEITHLPKEKWEGTIVPIGYTTHKYYDVTVNKKDNGFTIDIE
                     KKDFDKTYVFTPEKYNYPDKLYEKHWENACASGVLVNNELIAVIETDQELWSNRLRIT
                     ELWVHEDYQKIGLGHALIEKAKEQAIKEKRRAIILETQSCNVNAVDFYMHEGFSLIGF
                     DSCCYSNNDLDKKEVRLEFGWFNN"
     gene            13592..14362
                     /locus_tag="PLQ68_05175"
     CDS             13592..14362
                     /locus_tag="PLQ68_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015043315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HXK72074.1"
                     /translation="MTYNQIVALWQSYNISSVNDLQKYLDNFRILFAYNSGRIENDEV
                     NYHDTREIFENGKVLNYTGNTRTLFEQQNQKACYDFLIHKVVKKEPLSIELIQEIHRI
                     LTCGTYHEQRFIMNNERPGEFKKHDYITGVYVVGSSCEHVLEDMTNLINEVNSYMGKD
                     ILKAATYLHAKFEYIHPFADGNGRIGRTILNYYLMINNHPPLIVYDEDKYEYYNSLQE
                     YDKTESLTSLFNFFKKETIKTWKKSLSLTESKKIKDKD"
     gene            14447..14896
                     /locus_tag="PLQ68_05180"
     CDS             14447..14896
                     /locus_tag="PLQ68_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017415341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HXK72075.1"
                     /translation="MERSEKVELTVLCMIYNEDTILLQNRIKDDWKGYTLPGGHVEPG
                     ESFVNATIREMKEETGLDIKNPVLCGIKHFPSNDGRYIVLLYKTNKFSGQLSSSSEGE
                     IKWIKRSEIPSLVCVKDFEKLLNVFDNEELSEFQYINRNNLFVAEVL"
     gene            14899..15516
                     /locus_tag="PLQ68_05185"
     CDS             14899..15516
                     /locus_tag="PLQ68_05185"
                     /inference="COORDINATES: protein motif:HMM:NF017959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HXK72076.1"
                     /translation="MNRIKKALTDFKYILYASLLLFIVMLALPFFSAESYSIIKNTTS
                     QLGAQNTVNAWIMNVTFIIVGVSCIAEAWLHLKQFWFQKIVLSIFGLGLIFTGLFSHA
                     PITDTVVFNPIEDQLHSVFASAVGFSFIIYAISSAFIENKKTSRILDLSVGLSTAALS
                     LLMLFYPDFLGIWQRTMFIISFIWLIFMLERIKNLDKNVLHHNQK"
     gene            15643..16764
                     /locus_tag="PLQ68_05190"
     CDS             15643..16764
                     /locus_tag="PLQ68_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014520971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HXK72077.1"
                     /translation="MKISKKDAYTWFKFFASLPEDEQLMADQQEIVYSTFAQIEEVVD
                     KRNAKLMSGIKGLKTLKNRTYYVGNDDRFPNGCKSCLLGTGLSAIRKTNKCNIECKFC
                     YNYGELDDIPPVGEGMWEIGGTKFYEKDIDLLLAIHQKPTGISYVYLEPFMEIEKYYA
                     VIEKFHNAGIHQHMYTNGLLVTEESAKALAKAGLDELRFNLGATNCSDKVIENMKTAS
                     KYINNVGIETPMTPQFYSSFFEKKDKILNSGITFINCAELHLNPNNIENYYGENMYIC
                     RHGYISPIWSRELTLKFMKIADDEKWDLVVHDCSNYTKFTRGLNLTAKEGKWFGANDY
                     ICEFPVIPYEYFLPILKDKSFKFITEENLPKGYAPDEMI"
     gene            16761..17270
                     /locus_tag="PLQ68_05195"
     CDS             16761..17270
                     /locus_tag="PLQ68_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008819382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXK72078.1"
                     /translation="MIKTERLTIKSYDKKDQDDMINILTNDIVKKTFVIPDFKSHQEA
                     VHMFNKLMNYSYSDEHYEKGIYKDGVLIGFVNDVEIDKDTIEIGYVIHPDHHNKGYAT
                     EALTAVIDDLFKKGFRSITAAAFENNIASRRVMEKCGMKLTDKTSIMIHNGILQNCLY
                     YSIYCKSSD"
     gene            17350..17685
                     /locus_tag="PLQ68_05200"
     CDS             17350..17685
                     /locus_tag="PLQ68_05200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72079.1"
                     /translation="MIKWKLNKKTVLYLIIAFLWTWFAWISAYLLSHFNGNTLSTEST
                     VFTLLKDFSVSNQFLPQLLFELAVYGPFIGFLVTGGRKNIINNPNSSKKLWPYNTYFT
                     TICNTVNNP"
     gene            17717..18190
                     /locus_tag="PLQ68_05205"
     CDS             17717..18190
                     /locus_tag="PLQ68_05205"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HXK72080.1"
                     /translation="MPFITLMTMILIYFLSNIITSGTEEFGWRAFLYPEMKKSGMSFW
                     NIAFKGGLIWAVWHYPLLIIMYLPAGIGVLIPSLIGFTASIIAMNYITNFLYEKMNNI
                     WAMVLLHALNNTMSFIVVLLFPKTPFTILSSIMAWVIVWRIEKKYPRILTEDKIV"
     gene            18310..18948
                     /locus_tag="PLQ68_05210"
     CDS             18310..18948
                     /locus_tag="PLQ68_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020816092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HXK72081.1"
                     /translation="MNKITRYLYPLGMIAVILYFSHTILGNLLWKEYNPVTTDISSLT
                     ADGAPNAQLLRVITLFYSIFIILMVVGLVIKAFKSYNRLLKAGFIILLVMQLTSAIGY
                     SLFPLTGDKTDMSFQNMMHIIVTVIVVFSTITFSFLVAFGYIKQEHTKKLGKITLVFA
                     IFVTLFGLFNPISMGMQLNILGLTERLVIYTIQAFMFILSYYYTFIDKEPVT"
     gene            complement(18993..20378)
                     /locus_tag="PLQ68_05215"
     CDS             complement(18993..20378)
                     /locus_tag="PLQ68_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010166779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HXK72082.1"
                     /translation="MKRPNMLFIMSDDHAAHAISAYKSRINKTPNIDRIANEGMIFDN
                     CFCTNSICAPSRATIISGTHTHINGVTKLNIPFDGRQPTFPKILSEGGYTTAMIGKWH
                     LGHGGNSDPTGFDYWNVLPGQGEYFDPVMIEMGEKKKLSGYVTDIITDLSINWLENRD
                     KTKPFMMMCHHKAPHRPWIPDKAHKDMYENEDIPEPETFNDNYENRSSAAKEAWMRVD
                     KNLNFYDVKQQEPTGLSDAQLKSWKYQRYIKDYLKCIASIDDNVGRILDYLEDNNLYE
                     DTIVVYTSDQGFFLGDHGWFDKRLMYEESLRMPFVMRYPRMIKPGSRSDEIVLNLDFA
                     QTFIDFAQLDADPVMQGRSFKKIAAGENVDGWRKSMYYRYYDFPSMHNVYPHYGIRTK
                     KHKLICYYPAGESSEEGMKPLRDIEWELFDLEKDPQEMHSVYFDEHYKDVVDKLKKEL
                     AAIKYEAKDFD"
     gene            20576..22999
                     /locus_tag="PLQ68_05220"
     CDS             20576..22999
                     /locus_tag="PLQ68_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HXK72083.1"
                     /translation="MQDFEKLGVFYLGKKYDIDNNKLKEDYVLLKSKDLTTHAVIIGM
                     TGSGKTGLGIGIIEEAMMDNIPVIAIDPKGDLTNLALTFPLLRGEDFLPWINLSEASN
                     KGMAPEEYAKAQADSWKKGLAEWNQDGLRIQGLKNESDVKIYTPGGSSGIGVSVLKSF
                     NAPSKEVLADKDTYTDRISATTTSLLSLLGINADSFTSREFILVSKILDNSWTEGKSL
                     SLPQLIAEIQNPPFDKIGVMDINSIYPLKDRFTLAMLLNNLIAHPGFEAWLEGEPLNI
                     DKFLYGNTGKPRVSVFSISHLSDSERMFFVTMLLNEVLSWARSQPGTGSLRAMIYMDE
                     LFGYLPPVSNPPSKAPLLTLLKQARAYGLGLVLSTQNPVDLDYKALSNAGTWFIGRLQ
                     TERDKQRVIEGLEGAAAGGSFDKNRTEQILAGIGKRVFYLHSVHEDEPAIFNTRWVMS
                     YLAGPLTKNQISLLPSSNITSDIPITTDQFKQQEQLNTQIDETVSTSVVLSPSIKQVY
                     LPEKIRSRGKLVYKPAVIGVADVLYNNEKHMITATKNYTILTQFNDSPVPLDWNQSQI
                     IDMDINDIETTAIEGALFANYPSSAANAKNYDTWKKLLSQHIRTNLSLKLYSSPKLKM
                     VSEYGEEERDFLIRVQHSAHEKRDLEIESMKKKYSSKIQTLENALIRARQAVEKQNAM
                     ASQKKMDAMVSAGSAILGALFGRKTISSTSVSKIGTAVSRTSKAMKSGQNIDQAEERL
                     QALEVQYENIQIELEQKLKDVSSEYDLIKNHIETINIRATSTNITIHLLGLAWVPYEK
                     GDIKDTILI"
     gene            23179..23784
                     /locus_tag="PLQ68_05225"
     CDS             23179..23784
                     /locus_tag="PLQ68_05225"
                     /inference="COORDINATES: protein motif:HMM:NF014068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HXK72084.1"
                     /translation="MGKKVVLLVLDLFADWEGAYISTMLQDNNISIKSQVMWASIDKK
                     TKKSMGYMTMIPDITLEEIPDDIDALILIGGKSYRSNDADKLTFYIDKFIKAGKVVGF
                     ICDATYYAAKQGFLNNVKHTGNGLHEMIKLREYTNSDNYINEDAVSDNNIITANGTSP
                     VVFASLILKALNFLPEKEIEFMTDMHLIGYMNALIKHGYIN"
     gene            23905..24498
                     /locus_tag="PLQ68_05230"
     CDS             23905..24498
                     /locus_tag="PLQ68_05230"
                     /inference="COORDINATES: protein motif:HMM:NF022636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HXK72085.1"
                     /translation="MKVPNLVKYSKLSKSTYGIINVLFWILFALTILVILLTIATRVF
                     PNIINLSDSDGSFRISPDNIIQFEISTGAISNTRIVEIYSLMFAGSALLLILILLVLS
                     QLKIILKNVVNKNPFAEKNAKSIKNIGYVIMASSLVMPVTKAFLINKIIHAFNLSNVN
                     VVYTVNIEVLIIGILLLLVSAIFNYGAYLQQEYDTTL"
     gene            24514..24747
                     /locus_tag="PLQ68_05235"
     CDS             24514..24747
                     /locus_tag="PLQ68_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009595835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HXK72086.1"
                     /translation="MAIIIRLDRVLADRKMQLNELADSVGISIVNLSNLKTGKVKAIR
                     FSTLNEICKILKCTPGDILEYEYTENESSPEED"
     gene            complement(24779..>25010)
                     /locus_tag="PLQ68_05240"
     CDS             complement(24779..>25010)
                     /locus_tag="PLQ68_05240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72087.1"
                     /translation="RRAIMDLKKKIDEIVKKIKADDEFGKKFKDDPVKAVESVLGVDL
                     PDDQVNKLIDGVKAKLNLDKADGLFGKIKKLF"
BASE COUNT         8846 a         3932 c         4388 g         7744 t
ORIGIN      
        1 atccttatgg acaagaataa gtacgaaaag gaatacatag ttacagttga taaggaaata
       61 acttctgcat ttatagaagg tatgtcaaaa ggagtaagaa tatttaatcc tgtaaaaaat
      121 aactatacac gtacaaaccg atgtaaaata ttcaaaattg attcccgaac tttcaaaata
      181 gtacttacgc agggacttaa tcggcagata agaagaatgt gctcgcattt tggttatgaa
      241 gtaattaagc taaaacgtat cagaattatg gatatcagcc tgaaaaacct tccagttggc
      301 aagtggagaa acttaacaaa tgaagagatg aaatctttat tggctgcaaa aaaagcagat
      361 aaaaaaaatg ataaataaat ctgtaaacag gagcagctgg aatttttatc cacaataaac
      421 tattgaaaac atttaaaaaa gtatatatag aaattaccaa catatgcaat atgaaatgcg
      481 atttctgtcc aaaaacaaac agacctctag aatttatgaa cactgaaaca ttcagtaaaa
      541 tcttatatca gataaaacct cacaccaact atatttactt acatgttaag ggagaacctc
      601 tgctccaccc tgaattggat aaaattctgg atataagtta cgaaaaggga tttcatgtga
      661 atataaccac aaatggaaca ttaataagtt cagtaaaaga tatgcttatg actaaaccag
      721 ctgttagaca aataaatttt tcactacaca gcattgatgg aaataagatg tttaaaaatg
      781 aactggcata catagaaaac atactttctt ttacaaaaga atcaattgaa aaaacagaaa
      841 tgattgtttc ctttcgcttg tggaatttaa atgaagataa tgcaaccacc ttaaaagtca
      901 gaaaaaacaa caatatttta gaagctatcg aaaccttctt taaattacaa tacagcatcg
      961 aagaaactaa aggtataaaa agtggcataa aaattgctga aagggtatat attaatcaag
     1021 cacagcagtt caaatggcct gacataaatg aaaaagaaat aaatgaatgc ggtttctgtt
     1081 acggtcttcg taatcaggct gcaattcttg tcgatggtac agttgtacca tgctgtctgg
     1141 ataatgatgg agtgatcaat ttaggaaaca ttcacaataa gagtttttca gaaataattg
     1201 aaagcgaaag agcaaacaac atagttaatg gttttacaaa aagacatgta gttgaagaac
     1261 tctgtagaaa atgcggatat atgcgtcgtt tttaaaacat taattcaaac acaatattca
     1321 cttgttgcaa ttgcaacata catgatttac ccttcctgtt atacttcatt taagctaata
     1381 ctgatgaaaa actataataa ctaggatggt ataaaaaatg gcaaaagaaa aaatattcaa
     1441 caaagaagaa caacagatta tcaaaacact cagacaattc gtacctgttg ttgacagagt
     1501 tcatggcagt acgcatcctg aatttcatga agtaaaaaga ctttttgagg ctatagatga
     1561 aaaagtgaaa agtaatgctt cagaaaaata tcaacttact gaggaatttg caaagctgtc
     1621 tgaaatcaca agtaattata ctgttccgaa tgatgtatgc gagagttatg aagcagtata
     1681 tgtaatgctt gctgaactta atgaagcata taatgagata aataaataat aaaattagat
     1741 aacacgattg gagaagcaag gtgctaagat tcatattaag taaaaagaac catataacac
     1801 tgataagcgg aatactgatt gcatttgctt actttagtga gcttgtgcta aagaatgaaa
     1861 ctctttttag atggcctatg ataatagcat cggttattgg agttatacca atcgcactnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngg atacattaat gaggctagta
     2041 taacaggaga atcttttcca gttagcaaag caaaaggttc tttagtttat gcaggaacta
     2101 tccttgaaaa cggcacctta cagataactg ctgaccgagt aggagaagac actacttttg
     2161 gtaagattat tgaattagtt gaagaggctc aggattccaa gtctgaagct gagcggttta
     2221 ttgataagtt ttcaaagtac tacactcctg cagttctggt tttagcttta ttcgtctgga
     2281 ttatatctaa aaaccttgaa cttgcaataa caatacttgt attaggatgt ccaggagcac
     2341 tggttatagg tgtaccggtt tcaaatgttg caggtatagg aaatggagca cgacatgggg
     2401 tattactaaa aggcagcgaa gttgttcagg acttcagcag aactaataca atagtttttg
     2461 acaaaacagg tactctcaca ataggcagac ctaaagtagt tgatattaaa acatacacaa
     2521 aagaaataga aactgcatta agttatctag taagcgtgga aaacgaatcg gatcatctgt
     2581 tagctaaagc agtcatagaa tatgcaaata acccaaaata ctatcctgta gaaaatacag
     2641 atgtcataaa aggaaaagga attgtagctg atgtagatgg tcatagagtt gctgtaggta
     2701 atatggcttt gatggaacat gaaaaagtta atataagtaa agaaatgtac gaagatatat
     2761 ccagatttga acaaaatgga gactcacttg tactaacttc agttgatggt gaattaaata
     2821 tattaatggg tatacgcgat cagctcaggc ctgatgtaaa gaaccatctt caaaaactta
     2881 aagcattgag agtaaaaaac cttattgtcc tatcaggaga taatcaatct acagttgaca
     2941 aagtagcaga ggagctcggc ttgacggaag cttatgggaa catgcttccg caggataagt
     3001 cgcagtttat taggaatcta caggaaaatg gtcacaaagt ggcatttgta ggtgatggcg
     3061 taaatgacag tccttcgtta gcattagcag atataggaat tgcaatgggt ggaggtacag
     3121 atgtagcgat tgaaacttct gatgtagtac ttatgaattc agatttttcc cgcctgccac
     3181 atgccttagg tttagcaaaa gcgactgcca gaaacatgat acagaacatt gtaattgcag
     3241 taggagtagt actatttctt cttgctaatg ttttcttcag tgagtggatg aacatgtcaa
     3301 taggaatgct ggtacatgaa gcaagcatct ttgttgttat acttaatggc atgagactgc
     3361 ttggttacaa atcaagataa aataaaatta atgaaaggaa gatataatgc taatgcatta
     3421 tactggttaa aaaaatgaaa aaagcaacaa tacagttaga gacattaacc tgtccgtcat
     3481 gtgcacttaa gatagaaaaa gctgtaaaat cactaaatgg agtagataaa gatagtgtta
     3541 acgtagcatt catgtcaagc aaagtaaagc ttgagtttga tgatgataag ttaaaagttg
     3601 atgaaattga aaaagccatt acatctttag gctatgaagt aaagaaaaca caagtaaaag
     3661 cctagttgga tatagctatt aaaattttag aaagagagta atactctctt ttctattttc
     3721 ttttttttta tttttttctt ttattttttc tctttccttt tttctatttt cttttttctc
     3781 ttttctttta tatttcatta acatattaaa tgtttatgat agatacttgt tgaaaatata
     3841 aatggtttac ctttttacct caattatgta aaataaaatt aaggaaggta ttaatgttat
     3901 cagaactgct taataagaat ataattcaaa agactgaacc tggcatgttt tcactgttcc
     3961 ctgtttacga ggacagaaac gcctgggaat caatagatca caaattgaaa actcatataa
     4021 taaatgaagc agaaggttta atcagacata cttggcctcg tatttgtgat gaagattacg
     4081 aagattatca taaaacagga agcagaaaaa agtacgatga gaaatttaag aatttatatg
     4141 attccttaac aaaaattgta ttagctgaat gtattgaggg aaagggccgt ttcattagag
     4201 atattgcagc aggaataact acccgtttaa atgaaactgc ctggattaat cctgctcact
     4261 taaaacatta tggagaggat ataataccag ttcctgatgg gaaatatctt gatttaagat
     4321 gctgcagtac agcaaaacag cttgctgtaa tatattatct actaggtaaa aaacttgctg
     4381 aatatgaacc tcttcttcta gacagaatgg aaaaggaaat aaaaataagg gtactggaca
     4441 gatatattga tacagatcag tggtggacca acttatctga aggtttcgtt ataaacaact
     4501 ggaacacgca ctgcaataaa tgtctaatca ttacagcgat gataattgaa aaagacaaag
     4561 accatcttat aagcataatg caaaaagcaa tgaagagcct tgatgtgttt ctttcttcat
     4621 atactgatga cggagcatgt aacgagggcc ctggttactg gaagggagct tcattagctt
     4681 acattgaaat aatacacaag ttatcagaac tttattcaat taaaccagat catattatta
     4741 atactaaaat taaaaacatg ggaagctata tatataaagt cttcatacac aaagacagat
     4801 ttttaagtta cgctgatggt acaggaaaaa atcctatgtt tgatgcgaaa ctgtacttag
     4861 ccggtaaaat gctaaatgat cagtctatga tggatatggc aattgatgta tttagaaaca
     4921 atagaaaagc aaatatatat tatgatcatt tcatatatat cctgtctgat tatatagaat
     4981 acattttttc atataaccag ctttgcaaag aatccgctaa accaatggga acgtcacact
     5041 accttgcatc ttctgttctt actgattcgc atatatttac agcccgaata aaacaagaaa
     5101 gccatgaagg gtttttcata ggactaaagg gcggtaataa tgatgaatct cataatcaca
     5161 atgatgttgg atgcccatat atatatcttg atggagaacc tgtagtcatc gaccctgctg
     5221 cagggacata tacttcaaaa acatttagta ataaaagata tgaaatatgg accatgcagt
     5281 ctatatggca tagtttacct aagataaacg gaattatgca aaatgctgga gcgaggtatc
     5341 agtcagagtc ctttcaatgg aatgaggacg aaaactatgc atgggcatct ttagaaatag
     5401 caaaagctta tccaaaagaa tccggtataa aatcttatat aaggaaaata agacttaaca
     5461 gaaaagaaaa taaggcatat ataaatgaca gccttatact tgataatcca agcgaaagca
     5521 ttgtttttca tattacttcg ttttcaaaac ctatgcttgt cagacctggc gaaatacttt
     5581 taaaaaatga gcatacaagt gtaagattgt tatataacca ttcgcttgta ttgtttgatt
     5641 gtactgaaat ggatttagct tatgatacta aactttcaaa ttcatgggga aatagcatat
     5701 acagagcaga tttcaaggtt aagaatgtat cagacaagtt tgatcttgag ttctgcttta
     5761 ttcagagtta tttataaata caaggttatt atcatatata aatccagcct taatattaca
     5821 ttactcatgc actatgtgaa cttgatgaac ctggcgatat tcagtcggag cagacccata
     5881 atatcgtttg aagatttttg aaaaataatt aagatcctgt ataccgcagt actgagagat
     5941 ggtttgaacc tgaagcatag tagtggtaag aagctgagcc gcatacttca tacgctttct
     6001 atttacatag tcagtcagag tacaaccgat ttctttttta aataatgaag acaaatatcc
     6061 tgctgaaata ttctgcatag aagctaatgt atgtaaagta aggtctgaag caagattggc
     6121 atctatcatt tttataggtt ctttaggact tatcatcggc ggagctttat gctttatccc
     6181 ttggagaaat gcaccagaaa tagcaaaaat tataatattc atatgcggtt atgttatttg
     6241 ggatgccttt tatacgatag cgaatgtacc ttatgggtct cttctatcgc ttatctcaaa
     6301 agaacctgcc gacagggctt ccttgtcagc ttggcgttca gtaggtgcaa tggctggtaa
     6361 tatactgcca atggttatat tgccaataat tatatatgat tcaaataaca acattgttgg
     6421 tgagcgtgtc tttatagctg ctctgcttat gggagtgtta ggttttattg catttcaatt
     6481 tatgctacga aatacagaaa tacgtgttga ggaaacagta acactaaatg agaaacgtcc
     6541 taaattcaat gttttcaaag caattggtaa ctttatgcgt aatcgtccag ctgtcggtgc
     6601 gactgtagca gcaatgggca tgtttcttgg aatgcaaggt gctgcaactg ctgttgtcgt
     6661 acttttccag tcatattttc agaatgttaa aattttggga atagtccagc tttttgcaat
     6721 gcttcctatt tttcttttca cacctttagc acgtaagatg gttgttaagt atggcaaaaa
     6781 ggaactgtca gttataggtt ctctttgtag tgttgtagct tgtattgcaa tgatagtact
     6841 tccaataact cctgatgcaa aaggccttat aatctatgtc ttgtgcttat ttataagcag
     6901 tttgggttta ggaatatatt caactgtctc atgggcaatg atgggtgatg ctattgatta
     6961 caacgaatgg aagaatggag tcagagaaga aggagttact tattctttgc attcattttt
     7021 tagaaaactt gcacaaggta ttggaccagc aatagctctt gtaataatgg ttgccttagg
     7081 ttacgtggaa gctaatgaag gcagtcagac atttgaagtc gctttaagaa tgcgttatct
     7141 tgtagctggc ttatacactt tcagtgctat tttgatgtat gtgggtctgg gactcattta
     7201 caatctcgat aaaaagaaat tgacaaaaat gaacgaagaa ttagattccg ctcgctctaa
     7261 gttggaaaca gaataaaggg aggtcttttc ttgagaacaa tatataattt aaatataaaa
     7321 tgggcttttt cgaaacaggt tgatacagtt ccaaaagaac tgccaatgaa ttgggaatgg
     7381 gtcaatctac cacacacctg gaacgcgaca gacggacagg atggaggcaa tgactactac
     7441 cgtggtgctt gctattatgt aaaacaaatt aatagaacag atttacctca aacagattgc
     7501 tactatctag aaataaaagg agcaaattcc tctgctgatg tttttcttaa tggaaaacat
     7561 ttagctcacc atgacggagg ttactcaaca tggagagtgg atattaccaa tgacttcaac
     7621 gatgaaaatc tgcttgttat tgctgttgat aactcaccta atgaaaaagt ttatccgcaa
     7681 gtcgctgatt tcacattcta tggaggtctg tatcgtgatg tgaacttaat ctgtgtaaac
     7741 aaatctcatt tctctttggg ctattatgga ggtccagggt taaaaataac tcctgaagta
     7801 tttgacaatg atgcaaaagt acagatcgaa acatggataa ccgactataa agaagggcag
     7861 aatatttgtt atacaattta tgataaagaa gagaataaga tagctagcac agaaactgac
     7921 aaattgtctg caatactgga aatagaaaat gttcatctct ggcatggccg taaagatcct
     7981 tatctgtaca gagttgaagt agagcttttg gagaataacg aagtaattga caatatatct
     8041 tcaaattttg gatgcaggac ctttaagatt gatccggata acggttttat cctcaatgga
     8101 gaagagtatc ctttaagagg agtatcacgt catcaggacc gtttaggtta tggaaatgct
     8161 cttttacctg aacatcataa agaggatatg gaacttattt atgagttagg ctgtacgaca
     8221 atacgtcttg cccattatca gcatgaccag tacttttatg atttgtgtga tgaatatgga
     8281 atggtagtat gggctgaaat accatacatc tctcaacaca accctttagc aaatgaaaac
     8341 acgataagcc agatgaagga gcttattaca caaaattaca atcatccttc aatagtcgta
     8401 tggggtcttt caaatgaagt gagcatgaaa ggagataaag atcctgacct tatacaaaat
     8461 cataacatac ttaacgatct ttctcatgaa atggataaaa ctcgtctcac tactattgct
     8521 gctgtctccc cttgtccaat agacagcccc tacataaaga tacctgatgt tatagcatat
     8581 aatcactatt tcggctggta tggcggagaa gcttcaatga acggtccatg gtttgacaat
     8641 ttccataaaa aaaatcccag tatacccata ggctgctctg aatacggatg cgaggcattg
     8701 aactggcata cctctcaacc tgtacaagga gattatacag aagaatacca ggcatattat
     8761 catgaggaac tcataaagca gttatattca cgtaaatata tctgggcgac tcatgtctgg
     8821 aacatgtttg actttggtgc agatgcaaga agtgaaggcg gcgaaaacgg gcagaatcac
     8881 aaagggcttg ttacaatgga ccgcaagtac aagaaagact ccttttatgc atacaaagca
     8941 tatttatctg acgaaccatt tgtccacata tgctcaaaac gctatgttaa ccgagttgag
     9001 gatgtaacta aaattactgt ttattccaat cagcctgaag ttgaactatt cgcaaatggt
     9061 gtatcaatag ccaaaaaaga aggtaaacat ttcttctatt tcgaagtacc aaatataggt
     9121 aaaactatat taactgctgt agcaggaaat cttaaggatg agagcacaat taacaaagtt
     9181 gaagaatttg atgaatcata ccgtatgaaa gaaaagaacg caatacttaa ctggtttgac
     9241 attactatgc ctgaggggta tttctctctt aatgataaaa tcggagacat tatggaaacc
     9301 atcagaggca aacttctttt catgagatta attacaaaaa tgcttccaaa gaaaggcaaa
     9361 aaagtagcag gcggttttga aatgaatgat tccatgatga aaatgcttgg aggattcaca
     9421 gtactgcggt taagcggaat gatagggatg gtgggagtaa aattaacaaa agaagactta
     9481 ctcagcctaa acgcaaaact caacaaaata aagaaaaagc aaaaaactaa gaccgactga
     9541 ttattatcaa ctgataacaa ccagaaatat tcataataat tattcattgc ttaacagaca
     9601 ttgccaagta gacaatgtct gttagacaat gtctgttgaa caataaattt tagtatcaac
     9661 ctcacagttg cttttacctt acatcatttt gctataaaca gatagataac taatgtagaa
     9721 ttatgataga atatctaccc agaaagacat tttgattatt ttcaggagaa tgaattgaac
     9781 aaagcattat tgataataga catgcagtta atgccatttc tatggaaaaa ttatggcgga
     9841 aaagaaattt acaacgaaca gaaactactg gataatacac agaaactaat agaaaaagcc
     9901 cgaatatgtg aagttccggt ttactatatt ctttttactg aacaagttgg ttcgatgaga
     9961 gcaaaaacca acctttatgg caaattcatc cacaaattgc accaaaagaa aaggacagga
    10021 tcatagtaaa gtatcatgca gattcattct ttgtaactga tcttcatgct ttactacaaa
    10081 aagacggaat aacagatatc attgtctgtg gagtgcaaac ggaattctgt gttgatacta
    10141 ccataagaag tgctttttca tatggatacc attcaatatt agccatggat gctcatagca
    10201 ctttcgattc tgaattacta aaagcagaaa caataatctc tcatcacaac tctatactga
    10261 cgcagtttac tgatataata actacagatg atatagactt tcataagtaa tcaagagaca
    10321 aataagctta ctgtacacaa tttataactt tccaaagagg ctattgcctg ctgaacgaag
    10381 gtcattacaa gcttctataa ccctttttgt catatttttt tcagcaacct taagatagct
    10441 tcttggatca tacattttct tgttacctac ttctccatca attttcaata cctcatcata
    10501 atgttttaac atatgatctg ctattgttct tgtaaatgca tattgtgtat cagtatctat
    10561 attcattttg ataacgccat aattaagagt ctctgcaatt tcgtcttttc cagaacccga
    10621 acctccatgg aacacgagat aaaattttgc atcttcacca tgttttatct taagagcttc
    10681 ctgcccttct tttaagataa caggttttaa cttcacgttt cctggcttgt acactccatg
    10741 tacatttccg aatgttgctg caaacatata cttggcattc tctatatctt tcatagcttc
    10801 ataaaccttc aacatatctt cagctgttgt atacagtttt gtatgagaaa caccagaatt
    10861 attgacacca tcctcttctc ctccaacaac accagcttct aattcaagta ttatctcatt
    10921 ttcagcacat attttcagca agtcttttga tattctaata ttctcatcta aaggcaaatc
    10981 agatccgtca aacatatggc ttgagaataa attcatctga cctctcgctc ttcttttaat
    11041 agtctcttca ataagaggtt taaggaattt atcaattttc tcaggtttgc agtggtcagt
    11101 atgtaaagct acatatatat tgtacttttc tgccatcagg tgaacatgat ctgctaacga
    11161 aacagcacct aatgccatat ctttaagata ctgtccggat gcatgttctc ctccaccagt
    11221 agacacttgt ataattccat cagattttgc ttcagcaaat cccgctaaaa cagcatttgc
    11281 tgactctatt gatgctacat taattgcagg ataagcaaac ttattcgcat atgcataatc
    11341 tagcattcta caatattttt cataattaac aattggcatc aactatctcc tctttatcgt
    11401 ttttttcatt atatgatgta taaccagatt acatgcataa tactagttca aaatgtatac
    11461 atgaatacac atcgttgcaa atcaaaatgc aacgatagcg attttatcgt tgcattttgc
    11521 aacgataaat gcaacgatac ccttttcgtt gcatattttc aacaactgtg ataatatcaa
    11581 agtataaaat acaggagaaa caataatgaa tcaaaaagaa agctttaatc ttgaattcaa
    11641 acaggaaaca tcaaaaacct ttctaaagac ggtaagcgca tttgctaact ataatgacgg
    11701 aagaatatta tttggcatag atgatgatgg aaatatggca ggtatggata atgcaaaaac
    11761 agagtcgtta aaaattgaaa atatgataaa tgattctata agtccgattc ctaatttcga
    11821 tattcaagtc aataaaaatg gaaataaaga aattgttata ctaaatgtta aaaaaggaaa
    11881 agatactcca tattattata aaggaaaagc atatcgcaga tctgacacat caactgtaga
    11941 aattgacaga tttgaactca gaagactggc aatgcaggga atgaatattg attatgagaa
    12001 cagaatagct tcctctcagt tattaagctt taataccctg gagacaagat tgaaagaaga
    12061 attgggtata aaaaaaatca gtcttgatat attgaaaaca ttaaatttat atagtcttga
    12121 tggatattac aatattgcag ctgaactttt agctgaccaa aatagcattg agttttccgg
    12181 agtggatata gttaaatttg gaaaagacac aaatacattt ctatacaggg aaaatgtaaa
    12241 taagaagtct ctattgattc aattttacag agcaattgaa ctttatgaac aatattattg
    12301 ttatgaagaa atagaggaat ttaacagagt taaaaaagag cttataccca aagaggcctt
    12361 cagagaagct ttagcaaatg caatggtaca tagagtatgg gatattaatt catttattca
    12421 gattgctatg tatgacgata ggattgaaat aagctcaccg ggaggtttac ctcacggctt
    12481 atctgaagat gagtatttat tagaaaatat ttcagtttta agaaatccaa taattgcttc
    12541 catatttaat aagcttcaca ttatcgagaa atttggtact ggcataacaa ggattaagga
    12601 gcaatattcc ggttgcatat caaaacccag ttttagtata agtgaaaata ggataaaaat
    12661 catattacca gtaacagata aaagaatatt acaattgtct gatgacgaaa attcggtata
    12721 tgctctattg aaaaacaaaa ctttgttatc tagaaatgaa atcgatttaa aactcggatt
    12781 tgataaatcg aaaactatca ggatattaaa tagtcttatt actaaaaaca ttatcgacaa
    12841 agatggaaaa ggactagcgg ttaggtacaa actgaaataa taccaatatc tgataatgct
    12901 taaatcaagt ttaatatcat taaattaaag aggtaaccat gcaatacgaa ataacacatc
    12961 tgccaaaaga aaaatgggaa ggtactattg taccgatagg ttatacgaca cataagtatt
    13021 atgatgtcac tgtaaacaag aaagataatg gctttacaat tgatatagag aagaaagatt
    13081 ttgataaaac atatgttttc actcctgaaa aatacaatta ccctgataag ctttatgaaa
    13141 aacactggga aaacgcatgc gcatcaggag tattggttaa taatgaatta attgcagtta
    13201 ttgagactga tcaggaacta tggtccaaca ggctcaggat tacagagtta tgggtacatg
    13261 aggattatca gaagataggt ttagggcatg ctcttattga aaaagcaaaa gaacaagcaa
    13321 taaaagaaaa aagaagagca atcattttag aaacacagtc atgtaatgtt aatgctgttg
    13381 atttctatat gcatgaagga tttagcttaa tcggttttga ttcatgctgt tacagtaata
    13441 atgacctaga caaaaaagaa gtacgacttg agttcggatg gtttaataat tgaatactct
    13501 cttcacttgc ttttatttac ctatataatt ctaaggtaat taattaaata tgatataatt
    13561 acttaagata agcacaaagg agatattcta aatgacatac aatcaaattg ttgctttatg
    13621 gcagtcatat aatatttcat cagtaaatga tttgcagaaa taccttgata attttcgtat
    13681 attattcgca tataactcag gcagaattga aaatgatgag gtaaattatc atgatactag
    13741 ggaaatattt gagaatggta aagtattaaa ttatacagga aatacaagaa ccttatttga
    13801 acagcaaaat caaaaagcat gttacgattt tctaatacat aaagttgtta agaaagaacc
    13861 gctcagtatt gaattaatac aagaaattca caggatttta acctgtggaa catatcatga
    13921 acaaagattt attatgaata atgaaagacc tggagaattc aaaaaacatg actatattac
    13981 cggagtatat gtggtaggtt cttcttgtga gcatgtgtta gaagatatga ctaatcttat
    14041 taacgaagtt aactcatata tgggaaaaga tattttaaag gcagctacct atcttcatgc
    14101 aaaatttgaa tatattcatc cttttgcaga tggaaatgga cgcataggaa gaactattct
    14161 taattattat ctaatgataa ataatcatcc tcctcttata gtttatgacg aagacaagta
    14221 tgagtactat aattctttac aggaatatga taaaactgaa tctctaacca gtctatttaa
    14281 ctttttcaaa aaagaaacca tcaaaacatg gaagaaaagt ttatctctta cagagagtaa
    14341 gaaaatcaaa gataaagatt agaatggaat atatgacata atgaataatc aattggctat
    14401 gattaattta ttatcggtta taaattaaat tcaatggagg aacagtttgg aaagatcaga
    14461 aaaagtagaa ctaacagtat tatgcatgat atacaatgaa gacacaatac ttcttcaaaa
    14521 tcgaattaaa gatgattgga aaggttatac tcttccaggc ggacatgttg agcctggtga
    14581 gtcatttgta aatgcaacaa tccgtgagat gaaagaagaa acaggacttg atataaaaaa
    14641 tcctgttctt tgcggaatca agcacttccc ttctaatgat ggcagatata ttgtacttct
    14701 atataaaact aataaattta gcggtcaatt aagcagttca agtgaaggtg aaataaaatg
    14761 gattaaaaga tctgaaattc cttcacttgt atgcgttaag gactttgaaa agcttctaaa
    14821 tgtttttgat aatgaagaac tttcagagtt tcagtatatt aatagaaata acctctttgt
    14881 tgctgaggta ttataaaaat gaatagaatt aaaaaagcat tgactgattt caaatatata
    14941 ctctatgctt cattactgct ttttatcgta atgctcgcac ttcccttctt cagcgctgaa
    15001 tcatattcga ttataaaaaa cacgacaagc cagttaggag cacagaatac tgttaatgca
    15061 tggattatga atgtaacatt tataattgtc ggagtatcat gcatagctga agcatggcta
    15121 catctgaaac aattctggtt tcagaaaatt gtattgagta tttttggcct tggccttata
    15181 ttcactggat tattcagtca tgcaccaata acagatactg tcgtcttcaa tccaattgaa
    15241 gaccagttac attctgtttt tgcttcagca gtaggatttt cattcataat atatgctatt
    15301 tcatcagctt ttattgaaaa caagaaaaca agcagaattc tagatttatc tgtaggatta
    15361 tcaacagcag ccctttcctt attaatgctt ttctaccccg atttcttggg aatttggcaa
    15421 agaacaatgt ttatcatttc tttcatatgg cttattttca tgcttgagag aataaaaaat
    15481 ctggataaaa atgttcttca tcataatcag aaatgagtaa ttatttttat tattcatatg
    15541 attaaatgca aagtgttata taatatattc tagtccattt aaatcaagtg aaatttatat
    15601 aggatgaaaa taagttagaa agcgatgtgc tcaggagaag aaatgaaaat atcaaaaaaa
    15661 gacgcttaca catggttcaa gttctttgct tctttaccag aagatgaaca gctcatggca
    15721 gaccagcaag aaatagtata ttcgacattc gcgcagattg aggaagttgt cgataagcgg
    15781 aatgcgaaac ttatgtctgg tataaaaggc cttaagactc taaagaatag aacatactat
    15841 gtaggtaatg atgacagatt tcccaatggt tgcaagtcat gtcttttagg gacaggttta
    15901 agcgctataa gaaaaaccaa taaatgcaat atagagtgca aattctgcta taactatggt
    15961 gaacttgatg atatccctcc tgtaggcgaa ggaatgtggg aaataggagg aacaaagttt
    16021 tatgaaaagg atattgatct tcttcttgcc atacaccaaa aaccaacagg catatcatat
    16081 gtctatcttg aaccctttat ggaaattgag aaatattatg cagttataga gaaatttcat
    16141 aatgctggaa tacatcagca tatgtacaca aatgggctat tagttactga agaatcagca
    16201 aaagcattgg ctaaagctgg tcttgatgag ttaaggttta atcttggtgc taccaattgt
    16261 tctgacaaag taattgaaaa tatgaaaact gccagcaaat atatcaacaa tgtcggaatt
    16321 gagaccccaa tgacacctca attctatagt tcattttttg aaaagaaaga taaaatactt
    16381 aattcaggaa taacttttat aaactgtgct gagttgcatc taaatcccaa taacatagaa
    16441 aattactatg gagaaaatat gtatatttgc cgtcatggat atatatcacc tatatggagc
    16501 cgtgagctga cacttaaatt catgaaaatt gcagatgatg agaaatggga tttagttgtg
    16561 catgactgct ctaattacac taagttcaca cgaggcctta atttaaccgc aaaagaagga
    16621 aaatggtttg gagcaaatga ttacatatgc gaattcccag taatccccta cgagtatttt
    16681 cttcctatac ttaaggataa gagtttcaaa ttcataactg aagaaaatct tccaaaaggt
    16741 tatgcacctg atgaaatgat atgataaaga ctgaaagatt aactattaaa tcatatgaca
    16801 aaaaagatca ggatgatatg ataaacatcc ttacaaatga tatagttaag aaaacatttg
    16861 taattcctga ttttaaatct catcaagaag ctgtacatat gtttaataag cttatgaact
    16921 actcatattc tgatgagcat tacgaaaaag gtatatataa agatggcgtt cttattggtt
    16981 ttgttaatga tgtagaaatt gataaagaca ctattgagat aggatatgtt attcatcctg
    17041 accatcacaa caaaggatat gctactgaag cgttaacagc agtaattgat gatttattta
    17101 aaaagggatt cagaagcatt actgcagcag catttgaaaa caatatagct agcagacggg
    17161 taatggagaa atgtggaatg aagctgacag acaagaccag catcatgatt cacaatggca
    17221 tattacagaa ttgtttatat tactccattt attgcaagag cagcgattga atcttaactg
    17281 gaaatactaa tttgttttta ttttaattaa tattataata aaagtaaata atcaattgcg
    17341 ggggcattta tgataaagtg gaaattaaac aagaaaacag ttttgtattt gataatcgct
    17401 tttttatgga cttggtttgc atggatttca gcatatcttt taagccattt taatggaaac
    17461 acattatcaa ctgaaagtac agtttttacg ctattaaaag atttttcagt aagcaatcag
    17521 tttctaccgc agcttttgtt tgaattagct gtttatggtc cttttattgg atttttagta
    17581 acaggcggca gaaaaaatat tattaataac cccaattcgt caaaaaaatt atggccttat
    17641 aatacttatt tcactactat ctgtaatacc gtcaataatc cttagtcttg caacctcatt
    17701 ttacgatgta aaagggttgc catttattac tctgatgact atgatattaa tctacttttt
    17761 atcaaacatc ataaccagtg gcaccgagga gttcggatgg agagcctttc tatatcctga
    17821 aatgaaaaag agcggtatgt ctttttggaa tattgcattc aaaggcggat taatatgggc
    17881 tgtctggcat tatccattac taattattat gtacttacct gcaggtatcg gtgttttaat
    17941 tccttctctt ataggtttta cggcttcaat aattgcaatg aattacatta ccaattttct
    18001 atatgagaaa atgaacaata tatgggcaat ggttttgctt catgctctta ataatactat
    18061 gagctttata gttgtgttgt tgtttcccaa aactccattt acaatacttt caagtatcat
    18121 ggcatgggta atagtctgga ggatagaaaa aaaatatcct cgtatcctta ctgaagataa
    18181 gattgtataa cagcgaaata caaactatta aaaaaatgaa agatttcttg atagtgctct
    18241 agaatattta ttattttatg gtataaagat tatgtctgtt atgaatttta cccataagaa
    18301 aggaacaaga tgaataaaat aaccagatac ttatatcctt taggcatgat tgctgtaata
    18361 ctgtattttt cacatacgat tttaggaaat cttttatgga aagaatataa tccagttact
    18421 acagatatca gttcattaac tgcagatgga gcccccaatg cacagctatt gagagtaata
    18481 actcttttct acagcatctt tattatactg atggttgtcg gtttggtaat aaaggctttt
    18541 aaaagctata acaggttatt gaaagctggt tttataattc ttttagtaat gcaacttact
    18601 tcagcaatag gatacagcct ttttccactt acaggtgata aaactgatat gagttttcag
    18661 aacatgatgc atataatagt tacagtcatt gtcgtattta gtacaataac attctcattc
    18721 ctagtagcat ttgggtacat taagcaggaa cacaccaaaa aacttggtaa gataactttg
    18781 gtttttgcta tttttgttac tctgtttggt ttgtttaatc caatatcaat gggtatgcag
    18841 ttgaatatat taggccttac tgaaagactt gttatttaca ctatacaggc attcatgttt
    18901 atattatcct attattacac tttcatagat aaagaaccgg taacttaata atgcttgaga
    18961 tttattctgt ttgatgttta ttgttttact gtttaatcaa aatctttagc ttcatattta
    19021 atagcggcta attctttctt taatttatca acaacatcct tatagtgctc gtcaaagtaa
    19081 acgctgtgca tttcctgagg atctttttca aggtcgaaaa gttcccactc tatatctcta
    19141 agcggcttca ttccctcctc tgagctttct cctgctgggt aataacatat cagcttgtgt
    19201 ttctttgttc ttattccata atgaggataa acattatgca tagagggaaa atcatagtat
    19261 ctgtagtaca ttgactttct ccagccatca acgttttcac ctgcagctat ctttttaaaa
    19321 cttcttccct gcatgacagg atctgcatca agttgagcaa aatctataaa ggtctgagca
    19381 aaatccagat taagcacaat ctcatcactt ctgctgccag gcttaatcat cctcggatat
    19441 ctcataacaa atggcattct taatgattct tcatacatta atcttttatc gaaccatccg
    19501 tggtctccaa ggaaaaaacc ctgatctgat gtatatacta caatggtatc ctcataaagg
    19561 ttattgtctt caagataatc taggattctt cctacattgt catcaattga tgctatacat
    19621 ttaagataat cttttatata tctttgatat ttccagcttt ttagttgtgc gtcagacaat
    19681 cctgttggtt cttgttgttt aacatcataa aaattaagat tcttatccac tctcatccac
    19741 gcttcttttg cagcgcttga acgattttca taattatcat taaatgtttc tggttctggt
    19801 atatcctcat tttcatacat atctttatga gccttgtcag gtatccatgg cctgtgtggt
    19861 gctttgtgat gacacatcat cataaaaggt ttggttttat ctctgttttc cagccagtta
    19921 attgataaat cagttattat gtctgtaaca tatcctgata actttttctt ttcacccatc
    19981 tctatcataa ctggatcgaa gtattcaccc tgaccaggaa gaacattcca ataatcaaaa
    20041 cctgtcgggt cgctgttacc gccatgacct aaatgccatt tgcctatcat tgcagtcgta
    20101 tatccgcctt ctgataatat cttaggaaat gtaggttgtc tgccatcaaa tggtatatta
    20161 agttttgtga cgccattaat atgagtgtgg gttccggata ttattgtagc tctgcttggt
    20221 gcgcaaatgg aatttgtgca gaaacagtta tcaaaaatca taccctcatt agcaattctg
    20281 tcgatattag gggttttatt aattctgctt ttgtatgcgc ttatagcatg ggctgcatga
    20341 tcatctgaca ttatgaacag catatttggt cttttcatat tgtacccttc taacatattt
    20401 tctactttta gtatatataa tcgatattgc tatatcaagt caactggctg tattaattga
    20461 aaaattagtt catgcataca aattagtata ttatcctaaa taaaatagta tataattaag
    20521 ttactaggaa ctatattaag catcaaaaaa tgcttgaaaa ctggagggtt atataatgca
    20581 ggattttgaa aagctcggag tattttattt aggtaaaaaa tatgatatcg ataacaataa
    20641 actaaaggaa gactatgttc ttctaaaatc caaggattta acaacacatg ctgtcatcat
    20701 aggaatgaca ggaagcggta aaacagggct tggaattgga ataattgagg aagctatgat
    20761 ggataatata ccagttatag ctatcgatcc aaaaggcgac cttacaaatc tggcacttac
    20821 attcccgctt ttaagaggag aggattttct cccgtggatc aatctttcag aggcttcaaa
    20881 taaagggatg gctccagaag aatatgcaaa agctcaggcc gattcctgga aaaaaggatt
    20941 agctgaatgg aatcaggatg gtttaagaat tcaaggttta aagaatgaat cagatgtgaa
    21001 aatatacaca ccaggcggtt cttcaggaat aggtgtatca gtgctcaaat catttaatgc
    21061 accttcaaag gaagttttag ctgataaaga tacatacaca gacagaatca gcgctactac
    21121 cacctccctt ctatcattac ttggtataaa tgctgattca tttactagca gagaattcat
    21181 actagtatca aaaatactgg acaacagctg gacagaagga aaaagccttt cccttccaca
    21241 gcttatagct gaaattcaga atcctccatt tgacaagatt ggggtaatgg acataaacag
    21301 tatatatcct ttaaaagacc gctttacgct tgctatgcta cttaataacc taatagccca
    21361 tcctggtttt gaagcctggc ttgaaggaga acctttaaat attgacaaat tcctatatgg
    21421 aaatactgga aaaccaagag tatccgtttt ctcaatatct catctttctg atagcgaaag
    21481 aatgtttttt gttactatgc ttttaaatga agtgctttct tgggcaagaa gccagcctgg
    21541 cacgggcagt ttaagggcaa tgatatacat ggatgaatta ttcggatatc taccacctgt
    21601 ttcaaatccc ccttcaaaag cacctttact tactctttta aaacaggcac gtgcatacgg
    21661 attaggttta gtgctatcaa cacaaaatcc tgttgacctt gactacaagg cattatctaa
    21721 tgcagggact tggtttattg gaaggcttca gacagaacgt gataaacaaa gagtaataga
    21781 aggacttgaa ggtgctgcag caggaggttc ttttgataaa aaccgtactg agcagatact
    21841 tgcgggaatt ggaaaacgag tattttatct gcacagcgta catgaagatg aaccagctat
    21901 atttaatact cgttgggtaa tgtcatatct agcaggacca ttaacaaaaa atcagatatc
    21961 tttacttcca tcttctaata ttacttcaga catccctata accacagatc aatttaaaca
    22021 gcaggaacaa ttaaatactc aaattgatga gactgtttcc acatcagtag tactttcacc
    22081 gtccattaag caggtttatc ttccagagaa gataagatca agaggaaaat tagtatataa
    22141 accagcagtt atcggtgtag cagatgtttt gtataacaat gagaagcaca tgattaccgc
    22201 aacaaaaaat tatacgattt taactcagtt caatgatagc cctgtaccgc tggactggaa
    22261 tcagtcacaa atcattgata tggatattaa cgatatcgag acaactgcaa tagaaggagc
    22321 tttgtttgct aattatcctt cttctgctgc aaatgcaaag aattatgata cctggaagaa
    22381 gttattgtca cagcatataa gaacaaatct ttcgctaaaa ctgtattcat ctccaaaatt
    22441 gaaaatggtt tcggaatatg gtgaagaaga aagagacttt ttaataagag tacagcattc
    22501 tgctcatgag aaaagagatt tagaaattga atccatgaaa aagaaataca gctcaaaaat
    22561 ccagactttg gaaaatgctc taatcagagc aagacaggct gttgaaaaac agaatgcaat
    22621 ggcatcgcag aagaaaatgg atgcaatggt ttctgccggt agtgcgatat tgggagccct
    22681 atttggcaga aaaacaatta gttcaacttc agtttcaaag ataggaacag cagtcagcag
    22741 gacttcaaag gctatgaaga gcggacagaa tatagaccag gctgaagaaa gacttcaagc
    22801 acttgaggtt cagtatgaaa acatacaaat agaattagag cagaagctta aggatgtatc
    22861 ttcagaatat gatttaataa aaaatcatat tgaaacaatc aatataagag ctacaagcac
    22921 gaatataact atacatctgt taggtcttgc atgggttcct tatgaaaaag gagacattaa
    22981 agacactatt cttatttaag cttaaaacat atcagacaaa tattaaataa attattttca
    23041 tacttgacat cattatataa atatatgatg tcattttatt ttgttttatt atttaattac
    23101 acaataaaat agtcaaggca tataattaac tgaacagtca cacatattaa acagttacac
    23161 caaaaggaga ttttaaatat gggtaagaaa gtagtcctgt tagttttaga tttgtttgca
    23221 gattgggaag gagcatatat atccacaatg cttcaagata acaacatcag tataaaaagc
    23281 caggttatgt gggcttctat tgataaaaaa acaaagaagt cgatgggtta catgacaatg
    23341 attcccgata tcacactaga agaaattcct gatgatatag atgcattgat tcttatagga
    23401 ggtaaatcat acagatcaaa tgatgctgat aaactgacat tttacatcga taaatttata
    23461 aaagctggaa aggttgtcgg ttttatatgt gatgcaacat attatgcagc taaacaaggg
    23521 tttcttaaca atgtaaaaca tactggaaat ggtttgcatg aaatgattaa gctgagagaa
    23581 tataccaatt cagataatta cataaatgaa gatgctgtca gcgataacaa cattataact
    23641 gcaaatggta ccagccctgt ggtatttgca tcactaatat tgaaagcatt gaactttctg
    23701 cctgaaaaag aaattgaatt catgaccgac atgcatctaa taggatacat gaatgctttg
    23761 ataaagcatg ggtacataaa ttagatttta caatgtgcca ggtttatcgt taattattta
    23821 atgattatca ttaaattttt attgacatac ataaatctgc gtgctacaat ccttctgtaa
    23881 tcattttttt aatggaggat tgaaatgaaa gtaccaaatt tagtaaaata cagcaaattg
    23941 tcaaaaagca cttatggaat tattaatgta ttgttttgga tactatttgc tctaaccatt
    24001 cttgtgattc ttttaactat tgcaacgaga gttttcccga atattataaa cctgtcagat
    24061 tcggatggtt cttttcgtat aagcccggat aatataatcc aatttgaaat cagcacaggc
    24121 gcaatctcaa acacaagaat agtagaaatt tattcgctta tgtttgcagg cagtgctcta
    24181 ttactcatac taatcttatt agttttaagc cagttaaaaa ttattcttaa aaatgttgta
    24241 aataaaaacc ctttcgcaga aaaaaatgcg aaaagtataa agaatatcgg ttatgtgatt
    24301 atggctagtt cattagtaat gccagttacg aaagcatttt tgattaataa aatcatacat
    24361 gcttttaatt taagtaatgt aaatgtagtt tacacagtaa atatcgaagt actaattata
    24421 ggaatactgt tactcttagt aagtgcaata tttaattatg gcgcatactt acaacaggaa
    24481 tatgatacga ctctatagaa tcgaggttaa gatatggcta ttattataag acttgacaga
    24541 gttttagcag acagaaaaat gcagcttaac gaactagctg acagtgttgg aatttcaata
    24601 gtaaatcttt caaatctcaa gacgggtaaa gttaaggcta tacgcttttc caccctaaat
    24661 gaaatatgta aaatactaaa gtgcactcct ggagatatac ttgaatatga atatacagaa
    24721 aatgaatctt caccagagga agattaaaca gtcctatgaa gattcatttt aagtaaatct
    24781 agaataattt ctttatcttt ccaaaaagac catcagcttt gtccaaatta agttttgctt
    24841 taacaccatc aatgagcttg ttaacctgat catctggaag gtcgacacct agtacagatt
    24901 caacagcttt tactggatca tccttgaact ttttcccaaa ttcatcatct gccttgattt
    24961 tttttactat ttcatcaatt tttttcttca agtccattat cgctctcctt
//
LOCUS       DAOSXM010000026        24522 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2111
            NODE_1309_length_24522_cov_9.836065, whole genome shotgun sequence.
ACCESSION   DAOSXM010000026 DAOSXM010000000
VERSION     DAOSXM010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864178
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 24522)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24522)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:24:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,261
            CDSs (total)                      :: 2,229
            Genes (coding)                    :: 2,218
            CDSs (with protein)               :: 2,218
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 3 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24522
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1309_length_24522_cov_9.836065"
                     /isolate="AMR_MDS_2111"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            172..1500
                     /locus_tag="PLQ68_05245"
     CDS             172..1500
                     /locus_tag="PLQ68_05245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HXK72088.1"
                     /translation="MVTITRMEPIDKGLNFEYCHDSENTLLMTIRSISPYTPVIEKKL
                     ENKNGKITIGDLENNKDYYISLSLQDDFGRITEPTCTRLFTPFKNKKNYKIVNYIHKN
                     DFYYYPSGRSTCSPSIIKLENGQYICSHDVYWGHASQNLTIVLKSEDKGITWNYLSQI
                     SPCFWGSLFVHKNILYILGATTEYGDIVIHKSNDYGKTWSESTILFTKRKDEEQGYHK
                     APMPVITSNNRLWTSIEHGTWKHKFSTHIVSADIDSDLMNKNSWTITDPLNIHKDAKG
                     IIAKEDPVCIEGNSIKGFDNKIYNLLRCNLTENRCAVLCQADADNPSAPLKYIEAVDL
                     PISHSKFVVRNGNDGYYYAMGNRIDNRCKNQRQILSMYKSKNLIDWHFCEDILDLLST
                     ADRKKAAMQYPDWFIDENDIVCVSRTAMNKPQNFHNANNITFHRIKDFFK"
     gene            complement(1510..3222)
                     /locus_tag="PLQ68_05250"
     CDS             complement(1510..3222)
                     /locus_tag="PLQ68_05250"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HXK72089.1"
                     /translation="MNTKIKAPWIPSYKNIPFNLEYPDISMFEMVERSAKKNPDLYAY
                     DFLGVKCTYRNFVLKVHLCAKALKSIGIKEGDRVTVCLPNCPQALFMFYGLNLIGALG
                     NMIHPLSSEGEIEFYLKDSNSRAAITLDQFYPKFEAIRERVNLEFLIISSIKDELNIP
                     LAIGYELTQGRKIKKLPKDAAVIWYKDFMKKGEIYTGSYIVKRKGNDPAVILYSGGTT
                     GTTKGILLSNLNFNALAMQTMTAGDCIVEGHAMLSIMPVFHGFGLGVCIHTILCASCK
                     CILVPKFNALSYAKLLKKKQPNYIAGVPTLFEALLRNPYMDKVNLSCLEGVFSGGDSL
                     SIELKRKLDKFLKDHGAKVQVREGYGTTECVTASCLTPKDFYKEGSIGIPYPDTYYKI
                     VKVGTTDEVPYNEEGEICLLGPSVMLGYCNNEKETKDTLKTHDDGYVWLHTGDLGKMD
                     EDGFIYFCQRIKRMIITSGYNVYPSQIENIIDAHEAVSMSCVIGVKDPYKVQKVKAFV
                     VLKAGYEQNEQTRQSILDHCKKNIAKYAMPYELEFRNDMPKTLVGKVAYRELEKEELE
                     KQQA"
     gene            complement(3206..4096)
                     /locus_tag="PLQ68_05255"
     CDS             complement(3206..4096)
                     /locus_tag="PLQ68_05255"
                     /inference="COORDINATES: protein motif:HMM:NF012424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HXK72090.1"
                     /translation="MVGSEYKRRLGDRKDARRIRNDEMDAVHSLFPFIAPKRTEAEVY
                     IKQEMDVTELVSYVKKIKENNEDCKMTYFHAFVTAIAKTVYMRPLLNRYVMAKKYYER
                     NDIILSFIAKKKFNDTAEEMLVSLKVRKDENLDDISKFISSDVKKIRQQGTNSIDGTL
                     KILQKLPRFVNCIFIWLMKVLIYFDLYPAEFQKGDTNYSSVLISNLGSIKCDAPYHHL
                     NNFGTNSVVICIGEIHKKEVISADGEKRLRDVVNFGITLDERIADGFYFAKSIKFLQY
                     IMANPELLERSVGDEIEYKD"
     gene            complement(4178..4732)
                     /locus_tag="PLQ68_05260"
     CDS             complement(4178..4732)
                     /locus_tag="PLQ68_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HXK72091.1"
                     /translation="MSYIPSPKEAMQILKEYNKDDFHILHANTVGEVLKHFASIYDKD
                     NQEYWQAVGILHDIDYELYPKEHCVKVEQLLKTRNIDENMIRSIMSHGYGICSDIKPV
                     SFMEKILYTVDELTGLIWATALMRPSKSVMDLEPKSVIKKFKQLSFAKAINRQTILDG
                     LEMLDMEMNELISMTIDGMRKMEQ"
     gene            complement(4729..5916)
                     /locus_tag="PLQ68_05265"
     CDS             complement(4729..5916)
                     /locus_tag="PLQ68_05265"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXK72092.1"
                     /translation="MRIGQFTDSFLPIVDGVGRVVYGYADALCARGHECYVITPIADA
                     GFRGKYSFDIIDFYGVTIPEYTQYKMGIPLWDVHYHERIKTVKFDIVHVHSPFRTGYE
                     GLRIAKKHKIPVVGTFHSKYYEDFLQVLKIQPLAEAGVKIVADFYEKCDMVWAVSENS
                     AEALRSYGFKGKIETMRNGTQIRKPEKKNIDYVKQMYNLGSVPVMLYVGQMNWKKNIK
                     KILEATAILKRTGFNTKLILAGQGPHAEEIKRTVSELNLNDSVEMTGHVADIYVLDGL
                     YGASDLFVFPSLYDNSPMVVREAAAMMTPSVLIRNSSAAEGITDGYNGFLCSDDSNDL
                     ANTLKRALFDEKNKLHEIGKNAQSAIPVSWDSIMVDVEDRYKELITVNKKGSKQKRKK
                     ILI"
     gene            complement(5922..6236)
                     /locus_tag="PLQ68_05270"
     CDS             complement(5922..6236)
                     /locus_tag="PLQ68_05270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72093.1"
                     /translation="MNTLNDIIKQYKVQLLETKKKYYEQLKALPKGTLSVKKMGSGEY
                     WYLKYREGKRIVTKYIGKLSEKVVEIEKQIALRKTLEIMLKELETELALIKKIETIAQ
                     GA"
     gene            complement(6305..7516)
                     /locus_tag="PLQ68_05275"
     CDS             complement(6305..7516)
                     /locus_tag="PLQ68_05275"
                     /inference="COORDINATES: protein motif:HMM:NF018877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AGE family epimerase/isomerase"
                     /protein_id="HXK72094.1"
                     /translation="MNNLEKYRQLIRSHLLEDVMQFWQNSDLIDKVNGGYYTSVDRFG
                     KLYNTDKSVWFQGRCLWTYSALINKYGYNPIWVENAKTGKAFLEKYCTDTDERMFFTV
                     TKDGKPLRKRRYFFSESFYCIAMAEYGNAMKDKESLKKAEKCFEMMNMIYNNPDSDTY
                     KITPKSYPTRKERSAANAMVMLSTSQILRRCIPQKAEYYSRIVKYICNDIIKYHYKED
                     MKCVLETVSDDGSFIDNPTGRTVNPGHSCENAWFLMNEAIYSDNSDLMQKALNILDWS
                     LELGWDEKFGGIYYFRDIYNKPSDQLEWDMKLWWVHNEAMIACLTAYGITKQQKYFDM
                     FERILQYCLQNFHDKQYGEWYGYLHRDNTVSHTQKGTLWKGPFHLPRCLMICDTLLGN
                     IENGESIKPFL"
     gene            7812..8894
                     /locus_tag="PLQ68_05280"
     CDS             7812..8894
                     /locus_tag="PLQ68_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008780899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD/NADP octopine/nopaline dehydrogenase family
                     protein"
                     /protein_id="HXK72095.1"
                     /translation="MKITIIGTGNGGSAMAADLSIKGHEVTLFKSSDKGNNEHFDMLV
                     EQNGEIEFNDIDGSKKVKIHKVTNSISEAFSNNPKAIVLFIKTNYHEDIIKRIAPYLK
                     NDQMILMEPGYLSTAYFLKHCKNLKLILAEAQSSPLDCRVTKPGTVKILFKNERNPVG
                     IYPAKRKTEAENLLKEFDYNFIMIDSVVEAALHNPNLIVHTIGAIMSIPRIEYTDGEY
                     SMYREVFTPSIWNLVEKLDAEKMAILDSLNLAKIPYVEACKFRNSKDLSLDAKEVFYD
                     YAKNDSPTGPTISNSRYITEDVPEGLVLLESLGQLLNIETPVCTALIEIASACLETDF
                     RKDGRTTLRLGIENITALIKNKNNIN"
     gene            9047..10414
                     /locus_tag="PLQ68_05285"
     CDS             9047..10414
                     /locus_tag="PLQ68_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013656786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HXK72096.1"
                     /translation="MKFEDILATHDEKISKLSEKAYTSDLISNELYEKYNVFRGLRDN
                     KGVGVVTGLTEISEMNGYEVDKSGQRIPIEGVMYYRGINIYDIISGIKKGNRFGYEEC
                     AYLLLCGSLPNKKELEDFVCLLNDLRSVPDSFVRDVILKAPSHDIMNALSRSILTLYS
                     YDTNPDDISLKNVFRQALQLIAVMPMLAVYSYQAYRHYILGKNLHIRQPKPELSTAEN
                     FLYMLKGTGKYTKEQAHILDICMILQAEHGGGNNSSFTTHVVTSSGTDTYSCISASLG
                     SLKGPRHGGANLKVVHMMDDILKHVRNLTDSNIEKYLEKMLNKEVYDKSGLIYGLGHA
                     VYSLSDPRANILREQLDEYMSDKKRDRLYTLYDKLQCIAPKVLQKHKNITGKVAINVD
                     YYSGYLYKKLEIPEQLYTPIFAVSRIIGWSAHRLEELSNNGKIIRPAYIAVAPRREYI
                     DINKR"
     gene            10466..11359
                     /locus_tag="PLQ68_05290"
     CDS             10466..11359
                     /locus_tag="PLQ68_05290"
                     /inference="COORDINATES: protein motif:HMM:NF015509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HXK72097.1"
                     /translation="MVIVLFIIMLIGVLLGKMKVINSESRSTISFLIINVSMPMLIVS
                     SSNLEFNEQTKNESMWLIIMSVAAFFFSILLAQLIFKKAVPRCATVFSNAGYMGLPVL
                     NSILGPKGLFLGAIFQMVFHLFTWTYGISLFTKSNKFKDSLKKMINPSLIAVAIAIVL
                     MFTGWKIPDTIQTVLKMMGELTTPLSMLLIGATLAQVSLKEVIKLKQIYIVTALRLLI
                     IPAFVLLFLLFPSIPKISVYVVFVINAMPSAAITGILAMKYNNDEKLASSIVAFSTLI
                     SIITLFLITNVFDVASVLGLV"
     gene            11539..12888
                     /locus_tag="PLQ68_05295"
     CDS             11539..12888
                     /locus_tag="PLQ68_05295"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HXK72098.1"
                     /translation="MRKGKTFKSLTLLLALCMIVASFVGCSPKKSNTPLTIWVGVESV
                     DFYTEKMAEYVEKYKEQTGEDFPFSVSVQGVDTASAAAKVLDDLDAGADIFTVAHDNL
                     GKLTAGASAIAPVRSEALLAQINADNPETFLNVIDMTVQGTKYTFGIPYIAQSLVLMY
                     NTKYLSENDVKTWEGIWAAAKNNNKQSVSLMGADGFNNSFLTLAINEETGEMPAKIYQ
                     NGDIEDCEFTSDETVAIMQWGQRFFTDKNGGKMPTDSGWEIELRDEISLSLIGGAWKF
                     NAAKAALGENLGIAALPSFTLTANDVKGTNVNAGTVYRSGSFTDCKIFVMKKGSDKAD
                     YLEDILLFLSSKEVQEQSFIECANLPAYKNAITEFTSMQEDNIETILAAKQLEMFNYG
                     IPQPFGYSTTHNFYYYSKGAPDLVREILENKESLFGTTEAIRTQLQTIENIWKTGER"
     gene            12917..14317
                     /locus_tag="PLQ68_05300"
     CDS             12917..14317
                     /locus_tag="PLQ68_05300"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HXK72099.1"
                     /translation="MDNNKTGINRFLTKISKPFVRFYNRFMDGSLGTKMSHFVMGSGN
                     VYHGQIIKGLIYFIIQAGYALFMITCPEVNKTPLGYKAFINLITLGTQEGDIFTPADN
                     SMLMLLFGVVTIGITLMYLFAYNSGIKSSYKADQDIKNYGKVTSFKQDAKEMLGNRFY
                     ITMLTPAAIGAVVFTLLPTIFMILIAFTNYDKERQAGLKLFDWVGFDNFTTFFTNPTG
                     EIAARFLPVLAWTLIWAFFATFLNYFGGIVLALIINKKGLKGKKIFRAVFVLTIAIPQ
                     FVSLLAMRNLIGEYGPLQSFLVQTGILSEPMSFLANATNVWTARITIILINLWVGIPY
                     SMLLASGAIMNIPDSLYEAARIDGAKKFTILRKITMPYIVFITTPYLISSFVGNITSF
                     NIIYLLTSGNPKVTGGYIAGGTDLLVTWLYKLTIDEQQYNLGAVIGILTFLITSIVTL
                     VTYRRSKSYKEEDTFQ"
     gene            14314..15249
                     /locus_tag="PLQ68_05305"
     CDS             14314..15249
                     /locus_tag="PLQ68_05305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013657949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HXK72100.1"
                     /translation="MSANTKTITIDKKDGNKPVKEKFFNQTRSEKVSKIGTYILLIAL
                     SIIWLYPILWILLNALRTEFNVDGTMVGTVVSHYWPKQFGFDNFKILFTNTYFPIWFR
                     NTLFVAVFSCVLSTMLTLSVAYVMSKVKFKMRKPFMNIAIVMGLFPGFMSMIAIYYIL
                     KALGLTQTLTALILCYSAGAGLGFYVGKGFFDTVPNALIEAARIDGAGEARIFGSVIL
                     PISKPIIVYTALMSFTAPWMDFIFARVILGEQNTELHTVAVGLYYMMYGQIVDTSRFT
                     VFAAGCVCVAIPIVALFMYLQKYYIEGSTAGSVKG"
     gene            15249..16859
                     /locus_tag="PLQ68_05310"
     CDS             15249..16859
                     /locus_tag="PLQ68_05310"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HXK72101.1"
                     /translation="MKTITKKILSILVSILMMLTFASCSTDALRGEKAIKIDELPDID
                     VPGVYYEIFVGGFSDSDEDGTGDIKGIINRIDYLNDGDPLSGKSLGIEGIWLMPIMQA
                     GSYHKYDVVDYYSIDLNYGTMEDFEQLISVCDAKGIDIIIDLVLNHTSEYNRWFLDLK
                     NAIKEGDKDNKYMDWYSITTDSSKGWHKLAEDPDGVQYYYEGNFSPSMPELNMDNPEV
                     LDEIDNIVKFWLEKGVKGFRLDAVKYVYLSNDIKNIEFWKWFADTCRKYDEDVFLVGE
                     NWSNTTSVYEYYEALDCFDFDMAGSGGDVALTVNGIFNLNDYVRNICRYKENVEISNP
                     DAILKPFISNHDMDRSAGYLSVKENRMHMAANLYMLSSGNPFMYYGEEIGMYGSRATS
                     STDANRRLAMLWGDKDTVQDPPGTTFPAERQINGTVKQQIKQHDSLYTYYKKLIALRK
                     ANPEIAVGDYIPLKFEGYNLFGGFLVQYENVTKGVFHNLSNEPITIDLSTYTDIKFDS
                     ISGYIGQGKARLNGNILTLDGLTSVILK"
     gene            16997..17581
                     /locus_tag="PLQ68_05315"
     CDS             16997..17581
                     /locus_tag="PLQ68_05315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72102.1"
                     /translation="MKKFKVIAIVLTLVLALGSLAACTPDTILENTEKDYYVTGQFAG
                     WGDAVGNDTYKMTPVSLKDARVAALKADLKGAKYLYILENVTITAEGAGWSAQYVENG
                     AVKEADGNQTMKWLQVSKGQEAPDWWAQSPESGEIVSLTPDLLWIPGFTETPEVGPDW
                     NGNPVVLKAGTYTVVFAIVEKEEGLVKVAGLIAE"
     gene            complement(17724..20051)
                     /locus_tag="PLQ68_05320"
     CDS             complement(17724..20051)
                     /locus_tag="PLQ68_05320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72103.1"
                     /translation="MNLLLCELKKTFVSKTTVIFLIIAVTINVITLYINESVSSGSSL
                     DLSIVRDIDEKLMHLDENQAKDFLNEHIIAMEYALSLTSEIPLTPEKLVLRYPGNNIE
                     KIIADYNNGVYDGYIKNPYAIGIVLRQKLEEVDVVYSYDEYREEINRIAIRMQTSPIF
                     ADSHFAKHNAKKTAEAFSNLNISNLRFNSSKGVTLATKNGITPIYLLVACLFICFLVY
                     IHDKDNNTLTLLRTMRKGRKHLMLSKIISALILGLVTTFLLSVSSYLTGYVLYGFGDI
                     NMPLQSVFGFQSCIFELSVLQYLIVYIPMLMLSVIFVLTLMMFIFVSIKDSIVGVIIV
                     ASIFTISIVMHNSISSVSFFSIFKYLNLYFLLDAGKIISNYLNINMAGYPLSFVPIFF
                     ITVITSSVILSLVTINIFKGQKDIQGSYLKRNIRKIKTSKAKKINTGIFCFEAYKCII
                     INPVLPILIIFLLFQLLTVKAYPPEQRLATKDYIYMYYMKKLEGPISNEKTLTLEKML
                     KEVQTENNQDASSLNRDKISVILNLISKDSSLNLLKDSTEDINIKNRISFVYEKGYNE
                     FFNDDYLSRKAALYSLLLVILCTVDIFAMEKKNNTYSIITSTVHGRKRTFINKSIISC
                     IIAFLVMSAAYIPLIIKIARESGFACFDSPIACINGMENFPLNVSISEYFILLFILKF
                     IGIVISISIINFVSHITGERIMTIAITLPVIVIPAFLAFMGADSFSNILFNVYLYGNN
                     LMSLGQWCVVQAAALVVIGLLLFYILYMKYNKNRI"
     gene            complement(20048..20950)
                     /locus_tag="PLQ68_05325"
     CDS             complement(20048..20950)
                     /locus_tag="PLQ68_05325"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXK72104.1"
                     /translation="MELMLENINKSYGLIKALNGFTAVLDDGVYGLLGPNGAGKSTLI
                     KIITDSLKSDSGSVYINGKSIKTIGDSYRSMLGYMPQHHGIYPSFTAAQFLSYIGSLK
                     AMDKKRIEDEVPKILKSVNLLDVAHRRIGTFSGGMKQRIMLAQALMGDPKILILDEPT
                     AGLDPKERIRMRNLISGYALNRIVIIATHVVSDIEYIAKKVMLIKKGVLINYDYPYEL
                     CKKLEDKVYEIEASEVELLDIILSFNVCNIQKSVDNLIVRILLHEHQTESDLSKYSHR
                     QTRATIEDVYLSVFEEERETEVMQ"
     gene            complement(20966..22156)
                     /locus_tag="PLQ68_05330"
     CDS             complement(20966..22156)
                     /locus_tag="PLQ68_05330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72105.1"
                     /translation="MNTNKRIYLLIILIAVILTFLCGCNKKQSNNNDTAVTYEGSAYG
                     ENGMIIYEDGLLAYYDYKTGERSVVCSRPECSHQKYDSQTNPNPSCAAVPPKNTTFEA
                     GVIVNNYLYMFVLPSEINKMDIYKSDLDGENRIKAGQVNCGGIIDKAIVYKNLFVFTG
                     KELVKKDNEDELSVSTIQIKYFTGIIDINTLELVQIETYEKHQISQSYYVYNNVLHYY
                     YMDENVYEIRRVSLSDFTVLEALSLPLKNEQEKAYCVMDKGYLYYIKSNPMQSSPDIR
                     RTDLMTLEDEIFVSGIEIQDGTLIGISGKYFFIHSNESKIPEETEWKFVIYDSDEKTI
                     NECDMHYSGVQTGLVTHLINLNEEFILLYKFDEGSKHASYRYMKIESLINKSYDFVDI
                     NVIY"
     gene            complement(22172..23761)
                     /locus_tag="PLQ68_05335"
     CDS             complement(22172..23761)
                     /locus_tag="PLQ68_05335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72106.1"
                     /translation="MDYQAMWPEKKMLVWLYFDYFGILQYINNLDNSNPKPFSEFYTS
                     IVSDELIVAYNDYLISLGKDYVIRFIRQDAIVDKIAEDYKALGDDNNAKQYFKFTEIY
                     AYYFEFMKDMKNSGIQADIISSIANIKGTFSIKENDVTVQVNYTAIEESGADEINNDL
                     LLDITDFLLDDEDKKLYGLFPDEYWSLFDKYNGIYGVYNNEIASSFIFYLRNDIINKY
                     EVNISELDTIDEIDVFFDEIFDDYKTEITSSDLMLIRKFLDYSAYNELAGIITDKSNS
                     TISYKYDADNNPAVINRFKDADYLQINKILAKWAARGYFNTSIVYNTDGSASASIAFA
                     PYISYIVPGYQDYSNDSSDDIQYIPVEISRYILHDSRNKLIGIASWTKNGESAFDFLS
                     TLFTDPHLSNLIYYGKNYKCENGLVKQNGNYSTSVLTASHMLYPNRVGEKRISDFKDA
                     MSDAYIPPNYGFEPDITKMEYEFEEISRLCKEADSLWATHSDNFLSELENVITQLDAL
                     GYSRFLQDINKQLGQYIESKP"
     gene            complement(23974..24375)
                     /locus_tag="PLQ68_05340"
     CDS             complement(23974..24375)
                     /locus_tag="PLQ68_05340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72107.1"
                     /translation="MKNKKLFSILLFFILLFTALSVFAGSSSFSEPLGSGYWSGSLSV
                     GNLVVTAEKNLDPIPPTINLSVGVSGTVWFVDYYGNEYSSNYSEYQDYVDGVAAVLIL
                     PSGARTFTRADSYHYGYINGSCDNNYLSASN"
BASE COUNT         8090 a         4399 c         3911 g         8122 t
ORIGIN      
        1 actccttttg aagtttttat gagctagtca taatttactt aagtatatca tcatacatag
       61 caattgcaac cttttttaaa tacatattca tacgttatta attttgatat aatatataaa
      121 gtgcttaaac tttaaaacaa aatgaactct aaatacaaga aggagcatga aatggtcaca
      181 atcacaagaa tggaaccaat cgacaaagga ctgaattttg aatattgcca cgattcagaa
      241 aatacactgc ttatgacaat acgatcaata tcaccctata cgcctgtaat agaaaaaaag
      301 ctggaaaaca aaaatggcaa aattacaatt ggtgatctgg aaaataataa agattactat
      361 atcagcttgt ctttacaaga tgattttgga agaattacag agcctacatg cactagatta
      421 tttactcctt ttaaaaataa gaaaaattat aaaatagtca attatattca taaaaatgat
      481 ttctattact atccttctgg aagatctaca tgttctccca gcattattaa attggaaaat
      541 ggtcaataca tatgctcaca tgatgtgtac tggggtcatg cttctcaaaa ccttacaata
      601 gtattaaaat cagaagataa aggtataaca tggaactatc tgagccaaat atctccctgc
      661 ttttggggtt ctttatttgt acataaaaat atactttata ttttaggtgc aacaacagaa
      721 tatggagata tagttattca taagtcaaat gattatggaa aaacatggtc tgaatccaca
      781 atacttttta ccaagcgcaa agatgaggag caaggttatc ataaagcacc catgcctgta
      841 attacatcta ataacagact atggacaagc atagagcatg gaacatggaa gcacaaattc
      901 tcaactcata ttgtctctgc tgatattgat agtgatttaa tgaataaaaa cagctggaca
      961 attacagacc cgttgaatat acataaagat gcaaaaggaa ttattgcaaa agaggaccct
     1021 gtttgtatcg aaggaaattc aataaaagga tttgataaca aaatatacaa cctgttaagg
     1081 tgtaacctta cagaaaacag gtgtgctgtt ctgtgtcagg ctgatgctga taatccgtct
     1141 gctccattaa agtatattga agctgttgat cttcctatct cacatagtaa gtttgtcgta
     1201 agaaacggta atgacggtta ctattacgca atgggaaaca gaattgataa tcgatgcaag
     1261 aaccaaagac agatattgag tatgtacaaa tcaaaaaatc tgattgattg gcatttttgt
     1321 gaggatattc ttgatcttct ctctactgca gacaggaaaa aagcagctat gcaataccca
     1381 gactggttca ttgatgaaaa tgatatagta tgtgtaagca gaactgctat gaataaaccg
     1441 caaaacttcc ataatgcgaa caatattaca tttcacagga taaaagactt tttcaaataa
     1501 ataaggtatt tatgcctgct gtttttctaa ttcctctttt tccaactctc tgtatgccac
     1561 ttttcctaca agtgtttttg gcatatcatt tctaaattca agctcataag gcatagcata
     1621 ttttgcaata ttctttttac aatggtcaag tatactttgt cttgtctgct cattttgttc
     1681 ataacctgct ttcaaaacaa caaaagcttt tactttctgt actttgtatg gatctttaac
     1741 accaattaca caggacatag aaacggcttc atgagcatca attatattct ctatttgcga
     1801 tggatataca ttatatcctg atgtaataat cattctcttt attctctggc agaaatatat
     1861 gaaaccatct tcatccattt tacctaaatc acctgtatgg agccacacat atccatcatc
     1921 atgagtcttc aaggtgtctt tagtttcttt ttcattattg caatatccaa gcatcacaga
     1981 aggtcctaaa aggcatatct ctccttcttc attatatgga acttcatctg tagtaccgac
     2041 ttttactatc ttgtaataag tatcaggata aggaattcca atacttcctt ctttgtagaa
     2101 atctttcggt gtcagacagc ttgcagtaac acattcagta gttccatatc cttctcttac
     2161 ctgaactttc gcaccatggt ctttaagaaa tttatccagt tttctcttta attctataga
     2221 caatgaatct cctccggaga aaacgccttc aaggcagctt aagttcactt tatccatgta
     2281 tgggtttctc aataaagctt cgaacagggt tggcactcct gcaatatagt taggctgttt
     2341 tttcttaaga agttttgcat acgataatgc attgaatttt ggcacaagta tacacttaca
     2401 agaagcgcaa agaatagtat gaatgcaaac tcctaaacca aaaccgtgaa aaactggcat
     2461 tattgaaagc atagcatgac cctctacaat acaatctcct gctgtcattg tctgcatagc
     2521 aagtgcattg aaattcaagt tggacaataa tatgccttta gtagttcctg tagtaccacc
     2581 actgtagagt ataactgcag gatcattacc tttacgcttt acaatgtaac taccagtata
     2641 aatttcaccc tttttcataa aatccttata ccagataaca gcagcatctt taggaagttt
     2701 cttaattttt cttccctgag taagctcata tcctatagcc aaaggtatat tcagctcatc
     2761 cttaatagag gaaattatca aaaattccag attaaccctt tctcttatag cttcaaattt
     2821 tggatagaac tgatctaaag ttatagcggc tctgctgttg gaatctttta agtaaaactc
     2881 tatttcaccc tctgaagaaa gaggatgtat catgttgccc agtgcaccta taaggttcag
     2941 tccataaaac ataaataatg cctgaggaca gttaggaaga catacagtta ctctatcccc
     3001 ttcttttatt cctatgctct ttaatgcttt tgcacataaa tgaactttta gcacgaaatt
     3061 tctgtatgtg cattttaccc ctaagaaatc atatgcgtaa agatcgggat ttttctttgc
     3121 ggatctttcc accatttcaa acatagaaat atcaggatat tcaagattaa aaggtatatt
     3181 cttgtaactt ggaatccatg gagctttaat ctttgtattc aatttcatca cctacagacc
     3241 tttctaataa ttctggattt gccattatat attgtaaaaa ctttatactc tttgcaaagt
     3301 aaaaaccatc agctattctt tcatcaagcg ttataccaaa attcacaaca tcgcgtaatc
     3361 tcttttcacc atctgcactt ataacttctt ttttgtgtat ctctccaata catataacta
     3421 cggaatttgt accgaagttg ttcagatgat gatatggagc atcacatttt atacttccaa
     3481 gattggatat aagaacagaa gaatagttag tatctccttt ttggaattct gcaggatata
     3541 aatcaaagta aatcaagact ttcattaacc atatgaaaat acaattaaca aaccttggca
     3601 gtttctgaag aattttaaga gttccatcaa ttgaatttgt tccctgctgt ctaattttct
     3661 taacatcaga ggatataaat tttgatatat catccagatt ctcatctttt ctgactttaa
     3721 gagacaccag catctcttca gcagtatcat taaatttttt ctttgcgatg aacgaaagaa
     3781 ttatgtcatt tctttcataa tacttttttg ccatcacata tctgtttaaa agtggtctca
     3841 tatagactgt ctttgctatt gctgtgacaa aggcatggaa atatgtcatt ttacagtctt
     3901 cgttgttttc tttaatcttt ttaacataag aaacaagttc tgtaacatcc atctcctgct
     3961 ttatatatac ttcagcttct gttctttttg gagcaataaa aggaaataaa ctgtgtacag
     4021 catccatttc atcgttcctg attctgcgcg cgtcttttct atcacccaga cggcgtttgt
     4081 actcactacc aaccataaac tccccgtatc ctccttatgt ttcccaaaca taagaattgt
     4141 atatttaata gctgaagcct acccccagcc acagccatta ctgctccatt ttcctcattc
     4201 catcaatagt catggaaatt aactcattca tttccatatc cagcatttcc aatccgtcaa
     4261 gtatagtctg cctgtttatt gctttggcaa aggaaagctg cttgaatttc ttaatcactg
     4321 atttcggttc taaatccatt acgctttttg aaggtctcat aagtgcagta gcccatataa
     4381 gacctgtaag ctcatcaact gtatatagta ttttttccat aaacgaaaca ggcttgatat
     4441 cactacaaat tccatatcca tgactcatta tacttctaat catattttca tcaatgtttc
     4501 ttgttttaag taactgttct actttcacac agtgctcttt aggatacaat tcataatcta
     4561 tatcatgcag tattccgact gcctgccagt attcttgatt atctttgtca tagattgatg
     4621 caaagtgctt taatacctca cctacagtat tcgcatgaag aatatgaaaa tcatccttat
     4681 tgtattcttt taatatctgc atggcttctt tgggtgaagg tatataactc atataagtat
     4741 cttcttcctt ttctgtttgc ttcctttttt atttacagtt atcaattctt tatatctatc
     4801 ttcaacatca accattatac tatcccacga tacaggaata gcagattgcg catttttccc
     4861 tatttcatgc aatttatttt tttcgtcaaa caatgctctt ttcagggtgt ttgcaagatc
     4921 atttgaatca tcagaacaca aaaaaccgtt gtaaccatca gttattccct ctgcagcact
     4981 actgtttctt attagcactg aaggtgtcat cattgcagct gcttccctga ctaccatagg
     5041 actgttgtcg tatagcgaag gaaaaacaaa taaatctgat gccccatata atccgtcaag
     5101 cacataaata tctgctacat gacctgtcat ctcaactgaa tcatttaaat tcaattcact
     5161 gactgttctt tttatctctt ctgcatgagg accctggcct gcaagaataa gctttgtatt
     5221 aaaaccagtt cttttcagaa tagcagtcgc ttccagaatc ttcttaatat tctttttcca
     5281 gttcatctga ccaacatata gcatcactgg aacagaacca agattataca tctgttttac
     5341 ataatcaata tttttctttt caggctttct tatctgtgtt ccgtttctca ttgtttcaat
     5401 ttttccttta aagccataac tgcgtaacgc ttcagcagaa ttttcactaa ctgcccatac
     5461 catatcgcat ttctcataga agtctgcaac tatcttaact cctgcttcag ccaaaggttg
     5521 aattttcaga acctgaagaa aatcttcata atactttgaa tggaaagtac ccacaacagg
     5581 tattttatgt ttttttgcaa ttcttaaacc ctcatatcct gttctgaacg ggctatgcac
     5641 atggacaata tcaaatttaa cagttttaat tctttcatga taatgcacat cccatagagg
     5701 tatacccatc ttatactgag tatattcagg aattgtgact ccgtaaaaat caataatatc
     5761 aaacgagtat ttgcctctga aaccagcatc tgcaattgga gttattacat agcattcatg
     5821 ccctcttgca caaagggcat ctgcgtatcc atatacaact cttcctacac cgtcaacaat
     5881 aggtaaaaag gaatctgtga actgtccaat tctcatctaa attatgctcc ctgagcaata
     5941 gtctcaattt tttttatcaa agcaagttcg gtttcaagct ccttcaacat tatttctaat
     6001 gtttttctta atgcaatctg tttttctatc tctacaactt tttcagaaag ctttcctata
     6061 tacttggtaa caattctttt gccttctcta tatttcagat accaatattc gccgctaccc
     6121 atcttcttaa cggacaatgt cccttttggt agggctttaa gctgttcata atattttttc
     6181 tttgtttcaa gcaattgcac cttgtactgt ttgataatat cattcaatgt attcatgcaa
     6241 gtcattatac ccaacacatt tacaaatgtc aacataatgt tgggtaagat gtaaacaccg
     6301 caaatcacag aaacggttta attgattctc cattttctat atttcccaat aatgtatcac
     6361 aaatcatcag acacctcgga agatggaaag gccctttcca tagtgttcct ttctgggtat
     6421 gtgaaacagt attgtctcta tgaagataac cataccactc cccatactgc ttatcatgaa
     6481 aattttgtaa acagtactgt agtattcttt caaacatatc aaagtatttt tgctgctttg
     6541 ttattccata tgctgttaag catgctatca tagcctcatt atgtacccac caaagcttca
     6601 tatcccattc taactgatca cttggcttat tataaatatc tctaaagtag tatataccgc
     6661 cgaacttctc gtcccagcct aattccaaag accagtcaag aatattcagc gctttttgca
     6721 ttaaatcaga gttatctgaa tatattgctt cattcataag aaaccatgca ttttcacatg
     6781 aatgccctgg attaacagtt cttcctgtcg gattgtctat aaatgaacca tcatctgaaa
     6841 ctgtctctag tacgcatttc atatcttctt tataatgata tttaataatg tcattacata
     6901 tgtatttaac aatccgtgaa taatactcag ctttttgagg aatacacctt ctcaatatct
     6961 gagatgttga tagcattacc attgcatttg cagctgagcg ctcttttctg gtcggatatg
     7021 actttggcgt tatcttatat gtgtctgaat cgggattatt ataaatcata ttcatcattt
     7081 caaagcattt ttcagctttt tttagtgatt ctttatcttt cattgcatta ccatattcag
     7141 ccatcgctat gcaataaaag ctttctgaaa agaagtatct tctttttctt aagggctttc
     7201 catcttttgt tactgtaaaa aacatccttt catcagtatc tgtgcaatac ttttctaaaa
     7261 aagcttttcc agtttttgcg ttttctaccc atataggatt atatccgtat ttatttatca
     7321 aagctgaata tgtccataaa catctgcctt gaaaccaaac agatttatct gtgttataca
     7381 gctttccgaa tctatccact gatgtatagt aaccgccatt aactttatcg ataagatcag
     7441 agttctgcca gaattgcatt acatcctcta ataaatgtga tctgattaac tgtctgtatt
     7501 tttctagatt gttcattttg cccccttgaa taaaaaaaat tgtgattact aatatgttaa
     7561 tactatttta tcatgaactc atgaacgaag tataacttat attaatatct tctattataa
     7621 ttcatttaaa aattatcatt aagtcttaca acttcaaaat ctatatgctg tctttacatc
     7681 ttgtggttgc tagaacgtgc aaaatggata tcttatcttg aaaatgatat aatgtaataa
     7741 aagaaatagc attattgaga gagatattat acaaactctt gattttcgca tcccaaagaa
     7801 aggatagaaa catgaaaatt acaattatag gaactggtaa cggcggatca gcaatggctg
     7861 cggatttaag cataaaagga catgaggtca cattatttaa atcatctgat aagggtaaca
     7921 atgagcattt tgatatgctt gtagaacaaa atggtgaaat cgaatttaat gacatagacg
     7981 gttctaaaaa agtaaaaata cataaagtca caaattctat cagtgaagca ttttcaaata
     8041 atcccaaagc aattgtattg tttattaaaa ctaattatca cgaagatatc ataaaaagga
     8101 tcgcacctta tttaaaaaat gaccagatga tacttatgga acctggatac ttaagcacag
     8161 catatttttt aaagcattgc aaaaatctta aattaatatt agctgaagca caaagttccc
     8221 ctttggactg tcgtgttaca aagcccggta cagtaaaaat tctatttaaa aatgaacgta
     8281 atccggtagg catttatcct gccaaaagaa aaactgaagc agaaaactta ttgaaagagt
     8341 ttgattacaa tttcattatg atagattctg tcgtggaagc tgcattacat aatcccaatc
     8401 ttatagttca caccatcgga gcaataatga gtataccaag aattgaatac actgatgggg
     8461 aatattccat gtatcgtgaa gtattcaccc ccagcatctg gaatctggta gaaaagttag
     8521 atgctgaaaa aatggccatt cttgattctt taaatttagc aaaaatacct tatgtggaag
     8581 cttgtaaatt tagaaattca aaagatctca gtcttgatgc caaagaggtt ttttatgatt
     8641 atgcaaaaaa cgattcacct actggtccga caataagtaa ttcacgttat attacagaag
     8701 acgttcctga aggattggta ttattggaat cacttggaca attattgaat atagaaacac
     8761 cggtatgtac agccttaata gaaatagctt cagcctgtct tgaaacagat ttcagaaaag
     8821 atggtcgcac cacattaaga ctgggaatag aaaacattac tgcgctaatt aagaacaaaa
     8881 ataacatcaa ctaaaacatt taaatagtca tatttgtggt tatataccta actatatatt
     8941 gtgccatatt ctatctgttt aaacctcctt aaagtaaaaa atactttaaa tagtgtcatt
     9001 tttatgttat gattaaagct accaaaaaaa tggagaagtg ataaatatga agtttgaaga
     9061 tattttagct acacatgatg agaaaatatc aaagttaagt gaaaaagcat atacaagtga
     9121 tcttataagc aatgaattgt atgaaaaata taatgtgttc agaggtctgc gtgataataa
     9181 aggagtaggt gttgttaccg ggctgacaga aatttctgaa atgaatggat atgaagtaga
     9241 taaatccgga cagcgtattc ctattgaagg tgttatgtat tacagaggca taaatatata
     9301 tgacattatc agtggtataa aaaagggaaa tcgttttggc tatgaagaat gcgcatatct
     9361 tttattatgc gggagtcttc caaacaaaaa ggaacttgaa gacttcgttt gtcttctaaa
     9421 tgatttaaga tctgtacctg atagttttgt aagagatgtt atactgaaag ctccctcaca
     9481 cgatataatg aatgctttat caagaagcat acttactctt tactcatatg atacaaatcc
     9541 agatgatata tccttaaaga atgtattcag acaagcccta cagcttattg ctgttatgcc
     9601 aatgttagca gtatacagtt accaggcata cagacactat attcttggaa aaaatctgca
     9661 tatccgccag ccaaaacctg aactttcaac agcagaaaac tttttatata tgttaaaagg
     9721 aacaggaaaa tataccaaag aacaggctca tatactggat atatgcatga ttttacaggc
     9781 agaacatggt ggaggaaata actcttcatt tacaactcat gtagtaacat ccagtggaac
     9841 cgatacatat tcatgcatat cagcatcatt aggctcacta aaaggacctc gccatggcgg
     9901 agctaactta aaggtagtcc atatgatgga tgatattttg aagcatgtaa gaaaccttac
     9961 agatagtaat attgaaaaat atcttgaaaa aatgcttaat aaggaagttt atgataaatc
    10021 aggcctcata tacggcttag ggcatgctgt ttattcatta tctgatccta gagcaaacat
    10081 actaagagag cagcttgatg agtatatgtc tgataagaaa agagacaggc tttatacctt
    10141 gtatgataaa cttcagtgta tagcgccaaa agtattacag aaacataaaa atattactgg
    10201 taaagtagca attaatgtgg actactactc aggatatttg tataagaagc tggaaatacc
    10261 tgaacagcta tataccccga tatttgcagt atcaaggata atcggatggt cagcacacag
    10321 gcttgaggag ctttccaaca atggtaagat aatccgtcct gcatatattg ctgtagctcc
    10381 tagaagagaa tatatcgata tcaataaaag ataataccgt tttttatgtt gcatataaca
    10441 ttaattaatg tataataccg atattatggt tattgtttta tttattatca tgttaatagg
    10501 tgtgctctta ggaaaaatga aggttataaa ttcggaatca agaagcacca tttcttttct
    10561 aataatcaat gtttcaatgc ctatgcttat agtttcttcc agcaaccttg agtttaacga
    10621 gcagacaaaa aatgaatcaa tgtggcttat aatcatgtca gtcgctgctt tcttcttttc
    10681 tattctactt gcccagctga tattcaaaaa agctgtacca agatgcgcta ctgttttttc
    10741 caatgctgga tacatgggac ttcctgtatt aaattccatt ttaggtccaa aaggattgtt
    10801 tcttggagca atatttcaaa tggtctttca tcttttcaca tggacttatg gaatctcctt
    10861 atttacaaaa tccaataaat ttaaagattc tcttaagaaa atgattaatc cttcattgat
    10921 agcagttgca atagcaattg tattgatgtt taccggatgg aagataccag acacaatcca
    10981 gactgtttta aagatgatgg gtgaactgac tactcctctt tctatgttac ttattggagc
    11041 aactcttgcc caagtcagtc tgaaggaagt tattaaacta aaacagattt atatagtaac
    11101 tgctttacgt ctgctgataa tacctgcttt cgtacttttg tttcttcttt tcccttcaat
    11161 accgaaaata tcagtatatg tagtatttgt aattaatgct atgccttcgg ctgcaataac
    11221 aggcattctg gcaatgaaat acaataacga tgagaaacta gcttcttcaa tagtagcctt
    11281 ctctaccctt atatcaataa ttactctatt cctgattact aatgtttttg atgtagcatc
    11341 agttttagga ttagtttaat gctttttcgc ttactttcat gaatattacc actaagtttc
    11401 ataatatagc ttcaagtaaa taaaaaatgt tatatattga acatcatatg tttgacacct
    11461 gcctttttgc agaatataat ttaattatta ttaagacaaa gaactaaata gaatatattt
    11521 ctttacagga ggaataacat gagaaaagga aaaacattta agtcactgac attacttttg
    11581 gctctgtgta tgattgtggc atcttttgtt ggatgttcgc ctaaaaaatc taatacacca
    11641 ctaacaatct gggttggtgt ggaatcagta gacttctata cagaaaagat ggcagaatat
    11701 gttgaaaaat acaaagaaca aacaggagag gatttccctt tctctgtttc agtacaggga
    11761 gtagatactg cttctgctgc agctaaagtt cttgatgacc ttgatgctgg agcagatata
    11821 tttacagtag ctcatgataa tcttggaaag ctcactgcgg gggcttcagc aatagctcca
    11881 gtcagaagcg aagcgttatt agcacagata aatgctgata atcctgaaac ttttcttaat
    11941 gtaattgaca tgacagtaca aggaacaaag tatactttcg gtatacctta cattgctcaa
    12001 tctctggttt taatgtataa cacaaaatat ctgtcagaaa atgatgttaa aacatgggaa
    12061 ggcatctggg cagctgcgaa aaataataat aaacaatctg tatctttgat gggagctgat
    12121 ggttttaata attcattcct tacactggca ataaatgaag aaacaggaga aatgcctgca
    12181 aaaatttatc aaaatggaga tatagaagat tgtgagttca cctcagatga aacagtcgca
    12241 ataatgcagt ggggtcagag attctttact gacaaaaacg gtggaaagat gcctacggat
    12301 tctggttggg aaattgaact tagagatgaa atatcattaa gccttatagg tggagcatgg
    12361 aaattcaatg ctgctaaggc tgctctaggt gaaaatcttg gtatcgctgc acttccttct
    12421 tttaccttaa cagctaacga tgttaaaggc accaatgtaa atgcaggaac tgtatacaga
    12481 agcggctcat ttactgactg caagatattt gtcatgaaga aaggaagcga taaagctgat
    12541 taccttgaag atatcctgct tttcttatca agcaaggaag ttcaggaaca gtcatttata
    12601 gaatgtgcta atcttccagc atataagaat gcgattacag aatttacttc aatgcaggaa
    12661 gacaacatcg aaacaattct agcagcaaaa cagctggaga tgttcaatta tggtattccc
    12721 cagcctttcg gatattcaac tactcataac ttctattatt attcaaaagg agctcctgac
    12781 cttgtaaggg aaatacttga gaacaaggaa agcctatttg gtactactga agctataagg
    12841 actcaattgc aaacaatcga aaatatctgg aaaacaggag aaagataaag catataaatc
    12901 gaacgggggc tatagtatgg ataataacaa aactggaatc aatcgattcc taacaaaaat
    12961 ttcaaagccg ttcgtaagat tctataatag atttatggat ggcagtcttg gcaccaagat
    13021 gtcacacttc gtgatgggtt cgggaaatgt atatcacggc cagataatta aaggccttat
    13081 atacttcata attcaagcag gttatgctct tttcatgata acctgtcctg aagtgaacaa
    13141 gacacctttg ggttacaagg cttttataaa ccttattaca cttggtactc aggagggtga
    13201 tattttcact cctgcagaca actctatgct aatgctttta tttggagttg tcactatcgg
    13261 gataacactg atgtacctgt tcgcatacaa ttccggaata aaaagcagtt ataaagccga
    13321 tcaggatata aaaaattatg gtaaagtgac atcattcaag caggatgcaa aagaaatgct
    13381 cggtaaccgt ttctacatta caatgctaac acctgctgct attggtgcag ttgttttcac
    13441 attattaccg acaatattta tgatacttat agcctttacc aattatgaca aagaaagaca
    13501 agccggttta aagctttttg actgggtggg atttgataac tttacaacat tcttcactaa
    13561 tcccacaggg gaaattgctg caagatttct gcctgttctc gcatggaccc ttatatgggc
    13621 attttttgca acattcttaa attatttcgg cggtatagta cttgctctta ttattaacaa
    13681 aaaaggctta aaaggaaaga agattttccg tgcggtattc gtactgacta tagccatacc
    13741 gcagtttgtt tctcttttag caatgagaaa ccttattggt gaatatggtc cattacaaag
    13801 ctttttagtt cagacaggca tattaagcga gcctatgtca tttttagcta atgctacaaa
    13861 tgtctggact gcgaggatta ccattatttt aataaatcta tgggtaggta tcccctactc
    13921 aatgctatta gcatcaggag ctatcatgaa tattcctgac agcttatatg aagcggcaag
    13981 gattgatggt gctaaaaaat tcactatact tcgcaagata actatgccct atattgtatt
    14041 tataacaaca ccatatctaa tatcatcttt tgtaggcaat attacaagct ttaatattat
    14101 atatcttctg acttctggca atccgaaggt aacaggagga tatattgcag gaggtacgga
    14161 tcttttagtt acatggctgt ataagcttac aattgatgaa cagcaatata accttggcgc
    14221 agtaataggt atacttacct ttctgatcac atcgatagtc accttagtga catatcgcag
    14281 atccaaatcc tataaagagg aggacacttt ccaatgagtg caaatacaaa aacaattaca
    14341 attgataaaa aagatgggaa taagcctgta aaggaaaaat tcttcaatca gaccagatct
    14401 gaaaaggtat ctaaaattgg cacatatata ttgcttatag ctttaagcat aatatggtta
    14461 taccctatac tttggatact gctcaatgct ttaagaacag aattcaatgt cgatggaact
    14521 atggttggta ctgttgtatc acactactgg ccaaaacagt tcggatttga taacttcaaa
    14581 atactattta ctaatacata ttttccaata tggtttagaa acactttatt tgttgcagta
    14641 ttctcatgtg tcctgtctac tatgcttaca ctttctgtag catatgttat gagcaaagtg
    14701 aaattcaaaa tgagaaagcc ttttatgaat attgctatag tcatgggatt atttcctgga
    14761 ttcatgtcaa tgatagcaat ttactatatt ttaaaagctc ttgggctcac tcagacattg
    14821 actgcactta tactttgtta ctctgccgga gcaggacttg ggttctatgt cggtaaggga
    14881 ttttttgata cagttccaaa tgcgcttatt gaggctgccc gaattgacgg agctggggaa
    14941 gcaaggatat ttggaagcgt tatattaccg ataagcaagc cgataatcgt atacactgcc
    15001 ctaatgagct ttactgctcc atggatggat tttatatttg caagagtaat acttggagaa
    15061 cagaacactg aacttcacac agtcgcagtc gggttgtact atatgatgta tggacaaatt
    15121 gttgacactt cacgattcac tgtatttgcc gcaggatgcg tctgtgttgc aatacctatt
    15181 gtagccttat ttatgtacct acagaaatac tatatagaag gaagcaccgc tggttcggta
    15241 aaaggatgat gaaaactata acaaaaaaaa tactaagcat acttgtgagc atcttaatga
    15301 tgctcacttt tgcctcatgc agtacagatg ctttaagagg tgaaaaagcc ataaaaatag
    15361 atgaattacc ggatattgat gttcctggcg tgtattacga aatctttgtt ggtggattct
    15421 ctgatagcga tgaagacggt acaggagaca tcaaaggaat tataaaccgt attgattacc
    15481 ttaatgatgg tgatccatta agtggaaaaa gccttggaat agaaggaatc tggcttatgc
    15541 ccatcatgca ggcaggctcc taccataaat atgatgtcgt agactattac agtatcgatt
    15601 taaattatgg gactatggag gatttcgaac agctcatatc cgtatgtgat gctaaaggta
    15661 tagacatcat aattgatctg gtcttaaatc acacatcaga gtataacaga tggtttctgg
    15721 acctgaaaaa cgcaattaaa gaaggagata aggataataa gtacatggac tggtacagca
    15781 tcacaactga ttcatccaag ggatggcata agcttgctga agatccagat ggagtccagt
    15841 attattacga aggcaacttc tccccttcta tgcctgaatt aaatatggat aatcccgaag
    15901 ttcttgacga aattgataat atcgtgaaat tctggctgga aaaaggcgta aagggtttca
    15961 gacttgatgc agttaagtat gtttatctga gtaatgacat taaaaatatt gagttttgga
    16021 aatggtttgc agacacatgc cgaaaatacg atgaggatgt tttccttgtc ggagaaaact
    16081 ggtcaaacac taccagtgta tatgaatatt atgaggcttt agactgcttt gattttgata
    16141 tggcaggttc aggtggtgat gtagccctta ctgtaaacgg aattttcaat ttaaatgatt
    16201 atgttagaaa catatgccgt tacaaggaaa atgtggaaat ttccaatcca gatgcaatat
    16261 taaaaccatt tatctctaac catgatatgg atagaagtgc aggttatctt tccgttaagg
    16321 aaaacagaat gcacatggct gcaaatctat atatgctttc atctggtaac cctttcatgt
    16381 attatgggga agaaatcgga atgtatggaa gcagagctac ctcctccact gacgctaaca
    16441 ggcgtttagc aatgctttgg ggagataaag atacagtaca agatcctcca ggaacaacat
    16501 tcccagctga aagacagatt aacggtacag taaaacagca gatcaaacag catgacagcc
    16561 tttacacata ttacaaaaaa ctcattgcct taagaaaagc aaatcctgaa attgcagtcg
    16621 gtgattatat tccgcttaag tttgaaggat acaatctttt cggcgggttc cttgttcaat
    16681 atgaaaacgt tactaaaggt gttttccaca acctgtcgaa tgaacctata actattgatc
    16741 tgtctacata taccgatatt aagtttgatt caattagcgg atatatagga caaggcaaag
    16801 ctcgtctgaa tggtaacatt cttacacttg atgggttgac atctgtaatc ctaaaataaa
    16861 ctgaaataat tgtgaaaaag caaaagttca catttttttc acaattcaat tgaaatctct
    16921 tgactctgtg taaggccgtg ataaaatata gttgttcatt tggaaaaata tatcaattga
    16981 aaattgagga ggaattatga aaaagttcaa agttatcgca attgtattaa cattggttct
    17041 agctcttgga tcattagccg catgtacacc tgacactatc cttgaaaaca ccgaaaaaga
    17101 ctattatgtt accggacagt tcgcaggatg gggtgatgct gttggaaatg atacttataa
    17161 gatgacacct gtttcactta aggatgcacg tgttgctgca cttaaagctg atttaaaagg
    17221 cgcgaaatat ttatacatac ttgaaaatgt aaccattact gccgaaggtg caggatggtc
    17281 agcacagtat gtagaaaatg gtgctgtaaa agaagctgat ggaaaccaga caatgaaatg
    17341 gttacaagtt tcaaaaggcc aggaagctcc agactggtgg gcacagagtc ctgaaagcgg
    17401 agaaattgtt tctctgactc ctgatcttct ttggattcct ggattcacag aaactcctga
    17461 agtaggtcca gactggaacg gaaaccctgt agtactcaaa gctggcacat acactgttgt
    17521 atttgctata gttgagaaag aagaaggatt ggttaaggta gcaggcctta tagccgaata
    17581 attaaccaat aacaaacaaa gaagcggctt aaaagccgct tttttttgcc ttgatttgtt
    17641 actccttgta ttcacatata tgctgttaat tgttgtttca tttagaaacc tcatagggca
    17701 tgaaattatc ttaaatcata atactaaatt ctatttttat tgtatttcat atatagaatg
    17761 taaaaaagta atagtccaat aacaaccaga gctgctgctt gaactacaca ccattgtcct
    17821 aatgacataa ggttattccc atatagatat acgttaaata gtatgttaga aaaactatct
    17881 gctcccatga aagctaaaaa tgcaggaata actataactg gtagcgtaat tgcaatcgtc
    17941 attattcttt cacctgtaat atgagataca aaattaataa tgcttattga gataacaata
    18001 ccgatgaatt taaggataaa taatagtatg aaatattctg atatgcttac attaagaggg
    18061 aaattttcca taccatttat acatgctata ggagaatcaa aacatgcaaa tccgctttct
    18121 cttgcaattt ttataatcag tggaatatat gctgctgaca tcacaagaaa agctattata
    18181 caggagataa tgctcttatt aataaaagtc cgttttctac catgcacagt gctggttatg
    18241 atactatatg tattattttt tttctccatt gcgaaaatat caaccgtaca aagaataaca
    18301 agtaaaagtg aatacaatgc tgcctttctg cttaaataat catcattgaa aaattcgtta
    18361 tatccttttt cataaacaaa agatattctg tttttaatat tgatatcctc tgtagaatct
    18421 ttaagcaggt ttaatgatga atctttagaa atcaaattaa gtattactga aatcttatcc
    18481 ctatttaaac tgcttgcatc ctgattattc tctgtttgca cttctttcag cattttttct
    18541 agagtaagtg ttttttcatt tgaaataggt ccttcaagtt ttttcatata atacatatat
    18601 atgtaatctt ttgttgcaag tctttgttcc ggaggatatg ctttgacagt tagcaattga
    18661 aacagaagga aaatgattaa gattggaagt acagggttga ttatgatgca tttatatgct
    18721 tcgaaacaaa agattccggt atttatcttc tttgccttac ttgtcttgat tttccttata
    18781 tttcttttta aatacgaccc ttgtatatct ttttgtcctt tgaaaatatt tatagttacc
    18841 agtgaaagaa ttacagaact tgttatgacg gttatgaaaa aaatcgggac aaaagataaa
    18901 ggataaccag ccatatttat attcagataa ttacttataa tttttcctgc atccagaaga
    18961 aagtatagat tcaaatactt aaatatacta aaaaatgata cagatgatat tgagttatgc
    19021 ataacaattg atatggtgaa aattgaagca acaatgataa ctcccactat tgaatcctta
    19081 attgatacaa agataaacat cataagtgtc aatacgaata ttacagaaag catcagcata
    19141 gggatataga caatgagata ttgaagaacg ctcagttcaa atatacatga ctgaaacccg
    19201 aatacacttt gtaacggcat attgatatcc ccaaatccat ataagacgta acctgtcaag
    19261 taagaagaaa ctgaaagaag aaatgttgta actaaaccaa gtatcaaagc actgattatt
    19321 ttcgagagca tgagatgttt tctgccttta cgcattgttc taagtaatgt taaagtattg
    19381 ttgtccttat catggatgta gacaagaaaa catataaata aacaggcgac taaaagatat
    19441 ataggtgtta taccgttttt agtcgctaaa gttacgcctt ttgagctgtt gaatcttaag
    19501 tttgaaatat tcaggtttga aaatgcttct gctgtcttct ttgcattatg cttcgcaaaa
    19561 tgcgaatcag caaatatagg tgatgtctgc atacgtattg caattctgtt tatttcttcc
    19621 ctgtattcat catatgagta tacaacatca acttcctcga gcttctgacg aagaactatc
    19681 ccaattgcat atggattttt aatataacca tcatatactc cattgttgta atcagcgatt
    19741 attttttcga tattatttcc aggatatcga agaaccagtt tctcaggagt caaaggtatt
    19801 tcgcttgtta gagataatgc atattccata gcaataatat gctcatttaa aaaatcctta
    19861 gcctgatttt cgtccaaatg cataagcttt tcatcaatat ctctgacgat gctcaaatcc
    19921 aaacttgagc ctgatgaaac actctcgttt atataaagag taattacatt aattgtaact
    19981 gcaattataa ggaatattac tgttgtctta ctgacaaatg ttttttttaa ctcacataac
    20041 agaagattca ctgcataacc tctgtttccc tctcctcttc aaaaaccgaa agataaacat
    20101 cttcaatggt agctcttgtt tgcctatgac tgtatttgct taaatcagat tcagtctgat
    20161 gttcatgaag caagatacgc actatcagat tatctacact tttttggata ttacagacat
    20221 tgaaagataa gataatatcc agaagttcta cttcacttgc ttcaatttca tacactttat
    20281 cttctaattt tttacatagt tcataaggat aatcataatt aatcaaaaca ccttttttta
    20341 taagcataac ctttttagca atatattcga tatctgagac tacatgagta gcaataatga
    20401 caattctgtt aagagcataa ccggatataa gatttctcat tctgatacgt tctttcggat
    20461 ctaatccggc tgtcggctcg tctaggatca aaatcttagg atctcccata agagcctgag
    20521 caagcataat cctttgtttc attcctccag aaaaagttcc tatccgtcta tgtgcaacat
    20581 ccaaaaggtt aacggatttt aatattttag gtacttcatc ttctattctc ttcttatcca
    20641 ttgcttttag agaaccgata taggacaaaa attgagctgc agtgaatgag ggatatatac
    20701 catgatgctg gggcatatat cctaacatag acctataaga atcccctata gttttaatgc
    20761 tctttccatt aatatatacc gaccctgagt cacttttaag actgtccgta atgattttga
    20821 tcaatgtcga tttgcctgcg ccattggggc ctagcaagcc atatactccg tcatcaagta
    20881 cagctgtaaa accattaaga gcttttatta gaccatatga cttatttata ttctcaagca
    20941 ttagttccat aatttttagc ctttatcagt aaataacatt tatatctaca aaatcatatg
    21001 atttatttat cagtgattct atcttcatat atctgtaact ggcatgttta ctgccctcgt
    21061 caaatttata taataaaata aattcttcat tcagatttat caaatgagta actaatcctg
    21121 tctgtactcc gctataatgc atatcacatt catttatcgt tttctcatcc gaatcatata
    21181 ttacaaactt ccattctgtc tcttcgggta ttttactttc attactgtga ataaagaaat
    21241 acttaccgga aatgcctatc aatgtcccat cctgaatctc aataccagat acgaaaattt
    21301 catcttctaa agtcataaga tcagtccttc ttatgtcagg tgacgattgc attggattac
    21361 tttttatata gtacaaatac cctttgtcca ttacgcaata tgctttttcc tgttcatttt
    21421 tcaaaggtag tgaaagcgct tctaaaacag tgaaatcaga aagacttaca cgtcgtattt
    21481 cataaacatt ttcatccata taataataat gaaggacatt attatagaca taataacttt
    21541 gtgagatttg atgtttctca tatgtttcta tttgaacaag ctctagtgta ttgatatcaa
    21601 taattccagt aaaatactta atttgtatag tactgaccga aagctcatct tcattatcct
    21661 ttttaactaa ttcctttcct gtaaatacaa aaagattctt ataaactatt gccttatcaa
    21721 taattccacc gcaatttacc tgtcctgctt ttattctgtt ttcaccatct aaatctgatt
    21781 tatatatatc cattttattt atttcggatg gaagcacaaa catatataaa taattattaa
    21841 caattactcc agcttcaaac gtcgtatttt ttggcggtac tgcagcacaa gacggattag
    21901 ggtttgtttg actgtcatat ttctgatggg agcactcagg acggctgcat acgacacttc
    21961 tttcaccagt tttataatcg taataagcaa gcaacccatc ctcgtatatt atcattccat
    22021 tttcgccgta tgcagatcct tcatacgtaa cagccgtatc gttattattg ctttgttttt
    22081 tattgcatcc acataaaaaa gttaatataa ccgcaattaa tataatcagt agataaattc
    22141 tcttatttgt attcatgatt caatccttat ctcagggctt actttctata tattgcccaa
    22201 gttgcttatt tatatcctgt aaaaacctgc tataccctaa tgcatccaat tgcgttatta
    22261 cattttcaag ttcagacaga aaattatcag aatgtgtagc ccataaacta tctgcttctt
    22321 tacataatct gcttatttct tcaaattcat attccatttt cgtaatatct ggttcaaaac
    22381 cataattagg aggaatatac gcatcactca ttgcatcttt aaaatcagat atcctttttt
    22441 ctccaactct atttggatat agcatatgag acgcagtaag cacggaagtg ctataatttc
    22501 cgttttgttt tacaagacca ttttcacatt tatagttttt accataatat attaggtttg
    22561 aaagatgtgg gtctgtaaac aaagtagaca gaaaatcaaa tgcactttca ccatttttcg
    22621 tccatgaagc tatacctata agtttgtttc tactgtcgtg caatatatat ctgcttattt
    22681 caactggtat atattgaata tcatctgaag aatcgttaga atagtcttga taaccaggta
    22741 caatatatga aatatatgga gcaaatgcta tgcttgcaga ggcggaaccg tcagtattat
    22801 atacaatact tgtgttaaaa taccctcttg cagcccattt tgcaagtatt ttgtttattt
    22861 gtaaataatc agcatcctta aatctgttta ttactgccgg attgttatct gcatcatatt
    22921 tatatgaaat agtcgaatta cttttatcag taattatccc ggctaattca ttatatgcag
    22981 agtaatctaa gaatttacga ataagcatca aatcactgct tgttatctct gttttatagt
    23041 cgtcaaatat ctcgtcaaag aaaacatcaa tttcatcaat cgtatcaagc tcggaaatat
    23101 tgacttcata tttatttatt atgtcatttc tcagataaaa aatgaaactg gatgctatct
    23161 cattgttata tacaccatat ataccgttat atttatcaaa taaactccag tattcgtccg
    23221 gaaaaagccc gtataatttt ttatcttcat catctaacag aaaatctgta atatccagta
    23281 aaagatcatt attaatctca tctgctccag attcttcaat tgcagtataa tttacctgta
    23341 ctgtcacatc attttcttta atagaaaaag ttccttttat atttgcaata gagctgatga
    23401 tatcagcctg tattcccgaa tttttcatat ctttcatgaa ttcgaaataa taagcatata
    23461 tttctgtgaa tttaaaatat tgttttgcat tattatcatc accaagagct ttataatcct
    23521 ctgctatctt gtcaactata gcatcctgtc gtataaatct gataacataa tctttcccaa
    23581 gtgaaattaa gtaatcatta tatgcaacta ttagttcatc actaacaata ctggtataga
    23641 attcactgaa tggttttggg tttgaattat ctaggttatt tatgtattgc aatatgccaa
    23701 aataatcaaa atataaccat acaagcattt tcttttctgg ccacatagcc tgataatcca
    23761 aattgccctc tgtatcatat tttatactcc tatcgacgga agtggagtgt gttggcaata
    23821 aggaagcata ggaatctgtc accaaagaag gtgatgcgtc caatgaaggc gtagtactgg
    23881 tgtttacctc attattattg atacatgaac tcaatgaact caataataat ataattaata
    23941 taatacatat actctttttc attttaaatt aatttaattc gatgcagata gataattatt
    24001 atcacaactt ccatttatat agccataatg ataactatct gctcttgtaa acgttctggc
    24061 acctgagggt agaattaaaa ctgcagcaac accatctaca taatcttgat actcagaata
    24121 atttgaactg tattcattac cataataatc tacaaaccaa acagttccac taactcccac
    24181 gctcaagttt atcgtaggag gtataggatc aagatttttc tctgcagtta caactaaatt
    24241 accaacactt aatgatccac tccaatatcc tgaacctaaa ggttcactaa atgaactgct
    24301 gccagcaaaa actgataatg ctgtaaacaa caaaataaaa aataataaaa ttgaaaacaa
    24361 tttcttattt ttcataatat agtcctcctg tttatgtgat acaaaaaaat tatagtaccc
    24421 gtctttgaca gttagcacca taatctttag gagcaaatac cgtatacaat caaaaacaat
    24481 cccttaaata agggatattt ttttaaagaa aaagcttgac aa
//
LOCUS       DAOSXM010000027        24139 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2111
            NODE_1335_length_24139_cov_11.886065, whole genome shotgun
            sequence.
ACCESSION   DAOSXM010000027 DAOSXM010000000
VERSION     DAOSXM010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864178
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 24139)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24139)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:24:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,261
            CDSs (total)                      :: 2,229
            Genes (coding)                    :: 2,218
            CDSs (with protein)               :: 2,218
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 3 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24139
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1335_length_24139_cov_11.886065"
                     /isolate="AMR_MDS_2111"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1522)
                     /gene="guaA"
                     /locus_tag="PLQ68_05345"
     CDS             complement(<1..1522)
                     /gene="guaA"
                     /locus_tag="PLQ68_05345"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004694093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HXK72108.1"
                     /translation="MKDRQTVIVLDFGGQYKDLIARRVRELGVYSIILSGDTTTKEIF
                     SYKPIGIITTGGPKSVYKPDSPLCDNEFLESGIPILGICYGMQMMAYVLKGEVQSAAV
                     SEYGQTCGTVVSESVLFDKVGNDLKVLMSHTDRITKLPDGFIATASTKDCPIAAMENK
                     GKNLYGVQFHPEVTHTLKGKTIIRNFLYNICNAKGDYNMKNYIETAIEEIRKQVGNEQ
                     VLLGLSGGVDSAVCAALISKAVPNQLVCIFVDHGFMRKNEAEEVIEAFADKSLKLIAV
                     DASERFLKKLEGVTDPERKRKIIGAEFVEVFNDEALKLGRIKFLAQGTIYPDVIESGN
                     GKAATIKSHHNVGGLPEKMGFSGLVEPLRGLFKDEVRKVGRELGLPEAITERQPFPGP
                     GLAIRIIGELTKEKLDMLREADYIYREELGKLKKKPSQYFAVLTNTRTVGVMGDERTY
                     GYVLALRAVVTGDFMTADYAHIPQSVLSRISRRITNEVHGITRIVYDITGKPPATIE"
     gene            complement(1512..2096)
                     /locus_tag="PLQ68_05350"
     CDS             complement(1512..2096)
                     /locus_tag="PLQ68_05350"
                     /inference="COORDINATES: protein motif:HMM:NF015799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ANTAR domain-containing protein"
                     /protein_id="HXK72109.1"
                     /translation="MTRHETSSILIVAASEKHMEFFNCTLCDESFYAKTYVKTANEAR
                     NLFMNRSFDAVIINTPLTDEFGTELAKHISQSSDCGVVVLVRNESYEEISYSLESYGI
                     LVVAKPVIRTVFLQSLRLALATKERLMILERTNEKLKAKLEQMKLVNHAKWVLIQYLK
                     MDEYQAHKYIERQAMDRRVTLKEVAEGIIKTYEG"
     gene            complement(2110..3345)
                     /locus_tag="PLQ68_05355"
     CDS             complement(2110..3345)
                     /locus_tag="PLQ68_05355"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016439171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HXK72110.1"
                     /translation="MYYSKDEAIQFIEQNDVKFIRLAFCDIYGRQKNVSIMPGELKKA
                     FEGGISFDASAIEGFKEESKSDLFLIPDAKTMSLLPWRPTSGRVVRFFCDVYDEKRNP
                     YIADTRYILKQAVSFAAQQGYSFLFGPECEFYLFKTDEDGNSTNVPIDNGTYMDIAPD
                     DRGENVRREICLTLEQMGIKPEKSHHEEGPGQNEIDFAYSDALSAADNMTAFKAVVSA
                     ISARNGLYADFSPKPLKDKAGSGLHINMSVKSVTKHDVTTKERDSMVAGILKRIREIT
                     LFLNNSDNSYQRLSKFKAPGYVTWAFSDRSQLIRIPASSEAASRIELRSPDPLTNPYI
                     TFALLIYSSLEGILQAMTPPDAMDMNLYKTNCDMVKGIERLPDNLNDAKKLASSSEFV
                     DKYIPKQIIEGYLERRNKD"
     gene            complement(3385..4053)
                     /locus_tag="PLQ68_05360"
     CDS             complement(3385..4053)
                     /locus_tag="PLQ68_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004611498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase"
                     /protein_id="HXK72111.1"
                     /translation="MKSVIKMDNTNCLGVRYIACGDKENEYIIEGTPGNALAAYLDGG
                     NVTVYGNAQDATGDTMNKGQITIHGSTGDAVGYAMRGGQIFVKNDAGYRAGIHMKQYE
                     NKLPVLVIGGTVGSFLGEYQAGGIIIVLGIGHEEKSVAGNFCATGMHGGLIFLRTKNL
                     PKSMPSQVTVTKANEEDMEVVKEYIEKYCDMFGEDIKNIMESPFLVIRPNTNNPYKQL
                     YCYS"
     gene            complement(4055..5290)
                     /locus_tag="PLQ68_05365"
     CDS             complement(4055..5290)
                     /locus_tag="PLQ68_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007050598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HXK72112.1"
                     /translation="MNYVIIGNSTAAIAAIEGIRSIDKQGRITLISIENHHTYSRPLI
                     SYLLQGKTDFDKMKYRPDSFYEDNSVTVVLGKKAVKVIPDMKAVILEDSSSYEYDRLL
                     IATGSLPFVPDISGLDKVKSMHTFMTLDDALELEKSINDKSNVLVLGAGLIGLKCCEA
                     IKDKVASVTVIDLAKQVLPSILDKTSSDIVAQHIEKQGVKLKLSCSIKQVYENSALLT
                     DDSVVRFDALVMAVGVRPNISLLRAAGVDTNRGVLINQCCATSIEDIYCAGDCAEGYD
                     MTADCKKILALLPNAYMQGFCAGVNMAGEEKIYLSGMPMNALSLMGLHILTAGIYEGE
                     EYVFQDKKGYKRLFYKDDMLKGFILVDDIECAGIYTAMIRNKTLLSSMDFDLIKKKPA
                     LCALAQERRTSLLNRKETW"
     gene            complement(5292..5720)
                     /locus_tag="PLQ68_05370"
     CDS             complement(5292..5720)
                     /locus_tag="PLQ68_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HXK72113.1"
                     /translation="MKMIKVNEKWCLGCHLCEYYCAFANSGVEDMVKALKDMMIQPKL
                     RIEQKDKISFAVSCRHCEDPICVKSCITGALSIIDNVVSIDKKRCIGCYTCVLVCPYG
                     AVMPSQEGAVRKCELCVGNSVSSPACVSACPNKAIELVEV"
     gene            complement(5722..7227)
                     /locus_tag="PLQ68_05375"
     CDS             complement(5722..7227)
                     /locus_tag="PLQ68_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HXK72114.1"
                     /translation="MPINYIIPQFEIVRDQSRCIKCRVCERQCANEVHHYDEDYKIMV
                     ADESKCVNCHRCVALCPTSALKIVKNQDSFKENGNWTAEIITDVYKQASSGGVLLSSM
                     GNPMKFPVYWDKLLINASQVTNPSIDPLREPMETKTFLGQKKHEIKRDNEGRIISNLG
                     PQLELSTPIMFSAMSYGSLSYNAHESLARAAKSLSILYNTGEGGLHEDFYQYGSNTIV
                     QVASGRFGVHKDYLNAGAAVEIKMGQGAKPGIGGHLPGTKITEDVSKTRMIPVGTDAI
                     SPAPHHDIYSIEDLNQLVMSLKHATDFKKPVIVKIAAVHNVSAIASGIARSGADIIAI
                     DGFRGGTGAAPVRIRDNVGIPIELALASVDTRLRNEGIRDNVSLVVGGSIRSSADVVK
                     AIALGADAVYIATAALLALGCHLCRTCYSGKCNWGIATQREDLVKRLNPEIGYERLVN
                     LINAWTHEIKEMMGGMGINSIEALRGNRLMLRGIGLNEKELEILGVRHAGE"
     gene            complement(7227..8324)
                     /locus_tag="PLQ68_05380"
     CDS             complement(7227..8324)
                     /locus_tag="PLQ68_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase family protein"
                     /protein_id="HXK72115.1"
                     /translation="MQREGTDNYRIPSGCAICGIFNRDGKRISGSDIIQAIAIMHERS
                     NGLGGGFAGYGIYPEYKEYYAFHIFYDDDRAKVTCEEYLNKHFDVVNLSRIPTKKHPK
                     IVNEPLIFRYFVLPHPADLFSSQLDEQEYTSLCVTRINTDIEGAYVFSSGKNMGVFKA
                     VGYPEDVGSFYKLDEYNAYCWTAHGRYPTNTPGWWGGAHPFSLLDCSIVHNGEISSYD
                     ANRRYIEMFGYKCNLLTDTEVIAYILDFLVRKLGLTYSEAASVIAAPFWSTIDRKDKE
                     EKERLTYLRNTFSSLLITGPFSIIAGFSGGIMALNDRLKLRSMVVGEKGPAVYISSEE
                     SAIRKICKDLDSVYSPRGGEPVIYTLRGGIS"
     gene            8637..10739
                     /locus_tag="PLQ68_05385"
     CDS             8637..10739
                     /locus_tag="PLQ68_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004609509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III"
                     /protein_id="HXK72116.1"
                     /translation="MEYTTHDVPKLFGSAVFNDAVMRSRLPKDIYHSLKKTIEEGTDL
                     DINSANSVASVMRDWAIEKGATHFTHWFQPLNGVTAEKHESFLSAQSNGTVIMEFNGN
                     ELIKGEPDASSFPSGGLRATFEARGYTAWDPTSYAFIKGNCLCIPTVFCSYCGSVLDK
                     KTPLLRSMEQLNEQALRILKLFGHSDVTHVASTVGPEQEYFLIDKEMFEKRKDLKFCG
                     RTLFGAKPPKGQEMEDHYFGAIRPRVEAFMQDLNNELWKLGIFAKTEHNEVAPSQHEL
                     APIFTTTNTATDHNQITMEILKRTAEKHNMACLLHEKPFAGINGSGKHNNWSMSTNTG
                     KNLLDGGKTPSSNKQFLLILTAIIKAVDEHQDLLRISVASAGNDHRLGANEAPPAILS
                     IFLGEELTGILESLANDLIYNGNGHVSMKTGVHAIPGFRKDTTDRNRTSPFAFTGNKF
                     EFRMVGSNMSIADANIVLNTIIADSLSSLADELEKADDIDKAVDMLIKKTYVDHRRIV
                     FNGNNYAPEWVEEAEKRGLLNLKTTADSLSMFISKKNIELFEKYGILSEIELRSRYEI
                     LLENYCKTINIEALTMAQIAKRDIYPSVCGYLKTLLDLLNSKKQAGIESSCDSLLSHI
                     KKLSSLSDSLYGEISELLDAVMNQNEISSSHELSFYCKDTIIPIMNRLRAVADELETQ
                     TSSDLWPFPTYSQILYSV"
     assembly_gap    10820..10919
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(10996..13428)
                     /locus_tag="PLQ68_05390"
     CDS             complement(10996..13428)
                     /locus_tag="PLQ68_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016426614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HXK72117.1"
                     /translation="MTNYAIKAEYVEKAEKIVTKMSLEQKASLCSGKDFWHTKEVNEH
                     SVPSIMMTDGPHGLRKQKDESDHLGINESYPATCFPPACTTACSFDVELIGEMGAAVA
                     RECLDQGVSIVLGPAVNIKRSPLCGRNFEYFSEDPYLSGKMATAYINGVQSKNVGTSI
                     KHYLANSQEKLRLVSDSIVDERALREIYLPAFEDAVKNAQPWTVMCSYNKINGTYASE
                     NKRFLNDILRDEWGFKGAVVSDWGAVNNRVEGVSAGLDLEMPYSGTENEKAIISAVKN
                     GLLDIKALNKSAARMVAIALASNDVEKENCDMDANNELARRIARESAVLLKNNDILPL
                     KKDTKVAIIGEFAKKPRYQGAGSSHVSSYKVTSVVEEFANKGINFEYARGYDANSDVP
                     DMQLISEAAQTAKKADIAIVFVGLPDAYESEGYDRTHMELPPSHNMLIESVCNANRLT
                     IAIIYSGAAIVMPWMKLVKGAIMMYLTGQNNGGAVYDLVFGDYSPCGKLAESFVKSLE
                     DNPSYRWFAKKKNAEYRESIFVGYRYYDKANKEVAFPFGYGLSYSNFTYSNIKLDKNK
                     MKDDETLNVSFKIKNTGHMEAKEITQLYIKSSSEIVFRPIRELKGFSKVVLKPTQEKT
                     VTFTLDKRAFAYFNTYSADWHVESGIYTVEIASSSRNIHLSAQVEIEGNRDINSLDMR
                     SVAPAYYALGKETLDIPDNQFRAVLDFEIPNEPSNRPYTINSTIKDIQTSFIGRLLVK
                     LLEGYAKKIMKDMTKDGDSMKKMIEGMIYDMPLRSLSTMTGGMFTHEKVESIVTMLNG
                     NVIKGLIGVLKK"
     gene            complement(13425..14798)
                     /locus_tag="PLQ68_05395"
     CDS             complement(13425..14798)
                     /locus_tag="PLQ68_05395"
                     /inference="COORDINATES: protein motif:HMM:TIGR00792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXK72118.1"
                     /translation="MGSRSDYSSSLSERFSYGLYFLGQNFFYVLVAMFIQVYFTDMGI
                     TASAVAIVLLVTKIWDAVNDPIFGIIVDRTRFKKGRFMPWIRISLVFIAATTMFIFFM
                     PISASPVAKIIWAVVSYILWDIAYTVCDVPIFALSTSMTDNINERTAILSIGRFMGTI
                     GSMAVVVLLPTIRPILGWTATAAIITIAGFIFMIPLCFIGKERVKVRKQEKITYKQMI
                     RYVKNNKYLLIFYLSYILIAFANSSQVVAIYFARDCLKDESMATIVSIFFVVPALIIA
                     LLIPSITRKIDKYHIYFWSIAGMGVFSIISYFVGYENLTAFYIFIALKGICIGGFTIL
                     LYMFTPDCVEYGTYMTNERAEGITFSIQTFTIKLMSAVTTAVAMLVLGWFGFKEGLNV
                     VQSEATLYGEWVMFSLFPVIGVILGLPVLRMYKLRSKDVQIMAEYNQGNITKEQADQL
                     LEGRYTK"
     gene            complement(14968..15255)
                     /locus_tag="PLQ68_05400"
     CDS             complement(14968..15255)
                     /locus_tag="PLQ68_05400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72119.1"
                     /translation="MDEKGSKSSKVMKLAIVLFAIGFEAISFELFMRLVYMNEKYMAR
                     WQMWVWFVVGVVFLIPSVYLFYKANKIKEAERNAYYEEFFKEEEKNISIEG"
     gene            complement(15403..16161)
                     /gene="hisF"
                     /locus_tag="PLQ68_05405"
     CDS             complement(15403..16161)
                     /gene="hisF"
                     /locus_tag="PLQ68_05405"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012676316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HXK72120.1"
                     /translation="MYTKRIIPCLDINKGRVVKGINFLDLIDAGDPVETAKAYNAKGA
                     DELVFLDITASYEKRDITLSMVEKVASEVFIPFTVGGGIRTTDDFNVLLRAGADKVSV
                     NTAGILNPSLISKASELFGSQCVVAAIDAKREEDGSYMVYINGGRDKTSKNAVEWAKE
                     CEKLGAGEILLTSMDADGTKKGFELSLTAMVSEAVSIPVIASGGAGSINDFYDVLTIG
                     KADAALAASLFHFHELDIIELKQELKNKGIPVRI"
     gene            complement(16183..16887)
                     /gene="hisA"
                     /locus_tag="PLQ68_05410"
     CDS             complement(16183..16887)
                     /gene="hisA"
                     /locus_tag="PLQ68_05410"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003491222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HXK72121.1"
                     /translation="MIIYPAIDILNGKCVRLFKGDYSKVTEYSKNPLEVAIAFMNAGA
                     THLHIVDLDGAKTGLSVNTEIIKSICSNTNLLVQTGGGIRTMERIKELISAGADRVVL
                     GTAAIKNPHLVRLAVKEYKEKIVVGIDALNDKVSVDGWTSSSDQNAISLAMIMKDMGV
                     SSIVYTDISKDGTLEGPNIIALKEMILKTGLDVVASGGIKDIDDIEEVKKTGAYAVIT
                     GKAIYEGKISLESLFK"
     gene            complement(16884..17504)
                     /gene="hisH"
                     /locus_tag="PLQ68_05415"
     CDS             complement(16884..17504)
                     /gene="hisH"
                     /locus_tag="PLQ68_05415"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013297100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HXK72122.1"
                     /translation="MIAVIDYGMGNIGSIIKAFSFIGQKVFIVNDPDLLNKYDKIVLP
                     GVGAFEKAMENLNSKGMADAVTEQIKKGKTFLGICLGYQLLFETSEEEFSEDKHLVTG
                     MGLLNGNVKKFREDISLKIPQMGWNSIKTNENMRILKDFDEKYFYFVHSYYASIENSS
                     EQDYALTQYGITFPSAVEKDNILACQFHPEKSGDEGINLLRKWVEQ"
     gene            complement(17501..18346)
                     /locus_tag="PLQ68_05420"
     CDS             complement(17501..18346)
                     /locus_tag="PLQ68_05420"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HXK72123.1"
                     /translation="MNIKPFKKGKVRDLYDLDDKMLMVASDRISAFDCVFDEPIPTKG
                     IILNSISAFFFEYTKDIIENHLISADISDFPAPFNEDEYFKGRSMLVKKIKMLPVECV
                     VRGYLEGSGLKEYNKTGSVCNIPLKEGLKQADKLDEPIYTPSIKAEEGHDVNTSYEET
                     VKMIGADLALKLKKKSLELYKKVSDYALTKGIIIADTKFEFGVLDGRLVLADEIFTPD
                     SSRFWDVDDYMPGKAQKSFDKQYLREYLETTGWDKNPPAPKLTDEVITQTLNKYMQAY
                     ERLVK"
     gene            complement(18346..18930)
                     /gene="hisB"
                     /locus_tag="PLQ68_05425"
     CDS             complement(18346..18930)
                     /gene="hisB"
                     /locus_tag="PLQ68_05425"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009610409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HXK72124.1"
                     /translation="MRKAVTKRKTKETDIFLELNIDGKGQYEIETEIGFFNHMLELFS
                     KHSLMDMKLVAKGDVFVDCHHTVEDTAIVIGETIKKALGDKSRITRYADILLPMDETL
                     TSVAVDLGGRPYTVFNCPVKQGRIGEMDFQTIEEFIVALSNNLGANIHVNVLYGSNLH
                     HIAESIFKALARSLRQAVKIDENETGIPSTKGEI"
     gene            complement(18927..19982)
                     /gene="hisC"
                     /locus_tag="PLQ68_05430"
     CDS             complement(18927..19982)
                     /gene="hisC"
                     /locus_tag="PLQ68_05430"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014489269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HXK72125.1"
                     /translation="MSNIYSDVLNRLSPYTPGEQLNDKRYIKLNTNENPYPPSPKVIE
                     AIKKGANSGLRLYPDPESIDLIETLADFHGVGKENIFVSNGSDELLAFAFNAFYANKD
                     VAFPEITYSFYPVYCSLYNCKARTVPMKDGYMIDKKALSEMDCPIIVANPNAPTGELL
                     ESEYILSLLKRNRDNLVLVDEAYIDFAKQQSMVPYVLDNENLLVVKTFSKSYCLAGMR
                     IGYAIGSKLLIDALKTIKNCFNSYPLDRLAQISAKEAVTDQTYYNNINQMVIDTRERT
                     KTALEQMGCVVLPSKSNFIFVSFPGFDSYTIYTKFRDKGILIRQFSYISDYVRISIGT
                     DSDMDILINLAKEIMKK"
     gene            complement(19975..21261)
                     /gene="hisD"
                     /locus_tag="PLQ68_05435"
     CDS             complement(19975..21261)
                     /gene="hisD"
                     /locus_tag="PLQ68_05435"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HXK72126.1"
                     /translation="MIPLYRNDKLGFINKVRKDGFLDNKEVYDKVFQIMEDIRTKGDE
                     ALLYYTKKFDNITLNSDQLIVTKEEIEQSYKIIDKELLKIIQQAIINIASYSMKQKED
                     SFLWNKTEGISIGQKVTPLKSAGIYIPGGTAPLISSVLMNAVPAYVAGVKEIVMCTPP
                     VIDAARICAANEAHVTKIYKAGGAQAIFSMAFGTQTIPKVDKITGPGNIYVAYAKKMA
                     YGYCGIDMVAGPSEIMIIADETADPRFIAADMLSQAEHDKLSCASLLTVSKELADEVQ
                     NQIVIQAEKLKRKDIIKKSIEDNSAIYVGKDIMECIEVLNECAPEHAQIMTKDADFYS
                     DFITQAGAVFVGPYSPEPLGDYFAGPNHTLPTAGTARFFSVLGVYDFMKKSSIIQYDK
                     KALNNAYKGISLFAKCEDLDAHANAVDIRFEGDENE"
     gene            complement(21245..21895)
                     /gene="hisG"
                     /locus_tag="PLQ68_05440"
     CDS             complement(21245..21895)
                     /gene="hisG"
                     /locus_tag="PLQ68_05440"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HXK72127.1"
                     /translation="MEYLNIALPKGRLAIQTIEFFKKSGIPCKNIDEDSRKLIHIDEE
                     NKLKIFLVKAADVPVYVEYGAADMGVVGRDTLMEESRNVYDVLNLGFGACRMCVAGEE
                     KYFGKTDEIINKRVATKYPKIASEFYRNVKNENVEIIKLNGSVELAPLCNLSELIVDI
                     VESGKTLQANGLMVLEKICDISAHLIVNKVSMNMKMSKISYIIQALKKELRNDTAL"
     gene            complement(21883..23121)
                     /gene="hisZ"
                     /locus_tag="PLQ68_05445"
     CDS             complement(21883..23121)
                     /gene="hisZ"
                     /locus_tag="PLQ68_05445"
                     /inference="COORDINATES: protein motif:HMM:TIGR00443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HXK72128.1"
                     /translation="MVFHNPEGMNDIVDKKCSIKKNKEEKIRKLFSSFGFMEIDTPCL
                     EYFDMYYGENEYVNQQDIFKLVDKTGNLLVLRPDMTVPACRVAATKIKYETLPYKLSY
                     IAKCFRADEFGGGKQREFTQCGAEILGSGNPYYDASVIKLAINTAKKIGINDISIQIG
                     QVEFFNGLAEKAQLSVKECESLSKMIDNKDSFGISEFLEDKSMEKDVLEHINSITGYF
                     GNKTLIEQLLDTKLNERSAKALENLKNILEILEEMELSKFVTVDLSMVKKLGYYTGMI
                     FNGNTYQMGFPVLAGGRYDNLSGRMGRQLEATGFSLSLEMALKAIERQGIEYDIKKKT
                     VVITFSEKMRKRVLQLDTYTLQAEIIEMLPDSTDIEKYINERNIDELVRCLDDDNIVV
                     VRNNQKTCMTFEKWRKEWSI"
     gene            complement(23284..23964)
                     /locus_tag="PLQ68_05450"
     CDS             complement(23284..23964)
                     /locus_tag="PLQ68_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004842391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015234 - thiamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HXK72129.1"
                     /translation="MKLFFIESDGEFIMTTLGYIAVALLAAIVFAAIFLLKPKKEKEE
                     LKISTTKKLAFCSMAIAVGFVASYLKIFRLPLGGSVTLLSMLFITLVGYWYGLRAGLL
                     AAFTYSLLQFLQGPYIINFWQVCFDYLFAFTALGLSGLFKDRKNGLITGYIVAILARG
                     LFHSIGGYLFFMSWIPDTFPKNLSSIYPLVYNYSYILAEGAITVALLFIPPVRKAMER
                     VKTMAIKE"
     regulatory      complement(24005..24100)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
BASE COUNT         7168 a         5023 c         3537 g         8311 t
ORIGIN      
        1 actcaatagt tgccggtggt tttccggtta tgtcatagac tatccttgtt ataccgtgaa
       61 cttcattggt tattcttctg gatattctgg ataatacgct ttgtgggata tgagcataat
      121 cagctgtcat gaaatcacca gttactactg ctcttaaagc aagcacataa ccatatgttc
      181 tttcgtctcc cataactccg actgtccttg tattagtaag aactgcaaaa tactggctgg
      241 gtttcttttt gagttttcct aattcttctc tatatatata gtcagcttct cttaacatat
      301 ccagtttctc tttggtaagc tcccctatta tccttattgc aagaccaggg cctgggaatg
      361 gctgacgttc agtaatggct tctggaagac caagctctct tcctactttt cttacttcat
      421 ctttgaataa tcctctaaga ggctcgacaa gaccggaaaa tcccatcttt tcaggtaatc
      481 ctcctacatt gtgatgactc tttattgtag ctgctttacc gtttcctgat tcaataacat
      541 ccggatatat tgtgccctga gcaaggaatt ttatacgtcc taattttaaa gcctcatcat
      601 tgaatacttc aacgaattcc gctccaatga tttttctttt cctttcaggg tctgttactc
      661 cttcaagttt tttcaaaaat ctttcagatg cgtcaactgc tatcagcttt aagcttttat
      721 ctgcaaaagc ctcaattact tcctcagctt cattcttgcg cataaatcca tgatctacaa
      781 atatacatac aagctgattg ggtacggctt ttgatattaa ggctgcacat actgcagaat
      841 ccactcctcc agacagtcct aataagactt gctcattacc tacctgcttt cttatttcct
      901 ctattgctgt ttcgatatag ttcttcatgt tgtagtcccc cttggcgtta catatattat
      961 acaggaaatt tcgtataatt gtctttcctt ttaatgtatg tgtaacttca ggatgaaatt
     1021 gaacaccata aagatttttc cccttgtttt ccatggctgc tatcgggcag tctttagtgc
     1081 tggcagtggc tataaatcca tcaggcagtt ttgttattct gtctgtatgg ctcataagta
     1141 ctttaagatc atttcctact ttatcaaaga gaactgattc agaaactaca gtaccacatg
     1201 tttgtccgta ctcactgact gctgcgcttt gaacttcacc ttttagtaca tatgccatca
     1261 tctgcatacc atagcatatg cccaatatag gtattcctga ttccaaaaat tcattgtcac
     1321 acaaaggtga atcaggttta tatacgcttt ttggtccacc agtagttata atccctatcg
     1381 gcttatatga aaaaatttct tttgttgtgg tatcaccaga taatattatc gaatatacgc
     1441 cgagctctct tacccttctt gcaattaaat ctttatactg tccaccaaaa tccaaaacaa
     1501 ttacagtctg cctatccttc atatgtttta ataatccctt ctgcaacttc cttcaatgta
     1561 actcttctat ccatagcctg tctttctata tacttatgag cttgatactc atccatttta
     1621 agatactgaa taagtaccca cttggcatga ttgactaatt tcatctgctc aagttttgct
     1681 ttaagttttt cattagtcct ctcaagtatc ataagtctct cttttgttgc aagagccagc
     1741 ctgagagatt gcaaaaaaac agttctaata acaggttttg cgacaacaag tataccataa
     1801 gattctaatg aataggaaat ttcttcatat gattcgtttc ttactagaac aacaactcca
     1861 cagtcgctgc tttgtgatat gtgttttgct aattctgttc cgaattcatc agttaaggga
     1921 gtattaatta ttactgcatc aaaactgcgg ttcataaaaa gatttcttgc ttcatttgca
     1981 gtttttacat aagttttggc ataaaaagat tcatcacaca atgtgcaatt gaaaaactcc
     2041 atatgttttt cacttgcagc aactataagg atgctgctgg tttcatgcct tgtcatccta
     2101 actcctgttt cagtctttat ttcttctttc taaatagcct tctattatct gtttaggaat
     2161 atatttatcc acaaactcac ttgaagatgc aagtttttta gcatcattaa ggttatcagg
     2221 aagtcgctct attcccttaa ccatatcaca gtttgtttta tacagattca tatccatagc
     2281 atcaggtggc gtcatagcct gcaatatgcc ttctaaagaa gaatagataa gtaatgcgaa
     2341 agttatataa ggattagtaa gcggatctgg agatctaagt tctatcctcg atgctgcttc
     2401 tgatgatgca ggaatacgta taagctgaga cctgtcagag aatgcccatg tcacataacc
     2461 aggagcttta aacttactta atctttgata tgagttatct gaattgttta agaataaagt
     2521 aatttccctt attcttttca atataccagc taccatggaa tctctttctt tagtcgttac
     2581 atcatgtttt gttacagatt ttacagacat atttatatgc agtccgcttc ctgctttatc
     2641 ctttaacggc tttggtgaaa aatccgcata aagtccgttt ctggctgaaa tcgcactcac
     2701 tactgcttta aaagctgtca tattatctgc agctgataac gcatcagaat atgcaaaatc
     2761 aatctcgttc tgccctggtc cctcttcatg atgagatttt tccggtttta tacccatctg
     2821 ttccaaagtc agacagatct ctcttcttac attttcaccc ctgtcatcag gcgctatatc
     2881 catatatgtt ccattatcaa tggggacatt tgtactgttg ccatcttcat ctgttttaaa
     2941 aagataaaac tcgcattcag gaccgaacaa aaaactgtaa ccttgttgtg cagcaaaaga
     3001 aacagcttgt ttcaaaatgt accttgtatc agcaatgtac ggatttcttt tttcatcata
     3061 aacatcacag aaaaaacgca caactcttcc tgatgtcgga cgccacggaa gtaaggacat
     3121 agttttagca tctggtatca gaaaaagatc tgatttagat tcttctttaa atccctcaat
     3181 agccgaagca tcgaaactta tccctccttc aaatgctttt tttagctcgc ctggcataat
     3241 agatacgttc ttctgtcttc catatatgtc acaaaaagcc agtcttataa acttcacatc
     3301 attttgttca ataaactgaa ttgcttcgtc ttttgaataa tacatgtttg ctcctttcaa
     3361 aattacttgt ttattttata ttaatcatga ataacagtaa agttgtttat atggattgtt
     3421 tgtattaggt ctaattacta aaaatggact ttccattatg ttttttatat cctcaccaaa
     3481 catatcacaa tatttttcta tatattcttt tactacttcc atatcttctt catttgcctt
     3541 tgttactgta acctgtgaag gcatgctttt tggcagattc ttggtcctta agaatattaa
     3601 tcctccatgc attcctgtcg cacaaaaatt cccggctact gatttttctt catgtcctat
     3661 accaagcact attattattc ctcctgcctg atactcaccc aaaaaactgc ctactgtacc
     3721 gcctattacc aacacaggaa gtttgttttc atattgtttc atatgaattc cagctctgta
     3781 tcctgcatca ttttttacga atatctgccc gcctctcata gcatatccaa cagcatctcc
     3841 tgtacttcca tgaatggtaa tctgcccttt gttcatggta tctcctgtag catcctgagc
     3901 atttccataa acagttacat ttcctccgtc aagatatgca gccaatgcgt ttccaggagt
     3961 gccctcgata atatattcat tttctttatc cccacaggct atgtacctta cacctaaaca
     4021 gtttgtgtta tccattttta ttacactttt catatcacca cgtctccttt ctgtttaaaa
     4081 ggcttgttct tctttcctgt gccaaagcgc aaagtgcagg ttttttcttt attaaatcaa
     4141 aatccattga acttaaaaga gtcttgttcc ttatcatagc agtgtaaatg cctgcacatt
     4201 ctatatcatc aacgagtata aagcctttta gcatatcatc tttgtaaaac aatcttttat
     4261 atcctttttt gtcttgaaag acatattctt ccccttcata aatgcctgca gtaagaatat
     4321 gaagtcccat aaggcttaac gcgttcatag gcatcccgct taaatatatt ttttcttctc
     4381 cagccatatt tactcctgcg cagaaaccct gcatatatgc atttggtaac agagcgagaa
     4441 tctttttaca gtcagcagtc atgtcatatc cttctgcaca gtctcctgca cagtaaatat
     4501 cttctattga agtagcacaa cactggttta tcagaacgcc tctgttggta tcgacaccag
     4561 ctgctcttaa caaagatata ttcggtctta cgccgactgc catcaccaaa gcatcaaatc
     4621 tcacaacaga atcatctgta agcagtgcgg aattttcata tacctgtttt atactgcaag
     4681 acaatttaag tttcacccct tgtttttcaa tatgttgtgc tacaatatct gatgatgttt
     4741 tatccagtat gcttggtaat acctgttttg ctaaatctat tacagtaacc gatgcaactt
     4801 tatcttttat agcctcacag cattttagtc caataagacc tgctcccagt accagtacat
     4861 ttgatttatc atttatagat ttttccagtt caagagcatc atccaaagtc ataaaagtat
     4921 gcatactttt tactttatcc aacccagata tatccggaac aaaaggaagt gaacccgttg
     4981 caatcaaaag cctgtcatat tcataagaag aagaatcctc aagtatgaca gccttcatgt
     5041 cagggatgac ttttactgct tttttcccaa ggactacagt aaccgaattg tcttcataaa
     5101 aactatcagg acggtatttc atcttatcaa aatcggtttt accctgtaaa agatatgaaa
     5161 ttaaaggtct tgaatatgta tgatggtttt caatagatat taatgtaatc cttccttgct
     5221 tatcaatact ccgtattcct tcaattgcag caatagctgc tgtggaatta ccaattatca
     5281 catagttcat cctacacctc caccagttct atagccttat taggacatgc tgatacgcat
     5341 gcaggagagg aaactgaatt acctacacat aattcacatt ttctcactgc tccttcttga
     5401 gatggcataa ctgctccata tggacatact agcacacagg tataacagcc tatgcatctt
     5461 ttcttatcaa tagatacaac gttgtcaatt atactaagtg ctcctgttat gcagcttttt
     5521 acacatatag ggtcttcaca gtgacggcat gaaaccgcaa atgatatctt atctttctgt
     5581 tctattctca gctttggctg aatcatcatg tctttcaatg cttttaccat atcctcaaca
     5641 cctgaattag caaatgcgca gtaatattca caaagatgac atcctaaaca ccatttctca
     5701 ttaactttaa tcattttcat atcattcacc tgcatgccta acgcctaata tctcaagctc
     5761 tttttcattt agtccgattc ctcttaacat taacctgttt cctcttaatg cttctattga
     5821 attaattccc attcccccca tcatttcttt aatctcatgc gtccatgcat tgattaaatt
     5881 aacaagcctt tcataaccaa tttcaggatt gagtctcttt actaaatctt ccctctgggt
     5941 agcgatgccc cagttacatt ttccgctata acaagtacgg cacaaatgac atcctaatgc
     6001 taaaagtgct gcagttgcta tatatactgc atctgctcct aaggctattg ctttaaccac
     6061 atcagctgat gagcggatag agcctcctac aaccagcgat acattatccc ttataccttc
     6121 atttctcaat cttgtatcaa cagatgccaa agctagttca ataggtattc caacattgtc
     6181 ccttatcctt acaggagctg ctcctgtccc tcctctgaaa ccgtctatcg ctatgatatc
     6241 tgcacctgat cttgctatac ctgaagctat tgcagataca ttatgtacag cagcaatctt
     6301 aactattaca ggttttttga aatcagtagc atgctttagc gacataacca gttgatttaa
     6361 atcttcaatg gaataaatat catgatgagg agcaggactg atggcatccg tgccaaccgg
     6421 tatcatcctt gtttttgaga catcttcagt tatctttgtt ccaggaagat gtcctcctat
     6481 tccgggtttt gctccctgtc ccatctttat ttcaacagca gctcctgcgt taaggtagtc
     6541 cttatgaact ccgaatctgc ctgatgcaac ctgaactata gtattacttc cgtactggta
     6601 gaagtcttca tgaagacctc cttctcctgt gttatatagt attgataaac tttttgccgc
     6661 tctggctaaa ctttcatgag cattatagct taaacttcca taactcattg cagaaaacat
     6721 tataggggta gaaagctcca attgaggtcc aaggtttgat attattctac cttcattatc
     6781 ccttttgatt tcatgttttt tctgccctaa aaaagtcttt gtttccatag gctctctaag
     6841 aggatctatt gatggatttg taacctgaga agcatttata agaagtttat cccaataaac
     6901 aggaaatttc attggattgc ccattgaaga aagaagaact cctccgctcg atgcctgctt
     6961 atatacatca gttattatct ctgctgtcca gtttccgttt tctttaaaac tatcctgatt
     7021 tttaactatt ttcaaagcag aagtcggaca aagtgcaaca catctgtgac agttaacaca
     7081 ttttgattca tccgcaacca taattttata gtcttcatca taatgatgga cttcattagc
     7141 acactgtctt tcacagactc tgcactttat gcatcttgac tggtctctta ctatttcaaa
     7201 ttgtggaata atatagttta tgggcatcag cttatacctc ctcgaagtgt atatattact
     7261 ggctctccgc ctcttgggga gtaaacagaa tctaaatcct tacatatttt ccttattgct
     7321 gattcttcag aagatatata aacagcaggt cctttctccc ctacaaccat agaacgaagt
     7381 ttaagtctgt catttaatgc cataatgcca ccactgaaac ctgcaattat ggaaaaaggc
     7441 ccagttatta aaagtgaaga aaaagtattt cttagatatg taagtctttc tttttcttct
     7501 ttgtcttttc tgtctattgt tgaccaaaaa ggggcggcaa taacacttgc agcttctgaa
     7561 tatgtcagcc caagcttacg taccagaaaa tccagtatat atgctattac ttctgtatca
     7621 gtcagcaaat tgcatttata tccgaacatt tcgatatatc tgcgattagc gtcataagaa
     7681 gaaatttctc cattgtgtac aattgagcag tcaagaagcg aaaaaggatg agctcctccc
     7741 caccaccctg gagtatttgt gggatatctt ccatgagcag tccagcagta tgcgttatac
     7801 tcatctaatt tatagaagct tccgacatcc tcaggataac caacagcctt aaatactccc
     7861 atatttttcc cgctggagaa aacatatgct ccttctatat cagtattaat tcttgttaca
     7921 cataatgatg tatactcctg ttcatcaagc tggcttgaga aaaggtctgc tggatgaggt
     7981 aatacaaaat atctgaaaat taaaggttcg ttaacaatct tcggatgttt ttttgtaggt
     8041 atcctcgaaa gattaactac atcaaagtgc ttattaagat actcttcaca tgttactttg
     8101 gcacggtcat catcatagaa tatatgaaaa gcataatatt ctttatactc aggataaata
     8161 ccataaccag caaatccgcc acctaaacca ttagatcttt catgcattat ggcaatagcc
     8221 tgaataatgt cagaaccgct tattctcttt ccatctctgt tgaatatgcc gcatatcgca
     8281 caacctgagg gtattctgta attgtctgtt ccttctcttt gcataaaatt ctccttaaaa
     8341 ataaagacga ccgataagaa agcctgctgg cttcattacc ggacgtcttt gtcctttaaa
     8401 ttaatattat agaaaattga cgacttgtca actctttttt tgtcggtata ccaaatctct
     8461 tacaaatttt tcatattttc gataatgaaa tttgatagcc aattatggat tttaaaattt
     8521 taaaaaatat attgacatga caaatttggg atatataata aatgagtaga cagcaaaggc
     8581 gctgtgagta cagtactcgc agcgtctttt tttattgaaa aaaaggagga ttacagatgg
     8641 aatatactac acatgatgta ccaaaattat tcggaagcgc agtatttaat gatgctgtta
     8701 tgagaagccg tcttccaaag gatatatatc attctttaaa aaagacgatt gaagaaggaa
     8761 ctgatttaga tataaattca gcaaattccg tagcttcagt tatgagagac tgggcaattg
     8821 aaaaaggtgc tactcatttc actcactggt ttcaaccgct taacggtgta accgcagaaa
     8881 aacatgaaag cttcttatca gcccaatcaa atgggactgt tattatggag tttaatggaa
     8941 acgaattaat aaaaggtgaa ccagatgctt ccagttttcc atcaggagga ttaagagcaa
     9001 cctttgaagc aagaggatac actgcatggg atccaacatc atatgctttc attaaaggaa
     9061 actgcctatg catacctaca gttttctgtt cctactgcgg aagtgttctt gacaaaaaaa
     9121 caccgcttct tcgctccatg gaacagttaa acgaacaggc attgagaata ctaaaacttt
     9181 tcggacatag tgatgttact catgtagcct caactgtagg tccagaacag gaatatttcc
     9241 ttatagacaa ggaaatgttt gaaaaaagaa aagatttgaa attctgcgga cgtactcttt
     9301 ttggagcaaa acctccaaag gggcaggaaa tggaagatca ttatttcggt gctataaggc
     9361 caagagtgga agcatttatg caggatttaa ataatgaatt gtggaaactg ggtatattcg
     9421 ctaaaactga acataatgaa gtagctccaa gccagcatga attagctccg attttcacaa
     9481 ccactaatac cgcaactgac cataatcaga taacaatgga aatattgaaa agaacagcag
     9541 aaaaacataa tatggcctgc ttattacatg aaaaaccctt tgcaggcata aacggttctg
     9601 gtaagcataa taactggtcc atgagtacca atacaggtaa aaatctttta gatggaggaa
     9661 aaacaccttc atccaataaa cagtttttac ttatcttaac tgcaattata aaagcagttg
     9721 atgagcatca ggaccttcta agaatttctg ttgcttctgc aggaaatgat cacagacttg
     9781 gagcaaatga agctcctccg gcaatattat ctattttcct tggagaagaa cttacaggaa
     9841 tacttgaaag tctagctaat gatttaatat ataacggcaa tggccatgtc agtatgaaga
     9901 caggtgttca tgcaataccg ggatttagaa aagatactac cgacagaaac agaacatcac
     9961 cttttgcttt cacaggcaat aaattcgaat ttcgtatggt aggctctaat atgtctattg
    10021 cagatgccaa tattgtctta aatacaataa ttgctgacag tttatcatca ttagcagatg
    10081 aactggaaaa agctgatgat attgataaag cagtagatat gcttataaag aaaacatatg
    10141 tcgatcacag aagaatagta ttcaatggaa acaactatgc gccagagtgg gttgaagaag
    10201 cagaaaaaag aggtcttttg aacttaaaga ctaccgctga ttctctttct atgtttatat
    10261 ctaaaaaaaa tattgagtta tttgaaaaat acggcatact gagtgaaatt gaacttcgtt
    10321 caagatatga gatactttta gaaaactact gtaaaactat taatattgaa gctttaacca
    10381 tggctcaaat agctaaaaga gatatttatc cttctgtttg cggatatctt aaaacattgc
    10441 ttgatttact aaattctaaa aaacaagctg gtattgaatc atcttgcgat tccttattat
    10501 cacacatcaa aaagttatca tctcttagtg atagtctgta tggagaaata tcagagcttt
    10561 tagatgctgt tatgaatcaa aacgaaataa gcagcagtca tgagcttagt ttttactgca
    10621 aagataccat aatacctatt atgaacagat taagagccgt tgctgatgaa cttgaaacac
    10681 agacatcttc agatttgtgg ccatttccaa cttatagtca gatactttat tcagtgtagt
    10741 aatttcagtc tagtacaaaa agcagcacct gaaggtgctg ttttttttat tcttttactt
    10801 gtttttattc ttttacttgn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt
    10921 attcttttac ttgcttatat tcatttactc aaattctttc tcttacatat acctcttttt
    10981 tctatacttt attctttact tcttcagtac tccaataagg ccttttataa cattaccatt
    11041 aagcatagta actattgatt cgactttttc atgagtaaac ataccacctg tcatagtact
    11101 taatgatctt aagggcatat catatatcat accttctatc atctttttca tactgtcacc
    11161 atcttttgtc atatccttca tgattttttt agcataacct tcaagcagct ttaccagaag
    11221 tcttcctata aatgaggtct gtatatcttt tattgtacta tttatagtat atggtctgtt
    11281 tgagggttcg tttggaattt caaaatctaa aacagctctg aactgattat ctggaatatc
    11341 cagagtttct ttacctaaag cataatatgc aggagcgaca gatctcatgt ccaacgaatt
    11401 aatatctctg tttccttcaa tttccacctg agcagataaa tgaatatttc ttgatgaaga
    11461 agctatctca actgtataaa tcccgctttc cacatgccag tctgcactgt atgtattgaa
    11521 gtaagcaaat gctcttttat ccagggtaaa agtaacagtc ttctcctgag ttggttttaa
    11581 aacaactttg gaaaaacctt tcaattctct tattggtctg aatactattt ctgatgatga
    11641 cttaatatac agctgagtta tttcttttgc ttccatatgt cctgtatttt taattttaaa
    11701 agacacattc aatgtctcat catctttcat cttgttttta tccagcttga tatttgaata
    11761 tgtaaagttt gaatatgaaa gtccgtatcc gaatggaaat gctacttcct tatttgcttt
    11821 atcatagtac ctgtagccta caaagatact ttctcgatac tctgcattct ttttcttggc
    11881 aaaccatcta tatgacggat tgtcctcaag tgatttcaca aaactttcag ctagttttcc
    11941 acatggactg taatctccaa aaacaaggtc atatacagca cctccattgt tttgtccagt
    12001 cagatacatc ataatcgcac ccttgaccag tttcatccat ggcataacta ttgctgctcc
    12061 tgaatatatt atggctattg ttaacctatt ggcattgcat acgctttcaa taagcatatt
    12121 atgagatggt ggtagctcca tatgagttct gtcataacct tctgactcat aagcatctgg
    12181 aagacctaca aaaacaattg ctatatctgc tttttttgcg gtttgcgcag cttcagaaat
    12241 caactgcatg tctggaacat cactatttgc gtcataacct ctcgcatact cgaaattaat
    12301 acccttattt gcaaactctt caaccacact agtgactttg tatgatgata catgtgagct
    12361 accggctcct tgataccttg gtttttttgc aaactcacct ataattgcaa ctttagtatc
    12421 ttttttcaaa ggaagaatat cattgttctt aagcaatacc gcgctttctc ttgcaatacg
    12481 tcttgcaagt tcattgttcg catccatatc gcaattttct ttttcaacat cattcgaagc
    12541 taatgctatg gctaccattc ttgcagcact tttatttagt gcttttatat caagcaaccc
    12601 gtttttaacg gcactaataa tcgctttttc attttcagtt cctgaatatg gcatttccag
    12661 atccagtcct gcacttactc cttctaccct gttattaact gcaccccagt cagatacaac
    12721 tgcaccttta aaaccccatt catctcttaa aatatcattt aagaatctct tgttttctga
    12781 tgcatatgtt ccattaattt tgttatatga acacatcact gtccatggct gggcattttt
    12841 aacagcatcc tcaaaagcag gaagataaat ttctcttaat gccctttcat ctactatgga
    12901 atcagaaacc agtcttagtt tctcttgact gttagcaaga taatgtttta tcgatgttcc
    12961 tacattcttg ctttgaacac catttatgta cgcagttgcc atttttccgc ttaaatacgg
    13021 atcctcactg aaatactcaa agtttctccc gcataaagga gatctcttaa tattcacagc
    13081 cggtcctaac actattgata ctccctgatc taggcattcc cttgcaacag cagctcccat
    13141 ttcacctatt aattcaacat caaatgaaca ggcagtagta catgctggcg gaaagcaagt
    13201 agccggatat gattcattta tacctaaatg atctgattca tctttttgct ttctcaatcc
    13261 atgcggacca tctgtcatca ttatagatgg tacagaatgc tcattcacct cttttgtatg
    13321 ccagaaatcc ttgcctgaac acaaactggc tttttgctcc agactcattt tagtaactat
    13381 cttctccgct ttttcaacat attctgcttt tatcgcatag ttagtcattt ggtatacctc
    13441 ccttcaagta actggtcagc ttgttctttc gttatattcc cctgattata ttctgccatt
    13501 atctgtacat ccttgctcct taatttatac attctcagga ctggaagtcc taatatgacc
    13561 cctattactg gaaatagtga aaacattacc cactccccat acaatgtggc ttctgactgt
    13621 actacattca atccttcctt aaacccgaac catcctaaaa ccagcattgc aactgctgta
    13681 gttacagcgc tcataagttt tatagtaaat gtctggatgg aaaaagttat cccttctgcc
    13741 ctttcatttg tcatgtatgt tccatactct acacaatctg gcgtaaacat gtataacaaa
    13801 atggtaaatc ctccgataca tattcctttt aaagcaataa atatgtaaaa agcagtaaga
    13861 ttctcatatc ctacaaaata tgatattatg ctgaacactc ccataccagc aattgaccag
    13921 aaatatatat gatatttatc tatctttcga gtaattgaag gtattaataa agctattatc
    13981 aaagcaggta caacaaagaa tatgctcaca attgtagcca ttgattcatc ttttaagcag
    14041 tctcttgcaa aatatatagc tacaacctga gaactgtttg caaatgctat tagaatatat
    14101 gaaagataaa atatgagaag gtatttattg ttttttacat accttatcat ttgtttgtat
    14161 gtaatctttt cctgctttct aactttaact ctttcttttc ctatgaaaca taagggaatc
    14221 ataaatataa atcctgctat tgttattatg gctgctgtag ctgtccatcc aagtataggc
    14281 cgaatggttg gaagaagaac aacaactgcc atagagccta tcgtgcccat aaatcttcct
    14341 attgatagga tagcggtacg ttcgtttatg ttatctgtca tagatgttga caacgcaaat
    14401 atcggcacat cacaaaccgt ataagctatg tcccataaga tatatgaaac aaccgcccag
    14461 attatctttg cgacaggtga agcacttata ggcataaaga atatgaacat tgttgttgct
    14521 gctataaaga caagtgaaat tcttatccat ggcatgaatc ttcccttttt aaacctagtc
    14581 ctgtctacga ttattccaaa aataggatca tttacagcat cccatatctt ggtaacaaga
    14641 agaacaatgg ctacagctga tgcagtaatg cccatatcag taaaatatac ctgaataaac
    14701 atagcgacaa gcacatagaa gaaattctgt ccaagaaaat aaagaccata actaaatctt
    14761 tccgataaag acgacgaata atcgcttctt gaccccataa aagaaatcct cctcacatga
    14821 caataagcca ttcatacaaa taacattaat gtattgtata atatatgtta gttataaaat
    14881 atgataaatt gttgaaaatg tcaacaatat cgattttaaa gcataaaaaa aaccgccttt
    14941 tgagacggtt tcattttttt attgatttta tccttctata gaaatattct tttcttcttc
    15001 tttaaagaat tcctcatagt aagcatttct ttcggcttcc tttattttgt ttgctttata
    15061 gaaaaggtat actgaaggaa taaggaatac tacacctact acgaaccaaa cccacatttg
    15121 ccatcttgcc atatatttct cattcatgta aacgagtctc atgaataatt caaaagaaat
    15181 agcttcaaaa cctattgcga atagtacaat tgctaatttc attacttttg aactcttgct
    15241 gcctttttca tccattaaat atatacctcc tgcatgtagc attaacatac gatttacttc
    15301 ataaagtata tcattcgata tatgcaatgt caaacgattt gaaccgtttt taaatctttg
    15361 tggaaattgc ataattccat gttttaaaat atatataatt tactaaattc taacaggaat
    15421 acctttattt ttcaattcct gcttaagctc tattatatct aattcatgaa aatgaaacaa
    15481 agaagcagct aaagccgcat cagcttttcc aattgtaaga acatcataaa aatcatttat
    15541 actcccagca ccacctgaag caataacagg aatgcttaca gcctctgaaa ccattgctgt
    15601 cagtgacaat tcaaaccctt tttttgttcc atctgcatcc atggaagtaa gaagaatttc
    15661 tcctgcaccc agtttttcac attcttttgc ccattccaca gcattcttag aagtcttatc
    15721 tctgcctccg tttatatata ccatataact gccatcctct tccctttttg catcaattgc
    15781 agctacaaca cactgtgaac caaataattc gctggctttt gagataaggg aaggattcaa
    15841 gattcctgct gtattaacag aaactttatc agctccagct cgaagaagca cattaaaatc
    15901 atctgttgtc cgtatccctc cacctactgt aaaaggtata aaaacttcag aagcaacctt
    15961 ttctaccatg gataaagtta tatcacgttt ctcatatgat gcagttatat caagaaaaac
    16021 aagctcatct gcaccttttg cgttatatgc ttttgctgtt tcaacaggat cgcctgcatc
    16081 tattaaatct aaaaaattaa ttcctttaac tactcttcct ttattgatat ccaaacaagg
    16141 tattattctt ttcgtgtaca ttctcttctc cattctttat ctttatttaa aaagactttc
    16201 caaacttatc ttaccttcat atattgcttt accagttata acagcataag ctcctgtctt
    16261 tttaacttct tctatatcat caatatcctt aattcctcct gatgcaacta catcaagacc
    16321 tgtttttaag atcatttctt tcaaagctat tatattaggc ccttctagcg taccatcttt
    16381 tgaaatatca gtatatacga ttgaagatac tcccatatct ttcataatca tggctaaact
    16441 aattgcattc tgatcactgc ttgatgtcca cccgtctaca gaaactttgt catttaatgc
    16501 atcaattcca acaactattt tttctttata ctcttttact gccaatctta caaggtgagg
    16561 gttttttatt gcagcagttc ctaaaacaac cctgtctgct cctgcactga taagttcttt
    16621 gattctttcc atggttcgga taccgccacc agtctgaacc agaagattcg tattggaaca
    16681 aatgcttttg attatctcag tattgacaga caaacctgtt ttagctccat caaggtccac
    16741 tatatgcaga tgagtcgctc cagcattcat aaacgcgata gctacttcca aaggattctt
    16801 tgaatactca gtgaccttgc tgtaatcgcc tttaaatagc cgaacgcatt ttccattaag
    16861 tatatcaatt gccgggtata ttatcattgc tcaacccact ttctcaataa atttatgccc
    16921 tcgtctccac ttttctcagg atgaaactgg catgccagta tgttatcttt ttcaactgct
    16981 gaaggaaatg ttataccata ttgagtcaat gcataatcct gctctgaaga attctctata
    17041 cttgcataat atgaatggac aaaatagaaa tacttttcat caaagtcttt aagtatcctc
    17101 atattctcat ttgtctttat actgttccag cccatctgtg gtattttaag agatatatct
    17161 tctctgaatt tttttacgtt cccattaagc agtcccatgc ctgtaacaag atgcttatct
    17221 tctgaaaact cctcttcaga tgtttcaaat aaaagctgat aacctaaaca tataccaaga
    17281 aatgttttac cttttttaat ttgttcggta actgcatcag ccattccttt actgtttaag
    17341 ttttccatag ctttttcaaa agccccaact cccggcaaaa ctatcttgtc atacttgttc
    17401 agcaaatcag gatcatttac tataaaaact ttctgtccaa taaaggaaaa tgctttaata
    17461 atactcccaa tattacccat tccataatct attacagcta tcactttacc agcctttcat
    17521 atgcctgcat atacttattg agagtctgag ttatgacttc atcagtaagt tttggtgcag
    17581 gtggattttt atcccatccg gtagtttcta aatattccct caaatattgc ttatcaaaac
    17641 tcttttgtgc ttttccaggc atatagtcat caacatccca gaatcttgaa gaatcgggag
    17701 tgaatatttc gtctgcaagt acaagcctgc catcaagtac accgaattca aattttgtgt
    17761 ctgcaattat tattcctttt gtcaatgcat aatcactgac ctttttgtat agttccagac
    17821 tctttttttt cagcttaaga gctaagtcag ctccaatcat ttttacagtc tcttcatatg
    17881 atgtgtttac atcgtgacct tcttcagctt taatcgacgg tgtatatatt ggttcatcca
    17941 gtttatcagc ctgttttaat ccttctttca aaggtatatt gcatacactg cctgttttgt
    18001 tgtattcttt cagtccgctt ccttcaagat atccccttac tacgcattca accggaagca
    18061 tttttatttt ttttacaagc atggaacgac ctttgaaata ttcatcttca ttaaaaggag
    18121 cagggaaatc tgaaatatca gcagatataa ggtggttttc aataatatct tttgtatatt
    18181 caaaaaagaa tgctgatatg ctgttcaata ttataccttt tgttggtata ggttcatcaa
    18241 aaacacagtc aaatgcagat atcctgtctg atgcaaccat aagcatttta tcgtccaaat
    18301 cgtataaatc cctaacttta ccttttttaa atggtttgat attcattaaa tctctccttt
    18361 tgttgatggt ataccagttt cattttcatc tattttaaca gcttgtctta aacttcttgc
    18421 caaggcttta aagatacttt cagctatatg atgaagattt gatccatata atacatttac
    18481 atgaatattt gctccaagat tatttgaaag agctactata aattcttcta tagtctgaaa
    18541 atccatttcg ccaatccttc cctgcttaac aggacagttg aaaactgtat atggtcgtcc
    18601 acctaaatca actgctacag atgtaagagt ttcatccatt ggaagaagaa tatctgcata
    18661 tcttgtaatc ctgctcttat caccaagagc cttctttata gtctcaccaa taacaattgc
    18721 agtatcttca actgtatgat gacagtctac aaagacatca cctttagcaa ccagtttcat
    18781 gtccatcagt gagtgctttg aaaaaagttc cagcatgtga ttaaaaaaac ctatttctgt
    18841 ctctatctca tattggcctt ttccgtcaat atttaattct aaaaagatat cggtttcttt
    18901 tgtctttctt ttagtaactg cttttctcat tttttcatta tctcctttgc caaattaata
    18961 agaatatcca tatcactatc tgtacctatg gaaattctta catagtcaga tatgtaggaa
    19021 aactgcctga taagtatacc tttatctcta aattttgtat atatagtata tgagtcaaat
    19081 cctggaaaag aaacaaaaat aaaattgctt tttgaaggaa gaacaacaca tcccatctgt
    19141 tccaaagctg tttttgttct ttctcttgtg tctattacca tttgatttat attattataa
    19201 tatgtctggt ctgtaacagc ttctttcgca cttatctgtg caagcctgtc taaagggtat
    19261 gagttaaaac agtttttaat tgtctttaat gcatctataa gcaatttact gccaattgca
    19321 taacctattc tcattccagc taaacaataa gatttcgaga atgtttttac tacaagaaga
    19381 ttctcattat ccagcacata tggtaccatg ctctgttgct ttgcaaaatc aatgtatgct
    19441 tcatcaacta aaacaagatt atccctattt ctcttaagta atgaaaggat atattcactt
    19501 tctaaaagtt ctcctgttgg agcatttgga tttgctacaa ttatgggaca atccatttca
    19561 ctcaaagcct ttttatctat catatatcca tctttcattg gtactgttct ggctttacaa
    19621 ttatacaaac tacaatagac cggataaaaa ctatatgtga tttcaggaaa ggctacatcc
    19681 ttgtttgcgt aaaatgcatt aaaagcaaat gctaataatt catctgaacc gttagacaca
    19741 aatatattct ctttacctac gccatgaaaa tctgctaaag tttcaataag atcgatactt
    19801 tcggggtcag gataaagcct taaccctgaa ttagcacctt tttttattgc ttcaataacc
    19861 ttaggtgaag gaggataagg gttttcgttg gtgttaagct taatatacct tttatcattt
    19921 agctgttctc caggtgtata tggagataat ctattaagaa catcagaata tatgttactc
    19981 attttcatca ccttcaaacc ttatatccac tgcattggca tgagcatcta aatcttcaca
    20041 ttttgcaaaa agcgatatgc ctttatatgc attatttaaa gctttcttat catattgtat
    20101 tatgctgctt tttttcatga agtcatatac tcccagaact gaaaagaatc ttgcagttcc
    20161 cgctgtaggc aaggtatggt tcggaccggc gaaataatca cctaaaggct caggtgaata
    20221 tggtcctaca aaaacggctc ctgcctgagt tatgaaatca gaatagaagt ctgcatcctt
    20281 ggtcataatc tgggcatgtt caggtgcaca ttcattcagc acttcaatac attccattat
    20341 atctttccct acatatattg cgctgttatc ttctatgctc ttttttataa tatcctttct
    20401 tttaagcttt tcagcctgta taacaatctg gttttgaact tcatctgcca gctctttgga
    20461 cacagtaagc aaagaagcgc aggaaagctt atcatgctct gcctgactga gcatatctgc
    20521 tgcaataaat cgcggatcag ctgtttcatc agcaattatc attatctctg atgggcctgc
    20581 aaccatatct ataccgcaat atccatatgc catctttttt gcatatgcaa cataaatatt
    20641 tcccggtccg gttatcttat caacttttgg gattgtctga gtgccaaaag ccatggagaa
    20701 gattgcctga gctcctcctg ctttgtatat ttttgtaaca tgcgcttcat tagcagcaca
    20761 aattctggca gcatcaatta caggaggcgt acacattaca atctctttta cccctgcaac
    20821 atatgctggc actgcattca tcaatactga tgatatcaat ggagcagttc caccaggaat
    20881 gtatatacct gcacttttta gaggtgttac cttctgtcct atacttatac cctctgtctt
    20941 attccataaa aaactgtctt ctttctgttt catggagtaa cttgcaatat ttattatcgc
    21001 ctgctgaatt attttaagaa gctctttgtc aataatttta tatgattgct caatttcctc
    21061 ctttgtaaca ataagctgat ctgaattaag tgtgatatta tcgaattttt ttgtatagta
    21121 aagcaaagct tcatcaccct tagtccggat atcctccata atttgaaaga ctttatcata
    21181 tacttcctta ttatctaaaa atccatcttt tctgactttg tttataaaac ccagtttatc
    21241 attcctataa agcggtatca tttcttaact ccttcttcag cgcttgtatt atatacgata
    21301 tcttactcat tttcatattc atgcttacct tgttgactat taggtgtgct gatatatcgc
    21361 atattttttc taagaccata agaccgttag cctgtagcgt ctttccgctt tccactatat
    21421 ccactatcag ttcagacaga ttacataatg gtgcaagttc tactgaacca tttaatttaa
    21481 ttatttcaac attttcattt ttaacatttc tataaaattc acttgctatc ttgggatact
    21541 ttgtagctac tcttttgttt attatttcgt ctgtcttacc aaagtatttt tcttctcctg
    21601 caacgcacat tctgcaggct ccaaaaccaa gattgagaac atcataaaca tttcttgatt
    21661 cttccataag agtatctctt ccgactacac ccatatctgc agcaccatat tccacataca
    21721 caggaacatc agcagctttt actaaaaata ttttaagctt attttcttca tctatatgaa
    21781 tcagctttct tgaatcttca tcaatattct tacatggtat acctgatttt ttaaaaaatt
    21841 caatagtctg tatagcaagc ctgccttttg gcaaagctat atttaaatac tccattcttt
    21901 cctccacttc tcaaatgtca tacatgtctt ttggttattt cttacaacaa ctatattatc
    21961 atcatcaaga caccttacaa gttcatcaat gtttctttca ttgatgtatt tttcaatatc
    22021 tgtactgtca ggaagcatct cgataatctc ggcttgcaaa gtatatgtat ccagttgcag
    22081 tactcttttt ctcatttttt cggaaaaagt tattacaaca gtttttttct tgatatcata
    22141 ttctataccc tgcctttcaa tagctttaag agccatctcc agacttaatg aaaaccctgt
    22201 agcttcgagc tgccttccca tacgaccgga aaggttatca tatcttcccc ctgctaaaac
    22261 aggaaatccc atctgatatg tgtttccatt aaatatcatc ccagtatagt acccaagttt
    22321 ttttaccatt gacaaatcaa ctgttacaaa cttgcttaat tccatttcct ctaaaatttc
    22381 tagtatattc ttcagatttt caagagcttt agctgacctt tcatttagtt ttgtgtctaa
    22441 aagctgttct attaaagttt tgttaccgaa atagccagta atagagttta tgtgctcaag
    22501 aacatctttt tccatgcttt tatcttctaa aaattccgat atgccgaaac tgtctttgtt
    22561 atctatcatt tttgacaaag actcgcattc tttaactgat aattgagctt tttctgctaa
    22621 tccgttaaag aactctacct gccctatttg gattgatata tcatttattc ctatcttctt
    22681 tgcagtatta attgcaagct ttataactga cgcatcataa tatggattac cgcttcctaa
    22741 tatctctgca ccgcactgtg tgaattctct ttgctttcca ccgccaaact cgtccgctct
    22801 gaaacatttt gcaatatatg aaagtttata tggtaaagtc tcatacttga tttttgttgc
    22861 agcaaccctg catgcaggaa cagtcatgtc tggtcttaat actaaaagat ttcctgtttt
    22921 atcaacaagc ttgaatatgt cctgttggtt cacatattca ttttctccat aatacatgtc
    22981 aaaatattct aagcaaggag tatcaatctc cataaaacca aagctgctaa ataattttct
    23041 tatcttctct tctttattct tttttattga gcacttttta tctacgatgt cgttcatgcc
    23101 ctctgggttg tgaaaaacca tgtctgtctc ctttgaactt tgctacacta aagcgttaaa
    23161 ttgctaaagt gttaatatct tatcatcatc tattatacaa tgtcaaccaa aaaatgaaaa
    23221 aaaaccgggg aaatttccaa cccggttaat tagtaatcaa tatatcaatt catattgaac
    23281 atcctattcc tttatagcca tagtcttcac tctttccata gctttcctta caggaggtat
    23341 gaataatagt gctacagtta tagcaccctc tgcaagtata tacgagtagt tgtataccaa
    23401 tggatatatt gaagaaaggt tcttaggaaa agtgtcaggt atccatgaca taaagaacag
    23461 atatcctcca atcgaatgaa acagccctct tgcaagtata gcgacaatgt aaccggttat
    23521 gagtccgttt tttctgtctt taaataatcc ggataaacct aaagcagtaa acgcaaagag
    23581 atagtcaaag caaacctgcc agaagttaat gatataaggg ccttgtaaaa actgtaaaag
    23641 actgtatgta aatgcagcta aaagacctgc acgcagacca taccagtaac ctactaaggt
    23701 aataaacagc attgacaata aggtaactga accacctaaa ggcagtctga aaattttaag
    23761 ataggatgca acaaatccaa ctgctattgc catagaacaa aaagcaagtt tttttgtcgt
    23821 actgatcttc aattcctctt tttctttttt tggcttcaaa aggaatattg cagcaaaaac
    23881 aattgcagcc aacaatgcga cagcgatata tccaagtgtt gtcataataa attcgccatc
    23941 tgattcgata aaaaatagtt tcataataaa atacctccgt tattcatata tgcaggaggt
    24001 aaaatggttg ttgtcagtgc ttccttacga cggtattatc cgtatcaagt agtgagggtc
    24061 aatcatgttt ctcagccttt gggctcccct agcatatata ttgaattttc aaattgccgg
    24121 taataccagc agcattatt
//
LOCUS       DAOSXM010000028        22464 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2111
            NODE_1457_length_22464_cov_9.117274, whole genome shotgun sequence.
ACCESSION   DAOSXM010000028 DAOSXM010000000
VERSION     DAOSXM010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864178
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 22464)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22464)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:24:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,261
            CDSs (total)                      :: 2,229
            Genes (coding)                    :: 2,218
            CDSs (with protein)               :: 2,218
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 3 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22464
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1457_length_22464_cov_9.117274"
                     /isolate="AMR_MDS_2111"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..569)
                     /locus_tag="PLQ68_05455"
     CDS             complement(<1..569)
                     /locus_tag="PLQ68_05455"
                     /inference="COORDINATES: protein motif:HMM:NF014038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HXK72130.1"
                     /translation="MINIRKNTETELKVMITKDEYNTFFSKAHNIYSQTNHYYKLNDN
                     LCVRIRKINDKFFIQYKESNNTKELKALKDKTEYSMDISETDYIIINNNPNCLYDYLG
                     LKNDNKIKAEYKGTLETIRGHVTLDLKMPDMQIDLNKYNSNIDYELEWEIEKKQYKKA
                     INILKKNNVDINNRITGVSKLKRLLESGI"
     gene            complement(601..4050)
                     /locus_tag="PLQ68_05460"
     CDS             complement(601..4050)
                     /locus_tag="PLQ68_05460"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HXK72131.1"
                     /translation="MVKWTEQQQQAISLRNTDILVSAAAGSGKTATLIERIISILSDD
                     ELGTECENLLVTTFTNAAAGEIKERVRKALKEKILQIPENEYLKKQYRNISRATISTL
                     HSFCSEVIRNNANLINIDPNFSVVDETIADNYLEDAITSVYKNKLQNKEENFLKLLST
                     LGYGKNLDTLTSLIISILKTVKSYPDYEQWLTKKTEESVIKHELIFNSKYGIEIKKHI
                     LRKLNSCEIYINNISSILSGTVEYCHYEDMLNHDFNILKNIYDFIETLDWDALKEYFY
                     DLKYISLPRKKKDFNEEYVDAIKEYRKAFKNSIIDITSQIYDDTENIKKDCEIMYPIL
                     KEFTGTLLCVDKAYSEKKSKAKVIDYSDMEHMALKCLKSGAYELYRDKFKHILIDEYQ
                     DFNYIQEEIINQIRREGNVFAVGDLKQSIYRFRNAQTAIFRNKATQFQKSTEKGMKLN
                     LSYNFRSRNTILEYVNSIFSNIMNDNLGEVEYSEEEKLRYGNYNFDKDSSEDIYLPKI
                     LKYEYSKDLYNEFPTMTEIEVEAFVIVQEIKQLINSGITIFDSNKKDTRAIRYSDIVI
                     LLRSVNPYAKKYCEILNKCGILAYSDQSVEYMNDKSISDVYALLNVTDNPYQDIYLAQ
                     VMKNKVYAFNEKEITKIKKSRYGLYNDLINYKENKLLENNELLECKIKRLIDDLTAIR
                     NFAKVNTLEKILFYAMTKSGFYYVSENQELLKRLYALAVECHKKGINDVSGFLNFIKS
                     KKKKDKDIISEILKTQSLDSVNVMSIHKSKGLEFPVVILAGCSRKFNIEETKNRLLID
                     RDLGFGIEYISDINKTYHKHITKNALSLSIKRQNLSEEMRILYVALTRAKEKLIMVGK
                     EPDKKDVLIADDAKNYFDWILPNSDNSIQNIIKASEFSAVISSYISESKQQTKKEENF
                     DKTAYFQDIMARLGYEYPYIEETTLNRKISATELAKYKSKNYDIEEFYNQYKTTVKKR
                     PEFLNKSGSKQNAAEKGTMMHKIMASIELKKLNERKYVDYLRLKINDEKIFEYIKAFI
                     SSPVYTIICQADKVYKERVFFMPVNTSYISSITGQTYEKEHSILLQGVIDCLCIKDNH
                     AVIIDYKTDNVKQGNEKEHALMYLNQLEIYSKAVEEIMGIKVESKIIYFFKTNSHIDL
                     NLL"
     gene            complement(4044..7223)
                     /locus_tag="PLQ68_05465"
     CDS             complement(4044..7223)
                     /locus_tag="PLQ68_05465"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HXK72132.1"
                     /translation="MSIKVFYGLNKKKLTDEFIEQINDANQNSKASRLIVVIPEQFSS
                     YYEERLAMDKGFMRVEVMTFSRLTQRLFDIEYIQRSNSINEIGKTMLLYSLLNEDYIK
                     LDYYNRSRGFPGFSVLMKKTIEDLKNNDISYLDLMKLIEADNASDISRRKFFDLSKIF
                     EGYLNRLSDKGYSDQSDAFSFLCEHIKNKSSFDNCIIWFDRFDSFTSNEFDVIRQLML
                     KAERINISLCMDKNYAEDAAFDKSIFKRPTLTYNKIKEESQNLNISFFENKVFDKKEH
                     SSKEIKFLFENMFNTNFKSSKVIPKNISIFKASDIYEEVEYTAAKIMDAVTSRNIRFK
                     DILLVTGDISLYEMIAKAVFSQYDIPFYVNTRKNISDNPLMQFIISILDIYIWDYSYQ
                     HMTKFFKSIYSNLTEKESFELENYILKWGISGKTVWHTVWDYENSSYDIKMNELRLSV
                     IQFLDLFFDSIKGGCTAETFANNLYQLTEKMKVSEKIEKLSLYMNNDEIEQSKQCYNS
                     FIDILDQLVIITENEKKTAEYFLNILKTAFLEETVGVTPMTKDCVFLTSSSRTDIVDY
                     DTVFILGANDGSFPMTVNYNNLLNDSDYDLLKENGYEISSDPKEKIDEQEYITYTLLQ
                     SPNKYLYLSYCINNTEGGYLFPALIITKIYDIFTNIHTLTKEDIPQSYYAFNLKTGFN
                     LLYSNKQSIHQYCSGTEFEKFFDSLEDASGLTNKEMSDLIFNQEITITATMLERYMKC
                     PYSFLMAYGLKLDERSEFEYFSKNLGTLKHIVLFDVMNKVCNYEKEYNYDEIYAICEE
                     SAESISRINNIYRRDSILEYVKKRAVLQTAETVKVSLDILKDEKLKPRYFEAEFNDGQ
                     QLPAVIIENENVKIKITGKIDRVDSTFINNDEYFRIIDYKSSDKDTYLYKIEEGHDIQ
                     LALYAYAYHKAKNANLTGMYYMTVKEDYVEISKTSDESYKDNERVLSGYSFEDKDQSL
                     FDIVNEKNIKKRKIVDKQIAEKIFSSLEKTIIRTGERIKEGNFEVYPLNDNREPACKY
                     CEYKNLCGFDSSKGRYRVISPRKDDEIEW"
     gene            complement(7224..8126)
                     /locus_tag="PLQ68_05470"
     CDS             complement(7224..8126)
                     /locus_tag="PLQ68_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011024783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HXK72133.1"
                     /translation="MKKFIDYSEEVKKAIGMNKPLVALESTIISHGMPYPKNVETALM
                     CEDVVRKNGAVPATIAIIKGRIKIGLTHEEIEYLANAKGILKMSRRDIPAVIAQNQDG
                     AATVAATMIIANLAGIKIFATGGIGGVHRNAQNTFDISADLQELGKTSVAVVCAGVKS
                     ILDIGLTLEYLETLGVPILGYKTEKFPAFYTDDSGYKVDYQVNGAVHAAQILRTKWEL
                     GLNGGVVIANPIPSEYSMDKEYIDSVIKKAISQAELKGIKGKEITPFLLESIKDITKG
                     ESLSANIELVLNNAKVAAMIACEL"
     gene            complement(8127..9041)
                     /locus_tag="PLQ68_05475"
     CDS             complement(8127..9041)
                     /locus_tag="PLQ68_05475"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HXK72134.1"
                     /translation="MENGYIVVIGGINADITGKAVKDVIKNESNIGKISITAGGVARN
                     ICENLTRLGADCEFITTYANDYFGDMLQKSCQELNISLVHSYISNDYATGAYVSLEDH
                     IGEMEAAVCDSEGILNLPISHVEKLKVFIKHSQCIVLDCNLSEEMLEYVIKKYHEKKI
                     LIEAVSSAKVIKLRKNLNNVYAIKLNRNEFESLYEEPYTTENAQKMSKFYNNKIFVTE
                     GKNGSTGYEKGNAIHINAIPSKSIESVNGAGDAYAAGLAYGFMNGYDIRYSMNIANIL
                     SYITLSSKSAVSENISKYKVLTMLKEIN"
     gene            9165..10172
                     /locus_tag="PLQ68_05480"
     CDS             9165..10172
                     /locus_tag="PLQ68_05480"
                     /inference="COORDINATES: protein motif:HMM:NF014841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HXK72135.1"
                     /translation="METILLSNTPEKIYSVYNENILKEFALYTTIDTSKCYCKDYITI
                     NPFEFKDTKYILSTWGMPFFSKEEISLYFPSLEHIFYAAGSVQNFARPFMDCSVFVHS
                     AYMANAVPVAEYTVSQIILANKCFFQNTYYQSNGMLNEAISIKKHIKGTYGCNIGIIG
                     AGAIGTKVINMLNESYNVNIFVYDPFLNDNKAELLNVKKVNLHDIFTNCNVISNHLAN
                     NDQTKQMIDYDCLSLMGSYTTFINTGRGAQVVENDLIRALTEDSSRCAVLDVTDPEPP
                     VSESKLYTMPNVYLTSHIAGSMGDELYRMSKYMLEEFISTKTDQNFKHLITYSMLKTM
                     A"
     gene            10257..10332
                     /locus_tag="PLQ68_05485"
     tRNA            10257..10332
                     /locus_tag="PLQ68_05485"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10290..10292,aa:Thr,seq:cgt)
     gene            complement(10403..10696)
                     /gene="yhbY"
                     /locus_tag="PLQ68_05490"
     CDS             complement(10403..10696)
                     /gene="yhbY"
                     /locus_tag="PLQ68_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003483919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HXK72136.1"
                     /translation="MITTKQRSYLRKLAQKIEPIIQLGKEGINKNTLTQYEEALEARE
                     LVKTSVLKSCEYDAQEAAHDIADAIDAEVVQVIGNRFVLYKQSKEKKKINLDI"
     gene            complement(10706..11989)
                     /gene="obgE"
                     /locus_tag="PLQ68_05495"
     CDS             complement(10706..11989)
                     /gene="obgE"
                     /locus_tag="PLQ68_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010964572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HXK72137.1"
                     /translation="MFIDEAKIYVKAGKGGNGASTFRREKYEPSGGPDGGDGGKGGDI
                     IFVADNNIRTLIDVKYNRKYIAQDGAKGMKQRMTGKSGVDTIIRVPVGTLIWDDDKNK
                     LISDLNKDQMSKIICKGGRGGRGNTHFATPTRQTPEFSEAGEEGEEKNLFLELKLLAD
                     VGIIGMPSVGKSTLISVISAAKPKIADYHFTTLVPNLGMVRISDSTEFVVADIPGLIE
                     GASEGAGLGHEFLKHIERTKLLVHIVDASESEGRDAISDFEIINRELKKYNPDLYERY
                     QICALNKIDLCNTEEKRIRVNEIEEEIVKMGYEVLKISAATTKGTDELVKRCAVLLSE
                     LPNTVISTTAEDYAIYTQEDDELFTVRKEGNKYFVEGNWITKLVNSTNISNSESLRYF
                     QRLIREKKVIEELEKIGIQEDDIVVMDEFEFEYIK"
     gene            complement(12031..12285)
                     /gene="rpmA"
                     /locus_tag="PLQ68_05500"
     CDS             complement(12031..12285)
                     /gene="rpmA"
                     /locus_tag="PLQ68_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006875311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HXK72138.1"
                     /translation="MAHKKGVGSTKNGRDSESKRLGVKRSDGQSVLAGTILVRQRGTK
                     IHPGENVGIGGDDTLFAKASGTVQFSRMGKDKKKVSIVTQ"
     gene            complement(12304..12615)
                     /gene="rplU"
                     /locus_tag="PLQ68_05505"
     CDS             complement(12304..12615)
                     /gene="rplU"
                     /locus_tag="PLQ68_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007490927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HXK72139.1"
                     /translation="MYAIIETGGKQYKVSEGDTLFIEKIEAEDGTKVEFDKVLAVSTD
                     DGLKVGKPYVEKVKVTAEVEKNGKGKKIIVFKYKAKKGYKKMQGHRQPYTKVKIEKIS
                     L"
     gene            complement(12748..13836)
                     /locus_tag="PLQ68_05510"
     CDS             complement(12748..13836)
                     /locus_tag="PLQ68_05510"
                     /inference="COORDINATES: protein motif:HMM:NF024748.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A beta-lactamase-related serine hydrolase"
                     /protein_id="HXK72140.1"
                     /translation="MVKQKRRKSENEKKAVMVIIIIIITLGIPVLAIFIHKLNTKVSP
                     EISNSPIVTDTATQTESIEINPSIIIKPSQSAVPVTDTPTPTDVLPTVNLDVIEIRSL
                     NTLKTYLEYLLDTNEGKHGLSYYNIDTQEYMGIADTELYVAASSTKVPMVMYVYNAVE
                     KGILSFDDVVEYKQEDEESGTGIIIKGEFGDTYTIKQLAEYAITYSDNCAINMLIRVC
                     GEWNMVNYMNSLGAKVHYINQRWKTCPYDLTLYIKELISLIDSNPDHYNEIMENLIEN
                     TVAYYMSSKSILPDTIKMGVKMGINTTLPTLNETIVVFADQTYILSYCSTEIVIEESS
                     EILKQASLAIFEYVQNGYTIVKWDGTVR"
     gene            14028..>17014
                     /locus_tag="PLQ68_05515"
     CDS             14028..>17014
                     /locus_tag="PLQ68_05515"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HXK72141.1"
                     /translation="MNKYHKDVSIIGKSVLKPRCTSIPYSDLSNAKKDIRGLSPFFKS
                     LDGKWNFTFLENEFDIPEDFFLPEFDDQDWDIIPVPSSWQAQGYDTPHYINSRYPFPV
                     DPPHIPDMNPVGLYRTVFILPKAFENRNTVLHFGGVNSSFVLYVNGKEVGYSQGSHMP
                     SEFDITSFTRPGANLIAVKVYKMSATSYLECQDFYRHSGIFREVYIFSQHKSSITDFT
                     VRTTLCDLFVNVETTLLKDFTLIFNLFDNNDTLVFTKTTSNNTINLHIEGILPWTAET
                     PNLYTLIIQLKDSKGIITDIRSTRVGFRTVSINDGVFMINNTPIKIKGTNRHDTYALT
                     GHAVSNEVMKNDVIMMKRHNINAIRTSHYPNDPFLYKLCDEYGIYVIDEADIETHGFY
                     YDDPEYDLSDKPEWEPHFISRAERMVMRDKNHPCIIMWSLGNETRFGKNHLSMIKYIR
                     SVDDRPIHFERAISDESVDVVSQMYTNHRNLDIEGSKIDKRPFFLCEYAHGMGCGPGG
                     LKDYWDIIYKYPRLIGGCVWEWCDHGLLSYTDDMVPFISYGGDFDDFPNDSNFCLDGY
                     TTADRIPKACLIELKKCYEPVTVIDYDKHTNTVKIKNMQDFADASNIAVYYELSKDGD
                     VIYTGNISSLNILAQETKSYTLDFDANIFGDIQLTFFFEYNESTDFAEKGFEITRCQI
                     ILDEKFEYMKPYIGNDLVSVETKTDYIVLGDDFKISFSKIYGNLYSYYFKGNDLILGK
                     GLCENFYRAPTDNDVKIAKKWKEYGLDNLQKRIDKVQFICNKKEAIFTVTSVFGSSTK
                     KPVLKTITNYTVYTNGQVTVKSTYEPLNDFIKLISSDEENNNFYLPRFGITCQISDEY
                     EYVKWFGRGFHQSYEDMKSSAVVGIYEGLVEELSENYEKPQENGNRMDSRWFSLKNTF
                     GQGIFFGGLNTFNFSTSYYNADQLEKSRHLYQLKREGLININIDFKQSGLGSASCGPI
                     PEDKYILKVNSNSFSFTFSPFQDS"
     assembly_gap    17015..17114
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<17115..18253)
                     /locus_tag="PLQ68_05520"
     CDS             complement(<17115..18253)
                     /locus_tag="PLQ68_05520"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006352856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HXK72142.1"
                     /translation="MYNTEKAYKQAKEYYKRYGIDTEKAIKICDSIPISIHCWQGDDV
                     GGFEKSSKDLTGGIQVTGNYLGKARNAEELRNDLDFAMKYIPGVKKVNLHANYQEAED
                     FVDRDEIKPEHFKKWADWAVDRKIGLDFNPTYFSHPKSDNGTLSAVDDKIREFWINHG
                     IACRRIGQYFYERTGKTCVINHWTSDGSKEVPHDTLAPRLRLKDSYDRIFKETADVKG
                     VVDGLESKLFGIGLESYTTGSYEFCMGYVMNKKKNHIILTLDTGHYHPTEVVSAKLSG
                     IFTFQKSLLLHVSRPVRWDSDHVVSFDDETKAIFEELVKLNKLKSTYVATDYFDASIN
                     RVMAWVTGLRNTRKAILAALLQPNHMLTDAENSNDLSSRLAIKEE"
     gene            complement(18279..19313)
                     /locus_tag="PLQ68_05525"
     CDS             complement(18279..19313)
                     /locus_tag="PLQ68_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009269759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2804 domain-containing protein"
                     /protein_id="HXK72143.1"
                     /translation="MMQHEITEKKRLLNEDGVLTEPGYAKKFLFDYLRSDIKANSLRI
                     KEWDYYLINNDKFALALTIADNSYMGLDSVSFIDFNNKWQHTKSYMSFMTKGIKSLPS
                     SSLNGDTISSGKSYTIKFINDVKKRILSAHLDDFMKNIQFDAEIELTDEPKESMVIAT
                     PYKEDKLAFYYNQKINCMKAEGFVKFNGNIYEFKKNDSFAVLDWGRGVWTYKNTWYWG
                     SASGYIDNIPFGFNIGYGFGDTSAASENMIIYNGKAHKLDRVKFVIPKKDGKDDFLSP
                     WKFISNDNRFTMSFEPVLDRSSCTSLGIIKSDQHQVFGRFTGEVVLDDNKIIRVKDLL
                     GFAEKVFNKW"
     gene            complement(19449..20660)
                     /locus_tag="PLQ68_05530"
     CDS             complement(19449..20660)
                     /locus_tag="PLQ68_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004797874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HXK72144.1"
                     /translation="MNILLSLCLAMLAGLLMTRLVKIFGLPNVTGYIIAGLLIGPFCF
                     KFIPTELLEGFSIITTVALGFIAFSIGGEFKIEHLKKLGKKVVVITVVQALMTVLVVD
                     LLLFIASFFIPGLDAPIILTLGAIATATAPAATLMVVRQYKAKGIVTQTLLPVVAGDD
                     AIGLIIFSISLSIAKALANHEALTFKTSVLIPLYEISMSLIIGAVLGFILSLSMKFFK
                     SRANRISLIVAITFGGVALSQLLELSALLTCMMIGAMYANICKNNDPVMEAYDRWTPP
                     VFLLFFVISGAELNVKMIISVGVIGILYLVARSIGKYFGAFAGSKMVHAHPNVTKYLG
                     LALLPQAGVAVGMAQIVMMQLPQYGDSIRTVILCATLIYELIGPVVTKIALTKAGEIE
                     PKLKVSKKGNK"
     gene            complement(20665..21009)
                     /locus_tag="PLQ68_05535"
     CDS             complement(20665..21009)
                     /locus_tag="PLQ68_05535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72145.1"
                     /translation="MQLLVIILNKTEFLSKLLNEMMENNICGATVLESTGMIQVISEQ
                     SIEPPSIFGSLREFINPSRESSKTVFMVLPDEQIEIAKRIVNQVTGGLEKPNTGIMFT
                     IPISFAEGITMV"
     gene            complement(21087..22391)
                     /locus_tag="PLQ68_05540"
     CDS             complement(21087..22391)
                     /locus_tag="PLQ68_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HXK72146.1"
                     /translation="MNTMIKNADILTMDDEFTILNNQVICIQDNKIVYIGEDEPFFFL
                     PNRIINAKGKIVMPGLINSHTHCAMTLMRNRSNDLPLERWLSEGIFPVEANLTYNDVY
                     SGAMLGITEMIKNGITSYLDMYYIHEASLEAISQTGIRANLSYGVSTSSKVKELGKQG
                     AEEYCKNFVINNKKAFKGRVNTSIEVHSVYLVDEPELVLSAQMAKETDTLIHIHLHET
                     ETEVENCVKKYGLTPIQVCEKTGILSNKVTAAHTVHVNDDDMALLRDRKVIPVHNPSS
                     NMLLGSGFADISKMIEMGITPALGTDGAASNNTLDMFKEMHLAALIHKGYKHNSSAIG
                     AKQILKMATTNGSKALGFDDCGIIKKDMLADLIIVNTDSINMTPYHDPVSALVYSARA
                     TDVDTVLVNGEILMQNRELTSIDEQKVKFEATRASKRLYGEK"
BASE COUNT         6944 a         3976 c         3019 g         8425 t
ORIGIN      
        1 caaatgcctg attctagaag tcgtttaagt ttcgaaacac ctgtaatcct gttgtttatg
       61 tcaacattat tcttttttaa aatatttatt gcttttttat attgtttttt ttcaatttcc
      121 cattcgagtt cataatcgat attactatta tacttattca aatctatttg catatctggc
      181 atttttaaat caagtgtcac atgacctctt atagtttcta aagttccttt gtattcagcc
      241 ttaattttat tatcgttctt caatcccaaa tagtcatata aacagttagg gttattgtta
      301 ataataatat aatctgtttc tgatatatcc attgaatact ctgttttgtc tttgagtgct
      361 tttaactctt tagtattatt actttcttta tactgaataa agaatttatc attaattttt
      421 cttattctta cacataaatt atcatttagc ttatagtagt ggttagtttg cgaatatata
      481 ttatgtgctt tggagaagaa agtgttatat tcgtccttag tgatcattac tttcaattct
      541 gtctctgtgt tttttctaat atttatcatt ttcctcttcc taaaaactta gttcttatta
      601 ttataacaga ttaaggtcaa tatgcgaatt agtcttaaaa aagtatataa ttttactttc
      661 aaccttaatt cccattattt cttcaactgc tttgctatat atttccaact gatttaaata
      721 catcaatgca tgttcttttt cattgccttg ttttacatta tcagtcttat aatcaataat
      781 aaccgcatga ttatctttta tacatagaca atctataaca ccttggagaa gaatagaatg
      841 ctccttttca taagtttgtc ctgtaattga agatatataa cttgtattta caggcatgaa
      901 aaaaactctt tctttgtata ctttatcagc ttgacatatt attgtgtaaa ctggacttga
      961 aataaatgct ttaatgtact caaatatctt ctcatcattt atctttaacc ttaaataatc
     1021 aacatactta cgttcattca attttttaag ttcaatagat gccataatct tatgcatcat
     1081 tgtgcctttt tctgcagcat tttgtttact gcctgattta tttagaaatt caggtctttt
     1141 tttaacagtt gttttatatt gattataaaa ttcttctata tcataattct tgctcttata
     1201 tttagctagt tccgttgcag agattttcct attaagagtt gtttcttcaa tgtaaggata
     1261 ctcatatccc aaacgagcca ttatatcttg aaagtatgct gttttatcaa aattttcttc
     1321 tttctttgtc tgctgtttgc tttctgatat ataagaagat atcactgcac taaattcaga
     1381 tgcttttata atattctgta ttgaattatc agaatttggt aatatccagt caaaataatt
     1441 ttttgcatca tcagctatta gtacatcttt tttatctggt tctttaccta ccattatcaa
     1501 tttttctttt gctcttgtaa gtgcaacata aagtattctc atttcttctg ataaattttg
     1561 tcttttaata cttaaagata aagcattttt agttatatgc ttgtgatatg ttttgttaat
     1621 gtcactaata tattcaattc caaaacctaa atccctgtcg ataagcaatc tgttttttgt
     1681 ttcttcaatg ttaaatttcc ttgaacagcc tgctagtatt acaactggaa actctaatcc
     1741 tttgctttta tgaatactca ttacattaac tgaatctaaa gattgtgttt ttaaaatttc
     1801 cgatataata tctttatctt tctttttttt acttttaata aaattaagaa aaccgcttac
     1861 atcattaatt cctttcttat gacattcaac cgctagcgca tataatcttt taagaagttc
     1921 ctggttttca gatacataat agaatcctga ttttgtcata gcataaaata gaattttttc
     1981 aagtgtattt acttttgcga aatttcttat agctgttagg tcatctatta acctttttat
     2041 cttgcattca agtaattcat tgttttctag cagcttattc tctttgtaat ttattaaatc
     2101 attatacaaa ccatacctgc ttttttttat tttagtaatt tctttctcat tgaatgcata
     2161 aactttattt ttcataacct gagcaagata aatatcctga taaggattgt cagtcacatt
     2221 caacaaagca tagacatcag atatgctctt atcgttcatg tactctacac tttggtcaga
     2281 atatgctaaa ataccacact tatttagtat ttcacagtat ttttttgcat atgggttaac
     2341 tgatcgtaac agtattacta tatcagaata tcttattgct ctagtatcct ttttattact
     2401 gtcaaatatg gttataccac tatttatcaa ttgttttatc tcttggacaa taacaaatgc
     2461 ttctacttcg atttcagtca ttgtagggaa ttcattatac aaatctttac tatactcata
     2521 cttcaaaatt ttcggtaaat aaatatcctc tgaagaatcc ttatcaaagt tataattacc
     2581 atatctaagc ttttcttctt ctgaatactc cacttcccca agattgtcat tcattatatt
     2641 cgagaatatt gaattaacat attccaatat tgtgtttctg cttctgaaat tataactcaa
     2701 attcagcttc atgccttttt cagtggattt ttgaaactgg gttgctttat ttctaaaaat
     2761 agccgtttga gcatttctga atctatatat gctttgcttt aaatcaccaa cagcgaatac
     2821 attaccttct cttcttattt gatttattat ctcttcttga atatagttaa agtcttgata
     2881 ttcatcaatt aaaatatgct taaatttatc cctgtatagt tcataagctc ctgattttaa
     2941 gcatttcaga gccatatgct ccatatctga atagtcaatt acttttgctt tacttttctt
     3001 ttcagaatat gctttatcaa cacataagag cgtacctgtg aattctttta aaatcggata
     3061 catgatttca caatcttttt taatattttc agtatcatca taaatttgag aggttatgtc
     3121 aattattgag ttcttaaatg cttttctata ttcttttatt gcatccacat attcctcatt
     3181 gaaatctttc ttttttctgg gtaaagatat gtattttaaa tcataaaaat attcttttaa
     3241 agcatcccaa tcaagtgttt ctataaaatc atatatattt tttagtatgt taaaatcatg
     3301 attaagcata tcctcatagt gacaatactc tacagtacca gatagtatac tacttatatt
     3361 gtttatatat atttcacacg aatttagttt tcttaatata tgttttttaa tttcaatccc
     3421 atacttggaa ttaaatatca attcatgttt aataactgac tcttcagttt tttttgtaag
     3481 ccattgttca taatctgggt aactttttac tgttttcagt atcgatatga ttaaagatgt
     3541 cagagtatca agatttttcc catatcccaa tgttgataaa agcttcagaa aattttcctc
     3601 tttattctgt aatttatttt tataaacact agtaattgca tcttctaaat aattatctgc
     3661 aatagtttca tcaactacgg aaaagttggg atctatattg ataagattag cgttatttct
     3721 gattacttca ctacaaaaag aatgtaatgt cgatatcgtt gctcttgaaa tattgcgata
     3781 ctgctttttc agatattcat tttctggaat ttgaaggatt ttttctttta gagcttttct
     3841 gactctttct ttaatttcac ctgctgcagc attagtaaat gtagttacaa gaaggttttc
     3901 gcattcagta cccaattcat catctgacaa tatactgata atcctttcaa taagagttgc
     3961 agttttccca ctgcctgctg ctgctgaaac taatatatct gtatttctta atgaaatagc
     4021 ttgttgctgc tgttccgtcc attttaccat tctatttcat catcctttct tggagaaata
     4081 actctatatc taccttttga actatcaaaa ccacataagt tcttatactc acagtattta
     4141 caagctggtt ccctgttatc attaagagga taaacttcaa aatttccttc ttttattctt
     4201 tcacctgttc ttataattgt tttttccaga cttgaaaata tcttctcagc gatttgttta
     4261 tcaactattt tcctcttctt aatatttttt tcattaacaa tatcaaaaag cgattgatct
     4321 ttatcttcaa atgaatatcc gcttaatacc ctttcattat ctttatatga ttcatctgat
     4381 gtttttgaaa tttctacata atcctcttta acagtcatat agtacattcc tgtcaggtta
     4441 gcatttttag ctttatgata tgcgtaagca tacaaagcaa gctgtatgtc atgtccttct
     4501 tcaattttat ataaataagt atccttatct gaagatttat aatcaattat tctgaaatat
     4561 tcatcattat taataaaggt tgaatcaact ctgtcaatct ttccagttat tttaattttc
     4621 acattttcat tttcaattat cacagcagga agctgctgtc catcattaaa ttctgcttca
     4681 aaatacctag gtttcaattt ttcatctttt aaaatatcaa gtgaaacctt aactgtttct
     4741 gctgtctgca gaacagctct tttcttcaca tactctaaaa tcgaatctct tctgtagata
     4801 ttatttattc tgctaatact ttctgcagat tcttcacata ttgcataaat ttcatcgtaa
     4861 ttatactctt tctcataatt gcatacttta ttcattacat caaataaaac tatatgcttt
     4921 aatgttccaa gattttttga aaaatattcg aattctgacc tttcatcaag tttcaatcca
     4981 tatgccatta agaaactata tggacatttc atatatcttt cgagcatagt tgcagtgata
     5041 gttatctcct ggttaaatat taaatcagac atttctttgt tagttagtcc acttgcatct
     5101 tctaaagaat caaagaactt ctcaaattcc gtaccagaac aatattgatg aatcgactgc
     5161 ttatttgaat acaagaggtt aaatccagtt ttaagattaa aagcatagta actttgtggt
     5221 atatcttcct tagttagtgt atgtatgtta gtaaaaatat cataaatctt tgtaataatt
     5281 aaagctggaa aaagatatcc accttcagta ttatttatac aataacttaa ataaagatat
     5341 ttatttggac tttgcagtaa agtgtatgta atgtattcct gttcatcaat cttttctttt
     5401 gggtcagaac ttatctcata cccgttttct ttaagtagat cataatcaga atcatttaat
     5461 agattattat aattaacagt cattggaaaa ctgccatcat tggcacctaa aatgaaaaca
     5521 gtatcataat ccactatatc tgttctggat gaactagtaa gaaacacaca gtcctttgtc
     5581 atcggtgtta cacctaccgt ctcttctaaa aatgctgttt ttaatatatt aagaaaatac
     5641 tcagctgtct ttttttcgtt ctcggtgata atcacaagtt ggtctagaat atctataaat
     5701 gaattgtagc actgtttcga ctgttcaatt tcgtcattat tcatgtataa gctgagtttc
     5761 tcaattttct cacttacttt cattttttct gtaagttgat aaagattatt agcaaatgtt
     5821 tcagcagtac atcctccttt tatgctatca aaaaaaagat caaggaattg tataactgac
     5881 aacctaagct cattcatttt gatatcatat gaactatttt cataatccca aactgtatgc
     5941 catacagttt ttcctgatat cccccatttc aaaatatagt tctctaactc aaaagactct
     6001 ttttctgtaa gatttgaata aatgcttttg aaaaattttg tcatatgctg ataactataa
     6061 tcccaaatat aaatatctag aatagatata atgaactgca taagaggatt atcagatata
     6121 ttctttcttg tattaacata aaaaggaata tcatattgac tgaataccgc tttagcaatc
     6181 atctcatata aactaatatc acctgttaca agcagtatat ctttgaacct tatattccta
     6241 ctagtaacag catccattat tttagcggca gtatattcaa cttcctcata aatatcgcta
     6301 gctttgaata ttgaaatatt ttttggtatt acttttgaag atttaaaatt tgtattgaac
     6361 atattttcaa aaagaaattt tatttcttta gaactatgtt cctttttatc aaatacttta
     6421 ttctcaaaaa aacttatatt taggttttgt gattcttcct ttattttgtt gtatgtcaat
     6481 gtaggtcttt taaatatact tttgtcaaaa gcagcatcct cagcataatt tttatccatg
     6541 cataatgaaa tatttattct ttcagcttta agcatcaact gtctgattac atcaaattca
     6601 tttgatgtaa atgagtcaaa tctatcaaac catataatac agttatcaaa actggattta
     6661 tttttaatat gttcacacaa gaagctgaat gcatcagatt gatctgaata gcctttatca
     6721 gaaagtctat ttaaatatcc ttcaaatatt tttgaaagat caaaaaactt tcttctagaa
     6781 atatctgaag cattatcagc ttcaattaac ttcattaagt ccaaatatga aatatcatta
     6841 ttctttaaat cttctattgt ttttttcatt aaaactgaaa agcctgggaa cccccttgac
     6901 ctgttataat aatccagttt aatatagtct tcatttaata atgaataaag aagcattgtc
     6961 tttccaattt catttattga gttgcttctt tgaatatatt ctatatcaaa aagcctttga
     7021 gtaaggcggc tgaatgtcat aacttcaaca cgcataaacc ctttatccat agcaaggcgt
     7081 tcttcataat acgatgagaa ctgctctgga ataacaacaa taagtcttga tgccttactg
     7141 ttttgatttg catcatttat ctgttctata aattcatctg taagtttttt cttatttaat
     7201 ccataaaaga ctttaatact catttacagc tcacatgcta tcatagcagc gacttttgca
     7261 ttattaagga caagttcgat atttgcagaa agactctctc cttttgttat atcttttatg
     7321 ctttcaagaa gaaatggagt tatttctttg cctttaatac cttttagttc tgcttgtgaa
     7381 atagcctttt ttatcacact atcaatatat tccttatcca ttgagtattc agaaggtata
     7441 ggattagcta ttacaacacc accgttaaga cctaattccc attttgttct tagtatctga
     7501 gcagcatgaa ctgctccatt tacttgataa tctactttat aaccgctgtc atctgtataa
     7561 aatgctggaa atttctctgt tttatagccc aaaataggaa caccgagtgt ttctaaatac
     7621 tccaatgtca aacctatatc aagtatactt ttaactcctg cacaaacaac tgctactgat
     7681 gtcttaccca gctcctgaag gtcagctgat atatcgaatg tattttgggc atttctgtgt
     7741 acaccgccaa ttccgcctgt ggcaaaaatc tttatacctg ccaaattagc aattatcata
     7801 gtagcagcaa cagttgctgc tccatcttga ttttgtgcta taacagcagg tatatctctt
     7861 cgtgacatct taagtatacc tttagcattt gccaggtact caatttcttc gtgagtaaga
     7921 ccgattttaa ttctgccttt tattattgct attgttgcag gtacagctcc atttttacga
     7981 actacatcct cacacatcaa ggcagtttca acatttttcg gataaggcat accatgacta
     8041 attattgtag attccaatgc cactagaggc ttgttcatac ctattgcctt tttcacttct
     8101 tctgaataat caataaattt tttcatctaa ttaatttcct ttaacatagt taatacttta
     8161 tattttgata tattttcact tactgcactt ttacttgata gggtgatata acttaatatg
     8221 tttgctatat tcattgaata tcttatatca taaccattca taaatccata tgccaaacca
     8281 gctgcatatg catcccctgc accattaaca gattctattg attttgatgg aattgcatta
     8341 atatgaatag catttccttt ctcataacct gtacttccat ttttaccctc agttacaaat
     8401 attttgttat tataaaattt agacattttc tgggcatttt ctgtagtata tggttcttca
     8461 taaaggcttt caaattcatt cctgttaagt tttattgcat aaacattatt taagtttttc
     8521 ctaagtttta taacttttgc tgaagataca gcttctataa gtattttctt ttcatgatac
     8581 ttctttataa catactctag catttcttca cttagattac agtcaagaac tatgcattgc
     8641 gaatgtttta taaacacctt caatttttca acatgactaa ttggtaagtt caaaatacct
     8701 tccgagtcac aaactgcagc ttccatctct ccgatatgat cttcaagtga aacatatgct
     8761 cctgttgcat aatcatttga tatataggaa tgaactaaag aaatatttaa ttcttggcat
     8821 gatttttgca acatatctcc aaaataatca tttgcataag ttgtgatgaa ttcgcagtca
     8881 gctccaagcc ttgtaagatt ttcgcatata tttcgtgcca ctccacctgc agttatgctt
     8941 atttttccta tatttgattc gtttttaata acatccttaa ctgctttgcc cgttatgtca
     9001 gcatttatgc ctccaataac gactatgtat ccgttttcca tatatatgtt cctctcaaca
     9061 ataatataat atcataatta acagttaaaa taaacgaaat ataagttaga aaatctgtat
     9121 caatattgtt tgacatacca tatttcaaat attagaataa gactatggaa acaatattat
     9181 taagtaacac accagaaaaa atttactcag tctataacga aaatattcta aaggaattcg
     9241 ctttatacac aacaattgat acttcaaaat gctattgcaa agattatatc actattaatc
     9301 catttgaatt caaagataca aagtatattt tatccacatg gggtatgcct tttttttcta
     9361 aagaggaaat atcgttatac tttccttctt tagaacacat tttttatgct gctggttctg
     9421 tgcagaattt tgcacgtcct tttatggatt gttctgtttt tgtacattct gcttatatgg
     9481 caaatgcagt acctgttgca gagtatactg tcagtcagat aattcttgca aataaatgtt
     9541 tttttcaaaa cacatattac cagtctaatg ggatgcttaa tgaagccata tcaataaaaa
     9601 aacacataaa aggcacatat ggatgcaata taggtataat aggagcagga gcaatcggga
     9661 ccaaagttat taatatgtta aatgaatcat ataatgtgaa catttttgta tatgatccat
     9721 tcctaaatga taataaagct gaattgctaa atgtaaaaaa ggttaacctt catgatattt
     9781 ttacaaattg taatgtaata tcaaatcatt tagcaaataa tgaccaaaca aaacaaatga
     9841 ttgactacga ttgtttaagt cttatgggaa gttatactac atttattaat actggaagag
     9901 gagctcaggt cgtggaaaat gatctaatca gagcattaac tgaagatagc tcaagatgcg
     9961 ctgtgcttga tgttactgat cctgaacctc ctgtttcaga aagcaaatta tacacaatgc
    10021 caaatgtata tttgacatct catattgcag gttctatggg agatgaatta tatcgcatgt
    10081 ccaaatatat gctagaggaa tttatttcta ctaaaactga tcagaatttc aagcatctta
    10141 ttacatattc aatgcttaaa acgatggcat gattatacat gttttgaata aacactttta
    10201 cttatttcaa ttgacattta catatctttg gtttatattt ttaagcacgg tctgatgctg
    10261 gtgtggctca gcaggtagag cagctgattc gtaattagca ggccaccggt tcaaatccgg
    10321 tcaccagctc caaaaaatcc tcatatatat tgaggatttt attttttgtg ttactaaatg
    10381 aaattaatat aaaactaaaa ttttatatat ctaaatttat cttcttcttt tcttttgatt
    10441 gtttgtataa aacaaatctg tttcctataa cctgaaccac ttcagcatca attgcatccg
    10501 caatatcatg tgcggcttct tgagcatcgt attcacagct tttaagaaca cttgtcttta
    10561 caagttcacg tgcctccaaa gcctcttcat actgtgttaa agtgttttta tttatacctt
    10621 ctttacccaa ctgaataatt ggttcaattt tttgggctaa ttttcttaaa tatgatcttt
    10681 gttttgttgt tatcattttt acctcttatt taatatattc aaattcaaat tcatccatta
    10741 ctacgatatc atcttcctgg attccaattt tctcaagttc ctctattact tttttctcac
    10801 gaataagccg ctgaaaataa cgtaatgact cactattact tatattcgta gagttaacaa
    10861 gttttgtaat ccaattccct tctacaaaat atttgttacc ttcttttctt acagtgaaca
    10921 attcatcatc ttcttgggta tatattgcat aatcttcagc tgtagttgag ataacagtat
    10981 ttggtaattc agaaagtaat actgcacatc ttttaactaa ttcatcagtt ccttttgttg
    11041 tcgctgctga aatttttagt acttcataac ccatctttac tatttcttct tctatttcat
    11101 taactctgat tcttttttct tctgtgttac ataaatctat cttatttaat gcacatatct
    11161 gatatctttc atatagatca ggattatatt ttttcaattc cctgttaata atttcaaaat
    11221 cactaattgc atctctgcct tctgattcag aagcatctac tatatgtact agtaatttag
    11281 ttctttcaat gtgttttaaa aattcatgtc ctagaccagc accttctgaa gccccttcaa
    11341 tgagtccggg gatatctgct actacaaatt cagtactatc agaaatcctt accattccta
    11401 agtttggtac aagtgttgta aagtggtaat cagcgatttt tggttttgca gctgaaataa
    11461 ctgatataag tgttgatttt cctacagatg gcattccaat aatgccaaca tccgctaata
    11521 atttaagttc aagaaataaa tttttctctt ctccttcttc tccagcttcg gaaaactccg
    11581 gtgtttgtcg tgtaggggtt gcaaaatggg tattacctct gccacctctg cctcctttgc
    11641 atattatctt tgacatctgg tctttattta agtcgcttat aagtttattt ttatcgtcat
    11701 cccatataag tgtaccaaca ggaactctta ttatggtatc aacaccagac ttgcctgtca
    11761 tacgttgttt catacctttt gctccatctt gagcaatata tttacggtta tacttaacat
    11821 caataagcgt acgtatgtta ttatcggcga caaaaatgat gtccccgcct ttaccgccat
    11881 cgcctccgtc aggtccgcca gatggctcgt atttttctct tctaaatgta ctggctccgt
    11941 ttccgccttt gccagctttt acatatattt ttgcttcatc tatgaacata attaaaaaaa
    12001 accgcttctc ttgggaagcg ggtgtttttt ttactgggtt acgatgctta cttttttctt
    12061 gtcttttccc atacgagaga attgaaccgt tccacttgct ttcgcaaata aagtatcgtc
    12121 tccgccgatt ccgacgtttt cacctgggtg aatcttggtt cctctttgtc ttacaagaat
    12181 ggttcctgca agtactgatt gaccatcgct tcttttaacg cctaatcttt tgctttcact
    12241 gtctcttccg ttttttgtac ttcctacgcc cttcttatgt gccattaaaa ctcacctcca
    12301 atgctataac gatatctttt caattttaac ttttgtataa ggctgtctgt gaccttgcat
    12361 tttcttgtat cctttttttg ctttatactt gaaaacgatt atctttttgc ctttaccgtt
    12421 cttttctact tctgctgtta cttttacttt ttcaacataa ggttttccaa cctttagtcc
    12481 gtcatcagta gatactgcaa gaactttgtc aaactcaact tttgttccat cttcagcttc
    12541 tatcttttcg ataaagagcg tgtcaccttc agatactttg tactgttttc ctccggtttc
    12601 aataattgca tacattatta tttgcctcct gctatccagt ctcgccttgc aaggtagtat
    12661 atataatacg tttttgacct gccaagagcg gctacctgtg gtattctacc acatgcaaat
    12721 atcagtgtca acaaaataaa tttctgccta tctgacagtt ccgtcccact taacaatggt
    12781 atatccattc tgtacatact caaatattgc aagacttgct tgtttcaaaa tttcagatga
    12841 ttcttcaatt acaatttcag tagaacaata tgataatata tatgtctggt ctgcaaatac
    12901 tacaattgtt tcattaagtg taggtaaagt agtatttata cccattttta cacccatttt
    12961 tattgtatca ggaagtatgg attttgatga catataatat gcaacagtat tttcaattaa
    13021 attttccatt atttcattat aatggtccgg attactgtcg ataagactga ttaattcttt
    13081 tatgtaaaga gtaaggtcat atggacaggt tttccaacgt tgatttatat agtgaacttt
    13141 ggcacctagt gaattcatat agttgaccat attccattca ccgcaaactc ttattaacat
    13201 atttatagca cagttatcac tatatgttat agcatattca gctagttgtt ttattgtata
    13261 agtgtctcca aactcacctt ttattattat ccctgtacca gattcttcat cttcctgttt
    13321 atactcaact acatcatcaa aggacaatat ccctttttca actgcattat atacatacat
    13381 taccattggt acttttgtag aggaagcagc aacatacagt tcagtatctg ctatacccat
    13441 gtattcttgt gtatcaatat tgtagtaact taaaccatgt ttaccttcat tggtgtctaa
    13501 aagatattca aggtatgtct ttaaagtatt cagagatctt atctctataa catcaagatt
    13561 aactgttggt aaaacatccg taggtgtagg agtatctgta acaggtacag ctgactgaga
    13621 aggtttgatt ataattgaag gattaatttc aatactttct gtttgtgttg cagtatcagt
    13681 cacaatcggt gagttgctaa tttctggaga taccttagta tttaacttgt gtataaaaat
    13741 agcaagaaca ggaattccta aagtaattat tataataatt attaccatca ctgctttttt
    13801 ttcgttttct gactttcttc ttttttgttt aaccatataa attaccttta gaagattata
    13861 tcataagcac tatcaaatat tttatacatt tttgcttata atatttttac atcatcacaa
    13921 tattttcaca taaaaagtgt attatctttt agtaacaata tattatagca gtataatgtt
    13981 tgaggcaacc tttcgcatgt acgcataatt gttttaggag gtttttgatg aataaatatc
    14041 ataaagatgt tagtatcatt ggtaaatcag tattaaaacc cagatgtaca tcaattcctt
    14101 attctgattt gtctaatgca aagaaggata taagaggatt atcacctttc tttaaatctc
    14161 tcgatgggaa atggaatttc acgtttttag aaaatgagtt tgacattcct gaagatttct
    14221 tccttcctga gtttgatgat caagattggg acatcattcc tgtaccatca tcttggcagg
    14281 cgcaagggta tgatactccg cattatatca attcaagata tcccttccct gtggatcctc
    14341 cacatattcc ggatatgaat cctgttggat tatacagaac agtatttata ctaccaaaag
    14401 catttgaaaa ccgtaatact gttttgcatt ttggtggagt aaattcctct tttgttctat
    14461 atgttaacgg aaaagaagtg ggatattctc aaggttcaca tatgccttct gagtttgata
    14521 ttacatcttt tacacgccct ggagctaatc ttatagcagt taaagtctac aaaatgagtg
    14581 caacaagtta tttggaatgc caggattttt atagacactc agggatattc agagaagttt
    14641 acattttttc ccaacacaaa tcttctatca ccgatttcac agtcagaacc actctttgtg
    14701 atctttttgt caatgtcgaa acaacactat tgaaagattt tactttaatt ttcaatttat
    14761 ttgataataa tgatacactt gtattcacaa aaactacttc aaataatacc attaatcttc
    14821 atattgaagg aatattacca tggactgctg aaactccaaa tctatatacg ctaattattc
    14881 aacttaaaga ctctaagggt ataatcactg atattcgaag tactagagtt ggattcagga
    14941 ctgtttctat aaatgatgga gtctttatga ttaataatac ccctattaag attaaaggaa
    15001 cgaacagaca tgacacatac gcattaactg gtcatgctgt atcaaatgaa gttatgaaga
    15061 atgatgtaat tatgatgaaa cgtcataaca ttaatgctat aagaacttct cattatccta
    15121 atgatccttt tttatacaaa ctttgtgatg agtatggaat atatgtaata gatgaagcag
    15181 atatagaaac acatggattt tattacgatg acccagaata tgatttatca gataaacctg
    15241 aatgggaacc ccacttcata agtagagccg aaagaatggt aatgcgtgat aagaaccacc
    15301 cttgtatcat aatgtggtct ttaggtaatg aaacaagatt tgggaaaaac catctatcga
    15361 tgattaaata tattagaagc gttgatgaca gacctataca cttcgaaaga gccatatcag
    15421 atgagtctgt tgatgttgta tcacagatgt acactaatca tagaaacctt gatattgagg
    15481 gctctaaaat tgataagaga cctttcttcc tttgcgagta tgcacacggt atgggatgcg
    15541 gcccaggagg attaaaagat tattgggaca ttatatataa gtatccaaga ctaataggag
    15601 gatgcgtatg ggaatggtgc gatcatggtt tactttcata tactgatgat atggttccat
    15661 ttatatctta tggtggtgat ttcgatgatt ttccaaacga ttccaacttc tgccttgacg
    15721 gatacacaac tgcagataga ataccaaaag catgccttat tgaactaaag aagtgttacg
    15781 aaccagtaac agttatagat tatgacaagc atacaaatac agtaaaaatt aagaacatgc
    15841 aagattttgc agatgcttcc aatatcgcag tttattacga attatcaaaa gatggtgatg
    15901 taatttacac gggtaatatt tcaagcttaa atatacttgc acaagaaaca aaatcatata
    15961 ctcttgattt tgatgcaaat atttttggtg atatacagct tacattcttc ttcgaataca
    16021 atgaaagcac agattttgct gaaaaaggtt ttgaaattac cagatgccag attattcttg
    16081 atgagaaatt cgaatacatg aaaccttaca ttggaaatga tcttgtctca gtcgaaacca
    16141 aaacagatta catcgttcta ggtgatgact tcaaaatttc tttttctaaa atatatggca
    16201 acttatattc ctactacttt aaaggaaatg atctgatttt aggtaaaggc ttatgtgaaa
    16261 acttctatcg tgcaccaacc gataatgatg tgaaaatcgc aaagaaatgg aaagaatatg
    16321 gacttgataa tctacaaaaa agaattgata aagtccagtt catatgcaat aaaaaggaag
    16381 ctatatttac tgtaacatca gtatttggtt catcaacaaa aaaaccagta ttaaaaacaa
    16441 ttacgaatta tactgtttat acaaatggac aagtaactgt gaaatctact tatgaaccac
    16501 ttaatgactt tatcaaatta attagttctg atgaggaaaa caacaatttt tatctgccac
    16561 ggttcggtat aacatgtcaa atttctgatg aatatgaata tgtaaaatgg tttggcagag
    16621 gttttcatca aagttacgaa gatatgaaaa gcagcgctgt tgtcggtata tatgaaggtt
    16681 tagttgaaga gttaagtgaa aattacgaaa agcctcagga aaatggtaat agaatggatt
    16741 ccagatggtt tagtttgaaa aatacatttg gccaaggtat attttttggt ggactgaaca
    16801 cattcaattt cagtacctct tattataatg ctgatcagct agaaaaaagt cgtcatttat
    16861 atcaattaaa aagagaaggt ttaataaata taaatatcga tttcaaacaa tctggattag
    16921 gtagcgcttc gtgtggtcct atacctgaag ataaatacat tctaaaagta aattcgaaca
    16981 gtttcagttt tacattttct cctttccaag attcnnnnnn nnnnnnnnnn nnnnnnnnnn
    17041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17101 nnnnnnnnnn nnnnaattct tctttaattg ctagtcttga acttaaatca ttactgtttt
    17161 ctgcatctgt cagcatgtga tttggttgaa gtaatgctgc aagaattgct tttcttgtat
    17221 ttctcagtcc tgttacccat gccattactc tatttatgga agcatcaaag taatctgtag
    17281 ctacataagt gctcttcaat ttattcaatt taactaattc ttcaaaaatc gctttggttt
    17341 cgtcatcaaa tgatacaaca tgatcagaat cccatcttac aggtctgctt acatgaagta
    17401 ataaagactt ctgaaatgta aagattcctg aaagtttagc tgatactact tctgtcggat
    17461 gataatgtcc tgtatcaaga gtaagaataa tatgattttt cttcttattc attacatatc
    17521 ccatacaaaa ctcataacta ccagtagtat aactttcgag acctatacca aataatttac
    17581 tttcaagacc atctacaaca cctttaacat ccgcagtttc tttgaatatc ctgtcatagc
    17641 tatcttttag cctcaatctt ggtgcgagcg tatcatgagg aacttctttt gaaccatctg
    17701 aagtccagtg atttattacg catgtttttc ctgttctttc ataaaaatac tgacctatcc
    17761 ttctacatgc aataccatga ttgatccaaa attctcttat tttatcgtca acagctgaca
    17821 atgtcccatt atcacttttt ggatgagaaa aatatgtagg attgaaatcc agtcctattt
    17881 ttctatctac agcccaatct gcccattttt taaaatgctc aggttttatc tcatctctgt
    17941 caacaaaatc ttcagcttcc tgataattag catgtaaatt aactttttta actccaggaa
    18001 tgtatttcat tgcaaaatcc aaatcattac gtaattcttc tgcatttctt gctttgccta
    18061 aataattccc tgtaacctga attcctccgg taaggtcttt agaagatttt tcaaatcctc
    18121 ctacatcatc cccttgccag caatgaatgg atatagggat agaatcacat attttaattg
    18181 ctttttcggt gtcaatacca tatcttttgt aatattcttt agcctgctta tatgcttttt
    18241 cagtattgta cataatcatc ctcctcaata tttctatttt accatttatt aaatactttt
    18301 tcagcaaatc caagtaaatc tttaactctt attatcttgt tatcatcaag tactacttca
    18361 cctgtaaatc ttccgaacac ttgatgctga tcgcttttta ttataccaag tgatgtacag
    18421 gaagacctgt caagtactgg ctcgaaactc attgtgaaac ggttatcatt gctaataaat
    18481 ttccaaggtg aaagaaaatc atcttttccg tctttttttg gtattacaaa cttaactctg
    18541 tcaagtttat gagcttttcc attgtaaatt atcatattct ctgaagcagc tgatgtatca
    18601 ccaaaaccat atcctatatt gaatccaaat ggaatattgt caatatatcc tgaagctgaa
    18661 ccccagtacc atgtgttttt atatgtccac actcctctac cccaatcaag aacagcaaat
    18721 gaatcattct ttttaaattc ataaatgttt ccgttaaatt taacaaaccc ttctgctttc
    18781 atacaattaa ttttctgatt gtaataaaag gcgagtttat cttctttata tggtgtagca
    18841 ataaccattg attctttggg ttcatcagtt aattcaattt ctgcatcaaa ttgaatattt
    18901 ttcataaaat catcaagatg cgctgataaa attcttttct ttacatcgtt aataaactta
    18961 atggtgtaac ttttacctga actgatggtg tcaccattta atgagctgct cggcaaactt
    19021 tttattcctt ttgtcataaa cgacatatat gattttgtat gctgccattt attgttgaag
    19081 tctataaagg atactgaatc aagtcccata tatgagttgt cagctattgt aagagctaat
    19141 gcgaacttat cattgtttat gagataataa tcccattcct taatccttaa agaattagct
    19201 ttgatatcac ttcttaaata atcaaataga aacttcttgg catatcctgg ttcagtcaaa
    19261 acaccatctt catttaaaag ccgctttttc tcggttattt cgtgttgcat catttatccc
    19321 ctttcacatt tgattttagc atatattaac aaaaaagaaa ccctttacga tttctttcat
    19381 attatcgata ataattgtta acttacatca tagtatattg ttgattacgt taaattttag
    19441 tactaaagct acttattgcc ttttttggag acttttaact taggttcaat ttctccagct
    19501 ttagtcaatg caatctttgt aacaacaggt ccaatcaatt catatataag cgttgcacaa
    19561 agtataactg ttcttatgga atcaccatat tgtggaagtt gcatcataac tatctgagcc
    19621 ataccgacag cgacacctgc ctgaggtaat aatgctaatc caagatattt ggtgacatta
    19681 ggatgagcat gtaccatctt cgaacctgca aatgcaccaa aatatttacc tatcgaacgt
    19741 gcaactagat atagtatgcc gataactcca actgaaatta tcattttaac attgagttca
    19801 gcaccagaaa taacaaagaa taacaagaaa acaggcggag tccatctatc atatgcttcc
    19861 attacaggat cattattttt acagatgttc gcatacatag ctcctatcat catacaagta
    19921 agaagagctg ataactcaag aagctgactt aacgctacac caccaaatgt tattgcaaca
    19981 attagactga ttctgtttgc tcttgattta aagaatttca ttgataatga taatataaat
    20041 ccaagtaccg cacctattat taaagacatg gaaatttcat aaagaggtat caacacactt
    20101 gttttgaatg ttaatgcttc atgatttgca agtgcttttg ctattgacaa agaaattgaa
    20161 aatataatta aaccaattgc atcatctccg gcaacaacag gaagtagggt ttgagtaact
    20221 attccctttg ctttatactg ccttactacc atcaaagtag ctgcaggagc agtagcagtt
    20281 gcaattgctc ccaaagtcag aattataggt gcatcaagac cgggaatgaa aaaacttgct
    20341 ataaaaagaa gtaaatcaac tacaagtact gtcatcaatg cctgaacaac agtaataact
    20401 acaacttttt ttcctaattt ctttaaatgc tcaattttaa attcgccacc tatagaaaaa
    20461 gcaataaatc caagcgcaac agttgttatg atagaaaaac cttcaagcaa ttctgttgga
    20521 ataaatttaa agcagaatgg cccaataaga agtccagcaa ttatatatcc tgtaacatta
    20581 ggtaatccga atattttgac caaacgcgtc attaataacc ctgccagcat ggcaagacat
    20641 aagcttagta atatgttcat ttatttatac catagttatc ccttctgcaa aagatatcgg
    20701 tattgtaaac ataatacccg tatttggttt ttcaaggcct cctgtaacct gattaactat
    20761 tctttttgct atttctattt gttcatcagg aagtaccata aatactgtct tactactttc
    20821 acgtgaagga tttataaatt ctcttaatga accaaatatt gaaggtggtt ctattgattg
    20881 ttcacttata acttgtatca taccagtaga ttccagtaca gttgctccac agatattatt
    20941 ctccatcatt tcattgagta atttgcttaa aaattcagtt ttattcaaaa ttataactaa
    21001 aagttgcatt atctcttctt tctgcaaagc ttacgccttg actacttttg atattctaac
    21061 tctaatattt aaaatatgca agtattttat ttttcaccat aaagtctttt tgatgctctt
    21121 gtcgcttcaa atttcacttt ttgttcatca atggaagtta actctctgtt ctgcatcaat
    21181 atttctccat ttactaacac tgtatctaca tctgttgctc ttgcactata taccaaagca
    21241 gaaacaggat catgatatgg agtcatatta atactgtcag tgttaacaat tatcagatca
    21301 gctaacatgt cttttttgat aataccgcaa tcatcaaatc ctaaagcttt tgatccgttt
    21361 gttgtagcca ttttcaatat ctgtttagca cctatcgcag aactgttatg cttgtatcct
    21421 ttgtgaataa gtgctgcaag atgcatttcc ttaaacatat caagtgtatt attactagca
    21481 gcaccatctg tacctagtgc aggtgtaata cccatttcta tcattttcga tatatcagca
    21541 aatccactgc ctaacagcat attagatgat ggattatgta ctggtataac ttttctgtct
    21601 cttaataaag ccatatcgtc atcattcaca tgaactgtgt gagcagctgt taccttattg
    21661 ctaagtattc ctgttttttc acaaacttgt ataggtgtta atccatattt ttttacacaa
    21721 ttttctactt ctgtttcagt ctcatgaaga tgaatatgaa taagtgtatc agtttccttt
    21781 gccatttgag cacttaatac cagttctggt tcatcaacaa gatatacaga atgaacttct
    21841 attgaagtat taactcgacc tttaaaagcc ttcttgttat tgattacgaa atttttacaa
    21901 tattcctctg ctccttgttt tcctaattct ttaactttac ttgatgtaga tacaccatat
    21961 gagagattcg ctctaattcc tgtttgagag attgcttcta atgaagcttc atgaatgtaa
    22021 tacatatcaa gatatgatgt aattccattt ttaatcattt ctgtaatacc aagcatagcc
    22081 ccagagtaaa catcattata ggttagatta gcctcaactg gaaatattcc ttcacttaac
    22141 catctttcca aaggaaggtc atttgacctg tttctcataa gagtcatggc acagtgggta
    22201 tgagagttga ttagtccagg cataactatt ttaccttttg cgtttattat ccggtttggc
    22261 aagaagaaga atggttcatc ttctccaata tatacgattt tattatcttg tatgcagata
    22321 acctgattat tcaatatggt gaattcatcg tccatagtaa ggatatctgc attctttatc
    22381 attgtgttca ttagcacctc gcaaaaaatg gaatataaat attatattcc atttatgaat
    22441 ttatttccat taatcttaaa tcat
//
LOCUS       DAOSXM010000029        21371 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2111
            NODE_1565_length_21371_cov_11.008304, whole genome shotgun
            sequence.
ACCESSION   DAOSXM010000029 DAOSXM010000000
VERSION     DAOSXM010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864178
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 21371)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21371)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:24:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,261
            CDSs (total)                      :: 2,229
            Genes (coding)                    :: 2,218
            CDSs (with protein)               :: 2,218
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 3 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21371
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1565_length_21371_cov_11.008304"
                     /isolate="AMR_MDS_2111"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(98..463)
                     /locus_tag="PLQ68_05545"
     CDS             complement(98..463)
                     /locus_tag="PLQ68_05545"
                     /inference="COORDINATES: protein motif:HMM:NF024645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HXK72147.1"
                     /translation="MKKFSTLFDASVFASSKCSYWKFSTSDDYYDKISLPEIAKVHDQ
                     ESIADEDSYYVVADSGAIGFCEDEDTDIDWLFLVRDNLDESLPLTYVAKTVNKFCSNC
                     GRAVKSDARFCTDCGSKLG"
     gene            complement(472..1143)
                     /locus_tag="PLQ68_05550"
     CDS             complement(472..1143)
                     /locus_tag="PLQ68_05550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HXK72148.1"
                     /translation="MFCHNCGTKLDKKTAFCHNCGVKQENSKAEYVKPQNLIGFSERY
                     WDPSIIEAAKKHKRTSIGCMWSLVIIPVVGFILAGILIKEFDVKEAIIIGVSISVVML
                     IINLFGFARSKKPIWEGQVIDKSKKERQRHRNSGDDIETYTEYTTVIRTASGKKKTIT
                     ERDSQRHMYDYLKMGDRVRYYPVFDTFEKYDKSKDKIIYCNVCSMMNPISNDKCKRCN
                     NLLFK"
     gene            complement(1314..10568)
                     /locus_tag="PLQ68_05555"
     CDS             complement(1314..10568)
                     /locus_tag="PLQ68_05555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72149.1"
                     /translation="MKEKRSILVITIILTILLLAISAYAYYEYYGKYSALIQINGVIR
                     LDAFGALDDEYRGEENGYGTTPENPFIVDSKERMNNLIVLNNDGRLLDAKAVYGGAEK
                     FYFAFDFTEQPTPQVLDLTGVVQNPVGNNEYPFIDDLTGLLYAYLVSESEYVYMSAAA
                     SVDLEVQGSDVYIDEQLVTISSGSPSTGEYVKVPAFYYSTYPSIATQCLVTSDNNYYM
                     PVNLLLPVPQIVANSTIKAPYDQIDVGFFSCIGREYGILNDVINEEDIVSRSYVHDVI
                     LYNITIDCTEEQPNTIKAAFDAIWSTIMNSVPRHIFADTENQSDKRHIGLFTGHIDGM
                     VENITVAGEGVIKIDGADLNTYSKYTTVGYIDSRAIINGLQFSEMSGSGTGMGLVSQC
                     LFADSIYDLATNQAGVYEYQLDTVPGQSGIWSGVTTSVQGISSFSQGVFRFTLSESND
                     TVRDIWQGDGTINLLNEAGFNVTASVLYCSDEYRYSETPQGGGSLISTGASSNETRYA
                     GISPLSASSSILDKGKYIIVAKVFDNAEAVFKYYAVKIIAIIGQDGTIDYVFDDSEKV
                     DVTDYINGVSQSGIYTSCIWQTASDTQTPTFQNTRFKTEFLSVEQGSSLEMTLTDDSN
                     LAAAFSASVLNSTFTYTVTQQVGAGYVTTRYYLNFDTETSSFYFSTHNDTTIEIYQIS
                     NGFNLELVDNVSDITSNDDYIIVAQSGGSNYLIGAGTTEGIGGSMVVDGSFASDLSLS
                     SIPANWNINEYQAVRRYVWNAPYVSSGVVAFREKLSATYYLGISGSSLELSPQQNNWT
                     YAQNAGAGGSLNISGNYLSYLSNPGGSGFTLSTSPYTIYIYKLIPDDTEPDYNTYQGA
                     KLISSDSSIVQQGQYMIAVNTGTAYQAITMTASNTFGIADVTSYVNGTASSAQLQLLL
                     DGGANEGYKWNVVSTSSTPSLQNALYSSYLTNSSNLQLDLSVSPVEWMYDAVSGRLYY
                     SSGGTIYYAAYNGTGFEITSNAEDPALIYDIRLYRVTYEYEYSDVVPVQSISYTDSTY
                     IDTVYAGENKHYFILTAPLSDMENTAPYILGSFGTADQAPVESLNIAPTSTYDSLNSI
                     FTTTTDLSYYRWRLDTRMIDPTTAYETGYQFRNDVTDMYLGASNNPSSRTLVMVLNSN
                     INMDRTSTSASLENNDDSASPTGIGLGATSISRSPGPLYRTDGSGNYIGNSFFIMGRR
                     SPFEYYVWKTPSPGVFALALNNDTTAAPAEYTLPYLYEASGYTTNVIVSEVIEIGDNL
                     DEDMHYMITSVVNNLDSTVSYYSLSQGFDELSNKVLFGTNVTSQAEAINNSNILDEFG
                     QVIEESSTDMLIMVPVESDWYQTSSEKSLNFYQDYYSSNSVEDYLTASGSSISITSIP
                     VGSGTQPTDWYYDAISKYLIYTDGLDFYYLTYDILTDTFSLTTDRSSATHTNVYRFKP
                     TYVVSRVTDASNDSLIDGDFIIAGRDAEGYTSLGISDSGLSLEARNISEYIKETLTET
                     ERNEILNYIWKQQYYDFYSDPYNPVSTTQYMQLFSYITGGGFTVIYDTSTGGMELSSD
                     PMIWRITNNNGVWSFINNRLNGLVAANSRGITFVGDTSRMTVADTAKSINRYLATGFD
                     YLYSASTSGYPIVLCDTNGNLVTSPVSGSQYLALVKNGGVNYAAIKNDNGAITYVSYT
                     ASTASDSDCLWTVSENVGGWTLINGGRYISAVTGGSITSSTTANGTWEINNSTGQMSY
                     STYNFPYYTNTYSIGMTTDTGTYSARLYNYTYSDGVSTLVPTTATGSNKVLIFRTDTT
                     YRALIINNRSFSYTTLNNVNIQTDGTITSSTKITGNALLTVSQSGAGITVEGKRYLTS
                     TGTTFSTSSPSGTTFYLDTKGYFYYSEVNPFTPYVTVTTAYNTLASSGMTVYMFEHNS
                     GVYTPVTDGISAGKTYVMVVYDELVYYLIGQDGSLLTRTFVGSTLSSGWESKVVSSTH
                     HISSVSGPVGNYLVFSTNSGFSHLTIVNDKLVLTGSQTTQWEYDYSGQYGSDSRLYSV
                     STVNLPADDLTVGSTNRIETTTSVTDVILYQAVDDGNGNYTLTSKTTTGMPASGSYVI
                     VIYKEGIYYAVRYTSSGIIGYNLGSSVLEESIISDLVWDVDASGFRKDVEGQIYYLRR
                     NNEGLYASTLDGATWVYTYNNVTAGFTATNSSMTPLYIFRVDKSSELDDVTDTISMLS
                     GKAKLTQSVSLLESSNYVIVGEVDENDDNITDRYYALSMTDIYNTKPIDVTDLMNLAL
                     GYTSSYITLFDAGVWNNKGTDIEVVLKNRGFDDAYYLTGAQGNLSDMTPQVELVDEQA
                     AIDLTNYKWRVYTFDDVYLLGYSDMTTGVEITYYMYFDPIELQFKLTSDYLTASSNQG
                     IVQLYQLGAMTETQPTFHSYVIEVADDNQTILSYPVKLVESQDDLQIFSETMTEAGTV
                     RTGEYLISAVSGDSYYTLTLSELMNMAYVDVNPYFSGNFDIDINGNYSISINNEYIWK
                     QTSDPSTVLNFTSSAVTGYYLYSSNANFIYDIENRQLSNGTYFLNFDLSSGFTFSTSP
                     SQIPINLYLLGQTGLETDLPGGDLSYNYFTQPLTTIGGTVDFTKFSFTKTHMPDLQRY
                     GIGYDGTIGSQDGWTLYNVEQILESADSSVFFSSGINATQDFTEMKDEFTQHTHSTEY
                     NETPVDISYYAPSGSASFVIDEASQENPVFVNVVVTTEFDNILLNPEYLRYLALWKIA
                     DVDLTTGQTSTLLTYGDGLDEVSNYTHTFMEKRNTPYAAIPLPNIYGSLASGASYAKV
                     DGQEYTLSAPEYSKDYFIAHTYVITDPGVYYLGASYGSVAITYLAIDNRALTEEGETS
                     GMGFEDLFTIDFCYGDIETSVSGMVPNEALGNLAYVGSEEWFQSNIYPQFIQGTVTNP
                     TDYLIINVNRTKSDIDDTSSVNFKAYTTAPIAPGILHINDNTTAQRLTRKVDFRVYCN
                     GLDSTDFNGFSDRLWVVDKDNEGYLTFSTILLTGAVEGKYYINVSQYDIDNVLTVDQP
                     VLTTTNRYKWSILGGYLYTQDGRYLIRTANGYELSYSTANAVGVYTYDGSLVQILDPL
                     DDTPYLLVAYYEGVNKCVSVNMSQ"
     gene            complement(10565..11434)
                     /locus_tag="PLQ68_05560"
     CDS             complement(10565..11434)
                     /locus_tag="PLQ68_05560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72150.1"
                     /translation="MRNIKSKMAILLSALSIVLLVSILVTVSVAWFYFPTTQNVTIYT
                     DINYEIDVDLYEYRSNLGYFVQSGLNAAQDTVVADSGVSTSFVEWGGVFYPTVPFTNY
                     YMLILTYPDSNYLNGYLEADLRMQLTSGMNGTEPNDKVLYTKIAYSIAPDANIDPAST
                     SAITQVQSVQYIQMFLNPAPVEPPIDAPLDYMNDDHLNISLLNLNSQQYTYSTIEDTK
                     CRLVIFLRIESDPTHIAQSIEDNNISVNAVQIVTNNIYTFTCNFRSIPYKTGIIGYDR
                     INASDYTKKGGLL"
     gene            complement(11431..12192)
                     /locus_tag="PLQ68_05565"
     CDS             complement(11431..12192)
                     /locus_tag="PLQ68_05565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72151.1"
                     /translation="MSNQFIKKGILALSALLVLIIACLYWFGNISTAWFREDRSSYMT
                     ANHIYSITQGEANNIASFKNDGEFDADLQFEVINGDFTERFIPGDVLFYTFIVSDIDV
                     STAQTYMMELSGMAAEAIGAGSDATSFVSNCRIESDTLVIAVLQEMGNEEDGYYYELY
                     PYDESGNAFYDSSNGLYSTGVDYIYTELNGLEVELQFTLPSGVAYPPETAGVADLLVI
                     VPIWYMDTGLNQNIEMDCSLTIAGCIMYPYTGGGQ"
     gene            complement(12192..12782)
                     /locus_tag="PLQ68_05570"
     CDS             complement(12192..12782)
                     /locus_tag="PLQ68_05570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72152.1"
                     /translation="MKKKKILKILYIVLAVMFVLSAIVTTAMILSFDKDYSCPKFGNY
                     IIISVNDSELSPKILKGSALISDLEYNEVNRGDYVIIASKSSDGFQYAARFVEEVEDE
                     YYTVSGTQNRGAFYVAKDSIIAVNKIQIYGLGGFINMMRTQSGFFTFVIIPLVIIIMI
                     QIVRMVLLVQEKKLKEKYETDKNKISNTDKTEEVRS"
     gene            complement(12787..13659)
                     /locus_tag="PLQ68_05575"
     CDS             complement(12787..13659)
                     /locus_tag="PLQ68_05575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72153.1"
                     /translation="MKKKLISAAIRLAFGLVAFAVVSYWWFSDAWFAQSSKVQTEGVE
                     LSAVVPLNIYISGSDDEQLSQSMTFSMANLEQLGFGNMYEYEQNVVLRPSSSSDGSTF
                     WFAKKVNSEGIAIQDVASQSTYSLVEQDDLAYYMEKTIYLAATTEAYDDVTSLDCYVS
                     NVTIEGLSLNQLYKAARISISAVDDLENETTVIYRYAADITDTEGEALPVVDAHNKSL
                     NDPSVAMGTYEFMSSGALPINLVCAQQGIISVKEVIVRVWFEGENAFAVRSLAGGGFS
                     FEIEFTLVDPATGE"
     regulatory      complement(13706..13789)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(13904..21151)
                     /locus_tag="PLQ68_05580"
     CDS             complement(13904..21151)
                     /locus_tag="PLQ68_05580"
                     /inference="COORDINATES: protein motif:HMM:NF037374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBG domain-containing protein"
                     /protein_id="HXK72154.1"
                     /translation="MYNKVRSSCLKRHLIDAFLYFNYRKIMAVLISIIAILLIIPTQN
                     VEASTGSLSMEQSFVSPNSSESFTVNNSDTYNVTFDITSVDMTNPILVINVPKGINIS
                     YYPTASNATLSPTLITSGAVTNQQDENGNAVLTYRFNSDMTAIGFNITLMPTYKLDET
                     TYTVSASYYDGETIISIQSKTVTITNNPTLKPNIMSMSNSKNSLIFNDEVSEYYVSCR
                     GYIYYYPSRANHYSYDYVEYTVPLPDESVPGLGSGSSFAAFEEGVSYTIGSGTTAYSV
                     IYYESYNYINSAGAIVGNGKALLYTLPSTHTFTSGNTSYTFDHNIYLKLTVTEPGNYS
                     KNIRPQVKANVDGADIVLLDHVSYPQGYSVYSSNLLGCEIKEYNISDFFYAQNSYTYS
                     YYGYYILPIEGNTKYISRIYNKTEYLITDVKMEYTYPQTLYIDKIEFNLSSSLTSTQP
                     STATVKYKTFTGGDTVYEAVLNEDVPQLQLSDNTDSIVWMEVVYDKVPHSTASRTLFY
                     AHMTNEEALSTSTQVTSQIISAQSGTLGVIPLQNILQHNFYLRSSYNINAWQALNRTT
                     INKGDTFALNFQFASTSAEVLNPEYYAIMPSTYIFMGYTPNAAYQGIDYELSIRDLGD
                     GTTLYGVKYNDNIKRNGNAIHTFTFKVGPAVPTSTVQTFKLPLASYFQHDGTYYPYRT
                     SYMFIDIHDYDNDGDTTDQLAYSIDSERTTVTLNAVSAMTAQSYLKSNVTEGENTTMQ
                     YRYNSSGSYKYYMYNGLQSGNDASGIVLDIKVPKQNSTITYNSVNYTSKFNADLTGPV
                     SLEGNVFTNSVVTYKIEGSEDYVSDVMGLWNQVTDIRIEIENGRSFPSSDSAIVEVPF
                     LISGFDNQTSSSDYAFFDIQMNYTLTSTGDTVHVSRTEPTRLQAAYTYISGTIYKDFN
                     ANGTKDDNEISRNYYVYLYEGLSAEGTLISTNFSNYTTGNYSVGIISPGTYTLKVIKD
                     NNEYYPGADSSLFNSQGEYTFTLGYPTIDQITGVNLGIIAPRTMSLNFSSVLLLEGTP
                     RIIVPSLSPSLLDGEGPVVYSSSDESVVMVSSSGVLTYVSDGSATITVTVPQLSGIAA
                     IEGESDVITKTISVICQKPGCNITAQPYASLSSTGTSITSHSIQLTRGLTSNQTLYYI
                     FNRAGYCNFGHSDSRQVTWTIHDAGTTGATISSSISTYNYGYATLSVSSSGTVVLKAS
                     ETWPHDEAIKPEDIYVTINVYGQYDVTLPSATGYNASSFMSSSPVNHNGTFDFYVSLL
                     AGYDQSDIVVKANGVVITPELGFYTISNITENKVVTVEGVIPNVYNVTLNANGGTIDS
                     NNVTSYTYSTSSSLPTADDISRAGYTFIGWTATNSSDDSEYITSISNTEIGDKHYYAK
                     YTAIPVEVPSVFTQSGSTVEYGYSSATVSINAEVAEGHAITGYQWYSCKQDKSDAVLI
                     TGTDSHEYSIPVGLDAGSYYYYCVITVTRADNSLIETFDSSVASFIVNKKPLTATAFA
                     NNKIYEGTPTAEGNISLLGIINSDTVSASGTFTFTDSNVADNITVNVTGITLTGADSN
                     NYSVNTTASANANISRKVLTASINDYSKIYNQPNPEFNIYVTGFVNGETASSASGYVA
                     PIASTTATDTSVVGTYPIIITGGQADNYSFDISDTASLNILKKTLIESVTANDKPYDG
                     NTITTGSISLDGIVGTDNVSASGTFNFADSGAGENKLVNVTDILLSGDDADNYEINSS
                     ATCSADISKIMLTATVNSYTKIYGQDNPVFDVNVTGFVNGETEQTATGYVTPVASTAA
                     DNTSVVGTYPISITGGQADNYSFDISHTASLNILKKMLTANVNNYMKVYGETNPVFEV
                     IVSGFVNGDTVDTASGYIAPTPSTIAEDYTEIGIYVISLSGGQADNYTFDLSDTGLIN
                     INERQIDFTVNAYDKEYDSLIGTSGIIILSGIVNNDMVYANATFTFDTIFPGSQKTVS
                     VTNIRLYGDDAHNYVLSSNTAATIANITYDNIDEDESDTESGIDVYVNGQSQTAAKAS
                     SEQIDGKKVTTITIEDKKIDDKLEKEGNNSVVTIQYNDSSDTLTSNLNGQTVKNMEQK
                     EAILEIKSLNITYSIPASDIDIDDVASKLDDVTSLSDIEVSITISNPAEDTVNLIQNT
                     ADENNYSLVVQPVQFDITCTSESGQIQVTKFNSYVERLIMIPEGVDPNKITTGIVMNE
                     DGTFTHVPTKIIIIDGIYYAKINSLTNSVYSIIWNVADLTDVKGHWAEDIINDMNARL
                     VVEAKTDDKFAPNTDITRAEFTDILIRGLGLLRAGTGKLVFDDVTEASKYFDSISIAY
                     DYGIIKGVGGDSFNQDKLITREHAMLMIYRAMKITNMDAILTATDFVRFNASFDDETL
                     YSPETVNSAVACFKYGIMEGKAYRVSDPLDNLSRAEAAVFIYRLLKISGLI"
BASE COUNT         6713 a         4122 c         3337 g         7199 t
ORIGIN      
        1 gattttttta atatcacaaa aatctgtaaa ccaatatatc cttttacaat tgattataac
       61 aagtaaccat cttaatatat tgttttagcg ttttatttta tcctaattta cttccacaat
      121 ctgtgcagaa tcgcgcatct gattttactg ctctaccgca atttgaacag aacttattta
      181 ctgtttttgc aacataagtt aaaggtaatg attcatcaag attatctctg acaagaaata
      241 gccagtctat atctgtatcc tcatcctcac aaaatcctat tgctccagaa tcagcaacta
      301 catagtaact gtcttcatca gcaatacttt cttgatcatg aactttagca atttctggca
      361 agcttatctt gtcataataa tcatctgaag ttgaaaactt ccagtaactg catttacttg
      421 aagcaaatac tgatgcatca aaaagagttg aaaacttttt cataacaccc cctatttaaa
      481 taataaatta ttacatcttt tacatttgtc gtttgagata ggattcatca tgctgcatac
      541 attgcaatat attattttat ctttggattt atcatatttc tcaaacgtat caaaaacagg
      601 atagtatctc acccggtctc ccatttttaa ataatcatac atatgacgct ggctatctct
      661 ttcagttatt gtttttttct tgcctgaagc agtccttatt acagttgtgt attcagtata
      721 tgtttctatg tcatcaccac tatttctatg tctttgtctt tctttttttg atttatctat
      781 aacttgccct tcccatatcg gcttttttga tctagcaaaa ccaaaaagat tgataatcaa
      841 cataaccaca gatatactta cccctattat gattgcttcc tttacatcaa attctttaat
      901 cagaatacct gccaatataa atcctactac tgggattatg accaaagacc acatacatcc
      961 aattgatgtt cttttatgtt tttttgcagc ttcaataata cttgggtccc aatatctttc
     1021 agaaaagcct ataagatttt gaggtttaac atattcagct ttgctatttt cctgttttac
     1081 accacagtta tgacaaaaag ccgtcttttt atcaagttta gttccacaat tgtgacaaaa
     1141 cataaaacac ccccatttat ctattggcaa acactttttc caccttcaca aaaacctgtg
     1201 aaatatttat gtcacaatat gaaactggat gtaagcacac tttaactgga aatattatat
     1261 cacaaatcat ataaaaatcg tctacaacaa ataaaacagc ccgtcataaa taactactgt
     1321 gacatattta cactgacgca cttattaaca ccttcatagt atgcaaccag aagataagga
     1381 gtatcatcaa ggggatcaag tatctgaaca aggctaccat cataagtata taccccgact
     1441 gcatttgctg ttgaatagga aagctcatat ccgtttgcag tccttataag gtatctgcca
     1501 tcctgcgtgt aaaggtatcc tccaagaatg ctccatttat acctgttcgt tgtagtaagt
     1561 actggctggt ctactgttaa tacattgtct atatcatact gagaaacgtt aatatagtat
     1621 ttaccttcga ctgctcctgt caacaatata gtagagaaag ttaaataacc ttcgttgtct
     1681 ttatccacaa cccataatct gtcactgaaa ccattaaaat cagtggaatc tagtccattg
     1741 cagtagactc tgaaatccac tttcctagtt aatctctgag cagttgtatt atcgtttata
     1801 tgcagtatac caggcgcaat tggtgcagtt gtatatgctt tgaaattcac gcttgatgta
     1861 tcatctatat ctgattttgt acgattaaca tttataataa gataatcggt tgggttagtt
     1921 actgtccctt gaataaactg aggatatatg ttgctctgga accattcttc tgaacctaca
     1981 taagcaagat tacctaaagc ctcattgggc accattccgc taacggatgt ttctatatct
     2041 ccataacaga aatctatagt aaacaaatct tcaaatccca tacctgaggt ctctccttct
     2101 tcagtaagcg ctctgttatc tattgctaga taagtaattg ctactgagcc ataggaagct
     2161 ccaagataat atactccagg atcagttatt acataagtgt gagcaatgaa gtaatctttg
     2221 ctgtattcag gagcgcttaa tgtatattcc tgcccgtcta ccttagcata tgacgctccg
     2281 cttgctaaac ttccatatat gtttggaagt ggtattgcgg catatggcgt atttctcttt
     2341 tccataaacg tgtgtgtata attagagact tcatctaaac catcaccata tgtcaacaaa
     2401 gtacttgtct gtcctgtagt taagtcaaca tctgctatct tccataatgc aagataacgt
     2461 aaatattctg gatttaaaag gatattatca aattctgtgg ttacaacgac attaacaaat
     2521 acagggttct cctggctggc ttcgtcaata acaaaggaag ctgagcctga tggtgcataa
     2581 tatgaaatat caacaggtgt ttcattgtat tctgtgctat gagtatgttg agtaaattca
     2641 tctttcatct ctgtaaaatc ctgtgtagca tttataccac tggaaaagaa tacagatgaa
     2701 tctgctgatt ctaatatctg ctcaacatta tataatgtcc agccatcctg actgcctatt
     2761 gttccgtcat atccaatccc gtatctttgc aaatccggca tatgagtttt cgtgaaactg
     2821 aatttagtaa aatcaactgt tcctccaatt gtagtaagcg gctgagtaaa gtaattgtat
     2881 gataagtctc caccaggaag atcagtttct aatccagtct gcccaagaag gtacaggttt
     2941 atggggattt gtgacggact ggttgaaaag gtaaatccgc tacttaaatc aaaattcaaa
     3001 aaatatgttc catttgataa ctgacggttc tcaatatcat atatgaagtt tgcattcgaa
     3061 gagtacaaat agtaacctgt taccgccgaa cttgtgaaat tcaaaacagt tgaaggatcc
     3121 gatgtctgtt tccatatata ttcattatta atggatatac tgtaatttcc gttaatatca
     3181 atgtcaaaat tcccgctaaa atatggatta acatcaacat aagccatgtt cataagttct
     3241 gataaagtta aggtgtaata actatctccg cttaccgcac tgataaggta ttctccagtc
     3301 cttacagtac cggcttcagt catagtttct gaaaaaatct gtaaatcatc ttgtgattca
     3361 acaagtttaa caggatatga caatattgtc tgattatcat ctgcaacctc tattacatat
     3421 gaatggaatg tcggctgggt ttcagtcatt gctcctaact gatataactg aacaataccc
     3481 tgattgctag acgcagtaag ataatcagaa gtaagcttaa attgaagttc aataggatca
     3541 aagtacatat aatatgtaat ttctacccca gtagtcatgt cactataacc aagaagatat
     3601 acatcatcaa aagtataaac tctccatttg taatttgtta aatcaattgc agcctgttca
     3661 tcaacaagtt caacctgagg ggtcatatca gaaagatttc cctgtgcacc tgttaagtaa
     3721 taagcgtcat caaaccccct atttttcaaa actacttcaa tatcagtacc tttattattc
     3781 caaacacctg catcaaataa agtaatataa ctgctggtgt aacctaaggc aagattcata
     3841 agatcagtca catcaattgg ttttgtattg taaatatcag tcatggaaag agcataatat
     3901 ctatcagtaa tattatcatc gttttcatca acttccccta caatgacata gttgcttgat
     3961 tctaaaagac ttacggactg agtcagtttt gcttttcctg aaagcattga aatggtatca
     4021 gtcacatcat caagttcaga actcttgtca actctgaata tatataaagg agtcattgaa
     4081 ctattggttg cagtaaaacc agctgttaca ttattgtatg tataaaccca tgttgcacca
     4141 tctaatgtgc tcgcatataa tccttcatta tttcgtctta aatagtaaat ctgaccttcc
     4201 acatcctttc taaacccaga tgcatcaaca tcccaaacaa ggtcagatat gatgctttcc
     4261 tcgagaacag aacttccaag attatatcca ataataccgc ttgatgtata tcttacagca
     4321 tagtatatac cttctttata aattactatt acataagaac cagatgcagg cattccagtt
     4381 gttgtcttgc ttgtaagagt atagtttcca tttccatcat caacagcctg atacaatatg
     4441 acatctgtaa cagatgttgt tgtctcaatc ctgtttgttg agccaactgt aagatcatcg
     4501 gcaggcagat taactgttga tacactatat aatcttgaat cagaaccata ttgaccgcta
     4561 tagtcatatt cccattgggt agtttgagaa ccggttaaaa caagcttatc atttacaatg
     4621 gtcagatgac taaatcctga atttgtgctg aacacaagat agtttccaac aggacccgat
     4681 actgatgaaa tgtgatgagt agacgaaaca actttacttt cccatccgct tgacagagta
     4741 gaacctacaa aagtacgtgt cagaagcgaa ccatcttgcc cgataagata atatactaat
     4801 tcatcatata caaccataac ataagtctta cctgcagaaa tgccatcagt aacaggagta
     4861 taaactcctg agttatgttc aaacatatac actgtcatgc ctgaggatgc taaagtattg
     4921 taagcagttg ttactgtaac atatggagta aagggattta cttctgagta ataaaaataa
     4981 ccttttgtat caagatagaa agtagtacca ctaggacttg atgtgctaaa agtagtacct
     5041 gttgaagtaa gatacctttt tccttctaca gttatacctg ctcctgattg ggaaacagtt
     5101 aaaagtgcat ttccagtaat ttttgtagat gaagtaattg ttccatctgt ttgaatattt
     5161 acattattta gagtagtgta tgaaaaagat ctattattaa taataagtgc cctatacgtt
     5221 gtatctgttc taaaaatcag aaccttatta cttccagttg cagttgtagg cactaaagta
     5281 ctgactccat cactataagt ataattatat aatcttgcag aatatgttcc tgtgtctgtg
     5341 gtcattccaa tactataagt atttgtataa tatgggaaat tatatgtcga ataactcatc
     5401 tgaccagtgc tattgttaat ttcccatgtt ccatttgcag tagttgatga agtaatactg
     5461 ccaccagtta ctgctgatat atatcttcct ccgtttatta gagtccaacc acctacattt
     5521 tccgaaactg tccataagca atctgaatct gaagcagttg atgctgtgta acttacatat
     5581 gtaatcgcac cattatcatt cttaatggct gcataattta cacctccatt ttttactaat
     5641 gctaaatatt gacttcccga aacaggtgaa gtgactaaat tcccattagt atcacataat
     5701 acaatagggt atcctgatgt tgaagcagaa tataaatagt caaatcctgt agcaagatat
     5761 ctgttgattg attttgcagt atctgcaacg gtcattcttg atgtatcacc tacaaatgtt
     5821 attcctcttg aattagctgc tacaagtcca ttcaatctgt tgttaataaa gctccatacc
     5881 ccgttattat tggtaattct ccaaatcata ggatctgagc ttaattccat tcctcctgta
     5941 cttgtgtcgt atatgaccgt aaatcctcca ccggttatat atgagaaaag ctgcatatat
     6001 tgtgttgtag aaaccggatt gtatggatca gaatagaaat cataatattg ctgtttccat
     6061 atgtaattca atatttcatt tcgttcggtc tcagtaagag tttcctttat atactccgat
     6121 atatttctcg cttcgagact taatccgctg tcagatatac ctaatgaagt atagccttct
     6181 gcatctctgc cagcaattat aaaatcgcca tcaatcagac tgtcatttga tgcatcagta
     6241 actctgctga ctacatatgt cggtttaaat ctgtaaacat tcgtgtgtgt tgcagaggac
     6301 ctatcagttg taagtgaaaa agtatctgtt aaaatatcat aagttagata atagaaatcc
     6361 aatccatctg tgtatattaa gtatttggat attgcatcgt aataccagtc tgttggctgt
     6421 gttcctgaac ctacaggaat acttgttatt gatatacttg aaccagatgc cgtcagataa
     6481 tcctcaacag aattactgga ataatagtcc tgataaaaat tcagactttt ttcactgctt
     6541 gtctgatacc agtctgattc aactggaacc attattaaca tatctgtact gctttcttct
     6601 attacctgtc caaactcatc aagaatattg ctgttattaa ttgcttctgc ctgagaagtt
     6661 acattggtgc caaatagaac cttattactc aattcatcga atccctggct taaagaatag
     6721 taactaaccg tactgtccag attatttaca actgatgtaa tcatataatg catatcttca
     6781 tcaagattat cacctatttc tataacttct gaaacaataa cattagttgt atagccagat
     6841 gcttcataca gatacggcag agtatattca gctggagctg ctgttgtgtc gttgttcaaa
     6901 gctaatgcga aaacacctgg tgatggagtc ttccatacat aatattcaaa tggtgacctt
     6961 ctgcccataa tgaagaaaga attaccgata tagttaccgc ttccgtctgt tctgtataac
     7021 gggcctggtg atcgtgatat tgatgttgct cctaaaccaa ttcctgtcgg tgaagcactg
     7081 tcgtcattgt tttccagact tgcactagta cttgttctat ccatattgat atttgaattt
     7141 aataccatta ccaaagtacg agatgatgga ttatttgatg ctccaagata catatctgtt
     7201 acatcatttc taaactgata gcctgtttca tatgcagtag tagggtctat cattcttgtg
     7261 tcaagcctcc atctgtaata cgaaagatct gtagtagttg taaaaatgct atttaaagaa
     7321 tcatatgttg aagttggtgc tatgttcaaa gattcaacag gcgcttgatc tgctgtacca
     7381 aaagaaccca gaatatatgg agcagtattt tccatatcac ttaaaggagc agtaagtatg
     7441 aaataatgct tattctcacc tgcatataca gtatcaatat aggtagagtc tgtatatgat
     7501 atcgattgca ctggaacaac atcagaatac tcgtattcat atgtcaccct atacagcctt
     7561 atatcatata tcaaagcagg atcttcagca tttgatgtta tctcaaaacc tgtcccgtta
     7621 tatgcagcat aatatattgt cccgcctgac gaataataaa gccttcctga tacagcgtca
     7681 tacatccact caaccggact gactgataaa tccaattgca gatttgatga attggttaga
     7741 tatgaactat ataatgcatt ttgcaatgat ggagttgatg atgttgaaac aacattccat
     7801 ttatacccct cattagcacc accatctaaa agcaactgaa gttgcgctga agaggcagtg
     7861 ccatttacat aactggttac gtcagcaatg ccaaacgtgt tagatgctgt cattgtaata
     7921 gcctgataag cagtgcctgt atttactgct atcatatatt gtccttgctg cacaattgag
     7981 ctgtctgaag atataagctt agctccctga taggtattat aatcaggctc ggtatcatct
     8041 ggtataagtt tgtaaatgta aattgtatac gggcttgtag acaatgtgaa accagatcct
     8101 ccaggattag ataaatatga taaatagttg ccacttatat ttaaactgcc tcctgcacca
     8161 gcattttgag catatgtcca attattctgc tggggtgata attccaatga acttccagaa
     8221 attccaagat agtatgttgc acttaatttt tcacgaaaag ctacaactcc tgatgataca
     8281 taaggtgcat tccatacata tcttctaact gcctgatact catttatatt ccaattagct
     8341 ggtatagaag ataaagacaa atctgaagca aatgatccat caacaaccat cgaaccgcct
     8401 ataccttctg tagtccctgc tcctataaga taattactgc ctccactttg agcaacaata
     8461 atatagtcat catttgatgt tatatctgaa acattatcca ctaattccag attaaaccca
     8521 ttggatatct gataaatttc aatagttgta tcattatgag ttgaaaaata gaaagatgat
     8581 gtttcagtgt caaaattcaa atagtatctg gttgtaacat atcctgctcc tacctgctgc
     8641 gttactgtat atgtaaatgt gctattaagc acgctagcgc taaatgctgc tgcaagattg
     8701 gaatcatctg tcaaggtcat ttctaaagaa gacccttgct caacagataa aaactctgtt
     8761 ttaaaacgag tattctggaa agtcggggtc tgtgtatctg atgctgtctg ccatatacaa
     8821 cttgtatata ttcctgactg gcttactcca ttgatatagt ctgttacatc aaccttttca
     8881 gaatcatcaa acacataatc tattgttccg tcctgtccga taatagcaat tatcttcact
     8941 gcataatatt taaaaactgc ttcagcatta tcaaatacct tggcaacaat tatatatttt
     9001 cctttatcta gtatactgct cgatgcagac aatggactta ttcctgcata tctggtttca
     9061 tttgaagatg ctcctgtaga tatcagactt cctccgcctt gaggcgtttc gctgtatctg
     9121 tactcatcag aacagtacaa tactgaagca gttacattga atcctgcttc attaagcaga
     9181 ttgatggttc catctccctg ccatatgtct ctcacagtat cgtttgattc agaaagagta
     9241 aatctaaata cgccttgaga aaagctgctt ataccttgaa ctgaggttgt gacaccgctc
     9301 catattccag actggccagg aacagtatct agctgatatt cataaactcc agcctgattg
     9361 gttgcaagat catatattga atctgcaaac agacactgtg aaactaaacc cattcctgtt
     9421 ccagatcctg acatttcaga aaactgcaat ccattaatta ttgctctgct atcaatatac
     9481 ccgactgtag tatactttga atatgtatta agatctgcac catcaatttt aattacacct
     9541 tcaccagcta cagttatatt ctcaaccatt ccgtcaatat gacctgtaaa caggcctata
     9601 tgccttttat cagactgatt ttcagtatct gcaaatatat gtcttggaac actgttcata
     9661 attgtgctcc atatagcatc aaatgctgct ttaatcgtat taggctgttc ttccgtacaa
     9721 tcaattgtaa tattgtataa gataacgtca tgaacataag aacggcttac tatatcttct
     9781 tcattgatta catcattaag tattccatat tcccttccta tacagctgaa aaaaccaaca
     9841 tcaatctgat catatggtgc tttaatagta gagtttgcaa ctatctgagg cactggaaga
     9901 agcagattga ctggcatata gtaattatta tcagaagtaa ctaaacattg tgtggcgata
     9961 gatggatatg ttgagtaata gaaggcaggc accttgacgt attcacctgt tgatggtgaa
    10021 ccggatgata ttgttacaag ctgttcatct atatatacat cagaaccctg aacttctaaa
    10081 tctaccgaag ctgcggcact catataaaca tattctgact cactaactag atatgcatat
    10141 aaaagacctg ttaaatcatc tataaatgga tattcattat ttccaaccgg gttttgaact
    10201 actcctgtta aatcaagaac ctgcggtgta ggctgttcag taaaatcaaa cgcgaaataa
    10261 aatttttccg ctcctccgta cacagctttt gcatctaaaa gccttccatc attattaaga
    10321 acaattaaat tattcatcct ttctttgctg tcaactatga aaggattttc cggagtcgtt
    10381 ccgtatccgt tttcttcccc acgatactca tcatccaatg ctccaaatgc atccagcctt
    10441 ataactccgt taatctgaat aagggcgctg tatttgccat aatactcata ataagcatat
    10501 gcacttatgg ctaatagtaa aatcgttaat attatggtta ttactaaaat actgcgcttt
    10561 tccttcataa cagacctcct tttttggtat aatctgaagc atttattctg tcatatccga
    10621 ttatgcctgt cttatatggg atacttctga aattacacgt aaatgtatat atattatttg
    10681 tcactatctg aacagcatta accgatatat tattatcttc aatagattgc gctatatgcg
    10741 tcggatcact ctcaattcgc aaaaatataa ccaatcggca ttttgtatct tcaatagttg
    10801 aatatgtata ctgctgagag ttaaggttta gtaatgagat attcagatga tcatcattca
    10861 tatagtctaa aggagcatca atcggaggtt ctacaggtgc aggatttaga aacatttgta
    10921 tatactgaac cgattgtacc tgtgttattg ctgatgttga agctggatca atattagcat
    10981 caggagcaat agaatatgct atttttgtat acagcacctt atcattaggc tctgttccgt
    11041 tcattcctga tgtcagctgc attctaaggt ctgcttcaag atatccgttc agataattac
    11101 tgtctggata tgtaagtatc agcatgtagt aatttgtaaa cggtacagta ggatagaaaa
    11161 ctcctcccca ttctacaaaa gacgttgata caccactatc agcaacaacg gtatcttgtg
    11221 ctgcattcaa tcctgattgc acaaaatatc ccaaattact tctgtattca tataaatcta
    11281 catcaatctc ataatttata tcagtatata ttgttacatt ctgtgtagtt ggaaaataaa
    11341 accaagctac acttactgta actaaaatgg aaaccaataa aactatactc aatgcgctta
    11401 gaagaatagc cattttgctt ttaatgtttc tcactggcca cctcctgtat aaggatacat
    11461 aatacatcct gcaatagtca aagaacaatc catttcaata ttctgattca atccagtatc
    11521 catataccag ataggaacta ttacaagcaa atctgctact cctgctgttt caggaggata
    11581 tgcaacacca gatggcagag taaactgcag ttctacttcc aaaccattta gctcagtata
    11641 tatgtagtct acgccagtgc tgtacagacc attacttgaa tcataaaagg cattgccgct
    11701 ttcatcataa ggatatagtt cataatagta tccgtcttct tcattaccca tttcctgcag
    11761 tacagctatt acaagagtat cagactctat tctgcaattg cttacaaaag atgtagcatc
    11821 tgaacctgca ccaattgctt cagctgccat tcctgataac tccatcatat atgtctgtgc
    11881 agtagagaca tcaatgtctg aaacaatgaa tgtgtaaaaa agaacatccc cgggaataaa
    11941 tctttctgta aaatccccgt taataacttc aaattgaaga tctgcatcaa attcgccgtc
    12001 atttttaaat gatgcgatgt tgttagcctc tccttgtgtt attgaatata tatgatttgc
    12061 tgtcatatat gatgatctgt cctctctgaa ccacgcagta gatatattac caaaccagta
    12121 taaacatgcg attataagca ctaataatgc agataacgcc agtattcctt tttttataaa
    12181 ctgatttgac attaacttct tacctcttct gttttatctg tgttacttat cttattttta
    12241 tcagtctcgt acttttcttt aagtttcttt tcctgaacaa gcaaaaccat ccttactatc
    12301 tgtatcatta ttattatgac aagaggaata atcacgaaag tgaagaaccc tgactgagtt
    12361 ctcatcatat ttataaatcc gcctaagcca taaatctgga ttttgttaac agctataata
    12421 ctgtcttttg caacatagaa tgcacctctg ttctgtgtac ctgaaactgt ataatactca
    12481 tcttcaactt cttctacaaa tcttgcagcg tattgaaatc cgtctgaact ttttgaagca
    12541 attatgacat aatcgcctct gttaacttca ttatattcca aatcagatat cagggcactg
    12601 ccttttagta tttttggcga taattcagaa tcatttacgc tgataataat gtaatttcca
    12661 aacttcggac atgaataatc cttatcaaaa cttaatatca tagctgtagt aacaattgct
    12721 gatagtacaa acataacggc taaaactata tataagattt taaggatttt tttctttttc
    12781 atttatctat tctcctgtag caggatcaac aagcgtaaat tcaatttcga atgaaaatcc
    12841 tcctcctgca agactcctta cagcaaaagc attttcacct tcaaaccata ctcgtactat
    12901 aacttctttc acactaatta taccctgctg ggcacaaaca agattaattg gtaatgctcc
    12961 ggaactcata aattcgtatg ttcccattgc aactgaaggg tcgttcaagc ttttattatg
    13021 agcatccact actggtaatg cttctccttc tgtatcagtt atatctgcag catatctata
    13081 tatgactgtg gtttcgtttt ctaaatcatc cactgcagat attgatattc ttgcagcttt
    13141 atataactga ttcaaactaa gtccctctat agttacattg ctaacataac aatccaatga
    13201 ggttacatcg tcatatgctt cagtcgtagc agctaaataa atagtctttt ccatataata
    13261 tgccagatca tcctgttcaa ctaatgagta tgtagactgg ctggcaacat cttgaattgc
    13321 tatgccttcc gaattaactt tttttgcaaa ccaaaaagta cttccatctg atgacgaaga
    13381 aggtcttaaa actacattct gttcatactc atacatattg ccaaaaccaa gttgttctag
    13441 atttgccatt gaaaaagtca tactttgcga aagctgctca tcatcacttc cagaaatata
    13501 aatatttaaa ggaactactg ccgataactc aactccttca gtctgaacct tactactttg
    13561 agcaaaccat gcgtctgaaa accaccagta agaaactaca gcgaaagcaa caaggccaaa
    13621 agccagtctt attgctgctg aaattaattt tttcttcact gcaatttcct cctaataaaa
    13681 aataaaggta gcctgtgcaa atactcattg caactggctg ccttttaaac tcgtttacgg
    13741 ccctatagtt ttgcgtcaca ccctttcagg tgttttgcct ttctagtgaa gttcataata
    13801 tcatagtatg taaattttgt aaataaaatt tttactaatt aatattcttt atacaatcaa
    13861 atctgcaata ccttaataag ttaatgcata attgcagttt ttgctatatt agacccgaaa
    13921 tttttagcag tctgtatata aatactgcag cctctgctct tgataaatta tctaatggat
    13981 ctgatactct gtatgccttt ccttccataa ttccgtactt aaagcatgct acagcagaat
    14041 taactgtctc aggtgaataa agagtttcat catcaaatga agcattaaat ctaacaaaat
    14101 cagttgctgt aagaattgca tccatatttg ttattttcat tgctctgtaa atcataagca
    14161 tagcatgctc tcttgtaatt aatttatctt gattgaaaga atctccacct actcctttta
    14221 taatcccata atcatatgcg attgatatac tatcaaaata tttactggct tcagttacat
    14281 catcaaacac tagcttacct gtacctgctc tcaataaacc aagtcctctt attaagatat
    14341 ctgtaaactc tgctcttgtt atatctgtat ttggagcaaa tttatcatca gtctttgcct
    14401 caactacaag tcgcgcattc atatcattga taatatcttc tgcccagtgg cctttaacat
    14461 cagttaaatc ggcaacattc caaattattg agtacacact gttggtcaaa ctgttaattt
    14521 tggcataata tatcccatcg ataattatta tcttagttgg aacatgagta aatgtaccat
    14581 cttcattcat aacgattcca gtagtaatct tatttggatc gacaccttct ggtatcatta
    14641 ttaatctttc aacataactg ttgaatttag ttacctgtat ctggcccgat tctgatgtgc
    14701 atgttatatc aaattgtact ggctgcacta ctaaactgta attattctca tctgctgtgt
    14761 tttgaattaa attaactgta tcttcagcag gattgcttat agtaatgctt acttcaatat
    14821 cactcaggct ggttacatca tctaattttg atgcgacatc atcaatatca atatctgaag
    14881 caggtatgga ataggtaata tttaaggatt ttatctctaa aatagcttct ttttgctcca
    14941 tattctttac tgtctggcca ttgagattac tagtaagagt atcagaagaa tcgttatact
    15001 gaattgtaac tacagagtta ttaccttctt tctctaattt gtcatcaatt ttcttatcct
    15061 caatagttat agttgtaact ttcttcccgt caatctgctc agaagatgct ttggctgcag
    15121 tctgtgactg gccattaaca taaacatcaa ttccactttc agtatcactt tcatcctcat
    15181 ctatattgtc atatgttata ttagcaattg ttgcagctgt gtttgatgat aatacatagt
    15241 tatgcgcatc gtcaccataa agtcgtatat tagttacgct tactgttttt tgacttccgg
    15301 ggaatattgt atcaaatgta aatgtagcat ttgcatacac catatcatta tttacaatac
    15361 cactgagtat tatgattcca ctggttccta taagagaatc atattctttg tcataagcat
    15421 ttactgtaaa atctatctgt ctttcattta tatttatcaa acctgtatca gataaatcaa
    15481 aagtatagtt atcagcctgt cctcctgata aactaataac ataaattcct atttcagtat
    15541 aatcttcagc aattgtagaa ggtgtaggtg caatataacc tgaagctgta tctacagtgt
    15601 ctccattcac aaagcctgaa actataactt caaaaactgg attagtttca ccatatacct
    15661 tcatgtagtt attaacatta gcagtaagca tcttttttaa tatattcaag cttgctgtat
    15721 gagaaatatc aaatgaataa ttatctgcct gaccgccagt tatagaaatc ggatatgttc
    15781 ctacaaccga tgtattatca gcagcagtag acgcaacagg agtaacatat ccggtagcag
    15841 tctgttccgt ctcaccattt acaaaacctg tgacatttac atcaaacact ggattatcct
    15901 gtccataaat ctttgtatag ctgttaactg tggctgtaag catgattttt gatatatccg
    15961 cactgcatgt tgcactgcta ttaatttcat agttgtctgc atcatcacca gaaagtaata
    16021 tatcagttac attaacaagt ttgttttcgc ctgcaccaga atctgcaaaa ttgaaagtac
    16081 ctgatgctga aacattatct gtccctacta tcccatcaag tgaaatgcta ccagttgtaa
    16141 tagtgttacc atcatatggt ttatcattag cagttactga ttcgatgaga gtttttttaa
    16201 ggatattaag acttgctgta tcagatatgt caaatgaata attatctgct tgaccaccag
    16261 taatgataat tgggtatgtt cctacaactg atgtatctgt tgctgtggta gatgcaatag
    16321 gagcaacata acctgaagca gaagatgctg tctcaccatt aacaaatcca gtgacataaa
    16381 tattgaactc aggatttggc tggttatata tctttgaata atcatttatt gatgcagtaa
    16441 gcacctttct acttatattt gcatttgctg atgctgttgt gttaacagaa taattatttg
    16501 aatctgctcc tgttagtgtt atacctgtta cattaacagt aatattgtca gctacatttg
    16561 aatcagtaaa tgtaaatgta ccacttgctg aaactgtatc agaattaatt atacctaaca
    16621 gtgatatgtt accttctgca gtcggtgttc cctcatatat cttattgtta gcaaatgctg
    16681 ttgcggttaa tggcttctta ttcactatga acgaagcaac tgaggaatca aaagtctcta
    16741 ttaaagagtt atcagcacga gtaactgtaa ttacacagta atagtaatat gaacctgcgt
    16801 ccaagccaac aggaattgaa tactcatgtg aatctgttcc agtaatcaaa acagcatctg
    16861 atttgtcttg tttacatgaa taccactggt atccggttat agcatgtcct tctgcaactt
    16921 cagcattaat gctcactgtt gctgatgagt aaccatattc cacagtggaa ccactttgag
    16981 taaatactga aggtacttca acaggaattg ctgtgtattt tgcataataa tgcttatcac
    17041 caatttccgt gttacttatt gaagttatat attcagaatc atcagagctg tttgtagctg
    17101 tccaaccaat aaaagtatat cctgctctgg atatgtcatc tgctgttggt agagaactgc
    17161 ttgtgctata tgtatatgaa gttacattat ttgaatcaat agttccacca tttgcattca
    17221 gtgttacgtt atatacatta ggaattacgc cttcaacagt cacaacttta ttttctgtga
    17281 tattggaaat ggtataaaat cctaattctg gagtaatcac aacaccattt gcttttacaa
    17341 caatatctga ctggtcgtat cctgctaaaa ggctaacata aaaatcaaag gtaccattgt
    17401 gattaacagg acttgacgac ataaatgaag atgcattata tcctgtagcg gaaggcaatg
    17461 ttacatcata ttgcccatat acattgatag taacataaat atcttctggt tttattgctt
    17521 catcatgagg ccatgtttct gaagctttaa gtactactgt accagaagat gatactgaca
    17581 aagtcgcata tccataatta tatgtcgata tacttgatga aattgttgca cctgttgtac
    17641 ctgcatcgtg aatggtccat gtaacctgtc tactgtctga atgcccgaag ttacagtatc
    17701 ctgctctgtt aaagatataa taaagtgtct gattcgatgt caaacctctt gtcaactgaa
    17761 tgctgtgcga tgttatggat gttcctgtag aagataaaga agcatatggc tgagctgtta
    17821 tattgcatcc tggtttttga cagataactg atattgtctt tgttattaca tctgattctc
    17881 cttctatggc cgctataccg gataactgtg gaactgttac agttattgtc gcagaaccat
    17941 cagatacata tgtcaggact ccagatgaac ttaccataac tacgctttca tctgaagaac
    18001 tatatacaac aggtccctct ccatccagta aagatggact cagtgatggc acaattatac
    18061 gaggcgtacc ttcaagcaat aaaacagatg aaaaattcaa agacatagtt cttggtgcaa
    18121 ttatgccaag attaactcca gttatctggt caatagtagg ataacccaat gtaaacgtat
    18181 attcaccctg actgttaaat agagatgaat cagctccagg ataatattca ttgttatcct
    18241 ttataacttt tagagtatat gtaccaggcg aaataatacc tacagaatag ttaccggtgg
    18301 tatagtttga gaaattagta gaaataagtg tcccttctgc tgataaacct tcatataggt
    18361 agacataata atttctactt atctcattat catctttagt tccatttgca ttgaaatctt
    18421 tatatatagt accgctgatg tatgtatagg ctgcttgtaa tctggttggc tcagttcgtg
    18481 aaacatgaac agtatcacct gttgaagtca gagtataatt catctgtata tcaaaaaatg
    18541 cataatctga tgaacttgtc tgattatcaa agcctgatat taaaaatgga acttcaacta
    18601 tagcgctatc actgctcggg aaacttcttc cgttttcaat ctctattctt atatctgtta
    18661 cctgattcca taaacccatt acatcagaaa cataatcttc acttccctca atcttgtaag
    18721 ttacaacact gtttgtgaaa acatttccct ctagcgagac aggacctgtc aaatcagcat
    18781 tgaattttga agtgtagtta acactattgt acgtaattgt agaattttgc tttggcactt
    18841 tgatatccag cactattccc gaagcatcat ttcctgactg taatccgtta tacatatagt
    18901 atttgtatga tcctgaagaa ttatacctgt attgcatagt tgtattctcg ccttctgtca
    18961 cattggattt taagtaactt tgcgctgtca tggcgcttac agcattcaat gtaacagtag
    19021 ttctttcaga atcaatagaa taagctaact ggtcagtggt atcaccatca ttatcataat
    19081 catgaatatc aataaacata taacttgtcc tgtaaggata atatgttcca tcatgctgaa
    19141 aatatgaagc aagcggcagt ttaaatgtct gtacagttga tgttggaaca gcaggtccta
    19201 ctttgaacgt aaaagtatgt atagcatttc cattcctttt aatattatcg ttatatttaa
    19261 ctccatacaa agttgtaccg tcaccaagat ctcttattga caattcataa tctataccct
    19321 gataagcagc atttggagta tatcccatga aaatatatgt tgaaggcata atcgcataat
    19381 attcaggatt caaaacttca gcacttgtag aagcaaattg aaaattcagt gcaaaagtat
    19441 ctcctttatt aatagtggtt cgattcaatg cctgccatgc atttatatta tatgaagatc
    19501 ttaaataaaa attatgctgt agaatattct gtaacggtat cacccctaaa gtcccacttt
    19561 gtgctgatat aatctgtgat gttacctgag ttgatgtact caatgcttct tcgttagtca
    19621 tatgtgcata gaagagagtc cgacttgctg tactatgtgg aactttgtca taaaccactt
    19681 ccatccatac tatagaatca gtattatctg ataactgtaa ttgtggtaca tcttcattta
    19741 ggacagcttc atatacagtg tctcctcctg taaatgtttt gtacttcaca gttgcagtag
    19801 acggctgagt acttgttaga gatgatgaaa gattaaattc aattttatca atatataggg
    19861 tttggggata tgtatattcc attttaacat cagttataag atattctgtt ttattatata
    19921 tgcgcgatat atactttgta tttccctcta taggaagaat atagtatcca taatatgaat
    19981 atgtataaga attctgtgca taaaagaaat ctgaaatatt atattctttt atttcacagc
    20041 caagcaaatt actggagtag acagaatagc cttgaggata gcttacatga tctaaaagca
    20101 caatatctgc accatctaca ttagctttta cctgaggacg tatatttttg gaatagttac
    20161 ctggctcagt tactgttaac tttaaataaa tgttgtgatc aaatgtataa gaagtgttcc
    20221 ctgaagtaaa agtatgtgta gaaggcaatg tatataaaag agcttttcca tttccaacta
    20281 ttgcacctgc tgaatttata taattatatg attcataata tataactgaa tatgctgttg
    20341 ttccacttcc aatggtataa gagacacctt cttcaaaagc agcaaaacta cttccgcttc
    20401 ccaaacctgg aactgattcg tcaggaagtg gaacagtgta ttccacatag tcatatgagt
    20461 agtgatttgc tcttgatgga taataataga tataccctct gcacgaaaca taatactcgc
    20521 tgacttcgtc attaaaaata agtgaattct tactgtttga catactcata atatttggct
    20581 tcaaagttgg attgttagtt atagtcactg ttttactttg aatgctaatt attgtttcac
    20641 catcataata actggcactg acagtataag tcgtctcatc gagcttatat gtaggcatta
    20701 gtgttatgtt aaacccaatc gcggtcatat cagaattaaa tctgtatgtt aatacagcat
    20761 taccattttc atcctgttga ttggttactg ctcctgatgt tatcagtgtt ggagaaagag
    20821 tagcatttga tgcagtcgga taatatgaga tattaatacc ttttgggaca tttattacaa
    20881 gaatcggatt agtcatatcc actgatgtta tatcaaaagt tacattatat gtatcagaat
    20941 tattaactgt aaatgactca gaagaattag gagaaacaaa ggactgctcc atgcttaaac
    21001 tccctgtaga agcttctaca ttttgagtag gtataataag caatatagct ataatcgata
    21061 tcaatactgc cattattttc ctataattaa aatacaaaaa ggcatcaata agatgtcttt
    21121 tcaaacaaga actacgaact ttattataca ttaatctgcc ccctaattgc atttattaag
    21181 aataaatgca ttatatatat aatatataat ttttaatttt tcgtgtcaag gaatatacca
    21241 aaaatactct acattttagt ttatcgactc ttaaaatcta tttaatcttt tttagcaatt
    21301 gcctgcgtaa acgaatttaa gatttttttg ggctactttt acaagcctat ataccaaatc
    21361 aaatgatgta g
//
LOCUS       DAOSXM010000030        21271 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2111
            NODE_1573_length_21271_cov_9.035209, whole genome shotgun sequence.
ACCESSION   DAOSXM010000030 DAOSXM010000000
VERSION     DAOSXM010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864178
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 21271)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21271)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:24:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,261
            CDSs (total)                      :: 2,229
            Genes (coding)                    :: 2,218
            CDSs (with protein)               :: 2,218
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 3 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21271
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1573_length_21271_cov_9.035209"
                     /isolate="AMR_MDS_2111"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>3009
                     /locus_tag="PLQ68_05585"
     CDS             <1..>3009
                     /locus_tag="PLQ68_05585"
                     /inference="COORDINATES: protein motif:HMM:NF039533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HXK72155.1"
                     /translation="YDNIAPTVEITSTTSMDTNIYEIPVKIMFSEDVTGFALEDIVMS
                     NATPKADSFEVVSAREYNVIADAQTSGQLVTVDVAAGVAFDSAGNGNTEATQFTRRYD
                     RIRPFSVVTSPDTVYITDESPIPIVITFYQSTTANETESVTGFDLADIIVINGTAGNL
                     TGSGHIYYADITPDGEGIVDISIIDSAAQDAAGNPSRNHSSYPSLTRVYDVNPPSVNI
                     SSSVSTTNTSPIPVTFTFSEAVTGFTVDDISVSNGSLSNFIPVNGSTYTVIVTPLGQG
                     QVTVDVAAGVATDAAGNFNTAAQFTSTYDTTAPSITISSSAGSATNISPIPVTFTFNE
                     SVTGFTLADISVTNGTASNLNTTDNMTFTATIAPSSQGNVRVNVAAGVVSDTAGNGNT
                     AAAEFVCAYDTTRPSASILSSAAAYITSTFNVNIQFSEVVTGFALDDITVANGTASNI
                     TTADNRNYSVTITPTNQGTVTVNVSTNAAYDAAGNGNTLVNAMTRVYDTIGPNVTIDS
                     TAGSYTNISLIPVSITFNENVTDMQISDITVTNGTAGMLRGSGSTYTISIAPIGEDQI
                     TISIGSGSVIDAAGNANYESNEYTLIYDITSPILSISSSSNNVTNTSPIPVTFTFNEA
                     VTGFTLADITVTNGTASNLNNTDNMTFTATITPSSQGNVRVNVASSVASDAAGNGNTA
                     AAEFVCAYDTTRPSASIFSSAAAYVNSSFTANIQFSEVVTGFALDDITVTNGTASNLT
                     TADNRNYSVTITPTNQGTVAVNIATNTVFDAAGNGNSLVMGMTRVYDTVNPTVTIDSS
                     LSSYTNTSPIPLTITFSETVTGFTADDIAVTNGTKGALSGSGSTYTIDIIPFAQGAVR
                     VNILSAVATDLAGNSNTAALEFVIGYDTVAPVTTIDTMQLNKDTNDSTDFITKYADQT
                     LYIVLTHRLSLTELVEISMDNGVTWTNFNHAANSYSSNNNITLLNGTHNIKLRIVDLA
                     GNIGPVTTQQYTLDTDRPTVQVTSTVTEPT"
     assembly_gap    3010..3109
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3110..>4783
                     /locus_tag="PLQ68_05590"
     CDS             <3110..>4783
                     /locus_tag="PLQ68_05590"
                     /inference="COORDINATES: protein motif:HMM:NF040192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HXK72156.1"
                     /translation="LDTDRPTVQVTSTVTEPTNQSPIPITITFSEDVIGMQLSDLLIE
                     NGTASSLLGSGNIYTAQITPSGNGIVRLRLGYGSVMDAAANAIDASSYYTWTYDGTSP
                     TVEITSTETTFTNTSPIPITITFSEAVTGLTASDITVANGAVSNFNTADNITYTADII
                     PSAQGIVTVDVAAGVSADATGNVNTAATTLTRTYDSITPTVVISSATSSSTNVTPIPV
                     TITFSENVTGFTINDITVINGVAGGFVSLSGSVYTALITPAGNGEVIVDVLADTASDL
                     GGNSNSAALSLTRIFDSIRPTVIISSSVSDYTINSLVALTITFSKNITGFDINDIVIS
                     NGNASNFTPINNTTYTVDIIAVNQGNITVNIPDAIAQDTAGNSNTAATELAYIYDNQT
                     PVVNSTTEISVDVTDTESIRFSWDAATDNISSASNLLYKAYYSIRDDIGNLTDILSNG
                     IAINDWSDDMSVTATGLSASTIYYINVIVKDETGNMSAYSKVTGITDDEEEVDEDIPL
                     PNNTVITVDGQEQEAANIKRETTQGVTRITVTVDDFLVSNLMQSEEEGTV"
     assembly_gap    4784..4883
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4884..5537
                     /locus_tag="PLQ68_05595"
     CDS             <4884..5537
                     /locus_tag="PLQ68_05595"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HXK72157.1"
                     /translation="TIIREGNKYYAVINSLTNSTYTVIYNNIEIKDAKGHWAQTELND
                     MASRLVIQADFEGKFNPDEKITRAQFSRFIVKALGLVPDSYENIFTDVSSELEDSVYI
                     EKAYEYGIINGYGNGLFGPTDLLTREQATTMLERAMKLAGLDVDMKNEELDLILDKFT
                     DKNKISSYAINSVALCVRNGIVIGRLNDDIDPGANLTRAEAVVMLRRMLQEADLINK"
     gene            5732..>6633
                     /locus_tag="PLQ68_05600"
     CDS             5732..>6633
                     /locus_tag="PLQ68_05600"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HXK72158.1"
                     /translation="MNYDICIIGAGVVGSLIARQLSKYNVKIAVIERNVDVSMDATGA
                     NSGIVHSGYDAKNGSKKAILNVEGCKMMPKLCEDLSIKCKMIGSFVLAFDKEDELVLQ
                     QLYDNGIENGVTDMSIVERDFILQKEPYVNPSVTKALYAQNAGIVSPYKLNIAAMDNA
                     ISNGVTFLRETKVTGIEKKDGLFNISVDVTGDICSSSKVDPIKEENRTDITAKIVINA
                     AGAFADEISKMAGDDSFKIIPRRGEYILFDKTEGSKINTVLFQTPTDKGKGILVTPTV
                     DGNLMIGPDSTVASEKDDKCTNSE"
     assembly_gap    6634..6733
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            6798..8075
                     /locus_tag="PLQ68_05605"
     CDS             6798..8075
                     /locus_tag="PLQ68_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009256283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HXK72159.1"
                     /translation="MKNFNLVIIGGGPAGMAAALEAYDLGIKDIAILERDDSLGGILK
                     QCIHNGFGLHVFKEELTGPEYALRFSEKVKDTDITVMLNTMVIGADNNKTMYAVNPEY
                     GFMTIKAKAVILAMGCRERPRGALRIPGANVAGIFTAGTAQRFVNMEGILPGSKAVIL
                     GSGDIGLIMARRLTLEGIKVQGVYEIMDYPGGLTRNIVQCLDDFNIPLHLSTTIVKIH
                     GKGRVTGVTTAKVDKNMLPIKGTEEYVECDTLLLSVGLIPENELTEQMGVGMYQVTGG
                     AYVDQDLETDTEGIFACGNVLHVHDLVDYVTMEGQKAAKSAKKYLDDLLEQKKTKPEI
                     KVIPGNGLRYVLPQHIYLKDPEEKIKVFFRSKARYKDVVIKAIMNDEVIAKKKVIRLI
                     PSEMESVEIKTSDLFRTTNNGADLKDLVIEVEQ"
     gene            8072..8443
                     /locus_tag="PLQ68_05610"
     CDS             8072..8443
                     /locus_tag="PLQ68_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017211150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1667 domain-containing protein"
                     /protein_id="HXK72160.1"
                     /translation="MKKDITCIVCPKGCTIHVEYDETGIKSMEGYSCKRGIEYATAEI
                     LSPTRMVTSSVKTDNARYTVCPVKTSKPIGKDKIQDVMKSICCVKVKAPVRQGDVIIK
                     DVCGTGADIVATQTLEALEKE"
     gene            8440..9843
                     /gene="murC"
                     /locus_tag="PLQ68_05615"
     CDS             8440..9843
                     /gene="murC"
                     /locus_tag="PLQ68_05615"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HXK72161.1"
                     /translation="MKAEFLKGINHVHFIGINGSSMSGLAILLFEKGIKVTGSDIEHT
                     HTMDKLISMGIEVYVPHDSKKMGKPDLVVYTAAVHSDNAEYIFAEKNNIPLMDRALLL
                     GKIMDEFKYSIAISGAHGKTTTTTLTAYLLEQMGFDATIHDGGEAPFLNGNVKSGNSD
                     YFITEACEFNGSFLKLNPYISVILNIDFDHVDWFKTFDNMKKSFLEFANRTDKDGYLV
                     ACYDDVPTREVASQVKCNVVYYGLNNTALDYTALDIVIETDGKASYDLYVKDVKVCRV
                     SLGAEGIHNVLNSLAVIAVLDLLGADIHKASSHLSYIHGAKRRFDLQGEADGVKVYSD
                     YAHHPTEIAVTLETASKMKHRKLYAAFEAHTFSRVRVLFNEFAKCFAKADTAIIADIY
                     NDRESADFEVSGMKLAEAILKNGTDSIYLPSYKAIEDYLYEHVQPGDVVMVLGSKYLE
                     TICMPLVERLKDRESGN"
     gene            9921..10517
                     /locus_tag="PLQ68_05620"
     CDS             9921..10517
                     /locus_tag="PLQ68_05620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72162.1"
                     /translation="MRKKKKAKEAALLSLYRRNSQTGNIIIDVALDRYLEFFHEWDNA
                     VFKKRDLNPELAEFLDICSEDIPLSKQFDVHFHIKNIALDSEKEKLIIASYHNYYTAL
                     KKLEDRKVKRIARFSVILFVIALGFIFLNLIANARAGNQLLPKVLLEGLLIGGWVFMW
                     EALHMLFFDILEPFKRKRELKRFLNAEITFSSEIDKGV"
     gene            10521..11525
                     /locus_tag="PLQ68_05625"
     CDS             10521..11525
                     /locus_tag="PLQ68_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012492118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HXK72163.1"
                     /translation="MDNRDWFKKAGYGMMVHWGLYSLLAGEYKGLKSGHYAEWIQSYH
                     KIPIKEYEKLCQAFNPVFFDADEWVRFAKECGMKYFVVTSKHHDGFALFKSKADKYNV
                     CDMTPFKRDIVGELAESAYKYGLKFGLYYSQDIDWHEMHGGGYDTERISAGTTWDNIW
                     DYPDKTIKNYDICFENKIKPQVEEILKNYGELLLIWFDVPVTLKERHSKQLYDMVKKY
                     QPDCLINSRLGNGIYDYVSLGDNEIPDSLDNIRGAKKSPFGLYESAATLNDTWGFSYH
                     DHNWKRPEQIAAYRKQLNDLGINYLINVGPDGLGRIPSPSIDILKGAYELFPLIQEEF
                     "
     gene            11569..12264
                     /locus_tag="PLQ68_05630"
     CDS             11569..12264
                     /locus_tag="PLQ68_05630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001006716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate polymerase domain-containing
                     protein"
                     /protein_id="HXK72164.1"
                     /translation="MKQVLRQEKKYLISKSDYIKYSAAFNTYLAQDENNGEHGYTVRS
                     LYFDTLFDNDFNEKEEGVEIRRKIRLRIYKPTDEFASLEMKQKQGSQQLKRSLKMSKE
                     DAILLTKGVYGPLLNYKESFAKEIYGVMNMRFYRPKAIVEYNRKAFIAKENSIRITFD
                     HNIRATESCFDIFSDKLNLYPVFSADGGVLEVKYNGFLLSYIKDMLDRIEKSELSVSK
                     YALSRTIGLNYVF"
     gene            12276..12953
                     /locus_tag="PLQ68_05635"
     CDS             12276..12953
                     /locus_tag="PLQ68_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006060633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HXK72165.1"
                     /translation="MTQYLLKLLEQGKTLTLQDILSHLVVAVAIGIVIYLSYMYTHSG
                     TIYSRKFNVSLLMLTLLTTMVMTVIGNNIALSLGMVGALSIVRYRTAIKDSRDTAYIF
                     WAIIVGICCGAGDYLVAAIGSAIVFILLLVFGKIKNDNRMLVIIKGARGLERQIEAIV
                     FNYYFAKANLRVKNTTDLTVEYIYEVSKKIMRKAAVNEKSITDKLYELQHIEYVNIVE
                     QNDEVNN"
     gene            12965..14377
                     /locus_tag="PLQ68_05640"
     CDS             12965..14377
                     /locus_tag="PLQ68_05640"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HXK72166.1"
                     /translation="MRRKITSIIAIICLVAISIGFTFLNSDNIRYHQHLMAQSHQNCS
                     HSTDDFCTHIPLIKIDTSSQQIPAFEDGKIFIESDISIFDKEDYNNHLYDQPDIQSTI
                     LIRARGNSSLFFDKKSYFIKLINDDHSENKQKVLGMDNHYEWVLHGPILDKTLIRNYI
                     SMNLSGQIMKTAPDVRFCEVFINDQYMGLYVMMESISRAEGRVDIAKYNENDPFTSYI
                     IRLDRGSDPEEEITLFSGYTYNSDSIANIVYPPKHYLNEQLNKYISDNISQFEKALYS
                     YDYDDPKLGYRKHIDVMSFVDYLIINEFTQNYDAGIYSTYLFRDIKGKLEIGPVWDFN
                     NAYDNYMETPINTTGFSFQNKVWYIMLMKDEYFVNLVIQRYKQLRKTVLSEEYVIGYI
                     EDTVEYLGPAIDRNFEVWGYTFLADRGMLLPKDRNHKSYEAALNQLKQFIIDRGNWMD
                     DNIDSLMQFCHDSRNKSFND"
     gene            14386..16557
                     /locus_tag="PLQ68_05645"
     CDS             14386..16557
                     /locus_tag="PLQ68_05645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72167.1"
                     /translation="MKKFLIGTVTIIVLALSINYILFYTSFYIDFNPKATITSSFKAE
                     NESIYILKDGEYERFEIRGVNIGNTVPGKFGTDYAIDKYDYLRWFKQIQEMGANTISV
                     YQIMNADFYNALYQYNKDNDDPLYLIHGLAISDYSQNSHIDATDKDFYEDIIRQIYIT
                     VDILHGSRQVILNQASGLGFYLRDVSQWVMGYILGSGWNSYTIAYTNELHKDHYGYSG
                     KYMYTSEDATAFESVLAMLGDTLIKYETERYKEQRLVSFFNWPSTDPFEYPDYITEFF
                     KKCAEVDVENIKTTNQFMSGQFASYHAYPYYPGYLNYYENKEFWFADAQGDVNTYTAY
                     LKMLMLHHTMPVVITEYGVPTARGMAHEDTNKGYNQGRNSEFEQGQIILDTYKQIKSV
                     DCAGSLLFVWQDDWTRTTWNTQHSVDEKRSPYWSDAQSSDQGFGLLAFDPGKKEVLCH
                     VDGNIDEWLDILPVAKTKDMSLFMQYDEKYIYFLVHKENYNHINDVLYIPIDTTDKSG
                     SNYSVDHDVRFDSNADFLMVINGKEESRILVQERYDTLRAMYNQYIEGVNPYTDPTNV
                     NSPKFNKIQLILQLEAVEKINNPELMASTYETGHLTYGNSNPHSQDYNSLADFYIEGD
                     YIEIRIPWQLLNFSDPSKMRIHDDYYLHYGVEFTTINKLLVGIGSVDYKGHIIHMNEF
                     LLKKWDESVAYHERLKQSYYMIKEAWTNEILKKEVQGAVDE"
     gene            16550..17665
                     /locus_tag="PLQ68_05650"
     CDS             16550..17665
                     /locus_tag="PLQ68_05650"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HXK72168.1"
                     /translation="MSDLRIDIVLIIYIIICVLMILFNTAFLIYLRYKDYIFRNKRKR
                     LENIIKSQINRISKNEVIDPKIQEFLFKKLISIRYLKSFHEIIKNESKKNETDTFRYL
                     KKCKPIFEYLIVEFSNKENAKKAYLAFLISEFKLCDNLHYDFIVSSMISYTMNKSIYV
                     RQNALNALYMSNNSEAILSAIMRMQELKISHSNKLISDGLLLFNGDRAELASLLWKEF
                     DKFDLNVRIAIVNFIKTASLDYAEEFYQRLTNKYTHKEVKLSLVRYFGKMIYQPAGDY
                     LITMMKQDTPEWEYKAIAATALAKYKSQQVIDVLKEALKSDNWYIRFNASESLVAQNI
                     GYMELIDIYNGTDRYAREILEYKMETSRLKKQAKEGE"
     gene            17666..19069
                     /locus_tag="PLQ68_05655"
     CDS             17666..19069
                     /locus_tag="PLQ68_05655"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016285649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXK72169.1"
                     /translation="MFIEALRIVMLIVGVFYSLYLLVYATFLVVSVTVGASELYKNQQ
                     MSRLHNEISHEYYFPVSIVVPGYNEEITICDTVNSLLNLEYKLYEIVIVDDGSKDNTS
                     KVLIDTFKMKRVRRKIKKSIRCQEEEFVYESYGYKVPIVLIRKKNGGKADALNMGINA
                     SRYPYFICMDADSMLQHDALERIVKPVLTDENLIACGGLVRIANGVVLENGRVKKYGM
                     PKKMVTAMQVLEYERSFLASRILFDMFNGNMIISGAFGLFKKDIVIAVGGYDHGTMGE
                     DMELVVRLHVFCKKNNIPYTIKYEPEAVCWSQAPESMSDLKKQRRRWHLGLFQSLSKH
                     SQVLLSTKYGALGTVSYMYFVIFELMSPYIEIFGLFSVIIMYFLGMISLRVIIMFSVL
                     SILFGAVLTLSAFFSRIHTQNVKLSSKDVWKAIWACIYESVWFRVILAFTRMTAFIGY
                     KKKKLQWGKIKRVKLNQ"
     gene            19105..19557
                     /locus_tag="PLQ68_05660"
     CDS             19105..19557
                     /locus_tag="PLQ68_05660"
                     /inference="COORDINATES: protein motif:HMM:NF016959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HXK72170.1"
                     /translation="MISFRGIKKELFGYSKKDVMNYIEMLNEDLNTKLSDKDRQVKEV
                     IEQNNILLKRLEKLEKTQDEIAYAIIQAKKSADLIVNDANKQAEKILKDTKENKQKQE
                     SEYNEYIEQIIKFKKQMTRILKSFVKEAENIKKECDEACRVSFGREHE"
     gene            19550..>21271
                     /locus_tag="PLQ68_05665"
     CDS             19550..>21271
                     /locus_tag="PLQ68_05665"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HXK72171.1"
                     /translation="MNRRAIIISVIIILLLSVHTFSGIALGEDFVMSQIDKETVQNYP
                     VIYVAIEKASFPFEFYNNYEKQYEGVIVQILKKVSYLTGFKFEYIYTKNSNETLQFAY
                     NMQTDITTSFVRPYSGFKAGREIASFDGNIIAIQYTSRMPNQAIELMNRAIDTISQEQ
                     IVSIISSQAVQYEKMFNDTKLNIVIATSVAVVSITALVSAFTAYKKCKKLKQQFLNTD
                     KLTGYPNLTGLKSITDITISSLNISSYCMVNFVVSSLDNIREIFGYDEANKVQVDVAL
                     IIDEYIYNNEILARADNDDFILLLQFTEKPEMALRIKQIIDAINDKFDIKKSEYPVVL
                     SIGIYTPKYEEVNMDMLMQKSLQARRFVKETDEIRFLFFDETAHIVTIEENLLQQEFT
                     TSMEKEDFLIYIQPKISLRDNSICGGEILSRWYNPRRGLIRPGEYIPVFEKSGSIIAF
                     DMYMFEKACKLIAKSMKEGKRVVPVAINFARSHFADIKFCDDILDIAQRYEVPASLLN
                     IEVTESIISENKKIMIEIVKRLKEYGFTISLDDFGTGYASFSDLSSFTIDYLKLDKSL
                     LYNLDNSR"
BASE COUNT         7463 a         3211 c         4206 g         6091 t
ORIGIN      
        1 aatacgataa tatagctcct actgttgaaa ttacttcaac taccagtatg gatacgaata
       61 tatatgagat acctgtaaaa attatgtttt ctgaggatgt taccggattt gcattagagg
      121 atatagtcat gtctaatgcg acacctaaag cagacagttt cgaagttgtt agtgcaagag
      181 aatataatgt tattgccgat gctcaaactt caggacaact tgtaacggtt gatgttgcag
      241 caggagttgc ttttgattct gctggaaatg gaaatactga agcaactcag tttaccagaa
      301 ggtatgacag aattcgacct ttttcagtag tcacatcacc tgacacagta tatataactg
      361 atgagtcacc aatacctata gtaattactt tttatcagag tacgacagcg aatgagacag
      421 agagtgtgac agggtttgat ttagcagata taattgttat taatggtact gcaggtaatt
      481 taactggaag cggccatata tattatgctg atattactcc agatggggaa ggtattgttg
      541 atatcagtat aatagatagc gcagcacagg atgcagctgg aaatcctagc agaaatcaca
      601 gttcatatcc gagtcttacg agggtttatg atgtaaatcc tccttctgta aatatatctt
      661 catcagtctc tactacaaat acatcaccta taccggtaac attcacattc agtgaagcag
      721 taactgggtt tacagttgat gatatatcag tatcaaacgg aagtttaagt aattttattc
      781 cagttaatgg ttccacatat acagtgattg ttacaccttt aggacaagga caggtaacag
      841 ttgatgtagc agcaggagtc gcaacagatg ctgcaggcaa ttttaatact gctgctcagt
      901 ttacaagtac atacgacacg acagctcctt ctataacaat atcctcatca gcaggctctg
      961 caacaaatat atcacctata ccggttactt ttacattcaa tgaatcggtt acaggtttta
     1021 cgttagctga catttcagtt acaaatggta cagcaagcaa cttgaacacc actgataata
     1081 tgacattcac agcaactata gccccatcat cgcagggtaa tgtaagagtt aatgttgctg
     1141 caggtgttgt ttctgatact gcgggtaatg ggaatacagc tgcagccgag tttgtgtgcg
     1201 catatgatac aacaagacct agtgcttcta tattatcttc agctgctgca tacattacat
     1261 caacatttaa tgttaatata cagttttcag aagttgttac tggatttgca ctagatgata
     1321 taactgttgc aaatggtaca gcaagcaata taactacagc agataaccga aattattcag
     1381 ttacaattac tccgacaaat cagggtacag taacagtaaa cgtttcaaca aatgcagctt
     1441 atgatgcagc aggcaatgga aatactttag ttaatgcaat gacaagagta tatgacacta
     1501 ttggcccgaa tgttacgatt gattcaacag ctggttctta cacaaatata tcgcttatac
     1561 ctgtttccat tacattcaac gagaatgtta ctgatatgca gatatctgat attactgtta
     1621 caaatggtac agctggtatg ttaagaggaa gtggaagcac atatacaata tcaatagcac
     1681 ctataggaga agatcaaatt acaataagca taggaagtgg tagcgttata gatgctgcgg
     1741 gtaatgcaaa ttatgaatcc aatgaatata ctctgattta tgatataact tcaccaattc
     1801 tttcaatttc atcaagttct aataatgtaa cgaacacatc acctatacca gttactttta
     1861 cattcaatga agcagttaca ggttttacat tagctgacat tacggtcaca aacggtacag
     1921 caagcaactt gaataatact gataatatga catttacagc aactataacc ccatcatcac
     1981 aaggaaatgt aagagttaat gttgcttcga gtgtcgcttc tgatgctgcg ggtaatggga
     2041 atacagctgc agccgagttt gtatgcgcat atgatacaac aagacctagt gcttctatat
     2101 tttcttcagc tgctgcatat gttaattcat catttactgc aaatatacag ttttcagaag
     2161 ttgttactgg atttgcattg gatgatataa ctgttacaaa tggtacagca agcaatttaa
     2221 ctacagcaga taaccgaaat tattcagtta ctataactcc aacaaaccaa ggtactgtgg
     2281 cagtaaatat tgctacgaat acagtttttg atgctgctgg aaatggtaat tcattagtta
     2341 tgggtatgac aagagtatat gatacagtga accctactgt aactattgat tcatcattaa
     2401 gttcctatac aaatacatca cctatacctt taacaattac atttagtgag acagttacag
     2461 gctttacagc agatgatata gcggtcacaa atggaactaa aggtgcgtta agtggaagtg
     2521 gaagtacata tacaatagat ataataccat ttgcacaagg tgctgtaaga gttaatatat
     2581 tatcagcagt cgcaacagac cttgctggta atagcaatac tgcagctttg gaattcgtaa
     2641 taggttatga tacagttgct ccggtaacta caatagatac aatgcaactt aataaggata
     2701 ccaatgatag tacagatttt attactaaat atgctgatca gaccttgtac attgtactaa
     2761 ctcacagact ttcattaaca gagctcgtag agatatccat ggataatgga gtgacatgga
     2821 ctaattttaa tcatgcagca aactcatatt ccagtaataa caatattacg cttcttaatg
     2881 gtacccataa tattaaatta agaatagttg atcttgcagg aaatataggt cctgttacta
     2941 cacagcagta tacccttgac actgatagac ctacagttca ggtaacatcg acagtgaccg
     3001 aaccgactan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc ttgacactga
     3121 tagacctaca gttcaggtaa catcgacagt gaccgaaccg actaatcagt cacctatacc
     3181 gataacaatt acatttagcg aagatgttat aggcatgcag ttatcagact tgcttattga
     3241 aaatggaact gccagttcat tgcttggcag cggtaacata tatactgctc agataacacc
     3301 atcaggaaac ggtatagtca ggttgagatt agggtatggt agtgttatgg atgcagcagc
     3361 caatgctatt gatgcgtcca gttactatac atggacatat gacgggactt cgcctacagt
     3421 agaaattact tcaacagaaa cgacctttac aaatacatca ccaataccta taactattac
     3481 ttttagcgag gctgttacag gattaacagc atctgacata actgttgcaa atggagcagt
     3541 aagtaatttc aatactgccg acaacattac atatacagct gacatcattc cttcagcaca
     3601 aggaatagtt acagttgatg tagctgcagg agtatcagct gatgctactg gtaatgtaaa
     3661 cactgcagca acaacgttaa caaggacata tgactcaata accccgacag tagttatcag
     3721 ctctgcaaca agcagttcga cgaatgtaac acctatacca gtaactatca ctttcagtga
     3781 gaatgttacc gggttcacaa tcaatgacat tactgtgata aatggtgttg caggaggatt
     3841 tgtttcgctc agcggatcag tgtatacagc tcttattaca cctgcaggaa atggagaggt
     3901 tattgttgat gtcttagcag acacagcttc agatttaggg ggaaattcta acagtgcggc
     3961 tttaagtttg accagaatat tcgacagcat caggccgact gtgattatat cttcttcagt
     4021 atctgattat acgattaatt cattggttgc tttgacaata actttcagta agaatatcac
     4081 tggttttgat ataaacgata tagtaataag caatggaaat gcaagtaact ttacaccaat
     4141 taacaacaca acatataccg ttgatatcat agctgtaaat caaggaaaca taactgtgaa
     4201 tattcctgat gcaatagccc aggatacagc aggtaatagc aatactgcag cgacagagct
     4261 tgcttatatc tatgacaatc aaactccagt agtaaattca acaacagaaa tatcagttga
     4321 tgtaacagat acagaatcaa ttcgtttcag ctgggatgca gcaactgata atatatcatc
     4381 agcatcaaac ctgttataca aagcgtatta ctcaataaga gatgatatag gaaatttgac
     4441 ggatattcta agtaacggca tagctataaa tgactggtca gatgatatgt ctgttacagc
     4501 tacaggctta tcagcttcaa caatatacta cattaatgtt attgttaaag atgaaactgg
     4561 aaatatgtca gcatatagca aggttactgg cattactgat gatgaagagg aagttgatga
     4621 agatattcct ttgccgaata atacagtaat tactgtggat ggacaagagc aggaagctgc
     4681 taatattaaa agagaaacta ctcaaggtgt aaccagaata actgtaacag tagatgattt
     4741 tcttgtaagc aatctgatgc agtcagaaga ggaaggtaca gtannnnnnn nnnnnnnnnn
     4801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4861 nnnnnnnnnn nnnnnnnnnn nnnacaataa taagagaagg taataagtat tatgctgtaa
     4921 taaacagcct gacaaatagt acatataccg taatatataa taatattgaa attaaagatg
     4981 ctaaaggcca ctgggctcag acagaactta atgatatggc atcgagactt gttatacagg
     5041 cagattttga aggcaaattc aatcctgatg aaaagataac ccgtgctcag ttctcaaggt
     5101 ttatcgttaa agctctgggt cttgttcctg attcatatga aaacatattt actgatgttt
     5161 caagcgaact ggaagattct gtatatattg aaaaagctta tgaatacggg ataatcaacg
     5221 gatatggcaa tggattattt ggaccgactg atcttcttac cagagaacag gcgactacta
     5281 tgcttgaaag agctatgaag cttgcaggtt tggatgttga tatgaagaat gaagagctgg
     5341 atttaatact tgataagttt accgacaaaa acaagatatc ttcatatgca ataaattctg
     5401 ttgcattatg tgtaagaaat ggaatagtta tcggaaggct taatgatgat atagacccag
     5461 gagcaaacct gacgcgtgca gaggctgttg ttatgttaag acgtatgcta caggaagctg
     5521 acttgataaa taagtaaaat ttatatatta tgtattgata ttgtgtctga cctcgttgca
     5581 aaaaaggcaa ctcaagtata gaattactat atatggaagg gagaacccat gaaaagagtc
     5641 attgcaatca tattagccgc ggtattggtg ctttccctat tgctggcaat ggtcctgcaa
     5701 agcggcatac tatatagcta acggagggca tatgaactac gatatatgca ttattggggc
     5761 tggagtagtc ggaagcctta tagctaggca gttatcgaaa tacaatgtaa aaatagcagt
     5821 aatagaaaga aatgttgatg tttctatgga tgctacaggc gcaaattccg gaatagtcca
     5881 ttccggatat gatgctaaaa atggttcaaa aaaagctatc ttaaatgttg aaggctgtaa
     5941 gatgatgccg aaactatgtg aagacttatc aataaaatgt aaaatgatag ggtcattcgt
     6001 acttgctttt gataaagaag atgaattagt cttgcaacag ctttatgata acggaattga
     6061 aaacggagtc actgatatgt ctattgtaga aagagacttt attttgcaaa aagaaccata
     6121 tgtaaatcct tcagttacaa aagcccttta tgcccaaaat gccggcatag tttcgccgta
     6181 taaactcaat attgccgcga tggacaatgc aataagcaat ggtgtcactt ttttaagaga
     6241 gacgaaagtt acaggtatag aaaaaaaaga cggattattt aatatatcag tagatgtaac
     6301 aggagacata tgcagcagca gtaaggtaga tcctataaaa gaagaaaaca gaactgatat
     6361 tactgcaaaa atagttataa acgcagctgg tgcatttgct gatgaaataa gtaaaatggc
     6421 aggtgatgat tcctttaaga taattccaag aagaggcgaa tatatactgt ttgataaaac
     6481 cgaaggttcg aaaattaaca cagtgctttt tcaaactcct acagataaag gaaaaggtat
     6541 attggttact cccacagtag atggtaacct tatgatagga ccagattcga cagtggctag
     6601 tgaaaaagat gataaatgca caaatagtga aaannnnnnn nnnnnnnnnn nnnnnnnnnn
     6661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6721 nnnnnnnnnn nnnataccag ttaacaagat agcaaaagcc aatgctgatt catgtgttct
     6781 tgggaggtat tcccgtgatt aaaaacttta atttagtaat aataggcgga gggcctgccg
     6841 gaatggcagc tgctctggaa gcatatgatc tgggtattaa ggatatagcg atacttgaaa
     6901 gagatgactc attagggggg attctcaaac aatgtataca taacggattc ggacttcatg
     6961 tttttaaaga agaacttact ggtcctgaat atgctttgcg attttctgaa aaggttaaag
     7021 atacagatat aactgttatg ctcaatacta tggttattgg cgctgataac aataaaacga
     7081 tgtatgctgt aaatcctgaa tacgggttta tgactattaa ggcaaaggct gtaatacttg
     7141 caatgggatg cagggaaaga ccaagaggag cattaaggat tccaggtgct aatgtcgcag
     7201 gtatattcac ggctggtact gctcaaagat ttgttaatat ggaaggtata ctgcccggtt
     7261 caaaggcagt catacttgga agcggagata tagggcttat aatggcaaga aggcttacac
     7321 ttgaaggaat taaggtccag ggagtgtatg agataatgga ttatcctggt ggtcttacca
     7381 ggaatatcgt acagtgtctt gatgacttca atataccttt gcacctttct actacaatag
     7441 taaagattca tggaaaaggc agagttaccg gggtaactac agctaaagtt gataagaata
     7501 tgttgcctat taagggaact gaagaatatg ttgagtgcga cactcttttg ttatctgtcg
     7561 gccttatacc ggaaaatgag ttgaccgaac aaatgggtgt gggtatgtat caggttacag
     7621 gcggagcata tgttgaccag gatctggaaa ctgatacaga aggcatattt gcctgcggga
     7681 atgttcttca tgtgcatgat ttagtggatt atgtaacaat ggaaggtcag aaagcagcta
     7741 aaagcgcaaa gaaatatctt gatgatttgt tagaacagaa gaaaaccaag cccgaaataa
     7801 aagttattcc aggtaatggt ttgaggtatg tactgcctca gcatatatat ttaaaagatc
     7861 cggaagaaaa gataaaagta tttttccgct caaaagctag atacaaagat gtagttataa
     7921 aagcgattat gaatgatgaa gttatagcaa agaagaaggt aataagactc ataccttcag
     7981 agatggaatc agtggaaata aaaacatcag atctgtttag aacgacaaat aacggagcag
     8041 accttaaaga tctggtaatt gaggtagaac aatgaagaaa gatataacct gcatagtatg
     8101 tccaaagggt tgcacgatac atgttgaata tgatgaaacc ggcattaaaa gcatggaagg
     8161 ttatagctgc aaaagaggta tagaatacgc aactgcagaa atcctttcac cgacaagaat
     8221 ggtgacttcc tctgttaaaa ctgataatgc aagatatact gtctgtcctg taaaaaccag
     8281 caaacctata ggaaaagata aaatacagga tgttatgaag agtatatgct gtgtgaaagt
     8341 aaaagctcca gtaaggcaag gagacgtcat aatcaaagat gtttgtggaa caggggctga
     8401 tatagtagct acccagacac tggaggcact tgaaaaagaa tgaaagcgga atttttaaaa
     8461 ggcatcaacc atgttcattt cataggcatt aacggctcat ccatgtccgg tcttgcaata
     8521 cttctgtttg aaaaaggaat aaaagttact ggttcagata tagagcacac tcatactatg
     8581 gataagctta tatccatggg tattgaagta tatgttccac atgacagcaa aaaaatgggc
     8641 aaacccgacc tggtagtgta tacagcagct gttcattcag acaatgcgga gtatatattt
     8701 gcagaaaaaa acaacatacc actaatggac agagcgcttc ttcttggtaa aataatggat
     8761 gaattcaaat acagcatagc tatttcagga gcacatggta agactacaac aacaactctt
     8821 acagcctacc ttttggagca gatgggtttt gatgctacta tacatgacgg gggcgaagct
     8881 ccttttctga acgggaatgt aaaaagcggt aattctgact acttcataac tgaagcatgt
     8941 gaattcaatg gaagttttct gaagctgaat ccatatatat cagtaatttt aaatatagat
     9001 tttgatcatg tcgactggtt taagactttt gataacatga aaaagtcctt tttggaattt
     9061 gcaaacagaa ctgataaaga cggttatctt gttgcctgtt atgatgatgt tccaacaaga
     9121 gaggttgctt cgcaggttaa gtgcaatgta gtatattacg gattgaataa tacggcattg
     9181 gattacacgg ctttagatat tgtcatagaa actgatggaa aagcttcata tgacttatat
     9241 gttaaagatg taaaagtctg ccgggtaagt cttggtgctg aaggtattca taatgtgctt
     9301 aattcattgg cagtaatagc agttttagac ctcttaggag cagatattca caaagcaagc
     9361 agtcatcttt catatatcca tggagcaaaa agaagatttg atttacaagg agaggctgat
     9421 ggagtaaaag tatactccga ttacgcacac catccgacag agatagctgt aacactggaa
     9481 acagcaagta aaatgaaaca cagaaaacta tatgctgctt tcgaagcaca tacattttca
     9541 agggtcagag tgctgtttaa tgagtttgca aaatgctttg ctaaagctga tacagctata
     9601 atcgctgaca tatataatga tagagaatca gcagattttg aagtgtcagg aatgaaactg
     9661 gcagaagcta ttctaaaaaa tggaacagat tcaatatatc ttccttcata caaagcaata
     9721 gaagactatc tttacgaaca tgtgcagcca ggtgatgttg tgatggtgct tggctcaaag
     9781 tatcttgaaa ctatatgcat gccattagtt gaaaggctta aagacagaga atctggcaat
     9841 taaagtaaag cagtattgta aatattaatt aaaggcaaca atctgttgcc ctatttatat
     9901 gtaaagaata taatttaaat atgaggaaaa aaaagaaagc taaagaagca gctcttctga
     9961 gcttatatag aagaaattcc caaacaggca acatcataat agatgtagct ttggacagat
    10021 atctggaatt tttccacgaa tgggataatg ctgtattcaa aaagagggac ttgaatcctg
    10081 aacttgcaga gtttttagac atttgctcag aagatattcc tttaagcaaa cagtttgatg
    10141 tacactttca tataaagaac atcgcactgg attctgaaaa agaaaaatta attattgcta
    10201 gttatcataa ttactatact gccttaaaaa aacttgaaga tagaaaagta aagaggatag
    10261 caagattttc tgtaatattg tttgtaatcg cattagggtt tatatttctc aaccttatag
    10321 caaacgcaag agcaggaaac cagttattac caaaagttct gctagaaggc ttattgatag
    10381 gtggctgggt atttatgtgg gaagctttgc atatgctttt tttcgacata ctggaacctt
    10441 ttaaaagaaa acgggaacta aaaagatttc tgaacgcaga aattacattt tccagcgaaa
    10501 tagacaaagg agtatgattt atggacaaca gagattggtt taaaaaagca ggatatggga
    10561 tgatggttca ttggggtctt tattctttgt tagcaggtga atacaagggg cttaaaagcg
    10621 ggcattatgc tgaatggata cagtcatatc ataagatacc gataaaggaa tacgaaaaat
    10681 tgtgccaggc attcaatccg gtattctttg atgctgacga atgggtccgc ttcgcaaaag
    10741 aatgcggtat gaaatacttt gttgttacct ccaagcatca tgatgggttt gctcttttta
    10801 aatctaaagc ggataaatac aatgtctgtg atatgacgcc atttaaaagg gatatagttg
    10861 gagaattagc agaaagcgca tataagtacg gtcttaaatt cggactgtat tattctcaag
    10921 atatagactg gcatgaaatg catggtggag gttatgacac tgaaagaatt tctgcaggta
    10981 caacatggga caatatctgg gattatcctg acaaaacaat taaaaattat gatatatgct
    11041 ttgagaataa gataaagccc caggtagagg agatactgaa aaattatgga gagcttctgc
    11101 ttatatggtt tgatgtacct gtaacactca aggaaagaca cagtaagcaa ttgtatgaca
    11161 tggttaagaa atatcagccg gattgtctta taaactcacg tcttggaaat gggatatatg
    11221 attacgtttc tttaggggat aacgagatac ccgatagttt agataatatc cggggagcta
    11281 aaaaatctcc tttcggatta tatgagtcag ccgctacact gaatgatact tggggctttt
    11341 catatcacga tcataactgg aaaagaccgg aacagattgc cgcatacaga aagcaattga
    11401 atgatttagg cataaactat cttataaacg taggacctga cggactggga agaataccaa
    11461 gtccatcaat agatatattg aagggagcat acgaactgtt tccgcttata caagaggaat
    11521 tctgaagaca tattcataga catgtaggtt tgtagtatag gggtaaacat gaagcaggta
    11581 cttagacagg aaaaaaaata tctcatatca aaaagtgatt acataaagta ctcggctgct
    11641 ttcaatacat atcttgcaca agatgaaaat aatggtgaac atggatatac tgtaaggtct
    11701 ttgtattttg acaccttgtt tgacaatgac tttaatgaaa aagaagaagg agtagaaata
    11761 agaagaaaaa ttcgccttcg gatttataaa ccgactgatg aattcgcatc acttgagatg
    11821 aaacagaagc aaggttctca gcaactaaaa agatctttaa agatgagtaa agaagatgct
    11881 attcttctta ccaagggagt gtacggtcct ttacttaatt acaaggaatc ctttgccaaa
    11941 gagatatatg gcgtgatgaa tatgcgtttt tacagaccta aagcaatagt ggaatacaac
    12001 aggaaagctt ttatagctaa agagaacagt ataaggataa catttgacca taatataagg
    12061 gcgacagaaa gctgctttga cattttttct gataagctga atttgtatcc ggtatttagt
    12121 gcagatggag gagtactgga agtaaaatac aacggatttc tgctttcata cattaaggat
    12181 atgctggaca ggattgaaaa atctgaatta tctgtaagca aatatgcatt aagcagaacc
    12241 atcggtttga actatgtttt ttaagaggag ggcatatgac acaatattta ttaaaattac
    12301 ttgagcaggg aaaaacgctg actttgcagg acattctgtc acatcttgtt gtagcggtcg
    12361 ctataggtat agtgatatat ctgtcatata tgtacaccca ttcgggaacc atatatagca
    12421 gaaaattcaa tgtatcgcta cttatgctta cattacttac aactatggta atgactgtta
    12481 taggaaacaa tatcgctctt tccttaggta tggtaggcgc attgtctatt gtaagataca
    12541 gaactgcaat aaaagattcc agggataccg catatatctt ctgggctata attgtaggta
    12601 tatgttgtgg agcaggtgat tatctggttg ctgctatagg aagtgcaata gttttcatac
    12661 tgcttcttgt attcggaaag attaagaatg ataacaggat gcttgtgata ataaaaggag
    12721 caagaggttt ggaaaggcag attgaagcca tagtatttaa ctactatttc gctaaagcga
    12781 atcttcgggt aaaaaacacc actgacctca ctgtggaata tatatatgag gtatctaaaa
    12841 agatcatgcg taaagcagca gtaaacgaaa aaagcataac cgataagcta tatgaattac
    12901 agcatataga gtatgtaaat attgtagagc agaatgatga agtgaacaat taaaggagaa
    12961 gcctttgcgt aggaagatta caagcataat agctataata tgccttgttg cgatatctat
    13021 aggatttact tttttaaatt ctgacaacat aagatatcat cagcacctta tggcccaaag
    13081 ccatcaaaac tgtagccatt caacagatga tttctgcacg catatacctt taattaaaat
    13141 tgatacatca agccagcaga tacctgcatt tgaagatgga aagatattta tagaatcaga
    13201 tatctccatt tttgacaaag aagattacaa taatcattta tatgaccaac ctgatataca
    13261 aagcactata ttaataagag ctcgcggtaa ttcttcttta ttttttgata aaaaatcata
    13321 tttcataaag ctaataaacg atgatcatag tgaaaataaa cagaaagtct taggtatgga
    13381 taatcattat gagtgggttc ttcatggacc tattcttgat aagactttaa taagaaacta
    13441 cataagtatg aatctttcag gtcagattat gaaaaccgct cctgatgtgc gcttttgcga
    13501 agtattcata aacgatcaat acatgggtct atatgtcatg atggaatcca tatcacgtgc
    13561 agaaggaaga gtggatatag ccaagtataa tgagaatgat cctttcacca gctatatcat
    13621 aaggctagac agaggttcag accctgaaga agagataacc ctgttttccg gttatacata
    13681 taattctgac tcaattgcta atattgttta tcctccgaag cattatttaa atgaacaact
    13741 taataagtat ataagtgaca atatatctca attcgagaaa gcgctgtatt cgtatgatta
    13801 tgatgatccc aaattaggat accgtaaaca tatcgatgta atgtcttttg tcgattattt
    13861 aattattaat gaatttaccc agaactatga tgcaggcata tattcaacat atcttttcag
    13921 agatattaaa ggaaaattgg aaatagggcc agtatgggat tttaataatg catatgacaa
    13981 ttacatggag actcctataa atacaaccgg attctcattt cagaataaag tatggtacat
    14041 aatgcttatg aaggatgaat attttgttaa tctagttatt caaagataca aacagttaag
    14101 gaaaactgta ttaagcgaag aatatgtaat cggatatata gaagataccg tggaatattt
    14161 gggaccggca attgacagaa attttgaagt ttggggttat acattcttag cagatagagg
    14221 aatgctgttg ccaaaagaca gaaatcacaa gagttatgaa gcagcattga atcaactgaa
    14281 gcagttcata attgatcgag gaaactggat ggatgataat attgattccc tcatgcagtt
    14341 ctgccatgat tcaaggaata aatcatttaa cgactaaggg agaagatgaa aaagtttttg
    14401 ataggaacag tcacaataat tgtattagca ttaagtataa actatatact gttctataca
    14461 agcttctata tagattttaa tccgaaagca acaataacat catcctttaa agctgaaaac
    14521 gaaagtatat atattctaaa agatggtgag tatgagaggt ttgaaattag gggagttaat
    14581 attggcaata cagtaccagg aaaatttggc acagattatg ctatcgacaa gtatgattat
    14641 ttaagatggt tcaagcagat acaggaaatg ggagccaata caataagcgt atatcagata
    14701 atgaatgcag atttctataa tgctctatat cagtataaca aggataatga tgacccgtta
    14761 tacctcatac atggtcttgc catttctgac tattctcaaa atagccatat agatgcgact
    14821 gataaggatt tttatgaaga tattataagg caaatatata tcacagtgga catactgcat
    14881 ggaagcagac aggttatact taaccaggca agcggtttgg gtttttatct tcgtgatgta
    14941 tcacagtggg tgatgggtta tatattaggt tcaggatgga acagttacac aatagcttac
    15001 acaaatgaat tacacaaaga ccattacggt tacagtggta aatatatgta tacaagcgaa
    15061 gatgctacag catttgaatc tgttttagct atgcttggag atacattaat aaagtatgaa
    15121 acggaaagat ataaagaaca aagattagtt tccttcttta actggccttc aacagaccct
    15181 tttgagtatc ctgactacat tacagaattc tttaagaagt gcgctgaagt ggatgttgag
    15241 aacataaaga ctacgaatca attcatgtca ggtcagttcg catcatacca tgcatatcca
    15301 tattatccag gttatctgaa ttattacgaa aataaggaat tctggtttgc tgatgcacaa
    15361 ggggatgtta atacctatac agcttatctg aaaatgctaa tgcttcacca tactatgcct
    15421 gtagttatta cagagtacgg agtaccaaca gcaagaggca tggctcatga ggacactaat
    15481 aaaggatata accagggaag aaattccgaa tttgaacagg gacagataat actagataca
    15541 tataaacaga ttaaaagcgt agattgcgca ggaagtctgc tatttgtatg gcaggatgac
    15601 tggacaagaa ctacctggaa tacccagcac tctgttgatg aaaaaagatc accttactgg
    15661 tcagacgctc aatctagcga tcaggggttc ggcttgttag ccttcgatcc tgggaagaaa
    15721 gaagtattat gtcatgtcga tggcaatata gatgaatggc tcgatatatt accagttgca
    15781 aagactaaag atatgtcatt gttcatgcag tatgatgaaa aatacattta ttttcttgta
    15841 cataaagaaa attataatca cattaatgat gtcctataca tacctataga cacaacggat
    15901 aaatcaggaa gcaattattc tgttgaccat gatgtaagat ttgattctaa tgctgatttt
    15961 ctaatggtta taaatggtaa agaggaatcg agaattctgg tgcaggaaag atatgacact
    16021 ttgagagcaa tgtacaatca atatattgag ggagttaacc cctatactga tcctactaat
    16081 gtaaatagtc caaaatttaa taaaatccag cttatacttc agttagaggc agttgaaaaa
    16141 atcaataatc ctgaacttat ggcatcaacc tatgagacag gtcatctcac atatggaaac
    16201 agtaatcctc atagccaaga ttataactcg ctagcagatt tctatataga aggagattat
    16261 atagagataa ggattccatg gcagttattg aatttttctg atccgtcaaa aatgagaatt
    16321 catgatgatt actaccttca ttacggagtg gagtttacga caataaataa acttcttgtt
    16381 ggaataggat ctgttgatta caaaggacat ataattcata tgaatgagtt cttgctcaaa
    16441 aaatgggatg agtcagttgc atatcatgaa agactaaagc agtcatacta tatgattaaa
    16501 gaagcctgga caaatgagat cttgaaaaaa gaggttcaag gagctgttga tgagtgattt
    16561 aagaattgac atagtgctta taatttacat aatcatatgt gttcttatga tactgtttaa
    16621 tactgccttt ttaatttatc ttagatacaa agattatatt ttcagaaaca aaagaaaaag
    16681 gcttgagaat ataataaaaa gtcaaattaa cagaataagc aaaaacgaag taatagatcc
    16741 gaaaattcaa gagtttcttt ttaaaaaact aatcagtatt cgatatctga aaagttttca
    16801 tgagattatt aaaaatgaaa gtaaaaaaaa tgaaactgat acttttagat accttaagaa
    16861 atgtaaaccc atattcgaat accttatagt ggaattcagt aacaaggaaa atgccaaaaa
    16921 agcatacttg gcatttttaa tatctgagtt caaactgtgt gacaacctcc attacgactt
    16981 tatagtctca tccatgattt cgtataccat gaacaaatcc atttatgtaa gacagaacgc
    17041 gttaaacgca ttgtatatgt ctaataatag tgaagctatt ttatctgcaa ttatgagaat
    17101 gcaggagctt aaaatatcac atagcaacaa acttatatcc gatggtcttc ttttgtttaa
    17161 tggagacaga gcagaacttg catctttgct ttggaaagag tttgacaaat ttgatttgaa
    17221 tgtcaggata gcaatagtaa actttataaa gactgcttca ttagattatg cagaagaatt
    17281 ttatcaaaga ttaactaaca aatatactca caaagaagtc aaactatcat tagtccgtta
    17341 ttttgggaaa atgatttatc agcccgctgg cgactatttg ataactatga tgaagcagga
    17401 tacaccggaa tgggagtaca aagctatagc cgctactgct ttggcaaaat acaagagcca
    17461 acaggttatt gatgttctaa aggaagcgct taaatccgac aactggtata tccggttcaa
    17521 tgcatcagaa agccttgtcg ctcaaaatat tggatatatg gagcttatag atatctacaa
    17581 tggtactgac agatatgcaa gagaaatact ggaatacaaa atggaaactt cccggttaaa
    17641 gaaacaagcg aaagaaggag agtaaatgtt tattgaagca ctaagaatag taatgctgat
    17701 tgtaggagtt ttttactcac tatatcttct tgtatatgcg acattcttag tagtttcagt
    17761 tacagtaggc gcttctgagt tatataagaa ccagcagatg agcaggcttc ataatgagat
    17821 atcacatgaa tactattttc cagtatctat agttgttcct gggtacaatg aggaaataac
    17881 gatatgtgat actgtaaatt cattattaaa tttggaatat aagctttatg aaatagttat
    17941 agtagatgat ggatctaaag ataatacatc caaagttttg attgatactt ttaaaatgaa
    18001 aagagtaaga agaaagataa aaaagagcat aagatgtcag gaagaagaat tcgtgtatga
    18061 atcgtatggg tataaagttc ctatagtatt gataagaaag aaaaacggag gcaaggcgga
    18121 tgctctgaat atgggtataa atgcatcccg ctacccctat tttatctgta tggatgctga
    18181 ctcgatgctg cagcatgatg cactagaaag aatagtaaaa cctgttctga ctgatgagaa
    18241 tcttatagca tgcggagggc ttgtgcgcat tgcaaatggt gttgttttag aaaatggaag
    18301 agtcaagaaa tatgggatgc caaaaaaaat ggttacagca atgcaggttc tggaatatga
    18361 aaggtctttt cttgcctcaa gaatattatt tgatatgttt aatgggaata tgataatttc
    18421 cggagctttt ggattattta aaaaggatat cgtaattgca gtaggcggat atgaccatgg
    18481 gacaatgggg gaggatatgg aacttgttgt aaggcttcat gttttctgca aaaaaaacaa
    18541 cataccatat actataaaat atgaacccga agcggtatgc tggagtcagg ctccagaaag
    18601 tatgtcagac ttaaaaaaac agagaagaag atggcatctc gggctttttc agagtttaag
    18661 caagcatagt caggttcttt taagtactaa gtatggtgca ttaggtactg tttcatatat
    18721 gtactttgta atttttgaac taatgtctcc atatattgag attttcgggt tattttcagt
    18781 tatcataatg tattttcttg gtatgatatc gttaagagta ataataatgt tttctgtact
    18841 ttcaattctt tttggagcag tactgactct gtcagcattc ttttcaagga tacatactca
    18901 gaatgtaaag ctttcctcaa aagatgtatg gaaagccata tgggcttgca tatatgagag
    18961 tgtttggttt agggtaattc ttgcattcac acgtatgaca gcatttatag gatacaagaa
    19021 gaaaaaactg caatggggaa aaataaagag ggttaagctt aatcaatgat gtaaaatata
    19081 tataaaggat atatgggtaa agttatgatt tcatttagag gtattaaaaa agaattattc
    19141 ggatattcga aaaaagatgt tatgaactac attgagatgt taaacgagga tcttaatacc
    19201 aagctttcag ataaagacag acaggtaaag gaagttattg agcagaataa catactacta
    19261 aaaagactgg aaaaactgga aaagactcag gatgaaattg catatgcaat cattcaggct
    19321 aaaaagtctg cagatttaat agtaaatgat gctaacaagc aggctgaaaa aatattgaaa
    19381 gatacaaaag aaaacaagca aaagcaggaa agtgaatata atgaatatat tgaacaaata
    19441 attaaattta aaaaacagat gacaagaata ctgaaaagct ttgtcaaaga agcagaaaat
    19501 attaagaagg aatgtgacga agcttgcaga gtatcctttg ggagggagca tgaatagaag
    19561 agcgataatc atatctgtta tcattatact tcttcttagc gtacatactt tttctggtat
    19621 tgcattagga gaagattttg taatgagtca gatagacaaa gagactgttc agaattatcc
    19681 tgtaatatat gtagcaatag aaaaagcaag cttccctttt gagttctata acaattatga
    19741 aaaacaatac gaaggggtaa tagttcagat attgaaaaaa gtctcctatc ttactggatt
    19801 taaatttgag tacatatata ctaaaaactc aaatgagacc ctgcaatttg catataatat
    19861 gcagactgat ataactacat catttgtaag accatattct ggatttaaag ccggcaggga
    19921 aattgcaagc tttgatggta atatcattgc aatacagtac acaagcagaa tgccgaatca
    19981 agcaattgaa ctcatgaata gagctattga tacgatatca caagaacaga tagtatcaat
    20041 aatttcaagc caagcagtac agtatgaaaa aatgtttaat gataccaaac tgaatatagt
    20101 tatagctact tcagttgcgg ttgtttccat aacagcatta gtatcagcat ttacagctta
    20161 taaaaagtgc aaaaaactca aacagcaatt ccttaacaca gacaagttga cagggtatcc
    20221 aaacttaact ggcttaaagt ctataacaga tataactata agttcactga atataagctc
    20281 atattgtatg gttaacttcg ttgtttctag tttggataat ataagagaga ttttcggata
    20341 tgatgaagca aataaagtac aagtggatgt agctttaatt atagatgagt atatatataa
    20401 taacgaaata ttagcaagag ctgataatga tgatttcatc ttgttattac agtttactga
    20461 aaagcctgag atggctctta gaattaagca gattattgac gccatcaatg ataagtttga
    20521 tataaagaaa tcagagtatc cggtagtttt aagtattggc atatataccc ctaagtatga
    20581 agaagtaaat atggatatgc ttatgcagaa atccttacaa gcaagaagat ttgtaaaaga
    20641 aacagatgaa ataagattcc tgttttttga tgagactgct cacattgtta ccatagaaga
    20701 gaatctttta cagcaggaat ttactacaag catggaaaaa gaagattttc ttatttatat
    20761 tcaacccaag atatctttaa gggataacag catatgtgga ggagagattt tatcaagatg
    20821 gtataaccca agaagagggc ttatacgccc tggtgagtat ataccggtat ttgaaaaaag
    20881 cggatcaatt atcgcttttg atatgtatat gtttgaaaaa gcctgcaagt taattgcgaa
    20941 aagcatgaaa gagggaaaaa gagttgttcc tgttgctata aactttgcac gatctcattt
    21001 tgcagatata aaattctgtg atgatattct ggatattgca caaagatatg aagtgcctgc
    21061 tagtttgtta aatattgaag ttactgaaag tataatatca gaaaataaga aaattatgat
    21121 agaaatagtt aaaagactta aagaatatgg ttttaccatt tctctagatg atttcgggac
    21181 aggttacgcc tctttttctg acctctcaag ttttactata gattatttaa agcttgataa
    21241 gagtctgctc tataatctgg ataatagtag a
//
LOCUS       DAOSXM010000031        21039 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2111
            NODE_1595_length_21039_cov_9.361942, whole genome shotgun sequence.
ACCESSION   DAOSXM010000031 DAOSXM010000000
VERSION     DAOSXM010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864178
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 21039)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21039)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:24:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,261
            CDSs (total)                      :: 2,229
            Genes (coding)                    :: 2,218
            CDSs (with protein)               :: 2,218
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 3 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21039
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1595_length_21039_cov_9.361942"
                     /isolate="AMR_MDS_2111"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            136..1488
                     /locus_tag="PLQ68_05670"
     CDS             136..1488
                     /locus_tag="PLQ68_05670"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurT ligase domain-containing protein"
                     /protein_id="HXK72172.1"
                     /translation="MSVLFCKLSCYLLRLLKKGGTALPGKIALFLCKDILSHVSKNVK
                     VIIVTGTNGKTTTSRIIEQGFIEADLKYFTNKSGANLLSGVVTSFCINSNLKGINKYQ
                     YAVIECDEAALNQVTNFISPQTILVTNIFRDQLDRYGEISHTLNLIAKGINNCKDVTI
                     CINADCSLCTYLINLTLKKTILYGINTYIYDNLAKEPKDALYCLKCRHPYEYDYITYA
                     HLGGFKCTNCDYRRINPEICVTDILNINESGSNVVLKIFDKSYEIRVNLPGAHNIYNA
                     TAAACALHSIQVEDKFILSSISNFQSGFGRMEKIFINSIPVNIILVKNPAGFNQILNY
                     FSIIDKPFVHVIMLNDLSADGTDVSWIYDVSFNKLLNISKMLKHIYIGGKRAYDMALR
                     LKYEGFDMSMVSIEQDNEKLINVALNHNVPVYITPTYTAMFELRNMLVKKYGLKEFYE
                     "
     gene            1485..2234
                     /locus_tag="PLQ68_05675"
     CDS             1485..2234
                     /locus_tag="PLQ68_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004626924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HXK72173.1"
                     /translation="MSDTIRICHLYPDILNLYGDYGNVLCIQKRLLWRNINCIIENVT
                     VKDKLYPENYDMFFIGGGQDFEQEILLEDLLVNKKELIKKAVDDNKTFLAICGGYQLL
                     GKYYKTWDGQNLEFVGALDIHTIGQKKRMIGDYKFTCQDDDGYFEVIAFENHSGKTYL
                     SKSVNSLGQITKGNGNNGEDNTEGARYKNVFCSYGHGPLLPKNPRLADLIISTFMKQR
                     IKDFKLSKLDDSIENLARNNMIERVRFHSQR"
     gene            complement(2212..3576)
                     /gene="trmFO"
                     /locus_tag="PLQ68_05680"
     CDS             complement(2212..3576)
                     /gene="trmFO"
                     /locus_tag="PLQ68_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HXK72174.1"
                     /translation="MERVAKKIKINDSNICINVVGAGLAGCEAANYIANKGYIVNLFE
                     MKPQEFTRAHKSSDYCELICSNSFKNKSITSATGLLKQELIRIGSLILKCAYDNELPA
                     GSALAVDRDNFSKQVTYSIKSNPNINIVTKRVNSIDELIVNDSTYTIICSGPLTTDNL
                     ADSIMSTIGDNNLYFYDAIAPTLQFDEIDFNYAYMASRYSSEDKSYINCPLTKEDYDK
                     FYNFLVSAETVKLKKFEEQKVFEGCMPVEVLAKRGYDSLRYGPMKPVGLTNPHTNSKD
                     YAVIQLRQDNLKASIYNMVGFQTNLTFPMQQQLLKYIPALKNCKILRFGVMHRNTYLD
                     SPNILNKFFQMKSNKNVFFGGQITGVEGYLPSTASGLVAAINCIKEIRGKNKVDFSSS
                     TVIGSLQNYISTPPIKKFLPMNANYGIMTPLPENIRNKTKKYEEYSKRSLEVLEKIIS
                     DYEI"
     gene            complement(3545..5674)
                     /gene="topA"
                     /locus_tag="PLQ68_05685"
     CDS             complement(3545..5674)
                     /gene="topA"
                     /locus_tag="PLQ68_05685"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HXK72175.1"
                     /translation="MTKNLVIVESPAKANTIKKFLGNGYTIMASYGHVRDLPEKIFGI
                     DTENNYLPKYQILPDKKEVVEKLKKAVKDSDNILLATDPDREGEAISYHLAHILDIDP
                     ESKCRIVFNEITKQAVKNAITSVRPINLDMFNAQQARRVLDRIVGYKLSPLLWKKIVK
                     GLSAGRVQSVAVELICDKEAEINAFIPEEYWNLILKLSKIDDDQIFIAKYYGNDKSKV
                     KVTNEKQATDIYEYVKNNVVSVKSVKKTKRKRYPYAPFITSTLQQDASNKLGFTASRT
                     MRVAQSLYEGVEVKDVGSVGLITYMRTDSTRISPDFQLATRDFIKKNFGSDYVPNSIP
                     TYKNSNNSQDAHEAIRPTEYNLTPSKVSSSLSKDQAALYTLIYNRYIASQMPPAEMDN
                     TCVDIVCGPYIFKSCGNIITFKGFLTVYDEQKTETNDKDQDEYENTYLPKLTAKEQLE
                     KHDLQKIQKFTNPPARYTEASLVKALEEKGIGRPSTYAQIIEVIIKRNYVKREKRSLI
                     PTYLGINVNDFLVENFTSLLDYGFTADMENKLDEIESGKVDWVSLIDNFYIDFINQIN
                     DVDKNTQKISISLNRQCKLCGKDMVLKYSKFGFFAGCSGYPECKYTEGVDDNTSGVTC
                     PKCKGNVIHKKTKKGKPFLSCENYPDCDFSSWGTIVKNRNCPKCDNFLVKLYEEKKPV
                     IRCSKPECDYLEKDIKSYGKGSKKNKD"
     gene            complement(5622..6464)
                     /gene="dprA"
                     /locus_tag="PLQ68_05690"
     CDS             complement(5622..6464)
                     /gene="dprA"
                     /locus_tag="PLQ68_05690"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HXK72176.1"
                     /translation="MSKNELTQLSVKTGILTDKLLKTKEFKIKPYNKYKNVELIDIYS
                     NKYPYLLKQTFGAPIVLYCIGNIPQKQMISIVGSRNPSEYGKRNAYILSDNLSLQGYC
                     ITSGMAKGIDGIGHMAAIKNEVSLAVLGNGIDICYPECNRFIYNNLLNNGCIISEYPP
                     GTKPQKSFFPQRNRIISGLSFCTIIIEASERSGSLITANLALSQNREVYCLPNLIDSG
                     YNGTNCLIKDGAGIITDIGQFCQELKAIEISWKNLYDCKSSLGGNYDKEPGNSGITRE
                     SQHN"
     gene            complement(6613..6954)
                     /locus_tag="PLQ68_05695"
     CDS             complement(6613..6954)
                     /locus_tag="PLQ68_05695"
                     /inference="COORDINATES: protein motif:HMM:NF018753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HXK72177.1"
                     /translation="MVVNDLITILKGRCIASESKINREIQYVFCCDTLKVVEDKASCD
                     YAWVNSNVDKTAIDLAVRKALSCIIVPYDITMDEDILRYAEQNEIPIVSTGYSSYKVC
                     SKIYEAFKERT"
     gene            complement(7030..8769)
                     /locus_tag="PLQ68_05700"
     CDS             complement(7030..8769)
                     /locus_tag="PLQ68_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent [FeFe] hydrogenase, group A6"
                     /protein_id="HXK72178.1"
                     /translation="MNLLNVTIDGIKVQVPEGTSVLDAAKLANVDIPTLCYLKDINEI
                     GACRMCLVDVGARALQTACVYPCSEGLNVITNSPKIRDIRKATLELILSNHERTCLTC
                     IRNNNCELQALAEKLNVREIPYDGVSKGLPIDNISKSIVRDNNKCILCRRCVAVCNKI
                     QTVGAINVMERGYDTIISTADHRSIADVNCVNCGQCINVCPVGALYEKDNTQLVWDAI
                     KDPDKHVVIQTAPSVRAGIGEEFGYPIGTPVTGKMVAAIKKLGFDRVFDTDTAADLTI
                     MEEGTEFLNRLNNGGKLPLITSCSPGWVKFCEHEFPDFLDNLSSCKSPQQMFGALIKS
                     YYAKKNNIDPSKIFVVSVMPCTAKKYEGDRQEMEKDVDISITSRELARMIREAGVDFR
                     SLEDAKFDDPLGEATGAGVIFGATGGVMEAALRTVYYILEGKRINELDVKPIRGTEGI
                     KEATIKIAGIDVSVCAVSGLGNARKVLNMVKNGEKNYHFIEIMACPGGCVNGGGQPIV
                     PAKIRNKINVAAERAKAIYSEDQRATYRNSDENEFVKKFYEEFAEAPGSHIAHELLHT
                     HYIKRDRFIDKSK"
     gene            complement(8782..10545)
                     /gene="nuoF"
                     /locus_tag="PLQ68_05705"
     CDS             complement(8782..10545)
                     /gene="nuoF"
                     /locus_tag="PLQ68_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004614050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HXK72179.1"
                     /translation="MATCGIAAGANPVMSEFKKIAQEKKLDNVNIQLTGCIGICRLEP
                     LVEVYDSKGNRFTYIHMDSKKAAEVMQSHIIEGKPCTDYLICDEPNGCIKNVLETRSF
                     KKQIRIALRNCGNINPEHIDEYIQNDGYSALGKVLTEMKPQEVIQLLLDSGLRGRGGA
                     GFPTGLKWKFAAAAQNDQKYVCCNADEGDPGAFMDRSVLEGDPHSVLEAMAIAGYAIG
                     SNQGYIYVRAEYPIAVKRLNIAIKQAKEYGLLGNNIFGTGFDFDIEIRLGAGAFVCGE
                     ETALMTSIEGKRGEPRPRPPFPANKGLFGKPTILNNVETLANVPVIITKGPQWFNKIG
                     TEKSKGTKVFAVGGKINNTGLIEIPMGMTLREVIYDIGGGIPNGKAFKAAQTGGPSGG
                     CIPAEHLDIQIDYDNLVAIGSMMGSGGLIVMDEDNCMVDIAQFFLGFTVDESCGKCPP
                     CRIGTRRMLEMLEKITDGNGEDGDIEKLERLAYSIKEAALCGLGQTAPNPVLSTLRYF
                     RDEYKAHIYDKTCPSGVCKHLLNYYIDSEKCVGCSVCARNCPVNAISGVIKSPYVIDN
                     DTCIKCGVCYEKCKFDAIYKK"
     gene            complement(10641..11132)
                     /gene="nuoE"
                     /locus_tag="PLQ68_05710"
     CDS             complement(10641..11132)
                     /gene="nuoE"
                     /locus_tag="PLQ68_05710"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014314201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HXK72180.1"
                     /translation="MSEVNLMCQNCNLDEKEMKLQEVLNKHKDSKGALIPVLHEAQEI
                     YGYLPLSVQEVIANALGLPLSEVYGVVTFYTQFSLYPKGQYKIQVCLGTACYVKGSNN
                     ILEEFKKQLGIEVGQSTEDGKFSLDACRCVGACGLAPVVMINEDVYGTLVPGDVANIL
                     KKY"
     gene            complement(11253..12380)
                     /gene="nagA"
                     /locus_tag="PLQ68_05715"
     CDS             complement(11253..12380)
                     /gene="nagA"
                     /locus_tag="PLQ68_05715"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HXK72181.1"
                     /translation="MIIKNCRIFNGLKFLEGLNDILIKNNKIIGIGKNIKNIEDEEIV
                     NIDGNFAVPGYIDIHTHGINGIDFSTSYNYNTDDLFNFYVSKGTTSVYLTTVTMSLED
                     IIKLLKRFSIVNHAVFRGVHLEGPYLNISKLGAHKKEFVIEPSVSSYKQVVGKYHDIV
                     KRVTIACELDKNYLLAKYLIKNGVVVSFGHTICNAEDSYKAFENGYRLSTHHFNAMPL
                     MHHRDVSITGTSLLNDNVKCEYIPDFYHVSKEMLNILFKCKPEDNLIMVTDSISATCL
                     EDGQYSLGGLSVSVHNGLVTDKSGTIAGSSITMAEGVFRMINNGFNKSSVLKSATSNP
                     ANVMSLKNKGFLKEGYDADINILEKNFKYNYTIFQGKLFNY"
     gene            complement(12391..14403)
                     /gene="recG"
                     /locus_tag="PLQ68_05720"
     CDS             complement(12391..14403)
                     /gene="recG"
                     /locus_tag="PLQ68_05720"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HXK72182.1"
                     /translation="MNELTSVKGIGPKANEKLNSLGISSIKDLLEYYPRSYIDRSTIS
                     NVSNANESEYIALKGIITNIISKKLPYGRKLHITTAFFRDKTGQIELTWFNQPYLKNI
                     LKEDKTILIYGKIEKKGFFLKMNNPDFSFNESDFFTIQPIYPKNRYTTQYDIRKSVKS
                     AFQYIDNLIVDEFSIDLRKKYSLCEKKYAIKNIHFPNSSEDFQLARRRLVFEEFFIMQ
                     LALFSLKQNIKNEKTKIPFLKYKAADEFVSKLPFSLTNSQKQAITELKNDLLSGYPMN
                     RLLQGDVGSGKTVVAFYCVMSAFSNSYQSAYMAPTEILAMQHYQTAQSLLQGLGIRTY
                     LLTGSMKKAEKEETLNKIVNGEADFVIGTHALIQDDVVFKNLGLCITDEQHRFGVAQR
                     SILSSKGDKPHLLVMSATPIPRTLALIIYGDLDVTSITELPKNRKKVLTYSVDETYRK
                     RINDFILKIVSLGEQVFVVCPAIYENNDIDITNVTEKHKKLLEDLPGISIGMLHGEMN
                     NQQKNEVINKYLNNDIKVLVTTSIIEVGIDIPNATLIIVEDAQQFGLSQLHQLRGRVG
                     RSDKQSYCVLFNQSESDISKKRMNAMVNSNDGFYISQQDLLLRGPGDFFGLKQHGLPD
                     FKIANIYEDMDLLKLSQKAVKDELGKIYLYERLIKDSINNLSKTGL"
     gene            complement(14400..15143)
                     /locus_tag="PLQ68_05725"
     CDS             complement(14400..15143)
                     /locus_tag="PLQ68_05725"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019213490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034979 - NAD-dependent protein
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HXK72183.1"
                     /translation="MKIDNLVEKIKNSKSIVFFTGAGVSTESGIPDFRSSKGLYKERY
                     QGYSPEHILSHNFFYEHTDIFYKFYFDNIVHIDAKPNKFHKNITKLEKMGYDVAVITQ
                     NIDNLHNEAENTNVYELHGSINRNYCTSCKKFFNLDYMIKNTNNIVKCDYCNSLVKPD
                     VVLYEEQLDESVIENSISKILKTDVMIVAGTSLSVYPAASFIDFYKKDCLCIINKTTT
                     LHDDRADYIFYDSCSVILEKLVNKLENQP"
     gene            complement(15140..16009)
                     /locus_tag="PLQ68_05730"
     CDS             complement(15140..16009)
                     /locus_tag="PLQ68_05730"
                     /inference="COORDINATES: protein motif:HMM:NF013431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HXK72184.1"
                     /translation="MVKLKNREISVEINPIGAEIVSIKKNGYEYLWQKNPNIWNGQAP
                     NLFPIIGRLKDEEYEHEGRMYNIKIHGFVKHSMFDITENTENYVSFMLSANTETYSSY
                     PFDFDLIITYELMNESIKKQYIIINKGYKPMYFEVGGHEGFNLTFSENKVMDDYYIEF
                     FDNDIYSYTTDKDIMLNKDLKKIILNNKKLYLNPQIFTNDALILDCSKLNTRSVTLKC
                     DGSDRSITVNFDDFDYLGIWSRPTYSNYVCIEPWSSLPDCNYIDKKIENKIGVKSLSP
                     NKTDILSYIIEVR"
     gene            16098..16325
                     /locus_tag="PLQ68_05735"
     CDS             16098..16325
                     /locus_tag="PLQ68_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013290567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HXK72185.1"
                     /translation="MKHTIDIKFLSISDVKEFADIVSAFNYDIDLISGRYVVDAKSIM
                     GIFSLDLSKPISLVAHTDNVLPLAEAIKKFC"
     gene            complement(16429..17238)
                     /locus_tag="PLQ68_05740"
     CDS             complement(16429..17238)
                     /locus_tag="PLQ68_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HXK72186.1"
                     /translation="MTECNHNCESCSQDNCESRIDKEKANDFSQIKNVVAVVSGKGGV
                     GKSMVTSLLAVELTRLGYKVGILDADITGPSIPKSFNINDKVMGNESGIFPAKSSLGT
                     KIISINLLLENNTDPVLWRGPIISGVLKQFFTDVIWGELDYLLIDMPPGTADTALTIF
                     QSLPVDYALIVTSPQDLVSMIVEKAIKMADMMNISVLGAVQNFSYVICDKCGNKIELF
                     GNESGNNLSIPILDRIPFDKKLSSAIDRGMIELTDNSYLSNTINELINKQI"
     gene            complement(17250..18251)
                     /locus_tag="PLQ68_05745"
     CDS             complement(17250..18251)
                     /locus_tag="PLQ68_05745"
                     /inference="COORDINATES: protein motif:HMM:NF020650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXK72187.1"
                     /translation="MKKRTSKTIICIVLAIIAMISLAGCNKIYETNPPEDPITAKIVA
                     MKGPTGIGMVRVMNEIVSLSDYVNTDFEIMTSTDTVVAGLLDKSITIAAVPTNLASVL
                     YNRTEGEIQILAVHTLGVLHILDKTGTINSIEDLKGKTITASGQGSLPQYVLEFILNE
                     NGIVPQTDVEIIYKAEHSEVATLLLSGEVDIVMLPEPFVSTVMNKDESIIHAIDLNEE
                     WTEKAPDITLSMGCLVAQKTFIEEYKEEVNTFLDLYKESIEWVLEKPYEASELIVTSG
                     ILDNETVAEKAIPNCNIVYIDAADAKDSLDAFFEFLFENNPSSVGGKLPDQSIYYIK"
     gene            complement(18308..20302)
                     /gene="ligA"
                     /locus_tag="PLQ68_05750"
     CDS             complement(18308..20302)
                     /gene="ligA"
                     /locus_tag="PLQ68_05750"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013403035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HXK72188.1"
                     /translation="MFMKKRMDELVELLNYHTKKYHEEDSPEISDFDYDMMLNELIKL
                     EEKYPEYVSINSPTKRIGGQPLKSFDKVEHIVPMESLSNAFSYDDLYAFNQRILKEGI
                     EPEYVLEKKIDGLSVSLEYENNIFVRGSTRGDGVFGENITNNLKTIPSIPLRLSHNEI
                     IPYIEVRGEVYMDKDAFKKINEKAVETGDKEFANPRNAAAGSLRQLDPSITAKRKLKI
                     FIFNIQAIQGTIFKTHSEALTFLRKIGFETNEIIVISKNLEEIIKGIEKIQIERHTYK
                     HDIDGAVIKLNNLEDRKVLGSTAKAPRWAIAYKYPPEQKETLVKDIFVQVGRTGVLTP
                     NAIFDPVVVSGSRISKATLHNLDFIRENDIRINDRVIIQKAGDVIPEVVKVLKEKRTG
                     NEIIFNMPEKCPLCNSKVVKMEDKSAYKCIGINCIGQISRKLEHFCSKDAMDIEGLST
                     GIIDKFMNEGFIRTIDDIYNLHSNRKELIKISGFGTKSIDKLLNAIEKSKNNNIDRLI
                     FGLGIEHIGKKASSLIADKFKDINSVMTADELSFTEIDTIGFIMAKSIVQYFRNPEAV
                     ILINNLKEKGINMNSLNYGKSGNSRLKGITFVITGSFEDYSREDLKSLITSFGANVTD
                     SVSKKTNYVIVGENPGSKIDKADKLGIKTLNIEELKKTFL"
     gene            20397..20648
                     /gene="rpsT"
                     /locus_tag="PLQ68_05755"
     CDS             20397..20648
                     /gene="rpsT"
                     /locus_tag="PLQ68_05755"
                     /inference="COORDINATES: protein motif:HMM:NF013787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HXK72189.1"
                     /translation="MANSKSALKRVRVSSKKKLQNSSKRSSLRKTMKRANTVIENEEK
                     DNEAILKAVKAVDQAVSNGIVHRNKGARDRSKLIKALYK"
BASE COUNT         6698 a         3548 c         2816 g         7977 t
ORIGIN      
        1 agactaaaat ccgtttccgt attttaatac tggaatttaa gttttcactt tacgaaactc
       61 aactaagtat atttaatatt attgataaat tgtaatgtga ctgatataat tcatgtaatg
      121 aaattgaatg tttttttgtc agtattattt tgtaaattat catgctatct gcttaggctt
      181 ttaaaaaaag gtggaacggc tcttccagga aagatagcac tttttctatg taaagatatt
      241 ctgagtcatg tttcaaaaaa tgtaaaagta ataatagtga caggtacaaa tggcaaaact
      301 acaacttcaa gaattattga acagggattc atagaagctg atcttaaata ttttactaat
      361 aaatcgggtg ctaacctttt aagtggtgtt gttacttctt tttgtattaa ttcgaattta
      421 aaaggaataa ataaatatca gtatgcagtt attgaatgtg atgaagcagc attgaatcag
      481 gttactaact ttatttcgcc tcaaacaata ttagttacca acattttcag agaccagctt
      541 gacaggtatg gtgaaatttc gcatactctt aacttaattg caaaaggtat taataattgc
      601 aaagatgtaa caatttgcat aaatgctgat tgctcattat gtacctattt aataaatcta
      661 acattaaaga aaacaattct atatggaata aatacatata tttatgataa tttggcaaaa
      721 gagccgaaag atgcactgta ttgcttaaaa tgcagacatc catatgaata tgactatatc
      781 acctatgccc atttaggagg ctttaaatgc actaactgtg attacagaag aataaatcct
      841 gaaatatgtg tcacagatat attgaatata aacgaatcag gaagcaatgt agttttaaaa
      901 atatttgata aatcttatga gataagagtc aatcttccag gagcacataa tatatataat
      961 gcgacggcag ctgcttgcgc gctacattca attcaagtag aagataaatt cattctttca
     1021 tcaatatcaa attttcaaag cggattcggt agaatggaaa agatatttat taattccatt
     1081 ccggtgaata ttatactagt taaaaatcct gctggtttta atcagatact taactatttt
     1141 tcaattatag acaagccatt tgttcatgta attatgctta atgatttatc tgcagatgga
     1201 acagatgtat cttggatata tgatgtttca tttaataagc tgctaaatat atcgaaaatg
     1261 cttaaacata tttacattgg tggtaaaaga gcttatgaca tggctttaag gctaaaatat
     1321 gaaggttttg atatgtctat ggtatcaatt gaacaagata acgaaaagtt gataaatgtt
     1381 gctctgaacc ataatgtgcc agtctatata actccaacat acacggcaat gtttgaactt
     1441 aggaatatgt tagtaaaaaa atacggacta aaggaatttt acgaatgagt gatactatta
     1501 gaatatgtca tctttatcct gatatattga atctttatgg tgattatgga aatgtactat
     1561 gcatccaaaa aagattgctt tggagaaata tcaactgcat aattgaaaat gtaactgtaa
     1621 aagataaact ttatccagaa aattatgaca tgttctttat aggtggcggt caggactttg
     1681 agcaggaaat attacttgag gatcttcttg ttaacaagaa agaacttata aaaaaagcag
     1741 ttgatgataa caaaacattt ttagcaatct gcggaggtta tcagcttttg gggaaatatt
     1801 ataagacatg ggatggacaa aatcttgaat tcgtaggagc actagacatt catactattg
     1861 gtcaaaagaa acgaatgatt ggagattaca agtttacatg tcaagatgat gacgggtatt
     1921 ttgaagttat agcatttgag aatcattccg gtaaaacata tttatctaaa tctgtaaaca
     1981 gtctgggaca aataactaaa ggaaatggaa ataacggaga agataatact gaaggtgcaa
     2041 gatataaaaa tgttttctgt tcatatggac atggtcccct cttgccaaag aatccaaggt
     2101 tagcagactt gataataagt acttttatga aacaaagaat taaagacttc aaattatcaa
     2161 aacttgatga ttcaattgaa aatcttgcta ggaataatat gattgaaaga gtcagatttc
     2221 atagtcagag ataatttttt ctaatacttc caaagacctt tttgagtact cttcatattt
     2281 ttttgtttta tttctaatat tctcaggtaa tggagtcatt attccatagt ttgcattcat
     2341 tggaagaaat tttttaatgg gtggagtaga tatgtagttt tgtaaagaac caataacagt
     2401 tgatgaggaa aaatctactt tatttttacc tctaatttct tttatgcagt ttattgcagc
     2461 tacaagacca gaagctgttg aaggaagata tccttcaact cctgtaatct gacctccaaa
     2521 aaaaacattt ttatttgatt tcatctgaaa aaatttatta agtatatttg gagaatcaag
     2581 atatgtattt ctatgcatca caccaaatct taaaatttta caattcttta gtgctggaat
     2641 atatttgagt aattgctgtt gcatgggaaa tgtaagattc gtctgaaatc caaccatatt
     2701 atatatacta gcttttaagt tgtcctgtct taactgtatt acagcataat cttttgaatt
     2761 agtgtgtgga tttgttaatc ctacaggttt cattggtcca taccgtaaag agtcataacc
     2821 tctttttgct aaaacctcaa caggcataca tccttcaaaa actttctgtt cttcaaactt
     2881 cttaagtttt acagtttctg ctgatactag aaaattgtaa aacttatcgt aatcctcttt
     2941 tgttaaaggg caatttatat atgacttatc ttcactagaa tatcttgatg ccatataagc
     3001 ataattaaaa tcaatttcgt caaattgtaa agttggagct attgcatcgt aaaaatataa
     3061 gttattatca cctattgtac tcataatact atctgctaaa ttatcagttg taagaggacc
     3121 agaacaaata attgtatagg ttgaatcatt gacaattagc tcatctatac tattaactct
     3181 ttttgtaacg atattaatat ttggattact ttttattgaa tatgtaactt gtttcgaaaa
     3241 attatctctg tcaacagcta atgccgaacc tgcaggcagt tcattatcat atgcgcattt
     3301 caatattagt gaacctatac gaattaattc ttgcttaagt aaacctgttg ctgatgtaat
     3361 ggatttattc ttaaatgagt tactgcatat aagttcacaa tagtctgacg atttatgagc
     3421 tctagtaaat tcttgaggtt tcatttcaaa aagattaaca atatatcctt tattagcgat
     3481 atagtttgct gcttcgcatc cggctaaacc ggcacctaca acattaatac aaatattact
     3541 atcattaatc tttatttttt ttgctaccct ttccataact ttttatatct ttttcgagat
     3601 aatcgcactc tggctttgaa cagcgtataa caggcttttt ttcctcatat agttttacta
     3661 aaaaattatc acattttggg caatttctat ttttaacaat tgttccccat gatgaaaaat
     3721 cacaatctgg ataattttca catgaaagaa aaggttttcc ttttttagtt tttttatgaa
     3781 taacattacc tttgcattta gggcatgtta caccagatgt gttgtcatct actccttcag
     3841 tatacttgca ctcaggatat cctgaacaac cagcaaaaaa tccaaattta ctatatttta
     3901 ataccatatc tttcccacat aatttacact gacgattcag tgatatggat attttttgag
     3961 tattcttgtc tacatcatta atttggttta taaaatcaat ataaaaatta tctataagag
     4021 atacccaatc cactttacca gattctattt catctagttt gttttccata tcagcagtaa
     4081 aaccataatc aagtaaagat gtaaaatttt ccaccaaaaa atcatttaca ttaattccta
     4141 aatatgtagg tatcaatgat ctcttctctc tttttacata atttctcttt ataataactt
     4201 caataatctg agcatatgtg cttggtctgc ctataccctt ttcttcaagt gctttaacta
     4261 atgatgcttc tgtgtatctt gcaggaggat ttgtaaactt ctgaatcttt tgcaaatcat
     4321 gcttttcaag ctgttctttt gctgttaact taggaagata agtattttca tattcatcct
     4381 gatctttatc atttgtttca gttttctgtt catcataaac agttaaaaat cctttgaaag
     4441 ttattatatt tccacatgac ttaaaaatat acggtccaca tacaatatca acacatgtat
     4501 tatccatctc agctggaggc atctggcttg ctatatatct gttgtatatt aatgtatata
     4561 gtgctgcttg atctttgctt aggcttgaac tcacttttga tggtgtgaga ttgtattcag
     4621 taggtctaat agcttcatgt gcatcttggg agttattaga atttttgtat gttggtatgc
     4681 tatttggtac ataatcagaa ccaaaattct tttttatgaa atctcttgtt gctagctgaa
     4741 aatcaggtga tattctagtt gaatctgttc tcatgtaggt tataagtcct actgaaccta
     4801 catcctttac ctcaacacct tcatataatg attgagcgac tctcatagtt cttgatgcag
     4861 taaaaccaag tttatttgaa gcatcctgtt gcaaagttga tgtaataaat ggtgcatatg
     4921 gatatctttt tcttttagtt ttctttacag attttactga tacaacatta ttcttaacat
     4981 attcatatat atcagtagct tgtttttcat tagtaacttt tacttttgat ttatcattac
     5041 cataatattt ggcaataaat atttgatcat catcaatttt gcttagttta agtataagat
     5101 tccagtattc ctctggaata aaagcattta tttccgcttc tttatcacat attaattcaa
     5161 ctgcaactga ttgtactctt cctgcactta agcctttaac aatttttttc cacagtaaag
     5221 ggcttaactt atagccaact attctgtcta atactcttct tgcttgttga gcattgaaca
     5281 tatctagatt aataggtctt actgatgtaa tagcattctt aacagcttgc tttgttattt
     5341 cattaaatac tatgcgacat ttagattcag gatctatatc taatatatga gccaagtgat
     5401 atgaaatagc ttcaccttct ctgtcagggt cagttgctaa taagatgtta tcactgtctt
     5461 taactgcttt tttaagtttt tcaactactt cttttttatc gggtaatatc tgatatttag
     5521 gtagatagtt gttttccgta tcaataccga aaatcttttc cggtaaatcg cgtacatgac
     5581 cataactggc cattattgta tatccattgc ctaaaaattt cttaattgtg ttggctttcg
     5641 cgggtgattc cactattacc aggttctttg tcataattac ctcccaaact acttttacaa
     5701 tcataaaggt ttttccaact aatttcaata gcttttaatt cttgacagaa ttggcctatg
     5761 tcagtaataa tacctgcacc atcttttatt aggcagtttg ttccattgta tcctgagtca
     5821 attaagtttg gcaaacaata tacttctctg ttttgcgata aagctagatt tgcagtaatc
     5881 aatgatccgc ttctttcaga agcttcaatt ataattgtac aaaaagaaag acctgaaatt
     5941 attctgttac gttgagggaa aaaacttttt tgaggttttg ttccaggtgg atattcactt
     6001 ataatacatc cattatttaa taaattattg tagataaacc tgttacattc agggtaacat
     6061 atatcaattc catttcctaa aactgctaat gaaacttcat tttttattgc tgccatatga
     6121 cctatgccat ctattccctt tgccatacca ctagttatac agtaaccttg taaagacaag
     6181 ttatctgata agatatatgc atttctttta ccatattcac ttggatttct tgaacctact
     6241 attgatatca tctgcttttg tggaatatta ccaatgcaat ataaaactat tggagctcca
     6301 aatgtttgtt ttaataaata tggatactta tttgaataaa tatctattag ttctacattt
     6361 ttatacttat tgtaaggttt tattttaaat tctttagttt ttaataattt atcagttaat
     6421 atccctgttt ttactgatag ttgtgtaagt tcattttttg acattgaaaa aatatcataa
     6481 atgctattgg tttcaatata caatttgtat ttttcattat aatttaaaaa atcaatacaa
     6541 gaaaaactat atcttaaatc attcattact aaatgatatt atgttttttt gtattaatga
     6601 atagacaatc tgttatgttc tttccttaaa tgcttcatat atttttgagc agaccttgta
     6661 actgctataa ccagttgaaa ctattggaat ttcattctgt tcagcatatc ttagtatatc
     6721 ttcatccatt gtaatatcat atggtacgat aatacatgac aaagcttttc ttacagccaa
     6781 gtcaatagca gttttatcaa cgtttgaatt aacccatgca taatcacatg aagctttatc
     6841 ttctactact tttaaagtgt cgcaacaaaa aacatattgt atttctctgt tgatttttga
     6901 ttcagatgca atacagcgac cttttaatat agtaattaaa tcatttacca ccatatttat
     6961 caccgccttt aattacatat taacataaaa aaagcggtat ttctaccgct ttaattttat
     7021 gttttaattt tacttacttt tatcaataaa tctgtctcgt tttatgtaat gtgtatgcaa
     7081 cagctcatgt gctatatgac ttccaggagc ttcagcaaat tcttcataga atttcttaac
     7141 aaattcattt tcgtcagaat ttctataagt agctctttga tcttcagaat aaattgcttt
     7201 agctctttct gctgcaacat taatcttatt tcttattttt gctggaacta taggttgtcc
     7261 tccaccgtta acacatccac caggacatgc catgatttcg ataaaatgat aattcttctc
     7321 accattttta accatgttaa gaacttttct tgcattacct aatccgctga ctgcacaaac
     7381 actaacatca atgccagcaa tcttaattgt ggcttcttta attccttctg tacctctaat
     7441 cggctttaca tcaagttcgt taattctttt accttcgagt atgtaataaa ctgttctaag
     7501 agcagcttcc ataacaccac ctgttgcacc aaatataact ccagcaccag ttgcttcccc
     7561 aagaggatca tcaaatttag catcttcaag agatctgaaa tcaacacctg cttcacgtat
     7621 cattctagca agttctctag aagtaataga aatgtcaaca tccttttcca tttcttgtct
     7681 gtcaccttca tactttttag ctgtacaagg cattactgaa acaacaaaaa tcttagatgg
     7741 gtcaatatta ttcttcttag cataatatga ttttattaat gctccaaaca tctgttgagg
     7801 agatttgcat gaagataaat tatctagaaa atcagggaat tcatgttcac agaatttaac
     7861 ccatccagga ctacatgatg taattagtgg aagtttgcct ccattgttaa gtctgttaag
     7921 aaattctgtc ccctcttcca ttatagtaag atctgctgca gtgtcagtat caaatactct
     7981 gtcaaaccca agtttcttta tagctgctac cattttacca gttacaggtg tacctatcgg
     8041 atatccgaat tcttcaccga ttcctgctct tactgaaggt gctgtttgaa taaccacatg
     8101 tttatcagga tctttaattg catcccaaac aagctgagta ttatcttttt catagagtgc
     8161 tcctacagga cagacattaa tacactggcc acaatttaca cagttaacat ctgctattga
     8221 acgatggtca gcagttgata ttattgtatc atagcctctt tccataacat ttatagctcc
     8281 aactgtttga attttattac aaactgccac gcatcttctg cataatatac atttattatt
     8341 atctcttact attgattttg atatattatc aattggtaat cctttagaaa caccatcata
     8401 aggaatttct ctaacattta acttttctgc aagcgcttga agttcacagt tattattcct
     8461 tatacatgtt aagcatgttc tttcatgatt tgaaagaatt agctcaagtg ttgcttttct
     8521 tatatctcta atttttggtg aattagttat tacattaagg ccttctgaac atggatatac
     8581 acaagctgtt tgtaaagctc ttgctccaac atctactaag cacattctac aagcgcctat
     8641 ttcattaata tcttttaaat aacaaagtgt cggtatgtca acatttgcta attttgcggc
     8701 atctagaacc gatgttcctt caggaacctg aacttttatg ccgtctattg taacattaag
     8761 caaattcata aattatcctc cttatttctt ataaatagcg tcgaacttgc atttttcata
     8821 gcaaacgccg catttaatac atgtatcatt atctataaca tatggtgact tgattacgcc
     8881 cgatattgca ttaacaggac aatttcttgc acacacacta catccgacac atttttctga
     8941 gtctatataa tagttcagaa gatgtttaca tactccagat ggacatgttt tatcatatat
     9001 atgagcctta tattcatctc tgaagtatct taatgttgaa agaacaggat ttggagcagt
     9061 ttgtcctaaa ccgcaaagag cagcttcttt aattgaataa gctaatcttt caagtttttc
     9121 aatatcacca tcttccccat ttccatcagt aatcttttcc aacatttcaa gcattcttct
     9181 tgtacctatt ctacatggag gacattttcc acatgattca tccacagtaa aacctaagaa
     9241 gaactgagct atatcaacca tacagttatc ttcatccata actataagtc cgcctgaacc
     9301 catcattgaa ccaattgcta ctaaattatc ataatcaatt tgaatatcaa gatgttctgc
     9361 aggaatacaa ccgcctgaag gacctcctgt ttgggctgct ttaaaagcct taccattagg
     9421 aattcctcct ccgatatcat aaattacttc tctaagagtc atacccattg gtatttcaat
     9481 aagtcctgta ttatttattt ttccacctac agcgaaaact tttgttcctt tacttttttc
     9541 tgttcctatc ttattaaacc attgtggacc tttagtaatg atcacaggta catttgctaa
     9601 cgtttcaaca ttattcaata ttgttggttt accaaataaa cctttatttg ctgggaatgg
     9661 aggtctaggt cttggttctc ctctttttcc ctcaatcgat gtcattagtg cagtttcttc
     9721 tccacataca aatgcacctg ctccaagtct gatttctata tcaaaatcaa atcctgtacc
     9781 aaatatatta ttgcccaata atccatattc ctttgcttgt tttatagcta tatttagcct
     9841 tttaacagct attggatatt ctgctcttac ataaatatat ccttgattag atccaattgc
     9901 ataacctgca atagccattg cttcaagtac gctatgagga tctccttcaa gaactgacct
     9961 atccataaaa gctcctggat ctccctcatc agcattacaa caaacatatt tttggtcatt
    10021 ttgagcagca gcagcaaatt tccattttaa acctgtcggg aaacctgctc cgcctcttcc
    10081 tcttaaacct gaatccagta ataattgaat aacttcctga ggtttcattt cggttaaaac
    10141 tttaccaagt gcgctataac cgtcattttg aatatattca tcaatatgct caggatttat
    10201 attaccgcaa tttcttaatg ctattcttat ttgctttttg aatgatcttg tttcaagcac
    10261 atttttaatg catccattag gttcatcaca tataaggtaa tctgtacaag gtttaccttc
    10321 aattatatgg ctctgcataa cttcagcagc cttttttgaa tccatgtgta tatatgtgaa
    10381 tctatttcct tttgagtcat atacttctac taatggctct aaacgacata taccgataca
    10441 tccagtaagt tgaatattaa cattatctaa tttcttttct tgagcaattt ttttgaattc
    10501 agacattact ggatttgcac cagctgcaat tccgcaagta gccattccga ttaatatttt
    10561 ataatcgtat gctttaatgt ctttttcttt aattgctttt aattcagcta atgatttcat
    10621 taattacctc cgttaaccgc ttaatatttt ttaaggatat tagcgacatc acctggcact
    10681 aatgttccat aaacatcttc attaatcatc acaacaggtg ctagtccaca agctcctaca
    10741 catctgcatg catcaagtga aaatttacca tcttctgttg attggcctac ttcaataccc
    10801 aattgttttt tgaattcctc aaggatatta tttgaacctt ttacatagca agctgttcct
    10861 aaacaaactt gaattttata ttgtcctttt ggatataaag agaactgagt ataaaatgtt
    10921 acaactccat atacttcaga caaaggaaga ccaagtgcat ttgcaataac ctcttgtaca
    10981 gataaaggaa gatatccgta tatttcctga gcttcatgaa gtactggtat cagagcacct
    11041 tttgaatctt tgtgtttatt tagtacttcc tgaagtttca tttctttttc gtccagatta
    11101 caattctgac acattaaatt tacctccgac atagttttgg tatttaacct tagccattat
    11161 atcacagtac attgttattt caataacaaa gattgggttt tatgttaatt ttttaacatt
    11221 tatttattat tcaatagatt tttactatat ttttaataat taaaaagttt gccttgaaat
    11281 atggtatagt tatatttaaa gtttttctcc aaaatattaa tgtctgcatc ataaccttct
    11341 tttaaaaaac ccttattttt aagactcata acatttgcag gatttgaagt tgcacttttt
    11401 aatactgaac ttttattaaa accattatta atcattctga acacaccttc tgccattgta
    11461 atgctactgc cggcaatagt acctgattta tcagttacta acccattatg aacacttaca
    11521 gataatcccc caagagaata ttgtccatct tctaggcatg ttgcagatat tgagtcagtt
    11581 accataatta gattgtcttc aggtttgcat ttaaataata tatttaacat ctctttacta
    11641 acatgataaa aatcaggtat atactcacat tttacattgt catttaaaag tgatgttcca
    11701 gtaatagata catccctgtg atgcatcaat ggcatagcat taaaatgatg agttgataat
    11761 ctatatccat tttcaaaggc cttgtatgaa tcttctgcat tacagatagt gtggccaaag
    11821 gatacaacaa ccccattttt aataagatat tttgctaata aataattttt atcaagttca
    11881 catgctattg tgactcgttt tacaatatca tgatatttcc ctactacttg tttatatgaa
    11941 ctaactgatg gttctataac aaactctttt ttatgagcac caagtttact tatgttcaaa
    12001 tatggtcctt caagatgtac tcctctgaaa acagcatgat taacaatgct gaaacgtttt
    12061 aataatttaa taatatcttc taaactcatt gtaacagttg ttaaataaac ggatgttgtt
    12121 ccttttgaaa catagaaatt aaacaggtca tctgtgttgt aattgtatga agttgaaaaa
    12181 tctataccat ttattccatg agtatgtata tcaatatacc ctggtacagc gaaatttcca
    12241 tcaatattta ctatttcttc gtcttcaatg ttttttatat tcttaccaat tccaataatt
    12301 ttattatttt taattaagat atcatttaat ccttctaaaa attttaaacc gttaaaaatt
    12361 ctacaatttt ttataatcat aatttatctc ctataatcct gtttttgaaa gattattaat
    12421 actatcttta attaatcttt catataaata aatttttccc aattcatctt tcacagcttt
    12481 ttgcgataat ttaagtaaat ccatatcctc atatatatta gcaattttga aatctggaag
    12541 tccatgttgt ttcagtccaa agaaatctcc aggtcctctt agcaaaagat cctgttgaga
    12601 aatatagaaa ccatcattcg aatttaccat tgcattcatt cttttttttg aaatatcact
    12661 ttctgactga ttaaaaagaa cacaataaga ttgtttatca gatctaccta ctcttcctct
    12721 tagttgatga agctgagata agccaaattg ctgagcatct tcaacaatta tcaatgttgc
    12781 attaggaata tcaataccga cttcaataat acttgtagtt acaagtactt ttatatcatt
    12841 atttaaatat ttgttaataa cttcattctt ttgctggtta ttcatttccc catgaagcat
    12901 tcctattgat attcctggaa gatcctccaa taatttttta tgtttttctg ttacatttgt
    12961 aatatcaatg tcattatttt catatattgc cggacacact acgaaaactt gctctccaag
    13021 cgaaacaatc tttagtatga agtcatttat tcgttttcta tatgtttcat caactgaata
    13081 tgtaagcacc ttctttctgt tctttggtag ttcagtaatt gatgttacat caagatcgcc
    13141 atatataata agtgcaagtg tccttggtat tggagttgca gacataacta ataaatgagg
    13201 cttatcacct ttacttgata aaatagatct ttgagcaact ccaaatcgat gttgttcgtc
    13261 tgttatacat aaacccaaat ttttaaaaac aacatcgtcc tgtatcaaag catgagtacc
    13321 aattacaaaa tccgcttctc catttactat tttatttaga gtttcttctt tctctgcttt
    13381 tttcatgctt ccagttaaaa gatatgtcct tatccctaaa ccttgcagca agctttgtgc
    13441 tgtttgataa tgctgcattg caagtatttc tgtaggtgcc atataagcgc tctgatagct
    13501 gttggaaaag gccgacatta cacaataaaa tgcaacaaca gttttaccgc ttccaacatc
    13561 accctgtaaa agcctgttca ttggatatcc gcttaataaa tcatttttta attctgttat
    13621 tgcctgcttt tgagaatttg taagggagaa aggaagtttt gaaacaaatt catctgcagc
    13681 tttatatttc aaaaaaggaa tttttgtctt ttcattttta atattttgtt ttaatgaaaa
    13741 taatgctaac tgcataatga aaaattcttc aaaaaccaat cttcttctcg ctaattgaaa
    13801 atcttcagat gaatttggaa aatgaatatt ttttattgcg tattttttct cacataagct
    13861 gtatttcttt cgtaaatcaa ttgaaaattc gtctactatt aaattatcaa tatattgaaa
    13921 tgcagatttt actgattttc ttatatcata ttgtgttgtg tatctatttt tgggataaat
    13981 aggctgaata gtaaaaaaat cagattcatt aaaagaaaaa tctgggttgt tcatttttaa
    14041 gaaaaatccc ttcttttcaa tttttccata aattaaaata gttttatctt ctttcagaat
    14101 atttttcaga taaggttgat taaaccaagt taattctatt tgtcccgttt tgtctctaaa
    14161 aaaagctgtt gtgatgtgta attttctacc atatggtaac ttttttgaaa taatattcgt
    14221 aataatacct tttaatgcga tatattctga ttcattagca ttagaaacat ttgagattgt
    14281 agatctatct atatatgatc ttggataata ctcaagtaaa tcttttatcg aagaaattcc
    14341 taaactattt aatttttcat ttgctttagg ccctatacct ttaacacttg ttaattcgtt
    14401 catggctgat tttccaactt gttaactaat ttttctaaaa ttacagagca ggagtcatag
    14461 aaaatataat cagctctatc atcatgtaat gtagttgttt tatttataat acataaacaa
    14521 tcttttttat aaaaatcaat gaatgatgca gcaggatata cagataagct tgtccctgcg
    14581 actatcatta catcagtttt taaaatttta gatattgaat tttcgataac tgattcatca
    14641 agctgttcct catatagtac gacatctggt tttacaagac tgttacaata atcacattta
    14701 actatgttgt tagtattttt tatcatgtag tctaaattaa aaaatttttt acaagatgta
    14761 caataatttc tgtttatgct accatgtagc tcataaacgt tagtattttc tgcttcattg
    14821 tgaagattat caatattctg ggttattaca gcaacatcgt aacccatttt ttcaagctta
    14881 gtaatatttt tatggaattt atttggtttt gcatctatat gaactatatt atcaaaataa
    14941 aatttataaa agatatctgt atgctcataa aaaaaattat ggcttaaaat atgctcagga
    15001 ctataacctt ggtatctttc tttatataat cctttactac ttctaaaatc aggtataccg
    15061 ctttctgttg acactcctgc tccggtaaag aaaacaattg attttgaatt tttaattttt
    15121 tctactagat tatcaatttt catcttacct ctattatgta tgacaatata tctgttttat
    15181 taggacttaa tgatttcaca ccaattttgt tttcaatttt tttgtcaata taattacagt
    15241 caggaagact tgaccatggt tctatacaca catagtttga ataagtaggt cttgaccata
    15301 ttccaagata atcaaaatca tcaaaattta cagttatact tctatcagaa ccatcacatt
    15361 tcaaagttac cgatcttgtg ttaagttttg aacaatctaa tattaatgcg tcattagtga
    15421 atatttgagg attcaggtaa agttttttat tattcaaaat tatttttttt aaatctttat
    15481 taagcattat atctttatct gttgtataag agtaaatatc attatcaaaa aattcaatgt
    15541 agtaatcatc cattacttta ttttcagaaa aagttaaatt aaaaccctca tgtcctccaa
    15601 cttcaaaata cataggttta taacctttgt ttatgataat atactgtttt ttaattgatt
    15661 cattcattaa ctcatatgta ataattaaat caaaatcaaa aggatatgaa gaatatgttt
    15721 cggtgtttgc tgaaagcata aaagaaacat aattttcagt attttcagta atatcaaaca
    15781 ttgaatgttt tacaaatcca tgtattttaa tattatacat tcttccctca tgttcgtatt
    15841 cttcatcttt aagtctgcca ataataggaa ataaattggg agcctgtcca ttccatatat
    15901 ttggattctt ttgccacaag tattcatatc catttttttt gattgatact atttctgctc
    15961 caataggatt tatctcaaca cttatctctc tattttttaa ttttaccata atacaattac
    16021 ctcaaaaaca ttataccata atcattattg aagttttata tttaatatgc tatattaaca
    16081 aaaggaggat tatataaatg aaacatacaa ttgatataaa atttctttca ataagtgatg
    16141 ttaaagaatt tgcagatatt gtttcagcat ttaattatga tatagatttg atttcaggaa
    16201 gatatgttgt tgatgcaaag tcaataatgg gtatcttcag tctggatctt agcaaaccaa
    16261 tttcattggt tgctcatact gataatgtct tgcctttggc agaagctata aagaaatttt
    16321 gctaaaaata gaattgaata tttattaaaa taaaacggga ttaaacccgt ttttatttgt
    16381 aaatttaaat tactcttttg attatctaat tagtgaacag ttaaatgatt atatttgttt
    16441 attaattaat tcatttatag tgttcgacaa atatgaattg tcagttaatt ctatcatacc
    16501 tctgtcaatt gcagaactta attttttatc aaaaggtatt ctgtctaaaa taggtatact
    16561 taaattattt ccactttcat ttccaaatag ttcaatttta tttccacatt tatcacatat
    16621 tacataactg aaattttgaa ctgctcctaa aaccgaaata ttcatcatgt cagccatctt
    16681 aatagctttt tcaactatca tggaaaccaa atcctgtggt gatgtgacta ttaaagcata
    16741 atctactggt aatgattgaa agattgttaa tgcagtatct gctgttcccg gaggcatatc
    16801 aatcagtaga tagtccaatt ctccccatat tacatcagta aaaaattgct ttaatacacc
    16861 cgatataata ggtcctctcc ataaaaccgg atcagtattg ttttcaagta aaagattaat
    16921 actaattatt ttagttccta aagaggattt tgctggaaaa atacctgatt cgttacccat
    16981 tactttatca tttatattaa acgacttagg tattgagggt cctgttatat ctgcatcaag
    17041 tattcctact ttatatccaa gccttgttag ctcaacagct aaaagacttg taaccatact
    17101 tttacctact ccgccttttc cgctaactac agcaaccaca ttttttattt gtgaaaaatc
    17161 atttgctttt tctttatcaa ttctgctttc acagttatcc tgagaacaac tttcacaatt
    17221 atgattacat tctgtcattt atttctctcc tattttatgt agtatattga ttggtcagga
    17281 agttttccac caacgcttga tggattgttt tcaaaaagaa attcaaaaaa tgcatcaaga
    17341 ctgtcttttg cgtcagcagc atctatgtat actatattac aatttggtat agctttttct
    17401 gcaacagttt cattatcaag tattcctgat gtaactataa gttctgaagc ctcgtatggt
    17461 ttctctagta cccattctat tgattcttta tataaatcca ggaatgtatt tacctcttct
    17521 ttgtattctt caataaatgt tttttgtgct accaagcatc ccatggatag agtaatatca
    17581 ggagcttttt cagtccattc ttcattaaga tcaattgcat gtattatgct ttcatcttta
    17641 ttcattactg ttgaaacaaa cggctcagga agcatgacta tatcaacttc tccagataac
    17701 aatagagtag ctacttcact gtgttctgct ttgtagatta tctcaacatc agtttgaggc
    17761 actattccat tttcattaag aataaattct aaaacatatt gtggtaaaga accttgtccg
    17821 cttgctgtaa ttgttttacc ctttaagtcc tctattgagt tgattgttcc tgttttatca
    17881 aggatatgta aaactcctaa tgtatgtact gcaagtatct gaatctctcc ttctgttctg
    17941 ttgtataaaa cagaagcgag attagttggt actgcagcaa ttgttatgct tttatcaagt
    18001 aatccagcta caacagtatc tgttgatgtc ataatttcaa aatcagtatt tacataatca
    18061 gataaagata ctatttcatt cattaccctt accattccta tacctgtagg acccttcatt
    18121 gcgacaattt ttgctgtaat cgggtcttct ggaggatttg tttcataaat cttattacat
    18181 cctgctaaag atatcatcgc aataatagca agtactatgc atattatagt tttagatgtt
    18241 ctttttttca ttttcaatac ttcctttcgt taaaacctac actatattat atccttgaat
    18301 aaattgttta caagaatgtt ttttttaatt cttcaatatt taaagttttt atacctagtt
    18361 tatctgcttt atcaatttta cttccaggat tttcgccaac tataacataa tttgtttttt
    18421 ttgaaacact atcagttaca ttagctccaa aactagtaat tagagatttt aagtcctctc
    18481 ttgaataatc ttcaaatgaa ccggttataa caaatgtaat accttttaac ctgctattac
    18541 ctgatttgcc ataattaaga gagttcatat taataccttt ttcttttaaa ttattaataa
    18601 gtatgaccgc ttcaggattt ctgaaatatt gaactatgct ttttgccatt ataaaaccga
    18661 tagtatcaat ttcagtaaaa gataattcgt ctgcagtcat aacggaattt atatctttga
    18721 atttatctgc tattaatgat gaagcttttt ttcctatatg ttcaatacca agaccaaata
    18781 ttagtctgtc tatattatta tttttagatt tttcaattgc gtttagcaac ttatcaatac
    18841 ttttagttcc aaacccacta attttaatta attctttcct gtttgaatgt aaattgtaaa
    18901 tatcatctat agttcttatg aatccttcat tcataaattt atcaataata cctgtactca
    18961 gtccttcaat atccatagca tcttttgagc agaaatgttc aagttttcta gatatttgcc
    19021 caatgcaatt tatacctatg catttatatg ctgatttatc ttccattttt actactttag
    19081 aattacataa cggacatttt tctggcatgt taaaaataat ttcatttccg gttctttttt
    19141 ctttcaatac cttaactacc tcaggtatta catcaccagc tttttgtatt attaccctat
    19201 cattaatcct tatatcattt tcccttataa aatcaaggtt gtgtaatgtt gctttactta
    19261 ttctgctacc agatacaacc acaggatcaa aaattgcatt aggagttaat actccggtgc
    19321 gtccaacttg tacaaatata tctttaacta atgtttcttt ctgttcagga ggatatttgt
    19381 atgctattgc ccaacgtggt gcttttgctg ttgatccaag tacctttcta tcctcaagat
    19441 tatttaattt tataacagca ccatcaatat cgtgtttata tgtatgcctt tcaatttgaa
    19501 ttttttctat accttttata atctcctcaa gatttttaga tataactatt atttcatttg
    19561 tttcaaaacc tatttttcgc aaaaaagtaa gtgcttcaga atgtgtttta aaaatagttc
    19621 cttgaattgc ttgtatatta aaaataaata ttttaagttt tctcttggct gttattgaag
    19681 gatctaattg tctaagagac cctgctgctg catttcttgg attagcaaat tccttatcac
    19741 ctgtctcaac tgctttttca tttatctttt tgaacgcatc cttatccata tatacttccc
    19801 cacgtacttc tatatatgga ataatttcat tatgtgaaag ccttaatggt atagatggta
    19861 ttgtttttag attatttgtt atattttctc caaatacacc atctcctctt gttgaacctc
    19921 ttacgaatat attattctca tattccaaag atactgacaa cccgtctatt ttcttttcta
    19981 aaacatattc aggttcaata ccttctttta aaattctctg attaaaagca tataaatcat
    20041 catatgaaaa tgcatttgac aaactttcca taggtactat gtgttcaact ttatcaaatg
    20101 atttcagagg ttgacctcct attcttttag tcggtgaatt tatcgaaaca tactcagggt
    20161 atttttcttc taattttatc aactcattaa gcatcatatc atagtcaaaa tcggatattt
    20221 cgggagaatc ttcttcatga tatttttttg tatgataatt taataactct actaattcat
    20281 ccattcgttt tttcataaat atattttacc atagttatta gtgtagtcta tataaattga
    20341 ttattttctg tattagtgtt aatattatga gtgaatttct agtggaggta aatattttgg
    20401 ctaattcaaa atcagcatta aaaagagttc gtgtctcttc taaaaagaaa ctacaaaatt
    20461 cttcaaaaag atcatcattg agaaagacaa tgaaaagagc taatactgta atcgaaaatg
    20521 aagaaaagga taatgaagcc atattaaaag ctgttaaagc agtagatcag gctgtatcaa
    20581 atggaattgt tcatagaaac aaaggagcaa gagacagaag taaattaatt aaagctttgt
    20641 ataagtaaaa taatgtatat tgttaatacc ggaacaaaca ccgggttttt atatgcttaa
    20701 aaattgtaat ttgtgtccaa gaaattgtaa tatagataga gcatctcaaa aaggttactg
    20761 tagagcacct gataatatta tgttagcaaa ggcctctact tttttttatg aggaacctgt
    20821 agtaagtggt aaaaatggta caggagctat tttttttagt ggttgtaccc taaaatgctg
    20881 tttttgtcaa aataaagaga ttagccaaga tatgaaaggt ttgactgtat cagatactca
    20941 gttttcagat atacttctaa atcttcaaga taaaggtgta gaatcaataa gtcttatatc
    21001 tgctactcca tatgtactta atattatacg ttctattga
//
LOCUS       DAOSXM010000032        20700 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2111
            NODE_1620_length_20700_cov_12.026738, whole genome shotgun
            sequence.
ACCESSION   DAOSXM010000032 DAOSXM010000000
VERSION     DAOSXM010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864178
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 20700)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20700)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:24:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,261
            CDSs (total)                      :: 2,229
            Genes (coding)                    :: 2,218
            CDSs (with protein)               :: 2,218
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 3 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20700
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1620_length_20700_cov_12.026738"
                     /isolate="AMR_MDS_2111"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            109..909
                     /locus_tag="PLQ68_05760"
     CDS             109..909
                     /locus_tag="PLQ68_05760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72190.1"
                     /translation="MRRKIVVLVAAVLALVFIMGLASCTPYDKRPKPSINTENALEDR
                     YIVKEKEYTIATYGGDPVIKPTVSQFNNISRQKTLNARLEAFNEMVGMNPETARNEQL
                     YRSYEIHQKNYYMFSCQYFTAFETTVSNKGVNIIIGSEIDGTLMMNFPALLGNNIHSD
                     GWEEFFIFFNEAREEAGAKEIEMEYMTTDNVSFVFVGEDLSDLTLLVMYKAPDDTEQQ
                     EVPMSLNKIKSTFTERMQIYLSFTDEADMPYEQYALAITEYKQMMAEE"
     gene            1008..2324
                     /gene="rsxC"
                     /locus_tag="PLQ68_05765"
     CDS             1008..2324
                     /gene="rsxC"
                     /locus_tag="PLQ68_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014253461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HXK72191.1"
                     /translation="MIKQFKKIKGGVILPHNKGTSEIPSKIMPIPELVEIPMQQHIGA
                     PCETLVKKGDKVYVGQIIGDSKQFVSVPIHSSVSGEVTETRKTMLFNGTVSESVVIKA
                     DGLQTILPEISKPDISNKENFLKAVRNCGLTGLGGAGFPAHVKLNTKDRIPEYLIVNA
                     VECEPYITCDYRAIIEDGEEIIKGIKLILDNLSIPKAYIGIEDNKCSGAEKLEKLASE
                     DDRIDVVVLKTKYPQGAEKMLIYALTGKKVPMGGLPIDVGTVVMNVTSVQTLARYCET
                     GIPLISRRMTIDGKGVKERGNYHIVLGTYIKDIFEFCKGDKEKTLKILCGGPMMGMAA
                     KDLDMPVIKQNNALLFMTQEDLDFIEESACIRCGRCIDACPMGLMPLTLNKYALAKMT
                     DELEKYNLMNCIECGACSYVCPAKRHLVQSFRIGKELLKKEKVKNG"
     gene            2317..3237
                     /locus_tag="PLQ68_05770"
     CDS             2317..3237
                     /locus_tag="PLQ68_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HXK72192.1"
                     /translation="MDKFIVSSSPHIKDRSRTYRIMLDVIIALMPTAILSVYYFSYVA
                     ALIIVTSVSSCVLFEYLFNLIAKKKHSETDLSCVVTGLILALNMPPLKSSILLSIIGA
                     FIAIVVVKMLFGGIGQNIFNPAAAARIFLTASFSSLMTVYVAPFSDGVTSATPLVLVN
                     NGNMPTYLDMFLGKTAGSLGETAAVTLIAGGIYLMIRKVISWEIPVTYIGTVALLSFV
                     FGQDLLFSILAGGLMIGAIYMATDYTTSPMTRSGKFIFAAGLGLMTASIRRFGSNTEG
                     VAYSLLFMNALVPLIDRFTTVKTFGKVKKK"
     gene            3234..3767
                     /locus_tag="PLQ68_05775"
     CDS             3234..3767
                     /locus_tag="PLQ68_05775"
                     /inference="COORDINATES: protein motif:HMM:NF016121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HXK72193.1"
                     /translation="MKKYFIPVLSLFLITACVVGLLALTNHFTMGIIQERERCAAEEA
                     LEIVLPGGAPYTNITDSIEDMSIDIEAKFTCANQSDKGYVFTMVSKGYGGDIIVYVGI
                     NNEGKVVNIMLGDNEETPNLGKKAELPEFTSQFSGLSKEDEIPDKIDYITRATITTDA
                     VIVAVESALEIYSEVTK"
     gene            3764..4423
                     /locus_tag="PLQ68_05780"
     CDS             3764..4423
                     /locus_tag="PLQ68_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010246511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HXK72194.1"
                     /translation="MKNRSLQEFSKGILKENPTFILVLGMCPTLAVSTTAENSFMMGL
                     AATIVLVLSNVIISLLRKLIPEKVRIPAYITVIAGFVTIVQLLMKAYLPELNKALGIY
                     IPLIVVNCIILARAEAFAAKNNPWYSLLDGLGMGLGFTLAITLIGIIREFLGSGAVFG
                     LQIIKNIPMPSVFVMPAGAFLVLGMLMALISALTHKKTEIKDCSECPMQCLPKKEKGD
                     N"
     gene            4423..5016
                     /gene="rsxA"
                     /locus_tag="PLQ68_05785"
     CDS             4423..5016
                     /gene="rsxA"
                     /locus_tag="PLQ68_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015360063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HXK72195.1"
                     /translation="MNGDTFKLIIYISFGTILMNNFVLTNFLGICPFLGVSKKLNSAV
                     GMGLAVIFVMTIASIVTNLTNRYLLVPNGLEYLQTLAFILVIAGFVQLIELFMKKVTP
                     PLYKTLGIYLPLITTNCAILGVALLSVQNEYNLWQSAIYGASAGIGFLLATVIFAGIR
                     ERLDLNDVPKYFKGLPIALITAAFLSMAFLGFKGIFG"
     gene            5025..5819
                     /locus_tag="PLQ68_05790"
     CDS             5025..5819
                     /locus_tag="PLQ68_05790"
                     /inference="COORDINATES: protein motif:HMM:TIGR01944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HXK72196.1"
                     /translation="MLKEILIPIAILGGIGLFFGIALTIASKVFEVKKNEKAEKIRQI
                     LPGANCGACGYSGCDLYAQAVAEGKAEANLCIPGTNTTAMAIGEVLGLKVEKQKTRVM
                     KALCCPDVTKKFEYKGIQTCAAAALLYKGENSCNYSCLGFGDCVAVCPHDAIMMKDGI
                     PIIDPMLCTECGLCAKACPKNLIQYLIGECGYYVACVSQDTGKVTNKNCKNGCIACGL
                     CVKACQDDAIHVINNVAIIDYEKCTACGKCYEVCPKKIIKKFCSSY"
     gene            5869..6291
                     /locus_tag="PLQ68_05795"
     CDS             5869..6291
                     /locus_tag="PLQ68_05795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72197.1"
                     /translation="MAEDKKVVNIEDKEKALKKMRMERIITISVLAILVLGIISALIF
                     KSIVGTWWVEVQEDSTTTGAYIYFGGDKTYKTFMYGVDQWSETGTYEIKGTSVNGYIL
                     FNPENGQQYIRRYNIRGTRLKIFNPDDTYISFLRNQSN"
     gene            6416..8905
                     /gene="gyrA"
                     /locus_tag="PLQ68_05800"
     CDS             6416..8905
                     /gene="gyrA"
                     /locus_tag="PLQ68_05800"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004623406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HXK72198.1"
                     /translation="MENEKINDVSKIIPVDIEEQMRKAFIEYAMSVIADRALPDVRDG
                     FKPVHRRILYAMHSLGFYPEKSHRKCAATVGEVLGKFHPHGDAAVYDAMVRMAQDFSL
                     RYPLVDGHGNFGSRDGDPPAAMRYTESRLTKIAVEMLADINKETVDFKSNFDDHEMEP
                     VVLPSRFPNLMVNGSSGIAVGMATNIPTHNLVEVIDGIAAVIKNPDITIDELIEIIKG
                     PDFPTYGTIMGRSGIYQAYKTGKGRVVVRGKTEIEEAANGKQRIIIHDLPYQVNKAKL
                     IETVASLVKNKVIEGISEINDESDRNEEIRIIITLKKDANANVILNRLYKHTQLQDAF
                     NVNMLALTPSKMGYEPKILNLKQMLEYYILHQVDVIKRRTKFDLDKAQTRAHVLEGLI
                     KAVDILDAVIETIRGSSNEPEAKENLVSKFDFSDRQAQAIVDMRLGRLTGLEILKLQS
                     EYDDLLEKIKYLTNVLINDEVTHQVIIEELEVIKKKYGDERRTRIEESEEDLLDEDLI
                     KEETVVLTLTRFGYIKRLPTDTYKSQNRGGKGVIGVTTREEDTIIDIFTTSTHSYLLF
                     FTNRGRIYKLKGYQIPEASRTAKGTAIVNLLELEPNEKVTTVLPIESFETTQNLLMAT
                     KLGFAKKTPLSSYTNIRKGGIIAVTLRDDDDLISVKLSEGNDTVMLITYKGKAIKFTE
                     KDTRETGRSAKGVIGIRLDDDDYVVGMDLVKDEETVLMITENGFGKRTFISEYPIQKR
                     GGKGVITYKVTERTGPVISSMIASDEDDILLMSLQGDMIRLDASQISILSRATQGVTL
                     MRIDEENMVVAAAKVEKEEELEEDQEDSKKE"
     gene            9268..9612
                     /locus_tag="PLQ68_05805"
     CDS             9268..9612
                     /locus_tag="PLQ68_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017751824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HXK72199.1"
                     /translation="MIIASDLIRGNTDTIILASLMKKDNYGYEINKNISIKTDGKYEF
                     KEATLYTAFKRLEEAGFIRSYWGQENVSARRRYYSITPKGKDEYHKLLEDWKNAKNMI
                     EKLIDTGVVDNG"
     gene            9605..10231
                     /locus_tag="PLQ68_05810"
     CDS             9605..10231
                     /locus_tag="PLQ68_05810"
                     /inference="COORDINATES: protein motif:HMM:NF038403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease prefix domain 1-containing protein"
                     /protein_id="HXK72200.1"
                     /translation="MDNKLKAYVDNLFKNAPATAKAYEVKEEILQNLKDKYNDLIAEG
                     KSENEAYKIAIESIGDLTELFIELEKETVPDNSKQEKYMALKAVTAMIFVLALIPLVM
                     SFSGRAVLFNLLAVVILIAIGIILSVYNSSNKPGYKYYGGQAYKMAEIAKKNNIRKSV
                     SGFLWLITIVLYFIVSFQTHAWHLTWLMFLLAAAIECILNIWLGQGKV"
     gene            10241..11335
                     /locus_tag="PLQ68_05815"
     CDS             10241..11335
                     /locus_tag="PLQ68_05815"
                     /inference="COORDINATES: protein motif:HMM:NF024743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HXK72201.1"
                     /translation="MSSTSSKIIRTIAILVIIFVIIGLGIWYVAEYGFKGISFSTTGR
                     HTIQTLTGPFEEKARYEADSDDIEEIQVNWITSAVDFKVHDKDTILFVESCQRDLSEG
                     ENLTYTIKDGILKITELEDETIVRIAPKKVVIWIPESLLETLSVLRSNCALADVTAED
                     ISSDTIYLESDTGRINVKNLTANQTLTLNTDTASITASDIKAKNIVIDSDTGNIDVSN
                     LQADVKIDIETDTGRVEVNDVTTETLICDSDTGRVICLNYDVDSITQSSDTGALETEG
                     IFKTGDFRSNTGSITMKCATLPDEIYAKSDTGSIYLYTVKTDNLIVAYSTATGKFHSD
                     FPVLVSSSGDANITMKTATGSIYIRPYENE"
     gene            complement(11427..12326)
                     /locus_tag="PLQ68_05820"
     CDS             complement(11427..12326)
                     /locus_tag="PLQ68_05820"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HXK72202.1"
                     /translation="MEFLKALEQIRTPFFDKFFLLVTKMGEELFFTVIILAVIWCIDK
                     KIGYRMFFIFTLGSFVNQLLKLIFKVPRPWTYENAAKPVEGSVEAATGYSFPSGHTQQ
                     GMSLFGSIAMFMKKSWVYIVMAILALLVAFSRMYLGVHTPADVFTSLAIGIALLVLCY
                     FVFRIADKYKYGDAYLYGIMAVAITIAIAVLSFDKTGSVEYLHGIENGWKLLGCCIGI
                     IISVIVDIKYTHFEVKAKWWQQVIKLVVGLGIVIVIKTFLKQPLLDLFNQNPIADGIR
                     YLLIIVFAGCLYPMLFKLFKKKV"
     gene            complement(12428..12793)
                     /locus_tag="PLQ68_05825"
     CDS             complement(12428..12793)
                     /locus_tag="PLQ68_05825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72203.1"
                     /translation="MNRYGMDTFNRALIIFYIIFLFLSLITRNDIFMYISLALAIYQV
                     FRAFSKNFAARSKENQQFLKLWNPVKRKYYSIKKKLSDKTHRYYKCPKCKTEIRVPKN
                     KGKIRITCPKCKEQFIKNT"
     gene            complement(12922..13632)
                     /gene="fabG"
                     /locus_tag="PLQ68_05830"
     CDS             complement(12922..13632)
                     /gene="fabG"
                     /locus_tag="PLQ68_05830"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020815915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HXK72204.1"
                     /translation="MRTVLITGASRGIGQQAAFDFLEAGYNVVVNYNQTYINEADYLP
                     YKDHVIFIKADIADYSQVQKMFVDASNQFGNIDVVINNAAVTYLGLLSQMDEKDWDRL
                     MDINLKGLFNVCKCAIPSMVNNKKGHIINISSMWGITGASCEVAYSASKAAVIGFTKA
                     LAKELAPSNILVNCIAPGIINTDMNKKFTKDELEEMIPLGKIGNCKDITDMMLYLANE
                     KTSFITGQVFSINGGSVI"
     gene            13838..16201
                     /locus_tag="PLQ68_05835"
     CDS             13838..16201
                     /locus_tag="PLQ68_05835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72205.1"
                     /translation="MKRHIVSITVLIVVLAFCVAVLNGQVNTSHDVVTHEMRYQTNEA
                     EVEISDQQTVVKGAWDNSLRIYNDRTYSLIFNNSFTEKWYVLDVSDIGKSLSFLISCD
                     DESVTGIIASLYLKEDIDEYGNNATRLMNLYSLNKSATYKIKETGTYYIKISIIGNSA
                     VGKELNFGYTLVINDSYEDNDTWQKAVNITQGEEYDIDISAANDTDWFKITLPKNDQS
                     YQYILKNEIATSSKAQVFVYKEEDLKKYDDSAQNTFYSDEFNKYFTYKSEESTTYYLK
                     IVPPVQGSIMNKITFIADIIEPDAMENNDVFDKAVMLEPDMSKTLTISASNDIDWFMI
                     KTDKPGQTVRLSLINPSTAAQKLNCWWYSNEKIMTYRETASPEYKSSGSGYTYIYLEE
                     ASVYYLKIFTEDKSVIKTPIDLSYSIIGADKNEPNNSYSEAHNITSLRETSFTLPALN
                     DEDWFAIDIAKSNQAIRIDTSISKTGASVDVSFYSLNDMQSYGEAIPLFSFNSKHAPY
                     IYLLNDPGKYYMKITSDTIISDECTLYYSLIDSDRNEPNNSAEKATILEQGVKTGFTL
                     SGQNDEDWFEIEIQNDGDVLEYIIEAKYNRYAEVQIYSKDELNKYDEYANPLFTSSIY
                     DESITCKFEKAGAYYIKLSSDKPILDITYITYSLSNSDAYENNDTWQDAVLIERNKDI
                     KFTISASNDVDWFKMENKGMNNMSFSYKIPNTFMAKNIIGVFVYREIDLLNYGLRAEP
                     IYSVKTADLSNMFEIEEGTYYFKIVSLSDVAFEYEFTFKANMFYMQQ"
     gene            16255..18057
                     /locus_tag="PLQ68_05840"
     CDS             16255..18057
                     /locus_tag="PLQ68_05840"
                     /inference="COORDINATES: protein motif:HMM:NF019556.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HXK72206.1"
                     /translation="MHIETKHINIKEGFWYKRQNINKQVTIKAVQNRFLETGRFDALD
                     FDNEKIPHIFYDSDVAKWIEGVSFTLYKERNAELETFIDHLIDLIEKNRSEDGYFNSH
                     FLRKPEERWKNREDHELYCAGHLMEAAVEYYKATGKDKFLKLMQDYADYIYKVFVIED
                     SAAFKTPGHEEIELALVKLYDVTENKKYLELSKYFIDTRGTIEEPYILEKYNHYNIQD
                     HLPVRQQKTAEGHSVRASYLYSGMADIAVKYNDTELFEACKTIFSNIVNKRMYITGGT
                     GSSHLGEAFTLDYDLPNSKGYAETCAAIALAFFAQRMLLLEKNSVYADTVERVMYNGM
                     LSGISLDGKAFFYENPLEIQPVLNKRNVSTTFTERNPITQRKEVFSCSCCPPNVIRFI
                     GAINGYIYSYEQDILFINQYISSEMLYENIQADMKTDFPSSGNISIKTKNINQIAIRK
                     PYWCTDVDIKVNGNDAIYTLKKGYMYLGLTETNNTIDIKMDMPVRIVRSNPKVHEDSK
                     KTAVMRGPIVYCAEGIDNDFPLNQILFNEKCEYKVNKSNDLDADILKLRVKIPKEIDE
                     LYTYKPFEYIDKELTLIPFYCFANRGETEMRVWL"
     gene            complement(18297..20609)
                     /locus_tag="PLQ68_05845"
     CDS             complement(18297..20609)
                     /locus_tag="PLQ68_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014520131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXK72207.1"
                     /translation="MLRLKDIKKSYIVGDFKQNALNGVSVSFRKNEFVAILGPSGSGK
                     TTLLNIIGGLDRYDTGDLVINGKSTKNFKDSEWDAYRNNSVGFVFQSYNLIGHISVVK
                     NIELSLTLSGVSKTERRKKAMDALELVGLKEHAKKKPSQLSGGQMQRVAIARALVNDP
                     DIILADEPTGALDTETSVQILDLIQNIAKDKLVIMVTHNKEIATKYANRIINLRDGEV
                     IDDTNPVTEEPTYKEKYSFKKTAMSFFTALSLSFNNIMTKKGRTILTAFASSIGIIGI
                     ALILALSNGFDKQVQTFEEETMSAFPIMISQSAMNVDYDQLQTMRNQNIFGSSENYPD
                     IKAVYPYDPSQSSMRHTNNITEEYIEYVEKIESSLVSGLQYSRAINFNLMIKKNGDVG
                     ILSSSDIHFSVIPKSLDDNSLSVVETNYDLLHGKYPESMYELVLVVDEKNRVNVDILE
                     ALGLDPNQETISFDDIVGIQFHLVMNDDLYVPFNDHFIVNSDFQAVYDSEQSVTLCIC
                     GVIREKDKATMPILNAGVAYTEDLVSYVMENAKNSDVVQKQQEVDYNVFSILGPASPL
                     AQMDKDRLLQVLGATAVPYAISIYPADFDSKDEILAYLDAYNAGQENPEDEIIYTDLA
                     SMITMLSGNIMDAITYVLIAFSSISLIVSVIMIGIITYISVLERTKEIGVLRALGARK
                     KDITRVFNAETFIIGAFSGFIGIFIARLLILPTNVILKNLSNLDNIAVMNPLHALVLI
                     VISVALTVIGGTIPARMASKRDPVEALRSE"
BASE COUNT         7367 a         3130 c         3887 g         6316 t
ORIGIN      
        1 aatgcaatgt aatgattaaa acagatttac aatcaaatcc ataagttgta taatattcat
       61 actgattatt ttatgtaaaa gtgctctata agggtatggg aggaataaat gagaagaaaa
      121 atagttgtat tagtagctgc cgttcttgca ttagtcttta ttatgggact tgcgtcatgt
      181 actccatatg ataaaagacc aaaaccatca attaacacag aaaatgcatt agaagacaga
      241 tatatcgtaa aagaaaaaga gtataccatt gcaacatatg gtggagatcc agttataaaa
      301 cctactgttt cacagtttaa caacatcagc aggcaaaaaa ctcttaatgc cagacttgaa
      361 gcatttaatg aaatggttgg aatgaacccg gaaacagcaa gaaacgaaca gctttataga
      421 agttatgaaa ttcatcagaa gaattactat atgttctcat gtcagtattt tacagctttt
      481 gagacaaccg tttcaaataa aggggttaat attattatag gttccgaaat agatggaaca
      541 cttatgatga atttcccagc acttcttggt aacaacattc attctgatgg atgggaagaa
      601 ttctttattt tctttaacga agcaagagaa gaggctggtg ctaaagaaat tgaaatggaa
      661 tacatgacta cagataatgt aagttttgtg tttgttggag aagatttatc agacttgaca
      721 ttgcttgtaa tgtataaagc acctgatgat acagagcagc aggaagttcc aatgagtctt
      781 aataaaatta aatcaacttt tactgaaaga atgcagatat atttaagctt tactgatgaa
      841 gcagatatgc catatgaaca atacgccctt gctattacag agtataagca aatgatggca
      901 gaagaataag agattaatag atgataataa aaaggacatg taaatgtcct tttttttctg
      961 actaaaacga tatataatat tattattata actagtgagg gaatcggatg ataaaacaat
     1021 ttaagaaaat aaaaggcggc gttatcttac cccacaataa gggaacttcc gaaattccat
     1081 caaagataat gcctattccc gaacttgtcg aaattccaat gcagcagcat atcggtgctc
     1141 catgcgaaac gttagttaaa aaaggtgata aggtttatgt aggacagata attggtgatt
     1201 caaaacaatt tgtttcagta cctattcatt caagcgtatc aggagaggta actgaaacaa
     1261 gaaaaacaat gttgtttaat ggtactgtat ctgaaagcgt agtaataaag gcagatggat
     1321 tgcagacaat tctgcctgaa atttcgaaac ctgacatatc aaataaagaa aattttttga
     1381 aagcagtaag aaattgtgga ttaacaggac ttggtggtgc tggatttcca gcacatgtta
     1441 aacttaacac aaaagacagg ataccagaat atcttattgt aaatgcagta gagtgtgaac
     1501 cttatataac ttgtgattac agagcgataa ttgaagatgg cgaagagata atcaagggaa
     1561 taaaacttat acttgataat ctttctattc caaaagcata tataggtatt gaagataaca
     1621 agtgttctgg tgcagaaaaa cttgaaaagc ttgcaagtga agatgacaga attgatgttg
     1681 tcgtcttaaa aacaaaatac ccccaaggtg ctgaaaaaat gcttatatat gccttaacag
     1741 gaaaaaaagt tccgatggga ggacttccta ttgatgtagg aactgttgta atgaatgtta
     1801 ctagtgttca gacattagca agatactgtg aaaccggaat acctcttatc tccagaagga
     1861 tgactattga tggaaaaggg gttaaagaaa gaggaaatta tcatatagta ttaggcacat
     1921 atataaaaga catatttgaa ttctgtaagg gggataagga aaagacttta aaaatcctat
     1981 gtggcgggcc aatgatggga atggctgcta aagatcttga tatgccggta ataaaacaga
     2041 ataatgccct tttattcatg acacaggagg atctggactt tattgaagag agcgcatgca
     2101 taagatgcgg aagatgtatt gatgcatgtc ctatgggcct tatgcccctg acattgaaca
     2161 aatatgcgtt agcaaaaatg acagatgagt tagaaaaata taaccttatg aattgtatag
     2221 aatgtggggc atgttcgtat gtctgccctg caaagaggca cttagttcaa tcatttagaa
     2281 taggtaagga attacttaag aaggagaagg tgaaaaatgg ataaattcat agtatcatca
     2341 tcacctcata taaaagaccg ttcaagaaca tataggataa tgcttgatgt aataatagca
     2401 ttaatgccaa cagctatact atctgtttac tatttttcgt atgttgctgc actaataata
     2461 gttacttctg tttcttcttg tgtgcttttt gaatatcttt tcaaccttat agcaaaaaag
     2521 aagcattctg aaacggatct ttcgtgtgtt gtaacgggat tgatattggc tttaaatatg
     2581 cctcccttga aatcatccat attactaagc attattggag catttattgc aatagttgta
     2641 gtcaagatgc tttttggcgg aataggccaa aacatattca atcctgcagc agcagcaaga
     2701 atatttttaa cagcatcatt ttcttcactt atgacagtat atgttgcgcc ttttagtgat
     2761 ggagtaacat cagcaacacc gctggttcta gttaataatg gcaatatgcc cacatatctt
     2821 gatatgtttt taggaaagac tgcaggaagc ttgggagaaa cagctgcagt aacattaata
     2881 gccggtggta tctatcttat gattcgaaaa gtaatatcat gggagattcc tgttacctat
     2941 atcggaacag tagctttact gagttttgtt ttcggacaag atctattatt cagtatacta
     3001 gcaggcggat taatgatagg tgcgatatat atggcgacag actacactac aagtcctatg
     3061 acaagaagcg gtaaattcat ttttgctgca gggttaggtc ttatgactgc aagcataaga
     3121 agattcggtt cgaatactga aggtgtcgca tactcattat tatttatgaa tgctctggta
     3181 cctttaatag acagatttac aacagtaaag acttttggga aggtaaagaa aaaatgaaaa
     3241 aatattttat tcctgtttta agtctttttc ttataaccgc ctgtgttgtc gggttattag
     3301 ctcttaccaa tcattttaca atgggaataa ttcaggaaag agaaagatgc gcagcggaag
     3361 aagccttaga gatagttttg cctggaggag cgccttatac taatataact gattcaattg
     3421 aggatatgtc aattgatata gaagcaaaat tcacctgtgc gaatcaaagt gataaaggat
     3481 atgttttcac tatggtatct aaaggctatg gtggagatat aatagtgtat gtaggtatta
     3541 ataatgaggg taaagtagtt aatatcatgc ttggagataa tgaagaaact cctaaccttg
     3601 gtaaaaaagc ggaactgccc gagttcactt ctcaattttc cgggctttct aaagaagatg
     3661 aaataccaga caaaatagat tatattacca gagcgactat aacaacagat gcagtaattg
     3721 ttgcagttga aagtgctttg gagatatatt cagaggtgac aaaatgaaaa acagatcttt
     3781 gcaggagttt tcaaaaggaa tactaaagga aaatccgaca tttatactgg ttttaggtat
     3841 gtgtcctaca ctggcagtat ctactacagc agagaattcc tttatgatgg gcttggcagc
     3901 aactatagta ctggtgcttt caaatgtcat tatatctctt ttaagaaaac tgattcctga
     3961 aaaagtaaga attccagcat atataacggt tatagcaggc tttgtaacta ttgttcaact
     4021 tttaatgaaa gcatatctgc ctgagttaaa taaggcttta ggtatatata ttccattaat
     4081 cgttgttaac tgcataatat tagccagagc agaagctttt gctgcgaaga ataacccatg
     4141 gtactcatta cttgatggac ttggaatggg attaggtttt acacttgcga ttacgttaat
     4201 aggaataata agggaatttt taggaagtgg cgcagtattt ggattacaga ttattaaaaa
     4261 tattcccatg ccttcagtat ttgttatgcc tgcaggcgcc tttttggtac ttggtatgtt
     4321 aatggcctta ataagtgctc tgactcataa aaaaacagaa atcaaggatt gttcagagtg
     4381 ccctatgcaa tgcttgccta aaaaagagaa gggagataac taatgaatgg tgatactttt
     4441 aaactgataa tatatatatc atttggcact attcttatga ataacttcgt tcttacgaat
     4501 tttttaggaa tttgtccgtt tttaggagtt tcaaaaaaac tcaattctgc tgtaggaatg
     4561 gggttagcgg ttattttcgt tatgactata gcatcaattg taaccaatct cactaatagg
     4621 tatcttttag tgccaaatgg attagagtat ctgcagacat tagcctttat tcttgtcata
     4681 gcaggttttg ttcaactgat tgaattattt atgaaaaagg ttactccacc attgtataag
     4741 acactgggaa tttatctgcc attgataaca acaaattgtg caatactggg cgtagcgctt
     4801 cttagtgttc agaatgaata caatctttgg cagagtgcaa tatatggagc ttcagcagga
     4861 attggctttt tacttgctac agtgattttt gcgggaataa gagaaagatt ggatcttaat
     4921 gatgtgccta aatactttaa aggccttccg attgcgctta taactgcagc ttttctttct
     4981 atggcttttt taggttttaa aggaattttc gggtgaggtg agaaatgctt aaagaaatac
     5041 taatcccaat tgcaatattg gggggaatag gcttgttttt tggaattgcc ttaacgatag
     5101 caagcaaagt atttgaagta aagaaaaatg aaaaagcaga aaagatacgt cagatattgc
     5161 ctggtgccaa ctgtggtgcc tgtggctact caggatgtga tctctatgct caggctgttg
     5221 cagaaggaaa agctgaagca aatctttgta tacctggaac taatactaca gccatggcaa
     5281 ttggagaggt tcttggacta aaagttgaga aacagaagac aagagttatg aaagcattat
     5341 gctgcccaga tgttacaaag aagtttgaat ataaagggat tcagacatgt gcagctgcag
     5401 cgttattata caaaggcgaa aattcgtgca actattcctg tttggggttt ggagattgtg
     5461 ttgctgtatg tcctcatgat gcaataatga tgaaagatgg catcccaatt attgatccga
     5521 tgctttgtac ggaatgtggt ctttgtgcaa aagcatgccc taaaaacctt atacaatacc
     5581 ttattggtga atgcggatac tatgttgcat gtgtgagcca ggataccgga aaagtaacta
     5641 ataagaactg taagaatgga tgtattgcat gtggtttgtg cgtaaaagct tgtcaggatg
     5701 atgcaattca tgttataaac aatgttgcaa taatagatta tgagaaatgt acagcatgtg
     5761 gtaaatgtta tgaagtttgt ccgaaaaaga ttattaaaaa gttctgttca agctattaat
     5821 aatttatggt agaatattaa agcagattta tatatgaagg agtaaaatat ggctgaagat
     5881 aaaaaagttg taaatattga agataaagaa aaagcattaa agaaaatgag gatggaaaga
     5941 ataataacca tctctgttct tgcaatactt gtacttggaa taatatctgc tctaattttt
     6001 aaatcaatag ttggaacatg gtgggttgaa gtgcaggaag actcaacaac tacaggagca
     6061 tatatatatt ttggaggcga caagacttac aagacgttta tgtacggagt ggaccaatgg
     6121 tctgaaacag ggacatatga aataaaagga acgagtgtta acgggtacat tttatttaat
     6181 cctgaaaatg gacagcagta tataagaaga tataatatac gaggaaccag gcttaaaata
     6241 ttcaatcctg atgatacata tattagcttc ttgcgaaacc agagcaatta acaaaccggc
     6301 cctttaaaaa gggctttttt cttacataag aacccccttt gtcaatagac tttaaagggt
     6361 ttttatggta taataaatca gttaaaacta ttgaaagtga accagataaa acactatgga
     6421 aaatgaaaaa ataaacgatg tttccaaaat tataccagta gatatagaag agcagatgag
     6481 gaaagcgttc atagaatatg ctatgagcgt tattgccgac agagcgttac cggatgtaag
     6541 agatggattc aaaccagtac acagaagaat tctttatgct atgcattcat tagggtttta
     6601 ccctgaaaaa tcgcatagaa aatgtgcagc tacagtaggt gaagtattag gtaagtttca
     6661 tccacatggt gatgctgcag tatatgatgc aatggtcaga atggcacagg atttctcact
     6721 tagatatccg ctagttgatg gtcatggcaa ctttggctca agagacggag atccccccgc
     6781 agcaatgagg tatacagaat caaggcttac aaaaatagct gtggagatgc ttgctgatat
     6841 aaataaagaa actgtagact ttaaatcaaa ttttgatgac cacgaaatgg aaccggttgt
     6901 tttaccttcc agatttccaa accttatggt taatggttcc agtggaatag ctgttggaat
     6961 ggcaacaaat ataccaactc ataatcttgt tgaggttata gatggcattg ctgcggttat
     7021 aaagaatcct gatataacta ttgatgaact aatagaaatt attaaaggac cggatttccc
     7081 tacttatgga acaattatgg gaaggtcagg aatttatcag gcttacaaga caggaaaagg
     7141 ccgggtagtc gtaagaggaa aaactgagat agaagaagca gcaaatggaa agcagagaat
     7201 aataatccat gatcttcctt atcaggtaaa taaagcaaag cttatcgaaa ctgttgctag
     7261 ccttgttaaa aataaggtaa tagaaggtat ctctgaaata aatgatgaat cagatagaaa
     7321 tgaagagata aggattatta ttacactgaa aaaagatgct aatgcaaatg taattcttaa
     7381 cagattgtac aagcatacac agcttcaaga cgcattcaat gtaaatatgc tggcattaac
     7441 tccttcaaaa atgggatatg aaccaaaaat acttaatctc aaacagatgc tcgaatacta
     7501 catactgcat caggtggatg taattaagag aaggactaaa tttgatttag ataaagccca
     7561 gaccagagct catgttttag aaggtttaat aaaagcagta gacatacttg atgcagttat
     7621 tgagactata agaggcagct ccaacgaacc tgaagcaaag gaaaaccttg tatcgaaatt
     7681 tgatttttca gacagacaag ctcaagcaat tgtagatatg agattaggaa ggcttacagg
     7741 actggaaatt cttaagcttc aaagcgagta tgatgatctt ttggaaaaaa ttaaatactt
     7801 aaccaatgtt cttataaatg atgaggttac acatcaggta ataattgagg aacttgaagt
     7861 aataaagaag aaatatggtg atgaacgaag aacacgtata gaagaatctg aagaagatct
     7921 tttggatgaa gatctgataa aggaagaaac tgtagttctt actcttacaa gattcggata
     7981 tataaaaaga ttgccaacag atacttataa aagccagaac aggggtggaa aaggtgttat
     8041 tggcgttaca acaagagaag aagatacaat tatcgacata tttacaacta gcactcatag
     8101 ttatttgctt ttctttacta acagaggcag aatatacaag cttaaggggt atcaaattcc
     8161 agaagcaagc agaactgcaa aaggtactgc tattgtaaac ttgcttgaac tggagccaaa
     8221 tgaaaaagta actacagtgc ttcctataga gagttttgaa acaacacaga atcttttaat
     8281 ggctacgaag cttggttttg caaaaaagac accgctttca agttacacta atattagaaa
     8341 aggtggaatt atagcagtaa ccttgagaga tgatgacgat ttgatttcag ttaagctttc
     8401 agaaggaaat gacacagtaa tgctgattac ttacaagggt aaagctatta agttcacgga
     8461 aaaggatacc cgtgagacgg gtagatctgc aaaaggagtt attggcataa gattagatga
     8521 tgatgactat gtagtaggaa tggatttagt taaggacgag gaaactgttc taatgataac
     8581 agaaaacggt tttggtaaaa gaacattcat ttcagaatac ccgatacaga aacgaggcgg
     8641 caaaggagtt ataacatata aggttactga aaggacaggt ccggttatat cttctatgat
     8701 agcatcagat gaagatgata ttcttctaat gtctcttcag ggcgatatga ttaggttaga
     8761 tgcatcacag attagtatac tttccagagc aactcaggga gttactctga tgaggataga
     8821 tgaagagaat atggtagttg cagctgcaaa ggttgaaaaa gaagaagaat tagaagagga
     8881 tcaagaggat tcaaagaagg aataaacaat ccacaatgat tgtggaaaag taatgtgtat
     8941 aacaaattat attatgaaaa tgcccttaaa caaggcattt ttttaataca aagttatcca
     9001 caggctaacc atagattttg gggataagta ttgaaaaaat taaatttagt ccttatataa
     9061 ggaataaata ttgaatatag ttatccacag attttacaca gcaactaaca ataataatca
     9121 aagataaatg gatatatctg ttaataatct gtaaacacaa gtatgaacag aaagaaaaaa
     9181 tatttaaaaa tataccttga caggcgaggt atcttgtaat attatgtatt ctgcgaagcg
     9241 aaatactaag cgaaaggagg gattagattg ataatagcat ctgatcttat aagaggaaat
     9301 acggatacaa tcatactggc cagtttgatg aaaaaagata actatggcta tgagataaat
     9361 aaaaatatct caataaagac agacggcaaa tatgaattta aggaagcaac actttacaca
     9421 gctttcaaaa ggcttgaaga agccggtttc atacgatcct attggggaca agagaatgta
     9481 tcagcaagaa gaagatatta ctccattacc cctaaaggaa aagatgaata tcataaactt
     9541 ctagaggact ggaagaatgc aaaaaacatg atagaaaaac tgattgatac gggggtggta
     9601 gataatggat aataagttaa aggcgtatgt agataatcta tttaagaatg cgccagcaac
     9661 ggcgaaagcc tatgaggtaa aagaagaaat tcttcagaat ctcaaggata aatataatga
     9721 ccttatagca gaaggaaaga gtgagaacga agcatataag attgctattg agagtatagg
     9781 cgacttaaca gagcttttta tagaattaga aaaagagact gttcctgata actcaaagca
     9841 agagaaatat atggcactta aagctgtaac agctatgatt ttcgtacttg ctttaattcc
     9901 acttgttatg tccttttcag gaagagcagt actgttcaat cttttagcag ttgttatact
     9961 gattgctata ggtattatat tgtctgtgta taacagctca aacaagccag gctacaaata
    10021 ttatgggggg caggcttaca aaatggcaga aatagcaaaa aagaacaaca taagaaaatc
    10081 agtatccgga ttcttatggt taataactat agtgttgtat tttattgtaa gttttcagac
    10141 acatgcatgg catctgacat ggcttatgtt tctattagca gcagcaatag aatgtatact
    10201 aaatatttgg ctaggccaag ggaaagtgtg aggtgatatt atgagttcaa catcatcaaa
    10261 aataattaga acaatagcaa tactggtaat aatatttgta attataggtt taggaatctg
    10321 gtatgtagca gaatatggat ttaaaggaat cagcttttca actacgggca ggcatactat
    10381 acagactttg acaggacctt ttgaagaaaa ggcaagatat gaagctgatt ctgacgatat
    10441 agaggaaata caggttaact ggataacctc agcagtagat ttcaaggttc atgataaaga
    10501 cacaatactg tttgtagaaa gctgtcagcg agatttatca gaaggagaga atcttactta
    10561 taccataaaa gacggtatat tgaaaattac ggaacttgaa gatgaaacca tagtacgcat
    10621 agcaccaaaa aaggttgtta tctggattcc agaaagcctt cttgagactt tatcagtact
    10681 aagaagcaat tgcgctctgg ctgatgtaac tgcagaagat atctcttctg acacgatata
    10741 tcttgaatct gatacaggaa ggataaatgt aaagaatctt actgctaatc agactcttac
    10801 tctgaatacg gatactgctt caattacagc atcagatata aaagcaaaga acatagtaat
    10861 tgacagtgat acaggaaata ttgatgtaag taatttgcaa gctgatgtga aaattgatat
    10921 agagactgat actggaagag tagaagtaaa tgatgttacg acagaaacgc tgatttgcga
    10981 ttcagatact ggaagagtta tatgtttaaa ttatgatgta gattcaataa cacagagttc
    11041 cgatacagga gctttggaaa cagaaggtat ttttaaaaca ggagatttta ggagtaatac
    11101 aggaagtata acaatgaaat gtgctactct gcctgatgaa atatatgcaa agagcgatac
    11161 tggatccatc tacctttata cagttaaaac tgataacctt attgtagctt acagcacagc
    11221 tacgggaaaa tttcattcag actttcctgt acttgtatct tcctccggtg atgcaaacat
    11281 aactatgaag acagcaacag gaagtatata tatcagacca tatgagaatg aatagtaaga
    11341 aaagtaaaaa ctattaaaaa ggggctgtcg cattatgtaa ttagcgtaca gcccttcttt
    11401 taaattatta taaaaaagtt aaactactat actttctttt tgaaaagttt gaacagcata
    11461 ggatatagac agcctgcgaa tactattatt agcagatatc ttataccgtc tgcgatggga
    11521 ttttgattga ataaatctaa aagcggttgt ttcaagaatg tctttattac tattactatt
    11581 ccaagaccta cgacaagttt tataacctgt tgccaccatt ttgcttttac ttcaaaatga
    11641 gtgtacttta tgtcgacaat gacagagatt ataataccaa tacaacaacc caacagcttc
    11701 catccgtttt caattccatg cagatattca actgaccctg ttttatcaaa agaaagaaca
    11761 gctattgcta ttgtaatagc tactgccatt attccataaa ggtatgcatc tccatattta
    11821 tacttgtcag ctatacggaa aacaaagtaa cagagtacta aaagcgcaat acctattgct
    11881 aaagatgtaa atacatctgc aggtgtatgc actccaaggt acattctgga aaaagcaaca
    11941 agcagagcca atatggccat cacgatatag acccatgatt ttttcataaa catagcaata
    12001 gaaccgaaca agctcattcc ctgctgtgta tgccctgatg gaaaagagta gcctgtagcg
    12061 gcctctacag agccttcaac aggttttgct gcattttcat atgtccatgg acgaggaact
    12121 ttaaatatta gttttaaaag ctgatttaca aaagacccta aagtaaagat aaagaacata
    12181 cgataaccga tttttttatc aatacaccag atgactgcta gtatgattac agtaaagaaa
    12241 agttcttctc ccatttttgt aacaagaaga aaaaatttat cgaaaaaagg tgtcctgatt
    12301 tgttccaagg cttttagaaa ttccataaat acctcctgga tgacttcata tattactatt
    12361 ctaacttata ttataattat tttccacatt aaggtatatt tggatgtgct atttttaatt
    12421 agatttgtta tgtattttta atgaactgct ctttgcattt aggacatgta attcggattt
    12481 ttcctttgtt tttaggtaca cgtatttcgg tcttgcattt cggacattta tagtatctgt
    12541 gagtcttgtc agaaagcttt ttctttatag aatagtattt tctttttact ggattccaca
    12601 gtttcaaaaa ttgctgattt tcctttgatc ttgctgcaaa attctttgaa aaagcacgaa
    12661 atacctgata aatggccaag gctaaagaaa tatacataaa aatatcgttt cttgtgatta
    12721 aagaaagaaa aagaaatatg atgtaaaaga ttattaaagc acgattaaaa gtatccatgc
    12781 cgtatctatt cattaaaaac tctcttattt ttctcatatt ttaaattaaa actcctacat
    12841 cagtattaat gctattttac cagttgatta tgaactatat ttaaacaaat tcaatatcat
    12901 attaaaggaa taaattatta actagataac tgagcctcca tttatagaga agacttgtcc
    12961 cgtaataaat gatgtttttt cattagccag ataaagcatc atatcagtta tatctttgca
    13021 attccctatt ttcccaagag gtatcatttc ttcaagttca tcttttgtaa acttcttatt
    13081 catatcggta ttaataatgc ctggagctat acaattaact agaatattac taggtgcaag
    13141 ttctttcgcc aaagccttag taaaccctat aactgcagct ttagaagctg aatatgcaac
    13201 ttcgcatgaa gctccggtta ttccccacat tgatgatatg tttatgatat gacctttttt
    13261 gttattaacc atagaaggta ttgcacactt acaaacattg aaaagaccct ttaagtttat
    13321 atccataagc ctgtcccagt ctttttcatc catttgtgaa agaaggccca gataagttac
    13381 tgctgcatta tttataacta catcaatgtt accaaattga ttactagcat ccacaaacat
    13441 tttttgtacc tgagagtaat ctgcaatgtc agcttttata aatatgacat gatctttata
    13501 aggcagataa tctgcttcat tgatatatgt ttgattgtaa tttaccacaa cattgtatcc
    13561 tgcttctaaa aaatcaaaag ccgcttgttg tccgatacct cttgatgcac ctgttataag
    13621 gacagttctc attttccctc ctagtgatat gttagcataa aaagtgctaa tttaaaacaa
    13681 aaacaatagc actggaaact tagtaattat ttaaaatgat gtgtaagtat atgacatttt
    13741 taatatcttg tatgaatata ctaatcaata agtaaagata tatttacaaa gcttttatat
    13801 tactatattc ttaattagta caaatcggag gtttataatg aagagacata ttgtttccat
    13861 tacagttcta atagtagtcc ttgctttttg tgttgcagta ttaaacgggc aggttaatac
    13921 gagtcatgat gtagtgacgc atgagatgag atatcagaca aatgaagccg aagttgaaat
    13981 atctgaccaa cagacggtgg taaaaggagc ctgggataat tccttgagaa tttacaatga
    14041 cagaacttac tcattaattt ttaataattc ttttacagaa aagtggtatg tattggatgt
    14101 ttcagatata ggaaagtcat tatcattttt gataagttgt gatgatgagt ctgttacagg
    14161 aataattgca tcactttatt taaaagagga tattgatgaa tacggcaata atgctacaag
    14221 acttatgaat ctgtattctc taaataaatc agctacatat aaaattaaag aaacaggaac
    14281 atattatatt aagatatcca taataggaaa tagtgcagtc ggtaaggaat tgaattttgg
    14341 gtatacacta gttataaatg attcatatga agacaatgac acctggcaaa aagcagttaa
    14401 cataacgcaa ggtgaggaat atgatattga tatctctgct gctaacgata ccgactggtt
    14461 taaaataact cttccaaaga atgatcagtc atatcagtat attttaaaaa atgaaatagc
    14521 aacaagcagt aaagctcagg tattcgtata taaagaggaa gatttaaaga aatacgatga
    14581 tagcgctcag aatacattct attctgatga atttaacaag tattttacat ataaatcaga
    14641 agaatctaca acatattact taaaaatagt acctcctgtg caaggttcaa taatgaataa
    14701 aataactttt atagctgata ttatagagcc agatgctatg gagaataatg atgttttcga
    14761 taaagcggtt atgcttgaac ctgatatgtc caaaactctg actatatctg catctaatga
    14821 tatagactgg tttatgataa aaacagataa acccggacaa acagtcaggt tatcattaat
    14881 taatccttct actgctgcac aaaaactaaa ctgctggtgg tattcaaatg aaaagattat
    14941 gacatacaga gaaaccgcat caccagaata taaatcctct ggttcaggtt atacatatat
    15001 atatcttgaa gaagcttcgg tgtactattt aaaaatattt acagaagaca aaagtgttat
    15061 caaaacacct atagacttga gctatagtat tattggtgct gacaaaaatg aaccaaataa
    15121 ctcttacagt gaagcacata atatcacaag tctgcgtgaa acaagtttca cattgcctgc
    15181 attaaacgat gaagactggt ttgcgattga catagctaaa agcaatcagg ctataaggat
    15241 tgatacaagc atatcaaaaa cgggcgcgtc tgttgatgtc tccttttaca gcttaaacga
    15301 tatgcagtcg tatggagaag ctataccatt gtttagtttt aacagtaaac atgcaccata
    15361 tatatatttg cttaatgatc caggaaaata ttacatgaaa ataacatcag ataccataat
    15421 atcagacgaa tgtactttgt actattctct tatagactca gacagaaatg agccaaataa
    15481 cagtgcagaa aaagcaacta tattagaaca gggtgtaaaa acaggtttta cgctatcagg
    15541 acaaaatgat gaggactggt ttgaaataga aatacaaaat gacggtgatg ttttggaata
    15601 cataattgaa gctaagtata acagatatgc agaggttcag atttacagta aagatgaatt
    15661 gaacaaatat gatgaatatg caaatccttt attcacttca agtatttatg atgaaagtat
    15721 tacttgtaaa tttgaaaagg ccggagctta ctatattaaa ttaagttcag ataagccgat
    15781 acttgatata acttacatta catactcatt atcaaattct gatgcatatg aaaataatga
    15841 tacatggcag gatgcggttt taattgaaag aaataaggat ataaaattta ctatttcagc
    15901 ttcgaatgat gttgattggt tcaagatgga aaataaaggt atgaataaca tgagtttctc
    15961 ttataaaata cctaatacat tcatggctaa gaatataata ggcgtatttg tatatagaga
    16021 gattgatcta ttaaactatg gcttaagagc agaacctata tattcagtaa agacagcaga
    16081 cctttcaaat atgtttgaaa ttgaagaagg aacatattac tttaagatag ttagtttatc
    16141 tgatgtagca tttgaatatg aatttacttt taaggcaaat atgttctata tgcagcaata
    16201 gccatccata aaatatgtta taatatttgc ataaagtacc tcaaggagag aatgatgcat
    16261 attgaaacca aacacattaa tattaaagaa gggttctggt ataagcgcca gaatataaat
    16321 aaacaggtta ccattaaagc cgttcagaac aggtttttag aaaccggaag atttgatgct
    16381 cttgactttg ataatgaaaa aataccgcat atattttatg attctgacgt agctaagtgg
    16441 atagaaggcg tttcttttac cttatataaa gagcgaaatg ctgaactgga aacgttcata
    16501 gaccatttga ttgatcttat agagaaaaac agatctgaag atggatattt caattcgcat
    16561 tttcttagaa aacctgaaga aagatggaaa aacagagaag atcatgagtt gtattgtgca
    16621 ggccatctta tggaagcagc agttgagtat tataaagcga cgggcaaaga caaatttctg
    16681 aaactaatgc aggattatgc tgactatata tataaggtat ttgtaatcga ggattcagct
    16741 gcatttaaaa ctccagggca tgaagagata gaacttgctc ttgttaagct atatgacgtt
    16801 actgaaaata agaaatatct tgaactatcg aaatacttta ttgatacaag aggaactata
    16861 gaggaaccat atatacttga aaagtataat cactacaata ttcaggatca ccttcctgta
    16921 aggcagcaga aaactgcaga aggacattct gtaagagcga gttatcttta ctcaggaatg
    16981 gctgatattg cagtaaaata caatgataca gaactgtttg aagcatgtaa gacaatattt
    17041 tcaaatattg ttaataaaag aatgtacata accggaggaa ccggatcatc acatttggga
    17101 gaggccttca cgcttgatta cgatcttcca aattcaaaag gatatgctga gacatgtgct
    17161 gccatcgctt tagctttctt tgctcagaga atgcttttac ttgaaaagaa ttccgtatat
    17221 gcagatactg ttgaaagagt tatgtataat gggatgcttt ctgggatatc tcttgatggt
    17281 aaggctttct tttatgagaa tcctttggaa atacaacctg ttttgaataa aaggaatgta
    17341 tcaacaactt ttactgaaag aaatccaata acacagagaa aggaagtatt cagttgttca
    17401 tgctgtcctc caaatgtaat aagatttata ggagcaataa acggatacat ctattcatat
    17461 gaacaggata tattgttcat aaatcaatac atatcatcag aaatgttgta tgaaaatatt
    17521 caagctgata tgaaaacgga ttttccaagt agtggtaata ttagtataaa gactaaaaat
    17581 attaaccaaa ttgctatacg taaaccatac tggtgtactg atgtagatat caaagttaac
    17641 ggtaatgatg ctatatatac tttgaaaaaa ggatatatgt atcttggtct gactgaaacg
    17701 aataatacca tagatataaa aatggacatg ccggtaagaa ttgtgagaag taacccgaag
    17761 gtacatgaag actcaaagaa aacagcagtt atgagaggac cgatagttta ttgcgcagaa
    17821 ggtattgata atgattttcc acttaaccag attctgttta atgaaaaatg cgaatacaaa
    17881 gtaaataaat caaatgatct ggatgctgac atactgaaac ttagagttaa gataccaaaa
    17941 gaaatcgatg agttgtatac atataagcca tttgagtata tcgacaaaga attaactctt
    18001 atacctttct attgttttgc aaacagaggt gaaacggaaa tgagggtctg gttataaaaa
    18061 acaagtagca aaataaaaat gcaactgaaa aatagcactc ttgcgagggt gctattttgc
    18121 ttgtgcgaaa cgttgtatga ggagttatta ttatgtttcg gtattattag cgcctgtaaa
    18181 tttctattca gaggaggaaa ggtttttaca aaaaaggcaa atgctattaa tacaatataa
    18241 gtatattggg caagtgttaa taatttatga acaagatgtt aattttccgt gaatatttat
    18301 tctgacctta atgcttcaac agggtcgcgt tttgaagcca ttctggctgg aattgttcct
    18361 cctataacgg ttaatgcaac acttattact ataaggacaa gcgcatgtaa aggattcata
    18421 actgcaatat tatccaaatt agacaaattc tttagaatca catttgttgg taatattagc
    18481 aatcttgcaa taaatattcc aatgaatcca gaaaaagcac ctataataaa tgtttctgca
    18541 ttgaatacac gtgtaatatc tttttttctt gcacctaatg ctcttagtac accaatttct
    18601 tttgtgcgtt ccaaaacaga gatatatgtt attattccaa tcataataac agaaacgatt
    18661 agtgaaattg aggaaaaggc aataagaaca taagttatcg catccataat atttcctgaa
    18721 agcattgtaa tcatgcttgc taaatctgta tatattattt catcttcagg attttcctgg
    18781 ccggcgttat atgcatccag ataagccagt atttcgtcct ttgaatcgaa gtcagcagga
    18841 tatatgctaa tagcatatgg aactgcagtt gcacccaaaa cctgtaataa tctgtcttta
    18901 tccatttgag ctaatggaga agcgggacct aaaattgaaa atacattata gtcaacttcc
    18961 tgctgttttt gaacaacatc agaattcttt gcgttttcca ttacataact tactaaatct
    19021 tcagtatatg caacaccagc atttaatata ggcattgtgg ctttatcttt ttcacgaatt
    19081 acaccacaga tacatagagt tactgactgc tcgctgtcat atacagcctg aaaatcagag
    19141 tttacaataa aatgatcatt gaaaggtaca taaaggtcat cattcattac aagatgaaac
    19201 tgaataccaa ctatatcatc aaagctgata gtttcctgat ttggatccaa gcccagtgcc
    19261 tcaagaatat caacattgac tctgtttttt tcatctacta ccaaaaccaa ttcatacatt
    19321 gactcaggat atttgccatg taataagtcg taattggttt ccactacact caaactatta
    19381 tcatccagac ttttaggaat aactgagaaa tgtatatctg atgaactaag tattcctaca
    19441 tctccattct tcttaatcat taaattgaaa ttaatcgccc tggaatattg aagaccagaa
    19501 actaatgaag attctatctt ctctacatac tcaatatact cttcagttat attgttagta
    19561 tgtctcattg aggactgaga tggatcatat ggataaacgg cttttatgtc aggataattc
    19621 tctgatgaac cgaatatatt ttgatttctc atggtctgta attgatcata atctacattc
    19681 atagcgcttt gtgatatcat tattgggaat gctgacatgg tttcttcttc aaaagtctgt
    19741 acctgcttat cgaagccgtt tgataacgcc aaaatcaatg cgatacctat aatccctata
    19801 cttgaagcaa aagctgtaag aatagtacgt ccttttttag tcattatatt attaaaagaa
    19861 agacttaatg cagtgaaaaa actcattgca gtcttcttaa aagaatattt ttccttataa
    19921 gttggctctt cagttactgg attcgtatca tctataactt ctccatctct taaattaata
    19981 attctattgg catatttggt agcaatttct ttgttatgtg taaccataat aacaagtttg
    20041 tcttttgcaa tattctgtat aaggtctagt atctgaacgc ttgtttcagt atctaaagct
    20101 cctgtaggtt catcagctaa tattatatct ggatcattaa ctaaagctct tgctattgct
    20161 acacgctgca tctgaccgcc tgaaagctgt gaaggtttct tttttgcatg ttcttttaaa
    20221 cctacaagtt ccaatgcatc catcgccttt tttcttctct cggtttttga cacgccgctc
    20281 aaagtaaggc ttaattcaat gttcttcact acgcttatgt gacctataag gttataagac
    20341 tgaaatacga aacctacaga gttattcctg taagcatccc attcgctgtc tttgaagttt
    20401 tttgtggatt ttccatttat gacaaggtct cctgtgtcgt atctgtcaag tccgccaatt
    20461 atattaagca aagttgtctt acctgaaccg gaaggtccta atatggctac gaactcattt
    20521 tttctgaaag aaacagaaac tccattcaat gcattttgct tgaaatcgcc tactatatag
    20581 ctctttttta tatccttaag tcttaacacg tgtaagtacc ttctttctga tagctgtcct
    20641 tctaattcta tgctcagatt atgaattata tgttaacaat caaaaaatgt aaatattcac
//
LOCUS       DAOSXM010000033        20348 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2111
            NODE_1649_length_20348_cov_11.854531, whole genome shotgun
            sequence.
ACCESSION   DAOSXM010000033 DAOSXM010000000
VERSION     DAOSXM010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864178
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 20348)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20348)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:24:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,261
            CDSs (total)                      :: 2,229
            Genes (coding)                    :: 2,218
            CDSs (with protein)               :: 2,218
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 3 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20348
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1649_length_20348_cov_11.854531"
                     /isolate="AMR_MDS_2111"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            84..1268
                     /locus_tag="PLQ68_05850"
     CDS             84..1268
                     /locus_tag="PLQ68_05850"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HXK72208.1"
                     /translation="MKNLTWFKTIYAILRIPFTIYYKVFHNYHYKKYKPKNKPFLVLS
                     NHNSDGDQFMVGMAIKGHMFFVASEHIVRHGFGGRLVKLLANPIIRKKGAEAKSTVND
                     IIEALQNKSNVCMFVEGNRSFNGQTGWISPTNGQLVKKSHATLITFRLDGGYFRTPRW
                     AVNRRKGPEFGRVVNEYPYELLKDMTEDEITQIIRNDLYVNAYDWKKENQYIYKGKNL
                     AENLETVLFVCPKCKGIDTLKSNKDEFFCSCGFKVILNEYCSFENSDNDFFKNVYQWD
                     MWQRNYLKENLDTLKSEYKDKPIFSHRDQTLSLINIEKNAKKVNSGKMELFSDRLVIS
                     SENKTDIFYLSSIAGFSISKMMTIFFSTNNGEYYEIKSDSIRTGVGYLILYRYLTGRD
                     YV"
     gene            1269..2024
                     /locus_tag="PLQ68_05855"
     CDS             1269..2024
                     /locus_tag="PLQ68_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015360329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine biosynthesis TtcA family
                     protein"
                     /protein_id="HXK72209.1"
                     /translation="MKNSTVDDIIPIMSILLVSQMRKAIKDYDMIQHNDCIAVGLSGG
                     KDSLALLYCLTKIRGYIEQPFDIKAVTIKLSDTNPDTEYLKKFCENINVEYIVKQTDI
                     EKIVFESREERSPCSLCANLRRGILNDTAKSLGCNKVALGHNKDDAIETFLMCISYEG
                     RLHTFSPVTYLSRKDITVIRPLIYSWEDEIIQFNDKMGITPVKTLCKAAGVTNRQRIK
                     ELIEEEALINPNFKYNLFRSFTKGEISRWHEVK"
     gene            2024..3445
                     /locus_tag="PLQ68_05860"
     CDS             2024..3445
                     /locus_tag="PLQ68_05860"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010244952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HXK72210.1"
                     /translation="MVFSGLFFLTIFLPVSLILYYAVKNRTYRNVILTIMSLVFYAWG
                     EPVWILLLLFCSMFVYLFAIMIERSNRDNLSKTLLIVSIAILIAILGFFKYSGFIVDN
                     ISSLFSIENSYTDMALPLGLSFYTFTCISYLVDVYKKKAPAQRNYVNMLMYISLFFTV
                     TSGPIQRYPDLLADINANRESWSKFNYGVHRFIIGLAKKAIIANTAGSLISSFMGNDY
                     TKLSVQGAWIGILLFSLQIYFDFSGYSDMAIGLASMFGFSLKENFNYPYISRSATEFW
                     RRWHISLGSFFRDYVYIPLGGNRKFKLRNLFIVWFLTGLWHGASWNFVIWGLYFFVFL
                     LIEKTFLQKIFDKIPKIFSHIYFVLVMLIGWVFFYNTDLIQAIRYIGVMFNINAVSFS
                     DPILTILFNNNVLFIIGAVICAVPLGKLLKQVFEKLENKISNGRTDIVNSVIIPLINI
                     ALLAVSIIFLVGDTYTPFLYFNF"
     gene            3446..4822
                     /locus_tag="PLQ68_05865"
     CDS             3446..4822
                     /locus_tag="PLQ68_05865"
                     /inference="COORDINATES: protein motif:HMM:NF025644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHHW family protein"
                     /protein_id="HXK72211.1"
                     /translation="MKKNRLDATIFIIIIFLAGLINLFNPNKPTISVAENRKLQEKPV
                     FSIESVFNGSYAKEYSSYYSDTFILRELLMQSKRQISKSFALIKDMKIVIEQPQASTS
                     PSFQPSNTPHQSQLPELSLDISPVPSSSGTPLPSPTPSEEPITEPEYRNVNTVEVLNT
                     PFLLVDNHVVATLWASDESLRKYAQFLNELKISMGEDVSIYSMTVPTSIEYFDLSSLP
                     NITGSQKKIVDDINSYTDSSIKDVDVYGALFNHCSEYIYYKTDHHWTHLGAYYAYTAL
                     MESMNKAESIIPLGDYTEKEIIEGYIGSAYRKTNQDERVLNNLDILTAYYPVIDYKFI
                     MHKKDEQIEKRLNDLSYISSDNQYYNMFMSGGNGIYHEFTTTNKNGQTLLLICDSYGA
                     SFVHFLLPHYEKVYIMDTRYYDKEYLNGMTIDEFAKSINATDISTLMYMDSVAYLFNT
                     EVLYSLLK"
     gene            complement(4933..5805)
                     /gene="galU"
                     /locus_tag="PLQ68_05870"
     CDS             complement(4933..5805)
                     /gene="galU"
                     /locus_tag="PLQ68_05870"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002582387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HXK72212.1"
                     /translation="MKKIRKAIIPAAGLGTRFLPATKATPKEMLPVVDTPTIQYIVQE
                     AALSGIEDIVIISGRGKRAIEDHFDKSYELEDILLKHNKTEELEQIRNISQMANIYYI
                     RQKEAKGLGHAILCAKTFIGHEPFAVLLGDDLVYSDKPCLSQLIEAYEKYDASIVGVQ
                     KVPKDKVNLYGIIDGNPIQPKVMKVKDMVEKPDYEHAPSNTAILGRYILSPEIFDVLE
                     KQKPGKNGEIQLTDALRSLLEIQDIYSYEFEGIRYDLGDKLGFLEANIEYALRRMDLQ
                     EDLKIYLKDIANRL"
     gene            complement(5816..8041)
                     /locus_tag="PLQ68_05875"
     CDS             complement(5816..8041)
                     /locus_tag="PLQ68_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015924899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HXK72213.1"
                     /translation="MKKDNKDNKDNIKNQNHTKPQPITEILETNFMPYAMSVIVSRAI
                     PEIDGLKPSHRKLLYTMYKMGLLRGDRTKSANVVGQTMKLNPHGDQAIYETMVRMTRG
                     NEALLHPFVDSKGNFGKQYSRDMSYAASRYTEVKLASISGEMFRDIDKDCVPFTPNYD
                     GTIMEPVMLPSAFPNILINASQGIAVGITSNLPSFNLNEVCECVIAYIENRDINIADY
                     IKAPDFSTGGYVIYDKDEMDNIINNGRGKVRLRAKYNVDKKGTHIEITEIPYTTSAEA
                     IIENIIGMVKSSRIKEVQDVRDETDISGLKITIDIKKGTDSEKLMAKLFAKTPLEDSF
                     SCNNNVLIEGKPLLLGVRDIIDYWIIFRVECIKRQLRYDLGKDKERLHLLLGLERVLL
                     DIDEAIRIIRNTELESEVIPNLMEAFKIDELQAEFIAEIKLRHLNREYILKRLDDIND
                     LNKSIKNAEETLKSRSRIEGIIASQLKDIMTKYGQPRKSEILYEHQIEEVFEEPEFED
                     YPVKIFFTKDNYLKKIRETDFKPEAENKFKNDDVLMQELTSRNKAELVMFSSKGNVYK
                     SRLYQIEDCKITDFGEYLPNICELEKGERIIYIAATEDFKGNMIYLFENGKGAKIPFK
                     SFWTKQNRKKILNAYNMDTRLLYISLIDENDDTSYVAITSDQRALIFNSKDISLKGKR
                     DTQGVTVIKLKSKTVVTEMLPLEVARLKDTDKYMPSNVPAVGKKLSEKDKRRKQVSLF
                     D"
     gene            complement(8045..10042)
                     /locus_tag="PLQ68_05880"
     CDS             complement(8045..10042)
                     /locus_tag="PLQ68_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007493288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HXK72214.1"
                     /translation="MAVKNTYNNESIKSLKGPDRVRKCAPVIFGSDGIEGCQHSVFEI
                     VSNSIDEARAGYGKLIEVIRHADRSITVKDHGRGMPVDFNKNEQRYNWDLIYCELYAG
                     AKYDGNLAGNYDASIGLNGLGCCATQYASSYMDVEVIRDGYKYNLHFEEGFNIGGLKK
                     KEEKGKKTGTTTRWKPDPKVFTDINIPIEYFQDMLKRQAVANTGLKFILTDEESNKTY
                     EYLYENGIKQYVEEVLADRTATSLVYISRDTKLIERDGSREHDAKLTACFCFSSEANL
                     IEHYYNSSYLVNGGSPERAMRAAFTFALDKFMTSEGKYTKGEAKITYYDIQDSLIFVS
                     NSFSSVGGFENQTKKSLVNAIFQDAMTEFLKHELEIYFIENKSEALKIADQVLLNKRS
                     SEQAEKTKLNLKKKLGKGLDMNNRVKKFVDCRTKDVNKREIYIVEGDSALGSTKMGRD
                     AEFQAIIPVRGKILNCLKAEYNRIFESDIIIDLLKVLGCGVEIKTKSNKKISQFDMSK
                     LRWSKIIICTDADVDGFHIRTEILTMIYRLIPSLIEEGKVYIAESPLFEISYKKETLF
                     AYSENEKNEIVEKIRSKEQKNGQVPKISIQRSKGLGENEPDMMWLTTMNPATRRLIKV
                     MPTDMKQMDDMFDLFLGDNLQGRKDFIQEYGYKYTDMLDVS"
     gene            complement(10178..11095)
                     /locus_tag="PLQ68_05885"
     CDS             complement(10178..11095)
                     /locus_tag="PLQ68_05885"
                     /inference="COORDINATES: protein motif:HMM:NF013078.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HXK72215.1"
                     /translation="MPDVLTHILFARKVKDTISDVFVKEAIEKNIQIYNFGAQGPDFL
                     FYHASPLLVDNRVRSAGAMMHRIETAKFFKDYVLWLENLNGEEYEQALAYFAGFLTHF
                     YCDKTIHPYVEATREQGCGYFEKKQEKAHLSHYMIEYIMDIRLWKKYTDVDAYKQNIV
                     ELIGEDPLPYEIVRYITEFINFTQPNAISKKQVYNSSVLMKRIHKILFDPKNLKKWFI
                     NILPLPRRCYVEKSKEDIDVLNLNRRKWHHVLNEEEISDSSVEDLLEIGSKECSKCLN
                     EIIEMLEKGIGKDLNTLIPDVGYMTNKPV"
     gene            complement(11095..12723)
                     /locus_tag="PLQ68_05890"
     CDS             complement(11095..12723)
                     /locus_tag="PLQ68_05890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72216.1"
                     /translation="MKSHYKDIVKGNESAIEHYLKSFNYEEKVMYGAYGYPDYANNSV
                     PIETIASGYYCVDSIYHSDSRLIYLMNCMIDYLYTAHRPDFTVDNRESDYCAPPILVS
                     IYLSRAYRIMEKYAQTKEQIALKDRLREYLEFPAKGISNGGIITPNHRWMGSSSALIL
                     YSIVKNKGLTDFAYGYLKEGVDIDEFGEYTERSPMYNRECDNAFIIIAEETKDNYYLE
                     CVRRNLELMLYYIEPDFTMFTQNSNRKDKQEGDVSASFYPDGFYHAYMYMSYLLKNPV
                     FARMADDIFRNIKSRVDRGPSILWPFLLNPELMSYEPVLGDRPITFNIFHKDIVRRKN
                     DDFSVTLLTRSPNFLFIQKGNLRCFVRICASFFAVAQFIPADIKKIDDDTYYMTMTGE
                     GRYRGPLPDKPETSKWKEMDHSKRPIINQVQLTYHVKITLHDSKVELDIRTEGMDQVP
                     YKVEFCFSAPCSVKNQNNEYEGQAGKHMTFKDADIDIQKDNNTLRITNTHNSHIYHNI
                     MRGSVQKSMNSFTIYYTGFTHIDKHIEILEVGKS"
     gene            complement(12727..14355)
                     /locus_tag="PLQ68_05895"
     CDS             complement(12727..14355)
                     /locus_tag="PLQ68_05895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72217.1"
                     /translation="MKKHYLGNVRGNEASIDWLLSRFDEKIKAIKNGYGYASYGANST
                     SLDTLASGYFCSDSKHYKNSQVLDLMNDVLTFIDSLHRPNYTIDMMESNFYCAPLFEA
                     INMARAYRIIDKYAQTDREKDFLRRYREYLPKVEKSLLNGGIHTPNHRWIGTSAMMMM
                     ANILKSDELRKMGEAYLNEGIDMDEFGEFTERSPHYNEVCDTSFMQIALETGKMEYLE
                     YAKKNMDLMFNYIEPNFTVFTQNSNRQDKHEGDINAITPPNYLYYTYAHMAYITKNGQ
                     YAKFADDIYNSIVEKHGRGPGLLHMYMLYPELLDFDPVLEDYPTSYNIFHKDIVRFKK
                     DDFSATLITRSPNFLFVQKGDLRCFVRICASFFAVAQFKPLEINKIEDNTYQMKMKAH
                     GEYYGPMSEKPSTSKWKEMDHSLRPVNNMIDLEYTVTVKIENDRAVLNVRTEECDNVP
                     MKIEFCLSAPVTAKFNNEIIAGEPDGNLCVNAEEILISKGQDALKFTNSFCNHTYHKD
                     MRGSIPPSKGAFTVYYTGFTHIDKKIDIIGTKEA"
     gene            complement(14360..15307)
                     /locus_tag="PLQ68_05900"
     CDS             complement(14360..15307)
                     /locus_tag="PLQ68_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004627201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HXK72218.1"
                     /translation="MYSIGIDLGGTAIKAGVVDELGNIVIKDKVPTKSERGYEAVIAD
                     MADLCKRLLGEANIDEKNVSRIGVGCPGTPDKTNGILIYNNNLGMRNVNIRKELQKYF
                     NLPVYIDNDANVAALAESVCGASKNTSCSVTITLGTGVGSGVVIDNRVYSGFNFAAAE
                     LGHHTLVMDGEACTCGRNGCYEAYASATALISQTKRAALLNKDSLIWKACNNNLDNIS
                     ARTPFEAERMGDKVAKDVCDTYIKYVGEGLVNIINIFQPQVIAIGGGVCNEGERLLNP
                     LKEYIEKYKYTREETPASTLVIAQMGNDAGIVGAAMLVE"
     gene            complement(15319..16116)
                     /locus_tag="PLQ68_05905"
     CDS             complement(15319..16116)
                     /locus_tag="PLQ68_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014312140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HXK72219.1"
                     /translation="MQGITIGQYIPGESVVHKADPRLKIAITILTMVLLLILDSFYAL
                     LLAAIFVVSAFLVSKVSLRYALKGLKPVIFIVIFTMILNIFFATGTPLYTFWIITITK
                     EGLVTSALISVRILLLISAGSLMTYTTTPLVLTVGIERLMSPLKYIKVPTAEIAMMIS
                     IALRFIPTLMEETEKIMKAQAARGADIGTGNIFQRAKSFIPILIPLFISSFKRADDLA
                     VAMDSRCYHGSEGRTSISELKYTSIDLSIFIVYILFVAVALVLQYAL"
     gene            complement(16174..16647)
                     /locus_tag="PLQ68_05910"
     CDS             complement(16174..16647)
                     /locus_tag="PLQ68_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004624202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HXK72220.1"
                     /translation="MKKHVKDSYTEQAQILTQCTLNGNSRLFGGQMMEWIDVTAAIAA
                     RRHSNRNVTTVAVDSLEFKGPAYANDTVLLTAKVTYVGRTSMEVCVKSYVEDLIGEKK
                     LINEAYVVMVALDEFERPAEVPGLIIETEEEKCDWEMGKKRYILRKERKKESECT"
     gene            complement(16667..17575)
                     /locus_tag="PLQ68_05915"
     CDS             complement(16667..17575)
                     /locus_tag="PLQ68_05915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72221.1"
                     /translation="MYYILSSYDYTALINLSGIEQNKISQAVFNPDINNRSSLSLAKN
                     ELAKLGLVKKGAITLSGRLIAEAILSPESVISVVSCNAIDMATAHYCYANGFWVRILP
                     NIDHTIITIETPIVNKDLGMKIKKDFLSDMDFEEGLGIDVSMTVGELIIFEVMQLALV
                     DKVRKKGAPLNKDESAMSISEFINRKNILDASSAALILGDKDGYEISKNFWDTDNIIS
                     DIDELVKKDIFNRVTEVKTGKKKYIMSEMSKRWLTLDSLVDKITIQKLPEGDIINLSI
                     TKAGIMTVRQNKQMLYFKTIAVEQLY"
     gene            complement(17584..20019)
                     /locus_tag="PLQ68_05920"
     CDS             complement(17584..20019)
                     /locus_tag="PLQ68_05920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HXK72222.1"
                     /translation="MARVCPNCGLHVSDKAKYCRKCGTPIGITNVSDYSFSKNMVQFS
                     KPWENSKSRLGLKGIKSILMDFRKGIRRATYLIPVYVLAAVWLVISLLFALEIRTPLL
                     NFFAFLTYSDGAMSGGLLGAVGGIVGKMIYLYFIVMLLTPVYSLQKPFKGISTGLRMF
                     FNSMLRFKNLKDIYIPVLTAGLSFIGFNMFTGNNNWINSIISPVIVIIILRAIGRKSG
                     ITWDIFHRVFGKSMSEDITIRSLGGYIVGILLAIPCFLFSSAFICYYIGLLLIFISLV
                     IRLIDVLIIRRAGFRRIMPIILATIILLSPISVLAAPVNEIKGIWVLHDINIIDPDTN
                     ENTKENTYVSDGYIRKHYSVDATYNIEYFWDIPPKKIYAGNETKLLINQKVVYSGSED
                     TKQTSSLKTSMFITYIPDDKNPEINEYFKASKDFATENMFLAHEQDYTAFFLKILNYY
                     DLYKELNKNNCRLTISVQASFGGRVEYVYNLQKSPTDQFFTWFAGSGSDNEHYKPWGT
                     VITNFITITAALMGATVSVGSLTLRRKKSTSKTTQKKTDIIQKGKNAGYDNNISTSDL
                     SDNLTNLYDSLSKASAAGWPKYKEVFQKTLSKSKDHIIQSKSIYAIESSINRYRNSVS
                     GLIHDLSALGYNNDAHALDIRGEEITNATGKIVYSNYVFNSLASGNPYCAFIELANAF
                     ALENMKAGVLVSPSVHLRGIMSIGLDSINDAQTAYRNALNGRYGDKIRNMSRKGEITY
                     ETVQKFTQTVIKSDELDKLYESVINGTLTEEEYKEISSYRVYSTKRAVKQAQTHNFKY
                     EETSLVVKSLFDW"
     gene            20120..>20348
                     /locus_tag="PLQ68_05925"
     CDS             20120..>20348
                     /locus_tag="PLQ68_05925"
                     /inference="COORDINATES: protein motif:HMM:NF014669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015225 - biotin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015878 - biotin transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HXK72223.1"
                     /translation="MASRTKQIAYTGMFTAIIAVMSQLAIPMPSGVPATLQTLAIVIT
                     GLILGVKAGTVSVGLYITIGAIGLPVFSAFRS"
BASE COUNT         6147 a         3888 c         3156 g         7157 t
ORIGIN      
        1 acagtaacat cgactttttg tatttgtttt taagtatttt ttttgataat attattatta
       61 accttatatt catggagagt actatgaaaa accttacctg gtttaagacc atttatgcaa
      121 ttcttagaat accttttacc atatattaca aagtatttca caattatcac tataaaaaat
      181 ataagccgaa aaataaacct tttttagtat tgtcaaacca caattccgat ggggatcagt
      241 ttatggtggg gatggcaatt aaagggcata tgttttttgt agcaagtgag catatagtaa
      301 ggcatggttt cggaggcagg cttgtaaaat tacttgcaaa tccgataata aggaaaaagg
      361 gagcagaagc taaaagcaca gtaaatgata ttattgaagc cctacaaaac aaatcaaatg
      421 tatgcatgtt tgtagaaggc aaccgttctt ttaacggtca gacaggatgg atatcaccta
      481 ccaatggaca gttagtaaaa aaaagtcatg ctacattaat aactttcagg cttgacggag
      541 gctatttcag aactccaaga tgggctgtta acagaagaaa gggaccggag tttggaagag
      601 tagtaaatga atatccatac gagctgctaa aggatatgac agaagacgaa ataacccaga
      661 ttataagaaa tgatctttat gttaacgcat atgactggaa aaaggaaaac cagtatattt
      721 acaaagggaa aaatctggct gagaatcttg aaactgtatt gtttgtttgc ccgaaatgca
      781 aaggtattga tacattgaaa agtaataaag atgaattttt ctgttcttgc ggttttaagg
      841 ttattctcaa tgaatactgc agttttgaaa attctgataa tgattttttc aaaaatgtat
      901 accaatggga tatgtggcag agaaattatc taaaagaaaa cctggatact ttaaaaagcg
      961 agtacaagga taaacccata ttcagtcaca gagaccagac acttagcttg attaatattg
     1021 aaaagaatgc aaaaaaagta aatagcggaa aaatggaatt attcagtgac agacttgtga
     1081 tatcttcaga aaataagaca gatatatttt acttatcatc aattgcagga ttttcgatat
     1141 caaaaatgat gactattttc ttttctacta ataatggtga gtattatgaa attaaatcgg
     1201 acagtataag aactggagta ggatacctaa tactgtacag atatcttacc ggtcgtgatt
     1261 atgtatgatt gaaaaacagt actgtagatg atattattcc aataatgagt attttactag
     1321 tcagccaaat gcgaaaagcg attaaggatt acgacatgat acagcataac gattgtattg
     1381 ctgtcggact atcaggcgga aaagacagcc ttgctttact atattgctta acaaagattc
     1441 gaggttatat agaacagcct tttgatataa aggcagtaac aataaagctg agcgatacaa
     1501 atccagacac tgagtatctt aagaaatttt gtgaaaacat aaatgtggaa tatatagtaa
     1561 aacaaactga tattgaaaaa atagtttttg aatcaagaga agaaagaagc ccctgctcgt
     1621 tatgtgcaaa cttaagaaga ggcattttaa atgatacagc aaagtcactt ggatgcaata
     1681 aagtcgcatt aggtcataat aaagatgatg cgatagagac ctttcttatg tgcatatcct
     1741 atgaaggaag acttcataca ttctcgcctg ttacatacct ttcaagaaaa gatattacag
     1801 tgatacgtcc tcttatctac tcatgggagg atgaaatcat acagtttaat gataaaatgg
     1861 gaataactcc tgtaaagacg ctgtgcaagg cagcaggagt aactaatcgc caaaggataa
     1921 aggaactgat agaagaggaa gctttaatta atcccaattt caaatacaat ctcttccggt
     1981 cctttaccaa aggtgaaata tcaagatggc atgaggtaaa gtaatggtat tttcaggatt
     2041 attcttttta acaatatttc tccctgtatc ccttattctt tactatgcag taaaaaacag
     2101 gacatataga aatgtaatac tcactattat gtcactggtt ttctatgcgt ggggagaacc
     2161 tgtatggata cttcttctgc tgttttgttc aatgtttgtt tatctatttg caataatgat
     2221 agaaagaagt aacagggata acctttctaa aacactttta atagtttcca tagctatatt
     2281 gatagcaatt ctgggattct tcaagtattc tggttttatt gtggataata tttcatcact
     2341 tttctccatt gaaaacagtt atacagatat ggcattgcca ttaggactct ctttttacac
     2401 ttttacatgc ataagttatc ttgtagatgt ttataagaaa aaggctcctg cacaaagaaa
     2461 ttatgttaat atgctcatgt acatatcctt gttttttaca gtcacatccg gacctataca
     2521 aagatatcct gatcttcttg ctgatataaa tgcaaacaga gagagctggt caaaatttaa
     2581 ttacggagta cacagattta ttataggact tgcaaaaaaa gcaataatag ccaatacagc
     2641 aggttctttg atatcgtcct ttatgggaaa tgattacact aaactatccg tacagggagc
     2701 atggattggg atactgctgt tttctttaca gatatatttt gatttttccg gatattcaga
     2761 tatggcaatc gggcttgctt ctatgttcgg tttctcttta aaggaaaatt tcaattatcc
     2821 ctacatttcc agaagtgcaa cagaattttg gagaagatgg catatatctt taggttcctt
     2881 ttttagagac tatgtataca tcccgcttgg tggaaacaga aaattcaagc ttcgcaacct
     2941 tttcatagta tggtttttaa caggcttatg gcatggtgcc agctggaact ttgtcatatg
     3001 ggggttatac ttctttgttt ttttacttat tgaaaaaaca ttcctacaga aaattttcga
     3061 caagatccct aaaatattct cgcacattta ctttgtactg gtaatgttaa taggatgggt
     3121 attcttctat aacactgacc ttatacaggc tatacgatat attggggtta tgttcaatat
     3181 aaatgcagta tcattttcag acccgattct taccatactg ttcaacaata atgtgctgtt
     3241 tataattgga gcagttatat gtgctgtgcc tttgggaaaa ttactaaaac aggtatttga
     3301 aaaattagaa aacaagattt ctaacggtcg gactgatatc gtaaacagtg ttattattcc
     3361 tcttataaat atagctcttc ttgcagtatc aataatattc cttgttggcg atacttacac
     3421 accgtttctt tactttaact tttaaatgaa aaagaacaga ctggacgcta ctattttcat
     3481 aatcataata tttttagcag gtttaataaa tctatttaat ccaaacaaac ctactatatc
     3541 agttgctgaa aaccgaaaac ttcaggaaaa acctgttttt tcgatagaat cagtattcaa
     3601 cggttcttat gcaaaagaat attcttcata ctattctgat actttcattt tgcgtgaact
     3661 gctaatgcag tcgaaaagac agataagtaa aagttttgct ttgattaagg atatgaaaat
     3721 cgttatagaa caaccccaag cttccacatc accatcattc cagccaagta atacaccaca
     3781 ccagtcacaa cttccagaac tgtctttaga catatcacct gttccttcct cttccggtac
     3841 acctttacca tcaccaacac catctgaaga accaataact gaaccagagt atagaaatgt
     3901 taatacggtt gaagtgttga acactccttt tttgcttgtt gacaatcatg tagtagcaac
     3961 actatgggca tctgatgaaa gcctgagaaa atatgctcag ttccttaatg agctgaaaat
     4021 atcaatggga gaagatgtaa gcatatattc aatgacagta cccaccagta ttgaatattt
     4081 cgacctttca tcattaccta atataaccgg gtcacagaaa aaaatagttg atgatataaa
     4141 ctcatatact gacagcagca ttaaagatgt tgatgtttat ggtgctcttt ttaatcactg
     4201 cagtgaatat atttattaca aaaccgatca ccactggaca cacttaggcg catactatgc
     4261 ttatactgct cttatggaat caatgaataa agcagaaagc atcattcctc ttggtgatta
     4321 cacagaaaag gaaattatag aaggatatat aggaagcgca tacagaaaaa ctaatcagga
     4381 tgaaagagtt ttaaacaatc tcgatattct gacagcatat tatccagtta ttgattacaa
     4441 attcattatg cacaaaaagg atgaacagat agaaaaaagg ctaaatgatt taagctatat
     4501 atcttctgat aatcagtatt acaatatgtt catgagcgga ggtaacggga tataccacga
     4561 gtttaccact accaacaaga acgggcagac cctgttgttg atttgtgatt catatggtgc
     4621 aagctttgta cattttcttt tacctcatta tgagaaagta tacataatgg acacaagata
     4681 ctatgataaa gaatatctaa atggtatgac tattgatgaa tttgcaaaaa gcataaatgc
     4741 aacagatata tctacactta tgtatatgga tagtgtggca tacctgttca atactgaagt
     4801 gttgtattca ttattgaaat agaactatgt atgatttaat aaaaatcatt tatgaattaa
     4861 aaataaattt tcaggaattt tcagaggtaa tacaaaaact ctagtaatat taagaaaaga
     4921 atatatgtta atctataatc tgtttgcaat atccttaaga tatatcttta ggtcctcctg
     4981 caaatccatt cttctaagag catattcaat attagcttct aaaaacccaa gtttgtcccc
     5041 cagatcatat cttattcctt caaactcata tgaataaatg tcctgtattt caagaagtga
     5101 ccttaatgca tcagttagct gtatctcccc attttttcct ggtttctgct tttctaaaac
     5161 atcaaatatt tctggtgata aaatatatct tccaagtatt gctgtatttg aaggagcatg
     5221 ttcataatct ggtttttcaa ccatatcttt aactttcatt actttgggtt gtataggatt
     5281 tccatctatt attccataaa gattaacttt gtcttttggt actttctgta ctccaactat
     5341 cgatgcatca tatttctcat atgcttcaat aagctggctt aaacatggct tatctgaata
     5401 aacaagatca tcacctaata atactgcaaa aggttcatgc ccgataaatg tcttagcaca
     5461 aagtatggca tgtcctaatc ctttggcttc cttctgcctg atataatata tattagccat
     5521 ttgtgaaatg ttcctgattt gttcaagttc ctcagtttta ttgtgtttta gtaaaatgtc
     5581 ttctaattca tatgacttgt caaaatggtc ttctatcgct cttttacctc ggcctgatat
     5641 tatgacaata tcctcaattc cagataaagc agcttcctga actatgtact gaatggtagg
     5701 tgtgtcaact accggaagca tttctttggg cgtagctttt gtggcaggaa gaaatctggt
     5761 tccaagtcct gctgcaggta ttatggcttt tctaatcttt ttcatttcca ctcctttaat
     5821 cgaacagtga aacctgtttt cttctcttgt ccttttctga cagtttcttt ccaaccgctg
     5881 gaacgtttga tggcatatac ttatctgtat cttttagacg ggctacttca agaggaagca
     5941 tttccgtaac tactgttttg ctcttgagct taattacagt aactccctgc gtatctcttt
     6001 ttccttttaa tgatatatcc ttactgttaa agataagcgc tctttggtct gatgttatag
     6061 caacatagga tgtatcatca ttttcatcta taagtgagat atataaaagc cttgtatcca
     6121 tattatacgc attaagaatt tttttcctgt tctgttttgt ccagaaggat ttgaatggta
     6181 tttttgcgcc tttaccattt tcgaacagat aaatcatatt acccttaaag tcttcagtag
     6241 cggctatgta tataatcctt tcaccttttt caagttcgca gatatttggt aaatattctc
     6301 caaagtctgt tatcttacag tcctctatct gatataacct ggatttatat acatttcctt
     6361 tgctggagaa cattacaagt tccgctttat ttcgagatgt cagctcctgc ataagaacat
     6421 cgtcgttctt aaatttattt tctgcttcgg gtttgaagtc agtctctctt atctttttaa
     6481 gataattatc ctttgtaaag aagattttta caggatagtc ttcaaattcc ggctcttcaa
     6541 aaacctcttc tatctgatgt tcataaagta tttccgattt tctcggctgt ccgtatttag
     6601 tcataatatc tttaagctgt gaagctatta taccttcgat tcgacttctg ctttttaaag
     6661 tctcttcagc attttttatg cttttgttta aatcatttat atcatctaac ctctttaaga
     6721 tatactccct gttaagatga cgcagtttga tttctgcaat aaattctgcc tgcagctcat
     6781 caattttaaa tgcttccatc aggtttggta tgacttcgct ttccagttca gtattacgta
     6841 taattcgtat agcttcatct atatctaaaa gaaccctttc aagtcctaac agaagatgaa
     6901 gtctttcttt atcttttcct aagtcataac gtagttgtct tttaatacat tcaactctga
     6961 aaattatcca gtaatctata atatccctta ctcctaataa aagaggtttt ccttcaataa
     7021 gtacattatt attacatgaa aatgaatctt caagtggtgt ttttgcaaat aacttagcca
     7081 tcagcttttc tgaatcagta ccttttttta tatctatggt tattttcaat ccgcttatat
     7141 ctgtctcgtc acgtacatcc tgaacttctt ttattctgct ggattttacc atacctatta
     7201 tattctcaat aattgcttct gctgatgtgg tatatggtat ctcagttatc tctatgtgag
     7261 ttcccttttt atcaacattg tatttagctc taagccgtac ttttcccctt ccgttattta
     7321 tgatgttgtc catttcatct ttgtcataaa ttacatatcc gccagtggaa aaatcaggtg
     7381 ctttaatata atcagctata tttatatctc tgttttcaat atatgctata acgcattcac
     7441 agacttcatt taaattaaac gaaggaagat tacttgttat acctactgct ataccctggc
     7501 ttgcatttat aagtatgtta ggaaatgctg aaggaagcat tacaggctcc atgattgttc
     7561 catcataatt tggggtaaag gggacacagt ctttatctat gtcacgaaac atttctccac
     7621 ttatagatgc aagtttgact tctgtatatc tgcttgcagc ataactcata tcccttgaat
     7681 actgctttcc gaaatttcct ttggaatcca caaaaggatg taaaagagcc tcatttcctc
     7741 tggtcatcct gaccatggtt tcataaatcg cctgatctcc atgaggattt aatttcatag
     7801 tctgtccgac tacattggct gactttgtgc ggtcacctct taaaagtccc atcttgtaca
     7861 tagtataaag aagcttccta tgagaaggtt ttaaaccgtc tatctccgga atagcacgcg
     7921 aaactattac actcatagca tacggcatga aattagtttc caaaatttca gttataggct
     7981 gtggtttagt atggttttga ttttttatgt tatctttatt atctttgtta tcttttttca
     8041 tatattatga tacatcaagc atatcggtat atttatatcc gtattcctga ataaaatctt
     8101 tcctcccctg taaattgtcc cctaaaaaca aatcaaacat gtcatccatc tgcttcatat
     8161 ctgtaggcat tacttttatt agccgtcttg tagcaggatt catagttgta agccacatca
     8221 tatcaggctc gttctcaccc agaccttttg acctttgtat tgaaattttt ggaacctgac
     8281 catttttctg ttcttttgaa cgtatctttt caactatctc atttttttca ttttcgctat
     8341 atgcaaacaa tgtttccttc ttgtatgaaa tctcaaacag aggcgattct gctatatata
     8401 cctttccctc ttcaatcaaa gaaggtatga gcctgtaaat cattgtaaga atctccgtcc
     8461 ttatatggaa tccatccaca tcagcatcag tgcatattat tatctttgac caacgaagtt
     8521 tgctcatatc gaactggctt atctttttat ttgatttggt ctttatctct acaccgcatc
     8581 ctaaaacttt gagaaggtca attatgatat cgctttcaaa tattctattg tattctgctt
     8641 taaggcagtt gagtattttt cctcttacag gtattattgc ctgaaactct gcatctcttc
     8701 ccatttttgt agaacctaaa gcagaatcac cctcaactat atatatctct cttttattta
     8761 catctttagt tctgcagtct acaaattttt taactctgtt gttcatatcc aaaccttttc
     8821 caagcttctt ctttaaattc agcttggttt tttccgcttg ttctgaactt cttttattca
     8881 atagaacctg atcagcaatc tttaatgctt ctgatttatt ctctataaaa tatatctcca
     8941 actcatgctt taaaaactcc gtcatggcgt cctggaaaat tgcattaaca agactctttt
     9001 ttgtctggtt ttcaaatcct ccgactgatg aaaatgaatt tgatacaaat atgaggctgt
     9061 cctgaatatc ataatatgtt atctttgctt cgccttttgt atacttgcct tctgatgtca
     9121 taaacttatc cagtgcaaat gtaaatgcgg ctctcatagc tctttcggga gaaccaccgt
     9181 taacaagata acttgagtta tagtaatgtt caatcaggtt tgcttctgat gaaaaacaaa
     9241 aacaagctgt aagtttggca tcatgttcac gagatccgtc tctttcgata agttttgtat
     9301 ctcttgagat atacaccaat gatgttgctg ttctgtctgc cagtacttct tcaacatact
     9361 gtttaattcc attttcatac aggtattcgt aagttttatt ggattcttca tcagtcaata
     9421 tgaattttag acccgtattt gctactgcct gccttttgag catatcctga aaatattcaa
     9481 ttggaatatt tatatcagta aagactttcg gatcaggttt ccacctagta gtagtacctg
     9541 tcttcttgcc tttttcttct tttttcttaa gtccgcctat gttaaatcct tcttcaaaat
     9601 gaagattata cttatatccg tcgcgaataa cttctacgtc catatatgaa gaagcatact
     9661 gcgtagcaca gcatcctaaa ccattaagac ctattgatgc atcataattt ccagcgagat
     9721 taccgtcata tttagctcct gcatacagtt cgcagtatat taaatcccag ttatatcttt
     9781 gctcattttt gttaaaatca acaggcatgc ctcgtccatg atcttttaca gtaattgatc
     9841 tgtccgcatg cctaataacc tcaataagct ttccataccc tgctctggct tcatcaatag
     9901 aattagaaac tatttcaaat accgaatgct ggcatccttc aattccatct gaaccgaata
     9961 taaccggtgc gcattttcta actctgtctg gacctttaag ggattttatg ctctcattat
    10021 tgtatgtgtt ttttacagcc attaataacc gccttttctt ttgtatatat catatagatt
    10081 atattccatt tataggttat tttcaatata cctgttggat gaatattttg cagtgtttta
    10141 agtattctta atatttataa tatcagttta attaaaatta aactggttta ttagtcatat
    10201 accctacatc aggtataagg gtgtttaaat cttttcctat tcctttttcc agcatttcta
    10261 ttatttcatt caaacactta gagcattcct tacttccaat ctcaagaagg tcttccactg
    10321 aagaatcaga aatctcttct tcattaagaa catgatgcca ttttcttctg ttaagattaa
    10381 gaacatcaat gtcttcttta gatttttcca cataacatct tcttggcaga ggcaaaatgt
    10441 ttataaacca ctttttaaga ttttttggat caaaaagtat tttatggatt ctcttcatta
    10501 gaacactgga attgtatacc tgctttttag atatagcatt aggttgtgtg aaattaataa
    10561 attcagtaat atatcgcact atttcatatg gtagcggatc ttcccctata agctctacta
    10621 tattctgctt gtacgcatca acatctgtgt attttttcca aagccgaata tccattatgt
    10681 attcaatcat atagtggcta aggtgtgctt tttcctgttt tttctcaaaa taaccacatc
    10741 cttgctccct ggtagcttca acgtaggggt gaatagtctt atcgcaataa aagtgtgtta
    10801 aaaatcctgc gaaatatgca agagcttgtt catattcctc tccattgagg ttttccagcc
    10861 atagaacata atccttaaaa aacttagctg tttcaattct gtgcatcatt gctcctgcag
    10921 acctgaccct gttatccaca agcaacgggc tggcatgata aaacaggaag tccggcccct
    10981 gagctccaaa attataaatc tggatatttt tctcaatagc ctctttaaca aaaacatcac
    11041 taatggtgtc tttaactttt cttgcaaaaa ggatatgtgt cagtacatca ggcattaact
    11101 ttttcccact tccaatattt ctatatgctt atctatgtgc gtgaacccag tataatatat
    11161 tgtaaatgag ttcatgctct tttgtacaga gcctctcatg atattatgat agatatggct
    11221 gttatgcgta ttagttatcc taagtgtgtt attatctttt tgtatatcta tatctgcatc
    11281 tttaaaggtc atatgttttc cggcttgtcc ttcatattca ttattctgat tttttactga
    11341 acaaggcgcg ctaaagcaaa actcaacctt atacggaacc tgatccattc cttctgttct
    11401 tatgtctagt tcaactttag agtcgtgcaa agtaatttta acatgatatg ttaattgaac
    11461 ctgatttata attggtcttt ttgaatggtc catctctttc cattttgatg tttcaggctt
    11521 atccggtaaa gggccacgat atcttccttc gcctgtcata gtcatatagt atgtatcatc
    11581 atctattttc ttgatgtctg caggtataaa ctgtgcaaca gcaaaaaatg aagcacatat
    11641 tctcacaaaa catcttaagt tacctttttg aataaaaagg aaatttggag atcttgtaag
    11701 cagtgtaact gaaaaatcat cattcttcct tctgactata tccttatgaa atatgttgaa
    11761 agttatagga cgatcaccta acacaggttc atatgacata agttcagggt taagaagaaa
    11821 cggccataat atgctcggtc ctctgtctac cctgctcttt atattcctga atatatcatc
    11881 agccattctg gcaaaaacag gattcttaag cagataagac atatacatgt atgcatgata
    11941 aaatccatca ggataaaaag atgcggatac atccccttcc tgtttatctt ttctgtttga
    12001 gttttgtgta aacatggtaa aatccggttc aatgtaatac agcatcagtt caagatttct
    12061 tcttacgcat tcaagatagt aattatcctt agtctcttct gcgattatta tgaaagcatt
    12121 atcacattct ctgttataca tcggggatct ttcagtatat tcaccaaatt catcaatatc
    12181 cacaccctct ttaagatagc catatgcaaa gtctgttaat cctttgtttt tgactatgct
    12241 atacagtatg agagcagaag atgagcccat ccacctgtga ttaggcgtta ttatacctcc
    12301 atttgatatt cctttagcag gaaattcaag gtattctcta agtctgtcct ttaaagctat
    12361 ctgttctttt gtttgtgcat atttttccat tatcctataa gcccttgaaa gatatatgct
    12421 taccaaaatg ggaggagcac agtagtcgct ctctcggtta tccaccgtaa aatccggcct
    12481 gtgagctgta tagagataat caatcataca gttcataagg tatataagcc gggaatcact
    12541 atgatatata ctgtcaacac aataatatcc tgaagctatt gtttctatag gaacactgtt
    12601 attggcataa tccggatagc catatgcccc gtacatgact ttttcttcat aattgaatga
    12661 cttaaggtag tgttctattg cagattcgtt tcccttaact atgtctttat aatgagactt
    12721 catcttttat gcttcctttg ttcctattat atcaattttt ttatcgatat gtgtaaaacc
    12781 agtataatat acagtaaagg cacctttact tggaggaatt gaacctctca tatccttgtg
    12841 ataggtatga ttacagaaac tgttagtaaa tttaagagcg tcttgaccct ttgatattaa
    12901 aatctcttca gcgtttacac aaagattacc atctggctca ccggctataa tctcattatt
    12961 aaatttagct gttacaggag cgcttaagca gaattcaatc ttcatcggca cattgtcaca
    13021 ttcttctgtt ctgacattta aaactgctct gtcattttca atttttacgg taacagtata
    13081 ttctaaatca atcatattat ttacaggcct taaggaatga tccatctctt tccattttga
    13141 tgtagaaggt ttttctgaca ttggaccgta atactcaccg tgagctttca ttttcatctg
    13201 atatgtatta tcttctatct tatttatttc caatggctta aactgagcta ctgcaaagaa
    13261 agatgcgcat atcctgacaa aacatcttaa atcgcctttt tgtacaaaca aaaagtttgg
    13321 tgaccttgtt atgagcgtcg cagaaaaatc gtcttttttg aatctaacaa tatctttatg
    13381 gaagatgtta tatgatgttg gataatcttc tagtaccgga tcaaaatcta aaagttcagg
    13441 gtaaagcata tacatatgca aaagtccagg acctctgcca tgtttttcaa caatactgtt
    13501 atatatatca tctgcaaatt tagcatattg tccattctta gttatatatg ccatatgagc
    13561 atatgtataa tacagataat ttggaggtgt tattgcatta atatcccctt cgtgcttatc
    13621 ctgtctgttg gagttctggg taaatacagt gaaatttggt tctatgtagt taaacatgag
    13681 atccatattc ttctttgcat attcaagata ttccattttc cctgtttcca aagcgatttg
    13741 cataaaggat gtatcacata cttcattgta atgaggtgac ctttcagtaa attcaccaaa
    13801 ctcatccata tcaatacctt catttaaata tgcttcaccc attttcctta attcatcact
    13861 ttttagaata ttagccatca tcatcattgc agatgtgcca atccatctgt gatttggagt
    13921 gtgaattcct ccattaagca aagatttttc cactttggga agatactctc tgtaccttct
    13981 tagaaaatcc ttttccctgt cagtctgagc atatttgtca attattctgt atgctcttgc
    14041 catatttatg gcttcaaata acggagcgca gtaaaaattg ctttccatca tatcaatagt
    14101 gtaatttggt ctgtggagag aatcaatgaa tgttaaaaca tcattcatta agtctaatac
    14161 ctgactattt ttatagtgtt tgctgtcact acagaaatat ccagaagcaa gcgtgtccag
    14221 tgaagtgctg ttagctccat aacttgcata accataacca ttcttgattg ccttaatctt
    14281 ttcatcaaac cgacttaaaa gccaatctat tgatgcctca ttgcctctta cgtttcctaa
    14341 ataatgtttt ttcataaaat cactcgacga gcattgctgc tcctacgata cctgcatcat
    14401 tacccatttg tgcgattact aatgttgatg caggcgtttc ctcccttgta tatttatatt
    14461 tttctatata ttctttcaat ggattcaata atctttcacc ctcattgcat acaccgccgc
    14521 caatagcaat aacctgtggc tggaatatgt ttattatatt aacaagtcct tctccgacat
    14581 attttatata tgtatcacat acgtcttttg caaccttgtc tcccattctc tccgcttcaa
    14641 aaggtgttct ggcactaata ttgtctaaat tattattgca tgccttccat atgagggagt
    14701 ctttgtttaa aagagcagct ctctttgtct gagatataag agcagttgca gaagcataag
    14761 cttcgtaaca gccatttctt ccgcatgtac atgcttcgcc gtccattacc agtgtatgat
    14821 gtccaagctc agcagcggca aaattaaaac cactataaac tctattgtca ataaccactc
    14881 cgctgccgac tcctgtacct aaagttattg taactgaaca tgatgtgttt ttacttgctc
    14941 cgcatacact ttcagcaagc gctgctacat ttgcatcatt atcaatatat accggaagat
    15001 taaaatactt ctgaagctct ttgcgtatat ttacattcct catgcctaaa ttattgttat
    15061 atattaatat gccatttgtc ttgtcgggag tcccaggaca tcctacgcca atcctgctaa
    15121 catttttttc atcaatgttt gcttcaccaa gcagtctttt acataaatca gccatatctg
    15181 caattacagc ttcatatcct cgttcacttt tggtaggaac tttgtctttt attactatgt
    15241 tccctaattc atctactact ccggctttta ttgccgtacc accaaggtct attccaatac
    15301 tatacataaa aagctccttt ataatgcata ttgcagcaca agggcaactg caacaaataa
    15361 aatatatact ataaagatgg acaggtcaat gcttgtatat tttaattcac tgatgcttgt
    15421 cctaccttct gaaccatgat aacaccttga atccatagct actgctaaat catcagctct
    15481 tttgaatgac gatatgaata gaggtatcag aatagggata aagctttttg ctctttggaa
    15541 tatattccct gttcctatat ctgcacctct ggctgcctga gctttcatta tcttctcagt
    15601 ttcttccatc aatgtaggta tgaatctcaa tgctatggaa atcatcatag ctatttctgc
    15661 tgtcgggact ttaatgtatt tcaaaggaga catcaatctt tctataccta ctgtcaggac
    15721 caaaggagta gtagtatatg tcattagaga gcctgctgat attaaaagaa gaatcctgac
    15781 gcttataaga gctgatgtga ctaatccctc ttttgtgata gttataatcc agaatgtata
    15841 caatggtgtt ccagtcgcaa agaaaatatt taatatcatg gtaaagatta ctatgaatat
    15901 cactggcttg agtcctttta gcgcatatct tagacttact ttagacacta aaaaggctga
    15961 tacaacaaaa atagcagcta ataacaatgc atagaatgaa tcaagaatca gcaataaaac
    16021 cattgttaat atggttattg ctattttcag tcgtgggtct gctttatgca caaccgactc
    16081 acctggtatg tattgcccta ttgttatgcc ctgcacttca agcctcctta ccaatccgat
    16141 agattatatc aaaaaaatca tggattttac aacttaagtg cattctgatt ctttttttct
    16201 ttcttttctt aaaatatatc ttttctttcc catttcccaa tcacatttct cttcttcagt
    16261 ttcaataata agacctggaa cttcagctgg tctctcaaac tcatccaatg caaccataac
    16321 gacataagcc tcatttatga gcttcttttc cccgattaag tcttcaacat atgatttaac
    16381 gcatacctcc attgaagtcc ttccaacata tgtaactttc gcagtaagaa gaacagtatc
    16441 atttgcatat gcaggtcctt tgaactccaa tgaatccact gctactgttg taacatttct
    16501 gtttgaatgc cttcttgctg caatagcagc tgttacgtct atccattcca tcatctgccc
    16561 tccaaacagc cttgaattac cattaagagt acattgtgta agtatctgtg cttgttcagt
    16621 atatgagtcc ttaacatgtt ttttcatata tttctccttc tttatgctag tataattgct
    16681 caacagcaat agttttaaaa tacaacatct gtttattttg tcttactgtc attatccctg
    16741 ctttcgtaat acttaaatta attatatccc cttcaggaag tttctgtatc gttatcttgt
    16801 cgactaaact atccagcgta agccatcttt ttgacatctc gctcattata tatttctttt
    16861 ttcccgtttt tacttcagta actctgttaa agatatcttt ttttacaagt tcgtctatat
    16921 cactaataat attatcggta tcccagaaat tcttactgat ctcgtatccg tctttatctc
    16981 cgagtattaa tgctgctgat gatgcatcta agatattctt tctgttgata aattctgaga
    17041 tactcattgc tgattcatct ttattcagag gagccccctt ttttctgact ttatcaacca
    17101 gtgcaagctg cattacttca aatataatca gttctccaac tgtcatggaa acatcaatac
    17161 ctaacccctc ttcaaaatcc atatcgctta aaaaatcttt tttaattttc atacctaagt
    17221 ctttgtttac tataggtgtt tctattgtga taattgtgtg atctatattg ggcagaattc
    17281 ttacccagaa tccattagca taacaataat gcgcagttgc catatcaatt gcattacaac
    17341 taacaacact tattacactt tccggactta atatagcttc agcaataagt ctgcccgata
    17401 gtgtgatggc tcctttttta actaaaccaa gttttgccag ttcgtttttt gccaatgaca
    17461 aagaggacct attatttata tctggattaa aaacagcttg tgatatctta ttctgttcaa
    17521 tacctgataa gttgatcagt gctgtataat catatgaact taagatatag tacatatcgc
    17581 ctcctaccag tcaaacagac tttttacaac taatgaggtt tcctcatatt tgaagttatg
    17641 tgtctgtgcc tgctttactg ctcgttttgt tgagtaaact ctgtagcttg atatctcttt
    17701 atactcctct tcagtaagag ttccatttat aacactctca tatagtttat caagctcatc
    17761 tgattttata accgtttgag taaatttctg gacagtttcg tatgttattt caccctttct
    17821 cgacatattt cttattttat cgccatatct tccatttaac gcatttctat atgctgtttg
    17881 cgcatcattt atggaatcca gaccgattga cattataccc cgtaaatgaa ctgaaggact
    17941 tactaaaact cccgctttca tattctccag agcaaacgca ttggcaagtt cgataaaagc
    18001 acagtaggga tttcccgatg caagactgtt gaatacataa tttgaataaa cgattttacc
    18061 agttgcattt gttatttctt ctcctcttat atccagcgca tgtgcatcat tattataccc
    18121 cagagcagaa aggtcatgga ttaaccctga aacactgttt ctatatctgt ttattgatga
    18181 ttcaatagca tatatgctct ttgactgaat tatatggtct ttgctctttg ataatgtttt
    18241 ttgaaacact tctttgtatt ttggccatcc tgcagccgaa gcttttgaaa gagaatcata
    18301 cagatttgta agattatcag ataaatctga ggttgagata ttattatcat atcctgcatt
    18361 ctttcccttt tgtataatat cagtcttctt ctgagttgtt ttactcgtgc ttttttttct
    18421 tctaagagtc aatgagccta ctgatacagt tgctcccatt aaagctgctg ttatagttat
    18481 aaagttagtt ataaccgtac cccatggctt gtaatgctcg ttatcacttc ctgaacctgc
    18541 aaaccatgtg aaaaactggt cagtaggtga tttctgcaaa ttataaacgt attcaaccct
    18601 gccaccaaat gatgcctgaa ctgatatagt aagcctgcag ttattcttgt ttagttcttt
    18661 atataaatca taataattca atattttcaa gaagaaagcg gtataatcct gctcatgagc
    18721 taaaaacata ttttcagttg caaaatcctt actagcttta aaatattcgt taatttccgg
    18781 atttttgtca tcgggtatgt atgttataaa catcgaagtc tttaatgatg atgtctgttt
    18841 cgtatcttca ctcccgctgt acactacttt ttggtttata agcagttttg tctcattacc
    18901 tgcataaatc tttttaggag ggatatccca gaaatattca atattgtaag tagcatcaac
    18961 tgaataatgc ttgcgaatgt acccatcaga cacatatgta ttctcttttg tattttcgtt
    19021 tgtatctgga tcaataatat ttatatcgtg caacacccat atgcctttta tctcatttac
    19081 cggagcagct aatacgctga ttggagaaag cagtattatc gtggcaagta taataggcat
    19141 tatgcgtctg aatcctgctc tgcgaataat taggacatca ataagcctta taacaagtga
    19201 tatgaatatg agtaataacc ctatgtaata acatataaag gctgaactga atagaaaaca
    19261 tggtatggcc agtaaaatac cgactatata tcctccaagt gatctaatag taatatcttc
    19321 agacatagat tttccgaata ctctatggaa gatatcccag gtaataccgg atttcctgcc
    19381 aatggcacgt aaaatgataa ttacaatcac aggagaaata atgctgttta tccaattgtt
    19441 atttcccgtg aacatattaa agccgataaa tgaaagacct gctgtcagaa ccggaatata
    19501 tatatcctta agatttttaa aacgcaacat gctgttaaaa aacatcctta aacctgtaga
    19561 aatacccttg aaaggttttt gcaaagaata aacaggagta agaagcatca caatgaaata
    19621 taaataaatc atctttccaa caataccacc gacagctcct aataaccctc cggacatagc
    19681 tccatctgaa tatgttaaaa aagcgaagaa atttaataat ggtgttcgaa tttctaatgc
    19741 aaatagcaaa gatataacca gccagacagc agctaaaaca tatactggaa tcaaataggt
    19801 cgctcttctg attccttttc tgaaatccat caatatagac ttaatgcctt ttaaacccag
    19861 tcggctttta ctattttccc atggtttact gaattgaacc atattttttg aaaaggagta
    19921 gtcagaaaca ttggtaatac caattggtgt tccacatttt ctacaatatt tagccttatc
    19981 tgaaacatgt aatccacagt tcggacaaac ccgggccatt tgctttcctc cttattcaga
    20041 attgatttta tcataatata ccgagtattt acacataaag cggtttacaa tataataaac
    20101 aaacagaaca ggaggccaaa tggcatcacg cacaaagcaa atcgcataca ctggaatgtt
    20161 cacggcaatt attgctgtta tgtcccagct tgcaattcct atgccgtcag gtgttccagc
    20221 cacactgcag acactggcta tcgtaataac cggtcttata ctcggtgtaa aggctggaac
    20281 tgtatctgta ggcctgtata taactatagg agctataggc cttccggtat tttcagcctt
    20341 cagaagcg
//
LOCUS       DAOSXM010000034        19582 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2111
            NODE_1756_length_19582_cov_12.019358, whole genome shotgun
            sequence.
ACCESSION   DAOSXM010000034 DAOSXM010000000
VERSION     DAOSXM010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864178
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 19582)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19582)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:24:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,261
            CDSs (total)                      :: 2,229
            Genes (coding)                    :: 2,218
            CDSs (with protein)               :: 2,218
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 3 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19582
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1756_length_19582_cov_12.019358"
                     /isolate="AMR_MDS_2111"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            54..809
                     /locus_tag="PLQ68_05930"
     CDS             54..809
                     /locus_tag="PLQ68_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006566755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HXK72224.1"
                     /translation="MKTYTTSKAAAIIGIHPNTVRFYEKAGLITKAIRKPNGYRIFTE
                     LHINQMKLLRIALRAEVLQNGLRKKAIEIIMKNAELDFSGSIESSTMYIYMIKNEIEQ
                     AKKAISSVENILNNSRPSVRISLKRTEAAKYLDVTVDTLRNWELNGLITVKRKQNGYR
                     IYDENDLRILSIIRTLRCANYSLSSILRLITSQHSKTCSNIEQILNTPLADDDIISAC
                     DKLITSLENTLSDAYEINKMAKNIELKFSNPTI"
     gene            869..2071
                     /locus_tag="PLQ68_05935"
     CDS             869..2071
                     /locus_tag="PLQ68_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011458812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXK72225.1"
                     /translation="MENWKKKTILFLASQAITLFGSSLVQFAIVWYITLQTSSGVWVS
                     ALTICSFVPQFIVSFFSGVWADRYSKKMLIILSDTMIMLSTLALAIIIPHIGKDTTVF
                     IALLVTSVIRSIGAGIQTPAVSSMIPLLVPQKNLMKFNGINATVQSVIQFSAPAAAAA
                     ILSFKTLTSTLLIDVATGVVGIGILSLIAIPKQIFDKDALVVSVFSEMKSGMKYAFSH
                     KFIYKILIIFGLFIFLSVPAGFLAALFVSRNYGDSYVYLSIVEIVGFAGMAGGGLLIG
                     IWGGFKKRSLTLFTGLLSFTLLTIAMGVIDSFIVYLLLMAIYGIALTMVQTSITTIIQ
                     ENTEESMQGKIFGFLNAMYSGCLPIGMAVFGPLSDVVSLRLLMIITGSLMAIIAFFVN
                     YDKSIKSN"
     gene            complement(2074..2904)
                     /locus_tag="PLQ68_05940"
     CDS             complement(2074..2904)
                     /locus_tag="PLQ68_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004629195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72226.1"
                     /translation="MKVYALVGPSGTGKSHKSTLLASDMGITHIIDDGLLISENRVIA
                     GTSAKKEATKIASIKRAIFDDNEHRKEVVKAIKSSKADSILILGTSDKMVNEIAQRLE
                     IPSVQKIIRITDISDEEEIAIATKSRQEKGKHVIPVPTFEIKKDFAGYLLDPLYIFRR
                     RDKSKYEPDGTKTIVRPTFSYLGGYTISNNTLFQIANYGCTAINGFEGMTRFRVINRP
                     EGIALEVNITANIDYDILETLKMVQQKIYNVLDHATALNMLRIDVTVKNVMINGEVFP
                     "
     gene            complement(2968..3606)
                     /locus_tag="PLQ68_05945"
     CDS             complement(2968..3606)
                     /locus_tag="PLQ68_05945"
                     /inference="COORDINATES: protein motif:HMM:NF017026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HXK72227.1"
                     /translation="MTDHYYTDKPISKNNVFRYAVNINEVQFQFFSNSGVFSKQGIDF
                     GSRLLIDTVLEDADMIESGLSNEEKPSLADLGCGIGVIGIVLNRLLKINSTYLYDINE
                     RAITLAKMNIDNNASKRTYAIKKDIVNEQILERADICVSNPPIRAGKKTVFAFYERAY
                     ELLNKNGTFYCVIQKKQGSESTKNKLNDLFGNSEIIQRSAGYHIYKCVKNCD"
     gene            complement(3679..3849)
                     /locus_tag="PLQ68_05950"
     CDS             complement(3679..3849)
                     /locus_tag="PLQ68_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004619190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HXK72228.1"
                     /translation="MPYRITDECISCGACEAECPVSAITQGDDIYVIDAELCISCGAC
                     ADVCPVDAPIEE"
     gene            complement(3948..5138)
                     /locus_tag="PLQ68_05955"
     CDS             complement(3948..5138)
                     /locus_tag="PLQ68_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016208138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HXK72229.1"
                     /translation="MLLSIALIFLCALLLSGILIKFKLPGLLGMLIVGIVLGPYVLNL
                     LDDSILNISADLRTIALIVILLKAGLSMNLNDLRKVGRPAILMSFVPASLEIIAFIVF
                     APMLLGITYLEAGLIGAVMSAVSPAVVIPKMSKLIDEGYGTDKAIPQMIIAGASADDV
                     FVIVIFTSLISIATGGSFSISDLTKIPVSIVLGLIGGIGIGFILVWLFKKYHMRDTIK
                     VLILISISILFMPLEDLLSGIIGFSGLLAIMALGIIIFNRYPVLAQRLSGKFVKLWVA
                     AEIALFVLVGSEVDISYVVNAGFITVVLIFIGLIFRLAGTYLCGIKTQFNSKERLFIM
                     ISQIPKATVQAAIGAIPLSLGLSCGDLVLTFAVVSILITAPLGAFLIDVTHKKLLTGI
                     NKQI"
     regulatory      complement(5172..5259)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            complement(5297..6184)
                     /locus_tag="PLQ68_05960"
     CDS             complement(5297..6184)
                     /locus_tag="PLQ68_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012157948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HXK72230.1"
                     /translation="MVTVVGIKFRQSNKIYSFAPGDWELNIDDLVIVETVRGIEMGTV
                     ASEIAQVEESKIIAPLKEIVRKATADDIAKAEDSKNKEKEAFAICEKKIQDHKINMKL
                     VGVEATYDMSKILFYFTADGRVDFRELVKDLAGTFRTRIELRQIGVRDEAKLLGGIGI
                     CGRPFCCKTFLNDFQTVSIKMAKDQAMSLNPLKISGVCGRLMCCLKYEHEVYTDLIRK
                     TPGIGAIVKTNDGKGEVIDVSMLKGELKVKLDKGEEIDIRTYNAQDVEVLKRSSRYKV
                     LPSDDEAVDDNELSKLEDK"
     gene            complement(6178..7134)
                     /locus_tag="PLQ68_05965"
     CDS             complement(6178..7134)
                     /locus_tag="PLQ68_05965"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta' C-terminal
                     domain-containing protein"
                     /protein_id="HXK72231.1"
                     /translation="MTGFDDIKGRKPLKNQLKKLIDKGRISHGYIFEGPKGSGKKLIA
                     DAFAELLFCYNQKGLETCKTCKGCTGYKEKYVLETEKSISVDDIRSLVADTELMPFAN
                     NKKIYIIHDADTMTDQAQNAFLKTLEEPPPYAFFMLTVTNSEQIIDTIRSRCIILYTG
                     VNTKQEVLEYLEQNNPDADTKSIYAAACFAEGCIGRAADLINDQSFIQCRKTAVDYIT
                     KSQQDFDIKDLKGDVLVFLEILQSLFRDMLVYKATNDSSNLINTDISDIIKRTAERNS
                     SKSLLIAFNSAMKASEYIKDNVSQSIAIGTLNIELMEELNIW"
     gene            complement(7136..7465)
                     /locus_tag="PLQ68_05970"
     CDS             complement(7136..7465)
                     /locus_tag="PLQ68_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HXK72232.1"
                     /translation="MKLIFAIVNDEDELDVMRGLSQEGYGVTKLCSTGGFLKSGNTTL
                     LIGVDDEKMDHAIEIIKTKSKSRKKVVNSSMIGSSQGAVLTGYPIEITMGGATIFVTD
                     VVKYIKE"
     gene            complement(7466..8095)
                     /gene="tmk"
                     /locus_tag="PLQ68_05975"
     CDS             complement(7466..8095)
                     /gene="tmk"
                     /locus_tag="PLQ68_05975"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HXK72233.1"
                     /translation="MAKGMIISFEGTDGSGKTTQIKALSEYLVNKGYKAQMLREPGGT
                     KIGEEIRHILLNKDNTEMASLTEMLLYASSRAQMMEEKVQPLLNEGYVVILDRFFDSS
                     LAYQSYGRNLDFDAVLAINLAAVKNIIPKITFFIDITPEQSLKRRKNATETDRLENED
                     MSFHYKVYEGYKQLLKRFPERIIRIDGTETPKEITKEIIKYVDIIMKEE"
     gene            complement(8103..8909)
                     /locus_tag="PLQ68_05980"
     CDS             complement(8103..8909)
                     /locus_tag="PLQ68_05980"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015617531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HXK72234.1"
                     /translation="MLLAIDVGNTNIAMGVHNGKSYIDVWRLTTNKERTADEFGSFYL
                     NMFNAAGLKLSDVDAIILSSVVPGIMYTLTHSLEKYFNLTPILVEQGIKTGLNIKIDN
                     PRELGADIIVNLVSAYETYKGPAIVIDFGTATTFSCVTKDFECPGVVICSGVKLTAEA
                     IYKRAAKLPNIDIVMPPKIIGKNTINSMQAGIVYGYLGQVANIVKHMKKELVEDYNQT
                     YEDIKVIATGGLLNLIAKQSEYIDYVDSMLTLNGLKMVYDKNKPRIEGKA"
     gene            complement(8911..9270)
                     /locus_tag="PLQ68_05985"
     CDS             complement(8911..9270)
                     /locus_tag="PLQ68_05985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72235.1"
                     /translation="MTENQNVTSNSDNNQSKPRKKNNNYYKKNKNRPYKKPYNNTQQA
                     QSKDCICPEDTDNADKDINTQKKAYSKNYNSYKRYRKDKRNGRDIVETVDDVKKDIQR
                     IEKEIRLEIEEIKMIRI"
     gene            complement(9272..10024)
                     /locus_tag="PLQ68_05990"
     CDS             complement(9272..10024)
                     /locus_tag="PLQ68_05990"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HXK72236.1"
                     /translation="MQVIKYISLESIDSTNTYAKKNAKLFDDEFTVIQSKIQLSGRGR
                     MDRVFISDNEEGAWFSLVIKPKRNEVSPKQSLLIPIMAALAVAVSIEDLYKIKCGIKW
                     PNDILINYRKVCGILCESQTVNDSINYMIIGIGINVNQSSFNEQIKDIATSLIIETEK
                     NLPVDLLIQNICGNLLSLYDKIKYGKTACIIDKWNQYSLMDNTVITYTKEQLSYTALS
                     KGIDSTGRLIIEQDGEINYLDSNEIRLKIQGA"
     gene            complement(10026..10607)
                     /locus_tag="PLQ68_05995"
     CDS             complement(10026..10607)
                     /locus_tag="PLQ68_05995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72237.1"
                     /translation="MITYHHVVNGKVKDATTFGMKLSEYGDNSIKTGNGFKSVIVAYL
                     CPADDIKRYKLPNYTCLSLDVSAEKAFIVEGTYLDMDNQKALGDSLIRASEYILGTYR
                     KPIVLITTTVFSEKIKVLGKYMDIPMIVDDSEELYLKSSLTDLENADSRYFEKALSGY
                     FLVSADARKIGEKNGFIEYLLNGRKYIVRKTGK"
     gene            complement(10604..11695)
                     /locus_tag="PLQ68_06000"
     CDS             complement(10604..11695)
                     /locus_tag="PLQ68_06000"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HXK72238.1"
                     /translation="MEIKSYNPVRIIFGIDCVRNNMDVFKGMGRFPLIVTGRSGAKKS
                     GAFDDVIDVLKKGPYVLYDRIEENPKISTVEDAAMMSKKFNCDYIIAIGGGSALDAAK
                     AIAVLAVSNMSGMDLLSSKIEKALPIIAIPTTSGTGSEVTPYSILTVDQWQTKKSFAS
                     DLVFPKYALQDPKYTYSLPDDYTVSTAFDAASHLFESYFSLRSTNISMMYSLEGIKAF
                     EKAVKAIDELNFDEESRYYLMYSSMMGGLAITHTGTTAMHALGYSMTYFHNMPHGFAN
                     LYFCRPYFKHMLKHEIQKTLHIVAQMGFTDVGSMCNFFLRNMERPRLDKSMCEKYASL
                     AMLQSSIKATPGELTEADIVSMYKEAGSL"
     gene            complement(11685..12278)
                     /locus_tag="PLQ68_06005"
     CDS             complement(11685..12278)
                     /locus_tag="PLQ68_06005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72239.1"
                     /translation="MRQHKKTTIAVLAVVMIMSLMFCTTAQTADLVIASVEYVDAKIA
                     EVKALIQTSGSTGGTTVNPVDNAKITELETALGNALKEIDALRARMDFLDITVRDISD
                     NYGIQVLHVYNGDIIYALGTSELILRTGSAVVIGNENNEGLSDNTSGKSLAPGASLEK
                     DHFVIIPRGDGRGIKITSGDSYIMYRGSFSYVPADGN"
     gene            complement(12359..13504)
                     /gene="alr"
                     /locus_tag="PLQ68_06010"
     CDS             complement(12359..13504)
                     /gene="alr"
                     /locus_tag="PLQ68_06010"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HXK72240.1"
                     /translation="MKKPDRVWAEINLDHIAHNISSIKEKAGDVKIAAVIKADGYGHG
                     AIEIAQTLCENGVYMLVVATLDEGINLRINGITAPILVLGYIRPDRLEQVIENNLAAT
                     ITNLDMAKNFSNTAEKLEKTLYAHIKVDTGMHRVGFSYDDKESLTTALGLKNIHYSGI
                     FSHFSTADEEDLSYTDLQYKRFLEMLESIKEEGFDIALRHICNSGAVILHPDKYLDMI
                     RPGIIIYGLYPSQYCKEHDGLKLKCAMTFKSRIIQIKTVRKGQPISYGNKFVTDKDSV
                     IATIACGYADGYSRVLSQKANVYINKQTAPVVGNICMDMCMADITDLKGVSENDEVEL
                     FGPNIAVEDLADICSTINYEYICKIGIRVPRVYIKEGKILKISNGLI"
     gene            complement(13491..14336)
                     /locus_tag="PLQ68_06015"
     CDS             complement(13491..14336)
                     /locus_tag="PLQ68_06015"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HXK72241.1"
                     /translation="MKIVEYSKQDISAFFQLRKAQSNKSDYGKFLVIAGSIGMTGALR
                     LCVDSCYRSGAGLVYYTAAKKHMMEYDALTVEGITVPLEDIKEHIQNKDCICIGCGLS
                     LNEQNQLLLKRVLELSKCHVIVDATALKMIALEKDLLKRYKGRMTILPHPGEMSVLMG
                     MSIDEIQKDRVKTAYSFYQEYGCITVLKGHETVVAGNDMVYINKTGNAGMATAGSGDV
                     LAGMTTAYSVMTDDMFRNTCMAVYVHGLAGDLAVKDLGMISMTASDIIRYIPNAHKEV
                     AGEKA"
     gene            complement(14413..14874)
                     /locus_tag="PLQ68_06020"
     CDS             complement(14413..14874)
                     /locus_tag="PLQ68_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small multi-drug export protein"
                     /protein_id="HXK72242.1"
                     /translation="MLQQYLYILGISAIPIIEQRGAIPIGILVYNLDPVLVTLVSYVG
                     SLIPMFFIIFCFSPIFKWLGTKKILKWFYDFVDKKVTKGQKKVEKYETIGLSLFVAIP
                     LPVTGVWTGSAVAAFMQMNKKKALISVSVGAAISAVVIAVICYVAPKLLGY"
     gene            complement(14900..17233)
                     /locus_tag="PLQ68_06025"
     CDS             complement(14900..17233)
                     /locus_tag="PLQ68_06025"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF024703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HXK72243.1"
                     /translation="MQKKINVSVRDIADYSLNKGDIETHAGFSYKEAAFEGTQNHIRF
                     QKEMIKEHGKESFTKEVYIDDLAENEEISMNITGRIDGLLKTDTQDGINTYIYEIKTT
                     NRDISEVKPSDKPEHLGQAQCYACMYMKKHGLESIGVRLVYINRENQDSITFEYSYTY
                     DEIQAIYSDLVLRYLNWITGIRKWQIIRDQSIERLEFPFSSYRNGQKRLMGEVYNCIN
                     DSIKLFITAPTGIGKTMGVMFPAIKALGAGLVDKLMYLTAKTVTAKVAVDAYKRLEKN
                     GLNLRTVCISAKDKVCPLEKRDCDPEKCPRANQYYLRARQCLNDMLHEQFFDKDIIAR
                     YADSYNICPFELSLDLSNYCDLIICDYNYLFDPRIRLQRYFDYQNEQAFCFMVDEAHN
                     LVERGREMFSCTLNLKQMKLFKDSVKKEWKVLRKNVNAVIAVMNSLKKEHFENMNEGF
                     IYQEQYPDNLVEEVNKCLNSIMMYDFKKIPEDKREDFLDGLFNLLYFARVADFYDKRF
                     VTLYSDNTKGLDIKLMCLDPSYLLKEGMDMGRSSILFSATLEPRRYYMDLLGGDADVD
                     SYISLDSPFPKENLRIKLVTDISTKYKDREYSYEKIAEILKKEFSRKTGNYMVFFPSY
                     AYMKRVMDIFTVIYPDAVVMEQKPYMDDIDRAIFLNRFEQFGDNTLVAFAVMGGLFGE
                     GIDLEGEMLSGAAIIGPALPMVCPERELIKKYFDESDMNGFDYSYTYPGMNRVLQAAG
                     RVIRSETDTGFVILIDSRFGTSKYKSLFPKWWNYSNI"
     gene            complement(17307..18032)
                     /locus_tag="PLQ68_06030"
     CDS             complement(17307..18032)
                     /locus_tag="PLQ68_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HXK72244.1"
                     /translation="MSGHSKWANIKHKKERSDSSRGKLFTKIGREIMVAVKSGGPDPN
                     ANSRLRDVIAKAKSNNVPNDTIAKSIKKASGEGNAENYDQITYEGYGPNGVAVIVETM
                     TDNRNRTASDVRHYFDKFGGNLGTNGCVSYLFDKKGVIVIEDDGTIDADQLMMEALEA
                     GAEDFENEDEMFEITCDPAEFSALREALETKGYNFLQADLQMIPQTYVKIDEDKVASM
                     EKLIDNLDDLDDVQNIYHNWEQE"
     gene            complement(18034..18672)
                     /locus_tag="PLQ68_06035"
     CDS             complement(18034..18672)
                     /locus_tag="PLQ68_06035"
                     /inference="COORDINATES: protein motif:HMM:TIGR00257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HXK72245.1"
                     /translation="MAQPGSDNIIYTIRKNAESELIEKKSRFIAYAIKTDTEKQALAE
                     LDNIKSRYPDATHHTYAYTINENGVTYQRYSDDKEPSGTAGLPILDVLRKNNIENVII
                     VVVRYFGGTLLGTGGLVRAYSQSAKDAVDKASLARRVSCTIYSIKTEYSSYEKIKRLL
                     NENVILEILYEDQVQVRFAVEDLKAEEEIGKINELINSRCEIEKEKNIYLEI"
     gene            complement(18650..19132)
                     /gene="dut"
                     /locus_tag="PLQ68_06040"
     CDS             complement(18650..19132)
                     /gene="dut"
                     /locus_tag="PLQ68_06040"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004629100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HXK72246.1"
                     /translation="MIKVYIEKVRPDAKIPTYANNFDAGMDICACEDRDIDINSTCVV
                     PTGLKVAIPEGYEIQVRPRSGLSLNTRLRITNSPGTIDTGYRGEIGIIIHNSSQANEG
                     DLYISEKSNRQGIYHIKKGDRIAQIVLQQVPRMEFVETDDVTKIGQDRGGGFGSTGVR
                     "
     gene            complement(19129..19509)
                     /locus_tag="PLQ68_06045"
     CDS             complement(19129..19509)
                     /locus_tag="PLQ68_06045"
                     /inference="COORDINATES: protein motif:HMM:NF017016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HXK72247.1"
                     /translation="MKTTKIRICNNEYLIKGNESEEYIEKIALFVDKKMSAIMRENPK
                     LSVSMAAVLTAVNIADDYFKERDQANKQKAFMDDFERKSKDQVSRMKILEAKVEKLTQ
                     SNNELKNTLSETEEQLKKAQNEFR"
BASE COUNT         5693 a         3993 c         2920 g         6976 t
ORIGIN      
        1 ctgttttttt cgttaccaaa tctactcatt ttgtagtatt ctttattata atgatgaaaa
       61 catatactac ctccaaagca gctgcaataa ttggtattca tcccaacacc gtccgctttt
      121 atgaaaaagc aggactgata actaaagcga tacgaaaacc aaatggatat cgaatattta
      181 ccgagttaca tataaatcag atgaaattgt tgaggatagc attaagagca gaagtgctgc
      241 aaaacggatt aagaaaaaaa gcaattgaaa taatcatgaa aaacgcagaa ttagattttt
      301 ctggttctat tgaaagttca actatgtata tatatatgat caaaaatgag atagagcagg
      361 ctaaaaaggc tatatcttct gtcgaaaata tactaaacaa cagtaggcct tcagtcagga
      421 tatcgctaaa aagaacagaa gctgcaaaat atcttgatgt tacagtggac actttgagaa
      481 attgggaact caatggtctt ataactgtaa aaagaaagca aaatggttac aggatatatg
      541 atgaaaatga tttaaggatt ttaagcatta taaggactct gaggtgtgca aattactcat
      601 tatcatctat actccggctc attacatcac aacacagtaa gacctgttcg aatattgagc
      661 agatactgaa tactccttta gcagatgatg acataatatc tgcttgtgat aaacttatta
      721 cttcacttga aaacactttg tctgatgctt atgaaataaa taaaatggca aaaaatatag
      781 aattgaaatt ttctaaccct acaatataac accagggttt taaaatagtg taatatcctc
      841 tttgtttaca aggaggtaat caataaatat ggaaaattgg aagaaaaaaa ctatcttatt
      901 cctggcaagc caggctatta ccctttttgg ctcttcttta gtacagttcg caattgtctg
      961 gtacataacc cttcagacat cctcaggcgt atgggtttca gctctgacca tatgctcttt
     1021 cgtaccgcaa ttcatagtgt cattcttttc cggagtatgg gctgaccgct actctaaaaa
     1081 aatgctgatt atactgtctg atactatgat tatgctttca actctagcct tagcaattat
     1141 aataccgcat ataggtaagg acacaacagt atttatagct cttcttgtta cttcggtaat
     1201 ccggtctatt ggagctggca tacagactcc cgctgtgagt tcaatgattc ctttacttgt
     1261 gccacaaaag aatttgatga aattcaatgg cataaatgct actgtacagt cagttattca
     1321 attttcagca ccagcagcag ccgctgcaat actatcattc aaaacattaa cttctacttt
     1381 gttaattgat gtcgctactg gtgtagtcgg cataggaata ctttcactta tagctatacc
     1441 aaaacagata tttgataaag atgcgcttgt tgtttctgtt ttttctgaaa tgaaaagtgg
     1501 catgaagtat gctttttcac ataaattcat ttataaaata ctgataatat tcggcttatt
     1561 catctttctt tctgtacctg caggattcct tgcagcatta tttgtaagtc gtaattatgg
     1621 agactcttat gtttatcttt ctatagttga aatagtgggc tttgctggaa tggctggtgg
     1681 aggtttgcta attggtatat ggggaggatt taaaaaaaga tcactaacct tatttacagg
     1741 gctgctcagt tttacccttc tcacaatagc aatgggagta atagacagct ttatagtata
     1801 tctattatta atggctattt acggaattgc gcttactatg gttcagacat ctataacaac
     1861 aattattcag gaaaataccg aagaaagtat gcagggtaag atatttggat ttttaaatgc
     1921 tatgtattca ggatgcctgc caataggaat ggcggtattc ggtcccttat ctgatgtggt
     1981 gtctttaaga ttattaatga ttattaccgg ttcgttaatg gcaattatag cgttttttgt
     2041 aaattatgat aaatcaataa aatcaaactg aatttaagga aatacttcac cgttaatcat
     2101 tacatttttt acagtaacat caattcttaa catgttaaga gctgtagcgt ggtccagcac
     2161 attgtatatc ttttgctgga ccatttttaa agtctctaat atgtcataat ctatattagc
     2221 agtaatattt acttctaaag ctattccttc aggtctgttt ataactctga aacgggtcat
     2281 accctcaaaa ccattaatcg ctgtacagcc ataattagct atctgaaaaa gagtattatt
     2341 ggatattgta taacctccaa gatacgaaaa tgtaggacga actatagtct ttgttccatc
     2401 aggttcatat tttgacttat ctcttcttct gaatatatat aacgggtcaa gcagataccc
     2461 tgcaaaatcc ttcttaattt caaatgtagg aactggtata acatgttttc ctttttcctg
     2521 tctgctctta gtcgcaatag cgatttcttc ttcatcagaa atatctgtta ttctaataat
     2581 cttctgtact gatggtattt ccagtctttg cgctatctca ttaaccatct tgtctgatgt
     2641 acccaatatg aggatggagt cagctttact tgactttata gcttttacaa cttcttttct
     2701 atgttcatta tcatcaaaaa tcgcacgctt aattgaggca attttagttg cttccttttt
     2761 tgcgcttgtt cctgctatga ctctgttctc acttattaaa agaccatcat caattatatg
     2821 ggtaataccc atatctgaag caagcaatgt ggatttatgg cttttgcctg tcccgctagg
     2881 accgactaaa gcatatacct ttatagcaaa cctccaaaca tttatatatt ctgattatac
     2941 catatcatat ggactattca gatactgcta atcgcagttt ttaacacact tgtatatatg
     3001 atatcctgca cttctttgaa taatctcact gtttccaaaa agatcattaa gtttattttt
     3061 tgtactttct gaaccttgtt tcttttgtat aacacaataa aaggtgccat ttttgttgag
     3121 aagctcgtat gccctttcat aaaacgcgaa tactgttttc tttcctgctc ttatcggagg
     3181 attagaaaca catatatctg ctcgttccaa aatctgctca ttaacaatat cttttttaat
     3241 agcataagta cgttttgatg cattattatc aatattcatt tttgccaaag taatagctct
     3301 ttcatttatg tcatataaat atgtgctgtt tatcttcaat agcctgttaa gaacaattcc
     3361 tattacacct atgccacaac ctaaatcagc taatgatggt ttttcttcat ttgataatcc
     3421 gctctcaatc atatctgcat cttctaaaac agtatcaata agcagtctgg atccaaaatc
     3481 tatcccctgt ttggaaaaaa ctcctgaatt tgaaaaaaac tgaaactgca cttcatttat
     3541 atttacagca tatctgaata cattgttttt cgaaatcggt ttatctgtat agtaatggtc
     3601 tgtcatatct tccctttctt ctgctaaata tgaaaaaggg cggtcatgcc gccagttctc
     3661 aatgcttaat gtttcctgtt attcctcgat tggagcatcg acaggacata cgtctgcgca
     3721 tgcaccgcat gagatacaaa gctctgcgtc tatgacataa atgtcatctc cttgagtaat
     3781 tgcgctaact gggcactctg cttcgcatgc accgcagctg atgcattcat cagtaattct
     3841 atatggcatg tttcacctcc tatagtttct tatggcaaat tatataactg catattttaa
     3901 aaagcaacaa agataagaca atttacatat atctatttcg aatagggtta tatctgtttg
     3961 tttatgcctg ttaataattt cttgtgagta acatcaataa gaaatgctcc taaaggtgct
     4021 gttataagta tcgaaacaac tgcaaatgtt aacactaaat caccacagct taaacctaaa
     4081 gataatggaa tagcacctat tgcagcctgt actgtcgcct ttggtatctg tgaaatcatg
     4141 atgaataatc tttctttgct attgaattgg gtttttattc cgcaaagata tgtacctgcc
     4201 aatctgaata ttagtcctat aaaaattaaa acaacggtaa taaatcccgc attaaccaca
     4261 taggaaatat caacttctga acctactagt acaaaaagcg ctatttcagc agctacccat
     4321 agttttacaa acttacctga taatctttga gctaatacag gatatctgtt aaaaataatt
     4381 atacccagag ccattatagc caataatccg gaaaatccaa taatacctga aagcaaatct
     4441 tctaaaggca taaataaaat agaaatggaa atgagaataa gtacttttat agtgtccctc
     4501 atgtgatact ttttaaatag ccagactaag ataaaaccaa tacctatacc tccaattagt
     4561 cctaatacta tagaaactgg tatttttgtt aaatcagaaa tagaaaaaga accaccagtt
     4621 gcaatggaaa ttagtgaagt aaatataact attacaaaaa catcatcagc agaagctcct
     4681 gcaattatca tttgaggaat agctttgtca gtcccatatc cctcatctat aagtttactc
     4741 atttttggta ttactactgc tggagaaaca gctgacatta cagcacctat caatccagct
     4801 tctaagtatg taatacctag taacatagga gcaaacacaa taaaagctat gatttcgaga
     4861 cttgccggca caaatgacat caaaattgct ggtcttccta cttttcttaa gtcattaaga
     4921 ttcatagaca acccggcttt tagtaaaata actatcaatg ctatagttct caaatcagca
     4981 gatatattta aaattgaatc atcaagcaga tttaaaacat atgggcccag aacaatcccg
     5041 acaattaaca tgcctagaag acctggcagt ttaaacttta ttaaaatacc tgaaagcaat
     5101 aaagcgcaaa gaaaaatcaa agctatactt agtaacatac attcctcctt aaacataaaa
     5161 aaactgatgc tctgtttcca gaagtcatca gcttaatgcg gtttttattt aacaggatta
     5221 tatcaaacct gtagggagaa cttcattccc gttagaggtt attctactat taaaattagt
     5281 aagatacaat ctttttttat ttatcttcaa gttttgataa ctcattatca tcaacggcct
     5341 catcatcaga tggtaaaact ttatagcggc tggatctttt aagaacctca acatcctgtg
     5401 cattatatgt acggatatca atttcctcac ctttgtccag tttaacctta agttctcctt
     5461 ttaacattga aacatcgatt acttctccct taccgtcatt ggttttgaca atggcgccga
     5521 taccaggggt tttacgaatt aagtccgtat agacttcatg ctcatacttt aagcagcaca
     5581 taagccttcc gcaaactccg gaaatcttca aaggattcaa agacatagcc tgatcctttg
     5641 ccatttttat tgaaacagtt tgaaaatcat ttaaaaatgt cttacagcaa aacggccttc
     5701 cgcagatacc tataccgcct aaaagtttgg cctcatctct tactccaatc tgtcttagtt
     5761 ctatccttgt tctaaatgta cctgcaagat ctttaaccag ttctctaaaa tccactcttc
     5821 catctgcggt aaagtagaac agtatcttac tcatatcata tgtcgcctct acacctacca
     5881 gcttcatatt aattttatga tcctgtatct tcttttcgca aatagcaaaa gcttcctttt
     5941 ccttattttt tgagtcctca gctttggcaa tatcatcagc tgttgctttt ctaactattt
     6001 cctttaaagg agcaataatt ttggattctt caacctgggc aatttccgaa gctacggttc
     6061 ccatctcgat tcctcttact gtttcaacta ttaccaaatc atctatattc agctcccagt
     6121 ctccaggagc gaaactatat atcttgttcg attgtctgaa ctttatacct acaactgtta
     6181 ccatatattt aattcctcca taagttctat gttcaacgtt cctatagcta tactctgtga
     6241 tacattatct tttatgtatt cagaagcttt catagcagaa ttaaatgcta tcagcaacga
     6301 tttgctgctg tttctctctg ctgttctctt aataatatcc gatatatcgg tatttatcaa
     6361 gtttgatgaa tcattagtcg ctttgtaaac cagcatgtcc cgaaacaagc tttggagtat
     6421 ttctaaaaag accaagacat ctcctttcaa atcttttata tcaaaatcct gctggctttt
     6481 agttatgtaa tctacagctg tctttctgca ctgtataaaa ctttggtcgt ttatgaggtc
     6541 agcagctctt ccaatacagc cttcagcaaa acatgctgca gcatatatgc ttttagtatc
     6601 agcatcagga ttgttctgtt cgagatattc aagaacctcc tgttttgtat ttactccggt
     6661 ataaagtatt atacatcttg aacggattgt atcaattatc tgctcggaat tagttacagt
     6721 cagcataaag aatgcatatg ggggcggctc ttccagtgtt ttcaggaatg cattctgagc
     6781 ctggtctgtc attgtatcag catcatgtat tatatatatc tttttgttgt tagcaaaagg
     6841 catcagttca gtatctgcaa ccagacttct aatatcatca actgatatgg atttttctgt
     6901 ttctagaaca tatttttcct tatatcctgt acagcctttg cacgttttac aagtttccaa
     6961 ccctttttga ttatagcaaa aaagaagttc agcgaaagca tctgctatga gcttctttcc
     7021 tgaacccttt gggccttcaa atatataccc atgacttatc ctgcctttat caatcagttt
     7081 tttcagttga ttctttaaag gctttcttcc tttgatatca tcaaatcctg tcatttcatt
     7141 ctttaatgta tttgactaca tcagttacga atattgtggc tccgcccata gtaatctcta
     7201 taggatatcc agtaagcact gcaccttgtg atgaacctat catggatgag tttactactt
     7261 tttttctgct ctttgatttt gttttgatga tttctatagc atgatccatt ttttcatcat
     7321 ctacgcctat aagaagggtt gtatttccgg attttaagaa tccgcctgtt gaacaaagct
     7381 tggttacacc ataaccttct tggctcaagc ctctcattac gtcaagttcg tcttcatcat
     7441 ttacaattgc gaatataagc ttcatctatt cctccttcat tattatatct acatatttta
     7501 ttatttcttt tgttatttcc tttggtgtct cagttccatc tattcttatt attctttcag
     7561 gaaacctctt aagtaattgc ttgtatcctt catacacttt atagtgaaag gacatatcct
     7621 cattttccag cctgtctgtt tctgtagcat ttttccttct ttttaaagac tgctcaggtg
     7681 ttatatctat aaagaatgtt atctttggaa taatattctt aacagccgca agatttattg
     7741 caagcacagc atcaaaatcc agattccttc cataagattg gtatgcaagg cttgaatcaa
     7801 agaacctgtc cagtattacg acataacctt cattaagcaa tggttgaacc ttttcttcca
     7861 tcatttgtgc tcttgatgaa gcatataaaa gcatttccgt cagagaggcc atttcagtat
     7921 tatccttatt cagtaaaata tgccgtattt cctctcctat ctttgttccg cctggctctc
     7981 taagcatttg agccttgtat cctttgttaa caagatattc actcagtgct ttaatttgag
     8041 tggtctttcc ggaaccatca gttccttcga aagaaattat cattcctttt gccatatatt
     8101 atctatgcct ttccctctat tctgggtttg ttcttatcat aaaccatctt taagccgttt
     8161 aaggtaagca tgctgtccac ataatcgata tattcactct gttttgcaat taaatttaaa
     8221 agcccccctg tagctataac ctttatatcc tcatatgtct ggttatagtc ttcaacaagc
     8281 tcttttttca tatgctttac aatattagct acctgaccga ggtaaccata aacaatacct
     8341 gcctgcatgg aattaatggt attcttccca ataatttttg gaggcattac tatatcaatg
     8401 tttggaagct tagctgcacg tttgtatata gcttcagcag tcagttttac tccagaacat
     8461 ataaccacgc caggacattc aaaatctttt gttacacagc tgaatgtcgt tgcagtacca
     8521 aagtcaataa ctattgcagg ccctttgtat gtctcatatg ctgatacaag attaacaata
     8581 atatccgcac cgagctctct tggattatct atctttatat tcagacctgt cttaatgcct
     8641 tgttctacca gtatcggggt taaattaaaa tatttctcca aactgtgagt aagcgtatac
     8701 attattccag gcaccacaga agatagtatt attgcatcaa catcactaag ctttagtcct
     8761 gctgcattaa acatattcag ataaaaagat ccgaattcat cagcagtcct ctctttattc
     8821 gttgtaagac gccatacatc tatgtatgat ttaccgttat gaacacccat tgctatattt
     8881 gtattaccga catcaatagc taaaagcatc ttatatcctt atcatcttga tttcttctat
     8941 ttcaagacga atctcctttt caattctttg aatatctttt tttacatcat ccactgtttc
     9001 aactatgtca cggccgtttc ttttatcttt tctgtagcgt ttgtaactgt tatagttctt
     9061 actatatgcc ttcttctgag tattaatatc tttatcagca ttatcagtat cttcagggca
     9121 tatgcagtct ttgctttggg cctgttgagt attattgtac ggctttttgt aaggacggtt
     9181 cttgttcttc ttgtagtagt tattattctt tttccttggt tttgactggt tgttgtcact
     9241 atttgaagta acattctggt tttcggtcat tttacgctcc ttgtattttt aacctgatct
     9301 cgtttgagtc aaggtaattt atttctccat cttgttctat aatcagcctt cctgtactat
     9361 caattccctt tgaaagcgct gtatatgata attgctcttt tgtataggtt ataacagtat
     9421 tatccattaa agagtattga ttccacttat ctatgatgca ggctgttttc ccatatttaa
     9481 ttttatcata aagacttagt agatttccac atatattttg gatgagtaaa tcgacaggta
     9541 aattcttttc agtctcaatt atcaggcttg ttgcaatatc tttgatctgt tcattaaaag
     9601 atgactgatt tacgtttatg cctattccaa taatcatata gttaatagaa tcattcacag
     9661 tctggctttc gcataatatc ccacagactt tcctgtaatt aattaaaatg tcgtttggcc
     9721 atttaatgcc acattttatt ttatataaat cttctattga aacagctact gctaaagcag
     9781 ccattattgg aatgagtaaa gactgctttg gagatacttc atttcttttg ggttttataa
     9841 ctaaggaaaa ccatgctcct tcttcattat cggatataaa aaccctgtcc attcttccac
     9901 ggcccgataa ctggattttc gattgaataa ctgtaaactc atcatcaaat aatttagcat
     9961 ttttctttgc ataggtatta gtagagtcaa tgctttctag gctgatgtac ttaataacct
    10021 gcatactatt tacctgtttt tcttactata tactttctgc catttagaag atactcgata
    10081 aatccgtttt tttcgccgat ttttctagca tcagctgaca ctaaaaagta tcctgataat
    10141 gccttttcaa aatatctgct atcagcattt tccaaatctg taagcgaact ttttaaataa
    10201 agttcctcag agtcatctac aatcatcgga atatccatat actttccgag cactttgatt
    10261 ttttcagaaa atactgtagt tgttataagg actataggtt ttctatatgt tcctaatata
    10321 tattcagatg cccttattag tgaatcgcca agtgcttttt gattatccat atccaggtat
    10381 gtgccttcaa ctataaatgc tttttctgct gacacatcaa gactcaaaca agtataattc
    10441 ggcaacttat atcttttaat gtcatcagca ggacaaaggt aggccactat aactgattta
    10501 aagccatttc ctgtttttat ggagttatct ccgtattcag ataacttcat tccaaaagtt
    10561 gtggcatctt taactttgcc atttactaca tgatgataag ttatcataac gaccctgctt
    10621 ctttgtacat actgactata tctgcttcag taagctctcc aggagttgct tttatgctgg
    10681 actgcaacat agccagtgat gcatattttt cacacataga tttatccagt cttggccttt
    10741 ccatatttct taagaaaaag ttacacattg agcctacatc agtaaatccc atctgggcca
    10801 ctatatgaag agttttctgt atttcatgtt ttaacatatg cttgaaatac ggtctgcaga
    10861 aatacagatt agcaaaaccg tgtggcatat tgtgaaaata tgtcatggaa taccctagag
    10921 catgcatcgc tgtagttcct gtatgtgtaa tagccagacc tcccatcata gatgaataca
    10981 ttaggtagta tcgactttct tcatcaaagt ttaattcatc tatagcttta actgcttttt
    11041 caaaagcctt tataccctcc aaagaataca tcatagatat atttgtactt cttagtgaaa
    11101 aataactttc aaacagatga cttgctgcat caaaagctgt tgataccgta tagtcatcag
    11161 gaagactgta tgtgtatttt ggatcctgta atgcatattt tggaaataca aggtctgatg
    11221 cgaaactctt ttttgtctgc cattgatcta cagtaagtat tgaataaggt gtaacttcac
    11281 tgcctgtccc gcttgtggta ggtattgcta ttattggcag agccttttct attttagaag
    11341 ataacaaatc cataccgctc atgttgctta cagctaatac agctatagct tttgctgcat
    11401 caagtgcaga acctccacca attgctatga tatagtcgca gttgaacttt ttactcatca
    11461 ttgctgcatc ttcaacggtg gatatttttg gattttcttc aatcctgtcg tacaatacat
    11521 aaggaccttt ttttaataca tctataacat catcaaaagc cccactcttc tttgcaccgc
    11581 ttctgccagt aacaattaaa ggaaatcttc ccataccttt aaaaacatcc atattgttac
    11641 ggacacagtc aataccaaaa attattctta ccgggttata gcttttaatt tccatctgca
    11701 ggcacatatg agaaggagcc tctgtacatg atataactat ctcctgatgt tatctttata
    11761 ccccttccgt ctcctcttgg tattattaca aaatgatctt tctccaaaga agcaccagga
    11821 gcaaggcttt ttcctgatgt gttatcgctt aatccttcat tgttttcgtt gccgataaca
    11881 acagcgcttc ctgttctcaa tattaattca gaagtaccca gtgcataaat gatgtctccg
    11941 ttatatacat gaagtacctg tattccgtaa ttatctgata tatccctgac tgttatatca
    12001 agaaaatcca ttcgggctct taaggcatct atttctttta gtgcattacc taatgctgtt
    12061 tccagttcag ttattttagc gttatcaacg ggatttacag tagttcctcc tgttgaaccg
    12121 gatgtctgta ttagggcttt gacttccgca attttagcat cgacatattc cacgcttgct
    12181 attaccaaat ctgcagtctg tgctgtagta cagaacatca aactcattat cattaccact
    12241 gccaagaccg ctatagtcgt tttcttatgt tgtctcatct tttgttctcc ttgccttcta
    12301 taggatttat cttattaata cattatacat aacatactga ttcatggtca attatcattc
    12361 atatcaatcc attgcttatc tttaatattt taccctcttt tatgtatact cttggtactc
    12421 tgatacctat tttacaaata tactcataat ttattgtaga acatatgtca gccagatctt
    12481 caacagcaat attaggtccg aaaagttcca cttcgtcatt ttctgaaaca ccttttaaat
    12541 cagttatatc cgccatacac atatccatac atatatttcc gacaacaggc gctgtctgct
    12601 tgtttatgta tacattagct ttttgtgata atactcttga atacccatca gcataaccgc
    12661 aggctattgt tgctataacg ctatctttgt cagtaacaaa tttattccca taactaatag
    12721 gctgaccttt tctcactgtc tttatctgta ttatccttga tttaaatgtc attgcgcatt
    12781 taagtttaag accatcatgt tctttacagt attgtgaagg ataaagaccg tagattatta
    12841 ttccaggtct tatcatgtca agatacttgt caggatgtaa tataaccgct ccactattac
    12901 aaatatgtct taatgctata tcaaaacctt cttcctttat gctttccagc atctctaaaa
    12961 atcttttgta ttgaaggtca gtatatgaca aatcttcttc atccgctgta gaaaaatgcg
    13021 agaatatccc actgtaatga atatttttca gacctagtgc agtcgtcaaa gactctttgt
    13081 catcatagga aaatcctacc ctgtgcatcc cagtatctac ctttatgtgt gcataaagag
    13141 ttttttctaa tttttccgct gtattcgaaa agttttttgc catatcaaga ttggttattg
    13201 tagctgccag attgttttct ataacttgtt caagcctgtc tggacgtata tatcctaaaa
    13261 caagaatagg agctgttatg ccattaattc ttaagttaat tccttcatca agcgtagcaa
    13321 caaccagcat atacacgcca ttttcacata gcgtctgtgc aatctctatt gcgccatggc
    13381 catatccatc ggcttttata acagcagcta tcttaacatc accagctttt tctttaatag
    13441 aagaaatatt atgtgcaata tggtccagat tgatttctgc ccatactctg tcaggctttt
    13501 tcaccagcca cctccttatg tgcgtttggt atatacctaa ttatatctga tgctgtcata
    13561 cttatcatac ctaaatcctt tactgctaaa tctccagcta aaccatggac ataaacagcc
    13621 atacaggtat ttctaaacat atcatctgtc attacagaat atgctgtggt catccctgca
    13681 agcacatcac ctgagccagc ggtagccata ccagcattac ctgtcttatt tatgtatacc
    13741 atatcatttc ctgcaactac agtttcatga ccttttaaaa ctgttataca gccatattcc
    13801 tgataaaaag aatatgcagt tttaactctg tctttttgaa tttcatctat gctcataccc
    13861 ataagaacag acatctcccc aggatgagga agaatggtca ttctgccttt gtatcttttc
    13921 aaaaggtcct tttctaaagc aatcattttt aaagctgtcg catcaacaat aacatgacat
    13981 ttggataatt caagaactct ttttaaaaga agttggtttt gttcatttaa cgaaaggccg
    14041 cagcctatgc aaatgcagtc tttattctga atatgttctt taatatcctc taaaggcact
    14101 gttattcctt caacagtaag ggcgtcgtat tccatcatat gctttttagc agctgtgtaa
    14161 tacactaatc ctgctcctga gcggtagcag gaatccacgc acagtctcaa agctcctgtc
    14221 ataccgatgg aacctgcgat tactaaaaat tttccgtaat ctgatttgtt gctttgagct
    14281 ttccttaatt ggaaaaaagc cgatatgtcc tgtttggaat attcaacaat cttcatgaag
    14341 gtcattatat catcacaaaa gaattaatga aatagaatac tagaatccct gtgtttactt
    14401 gttaatttaa ctttaatatc ctaaaagttt tggagcgaca taacagataa ccgctataac
    14461 cactgcagag attgctgctc ctacgcttac gcttataagg gctttctttt tgttcatctg
    14521 cataaatgct gcaactgcgc ttcctgtcca tactccggta acaggcaatg gtatagcaac
    14581 aaatagagaa aggcctatag tttcgtattt ttcaactttc ttctggcctt tagtaacttt
    14641 tttatctaca aaatcataaa accatttaag aatttttttt gttccaagcc atttgaaaat
    14701 agggctgaag cagaatatta taaagaacat agggatcaaa gaacctacat acgacacaag
    14761 tgttactaaa acgggatcaa gattgtaaac aagtattcca attggaatag ctcctctttg
    14821 ttcaattata ggtatagctg atattcctaa aatatataaa tactgctgta acatttctac
    14881 cacccttttt cagactagac tatatattac tatagttcca ccattttggg aatagtgatt
    14941 tatacttgga tgttccaaaa cgtgaatcta taagtattac aaaacctgta tcggtttcgc
    15001 tccttataac cctgcctgcc gcctgtaaaa ccctgttcat accaggatat gtgtatgaat
    15061 agtcaaatcc gttcatgtct gactcatcaa aatacttctt tataagttct ctttctggac
    15121 ataccattgg caaagcaggc cctataatcg cagcacctga aagcatctca ccttcaaggt
    15181 ctattccttc accgaacaat cctcccatta cagcaaaagc aactaatgta ttatcaccaa
    15241 actgctcgaa cctgtttaga aatattgctc tgtctatatc atccatatat ggcttttgtt
    15301 ccattactac agcatcggga tatattacag taaaaatatc cattaccctt ttcatatatg
    15361 cataagaagg aaaaaagacc atataatttc ctgttttcct ggaaaattct ttttttaaga
    15421 tttccgctat cttctcatat gaatattccc tgtctttgta tttagtggaa atgtctgtaa
    15481 ccagctttat tctcaggttt tcttttggaa aaggagaatc cagtgaaata tagctgtcaa
    15541 catcagcatc tccgcctaaa aggtccatat agtatctacg aggctccagc gttgcactga
    15601 acagaattga tgaacggccc atgtccattc cttcttttaa taaatatgaa ggatcaaggc
    15661 acataagctt tatatcaagt cctttggtat tatctgaata caaggttacg aatctcttat
    15721 catagaaatc tgcaactctg gcaaaataca gaagattgaa cagtccatct aaaaaatctt
    15781 ctcttttatc ttcgggaatc tttttgaaat catacatcat aatactgttt aggcatttgt
    15841 ttacttcttc tacaagatta tcaggatact gctcttgata tatgaagcct tcattcatgt
    15901 tttcaaaatg ctcttttttt aaagaattca tcactgctat aacagcgttc acattttttc
    15961 taagaacttt ccattctttc ttgacgctgt ctttgaaaag tttcatctgt tttaagttaa
    16021 gagtacagga aaacatttcc cttcctcttt ccacaagatt atgagcttca tcaaccataa
    16081 agcagaatgc ctgttcattc tggtaatcaa aataccgctg aagcctgatc ctcggatcaa
    16141 atagataatt atagtcacag attatgaggt cgcagtaatt tgaaagatca aggctcagct
    16201 cgaaaggaca gatgttataa ctgtcagcat accttgctat tatatctttg tcaaaaaact
    16261 gctcatgaag catatcgttc aaacactgcc ttgctcgtag ataatactga tttgctcttg
    16321 gacatttttc agggtcacag tctcttttct ccaaaggaca gactttgtct tttgcgctta
    16381 tacatactgt tcttaagttc agaccgtttt tctcaagcct cttgtaagca tcaacagcaa
    16441 cctttgctgt tacagtttta gcagttagat acataagctt atctacaaga ccagctccta
    16501 aagcctttat agctggaaac attacaccca ttgtctttcc tatacctgta ggcgcagtta
    16561 taaaaagctt tatactgtca tttatgcagt tatatacttc ccccatcagt cttttctgtc
    16621 cattacgata tgaactgaaa ggaaattcca gcctttcaat actttgatct ctaataatct
    16681 gccattttct gattcctgtt atccagttta aatatctgag tactaaatcg gaatatatag
    16741 cttgaatttc gtcatatgta tatgaatatt caaatgtaat gctgtcctga ttctccctgt
    16801 taatgtatac aagtctgact cctatggatt ccaagccatg ttttttcata tacatgcatg
    16861 cataacactg agcctgtcca agatgctccg gtttatcaga aggttttact tcgcttatat
    16921 ccctgtttgt agtctttatc tcgtatatat atgtatttat gccatcttgt gtatcagttt
    16981 ttaacagtcc gtcaatcctg ccggtaatat tcattgagat ttcttcattc tctgccaaat
    17041 catcaatata cacttctttt gtaaaggatt ctttgccatg ctcctttatc atttcttttt
    17101 gaaaacgtat atgattttga gttccttcaa aagcggcctc tttgtaacta aagcctgcat
    17161 gtgtctcaat atccccttta ttgagggaat aatcggcgat atccctaact gatacattaa
    17221 tctttttttg cataaaaaac aaacacctgt ttcttaatta tacactagta agcaggtgtt
    17281 gtatatcaaa tatacctata tattatctac tcttgttccc agttatgata tatgttctgg
    17341 acatcatcaa gatcatctag gttatcgata agtttttcca tgctggcaac tttatcttca
    17401 tcaatcttca catatgtctg cggaatcatc tgcaaatcag cctgtaaaaa attgtatcct
    17461 tttgtctcaa gagcttctct taaagcagaa aactctgcag ggtcacaggt tatttcaaac
    17521 atctcgtcct cgttctcaaa atcttctgct cctgcttcga gcgcttccat cataagctgg
    17581 tctgcgtcta tggttccatc atcttctatt acaattacgc cttttttatc aaacagataa
    17641 gaaacacacc cattagttcc tagatttcca ccaaacttat caaagtagtg acgcacatca
    17701 gatgctgtcc tgtttctgtt atcagtcata gtttccacta ttactgctac cccgttaggg
    17761 ccataacctt catatgttat ctgatcataa ttctcagcgt ttccttctcc ggaagctttt
    17821 ttaatgcttt ttgcaatagt gtcatttgga acattattag attttgcttt tgcaataaca
    17881 tccctaagac gtgaattagc attaggatca ggccctcctg atttaaccgc aaccattatt
    17941 tcgcggccta tctttgtaaa gagttttcct cttgatgaat cgcttctttc tttcttgtgt
    18001 ttaatgttcg cccattttga atgtcctgac atatcaaatc tccaaatata tatttttttc
    18061 tttttctatt tcacatctgc tgttaatcaa ctcgtttatt ttaccaattt cttcctctgc
    18121 tttcaagtcc tccacagcaa acctcacctg aacctgatct tcatacaaga tttcaagtat
    18181 tacattttca ttaagcagcc tcttaatctt ctcatatgaa gagtattctg ttttaataga
    18241 atatatagta cagctaactc ttcttgcaag actagcttta tcaacagcat cctttgcgct
    18301 ctggctgtaa gcccttacaa gcccgcctgt tccaagcaaa gtccccccga aatatcttac
    18361 tactactatt atcacatttt caatattatt ttttctcaat acatcaagaa tcggtaatcc
    18421 tgcagttcct gaaggctctt tatcatcaga atatctttga tatgttactc cgttttcatt
    18481 aattgtatat gcatatgtat ggtgtgtagc atcaggatat ctggatttta tattatccaa
    18541 ttcagctaaa gcctgctttt ccgtgtctgt ttttatggca tatgcaataa aacgggactt
    18601 tttttcaata agttctgatt ctgcattttt tcttattgtg taaattatat tatctgaccc
    18661 cggttgagcc aaagccccca cctctgtcct gtcctatttt tgtaacgtca tcagtttcaa
    18721 caaattccat tcttggaacc tgctgtaaaa ctatctgtgc aatcctgtca ccttttttaa
    18781 tgtgatatat gccctggcgg ttactttttt cgctaatata taaatcacct tcattagctt
    18841 gtgaactgtt gtgaattatt atacctattt cgcctctgta acctgtatct attgttcctg
    18901 gactgtttgt aattctaagt cttgtattta gtgataatcc gcttctagga cgtacctgta
    18961 tttcataacc ttcaggaata gctactttta aacctgtagg tacaacacat gtactgttta
    19021 tatctatatc cctatcttcg catgcgcata tgtccatgcc tgcgtcaaaa ttattggcat
    19081 aagtaggtat ctttgcgtct ggtcttacct tttcaatata tacttttatc atctgaactc
    19141 gttctgcgct tttttgagct gctcttcagt ctctgaaagt gtgtttttaa gctcattatt
    19201 gctctgagtg agcttttcca cttttgcttc taaaatcttc attctggaaa cctgatcttt
    19261 tgattttctt tcaaaatcat ccataaaggc cttctgctta ttagcctggt ctctttcttt
    19321 gaagtaatcg tcagcaatgt taactgctgt tagcactgct gccatagata cactcaattt
    19381 tggattttcc ctcataatgg cagacatttt cttatccacg aataaagcga ttttctctat
    19441 gtactcttca gactcattgc ctttaatcaa atactcatta ttacatattc ttattttcgt
    19501 tgttttcatt ttatgctcca ttgtgttgat gcataaatag tatatattaa gcaacagtat
    19561 tttgcaaaag gtatatatta tt
//
LOCUS       DAOSXM010000035        19109 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2111
            NODE_1815_length_19109_cov_10.323712, whole genome shotgun
            sequence.
ACCESSION   DAOSXM010000035 DAOSXM010000000
VERSION     DAOSXM010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864178
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 19109)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19109)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:24:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,261
            CDSs (total)                      :: 2,229
            Genes (coding)                    :: 2,218
            CDSs (with protein)               :: 2,218
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 3 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19109
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1815_length_19109_cov_10.323712"
                     /isolate="AMR_MDS_2111"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            70..1815
                     /locus_tag="PLQ68_06050"
     CDS             70..1815
                     /locus_tag="PLQ68_06050"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HXK72248.1"
                     /translation="MKHITHKADFCVIGGGIAGICASIAAARHGMSVVLMHDRPVFGG
                     NASSEIRMHICGADRADGKGLRESGIVEEIELENIYHNPQKNYSMWDMVLFSKVKEHK
                     NITCLLNCSCNQAQTDNNRIISIKGWQGTSETFHEVYADIFSDCSGDSILADLVKADY
                     AIGRESSSQYNESIEPKKADLKTMGLSCLIQVKRHNRKIEYIPSEYAEEYKDMFDFAK
                     LMPYRGRGSIEGCTNFWWLELGGVEDSIHDTDKIRDDLIGLSTAVWDFVKNKADYKAE
                     NYSLEWMGFLPGKRESRRYIGDYVLNQNDIQNKTNFHDAVAYGGWSMDDHNPEGFMFE
                     GKPTIYHPAPQPYEIPYRCLYSKNIKNLMFAGRNISASHAAMSSTRVIGTCSILGQAV
                     GLACSIAIKNNTNPRGVYEKHIKQLQHTLMEDDCYIPSLTKPISSLTSQAKYNGKGTL
                     FENLTNGKERNILDEINHWNGEQLILSWDNDIFVKQLRIVFDSELERVEKNMPFMWEE
                     EIYNSQMPKRLVKDFDISVNIDGKWTVTHQVRDNHKRLVKLDINQNIQSIKIENIKTW
                     DGLDKCNIFSFEVNN"
     gene            1830..>2350
                     /locus_tag="PLQ68_06055"
     CDS             1830..>2350
                     /locus_tag="PLQ68_06055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72249.1"
                     /translation="MGFRHFICKNEEYLAEGLIEKIDCGELWIFKGADGSDDFSGFID
                     KLYYEHKDTYGTFCSCIFNNCLRIITAFSNDLPEESADELINLISKARSIHNSFTNIW
                     YKPDNSFLDYLLSNKLEWKSKGHKTYELTYKNGTEYPNYEIPSNIKIIPYEKNYFDRA
                     IVMLDLALAHTFE"
     assembly_gap    2351..2450
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2479..2880)
                     /locus_tag="PLQ68_06060"
     CDS             complement(2479..2880)
                     /locus_tag="PLQ68_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003486808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HXK72250.1"
                     /translation="MKKIIFMILGFLTFGLAAVGCIIPILPTVPFLILSSLCFMQTSE
                     KINNWFQSTKIYKKHFLPYKEQKGMTLKTKLLIIIPVYIILLSLFILKDILAMRIVIA
                     VVLIAKTLVFIKIKTIKQEKKESKNTIQAEQ"
     gene            complement(2934..3575)
                     /locus_tag="PLQ68_06065"
     CDS             complement(2934..3575)
                     /locus_tag="PLQ68_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HXK72251.1"
                     /translation="MKENLTEIVFILDRSGSMAGLESDTIGGFNGLLEKQKQNEGRAI
                     VTTVLFDEKYELLHDRIDIKGIKPINDDDYYVRGCTALLDAVGRTISKIDNVQKNTIE
                     LERAKNVIFVIVTDGFENASKEYTYSIVKKLIEEKKKMGWEFMFLGANIDAAEEASKI
                     GIEKARAATYRADSKGIRTNYQVMHRDICEYRDFGAINSSLQEEIDKADEDNR"
     gene            complement(3658..4737)
                     /locus_tag="PLQ68_06070"
     CDS             complement(3658..4737)
                     /locus_tag="PLQ68_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006830635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HXK72252.1"
                     /translation="MPFKIVLDDITKMNTKAIVNSANEYLIKGGGVCKAIFDAADDIH
                     LEIECASIEHCPVGHAVATKGYQLKAKYIIHACGPVYNKSNVDESEKMLRSAYRSSLE
                     LADKLEIESIAFPLISSGIYGYPRKEAFRIASDEISSFVKTKEMMVYLVLYDKTDYVS
                     DRMRFSRIDQFIKSNYNPQKEIDIRNESYRKWIEKDSSVRYRAEPPKERKRDLNKIKH
                     EVKIENNEEDVPISDTRYARSLDDIFKNMEETFSLMLLRLINEKGKDHTEVYKKANID
                     RRLFSKIKNNKNYIPKKQTVIAFAISLELSIDETKDLLLKAGYALSMSSKFDVIIMYH
                     IKNEIYDIFDINEVLFSYDQLLLGA"
     gene            4933..6444
                     /locus_tag="PLQ68_06075"
     CDS             4933..6444
                     /locus_tag="PLQ68_06075"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HXK72253.1"
                     /translation="MKAIIIGAGLAGLSMAAELSVKGHNVTVYEQNPFIGGIMSHAKK
                     NGYEWEQGPLMLTGFEEDGICYNILRNLDVKYETVLCDRGSVFRDFELWKPKHYQGPK
                     WRKNELLKIFPDESVGLDKYYRFYDRMVTIYDIQDKLEQKNSFLYKILLIFNFLCIKK
                     YASLNAQQLMDLFFKDDKLKTVFTAILADLCIKPSEFSCLGIPSLNIEQAFDKRIPQR
                     KGLLKHQYFSYIVGGTERITEGLGNTIKNRGSKIYTNTEVVKVIVENNKAIGVELKDG
                     SVDKADIVIASAGAKELFNKMVGRDNLTKDFLNILDDILPMEAVFMVHLGLDIDPLQY
                     QKSELCYYYLTYDIDDAVSQMRNGIYHEGEQGFLIYVPSSHGKNMAPEGKHSVTIYTV
                     APDRLNDSDWESKKDYYADKLISLAEKYIPELSKHITEKLILTADDYRKLTHLDRNSF
                     GGLVPHISKKSIPHVTPVNALYFIGAQSQSQGGVSSQIRASHKLAKKIIDDNK"
     gene            6453..7832
                     /locus_tag="PLQ68_06080"
     CDS             6453..7832
                     /locus_tag="PLQ68_06080"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXK72254.1"
                     /translation="MQKESLVVKKSTPLGGKVWFSFILFGLVGQLTWAVENSYFSAYI
                     QQTITAESWATSATIIFSAIFAATATILSGALMDRIGKRRPFVCYGYIVWGLLTMAFA
                     LFGNESYANDAKTITLIVIAFVAMDSIMSFVGSMANDAAYSAWVTDVTDITNRSFVDT
                     IGSMFPLIAMVTILVGFSSFADNGQWALFFGILGAIPAISGIIGLFIFNDSPALKPSP
                     DNTYLNQIAYGFKWKNIKDNKMIYICFLGMMFSSIGMQLWMPYMISLIIDTLQVDYVI
                     SLVTVILASAGISVIAGKIMDKYGKANFYYPVAILGAVGGIIAYLTKFTQGQVAPTTV
                     LLIIGGTFVMASSLMMAGLFNATARDYMPKGRAGCYQGIKMILVIMMPMVLASLIAPI
                     IINSVALAPTAEFILLKPTYANSFLYPHELFLWAGIVTLIVLIPAFFIKKDARNLRKR
                     RLDELTVND"
     gene            8021..8860
                     /locus_tag="PLQ68_06085"
     CDS             8021..8860
                     /locus_tag="PLQ68_06085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72255.1"
                     /translation="MDIKVITDISSINTFMAIDNDVLYVLDNHKLFSYNLLSKNITTS
                     SYRFRQSGLSRRIIFNSKYLYVRQFCTIHEFSKSTLERTRRWKIGKDLSSDITGVCCD
                     EEKVYMGIRNGGFAVLDINSGKIENFNICDSSMWDMTEKDEYIYAGNVDAQLLRIRKS
                     PFKLEDAIKAHKKNCKDVLVHDKFIYTASSDMSIKKFDLDTLTELKERKRAFPRMFSI
                     IGIINNMLICMSMSSKSTAFFDADTLKPLATEPIFCRNMCVYKDKIIFNDNNHIYSKI
                     VTR"
     gene            8946..11768
                     /locus_tag="PLQ68_06090"
     CDS             8946..11768
                     /locus_tag="PLQ68_06090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72256.1"
                     /translation="MAHVCIYSKEEEKAFWDEENRKVILPDKTEHVYVSAYPDTFGKI
                     TSLVEFKKGSYLATDCYYHRLLLIKKDIVKPFGPVLNAPFSVAYTQGYIYVATQPNGI
                     IEPSVITVIDESENILGQMYLDHHVEKNKSYMMYAKDYLNPKLEPEKPFENKSRIESI
                     KSDGKKIEITFKNNENISFDADTPENAMLTNIGWRYVISTSRNIRTKKSIFSYSIDNL
                     SNIDFKNTSIQPSDEPAFQNNIKYTIENNDKKDIYIYGFKNNDMDFSNMPSLLNSILK
                     GCDTDTSKAYAIWDFTKKHMLKGITWPQCSFKNDGKFSAIRFLNSMGSGACGSFNGLF
                     SLLAQSAGIPAKTGSLSNGSHAALRLLLNMKDTYVDALYGGCAPFHGSVIPSEDDDIC
                     TYSELCEDPYLLHRASDESVYELASMIGYKDGWIDGWAEDYSDPYTMGYFLKPKEKME
                     FTYSFNGTYTGPLRPHANILTGKKIINLERNEFVKNFFELNKMKTEGNVFSCEAKADA
                     WYLAQCPYPITGILIEGRLYKGSLNISTDYTDETFEYLKRQEFCAYFGNKKTKSLNNS
                     QNSNYIRFKAFKAQYRIYSITLFFQANRVTLCSLVSGINNLQIEADKGKLRITHEYIQ
                     SSMPRPKAPVLKKKPQGIFEWSADDAVNCEFILSKEPECIIPYAPIFHQVTQKTSVEI
                     HCASILKPDIQYYWKVRSKNRYGIWGPWSAIMKFTYLAPKKPEGITLNIKDRDITLSW
                     IKDKLVSHYQIYSSNELAFIPSESDYTININRTDMTAKKIVCPKNYITETKATSIKLT
                     SDNLIDNLKYCYRVIAVDKLGTKSLSSDFIALPHPWINVESISNYAYSGCAYSSYIKC
                     ISTIGKMRFNRTDDNPMYTDYTDADIITFELEGPSWLKVRSYDGYLYGTADLDDIGIN
                     KFILRAVNRNKQKDEICFTINVMEGV"
     gene            11765..14767
                     /locus_tag="PLQ68_06095"
     CDS             11765..14767
                     /locus_tag="PLQ68_06095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72257.1"
                     /translation="MNRIDFKIEEPFGIYWPNQYTKIKIPCNPDKDINIKSLSLYDSL
                     DNITNADFLSFQNNYIELGLNVSLYPYEIKKMYIEKKDLVKSQRKSKCLSLKRTKSNI
                     EISNGNLKLVIPKNGRYEKNDVPSPILSISSDKLEGVGTFTGHIEKSEVKVSIKNSTL
                     HAKVSIKYNFFEKGIYQIDILTYSKSSELIIHEINNTNNKLSLILSFPSFFAKNTYAR
                     MHTPLKEMGETDEWKRIIYASKLSNEPVLMQPFYTWDINTATLIQYWDDCSLLCIVPI
                     KPGRWENPSQNHILANTINGTGIESKGAKGSKEWVLSLTDAALKEEKITIDLSTKYQN
                     WDYMLNNPIKNVYRADRLCAIYGGPNLTDTLNWMSKIPDNINLTPRLLADEDDIKDTR
                     SRIENWPWMYKTLMNHKDDSTGFDPAGVYMALKDELYAKKAKQCISIWLESRIQLLTC
                     FGYSLHEVVCIRLSRPLRLIAIDYDLTCNSKQYSSQDKRFIYSAFAFLMKSMESRDYW
                     PDRKNGFKMGNRNFHSDRFSALGICACLLNNYEDCPVVIRYVQRQASTELDYCIKESG
                     AWIEAPNYQAYSMNYLITLFAALKNSGYKDFFKNYKFRKTLDFLADIQTCYDMRYGAH
                     MLPTIGDTAANYWSQAFSNIFAWAGSITKSSAFSKRMIRAWNKAGNPVICPGGELNST
                     FKMLLLCNNSLPSAKDKNHSYSEYQGFGSVIKTKKSYLSIKSGDISMHYDHDESSILW
                     YEYNVPVLADIGSQYFPACDASFMHNRVSINMNTDQARGDIIYSSYGKNVSVIVTKTD
                     ISNIQKWPWFPVTDSSWNFRYQSVPYDIPKHKWKRSLIYLNNKASLVVIDEVTGNLPF
                     EQNFLFMTSSYEKDNNKFSFIGQFDVNATAWVFNCTKSNIYDWGYLGLDEPSFKKAFA
                     SDWKKYRWMWEKPIKPMAENVQILRNFCNPNSKVITFITAHKDKVTQNTQLLSNLSTI
                     QWTKNNETITIDLDNVDKVHADELYKEY"
     gene            14768..17230
                     /locus_tag="PLQ68_06100"
     CDS             14768..17230
                     /locus_tag="PLQ68_06100"
                     /inference="COORDINATES: protein motif:HMM:NF036578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72258.1"
                     /translation="MDNKELFLKSAIKYNKSFSDMTLPCNWITDSKISACFCEKGLCA
                     LDYHGNQPVSRNARMIKSGFDNPAFKFTSDIPLSITQYMATSWACEFNLYKKDIKKAR
                     IYSFTYESSIYMHIELYDYDRFSVTFDTDSFYTNVHGNISWDKGKEKNNSIVFKGINK
                     YLLSDWLNEKGAYLIPVKEHRRIFKTQDWIDVNNLDILPLPDEDIIRSNELFIDNACY
                     MAIHCNSHIKISKSGNSNIIELTPKISESYIDLCISFADSFEDSISNADYIIRNSKAI
                     ENMQFSRYSRFLSSPLSIQTDNHSDTEHLISCIPMYIESAKQKDSSMTRASSSSYYWV
                     WGWDNLVTAFEMNKWNDYEGQKNIISFILEHRWIDGSVPHRYDRNYDVMQTMHFCSED
                     ALMITLVYQYCKDSEDLDFLKKIYPDIKQIWNGLLKKADSKGYIIGPGFYPDNLKALG
                     RHENSRTAMETGTFYTAARIMEQLASLLNDNTIEKQAHDIHTNIEKKYIDDFYNTEHG
                     TIADSINEDGSQNNTFPLYAYMGAYTNFGQILFMKKIEQIATFINQNAIHPMGIRVLP
                     LYDKNRDTESVHHSWYPHWDIYAMKLVRLGFMITKDYKRSSAAVNHYLNLTDRMWKNY
                     KAAMELLELDTADYIEGWKQHGQAWNCNCSSGILRTILESAAGVITDFGTISIIPDAC
                     SSVSVKNLWVRKGKWNITHKGNKYFSGITVDGNTYTKTCLVPDEYMTHAEHTLTVSHN
                     SDKDDIKIFSFIGGVIELYEEKENSIAFNAKCNASSDLLVLSPYDFFISINGSTANKE
                     KIANETYRIRLSATAKIEIHIP"
     gene            17318..18922
                     /locus_tag="PLQ68_06105"
     CDS             17318..18922
                     /locus_tag="PLQ68_06105"
                     /inference="COORDINATES: protein motif:HMM:NF015692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HXK72259.1"
                     /translation="MIGKNILELRNKNNLTQEAFAQLLSVSRQAVQKWEKDLSYPDSD
                     KLIKISKIFNVSVDHILGIGSNDPSEEFRVKKEIQPSYSSLHWWELYSSQLNVEYIQS
                     IDEGKDVCKYKNLIDSVSQLETSMQKEQLADIIHTIIQNANTIDDFKFKEPDDLSSIY
                     SLCNPDYIYPFKTINEKVLKKKLLGAWMGRICGCLLGKPIEGIKTDELIPLLKETGNY
                     PMHRYLLRSDMNEQIYDKYKFNLRNRCFADSIDMAPIDDDTNYTVLSNVIIENYTDNF
                     TSEDVAKVWLDKQPKSAYCTAERVAYLNFVKGFLPPQTALYKNPFREWIGAQIRVDYY
                     GYINPGNLKKAAHMAWRDARVSHTKNGIYSSMLIAAIISCAACCDDIQQTIKYALYQI
                     PSTCRLYKAVVGIINQYNNRVSAEDCIKNIHKEYDEHLGHHWCHAISNTCIVIASLLY
                     GNNDFAKSICMAVQAGFDTDCNGATVGSIIGMNKGIEHIPHTWTKPVNNKLNTSIFGM
                     DILSIDELADMTFKHIISFSPQVISK"
BASE COUNT         6859 a         3020 c         3265 g         5865 t
ORIGIN      
        1 taaacaaatg atagtttgta atgaaatgca ttagagtata taattaataa aaataaaaaa
       61 ggggcagcca tgaaacatat aacccataaa gcggattttt gtgtaatagg tggaggaatt
      121 gcaggtattt gtgcaagtat agcagcagca aggcacggaa tgagtgttgt tttaatgcat
      181 gacagaccag tattcggagg caatgcatca tcagagatca gaatgcatat atgcggagct
      241 gacagagcag atggtaaagg attaagagaa agcggtatag ttgaagaaat agaactggaa
      301 aatatatatc ataatcccca gaaaaactac agcatgtggg atatggtttt gttttcaaag
      361 gttaaagagc ataaaaatat tacttgcctt ttaaactgtt catgtaatca agcacaaaca
      421 gataataaca gaataatcag tataaaagga tggcaaggaa catctgaaac ctttcacgaa
      481 gtttatgcag acattttctc agactgttct ggtgacagta tattagctga tttagttaaa
      541 gcagattatg ctataggaag agaatcatca tcacagtata acgagtcaat tgaaccaaaa
      601 aaagcagatc tgaaaacaat gggattaagc tgtcttattc aggtaaaaag acacaataga
      661 aaaattgagt atataccttc agaatatgca gaagaatata aagacatgtt tgattttgcc
      721 aaactaatgc catatagagg aagaggaagc atagaaggct gcacaaattt ctggtggttg
      781 gaactaggag gagttgaaga ttcaatacat gatacagata aaatacgtga tgatcttatc
      841 ggtttgtcaa cagccgtatg ggattttgta aagaataagg ctgattataa agctgagaat
      901 tactctttag aatggatggg ttttcttcct ggaaaaagag aaagccgtag atatataggt
      961 gattatgtac ttaatcagaa cgacatccaa aacaaaacca attttcatga tgcagtggca
     1021 tatgggggct ggagtatgga tgatcacaat cctgaaggat ttatgttcga gggaaagccc
     1081 accatatacc accctgcacc acaaccttat gaaatacctt ataggtgtct ttacagtaaa
     1141 aatattaaaa atcttatgtt tgcaggcaga aacataagcg catctcatgc tgctatgtca
     1201 tcaaccagag taattggaac atgctcaata ttaggtcaag cagtcgggct tgcttgttct
     1261 atagcgatta aaaacaacac aaaccccaga ggagtttatg aaaagcatat aaaacagtta
     1321 cagcatacac ttatggaaga tgattgttat atcccttctc ttactaagcc tatttcttca
     1381 ctaacatcac aggcgaagta taatggaaaa ggaaccttat ttgaaaatct gacaaatgga
     1441 aaagaacgaa atatacttga tgaaattaac cactggaacg gcgaacagct tatattatcc
     1501 tgggataatg acattttcgt aaagcagctt agaattgtat ttgacagtga acttgaaagg
     1561 gttgagaaga atatgccatt tatgtgggaa gaagaaattt ataacagcca gatgccaaag
     1621 agacttgtta aagactttga tatatctgta aatatagacg gtaaatggac tgtaactcat
     1681 caggtacgtg ataatcataa gaggctagta aaactagaca ttaatcagaa catacaaagt
     1741 ataaaaattg agaatataaa aacctgggat ggtctggata aatgtaatat attctcattt
     1801 gaggtaaaca attaattacg aggtactata tgggctttag acactttatt tgcaaaaacg
     1861 aagaatatct tgctgaagga ttgatagaaa aaattgattg tggtgagtta tggattttca
     1921 aaggtgctga tggaagtgat gatttttcag gtttcataga taaattgtat tatgaacaca
     1981 aagatactta tggaacattc tgttcctgta tatttaataa ttgtcttaga ataataacag
     2041 catttagtaa tgatttgcct gaagagtcag cagatgaact aataaatttg atttcaaaag
     2101 caagaagcat ccataactct tttacaaaca tctggtataa accagataac tcttttctag
     2161 attatttact ctcaaataaa cttgaatgga aatcaaaagg acacaaaaca tacgaattaa
     2221 cctataaaaa cggtacagaa tatccgaatt atgaaatacc ttctaatata aaaattatac
     2281 catatgaaaa aaattacttt gacagagcaa ttgtcatgct ggatttagca ttagctcaca
     2341 cttttgaaaa nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn atgtttagat
     2461 aaaattattt gagatatatt actgttctgc ctgaattgta ttttttgact cttttttttc
     2521 ttgttttatc gtcttaatct ttataaacac caaagtcttt gctatcagta caacagctat
     2581 aacaatacgc attgctaaaa tatctttaag tatgaaaaga gatagtaaga ttatatatac
     2641 aggtatgatt attaaaagct ttgtcttcaa tgtcatacct ttttgctctt tgtatggcaa
     2701 aaaatgcttc ttgtaaatct ttgtactttg aaaccagttg tttatttttt ctgaagtctg
     2761 cataaaacat aaagaggaaa gtataagaaa tggaactgta ggaagtattg gtataataca
     2821 tcctacagct gctaatccga atgttaaaaa tcctaaaatc ataaaaatta tcttcttcaa
     2881 atgcaacctc ttctatatta attatatata aaaatatgct taatataaag aacttatctg
     2941 ttatcttcgt cagctttatc tatctcttcc tgtaaagacg agtttattgc tccaaaatct
     3001 ctgtattcac atatatccct atgcatcact tgataattag ttctaattcc ttttgaatct
     3061 gcacgataag ttgctgctct tgctttttct attccaattt ttgaagcttc ttcagcagca
     3121 tcaatattag ctcctaaaaa cataaactcc cagcccatct tctttttctc ttcaattaac
     3181 ttcttaacta ttgaatatgt atattctttg ctcgcattct caaaaccatc agtaacaata
     3241 acgaatatta cattttttgc tctttctaac tctatagtgt ttttttgaac attatcaatt
     3301 ttactaatcg ttcttcccac cgcatctaga agtgccgtac atcctctcac ataatagtca
     3361 tcatcattta taggtttgat tcctttaata tctattctgt catgtaaaag ttcatacttt
     3421 tcatcaaaaa gaactgtagt tacaatagca cgtccttcat tttgtttttg tttttccaaa
     3481 aggccattaa aaccgcctat cgtgtcactt tccaaacctg ccattgaacc gcttctgtct
     3541 agtataaata ctatttctgt taaattttct ttcatttcat ctgcctccaa ttttataatg
     3601 tatctagagt atagatgaat aaaagataca attggtcgct tcaaaggcga caaattctta
     3661 tgcgcctaat aaaagctgat catatgagaa aagaacctca ttaatgtcaa aaatatcata
     3721 tatctcattt tttatatgat acatgataat tacatcaaat ttactgctca tactcaaagc
     3781 ataaccagct tttaaaagaa ggtctttggt ctcatcaatc gatagttcta gtgatattgc
     3841 aaaagcaata accgtttgct ttttgggtat atagttttta ttgtttttga tttttgaaaa
     3901 cagacgtctg tctatgttgg cttttttgta aacttcagtg tgatctttcc ctttttcatt
     3961 tataagcctt aaaagcatta aagaaaaagt ctcctccata tttttaaata tatcatccaa
     4021 actacgagcg tatctagtat ctgaaattgg tacatcttcc tcattattct ctatttttac
     4081 ttcgtgctta attttgttta gatccctttt tctttcttta ggtggttctg ctctgtatct
     4141 aactgaggaa tctttttcaa tccattttct atatgactca tttctaatat caatttcttt
     4201 ttgtggatta tagttgcttt ttataaactg atctattctt gagaaacgca ttctatcgga
     4261 aacataatct gttttatcat acaagactaa ataaaccatc atttcttttg tctttacaaa
     4321 tgaacttatt tcatccgatg ctattctaaa agcttctttt cttggataac catatatacc
     4381 agaggatatc aatgggaatg ctatactttc gatttcaagc ttatctgcta attccagact
     4441 gcttctatat gcacttctaa gcattttttc gctttcatct acatttgatt tgttataaac
     4501 aggcccacat gcatgtatta tgtatttagc ttttaattga tatcctttag tagcgactgc
     4561 atgaccgaca ggacaatgct ctatagaagc acattctatt tctaaatgaa tatcatctgc
     4621 tgcatcaaaa atcgctttac acactccccc gcctttaatc agatattcat tagcggaatt
     4681 aactatagct tttgtattca tctttgtaat atcatccaga acaatcttaa atggcatatc
     4741 tctactccta tacataattg aattaaattt ttacaactga tttctaaata tcttgttgta
     4801 tgtctgaaat gctttctttt cttacattat atcttctttt aggttgattt taaaaggata
     4861 tatatataaa atacatgata tacagaataa ttacttcact agaaactttg atttttagga
     4921 aaggtgaaaa taatgaaagc aataatcatt ggggcaggac ttgcaggtct ttcaatggca
     4981 gcagaacttt cggtaaaagg acataatgtc actgtttatg agcagaatcc ttttatcgga
     5041 ggaataatga gtcatgcaaa aaagaacggt tacgaatggg aacagggacc tctgatgctg
     5101 accggatttg aagaagatgg catatgctat aacattttgc gtaatctgga tgttaagtat
     5161 gaaacagtac tttgcgacag aggttctgta tttagagatt ttgagttatg gaaaccaaaa
     5221 cattatcagg gaccaaaatg gagaaaaaat gaattattga aaattttccc agatgaatca
     5281 gtagggcttg ataaatacta cagattctat gaccggatgg taacaatata tgatatacag
     5341 gataagcttg agcaaaaaaa tagtttcctg tataagattc tattaatctt caactttttg
     5401 tgcattaaaa aatatgccag tttaaatgca cagcagctta tggatttatt ctttaaagat
     5461 gataagctga aaacagtttt cacagctata cttgctgatt tatgcattaa accttcagag
     5521 ttttcctgtc ttggtatacc atctttaaat atagagcagg cttttgataa aaggatacct
     5581 caaagaaaag gtcttctaaa acatcagtat ttttcatata tagtaggcgg aacagaaaga
     5641 atcactgaag gactgggaaa tacaataaaa aatcgcggct caaaaatata tacgaatact
     5701 gaagtagtaa aggtaattgt agaaaataat aaggctatcg gggtagagtt aaaagacggc
     5761 tctgtagata aagctgatat agtaatagca tctgcaggag ccaaagaatt atttaataaa
     5821 atggttggca gagataatct tacaaaagat tttttaaata ttcttgatga catacttcca
     5881 atggaagccg tatttatggt tcacttagga ctggatattg acccgctgca ataccagaaa
     5941 tccgaacttt gctattacta ccttacttac gatatcgatg acgcagtttc tcagatgaga
     6001 aatggcatat atcatgaagg agaacaaggt tttttaatat atgtcccctc ctctcatgga
     6061 aaaaatatgg cacctgaagg aaaacattct gtaactattt atacagtagc tcctgacaga
     6121 ttaaatgact ctgactggga aagcaaaaaa gattattatg ctgataaact gatctctctt
     6181 gctgaaaaat atatacctga attaagtaaa catataacag aaaaacttat tttgacagct
     6241 gatgattata gaaaacttac tcatcttgac agaaacagct ttggtggtct tgtacctcac
     6301 attagtaaaa agagcatacc tcatgtaaca cctgtaaatg ccttatattt cataggtgct
     6361 cagagccagt cacaaggtgg cgtttcatct caaataaggg cttcacacaa attagcaaaa
     6421 aagataattg atgataataa ataggaggga ttatgcaaaa agagagtctt gttgtaaaaa
     6481 aatcaacacc attaggcgga aaagtctggt tttcattcat cttattcggt ctagtcggac
     6541 aactgacctg ggctgttgaa aactcatatt tctctgcata tatacagcag accataactg
     6601 ctgaaagctg ggctacatca gcaactataa tattttcagc tatatttgct gctacagcaa
     6661 ccattctaag tggagcatta atggacagaa tcggtaaaag aagaccattt gtatgttatg
     6721 gatacatagt ttggggtctt ctgactatgg cctttgctct ttttggaaac gaatcatatg
     6781 ctaatgatgc aaaaacaata acccttattg taatcgcatt cgttgcaatg gattctatta
     6841 tgagttttgt gggaagtatg gctaatgatg ccgcatacag tgcatgggtt acagatgtga
     6901 cagatataac aaatcgctct tttgtcgata ccataggctc gatgttccct ctgattgcaa
     6961 tggttacaat attagtcgga ttttccagtt tcgctgataa cggacaatgg gctttattct
     7021 ttggtattct aggtgcgata cctgcaataa gcggtattat aggtttattc atatttaatg
     7081 acagccctgc actaaaaccc tcacctgaca acacctatct taatcagata gcatacggtt
     7141 tcaaatggaa gaatattaaa gataataaga tgatatacat ttgcttttta ggaatgatgt
     7201 tttcaagcat cggcatgcag ctttggatgc cttatatgat tagtcttatt atcgacacct
     7261 tacaggttga ttatgtcata tccttggtaa cagtaatatt agcttcggca ggtatatcag
     7321 ttattgcagg aaaaataatg gacaaatatg gaaaagccaa tttctattat cctgttgcta
     7381 tattaggtgc tgtcggcggt attattgctt atttaacaaa atttactcaa ggacaggtag
     7441 caccgacaac agtcttgctg ataattggag gaacattcgt aatggcaagc tcattaatga
     7501 tggctggatt gtttaatgct acagcaagag attatatgcc aaaaggaaga gcaggatgtt
     7561 atcagggcat aaaaatgatt ttggtaatta tgatgccaat ggtattagca agccttatag
     7621 ctccgataat tataaattct gtggcactag cccctacagc tgagtttatt ttattaaaac
     7681 cgacttatgc taacagcttc ttatatcctc atgaactgtt tttatgggca ggaatagtaa
     7741 cactgattgt tttaatacca gcattcttta ttaaaaaaga tgcaagaaat ctaagaaagc
     7801 gtagacttga tgaattaacg gtgaatgact gatcatacac acgaaaaaaa tcacagattc
     7861 ttatctgttt attgaaaaaa tatagcttat ctgattaata aatcttaata ttcacaagat
     7921 atcataatat attacatata aatattatgt tattttgtgc tcttttacaa ttgaacaact
     7981 tacgatttca tgttataata attaacgtaa ggaggttatt ttggatatta aagtgattac
     8041 agatatcagc agcataaata ccttcatggc aatcgataat gatgttttgt atgtccttga
     8101 taaccataaa ttattttcat acaacctttt atcaaaaaat ataacaacca gttcatatag
     8161 attcagacaa agtggtcttt caagaaggat tatttttaat agcaaatatt tgtatgtacg
     8221 tcagttttgc acaatccatg agttttcaaa atcaactctg gaaagaacaa gaagatggaa
     8281 aatcggaaaa gatctctcat cagatataac aggtgtatgc tgtgatgaag aaaaggtcta
     8341 catgggtatt agaaatggcg gatttgctgt tctagatatt aattctggaa aaattgaaaa
     8401 tttcaatata tgtgattcca gtatgtggga catgacggaa aaagatgaat atatatatgc
     8461 cggaaatgta gatgctcagc ttttaagaat aaggaagtct ccttttaaac tggaagatgc
     8521 tataaaagca cataaaaaaa actgcaaaga tgtgttggtt catgacaaat ttatttatac
     8581 tgcatcttca gacatgtcca ttaaaaaatt cgatttagat acattaactg aactaaaaga
     8641 aagaaaaaga gcctttccaa gaatgttcag cattataggg ataattaaca atatgttgat
     8701 atgcatgtca atgagttcaa aatcaactgc attttttgat gcagatacat tgaagcccct
     8761 agcaaccgaa cccatattct gtcgaaatat gtgcgtatat aaggacaaga taatctttaa
     8821 cgataataat catatttatt cgaaaatagt tacaagataa gtatttataa ataccaaaaa
     8881 gtttttatta aattgatatg ccatatcaat cgtgataaga tttaaacata aaatgaggta
     8941 ccgatatggc acatgtatgc atatattcaa aagaagaaga gaaagcattc tgggatgagg
     9001 aaaatagaaa agttattctt cctgataaaa ctgagcacgt ttatgttagc gcatatccag
     9061 acacttttgg gaaaattaca agcttagttg aatttaaaaa aggctcatat cttgcaactg
     9121 actgctatta tcacagatta ctattaatta aaaaagatat tgtaaaacct tttggaccag
     9181 tactgaatgc tccgttttca gtagcataca ctcaaggata tatatatgta gcaacacaac
     9241 ctaatggtat aattgaacct agcgtcataa cagtaattga tgaatcagaa aatatattag
     9301 gacaaatgta tcttgaccat catgtcgaaa aaaacaagtc atatatgatg tatgcaaaag
     9361 attatcttaa tccaaaactt gaacccgaaa agccatttga aaataaaagc aggattgaga
     9421 gcataaagtc tgatggtaaa aaaattgaaa ttacgttcaa gaacaacgaa aatatatctt
     9481 ttgatgctga tacaccggag aatgctatgt taacaaatat cggctggaga tatgtaatat
     9541 caacaagcag gaatatacgt acaaaaaaat cgatattttc ctattcaatt gataatctat
     9601 caaatattga ttttaaaaat acttcaattc agccaagtga tgaaccagca tttcaaaaca
     9661 atataaagta cacaattgaa aacaatgata aaaaagatat atatatttat ggttttaaaa
     9721 ataatgacat ggacttttca aacatgcctt cccttcttaa cagtatactt aaaggatgtg
     9781 ataccgatac atctaaagca tatgcaatat gggattttac aaaaaaacat atgctaaagg
     9841 gaattacttg gcctcagtgc agtttcaaaa acgatggcaa attttctgct attcgttttc
     9901 ttaactctat gggttcagga gcatgcggta gttttaacgg tctcttttcc ctgcttgctc
     9961 agtctgcagg aatccctgca aaaacaggaa gtttgagtaa tggaagccac gctgctctaa
    10021 ggcttttact taatatgaaa gatacatatg ttgatgctct atatgggggt tgcgctcctt
    10081 ttcacggttc agtgatacca agtgaagatg acgatatatg tacatattca gaattatgtg
    10141 aagacccgta tttattacac cgagcttcag atgaatcagt atatgagctt gcaagcatga
    10201 taggctataa agatggatgg atagacggat gggctgaaga ttattcagac ccatatacta
    10261 tgggttactt cttaaaacca aaagaaaaaa tggaattcac atactctttt aacggtacat
    10321 atacagggcc attgcgtcct catgcaaata tattaactgg taaaaagatc attaatcttg
    10381 aaagaaatga atttgttaaa aacttttttg aacttaataa aatgaaaaca gagggcaatg
    10441 ttttctcatg tgaagcgaaa gcagacgcat ggtatcttgc acaatgccca tatccaataa
    10501 ccggaatatt gatagaagga aggttatata aaggttcatt aaatatatct acagattaca
    10561 ctgatgaaac ttttgaatat ttaaaaagac aggaattctg tgcatatttt ggaaacaaaa
    10621 aaactaaatc gcttaataat tcacaaaaca gcaattacat aagatttaaa gcatttaaag
    10681 cgcaatatag aatttattcc attaccctct ttttccaagc aaacagagta acattatgtt
    10741 ctttagtttc tggaataaac aatcttcaga ttgaagcaga taagggaaaa ctcagaatca
    10801 ctcatgaata cattcagtct tcaatgccaa gaccaaaagc acctgttctg aaaaaaaagc
    10861 ctcagggcat tttcgagtgg tctgcagatg atgctgttaa ttgtgaattt atattaagca
    10921 aagaacctga atgtataata ccatatgctc cgattttcca tcaggtaaca caaaagactt
    10981 cggttgaaat acattgcgca tctattttga agccagatat acaatactat tggaaagtca
    11041 gatctaaaaa cagatacgga atatggggac cgtggagcgc aattatgaaa tttacatatt
    11101 tagctccaaa aaaacctgaa ggaataaccc ttaacataaa agacagggat attacactta
    11161 gctggataaa agataaactg gtatcgcatt atcagattta cagttcaaat gaactggcat
    11221 ttattccatc tgaatcagat tacaccataa atattaacag aactgacatg actgctaaga
    11281 aaattgtttg tccgaaaaat tatataaccg aaaccaaagc tacatcaatt aaacttactt
    11341 ctgataacct gatagacaat cttaaatact gctacagagt tatagcagta gacaaattgg
    11401 gaacgaaaag tttaagttca gattttattg cacttcccca cccttggatt aatgtagaat
    11461 cgataagcaa ttacgcttat tcaggttgtg cttattcatc atatatcaaa tgcatatcca
    11521 caattgggaa aatgcgtttt aaccgtactg atgataatcc tatgtacaca gattacactg
    11581 atgcagatat aattaccttt gagcttgaag gtccttcttg gttaaaagtt cgttcctatg
    11641 acggatatct ttatggaact gcagatttag atgacattgg aataaacaag ttcatcttaa
    11701 gagcagttaa tagaaataaa cagaaagatg aaatatgctt tacaatcaat gttatggagg
    11761 gtgtatgaat cggatagact ttaagataga agaaccattc ggaatatact ggccaaatca
    11821 atacacaaag attaaaatac catgcaatcc ggataaggac ataaatatta aaagtctttc
    11881 actttatgat tcactggaca atattactaa tgctgatttc ttatcttttc aaaacaatta
    11941 tattgaactg gggcttaatg tatctcttta tccatacgaa attaaaaaga tgtatataga
    12001 aaaaaaagat ttagttaagt cacaaagaaa aagtaaatgt ttatctttaa aaagaacaaa
    12061 aagcaatata gaaatatcta atggcaatct aaaacttgtc atacctaaaa acggaagata
    12121 tgaaaaaaat gatgttcctt ctccaatact atctatttct agtgacaaac ttgaaggagt
    12181 tggtactttt actggacaca tagaaaaatc agaagttaaa gtcagtatta aaaattctac
    12241 tcttcatgcg aaagtatcga ttaaatacaa tttttttgaa aaaggtattt atcaaattga
    12301 tattcttaca tattcaaaat ccagtgaact gattatccac gaaataaata atactaataa
    12361 taaacttagc ctcatattat ccttcccttc attttttgca aaaaacactt atgcaaggat
    12421 gcatactcct ttaaaggaaa tgggagaaac agatgaatgg aaaagaataa tatatgcatc
    12481 aaaattatcc aatgaacctg tacttatgca gcctttttac acatgggaca taaatactgc
    12541 tacattaata cagtactggg acgattgttc tttgttatgc atagtgccaa taaagccagg
    12601 tcgatgggaa aatccttcac aaaaccatat tcttgctaat actataaatg gaacaggcat
    12661 tgaaagcaaa ggagcaaaag gaagtaaaga atgggttttg tcattaacag atgcagcatt
    12721 aaaagaagaa aaaattacaa tagatttatc taccaaatac caaaactggg attacatgct
    12781 aaacaatcca ataaaaaatg tatacagagc agaccggctt tgtgcaattt acggcggccc
    12841 taatttaact gatacattga attggatgtc taaaatacct gacaacataa acttgacccc
    12901 aagattatta gcagatgagg atgacattaa ggatacgaga agcagaatag aaaattggcc
    12961 ctggatgtat aaaaccttaa tgaatcacaa agatgattca acaggttttg atcctgcagg
    13021 cgtctatatg gctttgaaag atgaattata tgcaaaaaaa gcaaaacaat gcataagtat
    13081 ctggctagaa tcacgaatac agcttcttac ttgttttgga tattctttac atgaagtggt
    13141 ttgtatccgt ttaagcaggc ctttaaggct tatagctata gactatgacc ttacatgcaa
    13201 ttctaagcaa tactcttccc aagataaacg tttcatatat tcagcattcg cttttttgat
    13261 gaaatcaatg gaatcaagag attactggcc tgacaggaag aatggattca aaatgggaaa
    13321 caggaacttc catagtgaca gattctctgc attaggaata tgtgcgtgtt tattaaataa
    13381 ttatgaagac tgtcctgttg taataagata tgtacaaagg caagcatcta cagagttgga
    13441 ttattgtatt aaagaaagcg gagcctggat tgaagctcca aattatcagg cctattcgat
    13501 gaactattta atcactttgt ttgcagcact taaaaacagc ggatataagg attttttcaa
    13561 aaattacaaa ttcagaaaaa ctttagactt tttggcagat attcagacct gttacgatat
    13621 gcgctatggc gctcacatgc ttccaacaat tggtgatacc gccgcaaact actggtcaca
    13681 ggctttttcc aatatattcg catgggctgg ttcaataacc aaatccagtg cgttttctaa
    13741 aagaatgata agagcatgga acaaagcggg taatcctgta atctgccctg gaggtgagtt
    13801 gaactctact tttaaaatgt tgctgctttg taataattca cttccatcag caaaagataa
    13861 aaatcattca tattcagaat atcaaggttt tggatcagtt ataaagacaa aaaagagcta
    13921 tttaagcatt aaaagcggag atatttccat gcactatgat cacgatgaat caagtatatt
    13981 gtggtatgaa tacaacgtac ctgttttagc agatatcgga agccagtatt ttcccgcttg
    14041 tgatgcctct ttcatgcata acagggtaag cattaatatg aatacagacc aggcaagagg
    14101 tgatatcata tacagttcct atgggaaaaa tgtatctgta atagttacta aaaccgatat
    14161 ttccaatatt caaaaatggc cttggtttcc tgtaactgat agttcatgga acttcaggta
    14221 tcagtctgtt ccatatgata ttccaaaaca caaatggaaa agaagtttaa tttatttgaa
    14281 taacaaagct tctttggtag taattgatga agtaacagga aatcttcctt ttgaacagaa
    14341 ttttctgttt atgacaagtt cttatgaaaa agacaacaat aaattcagtt tcataggcca
    14401 gtttgatgtt aatgctactg catgggtttt caactgcaca aaatctaata tatatgactg
    14461 gggatatctt ggtcttgatg aaccttcatt caaaaaagct tttgcatcag actggaagaa
    14521 atatcgctgg atgtgggaaa aacctattaa accaatggct gaaaatgttc aaatattaag
    14581 aaatttctgt aacccaaact caaaagtcat aacttttata actgctcata aggataaagt
    14641 gacacaaaat acacaactgt tatccaatct ttcaacaata caatggacta aaaacaatga
    14701 aactattaca attgatttgg acaatgtgga taaagttcac gctgatgaac tgtataagga
    14761 atattaaatg gataataaag aattattttt aaaatcagca atcaaataca ataaatcatt
    14821 ttcagatatg acactgccat gtaactggat aacagattca aagatatcgg cttgcttttg
    14881 tgaaaaagga ttgtgcgctt tggactacca cggaaaccag cctgtatcaa gaaatgcaag
    14941 aatgataaaa agcggatttg acaaccctgc ttttaagttt acatcagaca ttccgcttag
    15001 cattacacag tatatggcaa catcatgggc ttgcgagttt aacttatata aaaaagatat
    15061 aaaaaaagcc cgcatatatt cttttactta tgaaagcagt atatatatgc atatagagtt
    15121 atacgactat gatcgatttt ctgttacttt tgatactgat tctttttaca caaatgttca
    15181 tggcaatata tcatgggata aaggaaaaga aaaaaataac agcattgttt ttaaaggcat
    15241 aaacaaatac cttttatccg actggcttaa tgaaaaaggc gcatacctta tcccggtaaa
    15301 ggaacataga aggatattta aaacgcaaga ctggattgat gtcaataatc tggatattct
    15361 tcccttacct gatgaagata ttataagaag caatgaactg tttatagata acgcatgcta
    15421 tatggctatt cattgtaaca gccatataaa gatttccaaa tctggaaact cgaacattat
    15481 tgagctgact cctaaaatta gtgaatcata tatcgacttg tgtattagct ttgcagacag
    15541 ctttgaagac tctattagta atgctgatta tatcattaga aacagcaagg ctattgaaaa
    15601 tatgcagttt tccagatatt caagattttt aagtagtcct ttatcaattc agactgataa
    15661 tcattctgat acagaacatc ttatatcttg tattccaatg tatattgagt cagctaagca
    15721 aaaagacagc tcaatgacaa gagcatcttc ttccagttat tactgggtat ggggctggga
    15781 taatcttgtt actgcatttg agatgaataa atggaatgat tatgaaggtc agaaaaatat
    15841 aatttcattt atacttgaac atagatggat tgacggttca gtaccacaca gatatgacag
    15901 aaattatgat gttatgcaga caatgcactt ctgttctgaa gatgccctaa tgattacgtt
    15961 agtttatcag tactgcaagg attcagagga tttggatttt cttaaaaaaa tatatccaga
    16021 tattaagcag atatggaatg gtctattaaa aaaagcagac tcaaaaggtt atataatcgg
    16081 tcctggcttt tatcctgaca accttaaggc tcttggcaga cacgaaaaca gcagaaccgc
    16141 tatggaaact ggcacattct atacagctgc ccgtataatg gaacaattgg catcattact
    16201 taatgataat acaattgaaa aacaggctca tgatatacat acaaatattg aaaagaaata
    16261 catagatgat ttttataata cagagcatgg aactatagca gatagcataa atgaagatgg
    16321 cagccagaat aatacttttc cgctatatgc atatatgggt gcatacacga attttggtca
    16381 gattttattt atgaagaaaa tagaacagat agcaactttt attaatcaaa atgcaattca
    16441 cccaatgggt atcagggtct tgcctttata tgataaaaac agagatactg aaagtgttca
    16501 ccattcatgg tatcctcact gggatattta tgcgatgaaa ttagtaagac ttggttttat
    16561 gataacaaaa gattataaga gaagctcggc cgctgtaaat cactatttga atttgacaga
    16621 cagaatgtgg aaaaactata aagctgcaat ggaattactg gaactggata cagcggacta
    16681 catcgaggga tggaagcagc acggccaggc ttggaactgt aattgttcaa gcggaatttt
    16741 aagaacgata ttggaatcag cagcgggagt tataacagat tttggaacaa tatctatcat
    16801 acccgatgca tgttcttccg tatcagtaaa aaatctttgg gttagaaaag gtaaatggaa
    16861 tattactcat aaaggaaaca aatacttttc cggaataaca gttgatggca acacatatac
    16921 taaaacatgt ttagtccctg atgaatatat gacacatgca gagcacacac ttacagtcag
    16981 tcataattct gataaagatg acattaagat attttctttt ataggaggag ttatagaatt
    17041 atatgaagag aaagagaata gtatagcatt caatgcaaaa tgcaatgcct cttctgatct
    17101 tcttgtatta tctccatacg attttttcat atcaataaat ggctcaaccg caaataaaga
    17161 gaaaatagca aatgaaacat accgaataag gttatcagct acagcaaaaa tagaaattca
    17221 tataccttaa tgcaacttca agttgcttaa ttgtatacat atgtatgttg atatttataa
    17281 ccctcaagta taagatttac ttaatggagg aacagtcatg atcggtaaaa acatacttga
    17341 attaagaaat aaaaacaatc tcacacaaga agcatttgct caattgctat cagtaagcag
    17401 acaagctgta cagaaatggg aaaaggattt gtcatacccc gatagcgata aactcataaa
    17461 aatcagtaaa atattcaatg tatcagtcga tcacattctt ggaataggca gtaatgaccc
    17521 ttctgaggaa tttcgtgtaa aaaaagaaat tcagccaagc tattcctccc ttcactggtg
    17581 ggaattgtat tcttcccagt taaatgttga atacattcaa tcaatagatg aaggaaaaga
    17641 tgtatgtaaa tataaaaacc ttatagatag cgtcagtcag ctggaaacca gtatgcaaaa
    17701 ggaacagctt gcagatataa ttcataccat aatacagaat gcaaatacaa tagatgattt
    17761 taagttcaaa gaaccagatg atttatcatc aatttattct ctttgcaatc cagattatat
    17821 atacccattt aagaccataa atgaaaaagt gttaaaaaag aaactacttg gagcgtggat
    17881 gggaagaatt tgcggatgtc ttttaggtaa accgattgaa ggaataaaga cagatgaact
    17941 gatacctctt ttaaaggaaa caggtaatta tcctatgcac agatatcttt taagatcaga
    18001 tatgaatgag cagatatatg ataagtacaa attcaatttg agaaaccgct gttttgccga
    18061 tagtatagat atggctccaa tagatgatga taccaactat acagttcttt cgaacgttat
    18121 aattgaaaac tatacagata acttcacttc agaggatgtt gccaaagtat ggctggataa
    18181 acaaccaaaa agtgcgtatt gcactgctga aagagtagca tacttaaatt ttgtaaaagg
    18241 ttttctgcca ccgcagacag ctttatacaa aaatcctttt agagaatgga ttggggcaca
    18301 gataagggtt gactattatg gatatatcaa cccgggtaac ctgaaaaaag cggcacatat
    18361 ggcatggagg gatgcaagag tatcacacac aaaaaacggt atttattcct ccatgcttat
    18421 agctgcaatc ataagttgtg cagcctgctg tgatgatata cagcagacaa taaaatatgc
    18481 actctatcag ataccttcaa cttgcagatt atacaaagca gtagttggaa taataaatca
    18541 gtataataac agagtttcag ctgaagactg cataaaaaac atccacaaag aatatgatga
    18601 gcatttaggt catcactggt gtcatgctat atccaataca tgtattgtaa ttgcatcatt
    18661 actgtatggt aacaatgatt ttgcaaaatc aatctgcatg gcagtacaag caggatttga
    18721 cacagactgt aatggagcta ccgttgggtc aatcataggg atgaataaag gaattgaaca
    18781 tataccgcat acatggacaa aacctgtcaa caacaaatta aatacctcga tattcgggat
    18841 ggacatatta tcaattgatg aacttgcaga tatgactttt aaacatatca tcagtttttc
    18901 accacaggtt ataagcaaat agtgcaaata acaagataat accaagacca aatataaaaa
    18961 tgtatgatga attaaaaaat gtatatataa tattatattt tttactattg cattgttcta
    19021 tttcatggta aaattattct aaaggatata tttaacctgt ctttggcctg aagcattaaa
    19081 aaatggtgag agttacagta aaactgtac
//
LOCUS       DAOSXM010000036        18897 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2111
            NODE_1847_length_18897_cov_9.772423, whole genome shotgun sequence.
ACCESSION   DAOSXM010000036 DAOSXM010000000
VERSION     DAOSXM010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864178
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 18897)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18897)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:24:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,261
            CDSs (total)                      :: 2,229
            Genes (coding)                    :: 2,218
            CDSs (with protein)               :: 2,218
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 3 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18897
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1847_length_18897_cov_9.772423"
                     /isolate="AMR_MDS_2111"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1136)
                     /locus_tag="PLQ68_06110"
     CDS             complement(<1..1136)
                     /locus_tag="PLQ68_06110"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HXK72260.1"
                     /translation="MRYIKDIQTKYSYELVVCGGGMSGFASAISAASKGIKTLIVERN
                     GCLGGVATSCMVNHLLGAKHVDSSTESLKKNISGLFDVLADRLISKNYAIDPNKTALY
                     DNPHGWYSTLAEGLVVSSEGLKMEMELMCKESGVDILYLSHICDVIKDESSISHIIVH
                     NKSGLFAIEGKCFVDATSDADIAVYCGCPYIKGRETDGLMAPASLMMHLENVNTEKLR
                     TYIRENNEHRFKKLIKVLKEKGIWSFPYDIFICVQLTKPDTYMINTIRQVGVDGTDGL
                     SISNAILDGRDESFRLFEIVKNHFPGFENSAIFRIADNIGIRETNRIKGVYTLTVEDL
                     IENKKFDDTIAISSYGWDLPDPNKPSIQPMEKTKRKNCYTYIPY"
     gene            1228..2310
                     /locus_tag="PLQ68_06115"
     CDS             1228..2310
                     /locus_tag="PLQ68_06115"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF013345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HXK72261.1"
                     /translation="MRTPVTCIDKTILEKNINQMQSLADSKNKLLMPMMKTHKSTQIA
                     KMQLEAGCYGFLCGTIDECIAAYTIGAKNIMYAYPISNIENLQRLCKISKETNIMLRT
                     DSYECAMLAKTAAEQENLIFQMSVIVDSGLHRFGLEPSKAANLAYQISSFSNIEFIGF
                     STHPGHVYKKTYEYVEQIAKEECESLVLAKKELLSLGVKTKFLTTGSTPTVRMAVNCK
                     DIDIIHPGNYVFNDFIQISLKAATEEDCALTVSASIVSNPEEGIFIMDAGAKCLGLDK
                     GAHGNSAILGYGYILDHPKACVVSLSEEVGIISANQEQFSIGDIICIIPNHSCSTANL
                     TNRLYCIEEDEYIDVDIRGNSYNFYL"
     gene            complement(2321..3307)
                     /locus_tag="PLQ68_06120"
     CDS             complement(2321..3307)
                     /locus_tag="PLQ68_06120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72262.1"
                     /translation="MKKILILLFTVILIASITGCKPSEDRQYDKYQEFYTTAEKEYEI
                     PGLNDKFIPQGLCYIEKANIIAISGYMDDNSASRIYLIDAKTGSSIKKVLIKNSDGTD
                     YTGHGGGIVSYEDYLWISSSSYAHRISLLDLLNQNDEGYINIIDSFDTNTNASFIYCK
                     NNQLWVGEFYESKNYSTDESHHFLTADNKKQNALICIFDIDLSKPYGISSTVPLKAIS
                     ITDKVQGICITETDKIVLSISYGRKNDSYLYIYENIINKSDDAKISIAGNDVGVLFLD
                     SDHLVKVIKAPPMTEGLMDYADKVYILFESAASKYIETSYYPQKNMISIKID"
     gene            3843..5504
                     /gene="ilvB"
                     /locus_tag="PLQ68_06125"
     CDS             3843..5504
                     /gene="ilvB"
                     /locus_tag="PLQ68_06125"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009429945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HXK72263.1"
                     /translation="MKEIGTKIIIDTLIEQGCDTVFGYPGGAVLNIYDELYKNSDKIK
                     HVISAHEQGAAHAADAYSRVTGKAGVVISTSGPGATNLVTGIANAYLDSSPIVAITGN
                     VALPLLGKDSFQEVDITGITMPITKHNYIVKDINELADVIREAFMIAQSGRPGPVLID
                     IPKDIQTAMCEYEPKKPQRKGIKYEVTEKQLDQVAKVIAESERPYIYCGGGVVISQAG
                     EEIAKLAQKIDSPIGFSMMGLSAVSADNPYCLGMTGMHGRYASTKANSEADLIIAAGV
                     RFSDRATGNKSRYTQKSKVIHIDIDPAEIDKNISAYFGLIGDVKTVLSKLLSKVKQQK
                     HTQWMNQIEIMREEEAAQFVKDAVMHPKDIIEKVMEYTQDDTVIATDVGQHQMWVAQY
                     YRFKKPRTYLTSGGLGTMGFGMGAAIGGCFASGCKRTILFTGDGSFGMNLNEMATAVS
                     NNLPLVIIIFNNGVLGMVRQWQNTFYDQRYSQTTLNRKTDFVKLAEAFGASGYKAMDM
                     DMFEDALKKAFATDGPVVIDCSINKDEKVLPMIPPGGSIDNIIIR"
     gene            5519..6034
                     /gene="ilvN"
                     /locus_tag="PLQ68_06130"
     CDS             5519..6034
                     /gene="ilvN"
                     /locus_tag="PLQ68_06130"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014967365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HXK72264.1"
                     /translation="MKKEQFILSVLVANHAGVLTRISGLFSRRNYNIDSLNVAETEDA
                     KLSRMTIIARGDEATRQQIAKQLSKLHDVKKIDILPPENTVLREHLLLKIKVTKKTRG
                     EIVESVNIFRGRVVDFNFDSLTVEVTGETDKLDAFIRFVSQFGILEMCRAGALAVKRG
                     VLMSAEQTDEY"
     gene            6049..7038
                     /gene="ilvC"
                     /locus_tag="PLQ68_06135"
     CDS             6049..7038
                     /gene="ilvC"
                     /locus_tag="PLQ68_06135"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014355780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HXK72265.1"
                     /translation="MAVMYYEKDCDMSLLKNKTVAVIGYGSQGHAHALNLKDSGVNVI
                     VGLYEGSKSMEKARQAGLKAMVTAEAVKQADLTMILVNDELQAAIYKKDIKDNLKPGS
                     TLAFAHGFNIHFNQIIPRDDINVIMIAPKGPGHTVRSQYEENKGVPCLIAVYQDYTKN
                     AKQIALAYANGIGGARAGVLETTFKEETETDLFGEQAVLCGGVTALMKAGFDTLVEAG
                     YQPESAYFECIHEMKLIIDLVVSQGISFMRYSISDTAEYGDYVSGKRLITDDVKKEMK
                     KVLTEIQDGTFATNWILENQANRPSFLAMRRMQNEHLSEKVGAELREKMSWKK"
     gene            7057..8721
                     /gene="ilvD"
                     /locus_tag="PLQ68_06140"
     CDS             7057..8721
                     /gene="ilvD"
                     /locus_tag="PLQ68_06140"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015536078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HXK72266.1"
                     /translation="MNSDKIKKGAEACPKRSLLKSMGYTDSQIEKPLIGIVNSFNEIV
                     PGHIHLKNIARAVKDGVLAAQGTPIEFNVIGVCDGLAMGHVGMKYSLSSREVIADSIE
                     CMAMAHAFDALVFIPNCDKIVPAMLMAAARINVPSVFVSGGPMLSLNDGKNDLDLNSA
                     FEAVGAFMNNTIDEKELLRIENNSCPSCGSCSGMFTANSMNCLSEALGMAQWGNGTIP
                     AVYSKRIQLAKKAGEAIMSTLKENILPRDIMTKKAFENALTVDMALGCSTNSVLHLMA
                     IAHEAQVDLDLEMVNRISEKTPNLCKLAPAGPYHMQDLDAAGGVHRVMYELCKKSLIN
                     EDAMTYSSKTMKEMIDNHISSISRNDVIRPIDNPYSDKGGIAVLFGSLAPRGSVVKRS
                     AVAQQMMKHTGPARVFNSEEEACSAIYSGSINKGDVVVIRYEGPAGGPGMREMLNPTS
                     AIAGMGLDKHVALITDGRFSGATRGAAIGHVSPEAASEGPIAYVHENDMISIDIPNNR
                     LDLEVSSSELEQRIKTTTILKKENLKGYIARYARNVSSADKGAVVS"
     gene            8733..>9754
                     /locus_tag="PLQ68_06145"
     CDS             8733..>9754
                     /locus_tag="PLQ68_06145"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013657835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HXK72267.1"
                     /translation="MERQIRIFDTTLRDGEQAPSCSMNTEEKIEIAKQLEILGVDCIE
                     AGFPISSEGAFQSVERIAKTVKNSQITALCRAVEKDIDAAWNSIKGAQSPMIHTFLAT
                     SAIHMEHKLKMSPEKVLENIDYAVRYAKKYCKDIEFSAEDAMRSDKDFLVKAFNVAIK
                     AGATVINVPDTVGYMLPEEIYDRIRYLFDNVNGIEDVIVSVHCHNDLGMAVANSLAGV
                     KAGASQVECTINGLGERAGNAALEEIVMALKTRRPLFNAYTNIDTKQISRSSKLVYNI
                     IGQTAPINKPIVGANAFAHESGIHQHGVLSARETYEIMKAEDLGIVENKLIIGKHSGK
                     HAIADRL"
     assembly_gap    9755..9854
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <9855..10091
                     /locus_tag="PLQ68_06150"
     CDS             <9855..10091
                     /locus_tag="PLQ68_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006875367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-isopropylmalate synthase regulatory
                     domain-containing protein"
                     /protein_id="HXK72268.1"
                     /translation="GPINAAYNSINSLTNNICERLVNYSIHSVSDGNDALGEVTVKLK
                     SKDLIVTGRGLSTNIIESSIMAYINGINKILEME"
     gene            10108..11676
                     /gene="cimA"
                     /locus_tag="PLQ68_06155"
     CDS             10108..11676
                     /gene="cimA"
                     /locus_tag="PLQ68_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013484851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043714 - (R)-citramalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HXK72269.1"
                     /translation="MSKRKIEIFDSTLRDGAQGEGISFSVSDKLNIVKALDSFGVDYI
                     EAGNPGSNPKDMEFFEKASNLKLNNAKLCAFGSTARNGIHPKEDKNVKALLSAGTETI
                     VIFGKSWDLHVEKVLNISLEDNLKIVEDTISYLKSEGKYVIFDAEHFFDGYKSDTGYS
                     MKVLEYAIKGGADVLALCDTNGGCLPNDVESITKEVIKRFPGIKISIHCHNDTGCAVA
                     NSIIAVGAGASQVQGTFIGVGERCGNADLSVIIPNLKLKMGYDCNGDISQLYATSRRI
                     ADISNTQIDNNRPYIGKSAFAHKGGMHIDGVLKINSSFEHVNPESVGNKRKLLMSEVA
                     GRSTLIAKASSIVPNLSKDSKEVALILEKLKEMEHYGYHFEAADASFELLVKRTMGLY
                     KPHFNLVLYKTMGEFPVPVGGMPASATIKVEVDGKEEITAVMGNGPVNALDLALRKSL
                     TVFYPAIKDIRLTDYKVRVLEENSTTAAKVRVLIETADEKRSWSTIGVSNDIIEASLI
                     ALVDSIEYKLAMNE"
     gene            11690..12967
                     /gene="leuC"
                     /locus_tag="PLQ68_06160"
     CDS             11690..12967
                     /gene="leuC"
                     /locus_tag="PLQ68_06160"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013483691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HXK72270.1"
                     /translation="MTSMTMTQKILATHAGMNEVTAGQLILANVDMVLGNDITSPVAI
                     NEFGKIKTDKVFDKEKIALVMDHFAPNKDIQAAQQCKLCREFSNEKEITNFYDTGDMG
                     IEHALLPEKGLVTSGDIVVGADSHTCTYGALGAFSTGVGSTDMACAMATGKAWFKVPS
                     AICFNLTGKLNEYVSGKDVILHIIGMIGVSGALYKSMEFKGSGLSNLTIDDRLCMANM
                     AIEAGAKNGIFEVDNITLEYLSSVGAKKPVIFSADKNAVYDKTYDIDLSSIKSTVAFP
                     HLPENARTFDNMEDIIIDQVVIGSCTNGRLSDLIAAAEIIKGKKVAKNVRTIIIPATQ
                     KIYLQAMEMGLLKIFIEANCAVSTPTCGPCLGGHMGILAKGERAVATTNRNFVGRMGH
                     PESEVYLASPQVAAASAIAGKIAHPYDVMKKGE"
     gene            12970..13467
                     /gene="leuD"
                     /locus_tag="PLQ68_06165"
     CDS             12970..13467
                     /gene="leuD"
                     /locus_tag="PLQ68_06165"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HXK72271.1"
                     /translation="MSVRGKVIKFGNNVDTDVIIPARYLNTTDKLTLASHCMEDIDKT
                     FVNRVNKGDIMVAGNNFGCGSSREHAPIAIKESGISLVIAKDFARIFYRNSINIGLPI
                     IESVEASEQINEGDEIEADFEKGVIINHTQNKEYVIEPFPDFIRKIIQAGGLIEAVNK
                     GMLAD"
     gene            13500..14549
                     /gene="leuB"
                     /locus_tag="PLQ68_06170"
     CDS             13500..14549
                     /gene="leuB"
                     /locus_tag="PLQ68_06170"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009986579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HXK72272.1"
                     /translation="MKLKIGLVKGDGIGPEIVECAIKVLDKVLDKTEYSAVYEELCAG
                     GVAIDKYGVPLPDETISKALECDSVLLGAVGGPKWDTLPGHLRPERALLGLRKELGLF
                     ANIRPVKLYDALKDRCLLKDSDEIDIVIVRELTGGIYFGKRGRNQTTDGVEAFDTETY
                     NEKEIERIGRVAFEYAMKRRKKVTSIDKANVLESSRLWREVMHNLKKQYPEVEYADML
                     VDNAAMQLIRNPKQFDVMVASNMFGDILSDEASQLTGSIGMLPSASLGNTKKGLYEPI
                     HGSAPDIAGKNLANPIATVLSVSMMLSYSFNLDNLAKKIDNAVEKTLKNGFFTKDIGG
                     DLTTNQITDKILEYL"
     gene            complement(14544..>16471)
                     /locus_tag="PLQ68_06175"
     CDS             complement(14544..>16471)
                     /locus_tag="PLQ68_06175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72273.1"
                     /translation="KALKHSGYKPKISKNPEDAINNADYDSSIIFLADGYPTATFELS
                     KSIIDKIRSKKIKAYVEYPLSINDIKTEEPKTIKYERAIIQNDFFSSNPAKNSICYIN
                     GCWYRYSDMITDTSISLAKAAGYDSLAYDMPKESIPVLGYLNGNEDILICTTSLSDFI
                     KGRYSPYLRWIGIWEGILKHLGLNIELSWQSDIEFMHESDAHITKKGYISAFKKNCIW
                     SYNNILTKMDDSYFAIEGYQSDITYDGRQFLRLSNRGDCNLETAMQFGLYASYTNNPE
                     YEKIAKNMATNIFTLPSFANTDPSSSMYGLTNWYANGKAFYGDDNARVMLSSLILRNT
                     LGFKDLDEYILKCVFGNLRTTGKLGFRRMRLDDGRFKDMIWKDYYNEDFIHLAPHYQS
                     YLWAVYLWVYKMTGIEELYIKALKAIEITMDAFPNELIWTNSLTAEIARMILPLCILY
                     EVTNKEEHKKWLEKAVYGILEYQQPCGAIRDGFKDVSKGRYPPPKTNEDYGTTEASLI
                     QNNSDPATDLMYTANWAFIGLHEASLILEDKKINTACTLLADFLTKIQIKSTKQPYLD
                     GAWMRGFDYSKWEYYSSAADIGWGAACVESGWSNAWIASILYLRANKKPVFTGSFENE
                     LSDKAKRIYKEMITDQL"
     assembly_gap    16472..16571
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<16572..17327)
                     /locus_tag="PLQ68_06180"
     CDS             complement(<16572..17327)
                     /locus_tag="PLQ68_06180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HXK72274.1"
                     /translation="MKEEVIKMFDYSVVRNNERYPWVVFIHGFGGSNITWKGQIEAYS
                     KYFNLLLLNLHSNSAKLFKLDIQSISSNIYESLCYENIKKAFFVSMSSGSLVALAYAS
                     CYPDSVLGMIMAGGMIHFNVLTKTLLYLARKLKNVIPYLTLYKFFAHIIMPGKNHMKS
                     REIFVRESDKLGHVEFAKWVDILPVLIDNRQILNKININNPPIPFIYIMGKQDHLFKN
                     PIQKDISLLKNAKVHIINNCGHVCTIEKTDEFNQ"
     gene            complement(17299..18495)
                     /locus_tag="PLQ68_06185"
     CDS             complement(17299..18495)
                     /locus_tag="PLQ68_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011498258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HXK72275.1"
                     /translation="MAKSILDHMTMNRFITIKELINGWRYKTFIPRVLLIDPTSACNL
                     KCKGCWASDYSRTDHLSYEKLDDIIAQARKLGIGDVLMTGGEPLMRKDDIIKLCDKHR
                     RTTFAAFTNATMIDEKFADEMVRLGNLNMFLSIEGTREETDFRRGQGVYDKVIKAMDI
                     LKSRDIGFAFSACYHSKNYKTIASDEFLDYMREKGCWFGWLFNYVPVGSDADLELTCN
                     AEQRAYVQQKISDYCIKNEYTIVDFWNNGHLAFGCLAAGNGFIHINSKGDVEPCAFCH
                     YSDSNIHEVSLLDALRSPFFTAFRKAQPFSSNPLRACPLVDVPEGIVNVVKEGNAHST
                     HFNNPESAETLAQKNYKRAEQWKIKAEELFKNMPVHNQKNFPIFLKYHRFKKRTSDER
                     RSNKNV"
     gene            complement(18585..>18897)
                     /locus_tag="PLQ68_06190"
     CDS             complement(18585..>18897)
                     /locus_tag="PLQ68_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015052242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72276.1"
                     /translation="DLKDEIPSELSALLPSSVNIYSSGTVSIQNNQLTGNPDVFKIGP
                     VNLPDEYMTEESIEVMEEVFSRIYTIIPGLEIYSLGSDANGNFVFDGLIPQTVNAIPK
                     Y"
BASE COUNT         6288 a         2926 c         3615 g         5868 t
ORIGIN      
        1 ctgtagggta tgtatgtata gcagtttttt ctttttgtct tctccatagg ttgaatagaa
       61 ggtttattgg ggtcaggaag gtcccatcca taggaggaaa tagctattgt gtcatcaaat
      121 tttttatttt caatcagatc ttctacagtt agcgtatata ctccttttat tcggttagtt
      181 tcccttatac caatgttatc agctattctg aaaattgctg aattttcaaa accagggaag
      241 tgatttttaa ctatttcaaa caatctgaag ctttcatctc ttccgtctaa gattgcattg
      301 cttattgata aaccatctgt accatcaaca ccaacctgtc ttattgtatt gatcatgtat
      361 gtgtcaggtt tggtaagctg tacgcatata aatatgtcat atggaaaact ccaaatacct
      421 ttctctttga gaacttttat taattttttg aatcgatgct cattattttc tcttatatat
      481 gttcttaatt tctctgtatt tacattttcc aaatgcatca ttaaagaagc tggagccatt
      541 agcccatctg tttctcttcc ttttatataa ggacatccgc agtaaactgc aatatcagca
      601 tctgatgtcg catcaacaaa gcattttcct tcaattgcaa aaagaccact tttattatga
      661 actataatat gtgaaattga tgattcatct ttaataacat cacagatatg ggataaatat
      721 aatatatcca cacctgattc tttgcacata agttccatct ccatcttcaa accttctgaa
      781 gatactacca aaccttcagc taaagttgaa taccagccat gaggattatc gtataaagct
      841 gttttatttg gatctattgc ataatttttt gatatgagtc tatcagcaag aacatcaaac
      901 aatcctgaga tgttcttttt taaagattcc gttgaactgt ccacatgctt agctccaagc
      961 aaatgattta ccatacagct ggtagcaaca ccacctaagc atccgtttct ttccacaatc
     1021 aaagttttga tacctttaga ggcagcactg attgcagatg cgaatccact cattccacct
     1081 ccgcaaacta ctagttcata tgagtattta gtttgtatat cttttatgta tctcataaca
     1141 ccccttaatt tgaatcttga tgaacttata taaaattata tcatatgaat taatgtgtgt
     1201 gttaaaatca actaaagggg aaataagatg agaacaccag ttacttgtat agataagacc
     1261 atacttgaaa aaaatataaa tcagatgcaa tcattagcag atagtaagaa caagctgctt
     1321 atgcccatga tgaagactca taaaagcacg cagattgcaa aaatgcagct tgaagcaggc
     1381 tgttatggtt ttctatgcgg aactattgat gaatgtattg cagcttacac tataggagca
     1441 aaaaatataa tgtatgcata tccaataagt aatattgaaa atttacaaag gctatgcaag
     1501 attagtaaag aaactaatat aatgctaaga actgattcat acgaatgcgc aatgcttgct
     1561 aaaactgcag ctgaacaaga gaatttgatt tttcaaatga gcgtgatagt agatagcgga
     1621 ttgcacagat ttggacttga accgtcaaaa gcagcaaatc ttgcatatca gatttctagt
     1681 ttttccaata ttgagtttat agggttttct actcatccag ggcatgttta taaaaaaaca
     1741 tatgaatatg ttgaacagat agctaaggaa gaatgtgagt ctctcgtttt agcgaaaaaa
     1801 gagcttctga gtttgggtgt aaaaactaaa tttcttacaa ctggaagtac acctactgtc
     1861 cgaatggctg ttaattgtaa ggatatagat attattcatc ctggtaatta tgtattcaat
     1921 gatttcattc aaatatcttt aaaagctgca acagaagaag actgtgcatt gacagtatca
     1981 gcttccatag tttctaatcc tgaagagggt atttttatta tggatgctgg tgctaaatgt
     2041 ttaggcttgg ataaaggagc tcacggtaat tcagcaattt tgggatatgg gtatatttta
     2101 gatcatccaa aagcatgcgt ggtttcctta agtgaagaag taggtattat atctgctaat
     2161 caagaacagt tttctatagg tgatataata tgcataatac ctaatcattc ctgttctaca
     2221 gctaacctga caaacagatt gtattgtatt gaggaagatg agtacatcga tgttgatata
     2281 agaggtaata gttataattt ttatctctaa tctttaatct ttaatctatt tttatagata
     2341 tcatattctt ttgaggataa taggaagttt caatatattt tgaagcagct gattcaaaaa
     2401 gaatatatac tttatccgca taatccatca gaccttccgt cataggaggg gcttttataa
     2461 cttttacaag atggtcagaa tctaaaaaca atacaccaac atcatttcct gctatggaaa
     2521 ttttagcatc atcgctctta tttattatat tttcatatat gtacaaatat gagtcatttt
     2581 ttcttccata tgatatggat aatacaattt tatctgtttc tgttatgcat atgccttgta
     2641 ctttatctgt tatagaaata gcttttaatg gaacagttga agatattccg taaggttttg
     2701 aaaggtcaat atcgaaaatg caaattaaag cattttgctt cttattatca gcagtcaaaa
     2761 aatgatgaga ctcatctgta gaatagtttt tgctttcata aaattcgcct acccatagct
     2821 ggttgttttt acagtaaata aatgaggcat ttgtattagt atcaaaactg tcaattatat
     2881 taatataacc ttcatcattt tgatttaata aatctaaaag agatattctg tgagcataag
     2941 aagatgacga aatccacaaa tagtcttcat atgatacaat tcctccccca tgacctgtat
     3001 aatcagttcc atcactgttc ttaattaata ctttctttat cgaagagcca gtttttgcat
     3061 ctataagata tattctggaa gcactgttgt catccatata tccgctaatt gctataatat
     3121 ttgctttttc tatataacaa agaccctgag gtataaattt atcatttaat cccggtattt
     3181 catattcttt ttccgctgtt gtataaaatt cctgatactt atcatattgt ctgtcttctg
     3241 aaggtttgca tcctgttatg cttgctataa gtattacagt gaaaagtaaa attagtattt
     3301 tcttcataat aaatccccta tgcatacaaa ttgtatattg atattataac actgttaagt
     3361 gtttacataa gcatttgact gtattttaaa tctaatattt gtactcttaa ttaaatattt
     3421 ttaatttttt tctagaaact ttattttcaa aaatgaaata ttgttatgtc gaatatgcat
     3481 atatgtatcc ctttaatggg ggaaaaagca ataaatccca tcttaaaatg aaattgttaa
     3541 taaaaatacc tgcaatattg aaaaaaaatg ttgacatctc ttataatgcg tggtattgtt
     3601 gtaaaaaata aattcaagga gtaagaaatg ccggatttat ccgtaatcat cattataagc
     3661 ttagcactat tactatcagt cgtcgtattg ttaggagttg tcgtcatact aattatacgc
     3721 aatactgtag gtggatcata taagctgaat cggacaaaaa aagtggcata accgaataaa
     3781 agcaaatgac aatcctacag gaattaagac tgtaggattt tttttataat aaaggagggg
     3841 acatgaaaga aatcggaaca aagattataa tagacacact catagagcaa ggatgcgata
     3901 cagtattcgg ttatccaggt ggagcagtct taaatattta tgatgaactt tacaaaaact
     3961 cagacaagat aaagcatgtc ataagcgcac acgaacaggg tgctgcccat gcagcagacg
     4021 catattcgag agtaactgga aaagctggag tagtcatatc aacttctggt cctggtgcga
     4081 caaatcttgt aacaggtatt gctaatgcat atcttgattc atctccaatt gttgctatta
     4141 cagggaatgt tgcattacct cttttaggta aggacagttt tcaggaagtg gatattactg
     4201 gcatcactat gccaataaca aagcataact atatagttaa ggatattaac gaactagctg
     4261 acgttataag agaagctttt atgatagctc agtcggggcg tccaggtcct gtattgattg
     4321 atattccaaa agatattcag acagctatgt gtgaatatga accaaaaaaa cctcaaagaa
     4381 aaggcattaa atatgaggta actgaaaaac agttagatca ggtagctaaa gtgatagctg
     4441 aatcagaacg cccatacata tattgtggcg gcggtgttgt tatatctcaa gctggagagg
     4501 aaattgctaa acttgcacag aaaattgatt ccccaatagg tttttctatg atggggctgt
     4561 ctgctgtatc agcggacaat ccatattgtc ttggaatgac tggtatgcat ggcagatatg
     4621 cttcaacaaa agcgaattcc gaagctgatc tcataattgc agctggagtt cgtttttctg
     4681 atcgtgcaac tggtaataag agcagatata ctcaaaaatc aaaagttatt catattgaca
     4741 ttgaccctgc cgagatagat aagaacatct cggcatattt cggccttatt ggcgatgtga
     4801 aaactgtatt gtctaaacta ttgtctaaag tcaaacaaca aaaacataca caatggatga
     4861 atcagattga gataatgaga gaagaagagg ctgctcagtt tgtaaaagat gctgtaatgc
     4921 atccaaaaga tataattgaa aaggttatgg agtatactca agacgatacc gttattgcga
     4981 ctgatgttgg acagcatcag atgtgggtag cgcagtatta cagatttaaa aaacctcgta
     5041 catacctgac atcaggaggt ttaggtacga tgggttttgg aatgggtgcg gctataggcg
     5101 gatgttttgc atctggctgt aaaagaacca ttttatttac aggtgatggc tcttttggta
     5161 tgaacttaaa tgaaatggct actgctgttt ccaataatct gcctttagtt ataataatat
     5221 tcaataacgg agtattagga atggtaaggc aatggcagaa tacattttat gatcaaaggt
     5281 attcgcaaac aactttgaat agaaaaactg attttgttaa acttgcagaa gctttcggtg
     5341 catctggata caaagctatg gatatggata tgtttgagga tgctttaaaa aaagcttttg
     5401 ctacagatgg tccggtggtt attgattgtt caataaataa ggatgaaaaa gttttgccta
     5461 tgattccacc aggcggttct atagataata taataataag gtagaagagg agattctgat
     5521 gaaaaaagaa caattcatat taagtgtttt agttgcaaat catgcaggtg tgcttacaag
     5581 gatatcaggg ttgttttcca gaagaaacta taatatagat agtttgaatg tagcggagac
     5641 agaagacgca aaactttcaa gaatgactat tatagcaaga ggagatgaag caaccagaca
     5701 gcagatagca aaacagttgt caaaacttca tgatgttaag aaaatagata tcctgcctcc
     5761 ggaaaatacc gtattaaggg agcacttgct tttgaaaatc aaggttacaa agaaaacaag
     5821 aggtgagata gttgaatcag tgaatatctt cagaggcaga gtagtagatt ttaattttga
     5881 ttcacttaca gttgaagtta caggagaaac cgacaaatta gatgcgttta taagattcgt
     5941 atcacagttt ggaatattgg aaatgtgcag agcaggagct ttagcagtca aaagaggagt
     6001 tttgatgagt gctgagcaaa ctgatgaata ttaaggagga ttagaaaaat ggcagtaatg
     6061 tattatgaga aggattgcga catgtcgctt ttaaagaaca agacagtagc ggttattggt
     6121 tatgggagtc agggtcatgc tcatgctctt aacctaaaag acagcggagt taatgtaatc
     6181 gtaggattat atgaaggctc aaaatcaatg gagaaagcaa gacaggctgg cttaaaagca
     6241 atggttacag ctgaagctgt taaacaggca gatttgacaa tgattttagt aaatgatgaa
     6301 ttgcaggcgg ctatttacaa aaaggatatt aaggataacc tgaaaccagg ctctacttta
     6361 gcatttgcac atggttttaa tatacatttt aaccagatta taccaaggga tgatattaat
     6421 gtaataatga tagcacctaa agggcctggc catacagtta gaagccagta tgaagagaat
     6481 aaaggtgttc catgccttat agccgtttat caggattata cgaaaaatgc aaaacagatt
     6541 gcccttgcat acgcaaatgg aattggcgga gcaagagcgg gagtattgga aaccactttt
     6601 aaggaagaga cagaaacaga tctttttgga gaacaggcag ttctttgcgg aggtgttaca
     6661 gctttaatga aagcgggatt tgatacttta gttgaagcag gatatcagcc agaaagcgca
     6721 tattttgagt gtatacacga aatgaaactc ataattgatc ttgtagtttc tcaagggatc
     6781 tcctttatga gatattcaat atctgataca gctgagtatg gtgattatgt ttcaggaaag
     6841 agacttataa ctgatgatgt taagaaagaa atgaaaaaag tactaactga aatacaagat
     6901 ggaacttttg ctactaactg gattttagag aaccaggcta acagaccatc attcttggca
     6961 atgcgtcgta tgcaaaatga gcatctttca gaaaaagtcg gtgcagaatt aagagaaaag
     7021 atgagttgga aaaagtaagc aaagaaagga attatgatga acagcgataa gattaaaaaa
     7081 ggagcggaag cttgtcctaa aagatcatta ttaaagtcta tgggctatac agatagtcag
     7141 attgagaaac ccttaatagg aatagttaat tcatttaatg aaatagttcc tggccatata
     7201 catttaaaaa atattgccag agcggttaag gatggcgtgc tagcagcaca gggaacacca
     7261 atagagttta acgttattgg tgtatgtgat ggtcttgcca tgggacatgt tggcatgaaa
     7321 tactcgcttt catcaagaga agttatagca gattcaattg agtgcatggc tatggctcat
     7381 gcatttgatg cattagtatt catacctaat tgtgacaaga tagtccctgc aatgcttatg
     7441 gcagctgcaa gaatcaatgt accgagtgtt tttgtttcag gtggtcctat gctttcacta
     7501 aatgatggaa aaaatgattt ggatttgaat agcgcatttg aagcagtcgg tgcattcatg
     7561 aacaacacca tagacgaaaa ggaacttcta aggattgaga ataacagttg tcccagttgc
     7621 ggttcatgct ctggaatgtt cactgcaaac tcaatgaact gtttaagcga agctttagga
     7681 atggctcaat ggggtaatgg tactatacct gctgtatatt caaaaagaat tcaactcgct
     7741 aaaaaagcag gtgaagcaat tatgtcaacc ttgaaagaaa atatacttcc aagagatata
     7801 atgaccaaaa aggcttttga aaatgcccta actgtggata tggctttagg atgttcaaca
     7861 aattcagtat tacatctgat ggctattgct catgaagcac aagtagattt agatcttgaa
     7921 atggttaaca ggataagtga aaaaacaccg aatctgtgta aacttgcacc tgcaggtcct
     7981 tatcatatgc aggatttgga tgcagcaggc ggagttcaca gggtaatgta tgagttatgt
     8041 aagaaatcat taataaatga agatgctatg acatattcat caaaaactat gaaagaaatg
     8101 attgataatc atatatcttc aatatcaaga aatgatgtaa tacgccctat tgataaccct
     8161 tattcagata aaggagggat agctgtatta ttcggttctt tggcaccaag aggatctgtt
     8221 gtaaaaagaa gtgcagtagc acagcagatg atgaaacata ctggtccagc aagagtcttt
     8281 aacagtgaag aagaagcatg tagcgctatt tattctggaa gtattaataa aggggatgta
     8341 gtagttataa gatatgaagg accagctggg ggaccaggaa tgagagaaat gcttaatcct
     8401 acatcagcaa ttgccggtat gggtcttgat aagcatgtag ctttaattac tgatggaagg
     8461 ttttctggtg cgacaagagg tgctgcaatt ggtcatgttt cgcctgaagc agcaagtgaa
     8521 ggacctatag catatgtgca tgaaaacgac atgatatcaa tagacatacc aaataacaga
     8581 ttagatttgg aagtttcatc ttctgaattg gaacaaagga taaagacaac aactatttta
     8641 aaaaaggaaa atcttaaggg atacattgca agatatgcaa gaaacgtttc atctgcagat
     8701 aagggcgcgg ttgtttctta agtgaggtaa aaatggaaag acaaataagg atatttgata
     8761 caacattgcg tgatggagaa caagctcctt cgtgtagtat gaatactgaa gaaaaaattg
     8821 aaattgcaaa acaacttgaa attttgggag tagactgtat tgaggcagga tttccaataa
     8881 gttcagaagg agcttttcag tctgtagaaa ggatagccaa aacagtaaaa aattcacaga
     8941 taacagcatt gtgccgtgca gtggaaaaag atattgatgc agcatggaat tctataaaag
     9001 gcgcacaaag ccctatgata cacacttttt tggctacttc tgctattcac atggagcaca
     9061 aattaaagat gtcacctgaa aaagtacttg aaaacattga ttatgcagta cgatatgcta
     9121 agaaatactg taaggatata gagttttcag cagaagatgc aatgagaagc gataaggatt
     9181 ttttagtaaa ggcatttaat gttgcaataa aagctggggc tacagtcata aatgttcctg
     9241 atacagtagg atatatgcta ccagaagaaa tttatgacag aatcagatat ctttttgata
     9301 atgtcaatgg catagaagat gtaatagtat cagtgcattg ccacaatgat cttggaatgg
     9361 ctgttgctaa ttcgcttgca ggagtgaagg ctggagcatc ccaggtggaa tgtactataa
     9421 acggtttggg ggaaagagcg ggtaatgccg cgcttgaaga gatagtaatg gcattaaaaa
     9481 caagaagacc actatttaat gcatatacaa atatagatac caaacagata agcaggtcaa
     9541 gtaaacttgt ttataacata atcggacaga ctgcgcctat aaataaaccc atagtcggag
     9601 caaatgcatt tgctcacgag tcaggaattc atcagcatgg agttctttca gcaagagaga
     9661 cctacgaaat tatgaaagct gaggatcttg gcatagtcga gaataagctt attattggaa
     9721 aacactccgg taaacatgct atagcagaca ggctnnnnnn nnnnnnnnnn nnnnnnnnnn
     9781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9841 nnnnnnnnnn nnnnggacct ataaatgcag catacaattc cattaacagt cttactaaca
     9901 atatatgtga aagattggtt aactattcga tacattcagt atctgatggc aatgatgcat
     9961 taggagaagt tacagttaaa ctgaaatcca aagatcttat tgttacagga agaggattat
    10021 caacaaacat aattgagtca agtatcatgg catatataaa tggtattaat aagatacttg
    10081 agatggaata gcgggaggac tgatgaaatg tctaaaagaa aaatcgaaat atttgattct
    10141 acgttgcgag atggcgctca gggagagggc atatcttttt cagtaagcga caaattaaat
    10201 attgtaaaag ctttggattc atttggtgtt gactatatag aagcaggtaa tccgggttct
    10261 aatcctaaag atatggagtt tttcgagaaa gcatctaatc ttaagttgaa taatgctaaa
    10321 ttatgcgcat ttggaagcac agccagaaat ggaatacatc ccaaagagga caagaatgta
    10381 aaagctcttt tatcagcagg aactgaaact attgttattt tcggtaaatc atgggatctc
    10441 cacgttgaga aagtgttgaa catttctcta gaagacaact tgaagattgt tgaagataca
    10501 ataagttatt taaagtctga aggtaagtat gtaatatttg atgctgaaca tttttttgac
    10561 ggatataaat cagatactgg ctattcaatg aaagttctgg aatatgcaat aaaaggcgga
    10621 gctgatgttt tagcactttg tgatactaac ggcggatgtc tgccaaatga tgttgaaagt
    10681 ataacaaaag aggtcattaa aagatttcca ggaataaaaa tatcaataca ctgtcataat
    10741 gacactggtt gtgctgttgc aaatagtata atagctgttg gagcaggagc atcacaggtc
    10801 cagggaacat ttataggtgt aggtgaaaga tgcggtaatg ctgatttatc agttataata
    10861 ccaaatctga aattgaaaat gggatatgac tgtaatggtg atatttccca gctatatgct
    10921 acttcaagaa gaatagcaga tatatcaaac acgcagatag ataataacag accatatatt
    10981 ggaaagtctg cctttgcaca taaaggcgga atgcacattg atggtgtttt aaagataaat
    11041 agcagttttg agcatgtgaa tcctgagtca gtagggaata aaagaaaact tttaatgagt
    11101 gaagttgccg gtagaagcac tctaatagcc aaagcctcaa gcatagttcc taatttatcc
    11161 aaagattcca aagaagttgc attaatactg gaaaaactca aagagatgga gcattacggg
    11221 tatcattttg aagcagctga tgcatctttt gagttgttgg ttaaaagaac tatgggactc
    11281 tataaacctc attttaatct agttctatat aaaacaatgg gagagtttcc agtacccgtg
    11341 ggaggaatgc cagcttcagc gacaataaaa gtagaagtag atggcaaaga agagataact
    11401 gctgtaatgg gcaatggccc tgtaaatgct ttggaccttg cattaagaaa atctcttaca
    11461 gttttctacc ctgctataaa ggatatccga ctgactgact ataaggtccg tgtacttgaa
    11521 gaaaatagta caacagctgc aaaagttagg gtacttatag aaacagcaga tgagaaacga
    11581 agttggtcca caataggagt atcaaatgat attattgaag caagtcttat agcattagtg
    11641 gattcaattg agtataaact tgcaatgaat gaataatgga ggaataaata tgacatctat
    11701 gaccatgaca caaaaaatac ttgcaacaca tgctggtatg aatgaagtga cagctggaca
    11761 gctgatactt gcgaatgttg atatggtact gggaaatgat ataacatcac cagtagcaat
    11821 aaacgaattc gggaaaataa agactgataa agtttttgat aaagaaaaga ttgcactagt
    11881 aatggaccac tttgcaccta ataaagatat ccaggcagca cagcaatgta aattgtgcag
    11941 agaattttct aatgagaagg aaataactaa tttttacgat actggtgata tgggtataga
    12001 acacgcttta ttgcctgaaa aaggacttgt gacatcaggg gatatcgtgg taggtgctga
    12061 ctctcataca tgtacatatg gagcgctggg agccttttca acaggtgtag ggtcaacaga
    12121 tatggcctgt gctatggcta caggtaaagc ctggttcaaa gttccttctg caatctgttt
    12181 caatcttaca ggtaaactaa atgaatatgt atcagggaaa gacgtgattc tgcatataat
    12241 tggaatgatc ggtgtgtctg gtgcgctgta taaatcaatg gaatttaaag gttcaggatt
    12301 atctaatctt accatagatg acagactatg tatggctaat atggcaattg aagcgggagc
    12361 aaagaatggg atttttgagg ttgataatat tacacttgaa tatcttagta gtgtaggagc
    12421 aaaaaaacct gtaatttttt cagctgataa gaatgctgtt tatgacaaaa catatgacat
    12481 tgatcttagt tccataaaat caactgttgc tttcccacat cttccagaaa atgcaagaac
    12541 ttttgacaat atggaggata tcattataga ccaggttgta ataggttcat gtacaaacgg
    12601 cagattgtca gatcttattg cagctgcaga aattataaag ggtaaaaaag tagctaaaaa
    12661 tgtcaggaca attattatac ctgcaactca gaagatatat ttgcaggcta tggagatggg
    12721 tttgttaaag atattcattg aggctaactg tgctgtgtct acacctacct gcgggccgtg
    12781 tcttggaggt catatgggaa ttcttgcaaa aggtgaaaga gctgttgcaa ctaccaatcg
    12841 taattttgtt ggaagaatgg gacatcctga atcagaagta tatctggcga gtcctcaggt
    12901 agcagctgca agcgcgatag ctggtaagat agctcatcct tatgatgtaa tgaagaaggg
    12961 agaatagata tgtcagtaag aggaaaagta attaaatttg gcaataatgt tgacactgat
    13021 gttataatac ctgcacgtta tctgaatact accgacaagt tgacacttgc gtctcattgt
    13081 atggaggata tagataagac atttgtaaac agggtaaata aaggggatat aatggttgca
    13141 ggtaataatt ttggatgtgg gtcatctaga gaacatgcac ctattgcaat taaggaaagc
    13201 ggtatatcat tagttattgc aaaagatttt gcaagaatat tttatcgaaa ctcaattaat
    13261 atcggtcttc cgataattga atctgttgaa gcatctgaac agattaatga aggtgacgaa
    13321 attgaagcag attttgaaaa aggagtaata ataaatcata cgcagaataa agaatatgtg
    13381 attgaacctt tccccgattt tatacgcaag attattcaag ctgggggatt aatagaagct
    13441 gtaaacaaag gtatgctagc agactgattg aatttatttg acaagaagaa gaggaaaata
    13501 tgaaattaaa aatcggatta gtaaaaggtg atggaatagg tccagaaata gtggaatgtg
    13561 ctataaaagt gcttgataag gttttagata aaaccgaata ttctgctgtt tatgaagaat
    13621 tatgtgctgg aggagtagct atagataaat atggtgtgcc actgcctgat gagacaataa
    13681 gtaaagcttt agaatgcgat agtgttcttc taggagcggt aggtggtcca aaatgggata
    13741 ctttgccagg tcatttaagg cccgaaagag cccttttagg attaagaaaa gaattaggtc
    13801 tttttgctaa tatacgtcca gttaaacttt atgatgcttt gaaagacaga tgtttattaa
    13861 aggattctga tgagatagac attgttatag ttagagaact tacaggtggt atatattttg
    13921 gaaaaagagg aagaaatcag acaactgatg gagtagaagc atttgatact gaaacttata
    13981 atgaaaagga aatagaacgc ataggccgtg tagcatttga gtatgcaatg aaaagaagaa
    14041 aaaaagttac cagcatagat aaagcaaatg ttttggaaag ttccagacta tggcgtgaag
    14101 ttatgcataa cttaaaaaag cagtatcccg aagttgaata tgcagacatg cttgttgata
    14161 atgcagctat gcagcttatt aggaatccaa aacagtttga tgttatggta gcttctaata
    14221 tgtttggtga tatattatct gatgaagcat cacagcttac cggttcaata ggcatgcttc
    14281 cttcggcttc actgggtaat acaaaaaaag gattatatga acctattcat ggttctgcac
    14341 cagatatagc aggcaaaaat ctagccaatc ctattgctac agtcttatca gtatcaatga
    14401 tgctgtctta ttcatttaat cttgataatc tagccaagaa aattgataat gcagttgaaa
    14461 agacccttaa aaatggtttt tttacaaaag atattggagg agatttaact acgaatcaga
    14521 ttactgataa gatattagag tatttataac tgatccgtaa tcatttcttt ataaattctt
    14581 ttagctttat cagaaagctc attttcgaaa gaacctgtga aaacaggttt tttatttgct
    14641 cttaaataaa gtatagatgc tatccaggca ttactccatc cgctttcaac acaggcagca
    14701 ccccagccta tatcagcagc tgaactgtaa tactcccatt ttgaataatc aaaacctctc
    14761 atccatgctc cgtcaagata aggctgttta gtactcttta tctgaatttt agttaaaaaa
    14821 tcagctaata gtgtacaagc ggtattaatt tttttgtctt caagtattaa tgaagcttca
    14881 tgtaaaccta taaatgccca atttgcagta tacataaggt cagttgcagg atcagaattg
    14941 ttctgtatta acgatgcttc cgttgtgcca tagtcttcat tggttttagg tggtggatat
    15001 cttcctttgc ttacatcctt gaatccatcc cgtatagctc cgcatggctg ctgatactct
    15061 aatattccat atactgcttt ttccagccat tttttgtgtt cttctttatt tgttacttcg
    15121 tacaaaatac ataatggtag tatcattctt gctatttcag cagtaaggct gtttgtccat
    15181 attaattcat taggaaaagc atccatagta atttctattg cttttagagc ttttatatat
    15241 aactcctcta tgccagtcat cttatatacc catagataga cagcccatag atacgactga
    15301 taatgaggag caagatggat aaagtcttcg ttatagtaat ccttccatat catatctttg
    15361 aaccttccat catctaatcg cattcttctg aagcctaact taccggttgt tcgtaaattt
    15421 ccaaaaacac atttcaagat atattcatcc aaatctttga aacctaatgt atttcttaat
    15481 attaaagaag ataacatcac tcttgcatta tcatcaccat agaaagcttt tccattagca
    15541 taccagtttg taagcccgta cattgaagat gaagggtccg tattagcaaa agaaggaaga
    15601 gtaaatatgt ttgtagccat gtttttagca attttttcat attcaggatt atttgtataa
    15661 ctggcatata gtccaaactg cattgctgtt tccaggttgc aatctcctct attagacaat
    15721 cttaagaact gtcggccatc gtatgtgatg tctgattggt aaccttcaat agcaaaataa
    15781 ctatcgtcca tttttgtaag tatattatta tatgaccata tacagttttt tttgaatgct
    15841 gagatgtagc cttttttggt aatatgagca tcactttcat gcataaattc aatatcactt
    15901 tgccaggaaa gttcaatgtt aagacctaaa tgttttaata ttccttccca tattccaatc
    15961 catcgcaaat atggagaata tctccctttt ataaagtcag ataaacttgt tgtacatatt
    16021 aagatgtcct cattcccatt caagtaacct aatacaggta tggattcttt tggcatatca
    16081 tatgctaaag aatcataacc ggcagctttt gctagactta ttgatgtatc agtaatcatg
    16141 tcagagtatc tgtaccagca tccatttata tagcatatac tgtttttagc tggattagat
    16201 gagaaaaaat cattttgtat tattgctctt tcatatttta tggttttagg ttcttctgtt
    16261 tttatgtcat taatggataa tgggtattct acatatgctt ttattttctt gcttctgatt
    16321 ttatcaataa tggatttaga taattcaaaa gtagctgtag ggtaaccgtc agctaaaaag
    16381 ataatacttg agtcataatc tgcattattg atcgcatcct ctggattttt tgatattttt
    16441 ggtttatatc cactatgttt caaagcctta annnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16561 nnnnnnnnnn nttggttaaa ttcatcagtt ttttctattg tgcaaacatg accacaatta
    16621 ttaataatat gaacttttgc gtttttaagt aatgaaatat ccttctgaat tggattttta
    16681 aatagatggt cttgttttcc cattatatat ataaacggaa ttggaggatt gtttatattt
    16741 atcttattaa gaatctgcct attatcaatt agaactggta atatatctac ccacttggca
    16801 aattccacat gaccaagttt atccgattct cttacgaaaa tctctcttga tttcatgtga
    16861 ttttttccag gcatgattat atgagcgaaa aatttataca aagtaaggta aggtatgaca
    16921 tttttcagtt ttcttgcaag atagagcaaa gtttttgtta atacattaaa atgtatcatt
    16981 ccgccagcca taatcattcc taatactgaa tcaggataac acgaagcgta tgccaatgct
    17041 accaatgaac cagacgacat actgacaaaa aaggcttttt ttatattctc atagcaaaga
    17101 gattcatata tgtttgaact tattgattgt atatctagtt tgaataattt tgcagaattg
    17161 ctatgaagat ttaaaagaag aagattaaaa tattttgaat atgcttcaat ctgacctttc
    17221 catgttatat tgcttccacc aaatccgtgt ataaatacta cccatggata ccgttcattg
    17281 tttctaacaa ctgaataatc aaacattttt attacttctt ctttcatctg aggttcgttt
    17341 tttgaacctg tgatacttta agaaaatagg gaagtttttc tgattgtgta caggcatatt
    17401 tttaaataat tcctcagctt taatcttcca ctgctcagct cttttatagt ttttctgtgc
    17461 aagtgtttca gcagattcag gattgttaaa atgcgtagaa tgagcattgc cttcttttac
    17521 cacattaact ataccttctg gtacatccac taatgggcag gctcttaatg gattgctaga
    17581 gaacggctgg gcttttctaa aagctgtaaa gaaaggagat ctgagcgcat caagtaatga
    17641 aacttcatgt atgtttgaat ctgaataatg acagaatgcg catggttcta catcgccttt
    17701 ggagtttata tgtataaatc cgttacctgc tgctaaacaa ccaaaagcta aatgaccatt
    17761 attccagaaa tccacaatag tatattcatt ttttatacag taatcagaaa ttttctgctg
    17821 tacatatgct ctttgttcag cattacatgt taattcaaga tcagcatcac ttcctacagg
    17881 aacataattg aataaccagc cgaaccaaca gcctttttct ctcatataat ctaaaaattc
    17941 atcagacgca atagtcttgt agtttttgct gtgatagcat gcagagaacg cgaagccgat
    18001 atctctgctt ttaagtatat ccattgcttt tataactttg tcgtaaacac cctgacctct
    18061 tctaaagtcc gtttcttctc ttgtgccttc aatacttaaa aacatgttaa gatttcctaa
    18121 acgaaccatt tcgtcagcaa atttctcatc aatcatagta gcattagtaa aagctgcaaa
    18181 agttgtcctt cgatgtttat cacataactt tatgatatca tcctttcgca ttagtggttc
    18241 accgcctgtc atcagtacat caccgatacc taattttcgt gcctgagcga ttatatcatc
    18301 aagtttctca tatgatagat gatctgttct gctgtaatca gaagcccagc agcctttaca
    18361 ttttaaatta catgctgatg tcggatcgat taataaaact cttggtataa atgtcttata
    18421 cctccaaccg tttataagtt ctttgattgt tataaacctg ttcattgtca tgtgatcaag
    18481 aatacttttt gccataaaga attttcttga gcagtataca taaaatttgt ggtggggaaa
    18541 aggagggatt tttttctttt ccccgttaat ttttaaatat ctcatcaata ttttggtatt
    18601 gcattaacgg tttgaggtat gagtccgtca aaaacaaagt ttccgtttgc atcactacct
    18661 agtgaataaa tctcaagacc agggattatt gtatatattc ttgaaaaaac ctcttccatg
    18721 acttcaatac tttcttcagt catatactca tctggtaaat taaccgggcc tatcttaaat
    18781 acatccggat taccagtaag ctgattgttc tgtatgctta ctgtgccgga gctgtatata
    18841 tttacactgc ttggtaatag agcggataac tctgatggta tttcatcttt aagatca
//
LOCUS       DAOSXM010000037        18621 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2111
            NODE_1888_length_18621_cov_10.909943, whole genome shotgun
            sequence.
ACCESSION   DAOSXM010000037 DAOSXM010000000
VERSION     DAOSXM010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864178
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 18621)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18621)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:24:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,261
            CDSs (total)                      :: 2,229
            Genes (coding)                    :: 2,218
            CDSs (with protein)               :: 2,218
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 3 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18621
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1888_length_18621_cov_10.909943"
                     /isolate="AMR_MDS_2111"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..725)
                     /gene="groEL"
                     /locus_tag="PLQ68_06195"
     CDS             complement(<1..725)
                     /gene="groEL"
                     /locus_tag="PLQ68_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005210783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HXK72277.1"
                     /translation="MAKQIQYGIEARKSLEAGVNKLADTVKITLGPKGRNVVLDKKYG
                     SPLITNDGVTIAKEIELEEPFENMGAQLVKEVATKTNDVAGDGTTTATLLAQAIVREG
                     LKNLAAGANPMILKKGIEKAVTAAVEGIVENSSVIKGKEDIERVASVSANDVEIGKLV
                     SAAMEKVGNDGVITVEESKTMGTNLEVVEGMQFDRGYLSAYMVTDTDKMEAIIDDAYI
                     LLTDKKISNIQEILPLLEQIVQSG"
     gene            complement(745..1029)
                     /locus_tag="PLQ68_06200"
     CDS             complement(745..1029)
                     /locus_tag="PLQ68_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012062101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HXK72278.1"
                     /translation="MVIKPLCDRIVIRMLESEETTKSGIVLPGSAKEKPQVAQVIAVG
                     PGGFVEGNKVEMELAVGDKVLISKYAGTEVKLDGEEYTILRQSDVLAKVE"
     gene            complement(1256..2872)
                     /locus_tag="PLQ68_06205"
     CDS             complement(1256..2872)
                     /locus_tag="PLQ68_06205"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003528597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HXK72279.1"
                     /translation="MENTTKYIFVTGGVVSGLGKGITAASLGRLLKARGLKVAAQKID
                     PYINVDPGTMSPFQHGEVFVTDDGAETDLDLGHYERFIDEDLNKYSNLTSGKIFWNVL
                     NKERKGEYLGETVQIIPHITNEIKSFIYRVGKKTNADVVITEIGGTIGDIESTYILEA
                     IRQISLEAGRNNCLFVHVTLVPYLASSGEYKSKPTQHSVKELHSMGISPDIIVLRCND
                     HLDESIKQKISLFCNVKEDCVIENIDMEVLYEVPLVLENMNFSSLVCRELKLKTNEPD
                     LTEWKAMIKKLKNIKDEVVIALVGKYTQLHDAYLSVVESLKHAGFENNVKISVKWIDC
                     EEVTYENSKEFFSDCHGILIPGGFGVRGIEGKIAACNYARVNNIPYLGICLGMQVAVV
                     EFARNVCGFTDANSGEFDERSAHKVINLMPDQYVSIAKGGTMRLGAYPCKIKDGTLMK
                     KAYKANEISERHRHRYEFNNEYRKTLEAQGLKISGTSPDDVIVETVEIPKNDFFVGVQ
                     FHPEFKSRPNRAHPLFALFIKNAVNKKAGK"
     gene            complement(3091..4971)
                     /locus_tag="PLQ68_06210"
     CDS             complement(3091..4971)
                     /locus_tag="PLQ68_06210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HXK72280.1"
                     /translation="MPDKVYSELNFSQNLKTILAVLGCTNSDIAVYGECSASNISRIR
                     SGARVCKMNSSTMNSIVNGIYGYTEAHHYLEKLYKLMGMKSDLSPDKTKKAIVHWMYS
                     ENDNKLQKSVGNSRPGRKLPFNFYSEKLNAIMDMLDLSNVKLGKAINVDPSYISRLRN
                     GTRVPKRNSDITDRISEYVLERIEEQDKLKQLSRMMNVTMRALEDADYRKNAFFKWIN
                     DKDNIAQESAIEKFLENIDSFKPAIYSTLPDISSIINKEVLNDLRCEYVGVKGIQAAV
                     IRFLSNSISENADELWLYSDEDMSWITQDNDFLLKWTVLLGACVKNRIKIKIIHTVDR
                     DPDEMIKGLESWIPLYMSGMIEPYISRRRVDNRFSHSMFICPNKSCIEACHVKGSESF
                     GLYRYHTDHEHLSLYMKQYEMLLSTCEPLMKIYTQADLAKYISFTDNVVNEGGIVSVL
                     QSLSVGFMDKSLLDKMIAKADCNDEELEKIYSFWQSRVSLYERALQDENIREMIPLPS
                     KDSIDKGEVMLNLGTIRNDLIIPYEKEEYVEHIRNIIRTIDRYKNYTLIPLPELPFIS
                     VQIIINGDNVSLIKTDFPKISFVISNQYIVQAFKDYTARLADKYAKDKETVKKMLSEY
                     VD"
     gene            complement(5237..6586)
                     /locus_tag="PLQ68_06215"
     CDS             complement(5237..6586)
                     /locus_tag="PLQ68_06215"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HXK72281.1"
                     /translation="MNISYNKTIEVRYTADVFVAGGGPAGNAAAISCARQGKKVFLAE
                     SFSAFGGAAVTMLVPGFSVFTDGVNFLSSGIGKEVYDRLKEEAYPRYKRYCPRSIPVE
                     TLKVIYDDMVSDSKVKYLFHSNVIDSVVEKGSIEYVICSCKGSIFAVKADIYIDCTGD
                     GDLAYYSNADYEFGDKNGLVMAATLCGIWSGIDWDNVKLPDGRCLDKAFSDKVFTNED
                     RHHPGMWRIAQQTQNSQGLNNSDGIGGSNVGHIYGIDSTNSESLTEGIVKGRHQLKEY
                     RKYYKEYLSGFENCELICSASYLGIRESRRITCDYRLTVDDFKSRASFEDEIGRYCYG
                     IDIHSATNDKEGYDKFISEYTSLRYAKGESYGIPYRSLAVKGIANLLTAGRCICTDRY
                     MQSSIRVMPGCFITGQAAGIAAAILCEDKEYDVHKADIKKIQKRLVSSGAYLPNCKV"
     gene            complement(6698..8677)
                     /locus_tag="PLQ68_06220"
     CDS             complement(6698..8677)
                     /locus_tag="PLQ68_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009005295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HXK72282.1"
                     /translation="MSKQYPILFSEGQIGNLKIKNRVVMPAMGMNQSDRGFVNDAVIN
                     HYERRAKGGVGLIIVEVTCVDTPLGRNTENMLVIDDDKYIEGMKKLVDAIHKHGAKCF
                     LQISHTGRGARREYIKEQPVGPSEVAMTYSFMIGYGNEAPRSLTVEEIKTIEDKYAAA
                     ALRAKKAGFDGVEFHCTGYYLGQQFLSSTANIRADEYGGSKEKRITFHMNVINKIKKL
                     VGEDFPIAVKLTVWEMGKDGGITLAEGAYYVKRFEEAGVHAIEVMAGMWSDTPTLKDT
                     PDSGSSKGQIFPVCMLLKLVYSLKFFKKLKLKLIGGGRAADADVAENALKKKTVDFVF
                     MGKGLLAEPDWVNLVEQGKADLIRPCIGCQKCIDSQLQSGGRAICSGNAVIGRGDNIY
                     EIPSSEVKKKVVVVGAGPAGVEAARIAAMRGHSVVILEKSDKVGGQLYEAVVPKHKQN
                     LKQLIPYLKRQLEYNKIEVKFNKEATVENVLAEKPDVVITATGVLPVIPKIPGIESSN
                     VILVSDVLKGAQTGKNVVIIGGGIVGCETAEYLASDKKNVTIIEMLDEVASNMVNVSR
                     TILLGHLKLQNVVIHTKAKCVGIYDTYILVEQSDKSQIKISCDTVVIAAGYKPNNMLY
                     EQLKDKVETYNIGDSNNPDSVASSVYEGYYTALKI"
     gene            complement(8794..9333)
                     /locus_tag="PLQ68_06225"
     CDS             complement(8794..9333)
                     /locus_tag="PLQ68_06225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72283.1"
                     /translation="MSKRRLDLNRENLICLCEGNAEYDILTLLLEHEKLIFTREMLHS
                     NKLFKRMTASKVSENFLQYAYKEKPLILNFVDSKSESFKLKKPYNDMADVIKVCTSPE
                     IEILIIIDKNDISEFNKCKSNIKPSIFCKQNYKMKNIKTKGFMTDYFCDISKLVKAIE
                     KYNFKISNNEYCIYDLLKD"
     gene            complement(9315..10466)
                     /locus_tag="PLQ68_06230"
     CDS             complement(9315..10466)
                     /locus_tag="PLQ68_06230"
                     /inference="COORDINATES: protein motif:HMM:NF024700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HXK72284.1"
                     /translation="MKVLRIEYENINVFDKGFCFDMTASDRVVDDNQVQKISNCIYLQ
                     KVISLIGINASGKTTALRLIKFVMDIVARHKNLEQANIPFGIIKDDTVIKVDFVISGK
                     IFRLHTIIGLKKDSYIDIEQNYPYFFKEEIIYEKPITSVKKKADITKFDDDMIIMKRS
                     GLTEQAKALLPDNQSIVLSITRYASPYIYNSIHPISNYLIKGDDTVNENILKLYDENI
                     SSITVKDNNVRIVFKNGQDYIYDNLIDASQVLSSGTIKGMHILNQAAEVFKKGGYMII
                     DEIENHMNKKLVHMIIDLFNDCEINQNGACLIFTTHYPEILDVMERKDNIFILRRVNS
                     TPEIIKYSDYVKRNDIKKSDIILSNYIDGTAPNYENIQKVRKYICQRED"
     gene            10971..11231
                     /locus_tag="PLQ68_06235"
     CDS             10971..11231
                     /locus_tag="PLQ68_06235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72285.1"
                     /translation="MVTSRERFLNVINGIMPDRIPVKLFIADQGHFISQVYPDIDPWD
                     FPALQLKVIEIQKQLGLDVFLRMLYDLTDPLHIHMGGLDITH"
     gene            11365..12264
                     /locus_tag="PLQ68_06240"
     CDS             11365..12264
                     /locus_tag="PLQ68_06240"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HXK72286.1"
                     /translation="MYGCTKKPINNIKELEIAIKYEPKMPEYFKSEAKRKVKRMKDAV
                     GNDGIVGVWAPHGPFNNASLLIDHDVLYSLYLEDYEYYEKLMNFASDRILEYATAMDE
                     AGADVLCVGGNVAGGFVGKKYYDEYILPFEKKYISFCQKNGTPAMYHNCGKIMDLVES
                     YKELGVKIVEPFSPPPIGDTDILKAKQIVNGDYVMLAGIDQVNVLQKGTIDDVKRATA
                     TLTDIMKPGGKCIIQSAESKVSLEITGNEERDIIRNFSTQAIAGTARFNRIVTGKPTE
                     PPNIVLHFKAEGVTPYSIRYNLT"
     gene            12333..13268
                     /locus_tag="PLQ68_06245"
     CDS             12333..13268
                     /locus_tag="PLQ68_06245"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HXK72287.1"
                     /translation="MSLLKYIMKQGWKAIKKADADRIAKQPEILNTNFESCIPYTDSI
                     NPLHQLNLYYPFEYDMDKDGILPTIIDIHGGGLMYGDKELNMRYCQYLASKGFCVMGM
                     SYRLLPDTDLQGMISDIYTSIHWLEKNGPKRGFDLSKVLLTGDSAGGHLTSLIMCIQK
                     SKELQKVYSVEPFKSDISAVAISNGVCEMHDYFSFFPSLNKMIDREIVSMILGKSKKN
                     APWKDFMSFSQVMVKADKLPAVLVIGAENDPFYRQTLWLMENLTKHGSKFETILWKKE
                     DGPHLGHVFHIGHYEWQESMITNDKMLEFFRKNIF"
     gene            13292..13570
                     /locus_tag="PLQ68_06250"
     CDS             13292..13570
                     /locus_tag="PLQ68_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HXK72288.1"
                     /translation="MRKIADNIKLRLEYLKGFGAWTYHIRIPGTANIEGRWGYMKVSG
                     TIDGYEIKNLNLAPRKNEDKIISINKKIRDTIGKTGGDMVTVTLYLNQ"
     gene            complement(13617..13781)
                     /locus_tag="PLQ68_06255"
     CDS             complement(13617..13781)
                     /locus_tag="PLQ68_06255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72289.1"
                     /translation="MLFYSPAIYVDALSICAPNGRFILGSSDSFREGTPEINIKTYFD
                     TAMEYGSRFI"
     gene            complement(13893..15167)
                     /locus_tag="PLQ68_06260"
     CDS             complement(13893..15167)
                     /locus_tag="PLQ68_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015519750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4143 domain-containing protein"
                     /protein_id="HXK72290.1"
                     /translation="MQRKLYRPRIIDKTIIEYLAVFGAICIEGPKWCGKTWTSSFHSK
                     SEIFLSDPNGNFQNRTLAQTAPSLVLEGDTPRMIDEWQEVPMIWDAVRHFVDQRPGKG
                     QFILTGSSTPNHKGILHSGAGRIASLRMRPMSLYESGDSLGNISLEQLCKGILNPCKT
                     DQLDLRKIIDLILRGGWPESIKLLPKQAMLLPKAYLSAIINNDVYRVDGIKRDLDKIK
                     LLLRSLARNESTTVSNNTLIRDIKKIDDEDIDTNTVSSYLNIFERLFIIENQLPFASS
                     IRSKVRVRQQQKRHFTDPSLACALLSITPDMLINDLETLGFLFEAMCERDLNIYAESF
                     GARLYHYQDYENREIDAVIQLSNGNWCAFEIKLGANQIDDAARNLLSIKKAIEEEDGK
                     APCSLCVICGMSNAAYRRDDGVFVVPITELKN"
     gene            complement(15377..16294)
                     /locus_tag="PLQ68_06265"
     CDS             complement(15377..16294)
                     /locus_tag="PLQ68_06265"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HXK72291.1"
                     /translation="MIYFTHDIHDWISWSKVFQDILAWETLIEYIFRIEDLPYAKIEH
                     LTPGTNAVFKSGDYVVKIFAPKESGMDMYSDYTTEKFGLVRTESLKIPVPRLIRSGVI
                     HDKYEFMYMVMEYVDAEEMVKVEESFNDLQKEKIGRKLRVICDRMNTPCEKFNAIDVI
                     NRALKAKCWNSFSISFQRQRLDWLKKYDLKDKVFVHGDLNPDNILIDKDMNIYIIDFA
                     DSVQAPCEYELAALLCETFEFEYPYMKGFFGADYDVSDLTDKIFQTLLLHDFGENIIK
                     NNFGNPEMITELSDLHKRIYSAISNKKVC"
     gene            complement(16335..17492)
                     /locus_tag="PLQ68_06270"
     CDS             complement(16335..17492)
                     /locus_tag="PLQ68_06270"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HXK72292.1"
                     /translation="MKKEMTSRQRQINAIRGKEVDRIPCSPRIGAYLKSRYLSHGVET
                     VINFNKIYKHDPVYQMMPGLVNPVDSVYFDSSYTKDIKVDYKETANGEMKIVERTIST
                     PAGKLKDIIHVPPSNGKYGVTPNPVKREFLIKDASDIEKLRYVLPDPKKHLNLVNFNE
                     TEDKLKNEGLTQLFLRSPLDHLAGDARGIENLMIDYYDNMELFDKIMDFFTEYVMDTT
                     KVCLEKGVVNFFVSWYYSSLSTGWSPEILRQKFIPVLRKHIDLIHSYDATYDFYDDGK
                     MMDILEDYISCKPDVIETLTPPTVGDIDLVKAKKLAGDKVTLKGYVDIIYVMQKGTAK
                     DVRDTVIDALSICAPNGRFILGSSDSFREGTPEINIKTYFDTAMEYGSRFL"
     gene            complement(17669..18193)
                     /locus_tag="PLQ68_06275"
     CDS             complement(17669..18193)
                     /locus_tag="PLQ68_06275"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HXK72293.1"
                     /translation="MTVGEKIYMFRKQKGWSQEQLADKLSITRQTLSKWELNTSVPDI
                     ENVLKISTLFNVTTDFLLKDEFGDAEEYARTINVSDTVEKFKLSKKAVQLLDDKGYVG
                     AYYISVKYAIGLVVAVFVCFANLSVLSNIAPLNDFPKQAFIIPVVALLIGIFLLIKAI
                     ICFFVGYRLKRCKE"
     gene            complement(18308..18562)
                     /locus_tag="PLQ68_06280"
     CDS             complement(18308..18562)
                     /locus_tag="PLQ68_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020816606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72294.1"
                     /translation="MSCYYVISVRIDNRVNVSLKLQEVLTENGCKIKARLGLHEVSED
                     VCANDGLIILQPCGELKDVEQLVKDLNKLEGVKAKLIDLN"
BASE COUNT         5780 a         3637 c         2719 g         6485 t
ORIGIN      
        1 cctgactgaa ctatctgctc taaaagagga agaatctctt gtatatttga tatcttctta
       61 tctgtaagaa gaatatatgc atcatcaatt atagcttcca tcttatcggt atctgttacc
      121 atatatgctg aaagatatcc tctgtcaaac tgcattcctt caactacttc aaggtttgtt
      181 cccattgttt ttgattcttc aacagttata actccgtcat ttccaacttt ttccattgct
      241 gcagaaacta atttgccaat ctcaacatca ttagcagaaa ctgaagcaac tctttcgata
      301 tcttctttgc cttttattac actactgttt tcaacaatac cttcaactgc agctgtaact
      361 gccttttcaa tacctttttt aagaatcata gggttagcgc ctgctgctaa gtttttcaat
      421 ccttctctga ctattgcctg tgcaagaaga gtagcagttg ttgtgccatc acctgcaaca
      481 tcatttgtct ttgttgcaac ttcttttaca agctgagcgc ccatattttc aaaaggttct
      541 tcaagttcaa tttcttttgc aatagtaaca ccatcatttg ttataagtgg tgaaccgtat
      601 ttcttatcaa gaacaacatt tcttcctttt ggtcccaatg tgatctttac tgtatctgcc
      661 agtttgttaa caccagcttc aagtgatttt ctagcttcga ttccatactg tatctgttta
      721 gccatatctc atactctcct tttcttattc aacttttgcc aatacatctg attgcctgag
      781 gatagtatat tcttctccat caagctttac ttctgttcca gcatatttgc tgatgagaac
      841 tttatcacca acagcaagtt ccatttcaac tttgttgcct tcaacaaatc cgccaggacc
      901 tacagcgata acctgagcta cctgaggttt ttctttagct gatccgggaa gaactatacc
      961 gcttttggtt gtttcttctg attccaacat tctaattact attctgtcgc ataatggctt
     1021 aattaccata acttacctcc gtaattttat ttattttagc actttgtgct tttataccta
     1081 agttttcaac ctctcaagta ttttttagca ctctcgtgct ttgagtgcta aagatattgt
     1141 agtttacata ctacattgtc gcaaactgca aaattcccca tattcaatgt ttatattgga
     1201 ttatctctct aatactatgt atttctcatt aatgctggtt atttctcgat tttacttatt
     1261 ttccagcttt tttattaaca gcatttttaa taaacagcgc aaatagcgga tgggcacgat
     1321 tcggacggct cttaaattcc ggatggaact gtacacctac aaagaaatcg ttttttggaa
     1381 tctcaacagt ttcaacaata acgtcatctg gagatgttcc gcttatcttg agaccttgag
     1441 cttccaatgt ttttctatat tcattattaa actcatatct gtgtctgtga cgttctgata
     1501 tctcatttgc tttgtatgct ttcttcatca gtgttccatc tttaatctta catggataag
     1561 cacctaatct cattgtacca cccttagcta tgcttacata ctggtctggc ataagattta
     1621 taaccttgtg tgcgcttctt tcatcaaatt caccggaatt agcatcggta aatccgcata
     1681 cattccttgc aaactctaca accgctacct gcatacctaa acatatccca agataaggaa
     1741 tgttatttac tcttgcataa ttgcatgcag ctattttgcc ttctatgcct cttacaccaa
     1801 aacctccagg tataagtatt ccatgacaat cagagaaaaa ttcttttgaa ttttcgtatg
     1861 ttacttcttc acaatctatc cacttaaccg atatttttac attattctca aatcctgcat
     1921 gcttaaggga ttcaactaca gaaaggtatg catcatgaag ctgggtatat tttcctacta
     1981 gtgctattac tacttcatct tttatatttt tgagcttttt gatcattgct ttccattcag
     2041 taagatcagg ctcatttgtt tttaatttaa gctctctgca gaccaaagaa gaaaaattca
     2101 tattttcaag aactaaaggt acttcataca gcacttccat gtctatattt tcaattacac
     2161 aatcttcttt gacattacaa aatagtgaaa tcttctgctt tatgctttca tccagatgat
     2221 cattacatct aagtacaata atatctggtg atattcccat tgagtgcaat tcttttacag
     2281 aatgctgggt aggttttgat ttatattctc ctgaacttgc aagataaggt accagtgtta
     2341 catgaacgaa taagcagttg ttcctgcctg cctctaaaga aatctgcctt atagcttcca
     2401 atatatatgt gctctctata tctcctatag taccgccgat ttcggttatg acgacatctg
     2461 cattggtctt cttacctaca cggtaaataa agcttttaat ctcgttggtt atatggggga
     2521 taatctgtac agtctcacca agatattccc cttttctttc tttgttaaga acattccaga
     2581 atatctttcc agaagtaaga ttagaatact tattcaggtc ttcatcaata aatcgctcat
     2641 agtggcctaa atccagatcg gtttcagcac cgtcatctgt aacgaaaacc tcaccatgct
     2701 gaaatgggct cattgtccca gggtcaacat ttatatatgg gtcaatcttc tgtgcagcaa
     2761 cttttaaacc tcttgcttta agcagtcttc ctaatgatgc tgcagttatt cccttaccaa
     2821 gaccggatac tactccgcct gtaacaaaaa tgtatttcgt tgtgttttcc ataaactttt
     2881 ccccctaaag actgggtata aatatatatc caatcaaata tatcaaatcg cataaaatgc
     2941 ggattatccc acctggtagt gatgaattaa cctaacgata atattatcac ttttactgcc
     3001 ttcattccag taatttttta taaaaaaaga gattttttca gtttttttct tctgggttaa
     3061 atgtgtaagt catataactg aactttcaga ttaatctaca tactctgaaa gcatcttttt
     3121 taccgtctct ttatcttttg catatttgtc tgcaagcctt gctgtatagt ctttgaaagc
     3181 ctgtactata tactggtttg aaataacaaa ggagatttta ggaaagtctg ttttaattaa
     3241 tgagacatta tctccgttta taattatctg aacgctaata aagggaagtt ccggtaaagg
     3301 tattaaggta tagtttttat acctgtcaat agttcttata atattcctta tatgttccac
     3361 atattcttct ttttcatatg gaataataag atcatttctg atagttccaa gattcagcat
     3421 gacttcgcct ttatctatac tgtcttttga aggtagcggt atcatttctc ttatattttc
     3481 atcctgtaat gctctttcat acaatgatac tctgctttgc cagaaagaat atatcttttc
     3541 taattcttca tcattacaat cagcttttgc tatcatttta tctaaaagag acttatccat
     3601 aaaccctaca gacaatgact gcaaaactga cactattccg ccttcattta caacattatc
     3661 tgtaaaggaa atatacttgg ctaaatccgc ttgggtatat atcttcatca atggttcaca
     3721 agtcgacaaa agcatttcat attgtttcat gtataaacta agatgctcat ggtctgtatg
     3781 atatctgtac aggccaaagc tttcacttcc tttaacatga caagcttcta tgcatgactt
     3841 attgggacaa atgaacatgg aatgactgaa tctattgtcc actcttcttc tgcttatata
     3901 cggctctatc atacctgaca tatatagcgg tatccagctt tccagtccct tgatcatttc
     3961 atctgggtct ctgtctactg tatgtattat ctttatcttt attctgttct ttacgcatgc
     4021 tcctagtaat actgtccatt taagcaaaaa atcattatcc tgtgtaatcc atgacatatc
     4081 ttcatcagaa tatagccata attcatcagc attttcacta attgaattac ttaaaaatct
     4141 aattactgct gcttgtattc cttttacacc tacatattcg catcttaagt catttaaaac
     4201 ttccttatta attatactgc tgatatcagg cagagttgaa tatattgcag gtttaaatga
     4261 gtcaatattt tcaagaaatt tttctattgc tgattcctgt gcaatattat ctttatcatt
     4321 aatccattta aaaaatgcat ttttcctata atctgcatct tctaaagctc tcatagtaac
     4381 attcatcatt ctggaaagct gtttaagttt gtcctgctct tctattctct caagcacata
     4441 ttcagatatc ctatcagtaa tatcactatt tcgttttgga accctggttc catttctaag
     4501 cctgcttata tatgatgggt caacattaat tgctttaccc agttttacat tagataaatc
     4561 cagcatatcc ataatggcat ttagtttctc actatagaaa ttaaaaggca gttttctgcc
     4621 tggcctgcta tttccaacag atttttgaag cttattatca ttttctgaat acatccagtg
     4681 cacaatggct tttttcgttt tatctggtga taaatcgctt ttcatcccca ttagcttgta
     4741 tagtttttct aaataatggt gagcttcggt ataaccatat attccgttaa caattgagtt
     4801 cattgtactt gaattcattt tacatactct ggctcctgaa cgtattctgg atatatttga
     4861 agcagaacat tcaccatata ccgctatatc ggagttcgta catccaagta ctgctaaaat
     4921 agtctttaaa ttctgcgaaa aattcagttc tgaatatact ttatctggca taatcctaaa
     4981 cactcctcca ataaatctat attatactaa tcaatagtat ttggcaatgt tttgtcattg
     5041 ccataattgt gacaaatata tatttataag atataagtga taatatatat atgcaaacgg
     5101 ggtgatatac aagctaacca gcaagtatat gtcatttgtc taaaaagagg aatcaaaaaa
     5161 ggttttgcat gaagcaaaac aaatgttatg aaagagctgc gggggctctg acataacatc
     5221 ttttttttaa tgaaaattat actttgcagt ttggcagata tgcccctgat gagacaagtc
     5281 ttttttgtat cttttttata tctgctttat gtacatcgta ttctttatct tcacataata
     5341 tggccgcggc aatccctgct gcctgacctg ttataaaaca tcctggcata acccttattg
     5401 aagactgcat atatcggtct gtacatatac accgtcctgc agttaataaa tttgcaattc
     5461 ctttaaccgc caaagacctg tacggtatac cataactttc tccttttgca tatcttagtg
     5521 aagtgtattc agatataaat ttatcatatc cttctttgtc gttagtagca gaatgaatat
     5581 ctatgccata acagtatctg cctatctcat cttcaaaaga tgctctgctt ttaaaatcat
     5641 caacagttaa acgataatcg catgttattc ttctgctttc ccttatacct aaatacgatg
     5701 cagagcaaat aagttcacaa ttttcaaaac ctgaaagata ttccttataa tactttctgt
     5761 attcttttag ctgatgacgt ccttttacaa ttccttctgt taaagactca gagtttgtgg
     5821 aatctattcc gtatatatgt cctacatttg aacccccaat accatcagaa ttatttaaac
     5881 cttgactgtt ttgtgtctgt tgtgcaatcc tccacattcc tggatgatgt ctgtcttcat
     5941 ttgtaaaaac tttatctgaa aaagccttat caagacacct tccatcagga agctttacat
     6001 tatcccagtc aatacctgac catatcccac aaagcgtagc agccataact aacccgtttt
     6061 tatcaccaaa ttcataatca gcatttgaat aatatgctaa atcaccgtct cctgtacagt
     6121 caatgtagat atctgcttta acagcaaaaa tagagccttt acaggaacaa ataacatatt
     6181 caatacttcc tttttcaact acagaatcta tgacattgga atgaaacaga tattttactt
     6241 ttgaatctga aaccatgtca tcataaatta ctttcaaagt ttcaacagga attgaacgcg
     6301 gacaatatct tttgtatctt ggatatgctt cttctttcaa tcggtcatat acttccttgc
     6361 ctatgccgga agacagaaag ttcacaccat ctgtgaatac agaaaaccca ggaacaagca
     6421 ttgttactgc tgctccacca aaagcagaaa atgattccgc aagaaaaact ttctttccct
     6481 gtcttgcaca acttatagct gctgcatttc ctgctggacc tccgcctgct acaaaaacat
     6541 cagcagtata tctaacttct atagtcttgt tatatgatat gttcatggtt acactccttg
     6601 atatataatg agaaaatatg atttataaaa ataaacgaaa caataacctt attatatcaa
     6661 ataaagtgtg cctgcaaaat tgtatcaaaa tttaaagcta tatcttcagt gcagtgtaat
     6721 atccttcata aacagatgat gctaccgaat caggattatt gctgtcacca atattgtatg
     6781 tttctacttt atctttaagc tgctcgtaaa gcatgttatt tggtttgtat cctgctgcaa
     6841 taacaactgt atcacaggaa attttgattt gacttttatc tgactgttct accagtatat
     6901 atgtgtcata aataccaaca cattttgctt ttgtatgtat tactacattt tgcagcttca
     6961 aatgtcctaa aagtatagtt cttgaaacat taaccatatt acttgccact tcatcgagca
     7021 tctcgattat tgttacattc tttttatctg atgcaagata ttcagctgtt tcgcaaccaa
     7081 caattcctcc gccgattatt acaacattct ttcctgtttg agctcctttt aaaacatcag
     7141 atacaagaat gacattgctg ctttctattc ctggtatttt cggtattaca ggcagaactc
     7201 cagttgctgt tataaccaca tccggtttct ctgctaatac attctctact gtagcttctt
     7261 tgttaaactt gacctctatt ttattgtatt caagctgtct tttcagataa ggtattaact
     7321 gttttagatt ctgtttatgt tttgggacta cagcttcata aagctgcccg cctactttat
     7381 ctgatttttc gagtatgaca acactgtgac ctctcattgc agctattcta gcagcttcta
     7441 ctcctgccgg tcctgctcct actacaacta ccttcttttt aacttctgat gacggaattt
     7501 catatatatt gtcccctctt ccaattacag cattaccaga acagattgca cgacctccgc
     7561 tttgaagctg ggagtcaata catttctggc atcctatgca aggccttata aggtctgctt
     7621 ttccctgctc taccagatta acccagtcag gttcagctag aagacccttg cccataaaca
     7681 cgaaatcaac tgttttcttt tttaaagcat tctcagcgac atcagcatct gcagcccttc
     7741 ctccgccgat taattttaac ttgagttttt taaagaactt aagactgtac acaagcttta
     7801 ataacatgca cacaggaaat atctgccctt ttgatgagcc gctgtccggg gtatccttca
     7861 aagtcggggt atcactccac attcctgcca ttacttcaat agcatgaact cctgcttctt
     7921 cgaatctttt tacgtaatat gcgccttctg ctaatgttat accgccatct tttcccatct
     7981 cccagactgt tagcttaacc gctataggaa aatcttctcc tacaagcttt tttattttat
     8041 taattacatt catatggaaa gtaattcttt tttccttgct tccaccatat tcatctgccc
     8101 taatgttcgc agtagaagaa aggaattgct gtcctaaata gtatcctgta cagtgaaatt
     8161 ccactccgtc aaatccggcc tttttagctc ttaaggcagc agcggcatat ttatcctcaa
     8221 ttgtctttat ctcttctact gttaaagacc tcggggcttc attaccataa ccgatcataa
     8281 aagaatatgt catagcgact tctgaaggtc ctactggctg ctcctttata tactctcttc
     8341 ttgctcctct acctgtgtgg ctgatctgca aaaaacactt tgcgccatgc ttgtgaattg
     8401 catcaacaag ttttttcatg ccttctatat atttgtcatc atcaataaca agcatattct
     8461 cagtatttct gcctaatgga gtatcaacac atgtaacttc aacaattatt aatcctactc
     8521 cgcctttagc tcttcgttca taatgattta tgacagcatc atttacaaat cctctgtctg
     8581 actgattcat gcccattgca ggcataacta cacggttttt tatcttaaga ttacctatct
     8641 gaccttctga aaatagtatt ggatactgtt ttgacatgac acacctccat taagaaaata
     8701 taaaaagtga acttagttca ccatttaaat atatatatta atctaaattt agtcaattct
     8761 aaagcatata gcgaaataag aaatataata ttactaatcc tttagcaaat catatatgca
     8821 gtactcatta ttgctaattt taaaattata cttttctatc gctttcacta gtttggatat
     8881 gtcacaaaaa tagtcagtca taaatccttt tgtctttata tttttcattt tgtagttctg
     8941 cttgcaaaat atactcggtt tgatatttga tttacattta ttgaattcac ttatatcgtt
     9001 tttatcaatt ataatcaata tttcaatttc tggagatgtg caaaccttaa taacatcagc
     9061 catatcatta tatggttttt taagtttgaa actttccgat tttgaatcta caaaattcaa
     9121 tattaaaggc ttttccttat atgcatattg tagaaaattt tcagatactt tagatgctgt
     9181 cattcgcttg aaaagcttgt tactatgaag catttctcta gtaaatatga gtttttcatg
     9241 ttcaagaagc aaggtaagaa tatcatactc agcattacct tcacaaagac agattaagtt
     9301 ttctcgattt aaatctagtc ttctctttga catatatatt tcctgacttt ttgtatattt
     9361 tcataattcg gagcagtgcc atctatatag tttgaaagaa ttatgtcact cttctttata
     9421 tcatttctct ttacataatc agaatatttt ataatttcag gagtactatt tactcttctt
     9481 agaataaata tattgtcctt tctttccata acatcaagaa tttcaggata gtgagtagta
     9541 aaaatcaagc atgcaccatt ctgatttatt tcacaatcat taaatagatc tattatcatg
     9601 tgaacaagtt ttttattcat atggttttct atttcgtcaa tgatcatgta cccacctttt
     9661 ttaaaaactt cagctgcttg atttaatata tgcattcctt taatagtacc ggaagataaa
     9721 acttgagatg cgtcaataag attatcatag atgtagtcct ggccattttt aaaaactatt
     9781 cgaacattat tatcctttac agtaattgaa ctgatatttt catcatagag ttttaaaatg
     9841 ttttcgttaa cagtatcatc ccctttaatt aaatagttac tgattgggtg tattgaattg
     9901 taaatatatg gacttgcata tctggttatg gataatacaa tactttggtt atcaggcaaa
     9961 agcgctttcg cttgctcggt caaacctgat cttttcatta taatcatatc atcatcaaac
    10021 tttgtaatat ccgctttttt cttaacactg gtaatcggtt tttcatatat tatctcctct
    10081 ttaaaaaagt aaggataatt ctgttctata tcaatgtatg aatctttttt taatccaata
    10141 atagtatgaa gtctgaatat tttaccagat ataacaaaat caacttttat aactgtatca
    10201 tccttaatta ttccaaatgg tatatttgcc tgctcaagat ttttatgtct agctacaata
    10261 tccataacaa actttataag cctcaacgca gttgttttac ctgaagcatt aatgccaata
    10321 agagaaatta ctttctgtaa atatatacaa tttgatatct tttgtacctg gttatcatca
    10381 acaaccctgt ctgaagcagt catgtcaaaa caaaatcctt tatcaaacac attgatattc
    10441 tcatattcta tccttaatac tttcattatt ctacctcaca aaagcatttt agcataaata
    10501 atacattttg taaacagttt atctgtttat attatttcta ttttaactat ttttaaattt
    10561 tacccttaat ataaacatat aacatgttta taatttcaca tatctacctt aaggtattta
    10621 aatgaaatat tattttttgt ttttctgatt tataattaca aatataaaaa aaggaaagat
    10681 atggctatcc atcagaaaga aaaaactgaa ataacacata atcagcttaa agctactgca
    10741 ttgaagcttt ttcttgacaa gggttataag catgtttacg cattatgaat gattactcac
    10801 attaataaag taacaaccaa atattatcaa catttttaac tatttcgcat tgaacaggat
    10861 gtataaaaaa gatacattaa aatcaaatat gaatctgtat tctagtgaag ttattctgta
    10921 gccgtagtat aattcgtttg tacggacaaa gacatacaaa ggaggacgga atggttacct
    10981 ctagagaaag atttctaaat gtgataaatg gtatcatgcc agacagaata cctgtcaaac
    11041 tgtttatcgc agatcaggga cacttcattt ctcaagtata tcctgatata gatccttggg
    11101 attttccagc tttacagctt aaggttatag aaatacaaaa gcagcttgga ttggatgtgt
    11161 ttcttagaat gctttacgac cttacagacc ctttgcatat ccacatgggc ggcctggaca
    11221 taactcacta aaagtgacaa ctgggagata aaaacagagg attacagaac tggaaatacg
    11281 attgtaaaaa agtcagtaat cagaacacct gatgggactt tagagcagga attctccata
    11341 aatgaaattc gaaaaggaac cttcatgtat gggtgcacta aaaaacctat aaacaatatc
    11401 aaagaactag aaatagcaat taagtatgaa ccaaaaatgc ctgaatactt caaatccgaa
    11461 gctaaaagaa aagtcaaaag aatgaaagat gcagtcggaa acgacggtat tgtcggtgta
    11521 tgggctccgc acggaccatt taataatgct tcattactga ttgatcatga tgtgctttac
    11581 tcattgtatt tagaggacta tgaatattac gaaaaactga tgaactttgc atcagacagg
    11641 atactggaat atgcaactgc aatggatgaa gcaggagcgg acgtgctttg tgtaggcgga
    11701 aatgtagccg gaggtttcgt cggcaaaaaa tattacgacg aatatatcct tccatttgaa
    11761 aaaaagtata tctctttctg ccagaagaac ggtacaccag caatgtatca taactgtgga
    11821 aagataatgg atctggtgga gtcatataag gagcttggcg taaagattgt tgagcctttc
    11881 tcaccgcctc caataggaga tacagatata cttaaagcaa aacagatagt aaacggagac
    11941 tatgtaatgt tagcaggaat agaccaggtt aatgtgcttc aaaaaggtac tatcgatgat
    12001 gtaaaaaggg ctactgcaac actgaccgat atcatgaagc caggtggtaa atgcattata
    12061 cagtctgcag aatctaaggt ctctttagaa atcaccggta atgaagaacg tgatataatt
    12121 cgcaactttt caacacaagc aatagcaggt actgcaagat ttaataggat tgttacagga
    12181 aaaccaacag aacctccgaa tatagtactg cacttcaaag cagaaggtgt gacaccatat
    12241 tctataagat ataatctgac ctgattaata aattgcagta tttgaatatt ttgtgaactt
    12301 ttacgatata atacaattat aaggagtcat caatgagttt attgaaatac ataatgaaac
    12361 agggctggaa ggcaattaaa aaggctgatg cagaccgtat tgcaaagcag ccagaaattc
    12421 taaacactaa ttttgaaagc tgcatcccct atacagatag tattaatcct cttcatcagt
    12481 taaacctgta ttacccattt gagtacgaca tggataaaga cggcatactt ccgacaatta
    12541 ttgatataca tggcggcgga cttatgtatg gcgacaagga actgaatatg agatactgcc
    12601 agtatcttgc atccaagggt ttctgcgtaa tgggaatgag ctaccggttg ttacccgata
    12661 ctgatcttca gggcatgata tctgacattt acacaagcat tcactggctg gagaaaaatg
    12721 gtccaaaaag aggatttgac ttatcaaaag tacttcttac cggtgactca gcaggagggc
    12781 atcttacaag ccttatcatg tgcatacaaa aaagcaagga acttcagaag gtttactctg
    12841 ttgaaccttt taaatcagac atttcagctg tcgcaatcag taacggagtt tgtgaaatgc
    12901 atgactattt cagtttcttc ccttccctta ataagatgat agacagagag attgtcagca
    12961 tgattctggg aaaatccaag aagaatgccc catggaaaga tttcatgagt ttttcgcagg
    13021 tcatggttaa agcagacaaa ttacctgctg tgttagttat tggagctgag aacgacccct
    13081 tctacagaca aaccctgtgg cttatggaga atttaactaa acatggaagc aaatttgaaa
    13141 ctatactttg gaagaaagaa gacgggcctc accttggcca tgttttccac ataggtcatt
    13201 atgaatggca ggaaagtatg ataacaaacg ataagatgct ggagtttttc agaaagaata
    13261 ttttttagat tactcatcta gaaggtgcaa tatgagaaaa atagctgaca atataaaatt
    13321 aagactcgaa tatttaaagg gattcggagc atggacatat cacattagaa ttccaggtac
    13381 ggctaacata gaaggaagat ggggatatat gaaagtgtca gggaccattg acggatatga
    13441 aattaagaac ctcaatcttg ctccaagaaa gaatgaagac aagataatct cgataaataa
    13501 gaagatacgt gacactatag ggaaaactgg tggagatatg gtgaccgtca ctttatatct
    13561 taatcaataa ttactatatt aaaaaatata tctactatgt aaaacaaatc aaaaacctat
    13621 ataaatctgc ttccatattc cattgctgta tcaaaatatg tctttatgtt tatttcagga
    13681 gtcccttctc taaatgagtc agaagaacca agtatgaaac gcccattagg agcacatata
    13741 cttaaggcat caacataaat ggcaggagag taaaataaca aacaataaga tgctggagtt
    13801 atcagtaagt atatagaaaa gacagcacaa atataactat gcagtttata tgttcaaatc
    13861 tacatacatg aatgtagtat tcatttttcc ctttagtttt tcagttctgt tattggtact
    13921 acaaaaacac catcatctct acgatatgca gcatttgaca taccacaaat aacacacaaa
    13981 gaacaaggcg cttttccatc ttcctcctcg attgcctttt ttatactaag aagatttctt
    14041 gctgcatcat caatttgatt tgcacctagt tttatctcaa atgcacacca gtttccatta
    14101 gataattgta ttactgcatc gatttctcta ttttcataat cctgataatg atatagtcta
    14161 gctccaaaag actctgcata aatatttaaa tccctttcgc acattgcctc aaacaaaaaa
    14221 cccaatgttt ccaaatcatt tattaacata tctggagtaa tactaagtaa agcacatgct
    14281 aacgaaggat cagtaaaatg cctcttctgc tgttgtctga ctcttacctt tgatcgtatt
    14341 gaagaagcaa aaggcagctg gttttctata ataaataaac gttcaaatat atttaaataa
    14401 gatgagactg tatttgtatc tatatcctca tcatctattt tcttaatatc tcttatcaaa
    14461 gtattatttg acactgtagt actttcattt ctcgctagcg atctcaataa tagttttatt
    14521 ttgtccaaat ctcttttaat gccatcaact cgatatacat cattgttaat aattgctgat
    14581 aagtatgcct ttggaagtaa cattgcttgc ttaggtaaaa gtttaatact ttctggccat
    14641 cctcctctca aaattaggtc tataatttta cgcaaatcta actgatcagt tttgcatgga
    14701 ttgagtattc ctttacacaa ctgttcaaga gaaatatttc ctaaagaatc accagactca
    14761 tacaaagaca ttggtctcat tcgcaaagaa gcaatcctgc ctgcaccgct atgtaatata
    14821 cctttatgat ttggtgtaga agaacctgtc aaaataaatt gacctttacc tggtctttga
    14881 tcaacaaaat gccttaccgc atcccatatc atcggaactt cctgccattc atctatcatc
    14941 ctaggcgtat caccttctag tactaatgat ggtgcagttt gtgcgagagt cctgttttga
    15001 aaattaccat ttgggtctga taaaaatatt tcacttttac tatgaaaaga cgatgtccat
    15061 gtttttccac accattttgg accttcaata catattgctc caaatacagc taaatattca
    15121 attattgttt tatcgataat tctcggtcta tataattttc tttgcatggt attacctctt
    15181 taataatatt agtataatac attcagtcat atttatcaat atcattcagt catatttgct
    15241 attttcattc agtcgtattt gatattttca ttcagtcaca tttgtatatc tgtatttttc
    15301 aatttgtact cgtcaaatat ggtagacata aaaaaaattt tttaatgata ttttttttcg
    15361 acattaaaca ctatgcttag catacctttt tattcgaaat tgctgaatag atgcgcttat
    15421 gtaaatcact taattcagta atcatttcag gattcccaaa gttattcttt atgatattct
    15481 ctccaaaatc atgcaataat aaggtttgaa atatcttatc tgtcaaatca gaaacatcat
    15541 aatctgcacc gaaaaaacct ttcatatatg gatattcaaa ttcaaatgtt tcacataaaa
    15601 gtgcagctaa ttcatattca caaggtgctt gtacactgtc agcaaaatca attatgtaaa
    15661 tattcatatc tttatcaata agaatattat caggattcaa gtcgccatga acaaatacct
    15721 tatctttcaa atcatacttt ttcaaccaat ccaacctttg cctttgaaaa cttattgaaa
    15781 agctattcca acatttagct tttagcgcac ggtttatgac atcaatagca ttaaattttt
    15841 cacaaggagt attcattctg tcacagataa ctcggagttt tcttccgatc ttttctttct
    15901 gtaagtcatt aaagctctct tcgactttca ccatctcttc agcatctaca tactccatta
    15961 ccatatacat aaattcatac ttgtcgtgga ttacaccgct tcttataagt cttggtactg
    16021 gaatttttaa agattcagtc cttaccagtc cgaatttttc agtggtgtaa tctgaataca
    16081 tatccattcc agattctttt ggagcaaata tcttaacaac ataatctcct gacttgaata
    16141 ctgcattagt acctggcgtc agatgctcaa ttttcgcata aggtaaatct tctattctaa
    16201 aaatatattc aattaacgtt tcccatgcca atatatcctg aaaaacctta ctccaggaaa
    16261 tccagtcgtg tatatcgtgc gtgaaatata tcattttaaa tattctacct ttatgttata
    16321 tgttaatcaa aaacctataa aaatctgctt ccatattcca ttgctgtatc aaaatatgtc
    16381 tttatgttta tttcaggagt cccttctcta aatgagtcag aagaaccaag tatgaaacgc
    16441 ccattaggag cacatatact taaggcatca atcacagtat cccttacatc cttcgctgtg
    16501 cctttttgca tgacgtatat tatatccaca tatcctttca aggtgacttt gtctcctgca
    16561 agtttttttg ccttcaccaa atcgatatcc cctactgtcg gtggagtaag agtttcaata
    16621 acatccggtt tacaggatat ataatcttca agtatatcca tcatttttcc atcatcataa
    16681 aagtcataag tcgcatcata gctatgtata aggtcaatat gctttcgaag tacaggaata
    16741 aatttctgtc tgagaatttc aggagaccat cctgttgaca gtgatgaata ataccaggaa
    16801 acaaaaaagt tgacaacgcc tttttccaga cagaccttag tggtatccat cacatattca
    16861 gtgaagaaat ccattatctt gtcaaacagc tccatattgt cataatagtc gatcatcaaa
    16921 ttttctattc ctcttgcatc acctgccaga tgatcaagag ggcttcttaa gaacaactga
    16981 gttaaaccct catttttcaa tttatcttct gtttcattga aatttacaag attaagatgc
    17041 ttcttcgggt ctggaagaac ataccttagt ttttctatat cagaagcatc ttttattaaa
    17101 aattctcttt ttacaggatt gggagtaacc ccgtatttac cattggaagg cggaacatga
    17161 ataatgtcct taagtttacc tgcgggtgtg gaaatagtac gttcgactat cttcatttca
    17221 ccattagcag tttctttgta atccaccttt atgtcttttg tataggatga atcaaaatat
    17281 acagaatcaa ctggattaac aagccctggc atcatttgat aaactggatc gtgcttgtaa
    17341 atcttgttaa aattaatcac agtttcaaca ccatgtgata aatacctgct cttaagataa
    17401 gcaccgattc ttggagaaca aggaatcctg tctacttctt ttccccttat agcattaatc
    17461 tgcctttgtc tgctggtcat ctcttttttc atatcagact cctatatata catttatatc
    17521 acaaatattg acttgttgct taaaatgttg aaatagtgct tgatataagc ataaagtcaa
    17581 tggataattt atgaaacaat tttaaattcc atacatatat tcaaattaac ggacatatga
    17641 acgaccctca taaatagtaa aatccagttt attctttaca tcttttcagc ctatatccga
    17701 caaaaaaaca tatgatagct tttattaata aaaatatgcc gattagtaat gcgacaactg
    17761 ggataatgaa agcttgtttt ggaaagtcat tcagtggtgc aatattagat aatactgaca
    17821 agtttgcaaa acatacaaat acggcaacaa ctaaaccgat tgcatatttt acagaaatat
    17881 aatatgcgcc gacatatcct ttatcatcaa gtagttgaac cgctttttta ctgagtttga
    17941 atttctcgac tgtatccgaa acatttatcg ttcttgcata ttcctcagca tcgccaaact
    18001 catcttttaa caggaaatct gttgtcacat tgaataatgt acttattttc aatacatttt
    18061 ctatgtcagg aacagaagtg ttcagctccc attttgatag agtctgacga gttatagata
    18121 atttatcagc aagctgctcc tgagaccacc ctttttgttt tctgaacata tatatttttt
    18181 ctccaactgt cataaaacat gcctcgttgt attcaaatac tatcacaagc gatatagcta
    18241 tactaccaat tgaagtttgc atatgaacta cttgggtttg caaaagtcag aataactata
    18301 aaaagagcta atttagatca ataagttttg cttttacacc ttcaagtttg tttaaatcct
    18361 ttacaagctg ttcgacatct tttagttctc cacaaggctg aaggattatt aaaccgtcat
    18421 ttgcgcatac atcctcactt acttcatgca agcctaagcg ggcttttatc ttgcagccat
    18481 tttctgtgag cacttcctgt aacttcaaag atacattgac tctgttgtct atacggacac
    18541 ttattacata ataacaagac attttcttcc tccttgtata ttgtttatgt aatcaggtaa
    18601 atgtcctgat caacactatc t
//
LOCUS       DAOSXM010000038        18100 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2111
            NODE_1972_length_18100_cov_9.947631, whole genome shotgun sequence.
ACCESSION   DAOSXM010000038 DAOSXM010000000
VERSION     DAOSXM010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864178
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 18100)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18100)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:24:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,261
            CDSs (total)                      :: 2,229
            Genes (coding)                    :: 2,218
            CDSs (with protein)               :: 2,218
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 3 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18100
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1972_length_18100_cov_9.947631"
                     /isolate="AMR_MDS_2111"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1403
                     /locus_tag="PLQ68_06285"
     CDS             <1..1403
                     /locus_tag="PLQ68_06285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72295.1"
                     /translation="MIPVLQNRWVIKKGYLQYYGLRSKPYTFNNKITIYKKTEKIIHS
                     FDGIRKINDIKINSQIKKLIRRKVIVDVLEVKKRIESFEQATFCKKCCANDYMIPGLQ
                     FNKSGICPICENMPSLSLIKDVLPIRNIIEEDPNGKYDVAIFYTGGKDSSYLLYYLSI
                     VLNLRVLALTWEIPFMSENAKKSMNNAVSLLKNTDFIKKSLTPKQQSVIYKKAYELQN
                     NTCICPFAAYILFIDDLRKFKVKYLVLGNEPAQPINLIFNNLAPINFFNPIFQKLFRL
                     IYNLTRILKFRKPLKHGQIEFLMLLETLAYGKPETFGRKNTKNPIISNIHKSLSEADD
                     LMKTFSKTIKQCSLDNNLPALVHVDFNDISEGTYKWSDTKKVLKEKIGWQESSFKNKG
                     LHTSCNIESCKEYSQFKAFYDMKSEIIPFSAIELCIAVNMGNISREDAIKEIRENSGF
                     SNIPPYETKQMMESFK"
     gene            complement(1396..2196)
                     /locus_tag="PLQ68_06290"
     CDS             complement(1396..2196)
                     /locus_tag="PLQ68_06290"
                     /inference="COORDINATES: protein motif:HMM:NF038196.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HXK72296.1"
                     /translation="MNCLVCYFSPTKNTENVVRIIIEKAKHTYEDIDVNNTTIIENRY
                     KDIPYVKHYDLLIVSFPVYSQRMPKIFSEYLFSQHINCDKALIVTTYGGVTIGNALED
                     CSAKLLKLGIKTYGAISVPEKHSYASAFEKYEYYNIAQEEKKKILENFFDKCQENLEK
                     NKPISLRRKTDAAVFFSQRFISRLTVCIPKVNLSKCVRCKKCIKSCPTNAIDENLVIN
                     SKECIRCCACVNVCEFDARKCEIKNIISKKIIRTGMNLEKMNDFRIFI"
     gene            complement(2193..3293)
                     /locus_tag="PLQ68_06295"
     CDS             complement(2193..3293)
                     /locus_tag="PLQ68_06295"
                     /inference="COORDINATES: protein motif:HMM:NF025059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HXK72297.1"
                     /translation="MEILKNIKSEDLLMVSFGKSTGEIKKAKIRRYEDIFQLEYIVNN
                     KAFHKNMKSDDVLVQIESLINNDFKECIVYTTKNDINIFRNKKNELKVKYHKPTKSID
                     SKTHDRQKKMIISDGISAPFLYDLGIADYEGNILDKKSRKFVQINRFLQIIDQSFDKI
                     QFNHIPLRAVDYCCGKGYLAFALQYYFEKIKKIQACITGIDLKEDVIENLNQIAKKYE
                     MDNIKFLYKDISDFNEPLDVALGLHACDIATDIFLSSAVRNNAKLIISVPCCQHQLFS
                     QIENEQLKSLLSYGLQKDRFTEMLTNTLRVLALKSRGYSVDMIEFTAFEHTMKNVLIR
                     AVYTNNIDVQAKKEYDKLKAMYNITKFSGDLI"
     gene            3406..3786
                     /locus_tag="PLQ68_06300"
     CDS             3406..3786
                     /locus_tag="PLQ68_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004620367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HXK72298.1"
                     /translation="MKKKIIKVSGCPEAVGPYSQAVRANDLLFVSGNLPIDVETGKIT
                     EGGIELQTHMVLKNISKLLDGAGLQLSDIIKTTIFIKDMNDFAKVNSVYSEYFTSDYP
                     ARSTVEVSRLPKDALIEIECIAKY"
     gene            complement(3783..5270)
                     /locus_tag="PLQ68_06305"
     CDS             complement(3783..5270)
                     /locus_tag="PLQ68_06305"
                     /inference="COORDINATES: protein motif:HMM:NF012697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HXK72299.1"
                     /translation="MNEIGNRKIDITTIPGIRKYRNTLQLILLKVLHDIFPHRKVKIE
                     YSINKGCYCNIDSIRITQDDIDKIKRNIQNYIDSDVEIKEIYCTRSKAIKLLREHNIH
                     DIADSLEYVSNKKVLIYELDGFLSVFYSEVAESTGYVDLFDIKPYDPGFIFFYPTRHK
                     PFELPMYTEQNKLASVFGEFNKWNEILGIPDVTSLNKKVQEGSINDIILASEALQDIK
                     LGEISRDISKNQMKFIMIAGPSSSGKTTFANRLRIHLIVHGINAKIISLDDYYINRSD
                     MIPDEYGKVDLESIDALDIRTFKENMNELSEKGFTKIPSFDFPTATRRPELIPISLKN
                     DEVLIIEGIHGLNPLMTEGIRDEHIFKIYISALTTINIDNHNRFSTTDTRLIRRITRD
                     YQYRNASASHTLHMWESVRSGEDKYIFPFQEQADVIFNSALLYEHGLLRNIAESILRK
                     VKKDDSAYCESRRLLDILSAFERIDSNAVPPSSIIREFIGGSCFH"
     gene            complement(5263..5958)
                     /locus_tag="PLQ68_06310"
     CDS             complement(5263..5958)
                     /locus_tag="PLQ68_06310"
                     /inference="COORDINATES: protein motif:HMM:TIGR01906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HXK72300.1"
                     /translation="MRIAIRIMAIIFAIILCFVFLLSAIRYVAFNQDIYRRIQVKQKI
                     AEFVLMDQNELDNITKGLIDYMKGDRDDLVIYRDSDDQKNEVFNDREKKHMVDVRMLF
                     DLSISVIVVLLSILGLIIISGMLMDMEIMKRIFIKTVAIAMLIFVILMIMLIFYMIVD
                     FQNFWLIFHETVFTNDLYFLDPYTDILINIMPLDFFISICLNISLYFGLLFSGAFAIM
                     YGTKVLKRIVNHE"
     gene            complement(5955..8933)
                     /locus_tag="PLQ68_06315"
     CDS             complement(5955..8933)
                     /locus_tag="PLQ68_06315"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HXK72301.1"
                     /translation="MTNTDFFAAIADTKIKLRKELMESKQPVKAPNIFFKQTENRKNN
                     NKLIFMNPDEEYEESLKQYSKDIENLKNHFKKFLTRKNEPSKERRKKYLIDFDFRYEN
                     AEDLQNFKRILEGKGDWEKIKIPHYHGPTGKWVSYYRTIFDYDKKDNSAFLVFKGSDY
                     ITDVYLNGRHVLNHEGFFYSFEQDITQYLLEKDNVLVVKVENDITTLGENRKYGKKIY
                     AATGIGWDDPLDGWHHCPPGGGIFDKVYIEQREVIHTVDACVFPDIDNNLAKVNVIVN
                     SLSLENTKADVALTVEPFNFDDSLKLTQSHEIILNHGENNYSYDIAIDNPRIWTNDTP
                     YLYTLKIKIDKKDEYELNFGMRKFHMDTVTKPYGTLYLNNSEIILRGANEMGHLQLCV
                     MNEDYDQLIEDILIAKYCNMNYYRITQRPVQREIYEYCDMLGMMVQVDFPLFGQLIKS
                     QIYEASRQIGEMERHIRRHPSVIMVSYINEPTDQYKSQTAHINLDREEMENWFDICDR
                     YIRYENPQRVIKRVEGDYDPPTFEGLSDFHCYNMWYTNHALPIGDLYKGYLPATRKDW
                     KMGCGEYGTEGLDNLSLMKKYYPKQWLPANDDDFWIPDKIIKAQTNSMHGDWFYEQDN
                     INDWIKFSQNHQSKSVNLMTLALRRRSDYIVQTALHLLIDAYPSGWMKTVVGVDRVPK
                     PAYFEFKKALKPLKLNFRSDRWSCYTEDFIDVESWILNDTNSEYKDIVIKANLYDSKG
                     YIHKSYTLNTEISECISKPVGQIRIDTSDIDINSREKMVLKGELIINGEVTDTEVFIF
                     KLFERLKDRIYAYPIGDIATEVVNTSDAFNNSDIDSCQIVIISDFSKSKELISGRLLA
                     NKPVLYILNEDNPQIIIEKRQYTISDKRMWKATYSPINQQLLTHFEWDDISFLYDSKL
                     DKINFSAEYFIECDEVDDIYIFNYKKPSFSESTEGKKEKRPVLMKNMDGIFTTLNLEG
                     RTGVNPAIDYILYDVIRRIL"
     gene            complement(8944..>9849)
                     /locus_tag="PLQ68_06320"
     CDS             complement(8944..>9849)
                     /locus_tag="PLQ68_06320"
                     /inference="COORDINATES: protein motif:HMM:NF024572.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXK72302.1"
                     /translation="LPSRNKKMTPLAFIKIQMVIIIALLVVILVLIAVMIGMKKKFDN
                     YVSVQPDLMAKIETLEQEQEEQEALYQSTVTQYNTTIADLENRLSSSDTDIEAAQKES
                     EMLTQKNYLYYGKYLASDLKYNDALSTLLQIELEKAFFTESELKLYDETLELSRMNIA
                     ISLYNSGNKLYQDLKYKEAFSNFYEIWNYYPDYQLVAKWEGNTVYSNLVYDSVYKMSR
                     CAYEIGEFNTAISGYEFIEKSNSEFANANIEGLIYHSAKAYAGIEDYVKAEQLFQKVI
                     NEYPNSELVTYAKERLSAVQDKLGR"
     assembly_gap    9850..9949
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(9997..11280)
                     /locus_tag="PLQ68_06325"
     CDS             complement(9997..11280)
                     /locus_tag="PLQ68_06325"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HXK72303.1"
                     /translation="MNYNDAVAYIHSASKFGSKLGLANITKLLELLGNPQKELKFIHV
                     AGTNGKGSTSCFIANILYSSGYKTGLFISPYVESFGERMQVNFKNIDDDSLIQNVEMV
                     KETIDVMLEQGFNHPTEFEIDTAIAMLYFQQSKCDVVVLEVGLGGRLDSTNVIESSLS
                     TVICTIEKDHMSILGDTLEKIAYEKAGIIKKGCSVVVYGSNDACTYEVIKTQCKIMSA
                     PYQIVDFNQISDIRYYDYSYTFNFESFKDLKINMFGDHQIKNACTAIKAVQAATSLEI
                     SEASIREGLKNTKWPGRLEILSRNPLVICDGAHNPEGIRSLKNTVVKYFPDKKKIFVM
                     AVMKNKEYEKMIEDIFPIADVAIAVRPSYDRALELDILKETIQKYCKNTKAFDKIEDG
                     LDYAVENAVENSVICTFGSLYYIADVKNYIRKTGL"
     gene            complement(11284..14055)
                     /gene="secA"
                     /locus_tag="PLQ68_06330"
     CDS             complement(11284..14055)
                     /gene="secA"
                     /locus_tag="PLQ68_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014062391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HXK72304.1"
                     /translation="MGKLLDRIFGTHSDQEIKKIIPIVDLIESMSDEMKQKTDSELKE
                     YTNIFKSRLEEGQTLDDILPEAFAVCREASTRVLNMTPYRVQLIGGIVLHQGRIAEMK
                     TGEGKTLVATLPIYLNALSGKGVHLVTVNDYLAKRDSEWMGKLYNFLGLSVGLIGYGM
                     SKEEKQAAYNADITYCTNNELGFDYLRDNMVTRKEDLVQRDLHFAIVDEVDSILVDEA
                     RTPLIISGAGEQSSDLYKKADVFAKTLKKKVVIEEESSGKLQRIMDMNSSEEDDEYAE
                     DKPDYIVDEKNRTASLTPKGVERAEKYFGVESLTSYENTTLLHHINNAIKAHSIMHKD
                     IDYVIKDNQIIIIDEYTGRMMFGRRYSEGLHQAIEAKENVKVERENKTLATITFQNFF
                     RMYHKLSGMTGTALTEEEEFREIYKLDVIAIPTNKPVIRVDHPDVVYKTRKAKLEAIV
                     ADIIECSKTLQPVLIGTISIEKSEELSLMLRKRGIKHEVLNAKHHEREAEIVAQAGKS
                     GSITIATNMAGRGTDIVLGGNAGYMAMQKLRKEIFDEDILIQADSYNETDNEDILHAR
                     KLYREYEAQFKQMIKDDRDIVLEKGGLYILATERHESRRIDNQLRGRSGRQGDPGMSR
                     FYLSLEDDLMRLFAQDRLSNMMDLLKVPDDIPIEAKIITNTIETAQKRIESINFQRRK
                     TVIQYDDVMNTQRNLIYGQRRQVLDGTDMKNSYLKMLEAVITRLTDDYCSHQRSQEWN
                     VEAIRERLNDLLPAEYNPITSDEDDLSEYNMKSFNQMLLDNYRSKYEKKEQELGEQLM
                     REIERVVLLRSVDMHWMDHIDAMDQLRSGIGLRAFGQKDPVMEYKFEGYTMFEEMTNS
                     IQQEALRRMFSIHKQTEVQKEESKIKINAQLAPDDIQKPKTSANSKVGRNDPCPCGSG
                     KKYKKCCGA"
     gene            complement(14048..14344)
                     /locus_tag="PLQ68_06335"
     CDS             complement(14048..14344)
                     /locus_tag="PLQ68_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014312859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YerC/YecD family TrpR-related protein"
                     /protein_id="HXK72305.1"
                     /translation="MNEKLKSKESDYLFDAILTLKSREECYSFFEDICTVAELKAMAQ
                     RIVVAKMLNEKHTYTDISLKTGASAATISRVAKSLYYGAEGYTTVLKRLGDING"
     gene            complement(14349..15326)
                     /gene="galE"
                     /locus_tag="PLQ68_06340"
     CDS             complement(14349..15326)
                     /gene="galE"
                     /locus_tag="PLQ68_06340"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HXK72306.1"
                     /translation="MSILVTGGAGYIGSHMVLHLLEKGYDVVIVDDLSTGNKEAVLAN
                     RFYNADLKDSKAMDKVFRENNIDGIIHFAAYSLVGESMQNPIKYYENNVFGTLKILQY
                     MENYGVKNIVFSSTAAVYGEQPSPIKESARTNPTNPYGETKLSIEKMLKWASKAYGIN
                     YAALRYFNAAGSHESGRIGEKRKIETHLIPIALDVALGKIDKLLIFGDDYDTPDGTCI
                     RDYIHVCDLVSAHLLALEKMQNSNNCALSMTYNLGSEKGFSVHQIVKAVKDVTKKDIK
                     TEIAPRRKGDPAVLVASSEKIKKDLSWNPRYNDIHDIISTAWEYRKKLR"
     gene            complement(15327..>16247)
                     /locus_tag="PLQ68_06345"
     CDS             complement(15327..>16247)
                     /locus_tag="PLQ68_06345"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF003705.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HXK72307.1"
                     /translation="ELEKYKNLKWGNYQLGVIEQLMKKGISVGGAEFIFNDLVPLGSG
                     LSSSAAIEVVTAYAMTELYGHNIDKIEIALICQKAEHEYVGVKCGIMDQFASAMGKKG
                     NAILLDTDTLEYEYIPVKLDKYSIVISNTNKKRSLGESKYNERRSECEKALADMQNKF
                     PEKKNLCDFSYKEFLLCKDEIKDEKCKKRAEHAVCENLRVLDSVKSLRKNDILSFCKY
                     LNLSHISLRDLYEVSCKELDILVESAWMQKDVLGSRMTGAGFGGCTVSIVKTESVEEF
                     INDISKVYYEIIGYDASFYVCNISDGVKEI"
     assembly_gap    16248..16347
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<16348..17123)
                     /gene="galT"
                     /locus_tag="PLQ68_06350"
     CDS             complement(<16348..17123)
                     /gene="galT"
                     /locus_tag="PLQ68_06350"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006873026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HXK72308.1"
                     /translation="MAELRWHPLIKDWVMVVSQRQNRPQMPKDWCPFCPGSGKVPDDF
                     IVYKYDNDFPSLSLNPPNPDDVETDLYKVDKAYGKCEVILYSKDHNAKLCELDDLHLN
                     ELVNLWQDRFKDISTYEKIKYVFIFENRGEAVGVTMPHPHGQIYGYPFIPKKIQLELA
                     SCKEHTEKTGRCLICDMNKNEKDFDKRVIFENDSFIVYLPFFSEYPYGIYVSSKRHIQ
                     YITEFTEKEKSDFAKTLKQAVGTLDNLFGFDFPYMMCMHQA"
     gene            complement(17127..18029)
                     /locus_tag="PLQ68_06355"
     CDS             complement(17127..18029)
                     /locus_tag="PLQ68_06355"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015357985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HXK72309.1"
                     /translation="MGRKDNKLGDWINSELPKIASDLESLNDDYYLRENTISLAGKER
                     VKNILFLLRSALFPGVYEKCSISKANVDMLIGNNIREAAIELREVIDRVFDYKCKKDN
                     TECEIDPDSVIISFLEKIPEIRRMLQTDIHAAYVGDPAAKSFEEILLSYPSLEMMSIH
                     RIAHELYRLNVPLIPRMMNEYGHRITGIDIHPGACIGEHFFMDHGTGIVIGETCRIGK
                     NVKIYQGVTLGAKSFELDADGNPVKGIKRHPDIEDNVIIYSHATILGGDTVIGHDSVI
                     GASVWLMKSVPPHSTIYHNLRSEK"
BASE COUNT         5629 a         3300 c         2329 g         6642 t
ORIGIN      
        1 ttatgattcc tgttttacaa aatagatggg taataaaaaa gggttattta cagtattatg
       61 gattgagatc aaaaccatat acttttaata acaagattac tatttataaa aaaacagaaa
      121 aaataatcca ttctttcgat ggtattagaa aaataaatga tatcaagata aattcccaga
      181 ttaaaaaact tatacgcaga aaagtaattg tggatgtcct agaagttaaa aaaagaattg
      241 aaagttttga gcaggcaaca ttctgtaaaa aatgttgtgc aaatgattac atgattccag
      301 gactacagtt caataagagt ggcatttgcc ctatatgtga aaatatgcca tcattatcct
      361 taataaagga cgtgcttcca atcaggaaca ttatagaaga agatccaaat gggaaatatg
      421 atgtagcaat cttttataca ggaggaaagg attcctctta tttattatat tacctttcca
      481 ttgtcttaaa tttaagagtt ctggcactaa catgggaaat tcctttcatg tcagaaaatg
      541 caaaaaaaag tatgaataac gcagtatcgc ttttaaagaa tactgacttt ataaaaaaat
      601 cactgacccc aaaacaacaa tcagttatct ataaaaaagc ttatgaatta caaaataata
      661 catgcatatg cccatttgca gcatatatat tatttataga cgacttaaga aaatttaaag
      721 taaagtacct tgttcttggc aacgagcctg ctcaaccaat taatctgatt tttaataatc
      781 ttgctccaat aaacttcttt aatcctattt ttcaaaagtt atttcgtctt atttacaatt
      841 tgacacgtat actcaaattt agaaaacctc ttaaacatgg ccagatagag tttctaatgc
      901 ttcttgaaac attagcatat ggtaaacctg aaacattcgg aaggaaaaat acaaaaaatc
      961 caataatatc aaacatacat aaatctctat ctgaagctga tgatttaatg aaaacttttt
     1021 caaaaacaat taaacaatgt tccttggata acaatttacc tgctttagta catgtagatt
     1081 tcaatgatat ttcagagggc acatataaat ggagtgatac gaaaaaagtt ttaaaagaga
     1141 aaatcggctg gcaagaatca tcatttaaaa acaaaggact tcatacaagc tgtaacattg
     1201 aaagctgcaa agaatattcc caattcaaag ctttttatga tatgaaatct gagataattc
     1261 ccttttctgc aattgagctt tgtattgcag tgaatatggg taatatttct agagaagatg
     1321 caataaagga aatcagggaa aactcaggtt tttctaatat tccaccttat gaaacaaaac
     1381 aaatgatgga atcatttaaa taaaaatcct gaaatcattc atcttttcca aattcattcc
     1441 tgttcttatt atcttttttg atataatatt tttaatttca catttccttg catcaaattc
     1501 acatacatta acacaggcac agcatcttat gcattcttta ctatttataa ctaaattttc
     1561 atctattgca tttgtaggac atgattttat acatttttta catcttacac atttacttaa
     1621 attcactttt ggaatacata cagttaatct gctgatgaat ctttgcgaga aaaacacagc
     1681 tgcatcagtt tttcttctta atgatatagg tttatttttt tctaaattct cctgacactt
     1741 gtcaaaaaaa ttttctaaaa tctttttctt ttcctcttga gcaatattat aatactcata
     1801 tttttcaaat gctgatgcat atgaatgttt ttctggaaca gatatggctc catatgtttt
     1861 aatacccaat tttaacaatt tagcggagca atcttctaat gcgttaccaa ttgtgacccc
     1921 accatatgtt gtaacaatta acgctttgtc acagttaata tgctgtgaga ataaatattc
     1981 tgagaaaatt tttggcattc tttgtgaata aactggaaat gacacaatca acaaatcata
     2041 atgttttaca tatggtatat ctttatatct attttcaatt atagtagtat tgtttacatc
     2101 aatgtcttca tatgtgtgtt ttgctttttc tataataatt ctaactacat tttcagtatt
     2161 ttttgtaggt gagaagtaac atacaagaca attcatatta aatccccact gaattttgtt
     2221 atattataca ttgctttaag tttatcgtat tctttttttg cttgtacatc tatattattt
     2281 gtatatacag ctcttataag gacattcttc atagtatgtt caaatgctgt aaattcaatc
     2341 atatctacac tgtatcctct tgattttaat gcaagaactc ttaatgtgtt tgttagcatc
     2401 tcagtaaatc tgtctttttg taatccatat gacagcagag atttcagctg ttcattctca
     2461 atctgactaa acagctggtg ctgacagcaa ggtactgata taattagttt tgcgttattt
     2521 cttactgcac tggataagaa aatatctgta gcaatatcgc aagcatgaag gcctagtgct
     2581 acatctaacg gttcattgaa atcagatata tctttataga ggaatttaat attatccatt
     2641 tcatactttt tagctatctg atttaaattt tctattacat cctcttttaa atcaatacct
     2701 gtaatacaag cctgtatttt ctttattttt tcgaaataat attgcaaagc aaaagcaagg
     2761 tatccttttc cacagcaata atcaacagct cttaatggaa tatggttaaa ttgaatttta
     2821 tcaaatgact ggtcaataat ttgtaaaaat ctattgatct gaacaaattt tctgcttttc
     2881 ttatctaaaa tatttccttc ataatctgca ataccaagat catacagaaa tggagctgaa
     2941 attccatcgc taattatcat tttcttttgt ctgtcatggg ttttgctgtc tattgattta
     3001 gttggtttat ggtacttaac ctttaattcg ttttttttat tacgaaaaat attaatatcg
     3061 ttttttgtag tatatactat acattcttta aaatcattat ttatcaaaga ctctatttga
     3121 actaatacat catcagattt catgttttta tgaaaagcct tattattaac tatgtattcg
     3181 agttgaaaaa tatcctcgta tcttcttatc ttggcttttt taatttctcc tgtggattta
     3241 ccaaaagaaa ccattaacaa atcttcactt tttatatttt ttaaaatttc catgtttcaa
     3301 ttatatcatt aaaatcatac atacaatttc ttttattaat attccattag tgataatata
     3361 ttaaagacac ctttaaggtg aaattgataa aaaggatgta ttattatgaa aaaaaagatt
     3421 ataaaagtat cgggttgtcc agaagcagta ggtccatatt cacaagcagt aagagcaaat
     3481 gatcttctat ttgtatctgg aaatctgcca atcgatgtcg aaactggcaa aattactgaa
     3541 ggagggattg aacttcagac acatatggtt ctaaaaaata tatcaaaact tttagatggt
     3601 gcaggattac agctttctga tattataaaa actacaatat ttattaaaga tatgaatgat
     3661 tttgcaaaag taaactcagt atattcagag tatttcactt cagattaccc tgctagatca
     3721 actgttgaag tgtccagatt gccaaaagat gctcttattg aaatagagtg tattgcaaaa
     3781 tattaatgaa aacaggaacc gccaataaat tcccttatta tggaacttgg cggtactgca
     3841 tttgaatcta tcctctcaaa tgctgataaa atatctagaa gtcttcttga ttcacagtaa
     3901 gctgaatcat ctttttttac tttacgcaaa attgattctg caatatttct taataatccg
     3961 tgttcatata ataatgcact gttaaaaatt acatcagctt gttcttgaaa tgggaatatg
     4021 tatttatctt ctccagagcg taccgattcc cacatatgaa gagtatgtga tgcacttgca
     4081 ttgcgatatt gataatctct tgtaattctt ctaattaatc tggtatctgt tgtagagaat
     4141 ctattatggt tatcaatatt tattgttgtc agagcactta tgtatatctt aaaaatatgc
     4201 tcatctctta ttccttcagt catcaaaggg tttaatccat gaataccttc tataatcaat
     4261 acctcatcat ttttcagact tatgggtata agttcaggtc ttcgcgttgc agtaggaaaa
     4321 tcaaaagatg gtattttagt aaatcctttt tctgataact cattcatatt ctctttaaat
     4381 gttctgatat ctaaggcatc tatactttca aggtctactt taccatattc atctggaatc
     4441 atatccgatc tgtttatata gtaatcgtct agcgaaataa tttttgcatt tattccatga
     4501 acaattaaat gaatccttaa tctattagca aatgtagtct tgccactgct tgaagggcct
     4561 gcaatcatta taaatttcat ttggtttttg ctaatatcgc gcgaaatttc accaagtttt
     4621 atatcctgaa gagcttcact agccagaata atgtcattta ttgaaccttc ttgtactttc
     4681 ttattaaggc ttgtaacatc aggtatacct aaaatttcat tccatttatt aaattctcca
     4741 aaaactgaag ccaatttatt ctgctcagta tacatgggaa gttcaaatgg tttatgtctt
     4801 gtcggataga aaaaaataaa accaggatca taaggtttta tatcaaacag atcaacatat
     4861 cctgtacttt cggcaacttc tgaataaaaa acgcttaaaa atccgtctag ctcatatatc
     4921 aaaaccttct tgttgcttac atattccaat gagtcagcaa tatcatgaat attatgctct
     4981 ctaagaagtt taatagcctt tgatctggta caatatattt ctttaatttc aacatcacta
     5041 tctatataat tttgtatgtt tcttttaatc ttatcaatat catcctgagt tattcgtata
     5101 ctatctatat tacagtaaca ccctttattt atggagtatt caattttaac ttttctatgt
     5161 ggaaaaatat catggagcac cttaagcaat attagctgta atgtattgcg atattttctt
     5221 ataccaggta ttgttgttat atcaattttt ctatttccta tttcattcat ggtttacaat
     5281 cctttttaat actttggtac catacataat agcaaaagct ccactaaata acaaaccaaa
     5341 ataaagacta atatttaaac atatagatat aaagaaatcc aaaggcataa tattaatcaa
     5401 tatatcagta taaggatcta aaaagtacaa atcatttgta aaaacagttt cgtggaagat
     5461 aagccagaaa ttttgaaaat caactatcat atagaaaatc aacataatca ttaaaatcac
     5521 gaatattaac attgcaatag ccactgtttt aatgaaaatc cttttcatta tttccatgtc
     5581 catcaacata ccagaaataa ttatcaaacc taatatactt agtaaaacaa caataactga
     5641 tattgataaa tcaaaaagca ttctaacatc aaccatgtgt tttttttctc ggtcgttaaa
     5701 aacctcattt ttctgatcat cagagtcacg atagataact aaatcatccc tgtcaccttt
     5761 catataatca attaaacctt ttgttatatt atctaactca ttttgatcca ttaatacaaa
     5821 ttctgcaatt ttttgcttaa cttgaattct tctgtaaatg tcttgattga aagctacata
     5881 tctaattgct gataacaaaa aaacaaaaca taatataatt gcaaatatta tggccattat
     5941 tctaatagca attctcataa aattctcctt attacatcat atagaatata atcaatagca
     6001 ggattaaccc cagttctacc ttcaaggttt aatgtagtaa aaattccatc catgttcttc
     6061 attaaaacag gtcgtttctc tttcttccct tcagtacttt ctgaaaatga tggttttttg
     6121 taattgaaaa tataaatatc atcaacctca tcacattcaa taaaatattc tgctgaaaaa
     6181 ttaattttat ctagtttaga atcatataaa aaggagatat catcccattc aaaatgcgtt
     6241 agcaattgct ggtttatagg agaatatgta gctttccaca ttctcttatc acttattgta
     6301 tattgtcttt tctcaatgat aatttgtgga ttatcttcat ttaggatata caaaaccggt
     6361 ttgtttgcta ataatctacc actaattaat tctttagact tagaaaaatc agatatgata
     6421 actatctgac atgaatctat gtctgaattg ttaaatgcat cacttgtatt tacaacttca
     6481 gttgctatat caccaatggg gtatgcataa attctatctt tcaacctttc aaatagttta
     6541 aatatgaata cttctgtatc agtaacttcc ccatttataa taagttcacc tttaagtacc
     6601 attttttccc tggaattaat atcaatatct gatgtgtcaa ttcttatctg accaacaggt
     6661 tttgatatgc attctgatat ttctgtattt aatgtgtatg acttatgtat ataaccttta
     6721 ctgtcataaa ggtttgcttt tattacgatg tctttatatt cactatttgt atcattaaga
     6781 atccagcttt ctacgtcaat aaaatcctct gtataacacg accatctgtc tgatctaaaa
     6841 ttcaatttta aaggttttaa agctttctta aattcaaaat aagctggttt tggtaccctg
     6901 tcaactccca caacagtttt catccatcca gaaggataag catcaattag aagatgcaaa
     6961 gcagtctgaa ctatatagtc acttcttctt ctcagagcca gtgtcattag gtttactgat
     7021 ttagattgat gattctgact gaatttaatc cagtcattta tattatcctg ctcgtaaaac
     7081 caatcaccat gcattgagtt tgtctgagct ttaataattt tatccggtat ccaaaaatca
     7141 tcatcattag caggaagcca ttgttttggg taatattttt tcatcaatga caaattatca
     7201 agcccttctg tgccatattc tccacaaccc atcttccagt cttttcttgt tgcaggcaga
     7261 taacctttat ataaatcacc tataggcaaa gcatgatttg tataccacat gttataacag
     7321 tgaaaatcag ataaaccttc aaatgtcgga ggatcataat ccccttctac tctttttatg
     7381 acgcgttgag gattttcata tcttatatat ctgtcacaga tatcaaacca gttctccatc
     7441 tcttcacgat caagatttat atgtgcagtc tggcttttgt actgatcggt aggttcgtta
     7501 atgtatgaga ccattataac acttggatgt cttcttatat gcctctccat ttcaccaatt
     7561 tgtctcgaag cttcatatat ctgacttttg attaattgtc caaacagagg aaaatcaacc
     7621 tgaaccatca tacctaacat atcacagtac tcatatatct cacgttgtac aggcctttgt
     7681 gtaatcctgt aataattcat attacagtac tttgcaataa gtatatcttc gattaactga
     7741 tcataatcct cattcatgac acaaagttga agatgcccca tttcattagc gcctctaagt
     7801 attatttctg aattatttaa atagagagtt ccatatggtt ttgtaactgt atccatatga
     7861 aattttctca taccgaaatt tagttcatac tcatctttct tatcaatttt tatcttcaaa
     7921 gtatataagt aaggtgtgtc atttgtccag attctcgggt tatcaatagc aatatcgtat
     7981 gagtaattat tttcaccatg attaagaatt atttcatgag attgtgttaa ttttaatgaa
     8041 tcatcaaaat taaaaggttc aacagtaagt gctacatctg cctttgtatt ttctaatgac
     8101 aggctattta caattacatt aacttttgca agattattat ctatatcagg aaagacgcat
     8161 gcatctactg tatgaataac ttcacgctgt tcaatatata ctttgtcaaa tatgccacct
     8221 cccggaggac agtgatgcca gccatctaaa ggatcatccc atccaatacc agttgctgca
     8281 tatatttttt tcccatactt tctattctct ccaagagtag taatatcatt ttcaactttt
     8341 acaacaagaa cattatcttt ttcaagaaga tactgagtta tatcttgttc aaatgaatag
     8401 aagaatcctt cgtgattcaa cacatgtctt ccatttaaat aaacatctgt tatataatca
     8461 gaacctttaa atactaaaaa agcagagttg tcctttttat cataatcaaa aatagtccta
     8521 tagtaggaaa cccattttcc tgtaggacca tgataatgtg gaattttaat tttttcccag
     8581 tcaccttttc cttcaagaat tcttttaaaa ttctgtaaat cttcagcatt ctcatatctg
     8641 aaatcaaaat caataagata tttctttctt ctttctttag atggttcatt tttcctagta
     8701 aggaatttct tgaaatgatt tttaagattt tcaatatctt tagaatactg ttttaatgat
     8761 tcttcgtatt cttcatccgg attcatgaaa attaatttat tattattttt cctattttca
     8821 gtttgcttga aaaaaatatt cggagcctta acaggctgtt tgctttccat caattctttt
     8881 ctgagtttta ttttagtatc agctattgct gcaaaaaaat cagtatttgt caaaattaat
     8941 tccttacctt cctaatttat cttgaactgc agataatctt tctttagcgt atgtaactaa
     9001 ttctgaattg ggatattcat taataacctt ttgaaataac tgctcagctt ttacataatc
     9061 ctcaattcct gcatatgctt tagcgctgtg atatataagc ccttcaatat ttgcattagc
     9121 aaattctgaa ttactcttct caataaattc gtatccgctt attgcagtat tgaactcacc
     9181 gatttcatat gcgcatcttg acattttata gactgaatca tacacaagat ttgaatatac
     9241 ggtattgcct tcccattttg ctaccaattg ataatcagga taataattcc atatttcata
     9301 aaaattacta aatgcttctt tgtatttaag atcctgatat agtttattac ctgaattata
     9361 taatgatatc gcaatgttca tcctggaaag ctcaagagtt tcatcgtata atttcagttc
     9421 agattcagta aaaaatgctt tttccaattc aatttgtaac aatgtcgata aagcatcgtt
     9481 gtattttaaa tctgatgcta gatatttgcc ataataaaga tagttcttct gagtaagcat
     9541 ttcagattct ttttgtgcag cttcaatatc agtatcactc gaagaaagtc tgttttccaa
     9601 atctgcaatt gtggtattgt attgtgtaac tgtagattga tataaagctt cctgctcttc
     9661 ttgttcctgt tccaatgttt cgattttagc cattaaatca ggttgaacag acacatagtt
     9721 gtcaaacttc tttttcatac caatcataac tgcaattaaa actaatataa caactaataa
     9781 agcgataatg attaccatct gaattttaat aaaagccaga ggtgtcattt ttttatttct
     9841 tgacggtaan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc tgaaatcaat
     9961 tttttcaaga tacttctgta gttcattaat atccatttat agccccgttt ttctaatgta
    10021 gttctttaca tccgcgatgt aatataacga gccgaatgtg catataacag aattttcgac
    10081 tgcattttcg actgcataat ctaatccgtc ttctatctta tcaaaagctt tggtattttt
    10141 acagtatttt tgtattgttt ctttcaatat atctaattcc agagctctat cataagaagg
    10201 cctaactgca atagcaacat ctgctatagg aaaaatgtct tcaatcattt tttcgtattc
    10261 tttatttttc attactgcca taacgaatat ctttttctta tcaggaaaat atttaacaac
    10321 agtatttttc aaacttctta taccttcggg attatgtgct ccatcacata taacaagagg
    10381 atttcttgac aaaatttcaa gtcttcctgg ccacttagtg ttcttcaaac cttctctgat
    10441 tgatgcttca gaaatctcta gacttgttgc tgcttgaacc gctttaattg ccgtacatgc
    10501 atttttaatc tgatgatctc caaacatatt tatttttaag tctttaaaag attcgaaatt
    10561 aaatgtgtat gaatagtcat aataccttat atcactaatt tgattaaaat ccactatttg
    10621 atatggagca gacattattt tacattgagt tttaataacc tcatatgtgc atgcatcatt
    10681 agatccataa acaactaccg aacatccttt ttttataatc cctgctttct catatgcaat
    10741 tttctccaaa gtatctccca gtatagacat atgatctttt tctattgtgc atataactgt
    10801 tgacaaagat gattcaataa catttgtaga atcaagccgc ccaccaagac cgacttcaag
    10861 aaccacaaca tcacatttgc tttgctggaa atacaacata gctatagcag tatctatctc
    10921 aaattcagta ggatgattaa atccctgttc cagcataaca tcaatcgttt cttttaccat
    10981 ttctacattt tgtattaaac tgtcatcatc aatattctta aaattcacct gcattctttc
    11041 accaaaagat tctacatatg gtgaaatgaa taatcctgtt ttgtacccag atgaatacag
    11101 tatattcgct ataaaacaac ttgtagaccc tttaccattt gtacctgcaa catggataaa
    11161 tttaagttct ttttgtggat tacctagtag ttccaaaagt tttgtaatat tggccaatcc
    11221 taactttgaa ccaaatttcg aagcagaatg aatgtacgct acagcatcat tataattcat
    11281 acactatgct ccacaacatt tcttgtattt tttaccgctt ccacatgggc aagggtcatt
    11341 tcttccaact tttgaatttg ctgatgtttt tggtttttga atatcatcag gtgcaagctg
    11401 tgcatttatt tttattttgc tttcttcttt ctgcacttca gtctgtttgt gtattgaaaa
    11461 cattcttctc aaagcttcct gctgtatact gtttgtcatt tcctcaaaca tagtataacc
    11521 ttcaaacttg tattccataa cagggtcctt ctgtccaaat gctcttaatc ctataccaga
    11581 acgcaattgg tccattgcat caatgtgatc catccagtgc atatccacag aacgaagaag
    11641 cactactctt tctatttctc tcattagctg ttcgccaagt tcctgttcct tcttttcata
    11701 tttacttcta taattatcaa gcaacatctg attaaatgat ttcatattgt attccgaaag
    11761 atcgtcttca tcagatgtaa tcggattata ttcggcagga agtaaatcat ttagtctttc
    11821 tcttattgct tcaacattcc attcttgtga tctttgatga gaacaataat catcagttaa
    11881 tctcgtaata actgcttcca gcattttaag ataactgttt ttcatatctg ttccatctag
    11941 aacctgtctt ctttgtccat aaatgagatt tctctgagta ttcattacat catcatactg
    12001 tataaccgtt ttacgtcttt ggaaatttat actctcaatt cttttttgtg cagtttctat
    12061 cgtattagtg attatttttg cttctatagg aatatcatca ggtaccttaa ggagatccat
    12121 catattggac agtctgtctt gagcgaataa acgcataagg tcatcttcaa gtgacagata
    12181 gaatctgctc atccctgggt ctccctgtct tccggaccgg cctcttagct gattatcaat
    12241 tcttcgagat tcgtgtcttt ctgtagctaa gatatataag ccaccttttt caagcactat
    12301 gtctctgtca tcctttatca tttgtttgaa ctgagcttca tattctcgat aaagttttct
    12361 tgcatgaaga atatcttcgt tatctgtttc attatagcta tcggcttgaa tgagaatgtc
    12421 ttcatcaaat atctctttac gaagcttctg catagccata tatcctgcgt taccacccag
    12481 aactatatct gtacctctac cagccatgtt tgtagcaatt gttatagaac cggattttcc
    12541 tgcctgagct actatctccg cttctctttc atggtgctta gcatttagta cttcgtgttt
    12601 gatgcctctt tttcttaaca tcaaactaag ctcttcagat ttctcaatag atatagtacc
    12661 tatcaaaact ggctgaagcg ttttagaaca ttcgattata tcggccacta tagcctctag
    12721 ttttgctttt ctggtcttat atacaacatc tggatgatct acccttatta caggcttatt
    12781 ggtcggaatg gctattacat caagtttata tatctctctg aattcttctt cttcggttaa
    12841 agctgtacca gtcataccag acaatttatg atacatccta aagaaattct ggaatgtgat
    12901 agtagctaat gtcttatttt ctctttctac ttttacattt tcttttgctt caatagcttg
    12961 atgaagacct tcagagtatc ttcttccaaa catcattctt cctgtatact catcaattat
    13021 tattatctga ttatctttaa taacataatc aatatcctta tgcattatac tgtgagcttt
    13081 aatagcatta ttaatatgat gaagaagtgt tgtgttctca tatgatgtaa gactttctac
    13141 accaaaatat ttttctgctc tttctactcc ttttggtgtt aatgaagctg ttctgttttt
    13201 ttcatcaaca atataatctg gtttatcttc agcatactca tcatcctctt cagatgagtt
    13261 catatccatt attctttgaa gcttaccgct gctttcttct tcaattacaa ctttcttttt
    13321 aagagtttta gcaaatacat cagctttttt atataaatct gatgattgtt caccagctcc
    13381 agatataatt aatggtgttc ttgcttcatc cactaatata ctgtctactt catcaacaat
    13441 agcaaaatgt aaatctcttt gtactaaatc ctcttttcta gtaaccatat tgtcacgaag
    13501 ataatcaaat cccaattcat tattagtaca gtatgttata tcagcattat atgcagcctg
    13561 tttttcttct ttagacatcc catatccaat tagaccaaca gaaagaccta agaaattata
    13621 taattttccc atccattcgc tatctctttt tgcaagataa tcatttacag taacaaggtg
    13681 aacacctttt ccagaaagag cattaagata aataggtaaa gttgcaacta aagtttttcc
    13741 ttcaccagtt ttcatttctg caattcttcc ctgatgaagc actattcctc caataagctg
    13801 aactctgtag ggagtcatgt tcaatactct tgttgaagct tctctgcata ctgcaaaagc
    13861 ttcgggaaga atatcatcca acgtctgccc ttcttctagt cttgatttga atatattagt
    13921 gtattccttc agttctgaat cagttttttg tttcatttca tcactcatag attcaatcaa
    13981 atcaacaatc ggtattattt ttttaatctc ctgatcacta tgagttccga aaattctgtc
    14041 tagtagttta cccatttata tctcctaatc tttttaaaac agttgtatat ccttctgcac
    14101 catagtacaa gcttttcgca actctgctta tagttgcagc agatgctcct gtttttaagg
    14161 atatatcagt gtatgtatgt ttttcattaa gcatttttgc aacaacaata cgctgcgcca
    14221 ttgcttttaa ttctgctact gtgcatatgt cttcaaaaaa actataacat tcttctctac
    14281 ttttcaaagt aagaattgca tcaaataaat aatcagattc ttttgattta agtttttcgt
    14341 tcataatgtc acctcaactt ctttctatac tcccaggcag tacttataat atcatgaata
    14401 tcattataac gaggattcca acttagatcc tttttaattt tctcacttga cgcaactaaa
    14461 actgcaggat ctcccttacg tcttggtgca atttctgtct ttatatcttt cttagttaca
    14521 tcttttactg cctttactat ctgatgaact gaaaatccct tttcactccc aaggttatat
    14581 gtcattgaaa gagcacaatt atttgaattc tgcatttttt ccaaagcaag aagatgagct
    14641 gaaactaaat cacaaacatg tatataatct cttatacatg ttccgtctgg agtgtcgtaa
    14701 tcatctccaa aaatcaaaag cttatcgatt ttccctaatg ctacgtccaa ggctattggt
    14761 ataagatgcg tctctatctt ccttttttct cctatacggc cactttcatg actccctgca
    14821 gcattgaaat atctcagtgc cgcataattt ataccataag cttttgaagc ccatttcaac
    14881 attttttcaa ttgaaagctt tgtttctccg tatggatttg tgggattggt tcgagcactt
    14941 tcttttattg gtgatggttg ttcgccataa acggctgcag ttgacgaaaa gacaatgttt
    15001 ttaactccat agttctccat atattgaagt atcttcaatg ttccaaaaac attattttca
    15061 taatacttaa ttggattttg catactctca ccaactagtg agtaagctgc aaaatgtatt
    15121 attccatcaa tattattttc tctaaagact ttatccatgg ctttactatc ttttaaatct
    15181 gcattataga atcgatttgc taatactgct tctttgtttc cggttgacaa atcgtctact
    15241 attacaacat catatccttt ttctaaaaga tgcaacacca tatgcgaacc aatatatcca
    15301 gctccgccag taacaagaat actcatctaa atctccttta cgccatcgga tatattgcat
    15361 acatagaatg atgcatcata tccgattatc tcataataca ctttcgaaat atcatttata
    15421 aactcttcaa ctgattctgt ttttactatg gatactgtac atcctccaaa accagctcct
    15481 gtcattctag aaccaagaac atccttttgc atccaagcgc tttctaccaa aatatccaac
    15541 tctttacatg acacttcata taaatctctt aaggatatat gtgatagatt caaatattta
    15601 cagaacgaga gaatatcatt tttccttaaa gatttgacgc tatccaaaac acgcaaattt
    15661 tcacatacgg catgctcagc tcttttttta catttttcat ccttaatttc atccttacac
    15721 aataaaaatt ccttatatga aaaatcacaa agattcttct tctcaggaaa cttgttttgc
    15781 atatcggcta aagccttttc acattcagaa cgtctttcat tatactttga ttcacctaat
    15841 gatctttttt tattcgtgtt actgattact atggaatatt tgtcaagttt gacaggtata
    15901 tattcatatt ctaaagtgtc agtatcaaga aggattgcat tacctttttt ccccatggca
    15961 gaagcaaatt gatccattat gccacatttg acacctacat actcatgttc agccttttga
    16021 catattaatg cgatctcgat tttatcaata ttgtgtccat agagctcggt catagcataa
    16081 gcagttacaa cttcaattgc tgcagatgaa gacagaccgc ttcccaatgg aaccaagtca
    16141 ttgaatataa actctgcgcc cccaactgat atgcccttct tcatgagctg ttcaattacg
    16201 cctaactgat aattgcccca tttcagattc ttgtattttt caagttcnnn nnnnnnnnnn
    16261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16321 nnnnnnnnnn nnnnnnnnnn nnnnnnngct tgatgcatac acatcatgta aggaaaatca
    16381 aatccaaaaa gattatccag tgttccaaca gcttgtttta gtgttttagc aaaatctgat
    16441 ttctcttttt cagtaaactc tgtaatatat tgtatatgtc gtttggatga tacatatatc
    16501 ccatatggat attcagaaaa gaaaggaagg taaacaataa aactgtcatt ttcaaatata
    16561 actcttttat caaaatcttt ttcattttta ttcatatcac aaataagaca tctgcctgtt
    16621 ttttctgtat gttctttaca tgatgctaat tccagttgga tttttttagg aatgaaagga
    16681 taaccgtaaa tctggccgtg aggatgaggc atagtgacac ctactgcttc tcctctattt
    16741 tcaaaaatga aaacatactt aattttttca tatgtagata tatctttaaa tctatcttgc
    16801 catagattaa ccaattcgtt taaatgcaga tcgtccaatt cgcaaagttt tgcattatga
    16861 tcttttgaat ataaaataac ttcacatttg ccataagctt tatccacttt atacaaatct
    16921 gtttctacat catcaggatt aggagggttt aatgataatg atggaaaatc attatcatat
    16981 ttataaacta taaaatcatc tggaacttta ccgcttccag gacaaaaagg acaccagtct
    17041 ttaggcattt gtggcctgtt ctgtctttgt gacactacca taacccaatc ttttattaac
    17101 ggatgccatc ttaattctgc catatgctac ttttcactcc ttaagttatg ataaatagtt
    17161 gaatgtggag gtacagactt cattaaccat actgatgctc caataactga atcatgaccg
    17221 attacagtat cccctcctag tattgtagca tgtgagtaaa ttatcacatt atcttcaata
    17281 tcgggatgtc ttttgattcc ctttacagga tttccatccg catcaagctc aaaactttta
    17341 gcacctaaag ttacaccttg gtatatttta acattctttc ctattctgca tgtctctcca
    17401 attacaatac cagtaccatg atccataaaa aaatgttctc ctatgcatgc tcccggatga
    17461 atatcaatac ctgttatcct gtgtccatat tcattcatca ttcttggaat taatggaaca
    17521 ttcaatctgt acagctcatg tgctattctg tgaatactca tcatttcaag tgaaggatag
    17581 cttaataata tttcttcaaa gcttttagct gcaggatcac caacatatgc agcatgtata
    17641 tcagtctgaa gcattcgcct tatttcaggt atcttttcta aaaatgaaat aataacagaa
    17701 tcagggtcta tttcacattc tgtattatct tttttgcatt tataatcaaa tactctatca
    17761 attacttctc ttaattctat tgctgcttct ctgatgttat tacctataag catatcaaca
    17821 tttgctttgc ttatagaaca cttctcatat actccaggaa acaatgcgga tcttaaaaga
    17881 aaaaggatat ttttaactct ttccttacct gcaagtgaaa ttgtattttc tcttaaatag
    17941 taatcatcat tcagcgattc aaggtctgat gctatcttcg ggagttcact gtttatccag
    18001 tctccaagtt tattatcttt tctgcccatt tctcctccgt taatttacgt aatacatatt
    18061 atacaacaaa tcttaatttt atgaatattt atctgtaatt
//
LOCUS       DAOSXM010000039        17705 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2111
            NODE_2023_length_17705_cov_11.051615, whole genome shotgun
            sequence.
ACCESSION   DAOSXM010000039 DAOSXM010000000
VERSION     DAOSXM010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864178
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 17705)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17705)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:24:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,261
            CDSs (total)                      :: 2,229
            Genes (coding)                    :: 2,218
            CDSs (with protein)               :: 2,218
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 3 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17705
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2023_length_17705_cov_11.051615"
                     /isolate="AMR_MDS_2111"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..821)
                     /locus_tag="PLQ68_06360"
     CDS             complement(<1..821)
                     /locus_tag="PLQ68_06360"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXK72310.1"
                     /translation="MVRYKYIYNNKKKIWHNKNLYIVILIVLVMSAYIYSSLSGFLGD
                     LKDNQYGNEVIQPGSAHVHYIDVWQGDCILIQDGIHNILIDTGSSIMSSDVLKFFDKH
                     DVDKLDMVILTHPHEDHIGSFDEIAEKYQVDLVLKSGITHDTSTYRNIEKILADEEIK
                     AIVPVYKEIFTYGDMMFTVLSDSSKKYDEINDYSIVVRLDHLGNTFLFTADMESSSEK
                     DLLDKETDIECDVLKVAHHGGSSSTTASFLYEASPVYAIIQSGAGNDYGYPHKSI"
     gene            complement(827..2101)
                     /locus_tag="PLQ68_06365"
     CDS             complement(827..2101)
                     /locus_tag="PLQ68_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005920340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXK72311.1"
                     /translation="MGRKMNKTEWSWVLYDVGNSAFTMLTTALVPIYFAAMVAKTSTI
                     DPNEATALWGTANSIAIMILALLSPILGAIADYRGMKKRMFGVALSVGVLGAFAMAVM
                     NTWLAFLIIYVIARIGYSATNVFYDSMLTDVTTDDRMDRVSSNGFAYGYIGSCIPFIL
                     CLAIYIIQPFGLNDNSAIQVAFVITALWWFFLTIPLFKNVRQTYYLENKKGKVKEAFS
                     RLATTFKNIKEKKDIFIFILAYFFYIDGVYTIITMATIYGTELGLDSVSMVLALLLTQ
                     FIAFPCAIIAGILGTKYGTLKIIKVFIGIYMFVAVFGFFLVTEWQFWVLAVIVGMAQG
                     GIQSLSRGFFGRIIPKKESNEFFGFFDIFGKLADIMGPLLMGLFAIITGHSRYGILSL
                     ILLFAVGFILLSKLPKGFGDIKLKAEDQVLTE"
     gene            complement(2160..2600)
                     /locus_tag="PLQ68_06370"
     CDS             complement(2160..2600)
                     /locus_tag="PLQ68_06370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72312.1"
                     /translation="MDIVKSNKIAYIISLFIGSLVYWSIVSNVIHTAYAITLSAVGYV
                     ICLLFLITHFNEYALNSNNSVMERYYSDRMSVCPKCGNIHVLRYHKVFYSRKFMLLLK
                     LIYSKNAGNVHIVTLVKCDKCNHTWYGGFKNISKIDTQFNKSCI"
     gene            complement(2777..3019)
                     /locus_tag="PLQ68_06375"
     CDS             complement(2777..3019)
                     /locus_tag="PLQ68_06375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72313.1"
                     /translation="MSIKSFFKFRKSAKIEDLTIDPEKINDYIKAREASDKKKTVNEP
                     EGKQDPMLHAVGSNVGGNLAGGINNSVGASVNTITY"
     gene            complement(3187..4437)
                     /locus_tag="PLQ68_06380"
     CDS             complement(3187..4437)
                     /locus_tag="PLQ68_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013411547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HXK72314.1"
                     /translation="MKTKGVRLYGQNDLRLEEFDLPEINDDEILARVTTDSICMSSYK
                     AAIQGEKHKRVPDDVALNPIVIGHEMCGEILEVGKKYKDRYKKGQRFTMQTNLNHPTN
                     VYAAPGYSFRYTGGTIQNMIIAKEVLEMDCLLEYNGDSFYQGSLAEPVSCIIGAFTAM
                     YHTNRINYEHTMGIKEGGKMAILAGVGPMGLGAIDYAVNQEKKPSLLVVTDIDDARLA
                     RAEEIYSVKWAQEHGVKLIYLNTGKTDDPVKALMELSDNKGYDDVFVFAPVKPVVEQG
                     DKILGYDGCLNFFAGPTDPTFSANFNFYNVHYNSSHIVGTSGGNTDDMRKCIRMCEEG
                     KLNPASMVTHIGGLDSAAETTLNLPKIPGGKKLIYTGISMPLTAINELEEKGYPELAK
                     ICKANKGLWCKEAEDWLLKNSKKI"
     gene            complement(4439..5461)
                     /gene="mreB"
                     /locus_tag="PLQ68_06385"
     CDS             complement(4439..5461)
                     /gene="mreB"
                     /locus_tag="PLQ68_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015908663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HXK72315.1"
                     /translation="MGLDIGIDLGTSSVLIYVKGKGVVLREPSVVSKDEDGKIKAVGE
                     DARHMIGRTPANIEAIRPLRDGVISDYEATEEMLKYFIKKVCGKKLLIYKPRIMICVP
                     SGITEVERRAVIDAADKVGAKEVYIIEEPIAAAIGAGLDITKAYGSMVVDIGGGTTDI
                     AVISLCGIVKSKSIKVAGDAFDECVIRFMRKKYSLHIGERMAEEMKIRIGCVYPRKEE
                     VSMQVRGRNLVLGLPRTIEITSSELMEAFDEVIQQIVDGVKNVLEKTPPELSADIGDR
                     GIVLTGGGSLIYGLDTLLSIKTGLEVTIADDPISCVVLGTGEALDNLDKILKLKVVKQ
                     EERSRR"
     gene            complement(5551..6582)
                     /locus_tag="PLQ68_06390"
     CDS             complement(5551..6582)
                     /locus_tag="PLQ68_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012160375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXK72316.1"
                     /translation="MKKALIILILAALVFTFAGCDSDKEVLNVYNWGEYIDMEVIDMF
                     EEETGIKVNYNTFTTNEDLYVKLSKGRVSYDVIFPSDYMIQRMIQEDMLQKISMENVP
                     NYANVDDVHKNLSYDPTNEYSVPYLWGTVGILYNTTMVTDPVDSWDILWNPKYSQKIF
                     MQDSQRDSMMVALIKLGYSQNTTDQSQIDEAMNLLIEQKPLVLGYLVDEVKDKMVQGE
                     GALAVVWSGEAVDAMSMNSDLSYIIPQEGSNRWVDAMVIPKNAKNVSAAEKFINFMLR
                     DDIMLMNAEETGYSITSETAMELLDDDLRNNQAAYPTDEMLEGCEVFEYNAEATVMYN
                     KAWTEVTAH"
     gene            complement(6579..7424)
                     /locus_tag="PLQ68_06395"
     CDS             complement(6579..7424)
                     /locus_tag="PLQ68_06395"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXK72317.1"
                     /translation="MKKILQKIYLFLILLFLYAPIIVLIVYSFNESKSRAIWQGFSLT
                     WYQQLFTDSKILGALETTLLIALVASVVATFLGTFAALGIVKMRKRTFETVRTITYIP
                     VLNPDIVTGVSLMMLFAFIGIELGFVSMLLAHITFCTPYVIISVLPKLYQTDISVYEA
                     AQDLGATPFYALRKTIIPQLMPGIVTGFLLSFTLSVDDFVISYFTTGGHIENLSMVVF
                     SMARKGVNPKINAVSTLLFITVMALLLIVNKRELTSKKRTLKNKETLLDREKQILLIG
                     ENEQK"
     gene            complement(7421..8248)
                     /locus_tag="PLQ68_06400"
     CDS             complement(7421..8248)
                     /locus_tag="PLQ68_06400"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXK72318.1"
                     /translation="MSKRRFSFPYLLWMGIFIVVPLFLVIFYAFTENSSGKIMFTLDH
                     IKAIFEPVYLKVIGRSLYMSLIATVVCFILGYPAAMILASKDFAKKTTMLILIVLPMW
                     MNFLLRTYSWLSLLENEGLINRFLEWMGAGQVQLMYNNFAVVLGMVYNFLPFMILPIY
                     SVLVKIDKNVIEAAKDLGANSYKVFGKVIFPLSVPGVISGITMTFMPALSTFVISRLL
                     SGGKITLIGNIIEQQFTVTGDWGLGSALSLLLMVLIFVSMIFINKYGDESVGAGGLL"
     gene            complement(8245..9333)
                     /locus_tag="PLQ68_06405"
     CDS             complement(8245..9333)
                     /locus_tag="PLQ68_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009252350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXK72319.1"
                     /translation="MGKKIIELKNIYKRYGDFTAIDGINLYFNEGEFLTLLGPSGCGK
                     TTTLRVIGGFEQPDEGDVMFDGVRINDVPAHKRMLNTVFQKYALFTHLTVGQNIAFGP
                     SIKKINPEQIQKDVKKYLKIVNLEGYEDRDISTLSGGQQQRVAIARALINKPKVLLLD
                     EPLGALDLKLRKDMQDELKTMHKDLGITFIYVTHDQEEALTLSDTIVVMNNGKIQQIG
                     TPVDIYNEPNNAFVAYFIGESNIISSTMVRDKVVRIDGVEFECVDKGFGQEMQVDVVV
                     RPEDVILVSPDKSNLKGIVTLITFKGVHYEIWVEGPSFEWKVHSTMPKNIGDELGITV
                     DPYNIHVMHHDPLNIPLEVPPADMGVPI"
     gene            complement(9352..>9783)
                     /locus_tag="PLQ68_06410"
     CDS             complement(9352..>9783)
                     /locus_tag="PLQ68_06410"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HXK72320.1"
                     /translation="RNIITPTIATLQDVLTGLGTNLSEFFSGTYATQIVFKKKDMVTK
                     KDEAEGRQIMWVVPDCQKNAMEPIIISLQPGGMSYQDDPHEGEEFGYVMEGSIKLEIN
                     DKKHTVTKGQSFYFKSDAKHLIRNTTNKKAVVLWISTPPSF"
     assembly_gap    9784..9883
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(9890..11173)
                     /locus_tag="PLQ68_06415"
     CDS             complement(9890..11173)
                     /locus_tag="PLQ68_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HXK72321.1"
                     /translation="MDALVKRSYDKPITDTQLERFLDDAYALFSSKKDVKKILIIPPD
                     FTRFYSMSGKITSMLYKKLKDECTVDILPALGTHMPMTDKELDEMFEGIPHDRFFIHD
                     WKKDVIKIGEVPKEYVYDVSEGLVNEKMDVEVNKMFLDRSYDLILSIGQVVPHEVVGM
                     ANYSKNIFVGIGGNSMINKSHMLGAFYGMERMMGRDFSPVRKVLDYAQEKFISSYPVF
                     YIFTVTTQNGTSADIHGIYAGNGRDAFEEALKDSQQKNFTFLDEEPEKIVVYLDEREF
                     KTTWLGNKAIYRTRMAIANGGELIVLAPEVKRFGEDVENDRLIRKYGYIGRENILKKV
                     EENEDLKNNLSVAAHIIHGSSDGKFNITYAVRHMTKDEIEKANFKYMDYDEAVKKYDP
                     KKLKDGYNMVDGEKIYYISNPALGLWSSRKKFSAQ"
     gene            complement(11175..12026)
                     /locus_tag="PLQ68_06420"
     CDS             complement(11175..12026)
                     /locus_tag="PLQ68_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003467659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXK72322.1"
                     /translation="MKLPFKIDLKNKVVVVTGAGGVLCSMFADALATVKAKVALLDLN
                     YDAAKRISDEINEKGGISIPVKANVLEKESLMAAKQVVNEKLGTCDILINGAGGNNAK
                     ATTTKEYLYKEDMDAEDLVTFFNLDPKGVEFVFNLNFIGTLLPTQVFASDMVGKKGAT
                     IINISSMNAFTPLTKIPAYSGAKAAISNFTQWLAVHFSKVGIRVNAIAPGFFVTNQNE
                     ALLLNKDGTFTERSNKILSATPMNRFGKPEELIGALLYLVDENSSSFVNGIVLPVDGG
                     FSAYSGV"
     gene            complement(12065..13513)
                     /gene="gnd"
                     /locus_tag="PLQ68_06425"
     CDS             complement(12065..13513)
                     /gene="gnd"
                     /locus_tag="PLQ68_06425"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018013701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating NADP(+)-dependent
                     phosphogluconate dehydrogenase"
                     /protein_id="HXK72323.1"
                     /translation="MKKADIGLIGLAVMGENLVLNMESKGFSVAVYNRTVEKVDKFLN
                     TRAKGKNIIGCKTLAELVANLEKPRKVMMMVKAGSAVDDMINQLIPLLSKGDIIIDGG
                     NSNYNDTIDRTKKVENAGLMYIGTGVSGGEEGALTGPSMMPGGTVEAWPHVKKIFQAV
                     SAKVENDIPCCDWVGENGAGHFVKMVHNGIEYGDMELICEAYNIMKTLVGMNSDMMHE
                     VFAKWNEGELDSYLIEITRDILAVKENGEPLVEKILDTAGQKGTGKWTGISALNEGVP
                     LTLIAEAVFARCLSAMKEQRVHASKILTGPKVEFNEEQTAFVEDLKKALYLSKIVSYA
                     QGYELMKAAAVTYDWNLNYGGIALMWRGGCIIRSAFLSKIKEAYDNDPKIPNLLLDPF
                     FKQKADECQMSLRRIVASCAMNGVPVPAFAAALSYYDGYRSERLPANLLQAQRDYFGA
                     HTYERVDSERGIFFHTNWTGRGGDTSASTYNA"
     gene            complement(13611..15059)
                     /locus_tag="PLQ68_06430"
     CDS             complement(13611..15059)
                     /locus_tag="PLQ68_06430"
                     /inference="COORDINATES: protein motif:HMM:NF012931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HXK72324.1"
                     /translation="MHINLVPLPNEFHTDYKLFFRKSIFENTSVEYDSKYSAEGYSIV
                     IDEKGAKVRCSNKAGENFAYQTLKQLEYNYSGNIPYITIEDKPAFSYRGFMIDCARHF
                     FSVEDLKKMIDAACLFKFNKFHWHLVDDQGWRMEIKKYPLLTEKGSVRTHSNFGRYYN
                     PNPHTGYYTVSQMKDIVEYCKKKSIDVIPEIEIPGHASACIHAYPHLSCNQSVIPVRT
                     HAGIFKDILCAGNEKVYEFIFNVLDEMAAIFTSEYVHIGGDEAPKDRWDKCPLCQKKM
                     KELGLTDAEQLQGYMLNRVFSYLNKKGKKAITWNESLRGDNLEKGITVQNWREKNDEC
                     LLRASSGGKIIVSDFFHYYTDYPLGMTSLKKTYEYNPIKETLEESYRKNIIGVETPIW
                     TEFIDNIEKMSTMCYPRFAAVSETGWTLKKNKNYDDFSNRLKKVLPMIEKYGLSYADS
                     YEWDPGFFKSAKQIIKFAYNMYNKETIKSRKE"
     gene            complement(15113..16372)
                     /locus_tag="PLQ68_06435"
     CDS             complement(15113..16372)
                     /locus_tag="PLQ68_06435"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXK72325.1"
                     /translation="MKFDENAKKLTMLCWFSYMINYLARYGFSTSLSDMVADGIFTLE
                     FGGAIGTAFFASYGAGQLVNGLIGDKVHPKFMIFIGLLGSALMNIFVGLSSSHILIII
                     LWCFNGYFCSMLWAPLVRCFSDFLLRKQQLKAGTSISASIPVGNILSYLIASLFLKFM
                     TWRMVFIVSGLIVMVMAFVWFIGMNSLRDYFSEVRIRKLEEIKKETVSVKAPVSGKRL
                     MPLIIGTGVIFAVVGIFFNGILKDGVTLWVPTYVSQFFDTEASTASLVTIILPLFNLI
                     GVYLAVKLFKNVIKNEMMTAAIMFLISVVSLSLLFFFGRYSMIFAVIMLALTTASMLG
                     VNTMFLTFIPLNFSNVGRASSVTGFLDACSYMASALSGVTIGVVASSYGWNVTVLTWI
                     GIAFLGGLVTFLGIKSWKKGRFMLTGQ"
     gene            complement(16408..>17705)
                     /locus_tag="PLQ68_06440"
     CDS             complement(16408..>17705)
                     /locus_tag="PLQ68_06440"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXK72326.1"
                     /translation="ESAISVNEYLFSKDTFMGMDIKQLSKTADELGLNPDNIYSDRLL
                     GSFSGGEIVKIRLLKVLSQRPDVLLFDEPTNDIDIDTLVWLECFINKCNIPLMYVSHD
                     ETLIENTANVIIHMETRKRKMECIHTISRLSYSEYIKNRMHNLEKQDQIASMQRKEYK
                     KQINKWRKIHDEVERQQENISRQDPGGGRLLKKKMKSVKSMEKRFEREKEDFADFSDA
                     EEAIFIRFYEDSYIDKSRKVLDICIDELMIENTVLAKDIELYVKGGEHIGIVGNNGVG
                     KTTFLKEILKRIPKREGIRVGYMPQDYEDLMNLDSSCMDFLVPGMKKEDVTRARQYMG
                     GMKFTPSEMLNKISSLSGGQKAKLFFLKMILEGCNVLVLDEPTRNLSPLSNPVIRKSL
                     TAFPGTIISVSHDRKFLVEVCHTVYTFTKEGLSQKQSLC"
BASE COUNT         5350 a         3687 c         2497 g         6071 t
ORIGIN      
        1 attatactct tatgcggata tccgtagtca ttaccggctc ctgactgaat tatggcataa
       61 acaggagatg cttcgtataa aaaagaagca gttgttgaag aactccctcc gtgatgtgcg
      121 actttaagaa catcacattc aatatctgtt tctttatcta gaaggtcttt ttcagaactg
      181 ctttccatat cagctgtaaa caaaaatgta tttcctaagt gatctagtct gacgactatg
      241 gaatagtcat taatttcatc atactttttg gatgaatcag ataaaactgt aaacatcata
      301 tcaccataag taaatatctc tttatatacg ggcactattg cttttatttc ctcatctgct
      361 aatattttct caatatttct ataagtactg gtatcatggg taataccact tttcagaaca
      421 agatcaacct gatacttttc tgctatctca tcaaaagacc ctatatgatc ttcatgcgga
      481 tgcgttagaa tcaccatatc taatttatct acatcatgtt tatcaaaaaa tttcaacaca
      541 tctgatgaca ttattgagct tcctgtatcg atcaaaatgt tgtgtatacc atcctgaata
      601 agaatacagt caccctgcca gacatctatg tagtgaacgt gagcggagcc cggctgaata
      661 acctcatttc catattgatt atctttaaga tcacccaaaa accctgataa agatgaatat
      721 atgtatgctg acataacaag cacaataaga atcactatat ataaattctt attgtgccat
      781 attttctttt tattattata tatatattta tatctaacca ttacacctat tcggtcagca
      841 cctggtcttc cgctttaagc ttaatatccc caaaaccctt tggaagtttg gaaagaagaa
      901 taaaacctac tgcaaataat aaaatcaaag aaagtattcc atatcttgaa tgaccggtta
      961 taatggcaaa cagacccatt aacaaaggac ccataatatc tgcaagcttt ccaaagatat
     1021 caaagaaacc gaagaattcg tttgattcct tctttggaat aattcttccg aagaatcctc
     1081 ttgaaaggga ctgaatacct ccttgagcca tacctactat aactgccaat acccagaatt
     1141 gccattcagt aaccaggaaa aacccaaaaa cagctacaaa catatatatt cctataaaca
     1201 cctttataat cttcaaagta ccatatttgg ttcctaatat acctgctata atagcacatg
     1261 ggaatgcaat aaactgagtc agaagcaaag ctaataccat ggatactgaa tcaaggccta
     1321 attctgtacc gtaaatagtc gccatggtta ttatcgtata aactccatct atataaaaga
     1381 aatatgcaag tatgaaaata aaaatatcct ttttctcttt aatattctta aaggttgtag
     1441 caagtcttga aaaagcttct ttaactttcc cttttttatt ttcaagataa tatgtctgac
     1501 gaacattctt aaataacgga attgtaagga aaaaccacca caatgcagtt attacaaaag
     1561 caacttgtat tgctgaatta tcattcagac caaatggctg gataatatat attgccaaac
     1621 ataatataaa cgggatacaa ctgcctatat acccatatgc aaatccattt gaggataccc
     1681 tgtccattct atcgtcagta gttacatcag ttagcatgga gtcataaaat acatttgttg
     1741 cagaatatcc gattctggct ataacgtata taatcaaaaa tgccaaccat gtattcataa
     1801 ctgccatagc aaatgcacct aacactccaa ctgacagggc aacaccgaac attctttttt
     1861 tcatacctct gtaatcggcg atagctccaa gaattggtga aagcaaagcc agtatcatta
     1921 tggcaataga attagcagtt ccccatagcg cggttgcttc attaggatca attgtactgg
     1981 tttttgcaac catcgcagca aagtaaatag gaacaagtgc tgttgtaagc atagtaaatg
     2041 cggaattacc aacatcatac aaaacccagc tccattcggt cttgttcatt tttctaccca
     2101 ttattaaatt ccctccattt gtacctgtta atgatattct accatcaatg gagtttgttt
     2161 taaatacaac ttttattaaa ttgcgtatca attttcgata tattcttaaa tccgccatac
     2221 catgtatgat tgcacttgtc gcatttaact aaagttacaa tatgtacatt tcctgcattc
     2281 tttgaatata tcaattttaa caaaagcata aattttctgc tatagaagac cttgtgatat
     2341 cttaaaacat ggatatttcc acatttagga catacagaca ttctatctga ataatacctt
     2401 tccattactg aattattact gtttaaagca tattcattaa aatgcgttat caagaataat
     2461 agacatatta catatccaac agctgaaaga gttatagcat atgcagtatg aataacattg
     2521 ctgactatac tccaatacac taaagagcct ataaaaagag aaattatgta tgctatcttg
     2581 tttgatttca ctatatccat tacatgtacc acctttctta aggtatacac ataatatcat
     2641 gcttattgta ctatatattg gactttattt acctattttt gatttattaa aaagccagcc
     2701 tatttaagct gactcttgtt tgtgcaattt ttaaatcaat atcattacag gtaaaacata
     2761 agattataga aatcttttag taagttattg tattaactga ggctcctact gaattattta
     2821 taccgcctgc taaattacct ccaacatttg aacctacagc atgtaacata ggatcttgtt
     2881 tcccttctgg ttcattaact gtttttttct tatcagaggc ttctcttgct ttgatgtaat
     2941 catttatctt ttcaggatca atggttaaat cttctatttt tgcacttttt ctgaacttga
     3001 aaaaactctt aattgacatc ttattcaacc tccttttttt tggtgtgcat ttattatatt
     3061 atatatcgca ttatttgtca ataatttgac actcaattat gacataactt aaaatgcacc
     3121 ttactattca gtaaataaaa gagcaggctt ccctgctcct ttttgattaa tattcaaacc
     3181 attagtttaa atctttttac tgttttttaa taaccagtcc tctgcttctt tacaccataa
     3241 gcctttattg gctttgcata tttttgctaa ttcaggatat cctttttctt ctaattcgtt
     3301 tattgctgtt aaaggcatgc tgatacctgt gtatataagt ttctttccac ctggtatctt
     3361 tggaaggttc aaagtagttt cagctgctga atccagcccg cctatatgtg taaccattga
     3421 agcaggattt aattttcctt cttcacacat tcttatgcat tttctcatat catctgtgtt
     3481 cccaccgctt gttcctacta tatgacttga attatagtgg acattgtaga aattgaaatt
     3541 tgctgagaat gttggatctg tcggacctgc aaagaaattc aagcacccgt catatcctaa
     3601 aattttgtca ccctgttcaa caaccggttt aacaggtgcg aaaacaaata catcatcata
     3661 tcctttatta tctgaaagtt ccatcagggc tttaacagga tcatctgtct ttcctgtatt
     3721 aagataaata agctttactc catgctcttg agcccattta actgaatata tttcttcagc
     3781 tcttgcaagt cttgcatcat caatgtcagt tactactaat aacgatggtt tcttttcctg
     3841 gtttactgca taatcaatag cacctaatcc catagggcca actcctgcga gtatagccat
     3901 ctttccgcct tcttttattc ccatagtatg ttcataattt attctattgg tgtgatacat
     3961 cgcagtaaag gcaccaatta tacatgatac cggttcagct aaagaaccct gatagaaaga
     4021 atcgccattg tactctagca gacaatccat ctccaatact tccttggcaa taatcatatt
     4081 ctgaatagta ccgcctgtat acctaaatga atacccaggt gcagcatata cattggtagg
     4141 atggttaaga ttagtctgca ttgtaaaacg ttgtcccttt ttatatctgt ctttatactt
     4201 ttttcctacc tcaagtattt caccacacat ctcatgacct ataactatag gatttaaagc
     4261 tacatcatca ggaactcttt tatgcttttc accctgaatc gctgctttgt aagatgacat
     4321 acagatactg tctgttgtta ctcttgctaa tatctcatca tcattaattt ctggtaaatc
     4381 gaattcttca agccgaagat cattctggcc ataaagcctt acaccttttg ttttcatttt
     4441 atcttctgct cctttcttcc tgttttacaa cttttagttt taatatctta tctagattat
     4501 ccaatgcttc tcctgttcct aaaacaacgc aggatatcgg gtcatctgct attgtaactt
     4561 ccaatccggt cttaatagac aacagagtat ccagaccata tatcagactt ccaccacctg
     4621 ttaaaactat tcctctgtcg cctatatctg cagaaagctc aggaggtgtt ttttccagta
     4681 cattcttaac gccatcaact atctgctgaa taacttcatc aaaagcttcc attagctcag
     4741 aagaagtgat ttctatggtt cttggcagtc ctaaaacaag atttctgcct cttacctgca
     4801 tagatacttc ttcttttctt ggatatacgc atcctatgcg tatcttcatc tcctcagcca
     4861 ttctttcacc gatatgaaga ctgtatttct ttctcataaa tctgattaca cattcatcaa
     4921 acgcatcacc agctacttta attgatttgc tcttaactat accgcacaag gatattacag
     4981 ctatatcagt agtacctccg cctatatcga caaccatact tccataagct ttggtaatat
     5041 caagaccggc acctattgca gcggctattg gttcttctat aatatatacc tcttttgccc
     5101 ctactttgtc ggcagcatct ataactgctc ttctttcaac ttcagtaatt ccacttggaa
     5161 cacatatcat tatacgaggt ttataaatta aaagcttctt gccgcatacc ttttttataa
     5221 agtatttaag catttcttca gtagcctcat aatcagatat aacgccatct cttagaggtc
     5281 ttatagcttc aatatttgca ggagttctgc ctatcatgtg gcgggcatct tctccaaccg
     5341 cttttatttt gccatcttca tccttcgata ctactgaagg ctcacgcaaa accacgcctt
     5401 ttcctttaac atatataagt actgaagaag taccaagatc tataccaata tctagtccca
     5461 aaacgttgct ccttacatct tattgtttta attatatact attactaatg caataaatac
     5521 ctatttttca caataaatat taatatttgc ttaatgtgca gtaacttcag tccatgcttt
     5581 attatacatt acagtagctt cagcattata ctcaaatact tcacatcctt ctagcatttc
     5641 gtcagtcgga tatgccgctt ggttgttcct taaatcatcg tcaagcagtt ccatagcggt
     5701 ctcacttgtt attgagtaac ctgtttcttc tgcattcatt aacattatat catctcttag
     5761 catgaagtta ataaactttt ctgcagcaga tacatttttt gcattctttg gtattaccat
     5821 agcatcaacc catctgttgc ttccttcctg cggaattata taggacagat cagaattcat
     5881 gctcatagca tctacagctt ctcccgacca aactactgct aaagctcctt caccctgaac
     5941 catcttgtcc tttacttcat ctacaagata acctaaaacc aaaggctttt gttcaattaa
     6001 aagattcatt gcttcatcta tctggctctg gtctgttgta ttttgtgaat atccaagttt
     6061 aataagcgca accatcattg aatcgcgctg gctgtcctgc atgaatattt tctgggaata
     6121 tttcggattc caaagaatat cccagctgtc aacaggatca gttaccatag tggtgttata
     6181 tagtatacct actgtacccc ataaatatgg tactgaatat tcattagttg gatcataaga
     6241 gagattttta tgcacatcat caacatttgc ataattaggg acattttcca tactgatctt
     6301 ttgtagcata tcttcttgta tcattctttg aatcatgtag tctgaaggga atatcacatc
     6361 atatgatacc cttcctttgg ataacttaac atacagatct tcgtttgttg taaatgtgtt
     6421 gtaattaacc ttgataccag tttcctcttc aaacatatcg ataacttcca tatcgatata
     6481 ttcaccccag ttgtaaacat tcaaaacttc cttgtcactg tcacagcctg caaatgtaaa
     6541 tactaaagct gcaagaataa gaataataag tgcttttttc atttttgttc attctctcct
     6601 attaataaaa tttgtttttc tctatctaat aacgtctctt tattcttcaa agtacgtttt
     6661 ttcgatgtca gttcccgttt gttaacgatc aaaagtaaag ccattacagt tataaataac
     6721 aaagttgaaa ctgcatttat ctttggattg accccttttc ttgccattga gaagacaacc
     6781 attgaaagat tttcaatatg tcctcctgta gtaaagtagc ttataacaaa atcatcaacg
     6841 cttaatgtaa aagataaaag gaatcctgta acaataccag gcataagctg cggtattata
     6901 gtctttctta aggcataaaa aggtgttgct cctaaatcct gagctgcttc ataaacagat
     6961 atatctgtct gataaagttt tggaagaaca gaaattataa cataaggtgt gcagaatgtt
     7021 atgtgcgcaa gaagcattga tacaaagcct aattcaatac cgataaatgc aaaaagcatc
     7081 attaaagata cgcctgttac aatatcagga ttcagtactg gtatatatgt aatagtcctt
     7141 accgtctcaa aggtcctctt tctcattttc actatgccta aagctgcaaa tgttcctaaa
     7201 aatgtggcta ctactgaagc tacaagtgca atgagcaaag tagtctccag cgcacctaat
     7261 attttactgt ctgtaaaaag ctgctgatac catgtcagcg aaaatccctg ccagatagca
     7321 cgtgattttg attcattgaa tgagtaaaca ataagcacga ttattggagc atatagaaag
     7381 agcagtatca ggaaaagata aattttctga agtatctttt tcataacaga cctcctgctc
     7441 cgactgactc atccccgtac ttattaatga atatcataga tacgaaaatc aataccatca
     7501 gcaaaagaga taatgctgaa cccagtcccc agtcacctgt aacggtaaac tgctgttcta
     7561 ttatatttcc tattaaagtt atctttcctc cgcttaaaag ccttgatatg acgaacgtgg
     7621 acaaagcagg catgaatgtc attgttatac ctgatataac cccaggaaca cttaaaggaa
     7681 atattacttt cccgaatact ttatagctgt tagcgcctaa gtccttagct gcctcaataa
     7741 catttttatc tatttttaca agtactgaat aaataggcag aatcatgaat ggtaaaaaat
     7801 tgtaaaccat gcctaatact actgcaaaat tgttatacat caattggacc tggcctgctc
     7861 ccatccattc aagaaatctg tttattaacc cttcgttttc caaaagtgaa agccatgagt
     7921 atgtccttaa aagaaaattc atccacattg gaagaacaat aagtataagc atagttgttt
     7981 ttttggcaaa atccttgcta gctaaaatca ttgcagcggg ataccctaat ataaagcaga
     8041 ctacagtagc tattaatgac atgtataatg aacgaccgat tacttttagg tacaccggtt
     8101 caaatattgc ttttatatgg tcaagagtaa acattatttt tccagaagaa ttttctgtaa
     8161 aggcataaaa aattactaga aaaagaggaa cgacaataaa aatgcccatc cataataaat
     8221 agggaaagga aaatcttctt ttactcatat tggcactccc atgtcagcag gaggaacttc
     8281 taaaggaata ttcagaggat catgatgcat aacatgaata ttatatggat caacagtaat
     8341 tcctaactca tctccaatgt tcttaggcat agttgaatga accttccatt caaatgaagg
     8401 tccttcaacc catatctcgt aatggacacc cttaaaagta attaatgtaa ctatcccctt
     8461 aaggttggat ttatcaggag atacgagtat tacatcttca ggccggacta caacatccac
     8521 ctgcatttcc tgaccaaaac ctttatctac acattcgaac tccactccat caatacgtac
     8581 taccttgtct cttaccattg tactgctgat aatattactc tctcctatga aatatgctac
     8641 aaaagcatta tttggttcgt tatatatatc gactggcgta cctatctgct gtatcttccc
     8701 attattcatt acaacaattg tatcggacaa tgttaatgct tcctcttggt catgagtaac
     8761 ataaataaag gttatcccta aatctttatg cattgtcttg agttcatcct gcatatcttt
     8821 tctgagtttt aaatccagag ctcctaaagg ttcatctaaa agcagaactt ttggtttgtt
     8881 aatcagcgcc cgggctattg caactctttg ctgctgccct ccgcttaaag tagaaatatc
     8941 cctgtcctca tatccttcaa gattaactat cttaagatac ttttttacat ccttctgtat
     9001 ctgttcagga ttgatttttt ttatagaagg tccgaaagct atattctgac caacagtaag
     9061 atgagtaaaa agagcatatt tctgaaatac tgtattcagc atccttttat gagcagggac
     9121 atcatttatc cgaacgccat caaacataac atcaccttca tcaggttgct caaatcctcc
     9181 gattactctc aaggttgtag tttttccgca acctgaaggt cctaataatg ttaaaaattc
     9241 accttcattg aaatacagat taataccgtc aatagcagta aaatccccat atctcttgta
     9301 aatgttcttc aactcaataa tctttttacc catttgctct cctatatgta attaaaaact
     9361 gggcggtgtt gaaatccata acacaaccgc tttcttgttt gtagtatttc ttattaaatg
     9421 cttagcatca gatttaaaat agaaagattg cccttttgta actgtatgct tcttgtcatt
     9481 tatttctagt tttatgcttc cttccataac atatccgaat tcctctccct catgagggtc
     9541 atcctgatag ctcattcctc caggttgtaa tgaaataatt attggttcca tggcattctt
     9601 ctggcagtca gggactaccc acataatctg ccttccctct gcttcatctt tttttgttac
     9661 catatccttt ttcttaaaaa ctatctgagt agcatatgta cctgaaaaaa attcagaaag
     9721 attggttcct aaaccagtta acacatcctg cagtgtggca attgtaggtg taataatatt
     9781 tctnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnattattc tattgcgctg
     9901 aaaatttctt tcttgatgac caaagaccta atgcaggatt ggagatatag taaatttttt
     9961 ctccatcaac catgttatat ccgtctttca gcttttttgg atcatatttc tttacggctt
    10021 catcataatc catgtatttg aaattagctt tctcaatctc atcttttgtc atatgacgta
    10081 ctgcataggt aatattgaat ttgccatctg atgaaccatg aataatatgc gctgcaactg
    10141 aaagattgtt tttcaaatcc tcattttctt caaccttttt caatatgttt tctctgccga
    10201 tatacccata tttacgtatt agcctgtcat tttcaacatc ctcgccaaaa cgcttaacct
    10261 caggtgctaa aacaataagc tctccaccat tggctatagc cattcttgtt ctgtatatag
    10321 ctttatttcc cagccatgtt gtcttaaatt ctctttcatc aagataaaca acaatttttt
    10381 ctggttcctc atctaagaaa gtaaaattct tctgctggct gtcttttaac gcttcttcaa
    10441 aagcatctct gccattacca gcatatattc catgaatatc tgctgaagtt ccgttctgtg
    10501 tggtaacagt aaaaatatag aaaacaggat atgaggatat gaacttttcc tgcgcgtaat
    10561 ccagtacctt tctcacagga ctgaagtctc ttcccatcat tctttccatc ccataaaaag
    10621 ctcctaacat atgcgattta tttatcatac tgtttccgcc gatacctaca aatatattct
    10681 ttgaatagtt agccattcca actacttcat gaggtacgac ctgacctatt gataatatga
    10741 ggtcatatga cctgtctaaa aacatcttat ttacttctac atccatcttc tcattaacaa
    10801 gaccctcaga aacatcatac acatactctt ttggaacttc tccgattttt atcacatcct
    10861 ttttccagtc atgtatgaaa aatctgtcat gcggaattcc ttcaaacatc tcatcaagct
    10921 ctttatctgt cattggcata tgtgttccta atgcaggaag aatatctact gtgcattcat
    10981 cttttagttt tttatacagc attgatgtga ttttccctga catggaataa aatcttgtaa
    11041 aatcaggagg aattataagt attttcttaa catccttttt agatgaaaat aatgcatagg
    11101 catcatctaa aaacctttca agttgggtat cagttatcgg tttatcataa cttcttttta
    11161 ctaaagcatc catatcatac tccgctatat gccgagaagc cgccatctac tggtaatact
    11221 ataccgttta cgaaacttga tgaattctcg tcaactaaat acagcaatgc gcctataagt
    11281 tcctctggtt ttccaaaacg attcataggg gtagctgaca ggattttatt ggacctttct
    11341 gtaaatgtac catccttatt cagcaataac gcctcattct gatttgttac aaaaaagcca
    11401 ggagctatcg catttactct tattccaact tttgaaaaat gtactgctaa ccattgagta
    11461 aaattagata ttgcagcttt agcaccagaa tatgcaggta ttttggtcaa tggggtaaat
    11521 gcgttcattg atgatatatt tattatcgtt gcaccctttt taccaaccat atctgatgca
    11581 aatacctgtg taggcagtaa agtacctata aaattcaaat taaatacaaa ttccacaccc
    11641 tttggatcaa gattgaaaaa agtaaccagg tcctcagcat ccatatcttc cttgtaaaga
    11701 tattctttag tcgtagtagc tttcgcattg ttcccgccgg ctccatttat tagaatatcg
    11761 catgttccta atttttcatt tacaacctgc tttgcagcca taagactttc tttttctaaa
    11821 acattagctt tcacaggaat tgatatgccg cctttttcat tgatttcatc agaaatcctt
    11881 tttgctgcat catagttcaa atctagcaaa gcaacttttg cttttaccgt tgctaatgcg
    11941 tctgcaaaca tggaacataa aacgccacct gctccagtta caactactac cttgttcttt
    12001 aaatctattt taaatggcaa tttcataatt ttcctcctta taagatatta ttatcaattt
    12061 attattaagc attatatgta gaagcggaag tgtcaccacc tcttccagtc cagtttgtat
    12121 ggaagaaaat tcctctttca ctatctactc tttcatatgt atgtgctccg aaatagtcgc
    12181 gctgtgcctg taaaagattg gcaggcagtc tttcgcttct atatccgtca taatatgata
    12241 aagctgctgc aaatgcagga actggaactc cgttcatcgc acatgatgca acaattcgtc
    12301 ttaagctcat ctggcattca tcagctttct gcttaaagaa aggatctagc agaagattag
    12361 gtattttcgg atcattgtca tatgcttctt ttattttact taagaaagca gaacgtatta
    12421 tacatccgcc tctccacatt aaagctatac ctccgtaatt tagattccag tcatatgtaa
    12481 cagcagctgc tttcataagc tcatatcctt gtgcatatga tactattttt gaaagataca
    12541 aagctttttt caagtcttca acaaaagctg tctgttcttc attaaattct actttaggtc
    12601 ctgtaagtat cttacttgca tgtactcttt gttctttcat tgctgataag caacgtgcga
    12661 aaactgcttc tgctattaat gtaagaggaa ctccttcatt taatgcagat ataccagtcc
    12721 attttccagt acctttctgt cccgctgtat caagtatctt ttctactagt ggttcgccgt
    12781 tttccttaac tgccaatata tctcttgtta tttcaatcag ataagagtca agctcccctt
    12841 cgttccattt tgcgaatact tcatgcatca tatctgaatt catgccgaca agcgtcttca
    12901 tgatattata tgcttcacat ataagctcca tatcaccata ttcaattccg ttgtgtacca
    12961 ttttgacgaa atggcctgca ccgttctcgc ctacccagtc acagcatggg atatcattct
    13021 caacttttgc tgacacagcc tggaatattt ttttaacatg cggccatgct tctactgtac
    13081 cgcctggcat cattgaaggt cctgtaaggg caccttcttc tccaccgctt acaccagtcc
    13141 ctatatacat aagtcctgca ttttccactt ttttagttct atctatagtg tcgttatagt
    13201 ttgaatttcc gccatctatt attatatcgc ccttactaag aagcggtatt aactggttta
    13261 tcatatcatc aacagcagaa cctgctttca ccatcatcat gacttttctt ggtttttcaa
    13321 gatttgcaac cagttcagca agggttttgc acccgataat atttttgccc tttgctcttg
    13381 tatttaagaa tttatctact ttttctactg tcctgttgta cacagctaca ctaaagccct
    13441 tactctccat attgagaacc agattctctc ccattacggc aagacctata aggcctatat
    13501 ctgctttttt catatattac ctctttcttt aagggcacat ataaataccc gtttatttta
    13561 catatagatt gtataataca gacgggtttt tgtctattga taacaaccga ttattctttt
    13621 cttgatttga ttgtttcttt gttatacata ttgtaagcaa acttaattat ctgttttgca
    13681 gatttgaaaa agcctgggtc ccactcataa ctgtcagcat atgacagacc atatttttct
    13741 atcatcggaa gaactttttt aagtctgttt gaaaaatcat cataattctt attctttttc
    13801 aaagtccatc ccgtttcact gacagctgca aatcttggat agcacatagt agacattttt
    13861 tctatattat caataaattc agtccaaata ggagtttcaa ctcctataat attttttctg
    13921 taactttcct caagtgtttc tttaattgga ttatattcat aagtcttttt tagagaagtc
    13981 atacctaatg gataatccgt ataatagtga aagaaatcgg aaactatgat tttaccgcct
    14041 gaacttgctc taagtaaaca ttcatcattc ttctctcgcc agttttgaac agttataccc
    14101 ttttccagat tatctcccct taagctttcg ttccatgtta ttgccttttt ccctttttta
    14161 ttaagatagc tgaataccct gttaagcata tatccttgca gctgttctgc atctgtcaac
    14221 cctagttcct tcatcttttt ttggcataac ggacacttat cccatctgtc tttaggtgct
    14281 tcatctcccc ctatgtgaac atattcagaa gtgaaaattg cagccatttc atctaggaca
    14341 ttgaatatga actcatatac cttttcatta cctgcacata gaatatcttt aaatatgcct
    14401 gcatgggttc ttaccggtat aactgactgg ttacatgaaa ggtgaggata tgcatgtatg
    14461 catgctgatg catgcccagg aatctcaatt tccggtatta catcaatact tttcttctta
    14521 cagtattcaa ctatgtcttt catctggctt acagtatagt atccagtatg aggatttgga
    14581 ttgtagtatc ttccaaaatt tgaatgtgtt cttactgaac ctttctcagt aagcaacgga
    14641 tatttcttta tttccattct ccaaccctga tcgtccacca aatgccagtg aaacttatta
    14701 aacttgaata aacatgctgc atcaatcatc ttttttaagt cttcaacaga aaagaaatgt
    14761 cttgcacagt ctatcatgaa tcctctgtat gaaaaagccg gtttatcttc aattgtgata
    14821 tatggaatat tgccagaata gttatactca agctgtttta atgtctggta tgcgaaattc
    14881 tctcctgctt tatttgagca tcgaactttt gcgccttttt catctatcac aatagagtaa
    14941 ccttcagcgg aatacttaga atcatattcc acggatgtat tttcaaatat gctttttcta
    15001 aagaaaagct tgtaatcagt gtgaaactca ttaggcagag gtacaagatt aatatgcata
    15061 acaatcctcc aaatttttac attaatatat atattctacc acaaaaactt gattattgac
    15121 cggtaagcat aaaacggcct tttttccatg atttaattcc taagaatgtt actaaacctc
    15181 ctaggaaagc tatccctatc catgtcagta ctgtcacatt ccatccatat gaacttgcaa
    15241 caactcctat agttactccc gataaggccg atgccatata tgaacaggca tctaaaaaac
    15301 cagtaactga tgatgctctg ccgacattgg aaaaatttag aggtatgaat gtcagaaaca
    15361 tggtatttac gccaagcatt gaagctgtcg tgagtgcaag cattattact gcaaatatca
    15421 tagagtatct tccaaagaag aagagaaggc taagtgaaac tacacttatt aaaaacatta
    15481 tcgcagctgt catcatttcg ttcttaataa cattcttaaa aagctttact gcaagatata
    15541 cacctataag attaaatagc ggcagtatga ttgtaactag tgaagctgtt gatgcttcgg
    15601 tatcaaagaa ctgacttaca tatgtaggaa cccataaagt tactccgtct ttgagtattc
    15661 cattaaagaa tatacctaca actgcaaata tgacccctgt ccctataatc aaaggcataa
    15721 gccgttttcc cgatacagga gcctttactg atacagtttc cttctttatt tcttctagct
    15781 ttctgattct aacctcagaa aaatagtccc ttaatgaatt catccctata aaccatacaa
    15841 aagccattac cattactatt agtcctgaaa caataaagac cattctccat gtcataaatt
    15901 tcaaaaatag cgaagcaata agataagaca gtatatttcc cacaggtatg cttgcagata
    15961 ttgatgtgcc tgccttcaac tgctgttttc ttagaagaaa atccgaaaaa catcttacta
    16021 aaggtgccca taacatagaa cagaaatatc cgttaaaaca ccataatatg attattaaga
    16081 tatgagagga agacagtcca acaaatatat tcattaatgc agaacctaaa agccctatga
    16141 atatcataaa ttttggatga actttgtccc ctattaaccc attcacaagc tgtcctgccc
    16201 cataagatgc aaaaaacgca gtacctattg caccaccgaa ttccagtgta aatataccat
    16261 ctgcaaccat atcgctaagt gatgttgaaa aaccatatct ggcaagatag ttaatcatgt
    16321 atgagaacca gcagagcatg gtaagttttt tagcattttc gtcaaatttc aaattaatac
    16381 caatcctagt aatactaagt cagtatatta acacaatgat tgtttctgtg aaagaccttc
    16441 ttttgtaaat gtgtaaacag tatgacaaac ttcgactaaa aatttcctgt catgtgatac
    16501 tgaaattata gttccgggaa aagcagtcag actttttcta ataacaggat ttgataaagg
    16561 gcttaagttt cttgttggtt catctaaaac taaaacattg caaccttcta aaatcatttt
    16621 aaggaaaaac agtttggctt tttgccctcc tgacaatgaa gatattttat taagcatttc
    16681 agatggtgtg aatttcatgc ctcccatata ctgccttgct ctcgtaacat cttccttttt
    16741 catgcctggg actaaaaaat ccatacatga actatccaaa ttcattagat cctcgtaatc
    16801 ctgaggcata tatccaaccc ttataccttc tcttttaggt attcttttaa ggatttcttt
    16861 taaaaaagtt gtttttccta caccattatt tccaacaatt cctatatgtt cccctccttt
    16921 tacatatagt tcgatatctt ttgccaaaac cgtattttca atcataagct catcaataca
    16981 tatatcaaga acttttcttg atttgtctat gtatgaatcc tcataaaacc ttataaatat
    17041 agcttcctct gcatcagaaa aatcagcaaa atcctctttt tccctttcaa atctcttttc
    17101 catagatttg actgacttca tctttttttt aagaagtctt cctccaccag gatcttgtct
    17161 tgaaatgttt tcctgctggc gttcaacttc atcatgaatc tttctccatt tatttatctg
    17221 ctttttgtac tcttttcttt gcattgatgc aatctgatct tgtttttcca gattgtgcat
    17281 cctgttttta atatattctg agtagcttaa tcttgaaatg gtatgaatac attccatttt
    17341 cctttttctt gtttccatat gtattattac atttgctgtg ttttctatca gggtctcatc
    17401 atgagaaaca tacattaaag gtatattgca cttattgata aaacactcca gccacactaa
    17461 tgtatctatg tcaatatcat tagtaggttc gtcaaataaa agcacatcag gcctttgaga
    17521 aagcacctta agcagtctga ttttaacaat ttcacctcca gaaaaactac ccagcaacct
    17581 gtcagaatat atattatccg gattcaagcc aagttcatct gctgtttttg atagttgctt
    17641 aatatccatt cccataaatg tatcttttga aaaaagatat tcattaacac ttattgctga
    17701 ttctg
//
LOCUS       DAOSXM010000040        17665 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2111
            NODE_2028_length_17665_cov_10.071153, whole genome shotgun
            sequence.
ACCESSION   DAOSXM010000040 DAOSXM010000000
VERSION     DAOSXM010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864178
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 17665)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17665)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:24:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,261
            CDSs (total)                      :: 2,229
            Genes (coding)                    :: 2,218
            CDSs (with protein)               :: 2,218
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 3 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17665
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2028_length_17665_cov_10.071153"
                     /isolate="AMR_MDS_2111"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..877)
                     /locus_tag="PLQ68_06445"
     CDS             complement(<1..877)
                     /locus_tag="PLQ68_06445"
                     /inference="COORDINATES: protein motif:HMM:NF019924.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HXK72327.1"
                     /translation="MKILYVSTEGTPFAKAGGLADVIGALPYSVAAKGHETAIILPYY
                     KSIRDKWKYEAKFLTSTIVTVGWRKQYAGILTLKHKGITIYFIDNEFYFGSDKIYSYG
                     DYEAERMAFFNRAVLESLKLINTSYDILHLNDWQTAMIALLLKDQYIKNPVYNGFYDN
                     LRTVFTIHNLKYQGIYESSVLPDLFNLSMDYAVIDKLEFFGRINFMKAGLIYSDKITT
                     VSNTYKEEIRNPFFGENLDGVINQRNLDLYGIVNGLDEEEYDPKTDKFIYQTFGIRDI
                     EKKNTNKMMLQKQLDL"
     gene            complement(877..2001)
                     /gene="glgD"
                     /locus_tag="PLQ68_06450"
     CDS             complement(877..2001)
                     /gene="glgD"
                     /locus_tag="PLQ68_06450"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR02092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
                     /protein_id="HXK72328.1"
                     /translation="MKDALGIIFGEGTDAGLGTLTANRSLAAVPIASRYRIIDFILSS
                     MVNSGITNVGIITQKNYKSLMDHLGTGKEWDLNRKIGGLLLIPPFSSRDTMSNENGDL
                     SDLAGALDFLEKSPQKYVVLSKSHLICNLNFDDAFEKHVNTNSIVTIFYQNLEPRGEV
                     SGLKNRFFLNLDEDDRVNQIKAYSSTNLSNKISLDIYIINKEFLIYLIRDGLASGEKG
                     SLSEILSKRVDHMRMTGYIAKDFVARIDSIKTFYQANMVILNKDIRERLFFRPGRIYT
                     KVKDEAPTKYMDSAVVKNSLIADGCVINGYVENSIIFRGVKIEKGAKVINSIIMQNSE
                     VMEDAILEHVILDKEVIIRKDIRLTGQQSYLVLINKGAIV"
     gene            complement(2010..3212)
                     /locus_tag="PLQ68_06455"
     CDS             complement(2010..3212)
                     /locus_tag="PLQ68_06455"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013916580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HXK72329.1"
                     /translation="MQNKDCIALILAGGQGSRLGVLTKKVAKPAVNFGGKYRIIDFPL
                     SNCTNSNIDTVGVLTQYQPLELNSYIGSGQPWDLDRSFGGVYLLPPYMKSESGTWYKG
                     TANAIFQNMNFIQMFNPRNVLILSGDHIYKMNYAKMLEAHNKSDADVTISCIRVPWDQ
                     ANRFGIMETDRNLRIKNFFEKPENPISNMASMGVYIFKWEILKDYLVKDEQDEKSSND
                     FGKNIIPKMLKDGRRMYAYKFKGYWKDVGTIYSLWEANMELLHKKPAMNLFDKGWRIY
                     SKNAIQPPHYTAEGAVIKDSMVTEGSVIYGKVKNSIISSGVRIGKNSTVEDSVLFPGV
                     VIEDECIVKKAIIGERSVVMEGSQIGVDDNIVGQTPSKMCSDGIVLIGTDLIIESGTK
                     IAKNVMVE"
     gene            complement(3221..>4594)
                     /gene="glgB"
                     /locus_tag="PLQ68_06460"
     CDS             complement(3221..>4594)
                     /gene="glgB"
                     /locus_tag="PLQ68_06460"
                     /inference="COORDINATES: protein motif:HMM:NF008967.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HXK72330.1"
                     /translation="ANELIPYIKHMGYTHIELMPIMEYPFDMSWGYQCLGYFCANSRH
                     GSPKQLKYFIDCCHQNDIGVILDWVPAHFPKDSHGLAQFDGSSLYEYSNPKKAHHNEW
                     GTLVFDYGKKEVCSFLLSSAYFWLKEYHADGLRVDAVSSMLYLDYGRRAGEWEPNEKG
                     GRENLEAVHFLRSLSKSLFRDFPNIILAAEESTAWPNVTSPVCDGGLGFNFKWNMGWM
                     NDTLSYVSKDPIFRKFDHHKLTFSMVYAFSENYILPLSHDEVVHGKKSLLDKMPGSYE
                     DKFSNLKIYYAYYISHPGKKLLFMGGEFGQFIEWRYQNELDWMLLDYESHRKLKEFVR
                     DLNIFYKRHKAFYEQDRDWIGFRWINADADAESILSYIRRDKKGKNDIIILINFTPVD
                     RYGYRIGVPYSGQYKILLNSSDEKYSMQGKKIEKVICSETIPWDGLNYSICVDLKGLT
                     ALYIKKD"
     assembly_gap    4595..4694
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4695..4939)
                     /locus_tag="PLQ68_06465"
     CDS             complement(<4695..4939)
                     /locus_tag="PLQ68_06465"
                     /inference="COORDINATES: protein motif:HMM:NF014919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HXK72331.1"
                     /translation="MKIISGFDMHLFREGTQCQAYKMFGAHLTDNGCEFSVYAPNAEY
                     VSVVGDFNDWDIFSHNMNRDDNGIFSIFIPEAKEWDR"
     gene            complement(5329..6198)
                     /locus_tag="PLQ68_06470"
     CDS             complement(5329..6198)
                     /locus_tag="PLQ68_06470"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HXK72332.1"
                     /translation="MKIGVSSYSYEKLFRKGLIDSFGIIKQTKDFGFDAIEFSGIPGD
                     TYDKRYQLAKQIKKACDDVDLEIANYSTGADLIKGSDWDIEKETDRIKKEIDIANILG
                     VSKMRHDASFGFSANDPKARVLDFYIEKIAKGCTILTEYAKQANIRTMIENHGKYCQH
                     SQDVLKIYNAVNNDNFGILIDIGNFLCVDQNPIDGVSDLASYAFHVHAKDFHYKAGYN
                     MNPGTGWFLTKQGNYLRGSIIGHGEVPLKECINIMKTYGYNDVLSIEFEGIEDVMMAI
                     ENGYDNLKRLVNL"
     gene            complement(6210..7028)
                     /locus_tag="PLQ68_06475"
     CDS             complement(6210..7028)
                     /locus_tag="PLQ68_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019004101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HXK72333.1"
                     /translation="MDFKIGVITDSFRCSLDKAIDKAASLGADGVQIYATGRELSADM
                     SSSEIKKVVNHLNNAGLELSALCGDIGAFDDAIVNVERIRRSKRIVDLAVNMGTKIVT
                     THIGTVPEDKNHEKYKIIHNACSELGEYADKSGVKFAIETGPEKAFVLRMLLDDLDCS
                     GIAVNFDPANLTMCVDDDAREAVSILAPFIVHTHAKDGIMLSKYTNDQRYSQIAENKK
                     WLELPLGEGDVIFPSYLDALRKIGYNGFLTIEREVGENPEKDIANAVSFLRELI"
     gene            complement(7087..8142)
                     /locus_tag="PLQ68_06480"
     CDS             complement(7087..8142)
                     /locus_tag="PLQ68_06480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72334.1"
                     /translation="MISNEIKKRYNAFWDRTSYERCILFLSRRLKSGTVMEGVSVKDK
                     WTDIERRTRAAAENCEITKYYAEGFPTIFTNFGPGCMSACIGGDFVLAESTIWFDRNP
                     IITNWEKVPKILFDDNSEMWKLTYELTKMLGKESLGRYHTSISDIGGSLDIVASLRGT
                     LELLYDLVDYPEQVKKVHLVVEEIWKEVYTLLYNESIKYQEGMTSWMPIWCKKRYYPL
                     QCDFCAMISPDMFDEFVKPSLVSQTEWLDHSIYHLDGPGEVCHLDSLLDIQRLDAIQW
                     TPGDGRRPISDEFYFDMYNKILRSGKGLVLFSVPLSGLENLLKNISTKGLYVNMSVES
                     DEEAEYAIKIAQSYGVK"
     gene            complement(8158..8937)
                     /locus_tag="PLQ68_06485"
     CDS             complement(8158..8937)
                     /locus_tag="PLQ68_06485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72335.1"
                     /translation="MYDKKDLDVFGLDESFTRQELEYQYSLMLKKAKADPSYDISRAS
                     EAYNKILGIKEPEEISPKEQKKRDRKMKLAENLIFFIAGGIVVLILAIAIPSAIMKKS
                     IDLHICISGQFTVDDSEIIKQNIRPEVKPRKTSVEFIYASFGTGDDSASYSIQALSLS
                     LLAGDYDLMITDLPVYGFLTANPQDILILELSDYLDELGLKESDERLVKSYGGIYGIK
                     VLSSQALDGIYTNQGYYYLSMPNRADDIENALEAIKRIINN"
     gene            complement(8950..10242)
                     /locus_tag="PLQ68_06490"
     CDS             complement(8950..10242)
                     /locus_tag="PLQ68_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HXK72336.1"
                     /translation="MKFNLLHPADQIVMLMERIYRYGMTTTSGGNLSVLDDNGDIWIT
                     PSGIDKGSLQRKDIMQIKSDGTIIGPHTPSVELPFHAHVYKTRADAKAIIHAHSPASV
                     AFSIVRQIPNVHLVPNINKICGEVGMAEYDVPGSEALGKKISKSFKNGYDTVMMENHG
                     VIVIGANLFDAFMKFETIDMCAKLEIRAKNLGTIKPLSQKHIDISLQKKGEIMQDFVP
                     SGYSSDERAIRREMCELIHRSYDQQLFTSTQGTFSVKLDDNSFVITPYGVDRKYISVE
                     DLVRIENGKYKEAGKTPSRSVALHKHIYDSQPHIKSIIISHPPNMMAFAVTDTQFDAH
                     TIPESYIALRNVPKLPYGSSFLQPAMFAKIFDKKTPVVLVENDCVIVTGSTLLQAFDR
                     LEVAEYSAKALIDTTVLGDMIKINEEQTKEIERIFKLD"
     gene            complement(10294..11850)
                     /locus_tag="PLQ68_06495"
     CDS             complement(10294..11850)
                     /locus_tag="PLQ68_06495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72337.1"
                     /translation="MSGLMINWDDSAFFYYISKGYGKDIKDSKQAYECAKAVIDQYEN
                     TAVTDFIMCVNARLASYPSKTIENYGDSYRRKVQNGINVDYSDTWASSFHKFFEIWGI
                     DHFQIWIDRLNELNIKPWISIRMNDAHQSLEKTSLLAPDYYYENPHLRRVTHHKTQGY
                     FDGLYDYGKKQVRDRFLNFVDETLARYDVYGIELDWMREALCFKIGHEHEGVEILNDF
                     MMNVKKIKDKYEKIRNHKISISARVPSGIETSFYMGFDVVTWAEKKLIDVVVISPRWS
                     STDTDMPIEIWKRMLMPFNVELATGIEILIRQNRTYPLTMHSKETVAANAAVAFSAGA
                     DKFYLFNFMSVPGLVGIDESNEAHRQLFCDLKELLNSCSDLENSLKYTRRHFVTFKDF
                     TALWESSKYYVPVTCEPDDTEYALFRIRTGKVSEKSKAYILMGIDTDNNANLSDEDIT
                     VFLNSYKLEGIRISDVNEDYYIKSSIYILEIPNITFLNDINTVEVCANKIKFTIKHIE
                     IDIDNCINEN"
     gene            11944..13317
                     /locus_tag="PLQ68_06500"
     CDS             11944..13317
                     /locus_tag="PLQ68_06500"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HXK72338.1"
                     /translation="MICIISLMSKSENRKILKLLISVGLPIAIQNLLSNGINLIDNFM
                     VGSLSEDALSAVSLANRSYFIFIMLLFGLISGIGIYSSQYYGSKEIPKIHSMMGIGFT
                     FGVSFAVLLTILISLFPDDTVRIFIKDESVVSIGTEYIKIAVLSFIPASISMVFQINL
                     RSVRMTKIPMMTSLVAAIFNGLLNYVLIFGKLGFPALGVKGAAIATVTARFIELVMVL
                     LFVFFNKNCPLKGTIKSYFSYTLPMMVTVFRRSLPVVLQEGLWAIGTSVYFIAYGNLS
                     STSVAAAQISLTSLDIYISLFIGLGNGAGVMVGNLLGEKQIEKAQRYSNKIILYQIIL
                     SIPFAILFSFSGIWITRFLNFSVAGNQATLYTIYVLAAYLPIKCMNFLFIVGILRGGG
                     DTRFCMISETASVWLIGVPLAFISVLVLKLPIYWCVALVNLEEITKLILCSVRYKSKK
                     WINVLVD"
     gene            13328..14125
                     /locus_tag="PLQ68_06505"
     CDS             13328..14125
                     /locus_tag="PLQ68_06505"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF019887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HXK72339.1"
                     /translation="MYKLIATDMDGTLLNDEGNLPSNTFNAARFLEDNGIKLVLCTGR
                     PLLGLIKIYDSLNLTSPIVANNGACAYLDKSGPLIMNQTMSIEDGLAVIKLNEIFNCT
                     ICMWHNQILYVSEFNKYAYDYASITGVTPLHLSKIDLKKEKIGPTKFVFRDTLEKITS
                     IKESAAELSPPSVTAVTTRPHFLEFFSSSISKAAALEKIGQTLNISKDEMIAAGDGFN
                     DIEMMDYVGLAVAVQNAADEVKKHADVIGCSNNEGIIDYIVNTIIKK"
     gene            complement(14180..17320)
                     /locus_tag="PLQ68_06510"
     CDS             complement(14180..17320)
                     /locus_tag="PLQ68_06510"
                     /inference="COORDINATES: protein motif:HMM:NF013256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HXK72340.1"
                     /translation="MYFDQQHIRKFLNDMNTMRFTDLADVNDIRIADVEGKINLKLLK
                     DTDISKMSQYEPGTIYENEGNKYHIFKFKLKVSPKYDKLPLVLKMITGFGYEWNEHRN
                     PNFLLFVNGEPLQAFDYGHHECIITDCAKAYDEYDCFIFEFSGMKKGPVQMSLKTCSL
                     DKPTEKLYYDILCPAESIQFIESEAIRKDTTKYLINAINMVDLRNPYSEEYYDSVNKA
                     DNYLIDQYYNNYCDDDTSKVMISAIGHTHIDVAWRWPLKETRQKTVRTFSTQLKNMND
                     YDSFRFIASQPQLYKYLEEDYPEMIDKIKEAVENGQWEAEGAMWLESDCNLVSGESFV
                     RQIMHGKRYIKKMFNKDSRLLWLPDVFGYSAALPQILKKSGVDYFSTSKISWNEYNVI
                     PYNTFMWEGIDGSEVFTNFIVGGSGPDEFGKPRVNYNARMNAENVMGTWKNYRDKSLS
                     KSPLMTFGLGDGGGGPSRDHIEQCNRFSKGIKGMPKIENVRALDYFDRLYEEVKEDKR
                     LPKWVGELYLEYHRGTYTSVAKNKKNNRKSEILLRDTEVFSAIADKLGLDTYDKKKLY
                     DIWDIVLLNQFHDIIPGSSIKEVYDDSDIDYARVMKDGKELLSSALHAFSKTVKGKEL
                     CVVFNQLAFKRVDTIRIRTDKDYTCVYDSVNNMIPGQMVNEDNKKYFIFEARLCPLGY
                     TVFKLSSDDVDLSDNFFKATDNMMENAYLKVKFDENMNISSLYDKTANREVILDGQTA
                     NRITAYEDKPIKYQAWDINIYYNEKPYDIDNVTYTELVEDGPIRKCIRIDRKFLETTI
                     TQYVYLEKSSKKLDFKTIIDCNHDDILIKAAFPVDVHASEAAYDIQFGNVKRPTHWNT
                     SWDYARFEVCAHKWADLSEDDYGVSLLNDCKYGHDIKGSVLRLTLLKSAIEPYPDADR
                     GLHEFTYSLYPHTGDFKKAHTIDMAYKLNYRPYAYYVEKGTGNVENYKSFITCNDENV
                     IVDTLKKAEDTDDLVLRVYECFNRRTDCTIDFGFDVSKITECDLMENDICNVEFENNT
                     LKFNIKPYEIKTFKLS"
     gene            complement(17335..>17665)
                     /locus_tag="PLQ68_06515"
     CDS             complement(17335..>17665)
                     /locus_tag="PLQ68_06515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72341.1"
                     /translation="KYGNPFDCEMKKVFDCMRTLTGDDVNYCPIEAAIPQLKTINAIS
                     QYYNISQISSDRLGYLDAEENTTKLVYVKGLYEDMMSAYENNAILDESSRIIDITEYD
                     KFENIMK"
BASE COUNT         5434 a         3412 c         2520 g         6199 t
ORIGIN      
        1 caaggtctag ctgtttttgt agcatcattt tgttggtatt ctttttttcg atatccctta
       61 taccaaaagt ctgataaatg aacttgtcag ttttaggatc atattcttct tcatcaagac
      121 cattaacgat tccataaagg tccagattcc tttgatttat aacaccatct aaattctctc
      181 caaaaaatgg atttcttatc tcttctttat atgtattgct gactgtagtt atcttatctg
      241 aatagataag ccctgctttc ataaaattga ttcttccaaa aaattctagt ttgtctatta
      301 ctgcataatc cattgataaa ttaaaaaggt caggaagaac agaggactca taaattccct
      361 gatactttaa attatgtata gtgaaaacag tccttaagtt atcgtagaag ccattataga
      421 ctggattctt tatatactgg tccttaagta acagagctat catagcagtc tgccagtcat
      481 taagatgcag aatatcatat gatgtattaa taagtttaag tgattctaaa acagctctgt
      541 tgaaaaaagc cattctttcc gcttcataat cgccataact gtatatttta tcggaaccaa
      601 aataaaattc attatcaata aaatatatgg tgattccttt atgttttaag gttagtattc
      661 cagcatactg ttttctccat ccaacagtta caatggtact agtaagaaac ttcgcttcat
      721 atttccattt atctctaatg cttttatagt atggtagaat tatcgctgtt tcgtgccctt
      781 tcgcagctac agaatacggc agagctccaa tgacatcagc taatcctcca gcctttgcaa
      841 aaggtgttcc ttcagttgat acatataaaa ttttcattac acaatcgctc ctttgttaat
      901 caatacaaga tatgactgtt gccctgtaag tctgatatct tttctaataa tgacttcctt
      961 atcaagaatg acatgttcca atatcgcatc ttccataact tctgagttct gcattattat
     1021 cgaatttata acttttgctc ctttttcgat tttgacacct ctaaatatta tgctgttttc
     1081 aacatatccg tttataacac atccatctgc tattaaagag tttttaacta ctgcagaatc
     1141 catatattta gttggtgctt catcttttac tttcgtataa atccttccag gtctgaaaaa
     1201 tagtctctcc ctgatatcct tattcagtat aaccatattt gcctgataaa aggttttaat
     1261 ggaatcaatt cttgcaacaa aatcctttgc gatataaccg gtcattctca tgtgatctac
     1321 tcttttagac agtatctcac ttagagaccc tttttcccca gatgctaatc catctcgaat
     1381 tagatatatt aaaaactcct tattaattat atatatgtca agtgatatct tgttactaag
     1441 attagtagag gaataagcct taatctgatt aactctgtca tcttcatcga gatttaagaa
     1501 gaacctgttc ttcaatccgc ttacctcacc tcttggttca agattctgat agaaaattgt
     1561 aacaatagag ttagtattaa catgtttttc aaatgcatca tcaaaattca aattacaaat
     1621 caaatggctt ttagaaagta caacatattt ttgaggtgac ttttctaaaa aatccagagc
     1681 tcctgccagg tcagacaaat ctccattctc attgctcatt gtatctcttg aagagaatgg
     1741 cggtatcaga agcaatccgc ctattttcct gttaaggtcc cattcttttc ctgtaccaag
     1801 atgatccatt agtgatttgt agtttttctg agttattatt ccaacattag ttattcctga
     1861 attgaccatt gatgataaaa taaaatcaat aatcctgtat cttgaagcta tggggactgc
     1921 agccaatgat ctgttagctg taagtgttcc caatcctgcg tctgtgcctt caccaaaaat
     1981 tattcctaaa gcatctttca tattatcacc tactccacca ttacattctt agcaatcttc
     2041 gttccgcttt cgattataag atcggtgcca ataagcacaa tcccgtcaga acacatctta
     2101 gaaggagtct gccccacaat attatcatca acacctatct gtgatccttc cataactact
     2161 gaccgctcgc cgattattgc tttttttact atacactcat cttctattac gacacctgga
     2221 aaaagaactg aatcttccac agtagaattt ttaccaatac ggactccgct ggatattata
     2281 ctgtttttta ctttgccgta aatgacactg ccttcggtca ccatactatc tttgattact
     2341 gcgccttcag ccgtataatg cggaggttga atagcatttt tcgaatatat tctccagcct
     2401 ttatcaaaaa gattcattgc tggcttttta tgtaaaagtt ccatgttcgc ttcccataaa
     2461 ctatagatag taccgacatc cttccagtat cctttaaatt tataggcata cattcttctt
     2521 ccatctttga gcattttggg tattatattc ttaccaaagt catttgatga tttctcatcc
     2581 tgctcatctt tgacaagata atcctttaaa atttcccatt taaaaatata tactcccatt
     2641 gatgccatat tacttatagg attctctggt ttttcaaaaa agtttttaat acgcaaattt
     2701 ctgtctgttt ccataatacc gaaacgattg gcttgatccc atggtactct tatacacgaa
     2761 atagtaacat cagcatcgct cttgttatga gcttcaagca ttttcgcata attcatttta
     2821 tatatgtgat ctccagatag aataaggaca ttacgcgggt taaacatctg aataaaattc
     2881 atattttgga atattgcatt tgcagtacct ttgtaccacg tcccactctc tgatttcata
     2941 tatggaggca aaagataaac cccgccaaaa cttctgtcaa gatcccatgg ctgaccgctt
     3001 cctatatatg agttcaattc caaaggctga tactgagtaa gaaccccaac agtatcgata
     3061 tttgagttgg tacaattact aagcggaaaa tcaattattc tgtatttccc accaaaattc
     3121 actgcaggtt ttgcaacttt tttagtcagt actcctaaac gacttccttg cccccctgct
     3181 aatataagcg ctatacaatc tttattctgc atatgacctc ctagtccttt tttatatata
     3241 aagctgtcag tccttttaaa tcgacacata tactgtaatt caaaccatcc cagggaatag
     3301 tttcactaca tattactttt tctatttttt tcccctgcat agaatatttt tcgtctgatg
     3361 aatttaataa gattttatac tgtcccgagt atggaacacc tatcctgtat ccgtatctgt
     3421 caacaggtgt aaaattaatc agtataataa tatcattttt acctttttta tctcttctta
     3481 tatatgacaa tatgctttct gcatccgcat cggcattaat ccatctaaat cctatccagt
     3541 ccctgtcctg ctcataaaaa gctttatgac gtttgtaaaa aatattcagg tcacgaacaa
     3601 attctttaag ttttctatgt gattcataat ctaaaagcat ccagtcaagc tcattttgat
     3661 atctccattc aataaactgt ccgaattctc cacccatgaa taaaagcttc tttccaggat
     3721 gcgaaatata gtaagcataa tatatcttta gatttgaaaa tttatcttca tatgagcctg
     3781 gcattttatc gagtaatgat tttttaccat gtactacttc atcatgtgat aaaggaagaa
     3841 tataattttc cgagaatgca taaaccattg aaaatgttaa tttgtggtgg tcaaattttc
     3901 gaaaaatcgg gtctttagaa acatatgata aagtatcatt catccatccc atattccatt
     3961 taaaattaaa gcccaaaccg ccgtcacaaa caggcgatgt tacattaggc caggctgttg
     4021 attcctcagc agctaaaatt atgttaggaa agtctcggaa aagactctta gataatgatc
     4081 ttaaaaagtg cacagcttca agattctctc ttccaccttt ttcattaggc tcccattcac
     4141 ctgctcttct tccataatca agatacaaca tggaagacac agcatcaacc cttaaaccat
     4201 cagcatgata ctcctttagc cagaaatatg cgcttgagag cagaaaactg caaacttctt
     4261 ttttaccata atcaaaaact aaggtgcccc attcattatg atgtgctttt ttgggattag
     4321 aatattcata taagctgctt ccgtcgaatt gtgctaaacc atgcgaatct tttggaaaat
     4381 gagcaggaac ccagtctaat attaccccaa tatcattttg atggcagcaa tcaatgaagt
     4441 atttgagctg ttttggtgaa ccatgtctgc tattcgcaca aaaataccct aagcactggt
     4501 atccccatga catgtcaaaa ggatattcca ttattggcat cagttctata tgcgtgtatc
     4561 ccatatgttt aatatatggg attaattcgt ttgcnnnnnn nnnnnnnnnn nnnnnnnnnn
     4621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4681 nnnnnnnnnn nnnntatctg tcccactctt tagcttcagg tatgaaaata ctgaatattc
     4741 cattatcatc cctattcata ttatgggaaa aaatatccca gtcattgaaa tcccctacaa
     4801 cagaaacata ttcggcattc ggagcatata cagaaaactc acacccattg tcagtcaggt
     4861 gcgctccgaa catcttgtat gcttggcatt gagtcccttc acggaacaaa tgcatgtcaa
     4921 agccagaaat gattttcatg cttacctcgt actattattc taacatataa aaaaggaata
     4981 atacataata ttatcccttt cataactaca tataacagca aatagtgctt ttatgaatat
     5041 cttataactt aagaaatata aacatctgta ttaaggcagc taaaaaccca gcaacaagac
     5101 caatccatat tattatttta attacaatgt tttgcttttt cgtaaaatat tcaggatttt
     5161 ccttcaagat aagcaaaatg cagacaaaca taagacatag gcataatgcg cttacagatg
     5221 gtatgaatat ctctcctatt aaatataaca agctgaacac aatgccgcct aatgcaaaac
     5281 aaagggtgca tattaataat attttatttt taactgtttt attactcatt aaagattaac
     5341 cagtcttttc aaattgtcat aaccattctc tatagccatc attacatctt caatgccttc
     5401 aaattcaatg gaaagaacat cgttatatcc atatgtcttc ataatgttta tacattcctt
     5461 taagggaact tccccatgac caataattga acctctgaga taattaccct gttttgtcag
     5521 aaaccatcca gtacctgggt tcatattata tccggctttg taatggaaat cctttgcatg
     5581 aacatgaaac gcataggaag caagatctga tactccatcg atagggttct ggtcaacaca
     5641 caaaaaatta cctatatcaa tcaaaatacc aaaattgtca ttattaactg cattgtaaat
     5701 cttgagtaca tcctgactat gctggcagta tttaccgtga ttttcaatca tagtacggat
     5761 attagcttgt ttagcatatt cagtaagaat agtacaacct ttggctattt tttctatgta
     5821 aaaatcgaga acacgtgctt ttggatcatt tgcagaaaat ccaaatgaag catcatgacg
     5881 cattttggat acacctaaaa tattagctat atcgatttct tttttaattc tgtctgtttc
     5941 tttttcaata tcccagtctg accctttaat caaatcagct ccagtagaat aattagctat
     6001 ttctaaatca acatcatcac aagctttttt tatctgtttg gctaattgat atcttttatc
     6061 atatgtatca cctggaatac ctgaaaattc tattgcatca aacccaaaat cctttgtctg
     6121 tttaattatt ccaaatgaat caattaatcc ttttctaaat agcttttcat atgagtatga
     6181 actgactcct attttcatta tttcctcctt caaattaact ctcgtaaaaa tgatacagca
     6241 tttgcaatat ctttttcagg attttcgcca acttctcttt ctattgttaa gaatccatta
     6301 tatccaattt tcctcaacgc atctaaatat gatggaaata ttacatcacc ttcacctaat
     6361 ggaagctcta accatttctt gttttctgct atttgactgt atctttgatc attggtgtac
     6421 ttgcttagca taataccatc ttttgcatga gtatgaacaa tgaaaggtgc aagaattgat
     6481 actgcttctc ttgcatcatc atcaacgcac attgtaagat ttgcaggatc aaaattaact
     6541 gcaatacctg agcaatccag atcatcaaga agcattctta atacaaatgc tttttcagga
     6601 ccagtttcaa ttgcaaattt taccccggat ttatccgcat actcccctaa ctcactgcat
     6661 gcattatgaa ttattttata cttttcatga tttttgtctt ctggaacagt acctatgtgg
     6721 gtggtaacaa ttttagttcc catattaact gcaaggtcta ctattctttt tgatcttctt
     6781 attctttcaa catttacaat agcatcatcg aatgcaccta tatcaccaca taatgcagac
     6841 aattccaaac ctgcgttatt taaatgattt acaacttttt ttatttcaga tgaactcata
     6901 tcagcagata attctcttcc tgttgcatat atctgaactc catcagcccc taatgatgct
     6961 gctttatcaa ttgccttatc caacgaacac ctaaacgaat ctgtaataac cccaatttta
     7021 aaatccattt tatcctccaa attatttaaa tcaatttata aataaaacat caattagtaa
     7081 cttgctttat ttaactccgt aactctgtgc aattttaatt gcatattcag cttcctcatc
     7141 actttctact gacatattaa cgtataatcc ttttgtagaa atattcttaa gtaaattttc
     7201 aagaccagat aatgggacgg aaaataggac aagaccttta ccacttctta atatcttgtt
     7261 atacatatca aaataaaact catcagatat tggccttctt ccatctcctg gtgtccattg
     7321 aatagcatcc agtctttgaa tatccaataa tgaatccaga tgacatacct ctccaggtcc
     7381 gtcaagatga tatatagagt ggtcaagcca ttctgtttgc gaaacaagac ttggttttac
     7441 aaattcatca aacatatcag gggaaatcat tgcacagaaa tcacactgta acgggtaata
     7501 tcttttcttg caccagatag gcatccagct tgtcattccc tcttggtact taatactttc
     7561 attatataaa agagtatata cttccttcca tatttcttct accactaaat ggactttttt
     7621 tacctgctca ggataatcaa caagatcata aagcaattca agagtacctc ttaaggaagc
     7681 tactatatcc agagatcctc ctatatctga gatggaagtg tggtaacgtc ctaatgattc
     7741 tttaccgagc atttttgtta attcataagt cagtttccac atctcactat tatcatcaaa
     7801 caaaatcttt ggtacttttt cccaatttgt aattattgga ttcctgtcaa accagattgt
     7861 tgattctgct agaacaaaat caccgccaat acacgctgac atgcagccag ggccgaagtt
     7921 agtaaaaata gtgggaaaac cttcagcata atactttgtg atctcacaat tttctgcagc
     7981 tgcacgtgtt cttctttcaa tatcagtcca cttatctttt actgaaacac cttccattac
     8041 agtacctgat ttcagtcttc ttgagaggaa taatatgcat ctttcatatg atgttctgtc
     8101 ccaaaaagca ttatatctct tctttatttc attacttatc ataaattccc aatcccccta
     8161 attattaatt attcttttta tagcttctaa agcattttca atatcatcag ctctgtttgg
     8221 cattgataag taataataac cttgattggt atatatccca tctaaagcct gacttgataa
     8281 taccttaatt ccataaattc cgccataaga ttttacaagt ctttcatccg attctttcaa
     8341 tcctaattca tccagataat cagataattc taaaataagg atatcttgag gattagcagt
     8401 cagaaaacca taaaccggta gatcggttat cataagatca tagtccccag ctaaaagaga
     8461 tagagatagc gcttgtatag aataagatgc tgaatcatct cctgttccga aagatgcata
     8521 tatgaactca actgatgttt ttcttggctt gacttcaggt cgtatgtttt gcttaattat
     8581 ttcgctatcg tcaactgtaa actgacctga gatacagatg tgaagatcaa ttgacttttt
     8641 cattattgca gacggtattg ctattgccaa tataagaacc actatacctc ctgcaatgaa
     8701 gaatataagg ttttctgcca gtttcatttt tctgtcacgt tttttctgtt ctttaggtga
     8761 aatttcttca ggttccttta ttccaagtat tttgttgtat gcctcactcg ctcgggaaat
     8821 atcgtaagaa ggatcagctt tagctttttt aagcataaga gaatactgat actccagttc
     8881 ctgccttgtg aaactctcat ctaatccgaa aacatcaaga tcttttttgt catacatagg
     8941 actgtcctcc tagtctaatt taaatatcct ttcaatttct tttgtctgtt cttcatttat
     9001 tttaatcata tctcctagta cggtagtatc tataagagct ttagcactat actctgctac
     9061 ttctaaccgg tcaaatgcct gtaaaagggt ggaacctgtt actataacac aatcattttc
     9121 cacaagtaca actggagttt tcttgtcaaa tatttttgca aacattgcag gttgcaggaa
     9181 actggaccca tacggaagtt taggcacatt tcttaatgcg atatagcttt caggaattgt
     9241 atgtgcatca aattgcgtat ctgtaactgc aaaagccatc atatttggcg ggtgtgaaat
     9301 aattatgctt tttatatgcg gttgagaatc atatatgtgt ttatggagag ctactgacct
     9361 tgatggagtt tttcctgctt ctttgtattt accattttca attcttacaa gatcctcaac
     9421 tgaaatatat tttctatcga caccataggg agtaataacg aatgaattat cgtccaattt
     9481 gacagaaaaa gtaccttgtg tgctggtaaa aagctgctga tcgtatgatc tatgtattag
     9541 ctcgcacatt tctcttcgaa tagctctttc atcagaagaa tatcctgaag gtacaaaatc
     9601 ctgcataatc tctccttttt tctgtaatga gatgtcaata tgtttttgag acaaaggttt
     9661 tattgtaccc aaatttttag cacggatttc aagcttagca cacatatcaa tagtttcaaa
     9721 tttcataaat gcatcaaaaa gattagctcc tattacaata acaccatgat tctccatcat
     9781 tacagtatca taaccgttct taaaagattt tgaaatcttc tttcctaaag cctcactacc
     9841 tgggacatca tattcagcca taccaacttc accgcatatc ttatttatat tcggtactaa
     9901 gtgaacatta ggtatctgac gaactatact gaaagcaact gaagctggag aatgagcatg
     9961 aataattgct tttgcatctg ctctagtctt atatacatga gcatgaaatg gaagttccac
    10021 cgaaggagta tgaggtccga ttattgttcc atcagatttt atttgcataa tatccttcct
    10081 ttgaagacta cctttatcaa tcccgcttgg agttatccaa atatcaccat tatcatcaag
    10141 aactgataag ttacctccgc ttgtcgtagt cattccatat ctgtatattc tttccatcag
    10201 cattactatc tgatctgctg ggtgcagtaa gttgaattta atcatttatt tttctgtgat
    10261 acaatatctt attgaatcac actccccttt ccgttaattt tcatttatac aattatcaat
    10321 atctatttct atatgcttta tggtaaattt aattttgttg gcgcatactt ctaccgtatt
    10381 tatatcattt aaaaatgtga tatttggaat ttccagaata taaatagatg attttatata
    10441 ataatcctca ttcacatcag aaattctaat tccctcaagt ttataactgt ttagaaaaac
    10501 agttatatcc tcatctgaca agttagcatt gttatcagtg tcaataccca tcagaatata
    10561 tgcttttgac ttttcggata ccttccctgt tctaatccta aataatgcat attctgtatc
    10621 atcaggctca catgttacag ggacataata ttttgatgat tcccataaag ctgtaaaatc
    10681 cttaaaggta acgaaatgcc ttctagtata ttttaatgag ttttcaagat cgctacagct
    10741 gtttaataat tcctttaagt cacaaaaaag ttgcctatgc gcttcattag attcatcaat
    10801 tccaactaat cctggaacac tcataaaatt gaagagataa aatttatctg cacctgcact
    10861 gaatgccact gctgcattgg cagctacagt ttctttagaa tgcatcgtta aaggatatgt
    10921 tcggttctgt cttattagta tctctatacc agttgctaat tctacattaa atggcattaa
    10981 catccttttc cagatttcta taggcatatc agtatcagtt gaagaccatc ttggtgatat
    11041 tacaaccaca tcaataagtt ttttttctgc ccatgttaca acatcaaatc ccatatagaa
    11101 agatgtttct ataccagatg gaacccttgc ggaaatacta atcttgtgat ttctgatttt
    11161 ttcatattta tctttaattt ttttaacatt catcataaaa tcatttaata tttcaacacc
    11221 ttcatgttca tgtcctattt taaaacacaa tgcttcgcgc atccagtcca gttcaattcc
    11281 atacacatca tatcttgcca gagtttcatc aacaaagttc aagaacctat cacgcacttg
    11341 ttttttgcca taatcatata acccatcaaa atatccttgt gtcttgtggt gagttactct
    11401 tctaagatgt ggattttcat aataatagtc tggtgcaagt aaagaagttt tttctagtga
    11461 ctggtgtgca tcattcatcc ttattgatat ccaaggtttt atattaagtt cattaagtct
    11521 gtctatccat atttggaagt gatctatacc ccatatttca aagaatttat gaaatgaaga
    11581 tgcccaggta tcagaataat caacatttat gccattctgt acttttcttc tgtaagaatc
    11641 accataattt tcgattgtct ttgatggata cgaggctaac ctcgcattta cacacattat
    11701 aaagtcagta acagctgtat tctcatattg atcaattact gcttttgcac attcatatgc
    11761 ttgtttgcta tcttttatat cttttccata tccttttgat atgtaataaa agaaagcgct
    11821 atcgtcccag tttatcatta atccactcat aaagctctcc tattccttta cgattatatc
    11881 atgaatattc aattgattat ttaaaataga ttaaatattt aaaaagtgat gtaattaagt
    11941 tgtatgatat gtataatatc ccttatgagc aaaagcgaga acaggaaaat ccttaaatta
    12001 ctcatatcag tgggattacc cattgcaata cagaaccttt tatctaatgg tataaacctt
    12061 atagataatt tcatggtggg cagtttatct gaagatgcgt taagtgctgt aagtcttgca
    12121 aaccgatctt atttcatttt tataatgctt ctattcggac ttataagcgg tataggcata
    12181 tattcatcac aatattatgg aagcaaggaa atacctaaaa ttcattcaat gatgggaata
    12241 ggctttactt ttggcgtatc atttgcagtt cttctaacta tactgatttc cttatttcct
    12301 gatgatactg tcagaatatt cataaaagac gaatctgttg taagcattgg aactgaatac
    12361 ataaaaatag ctgtcttatc ttttatacct gcatctattt ctatggtttt tcaaatcaac
    12421 ttaagaagcg ttagaatgac caagattccc atgatgacaa gccttgttgc tgcaatattc
    12481 aatggtttac ttaattatgt actcatattc ggaaaacttg ggtttcctgc cttgggcgtc
    12541 aaaggagctg caattgctac tgtaactgct agattcattg aattagttat ggtattgcta
    12601 tttgtatttt ttaataaaaa ttgtcctttg aaaggtacaa ttaaaagtta tttttcatat
    12661 accctgccaa tgatggttac tgtttttaga agaagcctcc cggttgttct tcaagaaggt
    12721 ttatgggcaa taggaacttc tgtatacttt attgcatatg gaaatttgag ttcaacttca
    12781 gttgctgctg ctcagatatc cttaacttct cttgatatat acatatcgct tttcataggt
    12841 ttaggtaatg gagctggtgt aatggtagga aatcttcttg gagaaaaaca gatagagaaa
    12901 gcacaaagat atagcaacaa gataatttta taccaaataa tattatccat cccctttgca
    12961 atactgtttt ctttttcagg tatatggatt accagatttt tgaatttttc cgttgcaggt
    13021 aatcaggcaa ctttgtatac aatatatgtg ctcgctgcct atcttcctat taaatgtatg
    13081 aattttctat tcatagtcgg tattttacgt ggcggaggtg ataccagatt ctgcatgata
    13141 tcagagacag cttcagtatg gcttatagga gtgcctttag cttttatatc agttctagtt
    13201 ctcaaattac ctatttattg gtgtgtagca cttgtaaatc ttgaagaaat aactaaactt
    13261 atattatgct ctgtacgtta caagagtaaa aaatggataa atgtccttgt ggactgaatg
    13321 aggtattatg tataaactaa ttgcaacaga tatggatgga acattattga atgatgaagg
    13381 aaatctgcct tcaaatactt ttaatgcagc tcgtttttta gaagataatg gcattaagct
    13441 tgtattatgt acaggaagac cgttacttgg actaatcaag atttatgatt cactgaacct
    13501 gacctctcca atagtagcaa ataatggtgc atgcgcttat cttgataaat cgggtccttt
    13561 gattatgaat cagactatga gtattgaaga tggattagca gtaattaaat tgaatgaaat
    13621 ttttaactgc acaatatgta tgtggcacaa ccagatcttg tatgtcagtg aatttaataa
    13681 atatgcatat gattatgcaa gcataaccgg agttactccc ctgcatcttt caaaaattga
    13741 tttgaaaaaa gaaaagatag gacccacaaa atttgttttc agagacacac tagagaagat
    13801 tacatcaatc aaagaaagtg ctgcagaact ttcaccacct agtgttacag cagttaccac
    13861 tcggcctcat tttttggaat ttttcagcag ttcaatatct aaagcagctg cattagaaaa
    13921 aataggtcag acactgaaca tatcaaaaga tgaaatgatt gcagctggtg acgggttcaa
    13981 tgacatcgaa atgatggatt atgtcggact tgctgtggct gttcagaatg cagctgatga
    14041 agtaaaaaag catgctgatg ttatcggatg cagcaataat gaaggaatta ttgattacat
    14101 agttaacact ataattaaaa aatgacggta ttaaccgtca tttcttttta ctaattcaaa
    14161 attgatttta tgaaatattt tatgaaagtt tgaatgtctt aatctcataa ggcttgatat
    14221 taaactttag ggtgttattt tcaaactcaa cattacaaat atcattttcc ataaggtcgc
    14281 attcggttat cttagaaaca tcaaatccaa aatcaatagt gcaatcagta cgtctgttga
    14341 aacattcata aactcttaat acaagatcat cggtatcttc tgcttttttc aatgtatcaa
    14401 caattacatt ctcgtcattg catgtaataa atgatttgta gttctcaaca tttcctgttc
    14461 ccttttcaac ataatacgca tatggcctgt aattaagttt atatgccata tctattgtgt
    14521 gagctttttt aaagtcacct gtatgcggat acagagaata tgtaaattca tgaagacctc
    14581 tgtcagcatc tggataaggt tcgattgcag attttagaag tgtaagcctt agtactgatc
    14641 cttttatatc atgaccatat ttacagtcgt ttagcaaact tacaccatag tcatcttctg
    14701 aaaggtcagc ccatttatga gcacatactt caaatcttgc ataatcccaa ctggtattcc
    14761 aatgagtagg tctcttcaca ttaccaaatt gtatatcata tgcagcttca cttgcatgaa
    14821 catctactgg gaatgcggct tttatgagaa tatcatcatg attgcagtca ataatagttt
    14881 tgaaatccag tttctttgaa gatttttcaa gatatacata ttgtgtaatg gttgtttcca
    14941 aaaactttct atcaatcctt atgcactttc taataggacc atcttcaacc aattcagtgt
    15001 aagtcacatt atcaatatca tatggtttct cgttatagta aatgtttata tcccaagcct
    15061 gatatttaat tggtttgtct tcatatgctg ttatccggtt tgcagtctga ccatcaagaa
    15121 taacttctct attagctgtt ttatcgtata atgatgaaat attcatattt tcatcgaatt
    15181 ttactttcaa ataagcattt tccatcatat tatctgttgc tttaaagaaa ttatcagaaa
    15241 gatccacatc atcagatgaa agtttgaaca ctgtataacc taaaggacat agacgggctt
    15301 caaatatgaa atatttctta ttatcttcat taaccatctg tccaggtatc atattattaa
    15361 cagaatcata tacacaagta taatccttat cggtcctaat tctaattgta tcgacacgct
    15421 taaaggccag ctggttaaat acaacacaga gctcttttcc tttaactgtt ttagaaaaag
    15481 catgaagagc actggaaaga agttcttttc catctttcat taccctggca taatctatat
    15541 cagaatcatc ataaacttct tttatagaag aacctggaat aatatcatgg aactgattga
    15601 gaagaacgat gtcccagata tcataaagct ttttcttatc ataagtatcc agtccaagtt
    15661 tatcagcaat tgcactgaaa acttcagtgt ctcgaagcag gatttcagat tttctattat
    15721 tctttttatt tttagcaaca gaagtatatg taccacgatg gtactccaga tacagttcac
    15781 caacccattt tggaagtctc ttatcctctt taacttcttc atacaatctg tcaaagtagt
    15841 caagagccct tacattttcg atttttggca ttccttttat tcctttagaa aacctgttgc
    15901 actgttctat atggtccctg cttggtccac ctccgccgtc accaagacca aatgtcatta
    15961 atggactctt tgaaaggctc ttatccctat aattcttcca tgtccccata acattttctg
    16021 cattcattct agcgttatag tttactctag gcttcccaaa ctcatctgga ccgctacctc
    16081 caactataaa gttagtaaat acttcagaac catcaatccc ttcccacata aaagtattgt
    16141 atggtattac attgtattca ttccaggata tttttgatgt ggagaaatag tctacaccgg
    16201 atttcttcag aatttgcggc aaagctgcac tgtatccaaa aacatcagga agccataata
    16261 gcctgctgtc tttattaaac atctttttga tatatctctt gccatgcatt atctgtctaa
    16321 caaaagattc tccgcttaca agattacagt ctgattcaag ccacattgca ccttctgctt
    16381 cccactggcc attttcaact gcttctttta ttttgtctat catttcagga taatcttcct
    16441 caagatattt atataactgg ggttggcttg cgataaatct gaaactgtca taatcattca
    16501 tattcttaag ctgagttgaa aaagttctta cagttttttg tcttgtttct ttaagtggcc
    16561 atctccaggc tacatcaata tgagtatgac ctatagcact aatcataact ttagaggtgt
    16621 catcatcaca gtaattattg tagtattggt ctataagata attatcagct ttattaacag
    16681 aatcataata ttcttctgaa tatggatttc ttagatcaac catatttatt gcatttataa
    16741 gatacttagt ggtatccttc cttatagcct cgctttcaat aaattgaata gattcggcag
    16801 ggcacaaaat atcgtaatat aacttttcgg tcggcttgtc tagagagcat gtttttaaag
    16861 acatctgaac aggacctttt ttcatacctg aaaattcaaa aatgaagcag tcatactcat
    16921 catatgcttt tgcacaatca gtaattatac attcatgatg gccataatca aaggcctgta
    16981 atggctctcc atttacaaaa agaagaaagt ttggatttct atgttcattc cactcatatc
    17041 caaatcctgt tatcattttc agtactaatg gtaatttgtc atacttcgga ctgaccttta
    17101 atttaaactt gaaaatatgg tatttatttc cctcattttc ataaatagta ccaggttcgt
    17161 actgactcat tttagaaata tcagtatctt ttaataattt cagattaatc tttccctcaa
    17221 catcagctat acggatatcg tttacatcag ctaaatcagt aaatctcatc gtattcatgt
    17281 cgttaagaaa ttttcttata tgctgctgat caaaatacat atggtccttc ctttctactt
    17341 cataatattt tcaaatttat catactctgt tatatctata attcgtgatg attcatctaa
    17401 tattgcgttg ttctcatatg ctgacatcat atcttcatac aatcctttaa cataaacaag
    17461 ttttgttgta ttttcttctg catcaagata tcccaatcta tcgcttgata tctgagaaat
    17521 attataatac tggctaatag cattgatagt tttcaattgt ggaattgcag cttcaatagg
    17581 acagtaatta acatcatctc cagttaaagt tctcatgcaa tcaaatactt ttttcatttc
    17641 acaatcaaag gggttaccat atttt
//
LOCUS       DAOSXM010000041        17465 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2111
            NODE_2062_length_17465_cov_10.002585, whole genome shotgun
            sequence.
ACCESSION   DAOSXM010000041 DAOSXM010000000
VERSION     DAOSXM010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864178
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 17465)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17465)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:24:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,261
            CDSs (total)                      :: 2,229
            Genes (coding)                    :: 2,218
            CDSs (with protein)               :: 2,218
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 3 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17465
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2062_length_17465_cov_10.002585"
                     /isolate="AMR_MDS_2111"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            163..312
                     /gene="rpmG"
                     /locus_tag="PLQ68_06520"
     CDS             163..312
                     /gene="rpmG"
                     /locus_tag="PLQ68_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004402360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HXK72342.1"
                     /translation="MRDKIILACTECKQRNYSTKKNKKNTPDRLELSKYCKFCKKHTL
                     HRETK"
     gene            326..538
                     /gene="secE"
                     /locus_tag="PLQ68_06525"
     CDS             326..538
                     /gene="secE"
                     /locus_tag="PLQ68_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014253987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HXK72343.1"
                     /translation="MAEKEKNVKKSKFVGFFKGIAQELKKVTWPTGKQLFKSTVTVLV
                     VCAIVAVIISLFDWGLSSLIKLLVNG"
     gene            555..1118
                     /gene="nusG"
                     /locus_tag="PLQ68_06530"
     CDS             555..1118
                     /gene="nusG"
                     /locus_tag="PLQ68_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015310883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HXK72344.1"
                     /translation="MDENLNTDVTEKRENQNLAKWYVVHTYSGYENKVKADLEKIVEN
                     REMQEYIQDIVVPLEEVIEEKNGKRKISFKKIFPGYVIVKMVMNDQSWYVVRNTRGVT
                     GFVGPTAKPVPLTEDEVIQMRLEGMETVIDFNTGDNIRVIEGPFENFVGIIKEVDKDK
                     RKVKVHISIFGRETPVELDYDQIQIIL"
     gene            1152..1577
                     /gene="rplK"
                     /locus_tag="PLQ68_06535"
     CDS             1152..1577
                     /gene="rplK"
                     /locus_tag="PLQ68_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HXK72345.1"
                     /translation="MAKKIIGYVKLQLPAGKATPAPPVGPALGQHGVNIMGFCKEFNE
                     RTRNDTGLIIPIVLTVYADRSFSFITKTPPAAVLLKKACNIESGSATPNSAKVATVSK
                     ADLRKIAEQKMQDLNASDVESAMSMVAGTARSMGIKVEE"
     gene            1593..2288
                     /gene="rplA"
                     /locus_tag="PLQ68_06540"
     CDS             1593..2288
                     /gene="rplA"
                     /locus_tag="PLQ68_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HXK72346.1"
                     /translation="MKRSKNYRASLELFDKATSYDPSEAVKLVKSTAKAKFDETIELH
                     IKLGVDSRHAEQQVRGAVVLPHGTGKNVRVLVFAKGANADAAEKAGADYVGADELADK
                     IKNENWFEFDVAIASPDMMGVVGKIGRILGPKGLMPNPKAGTVTPDVAKAVTEAKAGK
                     IEYRLDKTNIIHCPIGKASFDEEKLLENYKTLMNAVIKAKPAASKGQYLKNVTLASTM
                     GPGIRVNTQKALD"
     misc_feature    2344..2473
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            2484..2993
                     /gene="rplJ"
                     /locus_tag="PLQ68_06545"
     CDS             2484..2993
                     /gene="rplJ"
                     /locus_tag="PLQ68_06545"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HXK72347.1"
                     /translation="MASEKILEVKKQQMAELREKLLTMGSVILADYRGLTVAQDTAMR
                     SEMRKANIEYKVIKNRLLKKAVEGTKYEDLMPHLEGPTSVAMSEDEISAAKIMSKYAN
                     MKEYGKFELKAGVIGDQIVDVNGIEAYAKIPGKNELIAMMLGGMKAPITSLALVLKAI
                     MEKKEEPAA"
     gene            3024..3407
                     /gene="rplL"
                     /locus_tag="PLQ68_06550"
     CDS             3024..3407
                     /gene="rplL"
                     /locus_tag="PLQ68_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011917713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HXK72348.1"
                     /translation="MMSEKITKLIEEIKTLTVLELADMVKALEEEFGVVAAAPVAAAG
                     AAAGPAAVEEAEEQTEFNVLLKEVGAEKIKVIKVCREATGLGLKEAKDLVDNAPSTIK
                     EAISKQAAEELVAKFKEVGATAEIK"
     gene            3594..4859
                     /locus_tag="PLQ68_06555"
     CDS             3594..4859
                     /locus_tag="PLQ68_06555"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HXK72349.1"
                     /translation="MKEYLSEMIDNLGKLIAIKSVKGDPEPNAPYGIETLKALEFMLE
                     LGKKYGMKAVNVDGRVGYLEYGEGEDMIGILLHLDVVPEQDGWLSDPYTLTQKDEYLY
                     GRGVSDDKGPAIAAFYAVIDLIRKNEIKGYRVRLIFGLDEECGSSCMAHYVKTQELPT
                     MGFVPDADFPVIYAEKGILTVCIEGEGSKNIHLKAGDRPNVVASRCVVRYKGEETVYE
                     GIPAHGAAPFNGLNAIKVAIENSTAIKGHPIVEFFRDCLSDKHFGEGLNISFNDESGK
                     LTVNPGIMSISKDNSYCVLNIRYPVTADYEEITENIKRVCKKYGLYISSVSDLPPLYV
                     QKDSVLVSTLLKVYREMTNDLRNPIAIGGGTYARTMPNLVAFGMNMPGDPEKAHQANE
                     CLKKQRLYEGAAIYRESIKRLGETLVNQK"
     gene            complement(4846..6624)
                     /locus_tag="PLQ68_06560"
     CDS             complement(4846..6624)
                     /locus_tag="PLQ68_06560"
                     /inference="COORDINATES: protein motif:HMM:NF021355.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller domain-containing protein"
                     /protein_id="HXK72350.1"
                     /translation="MKKIISLLVILILLLTGCNSVIHTEQPTPTSTTQNLKQIASMSQ
                     LKTLLEANKDNLVSRWFRGITAPTMYKNSEDAESSVESAQGSSDYSQTNTQVSGVDEA
                     DTVKTDGLYIYQINDFGKVHIVSVDNDGQLNLVNQIIDFDEDFHPRELYVDDSFLIVI
                     GSSYNNDGSYTTAYVYDKTDVTNLSLLKNIMIKGYYVSSRKIGTSLYLISNYSPSYYY
                     LKDTELLPCYIEDGITTYQTPNEIYYFDDVDYSSYLILGGIDLSDLSKDVNLNTYLGS
                     GQTIYMSQENLYVTYTTYQYNRTVNILAENSSDYKQTSVIHKFSAESAVLNYVATAEV
                     EGYPINQFAMDEHNGFFRIAVTATKDGESVNNVHIFDSELIQTGTISNMAPGERIYSV
                     RFNGDIGYMVTFVSVDPLFVMDLSDPYNPKVDGELKIPGYSTYMHMYSDTLILGFGMD
                     TDSSEGRVLNQGIKIAMFDVSDRTNPTQLFMTVIGGRGTWSPLNNDHKAFMINRERNI
                     FTFPAQIAGNNYEMKAQGLVVCRIDIENNQFEVLDILSQDYFTNWNNYVQRGLYINNI
                     LLTISSNAIYSYNFDSLELIDNVIFD"
     gene            6793..7407
                     /locus_tag="PLQ68_06565"
     CDS             6793..7407
                     /locus_tag="PLQ68_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006313298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HXK72351.1"
                     /translation="MKTKLNLNIEILLNNLNKNNMLSVFVEDREHARLEVERLIKKGS
                     SIAFGGSVTLNQCEITQMIEKGDYVICDRSDKAAFFNSDYMLTSVNAITQDGILYNVD
                     GASNRIAGICYGPKKVVVVAGINKLVKDIDHAVLRVKHISAPKNCVRLNRNTPCARTG
                     KCISDDINKGCDSVDRICCNYLISAKQREKDRISVIIVNEELGF"
     gene            7411..7842
                     /locus_tag="PLQ68_06570"
     CDS             7411..7842
                     /locus_tag="PLQ68_06570"
                     /inference="COORDINATES: protein motif:HMM:NF022281.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2752 domain-containing protein"
                     /protein_id="HXK72352.1"
                     /translation="MWKNALNKLVSDLKKVWIPITACILYVVIMTILVGEVCPSKLIF
                     GIPCAGCGMVRAFILFITFQFREAFAMNPGIFIVAAAFLIFVILRYFTHVDIKWIKIL
                     LIITLLSLIIIFVIRLITSFGTEPLVIYDKAFIFWIMKQFS"
     gene            7890..8297
                     /locus_tag="PLQ68_06575"
     CDS             7890..8297
                     /locus_tag="PLQ68_06575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72353.1"
                     /translation="MTEINDNVTNEKQEIPVNTEKTYIVNENPAENQQQPHITNQYVP
                     KPDVSPTVSILDWILTIIVMAIPCVNVIMLFVYAFSGDKESKKNYFKARLILVAASII
                     ATIIFFVVMFAILGPRIPEFIEEFENLRRNYGF"
     gene            complement(8469..10199)
                     /locus_tag="PLQ68_06580"
     CDS             complement(8469..10199)
                     /locus_tag="PLQ68_06580"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HXK72354.1"
                     /translation="MKKMKLLSLVLCLILVCSFIFTACDNKPTETATETPTESATETA
                     TETPTETSTEPEVVEKPKQLDITTLYYYGDATENSSQKLAFKNFISRHYGIQFNLNTP
                     ARDTYIETINLQAVSGDLTGMVRLFSGTDGIRWYQEGVVLAIDQYLADNETWVNIIPE
                     NWKETFSYDGTVYGLAFGGDGIPSWFSRTMRGDWLDAVSMAKPETIEQFYEVSKAFTY
                     NDPDGNTVNDTWGFASRNIWLMQDIFKAYGCTPNWEADLVPVWNPRDDIWEDSVIKPV
                     MADCALFLRKCYSEGLVHPELFSMSSTNVRNLISNGQAGSCFYWDTWLISWENSVKQT
                     VGSQAYMVAVGALTGNVTQKINCWKTDIGAPYVLMANTEQPKEVVNWFITLCYGTAED
                     FFTFRYGINQESIDAKTGFFLDGKTVYQLHYQADDQNAVKSTGTPAIIGGHPGYALDI
                     GTYGYKSAYNSGNAQWDSDQITKSAADLERRYEVFNEYNDGRLYVLTGDYEQPTNEEY
                     TMVISEWAGAGMKYVSSVMVDGVEPNVALKAYLEVVMAFNPQVTLDFMNSKLGKASTQ
                     NYNELWGSLN"
     gene            10602..13016
                     /gene="leuS"
                     /locus_tag="PLQ68_06585"
     CDS             10602..13016
                     /gene="leuS"
                     /locus_tag="PLQ68_06585"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HXK72355.1"
                     /translation="MIVMEYNPKEIESKWQKKWDELKCFKAKDDLSLPKYYALVEFPY
                     PSGEGLHVGHPRPYTALDIVARKRRLQGYNVLFPMGWDAFGLPTENYAMKNKMHPAAV
                     TKKNIARFKSQLKSIGYSFDWDREINTTDPNYYKWTQWIFLQLFNHGLAYKKNMSINY
                     CTSCKVGLANEEVVDGKCERCGAEVIKKMKNQWMLKITEYADKLIEGLSEVDYLESIK
                     SQQINWIGKSTGAEVDFMLTGIEEPLTVYTTRPDTLYGATYMVISPEHPYIEKYKSLI
                     TNLDEIKQYQIESSKKSDFERAEIAKDKTGVEIKGIRAINPLTDKEIPVWISDYVLMS
                     YGTGAIMAVPGHDQRDYEFAKKFNLPIIEVVEGGDLEKEAFTDIEKGIMVNSGIINGL
                     EVKDAKKKITEYIESKGIGKTKDNFKLRDWVFSRQRYWGEPIPLVYCEKCGWVPLDES
                     ELPLLLPDLDSYEPAEDGESPLSKMTDWVNTTCPKCHSSAKRETDTMPNWAGSSWYFL
                     RYLDPNNDICFADKEKINYWMPVDWYNGGMEHVTLHLLYSRFWHKFLYDIGYVNTPEP
                     YSKRTAHGMILGENNEKMSKSRGNVVNPDKVIDNYGADTLRMYEMFIGDFEKSAPWSE
                     NGVKGCRRFIDRAYKMMDFMNDSDDYSKDLLTIMHKTIKKVTNDFETLKFNTAIAQLM
                     AFSNAVYLKGSITKKEMQSFLILLNPVSPHITEEMWTLCGFDGYIHEKSWPEYDEDKT
                     IDDEIEIVVQVNGKIKAKIMISSDMSKQEMESLVETEDAIIEVLEGKKPKKIIAVPKR
                     LINIVI"
     gene            13025..14083
                     /locus_tag="PLQ68_06590"
     CDS             13025..14083
                     /locus_tag="PLQ68_06590"
                     /inference="COORDINATES: protein motif:HMM:NF008519.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HXK72356.1"
                     /translation="MDLYDIAVIGAGPCGSVLSMLLAKQGYKIAVIDKRNLDCLPPTR
                     SKACGGLLSPDAQRVLASLELNLPNNVLVDPQLFSVVTIDLETGLQRHYQRFYLNMDR
                     EKFDRYLVSLIPDNVVRLFSTIVKDISYDSSGYQIFMDDNKSLKTKIIIGCDGAGSIV
                     RKKMFNDLKMNVKNYYAIQHVYEYSKYNSNYYAIFDKNLTDYSGWAFPKNGTFSIGMA
                     FKQESEGNSFFDILIEKLEKKGMKLGKLIKKEGALLLRPCGIPKVYAKKDNTAFLAGE
                     AAGYISPSSAEGFSFAFNSACALAEAFKKGGNISKNYCKLTLKMRIKLILKIIKARLM
                     YNQYVRKIIMALKINAIR"
     gene            14096..>14658
                     /locus_tag="PLQ68_06595"
     CDS             14096..>14658
                     /locus_tag="PLQ68_06595"
                     /inference="COORDINATES: protein motif:HMM:NF024740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HXK72357.1"
                     /translation="MGLEKIKCFVLDMDGTIYLGNSLFPYTKHFLEVAEKCGIDIWYY
                     TNNSSKNAKFYVEKLKRMSIDCESHKMLISNQVIIQYILKNHRNCKVYVVGTEYLVND
                     FVDAGINVTDKNCDMVVMGFDTTLTYEKLVKACDFVRNGATLYGVNMDYNCPTETGFI
                     PDCGSICKLVLASTGVQAEFFGKPSHHT"
     assembly_gap    14659..14758
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(14902..16215)
                     /locus_tag="PLQ68_06600"
     CDS             complement(14902..16215)
                     /locus_tag="PLQ68_06600"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HXK72358.1"
                     /translation="MRKGISSILVILITISLFSISIQGQAQTVINPDSCLSYLSMDFE
                     TGDVLYSYNSDMKIYPASTTKLLTALVAVENCPLNKIFVTSKNATRFLEDGSGELELL
                     PDETMDFYSLLHGLLIRSFNDVALVIAENVAGSEEEFMNMMNQKAQELGAVNSHFITP
                     HGLHDDEHYTTALDLAIIARAAFSNFTISTICAKKEYSLPATNLRQNFDLIKNTNMIL
                     GTNTGYDFTVTAGKTGFTSKAQNVLVALSSDYSGREVITVITGVKDRVEAAEITLELM
                     AHSYEDFGIQCIAQAGQIIDNHEGINIANAMRLEHLVPYDSQSWTLDKTFDYRSDIIY
                     SQVDIGDIMGEVTYSYNGNYIGKSYIVAASSLKPVKNDIDITQYSEDIDVETTKVEYD
                     KPFVPEFAISLGISVLLLVLIVITFYKTRKHENKNEDKEISDQNE"
     gene            16358..16957
                     /locus_tag="PLQ68_06605"
     CDS             16358..16957
                     /locus_tag="PLQ68_06605"
                     /inference="COORDINATES: protein motif:HMM:NF016787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HXK72359.1"
                     /translation="MKKHIPTFLIFGILLCIMIIMIIRAVNKKIVFGNDDTSNTYEQM
                     EEKPETLLICIKGEIKNPGIYEIEKGSILNDLVILAGGFTENAGKDINLVYELNENLT
                     IYIRSEEESGGMDIIDDKDIIFQSDDEKVLFENKVDINTSDIQTLCLLPGIGEKTAEE
                     IIRYRDSIGIFETIEDIMNVTGIKNAKFEKIKDYITIGN"
     gene            16999..17081
                     /locus_tag="PLQ68_06610"
     tRNA            16999..17081
                     /locus_tag="PLQ68_06610"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17033..17035,aa:Leu,seq:tag)
     gene            17168..17422
                     /locus_tag="PLQ68_06615"
     CDS             17168..17422
                     /locus_tag="PLQ68_06615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72360.1"
                     /translation="MKRKRLDRDIWTSIVEKEYFQENINEPDFSGILSVLYLKKVFPD
                     SVWNANHESFAVASSGMKWLQLLSSEKNYLLTAMISDKMK"
BASE COUNT         6109 a         2570 c         3324 g         5362 t
ORIGIN      
        1 gggatttctc ttttatttta tcaatcccca aacaagttta cactatcaat aaaaaaaata
       61 tattgacaca atatatatgg acatgataag atatctcttg ctattaggag gctctttttt
      121 tttgtgaaaa agtcttcaat aagataaagg caggaagaga aaatgagaga caagattata
      181 cttgcgtgta cagagtgcaa acagagaaat tactctacaa aaaagaacaa gaagaatact
      241 ccagacagac ttgaattaag taaatattgc aagttctgca agaagcatac tcttcataga
      301 gaaacgaaat aatagggtga cgacgatggc tgaaaaagaa aaaaatgtaa agaaatcgaa
      361 attcgtagga ttcttcaaag gaatcgcaca agagcttaag aaggttacat ggcctacagg
      421 caaacagctt tttaaaagca cggtaacggt tttggtggta tgtgccattg tcgctgttat
      481 tatcagcttg tttgattggg gtttatcttc tctgatcaaa ttgcttgtaa acggataaac
      541 gggagaatat ttcaatggat gagaatttaa atactgatgt aaccgaaaaa agggaaaatc
      601 agaatctggc taaatggtat gttgtgcata cctattctgg ctatgaaaac aaggttaaag
      661 cagatcttga aaaaatagtc gagaacaggg aaatgcagga atacatacag gatatagtcg
      721 ttccgttgga agaagttatt gaagaaaaga acggaaaaag aaaaatatcc tttaagaaga
      781 ttttccccgg ttatgttatt gtaaaaatgg ttatgaatga ccagtcatgg tatgtagtaa
      841 gaaataccag gggtgttaca ggatttgtag gtcctacagc aaaacctgtt cctcttactg
      901 aagacgaagt cattcagatg agactggaag gtatggaaac tgttattgac tttaatacag
      961 gagacaatat cagagttatt gaaggacctt tcgaaaactt tgtcggaatc attaaagaag
     1021 ttgataaaga caaaagaaaa gttaaagttc atatctccat atttggcagg gaaacacctg
     1081 ttgaactgga ttatgatcag atacagatta ttctttaagc taaaaagaaa aaacattggg
     1141 aggtggattg aatggcaaag aagattatcg gatatgtaaa actgcaattg cccgcaggaa
     1201 aagcaacgcc agcaccgccg gttggaccag cactaggaca gcacggtgta aacattatgg
     1261 ggttctgtaa ggaattcaat gagagaacaa gaaatgacac aggactgata ataccaatag
     1321 tattaacagt atatgctgac agatcattct catttattac taagactcct ccagccgcag
     1381 tattgcttaa aaaggcatgt aacattgaaa gtggttcagc aacgcctaat tcggctaaag
     1441 ttgccacagt ttcaaaggct gatttgagaa aaatcgctga acagaaaatg caagatctca
     1501 acgcatcaga tgttgaatct gcaatgagca tggttgccgg tactgcacgc agtatgggaa
     1561 tcaaggttga ggaataatag gaggaagcca taatgaaaag aagtaaaaat tatagagcaa
     1621 gtcttgaact atttgacaaa gcaacctcat atgatccttc agaagctgta aaactggtta
     1681 agtcaactgc aaaagcaaaa tttgacgaaa caattgagct gcacataaag ttaggtgttg
     1741 actcaagaca tgctgaacag caggtcagag gagcagttgt tttaccacat ggcacaggaa
     1801 aaaatgtcag agttttggta tttgctaaag gtgcaaatgc tgatgcagct gaaaaagccg
     1861 gagctgatta tgtaggtgct gatgagttag cagataaaat caagaatgaa aactggtttg
     1921 aatttgatgt agctatagca agtcctgaca tgatgggtgt agttggtaaa ataggtcgta
     1981 ttttaggacc taagggactt atgcctaacc caaaagctgg aacagttaca cctgatgtag
     2041 caaaagctgt aactgaagct aaagcaggta aaattgagta cagactggat aaaactaata
     2101 ttatccattg tcctattggg aaagcttcat ttgacgaaga aaagctttta gaaaattaca
     2161 agacattaat gaatgcagta ataaaagcaa aacctgctgc atcaaaaggt cagtatttga
     2221 agaatgtaac attagcttcc actatggggc ctggtataag agtaaatact caaaaagctc
     2281 ttgactagtt aagacttata gattaatatc taatagatat tattagataa tatcataaga
     2341 aaagtaaata aagtaaatct gccgcagaca gcaggtgctg gtaaagcgta agtcctgccg
     2401 aggaagatac aagtaattga ttaaattcaa atcctcgtat gtttgcagct tgcgaggatt
     2461 ttttataagg aggtaacgaa aaaatggcta gtgaaaagat attagaagtt aaaaagcaac
     2521 aaatggctga actaagagaa aaacttttaa ctatgggttc tgtaatttta gcagactaca
     2581 gaggacttac agttgctcag gatacagcta tgcgttctga aatgagaaaa gctaatatcg
     2641 agtataaggt catcaagaac aggcttctca aaaaagcagt tgaaggtaca aaatacgagg
     2701 atttaatgcc tcatttggaa ggacctacat cagttgctat gagcgaagat gaaatttcag
     2761 cagcaaaaat aatgtcaaaa tatgcaaata tgaaagagta tggcaaattt gagttaaaag
     2821 ctggtgttat aggtgatcag atagttgatg ttaatggtat cgaagcatat gctaagattc
     2881 ctggtaagaa tgaacttatt gcaatgatgt tgggtggtat gaaagctcct atcacttcat
     2941 tggctttagt tcttaaggca ataatggaaa agaaggaaga acccgctgca taagcgataa
     3001 attacactaa ttggaggata agaatcatga gtgaaaagat tacaaaatta atagaagaaa
     3061 ttaaaacatt aacagtatta gaacttgctg atatggtaaa agctctcgaa gaagagtttg
     3121 gcgtagtagc agcagctccc gtagcagcag caggtgcagc agcaggtcct gcagcagtag
     3181 aagaagcaga agagcaaaca gaattcaacg ttttacttaa agaagtaggc gcagaaaaaa
     3241 tcaaagttat taaagtttgc agagaagcaa caggcttagg acttaaagaa gcaaaagatc
     3301 ttgttgataa cgctccaagc acaattaaag aagctatttc aaaacaagct gcagaagaac
     3361 tcgttgctaa attcaaagaa gtaggcgcaa cagctgaaat aaagtaaatt aagaagataa
     3421 aaaaatccga gcgcaaagga acccctttat atggggtttc tttttgtctg tttttggtat
     3481 aaacttgttt acgatatgtt atataacgaa ttatttttaa tgaaaataag tatatagatt
     3541 gatataggat aaaataatac taatagttaa aacagaaaga gaaaggtata gacatgaaag
     3601 aatatctaag tgaaatgatt gacaatttag gaaaattgat agctataaaa agtgtaaaag
     3661 gtgacccgga accaaatgct ccatatggta ttgagacttt aaaagctttg gagtttatgc
     3721 ttgaacttgg gaaaaaatat ggtatgaaag ctgtaaatgt agatggaaga gttggttatt
     3781 tagaatatgg tgagggcgaa gatatgatag gcatactgct gcatcttgat gttgttcctg
     3841 agcaggatgg ctggttaagt gatccttata cccttacaca aaaagatgag tatctttatg
     3901 gcagaggtgt atctgatgat aaaggaccag caatagcggc attctatgca gtcatagact
     3961 tgataagaaa gaatgaaatt aaaggataca gagttagatt gatattcggt cttgatgaag
     4021 aatgtggttc ttcctgtatg gctcattatg taaagactca ggaattacca acaatggggt
     4081 ttgtgccaga tgctgatttt cctgtaattt atgctgaaaa ggggatactt acagtatgta
     4141 ttgaaggaga aggaagcaag aatatacact taaaagcagg tgataggcca aatgtagtag
     4201 caagcagatg tgtagtcaga tataagggag aagagacagt ttatgaaggc ataccagctc
     4261 atggtgcagc accttttaat ggactcaatg caataaaagt tgcaattgaa aacagtactg
     4321 cgataaaagg acatccaatt gttgaattct ttagagactg tctttcagac aagcatttcg
     4381 gtgaggggct taatataagc tttaatgatg aaagtggtaa gttaactgtt aatcctggaa
     4441 taatgtctat aagtaaagac aattcgtatt gtgtccttaa cataaggtat ccagtaaccg
     4501 ctgattatga ggaaattact gagaatatta agagagtgtg taagaaatac ggtctttata
     4561 taagctcagt atcagacctt cctccgctat atgtgcagaa agactctgtt cttgtttcca
     4621 cattacttaa ggtttatagg gaaatgacaa atgacctgag aaatccaata gcaataggtg
     4681 gtggaactta cgcaaggaca atgcctaatc ttgtagcttt tggtatgaat atgccaggtg
     4741 atcctgaaaa ggcgcatcag gcgaacgaat gcttgaaaaa gcagcgccta tacgaaggcg
     4801 ccgcaattta cagggagagt attaaaagat tgggtgaaac gcttgtcaat caaaaataac
     4861 gttatctata agctctaaac tatcaaagtt gtaagagtaa attgcatttg atgaaatggt
     4921 aagaagaatg ttatttatat ataatcctct ttgaacatag ttgttccagt ttgtgaagta
     4981 gtcttgagat aatatatcaa gaacctcgaa ctggttattt tcaatgtcaa ttctacacac
     5041 tacaagccct tgagctttca tttcgtaatt attacctgca atctgagcag ggaaagtgaa
     5101 tatgtttctt tctctgttaa tcataaatgc tttgtgatca ttatttaaag gagaccatgt
     5161 tccacgacct cctattactg tcataaacag ctgagtgggg ttagttctat ctgatacatc
     5221 aaacatcgca attttgatgc cctgattaag aacacggcct tctgaggagt cagtatccat
     5281 tccaaatcca agtatcaaag tatcagaata catgtgcatg tatgtggaat atccaggaat
     5341 ttttagttcg ccatcaactt taggattgta tggatcacta aggtccatca caaacagagg
     5401 gtcaactgat acgaatgtta ccatataacc tatatcgcca ttgaatctga ctgaatatat
     5461 cctttctcca ggggccatat tggatattgt tcctgtctga atgagttctg aatcgaatat
     5521 atgaacatta tttacggatt ctccgtcctt ggtggctgtt acagctattc tgaaaaaccc
     5581 attatgctcg tccatagcaa actgatttat cggataccct tcaacttcag cagttgcaac
     5641 ataatttaga actgcagatt cagcactgaa cttatgaata actgaagttt gcttataatc
     5701 agaagagttt tctgcaagaa tgttaacagt tctgttatac tgatatgttg tatatgtaac
     5761 atataaattt tcctgggaca tatatattgt ttgaccactt ccaaggtatg tatttagatt
     5821 aacatctttc gaaagatctg ataaatcaat tccgccaagt attaagtatg acgaataatc
     5881 aacatcatca aaatagtata tttcatttgg tgtctgatat gtagttatgc catcttcaat
     5941 atagcatgga agaagttcag tatctttaag gtaataatat gatggcgaat agttgcttat
     6001 aagatacaat gatgttccga tttttcttga tgatacataa taacctttta tcattatgtt
     6061 tttaagcagt gacagatttg taacatccgt tttatcatat acatatgctg ttgtgtatga
     6121 gccatcatta ttgtatgaag aaccgataac gattaaaaat gaatcatcaa catacagttc
     6181 tcttggatga aaatcttcat caaaatcaat tatctgattt accaaattta attgcccatc
     6241 attgtcaaca gatactatat gaactttgcc aaaatcattt atctgataga tatataatcc
     6301 atctgtttta acagtatcag cttcatcaac acctgatacc tgagtgtttg tttgtgagta
     6361 atcagaacta ccttgagctg attcaactga agattcagca tcttcagaat tcttatacat
     6421 cgttggagct gtgattcctc tgaaccacct tgatactaga ttatccttat ttgcttcaag
     6481 aagtgttttc aattgtgaca tgctggctat ctgcttcaga ttttgggtag tactggtggg
     6541 tgtaggctgt tctgtatgta ttacagaatt acagcctgtt aataacaata ttagtatgac
     6601 tagaagtgat attatctttt tcattttatt tgccttcctt ttttccgatt acaattttta
     6661 gacgagcgtc ataatcaaaa gttccttagg catgtttcaa aggattattg tataattaag
     6721 ataattgaaa atttagcagt tcgtattcag tagaagagta ataaattggt aattagaaga
     6781 agggtaatag atatgaaaac aaaattaaat ttaaacatag agattttatt gaataatctt
     6841 aataaaaaca acatgctttc tgtttttgtt gaagatagag agcatgcaag acttgaagta
     6901 gaaagattga ttaaaaaagg ttcatctata gcttttggag gatctgtaac tttgaatcaa
     6961 tgcgaaatta cacagatgat agaaaaagga gattatgtaa tttgtgatcg gtcagataaa
     7021 gcagcatttt ttaattctga ttatatgctg actagtgtta atgcaataac acaggatggt
     7081 attctttaca atgttgacgg agcaagtaat aggatagccg gaatctgtta tggtccaaaa
     7141 aaagtagttg tcgttgcggg tataaataaa ttggtaaaag atattgatca cgcagtgtta
     7201 agggtaaagc atataagtgc tccaaagaac tgtgtaagat taaatagaaa tactccatgc
     7261 gcacgtactg gaaaatgcat ttcagatgat ataaataaag ggtgcgacag tgttgacagg
     7321 atttgctgta attatcttat aagtgcaaag cagagagaaa aggacagaat ttcagttatc
     7381 atagtaaatg aagaattagg tttttaatat atgtggaaaa acgccttaaa taagcttgtt
     7441 agcgatttaa aaaaagtatg gatacctata actgcatgta tattgtatgt agttataatg
     7501 actattcttg taggtgaggt ttgtccatca aaattaatat ttggtattcc atgtgcaggt
     7561 tgtggaatgg ttagggcatt tattttattt ataacttttc agtttagaga agcatttgca
     7621 atgaatccag gaatttttat agttgcagca gcttttttaa tatttgtaat acttagatat
     7681 tttactcatg tggacattaa atggattaag atacttttaa ttataacttt gttatctcta
     7741 attataatat ttgttataag gttaattaca tcttttggta cagaaccgct ggtaatatac
     7801 gataaagctt ttatattttg gataatgaag cagttttctt gacaatacat agattgataa
     7861 tataatttag ataaatgggg gatataatta tgacagaaat aaatgataat gtaacaaatg
     7921 aaaagcagga aattccggtc aatacagaaa aaacctatat agtaaacgaa aatcctgctg
     7981 aaaaccaaca gcagccacat attactaacc aatatgtacc taagcctgat gtatcgccga
     8041 cagtttcaat actcgactgg atattaacca ttattgtgat ggcaatacca tgtgttaacg
     8101 ttatcatgct ttttgtttat gcttttagtg gcgataaaga aagtaagaag aattatttta
     8161 aagcacgctt gattcttgta gctgcttcaa taatagctac aattattttc tttgtagtta
     8221 tgtttgcaat cttaggacca agaattcctg agtttataga ggagtttgaa aaccttagaa
     8281 ggaattatgg attttaataa atatttatta attcacgata ttaaaaatgt tagaaaaata
     8341 ctcaatgaaa aaaacctatt gactactaac taaaattaat ctaactttac gatatagatg
     8401 aaaatataat atttaaaaat ggtaaaaaaa gaacccggaa atttccgggt tctttaaact
     8461 taactaaatt agttcagact tccccaaagc tcattatagt tctgagttga agctttacca
     8521 agttttgagt tcatgaaatc taatgtaacc tgaggattaa atgccattac aacctcaaga
     8581 tatgctttaa gagcaacatt aggttcaaca ccatcaacca ttacacttga aacgtatttc
     8641 ataccagcgc ctgcccattc agaaataacc attgtatact cttcatttgt tggctgctcg
     8701 taatctccag taagaacata aagtcttccg tcattgtatt cgttgaatac ttcatatctt
     8761 ctttcaagat ctgctgcact ctttgttatc tggtctgaat cccattgtgc attacctgaa
     8821 ttataggctg atttataacc atatgtacct atatccaaag cataaccagg atgtccgccg
     8881 attatagcag gtgttcctgt gctctttaca gcgttttggt cgtcagcttg ataatgaagt
     8941 tgatatacag tttttccatc aaggaagaat cctgttttcg catcaataga ttcctgattg
     9001 ataccatacc tgaatgtgaa gaaatcttca gctgttccat aacagagtgt tatgaaccag
     9061 tttactactt ctttaggttg ttcggtattt gccataagaa catatggagc tccgatatca
     9121 gttttccaac aatttatttt ctgtgttaca ttacctgtta atgcaccgac agctaccata
     9181 tatgcttgag atcctactgt ctgtttaaca gaattttccc atgaaattaa ccaagtatcc
     9241 caatagaagc aggatcctgc ttggccgttt gatataagat ttctaacatt tgttgagctc
     9301 attgagaaca attctggatg aacaaggccc tcagaatagc attttcttaa gaaaagagca
     9361 caatctgcca ttacaggttt gataactgaa tcttcccaga tatcatctct tggattccat
     9421 actggaacaa ggtcagcttc ccagttagga gtacatccat atgctttgaa tatgtcctgc
     9481 ataagccaga tatttcttga agcgaatccc caagtatcat ttactgtatt tccatcagga
     9541 tcattatatg tgaatgcttt ggaaacttca tagaactgtt caattgtttc aggtttagcc
     9601 attgatacag cgtcaagcca gtcgcctctc attgttcttg agaaccatga aggtattccg
     9661 tctcctccaa atgcgagtcc ataaacggtt ccatcatatg agaaggtttc tttccaattc
     9721 tcaggtataa tattaaccca agtctcatta tctgcaagat actgatcaat tgctaatact
     9781 actccctctt gataccatct aattccatca gtacctgaga acagtcttac cataccggta
     9841 agatcacctg atacagcctg taggttgata gtttcaatgt aggtatctct agctggtgta
     9901 ttcaaattaa actgtattcc ataatgtctt gaaataaagt tcttgaatgc aagcttttga
     9961 gaagaattct cagtagcatc accataatag tacaaagtag tgatatctaa ctgttttggt
    10021 ttttctacta cttcaggttc agttgaagtt tcagtaggtg tttctgttgc tgtttctgtt
    10081 gctgactcag taggtgtttc tgttgctgtt tctgttggct tattatcaca agccgtgaaa
    10141 atgaaagaac acaccaatat gaggcatagc accaggctaa gcagtttcat ttttttcatt
    10201 attcttcctc ctttgaataa tattcaaaat ccatacctaa attatacact aacattttta
    10261 taagtaaagt aactaattaa acattttata aaaatatata ttattatatc cttttcttgt
    10321 aaatattcat taaatatgca ttaaaattca aaactatcaa aacatgtgca attggcatat
    10381 aatataaaat attagtataa attagacaat aatacgcaaa tataacaaat aatgtatgta
    10441 ttttagaatg tcaattaatg tatcgattta ttttatttag gagttagatt aaatagaaat
    10501 tcgttcatag ctttaccatt tatatataaa taaatacagc atttcttgac attgaatact
    10561 ttatgcaata aaatcgttga aagaatattt tgtaaacgga gatgatagtt atggaataca
    10621 atccaaagga aattgaatct aaatggcaga agaaatggga cgagttgaaa tgttttaaag
    10681 caaaagacga tttatcgcta ccaaagtatt atgcactagt tgagtttcct tatccctcag
    10741 gtgaaggttt acatgtagga catccaaggc catatacagc attggatatc gttgctagaa
    10801 aaagaaggct tcagggatat aatgtgttgt ttccaatggg gtgggatgcg tttggacttc
    10861 caactgaaaa ttatgcaatg aaaaataaga tgcatccagc agcagtaaca aaaaagaata
    10921 ttgctagatt caaatctcag cttaaatcaa taggttattc atttgactgg gacagagaga
    10981 ttaacacaac tgacccaaat tattataaat ggactcagtg gatttttctg cagttattta
    11041 accatggttt agcatataaa aagaatatga gcataaatta ctgcacatcc tgcaaagtag
    11101 gactggctaa tgaagaggtt gtagatggaa aatgcgaaag atgcggtgct gaagtaataa
    11161 agaagatgaa aaatcagtgg atgctgaaaa taacagagta tgctgataag cttattgaag
    11221 ggttatctga agtcgactac ttggaaagca taaaatcaca acagattaac tggattggca
    11281 aatcgacagg agctgaagtg gatttcatgc ttactggaat tgaagaacct cttacagtat
    11341 atactacaag gcctgatact ttgtatggag ctacatacat ggttatttca cctgagcatc
    11401 catatattga aaagtacaag tcattaataa caaatcttga tgaaattaag caatatcaga
    11461 tagagagctc aaaaaaaagt gattttgaac gtgctgaaat cgcaaaagat aagacaggcg
    11521 tagaaataaa aggaatcaga gctataaatc cgctgacaga taaagagata cctgtttgga
    11581 tttctgatta tgttttaatg tcatatggaa ccggagctat tatggctgta cctggacatg
    11641 accaaagaga ctatgaattt gctaaaaaat tcaaccttcc tataatcgag gtagttgagg
    11701 gaggcgattt agaaaaagaa gcctttactg atattgaaaa aggaattatg gttaattctg
    11761 gaataattaa cggcttggaa gttaaagatg caaaaaagaa gataactgaa tacatagaat
    11821 caaaaggaat aggtaagaca aaagataatt tcaaacttcg tgactgggta ttttccagac
    11881 aaaggtactg gggagagccc atacctttag tttattgtga aaaatgcgga tgggtacctc
    11941 tagatgagtc agaattaccg ttattgcttc ctgatcttga ttcatacgaa cctgcagaag
    12001 atggtgaatc accgctttca aaaatgactg actgggtaaa tactacatgc ccaaaatgtc
    12061 attcaagtgc aaagagagag acagatacaa tgcctaactg ggcaggttca tcatggtatt
    12121 tcttaagata tcttgatcct aataatgata tttgttttgc tgataaagaa aagattaatt
    12181 actggatgcc agttgactgg tacaatggtg gaatggagca tgtaaccttg catcttcttt
    12241 attcaagatt ctggcacaaa ttcctttatg atataggtta tgtaaatact cctgaaccat
    12301 acagcaaaag aacagctcat gggatgattc ttggcgaaaa caatgaaaaa atgagtaagt
    12361 caagaggaaa cgtcgtgaat cctgataaag taatagataa ttacggagca gatacattgc
    12421 gtatgtatga aatgtttata ggggattttg aaaaaagtgc tccatggtca gaaaacggtg
    12481 taaaaggatg tagaagattt atcgacaggg catacaaaat gatggacttt atgaatgatt
    12541 ctgatgacta ttcaaaagac cttttaacga ttatgcataa aacaataaag aaagtaacaa
    12601 atgattttga aaccttgaag ttcaatactg caattgcaca gcttatggct ttttcaaatg
    12661 ctgtttatct aaagggaagc ataactaaaa aggaaatgca gtcatttttg atacttctaa
    12721 atcccgtttc acctcatata actgaagaga tgtggacact atgcggattt gacggatata
    12781 ttcatgaaaa gtcctggcca gaatatgatg aagataagac tattgatgat gaaattgaaa
    12841 ttgttgtgca ggtaaacgga aaaataaaag caaaaataat gatttcatca gatatgtcca
    12901 agcaggaaat ggaatcgtta gtagaaactg aagatgcgat aattgaggta ttagaaggta
    12961 aaaagccaaa gaaaattatt gcagtaccta agagacttat aaacattgta atatagtgaa
    13021 caagatggat ttatatgata tagctgttat tggagccgga ccttgcggaa gtgttttatc
    13081 tatgcttttg gcaaaacagg gctacaaaat agctgtaatt gataagagaa accttgattg
    13141 tctccctccg acaagaagta aagcttgcgg agggctttta tcacctgatg cacaaagagt
    13201 attagcatct ttagagctga atctgcctaa taatgtgctg gttgacccac agcttttctc
    13261 agttgttaca attgatcttg aaactggtct gcaaagacat tatcagagat tttatctaaa
    13321 tatggatagg gagaagtttg accggtatct tgtttccctt attcctgata atgttgtaag
    13381 acttttttca acaatagtta aagatatttc atatgatagc agcggttatc agatatttat
    13441 ggacgacaac aaatcactaa aaaccaaaat aatcatagga tgtgatggtg caggatcaat
    13501 agtaagaaaa aagatgttta atgatttaaa aatgaatgtt aaaaactatt atgcgattca
    13561 acatgtatat gaatactcta aatacaattc aaactattat gcgatttttg ataagaatct
    13621 tactgattat tccggttggg catttcctaa gaatggaaca tttagtattg gaatggcatt
    13681 taaacaggaa tcagaaggaa atagtttttt tgatatacta atagaaaaac ttgaaaagaa
    13741 gggaatgaaa cttggtaaac ttattaaaaa agagggagcc ttattgctaa ggccgtgcgg
    13801 aattccaaaa gtgtatgcaa aaaaagataa tactgctttt ttagcaggtg aagcagcagg
    13861 atatatttca ccaagttccg cagaagggtt cagttttgct tttaatagcg catgtgcatt
    13921 agctgaagct ttcaaaaaag gtgggaacat aagtaagaat tattgtaaat taacattaaa
    13981 aatgagaatt aagctgattt taaagataat aaaagcaaga ttaatgtata atcagtatgt
    14041 aagaaaaatt attatggcac taaagattaa tgctataaga taagaagagg attttatggg
    14101 tttagaaaaa ataaagtgtt ttgtactaga tatggatgga actatttatc ttggaaatag
    14161 tctttttcct tacaccaagc attttttgga agttgcagaa aaatgcggta ttgatatttg
    14221 gtactatact aataattcat ctaagaatgc aaaattttat gtagaaaaat taaaaagaat
    14281 gagcattgat tgtgaaagtc ataaaatgct catatctaac caggttatta tccaatatat
    14341 attaaaaaat catagaaact gtaaagttta tgttgttggt acagaatatc tagtaaatga
    14401 ttttgtagat gcaggaatca atgtaaccga caaaaactgt gatatggtag ttatgggatt
    14461 tgatactact ttgacttatg aaaagcttgt aaaagcatgt gattttgtca gaaacggagc
    14521 cactttatat ggtgtgaata tggattataa ctgtcctacc gaaacaggat tcattcccga
    14581 ttgcggttca atatgcaaac ttgtgcttgc ttcaacagga gtacaagcag aattctttgg
    14641 aaaaccatca catcatacnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnntc
    14761 taatataatt attaaagcaa aaaagaagat cacagttaca gctattacca tggcagcaat
    14821 aaccaaaaaa gcaaacccca acatttccgc atgtgctata gtctcaaaaa gctgtttcat
    14881 taattcctcc tcaaaatata attattcatt ctggtctgaa atttctttat cctcattttt
    14941 gttttcatgt tttcttgttt tatagaaggt tattactatt aaaactaaaa gtaaaacact
    15001 tattccaaga gatatcgcaa attctggtac aaaaggtttg tcatattcaa cttttgtagt
    15061 ttctacatca atatcttcac tatattgggt aatatctata tcgtttttaa ctggttttaa
    15121 tgaacttgct gcaacaatgt atgactttcc aatatagtta ccgttatacg agtatgttac
    15181 ctctcccatg atatccccga tatcaacttg tgaatatata atatcacttc tataatcaaa
    15241 tgtcttgtca agcgtccatg attggctgtc atatggaaca agatgttcaa gtctcattgc
    15301 atttgctata ttaattcctt cgtgattgtc aattatctgt cctgcttgtg cgatgcactg
    15361 tataccaaaa tcttcataag aatgagccat aagttccaag gtaatttctg cggcttcaac
    15421 cctgtctttg acaccagtta taacagtaat tacctcacgt ccggaataat ctgaagatag
    15481 tgctactaat acattttgag cttttgatgt gaatcccgtt tttccagcag taacagtgaa
    15541 gtcatatcct gtatttgtac caagaatcat attggtgttt ttgataagat caaaattctg
    15601 tctcaaattt gtggcaggta aggaatattc tttttttgcg caaattgtcg atatagtaaa
    15661 attagaaaaa gcagctcttg cgattatggc taaatccaaa gctgtagtgt aatgttcatc
    15721 atcatgaagt ccatgaggtg taataaaatg agaattaact gcacctaact cttgggcttt
    15781 ttgattcatc atgttcatga attcttcttc tgaaccggcc acattctctg cgattactaa
    15841 agctacatca ttaaaactcc ttattaacag accatgaaga agagaataaa agtccatagt
    15901 ctcatcagga agaagttcta attcaccgct tccatcctct aaaaacctgg ttgcattttt
    15961 tgatgtgaca aatattttat ttaaaggaca attttcaact gcaacaaggg ctgtcaaaag
    16021 cttagtagtg cttgcgggat agattttcat atcactatta tatgaataaa gaacatctcc
    16081 tgtctcaaaa tccatagaca ggtaagaaag acatgaatca ggattgatca ctgtctgtgc
    16141 ttgtccttgt atactaatgc tgaacaagct tatagttatt aatattacaa gtatggatga
    16201 aatacctttt ctcatatctg cctttctttt gtagattata ctacatttga gattaatatt
    16261 gaatcggaaa atattactgc acctagataa aatgtatttt taaatcattt ccattttgtc
    16321 tattgtctga taacttctga tatgttagtt atgagatatg aaaaagcaca ttccgacatt
    16381 tttaatattt ggaattcttt tatgtataat gattattatg ataataagag cagtaaataa
    16441 aaagattgta tttggaaatg atgatacatc caatacatat gagcagatgg aagaaaaacc
    16501 tgaaacttta ttaatatgta taaaaggaga aattaaaaat ccaggaattt atgaaataga
    16561 aaaggggagt atactaaacg atttggttat attagcaggc ggttttactg aaaatgcagg
    16621 gaaagacatt aatctggttt atgagcttaa cgagaatctt accatatata taaggtcaga
    16681 agaagaatct ggaggaatgg atattataga tgataaagat attatttttc aatctgatga
    16741 tgaaaaagtg ttatttgaaa ataaagtaga tattaataca tcagatattc agactctttg
    16801 tcttttacca ggtataggag aaaaaacagc tgaggaaatt attagatata gagattctat
    16861 aggtatattt gaaactatag aagatattat gaatgtaact ggaatcaaaa atgctaagtt
    16921 cgaaaaaata aaggattaca taacaatagg gaattagcat tgacaaagat gctttgataa
    16981 aatatacttt catatgctgc ggatgtggcg aaattggcag acgcgttggt cttaggagcc
    17041 aatacgcaag tgtgcaggtt caagtcctgt catccgcacc atgtacctgt tatcaattta
    17101 ttctgatagc aggtattttt ttgtgtataa tcataatgga atataaaaaa ttggaaatag
    17161 gtgaaaaatg aaaagaaaac gtcttgatag agatatttgg acttcaatag tagaaaaaga
    17221 gtattttcag gaaaatataa atgaaccaga ttttagcggt attctttcag ttctttatct
    17281 taagaaagtt tttcctgatt ctgtttggaa tgctaatcat gaatcatttg cagttgcatc
    17341 ttcaggcatg aaatggttgc aattactttc aagtgagaag aattatctat taactgcaat
    17401 gataagcgat aaaatgaaat agtctgttac tatattgatg tgatagcagg atatggattt
    17461 gatca
//
LOCUS       DAOSXM010000042        17142 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2111
            NODE_2101_length_17142_cov_9.489905, whole genome shotgun sequence.
ACCESSION   DAOSXM010000042 DAOSXM010000000
VERSION     DAOSXM010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864178
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 17142)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17142)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:24:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,261
            CDSs (total)                      :: 2,229
            Genes (coding)                    :: 2,218
            CDSs (with protein)               :: 2,218
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 3 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17142
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2101_length_17142_cov_9.489905"
                     /isolate="AMR_MDS_2111"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..907
                     /locus_tag="PLQ68_06620"
     CDS             <1..907
                     /locus_tag="PLQ68_06620"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HXK72361.1"
                     /translation="RMKKYQLKNNILIGIVIGLCIIVIAMASVIWMNYDYIFFKFFIS
                     KDYIFTDTLDKMYKDTLSVDIKSEKEYYKYFDNIAISLISEKIKTTGNDPYTYQYTPS
                     QYSSYIENRSEKANMTHTELLNENTILLKYTNFSDESMDIFKKSVKTINDYSRIIIDL
                     RDNTGGGLDEAYELSSYFLNPDDISYIIKYRDKLEPAIVKSNKILDFQKIIILQNNNT
                     ASASEIFINALKENLNNVITVGVKSFGKGIGQTTYSIKNGFHVKATTFLVLTPNQNSI
                     HSIGIVPDYLYEDEETIIDFCMSLE"
     gene            complement(910..2010)
                     /locus_tag="PLQ68_06625"
     CDS             complement(910..2010)
                     /locus_tag="PLQ68_06625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72362.1"
                     /translation="MRNIKITKIIRIFIILFATLMLSSCVNKVKPYDIPVFNIRNEIY
                     ETVELKKQSVHDFVQFKLVLDTLSGTRFSFDYSQQEQCEIIIDESGIYVKENDVLAIK
                     HSKELQNELRLKNENLELKQLYYNQLYEKYINSSQGYIDVLLAELDVQEAEYSVEKTL
                     DKIEDLKVRAPKDGVIEKVVETYTNVTDEFGNVSKVFTVTYTYEIDNYVMRLNINNQD
                     AVTSILNRIWEGEYPIIQIYDVNGVYYDTKVKDVSVSGFYNYRTASYDRALTVLLEFV
                     KQEGVDYSSVVRYALYYDQILKELDDAIVINTDCVYGGTDDLKYVYILVDGKKMTRYV
                     TLGNEVYNSTSVIVLDGLSEGDLLITDITLEQ"
     gene            complement(2007..3116)
                     /locus_tag="PLQ68_06630"
     CDS             complement(2007..3116)
                     /locus_tag="PLQ68_06630"
                     /inference="COORDINATES: protein motif:HMM:NF024923.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HXK72363.1"
                     /translation="MKKMFKAKKILVLLMFLLSVSVLSGCTYFYPAEQKIYIPELIEP
                     EKPDLLIIEIQKGDIVKGYLLGEDDQTFCEFVNDPTKTTFVNADIEEAYVKQIYVSEG
                     DFVKEGDILIEYEYEFDKDTEFLLNIDKRRAELKYEKAVTQLEQGKLSSEALELYRNA
                     YSTATSKLDEFYALEEKYTLRAPCDGTIVEIEGYSVSSNDKHNVTFQISNIEDGIIII
                     YADSNNDVAAFPLYIFSKGSKARISNEELGIEANAYVKYNNTDFKKNYEGYLDTIHGS
                     NMVYMELEFEESSFPESINFNQTFTVSLIQKEIYDVVLVPRSAVEETAWDQYYVYKLN
                     ENNDLEKVIIEIGENNENYYEVKEGLEEGDKVLIR"
     gene            complement(3100..3999)
                     /locus_tag="PLQ68_06635"
     CDS             complement(3100..3999)
                     /locus_tag="PLQ68_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015254322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXK72364.1"
                     /translation="MIFCDGLVKIYKSKDTEVVALQGLELEIKKGEMMAIIGNSGSGK
                     STLMNLLGGLDRPSAGILIVDGKNLLRMRKNEILEYKKKKVGFVWQNNARNLIPYLPA
                     LHNVELPMLLAGKKNARKKATELLEIMGLKDRMHNRLSQLSGGEQQRVAIAIAMANDP
                     VILLADEPTGAVDTATAEIIYNIFRKLNKEFAVTIIIVTHDRAVAKNVDRVVTIVDGR
                     TGSEYIRKKSYKEQIQELDRMSGVEESETHEEMIVVDRQGRLKIPEEYLELANIPNNS
                     KVRLKMQDDKILIYGKDENEEDV"
     gene            complement(4128..4799)
                     /locus_tag="PLQ68_06640"
     CDS             complement(4128..4799)
                     /locus_tag="PLQ68_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013499757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXK72365.1"
                     /translation="MSELIKATKICRDFKQGSNIIHALSDVNISINSKTLTMLAGRSG
                     SGKTTLMNIAGALDRPTSGKIYFDSIDLTDLSESKKDDIRRKNIGFVFQSVALISVLN
                     AYENVEFGLRMAGVNPKYWDDLVRESLKFVGLEKRMKHKPHQLSGGEQQRVAIARAIV
                     SRPKLIFADEPTAELDTKSGLKIVNLFRHLVDEENMTIVMTTHDPSMIELSDDVIKLE
                     DGRVI"
     gene            complement(4801..7632)
                     /locus_tag="PLQ68_06645"
     CDS             complement(4801..7632)
                     /locus_tag="PLQ68_06645"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HXK72366.1"
                     /translation="MLRLIIRKMFNNKWMVACLIIGCAFAIAVASAIPMYSAGIYDNL
                     LVNEFKNQQQRDNTFPFRMWYNATFFGLDNSSSKVNFDQLDKIFYDDYIKNMPLDTVF
                     EYKNTTTKVYSVFDQQNYLLKQQAIEKGETLSYRNLNVKADYSITAAANGENMVVIEA
                     GRIFEKSTEDDVIEAVITQRSAALFRLSVGDYYYLYNRPVDEQVAAKIKIVGIAKVSE
                     EASSFFTLSDKTFLISFEKMEELISNSKITLQQANWFAAFDYTKISILDTDSILSASA
                     VLKQDFQNSGLLLSSSNNYVELIKTYESNTLVMQRIFLLLIIPVILMIAMYVVMISQL
                     MMEREKSEIATLESRGASRGQIFKIYFGQGIILSLISLVLGILLARGICRLLGASNGF
                     MEFVNRTAIDAKFSMTSFIYALICCLIYMIMILIPAYKASKYSIVQHKQNIANGSKKS
                     FWQVIFLDLILLAISIYFLYSYNEISDYLKNSEQSFIDFTLFIAVTLFVMGLGLLFIR
                     LFPYLIKFIFFIGKRLWRPASYTAFTHVARGGNYKQFIMIFLILAISIGIFNANTGRT
                     INDNIEDNIRYRIGADALVITGKEHFDPVTGTTRIIEDSGRELEIMADKICESEYVDN
                     YCVIYQSNMELTQSQSQIPTEFIGINPYTFGKTTSLRNDLNGGFHLNYYLNLLSTYPQ
                     GVIISKKLADEYEVTQGDYFNYEYSAFSEQIFMENILVVGIVDYWPGIIGDSFIVLTE
                     DSYITMKYISMRGKILIDKKDGYLDSILLEDLKTYLGHGEYIYSSAFTDYELTLEKKN
                     SILNGMNGILTLDFLISMIICCVGFIIFWIISVRQRVLQFGIFRAMGMKKSELYRMIF
                     FEQLMVSFVSVIMGIVIGGLTTQLFLPLIERLFKLGELVVPFTAYQDGIDYIRIYVIT
                     GLSLIVGLSVLLRFISKLKIDQALKLGED"
     gene            complement(7637..8629)
                     /locus_tag="PLQ68_06650"
     CDS             complement(7637..8629)
                     /locus_tag="PLQ68_06650"
                     /inference="COORDINATES: protein motif:HMM:NF024923.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HXK72367.1"
                     /translation="MKKKIAFFLTVYILLILLTGCMSLFPQEDEMLSIPVPTFVQVEY
                     RTIKPQRGDIIKRVNVLGTVVADYHESIDMKFNVDGYINEIFVENEQYVEEGEIIATL
                     KEPDNKEKIEQEYTESLNSYLNIQENYSQNRATEYDLRKAEIAYEHAKEKYDELLYYL
                     ESLQLKALKSGYIVSLKEMQESDPIGKTLTVCAIAEKIDKMVQATTTTDNVSKLPTGT
                     EVTLVIKDTEYKGYVSNSSGNTLRFKLFEDMTFMQNGDIIEVYLVLETAYDVLMVPST
                     VITFTEDNKGDIRVLSDGAPVTYSITFGLRSDTHVEIFPDQGITEDTLIITGVR"
     gene            complement(8722..9426)
                     /locus_tag="PLQ68_06655"
     CDS             complement(8722..9426)
                     /locus_tag="PLQ68_06655"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXK72368.1"
                     /translation="MKLTVLGNSGPYPSAERPCSGYVVELNNKLIILDLGSGTLMNFQ
                     KSYNIEDISFVILSHLHFDHMSDILVLRYALQVKKIKIDLYLPKQPKNISSVLECDEF
                     NLIFINQNTTIKRDGFDISFTKTTHPVETYAIKVNNKFVYSADLSEPDDLVNFVHSVD
                     TLLIDSAFLTIQKGNKKLFHLSASECAMIASKHKIKNLLLTHLPPDAKQAMYIDEAKL
                     FFDNVRVVQVMQEIDM"
     gene            complement(9401..10498)
                     /gene="murG"
                     /locus_tag="PLQ68_06660"
     CDS             complement(9401..10498)
                     /gene="murG"
                     /locus_tag="PLQ68_06660"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HXK72369.1"
                     /translation="MKILIAGGGSAGHINPAISIAKEIVKHKPDSKILFIGSQKGLEK
                     DLVPREGFDIKFIEIEGFKRPLTFKSIKTVFIMIKGFFQANKIVREFKPDISIGTGGF
                     VSGPAIFAAYLHKSKIMIHEQNAYPGVTNRMLSRYSDVAMTSYESASKFFIKSKKTVL
                     TGNLIDDCFSSMEKYKPTEGKKTIVIIGGSQGAMTINNAVIGMINDHMNKDDFNLIFA
                     PGKKYFQQVVSKVINKLNNVMIHDYIYERNTIYAKADLMVIRGGAISISEILAMGKPA
                     IVIPSPYVPDHAQEKNAQMIYNNGACEIMYDNETLTSHALYEKIYSLLNNNQRLIEIS
                     KKAYSLGIRDANERVYKEFLEVSDEINSVGK"
     gene            complement(10495..11613)
                     /gene="ftsW"
                     /locus_tag="PLQ68_06665"
     CDS             complement(10495..11613)
                     /gene="ftsW"
                     /locus_tag="PLQ68_06665"
                     /inference="COORDINATES: protein motif:HMM:TIGR02614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HXK72370.1"
                     /translation="MLRSNGRNKTDFVIFLFALGLTAFGLLMIFSAGAPYAKSRFNDI
                     YYFIKDQMFAAVIGLVLMLIASSVSYKLYRKLSWIIFPIGLILLILTYIPGIGVAYSD
                     ARRWINIGFEFQPSEIYKLAFIVFLSKIILDFKKLDKPLIGYFVTMGITLSSALIIFF
                     QPHLSAAIIITLVGFVIIFIGGTKIKYLVLTVLPVITLAVIGAFTYDYMKERIMTWLD
                     PFAYPTDLGYQIIQSLYAIGSGGLFGVGLGKSTQKFLYIPEPQNDFIFAILAEELGYI
                     GVFLVIAMFCMLLWRGIRIAIYCEDMFGTLLATGITSLIFIQAALNIAVVTGLVPNTG
                     ITLPFFSFGGTSLVMFLTQIGILLNISKQSVDKRELNV"
     gene            complement(11657..12610)
                     /gene="mraY"
                     /locus_tag="PLQ68_06670"
     CDS             complement(11657..12610)
                     /gene="mraY"
                     /locus_tag="PLQ68_06670"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HXK72371.1"
                     /translation="MIIAAIVTFVLTVILGILMLPVLKKMKIGQVVRDDGPKEHLKKT
                     GTPTMGGIIFIIPILACTLFVGWKDPLVLASCALILVSSIVGFIDDFLKIRKKNKDGL
                     KPIFKTIFFIVISLLYAIFIVYFTDIGSDIIIPFTNTSKIFYVPGWIYVIFIVLFYNA
                     VINAVNFTDGVDGLCASVTSAVFIFFAVSSQIIFKNENMTYLSIIVIFAMIGYLVFNV
                     HPAKLLMGDTGSLALGGVVTAISINLRIPWIILIVGIIYVIEILSSVIQVMHYKRTKK
                     RIFLMAPIHHHFELMHWSENKIVIIFTAVTLAGGILAFLMI"
     gene            complement(12607..13959)
                     /gene="murF"
                     /locus_tag="PLQ68_06675"
     CDS             complement(12607..13959)
                     /gene="murF"
                     /locus_tag="PLQ68_06675"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HXK72372.1"
                     /translation="MNIKLSSIYNIFQNLYCKDIDKNITGVSIDTRTLKENDLFFAIK
                     GDNFDGHCFVNKALSLNAAAAVCSKEDNYNGDVIYVNDTTEALQDFARYYRNMFDIPF
                     ISITGSNGKTTTKELINTAISTEMKTCYTIGNLNNQTGVPLTLFSLNENTQAAVIEMG
                     MNSIGEIRKLSYIVKPKIAVITNIGTSHIGMLGSKENIFKAKTEVLDYVDNGYLVVNG
                     DDDFLSSIKDSDNYKVIKVSIYNNDADYIAQNIVQNEDNTYSFTVNSKNINLSIPGFH
                     NIYNALCAFAVSDLLKVNTDKASKAISECKNQKMRTQISVINGYTVINDAYNANYDSM
                     KASLELLGNYNKRKVAILGDMLELGDYEKYFHIKAGEIVTDNNIDVLIAIGKNADNYY
                     QGAVDSGMRSACIYRYASIEESKNTIKDIIQKDDVILLKGSRGSAMEKLMDIFEEEII
                     "
     gene            complement(13956..15449)
                     /locus_tag="PLQ68_06680"
     CDS             complement(13956..15449)
                     /locus_tag="PLQ68_06680"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HXK72373.1"
                     /translation="MDIHQLLSKTDYIKASHLKDVIISGISFDSRKTKKNDIFVAIDG
                     ADEDGHKYIDNAIQKGVAAIVITKEYDFYRSKYPQVATILVEDSRIFLAQSSALFFDY
                     PSKNMNIIGITGTKGKSTISYMIKNIFDKADLKSAVIGTIGISYNNKLIVTNTTTPSS
                     YELNQIFSDIRNEKISNCIMEVSSISMKMNRVYGLHFNIGMYTNLTQAHITQREHPTF
                     EDYYNSKKKIFEKSKVMLVNIDDENVEKALNEYLVSDHYIKTDNRIISIGIDKKADII
                     AENIVYKNDKSSFTYKGLNKCFDVLIDIPGRFNIYNSLFAITAALLNDVDIIHIQNGL
                     SDVQVRGRCEKVDIGKDYTMMIDYAHTPDSLLKILQAVRETKTSGRIITLFGCGGDRD
                     KSMRPMMGKISGENSDFTIITSDNSRTEKPEKIISMIEDGIKQTNGQYICITDRTEAI
                     RYAMKIAKKDDIVILAGKGNETYLDVMGVKTHYDEREVIFGILEGKI"
     gene            complement(15464..>17142)
                     /locus_tag="PLQ68_06685"
     CDS             complement(15464..>17142)
                     /locus_tag="PLQ68_06685"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HXK72374.1"
                     /translation="LASHAIGFAGDDNQGLFGLELSLDKYLKGTDGKILTELDVAGTE
                     LPFAFESRIEAIDGFDVITTLNTDIQRIAEKIVEQAIEDNKSEDGAVCIVSNSKTSEI
                     LAMVSSPGFDLNNPRLLPEGYTIEGWTGLRSSDVQLLYSTVWRNKALMDTYEPGSTFK
                     SITTAMALEEDIVELDTIVDDFPVTVFGSTIHCWSKEYPHGTETFLQAVYNSCNPVFV
                     KISHLIGIKTFYEYVEAFGFKDNTGIELIGEGTSIFHDEPTKLDMSVASFGQRFQITP
                     IQMVSAYNAIANGGYLIEPSLTDAVIDKQGNTVYDFEPKVVRQVISQDTSDQLRMVLE
                     GVVKEGGGKNAYVEGYRVAGKTGTSETIVDDVYISSFIGFAPADNPVITVLFIVFNPQ
                     GDSYYGSQVAAPYAKLVFEESLGVLGVEKDGVVNNVNSVYVPNLNGFNVEKATQKLKS
                     YGLSYQIEGSTAGEDVTIMYQYPESGTKINQDGVIILYTYVPENKKTVRIPYLIGKQE
                     QEVIKTLKDLGLNVNVDGNGTCISVQYPAGAYVEKGTIVNITFRYTDYLD"
BASE COUNT         5557 a         3149 c         2231 g         6205 t
ORIGIN      
        1 aagaatgaaa aagtatcagt taaagaataa tatattaata ggaatagtaa taggattgtg
       61 tattattgtc attgctatgg ctagtgtaat atggatgaac tatgactaca tattcttcaa
      121 gttttttatt agcaaggact acatatttac tgatacttta gacaaaatgt ataaagatac
      181 attatcagta gatataaaat ctgaaaaaga atactacaag tattttgata atattgctat
      241 cagtcttatt tctgaaaaaa taaaaactac aggaaatgat ccttatacct atcagtatac
      301 tccatctcag tatagttcat atattgaaaa tcgttctgaa aaagctaata tgacacatac
      361 tgaattatta aatgaaaata cgatacttct taaatatacg aatttctccg atgaaagcat
      421 ggatattttc aaaaaatctg taaaaacaat taatgactac agcagaatta ttattgactt
      481 aagagacaat acaggaggtg gtcttgatga ggcatatgaa ttatcctcat attttcttaa
      541 tcctgatgat atttcatata taattaaata cagagacaaa ctggaacctg ctattgtcaa
      601 atctaataaa atattagatt ttcagaaaat tattatcctt caaaataata atactgcctc
      661 tgcttcagaa atatttatta atgcacttaa ggaaaatctt aataatgtaa ttactgtagg
      721 tgttaaatct ttcggtaaag gtattggtca gactacatat agcataaaaa atggatttca
      781 tgttaaagca acaacatttt tagtactcac acctaatcag aactcgatac attctatagg
      841 aatagttccc gactatttat acgaagacga agaaacgatt attgactttt gcatgtcttt
      901 ggagtaactc tactgctcaa gtgtaatatc tgttatcagc aagtctcctt cagataatcc
      961 atcgagtaca attactgacg ttgaattata tacttcattt cccaaagtta catatctagt
     1021 catctttttt ccatcaacaa gaatatacac atattttaaa tcatctgtcc cgccatacac
     1081 acaatctgta ttaattacaa ttgcatcatc aagttctttt agtatctggt cataataaag
     1141 tgcatacctt actactgatg aataatcaac tccttcttgt tttacaaact ctagcagcac
     1201 agttagtgct ctgtcgtaac ttgctgttct gtaattataa aaaccagaaa cagaaacatc
     1261 ctttactttt gtatcataat atactccatt aacatcatat atctgaataa ttggatattc
     1321 gccttcccat attctgttga gaatacttgt tactgcatct tgattgttaa tattcaatct
     1381 cataacataa ttatcaatct catatgtgta agtaacagta aatactttgc tgacattccc
     1441 aaattcatcg gtcacatttg tgtatgtttc aacaaccttt tcaattacac catcctttgg
     1501 tgctctaacc ttaaggtctt cgattttatc taaagttttt tcaacagaat attctgcttc
     1561 ttgaacatcc agttctgcaa gcagaacatc aatatatcct tgactagagt ttatatattt
     1621 ttcgtataat tgattataat ataactgctt aagttcaaga ttctcatttt taagccttaa
     1681 ctcattctgt aattctttag agtgttttat agctaataca tcattttctt ttacataaat
     1741 gcctgattca tcaataataa tctcacattg ctcctgttgt gaataatcga aagaaaatct
     1801 agttccagaa agagtgtcga gcacaagttt aaattgtaca aaatcatgaa cactctgttt
     1861 ttttaattca actgtttcat atatctcatt acgaatatta aatacaggta tatcatatgg
     1921 tttaacctta tttacacagg aagacaacat taatgtagca aataatatga tgaaaattcg
     1981 tattattttt gtgattttaa tatttctcat cgtattaaca ccttatctcc ttcttccagt
     2041 ccctctttaa cctcgtagta attttcattg ttttcaccaa tttcaattat tactttttct
     2101 aagtcattat tctcgtttag cttatataca taatactgat cccatgcagt ttcttcaaca
     2161 gcacttcttg gtactaatac aacatcataa atttcttttt gtataagtga tactgtaaat
     2221 gtttgattga aattaattga ttctggaaaa ctgctttctt caaactccag ctccatgtaa
     2281 accatattac ttccgtgtat tgtatctaaa tacccttcat agtttttctt aaaatcggtg
     2341 ttattgtatt taacatatgc atttgcttcg attccgagtt cttcatttga aattcttgct
     2401 tttgaacctt tagaaaaaat gtacaaagga aaggcggcaa catcattgtt actgtcagca
     2461 taaataataa tgattccatc ttctatgtta ctaatttgga aagtaacatt atgtttgtca
     2521 tttgaagaaa cagaatatcc ttcaatctca acaattgtac cgtcacaagg tgctcttaat
     2581 gtatattttt cctcaagtgc gtaaaactca tctaatttac tagttgctgt tgagtatgca
     2641 tttctataaa gttccaaggc ttctgatgag agtttacctt gttctaattg tgttactgct
     2701 ttttcatatt ttaattctgc tcttctttta tcaatattta ataggaattc agtatcctta
     2761 tcaaattcat actcgtactc aataagaata tctccttctt taacaaaatc accttcagat
     2821 acatatatct gctttacata agcctcttca atgtcagcat ttacaaatgt agtctttgtt
     2881 ggatcattaa caaattcaca aaaagtctga tcatcctcac caagtaagta acccttaaca
     2941 atgtcacctt tttgaatttc gattataagt aaatcaggtt tttcaggttc aatgagctca
     3001 ggtatgtata ttttttgctc agcaggataa aaatatgtac atccagatag tacagatact
     3061 gataacagaa acattaataa tactagtatt tttttcgctt taaacatctt cttcattttc
     3121 atccttgcca tatatcagta tcttgtcatc ttgcattttt aacctgactt tgctgttatt
     3181 tgggatatta gcaagttcaa gatactcttc tggaattttc aatcttccct gcctatcaac
     3241 tacaatcatt tcctcatgtg tctctgattc ttcaacacct gacattctgt ctaattcctg
     3301 tatctgctct ttatatgatt ttttacggat atattcactt ccagtacgtc catcaactat
     3361 agtaactacc ctgtcaacat ttttagctac cgctctatca tgagttacaa ttattatagt
     3421 aactgcaaac tctttattga gttttctgaa aatattataa ataatttcag ctgtagcagt
     3481 atcaactgct ccagtgggtt cgtcagcaag taagataacc ggatcattcg ccatagcaat
     3541 tgcaattgct actctttgct gctcacctcc tgataattgt gataatcggt tatgcatccg
     3601 gtccttaagc cccattattt caagaagttc tgttgctttt tttcttgcat tttttttacc
     3661 tgctagaagc attggtaatt caacattatg gagtgcagga agataaggta taaggtttct
     3721 tgcgttattc tgccaaacaa agcctacctt ctttttttta tattctaaaa tttcattttt
     3781 cctcattcgt agaaggtttt taccatcaac aattagtatt cctgcagatg gtctgtctaa
     3841 ccctcctaat aaattcatta atgtagactt tccacttcca ctatttccta taatagccat
     3901 catttcgcct tttttgattt ccagttcaag accctgtaat gctactactt cagtatcttt
     3961 ggatttatat attttaacta atccatcaca aaatattata tgatcaggtg tatagccagg
     4021 tatttctgaa tcatccttac cataaaaatt ctttgcagta atgtccctag ttattgactt
     4081 ttttggacga attatcgtta ataactttct ttttaaatca gctattttca tataaccctc
     4141 ccatcttcca gttttattac atcatcagaa agctcaatca tgctcggatc atgcgttgtc
     4201 ataactattg tcatattttc ttcatcaaca agatgtctga aaagatttac aattttcaaa
     4261 cctgacttgg tatccagttc agctgtaggt tcatctgcaa aaataagttt tggtctggag
     4321 acaatagctc tggctatagc aaccctttgc tgttctccgc ctgataactg atgaggctta
     4381 tgcttcattc ttttttccaa tccaacgaat ttaagtgatt ctcttactaa gtcgtcccaa
     4441 tattttggat taacacctgc cattcttaaa ccgaattcta cattctcata tgcatttaaa
     4501 acagaaatta atgcgactga ctgaaaaaca aacccaatat tcttccttct tatatcatct
     4561 ttcttacttt cacttaaatc tgttaaatcg atactgtcga aatatatttt tcctgaagta
     4621 ggcctatcga gtgcacctgc tatattcatc aaggtagtct tacctgaacc ggaacgacca
     4681 gcaagcatcg ttagcgtttt agaatttatt gaaatgttta catcagataa tgcatgtatt
     4741 atattgcttc cctgtttgaa gtcgcggcag attttagttg cttttatcaa ttcagacata
     4801 ttaatcctcc cctaacttca atgcttgatc tatcttcaat ttggatataa accttagaag
     4861 tactgataat cctactatta aagataaccc tgttattaca taaattctta tataatctat
     4921 tccatcttga tatgctgtaa atggaactac aagttcacct aatttaaata gtctttctat
     4981 cagtggtaag aataactgag tagtcaaccc tcctataaca atacccatta taactgatac
     5041 aaaagatacc ataagctgct caaagaaaat cattctatat aactcacttt tcttcattcc
     5101 catagcacgg aatataccaa attgaaggac tctttgtcta acagatataa tccagaaaat
     5161 tataaatcct acgcagcaga ttatcatgga tataagaaaa tctaatgtta atattccatt
     5221 catgccgttt agaattgaat ttttcttttc aagtgtcagc tcgtaatcag taaatgcgct
     5281 actataaata tattcaccat gacctaaata ggtttttaaa tcttccaaca gtatactatc
     5341 gagatatccg tcctttttgt caataagaat ttttcctctc atcgaaatat atttcattgt
     5401 aatataacta tcttctgtta aaacaatgaa actatcccct attatacctg gccaataatc
     5461 aactattccg actaccaaaa tattttccat aaatatctgt tctgaaaagg cagagtactc
     5521 ataattaaaa taatctcctt gagttacttc atattcatcg gctaactttt tcgaaataat
     5581 tacaccttgt ggataagtag ataataaatt tagataatag tttagatgga atccaccatt
     5641 caaatcattg cgtaaacttg tagttttacc gaatgtatat gggtttattc ctataaattc
     5701 tgttggtatc tgtgattgag attgtgttaa ttccatattg ctttgatata taacacaata
     5761 attatcaaca tattcagatt cgcatatttt atcagccata atttccagtt ctctacccga
     5821 atcttcaatt atccttgttg tgcctgtaac tggatcgaaa tgctcttttc cagttattac
     5881 taatgcatcc gcgcctattc tgtatcttat attgtcttca atgttatcgt ttatggttct
     5941 accggtgttc gcattaaata taccgatact aattgctagt atcaagaaaa tcataataaa
     6001 ctgcttatag ttacctcctc tggcaacatg agtaaaggca gtatatgatg caggtctcca
     6061 taatcttttt ccaataaaaa atataaattt aatgagataa ggaaatagtc ttataaacaa
     6121 taaacctaat cccattacaa ataatgtgac agctatgaat aatgtaaaat caatgaatga
     6181 ctgttctgaa tttttaagat aatcactaat ctcgttatat gaatacaaga agtaaatgga
     6241 gatcgctaac aagatcaaat ctagaaaaat aacttgccag aatgattttt ttgaaccatt
     6301 ggcaatattt tgcttatgct gtactattga atattttgag gctttatacg caggtattaa
     6361 aatcataatc atgtaaataa gacagcaaat aagcgcataa ataaaggaag tcatactgaa
     6421 ttttgcatca attgcggttc tgtttacaaa ttccataaac ccatttgaag ctcctaataa
     6481 acgacatatt ccccttgcaa gtagaattcc cagtacaaga gaaattaagg acagaataat
     6541 accttgtcca aaatatattt taaatatctg tcctcttgat gctcctctgc tttcaagtgt
     6601 agctatttct gatttttctc tctccatcat aagctgagaa atcattacaa catacatagc
     6661 tatcattaat ataacaggta ttattaaaag aagaaaaatc ctctgcatta ccaaggtgtt
     6721 actctcgtat gtctttatta attccacata attatttgat gaagaaagta ataatcctga
     6781 attttgaaaa tcctgcttaa gtacagcaga agcgcttagt attgaatcgg tatctagaat
     6841 acttatttta gtatagtcaa atgctgcaaa ccaatttgcc tgttggagcg taattttcga
     6901 atttgatata agctcctcca tcttttcaaa acttattaag aatgttttat cagaaagagt
     6961 aaagaatgaa gatgcttctt cgctaacttt agcaatacct acaattttta tcttcgcggc
     7021 aacttgttca tcaactggcc ggttataaag ataatagtaa tctcctactg ataatctgaa
     7081 caaggcagca gatcgctgcg ttatgacagc ttctatcaca tcatcttccg tactcttctc
     7141 aaatatcctt cctgcttcaa tcacaaccat attttcacca ttagcggctg ctgttattga
     7201 ataatctgct ttaacattga gatttctata agataaagtc tctccttttt ctattgcctg
     7261 ctgtttaaga aggtagttct gctgatcaaa tacagaatat acttttgtag tggtgttttt
     7321 atactcaaac actgtatcca aaggcatgtt tttaatataa tcatcataaa atattttatc
     7381 caattgatcg aaattcactt tagacgatga attatctagc ccaaagaaag tggcattgta
     7441 ccacattcta aagggaaatg tattatccct ttgctgttga tttttaaact catttactag
     7501 aagattatca taaattccag ctgaatacat tggtatagct gatgcgacag caatggcaaa
     7561 tgcgcaacct ataatcaaac atgctaccat ccatttgtta ttaaacattt ttctaataat
     7621 cagacgtagc atactcttat ctaacccccg ttataatcaa tgtatcttcc gtaataccct
     7681 ggtcaggaaa aatttctaca tgtgtgtccg atcttaaacc aaaagtaata ctatatgtta
     7741 ctggagcacc atcagacaaa actcttatat cgcctttatt atcttcagta aatgttataa
     7801 cagttgaagg aaccatcaga acatcatatg cagtttctag aaccaaataa acttcaataa
     7861 tatctccatt ttgcataaat gtcatatctt caaacaattt aaatcttaat gtgtttccac
     7921 ttgagtttga tacatatcct ttatattcag tatcttttat cacaagtgtt acttctgtac
     7981 ctgtaggtaa ttttgataca ttatcagtag ttgttgttgc ttgaaccatt ttatcaattt
     8041 tttctgctat tgcacaaaca gtgagtgttt ttccaatagg gtcggattct tgcatttcct
     8101 ttaatgaaac tatatatcct gattttagcg cttttaattg taatgattct aaataataaa
     8161 gcagctcatc atatttctct tttgcatgtt catatgctat ctctgctttt cttaaatcat
     8221 attcagtagc tctgttttga gaataatttt cctgtatatt cagataacta ttcaatgatt
     8281 cagtatattc ttgttcaatt ttttccttat tgtccggttc cttaagggtg gctattattt
     8341 cgccttcctc aacatactgc tcattttcga caaatatttc atttatatat ccatcaacat
     8401 taaatttcat atcaatagac tcatgataat cagcaactac tgttcctaag acattaaccc
     8461 tttttataat atcacccctt tgaggtttaa ttgttctgta ttcaacttga acaaatgttg
     8521 gtacaggtat gctgagcatc tcatcttctt ggggaaataa agacatacat cctgttaata
     8581 aaattaataa tatataaact gttaagaaga aagctatttt ctttttcatt tcatttcctt
     8641 cagacaatat aatagactat attttttgta ttataaagca ttttaattga attgtcataa
     8701 aaaccatata aaatattaga attacatatc aatttcttgc attacttgaa caactctaac
     8761 attatcaaaa aatagtttag cttcatcaat atacatcgct tgttttgcat caggaggaag
     8821 atgagtaagt agtaaattct ttatcttgtg tttgcttgca atcattgcac attctgatgc
     8881 tgataaatga aacaatttct tattaccttt ttgtattgtt agaaaagcag aatcaattaa
     8941 taaagtatct acactatgca caaagttaac taaatcatct ggctctgata aatctgcaga
     9001 gtaaacaaac ttattattga ctttgattgc ataagtctca acaggatgtg tggtttttgt
     9061 aaaagaaata tcaaatccat ctcgtttaat agttgtattt tggttgataa atattaaatt
     9121 gaactcatca cactctaata ctgaagatat atttttgggc tgttttggta aatataaatc
     9181 gatttttatt tttttaactt gtaatgcata gcgtaaaacc agaatatctg acatgtggtc
     9241 aaaatgaaga tgagatagga taacaaaaga tatatcttcg atattataac ttttctgaaa
     9301 gttcataaga gttccacttc caagatcaag aattattaac ttattgttta gttcaacaac
     9361 atatccagaa cacggtcttt cagcacttgg atatggaccg ctatttccca acactgttaa
     9421 tttcatcaga tacctccaaa aattctttat ataccctttc attagcatct cgtataccaa
     9481 gagaataagc tttttttgat atttctatta gtctttgatt attattaagt aaactataaa
     9541 ttttctcata caaagcatgt gatgttaagg tttcgttatc atacattatc tcacaagctc
     9601 cattattata aatcatttgt gcattttttt cttgtgcatg atctggtaca taaggagatg
     9661 gtataactat agcaggttta cccatagcaa gtatctcgct tatagatatt gcaccaccac
     9721 gaataaccat taagtcagct tttgcatata tagtatttct ttcataaata tagtcatgaa
     9781 tcataacatt gttcagttta tttattactt tacttactac ttgctgaaaa tacttttttc
     9841 caggagcaaa tattagatta aaatcatcct tattcatgtg atcatttatc attccgataa
     9901 ctgcattatt aattgtcatt gcaccttgac taccgcctat tattactata gtctttttac
     9961 cttctgttgg tttatacttt tccattgaag aaaagcaatc atcaataaga ttcccagtaa
    10021 gtacagtttt ttttgatttg atgaaaaatt ttgaagctga ctcataactt gtcattgcta
    10081 catctgaata tctgcttaac attctattgg ttacacctgg ataagcattc tgttcatgaa
    10141 tcattatttt tgatttatga agatatgctg caaaaatagc aggtcctgat acaaatcctc
    10201 ctgttccaat ggatatgtca ggtttaaatt cacgaacaat tttattcgct tgaaaaaatc
    10261 cttttatcat tataaaaact gttttaatgg atttaaatgt tagaggtctt ttaaaccctt
    10321 caatttctat aaatttaata tcaaagcctt ctcgtggcac aaggtccttc tcaagacctt
    10381 tttgtgatcc aataaataaa atttttgaat caggtttgtg ttttacaatc tcctttgcaa
    10441 ttgatatagc tggatttata tgtcctgcag aacctccgcc tgcaatcaga atcttcatac
    10501 attcaactcc cttttatcta cagattgttt agaaatattc aacaatatac cgatttgcgt
    10561 taaaaacatt accagactgg ttcctccaaa actgaaaaat ggaagtgtaa tacctgtatt
    10621 cggaaccagg cccgttacaa cagcgatatt tagagcagct tgaataaaga ttaatgaagt
    10681 aattccagta gcaagaagtg ttccaaacat atcttcacaa tatattgcta tccttatccc
    10741 tctccataac aacatacaaa acatcgcaat tactaaaaat acaccaatat atcctaattc
    10801 ttctgcaaga atagcaaata taaagtcatt ttgcggttct ggaatatata aaaatttctg
    10861 tgtgctctta ccgagtccga ctccaaaaag cccccccgag cctattgcat atagcgactg
    10921 tattatctga tatcctaaat cagtaggata tgcaaaaggg tccagccaag tcattatcct
    10981 ttctttcatg taatcatagg taaatgcacc aataactgcc aatgtgatta caggtaatac
    11041 agtaagaacg agatatttta tttttgtacc tccaataaaa ataattacga atcctaccaa
    11101 agtgattatt attgcagcac ttaagtgagg ctgaaaaaag attataagtg cagatgataa
    11161 agtgataccc atagttacaa agtatcctat taatggttta tccagtttct taaaatcaag
    11221 aattattttt gagagaaaaa caataaaagc taatttatat atttctgatg gttggaactc
    11281 aaatccgata tttatccatc ttcttgcatc actgtatgct actccaatac caggtatata
    11341 agttaaaatc agtagtatta atcctattgg aaatataatc cagctgagtt ttctatacaa
    11401 tttataactt acactcgaag ctataagcat taatactaat ccaattactg cagcaaacat
    11461 ctggtctttt atgaaataat atatatcgtt aaatctgctt ttcgcataag gagcaccagc
    11521 gctgaaaatc atcaataaac caaaggcggt caatccaagc gcaaataaga aaattacaaa
    11581 atcagtttta tttctgccat tactccttaa cataatccct tgtatgatgc agtcagtggc
    11641 tgttcatact cctcctctaa atcattaaaa atgcaagtat accccctgcg agtgttactg
    11701 ctgtaaatat aataactatc ttattctcgc tccagtgcat aagctcaaaa tgatgatgaa
    11761 ttggagccat taagaaaatc cttttttttg tcctcttata atgcataacc tgtattacag
    11821 aagataatat ttctataaca tatataatgc cgacaattaa tataatccac ggaattctaa
    11881 ggttgattga gattgcagtg actactcctc ctagagctag tgaaccggta tcacccataa
    11941 gtaattttgc aggatgaaca ttaaatacaa gatagcctat catagcaaaa ataactataa
    12001 ttgaaagata ggtcatgttt tcatttttaa aaattatctg cgaagatact gcaaagaaaa
    12061 taaagactgc agaagtcaca gaagcacata acccatcaac tccatcagta aaattaactg
    12121 cattaatgac cgcattgtag aacagaacta taaaaataac atatatccat ccaggaacat
    12181 aaaatatctt tgatgtattt gtaaaaggta ttataatatc tgaaccgata tcagtaaaat
    12241 acacaataaa tattgcatac aatagtgata tcactataaa aaatatcgtt ttaaatatag
    12301 gtttaagacc atctttattc ttctttctta tcttcaagaa atcatcaata aagcctacta
    12361 ttgatgatac tagaattaaa gcacaagaag caagaactag aggatccttc catccgacaa
    12421 ataaggtgca agctaaaatt ggaatgatga atataattcc tcccattgta ggagttccag
    12481 tttttttaag atgttcttta ggaccgtcat ctctaacaac ttgaccaatt ttcatttttt
    12541 ttaatacagg aagcataagt attcctaata taaccgtcaa tacaaaagta acaatagcag
    12601 caattatcat ataatctcct cttcaaatat atccattaac ttttccattg cagaacctct
    12661 tgagccttta agcaatatta catcatcttt ttgtattata tctttaatag tattttttga
    12721 ttcttctatg ctggcatacc tgtaaataca agcgcttctc atgccggaat ctacagctcc
    12781 ttgataataa ttatcagcat ttttgcctat agcaatcaag acatctatat tgttatctgt
    12841 tacaatctca cctgccttaa tatgaaaata cttttcataa tcgcctaact ccagcatatc
    12901 tcctaatata gcaacttttc gcttattgta gtttcccaat aattctaaag atgctttcat
    12961 ggagtcataa ttcgcattat atgcatcatt aatgacagta tatccattaa taacacttat
    13021 ttgggttctc attttctgat tcttgcattc acttattgct ttagatgctt tatcagtatt
    13081 taccttaagt aaatcagaaa ctgcaaatgc gcatagggca ttataaatat tatgaaatcc
    13141 tgggattgaa agattgatat ttttactatt aacagtaaat gaataagtat tatcttcatt
    13201 ttgaacaata ttttgagcaa tataatctgc atcattattg taaatgctaa cttttattac
    13261 tttgtaatta tcagaatctt tgattgatga aagaaaatca tcatcaccat taactacaag
    13321 ataaccatta tcaacataat caagaacctc cgtttttgct ttaaaaatgt tctctttact
    13381 accaagcatg cctatatgac tggttcctat atttgtaatt actgcaattt tgggtttaac
    13441 tatatatgat aactttctga tttcaccaat actattcata cccatttcta taactgctgc
    13501 ttgagtattt tcattgagtg aaaacaaagt cagaggaacg cctgtctgat tatttaagtt
    13561 acctattgtg tagcaggttt tcatttcagt agatattgca gtatttatta attctttagt
    13621 tgttgtttta ccattactgc ctgttatgct tataaatgga atgtcaaaca tatttctgta
    13681 ataccttgca aagtcctgta acgcttctgt tgtatcattt acatagatta catcaccatt
    13741 ataattatct tctttagaac aaacagctgc tgcagcattc aaagaaagtg ctttattaac
    13801 aaaacaatgt ccgtcgaaat tgtctccttt aattgcgaag aataaatcat tttcttttag
    13861 cgttcttgta tcaatagata ctccagtaat gtttttatct atatccttgc aatataagtt
    13921 ttgaaatata ttatatatag aacttaactt tatattcata tttttccttc caaaatacca
    13981 aatataactt ccctttcatc ataatgtgtt ttcactccca taacatcaag atatgtctca
    14041 tttcctttac cggcgagtat cactatgtca tctttttttg ctatcttcat cgcatatcta
    14101 attgcttctg ttctatcagt tatacatatg tattgaccat tagtctgttt tattccgtct
    14161 tcaatcatac taataatttt ttcaggcttc tctgtcctgg aattgtcaga agttattata
    14221 gtaaaatcag aattttcacc tgaaatttta cccatcattg gtctcattga tttatctctg
    14281 tcacctccac aaccgaataa agttataatc cttcctgatg tttttgtttc tcttactgcc
    14341 tgtagaatct ttaacaggct atcaggagta tgtgcataat caatcatcat agtataatct
    14401 ttaccaatat ctaccttctc acatcttcct cttacttgaa catcagaaag tccattttgt
    14461 atgtgaatta tgtctacatc atttaataat gctgctgtaa tagcaaataa actgttataa
    14521 atattaaacc gtccaggtat atcaatcaag acatcaaagc acttattcag ccctttgtat
    14581 gtaaaactgc ttttatcatt tttataaact atattttcag cgataatatc agctttttta
    14641 tctataccga ttgatattat tctgttatct gtttttatat aatgatcact aactaaatat
    14701 tcattaagtg ctttttccac attctcatca tcaatgttta ccaacattac tttacttttt
    14761 tcaaaaatct tttttttaga attatagtaa tcttcaaatg taggatgctc tctttgagtt
    14821 atatgagctt gtgtaagatt agtgtacatc cctatattaa aatgtaaccc atatactctg
    14881 ttcatcttca ttgaaattga tgaaacctcc attatgcagt tagatatttt ttcatttctt
    14941 atatcggaaa aaatttgatt tagctcgtat gatgaaggtg tagtcgtatt tgtcacaatt
    15001 aatttattat tgtaacttat accaatagtt ccaattacag cgctttttaa atctgcttta
    15061 tcaaatatat ttttaatcat ataagaaata gtagactttc cttttgtacc tgtaatacca
    15121 atgatattca tatttttgga tggataatca aaaaacaaag ccgatgactg tgccaaaaat
    15181 attcttgaat cttcaactaa tatagttgct acttgtggat atttacttct gtaaaaatca
    15241 tattcctttg tgataacaat agcagcaact cccttttgaa tcgcattatc tatgtatttg
    15301 tgaccgtctt cgtctgcccc atcaatcgca acaaaaatat catttttttt tgtttttctt
    15361 gaatcaaatg aaattcctga tattataaca tcctttaaat gagatgcttt gatataatct
    15421 gttttagaca ataattgatg tatatccata tactaccttt ctgctaatca agataatctg
    15481 tatatctgaa tgtaatattc acaatagttc ctttttctac atatgctcct gcaggatact
    15541 gtactgaaat acatgttcca ttaccatcaa cattaacgtt aagcccaaga tctttcaatg
    15601 tttttataac ttcctgctcc tgctttccaa taagatacgg aattcttaca gttttcttat
    15661 tctcaggcac atatgtataa agtattataa caccatcttg gttaatttta gttccgcttt
    15721 ctggatattg gtacataatt gttacatctt ctccagctgt acttccttct atttggtaac
    15781 ttaatccata actcttcaat ttttgagttg ccttttcaac attaaaaccg ttcagattag
    15841 gaacataaac tgaatttaca ttattaacaa ctccatcttt ttctacccca agaactccta
    15901 aagattcctc aaatactaat tttgcataag gtgctgcaac ctgacttccg taataagaat
    15961 caccttgagg attaaataca atgaataaaa cagttataac tggattatcg gcaggagcga
    16021 aaccaataaa tgatgatata taaacatcat ctacaatagt ttcacttgtt cctgtttttc
    16081 cagcaaccct atatccttca acataagcat tcttacctcc tccttctttt acgactccct
    16141 caagaaccat tctcaactga tctgaggtat cctgggaaat aacttgccta accacttttg
    16201 gttcaaaatc gtaaacagta ttaccttgct tgtcaataac agcatctgtc aaactgggtt
    16261 caatcaaata accgccattt gcaatagcat tataagcgga gaccatttgt ataggtgtaa
    16321 tttgaaatct ctgtccaaaa gaagcaactg acatatccag ttttgttggt tcatcatgaa
    16381 aaatacttgt tccttctcct atgagctcta ttcctgtatt atctttaaat ccaaaagctt
    16441 cgacatactc ataaaaagtc tttattccga taagatgcga aattttaaca aaaacaggat
    16501 tacatgagtt atatactgct tgaagaaatg tttctgttcc atgaggatat tctttagacc
    16561 agcaatgtat tgttgagccg aatactgtta caggaaagtc atcgacaata gtatcaagtt
    16621 caactatgtc ctcttctagt gccattgctg ttgtaataga tttaaatgtt gaaccaggct
    16681 catatgtatc catgagtgct ttatttcgcc aaactgtact atacagaagc tgaacatcag
    16741 aagaacgaag tccagtccac ccttctattg tgtatccttc aggtaataat cgaggattat
    16801 ttaaatcaaa tccaggacta gataccattg caagaatttc actagtctta ctgtttgata
    16861 ctatacaaac tgcaccatct tcagacttat tgtcttcaat tgcttgttct actatctttt
    16921 ctgctattct ctgaatatct gtattaagtg ttgtgattac atcaaatccg tctatggctt
    16981 ctattcgtga ttcaaaagca aatggcaatt ctgtacctgc aacatctagt tctgtcaaaa
    17041 tcttaccatc agtgcctttt aaatacttat ctaaagaaag ttccaaacca aataatccct
    17101 gattatcatc acctgcaaat ccgattgcat gtgaagcgag gc
//
LOCUS       DAOSXM010000043        17078 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2111
            NODE_2115_length_17078_cov_8.558245, whole genome shotgun sequence.
ACCESSION   DAOSXM010000043 DAOSXM010000000
VERSION     DAOSXM010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864178
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 17078)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17078)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:24:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,261
            CDSs (total)                      :: 2,229
            Genes (coding)                    :: 2,218
            CDSs (with protein)               :: 2,218
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 3 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17078
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2115_length_17078_cov_8.558245"
                     /isolate="AMR_MDS_2111"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..433
                     /locus_tag="PLQ68_06690"
     CDS             <1..433
                     /locus_tag="PLQ68_06690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HXK72375.1"
                     /translation="YTIFNTLIFSVQLSVAYPYCSDAVYEKLSQKPKLIYKDNQTLIS
                     GVLIRHLVLPGLYKDSIDILNWIKNLLSTNNFECLISIMSQYTPYHQSNLYPEINRRI
                     TGFEYKKVINEANRLELNGYMQERISSKDSYIPDFDFEGII"
     gene            complement(426..1547)
                     /locus_tag="PLQ68_06695"
     CDS             complement(426..1547)
                     /locus_tag="PLQ68_06695"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HXK72376.1"
                     /translation="MRWTRKEYLDYVTFKSNEKSMIVELFGPLIGLEEEWKTQGATED
                     ELNLTAFDWDYVDNINCGANFDVFDAPEERVIEETKEHIITIDALGRKMKLMKASATI
                     PLPMDYPVKNMDDWIKIKHFYEFNENRIDYNHVKLAIKKQTEGVLVKACVPGGFDLPR
                     QLMGEEELCYAYYEQPELIKDILDTASETAIKCCERLAKHITVDHLAIHEDMAGKSGS
                     LIGPNHINEFLKPYYLRIWDAFKNNGCRVFVQDSDGNMNSVIDSFLGCGLTQMYPMEP
                     AAGMDIVKVHNKYGNKLSLKGGIDKFILRENKLAVLRELEYKMQPSMRQGGIVFGFDH
                     RIPNGSSLELYRYYVKTAREILGREPLDKKNKGWMRQAI"
     gene            complement(1586..2545)
                     /gene="holA"
                     /locus_tag="PLQ68_06700"
     CDS             complement(1586..2545)
                     /gene="holA"
                     /locus_tag="PLQ68_06700"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HXK72377.1"
                     /translation="MKYEDIKNEISQSVFKQIYLLYGDDEFLKNSVYNVIKSNLLKKN
                     NNKLDELKYNDESDIFDILNECNTYSFTGNAKLIKCKNTNFFSKEEYTEPLTRIVNDI
                     SDNTFIIFIETNISKKLTSFKNFNKSGYAYDISKGKPDDVKKFIYLSFKREGKTISSS
                     DLDLFIEFSGLNLAYISLNIDKILLYMNDQKNVTSDMIKLLCNGITDVKSYELCSYLC
                     KKDLKNTLNVYYDMIALKYSIPYFLAILFNTFYELYNIKISNTLKSDDFRKRIAIDNA
                     SKFSLEALNTIIKDICDFDHDFKTGKINQDSLMIILFSKIVNS"
     gene            complement(2565..4880)
                     /locus_tag="PLQ68_06705"
     CDS             complement(2565..4880)
                     /locus_tag="PLQ68_06705"
                     /inference="COORDINATES: protein motif:HMM:TIGR00361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HXK72378.1"
                     /translation="MPFLAIFSITGIYTGTSYEKITSYILFLLLLLFYLYINIKENKY
                     SIKYKIAVTSFLVLFFIFFYIYSSVYFSAIDNNNIHNDKKSIRIIGYVKNLNEINEKE
                     LKFTINCTDGFNYNVVLSDINDNSSINTGDIYIIEGKLYKPQKATNKGQFDYQKYCYS
                     KNVTATIYTDYENITNIGNKTILYKIGILRNKAVKRASMNLSKQEKNISVALITGGSE
                     QIDDEIDLLFRKSGLSHLLSISGTHFSILILPMALLSILLFRRKYINFIIFTCLIIIL
                     LVFTGMKIGAIRSAICFICIAVCKTFFYDAKKSSVISIALVLILVINPLSIYNTGFIL
                     SFGSVVSILLFSKKITNCILKLTKYCSSLLEKILLSKYVMLKGISIRINNKKHKTVLK
                     YLFSGLSLIISVQPAILILSYKLFHVIYPYSLISNIIIFPIIPVLVLSIWLSILSPQI
                     FNPVFSVCLKIIMKVAEWVANFSYSQIYLKDISTISIILIVASLVIYKMKFKNNIVTK
                     NLLVSFFLILSVICFIPNICTKMIFFDVGQGDSALFCSGKGTNILIDTGKYIDPSCIA
                     YYTGQRLDIVIITHPDNDHCGGLFEILDNLNVKKVILPKSNHIKNLTFIEDLKVNYKN
                     IDVVQVEENDIIKTDDFIIRVLNPEISKEYSDINNASIIFSLVTKQCKFLMTGDADIT
                     NIHVNELYKTDILKIPHHGDDKVINRNVLSYINPDISIISVGKNNTYGHPGKKTLAIL
                     DLLNLKYLRTDVNGSIQIYLIFDKYMVYKYQ"
     gene            complement(4983..6644)
                     /locus_tag="PLQ68_06710"
     CDS             complement(4983..6644)
                     /locus_tag="PLQ68_06710"
                     /inference="COORDINATES: protein motif:HMM:NF024716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4091 domain-containing protein"
                     /protein_id="HXK72379.1"
                     /translation="MKYSAKIISSLEKCFINDDITLLKSINNLSCLKNEKLNFQIAYT
                     VYSDTLRDKKPAFFMIKSELNEFIKIYKVESVPCRFPVMHNTKELDYQKKEPYLYPDL
                     LDEYIHSDNVYFVPNVLQSLFIEINLDYNSLFHGEYTLECIFFDSDNEILYKTDINIE
                     IINSYLYDEKIKYTQWFHSDCLSSYYNVKPLSEKHFKIIDNFMKTASEYGINTILTPV
                     LTPPLDTKIGGERPTIQLIGIRKSDLGYEYNYDLLDKWINLCRKNNIEFFEISHFFTQ
                     WGAKHAPKIIATVNGRKKRIFGWDTDSTSDDYVAFLRDFIKNLIIHLKKLNIDKKCIF
                     HISDEPNEKTVDNYKAAKNTISDLLEDYMVTDALSSIEFYKQGIVKKPIPSTDKIHDF
                     IELKVKNLWAYYCGSQYIDVSNRYIAMPSYRNRIIGAQLYKYDIEGFLHWGYNFYYNQ
                     YSRKLINPFLITDADFFAPSGDAYTVYPGNDYNPLKSLRLLIFNEALQDIRAFKTLEK
                     LTSYSHVISIIEEEIDELTFNNYPRNNDYILNLRKKINREISQHT"
     gene            complement(6641..>7029)
                     /locus_tag="PLQ68_06715"
     CDS             complement(6641..>7029)
                     /locus_tag="PLQ68_06715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="mucin desulfatase"
                     /protein_id="HXK72380.1"
                     /translation="VMPGSILYDYGDALRISGSSAAEDEQDLNKVHFVIDNFVAFTKG
                     FISSMKDSITQCELSLLPFSIKLITMEIGMRFLTDYLNNDIYFAIHKKDHNLYRARTQ
                     FKLVSEIEYNIEKMNKIVYDIIREGV"
     assembly_gap    7030..7129
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7130..7890)
                     /locus_tag="PLQ68_06720"
     CDS             complement(<7130..7890)
                     /locus_tag="PLQ68_06720"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HXK72381.1"
                     /translation="MTNEFEIANNFSYRGNITDISPLGAGHINKTYVVTTDINKYTLQ
                     KINTAVFKDPVGLMENIFNVTEFLKKKISDRGGDNKRETLSVIKTKTDSLFYVDSNNE
                     YWRSYLFIDDVITYQEPKSLKVIYESGFAFGDFLNLLSDYDMSTLNDTIKDFHNTPKR
                     FDNFKKALQDDVANRALNVTDEIEFLLKREEDCHLIINRINDDTIPLRVTHNDTKLNN
                     LLFDKYTDKPICILDLDTVMPGSILYDYGDALRISG"
     gene            complement(7899..8666)
                     /locus_tag="PLQ68_06725"
     CDS             complement(7899..8666)
                     /locus_tag="PLQ68_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004630971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HXK72382.1"
                     /translation="MLIKSFKKDNLDVNIFDNRSNMGKKAANDVKEAIRKALTQKEEI
                     NIIFAAAPSQNEFLEALISYNDIDWERINAYHMDEYLGISEDAPQGFGNFLKNAIFSK
                     VNFKSINYLNPSCDDVTKECSRYEELLINNQIDIVCMGIGENGHIAFNDPPVADFSDK
                     FKVKIVKLDEKCRNQQVNDGCFDNIDKVPKYAMTLTIPALFEAKKLFCMVPAQSKADA
                     VYDTLNGEIKETCPASILRLHLSAKLYLDKLSASRIM"
     gene            complement(8761..10080)
                     /gene="eno"
                     /locus_tag="PLQ68_06730"
     CDS             complement(8761..10080)
                     /gene="eno"
                     /locus_tag="PLQ68_06730"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HXK72383.1"
                     /translation="MKQYIAIEAVWAREVLDSRGNPTVEVEVYVEGGFVGRAAVPSGA
                     STGAFEAVELRDGNKKIYLGKGVEKAVENVNEIIAPELEGLNVLDQVYIDKLMIQIDG
                     TENKGKLGANAILGVSLAVAKAASEALGVSLYEYIGGVNSKVLPVPMMNIINGGKHAD
                     NSVSCQEFMIMPVGASSFKEALRWCAEVFHTLKKVLKDKGYSTAVGDEGGFAPNLKTD
                     EEALQVIVEAIKAAGYKPYDQIRIALDPAATEMYEEAKAKGKEGCYYFWKTDRMFTVD
                     ELIDFWAEMCDKYPIISIEDGLAEEDWEGWKKLTERLGSKIQLVGDDLFVTNTKRLKK
                     GIELGVANSILIKVNQIGTLTETLEAIEMANRAGYTAVTSHRSGETEDTTIADIAVAT
                     NSGQIKTGAPSRTDRVAKYNQLLRIEEELDDIAVYPGLDAWFNIKSK"
     gene            complement(10094..10849)
                     /gene="tpiA"
                     /locus_tag="PLQ68_06735"
     CDS             complement(10094..10849)
                     /gene="tpiA"
                     /locus_tag="PLQ68_06735"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010251556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HXK72384.1"
                     /translation="MSRKKVIAGNWKMNKTPSETSIFITELSDRVKQTKDIVICAVPY
                     VCLTAALKSAKRTKIKIAAQNLHFEDNGAFTGEVSTDMLTDLGVKYVIVGHSERRQYY
                     NETDATVNKKILKALKAGLKPILCVGESLDQREMGVTSDFVRYQVRYALNGVSSEDMK
                     NVIIAYEPIWAIGTGKTATNEQANDVCKTIRETLVELYGRRVANAALIQYGGSVNVKN
                     ANELFNMSDIDGGLVGGASLKIDDFDKIVNYNK"
     gene            complement(10846..12036)
                     /gene="pgk"
                     /locus_tag="PLQ68_06740"
     CDS             complement(10846..12036)
                     /gene="pgk"
                     /locus_tag="PLQ68_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HXK72385.1"
                     /translation="MASLNKKTIEDINVSGKRVIARMDFNVPLKDGVITDDKRIREAM
                     RTIRYLSENGAKTILVSHLGRPKGEFNMKYSLGPIAERITSLLGKKVVLAEDVIGPDA
                     KSKAASLKDGDVMLLENVRFHAEEEKNDPAFAKELASMAEIFVNDAFGTAHRAHASTA
                     GLADYLPAVCGYLIKKEIDIMGKALKDPERPFVAILGGAKVSDKIGVIESLLDKVDTI
                     LVGGGMAYTFDKAKGYEIGTSICELDKIGLADELIKKAEKKNVKLLLPLDSVVADSFS
                     NEANKKTVDCCSIPDNMMGMDIGEKTIASFTEEILKAKTIVWNGPMGVFEFENFANGT
                     RQIAKAVAESGAVSIIGGGDSAAAVEILGYADKVTHISTGGGASLEFLEGKVLPGIDV
                     LEDK"
     gene            complement(12085..13098)
                     /gene="gap"
                     /locus_tag="PLQ68_06745"
     CDS             complement(12085..13098)
                     /gene="gap"
                     /locus_tag="PLQ68_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010251552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HXK72386.1"
                     /translation="MTVKIGINGFGRIGRLVFRAAINNPDVEIRGINDPFLDVPYMAY
                     MLKYDSVHGQFKGTIETGDDYLVVNGKKVKVYTSKDPAEIGWKECGAEYIVESTGVFL
                     TKEKAQGHLTAGAKKVVMSAPSKDDTPMFVMGVNQDKYTKDMTFVSNASCTTNCLAPL
                     VKIVHDNFGVVEGLMTTVHSTTSTQKTVDGPSNKDWRGGRAAATNIIPSSTGAAKAVG
                     KVIPSLNGKLTGMSMRVPTIDVSIVDLTVRLDKPASYEAIKSAVKNASENELKGIVGY
                     TEDDVVSTDFIGDSRTCIFDAKAGISLNDNFVKLLAWYDNEWGYSNKVVDLILHMAKV
                     DAK"
     gene            complement(13209..13694)
                     /gene="trmL"
                     /locus_tag="PLQ68_06750"
     CDS             complement(13209..13694)
                     /gene="trmL"
                     /locus_tag="PLQ68_06750"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HXK72387.1"
                     /translation="MINIVLVEPEIPQNTGNIARTCAATGARLHLVRPLGFNITDSKL
                     KRAGLDYWNDVDIFYYDSLDNFMDIHKNDNLYFSTTKACSLYTEIKYPKDVYILFGKE
                     TKGLPETLLESNLNKTIRIPMNSNIRSLNLSNSAAIITYEILRQLGFETMQLYGKYGN
                     S"
     gene            complement(13764..13964)
                     /locus_tag="PLQ68_06755"
     CDS             complement(13764..13964)
                     /locus_tag="PLQ68_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010247209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HXK72388.1"
                     /translation="MNRVTKDMIIIDVLKMDPNTADIFFEYGMHCIGCPHSSGESIAQ
                     ASAAHGVNSDALVKALNEYFEQ"
     gene            complement(13961..15172)
                     /locus_tag="PLQ68_06760"
     CDS             complement(13961..15172)
                     /locus_tag="PLQ68_06760"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003867136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006102 - isocitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HXK72389.1"
                     /translation="MSKIIMTNPIVEIDGDEMTRVIWTLIKDHLLVPNIELNTEYYDL
                     SLDNRDKTDDSVTVECANAIKKHKVGVKCATITPNAQRMKEYTLKKMWKSPNGTIREI
                     LDGTVFREPVLVNNIKPTVSCWKKPIIIARHAYGDVYSAKEYQVKGPGILSAEYVYND
                     GTTKNIEGFNFDGPGIIQLMHNKDSSISSFARSCFNFAIDRKMDLWFSTKDTISKKYD
                     HHFKDIFQSIFENEYKKNFDNLGIEYFYTLIDDAVARVVRSEGGFIWALKNYDGDVMS
                     DMVSAAFGSLAMMTSVLVSPNNEFEFEAAHGTVTRHYYKHLKGEETSTNPIATIFAWT
                     GALRKKGEMDSNIHLIDFSNKLEKSCLESIEQGYITKDLSRLIESDKITVMNTESFII
                     NIAERLKEKLI"
     gene            complement(15165..15689)
                     /locus_tag="PLQ68_06765"
     CDS             complement(15165..15689)
                     /locus_tag="PLQ68_06765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase C-terminal domain-containing
                     protein"
                     /protein_id="HXK72390.1"
                     /translation="MIELFTPFSEEILCALKPFDKVLITGKMYIARDQAHKRMFEVGI
                     PIDIKNQCIYYCGPSEPKKGQIIGSAGPTSSYRMDKYTPYLLDKGLKAMIGKGKRSNF
                     VIDSIVKNKCIYFGAIGGLGARLSKCIKKQSIIAYDDLGTEALREIYVEKFPAIVIIN
                     CIGNSIYESKVTNE"
     gene            complement(15686..16528)
                     /locus_tag="PLQ68_06770"
     CDS             complement(15686..16528)
                     /locus_tag="PLQ68_06770"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF017493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HXK72391.1"
                     /translation="MIRTIKSDDIINEISRACIECACNLDKKAYSIYKKCIDVETNEN
                     AKYVLSELIYNADIASNENIPLCQDTGMVICFAEIGINVHLEEPLQSSIDKGVRKGFA
                     DGYLRKSIVNDPFNRINTQDNTPSAIYTDIVDGSELKINLMLKGFGSENMGFVKMFNP
                     SEDVNVIINYLSDKIISNAVNACPPIFVGIGIGGTMEMAALLSKKVLFRPIDQNNKDS
                     FYSAMEQKIKDNINNSNVGPSGYGGKTTVISVAIEKYPTHIAGLPVAVTIQCHALRHK
                     EIVI"
     gene            complement(16503..16796)
                     /locus_tag="PLQ68_06775"
     CDS             complement(16503..16796)
                     /locus_tag="PLQ68_06775"
                     /inference="COORDINATES: protein motif:HMM:NF012442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HXK72392.1"
                     /translation="MNRNELSIRLANEKKITKLQSDDIINTVFNIIKEALKEEQKVCI
                     KDFGTFYVKPRKGRQLKDISTNRIIKVPDLMELKLKSSQAMKDYLNDKNDKIR"
     gene            complement(16815..>17078)
                     /locus_tag="PLQ68_06780"
     CDS             complement(16815..>17078)
                     /locus_tag="PLQ68_06780"
                     /inference="COORDINATES: protein motif:HMM:NF013347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HXK72393.1"
                     /translation="FKDLQPPDEKGIIICNPPYGQRLEDIETAKQIYSDMPKAFSSFS
                     KWSKYILAPEKIFELSYGKQADKRRKLYNGKILCNLYQYFGSK"
BASE COUNT         5429 a         2944 c         2149 g         6456 t
ORIGIN      
        1 ttataccatt ttcaataccc ttattttttc tgtccaatta agtgtagcct atccatactg
       61 ttctgatgca gtgtatgaaa agctttctca aaaaccaaaa ttaatatata aagataatca
      121 aacattaatt tccggtgtgc ttataaggca tttagtgctt ccgggtttat ataaagattc
      181 tattgatata ttgaattgga taaaaaactt acttagtaca aataattttg aatgtttgat
      241 aagtattatg agtcaatata ctccgtatca tcaaagcaat ttgtatcctg aaattaatag
      301 gaggattact ggatttgagt ataaaaaagt tataaatgaa gcaaatagac ttgaattaaa
      361 cggatatatg caagaaagaa tctcatcaaa agattcttat attcctgact ttgattttga
      421 aggtattata tagcttgtct catccagcct ttattctttt tatcaagagg ttctcttccc
      481 aatatctctc ttgccgtctt cacataatat ctgtacaatt ccaaagatga accattaggt
      541 attctgtgat caaatccaaa tactatccca ccttgtcgca ttgaaggctg catcttgtat
      601 tccaattctc taagaactgc aagcttattt tctcgaagaa taaatttatc tattcctcct
      661 ttaagagata atttattacc atacttatta tgtactttta cgatatccat accagctgct
      721 ggttccattg gatacatctg tgttaatcca catcccaaaa atgaatctat tacagaattc
      781 atatttccat cagaatcttg aacaaaaact ctacatccat tattcttaaa agcatcccaa
      841 attcttagat aatatggttt tagaaactca ttaatatgat taggaccgat aagtgatccc
      901 gactttcctg ccatatcttc atgtatagct aaatgatcaa cagttatgtg tttagctaat
      961 ctttcacaac attttatcgc agtttctgat gccgtatcaa gaatatcttt tattagttct
     1021 ggttgctcgt aatatgcata acataattct tcttcaccca ttaattgtct tggaagatcg
     1081 aaaccacctg gaacacatgc ctttactaaa actccttctg tttgtttttt tattgcaagt
     1141 ttaacatggt tatagtctat cctattttcg ttaaattcat aaaaatgttt aattttaatc
     1201 cagtcatcca tatttttgac aggataatcc attggcaaag gaattgtagc tgaagccttc
     1261 atcaatttca tttttctacc gagagcatct attgtaatta tatgctcttt tgtttcttca
     1321 atcactcttt cttctggagc atcaaataca tcaaaatttg ctccacaatt tatattatca
     1381 acataatccc aatcaaatgc agtaagattt aattcatctt cagtagctcc ttgagttttc
     1441 cactcctctt caagtcctat caatggtccg aataattcta ctatcatact tttttcgttt
     1501 gatttaaatg ttacataatc cagatattct tttcttgtcc aacgcatatc tacacctctt
     1561 gcttttgatt atagcatagt taaatttaac tattaactat ttttgaaaat aaaattatca
     1621 ttaaagaatc ctgattaatt ttacctgttt tgaaatcgtg atcaaaatca catatatcct
     1681 ttattattgt atttaaagct tccaaactga attttgatgc attatcaata gcaattcttt
     1741 ttctgaaatc atcagattta agagtattac taatttttat gttgtataac tcatagaaag
     1801 tgttaaataa tattgctaaa aaatatggta ttgaatattt taatgcaatc atatcataat
     1861 atacattcaa tgtgttcttt aaatccttct tgcataaata tgaacataat tcataagatt
     1921 ttacatcagt aataccgttg caaagaagtt ttatcatatc tgatgtaaca tttttttggt
     1981 cattcatgta taacaggatt ttatcgatat ttaatgatat gtatgcaagg ttaaggcctg
     2041 aaaactcaat aaataaatct aaatcagaag aagagatggt ttttccttct cttttgaaac
     2101 ttaaatatat gaatttttta acgtcatctg gttttccttt tgatatatca tatgcatatc
     2161 cagatttatt aaaattttta aatgaggtta gtttcttgct tatatttgtt tcaatgaaaa
     2221 taataaatgt attatcagaa atgtcattta ctattcttgt taagggctca gtatattctt
     2281 ctttcgaaaa aaaattcgta tttttgcatt tgattaattt tgcatttcca gtaaatgaat
     2341 atgtattgca ctcattgaga atatcaaata tgtctgactc atcgttatat tttaattcat
     2401 ctagtttatt attattcttt tttagcaaat tactttttat tacattatat actgaatttt
     2461 taaggaattc atcatcacca tatagcagat atatctgttt gaaaactgat tgcgatattt
     2521 cattttttat atcttcatat ttcataaatg cattctatca tatttcattg atacttataa
     2581 accatgtatt tatcaaaaat caagtaaatt tgaatacttc cattaacatc agttcttaaa
     2641 tatttcaaat ttaataaatc taaaattgct aatgtttttt tacctggatg cccgtatgta
     2701 ttatttttac ctactgaaat aatagaaata tctggattta tgtacgatag tacatttcta
     2761 tttattactt tatcatctcc atggtgagga atttttagaa tatccgtttt atatagttca
     2821 ttcacatgaa tattcgtaat atcagcatca ccagtcatta aaaacttaca ttgctttgtt
     2881 actaaactaa atattatgct agcattattt atatcactat attccttact aatttctgga
     2941 ttcaaaactc gtataataaa atcatcagtt tttataatat cattttcttc tacttgaacg
     3001 acatcgatat ttttataatt taccttcaaa tcttctataa atgttaaatt ttttatatga
     3061 ttggattttg gaagaattac tttctttaca tttagattat ctaaaatttc aaataaacca
     3121 ccacagtgat cattatctgg atgagttatt ataactatat ctaatctttg ccccgtataa
     3181 taagctatac atgaaggatc aatgtatttg cctgtatcaa taagtatatt tgttccttta
     3241 cctgaacaaa ataaagcact atctccctgt ccaacatcaa aaaaaatcat tttagtgcat
     3301 atattaggta taaaacaaat aacactcaaa attaaaaaaa agctaactag taaatttttg
     3361 gttactatat tatttttaaa tttcatttta taaataacta atgatgctac aatcaagatt
     3421 atgcttattg ttgatatatc tttaagatat atctgggaat aagagaaatt tgcaacccat
     3481 tctgcaactt tcattataat ttttaagcat acagaaaata caggattgaa tatctgtgga
     3541 gacaaaattg aaagccatat agataagact agtactggta ttataggaaa tattattata
     3601 tttgaaatca aagaataagg atagatgaca tggaataatt tatatgaaag tattaaaata
     3661 gctggctgaa ctgatattat taaagataat cctgaaaata ggtattttaa tactgtttta
     3721 tgctttttat tatttattct aatagatatt cctttaagca ttacatactt tgataataaa
     3781 attttctcaa gtaatgagct acaatatttc gtgagcttca aaatacaatt tgtaattttt
     3841 ttgctgaata ataaaatact aactacagaa ccaaaagata gtataaaacc tgtgttatat
     3901 atagataaag ggttaattac caatatcaaa acaagagcga tgctaatgac agacgacttt
     3961 tttgcatcat aaaagaaagt tttacataca gctatacaaa taaaacaaat tgcagatctt
     4021 attgcaccaa tcttcatgcc tgtaaataca agtaatatta taatcaaaca agtaaaaatt
     4081 ataaaattta tatacttcct tctaaataaa agtatagaga gtaatgccat aggcaatata
     4141 agaatactga agtgtgtacc ggatatactt agtagatgtg ataatccgct ttttctgaat
     4201 agtaaatcaa tttcatcatc tatctgttca gaacctcctg ttattaatgc aactgatatg
     4261 tttttctcct gtttagaaag attcatactt gctcttttaa cagctttatt gcgtagaatt
     4321 ccaattttgt ataatatggt tttgttccca atattagtta tattctcata atctgtatat
     4381 attgtagcag tcacattttt tgagtaacaa tatttctgat aatcaaattg acctttattt
     4441 gttgcttttt gaggtttata tagttttcct tcaataatat aaatatcacc tgtgttgata
     4501 cttgaattat cattaatatc agataaaact acattgtaat taaatccatc tgtacaattt
     4561 atagtaaatt ttagttcttt ttcatttatt tcatttaaat ttttcacata ccctataatt
     4621 cttatacttt ttttatcatt atgaatatta ttattatcaa tagcagaaaa ataaacagat
     4681 gaataaatat agaagaatat aaaaaacaga acaagaaaag atgttacagc tattttatat
     4741 tttatcgaat atttattttc cttgatattt atatacaaat agaaaagcaa tagtaaaaga
     4801 aacagtatat atgatgttat cttctcatag gaagtacctg tataaatacc tgtaatagaa
     4861 aaaatggcta aaaaaggcat aaatgacttt ttgattatca ttcaaccttt ataatctaat
     4921 aatcagtatt attacatata caaaagaagt aaaattacta gattttagat tttcttgata
     4981 tcttaagtgt gctgactaat ttcacgattt attttttttc ttaaatttaa aatgtaatcg
     5041 ttgtttcttg ggtaattatt aaatgtcaat tcatcaatct cttcttcaat gatactaata
     5101 acatgactat atgaagttaa tttctctaaa gttttaaatg ctcttatatc ttgtaaagct
     5161 tcattaaaaa ttagtaatct taaagattta agcggattat agtcatttcc tggataaaca
     5221 gtatatgcgt ctccacttgg tgcaaagaaa tcagcatcgg taattaaaaa aggattaatt
     5281 aattttcttg aatactggtt ataataaaaa ttatatcccc aatgtaaaaa accttctata
     5341 tcatatttat ataactgtgc accaataatc ctgtttctgt aggaaggcat agcaatatat
     5401 ctgttgctta catctatata ttgactacca cagtaatatg cccacaaatt ttttactttt
     5461 aattcaataa agtcatgtat cttatctgtt gaaggaatag gttttttaac aattccctgt
     5521 ttataaaact caatagaaga taatgcatct gtaaccatgt agtcttcaag caagtcagaa
     5581 atagtatttt ttgctgcttt gtaattatca actgtttttt cattaggttc atcagatata
     5641 tgaaatatac actttttatc tatgttaagt tttttaagat gaataattag atttttaata
     5701 aaatcacgta agaatgcaac atagtcatcc gatgtagaat cagtatccca tccgaatatt
     5761 ctcttttttc ttccatttac tgtagctata atctttggtg catgtttcgc tccccattgt
     5821 gtaaagaaat gagatatttc aaaaaattct atgttatttt tcctgcataa gttaatccat
     5881 ttatcaagaa gatcataatt atactcatat cctaaatcac tttttcttat gccaataagt
     5941 tgaattgtag gtctttctcc tcctattttt gtatctaaag gaggtgtaag aacaggagta
     6001 agaattgtat taataccata ctcgctagca gtcttcataa aattatcgat tatcttaaaa
     6061 tgtttttctg ataaaggctt tacattgtaa taagaagata aacagtctga atgaaaccac
     6121 tgtgtgtatt tgattttttc atcatataaa tatgaattta ttatctcgat atttatgtct
     6181 gttttataaa gtatctcatt atcactgtcg aaaaatatgc attccaaagt atattctcca
     6241 tgaaataatg aattataatc aagattgatt tctataaaga gtgattgaag aacattcgga
     6301 acaaaataaa cattatctga gtgaatatac tcgtcaagca aatctggata aagatacggc
     6361 tctttttttt ggtaatctaa ttctttagta ttatgcataa caggaaatct gcatggaaca
     6421 gattcaactt tgtaaatctt tataaattca tttaattctg atttaatcat aaaaaatgca
     6481 ggcttcttat cacgcagagt atcggaatat actgtatatg caatctgaaa attaagcttt
     6541 tcattcttga gacatgacaa attgtttatt gattttaata atgtaatatc atcattaata
     6601 aaacattttt ctaatgatga tatgattttc gctgagtatt tcatacaccc tcccttatta
     6661 tgtcataaac tattttattc attttttcaa tattgtattc aatttctgaa accaacttaa
     6721 actgagttct tgcacggtat aagttgtgat ctttcttatg aatagcaaaa taaatatcat
     6781 tgtttaaata gtcggtcaga aatctcattc ctatttccat agtaattaat tttatagaga
     6841 aaggaagcaa tgataattcg cattgagtta tactatcttt catagatgat atgaatcctt
     6901 ttgtgaatgc tacaaaatta tcgataacaa aatgaacttt atttaaatct tgttcgtctt
     6961 ctgcagctga tgaacctgat attctcaaag catcaccgta atcatataaa attgatcctg
     7021 gcattacagn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc ctgatattct
     7141 caaagcatca ccgtaatcat ataaaattga tcctggcatt acagtatcaa gatcaagaat
     7201 acatatgggt ttatcagtgt acttgtcaaa aagcagatta tttaatttag tatcattatg
     7261 agtaactctt aaaggaattg tatcatcatt aattctgttt attattaaat ggcaatcttc
     7321 ttctcttttt aaaagaaatt caatttcatc agttacattt aaagctctgt tggcaacatc
     7381 atcttgtaag gcttttttga aattatcaaa tctctttgga gtattatgga aatctttaat
     7441 tgtatcattg agtgtagaca tatcataatc tgataacaaa ttcaaaaaat caccaaaagc
     7501 aaaaccgctt tcatatatta cttttaaact tttaggttcc tgatatgtta ttacatcatc
     7561 tataaataga tatgatctcc aatattcatt attggaatcc acataaaaaa gagaatccgt
     7621 tttggttttt attactgata aagtttctct tttattgtcc cctcctctat ctgaaatttt
     7681 ctttttaaga aactcagtaa cattaaaaat attttccata agacctactg gatctttaaa
     7741 aactgcagta ttaatctttt gaagggtata cttattaata tctgttgtta caacatatgt
     7801 tttattaata tgtcctgcac ctaaaggtga aatgtcagta atatttcctc tatagctaaa
     7861 attattggct atttcaaatt cattagtcat atattacctc acattattct agaagcgctt
     7921 agtttgtcca aatacagctt tgctgataaa tgtaatctaa gtatactcgc aggacatgtt
     7981 tcctttattt cgccatttaa agtatcataa actgcatcag cttttgattg tgcaggaacc
     8041 atacagaata gttttttagc ttcaaacaac gctggtatag ttagtgtcat tgcatatttt
     8101 ggaaccttgt cgatattgtc gaaacaacca tcatttactt gttggtttct gcacttttca
     8161 tcaagtttaa ctatcttaac tttaaactta tctgaaaaat cagcaactgg aggatcatta
     8221 aatgctatat ggccattttc accaattccc atacatacga tatctatctg gttatttatt
     8281 agcaattctt cataacgaga acattcttta gtaacatcat cacaagaagg gttaagatag
     8341 ttaattgatt tgaaatttac ttttgagaaa atagcatttt ttaaaaaatt gccaaatccc
     8401 tgaggtgcat cttcacttat accaaggtat tcatccatat gatatgcatt tattctttcc
     8461 caatcaatat cattatatga aatcaaagct tctagaaatt cattttgtga tggagctgca
     8521 gcaaaaataa tgttaatttc ttctttttga gttaatgctt ttctaattgc ctcttttaca
     8581 tcattagctg ctttcttacc catattgctt cgattatcaa aaatgtttac atccagatta
     8641 tcttttttaa aactttttat caacataatt ttctcctttt gaaatatatg tattgattat
     8701 atatataaaa ataaaaaggc acaatcgttt gactgtgcct tatatggtat ttattgaaaa
     8761 ttacttggac ttaatattaa accatgcatc caaacctgga taaacagcaa tatcatcaag
     8821 ttcttcttca attcttaaca actgattgta tttagcaact ctgtctgttc ttgaaggagc
     8881 accagtttta atctggcctg aatttgtagc tacagctata tcagcgattg ttgtgtcttc
     8941 tgtttctccg gaacgatgac ttgtaacagc tgtataacca gctctgttag ccatttcaat
     9001 agcttctaat gtttcagtaa gtgttcctat ttgattaact tttattagta ttgaattagc
     9061 aactccaagt tcaattccct tcttgagcct tttagtatta gtaacaaata aatcatctcc
     9121 tacaagctga atcttgctac ccagtctttc ggtgagtttt ttccagcctt cccagtcttc
     9181 ttcagcaagt ccgtcttcga tagagataat tggatactta tcacacatct cagcccaaaa
     9241 atctattaat tcatcaactg taaacattct atctgttttc cagaaatagt aacatccttc
     9301 tttacctttt gctttagctt cttcgtacat ctctgtagca gctggatcaa gagctattct
     9361 gatttggtca taaggtttat atcctgctgc tttaatagct tcaacaataa cctgtaatgc
     9421 ttcttcatca gttttaaggt taggtgcaaa cccgccttca tctcctacag ctgtagagta
     9481 acctttatct ttaagaactt tttttaatgt gtgaaatact tcagcacacc atcttaaagc
     9541 ttctttaaag ctggatgctc ctactggcat gatcatgaat tcctgacagc ttacagaatt
     9601 atcagcatgc tttccaccat tgataatatt catcattgga actggaagaa ctttggaatt
     9661 aactccaccg atatattcat aaaggcttac ccctaaagct tcgcttgctg cttttgctac
     9721 tgcaagagaa acacctaaaa ttgcatttgc tccaagtttt cctttgttct ctgttccatc
     9781 gatttgaatc atcagtttat caatataaac ttggtcaaga acatttaaac cctcaagttc
     9841 aggagctata atctcattta cgttttctac tgctttttca acacctttac caaggtatat
     9901 ttttttgttt ccatctctta attcaacagc ttcaaaagct cctgtagatg ctccagatgg
     9961 aacagcggct cttcctacaa aaccaccttc tacatatact tcaacttcta cagtaggatt
    10021 tcctcttgaa tctaaaactt ctcttgccca tacggcttca atagctatat attgtttcat
    10081 tttatgccct ccattatttg ttatagttta caattttatc aaaatcatca atttttaagc
    10141 tggctccacc tacaagtccg ccatcgatat cgctcatgtt gaaaagttca ttagcatttt
    10201 taacgttcac agaaccgccg tattgtataa gtgcagcatt tgctactctg cgaccatata
    10261 attcaacaag agtttctcta atagttttac atacatcatt tgcttgttca ttagttgcag
    10321 ttttacctgt accaatagcc catataggct cataagcaat tattacattt ttcatatctt
    10381 cagaagacac tccgtttaaa gcatatctaa cctgatatct gacaaaatcg cttgttacac
    10441 ccatttctct ttggtcaagg ctttcaccaa cacaaagaat aggttttaaa ccagctttta
    10501 gtgctttaag tatttttttg ttaacggttg catctgtttc attataatat tgtcttcttt
    10561 cagaatggcc tactatcaca tatttaactc caagatcggt aagcatatca gtcgatacct
    10621 cacctgtaaa agcaccatta tcttcaaaat gtaaattttg agcagcaatc ttaatttttg
    10681 ttctttttgc tgattttaat gcagcagtta aacatacata tggaactgca caaatgacaa
    10741 tgtcttttgt ctgtttaacc ctgtctgata actctgttat aaaaatagaa gtttcagacg
    10801 gagtcttatt cattttccaa tttcctgcta ttactttttt tctactcatt tatcctccaa
    10861 tacatcaatt ccaggtaata cttttccttc taaaaattct aaagaagctc ctccacctgt
    10921 agaaatgtgt gttactttat cagcatatcc taatatttca actgctgcag cactatcacc
    10981 accaccaatt attgatacag caccagattc tgcaacagcc tttgctattt gtcttgttcc
    11041 atttgcaaag ttctcaaatt caaaaactcc cataggtcca ttccaaacta tagtttttgc
    11101 tttcaatatt tcttctgtaa atgaagctat tgtcttttca cctatatcca ttcccatcat
    11161 attgtctggt atggaacaac aatccacagt ctttttattt gcttcattac taaatgaatc
    11221 agcaacaact gaatccaaag gaagaagaag tttaacattc tttttctctg ctttttttat
    11281 taactcatcc gctaaaccga ttttatccag ttcacaaatt gatgtaccaa tttcataacc
    11341 ttttgcttta tcaaatgtgt aagccatgcc tccgcctaca agaatagtat caaccttatc
    11401 taaaaggctt tcaataacac caatcttatc agatacttta gctccgccaa gtatagcaac
    11461 aaatggtctt tcaggatctt tcaaagcttt ccccattatg tcaatttctt ttttaataag
    11521 ataaccgcac actgcaggaa gataatcagc taaacctgct gttgaagcat gagctctatg
    11581 agcagttcca aaagcgtcat taacgaatat ttcagccata gatgctaatt ctttggcaaa
    11641 agcaggatca tttttctcct cttcagcatg aaatcttaca ttttcaagca gcataacatc
    11701 accatcttta agtgaagcag ctttactttt agcatcaggc ccgatgacat cctcagctaa
    11761 tactactttt tttcctaaaa gtgaagttat tctttcggct atagggccta aggaatactt
    11821 catattaaat tctccctttg ggcgtcctaa atgagaaact agtatagttt ttgcaccatt
    11881 ttcagataaa tatctgatag ttctcattgc ttcacgtatt cttttgtcgt cagtaataac
    11941 tccatctttt aagggaacgt taaaatccat ccttgctata actctttttc cagaaacatt
    12001 gatatcttct attgtttttt tattcaatga tgccattatt attctccttt attaaaaaag
    12061 acccgcatat acgggcctta gttattattt tgcgtcaact ttagccatgt gtaaaataag
    12121 atccacgacc ttgtttgagt atccccactc attgtcatac caggcaagga gtttaacaaa
    12181 attatcattt agtgatattc ctgcttttgc gtcgaatata catgttcttg aatcaccgat
    12241 aaaatctgtt gaaacaacgt catcttcagt atatcctact atacctttaa gttcgttttc
    12301 agaagcattt ttaactgctg atttaattgc ttcataacta gcaggtttgt caagtcttac
    12361 tgtaagatca acaattgaaa catctattgt tggaactctc attgacattc ctgtaagttt
    12421 tccattaaga gaaggaatta ctttgccaac agcttttgct gctccagttg atgaaggtat
    12481 aatatttgta gctgctgctc ttccgcctct ccaatctttg tttgaaggtc cgtcaactgt
    12541 tttttgagtt gatgttgtgg aatgtacagt agtcataagt ccttcaacca caccaaaatt
    12601 atcatggact atttttacaa gtggtgcaag acaatttgtc gtacatgaag cattagaaac
    12661 aaatgtcata tcttttgtat acttgtcctg atttactccc attacaaaca tcggagtatc
    12721 atcttttgaa ggtgcggaca ttactacttt ttttgctcct gctgttaaat gtccctgtgc
    12781 cttttctttt gttaagaaaa caccagtgga ttcaacaata tattcagctc cacattcctt
    12841 ccagccaatt tctgctggat cttttgatgt gtacacttta actttctttc cgttaacaac
    12901 gagataatca tcaccagttt cgattgttcc tttaaattgt ccatgtactg aatcatactt
    12961 aagcatatat gccatataag gaacatctaa gaaaggatca ttaatacctc ttatctctac
    13021 atcagggtta tttattgctg ccctgaatac caagcgtccg attcttccga atccgttaat
    13081 tccgattttt actgtcattt atatatcctc ctaattaaaa tttaattaat taccatcatt
    13141 tattttatat taattataat acttttacaa tatcaaaatt tacttttcaa ggttaatttg
    13201 ttaaaaaatt aactatttcc atatttacca tacaattgca tggtttcaaa accaagttgt
    13261 cttagtattt catatgtaat aattgcagct gagttagata aatttaaaga tcttatattc
    13321 gaattcattg gaattcttat ggttttatta agattacttt caagtaaagt ttctggaagc
    13381 cctttagttt cttttccaaa aagaatataa acatccttag gatactttat ttccgtgtac
    13441 aaactacaag cttttgtagt tgaaaagtaa agattatcat ttttatgtat atccataaaa
    13501 ttatctaaag aatcataata aaatatatca acatcattcc aataatccaa acctgctctt
    13561 ttaagcttag aatctgtaat attaaagcct aatggtctga ctagatgaag tctagctcct
    13621 gttgcagcac aggttctggc aatatttcct gtattttgag gtatttcggg ttctactaaa
    13681 acaatattta tcatgtgatt aatataaata atcaagtatc cacatttttt gaatgtggtt
    13741 acttgattaa tttacctatt tttttattgc tcaaaatatt catttagagc tttaacaagt
    13801 gcatctgaat ttacaccatg tgccgctgaa gcttgtgcta tgctttcgcc gcttgaatga
    13861 ggacatccta tacaatgcat tccatattca aaaaatatat cagctgtatt tgggtccatt
    13921 ttcagtacat caattattat catatctttt gttactctat tcatatcagt ttctccttta
    13981 atctttctgc tatattaatt ataaaagatt ctgtgttcat tacagtgatt ttatcacttt
    14041 ctataagtct tgaaaggtct ttagtaatgt atccctgctc aattgattct aagcacgatt
    14101 tttcgagctt atttgaaaag tcaattaaat gtatatttga atccatttca cctttttttc
    14161 ttaatgcacc tgtccatgca aatattgtag caataggatt agttgaagtt tcttcaccct
    14221 taagatgttt ataataatgt cttgtaacag taccatgagc tgcttcgaac tcaaattcat
    14281 tattcggtga aactaaaacc gatgtcatca tggctaaaga accaaatgca gcagaaacca
    14341 tatcactcat tacatcacca tcatagtttt ttagagccca tatgaatccg ccttctgatc
    14401 ttactactct ggctactgca tcatctataa gcgtatagaa atattcaatt cctaaattat
    14461 cgaaattttt cttgtattca ttttcaaata tactttggaa tatatccttg aagtgatggt
    14521 catatttctt tgaaatagtg tcttttgttg aaaaccataa atccatcttt ctatcaatgg
    14581 caaagttaaa acatgatctt gcaaatgaac ttattgaaga atctttatta tgcattaatt
    14641 gtattattcc aggtccatca aaattaaatc cttctatatt tttcgttgta ccatcattat
    14701 aaacatattc tgcagatagt attcctggtc ccttaacctg atattctttg gctgaatata
    14761 catcaccata tgcatgtctt gcaataatta ttggcttttt ccagcatgat actgttggtt
    14821 taatattatt aaccaaaaca ggctctctaa atacagttcc atcaaggatt tctcttatag
    14881 tcccattagg actcttccac atttttttta atgtatattc tttcattctt tgtgcattag
    14941 gggttattgt tgcacattta acaccaactt tatgcttttt gatagcattt gcacattcaa
    15001 ctgttacaga atcatcagtt ttatctctat tatccaaaga aagatcataa tactcagtat
    15061 ttaattctat atttggtact aaaagatgat ctttgataag agtccatata actcttgtca
    15121 tttcatctcc atctatctct actataggat ttgtcattat aattttactc atttgtcacc
    15181 ttgctttcat atattgaatt tccaatgcaa ttaattataa caatagcagg aaatttttca
    15241 acatagattt ctcttaaagc ttcagttcca agatcatcgt atgctataat actttgtttt
    15301 tttatacatt ttgacagtct ggcacctaaa cctcctattg ctccaaaata aatgcattta
    15361 tttttaacta tactatcaat aacaaaattg cttcttttac ccttccctat catagcttta
    15421 agccctttgt ctaaaagata tggtgtatat ttgtccattc tgtagcttga tgtaggacct
    15481 gctgatccaa taatctgacc ttttttaggc tcggaaggac cacaatagta aatgcattga
    15541 ttttttatgt ctatgggaat tccaacttcg aacattcttt tatgtgcctg atctctggca
    15601 atatacattt ttccagttat taaaactttg tcaaaaggtt ttaatgcaca taaaatttct
    15661 tcagaaaaag gagtaaatag ttcaatcata ttactatttc cttgtgtctt aatgcatgac
    15721 attgtattgt aactgcaaca ggaagaccag ctatatgcgt aggatatttc tcaatggcta
    15781 cagatattac tgttgttttt cctccatacc cggatggacc tacattagaa ttgtttatgt
    15841 tatctttaat tttttgttcc atagcagaat aaaaagaatc tttgttattt tgatcaatag
    15901 gtctgaataa aactttctta gagagtaatg ccgccatttc catagtacct ccaatgccta
    15961 taccaacaaa aattggcggg catgcattaa ctgcattact aatgatttta tcagaaagat
    16021 aatttattat tacattaaca tcttcagatg gattgaacat tttaacaaat cccatgtttt
    16081 cactgccaaa tcctttcaac attagattta tctttaattc agagccatca actatatcag
    16141 tatatatggc agaaggagtg ttatcttgag tatttatcct gttaaaagga tcattcacaa
    16201 tcgattttct taaataacca tctgcaaatc cctttctaac tcctttatca atagaacttt
    16261 gaagtggttc ttccaaatga acattaattc caatttcagc aaaacaaata accataccag
    16321 tatcctggca taatggaatg ttttcattac tagcgatgtc tgcattataa attaattctg
    16381 aaagaacata tttcgcattt tcatttgtct caacatctat acatttttta taaattgaat
    16441 atgctttttt atcaaggttg caagcacatt caatgcatgc tctcgaaatt tcattaatta
    16501 tatcatctga ttttatcgtt cttatcatta aggtaatcct tcattgcttg tgatgactta
    16561 agtttcaatt ccattaagtc aggaactttt ataattctat ttgtagatat gtcttttagc
    16621 tgccttcctt ttctaggctt tacataaaaa gtaccaaaat ctttaatgca aactttttgt
    16681 tcctctttca gtgcttcttt tatgatgtta aagaccgtgt ttattatgtc atcactttga
    16741 agcttggtta tcttcttttc atttgctaac ctgattgata attcgttcct attcatttct
    16801 ccccctaaaa taaattattt tgaaccaaaa tactgataca aattacatag tattttccca
    16861 ttatatagtt ttcttctttt atcggcttgt ttcccatatg acaattcgaa aattttctct
    16921 ggagcaagga tatatttact ccatttacta aaagatgaaa atgcttttgg catatctgaa
    16981 tatatctgtt ttgctgtttc tatatcctct aatctttgac catatggagg attacaaatt
    17041 ataattcctt tttcatctgg tggttgtaaa tctttaaa
//
LOCUS       DAOSXM010000044        16940 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2111
            NODE_2135_length_16940_cov_9.785845, whole genome shotgun sequence.
ACCESSION   DAOSXM010000044 DAOSXM010000000
VERSION     DAOSXM010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864178
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 16940)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16940)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:24:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,261
            CDSs (total)                      :: 2,229
            Genes (coding)                    :: 2,218
            CDSs (with protein)               :: 2,218
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 3 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16940
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2135_length_16940_cov_9.785845"
                     /isolate="AMR_MDS_2111"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            190..588
                     /locus_tag="PLQ68_06785"
     CDS             190..588
                     /locus_tag="PLQ68_06785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72394.1"
                     /translation="MLKKLKLIVDPDAVLIAEYENKAVGFCLGFPDINGIIKDIDGKL
                     FPYGWAVLLKRLKNISNYRLFGLAVHPDWQNRALDALMYIQLYDNLLHKKVRMEANYI
                     LEDNYRIKNSLEKLGMMKIITYRIYEKSFK"
     gene            complement(597..1760)
                     /locus_tag="PLQ68_06790"
     CDS             complement(597..1760)
                     /locus_tag="PLQ68_06790"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HXK72395.1"
                     /translation="MTKELTTDLLKISQLAKATDTNVTTLKYYVKEGLIQPVFKTTKN
                     MAYYSHDCIQRVNMIRSLQKEHFYPLAVIKGLLDSASDPFELEFMDAIHKADLKDSNR
                     EYLLNETIKLTGLSKSQIDYLKEYRIISSEPSNNKAVYTFSDIQIMRLVKRRIDSNIP
                     FEESVKAFLIYQKALDTAAKEDVDCFIKGALLTSSPSASQAARMIGASDDTLDTFVSI
                     KRSQLNRKYGSERIRHLHHYANDLLEFLNAVSKILKINNYSDYAEKIEHSGQYFPKDN
                     DDLSQALKNYYQLINNATKSLAKSISLINQAKIYFDSFKADKIHSIEDFLIYTLKLGW
                     FSLAPNLLDYQENAKALMDNYLKISKKCGDDCIDLSKEIIEALEAIFELQTKI"
     gene            complement(1785..2993)
                     /locus_tag="PLQ68_06795"
     CDS             complement(1785..2993)
                     /locus_tag="PLQ68_06795"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HXK72396.1"
                     /translation="MKTVYFEKDIPRILATKAAAKCCKPLLYTSLNAVKYNRNIPDPP
                     LPAENWVRVKNIACGLCGTDVSFFKATTGTNSALEPIPTAKRTYLGHENVGTVIEAGS
                     KVTKFKVGDRVTIREYMSSCNNKDIKPVCKYCEEGNYNFCLNFGEPSPHNLPDTGAGW
                     SDTFIATEDSLSKVYDELTDEQAVMIEPTCVSVHSVIVDPPKKNEKILVMGCGTIGLG
                     VVQAIKIVQPECEIWVLERVKTKQQFALKLGADHIMQGEPYQAAADATNGSKVYTGMS
                     NNKMFFGGFDRIYDCIGGNWANTTALRMLRPRGILMKIGHHMRAITIDETPIWWHELK
                     LIGVDAHGMEEWEGRKLYTFDLVQEWIRDGIYKTEGFVTHHFKLDDYKQAFKLALENP
                     PDVIKIVLDC"
     gene            complement(3005..3799)
                     /locus_tag="PLQ68_06800"
     CDS             complement(3005..3799)
                     /locus_tag="PLQ68_06800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72397.1"
                     /translation="MTALSKDILTAKLFFNAAFPVMQVLLDDDPKLRKMFSNVKKTVQ
                     FGAKNENDILACHMVFNSGKVEVIQGTAENPDLALTFPSVQKMNALLTGGLALPSIKG
                     GLKNFNLLIKTLSLLMGLMKMSPNNKPKDFEGKALKVKMSLYMITRALSTYNKLGVAE
                     MVEFTQRQPDRIYQFTVEHENQKDYIACYLRIKSGKTKSGHGIYERRFPFVHFRFLSV
                     DGAIAVLGKEVEFVEGVEKGYVETIGSPEYACMLNDFMAVLQAMLT"
     gene            complement(3812..4825)
                     /locus_tag="PLQ68_06805"
     CDS             complement(3812..4825)
                     /locus_tag="PLQ68_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HXK72398.1"
                     /translation="MTIVTGTNWTAYDAATMTAREIYGRTLAQMGKENDKIVGVTADL
                     SKTTAIVHFEKAFPERFFNVGIAEQNMMGIAAGLAKAGLIPFASTMAVFASMRASEQV
                     RTDIAYQNLPVKIIATHAGISFGHAGTTHHCTEDLSIMKSMANMTVICPADGIETSKA
                     VRACIDVPGPVYIRIGRGFEPPVHENEDYNYEIGKAITMHEGSDLTIIACGIAVLQSI
                     QAAKTLKEQDDLSVRVINMHTIKPIDEQAIMDAVKDTRRILTVEEHNVIGGLGDSVAS
                     VIAKSGKGCVFKKHGIYDEYAVIGYAEDLYSHYGLDANGIIDQVRSMMGMEFEADENW
                     EDE"
     gene            complement(4827..5669)
                     /locus_tag="PLQ68_06810"
     CDS             complement(4827..5669)
                     /locus_tag="PLQ68_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012174556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HXK72399.1"
                     /translation="MPTTQEERQMLEKTACELRLKIVDVMEWSGGSHIGGSLSCADIL
                     TVLYFKYLNVDPKNPCWEDRDRFILSKGHAAAGFIPALAKKGYFPEDELRTFNHFGSP
                     FAMHPDSNKVIGCDVSSGSLGHGLSIAAGLGLGARYLKKSYKTVCLLGDGECCEGSVW
                     EAAMSISHFKLTNVITVVDRNKLMIDGKTEDVMSLEPFGEKWRAFGFEVLEIDGHNYE
                     QLTEAFDVAWAASEKPVMIIANTIKGKGIDFMENNVVYHYASGDSEVCKKAKESILRS
                     MGGK"
     gene            5894..6532
                     /locus_tag="PLQ68_06815"
     CDS             5894..6532
                     /locus_tag="PLQ68_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012199120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXK72400.1"
                     /translation="MHLFEIRSVEYLGIIKYPDLNIQKGKTTFICGKSGTGKSTLLKL
                     LNGSKSAEKGQVKYMDKLVDDYDPIEHRKQVILVNQSTYLFDMSIRDNFYEFYSYRDM
                     TKPDEDTMKQYLKLCLADFNLDMDCQNMSGGERQRIYLAICLSFMPKVIMLDEPTSAL
                     DEANAGILIRNVKNFCIEKDISLIIVSHSTELAKLYSDDCINLSGGEEQWME"
     gene            6520..7293
                     /locus_tag="PLQ68_06820"
     CDS             6520..7293
                     /locus_tag="PLQ68_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012199121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXK72401.1"
                     /translation="MDGIVQLNIFQFALVYLLLVVILVIMKNRKINQIKLLLIASVRM
                     TVQLVLAGLVLTYIFKNPHPLFTVGYIVAMIAFSIYTVLSRNKGLNKKFKIVVGASLS
                     LTGIGVILFFILAVVGVNFFNPQYTIPISGMIMGNAMTGVSLGLKTFNENVKTQRSKI
                     DTLINIGVTPNKILIPFVNSALETAILPTLNSMLGMGIISLPGMMTGQILSGTMPMTA
                     ILYQISILIAISAVVCMAVFCSLYFGYRTLYNKLSQITI"
     gene            7435..9171
                     /locus_tag="PLQ68_06825"
     CDS             7435..9171
                     /locus_tag="PLQ68_06825"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HXK72402.1"
                     /translation="MSNDHKKSKNSYKESVLFAKAKALKITVLYLLIGSLWIILSDLL
                     SQTILQDASKFMLTSILKGLLYVIVTGLAIYLMIFKALKETDMAKQDAESSNEELKKS
                     NLEYKNLYIENSNRQFLIKSLTDSMPDHIFYKDINFRYTGCNKAFAEFHGMTEKEIIG
                     KNDYQIFSKDFADTYVESDKDVINNLKKAVFEDIAHRGDSVTYFETIKNPYFDANGNC
                     VGLIGVSRDITERKVKEDQIIYLNHHDILTGLYNRLYLENAYAEIDKKENLPISIIIG
                     DINGLKLVNDSLGHGFGDMTIKEIADILSKCCSDKGVVARIGGDEFVILLPKTDNKTA
                     YDLIKQISHDCNEHMNDMKSMFYANISLGCATKSSMDDTLEKVYQQAEENMYRQKMFE
                     YKSQHSAILSSIKKTMFEKSYETEEHAERMAVLSRKLGKEVGLPEEELIALELLSNLH
                     DIGKISIDKNILTKPGKLSKEEWKEIKKHPEVGYRITQVAPDLMHISEYILSHHERWD
                     GLGYPQGLKEKEIPLLSRIIAVVDSYDAMTQDRPYSKAKTKLEAKEEILANAGSQFDP
                     IIAKIFVEKVLN"
     gene            9408..11306
                     /locus_tag="PLQ68_06830"
     CDS             9408..11306
                     /locus_tag="PLQ68_06830"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013256563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HXK72403.1"
                     /translation="MEKNYNALLQSKMSKESLEKLSALKNNKVNEFVGFFIDHCEPKS
                     VYVCTDSESDIQHVREMALSKSEEQNLANSKQTIHWDNYEDQARDKKNTRYIVYKENL
                     ENMKTLNSLEYEQGYNEILEISKGIMKGKDAVVMFLSEGPTQSVFSIPCVQFTDSWYV
                     AHSEFILYRSAYSHFLNMKEEEKDDFFRFIHSAGKLNEKGNTVNLDKRRIYMDTQNNI
                     VYSMNNQYAGNSVGMKKHSMRLAINKSRFEGWLCEHMFVMAAVDKDKDRKTYFCGAYP
                     SACGKTSTAMIPGEQIVGDDIAYFRNINGEFRAVNVEYGMFGIIKDVNEKDDPVIFEN
                     LLKDQEVIFSNVLKGPDNKPYWLGMGVDNPKTGRNHFGDWTEGVKDSKGNEVDVAHAN
                     ARYTMRLDYLDNIDKKGFESKDGVKVEGILYGGRDSDTTVPVEESPSWKDGILMKAAT
                     LESETTSATLGAEGVRTPSPMANMDFVSYPLGEYTMNNIKFGEGVKQAPKIFSNNYFM
                     RGTDGKFMTSKLAKKVWLHWADGRIHNEFEAYDTPTGKIPKYEDLAPMFKKYLNEDFS
                     KEDYTYLFTFRCTKWIDKLERTKAYYAKMDPHTPQEIFDYWDTMIEKIKTAREKFGDL
                     IKPGDYVA"
     gene            11528..14287
                     /locus_tag="PLQ68_06835"
     CDS             11528..14287
                     /locus_tag="PLQ68_06835"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HXK72404.1"
                     /translation="MIGESINNRISKPKNKLNYLLYGSSIILLSFLALVMLLSIKRTY
                     QHNLENAETMASSMSLFIPEKLFTQTKTEDASEKILNDMIFGLSTYVSSEKDIATVKI
                     IYTKDYLLQLLISTDSSDTYVDNVLYKTVYEQNTVNSSSLKLNELKNNKWLVAIPIND
                     TDTNEVIGILSAEFYTQKWYEDFIREVRWGLSISLIILIIWFGIINTFQKNIKLRLEK
                     SQLLESENKFKESETMFRTIFNQAQIGISINTVNKNIVTDTGSVFTVNKAYEKITGYT
                     KEQLRNIHWKDLTYKEDLSKEIQLMDDYLSNKIKGYVLEKRIILADGTISWTNIIISD
                     LRITNGEDRKYICILEDISNRKLMEQSLQESERSKSILLNNIPGMAFRAKLDSNRTME
                     FISEGCYDLTGYKKEYFLNKKDMSYYSIIDDKYLETVKRKWQKSILDKTRCSLEYEIR
                     TASGKYKWVFEQGQAVYNDNGEAEVIEGLVIDINTRKEHELRLRYINDHNQLTGMNNK
                     KYFQDLIDKEIEEGISGSKAVLLLNLRNFSILNVIYGYTHAESIIKELANDLQLLCEN
                     RCQIFHISVDRFILYCKGYSDKNELEQIAANIITMLDNKFITKTMGGSIGVYMLSLFE
                     SDVDLIIKKASIAADNTDRNNVFSFMFFDDVMEKQIMREAMINDELANAIYENDQNKL
                     YTVFQPIVDINTYKIIEFEALARFNSDKLGLVPPNEFIPIAEEKQMIVPMSKIIMEQA
                     FTFLKKLLDNNLDIIISINLSAIQLLREEFIGGLTDLVQRYNVPPSKICLEITETVIV
                     ENYSDINDKLFDLQQKGYSIAIDDFGTGYSSLSRESELHVNYIKIDKYFADKLLSIDM
                     HQAITSDIISMAHKLGHVVVAEGIEYECQKAYLIDNNCDLMQGYLFSKPIKESDALIL
                     AIDNK"
     gene            complement(14385..14789)
                     /locus_tag="PLQ68_06840"
     CDS             complement(14385..14789)
                     /locus_tag="PLQ68_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015006685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4332 domain-containing protein"
                     /protein_id="HXK72405.1"
                     /translation="MAKLSVIEGIGKSYEETLRAHGLKSVEKFLEACATKKGRTELAE
                     KTGISDKLILKWTNHADLNRIKGVGGQYAELLEASGVDTVVELATRNPENLFKKMTEV
                     NKEKELVRNLPTLVQVEDWVKQAAYLPRVMQH"
     gene            complement(15007..15642)
                     /locus_tag="PLQ68_06845"
     CDS             complement(15007..15642)
                     /locus_tag="PLQ68_06845"
                     /inference="COORDINATES: protein motif:HMM:NF024637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HXK72406.1"
                     /translation="MFCTNCGSKLETGQQFCTQCGTRVSSNDNIINAGNNNYNNDNTY
                     HQPAQSPQATPVWVMGASKTLSFLNIISCYVIFYNDRLLVAHITPEFQKAESAKKSAE
                     IKASNIGFFKGSAEMMRFWADYYKKYYTMSQQAILTETNLNIEITYNMVSEVKFHAFE
                     QGSDDDPDSGGYIHISVSNGQVLKLKHKISHSSSVKSTLESLFGYRLKYKR"
     gene            15979..>16940
                     /locus_tag="PLQ68_06850"
     CDS             15979..>16940
                     /locus_tag="PLQ68_06850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72407.1"
                     /translation="MNYIIEYEVCVIIFVIAIIVVFYNSHFFPSTQNKLFGSILVFTV
                     ADMILDVVTAITIEHALIMPFWINYLLNSMFYTMQIVFPVIGLFYAIAITGRLWTMCR
                     KKILLSIVPGMIVILLLIINPFTHIFFYLDKVEGYVKTPYFYFMHVVAAMYLFIVVVY
                     LIKNRKVLNKKQATTAIWFLLIIILAMCIQIIIPRLLILGPAIGISVIVMYFMLKNPS
                     NMIDYVTKAFNYNGFVTCLIEIITEKKSLNMIAIEIHDLLYIKRIFGIKSTNNLLLEI
                     SSFLRGDLKQSWVFRITDSRFIIITQKRSKHEDLKMKISQRFTKP"
BASE COUNT         5701 a         2652 c         2972 g         5615 t
ORIGIN      
        1 ggttacaagc ttcctgatag atatatgaaa ttttcagaaa atttctataa acggtatcct
       61 tacatgacat taagaagact ggatttgaag aatataaaaa aagatgctta ttcaatatgg
      121 gagatttcaa atatatcttt atctgaaaac tggtgatatg taccggttga tttatctgtt
      181 atggaggata tgctgaaaaa actgaagctt attgttgatc ctgatgctgt tttgatagct
      241 gagtatgaaa ataaagcagt tgggttctgt ctggggtttc ctgacattaa cggaattata
      301 aaagatattg acggaaagct ttttccatat gggtgggctg tacttttaaa aagacttaaa
      361 aacataagta attacaggct tttcggattg gctgtccatc ccgactggca gaacagggct
      421 ttagatgctc ttatgtatat acagctatat gataatcttt tacataaaaa agtcagaatg
      481 gaagctaatt atatacttga agataattac aggataaaaa actctttaga aaaacttggt
      541 atgatgaaaa taatcacata caggatatat gaaaaatctt ttaaatgaat atttaattaa
      601 attttagttt gtaattcaaa tattgcttct aatgcttcaa ttatctcttt tgataaatca
      661 atgcagtcat caccacattt ttttgatatc ttcagataat tatccattaa agctttggca
      721 ttttcttgat agtcgagtaa atttggtgca agactgaacc atcccaattt taaagtataa
      781 atcaaaaagt cttcaattga atgaatttta tcggctttaa aactgtcaaa atagatttta
      841 gcctgattga ttaatgatat gctttttgct aagcttttag tggcgttgtt tattagctga
      901 taataatttt tcaaagcctg tgacagatcg tcattgtctt ttgggaaata ttgtcctgaa
      961 tgctcaattt tttcagcata atctgaataa ttgtttatct taaggatttt agaaactgca
     1021 tttaggaatt ctagtaagtc atttgcgtaa tgatgaagat gccttatacg ttcagaacca
     1081 tacttcctgt ttaactgact gcgcttaatg ctgacaaaag tatcaagcgt atcatcagaa
     1141 gctccaatca ttctagcagc ttgagaagct gaaggagaag atgtcagtaa ggctcccttt
     1201 atgaaacagt caacatcttc ttttgcggct gtatctaatg ctttctgata aatcaggaag
     1261 gccttaactg attcttcaaa aggtatatta gaatcaattc ttctttttac taaacgcatt
     1321 atctgaatgt cactaaaggt atatacagct ttgttgttgc ttggttcact tgatatgatg
     1381 cgatattctt tcaagtaatc aatctgtgat ttactcagtc cagtcagttt tattgtttca
     1441 ttcaataagt actctctgtt gctgtctttt aagtctgcct tatgtatagc atccataaat
     1501 tccagttcaa agggatcaga agcactatcc aataaccctt ttatgactgc caaagggtaa
     1561 aagtgctctt tttgcaatga gcgtatcata tttacccttt gtatgcagtc atggctatag
     1621 taagccatat tttttgttgt tttaaaaaca ggctgaataa gaccttcttt gacatagtat
     1681 ttcaaagtag tcacattcgt atctgtggct tttgctagct gagatatctt cagaaggtct
     1741 gttgtcagct ctttagtcat taaattaact cctgcttttt attattaaca gtctaatact
     1801 attttaataa catcaggagg attttcaagt gcaagtttaa aagcttgttt gtaatcatcc
     1861 agtttgaaat gatgagtaac aaatccttct gtcttgtaga tgccgtctct tatccattcc
     1921 tgcaccagat caaaagtata cagttttctt ccttcccatt cttccattcc atgtgcatct
     1981 actccaatta gctttagttc atgccaccag ataggagttt catcaatagt aatggctctc
     2041 atatgatgac caattttcat caatattcct cttggtctta gcatcctaag tgcagtagtg
     2101 tttgcccagt ttcctcctat acaatcatat atgcggtcaa atccaccaaa gaacatctta
     2161 ttgttactca tgcctgtata aactttgctt ccatttgtag catcagctgc tgcctggtaa
     2221 ggttcaccct gcattatatg atcagctccc agtttcaaag cgaattgctg tttggtctta
     2281 actctttcaa gtacccatat ttcacattca ggttgtacta ttttaattgc ttgaactaca
     2341 cctagtccaa tagtcccgca tcccatcacc aggatttttt cgtttttctt gggtgggtcg
     2401 acaattacag aatgtacaga tacgcatgta ggttcaatca ttacagcctg ttcatcagtc
     2461 agttcgtcat atacttttga caacgaatct tctgttgcta taaaggtatc ggaccatcct
     2521 gcacctgtgt cgggtaaatt atgagggctt ggttctccaa agtttaagca gaaattatag
     2581 ttgccttctt cacagtattt gcacacaggc ttaatatctt tgttgttgca tgaagacata
     2641 tactctctaa tggtaactct gtctcctacc ttaaacttgg tcaccttgga acctgcttct
     2701 attacggtgc ctacattttc atgtcctaag tatgttcttt ttgcagttgg aataggttcc
     2761 agtgctgaat tcgtgcctgt ggtcgctttg aaaaaactca catcagtacc gcataacccg
     2821 catgcaatat ttttgactct cacccagttt tctgctggaa ggggaggatc tggaatgttt
     2881 ctattgtact taacagcatt taaactggtg tataacaaag gtttacagca tttagcagca
     2941 gcctttgttg ctaaaattct tggtatgtct ttttcaaaat atacagtttt cattatttcc
     3001 tccgttatgt aagcatagcc tgaagaacag ccataaagtc atttaacata catgcatatt
     3061 ctgggcttcc tattgtttct acgtatcctt tttcaactcc ttcaacaaat tcaacttctt
     3121 ttcctagtac tgcaatagct ccatccacac tcaaaaacct gaagtgtaca aaagggaatc
     3181 gtctttcata aatgccatgc ccagacttag tcttgcctga ttttattctt aaatagcagg
     3241 ctatgtagtc tttctggttc tcatgttcaa cagtaaactg atatattctg tccggctgac
     3301 gctgagtaaa ctcaaccatt tcagcgacac ctaacttgtt ataggtggaa agagctcggg
     3361 ttatcatgta aagactcatt ttaaccttta acgcttttcc ctcaaagtcc tttggtttat
     3421 tgtttggtga catcttcatc agtcccataa gtaaagaaag ggttttaata agaagattga
     3481 aatttttcaa tcctcctttg atagaaggca aagccagtcc tccggtaaga agagcattca
     3541 tcttctgaac tgaagggaat gtcaaagcta aatccggatt ttcagcagtt ccttgtatga
     3601 cttcgacttt tccactgttg aagaccatgt gacaggccag tatatcattt tcattttttg
     3661 ctccgaattg tactgtcttt ttcacattag agaacatttt tctaagtttg ggatcatcat
     3721 ccagcaaaac ctgcattaca gggaaagctg cattgaaaaa gagttttgca gtcagtatat
     3781 ccttgcttaa cgcggtcatt tcattacccc cttattcatc ttcccagttt tcatctgctt
     3841 caaattccat tcccatcatt gatctgacct ggtcgattat tccattagca tccaagccgt
     3901 agtgtgaata aagatcttca gcatagccga ttacagcata ctcatcatat ataccatgtt
     3961 ttttgaatac gcatccttta ccgcttttag ctataactga tgctactgaa tctccaagtc
     4021 ctcctattac attatgttct tctactgtca gaattcttct tgtatcttta actgcatcca
     4081 taatggcttg ttcatcaata ggcttaatgg tgtgcatatt aatgactctc acagaaagat
     4141 catcctgttc ttttaaagtc tttgcagctt gtattgattg aagaactgcg attccgcagg
     4201 cgataattgt aagatcggac ccttcgtgca ttgttatagc ttttccgatt tcataattat
     4261 agtcttcatt ttcgtgtacc ggaggttcaa accctcttcc gattcgaata tataccggtc
     4321 caggcacgtc aatacatgct cttaccgctt tagatgtttc tattccatct gcaggacata
     4381 ttacagtcat attagccata gatttcatta ttgacagatc ctctgtacag tgatgtgttg
     4441 tgcctgcatg gccaaaagat attccagcat gagtagctat gatttttacg ggcagatttt
     4501 gatatgcaat atctgttctg acctgttcag atgctctcat actagcaaat actgccattg
     4561 ttgatgcaaa gggtattaat cctgccttag ctaaacctgc agctataccc atcatattct
     4621 gctcagctat ccctacattg aaaaatcttt caggaaatgc cttttcaaaa tgaactattg
     4681 ctgttgtctt agaaagatct gcagtgacgc cgactatttt atcattttcc ttacccatct
     4741 gagccaatgt gcgaccataa atttcacggg ctgtcatagt agctgcatca taagctgtcc
     4801 agtttgttcc tgttactatt gtcatatcac ttacctccca tcgatcttaa aattgattct
     4861 ttagcttttt tgcatacttc agaatctcct gaagcataat gatagactac gttattttcc
     4921 ataaagtcta tacctttacc cttaatggta tttgcaataa tcataaccgg tttttcgctt
     4981 gcagcccaag caacatcaaa tgcttctgtc agctgttcat aattatgccc gtcaatttca
     5041 agtacttcaa aaccgaaagc tctccatttc tcaccaaaag gttctaaaga cataacatct
     5101 tctgtttttc cgtctatcat cagtttgttt cggtctacaa ctgttattac attggtaagc
     5161 ttgaaatgtg atattgacat tgcagcttcc catacagagc cttcacagca ctctccgtca
     5221 ccaagaaggc atacagtttt atatgacttt ttaagatatc ttgctcctaa tccaaggcca
     5281 gcggcaatag agagtccgtg tcctaaagag ccagaagata catcacatcc tataactttg
     5341 tttgagtcgg gatgcatagc aaaaggactt ccgaaatgat tgaaagtcct taactcgtct
     5401 tctggaaaat atcctttttt tgctaaagca ggtataaacc cagcagcagc atgtcctttt
     5461 gaaaggatga atctgtctct atcctcccag caagggtttt tcgggtccac attcaaatac
     5521 ttgaaataga ggacggtcag tatgtcagca caacttaatg agcctccaat atgtgatcct
     5581 ccagaccatt ccatgacatc aacgattttt aggcgtaatt cgcatgctgt tttttctagc
     5641 atttgccttt cttcttgtgt agttggcatt tggtatcctc cttattctgt tatgtaaaat
     5701 tataaagtag cgagtgctac tatctactat tatacatcat taaatgttaa atacaataca
     5761 ttattgctat aatttgcaaa atatgttgat attatctaca ttaatttctc tattataggc
     5821 tattgtaagg aaattactta tgatacaata atttagatta ttaattttgt cgtgttatgg
     5881 aaaagggggt cgtatgcatc tatttgaaat tcgctctgtg gaatatcttg ggattataaa
     5941 gtatcctgat ttgaatatac aaaaagggaa aacaaccttt atatgcggga aaagcggaac
     6001 agggaaaagt actttgttaa agcttctcaa cggatcaaaa tcagctgaaa aagggcaagt
     6061 gaaatatatg gataaattag ttgatgatta cgatcctatt gaacatagga aacaggtgat
     6121 attggtaaat cagtcaactt atctttttga tatgagtata agagataatt tttacgaatt
     6181 ctactcatat agagatatga caaaacctga tgaggacaca atgaagcagt atcttaaatt
     6241 gtgtcttgca gattttaacc ttgatatgga ctgtcagaac atgtcaggag gtgaaagaca
     6301 gagaatatat ctggcaatat gtctttcatt tatgccaaaa gtgattatgc ttgacgaacc
     6361 gacaagcgca ttagatgaag cgaatgcagg cattcttatt agaaatgtta agaatttctg
     6421 tattgagaaa gatatatcat taatcatcgt ttctcacagc acagaactgg caaaactgta
     6481 ttctgatgat tgtattaact taagtggagg tgaagagcaa tggatggaat agttcagctt
     6541 aatatattcc agtttgcatt agtgtatctt ttgttagtcg tgatattggt aataatgaaa
     6601 aacaggaaga taaatcagat aaaactcttg cttattgcaa gtgtaagaat gacagtacag
     6661 cttgtgcttg cagggcttgt actgacatac atatttaaaa atcctcatcc tttatttaca
     6721 gttggatata tagtagccat gattgctttt tcaatatata ctgtcctttc aagaaataaa
     6781 ggacttaata agaagttcaa gattgttgtt ggagcatcac tttcattaac aggaatcgga
     6841 gttatattgt ttttcatttt agcagttgtc ggagtcaatt ttttcaatcc ccagtatact
     6901 atacccataa gcgggatgat tatgggaaat gccatgacgg gagtatcgtt gggtttgaag
     6961 acatttaacg aaaatgtaaa aactcaaaga agcaaaatag atacattaat taatattggt
     7021 gttaccccta ataagatatt gattcctttt gttaacagtg ctctagagac ggctatacta
     7081 ccaacactga actctatgct tggtatgggt ataatatcat tgcctggtat gatgactgga
     7141 caaatattat ctggaaccat gcctatgaca gcaattctgt atcagatttc tatacttatt
     7201 gccataagcg cagtagtctg tatggcagtt ttctgttctt tgtattttgg gtatagaact
     7261 ttatacaaca aactaagcca gataactatt tgattaattg acctgatata aggacacctt
     7321 acgattttat tcatttgtga atatttttcg atgctaatat gttgtattag taattatctg
     7381 gctatataat gaaaatgtaa gtattttcaa taaatgcttt tatacggggt aaatatgagt
     7441 aatgatcata aaaagtctaa gaattcctat aaggagtcag tgttatttgc aaaagcaaag
     7501 gctttaaaga taacagtact ttatctactg ataggttctt tatggataat actatctgat
     7561 ctgttatcac agactatttt acaggatgca tcaaaattta tgctgacaag tattttaaaa
     7621 ggccttcttt atgttattgt gactgggctt gcaatatatc tgatgatttt caaagctctt
     7681 aaggaaactg atatggcaaa acaggatgct gagtcttcta atgaggaact taaaaagtca
     7741 aatcttgaat ataaaaacct gtatatagaa aatagtaaca ggcaattttt aattaagtca
     7801 ctaaccgact ctatgccgga ccatatattt tataaagata tcaatttcag atataccgga
     7861 tgtaataaag catttgcaga atttcatgga atgacagaaa aggaaataat aggaaaaaat
     7921 gattatcaga tattcagtaa ggattttgct gatacctatg tggaatcaga taaggatgtg
     7981 attaacaatc tgaaaaaagc agtatttgaa gacatagcac acagaggtga ttctgttact
     8041 tattttgaaa ctattaagaa tccatatttt gatgcaaatg gaaactgtgt tgggttaatt
     8101 ggcgtcagca gggatattac agaaagaaaa gtaaaagaag atcagattat ttacctaaac
     8161 catcatgata tactaacagg tttgtataac agactttact tggaaaatgc atatgcagaa
     8221 atagataaaa aagaaaactt acctatatcg attataattg gtgatataaa cggattaaag
     8281 cttgttaatg attcattagg tcatggtttt ggggatatga caataaaaga gattgcagat
     8341 attttaagca agtgctgctc agataaaggg gttgttgcac gaataggagg agacgaattc
     8401 gttatccttc ttccaaaaac ggataataag actgcatacg acttaattaa acaaattagt
     8461 catgactgta atgagcatat gaatgatatg aaaagtatgt tttacgcaaa tatttcatta
     8521 ggttgtgcta caaagagttc tatggatgat acactagaaa aagtatatca gcaggctgag
     8581 gaaaatatgt atcgtcagaa gatgtttgag tataaaagcc agcacagcgc aatacttagt
     8641 tccattaaaa aaacaatgtt tgaaaaaagt tatgaaactg aagaacatgc agaaagaatg
     8701 gctgttcttt caagaaaact tggtaaagaa gtgggtcttc cagaagaaga acttatcgct
     8761 ttggaattat tatctaattt gcatgatatt ggaaaaatca gtatagataa aaatattctt
     8821 actaaaccag gaaaactcag taaagaagag tggaaagaga ttaaaaagca ccctgaagta
     8881 ggttatagga taacacaagt agctcccgat ttaatgcaca tatcagaata tattctgagc
     8941 catcacgaaa gatgggatgg attaggatat cctcagggac tgaaagaaaa agaaataccg
     9001 cttttgtcaa gaattattgc tgtagtagat tcatatgatg caatgaccca agacaggcca
     9061 tatagtaaag caaaaacgaa actagaagca aaagaagaaa tattggcaaa tgccggcagt
     9121 cagtttgatc cgataattgc aaagatattt gttgaaaaag ttttaaatta atcaattttc
     9181 ataaaatcat ataattatag aaataaaacc gttttatctg ttgtattaaa caaattgttc
     9241 ttattaagtt cacaatttta aggtatgctt ttattgacat gtgattatgt aaaaggtaaa
     9301 attagtaatg tgttatatga actaattaaa atgggcgaga aggaccataa aaacacaaaa
     9361 aatacatatc tgtaacataa agatgtgtaa aatataaagg agaagacatg gaaaaaaatt
     9421 ataatgcact attgcaatcc aagatgagca aagaaagttt agagaagctt tcagcattaa
     9481 aaaacaacaa agtaaatgaa ttcgtaggat tctttattga tcattgcgaa cctaaatccg
     9541 tatatgtctg tactgatagc gaaagcgata tccagcatgt acgtgaaatg gctttatcta
     9601 agtcagaaga acagaatctt gcaaattcaa aacaaactat ccactgggat aattacgagg
     9661 atcaggcaag agataaaaag aatacacgtt atattgtata taaagagaat cttgaaaaca
     9721 tgaagactct taactcttta gagtatgagc agggatataa tgagattctt gaaatatcaa
     9781 aaggtataat gaagggcaaa gatgcagtag taatgttttt aagtgaagga cctactcaga
     9841 gtgtattctc aataccatgt gttcaattta cagatagctg gtatgtagct cattcagagt
     9901 ttattcttta tcgttcagca tattcgcatt tcttgaacat gaaagaagaa gaaaaagacg
     9961 atttcttccg tttcatacat tcagcaggca agttaaatga aaaaggaaat actgttaatc
    10021 ttgataaacg tagaatatat atggatactc agaacaatat tgtttattct atgaataatc
    10081 agtatgcagg aaactcagta ggtatgaaaa agcattcaat gagacttgca attaacaagt
    10141 ctagatttga aggatggctt tgtgagcata tgtttgttat ggctgctgtt gataaggata
    10201 aagacagaaa gacatatttc tgtggcgcgt atccgtcagc atgcggaaaa acttccacag
    10261 ctatgatacc tggagagcag atagttggtg acgacatagc atatttcaga aatattaatg
    10321 gtgagttcag agctgttaat gttgaatacg gtatgttcgg tattataaaa gatgttaatg
    10381 agaaagatga tcctgtaata tttgaaaacc ttttaaaaga ccaagaagta atattctcaa
    10441 acgtacttaa aggtcctgac aataaacctt actggttagg tatgggagtg gataatccta
    10501 agactggaag aaaccatttt ggtgactgga cagaaggcgt taaagacagt aaaggtaatg
    10561 aagttgatgt agctcatgca aatgcaagat atacaatgag acttgattat cttgataata
    10621 ttgacaaaaa gggatttgaa tcaaaagatg gcgttaaagt agaaggtatt ctatacggag
    10681 gtcgtgattc agatactaca gttcctgttg aggaatcacc ttcatggaaa gatggtatat
    10741 tgatgaaggc tgctacatta gagtctgaaa caacaagtgc tactttggga gcagaaggtg
    10801 tgagaacacc atcacctatg gctaatatgg actttgtgtc ttatccatta ggagaataca
    10861 caatgaataa catcaaattt ggagaaggtg taaagcaggc tccgaaaata ttcagtaata
    10921 actacttcat gagaggaaca gatggaaaat tcatgacaag caagttagct aagaaagttt
    10981 ggctgcactg ggctgatggc agaattcaca atgaatttga agcatatgac acaccaacag
    11041 gaaaaattcc gaagtatgaa gatcttgctc caatgtttaa aaaatattta aatgaagatt
    11101 tttcaaagga agattacaca tatttgttta ccttccgttg cacaaaatgg attgataaac
    11161 ttgagagaac taaagcatat tatgcaaaga tggatccgca tactcctcag gaaatattcg
    11221 attactggga tacaatgatt gagaagatta aaacagccag agaaaaattt ggtgatttaa
    11281 tcaaaccagg agattatgta gcttaaaaca taaattaaaa ttaaaagaac atggctgaaa
    11341 atgctgtgtt cttttttttg cagtagattt taaaatattg taaatacgtt agtgtaaaga
    11401 gttttggggt aaatacaaaa agattatcaa tatggcataa ataagatata ataataattg
    11461 gaatgattgt agattttttg aattatatat ttatacttat cgaaaaatta tagtctaggg
    11521 tgctaatatg ataggggaat ctattaataa cagaatttca aaaccaaaaa ataagttaaa
    11581 ttacttattg tatggttctt cgattatatt attatccttt ttagcattag tcatgctact
    11641 atcaataaaa agaacatatc aacataatct tgagaatgca gaaacaatgg caagttcaat
    11701 gtcacttttt atacctgaaa agttatttac gcaaactaag acagaagatg catctgaaaa
    11761 aatattaaat gatatgattt ttggactatc gacctatgtc tcatcagaaa aggatatagc
    11821 aactgttaaa ataatttata ctaaagacta tttattgcaa ttactgataa gtactgattc
    11881 atctgataca tatgttgaca atgttcttta caaaacagtt tatgagcaga acacggttaa
    11941 ttcatcttcg ttaaaattga atgagctgaa gaataataaa tggcttgtag caataccaat
    12001 aaacgatacc gatacaaatg aagttatagg gattctgtct gcagaatttt atacacaaaa
    12061 gtggtatgag gattttatca gggaagtgag atggggtctg tcaatatctt taatcatact
    12121 tattatctgg tttggcatta ttaatacatt tcagaaaaat attaaactta gactagaaaa
    12181 atcacaactt cttgaatcag aaaacaaatt taaagaaagt gaaacaatgt ttcgaactat
    12241 atttaatcag gctcagatag gaatatctat aaatactgtg aataaaaata tagtaacaga
    12301 tacgggttca gtttttacag taaacaaagc atatgaaaag ataacaggat atacaaaaga
    12361 gcaattgaga aatattcact ggaaggatct gacttataaa gaggatttat caaaagaaat
    12421 tcagctgatg gatgattatc tttcaaataa gattaaagga tatgtgcttg aaaaaagaat
    12481 aatactggct gatgggacta tatcctggac aaatatcatt atatccgatt tacgtatcac
    12541 aaatggtgaa gatagaaaat atatatgtat tcttgaggat atttctaata gaaagttaat
    12601 ggaacaatca ttacaagaaa gtgaaagaag caagtcaatt ttattgaaca acataccggg
    12661 tatggccttt agagcgaaac ttgatagtaa caggaccatg gagtttatat ctgaaggctg
    12721 ttatgacctt acaggctaca agaaggaata ttttttgaat aagaaggaca tgtcatacta
    12781 ttcaataatt gatgataagt atttagagac agtaaagaga aagtggcaga aatcgatttt
    12841 agataaaaca aggtgctcac tggaatacga aataaggaca gcatcaggca aatataagtg
    12901 ggtttttgaa caaggacaag cagtatacaa cgataatggc gaagcagagg tcattgaagg
    12961 cttagttata gatataaata caagaaaaga acatgagctc aggctgagat atataaacga
    13021 ccataatcaa ctgactggaa tgaataacaa aaaatatttc caggatctga ttgataaaga
    13081 aatagaagag ggaatttcag gttcaaaagc agttctttta ttaaatctca ggaatttcag
    13141 cattttaaat gttatatacg gatatacaca tgctgagagt attataaaag agcttgctaa
    13201 tgatttgcaa ttattatgtg aaaacagatg tcagattttt catatatctg ttgatagatt
    13261 catattgtat tgcaagggtt actctgataa aaatgagctt gaacagattg ctgcaaatat
    13321 catcactatg cttgataata aatttattac aaagaccatg ggtggaagta tcggggtata
    13381 tatgcttagc ttgtttgagt ctgatgtaga tttgataatt aaaaaagcat caattgctgc
    13441 agataataca gatagaaaca atgtattctc atttatgttt tttgatgatg taatggaaaa
    13501 acagattatg agagaggcaa tgattaacga tgaacttgct aatgctattt atgaaaatga
    13561 ccaaaacaaa ctgtatacag tttttcagcc gattgttgat attaatacat ataaaattat
    13621 tgagtttgaa gcacttgcaa ggtttaatag tgataagtta ggattagtgc ctccaaatga
    13681 attcatacct atagcggaag aaaaacagat gattgtacct atgagtaaga ttataatgga
    13741 acaagcattt actttcttaa aaaaattatt agataataat ctcgatatta ttatttcaat
    13801 taacttatcc gcaatacaat tgctgcgaga agaatttatc ggcggtctga cagatttagt
    13861 tcaaagatat aatgtacctc catcaaaaat atgtttagaa attactgaaa cggtaatagt
    13921 cgaaaattat tcagatataa acgacaaact ttttgatcta cagcaaaagg ggtactcgat
    13981 agcaatagat gattttggta ctggatactc atctttatca agagaaagcg aattacatgt
    14041 aaattatatt aaaattgata aatattttgc ggataaacta cttagtattg acatgcatca
    14101 agcgataact tcagatataa tttctatggc tcacaaactt ggacatgttg ttgtagcaga
    14161 gggaatagag tatgagtgtc aaaaagcata tctgattgat aacaattgcg accttatgca
    14221 ggggtattta ttctcaaaac cgataaaaga gtcagatgct ttgattttag ctattgataa
    14281 taaatagaaa caacttttat gtacaaagca aaacacctct gttacctata ccgtaacata
    14341 tgaggtgttt tgcatgaaaa tcaattttta attgatttga cagattaatg ttgcattacg
    14401 cgtggaagat atgcagcttg ttttacccaa tcttcaactt gaactaatgt tggaagattt
    14461 ctaacaagtt ccttttcctt attaacttca gtcattttct tgaaaagatt ttctggatta
    14521 cgggtcgcaa gctcaactac tgtgtctaca cctgaagctt caagtaattc agcatactgt
    14581 ccgcctactc ctttaattct gtttagatca gcatgatttg tccatttaag tatcagttta
    14641 tctgaaatac ctgttttttc agcaagttct gttctgcctt tttttgtagc acaagcttct
    14701 aagaattttt ctacagattt caaaccatgt gctctaagtg tttcttcata acttttacca
    14761 atgccttcta taacgcttaa ttttgccatt ttaattaatt cctcctttaa tttataaaat
    14821 aattatatat gtcgcgtatg tttggctaca gtgatattgt cacacatttt tcacaattaa
    14881 tcaagactgt atattaataa agacgaaata ttatataata tatagaatta ttaaaaaata
    14941 cactaaataa attagcataa ttgtgctttg attatgatta tttgaaattt attgaaaatt
    15001 tatgtattag cgtttgtatt tcagcctata tccgaaaagt gattccaatg tactcttaac
    15061 agaactggaa tgactgattt tgtgcttcag tttcagaact tgtccattac taacagatat
    15121 gtgtatataa ccgccagaat ctgggtcatc atcagaacct tgttcaaatg catgaaactt
    15181 tacttctgat accatattgt atgtaatttc aatattcaaa tttgtctcag taagaattgc
    15241 ctgctggctc atggtgtagt attttttata gtaatctgcc cagaatctca tcatttcagc
    15301 actaccctta aaaaaaccta tattacttgc ttttatttct gctgattttt tagcgctttc
    15361 tgctttttgg aattcaggag taatgtgcgc aactaataac ctgtcattgt aaaagataac
    15421 atagcagcta ataatattca ggaatgataa ggtttttgat gctcccatta cccacactgg
    15481 tgtagcttgt ggtgattgag caggctgatg atatgtatta tcattattat aattgttatt
    15541 tcctgcatta ataatattat cattactact tactcttgta ccgcattgag tacaaaactg
    15601 ctggccagtt tccagcttgc ttccgcaatt tgtgcaaaac ataatatacc tccaaagaca
    15661 aataaattat atcccaatct atatttgttt acaatatagt taagatttat ttgaatcgag
    15721 atatattgat gtataaaaat gtaataagga aatatgagta atcgctttaa ctaaaaataa
    15781 ttattttgtg gacttaaatg attttcttgc ttataataaa ttcatgaagt gaaagaataa
    15841 tcttcaaatg atattatttt attcattaaa gaatggtaaa attcatctta agtgttttat
    15901 tattcagaat attgattttg atgataaaca agaaaattcg ttttttaagt atcgatttgc
    15961 taatcaaggg agaaatttat gaattacatt atagaatacg aagtttgcgt aataatattt
    16021 gtaatagcaa ttattgttgt attctacaat agtcatttct tcccaagtac acaaaataag
    16081 cttttcggtt cgattttagt tttcacagtt gcagacatga tacttgatgt agttactgca
    16141 ataaccatag aacacgctct gattatgcca ttttggatta attatttatt aaattccatg
    16201 ttttatacca tgcagattgt ttttccggtt attggacttt tttatgcaat agctattact
    16261 ggtcgattat ggaccatgtg ccgaaaaaaa atactgttat ctatagttcc aggtatgatt
    16321 gtaatattgc tgttaataat taatcctttt acccatatat ttttctacct tgataaagta
    16381 gaaggttatg tcaagactcc ttatttctat ttcatgcatg ttgtagctgc catgtattta
    16441 tttattgtag tagtgtatct tattaaaaat cgaaaagttc ttaataagaa gcaagctacc
    16501 actgcaattt ggtttttatt gattataatt ttagcaatgt gtatacagat tattattcct
    16561 cgacttttaa ttttaggacc agcaataggt atatcagtta tagtaatgta tttcatgctg
    16621 aaaaatccaa gtaatatgat tgattatgtc acaaaagcat ttaactataa cggatttgtg
    16681 acttgtttga ttgaaataat aactgaaaag aagagcctca atatgatagc aattgaaatt
    16741 catgatctgc tatatattaa aagaattttc ggtataaaaa gtacgaataa tcttttattg
    16801 gaaatatctt cttttttaag aggtgactta aagcaaagct gggtatttcg aattactgac
    16861 agccggttta taataatcac tcagaaaaga agcaagcatg aagatttgaa aatgaaaatt
    16921 tctcaaagat ttacaaaacc
//
LOCUS       DAOSXM010000045        16744 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2111
            NODE_2161_length_16744_cov_10.484511, whole genome shotgun
            sequence.
ACCESSION   DAOSXM010000045 DAOSXM010000000
VERSION     DAOSXM010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864178
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 16744)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16744)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:24:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,261
            CDSs (total)                      :: 2,229
            Genes (coding)                    :: 2,218
            CDSs (with protein)               :: 2,218
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 3 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16744
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2161_length_16744_cov_10.484511"
                     /isolate="AMR_MDS_2111"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2343
                     /locus_tag="PLQ68_06855"
     CDS             <1..2343
                     /locus_tag="PLQ68_06855"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HXK72408.1"
                     /translation="SQSQIYKAFAITVKNEIMQKWAASERKPDKKQVYYLSIEFLMGR
                     ALSNNILNIGRYDEYLQACKELGLDFMSIVSSEPDAGLGNGGLGRLAACFIDSLATLD
                     LPAQGCGIRYEYGLFKQKIIDGYQVEYPDAWLEDGNVWEVERPEEKMEVHFGGQILES
                     WEGDKLRFVHSDYTTVLAVPYDMPIIGYMNDRVNSLRLWKAVSPKHLDMVSFNKGEYL
                     KAVEEREMAEVLSHILYPEDRHYEGKSLRLKQHYFFVSATCQMIVSKFLKTNQPIEKL
                     PQTAVIHINDTHPALAIPELMRILMDEQGLGWEQAWEITSRMFAYTNHTIMAEALEKW
                     PENLFMQVLPRIYSIVKEINERFCSMIYKDFPEKRNDIHKMAIIAYGQIAMAPLCIAG
                     AYSINGVSQLHSNIIKEDTFKDYADIFNDKFTNVTNGVTHRRWLMLSNKNLSDLITSK
                     IGKEWISDTLKLKQLEEFADNKTFSDKFDKIKRNNKKKLAEYIRNTTGIIVSEDSIFD
                     VQAKRLHEYKRQLMNIIRILYNYIEIIEDPDKYREARPITCIFAAKAAPGYNRAKTII
                     KLINDVANLVNNDTRIMDKIKVVFLENYSVSLAQMIFPASDISEQISTASKEASGTGN
                     MKFMLNGALTLGTLDGANVEIHESVGDENIYIFGFDSKTVQQIYETGLYDPRIYLNED
                     PKLKKALDMLIDGSINSERLNLYKEIYDSLLSCRSSMSDQYLIMGDFKSYKETHDKMV
                     ADYEDRNSWNKKAIINVANAGRFSSDISIANYNKRIWKLK"
     gene            2343..4082
                     /locus_tag="PLQ68_06860"
     CDS             2343..4082
                     /locus_tag="PLQ68_06860"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HXK72409.1"
                     /translation="MLEIFYNSRDTVYKSIFGAVQCATLIKFRIDVRCDAPVKAAIII
                     NHISHEMQIDSLTGDLSVFTLSLHSLHKPGLMYYHFEVSTPYHTVYYGNDMDMLQGIG
                     KKYDKKPLDYQITVYDKFNTPDWIKGRVMYQIFPDRFYKENIKQSPYWDRYFHESWYE
                     DPYIEDDFKSGTMECSDFFGGNLDGIIKKLDYIKSLGVGVIYLNPIYLAYSNHRYDVA
                     DYMSVDDTLGEENDLRILCEEAKKRDIYIILDMVFSHTGSDSVYFNKNMTFGYQGAFN
                     DKQSEYRSWYVFKKDNTYESWWGIETLPNTIETNPSFMSHTLSSLEKWMNLGIKGFRL
                     DVADELPDEYIKALRSKIKSIDNQSVLIGEVWEDASNKSSYGKKREYLYGYELDSVMN
                     YPYRDALINYTLGKIDAKTFCRKIEIIRENYPKQVYECLMNMLSTHDVSRILSVLGGK
                     SDFENIPRNQQKNIYLNKEEYEIAVKRLNFIISLLICLPGVPTIYYADEAGSQGLKDP
                     FNRRTYPWGKEDLRIFEIYKGFLNQRSSNELLRYGTFSISEKEDNILIKREYGQEVST
                     FIFNNTKLSYIKV"
     gene            4154..5482
                     /locus_tag="PLQ68_06865"
     CDS             4154..5482
                     /locus_tag="PLQ68_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015926766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HXK72410.1"
                     /translation="MSKITFMGAGSSVFVKNVIGDCMCTPVLADSEFALYDIDEQRLN
                     DSKLLVDTLNKNINNNKATIKSYLGVENRKDALRDADFIVNAIQVGGYEPCTVIDFEI
                     PKKYGINQTIADTLGIGGIMRALRTIPVMKEFATDIEQVCPNALLLNYTNPMSMLAGY
                     MQRYTKVKTVGLCHSVQVCSEAVLNGVGMQDKLEGRVDFIAGINHMGWLLDIKDKDGN
                     DLYPEIRKKAAIKNKTEKHKDMVRFEYIKHLGYYCTESSEHNAEYNPWFIKPNYPELI
                     EKFNIPLDEYPRRCRNQISRWAEQKQELLNNGRIDHERSREYASYIMEAVVTNKPFKI
                     GGNVMNTGLIENLPADACVEVPCMIDGNGINPCHVGRLPVQLAAMNMTNINVHLLTIQ
                     AAVTKKREDIYRAVMLEPRCAAILSVDDIIKMVDELIEAHQRVGFMTEYK"
     gene            5555..5905
                     /locus_tag="PLQ68_06870"
     CDS             5555..5905
                     /locus_tag="PLQ68_06870"
                     /inference="COORDINATES: protein motif:HMM:NF016187.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OadG family protein"
                     /protein_id="HXK72411.1"
                     /translation="MENALLQTITGFLVVIIGMLILTAVIILFSKLMGKNSTNKTVAV
                     KQVEKPVTPVAEIYADFDDEEEIAAVMACIEAYLQQNSIKNVCNIVVRPLVRTVSSST
                     WSQAGRINLVNKKV"
     gene            5919..7085
                     /locus_tag="PLQ68_06875"
     CDS             5919..7085
                     /locus_tag="PLQ68_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014116975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HXK72412.1"
                     /translation="MLLESLKDLFNQSAFVNITWQQGILIIVSLVLLYLGIVKKFEPL
                     LLVPIAFGMLATNLPGSNVFNPELWENMKNINYGDVLRNGGLLDILYIGIKVGLFPPL
                     IFMGIGAMTDFGPLIANPKSFILGASAQFGIFSAYFLATKLGFTAAESGAIGIIGGAD
                     GPTAIYTASILAPHLLGAIAVAAYSYMALVPVIQPPFMKMLTTKKERAIVMEQLRPVS
                     KTEKIIFPILVTIVVAFLIPSAAVLVASLMVGNLLKESGVTDRLSKTLQNEFINIITI
                     CLGLAVGATATASNFLKAETLKILGLGVLAFIFGTITGILFAKLMNVFSKKKINPLIG
                     SAGVSAVPMAARISQKVAQSENPNIFLLMHAMGPNVAGVIGSAIAAGVLISLLG"
     gene            7281..8630
                     /gene="gdhA"
                     /locus_tag="PLQ68_06880"
     CDS             7281..8630
                     /gene="gdhA"
                     /locus_tag="PLQ68_06880"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018998675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HXK72413.1"
                     /translation="MKFKSPYIARVYEDIVARNPNEPEFHQAVKEVFETITPVIENND
                     IYEKHGILERITEPERLITFRVTWVSDDGKVNVNRGYRVQFNSAIGPYKGGLRLHPSV
                     NASVLKFLGFEQIFKNSLTGLPMGGGKGGSDFNPKGKSDMEIMRFCQAFMSELYRHIG
                     PNLDVPAGDIGTGAREIGYLFGQYKRLANEFTGVLTGKGLAYGGSLVRKEATGYGLCY
                     FTEEMLKGLKNDSIKNKKVCVSGSGNVAIYAIEKIYELGGLPVTASDSDGFIYDPNGI
                     DLDLLKKLKEVERKRIFEYADIVKGAEYFEGQRPWAIPCDIALPCATQNELELDDAKK
                     LVENNVIAVAEGANMPCTPEAIEYFQNSKVLYGPAKAANAGGVATSGLEMSQNSMRYS
                     WTFEKVDERLKAIMKGIFDNVSETAKTYGVEGDLMAGANMAGFLKVADAMIAQGIIF"
     gene            9020..10036
                     /gene="pheS"
                     /locus_tag="PLQ68_06885"
     CDS             9020..10036
                     /gene="pheS"
                     /locus_tag="PLQ68_06885"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HXK72414.1"
                     /translation="MLQLDNMLITAQSEISSAKDMKRIEELKVQYLGKKGELTQILRG
                     MSSLSPEERPAAGKAANEAREKLNMLFDEMEQKIQYNVLNERLEKETIDVTMPGVKNE
                     IGSMHPLSQVIQKIEDIFIGLGYQIAEGPEIETVHYNFDQLNTPENHPSKDEQDTFYF
                     DAQKLLRTQTSPVQARVMERTNPPIRIICPGRVYRSDEIDATHSPLFHQVEGLVVDKG
                     ITMGNLVATLKLFAKELFGEFTEIRLRPHYFPFTEPSAEVDVSCWVCKGKGCPVCKYE
                     GFIEILGAGMVHPNVLRNCGIDPDIYTGFAFGLGVERTAMAVFGINDLRLLYENDMRF
                     LSQF"
     gene            10047..12425
                     /gene="pheT"
                     /locus_tag="PLQ68_06890"
     CDS             10047..12425
                     /gene="pheT"
                     /locus_tag="PLQ68_06890"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HXK72415.1"
                     /translation="MKTPISWLRDFVKIDRDIKSFCDLMTMSGTKVEGYSIVGKEINR
                     VVTGKILELWNHPDSDHLLIAKVDVKESVLQIVTGAPNVKVGQIVPIALDNSTLPKGK
                     IIRKGKLRGELSEGMMCSVQELNLTKHDYPDACDDGIWILKEDTPIGMDIKKYAGLDD
                     TVVEFEITPNRQDCYCIKGIAREASAALNVPIKEKQIALKEQGKGKSSDYIKITISAY
                     DLCSRYCGRIITDVVIEPSPEWMRQRLRNAGVRPINNIVDITNYVMLELGQPMHAFDL
                     NYIEGGLINVRRAYNDELVITLDEKERELDSSMLVISDAKKTVAVAGIMGAMNSEVNE
                     NTKTVLFESAMFNPTSIRITAKKLGMRTDSSALFEKGLDANNTLTAINRACELVEMIG
                     AGKVSKGIIDVHKELPMPNSVLLDSDKINTLLGTDIKINDMKSILTRLGMSIKSDNTV
                     VVPTFRPDVSTYADLAEEIARLYGYNKIVPSLTLGQSVTQGARTHIQNVRKTISETLV
                     CCGLYEIMTYSFISPKNFDKLGLDMNNPLRDCMVIKNPLGEDFSVMRTTTIPGMLKVL
                     SYNSNRNLQKARFFEIGKTYHPSDNLAKEIDYITVGMYGSCDFYDIKGVAESVLKRLG
                     ITDVFYSTNMENEVFHKGRCADIIHDGSKIGILGQIDYKVTEAFDAPDESYILIMPIE
                     KLAQKTDFSRNFKPLPKYPSSQRDLSVVVKKSILSADLLNESKKVARDILEDVQLFDM
                     YTGGQIEEGYKSLSYAFTFRSIERTLEEDEVNKAMSDIINILEKKFDAKIRC"
     gene            12419..12811
                     /locus_tag="PLQ68_06895"
     CDS             12419..12811
                     /locus_tag="PLQ68_06895"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HXK72416.1"
                     /translation="MLEDVYKRFENDRFAYGLLNCEIVEISEGKASASLMVKRDHLNG
                     VDLCQGGVIYSLADFSCAMAANSYGPVCVTLENNIIYKRSVKEGEIMIASSFVIERMK
                     KILICECVITVNHEIRAIMKSKLLSVEQ"
     gene            12853..13977
                     /locus_tag="PLQ68_06900"
     CDS             12853..13977
                     /locus_tag="PLQ68_06900"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HXK72417.1"
                     /translation="MFNKISVRTFFKKFDYILLGLVLALTAIGIVAMPSVVDTMNSGP
                     SILKTQLFSIALGLFLCLGFSILDYSFLRYWGIAFYIIGLLMLMYVIPFGYGRDNVNI
                     GSNSWIDLFGVFSFQPSELMKVAYMMFLPSQFELLKEEFSLKRLIFIAISGILPIGLI
                     LMQPDLGTATVFAFSFAVLIFVYGIKYRYIILSLLALGISLPFIWMFLDDWQKDRIRV
                     FIDPTFDPTGAGYQTSKSIVALGSGGWSGAGLFNGIQTQGNGIPIKESDFIFSAIGEE
                     LGFIGCAAIVLLAFGIIIRCIYIASKCASYYGQFIVAGMSAMIAFHFIENIGMNIGLL
                     PVTGIPLPFISAGGTNLIGSFAMIGIIISASIRREQVKRL"
     gene            14150..14623
                     /locus_tag="PLQ68_06905"
     CDS             14150..14623
                     /locus_tag="PLQ68_06905"
                     /inference="COORDINATES: protein motif:HMM:NF014439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HXK72418.1"
                     /translation="MAMYGEFTHRVDDKGRVILPVSMREVLGDKFVIAKSSDSTLKIL
                     YLYTREDWEKIIEAGARIAAKDRSKATYVRQLSASGKDTELDSQNRVLIPIELRNYAN
                     IGKETVLLGLGNRVEIWNKESYYINHPDETEVTKEKAQEDADKVASLLEEIALLQ"
     gene            14620..15564
                     /gene="rsmH"
                     /locus_tag="PLQ68_06910"
     CDS             14620..15564
                     /gene="rsmH"
                     /locus_tag="PLQ68_06910"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003475471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HXK72419.1"
                     /translation="MKEYSHESVMADECIEYLNIDKRGTYIDCTAGGGGHSVRIYERL
                     NEDGLLIACDQDIKACEVTKERLEKYNRKTKFIVLHENFVNIDKVISEYCPEGADGIL
                     LDLGVSSYQLDNEERGFSYHMDGPLDMRMDQTKDKTAYDVVNNYTKEELSQILFEYGE
                     EKHSRRIAEAIVHKRKDELISTTNQLAEIIKEAYPAKERFKGKHPARKTFQAIRIEVN
                     NELTVLKQMLPKAAFSLKSKGRLAVITFHSLEDRIVKNAFKELIDPCTCPKSFPVCVC
                     NKVAQHKLIVKKPLVPKSEETQKNYRSRSSKLRVLERT"
     gene            15693..16112
                     /locus_tag="PLQ68_06915"
     CDS             15693..16112
                     /locus_tag="PLQ68_06915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72420.1"
                     /translation="MEIERRNENNIIGKLKAVILLMLVFTALFLIIFRYGEMINLNYD
                     ITRLNNELREKTGINSALYAELDKQVTINDIKYTAETKLDMNKPRNDQIIYINTKLSD
                     DYNSQEKDNFYTMTSSVYERINHTFKNIVEIFKGSNE"
     gene            16149..>16744
                     /locus_tag="PLQ68_06920"
     CDS             16149..>16744
                     /locus_tag="PLQ68_06920"
                     /inference="COORDINATES: protein motif:HMM:NF015662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72421.1"
                     /translation="MNDIENKRSNKKENVKTEVVYNNNLTRKRLLVLMAVFIIIAMVI
                     AGYTGYIQLVWATELKVKAYEQQNQDRTIKASRGTVYDCEGNVLAISISVDTISISPV
                     TVQKNKDITVEEIASDLSNILNLEYESTLNKLKSESQYQLIASKVDRQIGDVIRDYIK
                     TNSVAGIYVQQDSKRYYPYGSLASHAIGFAGDDNQGLFG"
BASE COUNT         6002 a         2249 c         3413 g         5080 t
ORIGIN      
        1 agccaaagtc agatttataa ggcatttgca ataacggtta aaaatgagat aatgcagaag
       61 tgggctgctt cagaaagaaa acctgataaa aaacaggttt attatctttc catagagttt
      121 ttaatgggta gagcattatc taataatata ttgaacatcg gtagatatga tgaatatcta
      181 caggcatgta aagaactggg attggatttt atgtcaattg tatcatctga acctgatgca
      241 ggacttggca atgggggatt aggacgttta gcggcctgtt ttatcgactc tttagcaaca
      301 ctggatcttc ctgcacaagg atgcggtata agatatgagt atggattgtt taagcagaag
      361 ataattgacg gttatcaggt tgagtatcct gatgcatggc ttgaagatgg aaatgtctgg
      421 gaggttgaaa gaccagagga aaaaatggaa gtccattttg gaggacagat attagaatca
      481 tgggaaggtg acaaattgag atttgtccat agtgattata ctacagtact agccgttcca
      541 tatgatatgc ctataattgg atatatgaat gacagggtta attctttaag gctctggaaa
      601 gcagtatctc caaagcacct agatatggtc agcttcaata aaggagagta tcttaaggca
      661 gtagaagaaa gagaaatggc agaagtgctt tcacatatct tatatccaga agacagacat
      721 tatgaaggga aatctttaag actgaaacag cactatttct ttgtttctgc aacctgtcag
      781 atgatagtat ctaaattttt aaaaaccaat caacctattg aaaaactgcc tcaaaccgca
      841 gtaattcata ttaatgacac acatcctgcg ttagcaatcc ctgagcttat gaggatatta
      901 atggatgaac agggattagg atgggagcaa gcttgggaaa taacctcaag aatgtttgca
      961 tatactaatc acacgataat ggctgaagct ttagaaaaat ggccagagaa tcttttcatg
     1021 caggtactgc caagaatata cagtatagtt aaagagatta atgagcgttt ctgttcaatg
     1081 atttataagg attttcctga gaaaagaaat gatattcaca aaatggcaat aattgcatac
     1141 ggtcaaatag ctatggctcc attatgtata gcaggggcat attcgataaa cggagtatca
     1201 caacttcatt caaatataat aaaagaagat acattcaaag attatgctga tatatttaat
     1261 gataagttta caaatgtaac taatggagtt acacatagaa gatggcttat gctatctaac
     1321 aaaaatttat ctgatctgat tacatctaaa ataggtaaag agtggattag tgacacacta
     1381 aagcttaaac agttagaaga gtttgctgat aacaagactt tttcagataa gtttgataag
     1441 ataaaaagaa ataacaaaaa gaagcttgct gaatacataa gaaacactac aggtataata
     1501 gtaagtgaag attccatatt tgatgttcag gcaaaaaggc tccatgaata taaaaggcag
     1561 ttgatgaata tcataaggat actatacaac tatatagaaa taattgaaga tcctgataaa
     1621 tacagagaag ccagacctat aacatgtata tttgctgcga aagcagcgcc aggatataac
     1681 agagcgaaaa ctataattaa gctaattaat gatgtagcaa atcttgttaa taatgatact
     1741 aggataatgg ataagattaa ggttgtcttt cttgagaatt attcagtttc attagcacag
     1801 atgatatttc ctgcaagtga catatcagaa cagatttcaa ctgcctccaa agaagcgagc
     1861 gggactggaa acatgaaatt tatgcttaat ggtgcattaa cacttggtac tctggatggt
     1921 gcaaatgtag aaatacatga aagtgtcggt gatgaaaaca tatacatttt cggatttgat
     1981 tcaaaaactg tacagcagat ttacgaaaca ggattgtatg atcctagaat atatttaaat
     2041 gaggatccga aacttaaaaa agcattggat atgcttatag acggatctat aaattctgaa
     2101 aggcttaacc tgtataaaga gatatatgat tctttgcttt catgtagatc atcaatgagc
     2161 gatcagtatc ttatcatggg tgatttcaaa tcatataaag aaacgcatga caaaatggtt
     2221 gctgattatg aagacagaaa cagctggaat aaaaaggcta ttataaatgt ggcaaatgcc
     2281 ggaagattct catcagatat tagtattgca aattataata aacggatttg gaaacttaaa
     2341 taatgctcga aattttttac aatagcaggg atactgtata taaatctata tttggagctg
     2401 tgcagtgtgc tacattgatt aaattcagaa ttgatgtaag atgtgatgct cctgtcaaag
     2461 cagccatcat aattaaccac ataagtcatg aaatgcagat agacagtctt actggggatc
     2521 tatctgtttt tacattatca ttgcattcat tgcacaaacc aggcttgatg tattatcatt
     2581 ttgaagtatc aaccccttac cataccgttt attatggtaa tgatatggat atgttacagg
     2641 gtataggaaa aaaatacgat aaaaagcctt tggactatca aataacagta tatgacaaat
     2701 ttaatactcc tgattggatt aaaggaagag tcatgtatca gatttttcct gacagattct
     2761 ataaagaaaa tataaagcaa agtccgtact gggaccgcta ttttcatgaa agttggtatg
     2821 aagacccgta tatagaagat gattttaaaa gcggtactat ggagtgttcc gacttctttg
     2881 gagggaattt agatggaatt attaagaaac tggattatat aaaatcatta ggagttggag
     2941 taatttatct taatccaata tatttggctt attcaaatca tagatatgat gtagcagact
     3001 acatgtcagt agatgatact ttaggtgaag agaacgattt gagaatatta tgtgaagaag
     3061 ctaaaaaaag agatatatac attattcttg atatggtatt ttcgcatact ggtagtgaca
     3121 gtgtgtattt taataaaaat atgacatttg gatatcaggg tgcttttaat gacaagcaat
     3181 ctgagtatag aagctggtac gtctttaaaa aagataatac atatgaatca tggtggggaa
     3241 ttgaaacttt accaaatact attgaaacaa atccgtcatt tatgagtcac acattatctt
     3301 ctcttgaaaa atggatgaat cttggtataa aaggatttag gcttgatgtc gcagatgaac
     3361 ttcctgatga atatattaaa gcattaagaa gtaaaattaa aagtatagat aaccaatcag
     3421 tattaatagg agaagtatgg gaagatgcat caaacaagtc aagttatggc aaaaaacggg
     3481 agtatctgta tggatatgaa ttggattcag tcatgaatta cccttacagg gatgccctta
     3541 taaactatac tttaggcaaa atagatgcga aaactttttg tagaaaaatt gaaataattc
     3601 gtgagaacta tcctaaacag gtatatgaat gtcttatgaa tatgctaagt acacatgatg
     3661 tttccagaat actttctgta ttaggaggaa aatcagattt tgaaaatata ccgagaaatc
     3721 agcaaaagaa tatttatcta aataaggagg aatatgaaat tgctgttaag cgtttgaatt
     3781 ttatcatatc tcttcttata tgtcttccag gagttccgac aatttattat gccgatgaag
     3841 caggcagtca agggttaaaa gaccctttta accgaaggac atatccatgg ggtaaagaag
     3901 atttaagaat ttttgagatt tataaaggtt tccttaatca aagaagttcg aatgaactgc
     3961 ttagatatgg gacattctct atatcggaaa aagaagataa tattcttatc aaaagagaat
     4021 atggtcaaga agtaagtact ttcattttca ataacaccaa attgtcatat ataaaggtat
     4081 aacacagttt ttgatgttgt acagtattac tatgtatgat agcatttaac cagatttgtg
     4141 aagggagatt attatgagca aaataacttt tatgggggca ggtagttctg tatttgtaaa
     4201 aaacgtaata ggtgactgta tgtgtacacc tgttttagct gattcagagt ttgcattgta
     4261 tgatattgat gaacaacgat taaatgattc aaaacttctt gttgatactc ttaataagaa
     4321 tattaataat aacaaggcta ctattaagtc ttatcttgga gtagaaaaca gaaaagacgc
     4381 actaagagat gctgatttta tcgttaatgc aattcaggta ggtggatatg aaccatgtac
     4441 tgtaattgat ttcgagatac caaaaaaata tggaattaat cagaccattg ctgatacttt
     4501 gggaattggc gggattatga gagcattgag gactattcca gttatgaaag agtttgctac
     4561 tgatatcgag caggtatgtc ctaacgcttt attattgaat tacactaatc caatgtcaat
     4621 gttggcagga tatatgcaaa gatatacaaa ggttaaaact gtcggtttat gccattcagt
     4681 acaggtatgc tctgaagctg tactaaacgg agttggaatg caagacaaac ttgaaggcag
     4741 agtagatttt attgcaggaa ttaatcatat gggctggctt cttgatataa aagataaaga
     4801 tggaaatgat ctttatcctg aaattagaaa aaaagctgct attaagaaca aaacagaaaa
     4861 gcacaaagat atggtgaggt ttgagtatat aaaacatctt gggtactact gtactgaatc
     4921 aagcgagcat aatgctgaat ataatccttg gtttataaaa cctaattatc cagaattaat
     4981 tgaaaaattt aatattccgc tggatgaata tcccagacgc tgcagaaacc aaattagcag
     5041 atgggctgaa caaaaacaag aacttttaaa taatggcagg atagaccatg aaagaagcag
     5101 ggaatatgca tcatacataa tggaagcagt agttacaaac aaaccattta aaattggcgg
     5161 taatgtaatg aatacaggat tgattgagaa tctgcctgct gatgcatgtg ttgaggttcc
     5221 atgtatgata gatgggaatg gaataaatcc atgtcatgtt ggcagactac ctgtgcagtt
     5281 agctgcaatg aatatgacta atataaatgt tcatcttcta acaattcaag ctgcggtaac
     5341 aaagaagaga gaagacatat atagggctgt aatgcttgaa cctagatgtg cagctatttt
     5401 gtctgtggat gatattataa agatggtaga tgagttaatt gaagcacatc agagagttgg
     5461 atttatgact gaatataaat aatccatctt tatatcattg acttaagttg cattttatta
     5521 tatatttatt gagatagcga attggaggtg gaccatggaa aatgcattat tacagactat
     5581 aacaggattc cttgtagtaa ttatagggat gctcatattg acagctgtta tcatcttgtt
     5641 ttcaaaatta atgggtaaga atagcactaa caaaacagtt gcagtcaaac aagttgaaaa
     5701 gcctgttaca cctgttgctg aaatatatgc agactttgat gatgaagaag aaattgcagc
     5761 tgtaatggca tgtatagagg catatcttca gcagaatagt ataaagaatg tttgtaatat
     5821 agtggtcaga cctttagtaa gaactgtatc ttcgtcaacc tggagtcagg ctggcaggat
     5881 taaccttgta aataagaaag tttagggcag gtatatatat gttactcgaa agtttaaaag
     5941 atttatttaa tcagtcagca tttgtaaata taacatggca gcaaggtata ctcataatag
     6001 tatcgcttgt tttgttatat ttaggaatag ttaagaagtt tgaaccttta ctacttgttc
     6061 caatagcttt cggtatgtta gccacaaatc ttcccggttc aaacgttttt aatcctgaat
     6121 tatgggaaaa tatgaagaat attaattatg gtgatgtttt aagaaatggc ggattactgg
     6181 atatattata tattggaata aaagttggtt tattccctcc acttatattt atgggcatag
     6241 gagctatgac cgatttcggt cctctgattg ctaatccaaa aagctttata ctaggagcat
     6301 cagcacagtt tggtatattc tctgcatatt tcttagcaac aaaattggga tttactgcag
     6361 cagaatcagg agcgattgga ataattggcg gagcagacgg acctactgcc atttatactg
     6421 catccatctt ggccccacat cttttaggag ccattgctgt tgcggcatat tcatatatgg
     6481 ccttagtccc agttatacaa cctccgttta tgaagatgct cactacaaaa aaggaaagag
     6541 caattgtcat ggagcaatta agacctgtaa gtaaaacaga gaagataata ttccctatac
     6601 tagttacaat cgttgttgca ttcttaattc caagcgcagc tgttcttgtc gcatctttaa
     6661 tggttggaaa cttgcttaag gaaagtggtg ttactgatag attgtcaaaa accttgcaaa
     6721 atgaatttat taacattata actatttgtc tcggccttgc tgtgggagct acagcaacag
     6781 catctaattt cttaaaagca gaaacgctta agattcttgg tcttggagta ttagctttca
     6841 tattcggaac tattacagga atactgtttg ctaaattaat gaatgttttc agtaagaaaa
     6901 aaatcaatcc tttgattggt tctgccggag tttctgcagt tcctatggca gcaaggatat
     6961 ctcagaaagt tgcacaaagc gaaaatccga atatattctt attgatgcat gcgatgggac
     7021 ctaatgttgc aggagttatt ggttcagcca ttgctgcggg agttttaatt tcacttcttg
     7081 gataaaagaa taacctaatc taatagagtt gaaagagtaa aaatataaaa tatagaacat
     7141 agaatgacag gggaaaccct gttatttttt aaaattaaca tattgaaaat attataaaca
     7201 tatgttaata taactaaaat gatttttgtt taatgaaata atgcaaaaaa tactaaatat
     7261 atgaatggag taaacgaaaa atgaaattta aaagtcctta tattgcaaga gtgtatgaag
     7321 acatagttgc aagaaatcct aacgagccag agtttcatca agctgtcaaa gaagtctttg
     7381 aaactattac acctgtaata gagaataatg atatatatga gaaacatgga atacttgaaa
     7441 gaataaccga accggaaaga cttataacct tcagagtgac atgggtatca gatgatggaa
     7501 aagtcaatgt taatagggga taccgtgttc agtttaattc agcaataggt ccatataagg
     7561 gaggacttcg tcttcaccct tcagtaaatg catctgtttt gaaatttctt ggctttgaac
     7621 agattttcaa aaatagtcta acaggactac caatgggcgg cggaaaagga ggttctgact
     7681 ttaatccaaa aggaaaatca gatatggaga taatgagatt ctgtcaggca tttatgtctg
     7741 agttatacag acacataggc ccaaatcttg atgtgccagc aggggatatt ggcacaggtg
     7801 ctcgtgaaat aggatatctt tttggacagt ataaaagact tgcaaatgaa tttaccggcg
     7861 ttttaactgg aaaaggatta gcctatggag gttcattggt tcgcaaagaa gctacaggtt
     7921 atgggttatg ttattttact gaagaaatgc ttaagggttt aaaaaatgat tcaataaaaa
     7981 ataaaaaagt atgtgtatct ggttctggca atgttgcaat atatgcaata gagaaaattt
     8041 atgaacttgg aggacttcct gttacagcaa gcgatagtga tggatttata tatgatccaa
     8101 atggtatcga tcttgattta ttgaagaaac ttaaggaagt tgaaagaaaa agaatatttg
     8161 aatatgctga tatagtaaaa ggagctgaat attttgaggg tcaacgacct tgggctattc
     8221 catgtgatat agcattacca tgcgctacgc aaaatgaatt agagctggat gatgcaaaaa
     8281 aattggttga aaataatgta attgcagttg cagaaggtgc aaacatgcct tgtacacctg
     8341 aagcaataga gtattttcag aatagtaagg ttctttatgg acctgcgaaa gcagcaaatg
     8401 caggtggagt tgcaacaagc ggattggaaa tgagccagaa ttccatgaga tatagttgga
     8461 cctttgaaaa agttgatgaa agattgaaag ctattatgaa aggtatattt gataatgtta
     8521 gtgaaacggc taaaacttat ggagttgaag gcgatcttat ggcaggagct aatatggctg
     8581 ggttcttaaa agttgcagat gcaatgattg cccaaggtat aattttttag taatattcgt
     8641 tttttaatat gcaaatttag aactgttcta aacagttcta tttttttgca gaattcaatt
     8701 tttactaatt tttaatgatc gttttaaaaa ttttattgat attttctgat aatatgatag
     8761 aatactgtaa acactatgaa gaaagaaagt acccttatct gatttttcaa agagagacaa
     8821 tgccttggct gaaagcgttg taaagagcaa tttggggaag ttccttttgg agtggtccgg
     8881 ttgaaaatta tatgtaggct ggaacggatg gacaccgata gaagtctcta gcattgttgg
     8941 atgcgtagtc tcatggtttg aatcagcttt cgccctattg ggatgaaggc tttatttata
     9001 tttacggagg aaaagaaaaa tgttacagct ggataatatg ttaataacag cacaaagcga
     9061 gatttcttca gcaaaagaca tgaagaggat tgaagaactg aaggttcaat acttaggaaa
     9121 aaaaggagaa cttactcaga tattaagagg tatgagttct ctttcacctg aagaaagacc
     9181 tgcagctgga aaagctgcta atgaagcacg cgaaaaactg aatatgctgt ttgatgagat
     9241 ggagcaaaaa atccagtata acgtacttaa tgaaagactg gaaaaagaga ctattgatgt
     9301 tacaatgccg ggagtaaaaa atgaaattgg ttctatgcat cctctttcac aagttataca
     9361 gaaaatagag gatatattta ttggattagg atatcagatt gcagaaggtc cagaaattga
     9421 aactgttcat tataatttcg atcaattaaa tacaccggaa aatcatccat caaaagatga
     9481 acaggataca ttctattttg atgcacaaaa gcttttaagg acacagacct ctcctgttca
     9541 agcaagggta atggaacgta caaatccacc cattagaata atatgtcctg gaagagtata
     9601 cagatctgat gagattgatg ctactcattc acctttattc catcaggtag aaggacttgt
     9661 tgttgataag ggaataacaa tggggaatct ggttgcgact ttaaaattat ttgcaaaaga
     9721 actatttggg gagtttacag agataaggct tcgtcctcat tactttccat tcactgaacc
     9781 aagcgcagaa gttgatgtaa gctgctgggt atgtaaaggt aaaggttgtc ctgtttgtaa
     9841 gtatgaggga tttattgaaa tactaggtgc aggtatggta catccaaatg ttttgagaaa
     9901 ctgcgggatt gatcctgaca tttacacagg cttcgcattt ggattaggtg ttgagcgtac
     9961 agctatggcg gtatttggta taaatgattt acgcttatta tatgaaaatg acatgagatt
    10021 cttgtctcaa ttctaggagg atatatatga aaacaccaat aagctggtta agagattttg
    10081 ttaaaataga tagagatata aaaagttttt gtgatttaat gacaatgagt ggaacaaaag
    10141 ttgaaggtta ttcaattgtt ggaaaagaga taaatcgtgt tgttactgga aaaattctag
    10201 aattatggaa tcatcctgat tctgatcatc tgctaattgc aaaagttgat gttaaagaat
    10261 ctgttcttca gattgttaca ggtgctccaa atgtcaaggt aggacaaata gtacctatag
    10321 ctttagataa ttcaactctg ccaaaaggta aaataatcag aaaaggtaaa ttaagaggag
    10381 aattatctga gggtatgatg tgttctgttc aggaacttaa tcttacaaaa catgattatc
    10441 ctgatgcttg cgatgatgga atatggattt taaaagaaga cactcctatc ggaatggata
    10501 ttaaaaaata tgcaggtctt gacgatactg ttgttgaatt tgaaataact ccgaacagac
    10561 aggattgtta ttgtattaaa ggtattgcaa gagaagcatc ggctgctctc aatgttccaa
    10621 taaaagaaaa acaaattgca ttaaaagagc agggaaaagg aaaatcttct gattatataa
    10681 aaataactat atcagcatac gatttatgtt caagatattg tggcaggatt ataactgatg
    10741 ttgtaatcga gccatctcct gaatggatgc gtcaaagact tagaaatgct ggagtaagac
    10801 ctataaataa catagttgat atcactaatt atgttatgct ggaattaggg cagccaatgc
    10861 atgcatttga cttaaactat attgaaggtg gtcttataaa tgtaagaaga gcatataatg
    10921 atgaattagt tattactcta gatgaaaaag aaagagaact tgacagttct atgctagtaa
    10981 tatcagatgc taaaaaaact gttgctgtag caggtattat gggagctatg aatagcgaag
    11041 taaatgagaa cactaaaaca gttctttttg aatctgctat gtttaatccg acaagcatta
    11101 gaataactgc aaagaagctt ggcatgcgta cagattcatc tgctcttttt gaaaaaggac
    11161 ttgatgcaaa taatactttg actgctatca acagagcatg tgagctagtt gagatgattg
    11221 gtgcaggtaa agtatcaaag ggaataattg atgtacataa agaattgcca atgccaaata
    11281 gtgtattgct tgattcagat aagataaata ctcttttagg tactgacatt aagataaatg
    11341 atatgaagtc aatacttaca agattaggta tgagtattaa gtcagataat acagtagttg
    11401 ttccgacttt cagaccagat gtttcaacat atgctgatct tgcagaagaa attgctaggt
    11461 tatatggtta caacaaaata gtaccttctc tgacattggg acaatcggta actcaaggag
    11521 caagaacgca tattcaaaat gtcagaaaaa ctattagtga aacattagtt tgttgcggat
    11581 tgtatgaaat aatgacctat tcgtttatca gccctaagaa tttcgataag cttggattag
    11641 atatgaataa tcctcttaga gattgcatgg ttataaaaaa tccattgggg gaagattttt
    11701 cagttatgcg aacaactact ataccaggaa tgctaaaggt attatcttac aatagcaaca
    11761 gaaatttaca aaaagcaaga ttttttgaaa ttggaaaaac atatcatcct agtgataatt
    11821 tagcaaaaga aatcgactat ataactgttg gcatgtatgg ctcatgtgat ttttacgata
    11881 taaaaggagt tgcagaaagt gtgctgaaaa gattagggat aacagatgtt ttttatagca
    11941 cgaatatgga aaatgaagta tttcataaag gaagatgtgc tgacataatc catgatggaa
    12001 gcaaaattgg tattcttgga cagatagatt acaaagtcac tgaagctttt gatgctcctg
    12061 atgaatcata tatccttatt atgccgattg aaaagttagc gcaaaagact gatttttcca
    12121 gaaacttcaa gcctttaccc aagtatcctt catcacagag agatctttca gtcgtagtta
    12181 aaaaatctat tttatctgca gatttactga acgagtcaaa gaaggttgca agggatattt
    12241 tagaggatgt tcagttgttt gacatgtata caggaggcca gattgaagag ggatataaga
    12301 gcctctcata tgcgttcaca ttcagatcaa ttgaaagaac tcttgaagaa gatgaagtta
    12361 ataaagcaat gtctgatatt ataaatattt tagaaaagaa atttgatgca aagataagat
    12421 gttagaggat gtatataaaa gatttgaaaa tgacaggttt gcttatggcc ttctgaactg
    12481 tgaaattgtt gagatttcag aggggaaggc ctcagcttca cttatggtaa agagagacca
    12541 tttgaatggt gttgatcttt gtcagggagg tgttatatat tcacttgctg atttttcctg
    12601 cgcaatggct gcgaattcat atggtccggt ctgtgtaacg cttgaaaata atattatata
    12661 taagagatca gttaaagaag gggaaatcat gattgcctca tcctttgtta ttgagagaat
    12721 gaagaaaata ctaatatgtg aatgtgtaat cacagtaaat catgagatca gggcaataat
    12781 gaagtccaag ttgttgagtg ttgagcaata atgtaatata ctttacatta aagaagatga
    12841 aaggcattgt tgatgttcaa taaaatcagc gtcaggacat tttttaaaaa gtttgattat
    12901 attcttttgg gattagttct tgctttaaca gcaataggta ttgttgctat gcctagtgtt
    12961 gttgatacta tgaattccgg tccgtcaatt cttaaaacgc aattattcag tatagcttta
    13021 ggattatttt tgtgcttggg attttccata ttggattact catttttgcg ctactggggt
    13081 attgcattct atataatagg tttattaatg cttatgtatg ttattccatt cggatatggc
    13141 agagataatg ttaatattgg atcgaatagc tggatagatt tattcggagt attcagtttt
    13201 caaccttctg aactaatgaa agtagcatat atgatgttcc ttccttctca atttgagctg
    13261 cttaaagagg aattttccct aaaaagactt atatttattg caatatcagg aattctacct
    13321 ataggtctta tccttatgca gccagactta ggaactgcaa ctgtttttgc tttttctttt
    13381 gcagtactaa tatttgtata tggtattaaa tacagatata taattttatc tttactggct
    13441 ttgggtattt ccttgccttt tatttggatg tttttggatg actggcagaa agataggatt
    13501 cgtgttttta ttgatccgac ttttgatcct actggtgcag gatatcagac atcaaaatct
    13561 atcgttgctt taggtagcgg aggatggtct ggagcaggtc tttttaatgg tattcaaact
    13621 cagggtaatg gaatacccat taaagaatca gatttcattt tttctgcaat tggagaagaa
    13681 ttgggattca taggatgtgc tgctatagtg ttattagcct ttgggattat tatcagatgt
    13741 atatacattg catcaaaatg tgcaagttat tacggccagt tcatagttgc aggtatgtct
    13801 gcaatgatag ctttccattt tatagaaaat attggaatga atataggact tcttcctgta
    13861 acgggaatac ctctgccatt tatatctgca ggaggcacaa atcttatagg aagttttgca
    13921 atgattggta taataatatc tgcttcaatc agaagagagc aggttaaaag gttgtaaata
    13981 gtaaaaagag tgatttatgt gacgtctctg aaagaggcgt tttttattgt gaaaatgtaa
    14041 aaaaagtgtg aaaatagtat tgacaaaggc aggaaatgag gtgtaaaatg taggcaagtg
    14101 gttacaaagt ggtgtataag tggttattta gtgtattgga gataagctga tggcaatgta
    14161 tggtgaattt acacataggg tggatgataa gggcagagtg attcttccag tatctatgcg
    14221 tgaagttctc ggagacaaat ttgtaattgc taaaagttct gacagtacat tgaaaattct
    14281 atacctttac acaagagaag attgggaaaa aataattgaa gctggtgcaa gaatagcagc
    14341 aaaagacaga agtaaagcaa catatgtaag acaactatct gcaagtggta aagacactga
    14401 acttgatagt cagaacaggg tactgattcc aattgaattg agaaattacg caaacatagg
    14461 taaagaaaca gttttattag gattaggaaa cagagttgag atttggaata aggaatctta
    14521 ttacattaat catcctgatg aaacagaagt aactaaagaa aaagctcaag aagatgctga
    14581 taaagtagct tcacttcttg aagaaatagc actactgcaa tgaaagagta ttcacacgaa
    14641 tcggtaatgg ccgatgaatg tattgaatat ctgaatattg acaaaagagg aacttacatt
    14701 gattgcactg ccggcggagg cggtcactca gtaagaatat acgaaagatt aaacgaggat
    14761 ggattgctta tagcctgtga tcaggatatc aaagcatgtg aagtaacaaa agaaagactc
    14821 gaaaagtaca acagaaaaac gaaatttata gtattacatg aaaatttcgt gaacattgac
    14881 aaggtaatat cagaatattg tccagaaggt gcagatggaa tactacttga tttgggagtt
    14941 tcctcatatc agttggacaa cgaagaaaga ggcttctcgt atcatatgga tggaccatta
    15001 gatatgagaa tggatcagac aaaggataaa accgcatatg atgtggtaaa taattacact
    15061 aaagaagaac tttcccaaat actatttgaa tatggggaag aaaaacactc aagaagaata
    15121 gcagaagcta tagtacacaa aagaaaagat gaactgataa gtacaacaaa tcagcttgca
    15181 gaaataatta aagaagcata tcctgcgaaa gaaagattta aaggtaagca tcctgcaagg
    15241 aaaacatttc aagcgattcg tatagaagtt aataatgagc ttacagtttt aaagcaaatg
    15301 ttaccaaaag cagcattttc actaaagagt aaaggcaggt tagcagttat aacattccat
    15361 tctttggagg atagaattgt taaaaatgct tttaaggagt taattgatcc gtgtacttgc
    15421 ccgaaatcct ttccggtatg tgtttgtaac aaagtggctc agcataaatt aattgttaaa
    15481 aaaccgcttg taccgaaaag tgaagaaaca caaaagaatt acagatcgag aagttctaag
    15541 ctaagagtgc ttgaaagaac ttaaagatat acactaaaga taaagcgttt acgctaaaac
    15601 actcaagaaa tcttaaaaca ctaaagaaaa ggaaagccgg ttgtaaaaat tccccctttt
    15661 acaaccggta ggtctttgac cttgaatgaa gaatggaaat tgaaagaaga aatgaaaata
    15721 atataattgg taaattgaag gctgtgatac tgcttatgct tgtattcaca gctttatttt
    15781 taataatttt cagatatggc gagatgataa acctgaatta tgatattacc agacttaata
    15841 atgaattgag agaaaaaacg ggtatcaatt ctgctttgta tgctgaattg gataaacagg
    15901 ttactattaa tgatatcaag tacactgccg aaactaaact tgatatgaat aaaccaagaa
    15961 atgatcagat aatatacata aataccaaat taagtgatga ttataattca caagaaaaag
    16021 ataacttcta tactatgact tcttctgttt atgaaagaat aaatcatacc tttaaaaata
    16081 tagttgagat atttaagggt tcaaatgagt gaaaaaatat tgtatgattc aataagcggt
    16141 ttaataatat gaatgatata gaaaacaaaa gaagtaataa aaaagaaaat gtaaaaactg
    16201 aagttgtata caataataat ctaactagaa aaaggctatt agttcttatg gcagttttca
    16261 ttataattgc aatggttatt gcaggatata caggatatat tcagcttgtt tgggcaacag
    16321 aacttaaagt aaaagcatat gagcaacaaa atcaagatag aacaataaaa gcaagtaggg
    16381 gaacagttta cgattgcgaa ggaaatgttc ttgcaatatc aataagtgta gatactattt
    16441 cgataagtcc tgtaacagtg cagaagaata aagatataac tgttgaagaa atagcttcag
    16501 atttaagtaa catattgaat cttgaatatg aatcaacact taataaattg aaaagtgaaa
    16561 gccaatatca gcttattgca tcaaaagttg acagacaaat tggtgatgtt ataagagatt
    16621 atattaaaac taatagtgtt gctggcattt atgtacagca agatagtaaa cgatattatc
    16681 catatggcag cctcgcttca catgcaatcg gatttgcagg tgatgataat cagggattat
    16741 ttgg
//
LOCUS       DAOSXM010000046        16027 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2111
            NODE_2286_length_16027_cov_10.147696, whole genome shotgun
            sequence.
ACCESSION   DAOSXM010000046 DAOSXM010000000
VERSION     DAOSXM010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864178
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 16027)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16027)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:24:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,261
            CDSs (total)                      :: 2,229
            Genes (coding)                    :: 2,218
            CDSs (with protein)               :: 2,218
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 3 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16027
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2286_length_16027_cov_10.147696"
                     /isolate="AMR_MDS_2111"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..383
                     /locus_tag="PLQ68_06925"
     CDS             <1..383
                     /locus_tag="PLQ68_06925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HXK72422.1"
                     /translation="SEINNLLSGMNDDVPKEQLKAVLFAQHYAENVGKASLQTWQKIV
                     IEYGNNAALSILASIRMIMMGNAYGIPFSLLINRFKKDRNKDERSNLFYEISTILISI
                     LIIPVSAIHAFINCLSGYKYVDFK"
     gene            537..1859
                     /locus_tag="PLQ68_06930"
     CDS             537..1859
                     /locus_tag="PLQ68_06930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72423.1"
                     /translation="MRKVLSLIVLLLLVIGISGCSTEELSKNSETPTQSIQIENQDNN
                     EDSLDIYSMDDVYLFLHSVYPEYSKDNFEKWNVSYLDVTEDGFDDVIFTSTYGDGELE
                     SAIIITAEKDGFREISRYIPLSKYANNMEMKDGLLINNTRTGGSGSFSEYMDIYSYDG
                     QYINVSRQNILVKETVENPSANYEISSTIEGNLEDFIITYTKKNFDDSQCYVIAKDRY
                     VLNENLEYNKIPISLDNSALGFDSVFTGENLLETIQYFNDNLYLFEDELRVNYAKEIF
                     NVLEEEKMVMTEWGNFITIPAEYVNLETMNYDISIMKSNEPETIILEKIKNSNIFTLV
                     KVYYADQGLVQDNGFNVIINGYVYSMLQPAYEIMCSNDESFNLPFLKPDIYFTSSYMS
                     EKVVTMTHIVYPNVVVENIDDIEFFEGKDTWKTNLLVRLNIVGKPVTP"
     gene            1975..2442
                     /gene="bfr"
                     /locus_tag="PLQ68_06935"
     CDS             1975..2442
                     /gene="bfr"
                     /locus_tag="PLQ68_06935"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019178640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HXK72424.1"
                     /translation="MKGNAKLLEVLNSLLADELAAINQYIVHAEMCADWGYEKLHEHF
                     EKRAMDEMRHAEKLIERLLFLEGIPIVSELSNIHIGSDVIKQLEYDRVSEMAAIKAYN
                     DAIALAGEVRDYATREVLDKILQDEDRHMDGIEELQGQIEHMTLPIFLTTKVV"
     gene            2509..2787
                     /locus_tag="PLQ68_06940"
     CDS             2509..2787
                     /locus_tag="PLQ68_06940"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012198195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXK72425.1"
                     /translation="MNEIRKGKEMFYVGKSEEEYLAVITYKKVDDKTIKADHTFVSEQ
                     LRGQKIAKKLLNELVSFARENGYKIIPECSYVKKVFENSTEYRDIIKI"
     gene            2817..3506
                     /locus_tag="PLQ68_06945"
     CDS             2817..3506
                     /locus_tag="PLQ68_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013658905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HXK72426.1"
                     /translation="MKTIGLIGGMSYESTVTYYNIINSRINNALGAHHSAKILLYSVD
                     FEELYSKMKKNEWQEISSILINAAKSLEIAGADFILICTNTMHMVFDKVQEVLDVPLI
                     HIADALIDRLSGDNIKKVLLLGTKTTMLQPFYTDKLFKSDIEVILPSDNMMNELDNII
                     FNELCVGNINDISKEKFISIIEAGMKKGVKACVFGCTELGLLVSESEIDIPVYDTAII
                     HSIAAADYAIL"
     gene            3563..4564
                     /locus_tag="PLQ68_06950"
     CDS             3563..4564
                     /locus_tag="PLQ68_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003528231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HXK72427.1"
                     /translation="MREGITIAGNMIMDILYPINGLPKPGELTTILDGITRATGGAVC
                     NVVTDLARLDQSLPLYAIGRVGSDQEGDNILEELRKYDNIDTSDVKREGITSFTSVMA
                     DTITKQRTFFHYRGANATLDISDFDFSKVCSKIFHIGYILLLDSLDQEDDEYGTKMAK
                     LLHNAQKKGIWTSIDVVSEASDRFKRLVPPAMKYADFCIINEVEAQNSTGVVLRDNHD
                     MLLTKNIPSALAEMKNMGVSRWAVIHCPEGGFGLDENNKYVNVPSLDLPEDFIKGTVG
                     AGDAFCSGVLYAAHQEKSLFEAIEYGTACAACSLSKPGASDGVLPINEALELYESLK"
     gene            4576..5454
                     /locus_tag="PLQ68_06955"
     CDS             4576..5454
                     /locus_tag="PLQ68_06955"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HXK72428.1"
                     /translation="MEIGVQTIGYGKFIDERQAAKLVAQAGFTVLDYWLEEESFLKLP
                     KKEFKKHFEDMRKHINECGLTVGQTHAPFYPYSKHMQNYDEVLELQKRSIAATAFLGA
                     KYVIVHPLRPKNRVFDQAIEETFELNKMYYKEFIPLMEEYDVNICIENLYTADENKFV
                     IPSTCSTAEEMVMYIDKFGDRFYACIDTGHVNLVHREGYEHVNFKHMMKILGDRLKTL
                     HVHDNNGLVDQHYIPYLGTIDWNEFVDALRDNNYDGNLSLESFTFMNKINRDNIDEGL
                     KMMYMAAKRLYDRFVR"
     gene            5458..6339
                     /locus_tag="PLQ68_06960"
     CDS             5458..6339
                     /locus_tag="PLQ68_06960"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HXK72429.1"
                     /translation="MKIGAQSAVIDRVYGPEKALSIVRSAGFETIDFSLESETRLYLH
                     STALKKYFENIKKHAEDIGMELAQTHAPFYNPDKHMENNDEIIETQKRAIAATGHMGS
                     KYIVIHPMKTKERMYNDDYERTKELNLKYYTALIPTLKEYGVYLAVENMFTWDSTLKC
                     ICPTSCTTAEEMVEYIDELGEMFVACLDTGHVNLIHQEGFEHVNFPHMIRTLGNRIKI
                     LHVHDNDGIKDLHLPPFAGNIEWVEVMDELKKIGYLGHISLEADNYMTYVKPEQFENA
                     EKYMYSTAKRLYDMIIE"
     gene            6367..7242
                     /locus_tag="PLQ68_06965"
     CDS             6367..7242
                     /locus_tag="PLQ68_06965"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HXK72430.1"
                     /translation="MKIGTQSKEIDKVFGPHQALLILKNAGFEAVDFSLENEERLKKD
                     DSELLVFFEDIKKYADEIGIEIGQTHAPFYNRENQMNRFDEIVKLQINAIKATAFLGC
                     KYIVIHPLQSEGRIYDLELEETKKLNMKYYSALIPALLEYDVYCAIENMFARDPEKRI
                     WCPTTCTTAEEMVDFIDSLGERFVACLDIGHINLIHQKEYPHVSFEHMINTLDYRLKV
                     LHVHDNNGKDDQHLPPFAGNIDWSAVVKALKKIGYAGNLSIEADTFITYIKPEQFEKA
                     ENVIYAYAKRLYDMF"
     gene            7255..8367
                     /locus_tag="PLQ68_06970"
     CDS             7255..8367
                     /locus_tag="PLQ68_06970"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HXK72431.1"
                     /translation="MNKIIRGKEILTSKERIQRTFAFEDVDRVALGYDTNPVAHENLC
                     KVLNLDSKDRNAFNKHLGIDHMGARIKYNGPKIYEDVPQRTRNSEHGAITKYVQNQFG
                     AYWDFCDFPLEDADDDVIYNYPYPSADDYDYKACADYVDYLVKEGFAIHVGDPGLGDI
                     LNTTGMIMGVEQALINIATEHEPTIHTIDKRLNLQLKRTEKILEMNKGKIDFMWLGED
                     LGTQHTPMISMELYRKVLKPRHQKFIDLAKSFNLPVIIHTCGSSSWVYEEFIEMGMNG
                     VDTLQPEAVNMSPEYLVEHFGGRLVFRGCISTAGPLAYGTQKETERVCIDTLEIMSKC
                     KGYIFAPTHSIQDNTPPENTITMYNTAYSFKKGGSK"
     gene            8364..9251
                     /locus_tag="PLQ68_06975"
     CDS             8364..9251
                     /locus_tag="PLQ68_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005979550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - cellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HXK72432.1"
                     /translation="MKSVSISNKTMKILIKSQRDEETGALVYEYLSKKEKDPKNSEIL
                     NKMSKDEKSHAMTWKNYTKKNVKPDKMKLAFVKFISILLGYTFVLKIIQKDEGFAQKE
                     YEIIKQEIPEALKIQEDEHTHEMDLITMLDEERLQYVGAMVLGLNDALVELTGAIAGL
                     SFALANTKIVALSGIITGISATLSMAASNYLAEKADGNPNAFKSSIFTGVAYLITVAL
                     MVMPYLLLPVNAYVEAFLIMIGVVILIILVFNYYISVAKDQPFFKNFAKMAIISISVA
                     GISYLIGILAKALLGIDVI"
     gene            complement(9383..10417)
                     /locus_tag="PLQ68_06980"
     CDS             complement(9383..10417)
                     /locus_tag="PLQ68_06980"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007131233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HXK72433.1"
                     /translation="MALEKYVVSNDKSIYEAWPDIVKTKSGKLVCVFSECKHHGDRDQ
                     ARIVYKESLDKGKTWSDKTALTEKSTSDNYYNCARISKLPDDTLVIIADYVIKNESHN
                     AKTYLWKSIDDGKTWKGPFDTPSQGIVPDKLLVLKSGRWILSSHFLDISINKLVQYLW
                     YSDDSGITWSDRITVAKDEKYNLCEASILEVEDNVLIAFLRENSWEGYDCFKAFSYDG
                     GETWDGVYKIPIPGCHRPVTGFLSDGRILMTYRFIQGGKQGWGRTTQNFMGCIFDKQT
                     ALEKERRQQQTRIFPIDYDRNPKADLGYSGWVQFEDGTIYVVNYLLDDEPKGQIRGYT
                     FKIEDFLFSE"
     gene            complement(10419..11522)
                     /locus_tag="PLQ68_06985"
     CDS             complement(10419..11522)
                     /locus_tag="PLQ68_06985"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HXK72434.1"
                     /translation="MIRPKTEHVKIFKSKDSIFGYHGWPSVCRGLNGELISVCSGNRL
                     GHVCPFGKVLLSRSYDEGKNWTAPEIIIDTPLDDRDAGITVFNKDKIIVTSFNHLIDV
                     QSKFPAEGFKLAMRDAYFSNNQITKQMEKKYFGSTYVISEDKGITWSEVKHAPVTCPH
                     GPTALKDGGLIYMGYYKEVDDNPSKYDEKLRFKKELLVFYSKDGLKWDYLSHVPYVTD
                     EGNEYLHCEPYILELEEKLVGFIRVQGGIFATYMTESYDKGKTWTVPVRINKHGAPAH
                     AIEHSCGAVVLVYGYREQPYGQRAMVSYDRCKTWSDEIILRDDGPIHDLGYPASVELN
                     DGKLLTVYYQKETLDSQCEIFGTFWELPEKEGK"
     gene            11736..12665
                     /locus_tag="PLQ68_06990"
     CDS             11736..12665
                     /locus_tag="PLQ68_06990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72435.1"
                     /translation="MKLTNFIENGKFYKSNFHAHSTRSDGKLSQEDAINVFKEHGYNF
                     LCLSEHNIVTKTDKYNTDNFLLVPGLELHSLLEHPLTIHHMLGLTSMDNDKVYDGMKL
                     YNIPYTNNEESLLALEKTLTDLDLLTVYCHPTWSHVEPYQYDFLKNTNIVEICNWGTV
                     FEQNHNNDPTYWDSWLKQGKQIWGMANDDSHKREQYCGGWNMVKCEEFTIPSMLDSLK
                     KGSFYSTTGPQIHDFYIEDGVAYVKCSEAKSITFITLSNWGKCYRDPAGKLTEASLNL
                     PFNAYMTRVEIEDKDGKIAWSNPIMRMDRFEHR"
     gene            12678..14837
                     /locus_tag="PLQ68_06995"
     CDS             12678..14837
                     /locus_tag="PLQ68_06995"
                     /inference="COORDINATES: protein motif:HMM:NF013239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HXK72436.1"
                     /translation="MKVYKYRKGYLCITNISKNAIRLRTSLVNDFNEINPCVRYGIYD
                     MAEKTVEDSEISILIENDQLSINGMKTEILFNKGFDFKIKVADDRFYGLGDADRSTLE
                     KRGTRHVIWVQNVKCYIPYPMLLSSNGWGIFSSTTFKQVFDICSKESNTLSISSAKGS
                     CDIVIFTGNGFDELLSQFKSITGQSKLLPRFAYGLSYVANQKIDAFNMINEMYTFRKE
                     DIPCDVYGLEPEWMEKRYDSSVDKKFNTTKFYIPYWMDVNTIRQNTFFKPISNMDFKL
                     SLWLCCNYDLSYEEERTAVIKAHEISKQETDNEDDLSYLDERFEVDDHLGNPQYMDKL
                     TVKEEPWFKHLEKFVDMGVKAFKLDGAWQVDEHPDRLYGNGMTDEQMHNLYPLIYAKQ
                     MSSGYENHTNTRSLVYSSGGFAGIQQYVATWSGDTGGGPKPLVSMLNLAMSGHVNTTC
                     DMNVHSLEGIHFGFLQPWSQQCNWDYWNQPWLFTKDLKEAYTYYDKLRYSLIPYIYSA
                     AYEAYLNIAPIMRPLVYDYPEDENTFNILNQYMLGKYLLVAAFIDKDIYLPEGIWIDL
                     FTQKEYQGNRYIQYVVPQGRGGALFAKKGAILCRSYGISSISDEPFDSYTIEIYPSVT
                     ENEFVIYEDDGISLEYINGARCETCIKAFKRDDDINISISERKGEFTGKKDNVEYEIN
                     VMNVNKSDVIYINGVFWEYKIVGEDTAVIKINEKELF"
     assembly_gap    14855..14954
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <14955..15770
                     /locus_tag="PLQ68_07000"
     CDS             <14955..15770
                     /locus_tag="PLQ68_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015514619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HXK72437.1"
                     /translation="GQTEHPFTINFNSNDVRITTHYHKNNLLSSMFSVIHEGGHALYE
                     LNCDDEYDFTALSGGTSMGIHESQSRFFENIIGRSRSFTNYLYPYLKKMFPVQFKDVT
                     KVMLYEALNKSQPSLIRTEADELTYSLHVMVRYELEKRLINGSLSTKDLPAEWNRLYK
                     EYLGVDVPNDTKGVLQDSHWSGGAIGYFPSYSLGSAYASQMLFHMKKDLDIDTLTQQG
                     NLKPIVEWLKERVHKYASSKTPAEIIQICCNEDFKPHYYTDYLKMKFSSIYEI"
BASE COUNT         5584 a         2317 c         3058 g         4968 t
ORIGIN      
        1 aaagtgaaat aaataatctt ttatcaggca tgaatgatga tgtgccaaaa gaacaactta
       61 aggctgtact ctttgctcag cattatgcag aaaatgtggg taaagcaagt ttgcagacat
      121 ggcagaaaat tgtgattgaa tatggcaata atgctgcttt atcaatacta gcaagtatta
      181 gaatgattat gatggggaat gcgtacggaa tcccattcag tcttttaata aacagattta
      241 aaaaagacag aaataaggat gaaagaagca atttgtttta tgagatttca acaattctga
      301 tatcaatatt gataattccg gtatctgcga ttcatgcgtt tataaactgt ctatctggct
      361 acaagtatgt agattttaag taggctatca ttagttaaaa ttaaattctg aacctgtcat
      421 ttaatgtgaa atactgcaga ttcagtatta agtatctaaa atatcaacta tatccttgaa
      481 taatcagatg gttttctgta tactttcaaa gaataaaatt ttccggaggt tatagtgtga
      541 gaaaagtatt aagtttaata gtattactgt tattggtaat aggtatttca ggatgttcta
      601 ctgaggaact gtcaaaaaat agtgaaactc caacccaaag tatacaaata gaaaaccaag
      661 acaataacga agatagtctg gacatatata gcatggatga tgtgtattta tttttgcatt
      721 ctgtttatcc tgagtattct aaggataact tcgaaaaatg gaatgtgtca tatcttgatg
      781 ttacagaaga cgggtttgac gatgttatat ttacctctac atacggtgac ggtgaacttg
      841 aaagtgcaat tataattact gctgaaaaag atggttttag agaaatttcc agatacattc
      901 ctctttcaaa atatgcaaat aatatggaaa tgaaagatgg tctgttaatt aacaatacaa
      961 gaacaggtgg ctctggttca ttttcggaat atatggatat ttatagttat gatggtcaat
     1021 atataaatgt ttccaggcag aatatcttag ttaaagagac agtagaaaat ccaagtgcca
     1081 attatgaaat atcaagcacc attgagggta atttagaaga ttttattata acatacacaa
     1141 aaaagaattt tgatgatagc cagtgttatg taattgctaa agacagatat gtattaaatg
     1201 agaatctgga atataataaa atacctataa gtcttgataa ttcagcttta ggatttgata
     1261 gtgtttttac tggagaaaat ctcttagaga caatacagta ctttaatgat aatctgtatt
     1321 tattcgagga tgaattaaga gtaaattatg caaaagagat atttaatgtt cttgaagaag
     1381 aaaaaatggt tatgacagaa tggggtaact tcataacaat tcccgcagag tatgtaaatc
     1441 tcgaaactat gaattatgat atttctatca tgaagagtaa tgagccagaa acaataatac
     1501 ttgaaaaaat taaaaacagt aatatattta ctctggttaa ggtatattat gcagatcagg
     1561 gattagtgca ggataatgga tttaatgtaa ttattaatgg ttatgtatac agtatgctcc
     1621 aaccggcata tgaaattatg tgttcaaatg atgaatcatt caatttgcca tttttgaagc
     1681 cggatattta cttcacttcc agttatatgt cagaaaaagt agtaacaatg acccatatag
     1741 tgtatccaaa tgtcgtagtg gagaacatag atgatataga attttttgaa ggtaaggata
     1801 cctggaagac aaatctacta gtaagattaa atattgtagg aaagcctgtt actccatagg
     1861 acttcttatt gacttattaa ctctttctgg tttcgaaaag aataaaaaag atggtaatat
     1921 cttatactaa atgatattat atattcataa gaaaattgaa aggagatgtg tgttatgaaa
     1981 ggtaatgcca aacttttaga agtgttaaat tcacttttag cagatgaatt agcagcaata
     2041 aaccagtata tcgtgcatgc tgagatgtgt gctgactggg gttatgaaaa acttcacgaa
     2101 catttcgaga aaagagcaat ggacgaaatg agacatgcag aaaaacttat tgagaggctt
     2161 ctttttcttg aaggaattcc aattgtcagc gaattaagca atatccacat aggaagtgat
     2221 gttataaagc agcttgagta tgacagggtt tcagaaatgg ctgcgataaa agcatataat
     2281 gatgctatcg ctttagctgg tgaggtaagg gattatgcaa caagagaagt tctggataaa
     2341 attctgcagg atgaagatcg ccacatggat ggtatagaag aattgcaagg ccagattgaa
     2401 catatgactc tgcctatctt ccttaccact aaagttgtat aaaactatta cgaataagaa
     2461 catttaagcc ggttgaattg ccagccggtt tttatttgga gaagaaaaat gaatgaaatc
     2521 agaaaaggta aagaaatgtt ttatgtgggt aaatctgaag aggagtattt agctgttatt
     2581 acctataaga aagttgatga taaaacaata aaagcagatc atacatttgt ctctgaacag
     2641 ctcagaggcc agaagatagc taaaaaactt cttaatgaac tagtttcttt tgcaagagaa
     2701 aatggttata agattatacc agaatgctca tatgttaaaa aagtatttga gaatagtacg
     2761 gaatataggg atataattaa gatataaata atttactatt tacaaatgga ggaatcatga
     2821 agacgatagg tctaataggc ggtatgagtt atgaaagtac agttacatat tacaatatta
     2881 taaacagcag gataaataat gctcttggtg ctcatcattc agcaaaaata ttactctata
     2941 gtgtagattt tgaagagtta tattcaaaaa tgaaaaagaa cgaatggcag gagatatcat
     3001 caattttaat taatgcagct aaaagccttg aaattgcagg agcagatttt attctcatat
     3061 gtactaatac tatgcatatg gtatttgata aagtgcagga agttttagat gttcctctta
     3121 ttcatatagc ggatgctctt attgatagat taagtggaga taacataaaa aaagtactgc
     3181 ttttaggaac gaaaactact atgcttcagc ctttttacac tgataagctt tttaaatcag
     3241 atattgaagt tattcttcct tcagataata tgatgaatga attggataac attatcttta
     3301 atgaactatg tgtaggaaat attaatgata tatcaaaaga aaaatttatt tccattattg
     3361 aagcaggaat gaaaaaaggt gtaaaggcat gcgtattcgg gtgtactgaa ttaggattac
     3421 tggtttcaga aagtgaaatt gatataccag tatatgatac agcaattata catagtatag
     3481 ctgcagcaga ttatgcgatt ttataagtaa tcaggttgta tgcttttcat cagtgatata
     3541 tactttataa atggaggatg atatgagaga aggtatcacg attgcaggaa acatgattat
     3601 ggatattctt tatcctatta acggtttacc aaagcccgga gagttaacaa caatacttga
     3661 cggaattact cgtgctacag ggggagcggt atgcaatgtg gttactgatc ttgcaagact
     3721 agaccaatct cttccactgt atgcaatagg cagagtgggt tctgaccaag aaggtgacaa
     3781 tattctagag gaactgagaa agtacgataa catagataca tccgatgtaa aaagagaggg
     3841 aattacatca tttacatcag taatggctga tactattaca aaacagcgta ccttctttca
     3901 ttacagaggc gcaaatgcaa cgctggacat aagtgatttt gatttttcaa aagtttgttc
     3961 aaagatattt catatagggt atatactgct tcttgattcg ctagaccaag aagatgatga
     4021 atacggaaca aaaatggcaa agcttctaca caatgctcaa aaaaaaggaa tatggacatc
     4081 tattgatgtg gtatcagaag cttctgacag atttaaaaga ttagttccac cagcaatgaa
     4141 atatgcggat ttttgtatca taaatgaagt tgaagcacag aatagtacgg gtgttgtttt
     4201 aagagacaat catgacatgc tcctgacgaa gaatattccc tcagcattag ctgaaatgaa
     4261 aaatatgggt gtttcaaggt gggcagttat acattgtcct gaaggaggat ttgggttgga
     4321 tgaaaacaat aagtatgtaa atgttccaag tttggatctt cctgaagatt ttattaaagg
     4381 gacagtagga gcaggagacg cattctgttc gggtgttctt tatgcagcac accaggaaaa
     4441 atctcttttt gaagctatag aatatggtac tgcatgcgct gcctgttctt tgtcaaaacc
     4501 tggagcaagt gatggtgtac tgcctataaa tgaagcttta gagttatatg aatcgctaaa
     4561 ataaaggaaa cgattatgga aattggagta cagactatag gctatgggaa atttattgat
     4621 gaaagacaag ctgcaaagct tgtggctcaa gcaggattta cagttttaga ttactggtta
     4681 gaagaagaaa gttttttaaa acttccaaaa aaagagttca agaaacactt tgaagacatg
     4741 agaaagcaca taaatgaatg cgggctgaca gtagggcaga ctcatgctcc tttttatcca
     4801 tatagtaagc atatgcagaa ttatgatgaa gtacttgagt tgcaaaaaag atcaattgct
     4861 gcaactgcct ttttaggagc gaaatatgtt attgtgcatc ctttaagacc aaaaaacaga
     4921 gtatttgatc aggctattga agagaccttt gaactcaata aaatgtatta caaagagttc
     4981 attcctttga tggaggagta tgatgtgaat atatgtattg aaaatttata tactgctgat
     5041 gaaaacaaat ttgttattcc ttctacctgt tcaactgcgg aagagatggt aatgtatatt
     5101 gataaattcg gcgatagatt ctatgcctgc attgatacag gccatgttaa tcttgtgcat
     5161 agagaaggtt atgaacatgt aaatttcaag cacatgatga aaatcttagg tgacaggctg
     5221 aagactcttc atgtacatga taacaatgga ttagttgacc agcattatat tccatatctg
     5281 ggaactatag actggaatga atttgtagat gcattaaggg ataataatta tgatggaaat
     5341 ctaagtttag aaagtttcac atttatgaac aagataaatc gggataatat agacgaaggc
     5401 ttgaaaatga tgtacatggc cgctaaaagg ctttatgaca gatttgtgag gtaaaatatg
     5461 aaaattggag cacaaagtgc agtaatcgac agagtatacg gacctgaaaa agctctttca
     5521 atagtcaggt cagcgggatt tgaaacaatt gatttttctt tagaaagtga aacaaggttg
     5581 tatcttcata gtacagcatt aaaaaaatat ttcgaaaata ttaaaaaaca tgcagaagat
     5641 atagggatgg agctagcaca gacccatgct cctttttata atcctgacaa gcatatggag
     5701 aataatgacg agataatcga gacccagaaa agggcaattg cggctaccgg tcatatgggt
     5761 tcaaagtaca tagttattca tcctatgaag acaaaagaaa gaatgtacaa cgacgattat
     5821 gaaagaacta aagagttaaa tttaaaatat tacactgccc ttatacctac attgaaagaa
     5881 tatggtgttt atcttgcggt tgagaatatg tttacctggg attcaacttt gaaatgcata
     5941 tgtccgacat cctgtactac tgcagaagaa atggttgagt atattgatga actgggtgag
     6001 atgtttgttg catgtttaga tactggacat gttaatctaa tacatcaaga aggttttgaa
     6061 catgtaaatt tccctcatat gataagaaca ctaggtaaca ggattaaaat acttcatgta
     6121 catgacaatg atggtataaa ggatctgcat cttccacctt ttgcaggaaa tatcgagtgg
     6181 gtggaagtta tggatgaact gaaaaaaata ggttatttag ggcatattag tcttgaggca
     6241 gataattata tgacatatgt aaaacctgag cagtttgaaa atgctgaaaa gtatatgtat
     6301 tcaactgcaa aaaggttgta cgacatgatt atagaataga acataaaaag tataagaggt
     6361 gcaaaaatga aaattggaac acagagtaaa gagatagata aagtattcgg gccgcatcag
     6421 gctttgctta tattgaaaaa tgctgggttt gaagctgtgg atttttcttt agaaaatgaa
     6481 gaaaggctaa aaaaagatga ttccgaactt ttagtatttt ttgaagacat taaaaaatat
     6541 gcagatgaga taggtataga aattggtcag acccatgctc ctttctataa ccgagaaaat
     6601 cagatgaaca ggtttgatga aatagttaag ctgcagatta atgctattaa ggcgacagct
     6661 tttcttggct gtaaatatat agttatacat cctttacagt ctgaaggaag aatatatgac
     6721 ttagaacttg aagaaacaaa gaaactgaat atgaaatatt atagcgcact aatacctgct
     6781 ctcttggaat atgatgttta ttgtgcgatt gaaaatatgt tcgcaagaga tcctgaaaaa
     6841 agaatctggt gtcctactac ctgcacaact gctgaggaaa tggtggattt cattgacagt
     6901 ttgggggaga gatttgtagc atgtctggat atagggcata taaatcttat acatcaaaaa
     6961 gaatatcctc atgtcagttt tgagcatatg attaatacgc tagattacag actgaaagtt
     7021 cttcatgttc atgataataa tggaaaagac gatcagcatc taccaccatt tgcaggcaat
     7081 atcgactggt cagctgtggt aaaagcactg aaaaaaatcg gttatgctgg aaacttaagt
     7141 atagaagcag atacattcat tacttatatt aaaccagaac aatttgaaaa agcagaaaat
     7201 gttatatacg catatgcaaa aagactgtat gatatgtttt aacagaggta ttatatgaat
     7261 aagattataa gaggcaaaga aatactaaca tcaaaagaaa gaattcaaag gacattcgcc
     7321 tttgaggatg ttgacagggt ggcattagga tatgatacaa atcctgtagc acatgagaac
     7381 ctgtgtaaag tattaaatct tgattctaag gacaggaatg ctttcaataa gcatcttgga
     7441 attgaccata tgggagcaag aatcaagtat aatggaccta aaatttatga ggatgtacct
     7501 caaagaacaa ggaactcaga acatggagct ataacaaaat atgtacagaa tcaatttgga
     7561 gcatactggg atttttgtga ttttcctctt gaagatgctg atgatgatgt tatttacaat
     7621 tatccttatc caagtgcgga tgattatgat tacaaagcct gcgctgatta tgtggattat
     7681 cttgttaagg aaggctttgc aattcatgtc ggagatccag gtttagggga tattcttaat
     7741 accactggaa tgataatggg tgttgaacag gctttaatta atatagctac agagcatgag
     7801 cctacaattc acactataga taaaagactg aatttgcagt taaaaagaac agaaaaaata
     7861 ctagaaatga ataaaggtaa gatagatttc atgtggttag gggaggattt aggtactcag
     7921 catactccta tgatatcaat ggaactatat agaaaagttc ttaagcctag gcatcagaag
     7981 tttattgatt tggcaaaaag tttcaatctg ccagttataa tacatacctg tggaagctca
     8041 tcatgggttt atgaagagtt tatcgagatg ggaatgaatg gtgttgatac attacagccg
     8101 gaagccgtca atatgagtcc tgaatatcta gtcgaacatt ttggagggag gcttgttttt
     8161 agaggatgta tttccacagc tggacctttg gcatatggaa cacaaaagga gacagagcga
     8221 gtatgcatag atacgcttga gataatgagt aaatgtaaag gatacatatt tgcacctact
     8281 cattcaatac aggataatac accacctgag aatactataa ctatgtacaa tactgcatat
     8341 tcattcaaaa aaggaggaag caaatgaaat cagtatcaat ttctaacaag acaatgaaga
     8401 tactaattaa aagccagaga gatgaagaaa caggcgcgct ggtttatgag tatctgtcaa
     8461 aaaaagaaaa ggaccctaaa aatagcgaaa ttctcaataa aatgagcaaa gatgaaaaaa
     8521 gtcatgctat gacatggaaa aactatacta aaaagaatgt taaacctgac aagatgaagc
     8581 ttgcttttgt taagtttatt tctattctgc ttggatatac ctttgttctt aagataattc
     8641 agaaagatga aggttttgcg caaaaagaat acgagattat aaagcaggag ataccagaag
     8701 ctttgaagat acaggaagat gagcatactc atgagatgga tcttatcact atgcttgatg
     8761 aagagaggct tcaatatgtt ggtgcgatgg tacttggatt aaatgatgct ttggtggaat
     8821 taaccggagc tatagcaggt ctttcatttg cattagcaaa tacaaagata gtcgcactat
     8881 ctggaattat aactggaatt tcagcaacct tgtcaatggc tgcatctaat tatcttgctg
     8941 aaaaagctga cggtaatccg aatgctttca aatcaagtat ttttacagga gttgcctatc
     9001 ttattacagt agctttaatg gtaatgcctt atcttcttct tccagtaaac gcatatgtag
     9061 aagcattcct tataatgata ggcgttgtta tattgattat attagtgttc aactactata
     9121 tctctgtggc aaaagatcaa cctttcttta aaaattttgc aaagatggca attataagta
     9181 tatcagttgc aggtatttca tatctaatcg gaatacttgc aaaagcattg cttggcattg
     9241 atgtaattta aaagaattac ttaattaaat aatttgtata aaagaaacga gagcttattg
     9301 ctctcgcttt ataatgatta ttatgtgcac ttaactatca atatgctgta tgctgataaa
     9361 aagtatattc atataatatt tattactcag aaaacaaaaa atcttcaatc ttaaatgtat
     9421 atcctcttat ctggccttta ggttcatcgt caagaagata atttaccaca tatatagttc
     9481 cgtcctcaaa ctgtacccag cctgaatatc caagatcagc tttgggattt ctgtcataat
     9541 caatgggaaa tattcttgtc tgctgctgtc ttctttcttt ttctaaagca gtttgcttat
     9601 caaatatgca tcccataaaa ttttgagttg ttctgcccca gccctgtttt ccgccttgta
     9661 taaacctata ggtcataagg attctgccat cggataaaaa gcctgtaaca ggtctgtgac
     9721 agcctggtat gggtatctta tatactccat cccaagtctc acccccgtca tatgaaaagg
     9781 ctttaaaaca gtcatatccc tcccacgaat tctcacgtaa aaaagcaata aggacattat
     9841 cttcgacttc aagtattgat gcctcgcata aattgtattt ttcatctttt gctactgtaa
     9901 ttctgtctga ccaggtaatt ccagaatcat cactgtacca aaggtactga actagtttat
     9961 taatgcttat gtcaagaaaa tgagaagaca gtatccatct tccgcttttt aatactagaa
    10021 gcttgtcagg tactatgcct tgagaagggg tgtcaaaagg gcctttccat gtttttccat
    10081 catcaatgga tttccacaaa tatgtttttg cattatgaga ttcatttttt attacataat
    10141 cagctataat aaccaaagta tcatctggca gttttgaaat tctggcgcag ttgtaatagt
    10201 tatctgatgt gcttttttct gtaagtgcag ttttatcaga ccatgttttg cctttgtcca
    10261 gactttcttt atatactatc cttgcctggt ctctgtctcc gtgatgtttg cattctgaaa
    10321 acacgcatac taatttacca ctcttagtct taactatgtc tggccatgct tcgtatattg
    10381 atttatcatt actgactaca tatttttcta acgccatttt attttccctc tttttctgga
    10441 agttcccaga atgtgccgaa aatctcacac tgagaatcca gagtttcttt ctggtaatat
    10501 actgttaata attttccatc atttaactcg acagaagccg gatatcctaa atcatgaata
    10561 ggtccgtcat ctctcaatat tatctcatca gaccatgtct tgcatctgtc ataagatacc
    10621 atagctcttt gaccatatgg ttgttctctg tatccgtaaa ccaagacaac ggcaccgcag
    10681 ctgtgttcga ttgcatgagc aggtgcgcca tgcttattta tccttacagg tacagtccat
    10741 gtttttcctt tgtcgtatga ttcggtcata tatgttgcaa atatgcctcc ttgaacacga
    10801 ataaatccaa caagtttttc ttcaagttca agtatgtatg gttcgcaatg aagatattca
    10861 ttaccttcat cagtaacata tggtacatga gacagataat cccactttaa accgtctttg
    10921 ctgtaaaaca ctaataactc ctttttgaat cggagttttt catcgtattt tgaaggatta
    10981 tcatcaactt ccttgtaata tcccatgtat ataaggcctc catcttttaa agcggttggg
    11041 ccatgagggc aggtaacggg tgcatgctta acttcagacc atgttatgcc cttatcctcg
    11101 gatattacat atgtagaacc aaaatatttt ttctccattt gctttgttat ctgattattg
    11161 gaaaagtaag catcacgcat tgctaactta aatccttcag cagggaactt gctttgtaca
    11221 tcaataaggt ggttgaaaga tgtcacaatt atcttgtctt tattaaatac agttattccg
    11281 gcatctctgt cgtctagcgg agtatcaatt attatctcag gggcagtcca gttttttcct
    11341 tcatcataac ttctggataa aaggactttg ccaaatgggc agacatggcc tagccggttt
    11401 cctgagcata ctgaaataag ttctccattt aggcctctgc atacagacgg ccatccatga
    11461 taaccgaaaa tcgagtcctt gctcttgaaa atcttaacat gttcggtttt tggtctaatc
    11521 atattaattt gttcctcctg aaaataattt gagtttgatt acattattaa attatagtat
    11581 catatcaggc tcataatcaa gcaaattatg gattaatatt gaaaatttaa ataaaaatgt
    11641 ctggaattat atttttgtat aatatatcaa taatatctgg tgtataattt tttcatagca
    11701 gttagacgct gcaaatacaa ttacgggaga agataatgaa actaacaaat tttatcgaaa
    11761 acggcaaatt ttacaaatct aattttcatg ctcattccac aagaagtgat ggaaaattaa
    11821 gtcaggaaga tgcaataaac gtatttaagg aacacggata taatttctta tgcttatctg
    11881 aacataatat agtcactaaa acggataaat ataatacaga taattttctt ttagtacccg
    11941 gactggaact tcattcattg cttgaacatc cactgacaat acatcacatg cttggactga
    12001 cttctatgga taacgataaa gtttatgacg gaatgaaact atataatata ccttatacaa
    12061 ataatgagga atctttatta gctttggaaa aaacacttac tgatttggac cttcttacag
    12121 tttattgtca tcccacatgg tctcatgtag aaccttatca atatgatttt ctaaaaaata
    12181 ccaatatagt ggaaatatgt aactggggga cagtttttga acaaaatcat aataatgatc
    12241 ctacatactg ggattcctgg cttaagcagg gtaaacagat ttggggaatg gctaatgatg
    12301 attctcataa aagagaacag tactgcggtg gatggaatat ggtaaaatgc gaagaattta
    12361 caattccatc aatgctggac agcttgaaaa aaggtagttt ttattcaaca acaggtccac
    12421 agattcatga cttctatata gaagatggtg ttgcatatgt taaatgttct gaagctaaat
    12481 cgattacatt tattacttta agtaattggg gtaaatgcta tagggatccg gctggaaagc
    12541 ttacagaggc ttctttaaat cttcctttca atgcatatat gacaagagtt gaaattgaag
    12601 acaaagatgg taaaatagcc tggtctaatc caataatgag aatggacagg tttgaacaca
    12661 gataaggagc ttttaaattg aaagtatata aatacagaaa aggttatttg tgtataacaa
    12721 acataagtaa aaatgcaata aggcttagaa caagtcttgt aaatgatttt aatgaaataa
    12781 atccttgtgt cagatatgga atttatgata tggcagaaaa aactgtcgaa gattcagaaa
    12841 tttcaatcct tattgagaat gatcaattat ccattaatgg aatgaaaact gagatacttt
    12901 tcaacaaagg gtttgacttt aaaataaaag ttgcagatga taggttttat ggtcttggtg
    12961 atgctgacag gtccacattg gaaaaaagag gaacaagaca tgttatatgg gtacagaatg
    13021 tgaaatgcta tattccatat ccaatgctgt taagctcgaa tggctggggt attttctctt
    13081 caacgacatt caaacaggtt tttgatatat gttcaaaaga atcaaataca ttatccataa
    13141 gcagcgcaaa aggctcatgc gatatagtta tttttacagg taatggattt gatgagcttc
    13201 tatcacagtt taaaagtatt acaggtcagt ccaagctact cccaagattt gcttacggcc
    13261 tttcatatgt cgcaaatcag aaaattgatg cattcaatat gattaatgaa atgtacactt
    13321 ttcgaaaaga ggatattcca tgtgatgtat atggtcttga acctgagtgg atggagaaaa
    13381 gatatgattc atctgtagat aaaaaattca atactacaaa attttacata ccatactgga
    13441 tggatgtaaa tacaataaga cagaatacct ttttcaaacc catcagtaat atggatttta
    13501 aattaagtct atggttatgc tgtaactatg atctttcata tgaagaagaa agaacagcag
    13561 ttattaaagc tcatgagata agcaaacagg aaactgacaa tgaagatgac ttaagttatc
    13621 ttgatgaaag gtttgaggta gatgatcatc ttggaaatcc tcagtacatg gataagctta
    13681 ctgttaaaga agaaccatgg tttaaacatt tggagaagtt tgtagatatg ggggtaaaag
    13741 cattcaaact ggatggagct tggcaggtag acgaacatcc tgacaggctt tatggaaatg
    13801 gtatgactga tgagcagatg cataatcttt atcctttaat atatgctaag cagatgtctt
    13861 ctggttatga aaatcatact aataccagat ctttagttta ttcatcagga ggttttgcag
    13921 gaatacagca atatgttgca acatggtcag gagatactgg aggaggtccg aaacctttag
    13981 tttctatgct taatcttgca atgtcaggtc atgtgaatac cacttgtgat atgaatgtac
    14041 atagtctgga aggtatacat ttcggattct tgcaaccatg gtcacagcag tgcaactggg
    14101 attattggaa ccagccatgg ctatttacaa aagatttgaa agaagcatat acatactatg
    14161 ataaattaag atattcactg ataccatata tttacagcgc agcatatgag gcatatctta
    14221 atattgctcc tattatgcga cctttagtct atgattatcc tgaagatgaa aacacattta
    14281 atattttaaa ccagtatatg ctgggaaaat atcttctcgt tgcagcattt attgacaaag
    14341 atatttatct tccagaagga atttggatag acttgtttac ccaaaaagag tatcagggaa
    14401 acagatatat ccaatatgtt gtcccacaag gaagaggcgg tgcgctattt gcaaaaaaag
    14461 gcgcaatatt atgtaggtca tatggaatat cgtctatttc tgatgagcct tttgattcat
    14521 acactatcga aatttatcca tctgtgaccg aaaatgaatt tgtgatttat gaagatgatg
    14581 gaatatccct tgaatatatc aatggagcaa gatgcgaaac ttgtataaaa gcatttaaaa
    14641 gagatgatga tataaatata agcatatctg aaagaaaagg ggagtttaca ggtaaaaaag
    14701 ataatgttga atacgagata aatgttatga atgttaataa aagtgatgtt atatacatta
    14761 acggagtatt ctgggaatat aagatagttg gagaagatac tgcagttata aaaattaatg
    14821 aaaaggagtt attttaatga gtgagtataa tgatnnnnnn nnnnnnnnnn nnnnnnnnnn
    14881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14941 nnnnnnnnnn nnnngggcag actgaacatc catttacaat taactttaac agtaatgatg
    15001 ttaggataac tactcattat cacaagaaca atcttctgtc atctatgttt tctgtaatac
    15061 atgaaggagg gcatgctctg tatgaactta actgtgatga cgaatatgat ttcactgctt
    15121 tgtcgggcgg tacatcaatg ggtattcatg aatcacagtc acgatttttt gaaaacataa
    15181 ttggaagaag tagaagtttt acaaattatc tgtatcctta ccttaaaaaa atgtttccag
    15241 ttcagtttaa ggatgttaca aaagtcatgt tatatgaagc attaaacaag tcgcagccat
    15301 ctttaattag aacagaagct gacgagctta catactcttt gcatgtaatg gtcagatatg
    15361 agttggagaa gagattaata aacggttcac tcagtacaaa ggatcttcca gcagaatgga
    15421 acagattata taaggagtat cttggtgtgg atgtgccaaa tgatacaaaa ggagtcctgc
    15481 aggattctca ctggagtgga ggagctatag ggtatttccc atcctattct ttagggagcg
    15541 cttatgcttc tcaaatgtta tttcacatga agaaggatct tgatattgat acacttacac
    15601 aacaagggaa cctgaaacct attgtcgaat ggttaaaaga aagagttcat aaatatgctt
    15661 catcaaagac accagcagaa ataatacaaa tatgctgcaa tgaagatttc aaacctcact
    15721 attacacaga ttatctaaag atgaagttta gttcaatata tgagatttaa gcagttttaa
    15781 ctttttagat atttatatta aataagaatt acagccatta attattggct gtttttcatt
    15841 ttccatgctg tatttaataa ataaactaga gatatgatgc tacttgtact attcataata
    15901 caatttttta tttatgagta tgttaattta tttagatatt attcaccatt tacaaaatat
    15961 tacataaatt acaagttagt gtaaagagtt ttgggaaaaa taatttatta acttaataaa
    16021 ttaagaa
//
LOCUS       DAOSXM010000047        16023 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2111
            NODE_2287_length_16023_cov_11.997871, whole genome shotgun
            sequence.
ACCESSION   DAOSXM010000047 DAOSXM010000000
VERSION     DAOSXM010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864178
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 16023)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16023)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:24:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,261
            CDSs (total)                      :: 2,229
            Genes (coding)                    :: 2,218
            CDSs (with protein)               :: 2,218
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 3 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16023
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2287_length_16023_cov_11.997871"
                     /isolate="AMR_MDS_2111"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            16..699
                     /locus_tag="PLQ68_07005"
     CDS             16..699
                     /locus_tag="PLQ68_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005367881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HXK72438.1"
                     /translation="MEKIYKGKTKDVYKLEDGNYLLKFKDDVTGTDGVFDPGANTVGL
                     TIEGAGKAGLKLTKFFFEKINEQSIPTHYISSDIENQTMTVKPAKVFGKGVEVILRYR
                     AVGSFFRRYGMYCEEGQVLDGYVEVTLKDDDRNDPLITDEALEMLGIMTKSEYKVLVE
                     MTRKIGKIIKEEMLKKNLELYDIKFEFGRVGEDNHVALIDEISGGNMRAYSNGMYVEP
                     LVLERMMLD"
     gene            881..1357
                     /gene="mscL"
                     /locus_tag="PLQ68_07010"
     CDS             881..1357
                     /gene="mscL"
                     /locus_tag="PLQ68_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011016635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HXK72439.1"
                     /translation="MRKRIKKQRIKLWNEFKKFITRGNVMDLAVGVIIGAAFSKIVSS
                     LVNDIIMPLAGLLMGKIDFKSLKWILKLDEEGNVLSSVNYGMFIQYVVDFLIMAACVF
                     LLVKFFAAIKKKAEEIIEKEKKEQEAAAPPAPAAPPVPTKEELLLTEIRDLLRDKK"
     gene            1394..2398
                     /gene="corA"
                     /locus_tag="PLQ68_07015"
     CDS             1394..2398
                     /gene="corA"
                     /locus_tag="PLQ68_07015"
                     /inference="COORDINATES: protein motif:HMM:TIGR00383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HXK72440.1"
                     /translation="MNTSPVQKNDNISKIRITDYCSESIQEYSVNRLNELTSIKDGYI
                     RWIDVDNGVDKELRNQLESIYNIHPVVLSSILEKNKRPKLEDYEDYLFVNATMIHRNN
                     DTLVTETLNIIIKDNYVISIGNMNGDVFDDLRKRIRTLGSHIRKSGTDYLLYSILDSI
                     TDVYFDLLYDLEEKIDKLEEEIIKNSSNDVLAKIRELKNDILSISKSIIPLKESRSLL
                     ISEPSQYITDTTLPYMKDVYNHMTEAIEMVENCREMVVELMELFYTTASNNLNKIIKV
                     LTIISTIFIPLTFIVGLYGMNFKYMPEFGFKWSYPILWVIMLSVTAFMVLYFKKKKWF
                     "
     gene            complement(2691..5288)
                     /gene="clpB"
                     /locus_tag="PLQ68_07020"
     CDS             complement(2691..5288)
                     /gene="clpB"
                     /locus_tag="PLQ68_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008982396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HXK72441.1"
                     /translation="MNIQKFTQKSIEAINQAQSLAIEYQNMQIEQEHLLYALLDQPDS
                     FITQLMVSLNVKPNELISAVMRNIETLPKVSGSGREPDKVYVSKEADQTLNESEKQAK
                     AMKDEYVSVEHIFLALLDTANSRMKELFKSMSIDKNAFLKALASVRGNTKVTSDNPES
                     TYDVLKKYGQDLVELAKNQKLDPVIGRDEEIRNVIRILSRKTKNNPVLIGEPGVGKTA
                     IAEGLALRIVRGDVPDNLKERKIFSLDMGSLVAGAKYRGEFEERLKAVLQEIKKSEGR
                     IIMFIDELHNIVGAGKTEGAMDAGNLLKPMLARGELHCIGATTLNEYRQYIEKDAALE
                     RRFQPVLVDEPTVEDTISILRGLKERYEVFHGVKIQDQALIAAATLSSRYISDRFLPD
                     KAIDLVDEACALIKTEIDSMPSELDAISRKIMQHEIEEAALKKEKDKLSKEHLEKIQQ
                     ELAHMKEDFNSMKAKWENEKNAISKVQKLREEIEQVNAQIEKAEREYDLNKAAELKYG
                     KLPMLQKQLEEQEDIAEKTEENRTLLRDKVSEEEIAMIVARWTGIPVSKLMQSEKEKL
                     LGLEDILHRRVIGQDEAVRKVTESIIRSRAGIQDPNRPIGSFLFLGPTGVGKTELAKA
                     LAQALFDDEKNIIRIDMSEYMEKFSVSRLIGAPPGYVGYEEGGQLTEAVRRKPYSVVL
                     FDEIEKAHPEVFNILLQVLDDGRITDSQGRTVDFKNTIIILTSNLGTQYILEGITEDN
                     EISQDARKQVDMLLKQQFRPEFLNRLDEIVYYKPLSKTEIFTIVDLMIKNLESRLKAK
                     QLSIDISKRAKEFIVDQGFDSIYGARPLKRYIQSHVETLIARMILSEDVEPGTTLNVD
                     YDGSELVIG"
     gene            complement(5305..5970)
                     /locus_tag="PLQ68_07025"
     CDS             complement(5305..5970)
                     /locus_tag="PLQ68_07025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72442.1"
                     /translation="MDKSLYSLILSDEVINKIDTLAYAMRTSRSNYINEVLAGHVSYI
                     TPSQRIKDILEEAKSFLEPEGRYAFVEMSSNSYMDIRSALKYRYRPTIRYCIEMTGQT
                     QGPFCRLKAQVRTQSKDLIIYIERFFMIWQAVEDKLMSDKFESLELASYDNVCYTRSF
                     FLKDMDYIMSESGIGEAIAAYIVTLDNAINAYMDNIGDDKKIDSDIYNIYMDYFNSSD
                     LII"
     gene            6164..7420
                     /gene="uvsE"
                     /locus_tag="PLQ68_07030"
     CDS             6164..7420
                     /gene="uvsE"
                     /locus_tag="PLQ68_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007058972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009650 - UV protection [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UV DNA damage repair endonuclease UvsE"
                     /protein_id="HXK72443.1"
                     /translation="MKIGYACLTVAVADTDMKSCIAKNATNENLKSIISNNLQSLLNM
                     LIYNVKNDIKLLRISSDLIPFGSSSINKLEWQHIFKNEFEQIGEYIRNNELRVSMHPG
                     QYTVLNSVNEQVVQRAVDDLKYHCDILDLMQMDSSHKIILHIGGAYNNKLDAIDRFIK
                     NYTALDTRIKKRLIIENDDKIYNIREVLDISKKTSIPVVYDNLHNYANPYDKNILDKE
                     WITECSSTWKKEDGNQKIHYSQQDNNKKTGSHSPTIHINEFLSFYNHIKECDTDIMLE
                     VKDKNISAIKCINLLSKKKNIKYLEQEWSRYNYSVLEKSHKDYLDIRQMLKDKSAYPV
                     KEFYCKIEDVLDLDESIPSAVNALQHVWGYFKNKAEEKEKKKIQKLMEDYTKGLIPLN
                     KVKSFLYTLTLKYNDSYLIQSYFFHI"
     gene            7434..8453
                     /locus_tag="PLQ68_07035"
     CDS             7434..8453
                     /locus_tag="PLQ68_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009250961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HXK72444.1"
                     /translation="MNRRETVIQALKHKQTDLCPFTVNFTKQAVDNLVNYTKNPDILS
                     KFDTYIQVAEYWGRPTEISEKPNFYLDDFGIIWNRSGADKDIGIPDNRAIKDIENNDY
                     VFPEVDEAYIRNLFDNLVKTKQDRFAVAGLGFSLFERSWTLAGMPQVLMAMAEKSDGL
                     HKLFDDICDFNLKIIDVALEYDIDGFYFGDDWGQQKNMIMGPEYWRDYIKPRLKRMYK
                     RVKDKGLYVIQHSCGDIEPVFDDLVEIGLDCYQTFQPEIYDIEKIKDIYTGKLAFWGA
                     ISTQQLLSRASADEVKKETARIMKILFKNGGYIASSTHAIEFDVPAENILAMYDVFTN
                     QDKYL"
     gene            8603..9085
                     /locus_tag="PLQ68_07040"
     CDS             8603..9085
                     /locus_tag="PLQ68_07040"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HXK72445.1"
                     /translation="MNNLQFAIKMEYDGEKFYRNQAKLNKDNNLNEVCLMLAEDEKNH
                     AVLLENKMKDLNYELPASSIANEKTIFENLKDLSIEEKEKLSQLDFYILAADMEKQSI
                     KLYRKYLDASPGKRSREIFEYLIKQEQHHFELIDSLAEALRHAEEWIENAEFGNRPEY
                     "
     gene            9102..10283
                     /locus_tag="PLQ68_07045"
     CDS             9102..10283
                     /locus_tag="PLQ68_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013360968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HXK72446.1"
                     /translation="MKKLVKNNVYWVGKTDWELKRFHGDEYSTHHGSSYNSYLILEEK
                     TVLIDTVWAPFDKEFVENLESEIDLSSIDYIVTNHGEVDHSGSLCELMKKIPNTPIYC
                     SANAVKSLKGQYHQDWDLRVLKTGDTLDVGNGKKLVFVEMRMIHWPDSMAAYLTKDNI
                     LFSNDAFGQHYASDHLFNDLSDQCILYSEAEKYYANIITPFSAVLAKKLEEITAMNLE
                     IDIIAPSHGTIWRDNPMQIVEKYSKWCKDYQENQITIIYDTMWNGTKMLAEKIAQGIR
                     DTDSEVKIKIFNLPKSDVNDITTEIFKSKMVLLGSPTINNCALHSLAALTHYMKGLKF
                     KNKKASAFGCYGWSGESVKILNEMLADAGFDVVNDGLKCLWNPDDEVLKKAYDFGKEI
                     A"
     gene            10280..11245
                     /gene="msrA"
                     /locus_tag="PLQ68_07050"
     CDS             10280..11245
                     /gene="msrA"
                     /locus_tag="PLQ68_07050"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HXK72447.1"
                     /translation="MSTIIKDIYLAGGCFWGTQKYFSLIKGVKNTQVGYANGNTANPS
                     YEQVCHNNTGHAETVKIQYNPKEASLDFILDMFYDVIDPTSVNRQGNDRGTQYRSGIY
                     YVDEADLKIISESIKKLQSKYDKPIAIEIKPLLNYYPAEKYHQDYLDKNPNGYCHIHP
                     QKFIKAEKAIDVLYEKKDKEYLKKTLTPLQYEVTQNNATEPPFKNEYFDNKKEGIYVD
                     ITTNEPLFVSSDKFDSGCGWPSFTKPIEQKSVKQKTDFTHGMVRTEIRSSIGDAHLGH
                     VFTDGPVDLGGLRYCINSASLKFISKEDMESLGYKDYLYLFDKKE"
     gene            11248..12225
                     /locus_tag="PLQ68_07055"
     CDS             11248..12225
                     /locus_tag="PLQ68_07055"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HXK72448.1"
                     /translation="MVKTKKTIEQLEKELEEALALNAQLIEEKQQDMNLNYAWTGNLG
                     HWYWHVPSNKVTFNPLKITVLGYDENEIPKEVTYQYFTDKLHPDDYEKAMQIMRNHLQ
                     GKLSVYEVEYRIKAKDGTYRWYYDRGKITKYDKNNKPELIAGIVFDITEKKMLEEKLE
                     QQNKQLYELSLTDGLTKVRNHRALIQILEEEIRKSDESDKPLSIALFDLDFFKKVNDT
                     KGHLCGDEVLVDLGRIMMQNVRSSDTVGRYGGEEFMMIFPGTSLDYAKEICEKIRLAV
                     EEFKFSCDLKVTISAGVCEYDKTGISDAIRRVDTLLYKAKNNGRNRIEA"
     gene            12339..12761
                     /locus_tag="PLQ68_07060"
     CDS             12339..12761
                     /locus_tag="PLQ68_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009145806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HXK72449.1"
                     /translation="MKISTKGRYALRIMIELAQNKNDKPISLKQISQKQDISAKYLES
                     IISILNKHNFVFSTRGINGGYVLSKEPSEYTIGSILRVTEGSLSPVNCLECQPNRCIR
                     SADCLTLPLWQNIGKMINDYLDSITLEDILNKNINKSI"
     gene            12807..13577
                     /gene="nagB"
                     /locus_tag="PLQ68_07065"
     CDS             12807..13577
                     /gene="nagB"
                     /locus_tag="PLQ68_07065"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006046 - N-acetylglucosamine catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HXK72450.1"
                     /translation="MKIIVKDNYEDLSVFAASIVADLVKRKPNCVIGFATGSTPIGMY
                     HELARLHKEEGLDFSQVTSFNLDEYLGISIDLEKPYEMDQSYARFMYEELFSRINIKQ
                     ENINVPYGLAENPDQFCRQYEEKIKSLGGIDMQILGIGGDGHWGFNEPGSSFDSRTRV
                     EKLNPKTIDDNYEAFYKKAGVSKEEMPRYALTMGIGTILETKSALMLVNGEKKADIIQ
                     KALEGPLSTEVTASAIQIYKGDATVILDKAAASKLKNI"
     gene            13660..14835
                     /locus_tag="PLQ68_07070"
     CDS             13660..14835
                     /locus_tag="PLQ68_07070"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HXK72451.1"
                     /translation="MTKFLIRLFIENHENKSDPQVRSAYGMLAGIVGVSINILLFICK
                     AIIGFLSKSIAILADSFNNLSDAGSFFVSILASKLSKKPADKEHPFGHQRIEYIAALV
                     ISLIIMYAGLTLFIDSIKRIFNPQVLVFDWIFIIILAATMLFKVWLMLFNKNIGEMIN
                     SNVQKAAYIDSRNDLIVTTLTIISMLIAKYFSVSLDGFFGIAVSVFVFISGLNIAKTA
                     LLPLLGAAADKKLYEKITDKVEAYAGILGTHDLIVHNYGPSNIMATIHAQVPSDSDLQ
                     KIHDTIDKIERDVYKEFGISLVIHMDPQQINDVRYFIYKSMVDSIVDHIDMDASIHDF
                     HFQTRNKNILLKFEVCVPHSYTEQQENEIVKTIKRKISETNPHIKCDITVEHAFIES"
     gene            15001..15969
                     /locus_tag="PLQ68_07075"
     CDS             15001..15969
                     /locus_tag="PLQ68_07075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72452.1"
                     /translation="MKRKLFYILLTCEAALCIIFCFVKLSWPMLFSSIMAFPFEQIAH
                     VLRMLSLSSPTGNVIAFVLYFLISLIPLMFLLIFSKNHKLKAEDILLLVLTGILFAAI
                     YFMINPATLTNMLGSTLSVSVIKAFLGVIIYSIIAGYVILRALRMFSNTDKSNLYKYL
                     KILLAILNVIFIFLIFGTLFNNMIYSFNSFTANNNMPGQNLWLNYVFILFNYIISALP
                     YILNIFIVIKVLNLLDNLNEGSYSEKSVTLADSLSKWCIKALSITVVSNIVFNVLQLV
                     LIKKLLVIESTVQIPLFSIVFVLATLLMAKYINENRKLKSDNDLFI"
BASE COUNT         5639 a         2593 c         2751 g         5040 t
ORIGIN      
        1 ttttgaggta tagcaatgga aaaaatctac aaaggtaaaa caaaagacgt atataagctg
       61 gaagatggaa attatctttt aaagtttaaa gatgatgtaa caggtactga tggtgtattt
      121 gatcctggtg caaatacagt cggattgaca atagaaggtg ctggaaaagc tggtctaaaa
      181 ttaaccaagt tctttttcga aaagataaat gagcagtcta taccgacaca ctatatctct
      241 tcagatattg agaatcagac catgacagta aaacccgcaa aagtatttgg aaaaggcgtt
      301 gaggttatat taagatatcg tgctgtagga agctttttca gacgttatgg aatgtactgt
      361 gaagaaggac aggttttaga cggatatgtc gaggtaacct tgaaggatga tgacagaaat
      421 gatccactta taactgatga agcacttgaa atgcttggga taatgaccaa atcagagtac
      481 aaggtgcttg tagagatgac aagaaaaata ggtaagatta taaaagaaga gatgctaaag
      541 aagaaccttg aattgtatga tattaaattt gaattcggaa gagtcggaga agataatcat
      601 gtagcattaa ttgatgaaat ttccggagga aacatgagag catacagtaa tggtatgtat
      661 gttgaaccgc ttgtacttga aagaatgatg ctggattaaa tgataattaa ataaatactt
      721 gtggtgacct gtcagtaatg gcaggttttc tttatgttac gaaaaacaat ataaaaaaat
      781 atttagcaag gaatgtatat tttatgtttt tcatttgaca ttgaatgctt cgacttatat
      841 catttcattt aaagaacaat atgtttatta ggaggaactt atgcgtaaac gtataaaaaa
      901 acaaagaata aaactttgga acgaattcaa aaaattcata accaggggca atgttatgga
      961 tcttgcagtc ggtgtaataa tcggagcagc tttctctaaa atagtatcat ctttagttaa
     1021 tgatataata atgcccttag caggtctttt gatgggaaaa attgatttca agtcattaaa
     1081 atggatattg aaactagatg aagaaggcaa tgttctttcg tcagtaaatt acggaatgtt
     1141 tatccagtat gttgttgatt ttttgattat ggctgcatgc gttttccttt tggttaaatt
     1201 ctttgccgca ataaagaaaa aagcagagga aattatagaa aaggaaaaga aagagcaaga
     1261 agcagcagca cctcctgcac ctgctgcacc tcctgttcca actaaggaag agttgttgct
     1321 tacagaaata agggaccttt taagagataa gaaatagtcc gaacaaataa gtacaagtaa
     1381 aggatgaaaa aagatgaata caagtcctgt acaaaaaaat gataatattt caaaaataag
     1441 aataactgat tactgcagtg aaagtattca agaatattca gttaatcgac ttaatgaact
     1501 taccagcata aaagatggat atatcagatg gattgatgtc gacaatggtg ttgataagga
     1561 attaagaaat caacttgaaa gtatatacaa tattcatcct gtagtcttat caagcatttt
     1621 agaaaaaaac aaaagaccta agctggaaga ttacgaagac tatttgtttg tcaatgcgac
     1681 aatgatacat cgcaacaatg atactcttgt aacagaaaca ttaaatataa ttataaaaga
     1741 taattatgtc atttcaattg gaaatatgaa tggtgatgta tttgatgatt taagaaaacg
     1801 catcagaact cttggaagcc acatacgaaa atctggtacg gactatcttc tttattcaat
     1861 acttgactct attactgatg tttattttga cttgctatat gacctagagg aaaaaattga
     1921 taaacttgaa gaagaaataa ttaaaaactc cagtaatgat gttcttgcaa aaatcagaga
     1981 actaaaaaat gatatccttt caatatcaaa atcaataatt ccattaaaag aatctcgttc
     2041 tcttttaata agtgaaccat cacaatatat aacagatacc acattgccat atatgaaaga
     2101 tgtgtataac cacatgacag aagcaattga aatggttgaa aattgtcgtg aaatggttgt
     2161 cgaacttatg gaattatttt atacaaccgc aagcaataac ttaaacaaga taataaaagt
     2221 acttacaatt atctcaacaa ttttcatacc cttaaccttt atagtcggac tttacggaat
     2281 gaattttaaa tatatgccag aatttggttt taagtggtct taccctattt tatgggttat
     2341 tatgctttca gttacagctt ttatggtttt atatttcaaa aaaaagaagt ggttctgatt
     2401 atctgttgtt aatcagtctt tgaatattta tatttactaa ttattgatac aaaaaggagc
     2461 cagttccggt cagaccggac tggctcctat aaattgcccg ccgatgacaa ttaagcataa
     2521 tgcgacttat ttttaaagga ggtagcgggc cacattttgc catctcctta cgtgttggcc
     2581 tcacgaggag tagcctgata ccattatact cttattgata tattatatca acaaggatat
     2641 agaaaattta tcattatcct gtaaggttag tacttaaacc ttttgttttc tcatcctata
     2701 accagctcac tgccatcata atctacattc aaagttgttc ccggttcaac atcctctgat
     2761 aaaatcatcc ttgcaatcag tgtttcaaca tggctttgaa tatatctttt tagagggcga
     2821 gctccatata tactgtcaaa gccttgatcc actataaact ctttagctct ttttgatata
     2881 tctatagaaa gctgttttgc tttaagtctt gattcaagat ttttaatcat aagatctact
     2941 atcgtaaata tttcagtttt tgataaaggt ttgtaatata ctatttcatc cagtctgttt
     3001 aaaaattccg gtctgaactg ttgttttaag agcatatcaa cctgttttct tgcatcttgt
     3061 gaaatctcat tgtcctctgt tattccttca agtatgtact gtgttccaag attagatgta
     3121 agtatgataa tagtgttttt aaaatcaaca gtccggcctt gcgagtcggt tatccttcca
     3181 tcatccagta cctgaagaag tatattaaat acctctgggt gagctttttc tatctcgtca
     3241 aataagacta cagaataagg ttttcttctt actgcttctg taagctggcc tccttcttca
     3301 tatcctacat atcctggagg tgcgcctata agcctgctga ctgagaactt ctccatatac
     3361 tcactcatat caatcctaat aatgttcttt tcatcatcaa acaaagcctg tgcaagagct
     3421 ttagcaagtt ctgtttttcc aacacctgtc ggacctagga acaagaaaga acctataggc
     3481 ctgttagggt cttgtattcc ggcacgggaa cgaattatag attcagtaac tttcctgact
     3541 gcttcatcct gtcctattac tcttctatgc aaaatatctt caagccccaa taatttttcc
     3601 ttttcgcttt gcataagttt tgaaacagga attccagtcc accttgcaac tatcattgct
     3661 atctcttctt ctgacacttt gtctcttaac agcgtcctgt tttcttctgt cttttcagca
     3721 atatcttcct gttcttctaa ctgtttttga agcataggta attttccata ctttagttca
     3781 gcagctttat ttaaatcata ttctctttct gctttttcaa tctgcgcatt aacctgctct
     3841 atctcttctc gcaacttctg aacttttgat atcgcattct tctcattttc ccattttgct
     3901 ttcatggaat tgaagtcttc tttcatatgt gcaagttcct gctgtatctt ttcaagatgt
     3961 tcttttgaca gtttgtcttt ttctttcttt aacgctgctt cttctatctc atgctgcatt
     4021 attttcctcg atatcgcatc aagttcagaa ggcatagagt caatctctgt ttttattaat
     4081 gcacatgctt catcaacaag atcaattgcc ttatcaggca agaatctgtc agatatatat
     4141 ctgcttgata aggtagcagc cgctataagt gcttggtcct gtatttttac tccatgaaat
     4201 acttcatatc tttcctttaa tcctcttaat atagaaatgg tatcctctac tgtcggctca
     4261 tcaaccagta ccggttggaa tcttctttcc agagcagcat ctttttctat gtattggcga
     4321 tattcattca aagtagtagc acctatacag tgcaactccc ctcttgcaag catgggttta
     4381 agaagattgc ctgcatccat cgctccttct gtcttgccag ctccgacaat attatgaagc
     4441 tcatcaataa acatgattat tcttccttca gatttcttta tttcctgaag tacagccttt
     4501 agtctttctt caaattcccc tctgtatttt gctcctgcta cgagtgatcc catatcaagg
     4561 gagaatattt ttctttcttt tagattatcc ggcacatcac ctcttactat tctaagtgca
     4621 agaccttcag ctattgcagt tttaccgaca ccaggctccc caataagcac tggattattt
     4681 ttggtctttc ttgatagaat tcttattaca ttacgaatct cttcatccct tccgataaca
     4741 gggtctaatt tctgattctt tgcaagttcc acaagatcct gtccatactt tttcaataca
     4801 tcataagtcg attcaggatt gtctgatgta acctttgtat ttcccctaac tgaagctaaa
     4861 gcctttaaaa aagcattttt atcaatactc atactcttaa aaagttcctt cattctggaa
     4921 tttgcagtat ccagaagagc aagaaaaata tgctcaaccg aaacatactc atccttcatg
     4981 gcttttgcct gcttttctga ttcattcaaa gtttggtcag cctctttaga tacataaacc
     5041 ttatccggtt cgcgaccgct tccagaaact ttcggtaatg tttcaatatt tctcattaca
     5101 gcagatatga gttcattcgg tttaacatta agagaaacca taagctgagt gataaaacta
     5161 tcaggctggt caagtaatgc ataaagcaga tgctcctgtt caatctgcat attctgatac
     5221 tctattgcta aactctgagc ctgatttatc gcttctattg atttttgtgt aaatttctgt
     5281 atgttcatac tacacctccg taattcaaat aatcaaatct gatgaattaa agtaatccat
     5341 ataaatattg taaatatcag agtctatctt tttatcatct cctatgttat ccatgtatgc
     5401 atttatagca ttatccagtg taacaatgta tgctgcgata gcctctccaa tcccgctttc
     5461 gctcataata taatccatgt ctttaagaaa aaaactccgt gtataacaaa cattatcata
     5521 tgaggccagt tccaagcttt caaatttatc tgacatcagt ttatcctcaa ctgcttgcca
     5581 aatcatgaaa aacctttcaa tataaattat caaatcttta ctctgagtcc taacctgtgc
     5641 tttaagcctg caaaatggtc cctgagtctg tccggtcatc tctatgcaat aacgaatagt
     5701 gggacggtat cggtacttta aagcactcct tatatccata tatgaatttg aactcatttc
     5761 tacaaaagca tatcttcctt caggttccaa aaaagattta gcttcctcta gaatatcttt
     5821 tattctttgt gaaggagtta tatatgatac atgacctgcc agtacttcat ttatgtaatt
     5881 agacctgctg gttctcattg catatgcaag tgtatctatc ttattaatga cctcatcact
     5941 aagtatcaaa ctatataaag atttgtccat ctagtaccgc ctttcataat aattgtatca
     6001 ctaaatatgt aacttgacaa cgatattata taactgtgtt tacaaattta actaagtatt
     6061 agtaaatata ataactttac tttgttttat tctttatgca tttatctgta tatgtagatt
     6121 aaaaagaaaa gataatataa aataacatta tatggaggac aagatgaaaa ttggatatgc
     6181 atgcctgact gttgcagttg cagatactga tatgaaaagc tgtattgcaa aaaatgctac
     6241 aaatgagaat ttaaaatcaa taatatcaaa taatctgcaa tcattactca atatgcttat
     6301 atataatgtt aagaatgata ttaaactgct aagaataagt tcagacctta taccctttgg
     6361 ctcaagcagt ataaacaaat tagaatggca gcatattttc aaaaatgaat ttgaacaaat
     6421 tggagaatat ataagaaata acgaactaag agtatcaatg catcctggtc agtatactgt
     6481 tctgaattct gttaatgagc aggttgtcca aagagcagtt gatgatttga aatatcattg
     6541 cgatatactg gatttgatgc aaatggacag ttcccataaa ataatacttc acatcggagg
     6601 cgcatataac aataagcttg atgctattga taggttcatt aagaattata ctgctcttga
     6661 tacacgaatt aaaaagaggc ttatcataga aaatgacgat aagatatata acattagaga
     6721 agttttagat atatccaaga aaacttcaat tccggtcgtt tatgataatc tgcacaatta
     6781 tgctaatcca tatgataaaa atatcttaga caaagagtgg ataactgaat gttcttcaac
     6841 atggaaaaaa gaagatggaa atcagaaaat tcactattct cagcaggata ataacaaaaa
     6901 aaccggttcc cattccccta ctatccatat taatgaattc ttgtcttttt ataatcacat
     6961 taaagaatgt gatacagata ttatgcttga ggttaaagac aaaaacatat ctgcaataaa
     7021 gtgtatcaat cttttatcca aaaagaaaaa cattaaatat ttagagcagg agtggtccag
     7081 atacaattat tcagtactgg aaaaatcaca taaggattat ttagatataa ggcaaatgct
     7141 taaagacaaa tcagcttatc ctgttaagga attctactgc aagatagaag atgtgctaga
     7201 tttagatgaa tcaatacctt ctgctgttaa tgctttacag catgtatggg gatattttaa
     7261 gaataaagca gaggaaaaag aaaaaaagaa aatacagaaa cttatggaag actatactaa
     7321 aggtcttatc cctcttaata aagtcaagtc atttctatat acactaactc tcaaatataa
     7381 cgatagctat cttattcaat catacttttt tcacatttaa aaggagcaga acaatgaaca
     7441 gacgagaaac agtaatacaa gcactaaagc acaaacagac tgatttatgc ccctttactg
     7501 tcaacttcac caagcaggct gttgacaatc ttgtaaatta tacaaaaaac cctgacatat
     7561 tatcaaaatt tgatacatat attcaggttg ctgagtactg gggacgccca acagaaattt
     7621 ctgaaaaacc gaatttctac cttgatgatt tcggcataat atggaatcgg tcgggagcag
     7681 acaaggatat aggtattcct gataatcgtg caattaagga tattgaaaat aacgattatg
     7741 tttttcctga agttgacgaa gcttatataa gaaatttgtt tgacaatctt gttaaaacaa
     7801 aacaagacag atttgcagta gcaggtttag gattctcttt atttgaaaga tcatggacgc
     7861 ttgcaggtat gcctcaggtt ctaatggcta tggctgagaa atcagatggt ttacataagc
     7921 tttttgatga tatatgtgat ttcaatctca aaataattga tgtcgcctta gagtatgata
     7981 tagatggctt ttatttcggt gatgactggg gtcaacagaa gaatatgata atggggcctg
     8041 aatattggag agactacata aaaccaagat tgaaaagaat gtacaaaaga gttaaagaca
     8101 aagggcttta tgtaattcag cattcctgtg gtgatataga accggttttt gatgaccttg
     8161 ttgaaatagg tttagattgt tatcagactt ttcaaccaga aatttatgac atagagaaga
     8221 taaaagacat atatacaggt aaacttgcat tctggggtgc aatatcaact cagcagcttc
     8281 tatcaagagc ttcagctgat gaggtaaaaa aggaaacagc caggattatg aagatattgt
     8341 tcaaaaatgg tggatatatt gcgtcgtcta cacatgctat cgaatttgat gttcctgctg
     8401 aaaacatttt agcaatgtat gatgtcttta ccaatcagga taaatattta taaattctaa
     8461 ttagtactag attttaatat taatacaaat aaaataacat atatatggta taatttaaac
     8521 atatattgtt atttgaaaag taatattcta gattagacta aatctagatt aaaagtactc
     8581 attacaacag aaggaggata aaatgaataa tctgcagttt gctataaaaa tggaatatga
     8641 cggtgaaaaa ttttatagaa atcaggctaa gttaaataag gataataact tgaatgaggt
     8701 ttgccttatg cttgcagaag atgagaaaaa tcatgccgtt ttattggaaa acaagatgaa
     8761 agatttaaat tatgagttgc ctgcttcatc cattgcaaac gaaaaaacca tttttgagaa
     8821 tcttaaagac ctttcaattg aagaaaaaga aaaactcagc cagctggatt tttatatcct
     8881 tgccgctgat atggaaaagc aaagcataaa actatacaga aagtatcttg atgcatcacc
     8941 tggcaaaaga agccgtgaga tattcgaata tcttataaag caagaacagc atcattttga
     9001 actcattgac tctcttgcag aagctttaag acatgctgaa gaatggatcg aaaatgctga
     9061 attcggcaac agacctgaat attgataaga aaggaagaat tatgaaaaag ttagtaaaga
     9121 ataatgttta ctgggttgga aaaacagact gggaattgaa aagattccat ggagatgagt
     9181 attctactca tcatggttca agttataaca gctacttgat tcttgaggaa aaaacagttc
     9241 tcatcgatac agtctgggcc ccctttgata aagaatttgt agaaaatctg gaaagtgaga
     9301 ttgatctttc ctccatcgac tatatagtaa caaatcatgg tgaagttgac catagcgggt
     9361 cattatgtga gttaatgaag aaaataccta atacccctat atactgcagt gcaaatgcgg
     9421 ttaaatcttt aaaaggtcaa tatcatcagg actgggattt aagagtgttg aagactggag
     9481 atacccttga tgtgggtaat ggtaagaaac tggtttttgt cgagatgcgc atgattcact
     9541 ggcctgacag tatggctgca tatcttacca aagataatat attattcagc aatgatgcat
     9601 tcggacagca ctatgcctca gaccatctgt ttaatgacct ttcagaccaa tgcatactgt
     9661 attcagaagc agaaaaatat tatgcaaata taataacccc atttagcgct gtgttagcaa
     9721 aaaaacttga agaaataaca gccatgaatc ttgaaataga cataattgct ccaagtcatg
     9781 gaacaatctg gagagataac cctatgcaga tagtagaaaa atattctaaa tggtgtaagg
     9841 attatcagga aaatcagata accataatct atgatactat gtggaatgga acaaaaatgc
     9901 ttgctgagaa aatagctcaa ggaatacgtg atactgattc tgaagtaaaa ataaaaatat
     9961 tcaatctgcc aaaaagtgat gtgaatgata taactacaga aatattcaag tccaaaatgg
    10021 tactgttagg ctcaccgaca attaataact gcgcactgca ttcattagca gcacttacac
    10081 attatatgaa gggcttaaaa ttcaaaaata agaaggcttc tgctttcgga tgttacggat
    10141 ggagcggtga atcagttaag atattaaatg aaatgcttgc agatgcagga tttgatgttg
    10201 ttaatgatgg tttgaaatgt ttgtggaatc cagatgacga agtattgaaa aaggcatatg
    10261 attttggaaa ggaaattgca tgagcactat tataaaagat atatatcttg ctggcggttg
    10321 tttctgggga actcaaaaat acttttcctt aattaaagga gttaaaaata cacaagtagg
    10381 atatgcaaac ggtaatactg ccaatccgtc atatgaacag gtgtgccata ataataccgg
    10441 acatgctgaa acagtaaaaa tccaatataa tccaaaagaa gcatctttag attttattct
    10501 ggatatgttt tatgatgtaa ttgaccctac atctgtaaac aggcagggta acgatagagg
    10561 cactcagtac agaagcggga tatattatgt ggatgaagct gatttaaaaa taatatcaga
    10621 atctataaaa aagcttcagt ccaagtatga taagcctata gcaattgaaa taaagccttt
    10681 gctaaactat tatccagcag agaaatatca tcaggattat cttgataaga acccaaacgg
    10741 atattgtcat attcatccgc aaaaattcat aaaagcagaa aaagcaattg atgtgctata
    10801 tgagaaaaaa gacaaggaat atctaaaaaa aactcttact cctttacaat atgaagttac
    10861 tcagaacaat gcaacagaac ctcctttcaa gaacgagtat ttcgataata aaaaggaagg
    10921 tatttatgtt gatattacta ctaatgaacc cttatttgta tcatcagata aatttgactc
    10981 cggttgcgga tggccaagtt ttacaaaacc catagagcaa aaatctgtta aacagaaaac
    11041 tgacttcact catggtatgg taagaactga gataagaagt tctattggag atgctcatct
    11101 tggtcatgtt ttcactgatg gccccgtcga cttgggaggc ttaaggtatt gcattaacag
    11161 tgcgtcatta aagtttatat caaaagaaga tatggaaagt ttgggatata aagattatct
    11221 atatcttttt gataaaaagg agtaaaaatg gttaagacaa agaaaacgat agaacagtta
    11281 gaaaaagagc ttgaagaggc attagcttta aatgcgcagt taatagaaga aaaacagcag
    11341 gacatgaatt taaattatgc ctggacaggt aatctcggtc actggtactg gcatgtcccc
    11401 tctaataagg taacatttaa tccgttaaag attacagttt taggatatga tgaaaacgaa
    11461 ataccaaaag aggtaacata tcagtatttc accgacaaac ttcaccctga tgattatgaa
    11521 aaagctatgc agataatgag aaatcatctt cagggcaagt tatctgttta tgaagttgag
    11581 tatagaatca aggctaaaga cggtacatac aggtggtatt atgaccgagg aaaaataaca
    11641 aagtatgata aaaacaataa accggagctt atagcaggaa tagtatttga tatcactgaa
    11701 aagaaaatgc ttgaagaaaa acttgaacaa cagaataaac agctttatga gttatcattg
    11761 actgacgggc ttacaaaagt cagaaatcat cgcgcactga tacagatatt agaagaagaa
    11821 ataagaaaat cagatgaatc agataagcct ttgagtatcg cactatttga ccttgatttt
    11881 ttcaaaaaag taaatgatac aaaaggacat ctatgcggag atgaggtact ggttgactta
    11941 ggcagaatta tgatgcaaaa tgttcgttca tctgatactg taggaagata tggcggagaa
    12001 gaattcatga tgatctttcc tggcaccagt cttgattatg ctaaagaaat ctgtgaaaaa
    12061 attcgattag cagttgaaga attcaagttt tcatgtgact taaaagtaac aataagcgca
    12121 ggtgtttgtg aatatgacaa gactgggatt tcagatgcaa taagaagggt tgacacactc
    12181 ttgtataagg caaagaataa tggacgtaac cgaattgaag cttagtattc attccttttt
    12241 tgttgtttga actatattaa tcagatatat agttattaag actatttagc ttaatcccta
    12301 taatcctatg gtataatagg gattaatgga gtaaagcaat gaaaatatcc accaaaggaa
    12361 gatatgcgtt aagaataatg attgaattag cgcaaaacaa gaatgataaa ccaatatctc
    12421 ttaagcagat atctcagaaa caggatattt ctgctaaata cttagaatct ataataagta
    12481 ttctaaataa acataacttt gttttcagca ctagaggaat taatggaggt tatgtattat
    12541 ccaaagaacc ctcagaatat acaataggtt ctatactaag agtaactgag ggtagtttat
    12601 ctcctgtaaa ctgtttggaa tgtcagccta acagatgcat tagatctgct gactgtctga
    12661 ctcttccctt gtggcagaat ataggtaaaa tgattaatga ttatctggat tcaataacat
    12721 tagaggatat tttaaataaa aatattaaca aaagcatata ggtaggtata taatataaga
    12781 aatatcaaca ggaaagaagg acacccatga agataattgt aaaagataat tacgaggatt
    12841 taagcgtctt cgctgcttct atagtagctg atcttgtaaa aagaaaacca aattgtgtta
    12901 ttggttttgc aactggctcc acaccaatag gaatgtacca cgaacttgcc agattacata
    12961 aagaggaagg attggacttt tcacaagtca catcttttaa ccttgatgaa tatttaggta
    13021 taagtatcga tttggaaaaa ccttatgaaa tggatcagtc ttatgcaaga ttcatgtatg
    13081 aggaactatt ttcaaggatt aacatcaaac aggaaaacat taatgttcca tatggcctgg
    13141 cagaaaatcc agatcaattc tgtagacagt atgaagaaaa aataaaaagc cttggtggaa
    13201 tagacatgca gattcttggt ataggtggag atgggcattg gggttttaac gaaccaggat
    13261 catcttttga ttcacgtaca agagtagaaa aacttaatcc aaaaacaata gatgataatt
    13321 atgaagcatt ctataaaaag gcaggagtat ctaaagaaga aatgccaaga tatgctctta
    13381 caatgggtat tggaacaata ttagaaacaa aaagtgcgct tatgctggta aatggagaaa
    13441 aaaaagctga tataattcaa aaagcccttg aaggaccatt aagtactgaa gttacagctt
    13501 ctgcaataca gatttataaa ggagatgcta ctgtaatact tgataaagca gcagcttcaa
    13561 aacttaaaaa catatgattt tatttacata tgacaatagc gcatttatga ttcttttaca
    13621 tcatagatgc gtttttactc agataggaaa acgcaaagta tgacaaaatt tttaataagg
    13681 ctatttattg aaaatcatga aaacaaatca gatccgcaag tacgtagcgc atatggaatg
    13741 cttgcaggaa tagtcggtgt gtccataaat atattattat ttatctgcaa ggcaattata
    13801 ggttttttat caaaaagcat agctatattg gctgattcat ttaacaacct ttcagatgca
    13861 ggctcatttt tcgtaagcat tttagcatct aaattatcta aaaaaccagc tgacaaagaa
    13921 catccctttg gacaccagag aattgaatat atagcggctc tcgtaatttc actgattatc
    13981 atgtatgccg gattaactct ttttatagat tccattaaga gaatcttcaa tccccaggtt
    14041 ttggtttttg actggatatt cataatcata ttggctgcta caatgctctt taaggtatgg
    14101 ttgatgcttt tcaacaagaa tattggagag atgattaact caaatgtaca aaaagcagca
    14161 tacatagatt ccagaaatga tctgattgtt acaaccctga caataatatc tatgcttata
    14221 gcaaaatatt tttcagtatc actggacgga ttttttggaa tagcagtctc tgtattcgtc
    14281 tttatatccg gacttaacat tgcaaaaaca gcacttctcc cccttttagg agcagcagca
    14341 gataaaaaac tatatgaaaa gataacagac aaagtagaag catacgcagg aatacttgga
    14401 actcatgatt taatagtcca caactatgga ccttcaaata taatggcgac tattcatgca
    14461 caggtgcctt ctgactcaga cttacagaaa attcatgata ctattgataa gattgaaaga
    14521 gatgtatata aagagtttgg catcagtttg gtaatacaca tggatcctca gcagattaat
    14581 gatgtacgtt acttcatata taaatcgatg gttgattcca tagttgatca cattgatatg
    14641 gatgcgtcaa tacacgattt ccattttcaa accaggaata agaatatact tctgaagttt
    14701 gaagtctgcg tcccccattc atatacagaa cagcaggaaa atgaaattgt taaaacaata
    14761 aaaaggaaaa tatccgagac caaccctcat ataaagtgtg atataactgt tgagcatgct
    14821 tttatcgaat catagcaact tatatagaag caataaataa acaaatttca aatttataaa
    14881 taatactttc aaacaacaaa gaaaacccca aataaggtat ttaaattaat tattgacaaa
    14941 cgataatttt gcgcatataa tacacatgca attatcgttt tacaataatt tggaggaacc
    15001 atgaaacgaa aactttttta tatcttactc acctgcgagg ctgcattatg cataatattc
    15061 tgttttgtaa agctatcctg gccaatgctt ttcagttcaa taatggcttt cccttttgag
    15121 cagatcgctc atgttttacg aatgctttca ttatcatcac cgacaggtaa tgttattgct
    15181 ttcgtactat attttctaat aagccttatc cctttaatgt ttcttctcat attcagcaaa
    15241 aaccacaaac ttaaagcaga agatatttta cttttagtac ttacaggtat actctttgca
    15301 gccatatact tcatgataaa tcctgcaaca ttgacaaata tgttggggag cactctttct
    15361 gtatcagtga ttaaagcatt tttaggagta ataatatatt ctataatagc aggttacgtt
    15421 atactcagag cactaagaat gttcagtaat acagataaat ccaacttata taaatacttg
    15481 aaaatactat tagcaatact taatgtaata tttatatttc tcattttcgg gactttgttt
    15541 aataacatga tatattcttt taactcattt actgccaaca ataatatgcc tggtcagaat
    15601 ctgtggttaa actatgtatt cattttattt aattatatta tcagtgcatt accctacata
    15661 cttaatatat ttatagtcat taaagtcctg aatcttcttg ataatctaaa tgaagggagc
    15721 tactctgaaa aatcagttac cttggcagac agtctgtcaa agtggtgcat aaaagcatta
    15781 tcaataactg tagtttcaaa tattgttttt aatgtattac agctggtact gattaaaaaa
    15841 ctgcttgtta tagaaagcac ggttcagatt cccctattct ctatagtctt cgtattggca
    15901 acactcttaa tggctaaata tataaatgaa aacaggaaac ttaagagtga taatgatttg
    15961 tttatataaa gaccagctat aaaatgtcaa gcgacatttt aagataaaaa acctaaaagc
    16021 agt
//
LOCUS       DAOSXM010000048        15580 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2111
            NODE_2372_length_15580_cov_8.957166, whole genome shotgun sequence.
ACCESSION   DAOSXM010000048 DAOSXM010000000
VERSION     DAOSXM010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864178
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 15580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:24:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,261
            CDSs (total)                      :: 2,229
            Genes (coding)                    :: 2,218
            CDSs (with protein)               :: 2,218
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 3 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15580
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2372_length_15580_cov_8.957166"
                     /isolate="AMR_MDS_2111"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1304)
                     /locus_tag="PLQ68_07080"
     CDS             complement(<1..1304)
                     /locus_tag="PLQ68_07080"
                     /inference="COORDINATES: protein motif:HMM:NF012700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HXK72453.1"
                     /translation="MNEKYLNKIEYDKIINLLTKHVDSEPGKKTANEIHPMTDLDTIR
                     SSLEEISSSIYFVKAKGKPNFEGLREIDSIIKRLNVKGTLNNKELLIIKDNSYLARKA
                     YEASESISIYCKNEVDDIKTKMSSLVPLYDLESEIRRCIISEDDIADDASSELMRIRR
                     AIFKNQDAIKEKLISFTKSERYKKYLQESIITIRQDRYVLPVKNEYKNEVKGVVHDTS
                     QTGSTLFIEPIEIVNLNNKIRELLTEEKKEIEKILKLLSEQCAIYSEQISENFNTCCY
                     LDVAFGKASFALTYKGTVPEINNNRIIRLFNARHPLISPETVVPIDFKIGEDYTILVI
                     TGPNTGGKTVSLKTVGLLTAMTQSGLAIPVNEGSSVAVFSDIFVDIGDEQSIEQNLST
                     FSSHMTNIINILKQANSDSLVLLDELGAGTDPVEGSALALAI"
     gene            complement(1297..2061)
                     /locus_tag="PLQ68_07085"
     CDS             complement(1297..2061)
                     /locus_tag="PLQ68_07085"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HXK72454.1"
                     /translation="MIRAGLTSVTFRNLKPDEIIELAKATSVTDIEWGGDLHVKDESD
                     AVYVYGLCKKAGINCCSYGSYYKVGTYNDSYMIQFEKTLKTAIKLNCETIRVWCYNKP
                     SDDVERYSDEYNEIISEIRNIAKYAAKYKISISFEHHQKTLTDNHKRAIELISDVDYS
                     NVFMYWQAQVPLTFEENKQALTMFKPYLKNLHISNQIQSGYLLLSEMENQLLEYVNIV
                     KDKDTVALIEFVKGSDKTAYLKDIETLKKVLKKINE"
     gene            complement(2058..2573)
                     /locus_tag="PLQ68_07090"
     CDS             complement(2058..2573)
                     /locus_tag="PLQ68_07090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72455.1"
                     /translation="MRDKTKKLTKSAILLAVMMIFLYLRTIIPGYQLAFYFLASLVPG
                     IVVIEYGIKQGAIFVAASFLLSLIMPIDKLSFLLFYSFFGLYGIIKFLFEKISFRIVI
                     LASKIVYFTAVFFLNMYFGARIANIIPSVMLEYNIWIVLLVAIVVFVLYDIMYTSFSA
                     FYVNKIMRYIK"
     gene            complement(2593..3165)
                     /locus_tag="PLQ68_07095"
     CDS             complement(2593..3165)
                     /locus_tag="PLQ68_07095"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HXK72456.1"
                     /translation="MAISDPYKVLGVNRNDSDETIKKAYRELVKKYHPDKYMDNDLKD
                     LANEKLQQVNEAYDTIVKNRKSKDYGYSYDETSYKGTFAEVRSHIARGDFQTADNILR
                     NSTNRNAEWHYLRGVIFQKQGWYDQAYSYFEKAHVMEPSNAEYATAYNQTRSTANAYR
                     GNVYGRGYSSADDDFCRLCQCMLCASICCR"
     gene            complement(3167..4033)
                     /locus_tag="PLQ68_07100"
     CDS             complement(3167..4033)
                     /locus_tag="PLQ68_07100"
                     /inference="COORDINATES: protein motif:HMM:NF039826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5685 family protein"
                     /protein_id="HXK72457.1"
                     /translation="MIGYIKTYPPELKMKEYVKYNAYYCGICHSIKDRWGQIPRLTLN
                     YDCVFLAVFIDSLLKIPMEYEDFRCIVHPIVKKKKVIRSSTIDYISDMNLYLSYMKMV
                     DNVKDENNLFHSISEKTMRRYGKKLKEKYKEITDKIDNHLKDMQKLEDNNCDNIDEVA
                     ECYAEIIKDICLYKINEIDEETKCIVGKIAYNVGKWVYSADAFDDIEKDIKKNMFNPY
                     LARFQYEKGKDILKFKNDIKERSSFILCAPLKAATDLYEDLDDEYKDFIIRNILYEGL
                     YDRTEKILNGDK"
     gene            complement(4034..4576)
                     /locus_tag="PLQ68_07105"
     CDS             complement(4034..4576)
                     /locus_tag="PLQ68_07105"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HXK72458.1"
                     /translation="MKQIPNIITCIRLCLIPVFCYFLWNNNDLVAGIVFIIAALSDVV
                     DGYLARKLNAISNFGKLADPFADKIMQISAIILLFLLGRLKAWIMIVLFAKDFILMLG
                     GLLYKLIYNITVSSRWFGKISALTLNVIIAASILFSLGTGTTNILMVIAMCLQIASLV
                     AYTVLSLINLSKIKNNKKEA"
     gene            complement(4584..5546)
                     /gene="pfkA"
                     /locus_tag="PLQ68_07110"
     CDS             complement(4584..5546)
                     /gene="pfkA"
                     /locus_tag="PLQ68_07110"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011917349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HXK72459.1"
                     /translation="MKTIGVLTSGGDAPGMNAALRAVVRTGIAHNMTIYGINRGYEGL
                     INGEFEKLTARSVSGILQRGGTILRTARSERFREKTFRDRAMEMCNVFGIEALIVIGG
                     DGSYRGALDFLREGMKCVTIPATIDNDISSTEYCIGYDTALNTALSAIDKLRDTAYSH
                     ERVAVIEVMGRHAGYIAINDGIAGGADAIVVPELPYNFQKDIIKPIMEGRNRGKRDHC
                     IVIAEGVGDTAKICKDIEESTGIRTVCTILGYIQRGGSPTANDRLMASKMGYKAVEIL
                     NEDRYNKAICLKDNKIVDIDIEEALNMKKASFDYDMNLAKILAM"
     gene            complement(5586..9080)
                     /locus_tag="PLQ68_07115"
     CDS             complement(5586..9080)
                     /locus_tag="PLQ68_07115"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004617460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HXK72460.1"
                     /translation="MTDFVHLHVHTEFSLLDGSTKIQEMVSKAKEMGMKAVAITDHGV
                     MYGILQLYKSCISIGIKPIIGCEVYTARRKRTDKVYGLDSTQGHLVLLAKNNTGLHNL
                     MKIVSQSFEDGYYYKPRVDTELLRQYSEGIIALSACLSGEIPEAILEKDYEKAKNKAN
                     QYVDIFGKENFFLELQYNKIPEQSVVNQQLIKLSNEMDIPLVATNDVHYLDKEDAKAH
                     DLLLCIQTAKTVEDENRMKFSTDEFYFKSKDEMIKNFSNVPQAITNTQRVADMCNVNF
                     QLDEMHLPKFPLPDDVDHYDYMKNMVFEGLRRRYKEQLSEIHMKRAEYELEVINNMKY
                     IDYFLITADFINYAKNNGIPVGPGRGSAAGSIVAYAMNITNVDPIRYNLLFERFLNPA
                     RISLPDIDVDFCYERRAEVIEYVKRKYGSDKVCQIITFGTMAARGVIKDVGRALNLPY
                     ALCDKISKMIPNELKMKIDKALDVNPELAAEYENDPSVKELIDMAKRLEGISRHSGIH
                     AAGVVITDRPVKEYIPIQTSEEDSMVTQFPMENLEELGLLKVDFLGLRTLTVIKDCID
                     LIKKIHKVDINFDEMEYNDNNVFEYISQGNTSGIFQLESAGMTDFMKRFKPESIDDIT
                     LGISIYRPGPMQFIDMCLRNKNNPEGTEYEHDILKPILKDTYGCMIYQEQIMQICRDM
                     AGYTMGGSDLVRKMMAKKKMSALLKEKDNFVKGAEKNNVPTSVANKIFDEMIDFGNYA
                     FNKSHGVAYAYISYQTAYLKCYYPVEFMAATMNSFISAREKLAEYIEECKSINISVLP
                     PSINKSDIKFTVEGKDIRYPLAAINGVGMKACESIVNNRNEEGQFRSFTDFVTRINKS
                     DVNKKCFEGFIKAGAFDGLGNNRCELFSCYEDVVDTINNQQKKNLEGQLSLFEILDDT
                     DQFEYEIKRREEFDLSKKLIMEKEVLGLYMTGNPLDEYRGVLKDMNLFKCNCIKEDSE
                     TEEQQNLKLKDNERVKVAGIINSVQRKLTKNNDMMAFLQVEDLYGTIEVIVFPRTLDK
                     YASVLVPEKKVIINGRISLKQETVKLIAEDICELDIKKDECKNNDEINIDNMNGDNIG
                     LETNFIDPNKKLYINGNNIDLSSFEAFVKYFSGNSSLIVVKNIDGSKMIRTMYYNNEP
                     DIIMEMADMFGKENMAFK"
     gene            complement(9073..9705)
                     /gene="rdgB"
                     /locus_tag="PLQ68_07120"
     CDS             complement(9073..9705)
                     /gene="rdgB"
                     /locus_tag="PLQ68_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HXK72461.1"
                     /translation="MEDRTIIVASNNEGKINEIKAILPEYIVLSLKEAGIKSNPEETE
                     STFEGNALLKAKDAKRYTEHMVIADDSGLQVHSLNNIPGVFSKRFYFYTNSNSNKKST
                     INVKGKVLDKKNTKSLIDLMKNVKEKNRSASFVSVIALIEKDGTKKIFSGICEGKITM
                     ENKGNRGFGYDPVFIPNGYNITFAEMSDDEKNKISHRANALKQLKEYLND"
     gene            complement(9705..10415)
                     /gene="rph"
                     /locus_tag="PLQ68_07125"
     CDS             complement(9705..10415)
                     /gene="rph"
                     /locus_tag="PLQ68_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013007188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HXK72462.1"
                     /translation="MRLDKRKNNETRPITIKTGVMEYAEGSAYIECGKTKVLCTATVN
                     FESPPFRDSKGWITAEYNMLPRATIQRNKRDISSMKRNARGSEIERLIGRSLRACVDL
                     DAFNDATIVVDCDVIQADGGTRTASISAGYVALYKAFEWMIEKGYIQTNPIRTQIAAI
                     SAGIVNNEVMVDLCYDEDSNAAVDLNLVMDSNGQIAEIQATGEKSMFTKDQLDQIIEI
                     SSKSIDEIIKIQRKSLGM"
     gene            complement(10515..12059)
                     /locus_tag="PLQ68_07130"
     CDS             complement(10515..12059)
                     /locus_tag="PLQ68_07130"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HXK72463.1"
                     /translation="MDFNNDNSDKKKKIVTLVAVLLILCIVIAVIIFLLLNNDLWNNN
                     DTPSNTPSGTNSDNSLVPTDSSESFPTNTARIPKEINIRYENDVLIFDGLSKECEFTT
                     LFDSSKDYIFSEYRNPGDTKPTSWYEIDNKSVIYFTILLDQYNTYQFDENSIFPDNSL
                     VQKVEIEKNVENMSLDIKLITDRCIAVSDISTTQDSASYSLSEVKNMDFYNYSNVYSR
                     KFFFIPEYQLCKQSDSTVIYYEDNIDDGQNDVYSIRFKANAWSSLKGRNINYELDEEK
                     IYINDGYIEYIEVIKEEIGGVLYYRMSFLFQKELTLYPNANSLFSTLTLCDPNIENLI
                     FIDAGHGGHDPGTESRTDDKFNEADINLGICIKVEQLLLEKGYNVYMTRNEDIYLGFA
                     LERGDIANLHNAKLFLSVHVDSYTEESAKGILSIWRDDQDKPFAQVIQKNIVRTSFAS
                     DRGLMWQSLAVHGRTECPAVTIESGFITNPTESAKLKTDEYQQRIAQGIADGIIEYIN
                     IIDNNE"
     gene            complement(12075..14969)
                     /locus_tag="PLQ68_07135"
     CDS             complement(12075..14969)
                     /locus_tag="PLQ68_07135"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HXK72464.1"
                     /translation="MIKKLLRALLILILLLIPIKVIASQDIYSNCEYEIIDDVRYIKL
                     SSVAIVHKLTLQWSDSDKSVRTTYNDKNLIFSTNNNIVYINDDIYYFGEIKLIKGYTY
                     VPQDATVIFNQGYTAYLLDEPVEYKYINGRVTKIEVEKNNITTSVIKKYLINQMEYFC
                     IDELAQFFNIEPVKAHNNDYIMLMNNEHEVYFYVQSDNINLNGTNITTDNIIYMDNRY
                     YAPNNLLDFLIETFVTDYNNETDNPTSSSEDESLYTTYIFDDTSVKYVSMKQVSDIAG
                     YNYQWYYMNRYAIIERDNDKFFILVPSSIIMKDDLLYKQYSIKEDNDIVYIEYRALEV
                     FGLIHKDDIEDPANEVLPDEDDQNPSESDLETKLPSGYIPIRQFAEYINGTVMWDSEN
                     FSAILTHKDTEYTFDTRNLKIYVNEIEVSGYDFKFEDGITHVNTSLEELTSSTNILYK
                     EYEEYDLIYIKSPTATLKTHYPLSNPNRIVVDIADCDSVNNSESVKGQFFNSIRVLNE
                     PDNLKRIVFDLISKNNYEISKTKGVLEVKVFKTGTNNTQPSSSEQSGIVPGSTINDEN
                     ENTKPNNKADIKVETDKIIINTLDYENYEIKRISDPNRIVLFIPNSFSDIKEFPIKEN
                     SRYVKKVTSYYSDKGLYIEVELSNQCRYEIIEISETKLVMDIYSQKIINMSYYNYSDR
                     KYINITGLSLADQYGVVNSNVKVAKNEMITTIEFIDSNYRLTAGVLYVNDDFIEKIQV
                     KRINEKVIMTIFAKQKIDLYFNSARTSYTNINFIPENMTYSGSVVIDAGHGGYDPGAV
                     ASSVYESNINLKIALKVQTILENNNINVFMMRKTDEYVGLYERAHVANEIDASLFVSI
                     HSNAFSNSAYKGIMTLVYPSTVQSNKLSGKQFGTVLQKNLIKATSAIDRGVIDRPNLI
                     VLNSTKMPAALVECGFMTNAEELVALQSEEYQDILAQGIAAGIIEALSLK"
     gene            complement(15094..15396)
                     /locus_tag="PLQ68_07140"
     CDS             complement(15094..15396)
                     /locus_tag="PLQ68_07140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72465.1"
                     /translation="MTKKVYPDEYYKKKAIKLVVAHLRRKLEDMEHKEAGTFESWLTN
                     MDSLLEKNEFVMAEYVEQRKELNNLIDRLYDVDLRYKVRDSWNSFGKSLDKKAPTK"
BASE COUNT         4775 a         2837 c         1917 g         6051 t
ORIGIN      
        1 ataatagcta aagctaaagc agatccttct acagggtctg ttcctgctcc aagttcatct
       61 aaaagcacca gactgtcaga atttgcttgt ttcagaatat taatgatatt agtcatatgt
      121 gaggaaaatg tacttagatt ctgctctata ctctgttcat ctccaatatc aacgaaaata
      181 tcactaaata ctgcaactga actcccttca tttacaggaa tagctaatcc tgattgcgtc
      241 attgcagtta aaagtcctac agtcttcaac gatactgttt tacctcctgt attaggacct
      301 gttattacaa gtatagtata gtcttcacct attttaaaat ctataggtac tactgtttcc
      361 ggacttataa gaggatgtct tgcattaaaa agtctgatta tcctattatt atttatttct
      421 ggcacagtcc ccttgtatgt taatgcaaat gatgctttac caaacgctac atcaagataa
      481 cagcatgtgt tgaagttttc tgaaatttgt tcactataga tagcacactg ttcagacaat
      541 aattttagaa ttttttcaat ttccttcttt tcttctgtta atagctctct tattttatta
      601 ttcagattta caatttctat cggttctata aataaagttg aaccagtctg agatgtgtca
      661 tgaactacac ccttaacttc attcttgtac tcattcttaa caggaagcac atatctatcc
      721 tgtcgtattg ttattatgct ttcctgtaga tattttttat atctttcact tttagtaaaa
      781 gaaataagtt tttcttttat tgcatcctga ttcttgaata tagcccttct aatcctcatc
      841 agctctgatg atgcgtcatc tgctatatca tcttcagata tgatacatct tctaatttca
      901 gattcaaggt cataaagagg aactagtgag gacattttag ttttaatatc atccacttca
      961 ttcttgcaat agatacttat actttctgag gcttcatatg cttttctagc aaggtatgaa
     1021 ttatctttta ttattaaaag ttctttatta ttaagagtgc ccttaacatt taatcttttt
     1081 attattgaat cgatttcgcg caagccttca aaattaggtt ttccctttgc ttttacaaaa
     1141 tatattgaag aacttatttc ttcaagactt gatcgtattg tatccaaatc tgtcataggg
     1201 tgaatttcat tagctgtttt ttttcctggt tctgaatcaa catgctttgt tagaagattt
     1261 ataatcttat catattcgat tttatttaaa tatttctcat tcatttattt ttttaagtac
     1321 ttttttaaga gtttcaatat cttttaaata tgctgtttta tctgatccct taacaaactc
     1381 tattaaggca actgtatctt tatccttaac tatatttaca tactcaagta attgattttc
     1441 catttctgat agtaaaagat aacctgactg tatctggttt gatatatgaa gattttttaa
     1501 ataaggctta aacattgtca aagcttgttt attctcttca aaggtcaaag gcacctgagc
     1561 ttgccaatac ataaatacat tgctgtaatc aacatcacta attaattcaa ttgctctttt
     1621 atgattatca gtaagagtct tttgatgatg ttcaaaagaa atggagattt tatattttgc
     1681 tgcatatttt gcaatattcc ttatttcaga aataatttca ttgtattcat ctgaatatct
     1741 ttcaacatca tctgacggtt tattgtaaca ccataccctt atagtctcac aattaagctt
     1801 gatagctgtt tttagagtct tttcgaattg aatcatatat gaatcattat atgtgccaac
     1861 tttgtaatat gaaccgtatg agcagcaatt tatacctgct ttcttgcata aaccgtatac
     1921 ataaacagca tcagattcat ctttgacatg taaatctcca ccccattcaa tatcagtaac
     1981 tgaggttgct tttgctaatt caattatttc atctggtttc agatttctga atgtgactga
     2041 tgttaatcct gctcttatca ttttatatac ctcataatct tatttacata aaaagctgaa
     2101 aagctagtat acataatatc gtatagtaca aaaacaacaa ttgcaaccag taaaactatc
     2161 catatgttat attcaagcat aacagaagga atgatgtttg ctattcttgc tccgaaatac
     2221 atattaagaa aaaatacagc agtaaaatat actatttttg atgctagaat aactattcta
     2281 aaacttattt tttcaaatag aaactttata attccatata atccgaaaaa agaataaaat
     2341 agaagaaacg ataatttgtc aattggcata ataagagata gcaaaaaaga ggctgctaca
     2401 aagatggctc cttgctttat cccatattca attacaacta taccaggaac taaacttgca
     2461 agaaaataga aagccagctg gtaaccaggt attatcgtac gaaggtataa aaatatcatc
     2521 ataacagcaa gaagaattgc actttttgtt aatttttttg ttttatctct catttattat
     2581 taactctcat aattatctgc aacatatact ggcacatagc atacactggc ataatctgca
     2641 gaaatcatca tcagcagaac tatatcctcg tccataaaca tttcctctgt atgcattagc
     2701 agtacttctt gtctgattat atgcagtagc atattcagca tttgatggct ccataacatg
     2761 cgctttttca aaatatgaat atgcctgatc ataccatccc tgcttttgaa aaataacacc
     2821 tcttaagtaa tgccactcag catttctgtt tgttgaatta cgtaatatat tatctgctgt
     2881 ttgaaaatca cctctggcta tatgacttct tacttctgca aaagttcctt tatagcttgt
     2941 ttcatcatat gaatacccgt aatctttact ttttcggttt ttaactatcg tgtcatatgc
     3001 ttcattaacc tgttgaagtt tttcatttgc taaatccttc aaatcattat ccatgtattt
     3061 atcaggatgg tattttttta ccagctctct gtaagccttt tttatagttt cgtcactgtc
     3121 attgcggttt acgcctaaaa ccttataagg atctgatata gccaatttat ttatctccgt
     3181 tcaatatttt ttctgttctg tcatataatc cttcatataa aatgtttctt ataataaaat
     3241 ctttatattc atcatccaaa tcttcatata aatcagtagc tgcttttaat ggtgcacata
     3301 atatgaaaga ggaacgttcc ttaatgtcat tcttaaactt aagtatgtct tttccctttt
     3361 catattgaaa acgtgccaga taagggttaa acatattttt cttaatatct ttttctatgt
     3421 catcaaatgc atctgctgaa taaacccatt ttcccacatt ataagctatt tttccaacaa
     3481 tgcatttagt ttcttcatct atctcgttaa ttttatataa acagatgtct tttattattt
     3541 cagcataaca ctcagccacc tcatctatat tatcacagtt attatcttct agcttttgca
     3601 tatcttttaa atggttatca attttatcag taatttcttt atatttttct tttagcttct
     3661 ttccatatct tctcatagtt ttttcactta ttgaatggaa aagattattt tcatctttta
     3721 cgttatcaac catcttcata tatgatagat aaagattcat atctgaaata tagtctatgg
     3781 tactgcttct gatcaccttt ttctttttta caatcggatg aactatacat ctgaaatctt
     3841 catattccat tggaatcttc aacaatgaat ctataaatac tgctaaaaaa acacaatcat
     3901 aatttagtgt aagtcttgga atctgccccc atctatcttt aatagaatgg cagataccac
     3961 aataataggc attgtatttg acatattctt tcatcttcag ctctggtgga tatgtcttga
     4021 tgtaaccaat catctatgcc tccttcttat tattctttat cttactcaaa ttaatgagac
     4081 ttaatacggt atatgcaacc aaagaagcaa tctgtaagca catagcaatt accataagta
     4141 tatttgttgt tccagtacct aaactgaata aaatagatgc tgcaataatt acatttaggg
     4201 taagtgcaga tatcttccca aaccatcttg atgatactgt tatattatat attaatttat
     4261 aaagcaatcc tcctagcatc aatataaagt ctttagcaaa tagaacaatc ataatccatg
     4321 ctttcaatct tcctaataaa aacagaagta tgattgctga aatctgcatg attttatcag
     4381 caaaagggtc tgcaagtttt ccaaaattac taatagcatt tagttttctt gctaaataac
     4441 catcaacaac atcagatagt gcagcaataa taaacacgat gcccgctaca agatcattat
     4501 tattccataa gaaataacaa aatactggaa tcaggcaaag ccttatacaa gttattatat
     4561 ttggtatttg tttcatacta cctctacata gctaaaattt tagcaagatt catatcatag
     4621 tcaaaagatg cctttttcat atttagagct tcttcaatat caatatcaac aatcttgtta
     4681 tcctttagac atatagcttt attatatctg tcctcattca atatctcgac tgccttataa
     4741 cccattttac ttgccatcaa tctgtcattt gctgttgggc ttccacctct ttgaatgtat
     4801 cctagtattg tgcaaacagt tctaatacct gttgattctt caatatcttt gcatattttt
     4861 gctgtgtcac caactccttc tgcaataact atacagtggt ctcttttccc tctgtttcta
     4921 ccttccatta ttggttttat aatatccttt tgaaagttat atggaagttc aggtactact
     4981 atagcatctg caccgccggc aatgccatca tttattgcta tatatcctgc gtgccttccc
     5041 ataacttcga taactgctac tctttcatgc gaatatgcag tatctcttaa tttatcaata
     5101 gctgaaagag cagtatttaa tgcagtatca taaccgatac agtactctgt tgacgaaata
     5161 tcattatcta tagttgcagg gattgtaaca cacttcattc cttcacgaag aaagtctagt
     5221 gctcctctat atgaaccatc cccaccgata acaataagtg cttctattcc gaatacatta
     5281 cacatctcca tcgcacgatc acggaatgtt ttttccctaa atctttctga tcttgctgtt
     5341 ctcaaaatcg taccgcctct ttgtaaaatc ccagagacgc ttcttgctgt aagtttttca
     5401 aactctccat taataaggcc ttcgtatcct ctattaattc catatatcgt catattatga
     5461 gcaatacctg ttctaactac agctcttaat gcagcattca ttccgggtgc atccccgcca
     5521 cttgtcaata ctcctattgt ttttatactc attatcatat ctcctggtta aatattatca
     5581 caaaattatt taaatgccat attttcttta ccaaacatat cagccatctc cataattata
     5641 tctggttcat tattataata cattgtcctt atcattttac ttccatcaat attttttaca
     5701 acaattaatg atgaattgcc cgaaaaatat ttaacaaatg cttcgaaaga agacaaatca
     5761 atattattgc cattaatata tagtttttta tttggatcaa taaaatttgt ttctaaacca
     5821 atattatccc cattcatatt atctatattg atttcatcat tatttttgca ttcatctttt
     5881 ttaatatcta gctcacaaat atcttctgca attaacttca ctgtttcttg ctttaaacta
     5941 attcgaccat ttattattac ctttttttct ggcactaaaa cagatgcata tttgtccaat
     6001 gtacgaggaa atactataac ctcgattgtt ccatatagat cctcaacctg taaaaatgcc
     6061 atcatatcat tattttttgt aagtttacgt tgcacagagt taataatacc tgctactttt
     6121 accctttcat tatcttttaa ttttaaattt tgttgttctt ctgtttcaga atcttctttt
     6181 atacaattgc atttgaaaag attcatatct tttaaaacac ctctgtactc atccagtgga
     6241 ttaccagtca tatataatcc taatacttct ttttccatta ttaatttttt tgaaagatca
     6301 aattcttcac gtctttttat ctcatattca aactgatctg tgtcatctaa aatttcaaat
     6361 aaagacaact gaccttccaa atttttcttt tgttgattat taattgtgtc aacaacatct
     6421 tcataacatg aaaatagttc acatctgtta tttcctaaac catcaaaagc accagctttt
     6481 ataaaacctt caaagcattt tttgttcaca tcacttttat ttattcttgt tacaaaatca
     6541 gtaaagcttc taaattgtcc ttcctcgttt ctattattta ctattgattc acaggctttc
     6601 attcctacac cattgattgc agctaatgga tatcttatat ctttcccttc aacagtaaat
     6661 ttaatatcac ttttgttaat gcttggtggt agtactgaaa tgttaattga tttacattct
     6721 tcgatatact ctgctagttt ttctcttgca gatataaaac tgttcatagt cgcagccatg
     6781 aattctacag gataataaca ttttaagtaa gccgtctgat atgaaatata tgcatatgct
     6841 acaccatgtg atttattaaa agcataatta ccgaaatcta tcatttcgtc aaatatctta
     6901 tttgctacac ttgtaggaac attattcttt tctgcacctt ttacgaagtt gtctttttcc
     6961 ttaagaagcg ctgacatctt cttttttgcc atcatttttc ttacaagatc agatcctccc
     7021 atagtatatc cggccatatc acggcatatc tgcattatct gttcctgata aatcatacat
     7081 ccgtaggtat ctttcaaaat tggttttaga atatcgtgtt catattcagt accttctgga
     7141 ttattcttgt ttcttaaaca catatctata aactgcatag gacctggccg gtatattgaa
     7201 attccaagag ttatatcatc tatagattct ggtttgaaac gcttcatgaa atctgtcatt
     7261 cctgcgcttt ctagttggaa tattcccgat gtgttacctt gtgagatgta ttcaaataca
     7321 ttattgtcat tgtattccat ttcatcaaag ttaatatcaa ctttatgaat tttcttaatc
     7381 aaatcaatac agtcttttat aactgtaaga gtacgcaaac ctaagaaatc aactttaagt
     7441 aatcccagct cttctaaatt ctccatagga aattgtgtta ccatagaatc ttcttctgat
     7501 gtctggatag gtatgtattc ttttacaggc ctgtctgtta ttactacgcc tgcagcatgt
     7561 attccactgt gccttgatat tccttcaagt cttttcgcca tgtcaattag ttcttttacc
     7621 gatggatcat tctcatattc cgctgctaat tcgggattta catcaagagc tttgtctatc
     7681 ttcattttta attcattcgg tatcatttta cttattttgt cgcataaagc ataaggcaga
     7741 tttaaggctc ttccaacatc ctttattact cctctagcag ccatagtacc aaaagtgata
     7801 atctggcata ccttatctga accatacttt ctttttacat attcaattac ttctgctcgt
     7861 ctttcgtaac agaaatccac atcaatgtca ggtaaagata ttctagccgg attcaaaaat
     7921 ctttcaaaaa gaagattata tcttatcgga tctacattag ttatattcat cgcgtatgct
     7981 actatactgc ctgctgcact tcccctacca ggtcctacag gtataccatt atttttagca
     8041 tagtttatga aatcagcggt tataaggaag taatcaatat atttcatatt atttattact
     8101 tccaattcat attctgctct tttcatatgt atttctgata attgctcttt atatcttctt
     8161 cttaaacctt caaataccat atttttcatg taatcataat gatctacatc atcaggaagt
     8221 ggaaactttg gaagatgcat ctcatcaagt tgaaaattta cattgcacat atctgcaaca
     8281 cgctgagtat ttgtaatcgc ttgaggtaca tttgaaaagt tttttatcat ttcatctttt
     8341 gatttgaaat aaaactcatc tgtagaaaac ttcattctgt tctcatcttc tacagtcttc
     8401 gctgtttgta tacataaaag aagatcatgt gcttttgcat cttctttgtc tagataatga
     8461 acatcatttg tagctacaag cggtatatcc atttcattgg ataattttat aagctgttga
     8521 ttaacaactg actgttcagg aattttgtta tattgaagtt ctaaaaaaaa gttttcttta
     8581 ccaaatatat ctacatattg attcgcttta tttttagcct tttcataatc tttttctaga
     8641 atggcttctg gtatttcacc agatagacac gcactcaaag caataatacc ttcactatat
     8701 tgtcttagaa gctcagtgtc tacccttggt ttatagtaat atccatcttc aaatgattgg
     8761 gatactatct tcattaaatt atgtaatcct gtattatttt ttgctaatag tactaggtgt
     8821 ccctgagttg aatccaaacc ataaaccttg tctgttcttt ttcttctagc tgtataaact
     8881 tcgcagccta taatgggttt tatgcctatg cttatacaac ttttgtacaa ttgaagaatt
     8941 ccgtacataa ctccatgatc agttatggcc acagctttca tacccatttc ttttgcttta
     9001 ctgaccattt cttgtatttt tgttgatccg tccaaaaggc tgaactcagt atgaacatga
     9061 agatgtacaa aatcagtcat tcaaatactc cttaagttgt tttaatgcat ttgctctatg
     9121 acttatttta ttcttttcat catcactcat ttcagcaaat gttatattat atccatttgg
     9181 aataaacact ggatcatatc caaatcctct attgccttta ttttccatag taattttacc
     9241 ttcacagatt ccagaaaata ttttttttgt cccatctttt tcaattaatg caattacact
     9301 tacaaaactt gcacttctat tcttttcttt cacatttttc ataagatcta ttaaggattt
     9361 tgtattcttt ttatctaata ctttaccttt cacatttatc gtgctttttt tatttgaatt
     9421 actatttgta taaaaataga atcttttgga aaaaacacct ggtatgttat ttaatgaatg
     9481 tacctgtaac cctgaatcat cagcaataac catatgttcg gtatatcttt tagcatcttt
     9541 agcttttaac aaagcatttc cttcaaaagt gctttctgtt tcttctggat ttgattttat
     9601 tcctgcttct tttaatgata atactatata ttcaggcaga attgccttaa tttcatttat
     9661 ttttccttca ttattacttg caacaattat tgtcctatct tccattacat acctaaactc
     9721 tttctttgta ttttaattat ttcatctatt gatttacttg atatttcaat tatttgatca
     9781 agctgatctt ttgtaaacat acttttttcg ccagttgcct gtatctctgc aatttgtccg
     9841 tttgaatcca tgacaagatt tagatctacc gcagcattac tgtcttcgtc ataacaaaga
     9901 tcaaccatta cttcgttgtt aacaatacca gcacttattg cagctatctg agttcttatt
     9961 gggtttgttt gaatatatcc tttttctatc atccactcaa atgctttata taaagctaca
    10021 tatccagctg atatactggc tgttcttgtt ccaccatctg cctgaattac atcacaatct
    10081 acaacaatag ttgcatcatt aaaagcatca agatccacac atgctcttaa tgaacgtcct
    10141 attaatcgct caatttcaga acctcttgca tttctcttca tactggaaat atccctttta
    10201 tttctttgaa tcgtggctct tggaagcata ttgtattcag cggttatcca tcctttagaa
    10261 tctctaaacg gaggtgattc aaaattcact gtagcagtac atagtacttt tgttttacca
    10321 cattcaatat atgccgaacc ttcagcatat tccataactc cagttttaat tgttataggt
    10381 cttgtctcgt tgttttttct tttgtctaaa cgcataaatc accttttcta aattatattg
    10441 aaataacatt aaattaatgt taactaaata caattttaac atttttatgc tactcataca
    10501 aacagaaaag tatattattc gttattatca ataatgttta tatattcgat tataccatca
    10561 gcaataccct gagcgattct ttgctggtat tcatctgttt taagttttgc gctttctgta
    10621 ggatttgtaa taaatcctga ctcaatagta actgcaggac attcggtacg tccatgtaca
    10681 gcaaggctct gccacataag tcctctgtct gatgcaaatg atgtacgtac aatatttttt
    10741 tgaataactt gagcaaatgg cttatcctgg tcatctctcc atatagaaag tataccttta
    10801 gctgattctt cagtatagga atcaacatga acagataaga aaagtttagc attatgaaga
    10861 tttgcaatat cccctctttc aagagcaaaa ccaagatata tgtcttcatt tcttgtcatg
    10921 tatacattgt atcctttttc aagcagtaac tgctctactt ttatacaaat acccaaattt
    10981 atatctgctt cattaaattt atcatctgtt ctggattctg ttccaggatc atgtccgcca
    11041 tgacctgcat caataaatat tagattttca atatttggat cgcataatgt caaagttgaa
    11101 aacagtgaat tcgcatttgg atataaagtt agttcttttt gaaaaagaaa actcatacga
    11161 taataaagta ctccgcctat ttcctctttt ataacttcta tatactctat atagccatca
    11221 ttaatatata ttttttcttc atcaagttca taatttatat ttcttccctt aagtgaagac
    11281 caagcattag ccttaaatct tatagaatat acatcattct gaccatcatc tatattatct
    11341 tcataataaa ttacagtgct atctgattgt ttgcataact ggtattcagg aataaaaaag
    11401 aattttcttg aatacacatt cgagtagtta taaaaatcca tattttttac ttcagatagt
    11461 gaatatgacg cactatcctg cgttgtagaa atatcagaaa cagcaatgca tctatcagtt
    11521 attaatttaa tatcaagtga catgttttca acatttttct caatttccac tttctgaact
    11581 aaactgttat ccggaaatat agagttttca tcaaactggt atgtattata ttgatctaat
    11641 aaaattgtaa aatatattac tgatttatta tctatctcat accaagatgt aggcttggta
    11701 tcacccggat ttctgtattc actaaaaata taatctttac tactatcaaa gagtgttgta
    11761 aattcacatt cttttgataa accatcaaag attaaaacat cattttcata gcggatattt
    11821 atctctttag gtattcttgc tgtattagtt gggaatgatt ctgacgaatc tgtaggtacc
    11881 aatgaattat cagagtttgt acccgatggt gtatttgaag gtgtgtcgtt gttattccac
    11941 aaatcattat ttaaaagcaa aaatattata acagcaataa caatacataa aattaataat
    12001 actgcaacta atgttactat tttctttttt ttatctgaat tatcgttatt aaaatccata
    12061 aagcctctct gttttcattt taagcttaat gcttctatta ttcctgctgc aattccctga
    12121 gcaagaatat cctggtattc ttctgactgt aatgctacta attcttcagc gtttgtcata
    12181 aatccgcatt caactaaagc tgctggcatc tttgtagagt ttagcactat taaattaggt
    12241 ctgtcaataa ctcccctatc aattgcagac gttgctttta taagattctt ctgtaaaaca
    12301 gtaccgaact gttttccgct taatttattt gattggacag tagaaggata cactaaagtc
    12361 attattccct tataggctga attactaaaa gcatttgaat gaatacttac aaataaagaa
    12421 gcatcaattt catttgctac atgagctctt tcgtataatc caacatattc gtctgtcttt
    12481 ctcatcatga aaacattgat attattattc tcaagtatgg tttgcacctt tagagcaatc
    12541 tttaaattta tattagattc atataccgaa gaagcaactg ccccggggtc ataccctcca
    12601 tgccctgcat ctatcactac cgaacctgaa tatgtcatat tttcaggtat aaagttaata
    12661 ttcgtatatg acgttcttgc tgaattaaag tacaaatcaa tcttttgttt tgcaaatata
    12721 gtcataatta ctttttcatt aattctttta acctgtattt tttcaataaa atcatcatta
    12781 acatatagta cacctgcagt cagtcgataa tttgaatcta taaattcaat ggttgtaatc
    12841 atctcatttt ttgctacttt aacatttgag tttacaacac catattggtc tgccaatgat
    12901 aatccggtaa tatttatata ttttctatca gaataattgt agtatgacat attaattatt
    12961 ttttgagagt atatgtccat tactaacttt gtctcactta tttctattat ttcatatctg
    13021 cactgattgc taagttcaac ttcaatatat aaacctttat ctgagtaata tgaagttact
    13081 tttttaacat accttgaatt ttctttaatc ggaaattcct ttatatcact aaaggaatta
    13141 ggtatgaaaa gtactattct atttggatca gatattcttt ttatttcata attttcataa
    13201 tccagcgtgt ttattataat tttatctgtt tctactttaa tatccgcttt attatttggt
    13261 tttgtatttt cattttcatc attgattgta gatcctggaa caatacctga ttgttctgaa
    13321 gatgaaggct gtgtattatt agtaccagtt ttaaaaactt taacctctaa aacaccttta
    13381 gttttactaa tttcataatt gtttttggat attaggtcga aaacaattct ttttagatta
    13441 tcaggttcat tcaacactct tattgagttg aaaaactggc cttttacaga ctcagagtta
    13501 tttactgaat cacaatcagc tatatctaca acaattctgt ttggatttga taaaggataa
    13561 tgagttttta aggttgctgt cggtgatttt atatatatca aatcatactc ttcatattct
    13621 ttataaagta tatttgttga acttgtcaat tcctctaagc ttgtatttac atgtgtaatg
    13681 ccatcttcaa atttgaaatc ataacctgaa acttctattt catttacata aatttttaaa
    13741 tttcttgtat caaaggtata ttctgtatct ttatgtgtaa gtattgcact aaaattttca
    13801 ctatcccaca ttacagtgcc gttaatatat tcagcaaatt gtcttattgg tatatatcct
    13861 gaaggtaatt ttgtttctaa atcagattct gatggattct gatcatcttc atctggtaaa
    13921 acttcatttg caggatcttc aatatcatct ttgtgtataa gtccaaatac ttcaagtgcc
    13981 ctatattcaa tatatacaat atcattatct tctttaatgc tatattgttt ataaagcaga
    14041 tcatccttca taattatact ggaaggtact aatatgaaaa acttatcatt atctctttca
    14101 ataattgcat atctgttcat atagtaccac tgataattat aaccagcaat gtctgaaacc
    14161 tgcttcatgc ttacatattt tacagatgta tcatcaaata tatatgttgt gtatagtgat
    14221 tcatcttctg aactactagt aggattatcg gtttcattat tataatctgt tacaaaagtt
    14281 tcaattaaaa aatcgagaag attattcggg gcataatatc tgttatccat ataaattatg
    14341 ttatcagttg taatatttgt tccatttaaa ttgatattgt ctgattgaac atagaaatat
    14401 acttcatgtt cattgttcat taacattatg taatcattgt tatgtgcttt aactggttcg
    14461 atattaaaaa actgagctaa ttcatcaata cagaaatatt ccatttgatt tattaaatat
    14521 tttttaataa ctgaggttgt tatattattt ttctctactt ctatctttgt tactctgcca
    14581 ttaatatatt tgtactcaac aggttcgtct agaagatatg cagtataacc ttgattaaaa
    14641 ataacagtag catcttgagg tacatatgta taccccttaa taagctttat ttcaccaaaa
    14701 tagtatatat catcatttat atatactatg ttattattgg tactaaagat taaattttta
    14761 tcattatatg ttgttctgac gcttttatct gaatcactcc attgaagagt tagcttatga
    14821 acaattgcga cagaagataa tttgatatat ctgacatcat caataatctc atattcacaa
    14881 tttgaatata tatcctggct tgctattacc ttaattggaa taagcagtaa gattaaaatt
    14941 agaagtgccc ttagtaattt ttttatcatt ttatcttttg ttcctcatac tatttattta
    15001 attttacagc aaaaagtatt tataatcaac aaaaatatgc acaaaacata gttttctgca
    15061 gaattcatcg taactttaaa agatattcac aaatcatttt gtaggtgctt ttttgtcaag
    15121 gctcttgcca aaactattcc atgaatccct aaccttataa cgtaggtcga catcataaag
    15181 acgatctata agattattta gctctttcct ctgctctaca tactccgcca ttacaaattc
    15241 attcttttct aaaagtgaat ccatattagt cagccatgat tcaaatgttc cggcctcttt
    15301 gtgttccata tcttcaagct ttcttcgaag atgtgcgact actaatttaa tggccttttt
    15361 tttataatac tcatcagggt atactttttt agtcatattc aattaccagc cttctttatt
    15421 aatattatta aatttaagca ttgtacaatg tacaatcctg ttgcgtgtag cttgtctgtt
    15481 tacataaata tgatatcata tggtactata ataagcaata tagtaaattt atgctataat
    15541 tacataatat gaaaaagaat atctcagaaa aattaatata
//
LOCUS       DAOSXM010000049        15428 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2111
            NODE_2398_length_15428_cov_8.499772, whole genome shotgun sequence.
ACCESSION   DAOSXM010000049 DAOSXM010000000
VERSION     DAOSXM010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864178
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 15428)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15428)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:24:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,261
            CDSs (total)                      :: 2,229
            Genes (coding)                    :: 2,218
            CDSs (with protein)               :: 2,218
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 3 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15428
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2398_length_15428_cov_8.499772"
                     /isolate="AMR_MDS_2111"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..714
                     /locus_tag="PLQ68_07145"
     CDS             <1..714
                     /locus_tag="PLQ68_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005487584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HXK72466.1"
                     /translation="YQLATEGYDIAILDVFAQEDVKDNVDRIIKTGADVLYFQGDLSK
                     DESRKQFVESVVNHFGRIDVLVNNAGVGPKVRLDILETTEESFDFVLGINLKGTFFLT
                     QTVANEMIKLKDKFEDCNPKIVNISSMSAYTSSTSRGEYCISKAGVSMVTKLFADRLA
                     EHEINVYEIRPGIIYTPMTEVVRAKYDKLIAEGICPIKRWGYPEDIANVVSAFVSGKF
                     AYSTGEVINVDGGFHIQRL"
     gene            727..2697
                     /locus_tag="PLQ68_07150"
     CDS             727..2697
                     /locus_tag="PLQ68_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014553052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HXK72467.1"
                     /translation="MNRSTIKINNKEYDFITVNTVIVGTGAAGFNAADSLYDLGQEDI
                     CIVTEGRNMGTSRNTGSDKQTYYKLTLAGDGDDSVHEMAKTLFAGGSMHGDIALTEAA
                     LSAKGFYKLASIGVPFPHNKYGEYVGYKTDHDPRQRATSAGPLTSKFMTEKLEKQIES
                     KGIMIFDGYYVIKILQKDGSACGLITINNSKTDQDNFGITIFNCTNIVYATGGPAGIY
                     DTSVYPISHTGSTGIAFEAGAKGINLTESQYGIASIKFRWNLSGTYQQVIPRYISTDQ
                     EGNDEKEFLDEYFIDKGKLFDCIFLKGYQWPFDPRKIENIGSSIIDILVFNETAIKGR
                     RVFMDFRRNPKHFSFDLLNEESYTYLKNSNAFQSTPIERLEHMNKPAIELYQNNGIDL
                     YTENLEIAVCAQHNNGGLMGNIWYESNIKHLFPVGEVNGTFGVYRPGGSALNSTQVSS
                     FRAAQYISQNYTDNPSSSEEFIINWKDEINGCYELTEKLIDKKSNSNILDLRKRYQKL
                     MTKSGAHIRSLKEVSEAVNTSLQELKSFESVTKINNTSQISDAFRNRDILITQYVYLC
                     AIKEFIEKGGRSRGSYLVRDDKGSLPMEDLPEIFRFTLDDQQLMKKACSVSFNKEDLS
                     VKYEWSEVRPIPTEDNWFENVWSEYRKKNIIK"
     gene            complement(2700..3653)
                     /locus_tag="PLQ68_07155"
     CDS             complement(2700..3653)
                     /locus_tag="PLQ68_07155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72468.1"
                     /translation="MRTIFAIYITIALLLSSLHAVKPQQFFYDISIFSWSSSQLDPSE
                     FESVAELLKMNRINQIYQSFSENSIVNASASDFIKNMKINNIDTFYLSGDPSWATERR
                     NRSMRAKIDLIYEYNKKVDPNSQFSGIVFDVEPYLLSEWKEDSDRVMEKFLSNMVDTY
                     EYAKQFDISVIICIPYWFDNNYYDILERLISEACDKVAVMNYARNKEIKNIANEVNIA
                     KEYKKQIVCITEIQPPSGTSIPDSITYYNYGLNVIWRMWNQVYSYFDYDDLSFSYHQY
                     KQLKEFINDLENSYKPAQIVKRPHITYDLLHDGKTYISLIK"
     gene            complement(3741..5096)
                     /locus_tag="PLQ68_07160"
     CDS             complement(3741..5096)
                     /locus_tag="PLQ68_07160"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010248161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HXK72469.1"
                     /translation="MLKVVKFGGSSLADPSQVKKVFDIVTSDPSRKFVVVSAPGKRYP
                     QDTKVTDMLIKLAEKSLAHDDINNQLNLIVQRFKDISEGLGMPDQIIEIIRADLIDRV
                     NYDKSVKERYLDNLKAAGEDNCAKLVAEYVNFKGHKANYVSPKDAGLLLTSEFGNAQI
                     LPVSYENLKILNDYEGIVIFPGFFGYSVNQEVVTLPRGGSDVTGSILAAALDCDLYEN
                     FTDVDYIYTVNPNIVKNPLPIDHLTFKEMRELSYAGFSVFHEEALTPVYYKFTHVNIK
                     NTNNPSAPGTMIHREVNENRKHRITGISGDKGFSTISVRKYLMNREIGFGRKILEMLE
                     DEMIPYEHSPSGIDDISIIVRSKYLNEQIEDRLICRIRNELKAEEVSIIHHQALVMIV
                     GEKCVDDSHTLHLCSEALSEENIVIKMLNQGSSKLSIMVGIDEIDFEKAIQCLYKKFF
                     T"
     gene            complement(5204..6637)
                     /locus_tag="PLQ68_07165"
     CDS             complement(5204..6637)
                     /locus_tag="PLQ68_07165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72470.1"
                     /translation="MKKQIARKFLFFDYWTYETLSGFTRKTHKPVKFKNNPVFVANNN
                     QYEYNSCSYSTVSYDYDEKIFKMWYGTSNLYKTDEEAIKYLCYATSSDGIKWDRPILD
                     VIDGTNVVMDKDMYPMGASVIIDKEDKNMPYKLIMRPKNIPAIATCFSKDGIHWDKKN
                     INIVINADSDCKIGLYKNPVERTYYALFRNIKGQRKNYISSSKDFINWDFPKLILEPD
                     ISEGCQTQIYGTQASSYGNYVIGLSPLYNTVETDMNWAKMEGTMDISAAFSKGSYNWR
                     WLNPKNRFISLKDEKTSECGMIHPLTSLIYLPEKIRMYYAASDFSHGNTHLITQKPQY
                     INFAELRPDGFTSIACDEAGYLLTRPFSVSSSQLYINANCNKGTLRASIRDAYTNQPI
                     EGFNFKDCIAINTDEIYHKIEWKNNDCVNRFENRPIRLAIECKNVEIFSITFPNNNDV
                     KKYWEFDEITCVNPKKDISEDDYFMKI"
     gene            6791..7693
                     /locus_tag="PLQ68_07170"
     CDS             6791..7693
                     /locus_tag="PLQ68_07170"
                     /inference="COORDINATES: protein motif:HMM:NF012424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HXK72471.1"
                     /translation="MKRRIGDRYDGYKLRNVDTFFLLIPYIMKKRDDSQIFFEDVADV
                     DLLDAFIRKHQEDIPGLKLYHIFIATLVRLFALRPRINRFVCGSKIYARKEIAISMSI
                     KRSMTDDGEETNIKPIFAPESTLKDIVDIVNEQVNMNKETNSENDTDKFAKVVGHMPT
                     WLIKAVVNTLMWMDRKGLMSKKIIDFSPFHCSGYLTNMGSLGIKAVYHHLYNFGTTSV
                     FLAMGKKEYQYQGSDEGELIKKRIISLKMVVDERICDGYYYASTMRLFSKILQNPEVL
                     LMPPEEIPVDDGIVREKKKKVKKA"
     gene            7698..8453
                     /locus_tag="PLQ68_07175"
     CDS             7698..8453
                     /locus_tag="PLQ68_07175"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXK72472.1"
                     /translation="MKSKLLKIAAVMLWLIVWQILSMVINKELLMPSPYVTISTLVRL
                     MSDSSFYYAMLYSLLRIISGFLVAAALAFILGIIAGLNRTVEILISPFITIVRSTPVM
                     SFIILAIIWFSYNLAPLVVNLIMCFPPIYDNVVKGIKNTDRKLLDMAKVYKVKPFNVF
                     KGIYLGSLMPFLSAGMNTALGLAFRVAVAAEVLANPRYGIGTRLLQAKYNLETAEVFA
                     WTFMILLLSYIFEYIFKLIIEGGRHYAQNKKSD"
     gene            8428..9129
                     /locus_tag="PLQ68_07180"
     CDS             8428..9129
                     /locus_tag="PLQ68_07180"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXK72473.1"
                     /translation="MLKIRNLTKSYNGNQIFRNFDLDIEKNRITCLLGKSGSGKTTLL
                     NVLSSITDEYTVDYNDFKNLTYSYIFQEPRLLNWHTVFKNMLFVLDGKEAKSKEAHNK
                     IMNYLKMVEMDKYKDYYPDQLSGGMAQRVSIARAFIKDFDVLLMDEPFKGLDPTQKED
                     IMDEFLNMWKDNRKTVLYVTHELDEALYLAYDIYVFGGKPAKIVYTCKNAYKMTDKER
                     EIEREKIWQILKLQI"
     gene            9102..10058
                     /locus_tag="PLQ68_07185"
     CDS             9102..10058
                     /locus_tag="PLQ68_07185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HXK72474.1"
                     /translation="MADIKITDLTVGMEIKNELYILWYNELKKASNNKFYMDFQFRDK
                     TGEINGKKWDATPNELNDYNNAELYFVTGRVTEFQGSLQLNVSSLRKAPQQIQKNIAD
                     FVQSAPVSSDDMVNKVYEFINLIKDEELKKLCTQVYQENIEQIRFYPAAKALHHSVRG
                     GYLYHITTMLDLAKAISNVYTTVNTDFLYSGILLHDIMKLKEMNANECGIADYSTEGQ
                     LMGHIEMGICYIGEMAKKVGLSQNKELLIKHMILSHHYIPEHGSTKMPMFLEAELLHH
                     IDLIDARTYDFNKAYETLQKGEITERTVFSLDRKVMNPDFEK"
     gene            complement(10089..10164)
                     /locus_tag="PLQ68_07190"
     tRNA            complement(10089..10164)
                     /locus_tag="PLQ68_07190"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10129..10131),aa:Lys,seq:ctt)
     gene            10279..11430
                     /gene="dinB"
                     /locus_tag="PLQ68_07195"
     CDS             10279..11430
                     /gene="dinB"
                     /locus_tag="PLQ68_07195"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012103770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HXK72475.1"
                     /translation="MKDILLVDMNAFFIMCESRRDNSLLNTPAAVAGDPKYRTGIILA
                     ANYEARKFNVKVTMTVNEALKICPNLKLVKPDHEYYEKCSKQVFSLLSEYSPQLEINS
                     IDEAWIDVTSSKKLFGDPYEIACDIQKRIKEELGIWCSIGISENKFLAKMASEMKKPL
                     GITKLYKSSVREMLWPLSINKLYGIGNATAKKLNKDGITTIGDLAKADLSYLVSRFGK
                     YGVYLYERSKGIDDDLVCPNDSKSLSCGRMETLTHDITDLNEAKRVIAVLSESIARSV
                     RQKNLVGSVVQIQIKYNTFKMISRQITIEETSLTKDIYNAGCSILENNWDQDKPVRLL
                     GISLCSLKQKDMQISFDVNGKQKEEHLEKTIDKLNSKFGQDIIKRGNTI"
     gene            11427..>11687
                     /locus_tag="PLQ68_07200"
     CDS             11427..>11687
                     /locus_tag="PLQ68_07200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HXK72476.1"
                     /translation="MKIVLFALNSSFVHTNLAIRCLKDYIDKNSLKKHDVLLVEKNHK
                     DKFDEILHSLHECNADLYGFSCYIFNIDEIIQIAKSLKKLLPN"
     assembly_gap    11688..11787
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <11788..12715
                     /locus_tag="PLQ68_07205"
     CDS             <11788..12715
                     /locus_tag="PLQ68_07205"
                     /inference="COORDINATES: protein motif:HMM:NF024707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4080 domain-containing protein"
                     /protein_id="HXK72477.1"
                     /translation="NCSYCLSSTTKGIRSKTSQKTLDDLYEFEKFGSKIKIIKFVDRT
                     FNFDIERAKIILNGLTDIKYSKIYHMEMRPELFDDEMFEILARFPKDKLQLEIGIQST
                     NKKTLEAIDRPSDINKAISNMKRIRISNNIKIHCDLIAGLPFDNINTIRQSYNDVFFA
                     CDELQLGFLKMLKGSKIRKQADKFNYIYNDKAPYEVLSNDCISYEMMFKLKRIAALTQ
                     RFKNSMHFKKSCEYIVSFNISPFDYFYGFNEYIGEHISHLSQHEAFKKFFEYSSFNIK
                     NSISVDEWDKFIKILCEEYKQCLNKNAPEYFC"
     gene            complement(12712..14019)
                     /locus_tag="PLQ68_07210"
     CDS             complement(12712..14019)
                     /locus_tag="PLQ68_07210"
                     /inference="COORDINATES: protein motif:HMM:NF018004.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HXK72478.1"
                     /translation="MDFIEKRLLDCAVHAPILYIPDNPSDHILWPSFPFTYNNLNEKL
                     IDPLDIGFLMNSKDYTPQYNSDNVKLTKNHFNKINKNVKRSLRSGFLSDYEGFVLTRH
                     IYPSGEERYGLLVLLDLEQYSNQPLSIISYAKDFDEDKAKRHSEFLNDSVIDFPFSQA
                     FIEDKSFSLIEPLISCTFNFKLIFSFDHPIYGVYEGYQISTPQHYTLISNALMKIKEK
                     VIIENKPMFIISDGIESFESAKIHWEWLKNKYGNDLYSLHPSRFQMVELLNISNNSTI
                     MNPFYIILKNTQIDDLVKQIRNNTKIKVESQISLKELNNAINKRFDKYKSVCYGMTDI
                     ESFYLITFRNPDSDIGIINILDVVNEYLVKRNNDICFYSNANEFYDNISQSQDCGIIL
                     PPINKDTFFKNIANLGKLSAYAYVSDIESSKNFIFEARKIIDD"
     gene            14238..15341
                     /locus_tag="PLQ68_07215"
     CDS             14238..15341
                     /locus_tag="PLQ68_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008726007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HXK72479.1"
                     /translation="MASKVYFTNMKTDHGQSLLQKFEKLIKKAGLSEIPFENKFTAVK
                     VHFGEPGNLAYIRPNYAKVLCDYIKHLKGRPFLTDCNTLYPGRRKNALEHLDTAYQNG
                     FNPFVTGCNVIIGDGLKGTDETLIDINMKHVKQAKIGHAVADSDVIISLNHFKGHVAT
                     GIGGALKNIGMGCGSRAGKMEMHSAGKPKVINDKCKGCFTCFKSCAQNAIYEIDGRAF
                     IDQELCVGCGRCIGSCLFDAITPASDETNTILGEKIAEYTYAVLKDKLHFHISFVIDI
                     SPYCDCHNFNDIPIVDDIGIFASFDPVAIDKACSDAVNNGRIIKESLLDKQEKKKDYI
                     TTVNPKTDWLHGIEYASSIGLGSLDYEIVNLDR"
BASE COUNT         5479 a         2170 c         2621 g         5058 t
ORIGIN      
        1 tatcagcttg caactgaggg atatgatatt gcgatattgg atgtgtttgc acaggaagat
       61 gtaaaagata atgtagaccg aattattaaa acaggagctg atgttcttta ttttcaggga
      121 gatctttcca aagatgaaag cagaaaacaa tttgttgaat ctgtagtaaa tcatttcggt
      181 agaattgatg tgttggtaaa taatgcagga gtaggtccta aggtaaggct cgatattctt
      241 gaaacaacag aggagagctt tgattttgta ttgggaataa atctcaaagg aacattcttt
      301 ttaactcaga ctgtcgctaa tgaaatgatt aaacttaaag ataaatttga agattgcaat
      361 ccgaaaattg taaacataag ctcaatgtcc gcatatacaa gttctacctc aagaggagaa
      421 tattgcatat cgaaagcagg tgtcagtatg gtaaccaagc tctttgcaga ccggttagca
      481 gaacatgaga ttaatgtata tgaaataagg ccagggataa tatatactcc aatgactgaa
      541 gttgtcagag caaaatatga caaattgatt gcagagggca tctgccctat aaaaagatgg
      601 ggatatccag aagacatagc taatgtagta tctgcattcg tatcaggtaa atttgcatat
      661 tctacaggag aagtaataaa tgttgatggt ggatttcaca tccaaagact ataatcggag
      721 gttagcatga atagaagcac tattaaaatt aataataaag aatatgattt cattactgta
      781 aatacggtaa ttgtaggaac tggagctgct ggttttaatg ctgcagacag tttatatgat
      841 ctgggacagg aggatatatg tattgtaact gaaggaagaa atatgggtac ttcaagaaat
      901 accggatcag acaaacagac ttactacaag cttaccttag ctggagatgg cgatgattca
      961 gttcacgaga tggcgaaaac actttttgca ggaggaagta tgcatggaga tattgcactg
     1021 actgaagccg ctttatcagc aaaagggttt tataaacttg cttctatagg agttccattt
     1081 cctcataata aatacggcga atatgttgga tataagacag atcatgatcc aagacagaga
     1141 gcaacatcag caggcccatt aacatcaaaa tttatgaccg aaaaacttga aaaacaaatc
     1201 gaaagtaaag gcataatgat ttttgatggc tattatgtaa ttaaaattct gcaaaaagat
     1261 ggttcagcat gtggattaat tacaattaac aattctaaaa cagatcagga taatttcgga
     1321 attacaattt ttaattgtac aaatattgtt tatgctactg gcggtcctgc aggtatatat
     1381 gatacaagtg tttatcctat aagccataca ggttcaacag gcattgcatt tgaagcaggt
     1441 gctaaaggca tcaatttaac tgaatctcaa tatggtattg cttcaataaa attcagatgg
     1501 aatctgtcag gaacgtatca gcaggtaatt ccaagatata tttctacaga tcaagaggga
     1561 aatgatgaaa aagaatttct tgatgagtac tttatagaca aaggaaaatt atttgactgt
     1621 atatttctta aggggtatca gtggccgttt gacccaagaa agattgaaaa tataggttca
     1681 tccattatcg atattttagt gtttaatgaa actgcaataa agggaagaag agtattcatg
     1741 gattttagaa gaaatccgaa acatttcagt tttgatttgc ttaatgagga atcatataca
     1801 tatcttaaga attccaatgc ttttcaaagc accccgattg aaagacttga acacatgaac
     1861 aagccggcta ttgaacttta tcagaacaat ggtattgatt tgtatacaga aaatcttgag
     1921 attgctgtat gcgctcaaca taacaatggt ggactaatgg gtaatatctg gtatgagagc
     1981 aacataaagc atttattccc agttggtgaa gttaatggta cttttggagt ttacagacca
     2041 ggaggcagtg ctttgaattc tactcaggta tcatccttca gagctgctca gtatatttct
     2101 cagaactata ctgataaccc gtcatcatct gaggaattca taatcaattg gaaagatgaa
     2161 attaatggtt gctatgaatt gacagaaaag cttatagata aaaagtcaaa ttctaatata
     2221 ctagacttaa gaaaacgcta tcagaagcta atgacaaaat caggtgctca tatacgctca
     2281 ctaaaggaag tttcagaagc tgtaaataca agtttgcagg agttaaaatc ttttgaaagt
     2341 gtaacaaaaa tcaataatac ctctcaaatt tcagatgcat ttaggaatag agatattctt
     2401 ataacacaat atgtatatct ttgtgcaatt aaggaattta ttgaaaaagg cggaagatca
     2461 agagggtcat atttagttag agatgacaaa ggatctctgc caatggaaga tctacctgaa
     2521 atttttagat ttaccttaga tgatcagcaa ttaatgaaaa aggcatgttc tgtatcattt
     2581 aacaaagaag acttatctgt aaaatatgaa tggagtgaag taagaccaat tccaactgaa
     2641 gacaactggt ttgagaacgt ctggtctgaa tacagaaaaa agaacattat aaaatagatt
     2701 tatttaatta atgatatata agtcttacca tcgtgtaaaa ggtcataagt aatatgtggc
     2761 ctttttacaa tttgtgcggg tttatatgaa ttttctaagt cattgatgaa ttcttttagt
     2821 tgtttatact gatgataaga aaaacttagg tcatcataat cgaaatatga atatacctga
     2881 ttccacatac gccatatgac attgagtccg taattataat acgttataga atcaggtata
     2941 gaagtacctg aaggaggttg aatttctgtg atgcatacta tttgcttttt atattctttt
     3001 gcaatattaa cctcattagc aatattttta atttccttat ttcttgcata attcataaca
     3061 gcaactttat cacaagcttc gctaatcaac ctttcaagga tatcataata attattatca
     3121 aaccagtatg gtatgcaaat tataacactt atgtcaaact gttttgcata ctcatatgta
     3181 tcaaccatat tcgatagaaa tttttccata actctatctg aatcttcttt ccattcagaa
     3241 agaagataag gctctacatc aaaaacaatt cctgaaaact gtgaattcgg atcaaccttt
     3301 ttattatact cataaataag atctattttt gcacgcatag atctgtttct tctttcagtt
     3361 gcccaagaag gatcacctga taaataaaat gtatcaatat tatttatctt catattttta
     3421 ataaaatcag aagcactagc atttactata gaattttctg aaaaagactg ataaatttga
     3481 tttattctat tcatttttaa taattctgct acagattcaa attctgatgg atccaattgc
     3541 gaagaactcc atgagaaaat agaaatatca taaaagaact gttgtggttt cacagcgtga
     3601 agtgaagata gtaataacgc aatggttatg tatattgcaa agattgttct cattttcacc
     3661 tcaaaaaata tcatgcttca cacatgatat tatatactaa taagatattc aaaaatacac
     3721 gaattacagt attaaggggt ttaagtaaaa aatttcttat aaaggcattg tatagctttt
     3781 tcaaaatcga tttcatcaat acctaccata atgcttaatt ttgaggaacc ctgatttagc
     3841 atttttataa ctatgttttc ttcagaaagc gcctctgaac acaaatgaag agtatgggaa
     3901 tcatctacac atttttcacc tactatcatt accagagctt gatgatgtat gattgaaact
     3961 tcctcagctt ttagttcatt tctaattctg caaatcagcc tgtcttcaat ctgctcgtta
     4021 aggtattttg atcttactat tattgagata tcatcaatac ctgaaggaga atgctcatat
     4081 ggtatcattt catcttcaag catttctaga atttttcttc caaaacctat ttcacggttc
     4141 ataagatatt ttcttacgct tatagttgaa aaacctttat caccgcttat accagttatt
     4201 ctgtgttttc tgttttcatt tacttctcgg tgtatcatag taccaggagc agatgggtta
     4261 ttggtatttt tgatatttac atgagtaaat ttgtaataaa ccggtgttaa tgcttcctca
     4321 tggaaaacgc tgaatcctgc atatgagagc tcacgcattt ctttaaatgt aagatggtct
     4381 ataggtaatg gattcttaac aatatttgga tttaccgtgt atatatagtc tacatcagta
     4441 aaattctcat aaagatcgca gtcaagtgct gctgcaagaa tagatcctgt aacatcagaa
     4501 cctcctcttg gcaatgtaac aacttcctga tttacagaat aaccaaaaaa accagggaaa
     4561 attactattc cttcataatc atttaatatt ttaagatttt catatgatac aggaagtatc
     4621 tgtgcattac caaattctga tgtgagaaga aggccagcat ctttcgggct gacatagtta
     4681 gctttatgac ctttgaagtt tacatactct gctactaatt ttgcacaatt atcttctccc
     4741 gctgctttta agttatcaag atatctttct tttacagatt tatcataatt gactctgtca
     4801 attaaatctg ctcttattat ttcaatgatt tgatcaggca tacctaaacc ttcggatata
     4861 tccttaaatc tttgtacaat tagatttaac tgattattaa tatcatcatg tgccaatgat
     4921 ttttccgcaa gttttattaa catatcagtt accttcgtat cttgtggata tcttttgcca
     4981 ggtgctgaaa ctaccacaaa ttttcttgaa gggtcagatg taacaatatc aaatactttt
     5041 tttacttgtg aaggatctgc aagacttgat cctccaaatt ttaccacttt taacattaac
     5101 ttctcctatg tgttctggta tttttactca gaacaatcat tcattagatt ttatttataa
     5161 ataaatgcat tgtcaaggtg tacaatcacg atgaaggaaa tatttatatt ttcataaagt
     5221 agtcatcttc tgaaatatcc tttttgggat ttacacatgt aatttcgtca aactcccaat
     5281 attttttaac atcattatta tttggaaatg taatagaaaa tatttcaaca tttttgcatt
     5341 caatagcaag gcgtataggt ctattttcaa atctgttgac acagtcatta tttttccatt
     5401 cgattttatg ataaatttca tcagtgttta tagctataca gtctttaaaa ttaaaccctt
     5461 caattggctg atttgtataa gcatctctta tacttgctct aagagtacct ttattgcagt
     5521 tagcattgat atacaactgt gacgaggaaa cgctaaatgg acgggttaga aggtatcctg
     5581 cttcatcaca tgcaatagat gtaaatccat ccggtctgag ttcggcaaaa ttaatatact
     5641 gaggtttttg agtaataaga tgagtattcc catgactgaa atctgatgct gcataataca
     5701 ttctgatttt ttctggaaga tatataagtg atgttagcgg atgtatcatc ccacattcgc
     5761 tggttttctc gtctttaagt gagataaacc gatttttagg attaagccat ctccaattat
     5821 atgatccttt actaaatgct gctgatatgt ccatggtacc ttccatttta gcccagttca
     5881 tatctgtttc gactgtatta tacaaagggg acaatccgat tacataatta ccataagaag
     5941 atgcttgtgt tccatatatc tgggtttggc atccttcaga tatatcaggt tcaagtataa
     6001 gttttggaaa gtcccaattt atgaaatctt tacttgaaga aatatagttt tttctctgtc
     6061 ccttaatatt tctaaataaa gcgtaatatg ttctttcaac aggatttttg tataatccta
     6121 ttttacaatc gctatcagcg ttaataacaa tgttaatatt ttttttatcc cagtgtatac
     6181 cgtcttttga aaagcatgtt gctattgcag ggatattttt gggcctcatt ataagcttat
     6241 atggcatatt tttatcttct ttgtcaatta ttacacttgc tcccatagga tacatatctt
     6301 tatccataac aacattagta ccatctatta catcaagaat tggcctatcc catttaattc
     6361 catccgaaga tgttgcataa catagatatt taatagcctc ttcatcagtt ttgtaaagat
     6421 tcgatgttcc ataccacatt ttaaaaattt tttcatcata atcataagaa actgtactat
     6481 atgaacagct gttatattca tactgattgt tgtttgcaac aaaaacagga ttatttttga
     6541 attttacggg tttatgcgtt ttcctagtaa aacctgataa agtttcatat gtccaataat
     6601 caaagaatag aaattttcta gcaatttgtt ttttcatgta agtctcctga ataaatattt
     6661 tcaacatttc atttcatttt acttgcggat tatatcatat atgagcagtt gtgccaaaag
     6721 tatcatacat atttattctt aattacattc aattatggta caatataaaa taactatagg
     6781 gggaagaagt atgaaaagaa gaataggtga caggtatgat ggatataaat taagaaatgt
     6841 tgacacattt tttcttctaa taccatacat catgaaaaaa cgtgatgaca gccagatttt
     6901 tttcgaggat gtagctgatg tagaccttct agatgcattt ataagaaagc atcaggagga
     6961 tattcctgga ttaaagttat atcatatttt tattgcaaca ttagtaaggt tgtttgctct
     7021 tagacctaga ataaacagat ttgtatgcgg cagcaaaatt tatgcgagaa aagaaatagc
     7081 aatttccatg tctataaaaa gaagtatgac agatgatgga gaagaaacta atattaagcc
     7141 tatatttgct cctgaatcaa cactgaagga catagttgat atagttaacg agcaggttaa
     7201 tatgaacaaa gaaactaatt ctgaaaacga tactgataaa ttcgccaaag ttgtagggca
     7261 tatgcctaca tggcttatta aagcagtagt taatactttg atgtggatgg acagaaaagg
     7321 tttaatgtca aaaaaaatta ttgatttctc tccatttcac tgcagcggat atctaacaaa
     7381 catgggctca ttaggtatta aggctgtata tcatcattta tataattttg gaacaacatc
     7441 tgtttttctt gctatgggga aaaaagaata tcagtatcag ggatctgatg aaggcgaatt
     7501 aataaaaaag aggataataa gtttaaaaat ggtagttgat gagagaattt gtgatggata
     7561 ttattatgca tcaacaatga gacttttctc taaaatactt caaaatcctg aagtacttct
     7621 catgcctcca gaggaaatac cggttgatga tggaattgta cgcgaaaaga aaaaaaaggt
     7681 gaagaaagca taaaataatg aaaagcaaac tactgaaaat tgctgctgta atgttatggc
     7741 taatagtatg gcaaattctt tcgatggtta taaataaaga acttctaatg ccatctcctt
     7801 atgttactat tagcactttg gtaaggctta tgtctgacag ttctttttat tatgcaatgc
     7861 tttattctct tttaaggata atttcaggat ttttggtagc agcagcatta gcttttattt
     7921 taggaattat tgctggtttg aatagaacgg ttgagatttt aatatctcct tttatcacaa
     7981 tagtccgatc tacaccagta atgagcttta taatcttagc aattatctgg ttcagttata
     8041 acctagctcc tttagttgta aatcttatta tgtgttttcc tccaatatat gataatgtag
     8101 taaaaggtat caagaatact gataggaaac ttctggatat ggctaaagtt tataaagtca
     8161 aaccttttaa tgtcttcaaa gggatatatt taggttcgct tatgccattt ttatctgcag
     8221 gtatgaacac tgcgttaggt cttgctttta gagttgcagt tgcagcagaa gtattagcaa
     8281 atccaagata tggtatagga actagactgt tacaggcaaa atataatctg gaaactgcag
     8341 aggtatttgc atggactttc atgattttac tattaagtta catatttgag tatattttta
     8401 aactaataat tgaaggtggg aggcattatg ctcaaaataa gaaatctgac taaatcgtat
     8461 aacggtaatc agatattcag aaactttgat cttgatatag aaaagaacag aattacatgc
     8521 cttttgggaa agtcaggtag tggcaaaaca acactactta atgtattatc ttctattacc
     8581 gatgaatata ctgtagacta caatgatttt aaaaatttaa catattcgta tatatttcaa
     8641 gaacccagat tgctaaattg gcatactgta tttaaaaata tgttatttgt tctggatgga
     8701 aaagaagcaa aaagcaaaga agcacataat aagataatga attacttaaa aatggtggaa
     8761 atggataagt acaaagacta ttatcctgat cagttaagtg gaggtatggc tcaaagagta
     8821 tctattgcca gagcttttat taaggatttt gatgttcttt taatggatga gccatttaaa
     8881 gggttagatc ctactcaaaa agaagatatt atggatgaat ttttaaatat gtggaaagat
     8941 aacagaaaaa ctgttctgta tgttacacat gaattggacg aagcattata tttggcatat
     9001 gatatatatg tgtttggtgg aaagccagca aaaatagttt atacatgcaa aaatgcatat
     9061 aagatgacag ataaagaaag agaaatagaa agggaaaaaa tatggcagat attaaaatta
     9121 cagatttaac tgtaggtatg gaaataaaaa atgaactata tattctttgg tataacgagc
     9181 ttaaaaaagc atcaaataat aagttctata tggattttca gttcagagat aaaacagggg
     9241 agataaacgg taagaaatgg gatgcaactc caaacgaact gaatgattat aataatgcag
     9301 agttgtattt tgttacagga agggttactg aatttcaagg ctctttacaa ctaaatgtat
     9361 cttctttacg taaagctcca caacaaatac agaaaaatat agctgacttt gtacaaagcg
     9421 caccagtgtc atccgatgat atggtgaata aagtttatga atttataaac ttgattaaag
     9481 atgaagaatt aaaaaaactc tgtacacaag tgtatcaaga aaatattgaa caaataagat
     9541 tttatcctgc tgcaaaagct cttcatcact ctgtaagagg aggctatctg tatcacatta
     9601 ctacgatgct tgatcttgct aaagccattt caaatgtata taccacagta aatactgatt
     9661 ttctatactc aggaatattg ctccatgaca taatgaaact taaagaaatg aatgcaaatg
     9721 aatgtggaat tgctgattat tcaactgagg gtcagttgat gggacatata gaaatgggaa
     9781 tatgttatat aggtgaaatg gctaaaaaag taggattatc gcagaataag gaacttttaa
     9841 ttaaacacat gatattaagc catcattata taccagagca tggaagcaca aaaatgccta
     9901 tgtttttaga agcagaatta ttacatcata tagaccttat tgatgcaaga acgtatgatt
     9961 tcaataaagc atacgagact cttcaaaaag gtgaaataac agaaagaact gttttttcac
    10021 ttgatcgaaa agttatgaat cctgattttg agaaataaaa aagacataag atataatctt
    10081 atgtcccatg gtgcgccatc gggggctcga accccggaca ccctgattaa gagtcaggtg
    10141 ctctaccagc tgagctaatg gcacaagcgc aactagtatt ttaagtttta atttatacca
    10201 tgtcaacagt aaatttgctt tttgacaagg ttttttggct gatatatcat tatcatgtac
    10261 ttattcatag gagttgttat gaaagatata ctgttagttg acatgaatgc tttctttata
    10321 atgtgtgaat caagaaggga caattcgctt cttaatactc cagctgctgt tgccggtgat
    10381 cctaaataca gaactggaat aattttagca gcaaattatg aagcaagaaa atttaatgta
    10441 aaagttacaa tgacagtaaa tgaagcactt aaaatatgtc caaatctcaa attagtaaaa
    10501 cctgatcatg aatattatga gaagtgctct aaacaagtat ttagtttatt gtctgaatat
    10561 tcacctcaac ttgagattaa cagtattgat gaagcatgga ttgatgtgac atcaagtaaa
    10621 aaactctttg gagatcctta tgaaatcgca tgtgatatcc aaaaaagaat taaagaggaa
    10681 cttggaatat ggtgttcaat aggtataagc gaaaataagt ttttagcaaa aatggcgtca
    10741 gaaatgaaaa aaccattggg aattacgaag ttatacaaat catctgtaag agaaatgtta
    10801 tggcctctat caataaataa attatatgga attggaaatg caacagctaa aaagcttaac
    10861 aaagatggta taactactat aggggattta gcaaaagcag atttatcata tctcgtatca
    10921 agatttggta aatacggtgt ttacttatat gaaagatcaa agggtattga tgatgattta
    10981 gtatgcccga atgactcaaa aagtttatca tgtggaagaa tggaaacatt gacacatgat
    11041 attacagatt taaatgaggc aaaaagagta atagcagttt tatcagagtc aattgcacgt
    11101 agtgtaagac agaaaaatct tgtgggaagt gttgtacaga tacaaatcaa atataataca
    11161 tttaaaatga taagcagaca gattactata gaagaaacca gtctgacaaa agacatatac
    11221 aatgctgggt gttcaatctt agaaaataat tgggaccaag acaagccggt aagattatta
    11281 ggtatttcac tttgctctct taagcaaaaa gacatgcaga taagttttga tgttaatgga
    11341 aaacaaaagg aggaacattt ggaaaaaaca attgataaat taaatagtaa atttggacaa
    11401 gacataataa agaggggaaa tactatatga aaattgtctt gtttgcttta aacagctcat
    11461 ttgttcatac taatctcgca ataaggtgtt taaaagatta cattgataaa aattcactta
    11521 aaaaacatga tgtacttctg gttgaaaaaa atcataaaga taagtttgat gagatattgc
    11581 attctttaca tgaatgtaat gctgatttat atggattttc atgttacata ttcaatattg
    11641 atgaaataat acagatagca aagtcattaa aaaaactttt accaaacnnn nnnnnnnnnn
    11701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11761 nnnnnnnnnn nnnnnnnnnn nnnnnnntaa ttgctcatac tgtttatctt caactacaaa
    11821 aggtatacgt tcgaaaactt ctcaaaagac tcttgatgat ttatatgaat ttgagaaatt
    11881 cggttctaaa attaaaatta taaaatttgt agatagaaca tttaattttg atattgaaag
    11941 agccaagatt attttaaatg gtttaacaga tattaaatat tcgaagatat atcacatgga
    12001 aatgagaccg gaattatttg acgatgaaat gtttgaaata ttggcaagat tcccaaaaga
    12061 caaattgcaa ttagagatcg gtattcaaag tacaaataaa aaaacattag aagcaataga
    12121 ccgaccttct gatattaata aagcaatatc aaacatgaaa agaataagga ttagtaataa
    12181 tatcaaaatt cattgtgatc ttattgcagg tcttcctttt gataatatta atactattag
    12241 acaatcatat aatgatgtat tctttgcttg tgacgagctt caactgggtt ttctgaaaat
    12301 gcttaaaggt tcaaaaataa gaaaacaagc tgacaaattc aattatatat acaatgataa
    12361 agctccatat gaagttttat caaatgactg cataagctat gaaatgatgt ttaaattaaa
    12421 aagaatagct gctttgactc aaagattcaa gaacagtatg catttcaaaa aatcatgtga
    12481 gtatatcgta tcttttaaca taagtccgtt tgactatttt tatggattta atgaatatat
    12541 cggtgaacat atcagtcact tatcacagca tgaagcattt aagaagtttt ttgagtattc
    12601 atcttttaat attaaaaatt ccatttcagt cgatgaatgg gataaattta taaaaatatt
    12661 atgtgaggaa tataaacaat gcttaaataa aaacgcacct gagtatttct gttaatcatc
    12721 aattattttt cttgcttcaa aaatgaaatt tttacttgat tctatatcag aaacataagc
    12781 ataagcactt aattttccta gattagctat gtttttaaaa aaagtatctt tatttattgg
    12841 aggtaatata ataccacaat cctgagattg ggaaatgttg tcatagaact cattagcatt
    12901 agaataaaag catatatcat tatttctttt gacaagatat tcgttaacga catcgagaat
    12961 attaataata cctatatcag aatctggatt tcgaaatgta ataaggtaga atgattcgat
    13021 atcggtcatt ccataacata cagatttgta tttgtcgaat cttttattga ttgcgttatt
    13081 taattctttt aaggagattt gagattcaac ttttatcttg gtattatttc tgatttgctt
    13141 aacaagatca tcaatttgag tatttttaag tattatataa aaagggttca ttattgtact
    13201 attgttactg atattgagta gttcaaccat ttggaacctt gacggatgta aagaatataa
    13261 atcgtttcca tatttatttt taagccattc ccagtgaatc ttagcacttt cgaaggactc
    13321 aatgccatcg gatattatga acatgggctt attttctatt attacctttt ccttaatttt
    13381 cattaaagca ttgcttatta aagtgtagtg ctggggagtg gaaatctgat aaccttcata
    13441 tactccgtaa attggatggt caaatgagaa aatcaactta aaattgaaag tacaagaaat
    13501 taaaggttca atcaatgaaa aagatttatc ctcgataaaa gcttgtgaga aaggaaaatc
    13561 aataactgaa tcatttaaaa attcagaatg tctctttgct ttatcttcat cgaaatcttt
    13621 agcataggat attattgata aaggctggtt actatattgt tcaagatcaa gtagaacaag
    13681 aagaccatat ctttcttctc ccgaagggta gatgtgtcga gtaaggacaa aaccttcata
    13741 atcagataaa aaaccagacc tcaaagatct tttaacattc ttatttattt tgttaaaatg
    13801 attttttgtt aattttacat tatcactatt atattgtgga gtataatctt tagaattcat
    13861 taggaaaccg atatcaagcg gatctattag cttctcatta agattattat atgtaaaagg
    13921 aaaagaaggc cacaggatat ggtcacttgg attatctgga atatataata ttggagcatg
    13981 tacagcacag tctaaaagcc ttttttcaat aaaatccatt attgtatccc tttgtgaaat
    14041 aaatatatca taaacaatag tcaaaatcca actaattatt aaaagttacc tttaaaattt
    14101 gcattttttg tttattattt gcaaatcact tattttaaaa ttaataaaac attattaatt
    14161 gattttattc ataatcaaat tgaattaaac actattaaaa tatataatag tatataagat
    14221 agaaatacgg aggaacaatg gcgtcaaaag tatattttac aaatatgaaa acagatcacg
    14281 gacaaagtct cttgcaaaag tttgagaaac ttataaaaaa agcaggatta tcagagattc
    14341 cttttgaaaa taaatttaca gctgtaaagg tgcattttgg agaaccaggt aatttagcat
    14401 atattagacc gaattatgcg aaagtattat gcgattatat aaagcattta aaaggaaggc
    14461 ccttcttaac agattgtaac accttatatc ctggtagaag aaaaaatgca cttgagcatc
    14521 tagatacagc atatcaaaat ggttttaatc catttgtaac aggatgcaat gtaattattg
    14581 gagatgggct taagggaact gatgaaactt taatagatat aaacatgaaa catgtgaagc
    14641 aagcgaagat aggccatgct gttgcagatt cagacgtaat aatatccctt aatcatttta
    14701 aagggcatgt tgcaactgga ataggcggtg ctttaaaaaa tatcggaatg ggttgtggtt
    14761 caagagcagg gaagatggaa atgcactcgg caggtaaacc taaagtaatc aatgataaat
    14821 gcaaaggatg cttcacttgt tttaaaagct gtgcgcaaaa tgcaatttat gagattgatg
    14881 gtagagcatt tattgatcag gaattatgtg ttggttgtgg aagatgtata ggctcttgtc
    14941 tttttgatgc cattacacct gcaagtgatg aaactaatac tatattagga gaaaagattg
    15001 cagaatacac atatgctgtt ttaaaagata agcttcactt tcacatatct tttgttattg
    15061 atatatcacc atattgtgat tgccacaatt ttaatgatat accaattgtt gacgatattg
    15121 gaatatttgc ttcgtttgat ccagttgcaa ttgacaaagc ttgttctgat gcggtaaata
    15181 atggaagaat aatcaaagaa agtcttttgg ataagcaaga gaagaaaaag gactacataa
    15241 caacagtaaa tccgaaaact gattggcttc acggcatcga atatgcaagt tcaatcggtc
    15301 ttggaagttt ggattatgaa atagtaaacc ttgacagata aggtttgcat tttaagaaga
    15361 ttgaaacaga tgtgcagtta aaatttaatt gcatattatg gttatttatg ttacaatact
    15421 tcagatta
//
LOCUS       DAOSXM010000050        15318 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2111
            NODE_2424_length_15318_cov_9.861168, whole genome shotgun sequence.
ACCESSION   DAOSXM010000050 DAOSXM010000000
VERSION     DAOSXM010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864178
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 15318)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15318)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:24:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,261
            CDSs (total)                      :: 2,229
            Genes (coding)                    :: 2,218
            CDSs (with protein)               :: 2,218
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 3 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15318
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2424_length_15318_cov_9.861168"
                     /isolate="AMR_MDS_2111"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            411..1049
                     /locus_tag="PLQ68_07220"
     CDS             411..1049
                     /locus_tag="PLQ68_07220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72480.1"
                     /translation="MWFGDLSNSGAADLAQQAIRSTDNFNWTFIALFAFVVYVYATEL
                     HKKNYRFVIAGLSLYMVHWFYEIMNAIICTATGYALWTVSPESTSFMLLIGVSWELSM
                     MFAIAGLIMSKLLPEDPKKKILGINNRILFAVGNAAFFSAVEIFLASTPAFIWVYPWW
                     GTIPVFITTYIPFFLAAFLVPDRSPKFQKIFIGGLAAVNILLLAVLIPLGII"
     gene            complement(1490..2375)
                     /locus_tag="PLQ68_07225"
                     /pseudo
     CDS             complement(1490..2375)
                     /locus_tag="PLQ68_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004802010.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
     gene            complement(2468..2680)
                     /locus_tag="PLQ68_07230"
     CDS             complement(2468..2680)
                     /locus_tag="PLQ68_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012859401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HXK72481.1"
                     /translation="MKVSYDGLWKILIDKYMNKKNLTKICKLCPATISNIGRGEFVSM
                     DVLYCIGTKLNVNFGEIVSIIRESKD"
     gene            complement(3291..4835)
                     /locus_tag="PLQ68_07235"
     CDS             complement(3291..4835)
                     /locus_tag="PLQ68_07235"
                     /inference="COORDINATES: protein motif:HMM:NF013400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72482.1"
                     /translation="MKSIQIDRYAKTIPTDYSWQMGIGNDHACQMLRTDVCQHVKLAH
                     DELGIQYIRFHGIFDDDLLVIQRLTDYHNFKAMPYAKGIEETSFRQIANVFDNVLKCG
                     MKPFVELSFMPTAIASGKRVGLRYKNNITMPKFTERWCELIKKFILFLFDRYGKDEIR
                     TWYFEVWNEPDLSIFFKGNQKDYFRLYKATSDTIKKIDSNLRVGGPATSACLWISDFM
                     KYCDENKVAYDFVSTHHYPGDAFGNMFTLKDAFKMMKTTHENSKNKINLSETLREYFF
                     KPETYKTWTKGILREMDEKVRHTVQDKPLFMTEWNSMAVYTSPVHDEKYSAAFLVKSI
                     LDLKGIMDGYMFWCCSDVFEEMFILGKPFHGSYGIVNNDGIPKPNFWGFKMLSQLYPN
                     RLDFPITNEDIEYAVFTDMDKTQILLYAQDFDYYKNDVFDIEISINCQTQNVYKQVID
                     SMHCNPKAEWTKLGSPDLLSLGQVAEITEKTKLKNEIQPFYTENGETIIKLQIHTNDV
                     VMLTME"
     gene            complement(4832..7387)
                     /locus_tag="PLQ68_07240"
     CDS             complement(4832..7387)
                     /locus_tag="PLQ68_07240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002605000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HXK72483.1"
                     /translation="MLKIDEIRIENLIEGCITDNCNPVFSFSLLSEKQDTYLVKAVVK
                     VGDWQQTVYSQKDIIYTGVLIPFTMYIVDITAYDNHGEIAVKTAYFQTGRMKTIWDAK
                     WITKTSYTPEKKKSPIPMTFEKKFKSSKQIKRAYMTVTAMGIFEINLNGNVLTQEYFA
                     PGFTSYKHTLQYVFYDITDYINLENKLIAVVGGGWAVGRFTYSSKSQITTKRQMLLAE
                     MFFEYADGSFEKIVTDETWKVTLNGNYRFGDFYDGETFDSTIKLDSVSWESADTAKLK
                     FKPKITARYGCPVKAHETLLPVNVFVAINGEVVYDFGQNFAGVIHLKINGRENQKIVI
                     RHAEILCKGNLSVKSLRTAKATVTYICSQGEQIYSPKLTYMGFRYVGIRGIERKDINV
                     SAFAIYSDIEAIGAFECSNPLINKLQSNIVWSSKSNFVDIPTDCPQRDERQGWTGDIS
                     VFSRTACYNFDLSRFLGKWLADVRYEQGKLGGIPFIVPRHGDIWPVIPTACWSDCCIL
                     VPWAEYLARGSKTLLKKQYPVMKKYLKAVKFWASLFSFSKDQKKIWKYLFQFGDWCAP
                     DGYIKDWMKKGKWIATAYYANSCNIMAKISDILGYAKDKEYYNKLSTEICTAYINVFT
                     NGDGKLDNEFQTAYVLPIYFNMTDESTKLRMANNLERLVKERDYHLSTGFTGTPYLLF
                     ALSDNNKVDVAYKLLMQDTCPSWIYQVKHGATTFWEQWCAVSPDNEEKGLTYDESDSE
                     ASFNHYAYGAVGDFLYRRIAGIEATSGGYETFSIKPVIGGNLIYAKSSQKTPYGTITS
                     KWEISNDIFSIDFTVPVSTKCTITLPSGKTANFGSGKYTLSEKINNELQRNEL"
     gene            complement(7412..8866)
                     /locus_tag="PLQ68_07245"
     CDS             complement(7412..8866)
                     /locus_tag="PLQ68_07245"
                     /inference="COORDINATES: protein motif:HMM:NF016497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HXK72484.1"
                     /translation="MCNPFLPLNEYIPDGEPHVFGDRVYLYGSHDKQGGKRFCELDYV
                     VYSAEINNLEHWDYHGISYKKSQDPRSTESGFADYYAPDCVKGNDGRYYLYYVAMGPN
                     TRNFGPIAVAVSDNPEGPFEYLGDIQYPDGNPVLKYLTNDPAVINDDGRIYLYYGWGL
                     GRDFRSKLLTPLYNTVQSKLYSRSVKEIKSTKPNILSCAFAELENDMLTVKSEPISVL
                     DSKTTADKKSELYNHAFYEAPSIRKFNNTYYLIYSSGENNELAYAMSKYPNKGFEYKG
                     VLISNSDLGYLGNKNRLAPAGTIHGSIEYINSDYYIFYHRCTHNTDFSRQACMERIMM
                     NSDGTFDQVEVTTQGSGNSLNKGKYPAAVCCNLYNKKLKKVQGNNHEQGQPNITHGIE
                     GQYITAISNGTVIGYKYFDFQQVKRISVTVRKANGYFEISIKPGKVISVIQLNYSDDW
                     QIHKQDISIEDGIKPLYFTYRGKKKAQFLNFEIE"
     gene            complement(8887..10353)
                     /locus_tag="PLQ68_07250"
     CDS             complement(8887..10353)
                     /locus_tag="PLQ68_07250"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXK72485.1"
                     /translation="MGNNLRTKKSYPADFKTMFGLSTMNWTETVSGAFMTGLFMLFLT
                     DYANIGAYAATLGTMLLIFGRIVDAVDDPLQGYIMDRAKPGKLGKYKPFIILSTILTT
                     LALCFIFGLPVSIAQNPVLLTIWVAFFYLMYDVGVSFFAENPLRQSLTTDPVLRSRMT
                     TWPRVISMIIVIPMSFFISIMTTINKSVGDMSRSFSILAIAFMVFAGIISLIGISFVK
                     EGKHMEEEESQKISFKDIGKMLKTNKPLLISMLVSIFHGFVWTLVFATTTYYIKWAYC
                     TDLTTGVVDQARFGTLTMILGIFQLLPSIIAAAISPKLVKLFKGPVKVYKLSMWLELA
                     GGLGLFIFMLSGLLVKTPAIFFIMVLVILFGGGLSFVPGTLIGIESMDYGMYKTGKEM
                     HGIINSIGRFIGKAQTALSAALVGGVLIAIGYQVDSVTDTFLGDLSTIPQMLNSFIII
                     SGLVPAILCIISLFILKFYPIDENLRREMNEAINNMKE"
     gene            complement(10394..12871)
                     /locus_tag="PLQ68_07255"
     CDS             complement(10394..12871)
                     /locus_tag="PLQ68_07255"
                     /inference="COORDINATES: protein motif:HMM:NF014026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HXK72486.1"
                     /translation="MAKFKLFGLRTDDESQREKQHRQLCREVAGEGIVLLKNEGVLPL
                     RNKKIALFGAGARMTVKGGTGSGDMQERYSVSIEQGLRNAGFEIVSTRWLDRFDQYYA
                     QKDKIWRQSIEEKIKTYKPWQVQKMFDEVIHVNPLCFPCGDKIQIEDLSANTDTAIYV
                     VARQAGESADRRFENGDFKLSEEESYNIHSLAEQYEKLLLVINCGGILDLSILNETNN
                     IGAVLYYVQGGEEGGNAFADIVSGKINPSGKITDTWAKRYEDYPSAMDYGYLNGNLKD
                     EDYKEGIYVGYRYFDSFGINPLFEFGFGLSYTTFSHHVSDIHIQKTKVTIKVMITNTG
                     TEFSGKQVLQLYLEKPNGRLCHELRSLVAFVKTKNLKPDENELLTLTFDLCDHASYDE
                     KDSNWVLDQGEYGICLGTSSRQNEPVAVVVLENETVVEKCTSICPMEYDFSELKAEFP
                     IHEYDLNLPRFEVRSDDISYVKHVYENPKIKTNLKTEQLLLNLNDKELIELIVGAGYS
                     GKSFNNTPGCAGRTTVSLLKKSIPNVNFSDGPAGLNLTPKIVILKSGSMRYIDGIPAM
                     WKWGWLKKIEPFVKAKAGRGTPVYQFMTAWPAVTLQAQTWNLPLIEKVGNAVGVEMIE
                     TGVTLWLAPGMNIHRNPLCGRNFEYYSEDPILTGMMSSAVSKGVQSHKGIGVTVKHFC
                     CNNQEDNREYVSANIKERALREIYLKGFRYAVEHSNPKAVMSSYNLVNGVYTANSFDL
                     LTKVLRCEWGYDGMTMTDWNSTESDKGKHELCPVCGTDLIMPGSKAAKKALLKGLKND
                     SINRDDILRCAANVLNMIFESAVIEGF"
     gene            complement(12873..15212)
                     /locus_tag="PLQ68_07260"
     CDS             complement(12873..15212)
                     /locus_tag="PLQ68_07260"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HXK72487.1"
                     /translation="MLFCLNENWSFRKEGDSSKKVNLPHDAMQTEKRDPYCRNNKQSG
                     FFPGGKYIYEKHFNLDKSDIGKCIEIHFKGVYQNSDVVVNNVKFTGRKYGFVPFTIDI
                     SDAVRAGENTLIVNVDNSLEPNCRWYSGSGIYRSVQLEIRDKCHIKRVNIETLSINPA
                     VVRIIAETTLNCEIAVDIYDNEKMIISGNIGEFTIPNPKLWSAEHPFLYTCLIRTNTD
                     ERKILFGIRKLEWSANTGLLINNEEVLLRGGCIHQDNGVLGACIYPDSEERKVRILKE
                     AGYNAIRCAHNPASFELLNACDKLGMYVMDEAFDMWYIPKNYHDYARYFQTEWKKDIF
                     TMVENCKNHPSVIMYSIGNEVSETATKKGVETCKQLADYVRSLDSTRPVTAGINVLLN
                     VYARAGFGVYKDKGDYKAKPLEPVNKKIKEKKTGSAFFNSMIQKLGPLMFLVSKGRKG
                     DKASKGAAKSLDILGLNYAASRYDYDVVKYPERMIVGAETIIKELPYNWERVKKHKAI
                     IGDFAWAAWDYLGEACIGDWTYHSYKGLPLLAGSGAIDITGKIGAEAYFQQVVWGLRT
                     KPFIGIHPLNHAHEIPSKSAWRFTDCIDSWNWQGYEGVQAIVEIYSNSEFVRLSLNGK
                     EIGTKKVKKFRTLFKTKYQNGILMAEALDSNKKVISVHSLVSGSIETILSVRPDKRSI
                     YANGIDLCFIEIEFTDKGGNIKPFIEEKVELEITGPAVLAGFGSALAKTDEVFDKQYH
                     NSYRGRTIAVLRAGYKTGKTIIEVKSSGVQSVTLEIEVQ"
BASE COUNT         4690 a         3072 c         2225 g         5331 t
ORIGIN      
        1 catcttttac atttatctaa aaatgccgct tgacatttta tagctggtct aaatatttca
       61 ttacctctat tctaatattt tgacctgtct actgcaacag ttaacaatag gatgtatgat
      121 aaaaactgca tccatacata aaaaagatat acagcacaaa tagaaaagaa aagtagatta
      181 atttgttatg atgaaaagat atgtatatat acttaaagtt tgaatatcca ttatatgata
      241 agcgaataat gacaataaaa cattacatta actactgaat ttccgaaaga ttcaaaaaat
      301 cttgaacaga ttaagttgat ggcatacaat gtacctaaca ggtgtcaaat atataaattt
      361 attaattgta tgaaatgaga tataaataaa aatgaaagga agtaatattt atgtggtttg
      421 gagatttaag taattcagga gcggccgacc ttgcacaaca agcgattcgg agtaccgaca
      481 attttaactg gacatttatc gcactttttg catttgtggt atatgtatat gccacagaac
      541 tgcacaaaaa aaattatcga ttcgtaattg ctggattgtc tttgtacatg gtgcattggt
      601 tttatgaaat tatgaatgcc ataatctgta ccgcaacggg atacgcgctt tggacagtct
      661 caccagagag taccagtttt atgcttctca tcggggtgag ctgggagttg tccatgatgt
      721 ttgccatcgc agggcttata atgagcaaat tgctgccaga ggatcctaag aagaaaatat
      781 taggaatcaa caaccgtatt ctatttgcag ttggaaacgc ggcatttttc agcgcagtgg
      841 aaatatttct tgcttccacc ccggcattta tctgggtgta tccatggtgg ggtacaatac
      901 cggtattcat tacgacatat attccgtttt tccttgcagc atttcttgtt cctgacagaa
      961 gtcctaagtt tcagaaaatc tttatcggtg gcttagcagc agtcaacata ctgcttctag
     1021 cagtacttat accattaggg attatataaa taagaaggat aatctttatc ctgctcactt
     1081 tgatgtttat taaatataaa agtgctcgta aatcctcttt cttaaggttt ccggacgttt
     1141 ttatactgca atttattaca tcaaatcatc tattttgttt ctactggtac agttttttca
     1201 tatcttttga tatgtttatt aatatttgtg tatcattcaa agtataatgt acccagcttc
     1261 aagaccactg ctctgttaaa tactgaaaag aattgaagtt aacgttagca ttattgaaat
     1321 aaataaaaaa acgctaatgt taaacttgaa tttagtactt tttttgatag tacatactca
     1381 taggaataat aggtggaatg atgaactggt atccaagaga gagctactta aaaaatccgt
     1441 gatttttatc atgcaactga catctgattt tcgataaatt agccatactc tacctcactt
     1501 tatttattag actttgaatt aaatctttat cttctatctt agtaatacca aaactctttt
     1561 tacctgcatc tttgatagaa gctccaatat gataaacccc tgttttgtct ataattaaaa
     1621 atctatcgtg aaaatccgta gtagttttaa cagatagttt tggatactga gcgttaaatt
     1681 tagttatatc ttttaccgat aagcttccct taccggcagt taatatattg atatctacgc
     1741 ctttgttttt cttacatagc atattaagcg tatttacatc tacatagtta tcaatcaaaa
     1801 ttagctcttt atttgctttt tgaataatgc taacaatgaa tctgaatgca tcatagattt
     1861 gaccatcaaa aaaaattttt tgttttacct ctgtgttatt agtcatataa ttaaatactt
     1921 cctcaagctt cctatctgtt tctaattgct taagctcaac cctatccagt cttgaaaaca
     1981 gttcttgatt tgaaagtaaa aattttctca tttctacgaa gctattcata attttcacac
     2041 tgacttcaac tgcaacatca cttttaagca caacagaaag catagcaatg ccttgttctg
     2101 taaacgcata aggatttgat gttgaatgtt tcagtttttc gaaccggtcg caatttgcga
     2161 ccagttcatt ttttttcttc attgctaagc ctaaaacaaa attcttcagg aaatctacct
     2221 aaatttctct tgacctgttc attaagcctt ttagtttgga cttggtataa atcggctaaa
     2281 tctctgtcta ccatcacttg cttacctcta aaggtataaa tcatgtttct tatatcttta
     2341 tttctatcca tgacaagatc cttatcttcc accataaata cctccacttt ttcacttgga
     2401 ctggtcatag attgtaacca aataattcta atctaacttg aggtaatatt ttgtcacctc
     2461 aagactatta gtctttagat tctcttataa ttgaaacaat ttctccaaaa ttcacattta
     2521 gttttgttcc tatgcaatac aaaacatcca tgctaacaaa ctcacctctc ccgatattag
     2581 atatggttgc aggacataat ttgcaaatct tcgttaaatt ctttttattc atatatttat
     2641 ctattaaaat tttccacaat ccatcataac tgactttcat atatttacac cttaattaca
     2701 agtttatgaa attatttcat agatattgta gtttttttgt catcattttt aaagtgacaa
     2761 atattgaaac tgattataaa tcaatcctgt ttgatttggt ctctatgcaa tctgatttaa
     2821 gttatttgta gcattggata gtaaaccttg caaatttgcg aactacatcg gcaattgaat
     2881 aaccgagtat tcctgcttaa tatcctaaaa aacgcaaata caaacaaaaa gactgtaaca
     2941 actaagctac agtctaattt cttatttcca tttgtaatct actggaagat attattgaat
     3001 ttattctttt tgaaatcttt attccatctt gtcccagttg ctcatttcaa caagctataa
     3061 tgcttgattt taagccattt tcgaagaaat atactttatt cccactcaat agttgccagt
     3121 ggctgtatgg ttatttcgta gactattcat actaacatgc tttggggata tgaacatatt
     3181 ttgatgtctg actcttaaaa aagctctttc tttctttctt gctacttgat atataactta
     3241 tcctaatata ttaatgctac ttgcttattt caacaataga aatgatatat ttattccatt
     3301 gtgagcataa caacatcatt agtatgaatc tgaagtttta ttattgtttc tccgttctct
     3361 gtataaaaag gttgtatttc atttttaagt tttgtttttt cagttatttc tgcaacttgc
     3421 ccgagagaaa gcaaatcggg acttccaagt ttagtccatt cagcttttgg attgcagtgc
     3481 atactatcaa taacctgttt atacacattc tgagtttggc agttaattga gatttctata
     3541 tcaaacacat cgtttttata ataatcgaaa tcctgagcat atagaagtat ctgcgtttta
     3601 tccatatcgg taaataccgc atattctata tcttcattgg ttataggaaa gtcaagtcgg
     3661 ttaggataca gctgtgacag cattttaaaa ccccaaaaat tcggttttgg tattccatca
     3721 ttgttaacaa taccataact gccatggaaa ggctttccta atataaacat ttcttcaaat
     3781 acatctgaac agcaccagaa catgtaccca tccataattc cttttaagtc aagaatgctc
     3841 tttacaagaa acgcagctga atatttctca tcatgcactg gtgaggtata aacagccata
     3901 ctattccatt cagtcatgaa aagcggttta tcctgtacag tatgtcgaac cttttcatcc
     3961 atttcacgta aaataccttt tgtccatgtc ttataagttt ctggcttgaa aaagtactct
     4021 ctgagcgttt cactaagatt aattttattt ttagaattct catgtgtggt tttcatcatt
     4081 ttaaatgcat cttttaatgt aaacatatta ccgaatgcat ctcctggata atgatgagta
     4141 gaaacaaaat cgtaagctac tttattctcg tcacagtatt tcataaagtc actaatccac
     4201 agacaagcgc ttgttgcagg tcccccgaca cgaagattac tgtcaatctt cttaatagta
     4261 tctgatgttg ccttataaag cctgaaatag tctttctgat tacctttaaa gaatatcgaa
     4321 aggtctggct cattccaaac ttcaaaatac catgttctaa tctcatcttt cccataacgg
     4381 tcaaaaagga ataaaataaa tttcttaatt agctcacacc atctttccgt gaattttggc
     4441 atagtgatat tgtttttgta cctaagacct actcttttcc cacttgcaat agctgtaggc
     4501 ataaatgaaa gctccacaaa aggtttcatc ccgcatttca atacattatc aaatacatta
     4561 gcaatttgtc tgaagcttgt ttcttctata ccttttgcat aaggcattgc tttaaaatta
     4621 tgataatcgg tcagtctttg tatgacaaga agatcatcat cgaatatccc atgaaaacgt
     4681 atatattgta tcccaagttc atcatgagca agtttgacat gctgacacac atcagttctc
     4741 agcatttgac atgcatgatc atttcctatt cccatctgcc aggaataatc tgttggtata
     4801 gttttggcat atctgtctat ttgtatactc ttcataattc attcctctgc aattcattat
     4861 ttattttttc agataaggta tatttcccgc ttccaaaatt tgcagtttta ccgcttggaa
     4921 gagttatagt acattttgtt gaaacaggaa ccgtaaaatc tatactgaaa atatcatttg
     4981 aaatttccca ttttgaagta atagtaccat atggtgtttt ttgactgctc tttgcatata
     5041 tcaaattacc tccaattaca ggttttatgc taaatgtctc atatcctcct gatgtagctt
     5101 caatccccgc tatcctcctg tatagaaaat ctcctactgc accataagca taatgattga
     5161 atgatgcttc actgtcagat tcatcatacg ttaatccttt ttcttcatta tcaggactta
     5221 ctgcacacca ttgttcccag aatgttgttg cgccatgttt cacctgatat atccagcttg
     5281 gacatgtgtc ctgcataaga agcttatatg ctacatcaac tttattgtta tctgaaagtg
     5341 caaatagcaa ataaggagtt cctgtaaatc ctgtgctcag atgataatct ctttctttta
     5401 cgagtctttc tagattattt gccattctca attttgtaga ttcgtctgtc atattgaaat
     5461 atataggcag cacataagca gtctgaaatt cattatccag ttttccatca ccatttgtaa
     5521 atacattaat atatgcagtg catatttcag ttgaaagttt attataatat tctttatcct
     5581 tagcatatcc taaaatatca gaaatttttg ccattatatt acacgaattc gcataatatg
     5641 ctgttgcaat ccatttacct tttttcatcc agtcctttat gtatccgtca ggagcgcacc
     5701 agtcaccaaa ctgaaaaaga tatttccata ttttcttctg atctttactg aagctgaaaa
     5761 gagatgccca gaattttact gcctttaaat actttttcat aactggatat tgctttttga
     5821 gtaaagtttt actgcctctt gcaagatact ccgcccatgg aaccaaaata cagcagtctg
     5881 accagcatgc tgtcggaatg acaggccaga tatctccatg ccttggtacg atgaacggta
     5941 ttcctccaag ttttccttgt tcatatcgaa catctgcaag ccatttacct agaaatctgc
     6001 ttaaatcaaa attgtaacaa gcagtacgcg aaaagactga aatatctcca gtccatccct
     6061 gccgctcatc tctttgcgga cagtctgtag gtatatcaac aaaattagat ttcgagctcc
     6121 atacaatatt actctgcagt ttgttgatta atggattaga acattcgaaa gctcctattg
     6181 cttcaatatc agaataaatt gcaaatgctg aaacatttat atcttttctt tcaatacctc
     6241 tgattcctac atatcgaaat cccatataag taagctttgg tgagtatatc tgttctcctt
     6301 gtgaacaaat gtatgtaact gtcgcttttg cagttcgtaa agacttaact gacaaattac
     6361 ccttgcatag gatttctgca tgtcttatta cgattttctg attctctctt ccatttatct
     6421 tcagatgtat aactccagca aaattctgac caaagtcata aactacttcc ccattaatag
     6481 caacaaaaac attgactgga agaagagttt catgagcttt tacaggacag ccatatcttg
     6541 cagttatctt aggtttaaac tttaactttg cagtgtcagc tgattcccat gaaactgaat
     6601 ccagttttat tgtcgaatca aaagtttctc catcataaaa atcaccaaac ctataattac
     6661 cattcagtgt taccttccat gtttcatctg ttactatttt ttcaaaactt ccatctgcat
     6721 attcaaaaaa catttctgct aaaagcatct gtcgcttagt ggtaatctgg cttttactgc
     6781 tgtaagtgaa tctacccaca gcccagcctc ctccaactac cgctattaat ttattttcaa
     6841 gatttatata atcagtaata tcataaaata catattgcaa tgtatgtttg taacttgtaa
     6901 atccaggagc aaaatactct tgtgttaaaa cattcccatt taaatttatt tcaaatatac
     6961 ccattgccgt tacagtcatg tatgctcttt ttatttgctt agaagattta aattttttct
     7021 caaatgtcat tgggatcggg gattttttct tttcgggtgt gtatgaggtt ttagtaatcc
     7081 atttggcatc ccatatagtt ttcattcgtc cagtctgaaa atacgcagtt ttcactgcta
     7141 tttcaccatg attatcatat gctgttatat caacaatata catagtaaaa ggtataagca
     7201 cacctgtata aattatatct ttctgagaat atacagtttg ctgccaatcg cctactttaa
     7261 caaccgcctt aactaaatat gtatcttgtt tttcacttaa aagagagaat gagaaaacag
     7321 gattacaatt atcagttata cagccttcta tgagattttc aattctgatt tcatcaatct
     7381 tcaacattaa attccttctc tatgcttaat tctactcaat ttcaaaattc aaaaactgag
     7441 ctttcttctt tcccctgtat gtaaagtaca gaggcttgat tccatcttct atagatatgt
     7501 cttgtttgtg aatctgccaa tcatcagagt aatttaactg tattacagaa atgactttac
     7561 cgggttttat cgaaatctcg aaatacccat ttgcttttcg tactgtaaca gaaattcgtt
     7621 tcacctgctg aaaatcaaaa tacttatacc caataactgt cccatttgaa attgcagtta
     7681 tatactgtcc ttctatacca tgtgtaatat tcggctggcc ctgctcatga ttattaccct
     7741 ggactttttt aagtttctta ttgtaaagat tgcaacaaac agcagctgga tactttcctt
     7801 tattcagaga attacctgaa ccttgagtgg taacctcaac ctggtcaaaa gtcccatcag
     7861 aattcatcat aatccgttcc atgcaagctt gacgggagaa atctgtgtta tgtgtgcaac
     7921 ggtgataaaa gatataataa tcactattaa tgtattcaat agatccgtga atagtcccag
     7981 caggagcaag tctattctta ttaccgagat atcctaagtc actgttggaa atcagaacac
     8041 ctttgtattc aaatcccttg ttaggatatt tactcattgc atatgccagt tcgttatttt
     8101 ctccggatga atatataagg taataggtat tattaaattt acggattgat ggagcttcat
     8161 aaaatgcatg attgtatagc tcactttttt tatcagcagt tgttttacta tctagtacag
     8221 atatcggttc tgatttaaca gtaagcatat cattttcaag ctcagcaaaa gcacaggata
     8281 gaatattggg ttttgttgat ttaatttctt taacagatcg tgaatacagt ttgctttgta
     8341 cagtgttata cagtggagtt agcagtttgc ttctaaaatc cctcccaagt ccccagccat
     8401 agtaaaggta tatcctgcca tcatcattta tgacagcagg atcatttgta agatatttaa
     8461 gaacaggatt accatcagga tattgtatat ctcccaaata ctcaaatggg ccttctggat
     8521 tatctgaaac agcaactgct atagggccga aatttcgcgt atttggtccc atcgctacat
     8581 aatacagata atatcttcca tcgttaccct taacgcagtc aggagcataa tagtcggcaa
     8641 aaccactttc tgtacttctt gggtcttgag atttcttgta actgataccg tgataatccc
     8701 aatgctcaag attatttatc tctgcagaat acacaacata gtcaagctca caaaatctct
     8761 taccgccttg tttgtcatgt gacccgtaga gataaactct gtcaccaaaa acatgaggct
     8821 caccgtctgg aatatattca tttaatggaa gaaatggatt acacatcctc ttttcctatt
     8881 tctcctttac tctttcatgt tgtttattgc ttcgttcatt tctcttctaa gattttcatc
     8941 aattggatag aattttaata taaataagga tattatgcat aaaatcgccg ggacaaggcc
     9001 actaatgata atgaaggaat taagcatctg aggaattgtg gataaatctc caaggaacgt
     9061 atcagtcact gaatccactt gataaccaat tgctataagt acaccaccaa ccagtgcagc
     9121 tgaaagtgcc gtttgtgctt ttcctataaa ccgaccaatt gaattaataa taccatgcat
     9181 ttccttacct gttttataca taccataatc catgctctct atgccgatta atgtgcccgg
     9241 gacaaaactc aaaccacctc caaacagtat aaccagaacc ataataaaaa atattgcagg
     9301 agtttttacg agaagacctg ataacataaa tataaacaaa ccaaggcctc ctgccaattc
     9361 cagccacatg gataatttat aaaccttgac tggccctttg aacaatttta ctaatttagg
     9421 tgatattgca gctgcaataa tcgatggtaa caattgaaaa attccaagaa tcattgtaag
     9481 ggtgccaaat cgtgcctggt caacaacacc tgtcgttaaa tctgtgcaat atgcccactt
     9541 tatgtaatat gtggtagtag caaaaacaag agtccataca aaaccatgaa aaatactaac
     9601 aagcatgctt atgagcagag gtttgttggt tttgagcatt ttaccaatat ctttaaatga
     9661 tattttttgg ctttcttctt cctccatatg ttttccttct ttaacaaaag aaataccgat
     9721 aagagagata atgcccgcaa ataccataaa agcaattgca agaatagaaa aactcctgct
     9781 catatctcca acacttttgt ttattgtagt cataatagat atgaagaatg acataggtat
     9841 aacaataatc attgatatta ctctaggcca agtagtcata cgtgaacgta aaacaggatc
     9901 agttgtcagt gattgcctta gaggattttc tgcaaagaag gatactccta catcatacat
     9961 taaatagaaa aacgcaaccc atatagtaag taaaaccgga ttttgcgcta tagaaaccgg
    10021 cagaccgaat atgaaacata atgccaaggt tgttaaaatt gtgctaagga tgataaatgg
    10081 tttatacttt ccaagttttc ctggtttagc tctatccata atataacctt gtaatggatc
    10141 atccactgca tcaacaattc gaccgaatat caacagcatt gtacccagtg ttgcagcata
    10201 cgctccgata ttagcataat cggttaaaaa caacataaac agacctgtca taaaggctcc
    10261 cgaaactgtt tctgtccagt tcattgtaga caagccaaac atggttttaa aatccgccgg
    10321 ataagatttc ttagttctta aattattacc cataattttt ctcctctttc atttaatatt
    10381 aaaggcattc agcctaaaat ccttcaataa ctgcactttc aaatatcata ttcaaaacat
    10441 tagctgcaca tcgtaatata tcatcacgat ttattgagtc atttttaaga cctttaagta
    10501 aagctttttt tgctgcttta cttcctggca ttataagatc tgttccacag acagggcata
    10561 attcatgctt gcccttatca ctttcagtag agttccagtc agtcatagtc atcccgtcat
    10621 atccccattc acaccttaat acctttgtta ataagtcaaa actattcgca gtatatacac
    10681 cattaaccaa gttatatgag ctcataactg ctttcgggtt ggaatgttcc actgcatacc
    10741 tgaaaccttt aagataaatt tctctaagtg cacgttcttt tatatttgca gaaacatact
    10801 cacgattatc ttcctgatta ttacagcaaa aatgcttaac agtaacacca atacccttgt
    10861 ggctttgtac gcctttactt actgcagaag acatcattcc agtaagtata ggatcttcag
    10921 agtaatattc aaagtttcta ccacatagag ggttacgatg gatattcatt cctggagcaa
    10981 gccagagcgt aacacctgtt tctatcatct ctactccaac tgcattacca accttttcaa
    11041 taagaggaag attccatgtc tgtgcttgaa gcgttacagc aggccaagca gtcataaact
    11101 gataaacagg ggtacctctg cctgcttttg cttttacaaa aggttctatt tttttaagcc
    11161 aaccccattt ccacatagct ggtataccat ctatatatct catacttcca cttttcaaaa
    11221 tcacaatttt tggtgtcagg tttaagccag ctgggccatc gctgaaatta acattaggta
    11281 tactcttttt taacagtgaa actgtcgtac gtccagcaca accaggtgta ttattaaatg
    11341 acttcccaga atatcctgct ccgacaataa gttctataag ttccttgtca tttagattca
    11401 atagaagctg ctctgttttt agatttgttt ttatcttagg attttcatac acatgcttta
    11461 cataagatat atcatcagat ctgacttcaa accttggcaa attcaaatcg tattcatgaa
    11521 taggaaattc tgctttcaat tcactaaaat catattccat cgggcatatg cttgtacatt
    11581 tttctacaac tgtttcattt tccagtacta ctactgcgac tggttcattc tgtcgtgatg
    11641 aagttcccag acatatacca tattcacctt ggtctagtac ccagtttgaa tctttttcat
    11701 catatgaagc atggtcacat aaatcaaatg taagtgtaag taattcattt tcatctggtt
    11761 tcaaattttt cgttttaaca aacgcaacca gacttcttaa ttcatgacag agccttccat
    11821 ttggtttttc cagatataac tgtaaaacct gtttgccaga aaattcagtg cctgtattag
    11881 ttatcatgac cttaatagtt acttttgttt tctgtatatg aatatcactc acatggtgcg
    11941 aaaatgttgt ataagataat ccgaatccaa actcaaaaag aggattaatt ccaaagctgt
    12001 caaaatagcg ataccccaca taaattcctt ctttatagtc ttcgtcctta aggttaccat
    12061 taagatatcc ataatccata gctgaaggat aatcttcata acgttttgcc caggtatcag
    12121 ttattttgcc acttgggttg atcttcccgc ttacaatatc agcaaaagca tttccgccct
    12181 cttcaccacc ttgcacataa tacagtactg caccgatgtt atttgtttca tttaagatgg
    12241 ataaatccaa aatacctcca cagttaataa caagaagtaa tttttcatat tgttctgcta
    12301 atgaatgtat attgtatgat tcctcttcag acagtttgaa gtctccattc tcaaaacgtc
    12361 tatcagcact ttccccagcc tggcgtgcaa ctacatatat tgcagtatca gtattagcac
    12421 ttaagtcttc aatctggatt ttatcaccac agggaaaaca aagaggattt acatgaatga
    12481 cttcatcaaa catcttctga acctgccatg gcttatatgt tttgatcttt tcttcaatac
    12541 tttgtctcca aattttatct ttttgtgcat aatactgatc aaatctatca agccatcgtg
    12601 ttgaaacaat ttcaaaccca gcatttctta acccctgctc aatactaaca ctgtatctct
    12661 cttgcatatc accgctgcct gtcccacctt tgacagtcat acgggctcct gcaccgaata
    12721 aagcaatttt tttatttctt aagggaagta cgccttcatt cttaagcaaa actattcctt
    12781 cacctgcaac ttcgcgacaa agttgacgat gctgtttctc ccgttgtgat tcatcatcag
    12841 tccttaaacc gaaaagtttg aattttgcca ttttactgca cctcgatttc tagtgttaca
    12901 gactgcacac ctgatgattt tacttctatt attgtcttac cagttttata tccagctctt
    12961 agtactgcta ttgtacgacc acggtacgaa ttatggtatt gcttatcaaa aacttcatct
    13021 gtttttgcta gagcagaacc gaaacctgcc agaacagccg gacccgttat ttctaactca
    13081 accttttctt ctataaatgg ttttatattc ccacctttat ctgtaaattc tatttcgata
    13141 aagcacaaat caatcccatt tgcataaatg gatcttttgt caggtcttac acttaatatg
    13201 gtttcaatgc tgcctgatac aagactgtga actgagataa cttttttatt actatctagt
    13261 gcttcagcca taagtattcc gttttgatac tttgttttaa acagagttct aaacttcttt
    13321 actttcttag tacctatctc ttttccatta agtgataatc taacaaattc tgaattgcta
    13381 taaatttcaa caatagcctg aactccttca taaccttgcc aattccagct gtctatacaa
    13441 tccgtgaatc tccatgcact cttgcttgga atttcgtgag cgtgattaag tggatgtatt
    13501 cctataaagg gtttagttct tagtccccag actacctgtt gaaaatatgc ttcagctccg
    13561 attttgccag ttatatctat agctccagaa cccgcaagca aaggtagtcc tttataactg
    13621 tgatatgtcc agtcacctat acaagcttct ccaagataat cccaggcagc ccatgcgaag
    13681 tcacctataa ttgctttatg cttttttaca cgttcccagt tatatggcaa ctctttgatt
    13741 atagtttcag cacccacaat cattctttca ggatatttga ctacatcata gtcatatcgg
    13801 gatgctgcat agttaagacc aaggatatcc aatgatttcg ctgcaccttt tgatgcttta
    13861 tcaccttttc ttcctttact gactaaaaac attaatggtc ccagtttttg aatcattgaa
    13921 ttaaagaaag ctgatccagt ttttttctcc tttatttttt tattaactgg ttcaagaggt
    13981 ttcgccttgt aatcaccttt atctttatat accccgaatc cagctcgtgc ataaacattt
    14041 aaaagcacat ttattccagc tgttacaggt cgggtagagt ctaaagaacg aacataatct
    14101 gcaagctgct tacaagtttc tacacctttt tttgttgccg tttctgaaac ttcatttcca
    14161 atggaataca ttattacact cgggtgattc ttacagttct ccaccattgt aaaaatatct
    14221 tttttccatt ccgtttgaaa atatcttgca tagtcatggt aatttttagg tatgtaccac
    14281 atatcaaatg cttcatccat tacatacatg cctagcttgt cacaagcatt caataactca
    14341 aaggaagcag gattatgagc acaccttatg gcattgtatc cagcttcttt caaaatacga
    14401 actttccgct cctctgaatc aggatatatg caagctccaa gaaccccatt atcctgatgt
    14461 atacacccgc cacgaagaag aacttcctca ttattaatca acaaaccagt attcgcactc
    14521 cattccagtt tacgtatgcc aaacagaatc ttacgttcat ctgtatttgt tcttatcaaa
    14581 caggtgtata gaaatgggtg ctcagcactc caaagctttg gatttggtat cgtgaattca
    14641 cctatgtttc ctgatattat catcttttcg ttatcatata tatctacggc tatttcacaa
    14701 tttaatgttg tttcagctat aatcctgaca acagcaggat ttatacttaa agtttctata
    14761 tttactcttt tgatatggca tttatccctg atttccaact gcacactccg ataaatacca
    14821 ctacctgagt accatctgca gttaggttct aaagaattgt ctacatttac aataagtgtg
    14881 ttttcacctg cccttacagc atcagatata tctattgtaa aaggcacaaa accgtatttg
    14941 cgtcctgtga atttcacatt atttacaact acatcactat tttgatacac acctttgaaa
    15001 tgtatttcaa tacatttacc tatgtcagat ttatcaagat taaaatgttt ctcatagata
    15061 tattttcctc caggaaagaa accagactgt ttattattcc tgcaataagg atcccttttt
    15121 tcagtctgca tcgcatcatg aggaagattc acctttttag aagaatctcc ttcttttcga
    15181 aatgaccaat tttcatttaa acagaataac atttcaccac ccatgacaag ctatttaaag
    15241 catattacat cactatgctt atattttatc tatatagact tgaaatatat tgtaatttcg
    15301 gatatattat tataaata
//
LOCUS       DAOSXM010000051        15110 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2111
            NODE_2467_length_15110_cov_8.992826, whole genome shotgun sequence.
ACCESSION   DAOSXM010000051 DAOSXM010000000
VERSION     DAOSXM010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864178
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 15110)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15110)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:24:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,261
            CDSs (total)                      :: 2,229
            Genes (coding)                    :: 2,218
            CDSs (with protein)               :: 2,218
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 3 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15110
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2467_length_15110_cov_8.992826"
                     /isolate="AMR_MDS_2111"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            61..1524
                     /gene="gltX"
                     /locus_tag="PLQ68_07265"
     CDS             61..1524
                     /gene="gltX"
                     /locus_tag="PLQ68_07265"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003356788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HXK72488.1"
                     /translation="MSQKEVRTRYAPSPTGFVHIGNLRSALYEYLVAKANRGSFILRI
                     EDTDQKRYVDGAVNVIYDTLRIVGIKHDEGPDIGGEYGPYIQSERKNTYLPYAKQLID
                     MKKAYYCFCTEERLDILREEAEKKKVQFKYDRHCLNLSSEEIQKKLDNNEPYVIRQLM
                     PDSGFTTYNDAVYGDIKFSNDVLEDQILIKSDGFPTYNFANVIDDHLMKISHVVRGSE
                     YITSTPKYINLYNAFGWEPPVFVHLPLVVNKEGAKLSKRSGDAYFSDLLDAGYLPEAI
                     VNFLVLLGWSSGTEQEFFTLSELENVFDISGISKSPSTYDTDKLRWMNGEYIRNKTLD
                     DFVKIASPWIDKAIKRNLDKNKICSMIQKRVEVLNEIPQMIEFLENPVPVDTQLYINQ
                     KSKCDISEAKRILPIIKQKIIEMDVFDHENIYEMFRNTATELQVKNGLVMWPARIAIS
                     GLLVTPGGAVEIAEIIGKDETIKRLDKAIKELEAYNA"
     gene            1517..2485
                     /locus_tag="PLQ68_07270"
     CDS             1517..2485
                     /locus_tag="PLQ68_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HXK72489.1"
                     /translation="MLKYDDLPEYCRDFLSYLETIQNRSVQTVNEYYYDLRMFLRFIK
                     ILKFNSKEPFDEIIVVDINVKILEQLTLSDFYAYMTFLSRTRSASAKTRARKVASIRS
                     FYKYLYSKVKLISTNPSLELETPKLKKTLPRYLNLEQSTNLLDNVSSKHEKRDFAMLT
                     ILLNCGIRISELVGINISDYRDDTIKIFGKGNKERTIYINDACKNAIDEYLMTRPNDK
                     AIDKNALFLSDRHKRISRKTVHVIVKQQLHNAGIDTEQYSAHKLRHTAATLMYKHGNV
                     DIRALQRILGHESIATTEIYTHLDDNQLRNATKSNPLANYKKDNKE"
     gene            2608..3657
                     /locus_tag="PLQ68_07275"
     CDS             2608..3657
                     /locus_tag="PLQ68_07275"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HXK72490.1"
                     /translation="MIIKKFLSVFVSAMFTILIVAGLIAVILINVKQNQVNSSVDNTD
                     SNSSNVNENVNPDWLDRYIETKEEPFNLLLFVSDIKGYDTDTIILLNLDTVNSKISAM
                     SIPRDTLVKNQQKAVSIYSTSTNEILKDEKVIDIFDELLGTDIKYTVRMNITVIEKII
                     DEMGGLYFHIPADMTYYDNGVDPVTGIYDPAQELVIEFKKGEKHLSGSEVVKLLRFRQ
                     PAPGTGSNELSEYYSDGSDINRIEMNKQILNAFFDQMFEMSRRNLISKLTNLLSIVYK
                     DVDMYITLSDAQNILDMIMKSIPTDSNDNYQWQGIQWYTLPGSYESSSSMYYYQMDIP
                     ATSTIINNSFYTTLD"
     gene            3663..4400
                     /locus_tag="PLQ68_07280"
     CDS             3663..4400
                     /locus_tag="PLQ68_07280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72491.1"
                     /translation="MDDQKFINAHEKALSLFEDKRSIGVYFEKKLHAILKFYIEPDQS
                     CHELTVNGFIADIFKNDHIYEIQTSSFNKLRKKLDIYLKDYPVTIVFPIAYNKWLYWI
                     DEENGEITKKRKSPKQGSYFDSIKELYKIKMYLKNKNLSICLVLMDIEEYRILNGWSN
                     DKKKGSSRYERIPIRIENIINFSETSDFKVFLPSNIDTPFTSRQYSDLTKINMKNAQI
                     LLNILSYIGLIKKAGKLKNSILYEINV"
     gene            complement(4486..5994)
                     /locus_tag="PLQ68_07285"
     CDS             complement(4486..5994)
                     /locus_tag="PLQ68_07285"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003867253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HXK72492.1"
                     /translation="MMNIPVIPVATIKNANRGIEMVDNKDVKDLLFYERKNIWKEISE
                     DQVTAINQFSENYKSFLNECKTERECVKYIHNLCLSKENSFISLKSAYKNKQQLHPGD
                     KLYYIENNKAVVLITIGKEGLSQGIKLIGSHIDSPRLDLKPLPLFEDSELGFLKTHYY
                     GGVRKYQWVTIPLALHGIIYKSDGTEVEICIGENDSDPVFVIPDLLPHLAAEQNKLTL
                     KDAIPGENLNLLTGSVPNADTEVSEKVKATILQLLNNKYGITEKDFINAELEAVPALK
                     ARDVGIDKSFLGAYGHDDRVCAYTSLMAFFESSNTINDKTCFCYFSDKEEIGSTGNTG
                     AQCDTMTYVTEMIAELLDVSETKEKSHLNIKGVFRDSVMLSADVTAAFDPNHKSVFES
                     NNAAFINRGIAICKYTGSGGKGGASDANAEFLNSITKLFDQSEDIVWQIAELGKIDVG
                     GGGTIAKFAAALGINVLDAGVPLLSMHAPYELASKADIYMTYKAFKAFYENL"
     gene            complement(5945..6373)
                     /gene="nifU"
                     /locus_tag="PLQ68_07290"
     CDS             complement(5945..6373)
                     /gene="nifU"
                     /locus_tag="PLQ68_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004625690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold protein NifU"
                     /protein_id="HXK72493.1"
                     /translation="MYSSKVMDHFSNPRNVGELPDANAIGEVGNAKCGDIMKIYMKIE
                     DNIVKDVSFKTFGCGAAIATSSMATELVKGKTVEEALDITNKAVMEALDGLPPVKVHC
                     SVLAEEAIKAAINNYKKSIGEQVVDDEYTCNSCCHNKKCE"
     gene            complement(6416..7609)
                     /gene="nifS"
                     /locus_tag="PLQ68_07295"
     CDS             complement(6416..7609)
                     /gene="nifS"
                     /locus_tag="PLQ68_07295"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase NifS"
                     /protein_id="HXK72494.1"
                     /translation="MKHIYLDHAATTQLDKRVLEKMLPYMSDYYGNPSSIYDVGRKSK
                     NAIEEARDTISSCFNCKNTEIYFTSCGTESDNWAIKGVCSFNRNKGMHIITTNIEHHA
                     VLDTFHYMKKLGYDISVVPVEKNGIVDPAKIEKAIRKDTILISVMFANNEIGTIQPIK
                     EIGEIAKKHNILFHTDAVQAVGTIPIDIQNLNIDLMSISAHKFYGPKGVGALYIRRGV
                     MIDNLLHGGGQEKTRRAATENIAGIVGMAEALKIAVDNLEENNNHIKKIRDLVKKGIE
                     KKIPYIIYNGDEEKRLVNNINFCFRFIEGEGLILMLDFKGISSSSGSACTSGSLDPSH
                     VLLALGLPHEIAHGSLRLTFGRDNSLDDVDYIVDTIADVVFKLRQMSPLYETFIKTNQ
                     ANDNQ"
     gene            complement(7671..7937)
                     /locus_tag="PLQ68_07300"
     CDS             complement(7671..7937)
                     /locus_tag="PLQ68_07300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72495.1"
                     /translation="MDEKKKVKLKRIFAWIYLVLFIFVILNLMIFDFYKDIFLFAYIA
                     LILVYFIVFKNVTETNKTTNEINSKDIDHQKEREAGLIDNKDDN"
     gene            complement(7916..>8763)
                     /locus_tag="PLQ68_07305"
     CDS             complement(7916..>8763)
                     /locus_tag="PLQ68_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HXK72496.1"
                     /translation="ISRSSVFMLEPLTAQDIYQILQNALNDKERGLADMDVSIDEETL
                     VKLSSSCSGDARIALNALELAVITTKCDNKGKINITQDDLKECMQNNAIRFDKSGEDH
                     YDNISALIKSMRGSSPDGALFYLGKALVSGEDINFLARRIVICAAEDVGLANPNALVV
                     AQSAAQAVRMIGMPESALILAEAAVYVAASSKSNACSEGILNVMNDIKQTDTGEVPMW
                     LRNAPVEQMKNFGYSQGYKYAHNYEGNYVEQQYLPDIIKDKIYYKPTDNGMEGTIMEW
                     MKKRK"
     assembly_gap    8764..8863
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8864..8994)
                     /locus_tag="PLQ68_07310"
     CDS             complement(<8864..8994)
                     /locus_tag="PLQ68_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015925388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HXK72497.1"
                     /translation="MKREPLAYRMSPRTLDEFVGQKHIVGEKKLLRRLIESDNLHSV"
     gene            complement(8995..10230)
                     /gene="glyA"
                     /locus_tag="PLQ68_07315"
     CDS             complement(8995..10230)
                     /gene="glyA"
                     /locus_tag="PLQ68_07315"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HXK72498.1"
                     /translation="MYKLDSIREIDNELADAIQAEINRQKNNIELIASENFVSSAVMD
                     AMGTPLTNKYAEGYPNRRYYGGCEHVDVVESLAIKYACELFGAEHANVQPHSGANANL
                     AVFFAVLRPNDTVLSLDLSCGGHLSHGMKKNISGSYFNIVSYGVNKDTGMLDYEEIRK
                     LAHECKPKLILAGASAYSRSIDFKKFRDIADEVGAYLMADMAHIAGLVAAGLHQNPVP
                     YADFVTTTTHKTLRGPRGGLILCKEQYKKIIDSAIFPGTQGGPLMHIIAAKAVSFKEA
                     LSDEFKIYQKNTVQNAKDLAFFLSEEGIDIVTNGTDNHLMLCDLRSINITGKDAQAML
                     DSVNVTCNKNGIPYDTQKPTITSGIRLGTPAVTTRGMGKTEMKEIAEIIYNTLTNFDD
                     FKDQAIKRVEYLINKYPLY"
     gene            complement(10261..11007)
                     /gene="trmD"
                     /locus_tag="PLQ68_07320"
     CDS             complement(10261..11007)
                     /gene="trmD"
                     /locus_tag="PLQ68_07320"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009431633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HXK72499.1"
                     /translation="MNTFNIFTLFPEMFTALDYGVIGIAKSKGIICINTINIRDFSKN
                     KHKKVDDYPYGGGFGMLMTCQPVFDAIKSLPKIHTIYMSPKGKVLTQKRAMELSNLQD
                     INILCGHYEGIDQRIIDSLIDEEISCGDYVVTGGEIPAMLLVDSISRLIKGVLPQPES
                     YTDESHQDNLLESYQYTRPEEYDGLSVPNILLSGHHKNISEFRLAQSIYLTYQKRPDL
                     LEHRKINKTEYKIFTIYYPQLKDDIIKFVE"
     assembly_gap    11112..11211
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(11355..11585)
                     /locus_tag="PLQ68_07325"
     CDS             complement(11355..11585)
                     /locus_tag="PLQ68_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HXK72500.1"
                     /translation="MKQLLETIIYALVDDKDQVVITEESDKDGNTVFKISASHNDVGR
                     IIGKNGNTINAIRKIVRAYAIKLRTKVTVDVL"
     gene            complement(11599..11835)
                     /gene="rpsP"
                     /locus_tag="PLQ68_07330"
     CDS             complement(11599..11835)
                     /gene="rpsP"
                     /locus_tag="PLQ68_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007489428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HXK72501.1"
                     /translation="MVKIRLKRMGAKKNPYYRVVVADSRFPRDGRFIEEIGMYDPLSN
                     PAKFEIDMEKANAWLAKGAQCTETVRSLIKKHTA"
     gene            complement(11867..13195)
                     /gene="ffh"
                     /locus_tag="PLQ68_07335"
     CDS             complement(11867..13195)
                     /gene="ffh"
                     /locus_tag="PLQ68_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014643248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HXK72502.1"
                     /translation="MAIFEGLTEKLGSIIKGLRNTGRITENDLKNISREIRLALLSAD
                     VNYKIVKDFVASISEKALNKDVLESLTPGQQVIKIVHEELILLLGGKNEKLITSSNIP
                     NKYMLCGLQGSGKTTACGKLGNMLKKQNKKIMFVACDVYRPAAIDQLIILGKQLDIPV
                     YSEKNEKNVIKIAKNALDYSRKNIIDILIFDTAGRLHIDENMMDEIHTLQKTINPDET
                     LLVVDSMTGQDAVNIAEGFSSKLDITGIILTKLDGDTRGGAALSIKKIVDKPIKFASV
                     GEKMNDLEPFHPDRMASRILGMGDVLTLIERAQQNFDEKKAVELEKKMATSSFTLQDY
                     LEQLEQFKDMGSMQEMLNMIPGGASLKDIKVDEKQIIRTKAIIQSMTAKERKNPRLLD
                     AKRRKRIASGSGTSVTDVNRMLKGFEDMKIMMKKMTSSKGKKFKGLNMPF"
     gene            complement(13196..13516)
                     /locus_tag="PLQ68_07340"
     CDS             complement(13196..13516)
                     /locus_tag="PLQ68_07340"
                     /inference="COORDINATES: protein motif:HMM:NF016205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HXK72503.1"
                     /translation="MDNYSRAAILIDIYGSLLTEKQFNALCLYLNEDCSLFEIAEKMG
                     ISRQAAHDIIKRSMNLLEEYELNLGIYSKLIVNRKNENVIKKLLIENKVNEALSLVHD
                     IVEN"
     gene            complement(13661..15037)
                     /locus_tag="PLQ68_07345"
     CDS             complement(13661..15037)
                     /locus_tag="PLQ68_07345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72504.1"
                     /translation="MAKILLNTANIICNNKKETDLRAYVNGKVINTLNYDSKEVIMEY
                     ADTDFVFLIDENMETDDSKTDVSLMREIRRFTKNYKESDVETKFSDFEECISTTVTYL
                     ENLEETGEISPMNRQVSAALISYDRALLATTDTDSAIYISGGNIVKVFPEDDDSKLKT
                     AQLPALKTNDLIILLSNTISNSISAVDILDRINTYDSAEAIAQSIASTYSAINGEEFS
                     VSVILVKLIQDKSRTFLRSEINTQEIPTDDVQKMYTAPIAAAKFEGKPSKKESFWDGT
                     SDDVVFADKKNKSAKGSSFDSIKLSKSESRKRRTSIYIKRAVSIIVVLALMAGIIWGM
                     VKLLGAIFNKEASNESPTPSVVVSPIVVDSPSPTQTPVVSESAIPTDSETAEPTPETQ
                     YIIHKVKITTDHVSLNQIAAYYNDVYSLNYPNTAIFVEVIAGLNEISDVNMIYVGQEI
                     KIPITPVP"
BASE COUNT         4876 a         2471 c         2138 g         5425 t
ORIGIN      
        1 aagaagcatt taacagaatg ccatgattct gtctaatatt aatatacaag gagaagatat
       61 atgtctcaaa aagaagtaag aacacgttat gcgccatcac caacaggatt cgtacatata
      121 ggcaatctaa gatctgcact atatgaatat cttgtagcaa aagctaatag aggaagtttt
      181 atattgagaa tagaagatac tgaccagaaa agatatgtag atggagctgt caatgttata
      241 tatgacactt tgagaattgt aggtatcaaa catgatgaag gtccagatat tggaggagaa
      301 tacggcccat atatacagtc agagagaaaa aatacatatc ttccatatgc aaaacaactt
      361 attgatatga aaaaagctta ttattgtttt tgtacagaag aaagattgga cattttaaga
      421 gaagaagctg aaaagaaaaa agttcaattt aaatatgaca gacactgttt aaatctatct
      481 tctgaagaaa ttcaaaaaaa attggataat aatgaaccat atgttataag acagttaatg
      541 ccagattctg gttttactac atataatgat gcagtatatg gagatatcaa atttagtaat
      601 gatgttttag aagatcagat actgattaaa tcagatggtt ttccaacata taattttgct
      661 aatgtcatag atgatcatct tatgaagatt tcccatgtcg taagaggcag tgaatatatt
      721 acatctaccc ctaaatacat taatctttac aatgcatttg gatgggaacc tcctgttttt
      781 gtgcatttac ctcttgttgt aaataaggaa ggagcaaaac tttcaaaaag aagcggtgat
      841 gcatatttct ctgatcttct agatgccggt tatcttcctg aagcaatagt taattttctt
      901 gtattgctag gatggagcag cgggactgag caggaatttt tcacactcag cgaactcgaa
      961 aatgtatttg atatttcagg cataagtaaa tctccatcca cctatgatac agataaacta
     1021 agatggatga atggtgaata tataagaaat aagactttag atgattttgt taaaatagca
     1081 tcaccctgga tagataaagc tattaaaaga aatcttgata aaaacaaaat atgttcaatg
     1141 attcaaaaac gtgtagaggt tctaaacgaa atacctcaga tgattgaatt tttagaaaac
     1201 cctgttccag tagatacaca gttatatata aatcaaaaaa gtaaatgtga catttcagaa
     1261 gcaaaaagaa tattgccaat aataaagcag aaaataattg aaatggatgt ttttgatcat
     1321 gagaatatat atgaaatgtt tagaaataca gcaactgaat tgcaggttaa gaatggatta
     1381 gtaatgtggc cagctcgtat agcaattagc ggactattag ttactccagg aggtgcagtt
     1441 gaaatagctg aaataattgg taaggacgaa acaattaaaa gattggataa agctataaaa
     1501 gaactggagg catataatgc ttaagtatga tgatcttcct gaatattgta gggatttttt
     1561 atcatacctt gagactattc aaaacaggtc tgttcagaca gtaaatgaat actattatga
     1621 tttacgtatg tttctacgtt ttattaaaat attgaaattt aactcaaaag aaccttttga
     1681 tgaaataatt gttgttgata ttaatgtgaa aatacttgaa caacttacac tatctgattt
     1741 ttatgcctat atgacttttc tatcaaggac tagatcagca tcagcaaaaa ctagagcaag
     1801 aaaagtagca tctatcagat ccttttataa atatctatac tcaaaagtga aacttatcag
     1861 cactaatcca tcactcgaat tagagacacc taaattaaag aaaacacttc cccgctatct
     1921 caatcttgaa caaagtacaa atttacttga taatgttagc agcaaacatg aaaaaagaga
     1981 ttttgcaatg ttgactatat tattaaactg tggaataaga atttctgaac ttgtaggaat
     2041 taatataagt gattaccgtg atgacacaat aaaaattttc ggaaaaggta acaaagagcg
     2101 tactatttac attaatgatg cttgtaaaaa tgctattgat gaatacctta tgacaagacc
     2161 taatgataag gctattgata agaatgcgtt atttttaagc gacagacata aaagaataag
     2221 cagaaaaact gtacatgtaa tagtcaaaca gcaattacat aatgcaggga ttgataccga
     2281 acaatactct gcacataaat taagacatac tgcagcaact ttgatgtata aacatggtaa
     2341 tgttgatata agggcattac aacgaatttt aggtcatgaa agtattgcaa caacagagat
     2401 atacacacac ctagatgata atcaacttag aaacgctact aaaagtaatc ctttagctaa
     2461 ttacaaaaag gataacaaag aatgattttg gaacttatac ttgttataac acgttaaatt
     2521 actagcaatt gcaaaagatt tgcaacgctt tacattaata atcgtatatt gtaaaatgta
     2581 tgaagttaat ggttacaagg aggcagaatg ataataaaaa aattcctttc agtatttgta
     2641 tcagctatgt tcacgattct tatagtcgct ggtttgattg ctgttatttt aataaatgtt
     2701 aagcaaaatc aagtaaattc tagtgttgat aataccgatt caaattcttc taatgttaat
     2761 gagaatgtta atcccgattg gttagataga tatattgaaa caaaagaaga accgtttaac
     2821 ttattgcttt ttgttagtga tattaaagga tatgatactg atacaattat tttattaaat
     2881 cttgatactg ttaatagcaa aattagtgca atgagtatac caagagatac attagtaaaa
     2941 aaccagcaaa aagcagtatc aatttattca acttcaacca atgagatact caaagatgaa
     3001 aaagtaattg atatctttga tgaactttta gggactgata ttaagtatac agtaagaatg
     3061 aatattactg taattgagaa gataatcgat gaaatgggtg gattgtattt ccatatacct
     3121 gctgatatga catattatga taatggtgtt gatcctgtta caggaatata tgatcctgcg
     3181 caggagcttg ttatagaatt taaaaaaggt gaaaaacatc tttcagggtc tgaagtagta
     3241 aaacttttaa gattcagaca gcctgcacct ggaacaggat ctaatgagtt atcagaatat
     3301 tattctgatg ggtcggatat aaacaggata gagatgaata aacaaatttt aaatgccttt
     3361 tttgatcaga tgtttgaaat gagcagaaga aatttaatta gtaagttaac aaacttgtta
     3421 tcaattgtat ataaggatgt tgatatgtat attactttat ctgatgctca gaatatacta
     3481 gatatgataa tgaaatcaat acctacagat agtaacgata attaccaatg gcaaggaata
     3541 cagtggtata cacttcccgg gtcatatgaa agttcatcta gtatgtatta ttatcagatg
     3601 gatattccag caacttcaac aataataaat aattcatttt atactacatt ggattaaggt
     3661 aaatggatga tcaaaaattt ataaatgcac atgaaaaggc gttgtctcta tttgaagata
     3721 agcgttctat aggtgtgtat ttcgaaaaaa aacttcatgc aatattgaag ttttatatag
     3781 aaccagacca gtcttgtcat gagttaactg taaatggttt cattgctgat attttcaaaa
     3841 atgaccatat atatgaaatt caaacttcat ctttcaataa actgagaaag aaacttgata
     3901 tttatctaaa agattatcct gtaacgatag tattccctat tgcgtataat aaatggctat
     3961 attggataga tgaagaaaat ggtgaaatta caaaaaaaag aaaaagccct aaacaaggtt
     4021 catattttga cagtattaag gaattatata aaattaaaat gtatttgaaa aataagaatt
     4081 tatctatttg tcttgtatta atggatattg aagaatacag aatacttaat ggttggagta
     4141 acgacaaaaa gaaaggttct tcacgatatg aaagaatacc tattagaatt gagaatatta
     4201 ttaatttttc tgaaacatcg gatttcaaag tatttttacc atccaatatt gacactccct
     4261 ttacatcaag gcaatactca gatttaacta aaataaatat gaagaatgct caaattttat
     4321 tgaatatact ttcttacata ggtttaatta aaaaagcagg aaagcttaaa aattccattt
     4381 tgtatgagat aaatgtttaa taaaatcaaa ataaaataaa acggaactta ttatgttccg
     4441 tttttaagtt tatttttttt aaataaagct actataaatg attaattata aattttcata
     4501 aaaagcttta aatgctttat atgtcatgta tatatctgct ttactggcta attcgtacgg
     4561 agcatgcatt gacaatagag gtacacctgc atcaagaaca tttataccta atgcagctgc
     4621 aaattttgct attgttccac caccaccgac atctattttt cctaattctg caatctgcca
     4681 gactatatct tctgactggt cgaaaagttt ggttattgag tttaagaatt ctgcattagc
     4741 gtcacttgct ccacctttac caccagaacc tgtatatttg catattgcaa ttcccctgtt
     4801 aataaaggca gcattatttg attcaaatac tgatttatgg ttagggtcaa atgctgcagt
     4861 aacatctgct gaaagcatta cagaatctct gaacactcct tttatgttca aatggctttt
     4921 ctctttggtt tctgaaacat caagtaattc tgcaatcatt tcagtaacat atgtcatagt
     4981 atcacattga gcaccagtat ttcctgttga tcctatttct tctttgtcag aaaagtagca
     5041 gaaacatgtt ttatcattta tggtattact tgattcaaaa aatgccataa gtgaagtata
     5101 tgcgcatact ctgtcatcat gtccatatgc acccaaaaaa gacttgtcaa taccgacatc
     5161 tcttgctttt aatgctggaa cagcctctaa ttcagcattt ataaagtctt tctctgtaat
     5221 accatactta ttattaagta actgcaaaat cgttgcttta accttttcag atacctctgt
     5281 atcagcatta ggaacagaac cagttaaaag attgagattt tcacctggaa tagcatcttt
     5341 aagtgttaat ttattctgct ctgctgcaag atgaggtaaa agatctggaa ttacgaaaac
     5401 aggatctgaa tcattctcac caatacagat ttcaacttct gtaccatcac ttttatatat
     5461 tattccatga agtgctaatg gtatagttac ccattgatat tttctaactc ctccataata
     5521 gtgagttttt aaaaatccaa gttcagagtc ttcgaataat ggtagtggtt ttaaatctag
     5581 ccttggagaa tcaatgtgtg atcctattaa cttaataccc tgagatagac cttctttacc
     5641 tattgttatt aaaacaactg ctttattatt ttcaatatag taaagtttat ctccaggatg
     5701 aagctgctgt ttgtttttgt atgcgctttt tagagatata aaactatttt ctttgcttaa
     5761 gcataagttg tgtatatatt taacacattc tctttctgtc ttacattcat ttaagaaact
     5821 tttataattt tctgaaaatt gatttattgc tgttacttga tcttcagata tttctttcca
     5881 aatgtttttt ctttcataaa agagtaagtc tttaacatct ttgttatcta ccatttctat
     5941 acccctattc gcatttttta ttgtggcaac aggaattaca ggtatattca tcatcaacta
     6001 cctgctctcc tatgcttttt ttataattat ttatagctgc tttaatagct tcttcagcta
     6061 gaactgaaca atgtactttt acaggaggta aaccatctaa ggcttccata acagctttat
     6121 tcgtaatatc taaagcttcc tctactgttt ttccctttac aagctctgtg gccatactgc
     6181 ttgtagctat tgcagctcca cagccgaatg ttttaaagct aacatcctta actatattat
     6241 cttcaatctt catatatatc ttcattatat ctccgcattt agcattacct acttcgccaa
     6301 tagcattagc atcaggtaat tcgccaacat tacggggatt tgagaaatga tccattactt
     6361 ttgaactata catctgcgtt ctcctttcat tcattgtatt ataacatatt tatacttact
     6421 gattatcatt tgcttgattt gttttaataa aagtttcata taatggagac atttgtctaa
     6481 gtttaaatac tacatctgct atagtatcaa caatgtaatc aacatcatct agtgaattat
     6541 cccgtccaaa tgttaatctt aatgaaccat gagcaatttc atgaggcaga cctaaagcta
     6601 aaagaacatg tgaaggatct aaggatcctg aagtgcatgc tgaaccggat gaagaactta
     6661 ttcccttgaa atctaacatt agtattaatc cttcgccttc tataaatcta aaacagaaat
     6721 ttatattgtt aacaagtctt ttttcttcat caccattata aataatgtaa ggtatttttt
     6781 tctcaatacc ttttttgacc agatctctta ttttcttaat atggttatta ttttcttcca
     6841 aattatctac agcaattttt aaagcttcag ccataccaac tataccggct atattctcag
     6901 ttgcagctct tctagtcttt tcctgtcctc caccatgaag aagattatca atcatgactc
     6961 cccttcttat atataaagct ccaactcctt ttggaccata aaacttatgt gctgatattg
     7021 acatcaaatc aatatttaaa ttttgaatgt caataggtat agtgccaaca gcttgtacag
     7081 catcagtatg gaataatata ttatgttttt ttgcgatttc tccaatctct ttaattggct
     7141 gaatagttcc tatctcatta ttagcaaaca taacagaaat taaaatagta tcttttctta
     7201 tggctttttc aatttttgca ggatctacaa ttccattttt ttcaacaggt actacggaaa
     7261 tgtcatatcc aagttttttc atataatgaa aagtatctaa tactgcatga tgttctatgt
     7321 tagttgttat tatgtgcatt cccttatttc tattaaatga acaaactcct ttaatagccc
     7381 aattatcact ttctgtgccg catgaagtaa aataaatctc tgtgttttta cagttaaaac
     7441 aagaggatat tgtatctctt gcttcttcaa tcgcattttt actttttctt ccaacatcgt
     7501 aaattgatga tggattacca taataatcac tcatgtatgg taacattttt tccaaaactc
     7561 ttttatcaag ctgagttgtt gctgcatggt ccaaatatat atgtttcata aattctccac
     7621 tttctaataa ttcaataaaa taatatgtta attaattaac tttatatatt ttaattatca
     7681 tctttattat cgattaatcc agcttctctt tctttttgat ggtcaatatc tttactattt
     7741 atttcattag tagttttatt tgtttctgta acatttttaa atactataaa gtaaacgagt
     7801 attaatgcta tataagcaaa aagaaatata tccttgtaaa aatcaaaaat cataagattc
     7861 agtattacaa aaataaataa gaccagataa atccaggcaa aaatcctttt cagttttact
     7921 ttcttttttt catccattcc attattgttc cttccatccc attatcagtt ggtttataat
     7981 atatcttatc ttttattata tcaggcagat actgttgttc aacataattt ccttcataat
     8041 tgtgagcata tttgtaacct tgtgaatatc caaaattttt catttgttct acaggtgcat
     8101 ttctcaacca cataggcact tcgcctgtat ctgtttgttt tatatcattc ataacattta
     8161 aaataccttc tgaacatgca ttggacttag aagatgcagc tacataaact gcggcttcag
     8221 ccagaataag tgctgattca ggcatgccta tcattcttac agcttgtgct gctgattgtg
     8281 ctactactaa agcattagga tttgcaaggc ctacgtcttc tgctgcacat atgactattc
     8341 ttctagcaag aaagttaata tcttctccgc tgactaatgc ttttccaaga taaaataatg
     8401 caccatctgg tgaagatcct ctcattgatt taattaatgc gcttatatta tcataatgat
     8461 cttctccaga cttatcaaat ctgatcgcat tgttctgcat acattccttg agatcatctt
     8521 gagtgatatt tatctttcct ttattatcac atttagttgt aattacagct aattctagtg
     8581 catttaacgc aattcgcgca tcacctgagc atgaagaaga tagtttcaca agtgtttctt
     8641 catcaataga aacatccata tctgctaatc ctctttcttt atcgttaaga gcattctgga
     8701 gaatctgata tatatcttga gcagttaatg gttcaagcat aaaaacagaa gatctggata
     8761 ttannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnataactg aatggagatt
     8881 atcagattct ataagtcttc ttaataattt tttttctcca acaatatgct tttgtcctac
     8941 aaattcgtcc agtgtacgag gactcattct gtaagctaaa ggttctcttt tcatttagta
     9001 taaagggtat ttatttatta aatattcaac tcgttttatt gcttgatcct taaaatcatc
     9061 aaaatttgta agtgtgttat aaattatttc agcaatctct ttcatttcag ttttacccat
     9121 tcctctagta gttactgctg gagtaccaag tctaatacca cttgttattg taggtttttg
     9181 agtatcataa ggtataccat tcttattgca tgttacattc acagaatcaa gcattgcttg
     9241 tgcatcttta ccggttatat tgatacttct taaatcacac agcataaggt gattatctgt
     9301 accatttgta actatatcaa tcccttcttc agaaagaaaa aaagcaaggt cttttgcgtt
     9361 ttgaacagta tttttttgat agattttaaa ttcatctgat aatgcttctt taaaactgac
     9421 tgctttagcc gcaataatgt gcatcaatgg accaccctga gtacctggga aaatagcact
     9481 atcaattatt tttttgtatt gttctttaca caggataaga ccacctctag gacctcttag
     9541 agttttatgt gttgtagttg taacaaaatc tgcatatggt actggattct ggtgtaaacc
     9601 tgctgcaact aatcctgcaa tatgtgccat atctgccatc agatatgctc ctacttcatc
     9661 tgctatatct ctaaattttt taaaatcaat agatcttgaa taagcagacg ctccagctaa
     9721 aataagtttt ggcttgcact cgtgtgcaag ttttcttatt tcttcataat ctagcattcc
     9781 tgtatcttta ttaactccat atgaaactat attgaaataa cttcctgaaa tatttttttt
     9841 cataccatga gagagatgac ctccacatga aagatcaagg cttagtaccg tatcatttgg
     9901 tcttaaaact gcaaagaata ctgctagatt agcatttgca cctgaatgag gctgaacatt
     9961 tgcatgttct gctccaaata gttcacaagc gtattttata gctagacttt ctacaacatc
    10021 aacatgctca caaccaccat aataccttct gtttgggtac ccttctgcat atttgtttgt
    10081 taaaggagtt cccatagcat ccattacagc actgctgaca aaattctcag aagctattaa
    10141 ttcaatatta tttttctgtc tgttgatttc tgcttgaatc gcatcagcca attcattatc
    10201 aatttcacgg attgaatcta atttgtacat aaatattctc catttcaatt attcattcaa
    10261 ttattcaaca aattttatta tatcatcttt tagttgggga taatatattg taaatatttt
    10321 atattctgtt ttatttattt ttctatgttc taacaagtcg ggtctttttt gataggtaag
    10381 gtatatgctt tgtgctagtc taaattcaga aatattttta tgatgaccag ataataagat
    10441 attgggtact gataatccat catattcttc aggtcttgta tattgataac tttctagtaa
    10501 attatcttga tgagattcat ctgtgtaact ctcaggttga ggtaataccc ctttaataag
    10561 acgacttatc gagtcaacaa gtaacatcgc tggaatctct cctcctgtta cgacataatc
    10621 accacatgaa atctcttcat ctatcaaaga atcaataatt ctctggtcta ttccttcata
    10681 atgtccgcat aatatattta tgtcctgtaa attcgataat tccatagctc ttttttgtgt
    10741 taaaactttt cctttagggg acatatatat agtatggatt tttggtaaac ttttaattgc
    10801 atcaaataca ggttgacatg tcattaacat accaaaacct cctccgtatg gataatcatc
    10861 aaccttttta tgcttatttt tagaaaaatc tcttatatta attgtgttta tacagattat
    10921 ccccttactt tttgcaattc caataacacc gtaatctaaa gcagtgaaca tctctggaaa
    10981 caaagtaaat atattaaatg tattcatcat ctagtaatcc ttttggtatc ttaacatgaa
    11041 ttctcttagt attaatgtca atatttaaaa caacaccttt tattgcaggt ataagtatct
    11101 gttgtttgta tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nattacgcta
    11221 aattctatac cttgaattat aacttttttt atttttagaa aattcgatgc atcacgggtt
    11281 ataggaaaga cttttagctc tccatttaaa ccgtgtgcac caataatttt tcccaattct
    11341 aataaatcat ccaactatag cacatccaca gtaacttttg ttctcaattt tattgcgtaa
    11401 gctctaacga tttttcttat agcatttatg gtatttccgt ttttcccgat tattctgcca
    11461 acatcattat gtgaagctga tatcttgaaa actgtattac catctttatc tgattcttca
    11521 gttatgacaa cttggtcttt gtcatcaact aaagcataaa tgatggtttc taataattgc
    11581 ttcattatac ttcctccatt aagcagtatg cttttttatt aaagacctaa ctgtttctgt
    11641 gcattgtgcg ccttttgcta accatgcatt agctttttcc atatcaattt caaattttgc
    11701 tgggtttgaa agtggatcat acattccgat ttcttcaata aatcttccat cccttggaaa
    11761 tcttgagtca gctacaacaa ctctgtaata tggattcttc ttagcaccca ttctttttaa
    11821 tctgatctta accatttttt atatcctcca tttattgttt gtttattcaa aaaggcatat
    11881 ttaatccttt gaatttcttt ccttttgaag atgtcatttt tttcatcata attttcatat
    11941 cttcaaatcc tttgagcatt ctgttaacat cggtaactga tgtaccgctt ccagatgcaa
    12001 ttctctttct gcgttttgca tcaagtaatc taggattttt tctttcctta gctgtcattg
    12061 actgaataat agcttttgtc cttattattt gtttttcatc tacctttatg tcttttaaag
    12121 acgcaccacc aggtatcata ttaagcattt cttgcatact tcccatatct ttaaactgtt
    12181 caagttgttc aagataatct tgaagtgtaa aagaagatgt tgccattttc ttttcaagct
    12241 caactgcctt tttttcatca aaattctgtt gagctctttc tattaatgta agaacatcgc
    12301 ccattccaag aattcttgaa gccattctat cgggatgaaa aggttctaaa tcgttcatct
    12361 tttcacctac tgaagcaaat tttattggtt tatcaacaat ttttttaata gataatgctg
    12421 cgccacctct tgtatctcca tccagtttag taagaattat tccagttata tcaagttttg
    12481 aactaaaacc ttctgctata tttactgcat cttgaccagt cattgaatca actactaaaa
    12541 gtgtctcatc aggattaata gttttttgca gggtatgaat ttcatccatc atattttcat
    12601 caatgtgaag tcttcctgcg gtatcaaaaa ttaaaatatc gattatattt tttcttgaat
    12661 aatcaagagc gttcttagca attttaatta cgtttttctc attcttttca ctgtaaacag
    12721 gtatatcaag ctgtttgcct aaaatgatta attgatctat agctgctggt ctatatacat
    12781 cacaagcaac aaacattatt ttcttgttct gttttttcag catatttcct aattttccac
    12841 atgcagttgt tttaccagaa ccttgtaaac cgcataacat atacttattt ggtatatttg
    12901 aagaagtgat aagtttttca ttttttccgc ctaatagcaa gataagttcc tcatgaacaa
    12961 ttttaattac ctgctgacca ggagtaaggc tttccaacac atccttattg agtgcttttt
    13021 cagatattga agctacaaaa tcttttacaa ttttataatt tacatcagca gaaagtaatg
    13081 ctaaacgtat ttctcttgaa atatttttta aatcattttc ggtaattctt cctgtatttc
    13141 taagaccttt aattatcgat cctaattttt ctgttaagcc ttcaaaaatc gccatttaat
    13201 tctccacaat atcatgcaca agacttaaag cctcatttac tttgttttct atcaataatt
    13261 tcttgattac attctcgttt tttctattaa ctattaactt tgaatatata cctaaattta
    13321 gttcatattc ttcaagcagg ttcattgacc tttttattat atcatgtgca gcttgacggc
    13381 ttatacccat tttttcagca atttcaaata atgaacagtc ttcatttaag tataggcata
    13441 aggcattgaa ctgcttctca gttaataatg atccatatat atctattaat atcgctgctc
    13501 tactataatt atccataaac acctgtaaag taatattact tgacacataa tacagcaata
    13561 agattaactt gtcaataaaa aaagaagcat tgcttctttt aatataaatt taatacttta
    13621 ctatttaaat atagaacaat aaattaaaat tgttgagtta ttacggaact ggcgtaatag
    13681 gaatttttat ttcctgtcct acataaatca tatttacgtc agatatttcg ttcaacccag
    13741 caattacttc aacaaatatt gcagtattag gataatttaa actgtataca tcgttatagt
    13801 atgcagctat ttgatttaaa ctgacatggt cagttgtgat tttaacttta tgaattatgt
    13861 attgtgtttc tggtgtaggt tcagcagttt ctgagtcagt tggtatagca gattctgaaa
    13921 caacaggtgt ttgagttgga gatggtgaat caactactat tggagaaaca acaacagaag
    13981 gagttggcga ttcgtttgat gcttctttat taaatattgc accaagaagt ttaaccattc
    14041 cccatattat tcctgccatt aacgctaata ctacaataat gctcactgca cgttttatat
    14101 atatagatgt tcgtcttttt ctgctttcgc ttttactgag ttttatcgaa tcaaatgatg
    14161 atccctttgc agatttattt ttcttatcag caaatactac atcatctgaa gtaccatccc
    14221 agaaactttc ttttttagat ggctttccct caaactttgc agctgcaatg ggtgcggtat
    14281 acattttttg aacatcatca gtaggtattt cctgtgtatt aatttcactt ctcaaaaatg
    14341 ttcttgattt gtcttgtata agtttaacta aaattacaga aacagaaaat tcctcaccat
    14401 ttattgcaga atatgtggat gctatacttt gagcgatcgc ttctgctgaa tcgtatgtat
    14461 ttattcgatc taaaatatca acagcagata tagaatttga aatagtattt gataataaaa
    14521 taataagatc attagttttt aatgctggaa gctgtgcagt cttgagtttg ctatcgtcat
    14581 cttcaggaaa aacttttaca atattacctc cagaaatata tatggcggaa tctgtgtcag
    14641 tagtagctaa taacgctcta tcatatgata ttaacgcagc tgatacctgc ctgttcatag
    14701 gtgaaatttc gcctgtttct tctagatttt caagatatgt aacagtagta gatatgcact
    14761 cttcgaaatc gctgaattta gtctcaacat cagattcttt atagtttttg gtaaagcgtc
    14821 tgatttctct cattaatgaa acatcagttt tactgtcatc tgtttccata ttctcatcaa
    14881 taagaaatac aaaatctgtg tcagcatatt ccattatgac ttctttacta tcataattta
    14941 aagtattaat gacttttcca ttaacatatg ctctcaagtc tgtttctttt ttgttgttgc
    15001 atataatgtt cgcagtattg agtaatattt ttgccatttt tccaccctta tttgttttgt
    15061 tcaagttgaa ttttaacatt catatagata aattgcaatc ttaaagatta
//
LOCUS       DAOSXM010000052        14746 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2111
            NODE_2569_length_14746_cov_10.326458, whole genome shotgun
            sequence.
ACCESSION   DAOSXM010000052 DAOSXM010000000
VERSION     DAOSXM010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864178
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 14746)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14746)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:24:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,261
            CDSs (total)                      :: 2,229
            Genes (coding)                    :: 2,218
            CDSs (with protein)               :: 2,218
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 3 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14746
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2569_length_14746_cov_10.326458"
                     /isolate="AMR_MDS_2111"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            65..259
                     /locus_tag="PLQ68_07350"
     CDS             65..259
                     /locus_tag="PLQ68_07350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72505.1"
                     /translation="MIISEKKEKMGFGVSEILGIGAVLLIAAAVVIPGLKEIAQDIID
                     NTKTWVGDQFNEIFDINPVT"
     gene            265..639
                     /locus_tag="PLQ68_07355"
     CDS             265..639
                     /locus_tag="PLQ68_07355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72506.1"
                     /translation="MAEKVIVNIITVLVIICLFVYCVDVISIISKNMEFRDICRGYMH
                     IAEQNSGLNDIHKAELVQDLMNNGFTEISVQTTSAAAYGSAFMFNVSASFDKSVLEGI
                     FNRNLKQYIMNFEQKIIARKIY"
     gene            639..1094
                     /locus_tag="PLQ68_07360"
     CDS             639..1094
                     /locus_tag="PLQ68_07360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72507.1"
                     /translation="MDKSKKGSAGTEIILAVALIVYILLPLFAALVQKYVIYNQAEKI
                     KQTIEMSVVSLISENSTNDFSKGLLNIMTDEDKLKDIIYLDIKCLNENMIFIPKDNIS
                     IKVYQAGNICTCGFVNSHYMISVDLTAIFDYLGTRNIHIHKHIEFPVIR"
     gene            1102..1722
                     /locus_tag="PLQ68_07365"
     CDS             1102..1722
                     /locus_tag="PLQ68_07365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72508.1"
                     /translation="MKKYKFYILAVMSAILLTVIQVLIAKAAVQKEIGFIYVVVNDIA
                     EGDMLQPEDYEKIEISGYNMQFQFNEGLIAITDLKEGSIICNEYFKEQTEIRGIHFIS
                     ITTDKSRCPTAYIKTNEKVNMFIMPDKKSLTINESAWLDNFLKNQSIAYDHDNDIGFM
                     IPDITIKHIAGLENANQSIIIEVEPPIDRLLAFLKTKCIIEFMVPN"
     gene            1792..2613
                     /locus_tag="PLQ68_07370"
     CDS             1792..2613
                     /locus_tag="PLQ68_07370"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018589297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HXK72509.1"
                     /translation="MDNYYENLLKSVEYIKSKIKKEPVLGIVLGSGLGMLADNIEDRI
                     EIDYHDIPNYPVSTIPGHAGKLIFGYLSGKYVACMAGRFHYYEGYEMEKIHIGVRTFK
                     LLGAEGVIITNAAGGINTSFSEGTIMLINDHIGFLAPSALRGPNIDEFGDRFFDMSKV
                     YDKDYLDKARMVAKRIGLDVKEGTYMFFKGPNFETPAEIRAARILGADAAGMSTVPEV
                     VACSHCNLKVLGMSVITNMAAGILDKKLNHEEVMETANKIRAVFVEYVKEIVREL"
     gene            2615..3859
                     /locus_tag="PLQ68_07375"
     CDS             2615..3859
                     /locus_tag="PLQ68_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HXK72510.1"
                     /translation="MEFDILDLSLKEIGREKIEWIKDFMPVLCMIEKDFIKSQPLKGI
                     KISMALHLEAKTAFLAVVLKNLGADIRVTGSNPLSTQDEITAALVDMGISVFAYRGVD
                     SKTYKQHLIKALSHKPHIVIDDGADLAFTLHKERPDLMENVYGGCEETTTGVVRLRAM
                     DREKVLKYPMFAINDADCKHLFDNRYGTGQSSLEGVMRATNLTLFGKKVVVAGYGWCG
                     KGIAQKAKGFGANVVVTEVDSVKALEAAMDGFDVMPMIKAAKIGDIFITSTGDIDVIT
                     KKHFMVMKNNAILCNAGHFDCEISMPELKEIAVKQEQRRNGVTGFYLSNGKVINVLGE
                     GRLVNLACADGHAAEIMDMSFALQLLTVMHVLENHDKLENKLYDVPEYIDRAVADAKL
                     KSMSIEIDKLTKKQKKYLESWD"
     gene            3861..5534
                     /locus_tag="PLQ68_07380"
     CDS             3861..5534
                     /locus_tag="PLQ68_07380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72511.1"
                     /translation="MKYKIKDRIIPCPKHISVKEGYAFFRNRTKIVLKIEETTVIKSG
                     YDLLNSLCSEGYLNPDFVITLKRLDEYYTDKKLERCENLDQAYIIKPVIVDKVYSGID
                     LMAYTDVGLYYACSTLYQAIEQISEKEIDIPYIDVTDWPSCTYRGLFLGDYIRCLDKT
                     SFLKLNALDFVYNTKSREKEDKIYADCEKYGVTVFSAVFEWDNPKEIFERLWIKEKRN
                     NLLFWIKDECEESDLHYEANRIIEAFIDYKKDCPELKAGIITNSKGNKTFDRIFALSQ
                     PEDFSISYCDKLKTYNTDVRELTDSTVSAYTKKGGLFGIYPMIAYDLKSFFLWTAPEF
                     IQFRCSEFDRINAHKVMGYTPFACSFSKFNIAAFSEWLWNPKGRNTIDFIKAYSYKNN
                     LDFDKFENMLYYLTFASWSLQRSNFVQVICDDPIDFKKIKKLPKLSQMTNDSLKAIKF
                     AEMLQDQSYIDEAKATYSALVAYSMTRKLAKLDSAKKPDQKKYLQYLQKLDDSCKTVY
                     TCLMEWAEFVKEEAGAYDAFIYETAKAFLHLPKLFDRFGSVKEESDSKN"
     gene            5531..6220
                     /locus_tag="PLQ68_07385"
     CDS             5531..6220
                     /locus_tag="PLQ68_07385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72512.1"
                     /translation="MRKLFLLITIVAIIFLLCSCNNSFEKEHTLIYDSKLINQELVDS
                     IKQNLPQSIYIEVDVYVEDDYSNMIMSKYNNIVVTESYLSDGVSVISVYDYNINKAYQ
                     YFKGDSLSEEEKTYGMELTLTNEEIEKGIDYQFEALSGIGEVEQAYLTEYEGYEAIYI
                     KSVLRYDESELVFTIYLSTKFSYPLYMSSLVDGKESVKMIVTKIDDNYLFSETFPDIP
                     EFINFVNYDDL"
     misc_binding    6267..6461
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            6510..9113
                     /gene="alaS"
                     /locus_tag="PLQ68_07390"
     CDS             6510..9113
                     /gene="alaS"
                     /locus_tag="PLQ68_07390"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009052311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HXK72513.1"
                     /translation="MNKLSLNTLREEYLNFFESKGHLRLKSFPLIPQDDPSILLINAG
                     MTPLKPYFTGTIEPPSKRITSCQKCIRTLDIDRVGITTRHGTFFEMLGNFSFGDYFKL
                     EAIQWSWEFLTKVLNLPENLLYASVYEEDDEAYEIWKDVVKLPVDHIVKLGKEDNFWE
                     HGTGPCGPSSEIYFDRGSDKGCGSVNCKVGCDCDRFVEVWNNVFTQFDKLEDGTYKPL
                     KNKNIDTGMGLERLACVVQGVDSLFEVDTIKQILDSVCDATKTKYGKDKNKDVSIRVI
                     TDHVRSAVMMISDGIIPSNEGRGYVLRRLLRRASRHGRILGNNNQFLADLTEVVINNS
                     KDAYPELSHQQEYIFKVIESEENKFVNTIESGMNILNEYLEQAQKANQDVLEGEFIFK
                     LHDTYGFPYDLTREIANERGFRLDKEGFDQAMQVQKTTAREAFKAAEGSAWKNDSGIY
                     KDLDKTEFLGYSDSVTTATVLDVFSDSDNYELVTDKTVFYAEGGGQRADTGYIRKNDA
                     VLNVLSCVNKEGIYIHKGMLEKGNFSKGDTVCCEINTDSRRATERNHTATHMLQYALK
                     EVLGNHVHQAGSYVDSSRLRFDFTHFSSMTDEQIDNVQKIVNRIILEDILVNKEIMSI
                     EEAKKKGATALFGEKYGNIVRVVSINPYSMELCGGTHLDRTGQAGLFKILSESACASG
                     IRRIEAVTGEVAFDYLISKEKTIHRLSEALKTIDLETKIDSIVKELKQTKKENDDLKN
                     KLSSSEVKDVLGTYEQVNDTKIIKAIIDNADMSQLRNLADRVKDRYDDAAAVFIGKSD
                     KPSMVIAVTKKACEKGISANKIIKEAAAYIAGSGGGREDMAQAGGKDSSNAEKALQKA
                     VDMIKEVLNNG"
     gene            9106..9453
                     /locus_tag="PLQ68_07395"
     CDS             9106..9453
                     /locus_tag="PLQ68_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006361407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HXK72514.1"
                     /translation="MDDCIFCKIVKGQIPCTKVYEDEYTFAFKDINPQAKQHILIIPK
                     EHILTGLNDVNDDNSYIIGKLFYAAKIVSEMLGIDKSGVRIINNTGKDAHQSVQHFHI
                     HVLGGEDMGEGLV"
     gene            complement(9553..9729)
                     /gene="rpsU"
                     /locus_tag="PLQ68_07400"
     CDS             complement(9553..9729)
                     /gene="rpsU"
                     /locus_tag="PLQ68_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HXK72515.1"
                     /translation="MAEVRLKENETLESALKRFKRQCAKAGVLAEVRKREHYEKPSVK
                     RKKKSEAARKRKKR"
     gene            complement(9810..10628)
                     /locus_tag="PLQ68_07405"
     CDS             complement(9810..10628)
                     /locus_tag="PLQ68_07405"
                     /inference="COORDINATES: protein motif:HMM:NF018406.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HXK72516.1"
                     /translation="MEKTKKPLYWIFIKSFTDMHGYKHFVSKNFLKAFVYMLLLTLIL
                     SVVFTTVYVISIDKTTSDIIEQLKSNTPFFAISNGELKINQDQPSKLVETSDKGTLII
                     IDTEYTGSITKYEEYETVVILAKNDIYLSNPSIRYLVDYETLFGTNDEITSETLLNII
                     SGSKSFLYVFLYITIFLFMLFNYFMYTLLIALIVGIVRRIRKKLTNFGVCLQLSVFAL
                     TLPSMLYTLWMLTSINILLGDFLIILLSYLIASKGINAYYVDLQKDITNKDKLV"
     gene            10723..>12230
                     /gene="uvrB"
                     /locus_tag="PLQ68_07410"
     CDS             10723..>12230
                     /gene="uvrB"
                     /locus_tag="PLQ68_07410"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015924912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HXK72517.1"
                     /translation="MNKADKADKFVLKSEYKPTGDQPQAIDILVDGINKGYNHQTLLG
                     VTGSGKTFTMANIIEKVQKPTLVIAHNKTLAAQLCSEFKEFFPDNAVEYFVSYYDYFQ
                     PEAYIPSTDTYIEKDLSINDEIDQLRHAATSALLQRRDVIIVASVSCIYGLGDPADYK
                     DLMLYVGVGMQKDRDDVIKRLVEIQYSRNEIDFKRGTFRAKGDSLEIAPSNSANNAYR
                     ISFFGDEIEKITQFEPLTGEIFGNVSHLAIFPASHFVSKEHKLKKALKDIQEELETRV
                     KEFENENKIVEAYRLNQRTNYDLEMIKNIGFCSGIENYSRHIDGRKEGQPPATLLSFF
                     SDDYLMFIDESHVTVPQIGGMHKGDKSRKKNLIEYGFRLPSAFDNRPLTFEEFKKRQN
                     QCIYVSATPGDYEQSISQCIAEQIIRPTGLMDPIIEVRPVKGQVDDLLAEIRETIARK
                     HRVLVTTLTKRMAERLTEYYTSLDVSVQYLHSDVETLERIQILKQLRNGTFDV"
     assembly_gap    12231..12330
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <12331..12600
                     /locus_tag="PLQ68_07415"
     CDS             <12331..12600
                     /locus_tag="PLQ68_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HXK72518.1"
                     /translation="QLEYNEKNNITPTTIVKEIRDNIHFKVMEKQGRKNSYTLQIPKT
                     EVEIIRLIDLYERQMKRYAKLQEYENAARYRDMLKELKDALNNGI"
     gene            12637..13656
                     /locus_tag="PLQ68_07420"
     CDS             12637..13656
                     /locus_tag="PLQ68_07420"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HXK72519.1"
                     /translation="MIRPMKGEGLESPDIVMKKVRVGLYGINGHQIHNQMVNNDKAVV
                     TAISMIEKDKLPKELLNGDIKFYDDFDSLIKADDVDVVSICAPKRVDQKDMSIKAMMA
                     KKHVYSEKPCAYTEADLDEILEVSKKTGMLFHEMAGSAFEEPFVSVTELIKQGRIGEV
                     VQVFAQKSYPYKTSSGRPQDDITDGGLIRWIAVHATRFVEHTTGLKITDVYAIETKHG
                     NPVKDGNLKMAASLMFSLENGAVGSIVSNYLNQNTFPTWGNEHLRVWGTKGFVEITDG
                     LTKTRLVTEDYDGPVEIKHKTVDYFDLFLDEILEGKKMPLTLEQELHPLRVVIRAQER
                     AVEVK"
     gene            13656..>14746
                     /locus_tag="PLQ68_07425"
     CDS             13656..>14746
                     /locus_tag="PLQ68_07425"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HXK72520.1"
                     /translation="MKIVVVGIGGYGEIVLNGLFANPDNLDFTIEGYVAPRPKDNDYS
                     KKLHEMGAKRYVTLEEFFETKHADLAVISSPIEFHMPQTILCLRHNTAVMCEKPVAGT
                     IQDAYEMKKAEELSKAFVGIGYQWSYSNAIINLKKDILSGKYGKPLRLKNIVLWPRTQ
                     RYFHRNSWAGKIKNDAGYWILDSVANNATAHYIHNMFFVLGSDLKKSAWPKTIQANIY
                     RANDIENYDTCVSRIITDNDVEILYYASHSTDKKVEPLLEFQFENGVIYADFNKEKCI
                     YGKIGNEIIKYGNPFDCEMKKVFDCMRTLSGDNVNYCPIEAAIPQLKTINAFSQYYNI
                     TQISSDRLGYLDAEENTTKLVYVKRLYED"
BASE COUNT         5275 a         1952 c         2900 g         4519 t
ORIGIN      
        1 tgtactgcca ttatttagcg agcttgtaca aggacttaac aatatattta attaggagga
       61 caaaatgatt attagtgaaa aaaaagaaaa aatggggttt ggtgtatcag agatacttgg
      121 aattggggcc gtgcttttga ttgctgcagc agtagttata cctggactga aagagatagc
      181 tcaagatata attgataata cgaaaacatg ggttggcgat caatttaacg aaattttcga
      241 tataaatccg gtaacttaac taacatggca gaaaaagtta tagttaacat aataacagta
      301 ttggtaatta tatgcttatt tgtatattgt gttgatgtta tatcaatcat atctaaaaac
      361 atggagttta gagatatctg ccgagggtac atgcatattg cagagcagaa ctctggtctg
      421 aatgatattc ataaggctga acttgtacaa gacttaatga ataatggctt tactgaaata
      481 tctgttcaga ccacttctgc agctgcatat ggcagtgcat ttatgtttaa cgtttctgct
      541 tcatttgaca agtcagttct tgagggaatt ttcaacagaa atcttaagca gtacataatg
      601 aatttcgaac agaaaattat tgctagaaag atatactaat ggataaaagt aaaaaaggat
      661 cagcaggaac agagattata ttagctgtag ctttaatagt ttatatattg ttacctctgt
      721 ttgctgctct ggttcaaaag tatgttatat ataatcaggc agaaaaaatt aaacagacaa
      781 tcgaaatgtc agtagtatca ttgatatcag aaaacagcac taacgatttt tcaaaggggt
      841 tacttaatat tatgacagat gaagataagt taaaagatat tatatatttg gatattaaat
      901 gtttaaatga aaatatgatt tttataccta aagataatat atcaataaag gtatatcagg
      961 ctggaaacat atgtacatgc ggttttgtca attctcatta catgatatca gttgatctta
     1021 ctgccatttt tgattactta ggtacaagga atatccatat acacaaacat atagaatttc
     1081 cagttataag gtaggaggaa tatgaaaaaa tataaatttt atatattagc agtcatgtca
     1141 gcgatcttgt tgactgttat acaagttcta atagcaaaag cagcagtaca aaaagagata
     1201 ggttttatat atgtagtagt taatgatatt gcagaaggtg atatgttaca accagaggat
     1261 tatgaaaaga tagagatatc aggatataat atgcagtttc aattcaatga agggcttata
     1321 gctatcaccg atttaaaaga aggttcaata atttgcaatg aatattttaa agaacagaca
     1381 gaaatcaggg gtattcactt tattagtatt acaacagata aatcaagatg tccgactgca
     1441 tacattaaaa cgaatgaaaa agttaatatg ttcattatgc ctgacaaaaa atctctaact
     1501 attaatgaat cagcatggtt ggataatttt ctaaaaaatc agtcgatagc atatgatcac
     1561 gataacgata tcggatttat gatacctgat attactatca agcatattgc aggacttgaa
     1621 aatgcgaatc aaagcattat tatagaggtt gaacctccaa ttgacaggtt gcttgctttt
     1681 ctgaaaacaa aatgtatcat cgagtttatg gtccctaact aatattgagt gtggaaagat
     1741 aaaatccttt atgatatact ttttttatat aatatatgtc atggaggcaa tatggataac
     1801 tattatgaga acttacttaa gtcagttgaa tatattaagt ccaagattaa aaaagaaccg
     1861 gttcttggta tagtattagg ttcagggctt ggtatgttag ctgataatat tgaggacagg
     1921 atagagatag attaccacga tattcctaat tatcctgtat caactatacc tgggcacgcc
     1981 ggtaaattaa tttttggtta tcttagtggt aagtatgtag cttgtatggc aggaagattc
     2041 cattactacg aaggctatga aatggagaaa atacatattg gcgtacgtac atttaaactt
     2101 cttggagctg aaggtgtaat tatcacaaac gcagcaggag gcattaatac atcatttagt
     2161 gaaggaacta ttatgctgat taatgatcat ataggttttt tagctccttc tgcactcaga
     2221 ggtccaaata ttgatgaatt tggagataga ttctttgata tgtctaaagt ctatgataag
     2281 gattatcttg ataaagccag aatggttgca aagagaatag gtttggatgt taaagaagga
     2341 acatatatgt tttttaaagg tccaaatttt gaaacaccag cagaaataag agctgcgaga
     2401 attttagggg cagatgcggc aggaatgtct acagtacctg aggttgttgc atgttctcat
     2461 tgtaatctaa aagttcttgg catgtcagtt ataacaaata tggcagctgg tatactggat
     2521 aaaaaattaa atcatgaaga agttatggaa acagcaaata agatccgtgc cgtttttgtc
     2581 gaatatgtaa aagaaatagt gagggaatta taatatggag ttcgatatat tggatttatc
     2641 acttaaggaa ataggacgag agaaaatcga atggataaag gattttatgc ctgttttatg
     2701 catgattgaa aaggatttta ttaaatcgca gcctttaaaa gggataaaaa tttcaatggc
     2761 gttgcatctg gaagctaaaa ctgcatttct tgctgttgtt ttaaagaact taggagctga
     2821 cataagagtt actggaagca atcctctttc tactcaggac gaaataactg cagcattagt
     2881 tgatatggga atttcagttt ttgcatatag aggagtggat tcgaaaacct acaagcagca
     2941 tcttattaag gctttatcac ataaacccca tatagtaatt gatgatggtg ctgatcttgc
     3001 atttacactt cataaagaaa gacctgatct catggaaaat gtatatggcg gttgtgagga
     3061 aacaacaaca ggagtagtaa gactaagagc aatggatcga gaaaaagttc ttaaatatcc
     3121 tatgttcgct ataaatgatg cagattgtaa acatcttttt gataacagat atggaacagg
     3181 acaatcatca cttgaaggtg taatgagagc aacgaatctt acattgttcg gaaagaaagt
     3241 tgtcgttgca ggatatggct ggtgtggtaa aggtatagct cagaaagcta aaggttttgg
     3301 ggctaatgtt gttgttacag aagtggattc agttaaggca cttgaagctg ctatggatgg
     3361 atttgatgta atgcctatga taaaagctgc aaaaattggt gatatattta taacttcaac
     3421 aggtgatatt gatgttatta caaaaaaaca ttttatggta atgaagaata atgccatttt
     3481 gtgtaatgca ggacatttcg attgtgagat ttctatgccg gaattaaaag aaattgcagt
     3541 taagcaggaa caaagaagaa atggggttac aggcttttat ctttctaatg gtaaggttat
     3601 aaatgttctt ggagaagggc ggctggttaa tcttgcttgt gctgatggac atgctgcaga
     3661 aattatggat atgagttttg cattacagct tcttacagtc atgcatgtgc ttgaaaatca
     3721 tgacaaactt gaaaataagt tatatgatgt tcctgaatat atagatagag ctgttgcaga
     3781 tgcaaaattg aaatctatga gtattgaaat agataaactg acgaagaagc agaaaaaata
     3841 cttagagagt tgggactaat atgaaataca agatcaaaga caggataata ccatgtccaa
     3901 agcacatatc agttaaggaa ggttatgctt tttttagaaa cagaacaaaa atagtattaa
     3961 aaatagaaga aactactgta attaagtcag gttatgatct tcttaattca ctatgttctg
     4021 agggatatct aaatcctgat tttgttataa cgctaaaaag acttgatgaa tattataccg
     4081 ataagaaatt agaaaggtgt gaaaatttag atcaggcata cataatcaaa cccgtaatag
     4141 ttgataaggt ttattcagga atagatttaa tggcatacac tgatgttggg ttgtattatg
     4201 cttgttcaac gctttaccag gcaatagaac agatttctga aaaagaaata gatattccat
     4261 atatagatgt taccgactgg ccatcatgta cttacagggg acttttcctt ggtgactata
     4321 tcaggtgttt ggataagact tcatttctaa agttaaatgc acttgatttt gtatacaaca
     4381 cgaagagcag ggaaaaagaa gataaaatat atgctgattg tgagaaatat ggagttactg
     4441 ttttttcagc ggtattcgaa tgggataatc caaaggagat atttgaacgt ctatggataa
     4501 aagagaaaag aaataatctg cttttctgga ttaaagacga gtgtgaagaa tcagatttgc
     4561 attatgaagc aaatagaatt attgaagctt ttattgatta caaaaaggat tgtcccgaat
     4621 taaaagccgg tattataact aattcaaagg gtaacaagac atttgataga atatttgcct
     4681 tatcacaacc tgaagatttt tctatttcct attgcgataa attgaagaca tataatacgg
     4741 atgtcagaga gcttaccgat tcaactgtat cagcttatac caaaaagggt ggtctttttg
     4801 gcatatatcc aatgattgct tacgatttaa aatcattctt tttatggact gcaccagaat
     4861 ttattcaatt tcggtgctcg gaatttgata gaattaacgc tcataaggta atgggttata
     4921 cacctttcgc gtgttcattc agcaaattca atattgctgc tttttctgaa tggttatgga
     4981 atccaaaagg aagaaatact attgatttta taaaagcata ttcttataaa aataatctgg
     5041 attttgataa gtttgaaaat atgttatatt atctgacttt tgcatcttgg tctcttcaaa
     5101 gaagtaattt tgttcaggtt atatgtgatg atccaataga ttttaagaaa attaagaaat
     5161 tgccaaaatt aagccagatg actaatgatt ctttaaaagc tattaagttt gctgaaatgc
     5221 ttcaagacca gtcatatatt gacgaagcaa aagcgacata ttctgcatta gtggcttatt
     5281 caatgacaag aaagctagct aaattggata gtgcaaagaa acctgatcaa aagaaatatc
     5341 tgcagtatct ccaaaaactt gatgactcgt gcaaaacggt atacacatgc cttatggaat
     5401 gggctgaatt tgtaaaagag gaagccggtg catatgacgc atttatatat gagacagcaa
     5461 aagcttttct acatcttcca aaattatttg acagatttgg aagtgttaaa gaagaatcgg
     5521 attcaaaaaa ttgaggaagt tatttttact gataactata gtagccataa tctttctgtt
     5581 atgctcatgc aataacagtt ttgaaaaaga gcatactctt atatacgaca gtaaacttat
     5641 taatcaggaa ctggttgatt ctattaagca aaatcttcct caaagtatat atatcgaagt
     5701 tgatgtttat gttgaggatg attattcaaa tatgattatg tctaaataca acaatattgt
     5761 tgtaaccgaa tcatatttat cagatggtgt cagcgttatt agtgtttatg attataatat
     5821 caataaagca tatcagtatt ttaaaggaga tagcctttct gaagaagaaa agacatatgg
     5881 gatggaatta actttgacaa atgaagaaat agaaaaaggc atagactacc agtttgaagc
     5941 gcttagcggt attggagaag tggaacaggc ttatttaact gaatatgagg ggtatgaagc
     6001 aatttatatt aaaagcgtat taaggtatga tgaatcggaa ttggtattta ctatctactt
     6061 gtctacgaaa ttttcatatc ctctttacat gtcaagttta gtagatggaa aggaaagtgt
     6121 taagatgatt gttacgaaaa tagatgataa ttatttattt tctgaaacgt ttcctgatat
     6181 tcctgaattt ataaattttg ttaactatga tgatttgtga gaggttagtg ttgacataca
     6241 gtattaaagt gatataatac actcaatatg gcgttatgag aaagaagagt aacataggaa
     6301 attgccaaag agagagggga tggtggaaac ccttgcaaga ttttatgtga aagctatcct
     6361 tccagctttc cgcaaaacgg aacgtatacc tgcgttaaag ggtcaagtgc tttcaaatga
     6421 aagaatttgg gtggtaccgc gtgatagctc tcgtcccttt gcaggatggg agctttttat
     6481 atacaattaa taaggacaag gtgagcaaga tgaataaatt atcgttaaat acattaagag
     6541 aagaatattt aaatttcttt gaaagtaaag ggcatctcag acttaagagt tttcctctta
     6601 ttccgcagga tgatccttca atactgctta taaacgctgg gatgactcca ttaaagccat
     6661 attttacagg aactatagag cctccttcaa aaagaataac ttcatgccag aagtgtatta
     6721 gaacactgga tattgacaga gtcggcataa caacaagaca tggcactttt tttgaaatgc
     6781 ttggtaattt ttcttttgga gattatttca aattagaagc aatccaatgg tcatgggaat
     6841 ttttaacaaa agttttgaat ttacctgaaa atttactcta tgcttcagtt tatgaagagg
     6901 atgatgaagc ttacgagata tggaaagatg ttgttaagtt acctgtagat catatagtga
     6961 agcttggcaa agaagataat ttctgggagc atggcacagg accatgtggt cctagctctg
     7021 aaatttattt tgatagagga tctgataaag gatgcggatc agttaattgt aaagtcgggt
     7081 gtgattgtga cagatttgtt gaagtgtgga ataatgtatt tactcaattt gataagcttg
     7141 aggatggaac atataagcca ttaaagaata aaaacattga cacaggaatg ggtttggaaa
     7201 gattagcatg tgtagttcag ggtgtagatt ctttatttga agttgatacg attaaacaga
     7261 ttctagattc ggtctgtgat gctactaaaa ccaaatatgg taaagataag aataaggatg
     7321 tatcaataag agtaataaca gatcatgtcc gaagcgctgt tatgatgatt tctgatggta
     7381 ttataccttc aaatgaggga agaggttatg ttttaagaag gcttcttaga agagcatcaa
     7441 gacatggcag gattttagga aataataatc agtttttagc tgatctgacc gaagttgtaa
     7501 tcaacaattc aaaagatgca tatcctgagc tctctcatca gcaggagtat attttcaagg
     7561 tcattgaaag tgaagagaat aaatttgtaa atacaattga aagcggtatg aacatattaa
     7621 atgaatatct tgagcaggct caaaaagcca atcaggatgt cttggagggt gaattcatat
     7681 tcaaacttca tgatacttac ggattcccat atgacttaac cagagagatt gcaaatgaac
     7741 gaggatttag attggataaa gaaggttttg accaagctat gcaagttcaa aagacaacag
     7801 caagagaagc atttaaagct gcagaaggat cagcatggaa aaatgattca ggtatttata
     7861 aagatttgga taagactgag tttttaggat actcagattc tgttacaact gcaactgttt
     7921 tagatgtatt ttctgattct gataattatg agcttgttac tgacaaaact gttttttatg
     7981 cagaaggtgg tggacagcgg gcagatactg gatatataag gaaaaatgat gctgttctga
     8041 atgtgctaag ttgtgttaat aaagaaggta tatatataca taaaggaatg ttggaaaaag
     8101 ggaatttttc aaaaggtgat actgtatgtt gtgaaataaa tacagatagt agaagagcaa
     8161 ctgagagaaa ccatactgca acccatatgc ttcagtatgc cttaaaggaa gtattaggca
     8221 atcatgtgca tcaagctggc tcttatgtag attcttccag gcttagattt gattttactc
     8281 atttttcatc aatgacagat gaacaaattg ataatgttca aaaaatagtt aatagaatta
     8341 ttcttgagga tattttagtt aataaagaga tcatgagcat tgaagaggca aagaagaaag
     8401 gtgcaactgc attatttggt gaaaaatatg ggaatatagt tagagtagta tctataaatc
     8461 catacagtat ggaattatgc ggtgggactc atcttgacag aaccggccag gcaggtcttt
     8521 ttaaaatatt aagcgaatca gcatgtgctt ccggtatcag aagaatagaa gcagtaactg
     8581 gagaagtagc ttttgattat ctcatttcta aggagaaaac gatacataga ttatcagaag
     8641 cacttaagac aattgattta gaaacaaaaa ttgatagtat tgtaaaggaa cttaagcaga
     8701 caaagaaaga gaatgatgat cttaagaata aactatcttc ttctgaagta aaagatgttt
     8761 taggaacata tgagcaagtt aatgatacaa agataataaa agcaattatt gataatgctg
     8821 atatgtctca attaaggaat cttgctgaca gagtcaagga ccgttatgat gatgcagctg
     8881 cagtatttat aggtaaatca gataagcctt caatggtaat agctgtaaca aagaaagcat
     8941 gtgaaaaggg aatcagtgca aataagataa ttaaagaagc agcagcttat atagcaggtt
     9001 caggaggagg aagagaagat atggctcaag caggcggtaa ggactcttct aatgctgaaa
     9061 aagctttaca aaaagctgtt gatatgatta aggaggtttt aaataatgga tgattgtatt
     9121 ttctgtaaaa tagtaaaagg acagataccc tgtacgaagg tttacgaaga tgagtatacg
     9181 tttgctttta aagatataaa ccctcaagca aaacaacaca tccttataat tcccaaagag
     9241 catatactta caggcttgaa tgatgttaac gatgataaca gttatataat tggaaaactg
     9301 ttttatgcag caaaaattgt aagtgaaatg cttggaatag ataaaagtgg ggttcgaata
     9361 atcaacaata caggaaaaga tgcccatcag tcagttcagc attttcacat acatgttctc
     9421 ggaggcgagg atatgggtga aggattagta tagatttagt attaaattct gtttaattaa
     9481 tatgattatt taaggttgta taataaagtt tttatatggg taaagaaatg ggagctttga
     9541 gctcccatgt tattatcttt tctttctttt ccttgcagct tcggatttct ttttcctttt
     9601 aacgctgggt ttttcgtaat gttctctttt tctgacttcg gctaaaacac ctgctttggc
     9661 gcactgtctt ttaaaacgct tcaaagcgct ttctagagtt tcattttctt taagcctaac
     9721 ttccgccatc tgttttcacc acccttctgt tataaggtca atcaagcctt ttgtatttta
     9781 tagttaataa attcatttgt caacagcaat cacactaatt tgtctttatt cgttatgtct
     9841 ttttgcagat caacataata tgcattgatt ccttttgatg ctattaaata tgaaagaaga
     9901 ataataagaa agtctcctaa aaggatatta atagatgtaa gcatccaaag agtatataac
     9961 attgaaggaa gcgtgagagc aaaaactgac agttgtaaac atacaccaaa gttagttagt
    10021 ttcttcctga tgcgtcttac tattccaaca ataagagcaa tcaataaagt atacataaag
    10081 taattaaaaa gcataaacag gaatatagtt atataaagaa aaacatatag gaatgatttc
    10141 gaaccagata ttatattaag caaagtttct gaagttattt catcattggt gccaaacaga
    10201 gtctcgtaat ctaccaagta tcttatactt ggattagaaa gataaatatc atttttagcc
    10261 aaaatcacaa ctgtttcata ttcttcatac ttagttatag atcctgtata ttcagtatca
    10321 atgataatca gagtgccttt gtctgatgtt tcaaccagtt tagaaggctg gtcctgattt
    10381 atttttaatt caccatttga tatcgcaaag aaaggagtat ttgattttaa ctgttcaatt
    10441 atatcagaag tagttttatc aattgaaatt acgtaaacag ttgtaaatac aactgaaaga
    10501 atcaatgtta ataatagcat atatacaaat gctttaagga agttttttga tacaaaatgc
    10561 ttatatccat gcatatctgt aaagcttttt ataaaaatcc aatataaagg tttctttgtt
    10621 ttttccattc aatcctctta attatttgtt caatttttta ttttacagca aataaaacaa
    10681 aaccatactg tatattatat acctatatga taaaatatcc atatgaataa agcagataaa
    10741 gcagataaat ttgtattaaa atcagaatat aagcccacag gagaccaacc acaagctata
    10801 gatatactgg tcgatggcat aaataagggc tataatcatc agacgctttt aggagtaaca
    10861 ggttctggta aaacttttac tatggcaaat ataatagaaa aagtacaaaa gcctactctg
    10921 gtaatagcgc ataataagac acttgcagcg cagttatgtt cagaattcaa ggagttcttt
    10981 cctgacaatg ctgttgagta ctttgtttca tattatgact attttcaacc ggaagcatat
    11041 attccttcaa ctgatacata cattgaaaag gatttatcaa ttaatgatga gattgaccag
    11101 ctaagacatg cagctacatc tgctctgttg caaagaagag atgttatcat tgttgcttcg
    11161 gtatcatgta tatatggttt gggagatcct gctgattata aagatttgat gctatatgtt
    11221 ggcgtaggaa tgcaaaagga tagagatgac gttataaaga gattagtaga aatacagtat
    11281 tcaagaaatg agattgactt taaaagaggc acattcagag caaaaggaga cagtcttgag
    11341 attgctccat caaatagcgc aaataacgca tacagaatta gtttctttgg agatgagatt
    11401 gaaaagataa cccaatttga accgcttacc ggagagatat ttggcaatgt aagtcattta
    11461 gctatatttc ctgcaagtca tttcgtatca aaagagcata agctaaaaaa agctttgaag
    11521 gatattcagg aagagttgga aacaagggtg aaggaattcg agaatgagaa taagatagtt
    11581 gaggcttatc gtcttaacca gagaactaac tatgatcttg aaatgataaa aaatataggg
    11641 ttctgctcag gtattgaaaa ctattcaaga catattgacg ggcgcaaaga aggccagcct
    11701 ccagctacac ttttatcgtt tttttctgat gattatttaa tgtttattga tgaatctcat
    11761 gtaaccgtac ctcagatagg cgggatgcat aagggtgata aatcacgtaa aaaaaatctt
    11821 atagaatacg gattcagact tccttcggca tttgataaca gacctcttac atttgaagag
    11881 tttaaaaaaa ggcagaatca atgtatctat gtttcagcta cacctggtga ttatgaacaa
    11941 agtatctcac aatgtattgc agaacagatt atcagaccaa ctggacttat ggatccgatt
    12001 atagaggtca gacctgtaaa gggtcaggtt gatgaccttt tagcagaaat aagagaaact
    12061 atagcaagaa agcatagggt gcttgtaaca actcttacta aaagaatggc agaaaggctt
    12121 actgagtatt atacaagctt agatgtatca gtgcagtatc ttcattcaga tgtagagact
    12181 cttgaaagaa ttcagatatt aaaacagtta agaaatggta cttttgatgt nnnnnnnnnn
    12241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn cagttagaat ataatgaaaa gaacaatatc
    12361 acaccaacga ctatagttaa agaaattaga gataatatac atttcaaggt aatggaaaaa
    12421 caaggaagga agaattccta taccttacag ataccgaaaa ccgaagtgga gattattagg
    12481 ttaattgact tatatgaaag acagatgaaa agatacgcga agttacagga atacgagaat
    12541 gcagcacgat acagagatat gttaaaagaa ttaaaagatg ctcttaataa tggtatttaa
    12601 tgttgaaata acactataaa tagagtaaca tatcacatga taaggcccat gaaaggagaa
    12661 gggctggaaa gccctgatat agttatgaaa aaagttagag ttggtttgta cggtattaac
    12721 ggtcatcaga tacataacca gatggttaat aatgacaaag ctgtagttac agcaatatct
    12781 atgattgaaa aggataagtt accaaaagag ttattgaatg gtgatattaa attctacgat
    12841 gattttgact ctcttataaa agcggatgat gttgatgttg tatctatatg cgctccaaaa
    12901 agagtggacc aaaaagatat gtctataaaa gcaatgatgg caaaaaaaca tgtttatagt
    12961 gaaaaaccct gcgcatacac cgaggctgat cttgatgaga ttctggaagt ttcaaaaaag
    13021 acaggtatgc tttttcatga aatggctggt tctgcatttg aggaaccttt tgtatcagtt
    13081 acagagctta taaaacaagg aagaattggt gaggttgttc aggttttcgc tcaaaaatct
    13141 tatccatata aaacatcatc aggtcgtcct caggatgata ttactgatgg tggccttatc
    13201 agatggattg cagtacatgc cacaagattt gttgaacata caacaggatt gaaaataact
    13261 gatgtgtatg caatagagac aaagcatggt aatccagtta aagacggtaa tcttaaaatg
    13321 gctgcaagtc ttatgttctc tttagagaac ggagcagtag gatctatagt ttctaattat
    13381 cttaaccaaa acacttttcc aacctgggga aatgagcacc taagagtctg gggtactaag
    13441 ggatttgtag aaataactga cggacttact aaaacaagac tagtgaccga agattatgat
    13501 ggaccagttg aaattaagca taaaacagta gactattttg atttattttt agatgaaata
    13561 ctcgaaggta aaaagatgcc attaacactt gaacaagagc ttcatccatt aagagtcgtt
    13621 attagagctc aagaaagggc agtggaggta aagtaatgaa aattgttgta gtaggaatag
    13681 gcggatacgg cgagatagta ctgaatggat tatttgcaaa tcctgataat ctggatttta
    13741 caattgaagg atatgttgca ccaagaccca aagacaatga ttattcaaaa aaacttcatg
    13801 aaatgggtgc aaaaaggtat gtgacgcttg aagagttttt tgaaacaaaa catgctgacc
    13861 ttgctgtaat atcaagcccg atagagtttc atatgcccca gacaatttta tgtcttagac
    13921 ataatacagc agttatgtgt gaaaaacctg ttgcaggaac aattcaggat gcttatgaaa
    13981 tgaaaaaagc agaggaatta tcaaaagcat ttgtgggtat aggatatcaa tggtcatatt
    14041 caaatgccat aataaatctt aaaaaagaca ttttatcagg aaaatatggt aaacctttaa
    14101 ggcttaaaaa tattgttttg tggcctagaa ctcaaagata ttttcacagg aacagttggg
    14161 caggaaaaat taagaatgat gcaggatact ggatattaga ttctgtcgca aataatgcta
    14221 ccgcacatta catacataac atgtttttcg ttcttggctc agatttgaag aaaagcgctt
    14281 ggccaaagac tatacaagca aatatttaca gagcaaatga cattgaaaat tatgatactt
    14341 gtgtatcgag aataataact gataacgatg ttgaaatatt atattatgct agccattcaa
    14401 cggataagaa agttgaacca ttattggaat tccagtttga aaatggtgta atttatgcag
    14461 atttcaataa agaaaaatgt atttatggaa aaattggaaa tgaaattata aaatatggta
    14521 acccttttga ttgtgaaatg aaaaaagtat ttgactgtat gaggacttta agtggagata
    14581 atgttaacta ttgtcctatt gaagctgcaa ttccacaatt gaaaactatc aatgctttta
    14641 gccagtatta taatattact cagatatcaa gtgatagatt gggatatctt gatgcagaag
    14701 aaaatacgac aaaacttgtt tatgttaaaa gattgtatga agacat
//
LOCUS       DAOSXM010000053        14740 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2111
            NODE_2574_length_14740_cov_12.243309, whole genome shotgun
            sequence.
ACCESSION   DAOSXM010000053 DAOSXM010000000
VERSION     DAOSXM010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864178
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 14740)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14740)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:24:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,261
            CDSs (total)                      :: 2,229
            Genes (coding)                    :: 2,218
            CDSs (with protein)               :: 2,218
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 3 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14740
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2574_length_14740_cov_12.243309"
                     /isolate="AMR_MDS_2111"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..172)
                     /locus_tag="PLQ68_07430"
     CDS             complement(<1..172)
                     /locus_tag="PLQ68_07430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019424958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HXK72521.1"
                     /translation="MSRLDEIMKRTENFRYDRFGMFIHWGIYSIPARGEWVRSRENIT
                     IEDYQKFFDEFNP"
     gene            467..1696
                     /locus_tag="PLQ68_07435"
     CDS             467..1696
                     /locus_tag="PLQ68_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXK72522.1"
                     /translation="MNYIKRDMEEVVLRLSKQFSAILITGPRQVGKTTMLKKLMEQEA
                     SGRTYVTLDDINNRMMAKTDPALFLQIHKPPIFIDEVQYAPELFPYIKIMIDNTHTPG
                     DFWMTGSQIFKLMRGVQESLAGRVALLQMLPVSQQEISGGVTVPFTTNFDMLVERQKA
                     IAKVSAPELFERIWTGGMPALVSGQYKDRAVVYSGYVNTYLERDVRDLSSAIDSLKFM
                     KFITTVAARDAQLVNYKGIADDAEIDQYTAKNWLNILETLGIIFYLHPYSNNVLKRTI
                     KTPKLYFCDTGLVCYLTRWSSAETVMNGAMSGALLENYTIVEIIKSYQNCGREPFIYY
                     YRDRDAKEIDLIIESDGELNPIEIKKTAMPDKRLTNTFSVIDKSPLKIGTSAVLCLAE
                     KFSAFDKDNLIVPIWMI"
     gene            complement(1803..3098)
                     /locus_tag="PLQ68_07440"
     CDS             complement(1803..3098)
                     /locus_tag="PLQ68_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008797688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXK72523.1"
                     /translation="MKRKISQFLIEWKNNPYRKPLILQGARQVGKTYSILEFGRNNYE
                     NVAYFNFETNPSIGKTFEESLEPGYLIPILSRIVNQTIVREKTLIFFDEVQLCERAIT
                     SLKYFCENAPEYHVIVAGSLLGLAVGREKYSFPVGKVDMKTLYPMDFEEFLLALNEES
                     LVTQIQNCYDNNTQMPHAVHEAAMNYYRQFLVVGGMPDCVDKFVETKDYVLIRHIQKT
                     ILASYLNDMSKYNRANEIKKTRLVYDNITVQLSKKNTRFQYKLIKKGGRASEFENAIE
                     WLTLSGIATRIYQVEQAVKPLENYRNIDSFKTYVSDVGLLCAKKDIVAEDVLYLSEEL
                     NDFKGGMTENFVCCQLIANGYTCYYWLSLRGAEVDFIIQRAGKIIPVEVKSAENTKAK
                     SLSVYIETYKPEYSIKISGKNFGFENGIKTIPLYATFCL"
     gene            3480..4343
                     /locus_tag="PLQ68_07445"
     CDS             3480..4343
                     /locus_tag="PLQ68_07445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72524.1"
                     /translation="MSPLKEKLLKFKQQNDIDLMGFASKERFEGLDPRYNPFSIFPEG
                     KTVILIGRRVCRGSLRGVEEATNFSDYYLFGSSWLNDEFLAISCYDLTRQIEDEGYEA
                     VPVYNNPKEIYGMGVPVAPGKLSPNVAPDFKYAAVACGLAEISYSGMIFTKQFGSRQL
                     FHMVITDAVIEQDDILDQPVCDMCMKCADACPLGAISKTEFENINICGKEMKVAKIDY
                     NLCRKCKNGCVNNRLISKAEPDRIAALCNRTCLTHLEENKLVDNRFENAFRKREAWAL
                     DYMENPVKVEG"
     gene            4353..>5446
                     /locus_tag="PLQ68_07450"
     CDS             4353..>5446
                     /locus_tag="PLQ68_07450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72525.1"
                     /translation="MKTVLTSQMIKDKANQIGIDDIGIASIEAYKDAPVLMNPKSYFP
                     EAKSVIVIAMRIPRGSYRGIEEGTHWHNYTYYSYNRLNTVIRPHLTYRIATFIEDFGW
                     EACPFYPAVPERSAREQRVPGKLPNDIVMNIRLMAAGTGIGEMGHSKVFLNRKFGPRV
                     RLGLILTDAVLEPDEMMEANSICNLCGRCVKECPGNAIPPVKDKRISVNINSNKVSWG
                     DVQMGRCTLTHHGLNNKISPFLKKSFPHMAFDVDNTDMTEEEAYRMCYPLSNANWTTY
                     DESATGRVIDYEGYLTQQYGYFALCGARGCIRACMDNLEKTKRIENLFKEPFYKKQSW
                     LMDNKPIKVRKAVNQFRDDYLDKNYPGIRKG"
     assembly_gap    5447..5546
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5547..6047
                     /locus_tag="PLQ68_07455"
     CDS             <5547..6047
                     /locus_tag="PLQ68_07455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72526.1"
                     /translation="LSKLSYKGTVEKVFMPVMKGIKESTDETVILAVLLNGKRVQIAE
                     IASDNPVRSKDSGNNLFSLISGICLAYLADEKQLLDILKANGYPEGAKDLSEVKSFVE
                     KFKLSDCDVFQKTRYNKEIETFAIPLIYEDAIFSLGIHLPGYRCDEKKKRAILQALLE
                     AKKSLS"
     gene            6114..7223
                     /locus_tag="PLQ68_07460"
     CDS             6114..7223
                     /locus_tag="PLQ68_07460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72527.1"
                     /translation="MRNFGTNAKMAYDCINRKETSGIPTGLLHIMEHSIIERIAEASP
                     GDYKKDPHGVYVKMLQNAGVSMVDQYLAENPLRLGNKGHEGITQDINIVRDGIVIDSP
                     ESVIEHIERYKIPQLLKSIEEFNPASCAQSVINAESRTQQLLGDDILKTGYSNIIFPI
                     LSYALYGYENYFMAYALYPEVIDRYFTLQGIYAKKNNQAVVDSFIQAGLPKYHRLDHD
                     MASSRGLLVDWHSLERVWYPAFIESISPAVKAEFTLLWHCDGNLMEMIPYLLEAGING
                     FQGFQYEDGMDYIKICSLRAKDKKSMVIQAGVSVTRELPLGTLSDISKQIKFLVDNGP
                     KTGLFLSFSSSCTPGAPYENIKTALDGFEYYRTHR"
     gene            7299..8702
                     /locus_tag="PLQ68_07465"
     CDS             7299..8702
                     /locus_tag="PLQ68_07465"
                     /inference="COORDINATES: protein motif:HMM:NF013300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HXK72528.1"
                     /translation="MINKKEWFGTEMELDVEGQHNSLKLIHPEILDDLGSDHPDAQWF
                     KNAGLGLFLHWCISSVDGRMDVSWGMLSRGKYYPKGTFIEEIEKFEEQEVIDDNFKMR
                     PSKYFSLAKDFNAKNYDADKWMEMAKKAGFQYAVLTTMHCDGFKLWPSDYGDFSTKDY
                     LNGRDLVREFTDACRKYGIKVGFYFTPTDWHFNKDYMSFMHYKVKEKNPELPFVDENF
                     IEKQMPSESEMEKQKKIHGIQHRNQLHELLTNYGKIDILWFDGGAMKGEVYPLEEIYK
                     LQPGVVVTIRMHGYGDFRNCEVKFAEQKPKGWWEYCTIWSKRQAWAYTNNTVYRPTVD
                     ILTELIKTKNWGGNYLLNVGPKADGEFPEAAIKGLNELSEWIAVHKESVIGSYPLPED
                     ESANVYATARDNIRYLFILPDTQNPIELKGIKKPKRVYLMSSGKDMDYSFKENSINIE
                     INKIPDLSKVEVIKIEL"
     assembly_gap    8767..8866
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8867..9188
                     /locus_tag="PLQ68_07470"
     CDS             <8867..9188
                     /locus_tag="PLQ68_07470"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF037100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HXK72529.1"
                     /translation="HMVEIPQNILEITNEYINKVRAQIPVEKAILFGSYSKGTYIADS
                     DVDIAIFSSAFEKMTRIDGLTFLLMQALGYKLDLQPQPFTMKEYEEKTGLVSEIIRTG
                     VELL"
     gene            complement(9196..12156)
                     /locus_tag="PLQ68_07475"
     CDS             complement(9196..12156)
                     /locus_tag="PLQ68_07475"
                     /inference="COORDINATES: protein motif:HMM:NF024120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004650 - polygalacturonase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28 protein"
                     /protein_id="HXK72530.1"
                     /translation="MNRLQSIYDEIDGNGISDVSNMPKTPLTSRFVSPWDTSGWYAVR
                     CNFKKGISMYSNSDDYVKQIDEGYEGADYIQTFNSKEINLIDHPELDFFVETYAEVTV
                     AIEENCKPQWLKSWIDTKRSLISSKGVKYLLYSKEYTKGAHVNIPGFETDHNHYIVIV
                     KPLSNKEKFHELPKISYSANTLPALKKRIYKSHLVEVFNDKTDGIFAEEYQPFGCCSI
                     LKDENDKKNRYLALETTDKSNIAYVTKPIGATLKYPTVFECKLNISKHAITKVLLVNS
                     QSEKGAGVLLNSDGYVYSIDKKKKICPYAAEANLTLKITIDTDNQTFDVWLNHIKQAE
                     NIPYDSDGLDTINFHLESNKSLSYVYIDNIYIYDDTQIYAVNETFEKDQLLNWASNNP
                     MSIEAYPFDKDRSLAISGRNGSAYATYSFSPADDIVSIETKVKITDESFALVPQVTDK
                     DGKTALNIAMYKNNLYASDGQSWKRIYEGLTPWMYYPNNNWFNIKVTADIRRNTYDLY
                     VDGAKRAVGFKFINKVNNLGQLAFSSEKSSKTYINRIRIYDCADFSRGVLPNAKIFDV
                     KKSPYNAKGDGKTLDTDKIQKAIDDAAYTGGTVYIHNGTFFTGSLILKPDMTLFIDRN
                     ATVLGTQDHSQYKLVAPGISLCAIRQLGRGLVYGENVSNVRITGGGTLDGNGTYRYKM
                     NDPLHNREADARPDIVYITYSNDITIENVDMKSSAFWTVVPLSSGNITMRNLNLDCMN
                     TPNRDGIDPVDCHDMTISNCNIMAGDDGLCFKTSDKIGCYNIDAYDLMIQSLASGIKF
                     GTDTYYCLKNARIRDCAIKNVNRCGVSLETVDGAAVEDVIFERLDMTDVGAPLYITTG
                     ARNRLPRGNQPIRRSYIKDVTFKDIRFEQPYPFSFTKEIRENMVIGQSKDNLIENVNF
                     INFDLKLPGGMRTIPKPPVVIDDKYPEYDRHGLSSGYAFTIKYAKNITFKNLKVTLDN
                     KDVRDEIAYFDYEE"
     gene            complement(12153..13328)
                     /locus_tag="PLQ68_07480"
     CDS             complement(12153..13328)
                     /locus_tag="PLQ68_07480"
                     /inference="COORDINATES: protein motif:HMM:NF019736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72531.1"
                     /translation="MSKAVIFGAGNIGRGLVGRILFESEYKVTFVDVNEKLIKLINSA
                     KGYPIYVTIGNEYDHRNIKNVNAIDGNDIDEVVSAAKDVSVIATAVGVNALKYIIPSI
                     AAIIKQRYIEQPDGYLNFILCENMIDVHLYVKEMLKEKLTEAEYMFMEEHIGFCQSSI
                     GCMVPAPAEELLKKNALAVAVEDYNKIYTDKDGIRGELPDIDNIIAYSPFSFYINRKL
                     FMHNMSHSVCAYLGFHKGYTYIWQSILDQEIRNKVERALEETASALSLNYNVEMRELN
                     QHAEDLVRRYGNKLLADTVARVGADPKRKLGKNDRLIGAARFCLDNSINPSTIIDILP
                     LAFSFDVENDPSSKEVSSYYKEHGLAESIKKYCQLNETEPLFGKIIAADKKNRKEQK"
     gene            complement(13424..>14740)
                     /locus_tag="PLQ68_07485"
     CDS             complement(13424..>14740)
                     /locus_tag="PLQ68_07485"
                     /inference="COORDINATES: protein motif:HMM:NF018647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HXK72532.1"
                     /translation="IRNDIVEALKAINLPVLRWPGGCFADEYHWRNGIGDKNKRKKNV
                     NTHWGGVVEDNSFGTHEFMQLCEMIGCQPYIAGNLGSGTVQEMSEWIEYITASDLSST
                     VEERIANGRKEPWKLKYFGIGNENWACGGNMRPEFYADQYRRYATYCRNYGANRLYKI
                     ACGPNSDDYNWTDKIMSIIKDNCSAISLHHYSWYTSKRATELSQDDYMKVIWKSRNMD
                     DLIKRHSTIMAKYDPEKKIKLVVDEWGTWYKTEEGTNPRFLHQQSTMTDALVAAGTLD
                     IFNSHCDIVSMANIAQTVNVLQAVILTHEENMIKTPTYHVFDLYSRHMGSRLLDSYCE
                     NKKLKSEDTVYAISHSASVNEDGLINVTLSNTSLDEDYEIDMVLFGKQIIEVDADILH
                     SESHLSHNTFDNPENVRINKYDEYKIEKNSLKIKIPPCSVVAITIR"
BASE COUNT         4561 a         2600 c         2730 g         4649 t
ORIGIN      
        1 taggattaaa ttcatcgaaa aacttctggt aatcctcaat tgttatattc tctctgcttc
       61 ttacccattc tcctctggca ggaattgaat atattcccca gtgtatgaac attccgaatc
      121 tgtcatatct gaaattttct gttcttttca ttatctcatc taatctagac attacagtgc
      181 tcctatctgt agttgatatg taaattgtaa catacttatt attaatatta attgatttta
      241 atgttatatg taaattgaaa acaccatgtg tttaagaatc ttgtttcaaa acagcatatt
      301 taaaaagaat gtagaaatat tgttctgtta attttcgtta gatataaata ttcatcaaaa
      361 agaaggtaaa tatcctaatc atggtgtctt accctttgta ttgacaattt aattaatcgg
      421 ctataatatt aacatgacca tatatttgcc gtaatggagg tgttttatga actatattaa
      481 gcgagacatg gaagaggtcg tgctacgcct aagcaaacaa ttttctgcta ttcttataac
      541 agggccacgt caggttggta aaaccaccat gttaaaaaaa ctgatggaac aggaagcatc
      601 aggccgtaca tatgtgacac ttgatgatat taataacaga atgatggcga aaacagatcc
      661 agcattattc cttcagatcc ataaaccccc gatatttatt gatgaggttc aatatgctcc
      721 cgagttattt ccatatatta aaattatgat tgataatact cacactcctg gagacttctg
      781 gatgactgga tctcagatat tcaaactgat gcgaggcgtg caggaatcac ttgcagggcg
      841 tgttgcactt ttgcaaatgc tgccagtgtc tcaacaagag atatccggtg gtgtaacagt
      901 accttttact acgaatttcg atatgttagt ggaacgacag aaagcaattg caaaagtgtc
      961 tgctccagaa ctatttgaac gtatatggac tggtggaatg cctgctttag tcagtggcca
     1021 gtataaagat cgtgcagtag tttattcggg atatgtcaac acttatctgg agcgtgatgt
     1081 acgtgattta tccagtgcaa tagactcatt gaaatttatg aaatttatta caacggttgc
     1141 agcaagggat gcgcaacttg tcaactataa aggtattgca gatgatgctg aaatagatca
     1201 atatacggct aaaaactggc tgaacatctt agaaaccctc ggtattatct tctatttaca
     1261 tccatattcc aacaatgtgc ttaagcgtac catcaagaca ccaaagctgt atttctgtga
     1321 tactggcctg gtctgttatc ttacccgctg gtcaagcgct gagactgtta tgaatggtgc
     1381 tatgagcggt gcattactgg aaaactatac aatagttgag attataaaaa gttatcaaaa
     1441 ctgtggacgt gagcccttta tttactacta tcgcgaccgg gatgcaaaag aaatcgattt
     1501 aattattgaa agtgatggcg aacttaatcc tattgaaata aaaaagactg ctatgccgga
     1561 caaacggctt actaatactt tttctgtaat tgacaaatca cctcttaaaa taggcacaag
     1621 cgcagtgctt tgcctagcag agaagttcag tgcatttgat aaagataacc tgatagttcc
     1681 gatttggatg atttaggata ttaaagcggg aatcctcggt tattcaattg ccgatgtagt
     1741 tcacggttaa agcatactgg attattcgaa aattgataac attaaaacat gaactgcaaa
     1801 acttataagc agaatgtagc atatagcgga attgttttaa tgccgttttc aaaaccgaag
     1861 ttctttcccg atatctttat tgaatattcc ggcttatagg tttcaatata aacgcttaaa
     1921 cttttagctt ttgtgttttc agccgattta acctctactg gtataatttt tcctgctcgt
     1981 tgaattataa aatccacttc agcacctctt aaagacaacc agtaatagca tgtataccca
     2041 tttgcaatga gctggcagca gacgaaattt tcggtcatgc cgcccttgaa gtcatttagt
     2101 tcttcagaga gatataggac atcctctgca acgatatctt tttttgcgca aagcaggccg
     2161 acatcagaaa catatgtctt gaatgaatca atgttacgat agttttccaa aggtttaaca
     2221 gcttgttcaa cctgatatat tcttgtagca ataccggaca atgttaacca ctcgattgca
     2281 ttttcaaact cagatgcccg acctcctttt tttatcagct tatactgaaa acgggtattt
     2341 ttctttgaca actgcacggt tatgttgtca tacacgagac gcgtcttttt gatttcattt
     2401 gctcgattgt atttgctcat atcattgaga tagctggcaa ggatcgtctt ttgtatatgg
     2461 cggatcaaga cataatcctt tgtttcaaca aacttgtcaa cgcaatccgg cataccgcct
     2521 accactagaa attgtctgta atagttcata gcagcctcat gtacggcatg tggcatctgt
     2581 gtgttgttat cgtaacaatt ttgtatttgg gtaacaaggc tttcttcatt gagcgcgagt
     2641 aagaattctt caaaatccat aggataaaga gttttcatat cgactttgcc tacagggaat
     2701 gaatactttt ctctacccac cgctaagcct aaaagacttc ctgcaacaat cacatgatat
     2761 tcgggagcat tttcacagaa atatttcaac gatgtaattg ctcgttcaca cagctggact
     2821 tcatcaaaga agataagtgt tttctcccgc acgatcgtct gattgacaat cctagataat
     2881 atcggtatta agtagccagg ttccaggctt tcttcaaatg ttttaccaat agaaggattc
     2941 gtctcaaaat taaaatatgc cacattctcg tagttgtttc ggccaaattc aagtatcgaa
     3001 tatgttttcc ctacttgtct tgcgccttgt aatatcaatg gtttacgata tggattgttt
     3061 ttccattcga ttaagaactg tgatatttta cgcttcaatt aaagcacctc ctttatttgt
     3121 atgaagagac tataacacat attcatgtat atttacaata aataatttgt gatattaaca
     3181 caaaaacgca tgaataattc tcaatatcta cattatatgc ggtttaatgt tcgtattacc
     3241 gttataactc ccagtttcaa caaacaataa gcgaaaaatt ttgcattaga tggttttgct
     3301 gtaatagata aatctccact caaatttgga tgatttaata gttatataga aaagaagtaa
     3361 tagtatttta gttaaatgtg actatatctg tttgacaaca ccttcttact gctttaaaat
     3421 attagtatat ttcattaata aagaatacat ttcattaata cggaaataag gagggtaata
     3481 tgtcgccatt gaaagaaaaa ttgttgaagt tcaaacagca aaatgatata gaccttatgg
     3541 ggtttgcatc aaaagaaaga tttgaaggat tggatcctcg ttacaatcct ttttccatat
     3601 ttccagaggg caaaactgtc atcttaattg gaagaagagt atgcagaggt tcattaagag
     3661 gagtagaaga agctactaac tttagtgact attatctgtt tggaagttct tggctaaatg
     3721 acgagttctt agcaatatcc tgttatgacc ttacaagaca gattgaagat gaaggatatg
     3781 aagctgtacc tgtgtacaat aatccaaagg aaatatatgg catgggagtc cctgtagcac
     3841 caggaaagtt atcaccgaat gtagcgcctg attttaaata tgcagcagta gcatgcggat
     3901 tagcggaaat atcttattca ggaatgatat tcacaaaaca gttcggttca cgacagctat
     3961 tccacatggt tattacagat gcggttatag agcaggacga tatcctcgac cagcctgtgt
     4021 gtgatatgtg catgaaatgc gctgatgcgt gtccgctggg agctatatcc aaaacagaat
     4081 ttgagaacat taatatatgc ggaaaagaaa tgaaagttgc aaagattgac tataacctgt
     4141 gcagaaaatg taaaaacggc tgtgtaaaca acaggttaat atccaaagca gagccagaca
     4201 ggatagcagc attatgcaac aggacatgcc tgacacactt agaagaaaac aaacttgttg
     4261 ataacaggtt tgaaaatgct tttagaaaaa gagaagcatg ggctttagac tatatggaaa
     4321 acccggtaaa ggtagaagga taggtaaatg tcatgaaaac agtactgaca tcacagatga
     4381 ttaaagataa agctaatcag ataggaatag atgatatagg gatagcatct atagaagcat
     4441 acaaagatgc tcctgttctt atgaatccga aaagctactt ccctgaagcc aaatctgtaa
     4501 tagtcattgc catgaggatt ccaagaggct cttaccgagg aatagaagaa ggaactcact
     4561 ggcataacta cacatattat tcatataaca ggcttaatac tgtaatacgt cctcatctta
     4621 cataccgaat agctacattt attgaggact tcggctggga agcctgccct ttttatccag
     4681 cggttcccga aagaagcgca agagagcaga gggttccggg aaaactgcct aatgatatag
     4741 ttatgaatat acggcttatg gcagcaggta ctggaatagg ggaaatggga cattcaaagg
     4801 tatttctaaa cagaaagttc ggtccccgtg taagattagg cttgatactg acagacgcag
     4861 tactggaacc tgatgaaatg atggaagcta acagcatatg caatctttgc ggaagatgcg
     4921 taaaagagtg tccgggtaat gccataccgc cagtgaaaga taaaaggata agcgtaaaca
     4981 taaacagcaa caaggtttca tggggcgatg tgcagatggg aagatgcaca ctaacacatc
     5041 atgggcttaa taacaagatt tcaccgtttc tgaaaaaatc atttcctcat atggcatttg
     5101 atgtagacaa caccgatatg acagaagaag aagcatacag gatgtgttat cctctttcaa
     5161 atgcaaactg gactacatat gatgaatcag caactggaag agtaatagat tatgaaggtt
     5221 acttgacaca gcagtacgga tacttcgcat tatgcggagc aagaggctgt ataagggcat
     5281 gtatggataa tcttgagaaa accaaaagaa tagaaaacct gtttaaggag cctttctata
     5341 aaaagcaatc ctggttaatg gataacaagc ctataaaggt aagaaaagcc gtaaaccagt
     5401 tcagagatga ttatctggat aagaattatc caggtataag aaaaggnnnn nnnnnnnnnn
     5461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5521 nnnnnnnnnn nnnnnnnnnn nnnnnnctta gtaaactttc gtacaaagga acagtagaga
     5581 aagtctttat gcctgttatg aaaggtataa aggaaagtac agacgagact gttattttag
     5641 ctgtccttct taacggaaaa agggttcaga tagcggaaat agcctctgat aatcctgtca
     5701 gaagcaagga ctcaggtaat aatcttttca gtctcataag cgggatatgc cttgcttatc
     5761 tggctgatga aaagcagctt ttggatatac tgaaagcaaa cgggtatcct gagggagcaa
     5821 aggatttatc cgaagttaaa tcatttgttg aaaagttcaa actttctgac tgtgatgtat
     5881 ttcaaaagac aagatataac aaggagatag agacatttgc aattccattg atatacgaag
     5941 acgctatatt ttctttaggt attcatcttc caggttacag atgtgatgag aagaaaaaaa
     6001 gagcaatctt acaagcgctt ttagaagcaa aaaaaagtct gtcataaatc tcttgactgc
     6061 cccttaaagc aggcatagta ttaaaacaaa gagaatgctt taggggcagg tatatgagaa
     6121 atttcggaac taatgcaaag atggcgtatg actgtataaa cagaaaagaa acgtccggta
     6181 tacctaccgg gcttttacat attatggagc attccataat agagcgtatt gcagaagctt
     6241 caccgggaga ctataagaaa gatccgcacg gagtgtatgt gaagatgctt cagaatgccg
     6301 gggtcagtat ggtagaccag tatcttgcag agaatccttt acgattagga aataaaggcc
     6361 atgagggcat aacacaggat attaatattg ttcgggatgg gatcgtaatt gattcccctg
     6421 aatctgtgat tgagcatata gaaaggtaca agatacctca gcttttaaaa tccattgaag
     6481 aatttaatcc tgcatcatgc gcccagtcag tcataaatgc agaatccaga acacagcagc
     6541 ttttaggtga tgatatattg aaaacaggtt atagcaatat catattcccg attctttctt
     6601 atgcattata tggttatgag aactatttca tggcatatgc tctttatccg gaagtaatag
     6661 atagatattt tactcttcag ggtatatatg ccaaaaagaa taatcaggcc gtggtggatt
     6721 cgtttataca agcaggcctt ccaaagtacc acagactgga ccatgatatg gcaagctcaa
     6781 gaggcctttt agtagactgg cattctttgg agcgcgtatg gtatcctgca ttcatagaat
     6841 ccatatcccc ggcagttaaa gcagagttca ctcttttatg gcactgtgac gggaatctta
     6901 tggaaatgat accatatctt ttggaagcgg gaataaacgg gtttcaagga ttccagtatg
     6961 aagacggaat ggactatatc aaaatatgca gtttgcgtgc aaaagataaa aaaagcatgg
     7021 tcatacaagc gggagtatca gtaacaaggg aactgccttt aggaacgctt tctgacatat
     7081 caaaacagat aaaattttta gtggataatg ggcctaaaac agggctgttc ttgtcctttt
     7141 ctagttcctg cactcctgga gcgccttatg agaacataaa aacagcgctt gacggctttg
     7201 aatactacag gactcataga taaaatagaa tcattttgtg tatagtgtat agtgtatatg
     7261 catataaaac ttaatataac taaatcagga ggggtaagat gattaataaa aaggagtggt
     7321 ttggtaccga gatggagctg gatgttgaag gtcagcataa ctcactgaag cttatacatc
     7381 ctgaaatact ggatgactta ggttcggacc atcctgacgc ccagtggttt aaaaatgcgg
     7441 gattaggtct ttttctgcat tggtgcatat catcagtcga cggcaggatg gatgtatcat
     7501 ggggaatgct ctcaagaggt aaatattatc caaaaggcac atttattgaa gaaatagaaa
     7561 agtttgaaga gcaggaagta attgatgaca acttcaaaat gagaccttca aaatactttt
     7621 ctttagctaa ggattttaat gcaaagaact atgatgctga caaatggatg gaaatggcaa
     7681 aaaaagccgg attccagtat gctgtcctta ctaccatgca ctgtgatgga tttaaattat
     7741 ggcctagcga ttatggtgat ttttcaacaa aggattattt aaacgggcgt gaccttgtaa
     7801 gagaatttac tgacgcgtgc agaaaatacg gaataaaagt aggcttctat ttcaccccca
     7861 cagactggca ttttaacaaa gactatatgt cttttatgca ctataaggta aaagaaaaga
     7921 atcctgagct tccctttgtg gatgaaaact tcatagaaaa acaaatgcca tcagaatctg
     7981 agatggaaaa gcagaaaaaa atacatggaa ttcaacacag aaaccagctt catgaacttc
     8041 ttaccaacta cggtaaaata gatatacttt ggtttgacgg gggagctatg aaaggagaag
     8101 tttacccatt ggaagagata tataagctgc agcctggagt agtagtgact ataaggatgc
     8161 atggatatgg ggattttcga aactgcgagg tgaaatttgc agaacagaaa ccaaaaggct
     8221 ggtgggagta ctgcacgata tggagtaaaa ggcaggcatg ggcatacacg aataacactg
     8281 tatacaggcc tactgtagat atactgactg agcttattaa aacaaagaac tggggtggga
     8341 attatctgtt gaatgtaggg cccaaagcag acggggaatt tcctgaagca gcaattaaag
     8401 gacttaatga actttctgaa tggatagctg tccataaaga aagtgtaata ggaagctatc
     8461 ctttaccaga agatgagtct gcaaatgtgt atgcgactgc aagggataat atcaggtacc
     8521 ttttcatact tcctgatacg caaaatccta tagaattaaa aggtattaaa aaaccgaaaa
     8581 gagtatatct tatgagttca ggtaaagata tggattactc attcaaagag aatagtatta
     8641 atattgaaat taataaaata cccgatttat caaaagttga ggtaataaag attgagttat
     8701 aaattgaaca gtcgtaatac taacaatagg attcaattat atagataaat aatatgatat
     8761 aataatnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnntcat atggttgaaa
     8881 tccctcaaaa catacttgaa ataacaaatg aatatataaa taaagtaaga gcacagattc
     8941 ctgttgagaa agctatttta tttggctctt attcaaaagg tacatatata gcagatagtg
     9001 atgttgatat tgctatcttt tcatcagctt ttgaaaaaat gacccgcatt gatgggttga
     9061 cttttctttt aatgcaagct ttagggtata aactggattt acagccacaa cctttcacta
     9121 tgaaagagta tgaagaaaaa accgggctgg tatctgagat aatcaggact ggagtcgaat
     9181 tgctttaatt attacctact cctcataatc aaaatatgct atctcgtctc ttacatcctt
     9241 attatcaaga gttactttaa ggtttttaaa ggtaatgttt tttgcatatt taattgtaaa
     9301 tgcataacct gatgataatc catgtctgtc atattcagga tacttgtcat caataacaac
     9361 aggtggtttt ggaatagttc tcattccgcc tggcagttta agatcgaaat taatgaaatt
     9421 tacgttttct atcagattat cctttgactg tcctataacc atattctcac gtatttcttt
     9481 tgtaaaacta aatgggtaag gctgttcaaa acgaatatct ttaaaggtaa catctttgat
     9541 ataacttctt cttatgggct gattacctct tggaagcctg ttccttgctc ctgtagtaat
     9601 atataatgga gctcctacat cagtcatgtc cagtctttca aatatgacat cttcaactgc
     9661 tgctccgtct acagtttcca aggacactcc gcatctgttt acgtttttaa tcgcacagtc
     9721 tcttattctt gcatttttga gacagtaata tgtgtcggtt ccaaatttta taccgcttgc
     9781 aagactctgt atcataaggt catatgcatc tatgttatag caacctattt tatccgaggt
     9841 tttaaaacac aatccgtcat ctcctgccat tatattgcag tttgatatgg tcatatcatg
     9901 gcagtccaca ggatcaattc catcacggtt tggtgtgttc atgcagtcaa gattgagatt
     9961 tctcatggtt atgtttcctg aagacagagg aacaactgtc cagaaagcag aactcttcat
    10021 gtccacattc tctatagtaa tatcatttga ataggttatg taaactatgt ctggtcttgc
    10081 gtcggcttct ctgttgtgta aaggatcatt cattttgtaa cgatatgttc cattaccatc
    10141 caaagttccg ccgcccgtta ttcttacatt agaaacattt tctccatata caagacctct
    10201 tccgagctgt cttatagcgc acagtgaaat tcctggtgct accagcttat actgactgtg
    10261 atcctgtgtt ccaagcacag ttgcgtttct atcaataaac agagtcatgt ctggtttaag
    10321 gattaaactg cctgtaaaga aggtaccatt gtgtatatat actgttccac cggtatatgc
    10381 agcatcatca attgcttttt gtattttatc tgtatctaat gtcttgccgt caccttttgc
    10441 gttatatggc gatttcttaa catcaaatat cttagcatta ggaagtactc ctcttgaaaa
    10501 atcagcacag tcgtagattc ttatacggtt aatgtatgtc ttagatgatt tttcggaact
    10561 gaaagctaac tgtcctaaat tattaacctt gtttataaac ttaaatccta cagctctttt
    10621 agcaccatca acataaaggt catatgtgtt tcttcttata tctgctgtaa cctttatatt
    10681 aaaccagttg ttattaggat aatacatcca tggagtaaga ccttcatata ttcttttcca
    10741 tgactggcca tctgatgcat aaagattgtt tttgtacatt gctatattta atgctgtttt
    10801 tccatctttg tctgttacct gtggaacaag agcaaaggat tcgtcggtta tttttacttt
    10861 tgtttctata gagactatat cgtctgcagg gctaaacgaa tatgtggcat atgcagatcc
    10921 gtttcttccg gaaatagcca aacttctgtc tttatcaaaa ggatatgctt caatggacat
    10981 aggattattt gatgcccagt ttaaaagctg gtctttttca aaggtctcgt tgacagcata
    11041 aatttgcgtg tcatcatata tgtatatgtt atcaatatat acatatgata agctcttatt
    11101 gctttccaga tgaaagttaa tagtgtctaa accatctgag tcataaggta tattttcagc
    11161 ctgttttata tgattaagcc agacatcaaa agtttgattg tctgtatcta ttgttatttt
    11221 caaagtaaga tttgcttcag cagcatacgg gcatattttc ttttttttgt ctatactgta
    11281 aacatagcca tcactattga gcaatactcc tgcacctttt tcgctttgtg aattaactaa
    11341 gagtactttt gttattgcgt gttttgatat gttcagtttg cattcaaaaa cagtaggata
    11401 ttttaaagta gcaccaatag gctttgtaac gtaagcaata ttgcttttat ctgttgtttc
    11461 taaagctaaa tatctgtttt ttttgtcatt ttcatctttt aatatgctac aacagccaaa
    11521 tggttggtat tcttcagcaa atataccatc ggtcttgtca ttgaatactt caacaagatg
    11581 gcttttatat atacgctttt tcaaagcagg cagggtattt gcagaataac tgatttttgg
    11641 cagttcatga aatttctctt tgttggataa tggtttaact attactatgt agtgattatg
    11701 atcagtttca aaaccaggta tatttacatg tgcgccttta gtatattctt ttgaataaag
    11761 taaatatttc actccctttg aagaaataag gcttctttta gtgtctatcc agcttttaag
    11821 ccattgtggc ttgcaatttt cttctatagc aacagtaact tcggcatatg tttcgacaaa
    11881 gaaatccagt tcgggatgat caataaggtt tatttctttt gagttaaatg tctggatata
    11941 gtctgctcct tcatatccct catcgatttg tttcacataa tcgtcactgt ttgagtacat
    12001 cgatatgcct tttttgaaat tacatcttac tgcataccag cctgatgtgt cccatgggga
    12061 tacaaatctg cttgttaaag gtgttttagg catatttgat acatcactta taccgtttcc
    12121 atcaatctcg tcgtaaatgc tttgaagtct gttcatttct gctctttcct gtttttcttg
    12181 tcggctgcaa taattttccc aaaaagcggt tcggtttcgt tgagctgaca gtattttttt
    12241 atcgattctg ctaatccatg ttccttatag tatgagctta cctcttttga tgaaggatca
    12301 ttttccacgt caaaagaaaa tgccaaagga agaatatcta ttattgttga agggtttata
    12361 ctgttatcaa gacagaacct ggctgcacct attaagcggt cgttctttcc aagttttctt
    12421 tttgggtcag caccaactct tgcaactgta tctgcaagaa gcttgtttcc atatcttctt
    12481 acaaggtcct cagcatgttg attgagttct ctcatttcca cgttatagtt cagtgaaagg
    12541 gcagaagcag tttcttctag tgctctttca actttgtttc gtatttcctg gtccagaatt
    12601 gactgccaga tatatgtata tcctttatgg aaaccaaggt atgcgcaaac ggaatgactc
    12661 atattatgca taaacaattt acggttgata tagaaggaaa aaggtgaata tgcgataata
    12721 ttatcaatat ctggtaattc tccccttatt ccatctttgt ctgtatatat tttattgtag
    12781 tcttctactg caactgccaa agcgttcttt tttaaaagtt cttcagcagg tgcaggcacc
    12841 atacatccta ttgatgactg acagaaacct atatgttctt ccataaacat atattcagct
    12901 tcagtaagct tttctttaag catttcttta acatacaggt gaacatcaat catgttttca
    12961 cataatatga agttaagata tccatcaggc tgttcaatat atctttgctt tatgatggct
    13021 gctatggaag gtataatata tttcaatgca tttaccccga cagcagtagc aataacagac
    13081 acatcttttg cagctgatac tacttcatct atatcattac catcaatggc attaacattt
    13141 ttaatattcc tatggtcata ttcatttccg attgtaacat atatcggata tcctttagct
    13201 gaatttataa gcttgataag tttttcgttg acatcaacga atgtaacttt gtattcagat
    13261 tcaaaaagta tccggccaac aagacctctg cctatgttgc ctgccccgaa tattacagct
    13321 ttactcataa tctctacctg ctttccccga gataatctac cacaacgaaa ggctataatc
    13381 aagaataagg cgaaatgaaa attataaaag ttccgataat ttcctatctg atggtaattg
    13441 ctaccactga acaaggcgga atttttatct tcaaactgtt cttctcaatt ttgtattcat
    13501 catatttgtt aattctgaca ttttcaggat tatcaaatgt attatgtgat agatgtgatt
    13561 cggaatgcaa aatatccgca tcaacttcta ttatctgttt tccaaacaac accatatcaa
    13621 tttcataatc ttcatccaaa cttgtattag aaagtgttac attaattaat ccatcttcat
    13681 taacagaagc ggaatgagat attgcataaa cggtatcctc acttttaagc tttttgtttt
    13741 cgcaataact atctaaaaga cggcttccca tgtggcggct gtataaatcg aaaacatgat
    13801 atgtgggagt cttaatcatg ttttcttcat gggttagtat tactgcctgt aaaacattga
    13861 cggtctgggc aatattagcc atgctgacaa tatcacagtg actgttaaat atatccagag
    13921 ttcccgctgc cactaaggca tcagtcatgg tgctctgctg gtgtaagaat cttggatttg
    13981 ttccttcttc tgttttgtac catgtgcccc attcatcaac gacaagcttg atcttttttt
    14041 cagggtcata cttagccatt attgtgctgt gacgttttat gaggtcatcc atatttcgag
    14101 atttccatat aaccttcata taatcatctt gggataattc agttgctctt ttagatgtat
    14161 accatgaata atggtgtaag gaaatagctg aacagttatc cttgataata ctcataattt
    14221 tatctgtcca gttataatca tcagaattgg gtccgcatgc tatcttatac aatctgttag
    14281 ctccatagtt tctgcagtaa gtcgcatatc ttctgtactg gtctgcataa aattcaggac
    14341 gcatgttgcc accgcatgcc cagttttcat tacctatacc gaaatactta agcttccatg
    14401 gctcttttct tccattagct attctttcct cgactgttga ggataaatct gatgcggtta
    14461 tgtactcaat ccattcagac atttcctgta ctgtcccgct accaagattt cctgcaatat
    14521 acggctgaca gccaatcatt tcacataact gcataaactc atgagtacca aaactgttgt
    14581 cttcaacaac tccgccccag tgggtgttaa catttttctt tcttttattt ttatcaccaa
    14641 taccgtttct ccagtgatat tcatctgcaa aacatccgcc aggccatctt agaactggta
    14701 gatttattgc tttgagcgct tcaacaatat catttcttat
//
LOCUS       DAOSXM010000054        14492 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2111
            NODE_2638_length_14492_cov_9.868324, whole genome shotgun sequence.
ACCESSION   DAOSXM010000054 DAOSXM010000000
VERSION     DAOSXM010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864178
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 14492)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14492)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:24:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,261
            CDSs (total)                      :: 2,229
            Genes (coding)                    :: 2,218
            CDSs (with protein)               :: 2,218
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 3 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14492
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2638_length_14492_cov_9.868324"
                     /isolate="AMR_MDS_2111"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..308
                     /locus_tag="PLQ68_07490"
     CDS             <1..308
                     /locus_tag="PLQ68_07490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72533.1"
                     /translation="MITFSGTCVSIGKKFELDFKLLNTAYESWMTLDEGDKVKTSFEI
                     TEGSVDIYVRHEDNSIIYQGNDVESGDFIIEISKSDKYYFEVIGSHAAGYVHFNKLP"
     gene            complement(317..493)
                     /locus_tag="PLQ68_07495"
     CDS             complement(317..493)
                     /locus_tag="PLQ68_07495"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HXK72534.1"
                     /translation="MGRVTFSAGIYCFSGKEDLKVLIDKADTALYVSKKNGRNRITVY
                     NPEMGQLDKTDIVD"
     gene            complement(845..1231)
                     /locus_tag="PLQ68_07500"
     CDS             complement(845..1231)
                     /locus_tag="PLQ68_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010075406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXK72535.1"
                     /translation="MKRSLMQVYVNSSDKAVVFYQKAFDATLLCAYSYDDGTYMHSEL
                     DIEGQVLAVSERNDENSQNSKEAITGNTMQFCLHYGQGNEDKVRRAYEVLKDDAQIIY
                     PLSECEYSPLMTDLIDKYGVRWCIFI"
     gene            complement(1316..2437)
                     /locus_tag="PLQ68_07505"
     CDS             complement(1316..2437)
                     /locus_tag="PLQ68_07505"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HXK72536.1"
                     /translation="MLYILIMLPLKKPKRRSLIFAILGSIIITGINAVLIQFIGISFY
                     IRFYFLTLTLPYILLGLYFSAFKAAKFIFIILTTQLFGTVAIINGLLASYIFYGENNP
                     LIDTAARVLTYLIFLPVVIKLIRPTYLKMTEVLKKGWWMLNSVLIMSYALSYFILFVP
                     YAVFYRPVYFYHAYIGTILSLLIYAIIFYLFLEIQTKTKVERDKQLLSTQVTSLEKET
                     AAISTIAYKDSLTGLNNRYSLYKQLNEYINNKQEFLVVFIDLDNLKMINDTYDHSTGD
                     TYLKQFAKALQNAVGKQGDVYRFAGDEFICLITNISDGFDSEDFKKSVSNEMVMEKPY
                     YGISLGFAHYPSDGQDADDLIKYADREMYLEKRAKRKND"
     gene            complement(2653..3264)
                     /locus_tag="PLQ68_07510"
     CDS             complement(2653..3264)
                     /locus_tag="PLQ68_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003511940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HXK72537.1"
                     /translation="MKKKSGERTLLASVILSSPGPIVVGLGLLLGRSSTQFVDFIRRT
                     AELAAIIVSWVVFRYLSKNEGTDIKRKEKLEHTANLSVGIAMCVSGIAMIFVALFRSN
                     AEKGNVVPGLIIALLGVVVNFLFWIRYSKLNRENPDAILSVQSKLYFAKLLVDLCVTV
                     TLVIIVIAPSGSLTYYVDLIGSILVSVYLILNGILTILGKIIK"
     gene            complement(3261..3980)
                     /locus_tag="PLQ68_07515"
     CDS             complement(3261..3980)
                     /locus_tag="PLQ68_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003511939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HXK72538.1"
                     /translation="MTEFFIGFGILLGYFIVCATIALICKRFVKMPNEVFRKLLHMIL
                     ICSIFVLTYAFSTWWISVAASVLFALIVFPILLFAERIPGYSNLLTERKKGEIKKSLL
                     IVFLMFSILISVCWGLLNLKYLVIASVFAWGFGDAAAALVGKRFGQYYIEGKLVEGKK
                     SLEGTFAMFVVSFISVFIILIAYSSLNWYSYLLIAIITAAVSAVVELYTKGGMDTLTC
                     PLAAAFTMILLLYLQGSVIYL"
     gene            complement(3973..4416)
                     /locus_tag="PLQ68_07520"
     CDS             complement(3973..4416)
                     /locus_tag="PLQ68_07520"
                     /inference="COORDINATES: protein motif:HMM:NF014080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HXK72539.1"
                     /translation="MLLIRKMWLDMKGVIRAARKIINAQLEPFGLTGTEGDLLFLLVT
                     GSDNLQQEQLAEQLDIGKAVVSRAVDSLVFKGYVSRTRSNTDRRAYTIILTDKALKIS
                     KSITEVYDNLYSLARKGIADDDFINIETLLSRISKNLQHCGDCND"
     gene            complement(4600..6096)
                     /locus_tag="PLQ68_07525"
     CDS             complement(4600..6096)
                     /locus_tag="PLQ68_07525"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HXK72540.1"
                     /translation="MNTKIVKFITILLVMILMIPYISVNSQEDQYAWILINSVNYDNE
                     DKWAVANENESYNNEYSCSTGQYSVKTIYTGRTEDWRNPPKIYGEAIRLSADFTGVPE
                     KIIPGEEVTITINMTASENSLSYFGFSASATVDFDRYDLGPGSRTQGAILFKDKDGQH
                     HFEITLQEGYMDVTATVSGKLGSGKEEGELIALRYHFYRGISMGTNYVYQWQKLSNQI
                     QPTQPVVNRDGMYNQTPKNGVLPVNPKPAGDFTGAEIADYSGNVTVWIWVERSNPYYW
                     QEHDEEYILSGKDGYWYSWKPGLEQLPLNAIVQTDYDSEAVVLMNNMNTLILSSNSMI
                     TLSGQSHTVNLLTLINGKAWFNIRQIASNSHISIKMSQATVTIKGTTFVLEEDMQTST
                     VKVFEGEVEMLQYSDNSTSIISAGNLLSAIEGKSSDIQKFDVNAELDIWDNNVNEITV
                     SAMKGSKKTGIDITTILFIAGAVVVLASIVVVISVNKKNSKNQTNRPY"
     gene            complement(6109..6786)
                     /locus_tag="PLQ68_07530"
     CDS             complement(6109..6786)
                     /locus_tag="PLQ68_07530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HXK72541.1"
                     /translation="MYCQNCNTKIENDMRFCVNCGAELNIGSGSEPGYSSGNRIGFSD
                     RINDPRVHQIVKKMKSSGTVFTLILSVIALIVFTILGLAEVGGFEFPSSLYIGLAFAG
                     LLVILALFQLRKSKKDITWDGTIIDKRIKKPTYAERKQGHLQNQYYIDIQRDDGKVES
                     MVYTEQLFDYYKIGERVRHHGKMPASHILEKYDKSNETIVYCIACSSKNNMSDDLCYR
                     CKCPLMN"
     gene            complement(6807..7493)
                     /locus_tag="PLQ68_07535"
     CDS             complement(6807..7493)
                     /locus_tag="PLQ68_07535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72542.1"
                     /translation="MKKYFIIALTIIIALTVLAGCTEKPVKTLSSSLTSQTPQSTHQQ
                     EGEEQVKHQNYYIYITKNSWDTVSWDNVYTPHLAFSTMIKELGEIPVSENGNFSIEFS
                     GLLFENEVAKVNVKELNIFGTIQDIQQLAGVTTGTLIGQGTLNVNLNYVIHQKDWPRP
                     LVEDYDVTVEGDMQIFSERDGQRIRITSVDLLKANGKSTYDDNSQTDTIDIDYDYMLS
                     LVFNIVVKDE"
     gene            complement(7495..8019)
                     /locus_tag="PLQ68_07540"
     CDS             complement(7495..8019)
                     /locus_tag="PLQ68_07540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72543.1"
                     /translation="MKRIKVRLFICISILAPIIFFLFFIITKFESSSQFKTIILMTLS
                     FLAVISLVLTIKQFQNLKQAKLIIENKIMSIQPVLIKTQDGQSDEQSIDMTISCFGIL
                     LNTKVIRFNQGKVHLKTVELYHDYISITYGSNKKENQIRLLYKKIDQNQINDISKKFH
                     FETGIVPKILYQED"
     gene            complement(8153..9577)
                     /locus_tag="PLQ68_07545"
     CDS             complement(8153..9577)
                     /locus_tag="PLQ68_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR family transcriptional regulator"
                     /protein_id="HXK72544.1"
                     /translation="MKIIKPVINERRLTILVFSLFFAWLLSIPFEGEILYTLIEITKI
                     SYGTFLIFGSIFFHFIGLIVSSLIIKTKSRARLLMLISIIYCMIATTVFFMTPSVLWI
                     IAVLSGSFLTGSCVAGWAFFLKNSTHRSERIKIIADSLIGSNVLMILINMTAVHVSVQ
                     TGLIFSIGVLILALLFASMLPKKDDEKKDNEDISIPGQSNVIKPLALLCLFIVILTIS
                     SGLMYQVQGPAYSHLRGLTSWYWAIPYIIALLVMRNLPKKFNRTYILYVAIAMIGLSY
                     IAFMIFEHNVISYFIVNTLMLGACGIYDLFWWSILGEMLDWHRNPSRILGIGLSSNVL
                     GLLVGGVVGRIMIFSEFHKVNTVMVVFVVVCISIILLPILNKHLLVLLNEHAYLNKLL
                     GMPDDEQNKIVKRFMEMGHLTARECEIASLLIKGKTYRMISHELSISENTVKTHIKNI
                     YSKLNIKSRSDLMNVLISNEILTN"
     gene            complement(9827..10765)
                     /locus_tag="PLQ68_07550"
     CDS             complement(9827..10765)
                     /locus_tag="PLQ68_07550"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HXK72545.1"
                     /translation="MAVAAIFIAVYHLWIPVFSSASLAGRFENFFVSIGYIGVDLFFF
                     LSAYTLTLSDMTSKWKFIFKRFKKVYPVFLIFCVIALIIGKMSLSQFFSTAIFLDFFK
                     NGGGSFLWFIPALLLMYVAFPYCKSILSKYSPLKRLAISIIVWVTLSFAVEYGLSGIV
                     DISIFLMRIPVILLGIFLAEYEGLWSKKRRLILAATLFLPGIVLVYQFGYLDKLTIPF
                     DSIFYIVALPCILGLIFLLDVAFQGVSVKVVDILGNATLELYCIQMLIGSELVNIIFS
                     ITRVKILTNLITFITVILLSIALSYLRRYIINRKVA"
     gene            complement(10798..11361)
                     /locus_tag="PLQ68_07555"
     CDS             complement(10798..11361)
                     /locus_tag="PLQ68_07555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72546.1"
                     /translation="MKKLLPILLLLVLLTGCFNEGPDVTIPSETPAPSDTNVPSDLTM
                     AQIIDLICQDVEVPAYEAAELTDENFEYSAFIPYEEGLTGYKADALINATAHSLVLIH
                     SENGNTADLAQKILDNANPFKWICVSAEVTQTAYTEHYVILVMSSAEIVEGLIDNFQS
                     AVNDGDATILDSVSVDLLAINSEFIAD"
     gene            complement(11671..12453)
                     /gene="heR"
                     /locus_tag="PLQ68_07560"
     CDS             complement(11671..12453)
                     /gene="heR"
                     /locus_tag="PLQ68_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heliorhodopsin HeR"
                     /protein_id="HXK72547.1"
                     /translation="MENSVFVKLRRFNLFMGCLHFVQGLMMLILSLTWSNIKEFTPII
                     YSYFLKFDTQAMRLVTATDQLFKLPFGILVSAFLFLSALAHFIISVPKKTNDIYNRDL
                     KKNINQFRWYEYALSSSLMIVLIAVLFGVFDIGALIAIFLLNASMNLFGLLMEKMNQN
                     REKTDWTAFIFGSVAGLGPWIVIALYGFGNADPAEVPWFVYAIVGTYFLFFNLFPINM
                     ILQYRKKGKWADYLHGERGYIILSLCAKTVLAWLVLFGVMQP"
     gene            complement(12537..13058)
                     /locus_tag="PLQ68_07565"
     CDS             complement(12537..13058)
                     /locus_tag="PLQ68_07565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020492647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72548.1"
                     /translation="MKRKILTDYNVSYIADIVSIVQTQSKITLANWAIDYSEDVILRI
                     WQKYYPCDSRPNEAITAARRWLTGEVKLQYVKGKILDCHAAAREVAHNHVAFISARAI
                     AQCASTIHSARHCMGLVMYGSLAIAYDKLGYDASKAKIDEYIKDECIRMKDALVRVSV
                     DNEKNPIGKVISK"
     gene            complement(13071..13433)
                     /locus_tag="PLQ68_07570"
     CDS             complement(13071..13433)
                     /locus_tag="PLQ68_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016207692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HXK72549.1"
                     /translation="MKEAEKPYETVDEYIQLLPDDIKAYIIEVRNTIQKSLPNAKEKI
                     SWKMPTYWDKHNIIHFAAHKKHIGIYPGVDAIIYFAERLKKYKTSKGAIQFRYDEAIP
                     LDLISDIAKWCYETGNHH"
     gene            complement(13643..14158)
                     /locus_tag="PLQ68_07575"
     CDS             complement(13643..14158)
                     /locus_tag="PLQ68_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013467716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HXK72550.1"
                     /translation="MMPTHKMNTLVIVSSYHQMNTEKIAMRIARVLNANIVSAKKVNA
                     DKMSDFEMIGFGSGIYDAKHHINILSLVDKLPVRKNMKAFIFSTDGAPRFIFKSELWL
                     KDKMNKDHAVLREKLKDKGYEIVGEFNCPGLNKNSFLKYIGGFNKGRPNESDLLMAEK
                     FANKLQNDWLI"
     gene            complement(14142..14363)
                     /locus_tag="PLQ68_07580"
     CDS             complement(14142..14363)
                     /locus_tag="PLQ68_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006992993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HXK72551.1"
                     /translation="MKNHLPIKNNIRKLRFFANEMTQQQLAKNAGVSRQTIIAIEAGK
                     YSPSLELAFKIASIFNVEIGEVFEYDANT"
BASE COUNT         4832 a         2684 c         1981 g         4995 t
ORIGIN      
        1 atatgattac attttccgga acttgcgttt caattggaaa gaaatttgaa ctagacttca
       61 aacttttaaa cactgcatat gaaagctgga tgactttaga tgaaggggat aaagttaaaa
      121 catcatttga aataacagaa ggaagtgtcg atatatatgt cagacatgaa gataacagta
      181 taatctacca aggaaatgat gtggaaagcg gagattttat tattgagata agtaaatcag
      241 acaaatacta ttttgaagtt attggctctc atgctgcagg atatgtacat ttcaataaat
      301 taccttagta tttcattcaa tcaacaatat ctgttttatc taattgtccc atttcaggat
      361 tatatacagt aattctattt cttccatttt tcttagaaac atacaatgca gtatctgctt
      421 tatctatcaa aactttcaaa tcttcttttc cagaaaaaca ataaatacct gctgaaaaag
      481 taactcttcc tattgttgga aaatcatatt tctcagtttc ttttcgtaaa tattaaatcc
      541 gcaattgata tcaaaagatg caataagcag tttctgtatt tctttttaga taattatgaa
      601 atgaccattt attgctttcc cataatttcc caaacaatca aactcccagt aatagaaata
      661 ttttacccat ttaacaatcc tggtcagttc ttaagacttt tatcttaata caacctaact
      721 ttttgaagct gactgattac aatactttgt ataagagatt caaactgctc tgttttacca
      781 gatcttactt taaatatctt tacttccgag atagtatctt ttatttgtca cattaattat
      841 aatgttatat aaaaatacac catctgacgc cgtacttgtc aataagatcg gtcattaatg
      901 gactatactc acactctgat aacggataaa taatttgtgc atcatctttc aacacttcat
      961 atgctcttct gactttatcc tcattacctt gtccgtaatg taaacaaaac tgcatggtgt
     1021 taccagttat tgcttcttta ctgttctgtg agttttcatc atttcgttct gaaaccgcca
     1081 gaacctgtcc ttcaatatcc agttcggaat gcatatatgt accatcgtca taagaatatg
     1141 cgcatagaag cgttgcatca aaggcctttt gataaaagac aactgctttg tcactgctgt
     1201 taacatatac ctgcattagt gatcttttca taattggtct ccttactcaa ttgtatatgc
     1261 ttatattaca aattatttta ttctgtaata acaaaaaact tatactttga actatttagt
     1321 cattttttcg tttagctctt ttctccagat acatctccct atctgcatat ttaattaagt
     1381 catctgcatc ctgtccgtca cttggatagt gtgcaaaacc taaacttatt ccataatagg
     1441 gcttctccat taccatttca ttggaaacag attttttgaa atcttcacta tcaaaaccat
     1501 ctgatatatt tgtaatcaaa caaatgaact catctcctgc aaatcgataa acatcacctt
     1561 gcttgccaac agcgttttga agagctttag caaattgctt taaatatgta tcacctgttg
     1621 aatggtcata agtatcatta atcattttta ggttgtccaa atctatgaaa acaaccaaaa
     1681 attcttgttt gttattaata tattcattca gctgtttata caaagaatag cggttgttaa
     1741 gacccgttaa agaatcctta taagcaattg ttgaaattgc tgcagtttct ttttcaagtg
     1801 atgtaacttg tgttgatagt aattgtttat ctcgttcaac ctttgtttta gtctgaattt
     1861 ctagaaataa atagaaaatt atcgcatata taagcagtga cagaattgta cctatatatg
     1921 catgataaaa gtatactggt cgataaaata ccgcataggg aacaaacaaa atgaaatagg
     1981 aaagagcata tgacataata agtacgctat ttaacatcca ccatcctttt tttagcactt
     2041 cagtcatttt taaataagta ggacgtatta attttattac tactggtaaa aagataagat
     2101 aggtcaaaac gcgagcagca gtatctatca atggattatt ttctccataa aatatataag
     2161 aagctaaaag accatttatg atcgcaacag tcccaaaaag ctgtgttgtc agaataataa
     2221 agataaattt agctgcctta aaagcagaaa aatataatcc caataagata tatggtaatg
     2281 tgagagttag aaaatagaat cgaatataaa acgaaatacc aataaattgg attagtactg
     2341 catttatgcc tgtaataata atcgatccca aaatagcaaa tattaagctt ctgcgtttag
     2401 gtttctttaa aggcagcata attaatatat ataacacagt aaatgcttga gatacaaaaa
     2461 gtcttaatat tgtgccgtca tttaacatta ttataccccc tccaaaagta ataatgagtt
     2521 aacatatata aattaactat accataaata aactatttta gtatctgtga aaagatatta
     2581 gtttagctat ttaattatta atgcatgttg tattttttgc tgtcttttac atgaaaactg
     2641 taaaagaatc attcatttta taatttttcc gagtattgtt aggataccat tcaaaatcag
     2701 atatacagaa acaagaatag agccaatcaa atcaacgtaa taagttaaac tcccgcttgg
     2761 agctataaca attattacaa gagttactgt tacacataaa tccacaagca gtttagcaaa
     2821 atacagtttg ctttgtactg ataaaatggc atccggattc tccctgttaa gtttgctgta
     2881 tctaatccag aacaaaaaat taacgacaac tcctaataat gcaataatta aacctggaac
     2941 aacattacct ttttcagcat tcgaacgaaa taatgcaaca aaaatcattg caatacctga
     3001 aacacacatt gcaattccaa cagataaatt tgcagtatgt tcaagtttct ctttacgttt
     3061 gatatccgtt ccttcatttt tacttaaata acggaataca acccatgata caattattgc
     3121 agctaattcc gcagttcgtc ttataaagtc tacaaactga gtagaagaac gtcctaatag
     3181 cagtcctaaa cccaccacaa tgggacctgg agaacttaga ataacagaag ctagaagtgt
     3241 acgttcacca gatttctttt tcataaataa atcacgcttc cctgcaaata cagcaaaaga
     3301 atcattgtaa aagcagctgc caaggggcat gtcaaagtat ccattccacc ttttgtatat
     3361 agttcaacaa cggcactaac agctgctgta attatggcaa tcagcaaata gctataccaa
     3421 tttaatgaac tataagctat gagaattata aaaactgaaa tgaaagaaac gacaaacatt
     3481 gcaaatgtac cctcaaggct cttctttcct tcaaccagtt tgccttctat gtagtattgt
     3541 ccaaaacgtt ttccgactaa agccgcagct gcatcaccaa agccccaggc aaagactgat
     3601 gcaataacca gatatttcaa atttagcaat ccccagcata cgctaatcaa tattgaaaac
     3661 attagaaata ctataagaag gctctttttg atttcaccct tctttcgttc tgtaagaaga
     3721 ttagaatatc caggtattct ttctgcaaat aacaaaattg gaaatacaat tagtgcaaat
     3781 aacactgatg ctgctacaga aatccaccat gtactgaatg catatgttaa cacgaatata
     3841 gaacatatta atatcatatg aagtaattta cgaaatactt catttggcat tttaacaaat
     3901 cgcttacaaa ttagtgcaat tgttgcacaa actataaaat atcctaaaag aataccaaaa
     3961 cctataaaaa attcagtcat tgcagtctcc acaatgttga aggttttttg agatacgtga
     4021 taaaagtgtc tctatattaa taaaatcatc atctgcaata ccttttcttg caaggctata
     4081 taaattatca taaacttctg taatgctttt actgattttt aatgctttat cagtgagaat
     4141 aatggtatat gctcttctat ctgtattact tcttgtacga gaaacataac ctttaaatac
     4201 aagagaatca actgcacgtg ataccacagc ttttcctata tccagctgct ctgcaagttg
     4261 ctcctgttgc agattgtcac ttcctgttac aagcaaaaac aataaatcac cttcggttcc
     4321 tgttaaacca aatggttcca gttgagcatt tataatcttt cttgcagctc gtattacgcc
     4381 tttcatatca agccacattt ttcgtatcaa aagcatttaa atttctccat cgagagtgat
     4441 tatatccatt ttttagttga ctagtcaact aaaaggtatt acaagtgaga gcaaattaaa
     4501 atgtgaattg agaaattaaa attgtgaatt aaaattaaaa taaagagaaa acatatatcc
     4561 atttacgttt tctcttttta tgaagatatc cattattaat taatacggtc tgttagtttg
     4621 attctttgaa tttttcttat ttaccgatat tactaccaca atacttgcta aaacaacaac
     4681 tgctcctgca ataaacaaaa ttgttgtaat atcaatgcca gttttttttg atcccttcat
     4741 agccgaaaca gttatctcat ttacattatt atcccatata tcaagttcag cattaacatc
     4801 aaacttctgt atatcactag attttccttc gattgcgctt aacaaattac cagctgatat
     4861 tatagaagtt gaattatcac tgtattgaag catctccact tctccctcaa acactttcac
     4921 tgtcgaagtc tgcatatctt cctcaagtac aaaggttgta ccttttatag taacagttgc
     4981 ttgtgacatt ttaatgctaa tatgcgaatt acttgctatc tgtctaatat tgaaccatgc
     5041 tttcccatta attaaagtaa gtaaatttac ggtatgagat tggcctgaca gggtaatcat
     5101 tgaatttgaa gatagaataa gcgtattcat attattcatc agtacgacag cctcactgtc
     5161 ataatctgtc tgtacaatag cattcagagg taactgttca agaccaggtt tccatgaata
     5221 ccaataccca tcctttcctg acaaaatata ttcctcatca tgctcttgcc aataatatgg
     5281 atttgatctt tctacccaga tccaaacggt aacattacct gaataatctg ctatttctgc
     5341 tcctgtaaaa tcacctgcgg gttttggatt tacaggtaaa acgccatttt taggagtttg
     5401 attatacatt ccatcacgat tcactactgg ttgcgtaggt tgtatctgat tacttaattt
     5461 ctgccattgg taaacataat tagttcccat agaaattccc cgataaaaat gatatcttaa
     5521 tgctataagc tctccctctt ccttacctga accgagtttg ccacttactg tagcagttac
     5581 atccatataa ccttcttgaa gagtaatctc aaagtggtgc tgaccatcct tgtctttaaa
     5641 aagtattgct ccttgggttc ttgaacctgg tccgagatca tatctgtcaa agtctactgt
     5701 cgcacttgca ctaaaaccaa aatatgataa tgaattttct gatgcagtca tattaatagt
     5761 aatggttact tcctcaccag gtattatttt ttctggaact ccagtaaaat cagcactaag
     5821 ccttatagct tcaccatata tctttggtgg atttctccag tcctcagtcc ttcctgtata
     5881 aatagtcttg acagaatatt gacctgtaga acatgagtat tcattattat atgattcatt
     5941 ttcattagca actgcccact tatcctcatt atcataatta acactattta taagaatcca
     6001 ggcatactga tcttcctgtg aatttactga aatatacgga atcattaata tcattaccaa
     6061 taatattgtt ataaacttta cgatttttgt attcataatt atcctcctct aattcattaa
     6121 cggacattta cagcggtaac ataaatcatc agacatatta tttttgcttg agcaggcaat
     6181 acaatatact attgtttcat tagatttatc atatttctct aaaatatgag atgcaggcat
     6241 ttttccatga tgtcttactc tctctccaat tttgtagtaa tcaaatagct gctctgtata
     6301 taccatgctt tcaacttttc catcatccct ttgaatatca atgtaatact gattctgtag
     6361 atgaccctgc tttctttctg cataagtagg tttttttatt cttttatcta taatagttcc
     6421 atcccaagtg atatcttttt tgcttttcct aagctgaaaa agtgctaata ttactaacag
     6481 gcccgcaaat gcaagtccta tgtataaaga tgatggaaat tcgaaacctc caacttctgc
     6541 caatcccagt attgtaaaca ctattagtgc aataacagag agtatcagag tgaaaactgt
     6601 tcccgaactt ttcatctttt ttactatctg atgaactctg ggatcgttta tcctgtctga
     6661 aaaccctatt ctgtttccgc tagaatagcc tggttctgat ccgctaccta tattcaactc
     6721 tgcaccgcaa ttaacacaga acctcatatc attttctatt tttgtattac agttttgaca
     6781 atacattaca tcctcctctg tttatattat tcgtctttca caactatatt gaataccagc
     6841 gataacatat aatcataatc tatatctata gtatcagtct gactattatc atcatatgtg
     6901 ctttttccat ttgcttttaa aaggtccacg ctggtaatcc ttattctttg gccatctctt
     6961 tcactgaaaa tctgcatatc accttctacg gttacatcat aatcttctac taaaggtctt
     7021 ggccaatcct tctggtgaat tacataattc aaattcacat ttaaggttcc ctgtccaatt
     7081 aacgtacctg ttgttactcc cgcaagttgt tgtatgtcct gtatagtacc aaagatattt
     7141 agttctttta catttacttt agctacttca ttttcaaaaa gtaaacctga aaactctata
     7201 gaaaaattgc cattttcaga aacaggtatc tctcctagct ccttaatcat tgttgaaaat
     7261 gccaaatgcg gagtataaac attatcccag cttactgtat cccatgaatt ttttgttatg
     7321 tatatataat aattctgatg cttaacttgc tcttcacctt cttgttgatg tgttgattgt
     7381 ggtgtttgtg aagttaaact agagcttagt gtttttactg gtttttcagt acagccagca
     7441 agaacagtta gtgcaattat gattgttaaa gctatgataa aatatttttt catttcaatc
     7501 ctcctggtat aatatttttg gaacaattcc tgtttcaaaa tgaaatttct ttgaaatatc
     7561 atttatttga ttttggtcaa tttttttata taaaagcctg atttgatttt cttttttgtt
     7621 tgagccatat gttatgctta tatagtcatg atacaactcg acagttttaa gatgcacttt
     7681 accttgatta aatctaataa cttttgtatt aaggagtatg ccaaaacatg atatagtcat
     7741 atctatactc tgctcatctg actgtccatc ctgagtcttt attaatacag gctgaatact
     7801 cataatcttg ttttcaataa ttaacttagc ttgctttagg ttttgaaatt gctttattgt
     7861 taaaacaaga gatattactg ccagaaaact taaagtcatc agtattatcg tcttaaattg
     7921 cgaactgctt tcaaatttag taattatgaa aaacagaaag aatataatag gtgccaatat
     7981 actgatacat ataaacagtc tgacttttat ccgtttcaca tcataagctc ccttcatcaa
     8041 tgttttctta tgtactgctt ttaattttat atatactgaa tataaataac tatcacccaa
     8101 aagggtgatt tttatgcaaa tcaattaata ttgaactagt tagatatcaa tgttaattag
     8161 tcagtatttc atttgatatc agaacattca tcagatctga tcggcttttt atattcaact
     8221 tcgaataaat atttttgata tgtgtcttaa ctgtattctc acttatactc agttcatgtg
     8281 agatcatcct gtaagtttta ccttttataa gcaatgatgc gatttcgcat tctcttgcag
     8341 taagatgtcc catttccatg aatcttttaa ctattttatt ctgttcatca tcaggcatac
     8401 ctaaaagctt attcagatat gcatgttcat ttaaaagtac taatagatgc ttatttaaaa
     8461 tcggtagcaa gataattgat atacaaacta ctacaaatac taccattact gtgttcacct
     8521 tatgaaattc agaaaatatc attattcgac ctacaacccc accgaccaac aatccaagta
     8581 catttgaaga taaccctatt cctaaaattc ttgaaggatt tctatgccag tcaagcatct
     8641 ctcctaatat gctccaccaa aataaatcat atataccaca tgctcccagc atgagagtat
     8701 taacaatgaa ataactgatg acattatgtt caaatatcat aaaggctatg tatgataacc
     8761 caatcatggc aattgcaaca taaagaatgt atgtcctatt aaatttttta ggaagatttc
     8821 tcattactaa aagcgctatt atgtatggta ttgcccaata ccagctcgtt aaacctctta
     8881 aatgtgaata tgcaggtcct tgaacctgat acataagacc tgagcttata gtcaatataa
     8941 ctataaaaag acacagaaga gctaaaggtt tgattacatt agactgtcca ggtatcgaaa
     9001 tatcctcatt gtcctttttc tcatcatcct tttttggaag cattgatgca aaaagcaaag
     9061 ctaatattaa cactcctatt gaaaatatta accctgtttg aactgaaaca tgcacagctg
     9121 tcatgtttat tagtatcatt aatacatttg aacctattaa gctatcagca ataatcttta
     9181 ttctttcact tctatgagta gaatttttta gaaaaaaagc ccaacctgca acacagcttc
     9241 cagttagaaa ggaaccagat aagacagcta ttatccataa aaccgacgga gtcataaaaa
     9301 atactgtagt tgcaatcata cagtaaatta ttgagataag cattagcaat cttgctctgc
     9361 tttttgtttt tattattaga ctactaacta taagcccaat aaaatggaaa aatattgacc
     9421 caaatattaa aaaggttcca tatgaaattt tcgtaatttc aattaaggta tagagtattt
     9481 caccttcaaa aggtatggaa agcagccaag cgaaaaatag tgagaaaaca agaatggtta
     9541 atcttctttc gtttattaca ggcttgatta tcttcatcca gtcacttact ccctctctac
     9601 tgattaatta ataagtacca cagcttatgc atatttttta tacaaaaaag cataattcgt
     9661 tttttgagtg ctaattatac tatatttttt atacatatgt aaaatctgac taatggtgaa
     9721 caaaagaaag ataaatattt ctcagtaaat ttcaacaaaa atggtataag tttatgtaat
     9781 aaacaagaat aaatatggaa atataacttt caacactaaa ttcacttcat gctactttcc
     9841 tattaataat atatcttcgt aaataagata gggctataga taacaatatt acagttataa
     9901 atgttattaa attcgtcagt atcttgactc tcgtaatact aaagattata ttaacaagtt
     9961 cgctacctat aagcatttga atacaataca actctaaggt tgcattacct aatatgtcaa
    10021 caacctttac tgaaacgccc tgaaacgcaa catcaagcaa gaaaatcaaa cctagtatac
    10081 aaggcagtgc tactatgtag aatatactgt caaatgggat tgttagttta tctaaatacc
    10141 caaattggta tactagcaca atacctggta aaaataatgt cgctgctaat ataagacgtc
    10201 gttttttgga ccataatcct tcatattctg caagaaatat tcctagaaga ataacaggaa
    10261 tgcgcattag aaaaatacta atatcaacta ttccacttaa accatattca actgcgaagg
    10321 ataaagttac ccacactata atagatatgg ccaatctttt taaaggactg tattttgata
    10381 aaatggattt gcaatatgga aatgctacgt acattaagag cagtgcggga ataaaccaaa
    10441 gaaacgaccc gccaccattt ttaaaaaaat ccaaaaaaat agcagttgag aaaaattgag
    10501 ataaagacat ttttccaata attagagcga taacgcaaaa tattaaaaac acaggataaa
    10561 cttttttgaa tcgtttaaaa ataaacttcc atttagaagt catatcggac agtgtcagtg
    10621 tataggctga cagaaaaaag aacagatcaa caccaatata tccaatacta acaaaaaagt
    10681 tctcaaatcg tccggccaga ctagcagatg agaaaactgg tatccatagg tggtacactg
    10741 caataaaaat tgcagcgacc gccatgattt gcttttttgt ttttagtatt tgcataacta
    10801 atcagcgata aactcgctat taatagctaa taaatcgact gaaacgctat ctagaatcgt
    10861 tgcatctcca tcatttacag cactttgaaa gttatcaatc aacccttcaa ctatctctgc
    10921 tgaagacata actagtatta cataatgctc tgtatatgca gtctgagtta cctctgcaga
    10981 aacacaaatc cacttaaaag gattagcatt atccaggatc ttctgtgcaa gatcagcagt
    11041 atttccgttc tcgctgtgta ttaacactaa agagtgagca gttgcattaa ttaaagcatc
    11101 agctttgtat ccagtcagac cttcttcata tggaataaaa gcagaatact caaaattctc
    11161 atcggttagc tctgccgctt catatgctgg aacttctaca tcctggcata tcaaatcaat
    11221 aatctgtgcc attgttaaat ctgatggcac attggtatct gatggtgcag gagtttctga
    11281 tggtattgta acatcaggtc cttcattgaa gcaccctgtt agcaaaacaa gtaatagtag
    11341 tataggtagt aatttcttca tttttttgtt tcctttctta agccaaactc tttcttagac
    11401 cgtcatattg agataaagtt ccaataaata tattcttaat agaaaacttt tgtatactaa
    11461 ttatcaagta atctttctac tataatttaa ttatgatgat taaatcaact ttttacgcaa
    11521 attaaaaatg cagttaatag caactgcatt tgaaatgaat aagaatatac ttctaaggct
    11581 atctatactc ccataagcaa tacaacaaag agtaagtaat cttatcagta aaaattatta
    11641 ccaaaactat ttaacttcct gtaattaatc ttatggctgc attactccaa acagcacaag
    11701 ccatgcaagc actgtcttag cacataagct cagtataata tatcctctct cgccgtgaag
    11761 ataatcagcc catttgcctt ttttcctgta ttgtaaaatc atattaatag gaaacaggtt
    11821 gaagaacaag aaatatgttc caactatagc atacacaaac catggtactt cagcagggtc
    11881 agcatttcca aatccgtaaa gagctattac tatccacggc cctaatcccg ctacagatcc
    11941 gaatataaat gctgtccagt cggtcttttc cctattctga ttcatcttct ccattaaaag
    12001 accaaaaagg ttcattgaag cattaagaag gaatatcgct atcaatgctc caatatcaaa
    12061 tactccaaaa agtacagcaa tcagtacaat cataagtgag gatgataaag catactcata
    12121 ccatctgaac tggttgatat ttttcttaag atctctgtta taaatgtcat ttgttttctt
    12181 tggcacgctt attataaaat gcgctagtgc cgaaagaaaa agaaatgcag acactaaaat
    12241 tccaaatggc agtttgaaaa gttgatctgt agctgttaca agcctcattg cctgagtgtc
    12301 aaatttcaaa aaatacgaat agatgattgg tgtaaattct ttaatatttg accaggtcag
    12361 agacagtatc aacatcatta atccttgcac aaaatgcaaa catcccataa aaagattgaa
    12421 tcttctaagc tttacaaaaa ctgaattttc catattttat tacccccgtt gatgtaatta
    12481 tatcttattt aaaaaacaaa taaacaagat tgtgtattaa taaattattc ttgccattat
    12541 ttagatataa ctttccctat aggatttttc tcattatcaa cagaaactct aacaagagca
    12601 tctttcattc tgatgcattc atcttttata tattcgtcaa tctttgcttt tgaggcatca
    12661 tatcctagtt tatcatatgc tatcgccaaa gaaccataca ttactaaacc catacagtgt
    12721 ctggcagaat ggattgttga tgcgcattgt gctatagctc ttgctgaaat aaatgccacg
    12781 tgattgtgtg ccacttcacg tgctgctgca tgacaatcaa gtatttttcc cttaacatat
    12841 tgcagtttta cttcacctgt caaccacctt ctggctgcag ttattgcctc atttggccta
    12901 gaatcacaag gatagtactt ctgccatatc ctcaaaatta catcttctga gtaatcaata
    12961 gcccagttag caagagtaat cttgctttgt gtttgaacaa tgctcacaat gtcagcaata
    13021 tatgaaacat tgtagtctgt cagaattttt cttttcattc acgataacct ctagtgatga
    13081 tttccagttt cataacacca cttagcaata tcagatatca gatcaagcgg tatagcttca
    13141 tcatatcgaa actgaattgc tcctttgctg gttttgtatt tttttaatct ctcagcaaaa
    13201 tatattattg catcaactcc tggataaatc ccaatatgtt ttttgtgtgc agcaaaatga
    13261 attatgttat gtttatccca gtatgtaggc attttccatg atattttttc tttagcattg
    13321 ggtaatgatt tttgtattgt gtttcttact tcaattatat atgctttaat atcgtctggc
    13381 agtaactgta tatactcatc tactgtctca taaggttttt ctgcttcttt cataatctat
    13441 tctctctttc aataataacc gccttaatac tatctttgga ttgttttttg cttttttact
    13501 ggcatctttt ctaattccga ttatatttta aactgtttcc attttaacaa tctgcccatc
    13561 ttatgaataa ccgtcaacat cagaaacttt taagataaac ttcagaatat gcaataatat
    13621 tagtaacatg cttcctgtat gtttatatca accagtcatt ttgaagttta ttagcaaatt
    13681 tctctgccat tagcaaatca gattcatttg gtcttccttt attgaaccct ccgatatact
    13741 ttagaaaact atttttgttt aatcccgggc aattaaactc ccctacaatc tcataacctt
    13801 tgtccttaag tttttctctc aacacagcat gatccttatt cattttatct tttaaccaca
    13861 attcactttt aaatatgaaa cgaggagcac catcagtgga aaatataaag gctttcatat
    13921 tctttctaac cggaagctta tcaacaagac ttaatatatt tatatgatgt tttgcatcat
    13981 aaatccctga cccaaatcca atcatttcaa aatctgacat tttatcagca tttacttttt
    14041 ttgctgaaac tatatttgca tttaatactc ttgctatcct catagcgatt ttttccgtat
    14101 tcatctgatg gtatgaactg actataacaa gtgtattcat tttatgtgtt ggcatcatat
    14161 tcgaaaacct cccctatctc aacattgaaa atactggcta ttttgaaggc gagctctaat
    14221 gatggtgaat acttacccgc ttcaatggct attatggtct gtctggaaac acctgcgttt
    14281 tttgctagtt gctgctgagt catttcgtta gcaaaaaaac gtaattttcg tatattattt
    14341 ttaattggca agtggttttt cattatttaa tatccaaatt taaaagattt tcattagtgc
    14401 actaatagga ttttaaatac ctattttaat gtaaagtatt atagacatat agtatatata
    14461 tatttacctc ttgtcaagta tatttaacat tt
//
LOCUS       DAOSXM010000055        14470 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2111
            NODE_2645_length_14470_cov_10.541034, whole genome shotgun
            sequence.
ACCESSION   DAOSXM010000055 DAOSXM010000000
VERSION     DAOSXM010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864178
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 14470)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14470)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:24:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,261
            CDSs (total)                      :: 2,229
            Genes (coding)                    :: 2,218
            CDSs (with protein)               :: 2,218
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 3 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14470
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2645_length_14470_cov_10.541034"
                     /isolate="AMR_MDS_2111"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            134..1240
                     /locus_tag="PLQ68_07585"
     CDS             134..1240
                     /locus_tag="PLQ68_07585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015712084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HXK72552.1"
                     /translation="MEPSVQKIHDFYNLKKDAPIFQKEFGWYVKDKWKAQGWINDETD
                     LWELFQFDNSAVHYLGKCGWCEAPLEPLFEKKVLETNNECEIVQDTAGRKVLYFTGRR
                     DGFMPSYLDHPVKDMKTLEENILWRMNPNDPIRYIDIEERMNKAKEFENRNFIICANV
                     SGGYMYLRSLIGPEDLLYKFYDDPLLVHRCMEIWFDVMDKVCETHQKYVSIDELFFGE
                     DICYNHGPLISPDMIKEFLFPYYQQLIENIKKRQLNKNKKLNIQLDTDGYSVAVIDLY
                     KTIGMNYMSPFEVASGCDVVEVRKKYPDLLISGGFDKRILAAGKDAIDKEIKRIMPFM
                     KKHGGYIPTCDHGVPEEVEFTDYMHYRKRMLEYK"
     gene            1304..3361
                     /locus_tag="PLQ68_07590"
     CDS             1304..3361
                     /locus_tag="PLQ68_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011025214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III"
                     /protein_id="HXK72553.1"
                     /translation="MEGILQIFASNVYNDKVMKEELPAYVYESLKKTIEQGGPLDDGI
                     ADTVAESMKDWAIRKGATHYTHWFQPMTGITAEKHDSFLMIRNGKPVMELRSKELIKG
                     EPDASSFPSGGLRATFEARGYTTWDATSYAFIKDRTLYIPTAFCSYCGEPLDNKTPLL
                     RSMDALNKQALRIVRLFGNTTASRVIPVAGCEQEYFLIDKKQYDKRKDLLICGRTLFG
                     AKSPKGQELNDHYFGNIRERVQNFMRELDIELWKLGITAKTEHNEAAPAQHELAPIYA
                     TVNIAADHNQIIMEMMKKVAEHHGLACLLHEKPFANINGSGKHNNWSISTNEGDMLLS
                     PGKDPSKNKRFLLILAAVLKAVDEYSGLLRQSIATAGNDHRLGSHEAPPGIISVYLGA
                     ELTEILEDLENGRTAHNKAREKFCMSETLPVLFKDTCDRNRTSPFAFTGKKFEFRMPG
                     SSCSVSDANTVINTIVANSFKEFADILEKSDNFDKTVDDLIKQTVLKHKRIIFNGNNY
                     SSQWLEEAKRRGLPVLNATPEAVDCLVEDKSFKLFETFGIFSKTDLISRHEIKMENYC
                     KIISIEASTMIEIAKREILPSSMDYASTIYSSILMKKDIGLDISTETLIAQNLSDNIK
                     NLNDCIIELEKVSANTENAYDFKDKVIPVMNKLRKYADSLECIVSKDYWPMPTYSDML
                     YSV"
     assembly_gap    3451..3550
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3551..4340)
                     /locus_tag="PLQ68_07595"
     CDS             complement(<3551..4340)
                     /locus_tag="PLQ68_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004221041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HXK72554.1"
                     /translation="MSSIQIPKDYQSKLSIYETQLAIGSLKRIFEENLCRALNLKRVS
                     APLFVDSQSGLNDNLNGVERPVCFETKENDMQAEIVHSLAKWKRLALHRYNFPAGEGI
                     YTDMNAIRRDEDMDNIHSIYVDQWDWERVITKQERTLPFLKQIVCDIVSAICDTHEMI
                     NDIFPALEKTVTRNVSFITSQELEDMYPDLSPKQREDVYVKEHKTVFIMQIGKTLKSG
                     KKHDGRAPDYDDWEMNGDILMYNKVLDSALEISSMGIRVDSEALD"
     gene            4462..6810
                     /locus_tag="PLQ68_07600"
     CDS             4462..6810
                     /locus_tag="PLQ68_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004620253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HXK72555.1"
                     /translation="MAQKKRTSVSRKAAENSNKQVHSNVLTKEIFGIVIIALGLLAFV
                     CVFFTSKSGSFGRPFKQVTAGLFGWAHYLIPLAILLFGCYIALAGRVKVLSRKTVYLI
                     LLILIVPLFFQIVYLKNNMSDISNNMNVWQFIAHMYIIGRDNIHNGGVFGGIIMTPLV
                     IFFGTTGGYIITITATVVLVIYITNKSFLDFLIKVKNMFKAIFSWMKDAFKPIDDDSE
                     KKTVTTISKQPYIQDTIDDLPQVNKEPINIVMNRKSKDMPEQTPKKPVSRFTGPIIIK
                     KEEEKKSTMPVPKPENDQKKGDYVFPPLSLLDSYMDSGDSDNYEQIARKLENTLLSFG
                     VEVSVTNIMCGPTVTRYELQPKSGVKVSKIVALADDISLNLASRDVRIEAPVPGKSVV
                     GIEVPNTKKSVVSLKEVIGSKEFQGSKSTLTIAIGKDIAGNCTVADVRRMPHMLIAGA
                     TGSGKSVCINCIITSILYKASPDDIKFIMIDPKKVELINYNGIPHLLIPVITNLKKAA
                     GALNWAVEEMSNRYEMFNKRGVRDINSYNDSIKNDPEIKKMPNIVIIIDELADLMMTN
                     PGEVEESICRLAQLARAAGIHLIIATQRPSVNVITGVIKANIPSRLSFAVVSQVDSRT
                     ILDMGGAEKLLGNGDMLFHPMGLSKPVRMQGAYISDSEINKVVKFIKERSSSEYDDDV
                     VDNIEKSNNKTASTDIENEIDELLPAAIDLVVEIGQASVSYLQRKLKLGYGRAARIVD
                     QMELRGIVGPFEGSKPRAVLISKDEWAEMQMSNPELDVEKSR"
     gene            6807..8606
                     /locus_tag="PLQ68_07605"
     CDS             6807..8606
                     /locus_tag="PLQ68_07605"
                     /inference="COORDINATES: protein motif:HMM:TIGR03904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HXK72556.1"
                     /translation="MSFLPISREDMEQRNWEYIDILFVTTEAYVDHPSFGQSIITRLL
                     EDKGYRVAILSEPDISSCEDFKRFGEPRLATLISGGVIDSMVNNYTANRNRRRTDCYA
                     SKGKTRPDRAVIVYSQRAREAYKDVPIIIGGAEASMRRFAHYDYWQNVVRRSIIFDAK
                     ADLLVYGMGETAIVEIMKLLDKNVPISSITSVKGTAYISKDIPKAHKVFKDIVLPTFE
                     DISTDKIEYVKAFKIQYEQQDPIWGARLIQGHNDRFLVCNPMADPLSEEMMDYIYSLP
                     YERSYHPIYEESGGIDSLKEVKYSVTSHRGCFGNCSFCSIVLSQGRIIQRRSRKSILD
                     EIEKLTHENDFHGYIHDLSGPTANFVNPSCKKQLDKGMCKNKQCLFPTPCDQLEVSHE
                     YLLETLKEARKVPGVKKVFMRSGLRYDYLMLDRDKSVLEKICKYNVSGQLKVAPEHVS
                     DSVLELMGKPPFSVYYEFKECFEKANDKAGLKQYLLPYFISGHPGATIKETIKLTEYL
                     IESKFIPEQVQDFYPTPGSLSTCMYYTKINPLTMEKVYVPNAHDRRVERALLQFNIPV
                     NNKIAKETLISHGRKDLADKITIRIRQKKKAKK"
     gene            8603..9907
                     /gene="mtaB"
                     /locus_tag="PLQ68_07610"
     CDS             8603..9907
                     /gene="mtaB"
                     /locus_tag="PLQ68_07610"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HXK72557.1"
                     /translation="MKTYSIITLGCKVNQYESSSIAASFDAMGFEYKKFPDICDIYVV
                     NTCTVTNMSDRKSRQMMHKARSLNPDCVIIATGCLVQNKPEDMKANGADIILGNNEKP
                     YIEDIVNNYLNNKEDSLISVSDISRYKPYTNTRFADRWERTRGYIKIQDGCDNFCSYC
                     IIPYVRGRVRSRELTDIIKEAEFMASNGIKEVVLTGIHLNSYGKDHNEISLLDVISAL
                     NDVKGIRRIRLGSLEPNVVDEEFINEILKYPKLCPHFHMSLQSGSDNVLKDMNRRYDS
                     RIYLKAVKLLRDNLPDVELTTDIIVGYPTETEEDFLMSINLVTKAEFLKTHVFKFSKR
                     KGTKAEKLKPLNSMILNERSDRLISVADKTAYLKMSSYIGKTLEILVEEQKQGYVIGH
                     SRNYIKVQAHLKKETNTSYINNFYKVLITKADKNNLYGNLVE"
     gene            10027..10278
                     /locus_tag="PLQ68_07615"
     CDS             10027..10278
                     /locus_tag="PLQ68_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017414598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HXK72558.1"
                     /translation="MDKGSTINLDFLKSQQSETREILTLVFSAMTEKGYDPVNQLVAY
                     LMSGDPTYITSHNGARNAIRKLERDEIMAELIQYYFTSK"
     gene            10284..10703
                     /gene="ruvX"
                     /locus_tag="PLQ68_07620"
     CDS             10284..10703
                     /gene="ruvX"
                     /locus_tag="PLQ68_07620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007221604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HXK72559.1"
                     /translation="MIIIGIDYGEKRIGVAKSDPLGLLAHGITTIEWDRDIEKPINQI
                     QQIVIQHNVEKIVLGLPLNMDDTEGEKAIKVRDFADVLKKRINIPVEFYDERLSTVEA
                     HDIIHMQGKKTGKNKKRIDMVAACVILQSYLDNIKKK"
     gene            10715..10990
                     /locus_tag="PLQ68_07625"
     CDS             10715..10990
                     /locus_tag="PLQ68_07625"
                     /inference="COORDINATES: protein motif:HMM:NF018633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HXK72560.1"
                     /translation="MDNERDDIIMMEDENGNPVEMIALEMLDYEEKTYVLMQTTDETD
                     ENSYIFEYEEEDDDAILTPVEDDNLVDKLFELFIKKMDMDSEEEEEE"
     gene            10998..12755
                     /locus_tag="PLQ68_07630"
     CDS             10998..12755
                     /locus_tag="PLQ68_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011025286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HXK72561.1"
                     /translation="MFDLMNVEKPARYTGGEFNEIVKDHEKMLSSYAFCFPDTYEVGM
                     SHLGMRILYHVINNRDKTVCERVFAPWIDMEEQMRSHKIPLFSLETKKPVSEFDIVGF
                     TLQYEMSYTNVLNMLDLSNIPIRREDRNETHPIIMAGGPCSVNPETLYDIMDIFIIGE
                     AEEVINEVLDLFELMKKDNSSRNDFLRKVNDIEGVYVPLFHNLKKKIKRRIIKDLDKV
                     SFPTDSIVPYINIIHDRASLELFRGCIRGCRFCQAGFIYRPVREKKAQTLIDQTKDIL
                     DKTGYDEIGMVSLSTSDYSDLKTLCDGMLEHTEKRMVNLSVPSMRIDNFSIDLMKRIQ
                     KVRKSGLTFAPEAGSQRLRDVINKNLTEEEILNSVKIARDGGYDNIKLYFMLGLPTET
                     DEDVLAIAELGKKILEIFGRKRRINITISTSFFIPKPFTPFQWCAQITKQEMVRRQRL
                     LRDSIKEKKIKYNWHDFNTSIVESAISRGDRLTGKAIIRAWEKGCKFDSWNEFFDMDK
                     WVSAFSECGLDIYQQASREINSNDILPWDHIDIGVTKKFLKNEYKKAFDEITTKNCRE
                     ECTGCGIAKYKCGVCFENN"
     gene            12742..13368
                     /locus_tag="PLQ68_07635"
     CDS             12742..13368
                     /locus_tag="PLQ68_07635"
                     /inference="COORDINATES: protein motif:HMM:TIGR03936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03936 family radical SAM-associated protein"
                     /protein_id="HXK72562.1"
                     /translation="MKIIRFKFKKNEKVMYISHIEMMNAFTRALRRADFDVVFTQGFN
                     PGMKLVFALPLPVGVSSDSEYVDIWFNQDYKCQYVIDKLAQALPTGFELLEANSVEKS
                     TLMEDVGSAIYGYIVECDKNLKEALDKLISLDKIEVEKSNKKGPVDILPYIYKIDYKD
                     ETSFSVLCKAGAKGNLHPRLLLKALEKYTDCRIYEKSINRNKLFLKVQ"
     gene            13423..14157
                     /locus_tag="PLQ68_07640"
     CDS             13423..14157
                     /locus_tag="PLQ68_07640"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016430 - tRNA
                     (adenine-N6-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HXK72563.1"
                     /translation="MVKDYERLDDLQLDGLKLIQNRKHFCFGTDAVLVCSFANVKNTS
                     SVIDLGTGNGIIPLLLYGKFKPRLIMGIEIQKEAYELAVRNVKLNNLEDRISIINGDI
                     KNIKEYLQCGSFDHVITNPPYLKSGTGIISAEDSIAYARHEILCTLDDVLKAARYLLK
                     SKGTFTMVHRPDRLNEILMQMKNNGIEPKTIRFVHPNEKSQAILVLIQGLKDMSPSLV
                     VDHPLIIHKPDGSYTEELNMLYGRES"
     gene            14157..>14470
                     /locus_tag="PLQ68_07645"
     CDS             14157..>14470
                     /locus_tag="PLQ68_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011175807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HXK72564.1"
                     /translation="MLYLVATPIGNLSDISKRAIEALENCDYIASEDTRHTLRLLNAF
                     GIKKKLVSYHQHNMEKSGQSILNDVMSGKDVCLVSDAGCPAISDPGNELVKLMHKNNQ
                     KVS"
BASE COUNT         5256 a         2031 c         2765 g         4318 t
ORIGIN      
        1 ctgaatatat atatattatt tttacgaatt tatattattt attgaatttt tatatgtata
       61 gtgttttaaa aagtacttga ttgaatgctg ttgtgttata atctcttcta taaatacttt
      121 ttaggagggc tatatggaac cttcagtcca gaaaattcat gatttttata atttaaaaaa
      181 agatgctcca atatttcaaa aagaatttgg atggtatgtt aaagataaat ggaaagctca
      241 aggttggatt aatgatgaaa ctgatttatg ggagcttttt caatttgata attcagctgt
      301 tcattattta ggtaaatgtg gttggtgtga agctccgcta gagcctttgt ttgaaaagaa
      361 agtactagag acgaacaacg aatgcgaaat tgttcaagat actgcaggaa gaaaagtttt
      421 atactttaca ggcagaagag acggatttat gccctcatac cttgaccatc cggttaaaga
      481 tatgaaaaca cttgaagaaa acattttatg gcgtatgaac cccaatgatc ctataagata
      541 tatagatatt gaagaaagaa tgaataaagc caaggaattt gaaaatcgta atttcataat
      601 ttgtgcaaat gtatctggtg gatatatgta cttaaggtcg cttatcggac ctgaagatct
      661 tttgtataaa ttttatgacg acccattgct tgttcacaga tgtatggaaa tatggtttga
      721 tgtaatggat aaagtttgtg aaactcatca gaagtatgta agtattgacg aattgttttt
      781 tggagaagat atttgttata atcatggacc tttgatatca cctgacatga taaaagaatt
      841 cttattccca tattatcagc aacttattga aaatattaaa aagaggcaat tgaacaagaa
      901 caaaaagctt aatatacaat tggataccga tgggtattct gttgcagtaa ttgatcttta
      961 taaaacaatt ggaatgaatt atatgtctcc atttgaagta gcatcaggtt gtgatgtggt
     1021 agaggtaaga aaaaaatacc cagatcttct tatctcagga ggttttgata agagaatact
     1081 tgcagcaggc aaagatgcaa tagacaaaga aattaagagg ataatgcctt ttatgaaaaa
     1141 acatggagga tatatcccaa cttgtgatca cggagttcct gaagaagttg aatttacaga
     1201 ttatatgcac tatagaaaaa gaatgctgga gtataaataa acaaaatatt gtaaaaatat
     1261 gatttgtttt atataatgta aaaaattaac cggaggaaac ccattggaag gcatattaca
     1321 gatttttgca agcaatgttt ataacgataa ggtaatgaag gaggaacttc ctgcatatgt
     1381 ttatgagtct ttgaaaaaga cgatagagca gggaggaccg cttgatgacg gtatagctga
     1441 tactgttgct gaaagcatga aagactgggc tattagaaaa ggtgctactc attatactca
     1501 ctggttccag ccaatgacag gtataactgc agaaaaacat gattcctttt taatgattag
     1561 aaatggcaaa ccggtaatgg aattaagaag caaggaactc attaaaggtg aacctgatgc
     1621 ttcaagtttt ccttcaggcg gattaagagc tacttttgaa gcaaggggat acaccacttg
     1681 ggatgcaaca tcatatgcat ttataaaaga caggacttta tatataccta ccgctttttg
     1741 ttcatactgc ggagaaccac tggataacaa gacaccgcta ttacgttcta tggatgcgct
     1801 taacaaacag gcgctaagaa ttgtaaggct ttttggaaat acaacagcat caagagtaat
     1861 acccgttgca ggatgtgaac aggaatattt tcttattgac aagaaacagt atgacaaaag
     1921 aaaagatttg cttatatgcg gaaggactct gtttggagca aaatcaccaa aagggcagga
     1981 attgaacgat cactattttg gtaatataag ggaaagagtc caaaatttca tgagagaact
     2041 tgacattgaa ttatggaaac ttggaataac agcaaaaaca gaacataatg aagcagctcc
     2101 tgctcagcat gaattagcac caatatatgc tacagttaat attgctgctg accataatca
     2161 gattattatg gaaatgatga agaaagtagc agagcatcat ggtcttgcat gccttcttca
     2221 tgaaaaacct tttgctaaca ttaatggttc cggtaagcat aacaactggt caatatctac
     2281 aaacgagggt gatatgcttt tgagtcctgg taaagaccca tcaaaaaata agagatttct
     2341 tcttatactt gcagctgtgc ttaaggcagt tgatgaatat tccggattac ttagacaaag
     2401 catagcaact gcaggaaatg accacaggct tggttcacac gaagcacctc caggaataat
     2461 ctcagtatat cttggtgcag aattaacaga aattctagag gatttagaga acggcagaac
     2521 agcacataat aaagcaagag aaaaattctg tatgagtgag actttaccag tattattcaa
     2581 ggatacatgt gatagaaaca ggacatctcc atttgctttt actggaaaga aatttgaatt
     2641 ccgtatgcca ggctcatcat gttcagtatc tgatgctaac actgttataa atacaatagt
     2701 agctaattct tttaaagaat ttgcagatat tttagagaaa tctgataatt ttgataagac
     2761 agttgatgat ttaataaaac agactgttct taagcataaa agaataatat ttaatggtaa
     2821 taattattct tcacaatggc ttgaagaagc taaaagaaga ggtcttcccg ttcttaatgc
     2881 aacaccagag gcagttgatt gtcttgttga agataagagt tttaaacttt ttgagacctt
     2941 tgggatattc tctaaaactg atttaatatc aaggcatgaa attaaaatgg aaaactactg
     3001 taaaattatt tctattgaag caagtactat gattgaaatt gcaaaaagag agattcttcc
     3061 gtcatcaatg gattatgcaa gtacgatcta tagcagcata cttatgaaaa aggatatcgg
     3121 actagacata tcgacagaaa cattaatagc tcagaatctt tctgataata ttaaaaattt
     3181 gaacgactgc attattgaac ttgaaaaagt ttcagcaaat acagaaaatg catatgattt
     3241 taaggacaag gtaatacctg tcatgaataa attaaggaag tatgctgata gtttggagtg
     3301 tattgtatca aaagattact ggcctatgcc gacctattct gatatgctct atagcgtgta
     3361 ataaattggt ttttataaat tttatatatt atttaatgta gctttatata attaacaaaa
     3421 ggatatgtaa atcatatcct tttattttat nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3541 nnnnnnnnnn tgtccagtgc ttcactgtca actcttatac ccattgaaga aatttccaaa
     3601 gcagaatcca gcactttgtt atacattaaa atgtctccat tcatttccca atcatcgtag
     3661 tctggtgctc tgccatcgtg cttttttcct gatttcaaag ttttacctat ctgcattata
     3721 aaaacagttt tatgttcttt aacatacaca tcttcccgct gtttaggact aagatcagga
     3781 tacatatctt caagttcctg agatgttata aacgacacat ttctagtaac tgttttttca
     3841 agagcaggga aaatatcatt aatcatttcg tgtgtatcgc atattgcact tactatgtcg
     3901 catacaatct gttttaagaa cggtaaagtt ctttcttgtt ttgtaataac tctttcccag
     3961 tcccactgat caacatagat agaatgaata ttatccatat cttcatcacg tcttattgca
     4021 ttcatatctg tgtatattcc ctctccagca ggaaagttat atctgtgtaa tgcaagtctt
     4081 ttccatttag caagtgaatg aactatttca gcttgcatat cattttcttt agtttcaaaa
     4141 caaacaggtc tttcaacccc atttaaatta tcattaagac ctgattgaga atcaacaaat
     4201 agaggagctg aaactctttt caagtttagc gctctacaaa ggttttcttc aaaaattctt
     4261 tttaatgaac caattgccag ttgggtctca tatatactta atttggattg ataatcttta
     4321 ggtatttgta tagaactcat gtttaacctg ccttgaaatt ttcctaaaag tctaccatac
     4381 catatgtatt gtgtcaatta atttctaatt tgcatagcat atttgtatat gataaaataa
     4441 ttctaatatt cggaggatga tatggcacaa aagaaaagaa catctgttag tcgtaaagca
     4501 gcagaaaata gtaataagca agtgcatagc aatgtgctta ctaaggagat atttggaata
     4561 gtaataattg cattaggctt actagctttt gtatgtgtat ttttcacaag taaatcaggt
     4621 tcatttggaa gaccatttaa acaagttaca gctggtttat tcggatgggc tcattattta
     4681 atacctcttg ctattcttct tttcggttgt tatattgcat tagcaggaag agttaaagta
     4741 ctaagtagga aaactgttta ccttatactt ttaatcctga ttgtcccttt attttttcaa
     4801 attgtatatt tgaaaaataa tatgtcagat atatcaaata acatgaatgt ttggcagttc
     4861 attgcacata tgtatattat cggaagagat aatatacata atggaggtgt tttcggaggc
     4921 attattatga ctcctctggt tattttcttt ggtacaacag gtgggtacat tattacaata
     4981 actgcaactg tagtattagt aatttatata acaaataaaa gttttttaga ctttcttatc
     5041 aaggtcaaga atatgttcaa agctatattc agttggatga aggatgcttt caaacctata
     5101 gatgatgatt ctgaaaagaa aacagtaaca actatatcga agcagccata tatacaggat
     5161 acaatagatg atttgcctca ggttaataaa gaaccaataa acattgtaat gaatcgaaaa
     5221 tccaaagata tgcctgagca gacacctaaa aaaccggtat caagatttac tggtccaata
     5281 attattaaaa aagaggaaga gaaaaaatcg actatgcctg ttccaaaacc ggaaaatgat
     5341 cagaagaaag gtgattatgt atttccacca ctatcacttc ttgattcata catggatagc
     5401 ggtgattctg ataattatga acagatagcc aggaaactgg aaaacacact gcttagtttt
     5461 ggtgttgaag tttcagtaac taatataatg tgcggaccta ctgtcacaag atatgaatta
     5521 cagccaaaat cgggagttaa ggtaagtaaa atagttgcgc ttgcagatga tatatcgtta
     5581 aatctggctt caagggacgt caggatagaa gcacccgtac caggtaaatc cgttgttggt
     5641 attgaagttc ctaatactaa aaagagtgtg gtttcactca aagaagttat cggttctaaa
     5701 gaatttcaag gatcaaagag cactcttaca attgcaattg gcaaagatat tgcaggaaat
     5761 tgtacagtag ctgatgtacg tagaatgcct cacatgctga tagcaggtgc gacaggttca
     5821 ggaaaaagcg tatgtataaa ctgtataata accagtatac tttacaaagc atcaccagat
     5881 gatattaaat tcattatgat tgaccctaaa aaagttgagc ttataaatta caacgggatt
     5941 cctcatcttc taataccagt aattactaat cttaagaagg ctgcaggagc attaaactgg
     6001 gctgtggaag aaatgagtaa caggtatgag atgttcaata aaagaggtgt tcgtgatata
     6061 aattcatata atgattcgat aaaaaatgat cccgagataa agaaaatgcc taatatagta
     6121 atcattattg acgaattagc agatcttatg atgactaatc ctggagaagt ggaagaatca
     6181 atatgcaggt tagctcagtt ggcaagagct gcgggaattc atttaattat tgcaacacag
     6241 agaccttctg ttaatgtaat aactggcgtc ataaaagcaa acataccctc aaggctatca
     6301 tttgccgttg taagccaggt tgactcaagg accatacttg atatgggtgg agctgaaaaa
     6361 ctgttaggaa acggagatat gctgtttcat ccaatgggac tgtctaaacc ggtaagaatg
     6421 caaggagcat atatatctga ttcagaaatt aataaagtag taaaattcat aaaagaaaga
     6481 tcttcatcag aatatgatga tgatgttgtt gacaatattg aaaaatcaaa taataaaaca
     6541 gcaagtactg atattgaaaa tgaaatagac gaattacttc cagctgctat tgatcttgtt
     6601 gttgaaatcg gtcaagcatc agtttcatat ttgcaaagaa aacttaagct tggatatggc
     6661 agagctgcca gaattgttga ccaaatggaa ctaagaggta tagtaggtcc tttcgaagga
     6721 tcaaaaccca gagcagtatt aatatcaaaa gatgagtggg cagaaatgca gatgagtaat
     6781 cctgaactgg atgtggaaaa gagcagatga gttttttacc aataagtaga gaagatatgg
     6841 aacagagaaa ctgggagtat attgatatac tctttgtgac tacagaagct tatgttgacc
     6901 atccttcttt cggtcaatca attattacca gattattgga ggataaggga tatcgtgttg
     6961 caatattgtc tgagccagat atttcttcat gtgaggattt taaaaggttt ggagaaccaa
     7021 ggctagctac tcttatatca ggaggagtaa ttgacagtat ggttaataat tatactgcaa
     7081 acagaaacag aagaaggact gactgttatg cttcaaaagg aaaaacccga ccagacagag
     7141 cagttatagt ctatagccaa agagctagag aagcttacaa ggatgttcca ataattatag
     7201 gcggtgctga agcctcgatg cgacgatttg ctcattatga ttactggcag aatgttgtga
     7261 gacgatctat aatatttgat gcaaaagcag accttttagt atatggaatg ggtgaaacag
     7321 ctattgttga gattatgaaa cttcttgaca agaatgttcc tatatccagt attacaagcg
     7381 taaaaggaac agcatatatt tcaaaagata taccaaaagc tcataaagta tttaaagaca
     7441 ttgttcttcc aacatttgaa gatatatcaa cagataaaat tgaatatgta aaagctttta
     7501 agatacagta tgaacaacag gatccaatat ggggagcaag acttattcaa ggacacaatg
     7561 atcgtttttt agtgtgcaat ccaatggctg acccactgtc agaggaaatg atggattata
     7621 tttactcatt accatatgaa agatcatacc atccaatata tgaagaatca ggaggtattg
     7681 attctttaaa agaagtcaag tactctgtaa caagccacag gggatgtttt ggtaactgtt
     7741 ccttttgctc aatagtactt agccaaggta gaataattca aagaagaagc agaaaaagca
     7801 tactagatga gattgaaaag cttacacacg agaatgattt tcatggatat atacatgatt
     7861 tatccggtcc tacagcaaac tttgttaatc cttcatgtaa gaaacagctt gataagggaa
     7921 tgtgcaagaa taagcagtgt ttgtttccaa caccttgtga tcaactggaa gtaagtcatg
     7981 aatatctttt agagacattg aaagaagcaa gaaaagtacc aggtgtcaaa aaagtattca
     8041 tgagatcagg attgagatat gactatctga tgcttgatag agataaatca gtattagaga
     8101 agatttgtaa atataatgtt agcggtcagc ttaaagtggc tcctgagcat gtaagtgatt
     8161 cagtacttga actcatggga aaacctcctt tttcagtata ttatgaattt aaggaatgtt
     8221 ttgaaaaggc aaatgataaa gcaggactta aacaatactt acttccatat ttcatatcag
     8281 gacatcctgg tgcaactatt aaagaaacga taaaactcac tgaatatctg attgaatcaa
     8341 aatttatacc agagcaggtt caagatttct atcctacacc tggaagttta tcaacctgta
     8401 tgtactacac aaagataaat cctttaacta tggaaaaagt atatgttcca aatgcccatg
     8461 acagacgggt agaaagagcg cttttacagt ttaacattcc agttaacaat aagattgcga
     8521 aggaaacgct aataagccat gggcgtaaag atttagcaga taaaataaca attagaatta
     8581 ggcaaaaaaa gaaagcaaaa aaatgaaaac atatagcata ataactcttg gatgcaaagt
     8641 aaatcaatat gaatcctctt caatcgctgc cagttttgac gcgatgggat ttgaatacaa
     8701 aaaatttcct gatatctgtg atatatatgt tgtaaataca tgtactgtca caaatatgag
     8761 tgacagaaaa tctagacaga tgatgcacaa agcaagatca ctaaatcctg actgtgtaat
     8821 aatagcaaca gggtgtcttg tacagaataa acctgaggat atgaaggcca atggtgctga
     8881 tattatattg ggaaataatg agaagccata tattgaagat attgttaata attatttaaa
     8941 caataaagaa gacagtttaa ttagtgtttc tgacatatcc agatataagc catatactaa
     9001 cacacgcttt gctgatagat gggaaagaac aaggggatat attaaaattc aagatggatg
     9061 tgataatttt tgttcttatt gtattattcc atatgtaaga ggcagggtgc gaagcaggga
     9121 gttaactgac attattaaag aagcagagtt catggcttcg aatggtataa aagaagttgt
     9181 acttacaggt atccatttga attcatatgg taaagatcat aatgaaatta gtttgctaga
     9241 tgtgatttct gctttgaatg atgtaaaagg tatcaggaga ataagactgg gatctttgga
     9301 accaaatgtc gttgatgaag aatttataaa tgaaatctta aaatatccaa agctctgtcc
     9361 gcattttcat atgtcactac aatcgggatc agacaatgtg ttaaaggata tgaataggag
     9421 atatgattct agaatatatt tgaaagcggt taaattgtta agagacaatc ttcctgatgt
     9481 tgaacttact accgatatta ttgtgggtta tccgacagaa acagaagaag atttcttaat
     9541 gagtataaat ttagtaacta aagcagagtt tcttaaaaca catgtcttca agttttcaaa
     9601 aagaaaagga acaaaagctg aaaaacttaa gccgctaaat agtatgatat tgaatgaaag
     9661 aagcgacagg cttatatcgg ttgctgacaa gacggcatat ttaaaaatgt catcttatat
     9721 aggaaaaaca ctcgagattt tagtagaaga acagaagcaa ggatatgtta tagggcatag
     9781 tcgtaattat attaaggtac aagcacatct taaaaaagaa acaaatacat cttatataaa
     9841 taatttttat aaggttttaa tcactaaagc agataaaaat aatctgtatg gcaatttggt
     9901 tgaatagtca aatgtcatat aaatgacgca ctttacatat tgtccacttc tttttatagt
     9961 ttaatcttga aattatatgc gatttgaagt aaactacata cataactgat gaaggagaca
    10021 tgctaagtgg ataaaggatc aacaataaat ctggactttc taaagagtca gcaaagcgaa
    10081 acaagagaaa ttcttaccct tgttttcagt gccatgacag aaaaagggta cgatccggtc
    10141 aatcaattgg ttgcatacct catgtcagga gatccaacct atataacaag ccataacgga
    10201 gctagaaacg ctattagaaa gttggaaagg gacgaaataa tggctgagct tattcagtat
    10261 tatttcacat caaaataatc ttaatgataa ttatcggaat agactatgga gagaaaagaa
    10321 tcggggttgc caaaagcgac cctttaggtt tattagctca tggcataact acaatagaat
    10381 gggataggga tattgaaaaa ccaataaatc aaattcagca aatagttatt cagcacaatg
    10441 ttgaaaagat tgttttagga cttcctttga acatggatga tactgaagga gagaaagcta
    10501 ttaaagttcg tgattttgca gatgttttga aaaaaagaat aaatatccca gttgagtttt
    10561 atgatgaaag attatcaaca gttgaagctc atgatataat tcacatgcag gggaagaaga
    10621 ctggaaagaa taaaaagaga atagatatgg tagcagcatg tgttatactg caatcatatc
    10681 ttgataatat taagaaaaaa taaggagata taaaatggat aacgagagag atgacataat
    10741 aatgatggaa gacgaaaatg gcaatcctgt agagatgata gcgctggaaa tgcttgatta
    10801 tgaggaaaaa acttacgttc ttatgcagac tactgatgaa acagatgaaa attcatatat
    10861 attcgaatat gaagaagaag atgatgatgc aattttgaca cctgtagaag atgataacct
    10921 ggtagataaa ctgtttgaat tgtttatcaa gaaaatggat atggattcag aggaagaaga
    10981 agaggaataa aaaatagatg tttgatttga tgaatgtcga aaaacccgcc agatacaccg
    11041 gaggggaatt caacgagata gtcaaagatc atgaaaagat gctttcgagc tatgcatttt
    11101 gctttcctga tacatatgaa gtcgggatga gccatcttgg aatgcgcatc ttgtatcatg
    11161 taataaataa cagagataaa acggtatgtg aaagggtttt tgctccatgg atagatatgg
    11221 aggagcaaat gaggtctcat aaaatacctc ttttttcttt agaaactaaa aagccagttt
    11281 ctgaatttga tatcgttgga ttcactttgc aatatgagat gagctacaca aatgtactta
    11341 atatgctgga cctttcaaac atacccataa gaagagaaga caggaatgaa acacatccaa
    11401 ttattatggc aggagggcct tgttcagtaa atcctgaaac tctgtatgat attatggata
    11461 tatttataat tggtgaagct gaggaagtta taaatgaggt tcttgattta tttgagttaa
    11521 tgaaaaaaga caattcatcg agaaatgact ttctaagaaa ggttaatgac attgaaggag
    11581 tatatgtacc tttattccat aatttgaaaa agaagataaa gcgcaggatt attaaagatt
    11641 tggacaaagt ttcatttcca actgattcaa tagttccata tataaatatt attcacgaca
    11701 gagcttcatt agaacttttc agaggttgta taagaggatg cagattttgt caggccggct
    11761 ttatttacag accagtaagg gaaaagaaag ctcagacgtt aatagaccag acaaaagata
    11821 ttctagacaa aacaggttat gatgaaatag gtatggtttc tttaagtact tcggattatt
    11881 ctgatttaaa aactttgtgt gacggcatgt tagagcatac agaaaaaagg atggtaaatc
    11941 tgtctgttcc ttctatgaga attgataatt tttcaattga ccttatgaaa aggatacaga
    12001 aagtaagaaa atccggttta acttttgctc cagaagccgg aagtcagaga ttgagggatg
    12061 ttataaataa aaatcttact gaagaagaga tacttaactc tgtaaaaata gcaagagatg
    12121 gcggatacga taatattaaa ctatatttta tgttaggttt accgacagaa acagatgagg
    12181 atgttttagc tattgctgaa ttaggaaaga aaattcttga aatatttggg agaaagagaa
    12241 ggataaatat tacaatatca acatcctttt ttattccaaa accatttaca ccgtttcagt
    12301 ggtgcgctca aataacaaaa caggaaatgg taagaagaca acggcttttg agagatagca
    12361 taaaagaaaa aaagattaaa tataactggc atgattttaa tacatctatc gttgaatctg
    12421 caatatcaag aggagacaga ttaacaggaa aagcaattat aagagcatgg gaaaaaggct
    12481 gtaagtttga ctcttggaat gaattctttg atatggataa atgggtcagc gcattttcag
    12541 aatgtggttt agatatatat cagcaggcat caagagagat taattcaaat gatatacttc
    12601 catgggatca tattgatatt ggagtaacaa agaagttcct gaaaaatgaa tataagaaag
    12661 cttttgatga aataactaca aaaaactgca gagaagaatg cactggatgt ggtatcgcta
    12721 aatataaatg tggagtatgc tttgaaaata attagattca aatttaaaaa aaatgaaaaa
    12781 gttatgtata tatctcatat tgaaatgatg aatgcattca caagagcact aagaagagca
    12841 gattttgatg tagtatttac acaaggattt aatcctggta tgaaactcgt ttttgcactc
    12901 ccattaccag ttggtgtttc atcagatagt gaatatgttg atatatggtt taaccaagat
    12961 tataaatgcc agtatgtaat agataaactt gcacaggctt taccgactgg gtttgaactt
    13021 cttgaagcaa actctgtaga aaaatccaca ttgatggaag atgtgggttc agcaatatac
    13081 ggttatattg ttgaatgtga taaaaatctt aaagaagcat tagataagct tatttctctg
    13141 gataaaattg aggtagaaaa atcaaataaa aagggaccag tagacattct gccatatata
    13201 tacaaaatag attataaaga tgaaacgagt ttttcagtat tatgtaaagc tggagcaaag
    13261 ggtaatcttc atccaagatt acttttaaaa gctttagaga aatatactga ttgcagaata
    13321 tatgagaaat ctatcaatag aaataaactt tttttgaaag ttcaatagat aaatttaaaa
    13381 acttgtttgt aaaacagacc aattaaattg tataattgcc atatggtaaa agattatgaa
    13441 cggctggatg atctccagct tgatgggtta aaacttatac agaacagaaa gcatttctgt
    13501 ttcggcactg atgcagtact tgtttgttca tttgcaaatg taaaaaatac atcttctgtt
    13561 attgacttag gtactggaaa cggtattata cctcttttac tgtatggaaa attcaaacct
    13621 agactgataa tgggtattga gatacaaaaa gaggcatatg aacttgcagt gcgaaacgtt
    13681 aaactgaata atcttgaaga tcgtatatcg attattaatg gagacataaa aaatataaag
    13741 gaatatttgc agtgcggctc gtttgatcat gttataacta atcctcctta tcttaaatcg
    13801 ggtactggta ttattagtgc agaagattct atagcatatg caagacatga gatactgtgt
    13861 acccttgatg atgttttaaa agcagccaga tatcttttaa aatctaaagg gacatttaca
    13921 atggtgcata gacctgacag actcaatgaa atacttatgc agatgaaaaa caatggtatt
    13981 gaaccaaaaa caataagatt tgttcatcca aatgaaaaat ctcaagcaat attggtacta
    14041 attcaaggat taaaagatat gagtccttct ttagtagtag atcatccgct tattatccat
    14101 aaaccagatg gttcatatac agaagaactt aatatgctat atgggaggga atcatagtgc
    14161 tatatttagt agctactccg attggaaatt tatcggatat ttccaaaaga gcgattgaag
    14221 ctcttgaaaa ctgtgactac atagcttctg aagatacaag gcataccttg aggcttctaa
    14281 atgcttttgg catcaaaaag aaactggtca gttatcatca gcataacatg gaaaaaagcg
    14341 gacaaagcat tcttaatgat gtcatgtctg gaaaagatgt atgcttagta agtgatgcag
    14401 gatgtccagc aatttcagat cctgggaatg aacttgtaaa attaatgcat aagaataatc
    14461 agaaggtttc
//
LOCUS       DAOSXM010000056        14210 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2111
            NODE_2708_length_14210_cov_11.837372, whole genome shotgun
            sequence.
ACCESSION   DAOSXM010000056 DAOSXM010000000
VERSION     DAOSXM010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864178
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 14210)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14210)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:24:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,261
            CDSs (total)                      :: 2,229
            Genes (coding)                    :: 2,218
            CDSs (with protein)               :: 2,218
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 3 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14210
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2708_length_14210_cov_11.837372"
                     /isolate="AMR_MDS_2111"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(43..1491)
                     /locus_tag="PLQ68_07650"
     CDS             complement(43..1491)
                     /locus_tag="PLQ68_07650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015254409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HXK72565.1"
                     /translation="MSFKVAFIGAGSIGFTRQIVKDLLSVEEFRNIEISFMDISEHNL
                     EMVRALCQRDIDSNKLSIKIEATTNRKEALKNAKYVFVTVRVGGLEAFATDVEIPLKY
                     GVDQCVGDTLCAGGIMYGQRGIPVMLGICSDIKEVAREDCMILNHTNPNAMITWAANH
                     YGKVPTIGLCHGVQGGHQKIADVFGLKKEEVDIICAGINHQTWYTQIRHNGEDLTGKL
                     LDAFLNHEEFSKTEKVRIDMLKRFGYFSTESNGHLSEYVAWYRKRLDEINDWIDLSTW
                     INGETGGYLRVCTEKRNWFETDFPEWMKKDPFVYDKEHRGHEHGSYIIEGLETGRLYR
                     GHFNVVNDGCITNLPDDAIVEVPGYIDANGVNIPKVGALPLGCAAVCNSSISVQRLAV
                     HAAVEGNELLLKQAMMMDPLVGAVLNPNEIWQMTDEMLVACEDLLPQYKDVIKAAKER
                     LLHKEIKTDDSFKGAARLKVKTIEELKEENKK"
     gene            complement(1545..2186)
                     /locus_tag="PLQ68_07655"
     CDS             complement(1545..2186)
                     /locus_tag="PLQ68_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010243809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HXK72566.1"
                     /translation="MKKLYEYTFGEEVANTITHGVMAFLVLLSMPFAILYVNAKGRLI
                     DAIGVSIFMISIFLMLLSSTLFHSMEKDSKHKRIFKIFDHIFIYVAIAGSYTAIALSV
                     IGGWQGIVITCIQWTMVIVGILQKVLFDRKNPRSSVAVYLIMGWTLLAFLPLFIKQAS
                     VGIFVFVLLGGIFYSVGAIIYAKKSFKYYHMVWHIFVNFGALMHFIGLVFFLQ"
     assembly_gap    2278..2377
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2378..3084)
                     /locus_tag="PLQ68_07660"
     CDS             complement(<2378..3084)
                     /locus_tag="PLQ68_07660"
                     /inference="COORDINATES: protein motif:HMM:NF012904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HXK72567.1"
                     /translation="MTKSINSGVWPTMITPFTNEDKIDYKGLDSLIDWYIEGQVDGLF
                     AVCQSSEMYYLTQEERISLAKFVVEKVNGRVPVVASGNFSKDIYAQAEEIKMMADTGV
                     DAVVILTNGLADKDDKHDVWLKNAEIILNNIPKDIQLGLYECPVPYKRLLTLDELKWC
                     ADSNRFLFLKDTCCSEDILKDRLRILKNSNLKLFNANAATLLYTLNLGGAGFSGVLAN
                     FFPKILKKLVDTQKKDKA"
     gene            complement(3104..4093)
                     /locus_tag="PLQ68_07665"
     CDS             complement(3104..4093)
                     /locus_tag="PLQ68_07665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72568.1"
                     /translation="MISNNETNTDYEYPKFRFDLVYKDPVFDISPHSARMAACYKDYA
                     GTYHLFTDYIDAKLNTVASWQAEIVYYRSRDLLSWEYVSTAVEKGNSPDMPDSYGVAS
                     SEILCFKDYLYLFYSARQNPLNSDIGNPLAKPGETGYVSTEIMYAKALTDKNKAPILP
                     FIKQGFAIKREHDWESMRIDDPCVVVDNKKIYMYYKGFCDNIDRNNLKLGFAEADIDD
                     MRFKKYGFPILEVHSGLEMSRVFRYKDKWIMFLRHFERQTGYSWRQYVSKDGIDWKMY
                     DPYLFNCHGGIENSGAADIMIIKDVDNNFTGKVLACDMDDNVLKLWLYECKEI"
     gene            complement(4098..5351)
                     /locus_tag="PLQ68_07670"
     CDS             complement(4098..5351)
                     /locus_tag="PLQ68_07670"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HXK72569.1"
                     /translation="MEILADNFIKIYESENPLVIGCYSPGIIRLESGRLIATIEIGGK
                     KTHPIEGPKIESDLGQSQGKVFTSDDKGQTWQHRADYAIAHARPFVAGKSVYILGQAG
                     DLNIIKSDDDGVTWTEPVKLTNNEHWHQAPCNVHYANGNVYIVMEKKVYNDINGWNVN
                     GVAPILMRANVNDDLTKRESWVFASELAFRDLIDEKDLDYFGVPFYETPRRTFIEVSK
                     GRPCHPIGWLETNIVQFVDPDHIWYDETGHTFHLWMRAHTGKTGFACIVKVIETNYNT
                     MVTIPEKAPSGKKIVYVPCPGGQMKFHILYDEVTKLYWLLSTQATDSMTKPERLPKER
                     YNLPDNERNRLQLHFSKNCIDWCFAGMVSIGKGIKHSRHYASMVIDGEDLYVLSRSGD
                     ENARDPHNGNFISFHTIKDFRKLIY"
     gene            5521..6114
                     /locus_tag="PLQ68_07675"
     CDS             5521..6114
                     /locus_tag="PLQ68_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009428465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive phage infection protein"
                     /protein_id="HXK72570.1"
                     /translation="MNKSSELEKIVNRNNGYLISSEAINEGISKQQLYSYIKETNMEK
                     VAHGIYVSEDTWVDHLYLIHLRNSNAVFSHETALFLHGLMEREPSQITVTVKFGYNAS
                     HLVNNGIRVITAVDNYFSIGRNKTKTNFGNTVDVYDMERTICDIIRNKKDMDPQVYQT
                     AIQEYMKSSEKKLPVLMRYASIFRIDNIVRTYTEVML"
     gene            6111..6959
                     /locus_tag="PLQ68_07680"
     CDS             6111..6959
                     /locus_tag="PLQ68_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009537672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HXK72571.1"
                     /translation="MIKTATQLKAKIRNLSDGNSLKAQALIRNYIMERFLERISLSDY
                     RDNLILKGGMLVSSIVGLETRTTMDIDTTVQSLLVTMSEAKRIIEAIISVALEDNVSF
                     HIKKIEQIMEDFDYPGIRFILEAGLDNLRQVIKIDLSTNDVIIPSAIVYQYRLMFEDR
                     NISLKAYNLETLLGEKIETVISRGTANTRMRDFYDIHTISTHKTERIDYEILHKALFT
                     TSKKRNTLDLIEDYKRIIDEVRRSGVMKKNWDNYSKDLLFYENITWIQVVDSLVILLQ
                     KISIYK"
     gene            complement(7036..7224)
                     /locus_tag="PLQ68_07685"
     CDS             complement(7036..7224)
                     /locus_tag="PLQ68_07685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72572.1"
                     /translation="MANGIEIFREYFSKYKEQYTLIGGMVCNLLMSDAELDFRQTKDI
                     DMVLIVEAMTNEFASSCD"
     gene            complement(7229..8200)
                     /locus_tag="PLQ68_07690"
     CDS             complement(7229..8200)
                     /locus_tag="PLQ68_07690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72573.1"
                     /translation="MIEYLQKIFGAKIKLSDYRLPKNTPIYLQEGYTVNKLIWDNSEC
                     VVITPLDVAMRLPVLKKHYQMIKDICNLPCALNLEQLTALQRENLISNNIPFISIKQQ
                     IYLPFWGSIFLERLKKEAKVPKKMTPTTQLVFLYIFYRLISDERKINSTSISFELCLP
                     KSSVSRAVTELEAYGLLMMYSEGTKKWLSFKSNSNATLTKAIGYMCSPISRIVYLKEL
                     EKGLCYKYGGIKALSMMSILSANERDGAIVFNKNEIKKISEKNIISERTFDDFGGVIT
                     EVWRYDPGLLTDNEIVDDISLLLCLQDNEEERIQKELDKIRIKYGMR"
     gene            complement(8437..11400)
                     /locus_tag="PLQ68_07695"
     CDS             complement(8437..11400)
                     /locus_tag="PLQ68_07695"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA family protein"
                     /protein_id="HXK72574.1"
                     /translation="MLFAVDYNDCRVRIDETHSNKEYFCPYCGAPLITKKGDIRQHHF
                     AHKSHHLCSDTWEQSHSYDISLWHDEWQSRFPAVNQEIRLSLGETKHRADILIDRAVI
                     EFQHSIMPVKAFDDRNNFYSNLGYKVIWLFDLSDLIDKGNISYSNTDNGLEFSWKNPK
                     KAFNSYDVKTGCIDLFFQINDNDIKDPCIVKVTDVSESGFEVFYSSSLMSKNDFLEYV
                     GLNNGECAAPYIDDLESNKRYLRFKEKHKIKLNKQQERALQAIEGSNLLLAVPGSGKT
                     TVLVSRLGYMIKEKSINPKSILAITFSKNAAEEMKQRYITFFGEESGNIIDFRTINSL
                     SLDIYKRYCKSAEISERKLIEGKELHTLYGKIYNQFHGDEYASESDILEFATEVSYTK
                     NMMLSDEQIIEFVSDYPMFKKMYDAYMKWLKDNNRMDFNDQMVFAHWILKNSINESSN
                     WQRRYKYICVDEAQDTSKIQHEIIRILAKENNIFMVGDEDQSIYGFRAAYPKALLNFR
                     IDYKNPYILRMERNYRSTTQIVEKAQAFIAKNKGRYEKNMTAERGNGEPVELIRVASV
                     EEQYRKILDIAKTENDEIAFLYRDNESSVVLIDLFLRNQIDFNLKKPEMNFFGHKVVL
                     DIIAYLNIAMNPFDGRYFNQVCNKGIIFLKRKPKEYAIQNCKNRKISIYDALDEQMKY
                     VSNNKKDSANDFKSIMKNIVNKTSEDAINILLDNGYEKYIEKNSLDFNKINILQLLAK
                     QEPNIKKFIVRLSELEKQISNGFQNKENCKITLSTIHSSKGLEYDTVYMVDIYDGRFP
                     SSSKNYLSRSKDNADAEQEERRLFYVGITRAKNRLALFSIENKPSTYIDEIFPEEKVA
                     RISAEDEKRRILLAKFDKKERIHQRKTQYATELAFKLQKENQYKYEKLQEEKRKTMEE
                     SKKKEAEQAYNDHYNEVKDLFVQQKTPIRDSKGDRWVQCEYCGKISLSDEFIYYGGAD
                     HVNLGTCYKCSAKGR"
     gene            complement(11404..11619)
                     /locus_tag="PLQ68_07700"
     CDS             complement(11404..11619)
                     /locus_tag="PLQ68_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006440598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HXK72575.1"
                     /translation="MNSHLKLKNRLKEFRQEKGLTQTQLADVVGSSKNTISSIETGQF
                     CPTAYLAALLCIALDRKFEELFYFEKE"
     gene            complement(11616..11939)
                     /locus_tag="PLQ68_07705"
     CDS             complement(11616..11939)
                     /locus_tag="PLQ68_07705"
                     /inference="COORDINATES: protein motif:HMM:NF039453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6442 family protein"
                     /protein_id="HXK72576.1"
                     /translation="MKNKDILMAAQKEKSKGKEFENKQSVKSSLIGSLIALLVGIMLF
                     LLEYFINSSVNIGLIAVGLTAAAVQSLFEGIKNRKVHMIIIGVVEALVTLFAILAFVS
                     QLVTA"
     gene            complement(12559..>14210)
                     /locus_tag="PLQ68_07710"
     CDS             complement(12559..>14210)
                     /locus_tag="PLQ68_07710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014314895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HXK72577.1"
                     /translation="VIIGAVAAGTSAAAKARRNSEELEIAVYEKDTYISYSGCGMPYY
                     IGGEVESADALTPRDPYFFRSKYNVDILIGHEVLSINPKEKKLEVKNLFTEEVFFDYY
                     DKLIIATGAKSVIPPIRGADNTNVFTLRNINDMNKIKEYIAGKRPKTAVIVGTGFIGL
                     EVCENLKKLGIEVTMAERLPQVTPGLDGDMAVYVEEHLKANGVEVITNALVTEITDTS
                     VMLANGRSILADMILLSTGVRPDTALAKKAGIELGISGAIKVDKKMRTNVEDIYACGD
                     CIEQFSVINGKPLYRPLGSTANKTGRIAGDCATGGSLEFRGILGTGIFKIFDLAVAQT
                     GLSEREARDQGYDVVVCHNIKPNKPEYIGGKEIVIKGIADKVTGLLLGVQIIGYEGVD
                     KRIDVFATAITFKAKAEDLFHLDLAYAPPFATTKDPVMYTGMILDNILSRGRKLITAQ
                     ELEALIKSEEKYQLIDTRVKKQYEKGHIRTAINIPHTDFRAAIPQLDKEVVTVTYCNK
                     GTTGNATQNILINHGFKRAYALSGGFKQYSIVFLKEKTSLK"
BASE COUNT         4295 a         2808 c         2066 g         4941 t
ORIGIN      
        1 attaagggtg gcaatatgtt ttgtgggata tatcagtcta agctatttct tattctcttc
       61 tttaagttcc tctattgttt taacttttaa tctggcggct cctttaaagg aatcatctgt
      121 ttttatttct ttatgaagta gtctttcttt ggcagccttt atcacatctt tgtattgagg
      181 aagtaaatcc tcgcatgcga ccagcatttc atctgtcatc tgccagattt cattgggatt
      241 taaaacagcc cctaccagag gatccatcat cattgcctgt ttcagtaata gctcattacc
      301 ctcaacggct gcatgtactg ctaaacgctg aacagagata ctgctattgc acactgctgc
      361 acaccctaaa ggcaaagctc ccaccttagg gatatttact ccgttggcat ctatgtatcc
      421 cggtacttct acaatggcat catcaggaag atttgttatg catccatcat ttactacatt
      481 aaagtgtcct ctgtaaagtc tgcctgtttc cagtccttca ataatatatg atccatgttc
      541 atgtcctcta tgttctttgt catacacaaa agggtctttt ttcatccatt caggaaaatc
      601 tgtctcaaac cagtttctct tttctgtaca gactcttaga tatcctccgg tttcaccatt
      661 gatccaggtt gataaatcaa tccagtcatt gatttcgtct aacctttttc gataccaggc
      721 aacatactca cttaaatggc cattggattc ggtactaaag taaccaaatc tttttaacat
      781 atcaatacgc actttttctg tcttcgagaa ttcttcgtga ttcaaaaatg catcaagcag
      841 cttgcctgtc aaatcttccc cattatgtct aatctgcgta taccatgtct gatggttgat
      901 tccagcacaa atgatatcca cttcctcttt ttttaatcca aatacatcag caatcttctg
      961 atgtccacct tgtactccat gacaaagacc aatggtcggc acttttccgt agtggttagc
     1021 tgcccatgta atcatggcat taggattagt atgatttaaa atcatacaat cttctctagc
     1081 cacttctttg atatcggagc aaatacccag catgacagga atacctcttt gtccgtacat
     1141 gatgcctcca gcgcataaag tgtctccaac gcactgatcc actccatatt ttagtgggat
     1201 ttctacatcg gtggcaaaag cttccagtcc ccctactctt actgttacaa aaacatattt
     1261 ggcatttttt aatgcttctt ttctgttggt tgtggcctct attttaatgg aaagcttatt
     1321 actgtcaata tcacgttgac acaatgctct gaccatttct agattatgct cagatatatc
     1381 catgaaggat atttcaatgt ttctgaactc ttccacagac agaaggtctt ttactatctg
     1441 ccttgtgaat cctatacttc ccgcaccgat aaatgcaact ttaaatgaca taatcccacc
     1501 tcaatttaat ttattttttg ccttttcaag acacatacac tggtttattg taaaaagaac
     1561 actagcccta taaaatgcat cagtgctcca aaattaacaa atatatgcca gaccatatga
     1621 taatacttaa atgatttctt agcatatata atagcaccga ctgaatagaa tatgcctcct
     1681 aaaaggacaa agacgaagat gcccacactt gcttgcttaa tgaataatgg taaaaaggca
     1741 agcaatgtcc aacccataat aagatagacc gctacagaag aacgaggatt ttttctatca
     1801 aacagcactt tttgtaaaat ccccacaata accattgtcc actgtatgca ggtgatcact
     1861 ataccctgcc atcctccaat gactgataaa gcgatagcgg tataactgcc tgcaattgca
     1921 acataaatga atatatgatc aaatattttg aagatccttt tatgttttga gtctttttcc
     1981 atagaatgga atagtgttga ggatagtagc atcaagaata ttgatatcat aaagatagaa
     2041 acgccgattg catcaattaa cctgcctttc gcattcacat aaagaatcgc aaagggcatg
     2101 gaaagaagaa ctaaaaaggc catgacacca tgtgttatgg tattagctac ttcttctcca
     2161 aatgtatatt catatagttt tttcatcgat tgctcccgac tttcatatat atattataca
     2221 catttttatg ataatgcact tatatagcat caaaaatcat tcaataaaaa acaaatannn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngct ttatcttttt tctgagtatc
     2401 tacaagcttt tttaaaatct ttggaaagaa gttagcaaga actccggaga atcccgctcc
     2461 acccaaattc aatgtataaa gcaaagtcgc cgcattggca ttaaataact taagattgct
     2521 gttcttaagt atccttaatc tgtctttcaa tatatcctca ctgcagcatg tgtcttttag
     2581 aaacaggaat ctattagaat cggcacacca tttcaattca tccaatgtta aaagtctttt
     2641 gtagggaaca ggacattcat ataacccgag ctgaatatcc ttaggtatgt tgtttagtat
     2701 tatctcagca tttttaagcc atacatcatg tttatcatct ttgtctgcaa gaccgtttgt
     2761 aagtataacc acagcatcaa caccagtatc tgccatcatc tttatttctt ctgcctgagc
     2821 ataaatatcc ttgctgaaat tgcctgatgc aacaacagga actctgccat ttaccttttc
     2881 tacaacgaac tttgcaaggc ttattctttc ttcctgagta aggtaataca tctcactgga
     2941 ctggcatact gcaaataatc cgtcaacctg accttctatg taccagtcta taagggaatc
     3001 aagtcccttg taatcaatct tgtcctcatt ggtaaatggg gtaatcattg taggccatac
     3061 tccgctgtta atgctttttg tcatactttc tcctcttatc tttctatatt tccttgcatt
     3121 catataacca cagtttcaaa acgttatcat ccatgtcgca tgcaagcact tttcctgtaa
     3181 agttgttatc cacatcttta ataatcataa tatcagcagc acctgaattt tctattcctc
     3241 catgacagtt aaacagatat gggtcataca ttttccagtc aattccatct tttgacacat
     3301 actgacgcca tgaataccct gtttgccttt caaaatgtct taaaaacata atccatttgt
     3361 ctttgtatct gaatactctg gacatttcca gtcctgaatg tacttcaaga ataggaaatc
     3421 catatttttt aaatctcata tcgtctatat cagcttcggc aaatcctaat ttcagattat
     3481 ttctgtcaat gttatcacaa aaccctttgt aatacatata gattttttta ttatccacga
     3541 ctacgcaagg atcatcaata cgcatgcttt cccagtcgtg ttccctttta atagcaaaac
     3601 cctgctttat aaacggaagt attggagctt tattcttatc agtcaatgcc tttgcataca
     3661 ttatttctgt tgaaacataa cctgtttctc ctggttttgc cagcggatta cctatatcac
     3721 tatttagggg attttgcctt gctgaataaa aaagatataa ataatcttta aaacacaaaa
     3781 tttccgaact tgccacaccg taactgtcag gcatatctgg tgaatttcct ttctcaacag
     3841 ctgttgaaac atactcccaa cttaaaagat ctctgcttct gtaataaact atttcagcct
     3901 gccatgaagc aacagtatta agttttgcat ctatataatc agtaaaaaga tgataagtgc
     3961 ctgcatagtc cttatagcat gcggccattc ttgcgctatg aggcgatata tcaaaaactg
     4021 gatctttata taccaggtca aatctgaatt ttggatattc ataatctgta ttagtttcat
     4081 tatttgaaat catagcatta ataaataagc ttcctgaaat cctttattgt gtggaaagat
     4141 ataaagttac cgttatgagg atctctagcg ttctcatcac cggaacggct aaggacatac
     4201 aaatcctccc cgtctataac catacttgca taatgtctgg aatgctttat acctttgcct
     4261 atcgatacca ttccagcaaa acaccagtct atgcagtttt tggaaaaatg cagctgcaat
     4321 ctgtttcttt cattatcagg aagattgtat ctttcttttg gaagcctctc cggtttagtc
     4381 atgctgtctg tagcctgagt gcttaacagc cagtaaagct ttgtaacctc atcatacagg
     4441 atatggaatt tcatctgtcc gccaggacaa ggaacatata ctatcttttt gcctgaaggc
     4501 gctttttcag gtatggtaac cattgtattg tagttggtct caataacctt aacaatacat
     4561 gcaaatcctg ttttcccagt atgtgctctc atccatagat ggaatgtatg gccagtctca
     4621 tcataccaga tgtggtcagg gtcaacaaac tgcactatgt ttgtctccag ccatccaatg
     4681 ggatgacatg gtcttccttt ggaaacttca ataaaagtcc ttcttggtgt ttcataaaaa
     4741 ggtactccga aatagtctaa atccttttca tcaattaaat cacgaaaagc cagttcagat
     4801 gcgaataccc atgattctct tttagtaagg tcatcattta catttgccct cataagtatc
     4861 ggagcgacac cattaacatt ccatccgttt atatcgttat ataccttctt ttccataaca
     4921 atatatacat taccatttgc ataatgcaca ttgcatggag cctgatgcca gtgttcgtta
     4981 tttgtaagct ttacaggctc agtccatgtc acaccatcat catcggattt aataatatta
     5041 agatcccctg cctggcccaa tatatataca gacttacctg caacaaatgg tcttgcatga
     5101 gctattgcgt aatccgccct gtgttgccat gtttgtcctt tatcatcaga tgtgaatacc
     5161 ttgccctgcg actggccaag atcagattct atcttcggcc cttcaatagg atgtgtcttc
     5221 ttcccgccaa tttctattgt tgcaataagc ctcccgcttt ctaatcttat tattcctgga
     5281 ctataacacc ctatgaccaa aggattctct gattcataaa tctttataaa attatctgca
     5341 agtatctcca ttattacccc caaaacatat taaacagctt ttctgttatt atactatacc
     5401 tgtaagtgat gtgctaatgc ttctcattga ataaatcaat agtgccatat aatgatattt
     5461 aagtatcttg actatttcgc taaattaata tatcatattt agcgtaaagg aagtgtttat
     5521 ttgaataagt catctgaact cgaaaaaatt gtgaacagaa ataatggata tttaatttcc
     5581 tctgaggcaa tcaatgaagg aatttcaaaa caacagctat attcatatat taaagaaact
     5641 aatatggaaa aagtagcgca tggtatatat gtttctgaag atacctgggt agatcattta
     5701 tatttaatcc accttcgcaa tagtaacgct gttttctcgc atgaaacagc actttttctt
     5761 cacggactta tggaacgcga accgtcacaa attactgtta cagttaagtt tggttacaat
     5821 gcttcccatt tagtaaataa cggtatcaga gtaataactg ctgtagataa ctatttttct
     5881 attggcagga ataaaactaa aaccaatttt ggcaatactg tggatgttta tgacatggag
     5941 agaactatat gcgatattat tcgcaataaa aaggatatgg acccgcaggt ctatcaaaca
     6001 gctattcagg aatatatgaa aagttccgaa aaaaaattac cggttcttat gcgatatgca
     6061 tccattttta ggattgataa tatcgtacgg acatatactg aggtaatgct atgattaaga
     6121 ctgctacgca attgaaagca aaaatcagaa acttatcaga tggcaatagc ttaaaagctc
     6181 aagctctgat ccgcaattat ataatggaac gatttcttga acggatttct ttgtctgatt
     6241 atagagataa tctcatatta aaaggcggta tgcttgtttc atctatcgta ggtttagaaa
     6301 caagaacaac aatggacatt gatactacag tccaatctct tcttgtcaca atgtctgaag
     6361 caaaaagaat aattgaggca attatttcag ttgcacttga ggataatgta tcctttcata
     6421 ttaaaaaaat tgaacagatt atggaggatt ttgattaccc tggaatccgt tttatacttg
     6481 aagcaggttt ggataattta agacaggtta taaaaataga cctttcaaca aatgatgtga
     6541 ttattccatc tgctatagta taccaataca gattgatgtt cgaagataga aacatatcat
     6601 taaaagcata taatctggaa acactgcttg gtgaaaaaat agaaacagtt atttctagag
     6661 gtacagcaaa tactagaatg cgtgacttct atgatattca taccatttca actcataaaa
     6721 ctgaaagaat agattatgaa atacttcata aagctttatt cactacaagt aaaaaaagaa
     6781 ataccctcga tttgattgaa gattataaga gaatcattga tgaagttaga agaagtggag
     6841 taatgaaaaa gaactgggat aattattcaa aagacttatt gttttatgaa aacataacat
     6901 ggattcaagt agtagacagt ctggtcatac ttttacaaaa aataagtata tataagtagt
     6961 tattctttct tatttagctt ttataagaat tttatgccaa ttaaatttcc tattgaataa
     7021 cattattctg tgtgattaat cacatgaaga ggcaaattca tttgtcatag cttctacaat
     7081 tagaaccatg tcgatatctt ttgtctgtct gaaatctaac tcagcgtctg acataagcaa
     7141 attacatacc ataccaccaa ttagtgtgta ctgctcttta tatttagaga aatattctct
     7201 aaatatttca attccatttg ccataatctt atctcattcc atattttata cgtatcttat
     7261 caagttcttt ctgaattcgt tcttcttcat tatcttgcag acacagcaat agcgatatgt
     7321 catcaactat ttcgttatct gttaatagtc ccggatcata tctccatact tcagttatta
     7381 cacctccaaa gtcatcaaat gtccgttcgc tgattatatt cttttctgat atttttttaa
     7441 tctcattttt attaaataca atagctccat cacgctcatt agcactcagt attgacatca
     7501 tcgatagggc ttttattcct ccgtatttat aacataaacc tttttccagt tcttttaaat
     7561 agacaattct acttattggt gaacacatat aaccaattgc ttttgttaga gttgcatttg
     7621 aattgctttt aaagcttagc catttcttcg ttccctctga atacatcatt agaagcccat
     7681 aagcctcaag ttccgtaaca gctcttgata cactggattt tggtaaacat aattcaaatg
     7741 aaatactggt tgagttaatt ttccgttcat cacttattaa tctataaaat atatacaaaa
     7801 aaacaagctg agtcgtaggt gtcatttttt tcggaacctt agcttccttc ttcaatcttt
     7861 caagaaaaat gcttccccag aatggcagat aaatctgctg ttttatagaa ataaatggaa
     7921 tattatttga tatgaggttt tcgcgttgta aagctgtaag ttgttcaagg ttaagagcac
     7981 atggaagatt acatatatct tttatcatct gataatgttt tttcaaaact ggcaatctca
     8041 tagccacatc taatggagtt ataaccacac attcactgtt atcccatata agcttattta
     8101 ctgtatatcc ctcttgaaga tatataggag tattcttagg cagtctatag tctgatagtt
     8161 taatttttgc accaaagatc ttctgaagat attcaatcat agcattacct acttgttcca
     8221 taattattta taatgtacca tattatttgg aactattcaa gtaaatgtgg aacaatacaa
     8281 gtttatatat atgttgatgc agattacaaa ccgacactgt gtgtcggttt atctttacat
     8341 tttgtcggtt taaattaatc aaaatatgaa tttatagaat acctaattaa gatccaattt
     8401 ctatcgtttc tgtttatcag ataatatata tcgaaatcat cttccttttg ctgaacattt
     8461 ataacaagtt ccaagattca cgtgatccgc accaccatag tatataaact catctgagag
     8521 agaaatcttg ccacaatact cgcattgaac ccaacgatca ccctttgagt ctcttattgg
     8581 tgttttctgc tgaacaaaaa ggtctttaac ttcattatag tggtcattat aagcttgttc
     8641 tgcctccttt tttttagact cctccattgt tttgcgtttt tcttcctgta atttttcata
     8701 tttatattgg ttctcttttt gtagtttgaa tgcaagctcg gttgcatatt gtgtttttct
     8761 ctggtgaatt ctttcttttt tatcaaactt agcaagtaat attcttcttt tctcatcctc
     8821 ggcactaata cgggcaactt tttcttctgg aaatatttcg tcaatgtatg tacttggttt
     8881 attctcaata ctgaacagag caagtctgtt ctttgctctt gttataccga cataaaacaa
     8941 tcttctctcc tcttgttctg catcagcatt atcctttgat cttgacaaat aatttttact
     9001 tgaagaagga aaacgcccgt cataaatatc caccatatat actgtatcat actcaagccc
     9061 tttgcttgaa tgaatagtag aaagagtaat tttacaattc tctttatttt gaaatccatt
     9121 actaatttgt ttttcaagtt cagaaagacg gactataaac ttcttgatat tcggttcttg
     9181 ctttgctaga agctggagaa tatttatttt attgaaatca agagagtttt tctcaatata
     9241 tttttcatat ccattatcaa gcagaatatt gattgcatct tcagatgttt tatttacaat
     9301 atttttcata atagatttaa aatcattcgc actatccttt ttattgttgg aaacatattt
     9361 catttgttca tccaacgcat cataaatgga tatttttctg ttcttacaat tttgtattgc
     9421 atactctttt ggctttctct ttaaaaaaat gattccctta ttacaaactt gattgaagta
     9481 ccttccatca aacggattca ttgcaatgtt aagatatgca ataatatcaa ggactacttt
     9541 atgcccgaaa aaattcatct caggtttttt gagattaaaa tcgatctgat tacgaagaaa
     9601 cagatctatt aaaacgacag agctttcgtt atcacgatac aaaaaagcta tttcgtcgtt
     9661 ttctgttttc gcaatatcaa gaatttttct atattgttcc tccacagaag ctacccgaat
     9721 taattcaact ggttcaccgt ttcctctctc tgccgtcata tttttttcgt atctaccttt
     9781 atttttagca ataaaagcct gcgctttttc aactatttga gttgttgagc ggtagttacg
     9841 ttccattcgc aggatatagg gatttttgta atcgattcta aaattcaaaa gtgctttagg
     9901 gtaagcagca cggaatccat aaatgctttg atcttcatct ccaaccataa atatattatt
     9961 ttcttttgcg aggatgcgaa taatttcatg ttgtattttt gaggtatcct gtgcttcatc
    10021 aacacaaata tatttgtatc gtctttgcca gtttgagctt tcattaatac tattttttaa
    10081 aatccagtga gcaaaaacca tttgatcgtt aaaatccatt ctgttattat cctttaacca
    10141 cttcatgtat gcatcataca ttttcttgaa cataggatag tcagaaacaa attctataat
    10201 ctgctcatca gatagcatca tgttctttgt ataggaaact tcagttgcaa actcaagaat
    10261 gtcactttct gaagcatatt catcaccatg aaattgatta tatattttac catataaggt
    10321 gtgtaactct tttccttcta tcaattttct ctcgctaatt tcagccgatt tacagtatct
    10381 cttataaata tccaatgata agctattaat cgttctaaaa tcaattatat tacctgactc
    10441 ttctccaaaa aacgttatat acctttgctt catctcttca gcagcatttt tactaaaggt
    10501 aatggctaga atgctttttg gatttatgct tttttctttt atcatatatc caagccttga
    10561 tacaagcaca gttgttttgc ctgatcctgg aactgcaaga agcaaatttg agccctcaat
    10621 cgcttgaagt gcacgttctt gctgtttgtt gagtttaatt ttatgctttt ctttaaaccg
    10681 aaggtatctc ttatttgatt ccaaatcatc tatgtatgga gcagcacatt ctccattatt
    10741 taagccgaca tattcaagaa agtcattttt actcatcaat gaagatgaat aaaacacttc
    10801 aaatccagac tcagaaacat cagttacctt tacaatacaa ggatctttaa tatcattgtc
    10861 attgatttga aaaaacaaat ctatacaacc agtttttaca tcataactat taaatgcttt
    10921 tttggggttt ttccatgaaa attccaaacc attatcagta tttgagtaac taatattacc
    10981 tttatcaatt aagtctgata aatcaaacaa ccatataacc ttgtacccaa ggttggaata
    11041 aaagttgtta cgatcatcaa aagccttcac aggcattata ctgtgttgaa actcaataac
    11101 cgctctatca atcaaaatat cagcacgatg ttttgtttca ccaagcgata atcttatttc
    11161 ttgattcacc gcaggaaagc gactttgcca ctcatcatgc cagagtgaaa tatcataact
    11221 atgactctgt tcccaggtat cagagcaaag atgatgtgat ttatgtgcaa agtgatgctg
    11281 tcgtatatca cctttttttg taatcaaggg agcaccacaa taaggacaaa aatactcctt
    11341 gttactatga gtttcatcga ttcgaacacg gcaatcatta taatcaacag caaataacat
    11401 tacttactcc ttttcaaaat aaaaaagttc ttcgaatttt ctatcaagag ctatgcataa
    11461 taaagctgca agataagcag tcgggcaaaa ttgaccggtt tcgatagaac taattgtatt
    11521 tttcgatgat ccgacaacat cagctagttg tgtttgagtt aaaccttttt cctgtctgaa
    11581 ttcctttaat cgattcttta atttaagatg cgagttcatg ctgtcaccaa ttgactgacg
    11641 aatgcaagaa ttgcaaatag agttacaagc gcttctacaa ccccaatgat tatcatgtgt
    11701 acctttctgt tttttatacc ttcaaataat gactgcaccg ctgctgctgt caaaccaaca
    11761 gcaatcaatc cgatattaac agaactgtta ataaaatatt caagtagaaa caacataatc
    11821 ccaacaagta atgctataag cgaaccaata agactacttt tcactgattg tttattttca
    11881 aattctttgc ctttactttt ttccttttgg gcagccataa ggatatcttt atttttcatc
    11941 tgaaatctcc attccaagtt ttattggatt tatcatatcg ttccaagttt tctttgtcaa
    12001 tagaaattcc aaattttctt ggaattttat ataaaagtaa tgaggcataa ttcacattgc
    12061 tttcccttac atgctttccc ttataattgt tactaatgta tcatgagtat aatatcgctg
    12121 ttgtatatga gatacatttc ttcagcattc tctatttcgc aataactaaa taaaattgtc
    12181 attttatatt tgatgaatac tcaaacaaat agcatatctg tcacaaaatt attttataat
    12241 attgattcag tgtaaagagg tttgggaatt atgcttttaa ttcattatta agtttaaaaa
    12301 acaactatat ttttatttca cattttagtg tgtagacacc tttttgagaa ataagaggta
    12361 ctgatactat atggtaataa agcgtgatgt atacttaaat aaactgcaat cgggtcatgt
    12421 aacacctcaa attaattatg taattgcaat tacgaagagt tttgagttat ttatctttct
    12481 gcccgtttat aaatccctct taatggatat gcctcctact attattaaat caacaaatga
    12541 agaagttacc cattatcctt atttcaaact tgttttttct tttaaaaaca ctatgctgta
    12601 ctgcttaaac ccgcctgata aggcataagc tcttttaaac ccatgattaa tcaatatatt
    12661 ctgtgtggca tttcctgttg tgcctttatt gcagtaagtt acagttacaa cttccttatc
    12721 aagttgcggt atggctgctc tgaaatctgt atgggggatg tttatcgctg tccttatatg
    12781 tcccttctca tattgcttct ttactcttgt atcaatcaat tgatactttt cctctgattt
    12841 aattaatgct tccagctctt gtgcagttat aagtttcctt cctctgctta aaatattatc
    12901 aagaatcata cctgtgtaca taacaggatc ttttgtagtc gcaaaaggcg gagcgtaagc
    12961 taaatcaaga tggaataaat cctcggcttt tgctttaaat gtaatggcag tagcgaatac
    13021 atcaatcctc ttatcgactc cttcataacc tattatctgc acgcctaaaa gaagacctgt
    13081 taccttatcc gcaatccctt ttataactat ctctttaccg cctatatact ccggtttatt
    13141 tggtttaata ttatgacata ccacaacatc atatccttgg tctcttgcct ctctttcaga
    13201 tagccctgtc tgagctacag caagatcaaa tatcttgaat ataccagttc ccagtattcc
    13261 tctgaattcc aaagaacctc ctgtagcgca gtctcctgct attcttcctg tcttgtttgc
    13321 agtagagcct aaagggcgat ataaaggttt tccatttatg acgctgaatt gttctataca
    13381 gtcaccgcag gcataaatat cttctacatt cgtcctcatt tttttatcta ctttgatagc
    13441 tcctgatatt ccaagttcaa tacctgcttt ttttgcaagt gctgtatctg gccgtacacc
    13501 cgttgaaaga agaatcatat cagccaaaat cgatctacca ttagcaagca ttactgaagt
    13561 atctgttatc tcggttacca aagcatttgt tatcacctca acaccgtttg ctttcagatg
    13621 ttcttcaaca taaactgcca tatcaccatc aagacctgga gtcacctgag gcagtctttc
    13681 tgccattgtt acttcaatac ccagtttctt taaattctca caaacctcaa gacctataaa
    13741 tccggtacct acaataacag ctgtttttgg cctctttcct gctatatatt ccttaatctt
    13801 gttcatatca tttatattgc gtaaagtaaa aacattagta ttatctgccc cccttatcgg
    13861 aggaataaca gatttagcac cggttgcaat tattagcttg tcataatagt caaagaaaac
    13921 ttcttctgta aaaaggttct taacttccag cttcttctct tttggattaa tactcaaaac
    13981 ttcatgccct attaagatat ccacattgta tttgctccta aagaaataag gatcacgagg
    14041 cgtcaacgca tcagcacttt caacttctcc gccaatatag taaggcatcc cacaacccga
    14101 ataagaaatg tatgtgtcct tttcatatac tgctatttca agctcttcac tgtttcttct
    14161 tgcctttgct gcagcggagg ttcctgctgc tactgctccg attatgacta
//
LOCUS       DAOSXM010000057        14152 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2111
            NODE_2718_length_14152_cov_9.352983, whole genome shotgun sequence.
ACCESSION   DAOSXM010000057 DAOSXM010000000
VERSION     DAOSXM010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864178
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 14152)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14152)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:24:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,261
            CDSs (total)                      :: 2,229
            Genes (coding)                    :: 2,218
            CDSs (with protein)               :: 2,218
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 3 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14152
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2718_length_14152_cov_9.352983"
                     /isolate="AMR_MDS_2111"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..586)
                     /locus_tag="PLQ68_07715"
     CDS             complement(<1..586)
                     /locus_tag="PLQ68_07715"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HXK72578.1"
                     /translation="MSINSTEDTVYIVTGVSGFIGFEIADQLCKEGYATRGLIRKINP
                     ITPFPKELELIKGDILDIESLEKLYHDTEDKKTVMIHSAAYLSVKKNDPNCEHINIQG
                     TKNIIEICKKYSSKLVYISSVDTLDNSQQLVKEPDSFNPDIHFTSYARSKAIASQLVL
                     DEIQKGLDGVIILPSAVAGPNDYRKGFIYDMLSIY"
     gene            737..1249
                     /locus_tag="PLQ68_07720"
     CDS             737..1249
                     /locus_tag="PLQ68_07720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72579.1"
                     /translation="MTDSNDDYLKQMESAEVRLKMEQNPDYMDYLTGKKCWFCKQTTA
                     SDAYMYRAKDKTIKSSDVLKTEYEFKEYNVPRCQECANVHNKINGTKIWLFLSFFVLW
                     WLPFIIFPNSPAKGWISFILFVAGIVVFCIKRRILFVKYPQMINFKDSASGYYDIIKI
                     GKYLTKNSKS"
     gene            1292..2917
                     /locus_tag="PLQ68_07725"
     CDS             1292..2917
                     /locus_tag="PLQ68_07725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72580.1"
                     /translation="MEFKHKLPAMKADGRDCGNYNNPEIGYPCLLSTLNHELGDFIAT
                     LRKIVRDERRLVFIDNKVLVAYLNWIRDHVHMMKAFNHFEYDLKSFMDFILDNQRDSG
                     YFFELIKQMDDPHWQFVNPGWYKLYQSDNLAAVRLEIEADIEYLMVEGAVAVYRATGD
                     DEWIKKTLPKLEKGIEYMMNDPKRWDNERGLVKRAFTIDTWDFVYNKPGSNRKIEEDT
                     PMSIMHGDNSGVYAACVSLSWLNKRFGFDEKASYWSEKAGNLMESLNKYLWNGKFFIH
                     QLHLNHEGADNLERERLSLSNTYDINRGATNLSQADSIISEYIKRREQNNHFAEWYSI
                     DPPYERFNGLGVNKYVNGCICLFTAGELAKASFNNGYEEYGWDILCRLMKLAKKDGTL
                     YFLYSSETQDPQSGGPSGWGAAAIHSAIDEGLAGIIDTDVLYNKISFSPKWVVTPYTD
                     LRYVTGYEISKRHIDIRYMMNDKGMLYEIKSPSKQINCHILIPKDTSVKEVYVDGNMV
                     TFCKSIIRNSVYLDFTVVNSEDISVNRIECIFE"
     gene            complement(2932..4470)
                     /locus_tag="PLQ68_07730"
     CDS             complement(2932..4470)
                     /locus_tag="PLQ68_07730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72581.1"
                     /translation="MKKTLTIILIIALLSAFTYPVIAQSESTLKEENVYGILDSHGQV
                     KKIYVVNSFNSKSITDYGTYTDLKNLSTDDTINIQDDKITINSDTDKIYYQGTLPSGI
                     LPWLIDIEYYLDGEVKSAEQIASKNGKAEILVSIKPNPDSDKFFYSNYTLSVNVELDE
                     RVFFDIQTDNAVIANAGMYKQLSYTVLPGNSIDISIKADVKDFQLKPITISGVRMIMD
                     MQIDADSLTQRFTELSSAIASLNDGVGELTKAFEAYIEGLTQYTDAQNSFNEGVSQLA
                     EGISDIESGSASLAYGLNAVSLQGDPLSQAINGIVEQAFSNANAQIAQLNLELPILTE
                     QNYVSILENIPQLSILKTELDNMMQLKTGFSMYTSSVSQLSQAASQLSAGIVTLNTSA
                     KAIKQYCDTLTATAATLLDAATQLKKGLLDYKTGINTLDNETKGMAEVISKEIDSMIS
                     DIFGNSDIVRSYVSDKNTSVSSVQFVMKTEAVESQKADETVIIEEPRLTFWQRLLKLF
                     GLYE"
     gene            complement(4467..>5298)
                     /locus_tag="PLQ68_07735"
     CDS             complement(4467..>5298)
                     /locus_tag="PLQ68_07735"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HXK72582.1"
                     /translation="QTVLNDELEKLDGVKEIISYDTMISSSIPSEFVEGMDSMFNSQN
                     YSRIILYTDYETENVRTFEALDTISSTISDYYNDFYIIGESASVYDIKNVVTADNTIV
                     TVVAVLGIFLVLLVIFKSISIPMILVFTIESAIWINLAFTYFQDKPLNYIGFLIINTV
                     QLGATVDYAIFITDRYVSDRTLHLPKEAMTKTLTNNITAILISAGLLAFAGFTLTLTS
                     TNPIISELGVLLSRGTVLSFAMVSMALPALLILLDFMIRKTTFKHNFYVTKKKRGNLK
                     "
     assembly_gap    5299..5398
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5399..6369)
                     /locus_tag="PLQ68_07740"
     CDS             complement(<5399..6369)
                     /locus_tag="PLQ68_07740"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HXK72583.1"
                     /translation="MEKFTQFIVKNNKIIILIFLIITVICGILAVGVKVNYNLEDYLP
                     KDSNSTIGLNIMKEEFDEDFPSANVMLDDVSIQQALACKEKLKNIPHVTSVTWLDDVV
                     GIDVLLSTPFEYIDNTTTEAYYKDNNALISLAIEPGYELDTVNELHKIIGEENALSGS
                     SVNKAVLQEMSSSETLKAMLILVPLIILILLLTTSSYIEPVIYLVSIGIAILINMGTN
                     IFFSDISFITKTVSPILQLAVSLDYAIFLMHSFKEYRTDHEPKEAMKMAIKKSISSIS
                     GSAATTIIGFLALIFMRFGIGMDLGLNLVKGVALSFISIMIFMPALT"
     gene            6532..7101
                     /locus_tag="PLQ68_07745"
     CDS             6532..7101
                     /locus_tag="PLQ68_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012958755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR-like C-terminal domain-containing protein"
                     /protein_id="HXK72584.1"
                     /translation="MKVKKEDRRVKYTKMVLKKSLINLLSKKDISSITIKQICEDADI
                     NRATFYTHYTDQFDLLRKIENEFLENVKVYISQFKLRKADAALVEMMEKIFEYIKDNA
                     DLCKLLLSKQGNLEFQKRIMMLIYDSNIIVKPGESLKKGEEEYMYAFIITGCVGVIHN
                     WLDEGMKQSSRMMANTIFNVVRSLPITFE"
     gene            7200..9431
                     /locus_tag="PLQ68_07750"
     CDS             7200..9431
                     /locus_tag="PLQ68_07750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72585.1"
                     /translation="MRKYTKELLAILIFLVSTVIAITVITNITMPKRYSYGSTWGSYL
                     KEPKNSIDVLFFGSSLVYCNIVPSVIYEHSGITSYVMAGPEQTIPLSYYYIKEAVKSQ
                     NPKTIMLDASGMFFNKYESYTKVNIGYMPWSLNRLSAIENAAEQEQQTGLYFPLYNYH
                     TRWSSLTLGDIKTGVFGYERDIYAGYTFLSKIEPQGSPWPRNEKFDIQTYKENIEYMK
                     DIAAFCEKKDISLIVFISPSFSALKQDNLDILKKDIGKIKNISYIDFNERFDDFDINV
                     KTDFFDLMHFNALGAEKFSIYLSDMLKSLDLEATDNEDMSLWWSRISNFKKQKALIDI
                     PKQTDIESTEEPDQNVLDEPIEVIETEVTDYNLIEYENMISLMKNSDYEEAIQIYEQS
                     ENLKKGYEKASLYYAYAKALKYHTMLNNEEQVELLKSHINTDFILTIQYKPVHPYKRT
                     AYGATWGNYFKEPKNSIDVMFFGSSLIYCDIAPPAIYDKAKITSYVMGGPEQTMPITY
                     YYIKEALRTQSPKVIFVEVTGLLYDKYYSNTKTNILYMPKSINRLSATFEGANPDTWD
                     ELLFSLYDYDDDTITDISEYSSYGMDNMAGYTYLDNIDIVGDPVYRSISYNEEEYKLN
                     LSYLHKISKLCEEEKIQLEYFIAPSYYRIPSEYMDIIEKDMSNMEYGRYTDHNIIYPD
                     ERIDRTTHFYDILHFNCYGAKQYSEFLAELLVSEYKLSESLYEDTTLWQSRVDNFMYM
                     LSN"
     gene            complement(9464..10192)
                     /locus_tag="PLQ68_07755"
     CDS             complement(9464..10192)
                     /locus_tag="PLQ68_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006355134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HXK72586.1"
                     /translation="MNEDKRFAVLIDADNVSDKYIKYIFDELAKSGTITYKRIYGDWT
                     RPDLGSWKKVLLEYSITPIQQYGYTTGKNATDSAMIIDAMDILYKDNVEGFCIVSSDS
                     DFTRLVSRLREAGKYVVGMGEKKTPSPFIASCNQFKYLEILGQDEIQGKQKDMTDSIT
                     PLENVIKAIEVIAEDISDDDGWSPLSDIGNILSKRFSDFDVRNYGFSKLTPLVSSLKK
                     FDVKSIKKNNIKLIYIKKRQNDNK"
     gene            10662..11672
                     /locus_tag="PLQ68_07760"
     CDS             10662..11672
                     /locus_tag="PLQ68_07760"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HXK72587.1"
                     /translation="MQQENTITSGKDRLLEILSLLYEKSDENHQLSTNDILNHLQNKG
                     MLTNRKTVKNDIEILNKHGIDIVITHGLSNTYFMGDRIFQMPEIKMLMDSVMSSRAIT
                     EHKSEVLIDKLKSLVSVHQAAELSESMCFKSAIKSSNENIYYIVDTIHNAIRLNKKIQ
                     FRKYDYTVLKEQTYRDNGKLYVVSPYELVWFEDRYYLIGFFEDSGLVSSTRVDNIEMP
                     TILIEDSHAIPSWFDMGEYVLSMFKMYAGPMSEVELICENSMMRIILDRFGKNVETSV
                     INEEHFKVKVTVSISPTFYGWVFQFGGMIRISSPKTVCDEYLNMIEQNLKFITADKGR
                     KQ"
     gene            11673..11867
                     /locus_tag="PLQ68_07765"
     CDS             11673..11867
                     /locus_tag="PLQ68_07765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72588.1"
                     /translation="MDIIKEDIIRVLGSKKALTIDELFSCDYLAYADRSSIYFALESL
                     TTQGLIQKRYIKPDMPVYCV"
     gene            complement(12029..13969)
                     /locus_tag="PLQ68_07770"
     CDS             complement(12029..13969)
                     /locus_tag="PLQ68_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011026232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HXK72589.1"
                     /translation="MTNVKFKKVLEPGKIGNLELKNRFVMAPMVTNYCDYDGCVTERF
                     KAYHEARAKGGVGMIIIEATYVTPSGRGFPNEVGISKESHIKGLRELTDTVHKYGTKI
                     ATQLYHGGRQTHAAVTGQTPVAPSSIACPVCQEVPEELTVEEIKEIVEAFGQGARRAK
                     EAGFDAIELHGAHGYLLNQFLSPYSNKRTDEYGGSFENRMRMPLEILKATRESVGADF
                     PIIYRLTSEEFVEGGLTIEDTKMFSRILVENGIDAINVSGGVYETAAMIIQPAAVPQG
                     LFVENAAAIRKAIDAKVPVMVVGRIKEPEMAETILENGQADFIVMGRGLLADPEFVNK
                     IASNDLDGIRRCIACNQGCIDRLFMNLDIGCLVNATTGNEMDYDMDKKTTKKKVVVVG
                     SGPAGLEAARVAALRGHDVTVYEKDDKIGGQLNIATVPPHKDEVMDLIKFLTESLHRL
                     NVKIVTNKEADVETIKDESADAVIVATGSTPVTPNMSGVDRKEVVNAHDVLSGKAKTG
                     KNVVVVGGGSVGCETAEYLVTQGKTVTVVEMLEDVMMDSGAAVKALMLLRLAQANVNI
                     MRKSKVIQIKDNCVLVKTENGNECVINMDTVVIAVGVKPCMTLSDKLKGARIPHVTIG
                     DCVKPRKIYEAIQEGFAAGYNI"
BASE COUNT         4679 a         2296 c         2431 g         4646 t
ORIGIN      
        1 gatatattga aagcatatca tagatgaatc cttttctgta atcattaggt ccggcaacag
       61 ctgatggaag aataatgact ccatcaagtc ctttttggat ttcatcaagt actagttgcg
      121 aagctatagc tttagagcgt gcatatgatg tgaaatgaat atcagggtta aaagaatctg
      181 gctctttgac cagctgttgt gaattatcca gtgtatcaac acttgaaata tatacaagct
      241 tcgatgaata ctttttacaa atctcaatta tattctttgt accctggata ttaatatgtt
      301 cacagttagg gtcattcttt ttaactgaca gatatgcagc agaatgaatc attacagttt
      361 ttttatcttc tgtatcatgg taaagttttt caagcgattc tatatccagt atatctccct
      421 ttataagttc aagctctttt ggaaaaggcg ttattggatt aatctttcgt attaatcctc
      481 ttgttgcata tccttctttg cacaactggt cagcgatttc aaaccctata aaaccgctta
      541 cacctgtaac aatatataca gtgtcttcag tactatttat actcatacca taccccttgg
      601 tctatgactc tttctagatg attatatcat cagatatagc aagttatcag cattttattg
      661 tcatatccca agataagtta tataatttta taaatatttc tatggaaata ccgttttttt
      721 atttctggag gagctaatga ctgatagcaa tgatgattat ttaaaacaaa tggaatcagc
      781 tgaagtaagg ttgaaaatgg aacagaatcc agattatatg gattatctta caggtaaaaa
      841 atgctggttc tgtaaacaaa caactgcatc agatgcatat atgtacagag caaaggataa
      901 gactattaaa tcatctgatg ttttaaaaac tgagtatgaa ttcaaagagt acaatgtccc
      961 aaggtgtcaa gaatgtgcaa atgtacataa caaaataaat ggaacaaaga tatggctgtt
     1021 tctttcattc tttgtattat ggtggcttcc atttattata tttccaaatt cacctgcaaa
     1081 aggatggata tctttcatac tctttgttgc gggcatagtg gtattttgca ttaaacgaag
     1141 aatattattt gttaagtatc ctcagatgat taactttaaa gacagtgctt caggatatta
     1201 tgatattata aaaataggga agtatcttac caaaaatagt aaaagctaga tactaaataa
     1261 ctgatagtaa taaatattga ggatgaatag tatggaattc aaacataaat tgcctgctat
     1321 gaaggcagac ggaagagatt gcggtaatta caataacccg gaaattgggt atccctgcct
     1381 tcttagtact ctaaatcacg aattagggga ctttatagcc acacttagaa agatagtgcg
     1441 agacgagaga aggcttgtat ttattgataa caaggtgctt gtcgcttatc tgaactggat
     1501 aagagatcat gttcatatga tgaaagcatt taaccatttt gaatatgact taaaaagttt
     1561 catggacttc atattagata atcagcggga ttcaggatac ttttttgagc ttataaaaca
     1621 gatggatgat ccgcactggc agtttgtaaa tcccggctgg tataagctat atcaatcaga
     1681 taatttagct gctgtaagat tggagataga agcagatata gaatacttaa tggtagaagg
     1741 ggcagtagcg gtatataggg ctacagggga tgatgaatgg ataaaaaaaa cattacctaa
     1801 gcttgaaaag ggaatagagt atatgatgaa tgatcccaaa agatgggata atgaaagagg
     1861 acttgtaaaa agagccttta caatagatac ttgggatttt gtatataaca agcctggttc
     1921 caacagaaag atagaagaag atactccgat gagcataatg catggcgata actccggagt
     1981 atatgctgca tgtgtgtcat tatcatggct gaacaaaaga ttcggttttg atgaaaaagc
     2041 ctcttattgg tctgaaaagg ctggtaatct aatggagtct ttaaataaat atctttggaa
     2101 tggaaagttt tttatccatc agttacactt gaatcatgaa ggtgctgata atcttgaaag
     2161 agaaagatta tctttatcca atacatatga tataaacagg ggagcaacta atcttagtca
     2221 ggcagatagt ataatatctg aatacataaa aagaagagag cagaataatc attttgctga
     2281 atggtattca atagatcctc catatgaaag atttaacgga ttaggagtta ataaatatgt
     2341 aaacggatgt atttgtcttt ttacagcagg cgagcttgct aaagcatcat ttaataacgg
     2401 atatgaagaa tatggctggg atatattatg caggttaatg aaactggcga aaaaggatgg
     2461 tacattgtac tttttgtatt catctgaaac acaagatcct caatcaggtg gaccttcagg
     2521 ttggggagca gcagctatac acagcgcaat agatgaaggc ttggcaggta tcatagatac
     2581 tgatgtcttg tataacaaaa tatcattttc tccaaaatgg gtagtgacac cttatacgga
     2641 cttaagatat gtgacaggtt acgagataag taaaaggcat attgatattc gatatatgat
     2701 gaatgataaa ggcatgttat atgagattaa atcaccatct aagcagatta actgtcatat
     2761 ccttattcca aaagacactt ctgtaaaaga agtatatgta gatgggaata tggttacttt
     2821 ttgtaagtca atcattagaa actcagtata tcttgatttt actgttgtca attcagaaga
     2881 tataagtgtg aacaggatag agtgtatttt tgaatagttc actaatttta cttattcata
     2941 cagcccaaac agttttaaca gcctttgcca gaaagtaagc ctgggctctt ctattatgac
     3001 agtttcgtct gctttttgtg attcaacagc ttctgtcttc ataacaaact gcacgcttga
     3061 tacagatgta ttcttgtctg atacatatga ccttacaata tcagaattac caaaaatatc
     3121 acttatcata ctgtcaattt ctttgcttat tacttctgcc ataccttttg tttcattatc
     3181 aagagtattt attcccgttt tgtaatctaa aagacctttc tttagctgtg ttgctgcatc
     3241 taagagggta gcagcagttg ctgttaatgt atcacaatac tgcttaatgg cttttgcaga
     3301 tgtattaagc gtgactattc ctgcgcttaa ctgtgatgca gcctggctta actgtgatac
     3361 tgatgatgtg tacattgaaa aacctgtctt tagctgcatc atattatcaa gttcagtctt
     3421 taaaatactc aactgtggaa tattttctaa tatggataca taattttgtt cagttaagat
     3481 aggaagttca agattaagct gagctatttg agcatttgca ttagaaaaag cctgctcaac
     3541 aataccatta atggcttggc ttaagggatc gccttgtaag cttacagcat ttaagccgta
     3601 agctaaagaa gctgaacctg actctatgtc agatatacct tcggctaact gactgactcc
     3661 ctcattaaaa ctgttctgag catctgtgta ctgagtcagc ccttctatat atgcttcaaa
     3721 tgcttttgta agttctccta cgccatcatt caagcttgct atagcactgc ttagttctgt
     3781 aaacctttgc gttaatgagt ctgcatctat ttgcatatcc ataatcattc ttacaccgct
     3841 tatagttatc ggttttagct ggaaatcttt gacatcagcc ttaatggaaa tatctatact
     3901 gttaccagga agaaccgtat aacttaactg cttgtacatc cctgcatttg ctataactgc
     3961 attatcagtc tgaatatcaa aaaacactct ttcgtccagt tctacattta cagataaagt
     4021 atagttactg taaaagaatt tgtctgaatc gggattaggt ttaatggata caagaatttc
     4081 tgcttttccg tttttcgatg ctatctgttc tgcgctttta acttcaccat caagataata
     4141 ctcaatatca attaaccatg gaagaatgcc agagggaagt gttccctgat aatatatttt
     4201 atctgtatca gagttaatgg tgattttgtc atcttgaata ttaattgtat cgtcagtaga
     4261 gagattcttc aaatcagtgt atgtgccata atctgttata ctttttgaat taaaggaatt
     4321 aacaacatat atcttcttaa cttgtccatg tgaatctagt ataccataca cattttcctc
     4381 tttcagtgtc gactctgatt gagctataac aggatatgtg aatgctgata aaagagctat
     4441 tatcagtata atggttagtg tctttttcat tttaaattac ctcttttttt ctttgttacg
     4501 taaaaattat gcttaaatgt tgtttttcga atcataaaat ctaaaagtat tagtaaggct
     4561 ggcagcgcca tggataccat tgcaaaagac aatacagttc ctcttgataa aagcacgccc
     4621 agttcggaaa ttattggatt agtagatgtc agcgttaaag taaatcctgc aaatgccaac
     4681 aggcctgcag atataagtat cgcagttata ttattggtta atgttttggt catagcttcc
     4741 tttggcagat gtaaagtgcg gtcagacaca tatctgtctg taatgaatat agcgtaatcc
     4801 acagtagctc cgagctgtac tgtgtttatg attagaaagc ctatgtagtt taaaggcttg
     4861 tcctggaaat atgtaaaagc aagattgatc catatagctg attctatagt gaataccaaa
     4921 atcataggaa tggatattga tttgaatatt acaagcagta ccaagaatat gcccagtacc
     4981 gctacaacag ttactattgt attatctgct gtgacgacat ttttaatatc atacacgctg
     5041 gcgctttcgc caatgatata gaagtcgtta tagtaatctg aaatggtact agaaatggta
     5101 tctaaagctt caaaagtcct tacgttttca gtctcgtaat cagtatagag tatgatacga
     5161 gagtaatttt gtgaattgaa catgctatcc attccttcaa cgaattctga aggtatagag
     5221 gaacttatca tagtgtcata tgaaattatc tcttttactc catcaagttt ttcaagttca
     5281 tcattcaaaa ctgtctgcnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnat
     5401 tgttaaagca ggcatgaata tcattatgct tatgaagctt aatgcaactc cttttacaag
     5461 attaaggcct aaatccattc caataccgaa tctcataaat atcagggcga gaaaaccgat
     5521 aatagtagtt gctgcacttc ctgaaataga tgaaatcgac ttttttatag ccattttcat
     5581 tgcttcttta ggttcatgat ctgtcctgta ttctttaaag ctgtgcatta agaatatagc
     5641 atagtctaaa gatactgcta actgaagtat cggacttaca gttttggtta taaaggaaat
     5701 atcactaaag aatatgtttg ttcccatatt tatcagtatt gctataccga ttgaaacaag
     5761 atatattaca ggttctatat atgaagaagt ggtgagaaga agtattaaga ttataagagg
     5821 gactagaata agcatggctt taagcgtttc agatgaggac atttcctgta agacagcttt
     5881 gtttactgaa gagcctgata acgcgttttc ttcccctatt atcttatgca gttcatttac
     5941 ggtatccagt tcatatcccg gttctatggc taatgatatg agcgcattat tatctttgta
     6001 ataagcttct gttgtggtgt tgtctatata ttcaaaaggt gttgataata gaacatctat
     6061 acctactaca tcatcaagcc atgtgacaga tgtgacatga ggaatgtttt taagtttttc
     6121 cttacatgca agcgcttgct gtattgatac atcatcaagc atcacatttg cagaagggaa
     6181 gtcttcatca aactcttctt tcattatatt cagccctatg gtggaattgc tgtcttttgg
     6241 aagataatcc tctaggttgt aattgacctt gacgccaaca gccagtatgc cgcatataac
     6301 agtaatgata agaaatatca atatgattat tttattgttt ttaactatga actgtgtaaa
     6361 tttctccatc atttatcctt tacattaatc aacacaatgt tgacaaacta tcatgagtat
     6421 aatatattat caccatatat ttaagtcaac acacagataa ttaataaatg tatgttgata
     6481 aacatatata ctcagttatg tatattatcc gacatattaa gaggagttgt catgaaagtg
     6541 aaaaaagaag acagaagggt taaatatacc aaaatggtat tgaaaaaaag cctcataaat
     6601 cttttatcaa aaaaagatat ttcatctata acaataaagc agatttgtga agatgcagac
     6661 atcaacagag ccacatttta cacccattat accgatcagt tcgatctttt acggaagatt
     6721 gaaaatgaat ttttagagaa tgtaaaagta tatatatctc agttcaagct aagaaaagca
     6781 gatgctgctt tagttgagat gatggaaaaa atatttgagt atataaaaga caatgctgat
     6841 ttatgtaaac ttcttctttc caagcaggga aacctggaat ttcaaaaaag gattatgatg
     6901 ctcatttatg acagtaacat aatcgttaaa cctggggaaa gcttaaaaaa aggagaagag
     6961 gaatatatgt atgcatttat tatcacagga tgtgtaggag ttatacataa ctggctagat
     7021 gaaggaatga agcagtcatc taggatgatg gcaaacacaa ttttcaatgt agttagaagt
     7081 ctgccaataa cttttgaata ataaaacaat caaaacaata tattaaagtt ataaaaaata
     7141 caatacagat aaaatagatg gtatataata atataagtgc aaactgcatt tgaaaaatta
     7201 tgagaaaata tacaaaagaa ctattagcga ttttaatatt cctagtaagc acagtaatag
     7261 ctattactgt tattactaat ataacaatgc ccaaaagata tagttatggt tcaacatggg
     7321 gtagttatct taaagagcct aagaattcta ttgatgtact gttctttgga agttctttgg
     7381 tttactgcaa tatagtgcct tcagtaatat atgaacattc aggtattact tcatatgtaa
     7441 tggcaggacc tgagcagaca atacccttgt catattacta cattaaagaa gcagtaaagt
     7501 ctcaaaaccc caaaaccatt atgctggatg caagcggaat gttcttcaat aaatatgaaa
     7561 gttatacaaa agtaaatata ggatacatgc catggtcttt aaacagattg tcagctatag
     7621 aaaatgctgc agagcaagaa cagcagaccg gcttatactt ccctctatac aattaccata
     7681 caagatggtc ttcattaacg ttaggggata taaaaaccgg tgtttttgga tacgaaaggg
     7741 atatttatgc tggatataca ttcttaagta aaattgagcc tcagggcagt ccatggccga
     7801 ggaatgaaaa gttcgatata cagacatata aagaaaatat tgagtatatg aaggatatcg
     7861 ctgctttttg tgaaaaaaaa gacatatcat taattgtctt catcagtcca agcttctcag
     7921 cactgaaaca ggataatctt gatatactta agaaggatat aggtaaaata aagaatataa
     7981 gttatataga ttttaacgaa agatttgatg attttgatat aaatgttaaa acggactttt
     8041 ttgatcttat gcatttcaac gcattgggag ctgaaaaatt ctctatatat ttatcagata
     8101 tgctaaaatc ccttgattta gaagctactg acaatgaaga tatgtcatta tggtggtcaa
     8161 gaatatcaaa cttcaaaaaa caaaaagcac tgattgatat tccaaagcaa acagatatag
     8221 aatctacaga agaacctgac cagaatgtgc tagacgaacc aatagaagta atagaaacag
     8281 aagtaacaga ttataactta attgaatatg aaaatatgat aagtcttatg aaaaactcag
     8341 attatgaaga agcaatacag atttatgaac aatcagaaaa tctgaaaaaa ggttatgaaa
     8401 aagcaagtct ttattacgca tatgcaaaag ctcttaaata tcacacgatg ctgaataatg
     8461 aagagcaggt tgaactttta aaatcacata taaatactga tttcatactc actatacaat
     8521 acaagcctgt tcatccatat aagaggacgg catatggcgc tacatgggga aattatttta
     8581 aagaacctaa aaactctatt gatgttatgt tctttggaag ctcattgata tattgcgata
     8641 tagctccgcc agctatatat gataaagcga aaataacatc atatgtaatg ggaggaccgg
     8701 aacagacaat gccaattaca tactactata taaaagaagc attaaggacg caaagcccta
     8761 aagtgatatt cgttgaagta acaggtcttt tgtatgataa atattactct aatacaaaaa
     8821 ccaatatact ttacatgcca aaaagcatta acaggctgag cgcaactttt gaaggcgcta
     8881 atcctgacac atgggatgaa cttctatttt cgttatatga ttacgatgat gatacgataa
     8941 cagatatctc agaatattca tcatatggaa tggataatat ggcaggatat acatacttag
     9001 ataatattga tatagtaggc gatccggttt acagaagtat aagttataac gaagaagagt
     9061 ataagttgaa tttatcttat cttcataaaa tatctaagtt gtgtgaagaa gaaaaaatac
     9121 agttggaata ttttatagct ccttcatact acaggattcc atcagaatat atggatataa
     9181 tagaaaaaga catgagcaat atggaatatg gaagatatac tgaccataac attatctatc
     9241 ctgatgaaag aatagacaga accactcatt tttatgacat actccatttt aattgttatg
     9301 gtgctaaaca gtattctgaa tttctagcag aattgctagt aagtgaatac aaattatccg
     9361 aatcattata tgaagataca accctttggc agtcaagggt tgataacttt atgtatatgc
     9421 tatctaatta attacttaat tatttaatta cctaattatc catctattta ttatcgtttt
     9481 gtctcttttt tatgtatatg agttttatat tattcttttt aatcgatttg acatcaaatt
     9541 tcttaagtga tgaaaccaaa ggagtaagtt tgctgaatcc atagtttctc acatcaaagt
     9601 cagaaaatct tttagagagg atattgccta tatcactaag agggctccat ccgtcatcat
     9661 cagatatatc ttcagcaatt acctcaatag ctttaattac attttccaaa ggagttatgg
     9721 agtcagtcat gtctttttgc tttccctgta tctcatcctg acctagtatc tcaagatatt
     9781 tgaactggtt gcacgatgca ataaacgggg aaggagtctt tttttctccc atacctacta
     9841 catacttacc agcttctcta agtcttgata ccagtcttgt aaaatcggaa tcagaggata
     9901 ctatgcagaa tccttcaaca ttatccttat acaaaatatc cattgcatct ataatcatag
     9961 cggaatcagt agcatttttt cctgttgtat acccgtactg ctgtataggc gttattgagt
    10021 attccagcaa taccttcttc catgagccaa gatcaggtct tgtccagtcg ccatatattc
    10081 ttttgtatgt tattgtgcct gattttgcca gctcgtcaaa aatatatttt atatacttgt
    10141 cagatacatt atccgcatct attaaaacag caaatctttt gtcttcattc atgtgttata
    10201 cctcatatat atatgataac ataaatcaat aaaattttct ctatatgtta aattgcaaaa
    10261 aagcagacac aataaagagt ccaaaagact aacatttttg tcataatagc agcacatggc
    10321 ctggaatcag gatatcttat acataattgt ccatatgaac atatatttat attatgttgt
    10381 tataaataag tatacttatt gcttgcaaat cattaaatca taggaatact tacatgaaaa
    10441 tgtaagaaat atatgttaat tatttgagtg cttaaataga cactgtcctg atatcaggac
    10501 agttaaacta actttactat taatttgcta agatttattg actgcgtaat ttaatagtgt
    10561 atattagtat tatatattag tatcattttg gggattatat caagcttttt gtgggagctg
    10621 gtattgggat ttgattcttg tagataaaga agaggggttg catgcagcag gaaaatacaa
    10681 taacaagcgg taaggacagg cttttagaaa ttcttagtct tctttatgaa aagtctgatg
    10741 aaaatcatca gctttcaact aatgatatct taaatcatct gcagaataag ggtatgctga
    10801 caaacagaaa aactgtgaaa aatgatatag aaatattgaa taaacatggt attgatatag
    10861 ttattactca tggactcagt aatacatatt ttatggggga tagaatattt caaatgccgg
    10921 aaattaagat gctcatggac tctgttatgt catcaagggc aataacagaa cataaaagtg
    10981 aagtattaat agataaactt aagagtttgg tgtctgttca tcaggcagcg gaactatcag
    11041 aaagcatgtg ttttaaaagc gctattaaga gcagtaatga aaacatatat tacatagtag
    11101 atacaataca taatgcaata aggcttaata agaaaataca gtttagaaag tatgactata
    11161 ctgtattaaa agaacaaaca tatcgtgata acggtaaatt gtatgtagta agtccttatg
    11221 aactggtttg gtttgaggac agatattatc tcataggctt ttttgaagac tctgggttag
    11281 tatcaagtac aagagtagat aatattgaga tgcctactat attgatagaa gactctcatg
    11341 ctattccttc atggtttgat atgggtgaat atgtattgtc aatgtttaaa atgtatgcag
    11401 gtcctatgtc agaagttgaa cttatatgtg aaaacagcat gatgaggata atactggaca
    11461 gattcggtaa aaatgtcgaa acatcagtta tcaatgaaga acactttaaa gtaaaagtaa
    11521 cagtatcgat aagtccgact ttttatggat gggtatttca gtttggaggt atgataagga
    11581 tatcatcacc caaaacagtt tgtgatgaat atcttaatat gatagaacag aacttaaaat
    11641 ttattacagc agataaaggg aggaagcagt aggtggacat tataaaagaa gatattataa
    11701 gggtactggg ttcgaaaaaa gcgcttacaa ttgatgagct gttctcttgt gattatcttg
    11761 catatgcaga cagatcatca atatattttg ctttggaatc attaactaca caaggactta
    11821 tacaaaaaag atatattaaa ccggatatgc ctgtgtactg tgtttaataa agagattagt
    11881 tatatttgat taaaaaacat aaaaaagacc ctagtaattg ggtctttttt ttatttatat
    11941 atttatttat cctgttatct gttgtctgtc ttcaatcttt ttgattacct taagacatag
    12001 acatttatgt taatatacaa tgtctgttct atatattgta accagctgcg aaaccttcct
    12061 gaattgcttc atatatcttt ctgggtttaa cgcagtcacc tattgtaaca tgtggaattc
    12121 ttgcgccttt tagtttatca gataatgtca tgcatggttt tacgcctaca gcaataacta
    12181 cagtatccat gtttataacg cattcatttc cattttcggt ttttaccagt acgcagttat
    12241 ccttaatctg tataactttg gactttctca ttatatttac atttgcctga gcaagtctta
    12301 acagcattaa agcttttact gcagcaccgg aatccatcat tacatcttct agcatctcaa
    12361 caacagtaac agtcttgccc tgagttacta gatattcagc tgtctcgcaa ccaaccgatc
    12421 ctccgcctac tacgacgaca ttcttacctg tttttgcttt accgcttaat acatcatgag
    12481 catttacgac ttcttttctg tctactcctg acatattagg tgtaacaggc gtggagccgg
    12541 tagctactat aacagcatca gctgactcat cttttatagt ttcaacatca gcttctttgt
    12601 ttgtaactat ttttacattt aatctgtgca gcgattctgt taggaattta ataagatcca
    12661 ttacctcatc tttgtgtgga ggaacagtag caatattgag ttgtccgcca atcttgtcat
    12721 ctttttcata aacagttaca tcatgtcctc tcagtgccgc tactcttgca gcttccaatc
    12781 ctgcaggacc tgaacctacg actacgactt tcttctttgt tgtcttctta tccatgtcgt
    12841 aatccatctc gttacctgta gtagcattta ccaagcaccc tatatccaga ttcatgaaca
    12901 gtctgtctat acagccctgg ttacaagcaa tacatcttct tattccgtca aggtcattgc
    12961 ttgctatctt gtttacaaat tcaggatctg ctaataagcc tcttcccata acaatgaaat
    13021 cagcctgtcc gttttccaaa attgtttcag ccatttcagg ctctttaatt cttccaacta
    13081 ccataacagg tacttttgca tcaatggctt ttcttatagc tgcggcattt tcaacaaata
    13141 agccctgcgg tactgctgca ggctggataa tcattgcagc agtttcgtaa actcctccag
    13201 atacgtttat tgcatctatt ccgttttcta ctaatatcct agaaaacatc tttgtatctt
    13261 ctatcgtcag tccgccttca acaaattctt ctgatgtaag acgatatatg atagggaagt
    13321 cagcaccaac gctttctctg gttgctttta atatttcaag gggcattctc atacggttct
    13381 caaaagatcc gccatactca tctgttcttt tgttgctgta aggcgataag aactgattta
    13441 aaagataacc atgagctcca tgtagctcaa tagcatcaaa accagcttct ttggctcttc
    13501 ttgcaccttg accgaaagct tctacaattt cttttatttc ttctactgta agttcttcgg
    13561 gaacttcctg acagactgga catgctattg atgaaggagc aacaggagtc tgtcctgtaa
    13621 ctgctgcatg agtctgtctt cctccgtgat aaagctgtgt agctatcttt gtgccatatt
    13681 tatgcactgt atctgtaagt tctcttaatc ccttaatgtg ggactcttta gatataccga
    13741 cttcattagg aaatcctctt cctgaggggg taacataggt ggcttcgatt ataatcatac
    13801 ctactccgcc ttttgctctt gcttcgtgat atgctttaaa tctttcggtt acacatccat
    13861 cataatcaca ataatttgta accattggag ccattacaaa gcggttcttt agctcaagat
    13921 taccgatctt cccaggttcc agtacttttt taaattttac atttgtcatg ttgtacctcc
    13981 ttctacttga tatgttgtac taatataatt ctacagtaaa taataggtag tgtcaaaaat
    14041 atttatcaca aaaatttaaa taacataata tgtataaaac ataaataaat taactattta
    14101 tctgtagtga cgaatttcaa gtactaaata agtttgaatt ttacatttgt ct
//
LOCUS       DAOSXM010000058        13802 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2111
            NODE_2806_length_13802_cov_9.650033, whole genome shotgun sequence.
ACCESSION   DAOSXM010000058 DAOSXM010000000
VERSION     DAOSXM010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864178
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 13802)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13802)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:24:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,261
            CDSs (total)                      :: 2,229
            Genes (coding)                    :: 2,218
            CDSs (with protein)               :: 2,218
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 3 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13802
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2806_length_13802_cov_9.650033"
                     /isolate="AMR_MDS_2111"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            101..538
                     /locus_tag="PLQ68_07775"
     CDS             101..538
                     /locus_tag="PLQ68_07775"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013869222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HXK72590.1"
                     /translation="MAIVKFSVNAKSENPTKTVVETRGFKMIVDEPANLGGTDHGANP
                     VEYVLAALSGCLNVVGHLVAKELGFTLRGLNINIEGDLDPAKFTGAKTNERAGYKAIR
                     VNLKPDTDADEATLKTWLEVVEQRCPVSDNLMNTTPVTISVNK"
     gene            complement(654..>3323)
                     /gene="carB"
                     /locus_tag="PLQ68_07780"
     CDS             complement(654..>3323)
                     /gene="carB"
                     /locus_tag="PLQ68_07780"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020492164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HXK72591.1"
                     /translation="DRWFLAKIKNLADFEKKLENEYTNDLYLEAKKLGFTDKAIKRIS
                     KTQNIPSLDATYKMVDTCAAEFEAKTPYFYSSYDKYCEARAMKKSEKERIIVLGSGPI
                     RIGQGIEFDYSSVHCVWALKNMGYEVIIINNNPETVSTDYNTADRLYFEPLTEEDVMN
                     VIKVEKPLGVVVAFGGQTAIKLTKFLDSNGILILGTSAEGIDMAEDRERFDELLSRFN
                     IIRPKGLAVMSTKQAITAAETLGYPVLLRPSYVIGGQNMVIAYTKDEIQQYMNVILKS
                     KIENPVLVDKYMMGTELEVDVISDGEDILIPGVMEHIERAGIHSGDSIAVYPHYTLND
                     ILLNKLIDCSEKLALSLGTKGLVNIQYLIYENDVYVIEVNPRASRTVPYISKVTGIPM
                     VELASKVMLGQKLKDLGFGTGLYRQPPYFAVKVPVFSFEKLTDANSALGPQMKSTGEV
                     LGIGKTLNEALFKGLTSAGFKVDADKSKGVLISVDKKDRLEILKVAKKFNELNIKIYA
                     TNSTAKAISSLGIDVQFVNKLSYDDTLIKLLDAGKIKYIVCTIPNTRGAMEDFIRLHK
                     RAIQLSVPCLTSLDTANALADILASRFNQNNTELVDINNLRSERLKLNFVKMQGSGND
                     YIYFDNSDKRINCPESLAISLCNRNFGIGSDGLILIEQSKKADALMRIFNKDGSEGKM
                     AGNSIRCVAKYLYDNNIVKKTDMRIETASGIKNLKLFTINDKVSSVTVEMGKAELDKD
                     KIPVICDTPTVIDKPVIIDDKEYNITCVSIGNPHCVVFTDRVDSVDVENIGPKFENAP
                     IFPERINTEFIRVVNKNTIKMRVWERGNGETLACGTGACAAVVAAVENGFCEKNTDIT
                     VNLRGGDLIVNYSDEGITLTGNAVLICEGTVSI"
     assembly_gap    3324..3423
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3424..4511)
                     /gene="carB"
                     /locus_tag="PLQ68_07785"
     CDS             complement(<3424..4511)
                     /gene="carB"
                     /locus_tag="PLQ68_07785"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005354269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HXK72592.1"
                     /translation="MPRDNSIKKTLIIGSGPIIIGQAAEFDYAGAQGCKVLKSEGIDV
                     VLVNSNPATIMTDKALADEIYLEPLTQETIKRIIKKEKPDSMIAGLGGQTGLTIAHQL
                     AKSGFLQKEGVRLLGTNVEAIDKAEDRQLFRDTMMSINQPCIPSDIAVDVRKAKLIAD
                     SIGYPVIIRPAFTLGGTGGGVAYNEEELLATAKSGLDASPIDQILVEKYIYGWKEIEF
                     ETMRDKNGNVIAICSMENLDPVGVHTGDSIVVAPALTLSDKEYQMLRTAALNIITELK
                     IIGGCNVQFALDPNSFDYAVIEVNPRVSRSSALASKATGYPIAKVTAQLALGYTLDEL
                     LNDITGKTCACFEPAIDYVVVKFPKWPF"
     gene            complement(4498..5574)
                     /locus_tag="PLQ68_07790"
     CDS             complement(4498..5574)
                     /locus_tag="PLQ68_07790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004033420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HXK72593.1"
                     /translation="MKERLIYLILEDNQVFEGKSFGAEIESIGELVFTTGMGGYIETL
                     TDPSYYGQICIQTFPLIGNYGIIEEDFESPRCHMKGYIVRQICDFPSNFRCQYDLNTY
                     LKNQNIPGIYDIDTRHLTTIIREHGVMNAMISHNRPTKQMIEKIKSYKIFDSVASVSS
                     KKIEESYIPIPKFNVLLIDYGSKKNIIKELNKRYCNVTSLPHDAMVEEILDYKADGVM
                     LSNGPGNPKDNIEAIQTIKKIVGKVPVFGICLGHQLLALAMGHDTVKLKYGHRGANQP
                     VIDKKTNSVYITTQNHGYSVVAKESDMSFVNANDFTCEGMEYPDHLAFSSQFHPEACS
                     GPKDASFLFDKFIKMMEDFRNAKR"
     gene            complement(5571..6485)
                     /gene="argF"
                     /locus_tag="PLQ68_07795"
     CDS             complement(5571..6485)
                     /gene="argF"
                     /locus_tag="PLQ68_07795"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002852373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HXK72594.1"
                     /translation="MKHLLKLIDLDDDDITDILNLADQLKYEKKNEIGKKYLEGKKLG
                     MIFEKSSTRTRVSFEAGMYELGGHALFLSANDLQIGRGEPIQDTARVLSRYLDGIMIR
                     TYEQDEVEALAKYGSIPVINGLTDLEHPCQVLADLMTIRELKGSLEGLKLCYIGDGNN
                     MANSLIAGAVKTKMMISVACPKGYEPDRGLMEWASENGNFICTTDVMQAAKDADVLYT
                     DVWASMGQESESNIRKAKFKGYQINDSVMSVAKPDAIVLHCLPAHRGEEITEAIFEQH
                     ASHIFEQAENRLHAQKAVMVLLMKDEDE"
     gene            complement(6485..>6760)
                     /locus_tag="PLQ68_07800"
     CDS             complement(6485..>6760)
                     /locus_tag="PLQ68_07800"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HXK72595.1"
                     /translation="RINNKLLSDIIEKEKKIREMLEGAKGIESVSGMGLMLAVKTKKD
                     AKEIVAKCMANGVLYLTAKDRIRMLPPLDIKKKSMEKAIRILKEACK"
     assembly_gap    6761..6860
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6861..7495)
                     /locus_tag="PLQ68_07805"
     CDS             complement(<6861..7495)
                     /locus_tag="PLQ68_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008859970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HXK72596.1"
                     /translation="MNIKKEDKLYIANTYKRFDLDLVKGQKELLYDSNGKEYIDMGGG
                     IAVNIFGASDLQWKDAVISQLNLIQHTSNLYYTYPQVQLAKLLCKKSSMKKAFFSNSG
                     AEANECAIKTARKYSHDLYGNERYFIVTLKNSFHGRTITTLSATGQDMFHKDFGPFTD
                     GFIHVDKDDFASMEEHIKSNKVCAVMLELIQGEGGVIPLDIDFVQKTEKAC"
     gene            complement(7523..8374)
                     /gene="argB"
                     /locus_tag="PLQ68_07810"
     CDS             complement(7523..8374)
                     /gene="argB"
                     /locus_tag="PLQ68_07810"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002852078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HXK72597.1"
                     /translation="MMLTNAQRAQVLVHALPYIQKYNNKIVVVKYGGSAMTQENLKND
                     VMKDIVLMSSIGMKVVLVHGGGPEITNMLNRVGKTTEFVNGLRVTDKETAEIVQMVLC
                     GKINKNLVSMLESYGGKAIGICGHDASLIKAKKINEQLGFVGEITSINKTVIYDLLEK
                     GYIPVISTVGCDDEMNTYNINADTASSAIASALNAECLVSMTDTSGLLLDKLDPDSLI
                     SKVSVSEACKLIREGKIDGGMIPKTECCIEAVRRGVKKVFIIDGRIPHSILIEMLTDE
                     GIGTMFS"
     gene            complement(8420..9634)
                     /gene="argJ"
                     /locus_tag="PLQ68_07815"
     CDS             complement(8420..9634)
                     /gene="argJ"
                     /locus_tag="PLQ68_07815"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008789171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ornithine
                     acetyltransferase/N-acetylglutamate synthase"
                     /protein_id="HXK72598.1"
                     /translation="MEYISDSITAPIGFKANGIHCGIRKNKNKKDLALIVSDTICTTA
                     CVYTQNLVKGAPITVTKQNVKNGFAQAIICNSGNANTCNENGIEIAKLMCEFVAQHLD
                     IDESDVIVASTGVIGQKLSIDPIKNNMNLLCDGLSKENNIKATEAIMTTDTRLKEVAV
                     SFELNSVECRIGGIAKGSGMIHPNMATMLAFLTTDVHISSEMLQKALSDDVEDTFNMI
                     SIDGDTSTNDMVSILANGQSGNIEITEEDANYYIFKDALNAVTSALCQMIAKDGEGAT
                     KLIECKVINGKTKQICKKTAKSVICSSLVKTAMFGADANWGRILCAIGYSGCDVDVEK
                     ISVSFCSDTDAIMVCKNGYGVDFSEELAKKILSQDEITIIVDLNNGDNEATAYGCDLT
                     YDYVKINGDYRT"
     gene            complement(9661..>10410)
                     /locus_tag="PLQ68_07820"
     CDS             complement(9661..>10410)
                     /locus_tag="PLQ68_07820"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="catalyzes the reduction of N-acetyl-5-glutamyl
                     phosphate to N-acetyl-L-glutamate 5-semialdehyde in
                     arginine biosynthesis; Derived by automated computational
                     analysis using gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HXK72599.1"
                     /translation="KESVMLAQNTDTIIIDASTAHRVNPDWTYGLLELGDDIYSSVKT
                     SKRICVPGCHASGFISLVRPLMENSVLSKDYPFSCTSLTGYSGGGKSMIKSYQDINRN
                     ASLSVPNIYALTQTHKHLKEMKQYSGTSQYPVFSPIITDVYSMMCVTVNISLKGTGKN
                     IDDIRNIYIDKYKNRPLIITSDIEALEYEGFIPCDSWKNKDSMKICVTGNDERVSLIA
                     LYDNLGKGASGAAIQIFNTITGCEETKGLQV"
     assembly_gap    10411..10510
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<10511..11847)
                     /gene="argH"
                     /locus_tag="PLQ68_07825"
     CDS             complement(<10511..11847)
                     /gene="argH"
                     /locus_tag="PLQ68_07825"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019662278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HXK72600.1"
                     /translation="MMEKLWKGRFSKDIDKSAEQFNSSINTDCILYRQDILGSIAHGD
                     MLGKTNIIPKVKADSITKCLKEILNDIENGKLNIDMSFEDIHTFVEHELTKRINDAGK
                     MLHTARSRNDQVATDFKMYVKEQMQGMIVYIKSLLSVLADHAEKHIYTVMPGYTHMQR
                     AQPVTLGHYLMAYAMMFLRDLKRYEDFYNSMNTCPLGSGALAGTTYEIDRFMTADALG
                     FDSICLNSIDGVSDRDFAVDACYANSMLMVHLSRFCEELILWASWEFKFIEISDSFST
                     GSSIMPQKKNPDIAELIRGKSARTFSDLNTLLVMLKALPLAYNKDMQEDKEAVFDSFN
                     TAKACVDVFTSMLQETVFLKDNMKKAAQKGFINATDVADYLVKKSMPFRDAYKITGQM
                     VAYCIDNGLVLEKMPIETFKEFCELFDEDVYQHIDLTACVEKRISSGGPSSASV"
     gene            complement(11867..13093)
                     /locus_tag="PLQ68_07830"
     CDS             complement(11867..13093)
                     /locus_tag="PLQ68_07830"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002852294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HXK72601.1"
                     /translation="MEKKIKKIVLAYSGGLDTSIIIPWLKENYEDCEVIAVCGNVGQG
                     KETDGLEAKALATGASKLYIEDLTDEFVNDFIFPTLKAGAVYEKKYLLGTSFARPIIA
                     KRIAEIAIKENADAICHGCTGKGNDQVRFELTIKAFAPDMLIIAPWRIWDIKSRNEEI
                     DYAIEHNIPLKVSRETSYSKDKNLWHLSHEGLDLEDPSKEPLYNKEGFLEMCVSVEKA
                     PNMAEYVTIDFEKGIPVGVNGQKMNGTEVISLLNEIGGKHGIGIVDIVENRLVGMKSR
                     GVYENPGGSILYHAHEVLESITLDRDTMHYKNLVAEKFAELVYYGQWYSPLREALSAF
                     VDNTQQNVTGQVKLKLYKGNIINAGVVSDYSLYDMDIATFEEDDVYNQKDAEGFINLF
                     GLPIKVKAKANIKRNK"
     gene            13455..13631
                     /locus_tag="PLQ68_07835"
     CDS             13455..13631
                     /locus_tag="PLQ68_07835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72602.1"
                     /translation="MNRSVKSKNRISNKGGNNMKGDLFERIITRNKISRMCEKRCNSI
                     DIYEYQHRESRKLI"
BASE COUNT         3880 a         2672 c         2191 g         4759 t
ORIGIN      
        1 cttgacaagt ataatgggaa cctatattat tatatatgaa catatgctca tatgagcgaa
       61 tgttaaagta aatagaagaa ttgaaagaga ggatataaag atggcaattg taaaattcag
      121 tgtaaatgca aaaagtgaga atccaacaaa gacagtagtg gaaacaagag gatttaagat
      181 gatagttgat gaacctgcta atcttggagg aacagatcat ggcgctaatc cggtagaata
      241 tgttttagca gctttatcag gatgcttaaa tgtagttggc cacctggttg caaaagagct
      301 aggtttcacc cttagaggtt tgaatataaa tatagaaggg gatcttgatc ctgctaaatt
      361 cacaggcgcg aaaactaatg agcgtgcagg ttacaaggct ataagagtta atttgaagcc
      421 tgatacagat gctgatgaag caacattaaa aacctggtta gaagttgtag aacaaagatg
      481 tcctgttagt gacaatctaa tgaacacgac accagttact atcagtgtta ataaataggt
      541 ttataatgca ggtttaatta atcctataat tatacaaaac aagtaagaca gaaacaaagc
      601 agcggattat ccgttgcttt gttctattag ttatattttc atatatttaa tttctatata
      661 cttaccgttc cttcacatat aagcactgca ttgcctgtaa gagttattcc ttcgtcagag
      721 tagttgacaa tcagatctcc tcctcttaag tttacagtta tatcggtatt tttctcacag
      781 aagccatttt ccactgctgc tactactgct gcgcaggcac cggtaccgca ggctaaagtc
      841 tctccattgc ctctttccca gactctcatc ttaatggtat ttttgtttac tactcttata
      901 aactcagtat ttattctttc tgggaaaata ggagcattct caaatttcgg tccgatattt
      961 tctacatcaa ccgaatccac acggtcagta aatactacgc aatgaggatt gcctattgat
     1021 acgcaagtta tgttatattc cttatcatca attatgacag gtttgtctat tactgtagga
     1081 gtatcacaga taaccggaat tttatcttta tcaagctctg cttttcccat ttctacagtt
     1141 acagaggata ctttgtcatt tatggtgaac agttttagat tctttatacc tgaagctgtc
     1201 tctatacgca tatcagtctt tttaactata ttattgtcat aaaggtattt agcgacgcat
     1261 cttatggagt ttcctgccat tttgccttca gagccgtctt tattgaatat cctcataaga
     1321 gcatctgctt tctttgactg ctctataaga attaaaccgt ctgagccaat accgaaattt
     1381 cggttgcata aggaaattgc taaagattca gggcagttga ttctcttatc tgaattatca
     1441 aagtagatat agtcattgcc agaaccctgc atcttcacga aattcagttt aagcctttcg
     1501 cttcttaagt tatttatatc cacaagctca gtattattct gattgaatct gctggccaga
     1561 atatcagcta atgcatttgc ggtatctaaa gaggtgagac atggtactga aagctgtata
     1621 gctcttttat gaagccgtat aaagtcctcc atagcgcctc ttgtattagg tattgtacat
     1681 actatgtatt ttatctttcc tgcatccaaa agttttatca aagtgtcatc atatgacaat
     1741 ttatttacaa actgaacatc aatacctaaa ctggatattg cttttgctgt gctgttggta
     1801 gcatatatct taatgtttaa ttcattaaat ttctttgcaa cctttaatat ctccaaccgg
     1861 tcttttttat ctaccgatat gagcactccc tttgatttat ctgcatctac cttaaaacct
     1921 gcagaagtaa gacctttgaa cagtgcttca ttaagggttt ttccaattcc taagacttca
     1981 cctgtagatt tcatctgcgg acctaaagca gagtttgcat cagttagctt ttcaaaggag
     2041 aaaactggta ctttcacagc gaaatatgga ggctgacggt acaaaccggt tccaaatcct
     2101 aaatccttga gtttctgtcc cagcattacc ttggaagcaa gttcaaccat tggaatacct
     2161 gttaccttgg aaatatatgg aacagttctt gaagctctgg gatttacttc aattacatac
     2221 acatcattct cgtatataag atactgtata ttgacaagtc cttttgttcc aagtgataaa
     2281 gctaattttt cagagcagtc tatcaatttg ttaagtaata tatcattaag agtgtaatgg
     2341 ggataaactg ctattgagtc tcctgaatgt attcccgctc tttcaatatg ctccatgact
     2401 ccaggaatca atatatcttc accatcagat attacatcaa cttccagttc agtacccatc
     2461 atgtatttgt ctaccagtac aggattttct atcttgctct taagtatgac attcatatac
     2521 tgctgtatct catcttttgt ataggcaata accatattct gtccgcctat tacatatgat
     2581 ggtcttaaaa gtactggata tcctagtgtt tcagctgcag ttattgcctg ttttgtactc
     2641 attacagcta aacctttagg cctgattata ttaaatctag ataaaagctc gtcaaaacgc
     2701 tctctgtctt cagccatatc tatgccttca gcagaagtgc ccagtatcaa aatgccgtta
     2761 ctatctaaaa atttagtaag ttttatagct gtctgaccac cgaatgctac aacaactccc
     2821 aaaggttttt caaccttaat gacattcatg acatcctctt cagtgagagg ttcgaaatac
     2881 agtctgtcag cagtgttata gtcggtcgat actgtttcag gattattatt tattataatg
     2941 acttcataac ccatattctt taaagcccag acgcagtgta ctgaagaata atcaaattct
     3001 atgccttgtc ctatacgtat tggtcctgaa cctaatacga ttattctttc tttttctgat
     3061 tttttcattg ctcttgcttc acagtactta tcatatgaag aatagaagta aggagttttt
     3121 gcttcaaatt cagcagcaca tgtatcaacc atcttatatg ttgcatctaa agaagggatg
     3181 ttctgcgttt tggaaatcct tttaatggct ttatccgtaa atccgagttt tttcgcttca
     3241 aggtataagt cattggtata ttcattttcc agtttctttt caaaatcagc taaattcttt
     3301 atcttggcta agaaccatct gtcnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3421 nnntcaaatg gccatttagg gaattttaca actacataat ctatagccgg ttcgaaacac
     3481 gcgcatgttt taccggtaat atcatttaac aactcatcaa gggtataccc aagagcaagc
     3541 tgcgctgtaa ctttcgcaat tggatatcca gtagctttgc ttgcaagggc tgatgatctg
     3601 gatacccttg gatttacttc gattactgca taatcaaaac tgttgggatc aagtgcgaac
     3661 tgcacattgc atccgcctat tatctttaac tctgtgataa tattcagtgc ggctgtgcga
     3721 agcatctggt attctttatc tgaaagagta agtgcaggag caacaactat tgagtctcca
     3781 gtgtgcacgc ctacagggtc aagattttcc attgagcata tggcgataac atttccattt
     3841 ttatccctca tagtctcaaa ttcaatttct ttccatccat atatatattt ttctacaagt
     3901 atctgatcta taggggaggc atcaagtcct gattttgcag tagcaagaag ctcctcttcg
     3961 ttatatgcaa cacctccgcc ggtaccgcct aatgtaaaag cgggacgtat tatgacaggg
     4021 tatcctatgc tgtctgctat aagttttgct tttcttacat caacagctat gtcagaaggt
     4081 atgcagggct gatttattga catcatagta tctctgaaaa gctgacggtc ttcagcttta
     4141 tcaatagctt ctacatttgt ccctaaaagg cgtactcctt ctttttgtaa aaaaccgctt
     4201 tttgccagtt gatgggcaat ggtaagaccg gtctgtccgc caaggcctgc tatcatgctg
     4261 tctggttttt cctttttaat aattctcttt atagtttcct gagtaagagg ttcaagatat
     4321 atctcatcgg ctaatgcctt atcagtcatt attgtagcag gattggaatt gaccagtact
     4381 acatcaatac cctcactttt taatacttta cagccttgag caccagcata atcaaattct
     4441 gctgcctgtc ctataattat ggggcctgag ccgattataa gtgtcttttt aatggagtta
     4501 tctcttggca tttctaaaat cctccatcat ttttataaat ttatcaaaaa ggaagcttgc
     4561 atccttaggt ccgctgcatg cctcaggatg aaactgcgag gaaaatgcta aatgatccgg
     4621 atattccata ccttcgcatg taaagtcatt agcatttaca aaactcatat cactttcttt
     4681 tgcaacaaca gagtaaccat gattctgcgt agttatatac acgctgttgg tctttttatc
     4741 tataacaggc tgatttgctc ctctgtgtcc gtatttaagt ttaactgtat catgtcccat
     4801 agccaaggct aaaagctgat ggcctaaaca tataccgaac acaggtacct ttcctacgat
     4861 tttcttaata gtctgaatag cttcaatgtt gtccttggga ttcccaggtc cattggaaag
     4921 cattactccg tctgctttgt aatctaaaat ttcttcgacc atcgcatcat gtggaaggct
     4981 tgtaacatta cagtaccttt tgttgagttc cttgattata ttcttttttg aaccgtagtc
     5041 tatgagtaaa acattgaatt ttggtatagg tatatatgat tcttctattt tctttgaaga
     5101 tacacttgct accgaatcaa aaatcttgta gcttttaatt ttttcaatca tttgttttgt
     5161 tgggcgattg tgtgaaatca tggcattcat aactccatgt tcacgtatta tagtggtaag
     5221 atgtcttgta tctatgtcat atattcctgg tatattctga tttttcagat atgtgttcaa
     5281 atcatactga catctgaagt tagaagggaa gtcgcaaatt tgtctgacta tgtacccttt
     5341 catatgacat cttggacttt caaagtcctc ttcgataatt ccgtaattac ctattaaagg
     5401 gaaggtctga atacatatct gaccgtaata tgaagggtca gtaagcgttt caatataacc
     5461 tcccatacct gtagtgaata caagttctcc aatcgattct atttctgcac caaagctttt
     5521 tccttcaaat acctgattat cttcaagaat caaatatatc aatctttctt tcattcgtcc
     5581 tcatctttca ttaacagtac cattacggct ttttgcgcat gaaggcggtt ctcagcctgc
     5641 tcgaatatgt gtgatgcatg ctgttcaaat attgcttctg taatttcttc tcctctgtgg
     5701 gcaggtagac agtgtaaaac tattgcgtcg ggttttgcca cagacataac cgaatcattt
     5761 atctgatacc ctttgaattt tgcttttctg atatttgatt cgctttcctg acccatagaa
     5821 gcccatacat cagtatatag tacatcagca tcttttgctg cctgcattac gtcagtagta
     5881 cagataaaat ttccattttc cgatgcccat tccatcaaac ctctgtctgg ttcatatcct
     5941 ttggggcatg ctacagatat catcatcttt gtttttacag ctcctgctat taatgagttg
     6001 gccatattat taccatctcc tatgtagcac agtttcagac cttccaaaga acctttaagt
     6061 tcccttattg tcataagatc agctaacacc tgacagggat gctcaaggtc tgttaatccg
     6121 tttataactg gtattgagcc gtatttggcc agtgcttcca cttcatcctg ctcataagtc
     6181 cttatcatga taccatcgag atatcttgat aatactcttg ctgtatcctg tataggttcg
     6241 cctcttccaa tctgaagatc attagcactt aagaaaagag catgtccacc caactcatac
     6301 atgcccgctt caaatgatac ccttgttctt gtagatgatt tttcaaatat catcccaagt
     6361 tttttacctt caaggtattt tttacctatt tcattcttct tttcatattt cagctggtca
     6421 gccagattca atatatcagt tatgtcatcg tcatcaagat cgataagctt taacagatgt
     6481 ttcattattt gcatgcctcc tttaatattc ttatggcttt ttccatagat tttttcttta
     6541 tatccaatgg tggcagcata cggatcctat cctttgctgt aaggtataat accccatttg
     6601 ccatacattt tgcaactatt tctttagcat cctttttagt ttttactgca agcataagtc
     6661 ccataccgct tactgattct atgccttttg cgccttcaag catttctctg atcttttttt
     6721 ccttttctat tatatcactt agcagcttat tgtttattct nnnnnnnnnn nnnnnnnnnn
     6781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6841 nnnnnnnnnn nnnnnnnnnn ttgcaggctt tttctgtttt ttgcacgaaa tcaatatcaa
     6901 gaggtattac tccgccttca ccttgaataa gctctaacat gactgcacat actttgtttg
     6961 attttatgtg ttcttccatg ctcgcaaaat catctttatc aacatgaata aatccatctg
     7021 taaaaggacc gaagtcttta tggaacatat cttgtccggt agctgaaagg gtagttatag
     7081 tccttccgtg aaaactgttt ttcaaagtaa ctataaaata tctttcattt ccataaaggt
     7141 catggctgta ttttcttgct gtttttattg cgcattcatt tgcctctgca cctgaattag
     7201 aaaaaaaagc ttttttcatt gaggattttt tacataaaag ttttgctaac tgtacttgag
     7261 gatatgtgta gtataagttg gatgtgtgct gtataagatt taactgtgat atgacagcgt
     7321 ctttccattg caagtcactt gcaccaaaaa tattcacagc aataccacca cccatatcta
     7381 tatattcttt tccattagaa tcataaagca gttctttctg accttttaca aggtctaaat
     7441 caaacctttt gtatgtattg gctatgtata acttatcttc ttttttaata ttcatatctg
     7501 cacctttact tgtatttttg ttttatgaga acattgtccc tattccttcg tcagtaagca
     7561 tttcaataag tattgagtgg gggatacggc cgtcaattat gaaaaccttt tttactcctc
     7621 ttcttacagc ttctatacag cattcagttt taggaatcat tcctccatcg atttttcctt
     7681 ctctgataag tttgcatgct tctgaaactg acactttgga tatcaaagaa tcaggatcaa
     7741 gtttatctag cagcaatcct gaagtatcag tcatggatac aagacattct gcatttaaag
     7801 cagatgctat agcagatgat gctgtatcag catttatgtt atatgtgttc atctcatcat
     7861 cacagcctac tgtggatatt actgggatat atcctttttc cagaagatcg tatattacag
     7921 ttttatttat tgaagtaatt tctcctacaa agcccagttg ctcatttatt ttctttgctt
     7981 taattaaact tgcatcatgc ccgcatattc ctatagcttt tccgccatat gattccagca
     8041 ttgacactaa attcttattt atcttgccac aaagaaccat ctgaactatt tcagcggttt
     8101 ctttgtctgt aaccctgagt ccgtttacaa attcagtggt tttacctact ctgtttagca
     8161 tattagttat ttccggtccg cctccgtgta caagaacaac cttcatccct attgaggaca
     8221 ttaatacgat atctttcatg acgtcatttt tcagattttc ctgagtcata gcggatccac
     8281 cgtattttac tacaacgatt ttgttgttat atttttgaat atagggtagt gcatgtacaa
     8341 gtacctgagc cctttgagca ttagtaagca taatttttct cctttcaacg aacctctcat
     8401 ttaaaagtca gtgttagttt tatgtacggt aatctccgtt tattttcaca taatcatatg
     8461 taaggtcgca tccataagct gttgcttcat tgtcaccatt gtttaaatcc actattatag
     8521 taatttcatc ctggcttaat atcttttttg caagttcttc agaaaagtcc actccataac
     8581 catttttaca taccataatt gcatctgtgt cggaacagaa agaaacgctt atcttttcta
     8641 catccacatc acatcctgaa taacctatag cgcataaaat tcttccccag tttgcatcag
     8701 ctccgaacat tgcagttttt acaaggcttg agcatataac ggacttggct gtttttttac
     8761 aaatctgctt ggtttttccg ttaatgactt tgcattcaat aagttttgta gcaccttctc
     8821 catcttttgc tatcatctgg caaagagctg aagtaacagc atttagagcg tctttaaaga
     8881 tatagtaatt tgcatcttct tcagttattt ctatatttcc tgactgtccg tttgcaagga
     8941 ttgataccat atcattagta gaagtatctc catcgatact tatcatattg aaagtatctt
     9001 caacatcatc agataatgct ttttggagca tttcactgga tatatgcaca tcagttgtaa
     9061 gaaaagcaag cattgttgcc atattgggat gaatcatgcc tgaacctttg gcaatacccc
     9121 ctattctgca ttctactgaa tttagttcaa aacttactgc cacctcctta agccttgtgt
     9181 cagtagtcat tatggcttca gtagctttta tgttattttc ctttgataat ccatcacaaa
     9241 gaagattcat gttgttttta atagggtcta ttgaaagctt ttgtccgata actcctgttg
     9301 atgcgacaat aacatcagat tcgtctatgt ctaaatgctg tgcaacaaac tcgcacatga
     9361 gctttgcaat ttcaataccg ttttcattgc atgtgtttgc attcccgctg ttgcagatta
     9421 tagcctgggc aaatccgttt tttacattct gttttgtaac tgttatagga gcacctttta
     9481 caagattctg cgtataaacg caagcggttg tgcatatagt atcagaaact ataagggcaa
     9541 gatctttttt gtttttattc ttccttatgc cgcagtgtat accatttgcc ttaaatccta
     9601 ttggtgcagt tattgagtct gatatatatt ccatagtaat ctcctaatca tatttaacat
     9661 ttaaacctgt aaaccttttg tctcctcgca tcctgttata gtattaaata tctgaattgc
     9721 ggctcctgag gctcctttac ccaaattatc ataaagggca atcaaagata ctctttcgtc
     9781 gtttccggta acgcagattt tcattgaatc cttattcttc cagctgtcac aaggtatgaa
     9841 accttcatat tccaaagctt ctatatctga tgtaataatt aacgggcggt ttttgtactt
     9901 atctatgtat atgttgcgaa tatcatctat atttttacct gttcctttta atgatatatt
     9961 tacagttaca cacatcatac tgtatacatc agttatgata ggtgaaaaga caggatattg
    10021 tgatgtcccg ctgtattgct tcatttcttt aagatgctta tgggtctgag taagagcata
    10081 aatgtttgga accgataatg aagcatttct gttaatatcc tgatatgatt taatcataga
    10141 ttttccacct cctgaatatc ctgtaaggga agtacagctg aatgggtaat ctttgcttaa
    10201 aacactgttt tccattaacg gtctgactaa tgatataaat cctgatgcat ggcatcctgg
    10261 tacgcaaatc cgtttactgg tttttactga actataaata tcatcaccaa gctccaatag
    10321 accatatgtc cagtcaggat tgactctgtg agcagtagaa gcatcaatta tgatggtatc
    10381 tgtgttttgt gctaacatta cagattcctt nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10501 nnnnnnnnnn actgatgcag atgaaggacc gccagatgaa attctttttt ctacgcatgc
    10561 tgttaaatct atatgttgat aaacatcctc atcaaacagc tcgcaaaatt ctttaaatgt
    10621 ttcaataggc atcttttcca gtactaatcc gttatctata cagtaagcta ccatctgccc
    10681 ggttatctta tatgcatcac gaaaaggcat ggacttttta actaaataat cagcaacatc
    10741 agtggcatta atgaaccctt tttgtgctgc ttttttcatg ttgtctttta aaaacactgt
    10801 ttcttgaagc atagatgtga acacatcaac acaagcttta gcagtattaa agctatcaaa
    10861 tacagcttct ttgtcttcct gcatatcttt gttgtaggcc aaaggaagag cttttaacat
    10921 aactaaaaga gtattaagat cagaaaatgt ccttgcgctt ttacctctta tgagctctgc
    10981 tatgtctgga ttttttttct gtggcattat agatgagcct gttgaaaaac tatcagatat
    11041 ttcaataaat ttgaattccc atgaagccca caatataagt tcctcgcaaa agcgtgaaag
    11101 atgcaccata agcatgctgt tggcataaca ggcatctaca gcgaaatcgc ggtcagatac
    11161 accgtcaata ctgttaagac aaatagaatc aaaacctaat gcatctgcag tcataaacct
    11221 gtctatttca tatgttgtcc cggcaagtgc gccacttcct aaaggacagg tgttcatgga
    11281 gttataaaaa tcttcatacc tttttaaatc acgcagaaac atcattgcat aagccataag
    11341 gtaatggcct aatgtgacag gctgagccct ttgcatgtgc gtatatccag gcataactgt
    11401 gtatatatgc ttttcagcat ggtcagctaa tactgacaga aggcttttaa tgtaaacaat
    11461 cataccctgc atctgctctt ttacatacat tttgaaatct gtcgcaacct ggtcatttct
    11521 tgaccttgca gtatgaagca ttttgcctgc atcatttatc ctttttgtaa gttcatgttc
    11581 aacaaaggta tgtatgtctt caaaagacat atcaatattg agttttccat tttcgatatc
    11641 attaagtatc tcttttaaac actttgttat actatctgct ttaactttag gtattatatt
    11701 ggttttgcca agcatatccc catgagcaat cgagcctagt atatcctgtc tgtataatat
    11761 gcagtctgtg ttaatagagc tgttaaactg ctctgctgat ttatcgatat ccttgctgaa
    11821 acgtcccttc caaagctttt ccatcattta accttctttt taaatttcac ttatttcttt
    11881 ttatgttagc ctttgcttta accttaatgg gaagaccgaa aagatttatg aagccttctg
    11941 catccttctg gttgtatacg tcatcttctt caaatgtagc tatatccata tcatacagtg
    12001 aataatcaga aacaactcct gcatttatta tatttccttt atacagtttt agttttacct
    12061 gtcctgttac attctgttgg gtattgtcta caaaagctga taaagcttcc ctcaaagggc
    12121 tgtaccactg tccgtaatac actaattcag caaatttctc tgcaacaagg ttcttatagt
    12181 gcatggtgtc acggtctaat gttatacttt ccaaaacttc atgagcatga tatagaatgc
    12241 ttccgcctgg attctcataa acacctcttg atttcattcc gacaagcctg ttttccacta
    12301 tatcaactat tccgatgcca tgttttccac ctatttcgtt taacagtgat atgacttcag
    12361 ttccattcat cttctgtcca tttactccaa ctggtattcc tttttcaaaa tctatagtca
    12421 cgtattctgc catattagga gctttttcaa cagaaacaca catttccaaa aagccctctt
    12481 tgttatataa aggctctttt gaaggatctt ccaaatccag tccttcatgt gaaaggtgcc
    12541 aaagattttt atcttttgag taactggtct ctcttgaaac ctttaatggt atattgtgtt
    12601 ctatagcata gtctatttct tcatttcttg attttatatc ccatatcctc caaggagcga
    12661 taatcaacat atctggagca aatgctttaa tcgtgagttc aaaacgtacc tgatcatttc
    12721 ctttacctgt acagccatgg catattgcat ctgcattttc tttaatagct atttctgcaa
    12781 ttctttttgc aattatcggt cttgcaaagg aagttcctaa tagatatttc ttttcatata
    12841 cggcacctgc ttttaaagta gggaatatga agtcatttac aaattcatct gtcagatcct
    12901 caatatacag ttttgatgct ccagtagcta atgctttagc ttccagtcca tctgtttctt
    12961 tgccctgtcc gacattacca catactgcta tgacttcgca gtcttcatag ttttctttta
    13021 accaggggat gattattgat gtgtccagtc ctcctgagta agctaatacg atttttttaa
    13081 ttttcttttc cattttacgc ctctttaagt taatttagca taattataca gtaaatatga
    13141 ataatgtcaa ctgttttatg aataattatt taaatatatt cagatatatg cataatttat
    13201 gaatatattc atttcctacg aaatatttaa tggttttttg aaaaagcaat taccaaacca
    13261 aaaagttgat taagtaaaaa accaataaaa aaaggtaaat aatggaatta ataggcattt
    13321 ttatactcac tcaaccacca gttgagtaat attacaacct caggttgtga aataacagca
    13381 aaatcaactt ccaatatgtt catatatttt tgctttgaaa ctaaaatgag gttgtgaaaa
    13441 gcaaggagta caagatgaat aggtcagtaa aaagcaaaaa cagaatatcg aacaaagggg
    13501 gtaataacat gaaaggtgat ttatttgaaa gaataattac aaggaacaag atatcaagaa
    13561 tgtgtgagaa aagatgcaac agcatagata tatatgagta tcagcacaga gaaagcagga
    13621 aactcatata aaggataatg aaggagaatg aaaaatgaaa aagatatata actggattag
    13681 aagtattaaa agaacaacag tacaaagcat atctgaaaga agcgacatat atactcagca
    13741 atatcaaatg gcacagaaac tttggtagtt taggtataag aatattgaat taaaggaata
    13801 aa
//
LOCUS       DAOSXM010000059        13752 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2111
            NODE_2824_length_13752_cov_8.544864, whole genome shotgun sequence.
ACCESSION   DAOSXM010000059 DAOSXM010000000
VERSION     DAOSXM010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864178
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 13752)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13752)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:24:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,261
            CDSs (total)                      :: 2,229
            Genes (coding)                    :: 2,218
            CDSs (with protein)               :: 2,218
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 3 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13752
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2824_length_13752_cov_8.544864"
                     /isolate="AMR_MDS_2111"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1299
                     /locus_tag="PLQ68_07840"
     CDS             <1..1299
                     /locus_tag="PLQ68_07840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72603.1"
                     /translation="QTNQGNWLTATGFSTHSADDVWSVATRALTDKAGFTISGTKTTL
                     DALNDLASGDISTACGTALTSYDPPTFAEMTEAFTEIKGVTWSSETDTLEHIRNKQTD
                     IETDTQDLQTQIGTDGVGLTNLPWNSSWDAEVQSEVQDAIEANHLDHLFAADYNPASK
                     PGVATALLNELIESDSGVSRFTANSLEQAPSGGGGATAQQVWEYATRTITNTPDVNVT
                     QIQGHTLAGTGTQIADGLEHFFDVATPAKTMNDCGVAGSGLSAEDVWTYATRVLTGNT
                     NLNDPSAGDIADAVWDEAIADHNTGTTFGGKNQRIVPSETLNDYKADISTLATASALA
                     TVNSIVDAILADTNELQTNQGNWLTATGFSTFDPETDILENTYSYADIFRIWQSGLAG
                     KSTGGGTTEMSFLAIDGETARITATMDENGNRTEITLNGE"
     gene            1299..1487
                     /locus_tag="PLQ68_07845"
     CDS             1299..1487
                     /locus_tag="PLQ68_07845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72604.1"
                     /translation="MANYWAGYFSEYFGADSADYWPIYGISYGLGILIITASISTNIL
                     TISASKSADILTISAEVL"
     gene            1487..1810
                     /locus_tag="PLQ68_07850"
     CDS             1487..1810
                     /locus_tag="PLQ68_07850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72605.1"
                     /translation="MQVLAAGKDKTSYAVSLAFALTSGGAFSLSSITWKLYSASDALI
                     RTATITPVTNPQIILLTPADMSYLNYGNHRTLIVTAIYTSGAGENLTQNAEIKFPVED
                     TPGIT"
     gene            1936..2220
                     /locus_tag="PLQ68_07855"
     CDS             1936..2220
                     /locus_tag="PLQ68_07855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72606.1"
                     /translation="MTICKKCGALFLRKSTLQDLCSNCNVVKRIAHPYKCQIVECQGR
                     CEGYIKEICELCFEYCVAHGWIGWKAVGPEWWEKENLDATYTSCHSDSYL"
     gene            2180..2554
                     /locus_tag="PLQ68_07860"
     CDS             2180..2554
                     /locus_tag="PLQ68_07860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72607.1"
                     /translation="MLHTHHVIRTHIYNKLGITESKRLTLDEIFNFKWSYAFLVGMVN
                     RLLVGGYRHGLNKKVNPEHFLNEAKRRIDLFIKTENPELLFDAANFIMLVYRHGNYEG
                     KKLDTYNHEHYITFQGDYNEKT"
     gene            2541..2957
                     /gene="dut"
                     /locus_tag="PLQ68_07865"
     CDS             2541..2957
                     /gene="dut"
                     /locus_tag="PLQ68_07865"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF001862.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HXK72608.1"
                     /translation="MKKLKTSMSKLQLKKKYSTDAGYDICSAETICIPPKSDILINTD
                     LYIALDTGTVGLVRARSGISINNQIEVGAGVIDADFRGEVKVHLYNLGVNDFHIERGD
                     RIAQLLVLPFISETFYEVDKLPETYRNTNGFGSTGK"
     gene            2960..3685
                     /locus_tag="PLQ68_07870"
     CDS             2960..3685
                     /locus_tag="PLQ68_07870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72609.1"
                     /translation="MSYKPNPKTESSGIIACIPHNTTCPMHCKDCFFQSGRSYLEPLL
                     ENLPNMPTVEEVGHRIVRVNDGNDSNFMRDMVISSTKQYKHKFYNTSMPVHINKFDAP
                     VVLTVNPGVLTDEDFFKLTDTNNLMYVRARTNMWNISNVRNIVEYYTDRKIAVILTFM
                     AYHDINDIPEDYRKYYLCRQRTLNVYYAIKTEAFREIMRMYENNIYVYSCGKLEGEKG
                     TSLCRFCGNCLREYFAALERNSK"
     gene            3682..5187
                     /locus_tag="PLQ68_07875"
     CDS             3682..5187
                     /locus_tag="PLQ68_07875"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HXK72610.1"
                     /translation="MIFVSFYTEDTIYEDGAERLEQCFKKFNLKYHIQPLNLQNKTWQ
                     EICAYRGQFLCDMMDKYHDDVCWIDADATIEQYPENLLNIPKEIDMAFHFRNGQELMM
                     GTSYFRNCQEVRKLLLLWTKTVDPYTTSISQAVFSCLLHSSKYKFNIANIDESYCHIF
                     DTPQSTKHRVEPAVIVHHQFSRQRNSKQSTYRSKRLLEKSKINLPKKGNLPRVALAVN
                     LNGWAFQVRCENLKTHLSKRFDLDIVNPCHLHRIAHNYDLIYFPTYESIQRYKNVCHK
                     ICSTVAGLVVNSLNESIPKMDGASAIIVPNRSWYNAYTKLKVAKPALYYIPHGVDTKL
                     FKPTNLNNHPFTIGWAGNDTMPRAKVKRIQELRDICKKLNIPLVERNFGKNQVPHDEM
                     PNFYNSIDLYVNLSTTEGSNNCILEACSCGVQVLGTPVGNMPELAEHGALLVKPDLSD
                     LVEKIKMVKDTPKHHKKLHGLRLRQCIVNNLSVKHMANAYGDVFDYCLKEK"
     gene            5188..6120
                     /locus_tag="PLQ68_07880"
     CDS             5188..6120
                     /locus_tag="PLQ68_07880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72611.1"
                     /translation="MKIAVYGTKNPPEDDITIRNTSDLRSYFIIRELKARGHEIIFKY
                     FKWDIPVFDEKVDHFLCLLNGGGLSRMPKRVNLHPWKTIRKYTKGKIAEISGGINPKN
                     PIHSDLFFTNIPSNHFNPKVRCIGWAADPILCFPSKPHKLTVLVDHTLYHEGQDETPT
                     IIEGLRKAVLRAKIPMNVIQLNNEGTTVLDFTKEIKIEQYKRDASIPYLDACKLYSSA
                     HIFFVTHAESVGHCVIENAMAGALIAMYPGAIKAPLINPLNHVLYTPGDPNSVYRIIL
                     DAYNKGVNYNKNRKRALPYNWSDATDKIISALEE"
     gene            6122..6745
                     /locus_tag="PLQ68_07885"
     CDS             6122..6745
                     /locus_tag="PLQ68_07885"
                     /inference="COORDINATES: protein motif:HMM:NF025089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXK72612.1"
                     /translation="MKEVYIDTKIVYEPYAPGKDNFSLLGYAYNRAMRDAKDWVLFLD
                     QDVFLINRQWYNIALNAVKAIGHKAGWITAMTNRIGCGAQLVPNMRGVPDDLTLHEKI
                     SMDRYRQFGNKIENITSKTTKLSGFFILTHKQAWEDAGGFNEDLRKGFDNDYCNRVRN
                     AGYKLYRLPGLYVYHACWRKYPRISGIDGHKNDEEMKLLMKAKKKGK"
     gene            6747..7277
                     /locus_tag="PLQ68_07890"
     CDS             6747..7277
                     /locus_tag="PLQ68_07890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72613.1"
                     /translation="MHEACRNWCQLIKDLYPEYFYDSIVCEMGSLNINGSVRDYFTDC
                     TYIGVDVIPGPGVNIVSIFHESPLKKELYDVVTSMNALEHDIYYEKSLKKMVEILKPA
                     GLMFISCSGLWKEHGTINHGANKSGTVKYSEEWSKYYKSLTVNDIVNALEPDENFSVW
                     DCALVERDVRFVGIKR"
     gene            7274..7831
                     /locus_tag="PLQ68_07895"
     CDS             7274..7831
                     /locus_tag="PLQ68_07895"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HXK72614.1"
                     /translation="MKRITTDTYKYCTGGNMREHSFMLYGLVRGIKPKRILEIGVGGL
                     VSGKAMMNAIKDANLACEYHGCDINPDCLALINRFETVDRTFHNMTSNELAKEWNLPL
                     DILLIDGDHSYEQTKIDYENFYPYVNKDGYIIFHDTCPPTEKHKKMTRCGTVYKILDD
                     LYMNHNLEVVTFTYSYGLTVCRKLT"
     gene            7828..8373
                     /locus_tag="PLQ68_07900"
     CDS             7828..8373
                     /locus_tag="PLQ68_07900"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HXK72615.1"
                     /translation="MKQTNRYISCGNNINIKDHTYGVPKIVRRYATDLLEIGSYCSIA
                     SNVTLHYHHNHEFRISLYPFFADPEYKEGPTYKHEAQHIIIGNDVWIGASVNILADVT
                     IGDGAVIGTGAVITKDIPPYAIAVGNPPQIKGYRYDEETIQKLLKIAWWNWPQKTIRK
                     YLPIIQGHDIDRFINKFYKEN"
     gene            8374..9579
                     /locus_tag="PLQ68_07905"
     CDS             8374..9579
                     /locus_tag="PLQ68_07905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72616.1"
                     /translation="MITILQRNDRSKVITDGISVFKEYSNHEVNLHSSVDNEVYFSNL
                     VKSDFVLPCSKIDNKTVKMPLGSYALGDTKELNKEIIRNMLFYISKSDVLNQLDELLH
                     DLTLTGIEHRDINPGNLIFFRESKYIKLIDFYWAKHKNEETKYNPKELNKHYSWDDAT
                     SLLNIKYNFLTIANKIDKEISDSLTLFREKIGIHLHNGSSTVKGKSYHPVSVIGHEFA
                     IKPQKKAAFLEYNHAHKLFNKTKIKSIVDIGAATGFFTFNALRHLHPKTITAFEADPT
                     NFKLLNDIKTIYRLHNLKLSEAFTPKSKIPNKTHITLMLNVHMWLWKAYGEKGVDKIM
                     RNIQQRTKYLLFQTAHAESGGRYRVDKLKTKEDIINYLASFGYDKITLVATTGAHKGI
                     RYMFLCENF"
     gene            9590..9775
                     /locus_tag="PLQ68_07910"
     CDS             9590..9775
                     /locus_tag="PLQ68_07910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72617.1"
                     /translation="MDYLVLLFVCIALSFGFNINKWAEKLKPVDAAVMIVIGIWGFFM
                     TLLLVHYFSLIIKLGVK"
     gene            9772..10413
                     /locus_tag="PLQ68_07915"
     CDS             9772..10413
                     /locus_tag="PLQ68_07915"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HXK72618.1"
                     /translation="MKIDPPDLNRLRKIAGNLMKRHNIPAIISVDDLAQSGAEAYLKH
                     IPRYRDDMKSKPGTFLYSKACFAMKDELRAKGIVFTRTKSKSIHNHRNMSELEDAGIE
                     LKAEGLTIDGHDAIEVQNRVQQLPFKLMLIVIFYYWLNLPYKKIGMMFGLTESRVSQL
                     MKETMEKLQKPAKKLPEPLLPEPKTQKANLLHIEGNIKFLSNCFKIRLEINKN"
     gene            10422..11015
                     /locus_tag="PLQ68_07920"
     CDS             10422..11015
                     /locus_tag="PLQ68_07920"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF018696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HXK72619.1"
                     /translation="MNKLNKVLQADAIWNWYDLEMLRKEVKSADMANYIDRVGYIRKY
                     ARIINTYLPEYVGDISRDILDIGCNNGVFIELMRTLEHRVKGIEKTKSPFMPFYKQSR
                     ISVDCIDVQKMPFSYKDNTFHVVTSIGVITEWYKPADWEKFLNELFRIARRTIVLGVK
                     YNTIYKENKAIIDDFKLPKKWDLVVHNGSFYKWSCAE"
     gene            11165..11392
                     /locus_tag="PLQ68_07925"
     CDS             11165..11392
                     /locus_tag="PLQ68_07925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72620.1"
                     /translation="MKPPLTRKQMELKAEQAGRRHAQNPKNTIRGCPFMQKTLFALLW
                     KQGFTEFREAKLLAEKEVKEGEKECVNNTEK"
     gene            11368..11670
                     /locus_tag="PLQ68_07930"
     CDS             11368..11670
                     /locus_tag="PLQ68_07930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72621.1"
                     /translation="MREQYGKVGSVYIRLAEECAELIQSLMKIERFGPLNFHPTNPTD
                     LNIYAVGREIEDVEIAIKAFRREIYNSFKISELNCAKAKLNVAKEALNETSRSINM"
     gene            11642..12046
                     /locus_tag="PLQ68_07935"
     CDS             11642..12046
                     /locus_tag="PLQ68_07935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72622.1"
                     /translation="MKPVDQSICNFINGDCFRACVASIFEVPLDAIPNFTADGPENFA
                     ALLDKWSTKNNLFCFDVSADSLDPDWIKDMYVIATGPSPRDSSKLHAVVWKNGKLAHD
                     PHPSKLGLADKPTIFTFFTLKDYPKYKIRETL"
     gene            12209..12787
                     /locus_tag="PLQ68_07940"
     CDS             12209..12787
                     /locus_tag="PLQ68_07940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72623.1"
                     /translation="MKNNMTGPLGHFNLSLAIVERYPLIYQTIMRNISVLRAEFLYPM
                     RIAYTGISNMFRQTYIGSEFPMYYLSLGPNGLEANNVAAPTQLAYAILPTGTELPPSV
                     SILASERSDDLHHLIHSDTLPLSPSLEFLTSVPTLCAGTIDAEDARILPSGSIRLSES
                     DGKIVVLSSIFQKVGEYKLQESPIGPVALLKK"
BASE COUNT         4895 a         2197 c         2512 g         4148 t
ORIGIN      
        1 cagaccaatc agggcaactg gctgacggcg actggattca gcactcatag cgcggatgat
       61 gtctggagcg tggcaaccag ggcgctcacc gacaaggccg ggtttacgat tagcgggacg
      121 aaaacaactc tggatgcgct taacgacctt gccagtggtg atatatctac ggcatgtggt
      181 actgccttga catcgtatga tcctcctaca tttgccgaaa tgacagaggc attcaccgaa
      241 ataaaaggag tgacatggtc tagtgaaaca gatacattgg aacacataag aaataaacaa
      301 acagatatag agactgatac tcaagattta cagacacaaa taggaactga tggagtaggt
      361 cttactaatc ttccttggaa ttcttcttgg gatgctgaag ttcaaagtga agttcaagat
      421 gcaattgaag ctaatcattt agatcattta tttgcagctg attataatcc agcttctaaa
      481 cctggagttg ctactgcttt attaaatgag ttaattgaaa gtgattcagg agtttctagg
      541 ttcacagcaa attctttaga acaagctcct agtggaggtg gtggagctac tgcacagcag
      601 gtatgggaat atgctacacg gacaataact aatactcctg atgtaaatgt aacacaaata
      661 caaggacata cattagctgg cacaggaaca cagattgctg atggtctgga acatttcttt
      721 gatgttgcaa caccagctaa aacaatgaat gattgtggag tggctggtag cggactaagt
      781 gcagaagatg tatggacgta tgcaacgaga gtgctaactg gaaatacgaa tcttaatgat
      841 ccgagtgcag gagatatagc ggatgccgta tgggatgagg caatagcaga tcataacact
      901 ggaactacat tcggtggaaa gaaccaaaga atagtaccat ctgaaacact taatgattat
      961 aaagcagata tttccacact tgctacggca tctgctcttg ctactgttaa tagtattgta
     1021 gacgctattc ttgcagatac aaatgaactt caaactaatc aaggtaattg gctcactgct
     1081 actggtttca gcacattcga tcctgaaaca gatatattag aaaacacata ttcatatgct
     1141 gatatattca gaatatggca atcaggttta gctggtaaat ctactggtgg aggaacaaca
     1201 gaaatgagtt tcttggcaat agatggagaa actgcaagaa ttactgctac tatggatgaa
     1261 aatggtaata gaactgaaat aacactaaat ggtgaataat ggctaattac tgggcaggat
     1321 atttttctga atactttggt gcagattctg ctgattattg gcctatctac ggaatatcat
     1381 atggtttagg catacttata ataacagcaa gtatatctac taatatatta actataagtg
     1441 caagtaaaag tgcagatata ttaactatat ctgcggaggt cttataatgc aagtattagc
     1501 tgctggaaaa gataaaacaa gttatgctgt aagtttagct tttgctttaa ctagtggagg
     1561 tgctttttct ttatcatcta taacatggaa actgtattct gcatctgatg cattaataag
     1621 aacagcaaca ataacaccag tcacaaatcc tcaaataata ttattaactc ctgctgatat
     1681 gtcttactta aattatggta atcatagaac acttatagtc acagcaatat atacatcagg
     1741 tgcaggagaa aacctaacac aaaacgctga aatcaaattt cctgtagaag atactccagg
     1801 tattacataa ttgttttgtg caactttctt ataaaaaatt attgttatct aaacagaaaa
     1861 ttttataaca tagcgtatat aataatacat aaacaaaaaa ggataatgta ttatttataa
     1921 atgactgtta tgcaaatgac aatttgtaaa aaatgtggtg ctttgtttct acgtaaatct
     1981 actttacaag atttatgttc taattgtaat gtagtaaaaa gaattgccca cccttataaa
     2041 tgccaaatag tcgaatgtca aggtcgttgt gaaggttata ttaaagaaat atgtgaactt
     2101 tgttttgaat actgtgttgc tcatggttgg ataggatgga aagcagttgg acctgaatgg
     2161 tgggagaagg agaatttaga tgctacatac acatcatgtc attcggactc atatctataa
     2221 taaacttggt ataactgaat caaaacgatt aacactagat gaaatcttta atttcaaatg
     2281 gtcctatgct tttttagttg gtatggttaa tcgtttactt gtaggtggtt ataggcatgg
     2341 actaaataaa aaagtaaatc ctgagcattt tttaaatgaa gctaaaagac gtattgactt
     2401 gtttataaaa acagaaaatc ctgaactact ttttgatgcc gctaatttta tcatgcttgt
     2461 atatagacat ggtaattatg aaggcaaaaa attagataca tataaccatg aacactatat
     2521 cacttttcaa ggagattata atgaaaaaac ttaaaacatc tatgtctaaa cttcagctta
     2581 aaaagaaata ttctactgat gcaggatatg atatatgcag tgctgaaact atatgcatac
     2641 ctcctaaaag tgatatatta attaatactg atttatatat agcactagat actggtactg
     2701 taggtttagt tcgagcacgt agtggaatca gtattaacaa ccagatagaa gtaggcgcag
     2761 gagttattga tgctgatttt cgaggagaag taaaagtaca tctttataat ttaggtgtaa
     2821 atgatttcca tattgagcgt ggagatagaa tagcacaact acttgtattg ccattcattt
     2881 ctgaaacttt ttatgaggta gataaattac ccgaaactta tcgcaataca aatggatttg
     2941 gatctacagg taaatagtta tgagttataa acctaatcca aaaactgaaa gtagtggtat
     3001 tattgcatgt attccccata atacaacttg tcctatgcat tgtaaagatt gtttctttca
     3061 atcaggccgt agttacttag aaccgttact agaaaattta cctaatatgc ctacagttga
     3121 agaagtaggg cataggattg tcagagttaa tgatggtaat gatagtaact tcatgcgtga
     3181 tatggttata tcttctacaa aacaatataa gcataaattc tataatacat ctatgccagt
     3241 gcatattaat aagtttgatg ctcctgtagt gcttaccgta aatcctggag tacttactga
     3301 tgaagatttc tttaaactga cagatacaaa taacttaatg tatgtgcggg ctagaacaaa
     3361 tatgtggaat atcagtaatg tccgtaatat agttgaatat tacacagata gaaaaatagc
     3421 tgtaatacta acttttatgg cataccatga tataaatgat attccagaag attacagaaa
     3481 atattatctg tgcagacaac gtacattaaa tgtgtactat gctataaaaa cagaagcatt
     3541 ccgagaaata atgcgtatgt atgaaaataa tatttatgta tattcttgtg gaaaattaga
     3601 aggcgaaaaa ggaacaagcc tttgtcgttt ttgtggtaac tgcttacgtg aatattttgc
     3661 tgcacttgag aggaactcta aatgatattt gtttcatttt atactgaaga tactatttat
     3721 gaagatggtg ctgaacgcct agaacaatgc tttaaaaagt ttaatcttaa atatcatatt
     3781 cagcctttaa atttacaaaa taaaacatgg caagaaattt gtgcttaccg tgggcaattt
     3841 ctttgcgata tgatggataa ataccatgat gatgtttgct ggattgatgc tgatgcaacc
     3901 atagaacaat atcctgaaaa tttactaaat atacctaaag aaattgacat ggcatttcat
     3961 tttcgtaatg gtcaagaact aatgatggga acttcttatt ttcgtaattg ccaagaagta
     4021 cgtaaattat tattgctgtg gacaaaaaca gtagatccat atactacttc tatttcacaa
     4081 gcagtttttt catgtttgct acatagctca aaatataagt ttaatatagc aaatatagat
     4141 gaatcatatt gtcatatatt cgatacacca caatcaacta aacatagagt agaaccagct
     4201 gttatcgtac atcatcagtt ttctcgtcaa cgtaacagta aacaatctac atatagatct
     4261 aaacgattac ttgaaaaatc taaaataaac ttaccaaaaa aggggaatct tccaagagtt
     4321 gcactagctg ttaatcttaa tggatgggca tttcaagtgc gttgtgaaaa tctgaaaaca
     4381 catttatcaa aaaggttcga tttggatatt gtaaatccgt gtcatttaca ccgtatcgca
     4441 cacaattatg atcttatata ctttcctacg tatgaatcaa tacaacggta taaaaacgtg
     4501 tgtcataaga tttgttcaac agttgcggga ttagttgtta attcattaaa tgaatctatc
     4561 ccaaaaatgg acggagcatc agctatcatt gtacctaatc gttcatggta taatgcttat
     4621 acaaaactta aagtagccaa accggcatta tactatatac ctcatggagt ggatactaag
     4681 ttgtttaaac caacaaactt aaataatcac ccatttacta taggatgggc tggtaatgat
     4741 acaatgccaa gagctaaagt aaaacgcatt caagaattaa gagatatttg taaaaaactt
     4801 aacatacctt tagttgagcg taactttgga aagaaccaag tacctcatga tgaaatgccg
     4861 aatttctata actctattga cctatatgta aatttatcaa caaccgaagg ttccaataat
     4921 tgtattcttg aagcatgttc atgtggagta caagttttag gtacacctgt aggtaatatg
     4981 ccagaattag cagaacatgg agcattatta gtaaaacctg atctatcaga tttagttgaa
     5041 aaaataaaga tggtaaaaga tacacctaag caccataaaa aattacatgg ccttcgttta
     5101 cgtcaatgta tagtaaataa tctgtcagta aagcatatgg ctaatgcata tggagatgtt
     5161 ttcgattact gtctcaagga gaaataaatg aagatagctg tttatggcac taagaatcca
     5221 ccggaagatg atattactat tagaaatact tctgacttac gttcatactt tataatacgt
     5281 gaattaaaag caagaggtca tgaaattata ttcaaatact ttaaatggga cattccagtt
     5341 tttgatgaaa aagtagatca ctttttatgt cttcttaatg gtggcggact tagtcgtatg
     5401 cctaaaagag tcaatttaca tccttggaaa actatacgca aatacacaaa aggaaaaata
     5461 gcagaaataa gtggaggaat aaaccctaaa aatcctatac attcagattt atttttcact
     5521 aatattccta gcaatcattt taatccaaaa gtacgatgta taggatgggc agccgaccct
     5581 atattatgtt ttccttctaa accacataaa cttactgttc ttgtggatca tactttatac
     5641 cacgaaggac aagatgaaac acctacaatt atagaaggct tacgaaaagc tgtcctcaga
     5701 gctaaaattc cgatgaatgt tatacagtta aataatgaag gtactactgt tcttgacttt
     5761 acaaaagaaa taaagattga acaatataaa cgagatgcat ctattcctta tttagatgca
     5821 tgtaaactgt attcctcagc tcatatattt ttcgttactc atgctgaatc agtaggtcat
     5881 tgtgttattg aaaatgctat ggcaggagca ttaattgcta tgtatccagg agcaatcaaa
     5941 gccccactga taaatccact taatcatgtt ttatatactc ctggtgatcc aaattcagta
     6001 tacagaatta ttttagacgc ttacaataaa ggtgtaaatt acaataaaaa cagaaaacga
     6061 gcgttaccgt ataattggag tgacgcaaca gataaaataa tatcagcttt ggaggaataa
     6121 cttgaaagaa gtttatatag acactaaaat tgtatatgaa ccttacgcac caggaaaaga
     6181 caacttttca cttcttggtt atgcatataa tcgtgctatg cgagatgcaa aagattgggt
     6241 tctcttcctc gatcaagatg tattcttaat aaatcgacag tggtataata tagctttaaa
     6301 tgcagtaaaa gctatcggac ataaagcagg ttggattact gctatgacta atcgtatagg
     6361 ttgtggagcc caacttgttc ccaacatgcg aggagtccct gatgatctta cactacatga
     6421 aaaaatcagc atggatcgtt acagacaatt tggtaacaag attgaaaata ttactagcaa
     6481 aacaactaaa ctaagtggat ttttcatact tacgcacaaa caagcatggg aagatgcagg
     6541 tggatttaat gaagacctta gaaaaggttt cgataatgat tactgtaata gagtaagaaa
     6601 tgcaggttat aaactttatc gtcttccagg actttatgtt taccatgctt gttggcgaaa
     6661 atatcctcgc atttccggta ttgatgggca caaaaatgat gaagaaatga agctgcttat
     6721 gaaagctaaa aagaaaggaa aataatatgc atgaagcgtg tagaaattgg tgtcaactaa
     6781 taaaggattt atacccagaa tatttttatg attcaatagt atgtgaaatg ggatcactaa
     6841 atataaatgg ttcagtacgt gactatttta cagactgtac ctatataggt gtagatgtta
     6901 ttccaggacc aggagtaaat atagttagca tttttcatga aagtcccttg aaaaaagaat
     6961 tatatgatgt agttacatct atgaatgcac tagaacatga tatatattat gaaaaatcac
     7021 ttaaaaagat ggtagagata ctaaaaccag ctggtcttat gtttatatct tgttcaggac
     7081 tatggaaaga acatggaaca ataaatcacg gtgcaaataa atcaggtaca gttaaatata
     7141 gcgaagaatg gtctaaatat tataaaagtc tgacagtaaa tgatatagta aatgcgctag
     7201 aacccgatga aaattttagt gtttgggatt gtgcattagt tgaacgtgat gttagatttg
     7261 ttgggataaa aagatgaaga gaataacaac agatacttat aaatattgta ctggtggcaa
     7321 catgagggaa cattctttta tgctttatgg attagtacga ggcattaagc cgaaaagaat
     7381 actagaaatt ggtgttggcg gacttgtttc tggaaaagct atgatgaatg ctattaaaga
     7441 tgcaaaccta gcctgtgaat atcatgggtg tgatataaat ccagattgtc ttgcacttat
     7501 taatagattt gaaactgttg acagaacatt tcataacatg acatctaatg aacttgcaaa
     7561 agaatggaat ctccccctag atattctgct aattgacggt gatcatagtt acgaacaaac
     7621 caaaatagat tatgaaaatt tttatcctta tgtaaataaa gatggataca tcatttttca
     7681 tgatacttgt ccacctacgg aaaaacataa aaagatgact cgttgtggaa cagtatataa
     7741 aatacttgat gatctttata tgaatcataa tctagaagtt gtaacattta cctattctta
     7801 tggacttaca gtttgcagga aacttacatg aaacaaacta atagatatat ttcatgtggc
     7861 aataatataa atattaaaga ccatacctat ggtgtaccta aaattgtgcg ccgctatgct
     7921 actgatctat tagaaatagg atcttattgt tccattgcta gtaatgtcac attacattat
     7981 caccataacc atgaatttag aatatctctt tacccatttt ttgctgatcc tgagtataaa
     8041 gaaggaccaa catacaaaca tgaagcacaa catataatta taggtaatga tgtgtggata
     8101 ggtgctagtg taaatattct tgctgatgtt actatcggtg atggggcagt tataggaaca
     8161 ggtgcagtta taacaaaaga tattcctcca tatgctattg cggtaggcaa tccaccacaa
     8221 ataaaaggct accgttatga tgaagaaact atacaaaaac ttttaaaaat tgcttggtgg
     8281 aactggccac aaaagacaat aagaaaatat ttacctataa tccaaggaca tgacattgat
     8341 cgttttataa ataagtttta taaggagaat taagtgataa caatactaca aagaaatgat
     8401 agatcaaaag taattacaga tggaatatca gtatttaaag aatattctaa ccatgaagta
     8461 aacttgcatt cttctgtaga taatgaagtc tatttttcta acttagttaa atcagatttt
     8521 gtattaccct gtagcaaaat agataataaa actgtaaaaa tgccactagg ttcatatgca
     8581 ttaggtgata caaaagaact taacaaggaa ataatcagaa atatgctatt ttatatctct
     8641 aaatctgatg ttttaaacca actagatgaa ttattacatg atttaacatt aacaggcatt
     8701 gaacatagag atattaaccc tggaaatctt attttctttc gtgaatctaa atatattaaa
     8761 cttattgact tttattgggc taaacataaa aatgaagaaa caaaatataa tccaaaagaa
     8821 ttaaataaac actattcttg ggatgatgca acatcattac tcaatataaa gtataatttt
     8881 ttaacaatag caaacaaaat agacaaagaa atatctgatt ctctcacatt attcagagaa
     8941 aaaataggaa tacatctaca taatggatca tctactgtaa aaggaaaatc atatcaccct
     9001 gtaagcgtta taggacatga atttgctata aaaccacaga aaaaagcggc ttttttagag
     9061 tacaatcatg cccataaact ttttaataaa acaaaaataa aatcaatcgt tgacatagga
     9121 gcggctactg gcttttttac atttaatgca ctacgtcatc ttcatcctaa aactataaca
     9181 gcatttgaag ctgatcctac aaatttcaaa cttttgaatg atataaaaac aatatatcgt
     9241 ctacataatt taaaactttc agaagcattt acaccaaaat cgaagattcc aaacaaaaca
     9301 catataactt taatgcttaa tgttcatatg tggttatgga aagcatacgg tgaaaaaggt
     9361 gtggataaga taatgagaaa tattcagcaa cgcacaaaat atttgttatt ccaaactgct
     9421 catgccgaaa gtggtggacg ttatcgagta gataaattaa agacaaaaga agacatcatt
     9481 aattatcttg catcttttgg gtatgataaa atcactttgg tagcaacaac tggagcacat
     9541 aaaggcattc gttatatgtt tttatgtgaa aacttttgag gaggaagtta tggattattt
     9601 agtattactt tttgtttgta tagcactatc ttttggcttt aatataaata aatgggcaga
     9661 gaaacttaaa cctgttgacg cagcagttat gattgttata ggtatatggg gattttttat
     9721 gactttactt cttgttcatt atttttctct tataattaaa ctgggggtaa aatgaagata
     9781 gacccgccag atcttaaccg cttgcgtaaa atagccggaa acttaatgaa acggcataat
     9841 attccagcta ttatatctgt agatgattta gctcaatcag gagccgaagc atatcttaag
     9901 catatccctc gttatagaga tgatatgaaa agtaaaccag gcacttttct ttatagcaaa
     9961 gcatgttttg ctatgaaaga tgaactacgt gcaaaaggaa tagtatttac tcgtactaaa
    10021 agtaaaagca tacataatca tcgcaatatg tctgaattag aagatgctgg aatagaatta
    10081 aaagctgaag gacttaccat tgatggtcat gatgctatag aagtgcaaaa tcgagtacaa
    10141 caattacctt ttaaattaat gttaatagta attttctatt attggcttaa tttgccctac
    10201 aaaaagatag gaatgatgtt cgggctaact gaatcacgtg tatcacaact aatgaaagaa
    10261 acaatggaaa aactacagaa gccagcaaag aaactgccag agccgttgct accagaacct
    10321 aagacgcaaa aggctaattt gttgcatata gaagggaata taaaatttct gtctaattgc
    10381 tttaaaatta ggttagaaat taataaaaat taggaggaga catgaataaa cttaataaag
    10441 ttttacaggc agatgcaatt tggaattggt atgatctaga aatgctccgt aaggaagtta
    10501 aatcagctga tatggctaat tatattgata gagtagggta tatcagaaaa tatgctcgca
    10561 ttatcaatac atatcttcca gaatatgttg gagatatctc gagggatatt cttgacatag
    10621 gatgtaataa tggagttttt attgaattga tgcgaacact agaacataga gtaaaaggaa
    10681 tagaaaaaac aaaaagtcct ttcatgcctt tctataaaca gtctcgtata agtgtagatt
    10741 gcattgatgt gcagaaaatg ccttttagtt ataaagacaa tacttttcat gttgtcacat
    10801 ctataggtgt aataactgaa tggtataagc ctgctgattg ggaaaagttc ctcaatgagc
    10861 tgtttagaat agctagacgg actattgtac ttggtgttaa gtataataca atatacaaag
    10921 aaaacaaggc tattattgat gattttaagt taccaaaaaa gtgggattta gttgtccata
    10981 atggttcgtt ctataaatgg tcttgtgctg agtaagttgc ataaatcaat ttagatggta
    11041 tggactcatt tcagcaaata ttgaaaacat tgataggtat gggctacaaa aaagcagaca
    11101 taacaaataa attgtatatg aagcattttg tagcttttta ctatttttta aaaaggagaa
    11161 acttatgaaa cccccattaa ccagaaaaca aatggaatta aaagccgaac aagcaggacg
    11221 taggcacgca cagaatccta aaaacactat tcgtggttgc ccatttatgc agaaaacact
    11281 tttcgcactt ctttggaaac aaggatttac tgagttcaga gaagcaaaac tactagctga
    11341 aaaagaagta aaagaaggag aaaaagaatg cgtgaacaat acggaaaagt aggttctgta
    11401 tatatacgtc ttgccgagga atgtgctgag ctaattcaat ctctgatgaa aatagaacgc
    11461 ttcggccctc taaattttca ccctacgaat cctactgatt taaacattta tgcggtagga
    11521 cgtgaaatag aagatgttga aattgctatc aaagcattca gacgagaaat ctacaattca
    11581 ttcaaaatta gcgagcttaa ttgcgctaaa gcaaaactta atgttgctaa ggaggcactc
    11641 aatgaaacca gtagatcaat caatatgtaa cttcattaat ggtgattgct ttcgtgcttg
    11701 cgtagctagt atttttgaag ttcctttaga tgctattccg aattttacag cagatggacc
    11761 agaaaatttt gctgctcttt tggataaatg gagtactaag aataacttgt tctgttttga
    11821 tgtaagtgca gattctctcg accctgattg gataaaagat atgtatgtta tagcaactgg
    11881 tccgagccca cgcgactcat ccaaacttca tgcagttgta tggaaaaacg gcaagctagc
    11941 acatgatcca catccttcta aactcggctt agcagacaaa cctacgattt ttacattttt
    12001 tactttgaag gactatccga aatacaaaat aagggagaca ttatgaaagg actaatttta
    12061 gtcggactcg gagctatggc gataatccta ctagctgttt acatatgcct gcctcaaatg
    12121 ttttcatgtg taagcctaac ctacgggatc gctatggtag tattcgcttt tacctatttc
    12181 gtaataaaaa ttttaaggag aaaaatctat gaaaaataac atgaccggac ctctgggaca
    12241 cttcaacctt tctctcgcaa tagtagaacg atatccactc atctaccaaa cgataatgcg
    12301 taatatatca gtccttcggg ccgagttcct atacccgatg cgaattgcct acactggtat
    12361 ttccaatatg tttagacaaa cttatatagg aagtgaattt ccgatgtact atttatcctt
    12421 aggtccgaac ggactggaag caaataatgt agccgctcct acacaacttg cctatgccat
    12481 tctacccact ggcacagaat tacctcctag cgtctctatc ttagcatcgg agcgaagcga
    12541 cgatctccat cacttaatac actctgatac gcttccactt tccccttcat tagaattcct
    12601 caccagcgtc ccaacgctgt gcgctggcac tattgatgcc gaagatgcta gaatcctgcc
    12661 gtccggtagt atccgactgt ccgagtctga tggaaagatt gttgttctta gcagtatttt
    12721 ccaaaaggta ggagaataca agttgcagga gagtcccata ggtccggttg cccttcttaa
    12781 gaaataatcc ttcccgcctg aacgcacgta tgtaatgtac gtgcgtttaa cattgtttaa
    12841 caaaatttta tgaaaattgt ggaattttgc ccatttttta cataatcgat taacacccgt
    12901 ttaacactta gaatggctct cggtgcggtt ttacaccgat gtttaacaac accctggggt
    12961 gaaagggggg tcatggcata ggttctctat ctatattagt atataataat attattatat
    13021 atagaaccaa tgtgcatacc atggggggtg ttttaccggg ggataccgtt aaacttgccc
    13081 gcaaaatggc tctggaagcc attttaaccg tttaacatcc gtttaatttt ccatgtagaa
    13141 aaatgaccgt tttttaaatt tgcttaaata ctgtttaaca ttgtttaaca ctttatgcaa
    13201 ataaacaaac ttgtttgcaa gggtagcacc cacgtaagag gggagggggg caagctaata
    13261 gtcgaattgg cacgtatttt gctaggcatc tgtgagccgt cactacggca cgaccgaacc
    13321 tagcaacatg cgccattcat taggaacatt cagcattcat taggaacatg cgttattcag
    13381 tagcaacatt gggcattcat tagtaagaga aaatcgccat aggctcggac atgacaaggt
    13441 gtagcaaagt aaaaataacg gtagtaccgg acatggtacg gaatgctaag actaacatgt
    13501 tctagctagt gaaacctgat tatcttagca aaacatcgaa aatcatagca caaacctctc
    13561 accctgaaaa aactatttgt taaacattac tacatgcaag acaaaaatcc gtcatgcaaa
    13621 gtgcaagact tgtcttgcaa gactctgtga gcctttacta tggcatgatt gggaatagtc
    13681 cgcaggcatc cgacggcagg aaaattttat tatgctttat ggaacataaa gatgaaatag
    13741 gcaaaaaaag ac
//
LOCUS       DAOSXM010000060        13704 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2111
            NODE_2840_length_13704_cov_9.701297, whole genome shotgun sequence.
ACCESSION   DAOSXM010000060 DAOSXM010000000
VERSION     DAOSXM010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864178
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 13704)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13704)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:24:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,261
            CDSs (total)                      :: 2,229
            Genes (coding)                    :: 2,218
            CDSs (with protein)               :: 2,218
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 3 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13704
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2840_length_13704_cov_9.701297"
                     /isolate="AMR_MDS_2111"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /locus_tag="PLQ68_07945"
     CDS             <1..189
                     /locus_tag="PLQ68_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006355950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HXK72624.1"
                     /translation="EPPNFVVLEITHTEPGYAGNTAQGATKPATVETGAQIKVPLFIN
                     VGELVRVDTRTGEYMERA"
     gene            189..911
                     /locus_tag="PLQ68_07950"
     CDS             189..911
                     /locus_tag="PLQ68_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007131770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HXK72625.1"
                     /translation="MPINVTIWNEFLHEKNNKAVSDIYPDGIHETLKAYLSEDSDLNI
                     RTATLDQPNHGLTDDVIESTDVLIWWGHMAHMQVSDEIVAKVSDRVRRGMSVILLHSA
                     HASKLMVSLTGCSGSLSWREQNEKQILWNLKPNHPITQGIGEYFVIDKEETYGEPFGI
                     PEPDELIFISWYSGGNVFRSGWIKNIQNGKLFYFQPGHESFPSYKNSQVLLIIKNAVK
                     YLAPLKILDHVESVWQKDPINK"
     gene            988..1776
                     /locus_tag="PLQ68_07955"
     CDS             988..1776
                     /locus_tag="PLQ68_07955"
                     /inference="COORDINATES: protein motif:HMM:NF021445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol lysyltransferase
                     domain-containing protein"
                     /protein_id="HXK72626.1"
                     /translation="MSLAQFSTTYLWRHSTGLLYDIQDDFLYLFEKRYNYSAPLPLGD
                     GDIFKALQNVKEYKEYVGGNNLVYCISEEKKSLFEGKFELSSEPDFAEYVYLSRDLIE
                     LTGKQFHAKKNHLNQFVKNNEYEYKRISSKDIPMVMDLLETWNQKKAYSSDLNIEKQA
                     INELLNYDVGIDYKAGVIISDSRIVAFSIGEQISEDMACVYFEKADVSIKGSYAVINN
                     EFVKNEFSHVKYINRQEDLGIEGLKRAKESYNPVFKVENYSMKL"
     gene            complement(1763..3589)
                     /locus_tag="PLQ68_07960"
     CDS             complement(1763..3589)
                     /locus_tag="PLQ68_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011590235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXK72627.1"
                     /translation="MRAGGGPRATMRLVEKPKDFKGTMKRLVKYLKPYWLLLSISMLF
                     SAASTIFNVLSPRQMGLITTELYIGVTQKAGQIDFAYIGRIIIFLIFLYLAYSLFHVI
                     QGFISNSVAQKIGYELRKNMADKFNKLPLSYFDTTLHGDILSRVTNDIDTITMTFNQS
                     LSQITSTITQVLGVLVMMLIISPLMTGITVLIVPLTSFFVATLVKKSQKYFKAQQKNL
                     GNLNGHVEEMFTGHIVLKVFNGEKRSIDEFKKLNNNLYKSSWKSQFLSGLMMPITGFI
                     GNIGYVAVCVMGGYLAVNNTIQVGDIQAFIRYVRQFNHPMSQTAGIANTLQSTAAAAE
                     RVFEFLNEKEEAKDISNPIELSAIKGNVSFKNVIFGYDKNIQIIKDFSVDISAGQKVA
                     IVGPTGAGKTTLVNLLMRFYDVDSGSIQIDGIDIRDMRRSDLRMLFGMVLQDTWLFNG
                     TISENIGYANPSATKEQIENAANLACAEHFILASGDGYDMLINEEASNISSGQKQLLT
                     IARTVLANNRMLILDEATSNVDTRTEILIQKAMDNLMSNRTSFIIAHRLSTIKNADII
                     LVINDGKIVEKGNHQELLEKNGFYANLYYSQFDKVTISSTVS"
     gene            complement(3649..5376)
                     /locus_tag="PLQ68_07965"
     CDS             complement(3649..5376)
                     /locus_tag="PLQ68_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010966556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXK72628.1"
                     /translation="MGKILAFLKKDLWKIIIILSLTFIQAMANLFLPSFMSDIVNLGI
                     PNGDTAYILEIGGFMLVIAFGGIICQIFARFMSSKVSAGFSMDLRNTVFTKVESFSLT
                     EFDKFSTASLITRCTNDITQIQHFLDMTLRMAITAPMMAIGGIIMAIRYGQSLSLILI
                     VSCTAIIAVMAVNFSITVPKFKMMQTNVDKLNLITRENLSGIRVIRAFNAQATQDKKF
                     DKANQDLTRLHLIIGRIMAALQPLTMLIMNITTVIVLWLGSRLVQSYTIQVGDLMAFI
                     QYVMQIMFSFQMLSMTFMIIPRAMVSVKRINEILDTDPVIKDNNEEDIKTPDNTGKVE
                     FRNVTFYYPNAIEPALKNISFVSKPGQVTAIIGSTGSGKSTLVNLIPRLYDVSEGEVL
                     VDDVNIKNFSQHELRNRIGYVPQKNMLFAGDIESNIVFDREVIESEITESAEISQAME
                     FINEKEQGFQHPIAQAGSNVSGGQKQRLAIARALINSPKIYIFDDSFSSLDFKTESKL
                     RNALEPKVKNSTVILVTQRVSTIMDADQIIVLDSGNIVGVGTHKYLMKNSSVYYEIAS
                     SQLTKEELS"
     gene            complement(5380..5814)
                     /locus_tag="PLQ68_07970"
     CDS             complement(5380..5814)
                     /locus_tag="PLQ68_07970"
                     /inference="COORDINATES: protein motif:HMM:NF013231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HXK72629.1"
                     /translation="MTNLNDTLSNSIHNFYKTLRQKRVTSDLVFHETIILRNILKIQD
                     INKTPIYPSDLSTKLNLSRSYITAVLNSLESKGLVIRIIDSKDRRRIKIDVTSKGRNI
                     FDNMAQRELEQTDLLINELSEEKAVQLINLLDQATQILKKEE"
     gene            5961..7022
                     /locus_tag="PLQ68_07975"
     CDS             5961..7022
                     /locus_tag="PLQ68_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013781425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HXK72630.1"
                     /translation="MDLTGQERISRILKRQPVDRIGLYEHFWGDTHKVWQIPEYIEEK
                     LGFDMIEYWAFNMVADLDYVNKTVSEDEDTITQKDGNYATLRKHKKHNTTPEHIGFDI
                     DSRAKWDELIKPLLFASDRRINFEKYRSKKQYAKDHNMFFVWSGVNAFELMHPITGHV
                     NFLMGMIDDPEWVEDMCMTYAKLTVDLQQILFEKEGYPDGIWYYEDMGFKNSPFISCD
                     MYDRFLYPAHKYTIDYAKSCNLPVIMHSCGFVEPLIPNMLKSGIDCLQVIEVKAGMDL
                     LRIHKNYKERLSLMGGIDVRALYTNDKKIIDEELESKIPFVKEGFGYVLHSDHSIPNT
                     VNFDTYQYFVNRGLELGTY"
     gene            7034..9526
                     /locus_tag="PLQ68_07980"
     CDS             7034..9526
                     /locus_tag="PLQ68_07980"
                     /inference="COORDINATES: protein motif:HMM:NF013256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HXK72631.1"
                     /translation="MENNNNKRKVHLICNAHIDPVWLWNWEEGAAAAISTFKSAVRFC
                     KEYDEFVFNHNEAVLYKWIEEYDPALFEEIKELIKSGKWIVIGGWYLQPDCNMPSGES
                     FVRQIEEGRRYFMEKFGVDGGKTAINFDPFGHTIGLVQIMKDAGYENYIICRPAKLIY
                     DDTDLNNKHIKWTGYNNKSINVFKATSYGSRLGEFKNKYPQLIGNQLDKKDVGVLWGV
                     GNHGGGPSKKDLDDIRNIIEDSDEIVVHTNPDKFFEEYLNRNTHDRTLKKSIHSFSVG
                     CYTSMTKVKQKHRELENKLLLCEKMLSHAVSNNLLENYEHEMMHDAWQDLLFCQFHDS
                     LPGSSIQIVEEDILKRLYHGLEITDRLIYRSYFRLVKGQKPAKEGQYPVLVYNPHPYR
                     VKTNVECEYVLAEQNRIPNTFSKGDVFDENDNIVLCQNEKESSNVPMDWAKKVVFEAE
                     LEPMSVNRFNIVNRIVEKKPYDEVKYDNDYICFDNKSMNVLISKKTGLIDSYIVNGKE
                     YIKKGGCELLVIQDNCDSWGMTVNSYTNIKSRFKLATPKQSAKICGVDKDELDPVRVI
                     ENSELRMIVEANFVYSQSSCNMRYTLNKSNNLIGVNVRLFSQDKNIMYKLNIPTVLTK
                     AKCIGKTSYGRDDLMTNGNENVSHQWLMLKQGTDAFGVINTGTYGSSTKRGEIRMSVL
                     RTPGYSAHPVEDRQILPLDRFSPRIDMGERLYDYYLWAGEYSEIDKLIDMQSLVIHQK
                     PIVLNFNPPEQGDKAKPLCLLSNKSVELVTIRKNENRDGYSLRLFNNLDIKNSVVLSM
                     PIFNIEKEFTLKPFEILTLEYNGQNLKKTKLV"
     gene            9532..>12129
                     /locus_tag="PLQ68_07985"
     CDS             9532..>12129
                     /locus_tag="PLQ68_07985"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HXK72632.1"
                     /translation="MVKKCLNGWWDFYPIYNDDFSMPQEGWLKNAYLVPSVWRKSLEC
                     VKKENEEFFRDATEEDIKNIEVLDFLYDDYNYPKEWTRTKNAWVKTDFFINTVDEDTQ
                     YLILLEAVMPYSKIYINGVHVSEFVHPTLPNEIDISDFIKKGNNELAVLITDYDKDEY
                     GRYMTPTGTMLIAENCGIWQDVYLCRRNKLHIEDCSIRTCCENGKIEVITDIANNGKF
                     NGSIDLYAYIYDKDNKEKLFCIGKETFYIEPSKRQVIKFSGFYKEARLWEPSDPNLYT
                     VVIEIVVDNNLINTYSERFGFRQIEIKGKDILLNNKPIHLFSDWGHKVTTYCYTKEWI
                     SAWFDMIKDCNMNHSRLHTCPHPKIVLDMADEQGILITAETGLHGSGGYQASNSEQYW
                     ENAKDHVIRFIKRDKNHPSLILWSVENEMRWNQKGEEIQYPEKILKHLPEIKNLINEL
                     DPTRPAYHEGDSSLWDESSQDIVSRHYGKDGVGADWWDEKKPLHFGEMALYHYEGPNT
                     AVNICGDKAYTDFKEVDIASGLDAKMLIEYGRTKGISCFGPWNQSCLKLIRYPDKDIS
                     LKYKDYNKPGVKPLFVRAYTSEFAFWKKEKNYRTQKSFNIQKNAFRPFALIDYSLKDS
                     YFDRQTIERNVYLVNDFKDDVCGSLTVFFDNTEILRKEITIKSGYVEKQNILINNNIE
                     SSKEYISEHTLKYSFKSNELVLDEQSFKVNIYNIKSKHIHIFTKKINNRKLSIYNGEV
                     YKDFLTSVNVSFKSFDNIKDINSNNTDILIIGNNTIQDGSTLHENIRKLLEQGVNVLL
                     MEQKLSVFKDINLVNKQYLTAFRTNSKIIKCIKNEHLRFWGEDPYAKVNSNSFISSYN
                     YQKKYTKDDI"
     assembly_gap    12130..12229
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <12230..13219
                     /locus_tag="PLQ68_07990"
     CDS             <12230..13219
                     /locus_tag="PLQ68_07990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72633.1"
                     /translation="NIKLKEMKHIYQGAIKSYDKIISGISNDDLNGVNTYSYSRTSTK
                     NFIISEIVIEKNKKVKSIIETCENSMMKELFIFNGHTELKRAYTISKYSYTDKKRNDY
                     TLLGSINYGKGKIYFNQIEFDTSVEKYKRLQKRFEQNLSYGIEVDSFNYKEIKNIKSS
                     NGYPERVYVSLTDINDFNWPAYVKNTKNNNERMVHKKVYSIGRWDKYENHVMDCALLN
                     SIQDSKQILIAYNIYLSEPRMNSGSNLGIPNPEDLTFLHFNGNGNVRLAINGFDYGSK
                     NFINNSCFYSDISLEKGINYILISWTPKNNEDKLTMQWKNINMKSENAFEFLI"
     gene            13323..>13704
                     /locus_tag="PLQ68_07995"
     CDS             13323..>13704
                     /locus_tag="PLQ68_07995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72634.1"
                     /translation="MKKAKVLSLVLCLILVCSFIFTACDKPTETATVMPTETATETET
                     ATETETETETEEPKEVEKPKVIDATVLYFFGDATDNAGQKLAFKNYVSREYGINFYFH
                     TPARDTYIETINLQAVSGDLTGIVY"
BASE COUNT         4931 a         1876 c         2355 g         4442 t
ORIGIN      
        1 gaaccaccta atttcgttgt gttggaaata actcatactg agccaggtta tgctggtaat
       61 acggcacaag gtgcaacaaa acctgctaca gtggaaactg gtgcacagat aaaagtacct
      121 ttatttatta atgttggtga acttgtaaga gtggatacta gaaccggtga atacatggag
      181 agagcctaat gcctattaat gttactatct ggaatgaatt tcttcatgaa aagaataata
      241 aagcagtaag tgatatttat cctgatggta ttcatgagac tcttaaagct tatttgtcag
      301 aagactccga tttaaatata agaacagcta ctttggatca accaaaccat ggtttaacag
      361 atgatgttat tgaatctact gatgtactta tatggtgggg acatatggca catatgcagg
      421 ttagtgatga aattgttgct aaagtaagcg acagagtcag aagaggaatg tcagtaattc
      481 ttcttcattc agcacatgca agtaaactga tggtttcttt gacaggctgt tcaggaagtc
      541 tttcttggag agaacagaat gagaaacaaa tactatggaa tttgaagcca aaccatccca
      601 taacacaagg tatcggtgaa tattttgtta ttgataagga agagacatat ggtgagcctt
      661 ttggaatacc tgaaccagat gagttgattt ttatctcatg gtattcagga ggaaacgtat
      721 tccgttcagg atggattaag aatatacaaa acggaaagct cttttacttt caacctggac
      781 atgaatcttt tccatcatac aaaaattctc aagttctgtt aattattaaa aatgcagtca
      841 aatatcttgc tccactaaaa atattggacc atgtggaaag tgtttggcag aaagatccga
      901 taaataaata gtatgctcaa tccaaaaccg gtttcacata taaatcatta tgatttaaaa
      961 gattttttta aatatgcaga taaacacatg tcattagcac aattctctac tacatatttg
     1021 tggaggcaca gtacaggtct tttgtatgat attcaagatg atttcttata tttgtttgaa
     1081 aaaagatata attattctgc tcctctgcct cttggtgatg gtgatatttt taaggcttta
     1141 cagaatgtta aagagtataa agaatatgtt ggagggaata atcttgttta ctgtataagt
     1201 gaagaaaaaa aatctctttt cgagggtaaa tttgaattaa gttcagaacc cgattttgct
     1261 gaatatgttt atctatctag agatttaata gaacttacag ggaaacagtt tcatgctaaa
     1321 aagaatcact taaatcaatt tgtaaaaaat aatgagtatg aatacaaacg aatatcatca
     1381 aaagatattc cgatggtaat ggatcttctt gagacatgga atcagaaaaa agcatattca
     1441 tctgatttga atattgaaaa acaagctatt aatgagttgt tgaattatga tgtaggtata
     1501 gactataagg caggggtaat tatttctgat tcaagaattg tagcattttc aattggtgaa
     1561 cagatatctg aggatatggc ctgtgtttat tttgaaaaag cagatgtaag tattaaaggt
     1621 tcatatgctg ttataaataa tgaatttgta aaaaatgagt ttagtcatgt taaatacatt
     1681 aataggcaag aagatcttgg tattgaaggt cttaaacgtg ccaaagaaag ttataatcct
     1741 gtttttaaag ttgaaaatta ttctatgaaa ctgtagaact tattgtcacc ttatcaaact
     1801 gactgtagta tagatttgca taaaatccat ttttttctaa aagttcctgg tgatttcctt
     1861 tttcaacaat ttttccatcg ttaattacaa gaataatatc agcattttta atagtagata
     1921 gtctatgcgc aattataaat gatgttctgt ttgacattaa attgtccata gctttttgaa
     1981 ttaatatttc tgttcttgta tcaacatttg atgttgcctc atcaagaata agcatgcgat
     2041 tattagctaa tactgttctt gctattgtaa gaagctgctt ttgaccagat gaaatattgc
     2101 ttgcttcttc atttattagc atgtcatatc catctcctga tgctagtatg aagtgttctg
     2161 cacaagctaa atttgcagca ttttcaatct gctcctttgt tgctgatgga tttgcataac
     2221 cgatattctc actaatagtt ccattaaaca gccatgtatc ctgaagaacc attccaaata
     2281 acattcgcaa atcacttctt ctcatatcac gaatatcaat tccatcgatt tgtatagatc
     2341 cagaatcaac atcataaaat cgcataagca aattaactaa tgtagttttt ccagctccgg
     2401 ttggtcctac tatagcaact ttttgtccag cgctaatatc aacagaaaag tctttaatta
     2461 tttgaatatt tttatcatac ccgaaaataa catttttaaa tgaaacattt cccttaatag
     2521 cagacaattc aattggattt gatatatctt tcgcttcttc tttttcattt aaaaattcaa
     2581 atactctttc agcagcggca gcagtggatt gcaaagtgtt ggcaattccc gctgtctgac
     2641 tcattggatg attaaattga cgtacatatc taataaatgc ttgaatatca ccaacctgta
     2701 ttgtgttgtt taccgctaaa tagcctccca taacgcaaac tgcaacataa ccaatatttc
     2761 ctataaaacc tgttattggc atcatcaacc cagagagaaa ttgtgatttc catgaacttt
     2821 tatataaatt gttattaagc tttttgaact catcaataga tcttttctca ccattgaata
     2881 ctttgagcac aatatgacct gtaaacattt cttctacatg gccatttaaa ttaccaaggt
     2941 ttttctgctg ggctttaaaa tatttttgag acttttttac taaagtggca acaaaaaaag
     3001 aagttaatgg gactattaga acagttatac cggtcattaa aggtgaaatt ataagcatca
     3061 ttactaaaac tcctaatacc tgtgtaatag tagatgtaat ttgagataaa ctttgattaa
     3121 atgtcattgt tatagtatca atgtcatttg taactctgct taaaatatca ccatgtaaag
     3181 ttgtgtcaaa atagcttaat ggaagtttat tgaacttatc agccatattt ttcctcagct
     3241 catacccgat tttttgagca acactgttgg atataaatcc ttgaataaca tggaaaaggg
     3301 aatacgctaa gtatagaaat attagaaata taataatcct tccgatatat gcaaaatcaa
     3361 tctgcccagc tttttgagtc accccaatat atagttcagt tgtaattaaa cccatttgac
     3421 gtggagaaag aacattgaaa attgttgatg ctgctgaaaa aagcatagaa attgataata
     3481 gtaaccaata aggttttaaa tactttacta atcttttcat agtaccttta aaatcttttg
     3541 gtttttctac aagtcgcatt gttgctcttg gtccgcctcc tgctctcatt ccagggcgta
     3601 ccggaggttt tatatcattc tgggtttctt tcttgttatt tggtgatgtc atgataattc
     3661 ctcctttgtt aattgagatg atgcaatttc ataatataca ctgctattct tcattagata
     3721 tttatgtgta cctactccta caatattacc agaatccagc acaataattt gatctgcatc
     3781 cattattgtg cttactcgtt gagtaacgag gattacggtt gagtttttaa cttttggttc
     3841 caaggcattt ctaagttttg attctgtttt aaaatcaagt gaggaaaaac tatcatcaaa
     3901 tatgtatatt tttggtgagt ttattaaagc tcttgcgatt gccaatcttt gtttctgtcc
     3961 acctgataca ttagaccctg cttgtgctat tgggtgttga aaaccttgct ccttctcatt
     4021 aataaactcc atcgcttgag atatctcagc agattctgtt atttcagatt caataacttc
     4081 tctgtcaaat actatattac tttcgatatc tcctgcaaat aacatatttt tttgaggaac
     4141 ataaccaatt cggtttctaa gctcatgctg tgaaaaattt ttaatattaa catcatcaac
     4201 tagtacctca ccttctgaaa catcatataa tcgaggtatt aagttgacta aagtcgattt
     4261 accactaccg gtagatccta ttatagctgt aacttgtcca ggttttgata caaacgaaat
     4321 atttttaaga gctggttcaa ttgcatttgg ataataaaaa gtaacattac gaaattcaac
     4381 ttttccagta ttatcaggag tttttatatc ttcctcattg ttatccttta taactggatc
     4441 tgtatctaga atctcattta ttcttttaac tgataccatt gctctaggta taatcataaa
     4501 ggtcatgcta agcatttgaa aactgaacat tatttgcata acatattgaa tgaatgccat
     4561 aaggtctcct acctgaattg tataagactg aacaagtctt gaacctaacc ataatactat
     4621 tactgttgtt atattcataa taagcatagt caatggctgt agtgctgcca ttattctacc
     4681 tattatcaga tgaaggcgtg tcagatcttg attggcttta tcaaactttt tgtcttgcgt
     4741 agcttgtgca ttaaatgctc tgattactct tattccgctg agattttctc tggttataag
     4801 attaagttta tcaacatttg tctgcatcat tttgaattta ggaactgtta ttgagaagtt
     4861 aacagccatt actgcaataa ttgctgtaca tgaaactatt aatattaatg ataagctctg
     4921 tccatacctt atagccatta taattccgcc aatagccatc ataggagctg ttatagccat
     4981 tcttaatgtc atgtcaagaa aatgctgtat ctgggtaata tcgtttgtgc acctggttat
     5041 tagtgatgct gtagaaaatt tatcaaattc tgttagagaa aaagattcaa ctttagtaaa
     5101 aacggtattt ctcaaatcca ttgaaaaacc cgcagacact tttgaagaca tgaaacgagc
     5161 aaaaatctgg catataatgc ctccaaaagc tataacaagc ataaatcctc caatttcaag
     5221 aatgtatgct gtatcaccgt tcggtatacc taaatttacg atatccgaca taaagctggg
     5281 aagaaataga ttagccattg cttgaataaa agttaatgac aatataataa ttatcttcca
     5341 taaatctttc tttaagaatg caagtatctt tcccattttt cactcttcct tttttaatat
     5401 ttgagtagct tggtcaagta aatttataag ctgaactgct ttttcttctg aaagttcgtt
     5461 aattaacaga tcagtttgtt ctagttctct ttgtgccata ttatcaaaaa tattccgtcc
     5521 tttagatgta acatcaattt taattcttct tctgtctttg gaatcaatta tgcgtataac
     5581 taaaccctta gattctaggg aatttagtac ggctgttata tatgatcttg agagattaag
     5641 ttttgtgctc aaatcagaag ggtatatagg tgttttattt atatcctgga ttttaagaat
     5701 attacgcaat atgatggttt catgaaatac aagatcgcta gtaactcttt tttgtcttaa
     5761 tgttttataa aaattatgaa tagaatttga aagagtgtca tttaagtttg tcattttggt
     5821 ctccaaaagt agtttttaag aaaaatagtt catatactga attatatttt aaaaattaaa
     5881 attgtcaata ttatttataa ttaattttta taatctatta atattatttt atactgatat
     5941 aatagttagg tgaggtgatt atggatttaa caggtcagga aagaatatca agaatattaa
     6001 aaaggcaacc tgtcgacaga ataggacttt atgaacattt ttggggagac acccacaaag
     6061 tctggcagat accagagtat attgaagaga agcttgggtt tgatatgatc gaatactggg
     6121 catttaatat ggtagctgat ttggattatg taaataagac agtttctgaa gatgaggaca
     6181 caataacaca aaaagatgga aattatgcta cacttagaaa acacaagaaa cataatacaa
     6241 caccagaaca tataggtttt gatattgact cacgggctaa gtgggatgaa ttaattaaac
     6301 cattactttt tgcatcagac agaagaataa atttcgaaaa atacagatcg aaaaaacaat
     6361 atgcaaagga tcataatatg ttttttgtat ggtctggagt aaacgctttt gaactcatgc
     6421 atccaattac aggacatgtt aatttcctta tgggaatgat agatgatcct gaatgggtag
     6481 aagacatgtg tatgacttat gcaaaactga cagtagacct tcagcaaata ctttttgaaa
     6541 aagaaggtta tcctgatggt atctggtatt atgaagatat gggttttaaa aacagtccgt
     6601 ttatatcttg tgatatgtat gacagatttc tatatccagc acataaatat actattgatt
     6661 atgccaaaag ttgtaatctt ccagttataa tgcattcatg cggttttgtg gaacctttaa
     6721 taccgaatat gcttaaatct ggtattgact gcttgcaggt aattgaagtt aaagccggta
     6781 tggatttatt aagaatacat aaaaattaca aagaaagact atcattaatg ggaggcattg
     6841 atgtaagagc cctgtataca aatgataaga aaattattga cgaggaactt gaatcgaaaa
     6901 taccttttgt aaaagaaggt tttggatatg tcttgcattc tgaccattca atacctaata
     6961 ccgtaaattt tgacacatat cagtattttg taaacagagg actagagcta ggaacatatt
     7021 aatggaggtt gacatggaga acaataacaa caaaaggaaa gtgcatctta tatgcaatgc
     7081 acatatagac ccggtttggt tatggaactg ggaagaggga gcagctgcag caatttcaac
     7141 ttttaaaagc gcagttcgtt tctgcaagga gtatgatgaa ttcgtattta atcataatga
     7201 agctgttctt tacaaatgga ttgaagaata tgatcctgct ctttttgaag agattaaaga
     7261 actgataaag tcaggtaaat ggatagtaat agggggatgg tatctgcaac cagattgtaa
     7321 tatgccaagt ggtgagtctt ttgtgagaca gattgaagaa ggaagacgtt acttcatgga
     7381 gaaatttgga gttgatggag gaaaaacagc tattaatttc gatccatttg gtcatacgat
     7441 tggactagtg cagataatga aagatgctgg ttatgaaaat tacataatct gcagacctgc
     7501 aaaacttata tatgatgata ctgatttaaa taataagcac ataaaatgga caggatataa
     7561 caataaatca ataaatgtat ttaaagctac gagttatgga tcgagacttg gcgaatttaa
     7621 aaacaaatat ccacaactta ttggtaatca actggataaa aaagatgtag gagtcttatg
     7681 gggtgtagga aatcatggtg gtggaccatc aaaaaaagat ttagatgata tacgtaatat
     7741 aattgaagat tcagatgaaa ttgtcgtaca tacaaatcct gataaatttt ttgaagagta
     7801 tctaaataga aatacacatg accgtacttt gaaaaaatca atacattcct tcagtgttgg
     7861 ctgttatacc tctatgacaa aagtcaaaca gaagcatagg gaactagaaa ataaactatt
     7921 gttatgtgaa aaaatgctaa gtcatgcagt ttcaaacaat cttctagaaa attacgaaca
     7981 tgaaatgatg catgatgcat ggcaggatct gcttttctgc cagttccatg attctttacc
     8041 aggatcaagc atacaaattg ttgaagagga tattctaaaa agactttatc atggtcttga
     8101 aataaccgac agacttatat acagatctta tttcagactg gttaaaggtc aaaaacctgc
     8161 aaaagaagga caatatccag tattagtata caaccctcat ccatatagag tgaaaacaaa
     8221 tgtggagtgt gaatatgttc ttgcagaaca aaacagaatc ccaaatacat tttccaaagg
     8281 ggatgtattt gatgagaatg ataatattgt attatgtcaa aatgaaaaag aaagttcaaa
     8341 tgttccaatg gactgggcaa aaaaagtcgt atttgaagcc gaattggaac ctatgtccgt
     8401 taatagattt aatattgtaa acagaattgt ggaaaagaaa ccttatgacg aagttaaata
     8461 tgataacgac tatatatgtt ttgataataa gtcaatgaat gttttaataa gtaaaaagac
     8521 tggtttaatt gattcttata ttgtaaacgg aaaagaatat ataaaaaaag gtggatgtga
     8581 attactagta atacaagata actgtgattc atggggaatg actgtaaatt cgtatacaaa
     8641 tattaaaagt agatttaaac ttgcaacacc aaaacaatca gcaaaaatat gtggggttga
     8701 caaagatgaa ttggatcccg tgcgggtaat agaaaatagt gaattaagaa tgatagtcga
     8761 agcaaatttt gtatattcac aatcttcatg caatatgaga tatacattaa acaaatcaaa
     8821 taatcttatc ggggtcaatg taagattgtt cagccaggat aagaatataa tgtacaagct
     8881 gaatattcca actgtattaa caaaagcgaa atgcattggt aaaacctcat atggcagaga
     8941 tgatctgatg acaaatggta atgagaatgt ttcacaccag tggttgatgc tcaaacaagg
     9001 aacagatgca tttggagtta taaacacagg tacatatggg tccagtacaa aaagaggtga
     9061 aataaggatg agcgtattac gtactcctgg ctatagtgct catccggttg aagatcgtca
     9121 gatattgcca ttagacagat tttctccaag aattgatatg ggagaaagat tgtatgatta
     9181 ttacctatgg gcaggtgaat atagtgagat agacaaattg attgatatgc aatcattggt
     9241 tattcaccaa aaaccaatag tactaaactt caatccacct gaacaaggag ataaggcaaa
     9301 gccattatgt cttttaagta ataaatctgt tgaattagtt acgataagga aaaacgaaaa
     9361 tagagatggt tattcactaa gattatttaa taatttagat attaagaatt ctgttgtttt
     9421 atcaatgcca atatttaata ttgagaaaga atttacttta aagccatttg aaatccttac
     9481 acttgaatac aatggacaaa acctaaaaaa gacaaaactg gtatagataa tatggtaaaa
     9541 aaatgtttaa acggatggtg ggatttctat cctatttata atgatgattt ttctatgcca
     9601 caagaagggt ggttaaaaaa tgcatacctt gttccatctg tatggagaaa atctcttgaa
     9661 tgtgttaaaa aagaaaatga agagtttttt cgagatgcaa ctgaagaaga tataaaaaac
     9721 attgaagtac ttgatttttt atatgatgat tataattatc caaaagaatg gactaggact
     9781 aaaaatgcat gggtaaaaac tgattttttt attaatacag ttgatgaaga tactcaatat
     9841 ttaatcttgc ttgaagcagt aatgccgtat tcaaaaattt atattaatgg tgttcatgtg
     9901 tctgaatttg ttcatcccac tttacctaat gaaattgata tatctgattt tattaaaaaa
     9961 ggaaataatg aattagctgt tttaattact gattatgata aagatgaata tggaagatat
    10021 atgactccaa ctggaactat gcttattgca gaaaattgcg gcatttggca ggatgtttat
    10081 ttatgtagga gaaataagct tcatatcgaa gattgttcaa tcagaacttg ttgtgaaaat
    10141 ggcaaaatag aagttattac agatatagct aataatggga aatttaatgg gagtattgat
    10201 ctttatgcgt atatttatga taaggacaat aaggaaaaac ttttttgtat tggaaaagaa
    10261 acattttata tagaaccttc aaagcgacaa gtaatcaaat ttagtggttt ttacaaagaa
    10321 gccagattat gggaaccatc agatcctaat ttgtatacag ttgtaataga aatagttgtt
    10381 gataacaact tgattaatac atattctgaa aggtttggat tcagacaaat tgaaataaaa
    10441 ggaaaagata tattactcaa caacaaacca attcacttat tttcagactg gggtcataag
    10501 gttacaacat actgttatac aaaagaatgg atatctgctt ggtttgatat gattaaagac
    10561 tgtaatatga atcattccag gcttcacaca tgtcctcatc caaaaatagt actagatatg
    10621 gctgatgaac agggtatatt gattactgca gaaactgggt tgcatggttc aggtggatat
    10681 caggcatcaa atagtgagca atactgggaa aatgcaaaag atcatgtcat aagatttatt
    10741 aaaagagata agaaccatcc ttcattaata ttatggagtg tggaaaatga aatgaggtgg
    10801 aatcaaaaag gtgaagagat ccaatatccg gaaaaaatac ttaagcattt accagaaatt
    10861 aaaaacctca ttaatgaact tgaccctaca agacctgcat atcatgaagg ggatagttct
    10921 ttatgggatg aatcatccca ggatatagtt tcacgacatt atggaaagga tggagttgga
    10981 gcagactggt gggatgagaa aaagccactt cattttggtg agatggctct atatcattac
    11041 gaaggtccta atacagcagt aaatatatgt ggtgataaag catatacaga ctttaaagaa
    11101 gtcgacatcg cttctggtct tgatgcaaaa atgttaattg aatatggaag aacaaaagga
    11161 atttcatgtt ttggaccttg gaatcagtcg tgtttgaagt taataagata tccggataaa
    11221 gacatatctc ttaagtacaa agattataat aaacctggag ttaaacctct atttgttaga
    11281 gcatatacaa gtgaatttgc gttttggaaa aaagagaaaa attacagaac gcaaaaatcc
    11341 ttcaatattc agaaaaatgc ttttagacca tttgcactaa ttgattattc tttaaaagat
    11401 tcatattttg atagacagac aattgagagg aatgtttatc tggttaatga ttttaaagat
    11461 gatgtttgtg gatcacttac tgtatttttt gataatacag agattttaag aaaagaaatt
    11521 actataaaat ccgggtatgt tgaaaaacaa aatattttaa tcaataataa tatagaaagt
    11581 tcgaaagagt atatatcaga acatacatta aaatatagtt ttaaatccaa tgaattagtt
    11641 cttgatgaac aatcttttaa ggtaaacata tataatataa aatcaaaaca tattcacatt
    11701 tttacaaaaa agataaataa tagaaaatta agtatttata atggagaagt atataaggat
    11761 tttttaacct cagtaaatgt gagtttcaag agttttgata atattaaaga tataaatagt
    11821 aataataccg atattcttat tattggaaat aataccattc aggatggaag tacattgcat
    11881 gagaatataa gaaagttgct tgagcaaggt gtaaatgtat tattaatgga gcaaaagcta
    11941 tctgttttta aagatataaa tcttgttaat aagcaatatc ttactgcttt tagaacaaat
    12001 agtaaaatta ttaaatgtat taaaaatgaa catttgagat tttggggaga agatccgtat
    12061 gctaaagtta atagtaattc attcatctct tcatataatt atcaaaagaa atataccaaa
    12121 gatgatattn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna atataaagtt
    12241 aaaagaaatg aagcatatat atcaaggtgc aattaaaagt tatgataaaa ttatatcagg
    12301 tatttctaat gatgatttga atggagttaa tacatactca tacagtcgta ctagtacaaa
    12361 aaattttatt atttctgaaa ttgttattga aaagaataaa aaagttaaat ctatcattga
    12421 aacatgtgaa aatagtatga tgaaggaatt atttattttt aatggacata ctgagttaaa
    12481 aagagcttat acaatatcaa agtattctta tactgataaa aaaagaaatg attatacatt
    12541 attaggcagc attaattacg gaaaaggaaa aatatatttt aatcaaattg aatttgatac
    12601 aagtgtcgaa aaatataaaa gattacaaaa gcgtttcgaa caaaatttat catatggcat
    12661 agaagttgat tcatttaatt ataaggaaat aaagaatata aaatcaagca atggttatcc
    12721 tgaaagggta tatgtatcat taactgatat aaatgatttc aattggccgg catatgttaa
    12781 aaacactaaa aataataacg aaagaatggt acataaaaaa gtgtacagta taggaagatg
    12841 ggataagtat gaaaatcatg taatggattg tgctttattg aatagtattc aagattcaaa
    12901 gcagatacta attgcgtata atatatattt atctgagcca cgtatgaact caggaagcaa
    12961 tttaggaata ccaaatccag aggatcttac tttcttgcat tttaatggta atggaaatgt
    13021 tcgattagca attaatggat ttgattatgg aagtaaaaat ttcataaata attcgtgttt
    13081 ctattcagat atatcattag aaaagggaat taactatatt ttaatttcat ggactccaaa
    13141 aaacaatgaa gataaactta caatgcaatg gaagaatatt aatatgaaat ctgaaaatgc
    13201 ttttgaattt ttaatttaac agatttttga ttgtactaaa gacaaaaaac attgacacaa
    13261 atcatatttt tatagtatta tataaatgct gaataagcaa atttaaaaac ggaggggttt
    13321 ttatgaaaaa agcaaaagtt ttaagtttag ttttatgctt aattcttgta tgctcgttta
    13381 tcttcacggc ttgtgataag cctacagaaa cagctactgt aatgccaaca gaaacggcaa
    13441 cagaaactga aacagctaca gaaacagaaa cagaaactga aactgaagaa ccaaaagaag
    13501 ttgaaaagcc aaaagtaata gatgcaacag tactttattt ctttggcgat gccacagata
    13561 atgctggcca gaagcttgcg ttcaaaaact atgtatcaag agaatatgga attaatttct
    13621 atttccatac acctgctaga gatacataca ttgaaacaat caaccttcag gcagtatcag
    13681 gcgatttgac tggtatagtt tatc
//
LOCUS       DAOSXM010000061        13073 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2111
            NODE_3021_length_13073_cov_13.149024, whole genome shotgun
            sequence.
ACCESSION   DAOSXM010000061 DAOSXM010000000
VERSION     DAOSXM010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864178
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 13073)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13073)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:24:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,261
            CDSs (total)                      :: 2,229
            Genes (coding)                    :: 2,218
            CDSs (with protein)               :: 2,218
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 3 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13073
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3021_length_13073_cov_13.149024"
                     /isolate="AMR_MDS_2111"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1223
                     /locus_tag="PLQ68_08000"
     CDS             <1..1223
                     /locus_tag="PLQ68_08000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXK72635.1"
                     /translation="RGRKMGKRAELYIMKYGMTNILRNMPKSIALIAAALVLTISICI
                     ISQTIFIQRQSLESVYTQYSINVSIMDEFCIDSTNLRISSKYYDIISQNQYDIMEYIK
                     DIKVRSIESRGIRYTFDLNGEEQLTGTVTGLTYLPSDDEFGYSFGMLSGYSESVLLGN
                     EAVCIVPNTMLEKMTGQKEGIFLRQSEFSEFVEYKIVGTHEGKPDDIFCSFSSFNENL
                     SRDEYNIYSFGFLLSNAKYNENVAEILKKYFIEPSISGAVTRKANELKLPFRYAYVMP
                     KGNLDDVIKPIESDIQKLMIVEPFLFILSVATGFVVSLLSTKNRKAEFAIMRSLGTGK
                     AMVFFEAFFEQFILSLIGILLGIGVFFVIYQSDTVLVLWKVLIFFACYMFGTAISVLN
                     IAMINVMKIMKSNE"
     gene            1234..1932
                     /locus_tag="PLQ68_08005"
     CDS             1234..1932
                     /locus_tag="PLQ68_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016295072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXK72636.1"
                     /translation="MNKEEKILEVNNVIYEYRNKYQTVKAVNDFSFDFKRGKFYAIVG
                     KSGSGKTTLLSMLAGLDVPKEGSVRYSGKDTKNIDLDKYRRDDVTVIYQDFNLFPLLT
                     AIENVMYPLHLKGIKDKEAKVTAQKMMDSVGLDSSFHKRLPSMLSGGEQQRVAIARAL
                     ASEASVILADEPTGNLDSENGANIIGLLKDLAHTKNYCVIVVTHDLGISSDADQVLSM
                     KDGKLMLEEADKKN"
     gene            complement(1940..4141)
                     /gene="csaB"
                     /locus_tag="PLQ68_08010"
     CDS             complement(1940..4141)
                     /gene="csaB"
                     /locus_tag="PLQ68_08010"
                     /inference="COORDINATES: protein motif:HMM:TIGR03609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046919 - pyruvyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase CsaB"
                     /protein_id="HXK72637.1"
                     /translation="MRIMHLAGGGDIGGAKTHIISLVKELSGSNTLKLVSFRYGDFAK
                     TAKDAGIDVVEIKSGIIFKDIHDLFLIYDNFKPDIVHCHGAKANMMGLFLKNHRGACV
                     TTTIHSDYRKDYMHNIFKQATIGLFNTFALRIIRFHVAVTEQFARTLISRGFEAYNMF
                     SIYNGIDFEKKMPDVSKQDYFEKYKVPYNTDELIIGIAARLTKVKDIPTLIKGFAKAH
                     EKNNNMRLLIGGDGEDKQMLINLSESLGVGEKVHFLGWIDDVDSFFRVIDIDVISSLS
                     ETFPYSLTEGIRAGAIPVSSNVGGATSLIDQKVTGYIFEPRDYETLGDYLIELSQDKN
                     RRVSMQKAVYEHTKANFSLKAMKKKQEEIYSLILKHFNQKERNKVVICGAYGKGNAGD
                     EAIKYTTLLSMRNIDPDMDICVMTLKPKKEKSIDRVQTIYTFNVIKLLFILKKSKIFV
                     SGGGSLIQDVTSSRSLFFYTSTLYLAKKLGCKVIMFGCGIGPISRPFNRRLSGNIIDK
                     YVDVITVRDHLSKEELQSLGVTKPLIEVSADPAVNMNAEKNNTHKIFISEDIPLDGKY
                     ICLALRSWKSFNRYDQIALACEYMYKKFGYITVFFPMEYPKDLTVSEKTAELLSTPYY
                     LIHKRYPTEQVVAFMSKMEQVIAMRLHALIFAAANYVPVVGISYDVKVSGFMSYIESS
                     TCLELEDVTKESLIHAMEESFLHQDKVIKATNALKAKETVNKHQFMRLMEK"
     gene            complement(4149..5258)
                     /locus_tag="PLQ68_08015"
     CDS             complement(4149..5258)
                     /locus_tag="PLQ68_08015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017250456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HXK72638.1"
                     /translation="MKDPRNEKLADMLINYSCRLKEGEKVLISAYETNNDLVKRLIEK
                     AYEAKALPFVQISDESASPKLYENITKEQLEIMNERDLNFMKKMNAYIAIRSSQNIYE
                     MSTVPSKKLSLASKILKPSFDQRVKSTKWCVLRYPNQSMAQLGGMNTEKFADFYYDVC
                     TLDYMKMSDAMDNLVELMNRTDKVHIKGVGTDLSFSIKDIPAIKCAGEMNIPDGEVFT
                     APVRDSVNGYVTFNTPSVHEGKTFENIRLEFEKGKIVKATANYTEHLNKTLDTDEGAR
                     YVGEFAIGVNPYILDPMKDTLFDEKICGSFHFTPGTCYDEAPNGNNSAIHWDLVCIQT
                     EQYGGGEIWFDDTLIRKNGIFVIEQLLCLNPENLK"
     gene            complement(5277..5843)
                     /locus_tag="PLQ68_08020"
     CDS             complement(5277..5843)
                     /locus_tag="PLQ68_08020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72639.1"
                     /translation="MKKFSKTLTLLALILLTLAILSSCKQNIDTTYEITPVLLFELSA
                     FGDSADEYIDYAVHNNGNLIINTDNSFVIDISDYEYTQQSTSKTQTITITKLYIEGAY
                     DKTTLTGNFTGNASITVKRTDIQQSSPEPSDTYEYQINISGTIDTYEETGYINMKYLD
                     EFSYTYNKEPDKLFTGTYRHTITYEIQD"
     gene            5950..6819
                     /locus_tag="PLQ68_08025"
     CDS             5950..6819
                     /locus_tag="PLQ68_08025"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HXK72640.1"
                     /translation="MKRSEILVPIFTPFKEDQSVDYEALKKLVRYVLDKGADGLYTTG
                     SSAETFLLTEEERMKTLEVAAKEADGATIVAHVGAPGTALAIMYAKHAKSVGVDAIAS
                     VPPFYYAHGQEAIKKYYTDIVDACGVKMMVYNIPGATGVNMGVNQINDLLSDDRIYAM
                     KYTAPDYYILNRIVEQSKKPIYSGKDEAFAYALMAGATGAIGTSMNIYPEAFVQIKAA
                     FDKKDIATAQKLQNKLNNLIQPMVDSGTSLAAMKYVSKLVGVDCGGARKPFKELTKEY
                     MIKLEQAVRDNAF"
     gene            6809..7489
                     /locus_tag="PLQ68_08030"
     CDS             6809..7489
                     /locus_tag="PLQ68_08030"
                     /inference="COORDINATES: protein motif:HMM:NF014724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ElyC/SanA/YdcF family protein"
                     /protein_id="HXK72641.1"
                     /translation="MRSKRKKKSRFRLLKAIGIFLLLGILLFTSLQLYVMFYPLNDIM
                     ESIEDLNNIEKPQAVLVLGAGIFASGAPSPVLRDRLDYAFEVYEYYDRKIAIIVSGDN
                     STVEYNETKAMYDYLLSKGVNADDIYKDYAGFDTYDSIYRTEYIFKVNSLIICTQRFH
                     IYRALYIAKRLGFEAYGYAAENKSIYNMTYNHIRESLARVKAVLETDILKRAPKYFGD
                     PIPVKPYD"
     gene            7565..9295
                     /locus_tag="PLQ68_08035"
     CDS             7565..9295
                     /locus_tag="PLQ68_08035"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HXK72642.1"
                     /translation="MEDFRYIDRGKPIPREQYTDQPYVVIADDGAWVCVLTTANGHEG
                     AAGEHVGITISYDKGDSWTDLYFLEGDDVRESAYGVLLKTSFGRIYCFYNFNINNVRN
                     LICRKEHLSHRVDMQGAFVYRYSDDCGKTWSKRYEIPIRETKVDRENPYEGKVRIFWT
                     VGKPFILDNDGYVIIHKIGDMLTISEGWLLRAANMDYEKDPDKLVWETLPDGDVGLIT
                     PKGGGLIAEEQSMVVLSDKSIYCVYRSIDGHPVETYSRDKGHTWDEPQYKKYADGRLM
                     KHPRAANFVWKCNNGKYLYWYHNNGSKWYDNRNPVFLCAGVEYDTSEGKRIKWSQPEI
                     VLYHEDPSVRMSYPDYIEDDGKYYLTETQKLDARVHEIPNNFLESLWNQFDSSDITDE
                     GLLVESEEQSVVEIPKLPEFCVTDRKSPIFATKSLENGVTFEFIYKAGKRKRKIFDNL
                     DSSQKGVLIEQISDYRINIYMNDGINTSCWSSDKEVLKPDERNHIAIIIDGGPNIISY
                     IINGMYNDGKEDRIFGFGRFSRYLKDLNGKKKAYVNKDVKKLRIYDRALLTSEVISNR
                     RGYINKGILK"
     gene            9309..10274
                     /locus_tag="PLQ68_08040"
     CDS             9309..10274
                     /locus_tag="PLQ68_08040"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HXK72643.1"
                     /translation="MKKPIDIVEKVMINGIEQRIHVIGTDINNPLILFIHGGPGVVNR
                     HNVSKYMSGITDKVTIVGWDQRGTGGSYKGAKKEDMNVDTFVEDARELTEYLCKRFNK
                     GKIYIQGHSWGSCVGTLLAYKYPENIKAYFGQGQLVNGIKNEEMSYDYVMEKAKAAGD
                     EKDVQKLLEIKRPVKGLYVNNPIKDLTAQRQLLNKYGGAAVKCSSMWKSTISPILFSS
                     EYTLSDIIGVLKGYSFSLKHMWEEVIKLDFAKDCTRFSIPVFYLIGSSDYNTPFELSY
                     EYFNSIEAPMKDYIWFKESAHSPLAEEPDKFGKTLVEKIELIEAM"
     gene            10413..10679
                     /locus_tag="PLQ68_08045"
     CDS             10413..10679
                     /locus_tag="PLQ68_08045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72644.1"
                     /translation="MLIYGKLIKKNMILTEEKAEYTDSDMTFQQKLTNCLVDICKKMD
                     IQVPIWMNKNTKELAKFNKTFFTDEQFMEKVYFDRFEIEMKRKD"
     gene            10682..11470
                     /locus_tag="PLQ68_08050"
     CDS             10682..11470
                     /locus_tag="PLQ68_08050"
                     /inference="COORDINATES: protein motif:HMM:NF022750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HXK72645.1"
                     /translation="MKRNKIVKTIALALLIVMLSSVLFGCKMQMSEDVFVVGNTLKGS
                     HRLVYSIVNEEQDIDGDLIFKYLVLDNGFTKMQTYADFETWEYRAQVIFNTQTLLPAS
                     SFKSNIYYSEKEEDWSVNTDYTQEKAVIKAVQEDEVAEKSVDKPVQYIDNEMINLTLG
                     LITIEEGQRVDINVLVSESAMFAPYAITNELTETITVNNKSYECVKYTAKYAGFSLFT
                     AKESYLWYTNDDNRILVKYESGSQIMTLKEVIETQADDPWLFEE"
     gene            11470..12039
                     /locus_tag="PLQ68_08055"
     CDS             11470..12039
                     /locus_tag="PLQ68_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HXK72646.1"
                     /translation="MKPDKFLEWLHVIEKMKCNFRHSKTSCKRDESVAEHSYRLILMA
                     YVLKDMIPDVDMSKVVEMCIVHDIGEAITGDIPSFEKTKKHEETEMKEVLEFIQKLPK
                     ERYLHIKSLFEEMEQKKTKEAKVFKALDKMEAVIQHNEADISTWLDIEYDLQKTYGIE
                     EAEPFEFLKDLRQQVLKDTEEKIEKEVKV"
     gene            12273..13055
                     /locus_tag="PLQ68_08060"
     CDS             12273..13055
                     /locus_tag="PLQ68_08060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72647.1"
                     /translation="MENKSAIERKFDIIKESFDGKAFFSVEDAQSVLNEKKSTLYWNL
                     HNMVEKGLLLRIDQGRYSLNYYALDNQPFTSVLADKISKIILETGFRYYISGIDVLLK
                     HMQHIPESYPIMLFVDSYSKEEIEDILLSNGFITINSKELKTRDTIIKLNLQNDLVIL
                     YETEKFDYAKDGFASNEKAFVDLYYEITRRKYPFPIQELARIYQHMEMRGVIDKKKLV
                     QAAYDRNVHYDMRFIAESKYINEEAFKIVEIIRPAIKCQATF"
BASE COUNT         4450 a         2089 c         2614 g         3920 t
ORIGIN      
        1 taagaggaag aaaaatggga aaaagagctg agctatatat tatgaaatat ggaatgacca
       61 acattttgag gaatatgcca aaaagcattg ccctgattgc tgcagcattg gtgctgacaa
      121 tatcgatctg cattataagc caaacgattt ttatacaaag gcagtcttta gaatctgtgt
      181 atacacagta ttcaattaat gtatccataa tggatgaatt ctgtattgat tcaacaaact
      241 taagaatttc ttcaaaatac tatgatatca tttcgcagaa ccaatatgac attatggaat
      301 acataaagga tataaaggta agaagcatag aaagcagggg tataagatat acatttgatc
      361 ttaatggtga agaacagctt acaggtacgg taacagggct gacatatctt ccttcggacg
      421 atgaattcgg atacagtttt ggcatgttgt ctggttattc agagtctgta ttgcttggta
      481 acgaagcagt atgtatagtg ccaaatacaa tgctggaaaa aatgaccgga caaaaagaag
      541 gtatttttct aaggcaaagc gaattcagcg aatttgttga gtataagatt gtaggtaccc
      601 atgaaggaaa accggatgat attttctgtt cattctcaag ttttaatgaa aacctttcaa
      661 gagatgaata caacatatat tccttcgggt ttctcttgtc aaatgctaaa tacaatgaaa
      721 atgtcgctga aatactgaaa aagtacttta ttgaaccctc tatttcagga gctgtaacaa
      781 gaaaagcgaa tgaactaaaa ttaccgttta gatatgctta tgtaatgccg aaaggaaatc
      841 ttgatgatgt tataaaaccc atagaatcgg atattcaaaa acttatgata gttgaaccct
      901 ttttattcat actgtctgtt gctacgggtt ttgttgtgag ccttttgtct actaagaaca
      961 gaaaagcgga atttgctatt atgagaagcc tggggacagg aaaagcaatg gtattttttg
     1021 aagcgttttt tgaacagttt atactaagcc tgataggaat attactgggt atcggagtat
     1081 ttttcgtgat atatcagtct gatacagtac tggttctatg gaaagtatta atattttttg
     1141 catgctatat gttcggtaca gcaatatctg tattaaatat tgccatgata aatgtaatga
     1201 agataatgaa atcaaatgag taaaggagac gaaatgaata aagaagaaaa aatactggaa
     1261 gtcaataatg ttatttatga gtacagaaac aaataccaga cagtgaaagc tgtgaatgat
     1321 ttcagctttg attttaagag aggtaagttt tatgcgattg tcggaaaatc cggatcaggc
     1381 aaaacaaccc ttctgtcaat gcttgccggg ctggatgtac cgaaggaagg ctctgtgcgt
     1441 tatagcggaa aggacacaaa gaatatagat cttgataagt acagaagaga tgatgttaca
     1501 gtaatatatc aggatttcaa tctgtttccc cttcttactg cgattgagaa tgttatgtac
     1561 ccattgcatc ttaagggaat aaaagataag gaagcaaagg ttacagctca gaaaatgatg
     1621 gattcagtcg ggttggattc gtcgtttcat aaaagactac cctcaatgct ttcaggcggt
     1681 gagcagcaga gagtcgcaat agcacgtgcg ctggcctctg aagcatctgt aatacttgct
     1741 gatgagccta ctggtaatct ggattctgag aacggggcga atataatcgg gctgttaaag
     1801 gatcttgccc atacaaagaa ctactgcgtt atagttgtta ctcatgattt agggatatca
     1861 tctgatgcag accaggtgtt aagtatgaaa gatggaaagc ttatgctaga agaagcggat
     1921 aaaaagaact gaacttttat tacttctcca taagcctcat aaactgatgc ttgttaacgg
     1981 tttcttttgc ttttaatgca tttgtagctt ttataacttt atcctgatgt aagaagcttt
     2041 cttccatcgc gtgaattaag gattcttttg tgacatcttc aagttctaag catgttgagc
     2101 tttcaatata gctcatgaaa cctgagacct tgacatcata tgatattcct actacaggaa
     2161 cataatttgc agctgcaaat atgagggcat gaagcctcat ggctataacc tgctccattt
     2221 ttgacatgaa tgcgactacc tgctcagtag ggtatctttt atggattaag tagtaaggag
     2281 tagataacag ttctgctgtc ttttctgata cagtaaggtc ctttggatac tccataggga
     2341 agaatacagt aatatacccg aactttttgt acatgtattc gcaggcaaga gctatctggt
     2401 catatctgtt aaaactcttc cagcttctca aggccagaca gatatatttc ccgtccaatg
     2461 ggatgtcttc tgaaatgaaa atcttatgcg tattattttt ctcggcattc atatttacag
     2521 ccgggtctgc cgacacctcg attaaaggtt ttgttacgcc taaagactgt aattcttctt
     2581 ttgaaagatg atctcgtaca gttattacat caacatattt atcaattata ttcccagaca
     2641 gccttctgtt aaaaggtctg gatatgggtc ctataccgca tccgaacata atcactttac
     2701 atcccagttt tttagcaaga taaagtgtag aagtgtaaaa gaaaagggat cttgatgatg
     2761 ttacatcctg aatcagagaa cctcctccac tgacgaatat ttttgatttt tttaatatga
     2821 acagcagctt tattacattg aaagtatata ttgtctgaac tctgtcaatg gatttttcct
     2881 tttttggttt taaagtcatt acacatatat ccatgtcagg gtcaatgttt ctcatgctta
     2941 aaagagtggt atattttata gcttcatcac ctgcattgcc ttttccgtaa gctccgcata
     3001 ttacaacctt attgcgctcc ttctgattga agtgctttaa tatgagtgag taaatctctt
     3061 cctgcttttt cttcattgct ttcaatgaga agttagcctt tgtatgttca taaacagcct
     3121 tctgcattga tacccgtctg tttttatcct gtgacagttc aataagataa tcgcctaaag
     3181 tctcataatc ccttggttcg aatatatatc ctgtaacttt ctggtcgata agagaggttg
     3241 ctcctcctac attgctcgat accggaatag ccccagccct tatgccttct gtcaaagagt
     3301 aagggaaagt ctcgctaagt gaagatatta catcgatgtc tattaccctg aagaaagaat
     3361 ccacatcatc tatccagcca aggaagtgga ccttttcacc tactccaaga ctttcagaaa
     3421 gattaataag catctgctta tcctctccgt ctccgcctat aaggagcctc atgttattat
     3481 tcttctcatg ggcttttgca aagcctttta taagggttgg gatgtccttg acttttgtaa
     3541 gacgtgcggc aataccaata atcagttcgt cggtgttata tggaactttg tatttctcaa
     3601 aataatcctg ttttgataca tcaggcattt tcttttcaaa atcaataccg ttatatatcg
     3661 aaaacatgtt atatgcttca aatcctctag atatcagggt tcttgcgaat tgctctgtaa
     3721 ctgcaacatg aaacctgatt atcctcaatg caaaggtatt gaacaagcca atggtagcct
     3781 gtttgaatat attatgcata tagtcttttc ggtaatcgga gtgtattgtt gtggtaacac
     3841 atgcacctct gtggtttttt agaaacaggc ccatcatgtt ggcttttgct ccatgacagt
     3901 gcactatgtc aggtttaaag ttgtcatata tcaaaaaaag gtcatggata tctttaaata
     3961 tgataccgct ttttatttcc actacatcaa tacctgcatc ttttgcggtt ttggcaaaat
     4021 ccccataccg aaaggagaca agctttaagg tattcgaacc agaaagctct ttcaccaaag
     4081 aaattatatg ggttttcgct ccaccgatat caccgccacc tgcaagatgc attatcctca
     4141 tatggacctc actttaagtt ttcaggattg agacacagaa gctgttctat tacgaatatg
     4201 ccgtttttgc gtattaatgt atcatcaaac cagatttcgc ctccgccata ctgttctgtc
     4261 tgtatacata ccaaatccca gtgaattgct gagttgtttc catttggagc ttcatcataa
     4321 caggtacctg gagtaaagtg gaaggagcca catatcttct catcaaaaag agtatccttc
     4381 atggggtcta atatatatgg gttgacacct attgcaaatt ctccgacata tcttgcgcct
     4441 tcgtcagtat caagagtctt attcaaatgc tcggtatagt ttgctgtcgc ttttactatc
     4501 ttgccttttt caaattccag acgaatattt tcaaaggttt tgccttcatg cactgaagga
     4561 gtattaaagg taacatagcc gttaactgaa tctcttacag gtgcagtgaa tacctctccg
     4621 tcaggtatgt tcatttcacc agcacattta atggcaggta tgtcttttat agaaaaagac
     4681 aggtcggtac ctactccctt aatatgcact ttatcggtcc tgttcataag ttcaaccaga
     4741 ttatccattg catcgctcat tttcatgtaa tcaagtgtgc atacatcata atagaagtct
     4801 gcaaactttt cggtattcat gcctccaagc tgagccatgg actgattagg gtatcttaaa
     4861 acgcaccatt ttgttgattt aaccctctgg tcaaaggaag gtttcagtat tttagaagct
     4921 aaagatagtt ttttcgatgg aacagtgctc atttcatata tgttctgact ggaacgtata
     4981 gctatatatg cattcatctt cttcatgaag ttcaaatctc tttcattcat gatttccagt
     5041 tgctctttag ttatattctc atacagtttt ggcgaagctg actcgtcaga tatctgtacg
     5101 aaaggcagtg cttttgcttc atatgctttc tctatgagcc tcttaaccag atcgttattg
     5161 gtctcataag ccgatatgag tactttctcg ccttctttaa gcctgcagga gtaattgatt
     5221 aacatatctg ccagtttttc gtttcttgga tctttcattt ttccctccgt aatttatcag
     5281 tcttgtattt catatgttat tgtatgtctg tatgtgcctg taaataactt gtctggttct
     5341 ttattgtatg tatatgaaaa ttcatcaaga tatttcatat ttatataccc agtctcttca
     5401 tatgtatcaa tggttcctga tatattaatt tgatattcgt atgtatctga aggttcagga
     5461 ctgctttgct ggatatcagt cctttttact gtaatagaag catttcctgt aaaattccca
     5521 gtaagggttg ttttatcata agcaccttct atgtataact tagtaatagt aattgtctgt
     5581 gttttagagg tactttgttg tgtatattca taatcagata tatctataac aaaactgtta
     5641 tctgtattta ttatcagatt tccattatta tgcaccgcat aatctatata ctcatctgct
     5701 gagtcaccaa aggcggataa ctcaaacaag aggacaggag ttatctcata agtcgtgtct
     5761 atgttttgtt tacatgaaga caatatagct aaagtcagca gtattaatgc gagcaatgta
     5821 agtgttttgg aaaacttttt cataaatcta ctccgtttag atatatatta tcataaaaca
     5881 tagcatgttt gattgtaaat gatttaagtt tgatttaaac tgtaatataa cacggaggta
     5941 ttaatattta tgaagagatc ggaaatactg gttccaatat tcacaccgtt caaagaagat
     6001 cagagcgttg attatgaagc acttaagaaa cttgtaagat atgttcttga taagggagct
     6061 gacggattgt acacaacagg tagttcagct gaaacttttc ttttaactga agaagaaaga
     6121 atgaagacct tggaagtcgc tgcaaaagaa gctgatggag caactatcgt agctcatgtc
     6181 ggggcacctg gcactgcttt agctattatg tatgcaaagc atgcaaaaag cgtaggagtt
     6241 gatgcaatag cttctgttcc tcctttctac tatgcacacg gacaggaagc tataaagaag
     6301 tactatacag acatagttga tgcatgcggt gtaaaaatga tggtatacaa tatcccagga
     6361 gcaacaggcg taaatatggg agttaaccag ataaatgatc tgttatctga tgacaggatt
     6421 tatgccatga aatatacagc tcctgattac tatatattaa acagaattgt tgagcagtct
     6481 aaaaaaccca tatacagcgg taaggatgaa gcatttgcgt atgcgcttat ggcaggagca
     6541 acaggtgcga taggtacatc catgaatata tatccagaag cttttgtaca gataaaagct
     6601 gcttttgata agaaagatat agcaacagca cagaaattac agaacaagct taataacctt
     6661 atacagccca tggttgacag cgggacttct cttgcggcta tgaaatatgt atcaaaactt
     6721 gtaggtgttg actgcggagg agcaagaaag ccttttaaag agcttacaaa agaatatatg
     6781 ataaaattgg agcaggcagt aagagataat gcgttctaaa aggaaaaaga agagcagatt
     6841 cagactactt aaagcaatag ggattttcct attgcttggt attttactat tcacatcatt
     6901 acagctatac gtgatgtttt atcctctgaa tgatattatg gaatctatag aagatttaaa
     6961 taatattgaa aagcctcagg cagttttggt attaggagcc ggtatatttg caagcggagc
     7021 gccttcacca gtattgagag acaggctgga ctatgctttt gaagtatatg aatattatga
     7081 tagaaagatt gctatcatag tttccggaga taatagcaca gttgaatata acgaaacaaa
     7141 ggcaatgtat gactacctgt tatcaaaggg agtaaatgct gatgatattt acaaagatta
     7201 tgcaggattt gatacatatg acagcatata cagaacagag tatatattca aagtaaacag
     7261 cttaataatc tgtactcaga gatttcatat ttacagagcg ctatacatag caaaaagact
     7321 tggctttgag gcatatggat atgctgcaga gaacaaaagc atatacaata tgacttataa
     7381 ccatataaga gaaagcctgg caagagttaa agccgttctt gagacagata tactaaaaag
     7441 agcgccaaag tattttggtg accctatacc tgtaaaacca tatgattagg tttcattgac
     7501 cgggtattta tacaatgata atatatacat atccaaatac ctaagtatac aaagaggtgg
     7561 caaaatggaa gatttccgat acatagacag aggaaaaccc atacccagag agcagtatac
     7621 cgaccagccg tatgtagtga tagctgatga cggagcatgg gtctgcgttc ttacaactgc
     7681 taatggtcat gaaggtgcag caggagagca tgtaggtata actataagct atgataaggg
     7741 tgattcctgg acagatttgt attttcttga aggtgatgat gtaagggagt cggcttatgg
     7801 agtcctttta aaaacatcat ttggcagaat ctactgtttc tataacttca acataaataa
     7861 cgtgagaaat ttaatatgca gaaaagagca cctgtcacac agagtggata tgcagggagc
     7921 ttttgtatac agatattctg atgactgcgg caagacatgg tccaaaagat atgaaatacc
     7981 aataagggaa accaaagtgg acagggagaa tccgtatgaa ggtaaagtca ggatattctg
     8041 gacagtcgga aaacctttta ttcttgacaa tgatggttat gtcataatcc ataagatagg
     8101 cgatatgctt acaatatctg aaggatggct gttacgtgca gctaatatgg attatgaaaa
     8161 agacccagat aagcttgtgt gggagactct gccagatggc gatgtcggac ttattacccc
     8221 aaaaggagga ggattaattg ctgaagagca gtccatggtg gttcttagcg ataagagcat
     8281 atactgcgta taccgttcca tagatggcca ccctgtagag acttacagtc gtgataaagg
     8341 gcatacatgg gatgaacctc aatacaaaaa gtatgctgat ggaagactaa tgaagcatcc
     8401 aagagctgca aacttcgtgt ggaagtgtaa taacggcaaa tacctgtact ggtatcataa
     8461 caatggtagt aaatggtatg acaacaggaa cccggtgttt ttatgtgcag gtgttgagta
     8521 tgatacatca gaaggcaaaa gaataaaatg gtcacaaccc gaaatcgtac tttatcatga
     8581 agacccgagt gtcagaatga gttatccgga ctatatagaa gatgacggaa aatattatct
     8641 tacagaaaca caaaaactcg acgcaagagt gcatgagata cccaacaact ttttagaaag
     8701 cctttggaac cagtttgatt catcagatat aacagatgaa gggcttcttg tagaaagtga
     8761 agagcaatct gttgttgaaa taccgaaatt gccggaattc tgtgttactg acagaaaaag
     8821 tcccatattt gcaacaaaga gcttggaaaa tggtgtaaca tttgaattca tatacaaagc
     8881 aggaaagagg aaaaggaaga tatttgataa tcttgattct tctcaaaaag gagtgctgat
     8941 tgaacagata tcagattaca ggattaacat atatatgaat gacggtataa atacaagctg
     9001 ctggagttct gataaagaag tgcttaaacc agatgaacga aatcacattg ccataattat
     9061 tgatggtgga cctaatatca tatcttacat aataaatggc atgtataatg acggaaaaga
     9121 agacaggata ttcggattcg gacggttcag cagatattta aaggatctga atggaaagaa
     9181 aaaagcatat gtaaacaaag atgttaagaa attaaggatc tatgacagag cactattaac
     9241 atcagaggta atttctaaca gaagaggata tattaataaa ggcattttaa aatagaggag
     9301 acaatgatat gaaaaaacca atagacatag tcgaaaaggt aatgattaac ggtatagagc
     9361 agaggattca tgtaattgga acagatatta acaatcctct tattcttttc atccatggag
     9421 gacctggcgt agtaaacagg cacaatgtat caaaatacat gtcaggcata actgacaaag
     9481 tgacaatagt cggttgggac cagagaggca caggcggaag ctataaaggg gccaagaaag
     9541 aagatatgaa tgtggatacc tttgtagaag atgcaaggga actgacggaa tatctttgta
     9601 aaagattcaa taagggtaag atatacatac aaggacattc ctgggggtca tgtgtcggca
     9661 cgctgttagc atacaaatat ccagagaata taaaagcata ttttgggcag ggacagcttg
     9721 taaacggcat taagaacgag gaaatgagct atgattatgt aatggaaaaa gcgaaagctg
     9781 ctggtgatga gaaagatgtc cagaagcttt tggaaataaa aagacctgta aaaggacttt
     9841 atgtaaacaa cccgataaaa gatcttacag cacaaaggca gcttttgaat aaatacggag
     9901 gagcagcagt taaatgttct tcaatgtgga aaagcactat tagtccgata cttttttcat
     9961 cagaatatac attgtctgac ataataggag ttttaaaagg ttacagcttt tctttaaagc
    10021 acatgtggga agaagtaatt aaactggact ttgccaaaga ctgcacaagg ttttctatac
    10081 ctgtgtttta tctgataggg tcttctgact acaatacccc atttgaactg tcatatgaat
    10141 actttaattc aattgaagca cctatgaaag actatatatg gtttaaagaa tcagcacaca
    10201 gtccgttagc tgaagaacca gataaatttg ggaaaactct tgtagaaaaa atcgaactga
    10261 tagaagcgat gtagtacatt taaagataat atgtttttaa aacatcaatt caatggtaga
    10321 atagacaata gctaagagca atagctaaaa gcaatgcttt attcaattgc tcttggatat
    10381 tactcaaagt gtttgtacaa aaaggataaa taatgcttat atacggaaag ctaattaaaa
    10441 agaatatgat actgactgaa gaaaaagctg aatatactga ttcagacatg acttttcagc
    10501 agaagcttac aaactgtctt gtagacatat gcaagaagat ggacatacag gtccctatat
    10561 ggatgaataa gaatactaaa gaactggcta agttcaacaa gacatttttt acagatgagc
    10621 agtttatgga aaaagtatat tttgacagat ttgaaataga gatgaaaagg aaggattaaa
    10681 tatgaagaga aataagatag taaaaacaat agctcttgct ttattgatag tgatgctttc
    10741 atcagtcttg ttcggatgca agatgcagat gagtgaagat gtatttgtag ttggtaatac
    10801 gctaaaggga tcacacagac ttgtatattc aatagtaaat gaagaacagg atattgacgg
    10861 agacctaata ttcaaatatt tagttttgga taatggtttt acaaaaatgc agacatatgc
    10921 tgattttgaa acatgggagt atagagcaca ggtaatattc aatacacaga ctcttttacc
    10981 ggcttcttca ttcaaatcca atatatatta ttctgaaaaa gaagaagact ggtctgtaaa
    11041 tacagattac acgcaggaaa aggcagttat taaagcggtc caggaagatg aagtagctga
    11101 aaaatcggta gataaacccg ttcaatatat tgacaatgaa atgattaatc tgactttagg
    11161 attaattaca atagaagaag gacagagggt ggatataaat gtgctggtca gtgaaagcgc
    11221 aatgtttgct ccttacgcaa taaccaatga attgactgaa acaattactg ttaataacaa
    11281 gtcatatgag tgtgtcaaat atacagccaa gtatgcgggg ttttcgctgt ttacagctaa
    11341 agagagctat ctgtggtaca caaatgatga taacaggatt cttgtaaaat atgaaagcgg
    11401 ttcacagata atgactttga aagaagttat tgaaacacag gctgatgacc catggttatt
    11461 tgaggaataa tgaaacctga taaattttta gaatggctac atgttattga gaagatgaaa
    11521 tgcaattttc gacattcaaa gacatcctgt aaaagagatg aatctgtagc agagcactca
    11581 taccgcctta tactcatggc atatgtatta aaggatatga tacctgatgt ggacatgagc
    11641 aaggtagttg aaatgtgcat agttcatgat ataggcgaag ctataacagg agatattcct
    11701 tcttttgaga aaactaaaaa acatgaggaa acggaaatga aggaagttct ggagtttata
    11761 caaaagcttc ctaaagagag atatcttcac attaaaagtc tttttgaaga aatggaacaa
    11821 aagaagacaa aggaagcaaa agtctttaaa gcattggata agatggaagc tgttatacag
    11881 cacaatgaag cagatatatc tacatggctg gatatcgagt atgatcttca gaaaacctat
    11941 ggtatagaag aagcagaacc ttttgaattc ttaaaagatc taagacaaca ggtactgaaa
    12001 gatactgaag agaagataga aaaagaagtt aaagtataaa acaaaaagac tggtataatg
    12061 accaagattt ataaaaaata gcaaattcaa aggaaatccg gcaaatatca tctttgccgg
    12121 ttatttattg catttttaaa gtaattatta atttactgta aaatatgcaa tatattagag
    12181 taaacaaaga atttacggca aatatcatct ttgccgtaaa attattgaat tttaaagtaa
    12241 actatggtat tctattaata ggggagatga acatggaaaa taaaagtgca attgaaagga
    12301 aatttgatat tataaaggag tcatttgacg gaaaagcttt tttcagtgta gaagatgcgc
    12361 agagtgtatt aaatgaaaag aaatcaacac tctattggaa tctgcataac atggttgaga
    12421 aggggttatt attacgtatt gatcaaggta gatattcact taattattac gcattagaca
    12481 accagccttt tacatcagtg cttgcagata agattagtaa gattatattg gaaacaggct
    12541 ttcggtacta tatttcaggt attgatgtat tacttaagca tatgcagcat attcctgaga
    12601 gttatccgat aatgcttttt gttgacagct acagtaaaga ggaaattgaa gatattttat
    12661 taagcaatgg atttataact atcaactcaa aagaattaaa gacaagagat actattataa
    12721 aactaaattt acagaatgat ctcgttatcc tgtatgaaac tgagaaattc gattatgcaa
    12781 aagatggttt cgcttcaaat gagaaagcgt ttgtagattt atactatgaa attaccagaa
    12841 gaaaatatcc ttttcccata caagagctgg caagaatata tcagcatatg gaaatgaggg
    12901 gagtaattga taaaaagaaa cttgtacaag ctgcatatga cagaaacgtt cattacgata
    12961 tgcgttttat cgcagaaagt aaatatataa acgaagaagc cttcaaaatc gtagaaataa
    13021 taagaccagc tataaaatgt caagcgacat tttaagataa aaaacctaaa agc
//
LOCUS       DAOSXM010000062        13067 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2111
            NODE_3024_length_13067_cov_9.723717, whole genome shotgun sequence.
ACCESSION   DAOSXM010000062 DAOSXM010000000
VERSION     DAOSXM010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864178
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 13067)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13067)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:24:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,261
            CDSs (total)                      :: 2,229
            Genes (coding)                    :: 2,218
            CDSs (with protein)               :: 2,218
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 3 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13067
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3024_length_13067_cov_9.723717"
                     /isolate="AMR_MDS_2111"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(17..271)
                     /locus_tag="PLQ68_08065"
     CDS             complement(17..271)
                     /locus_tag="PLQ68_08065"
                     /inference="COORDINATES: protein motif:HMM:NF027585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4914 family protein"
                     /protein_id="HXK72648.1"
                     /translation="MGYSLDSLKVDGQYIRKVLLQPHLQVEVANEGYDEGSKILTDFF
                     KKEIIKFDTPSLKPLGHEIIKLLLNDATVDEYQSLITMKH"
     gene            complement(316..777)
                     /locus_tag="PLQ68_08070"
     CDS             complement(316..777)
                     /locus_tag="PLQ68_08070"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006440636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HXK72649.1"
                     /translation="MNSYCYDTKIGRICITEDDIGISCISMSCNDFSNMQETDLIRKA
                     YMQISEFLDCKREIFDIPLSLKGTEFQKSVWTELLKIPYGKTCSYSDIAKKIKNPKAS
                     RAVGGACNKNPIMIIVPCHRVICRNGSINGYAQGVNIKSKLLNIEKTLIKT"
     gene            complement(783..1856)
                     /locus_tag="PLQ68_08075"
     CDS             complement(783..1856)
                     /locus_tag="PLQ68_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015710019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HXK72650.1"
                     /translation="MYNGLGSDLTNLTKLSDAKSRSISPENFKGEKGKGGMATEGTGA
                     NAASDLGGQGWKVSPSIVIQPGELVEIADIEGSGAIKHIWITSGGSGRDTIIRFYWDG
                     CENPSVECPIGDFFANAFLPIHIPVNSLPICNNSVRGFNCYWEMPFRKHCRITLENIG
                     PKRTYHYYQIDYTLTEIPDDCAYFFAQFRRENPIKYKTDFTILDNVEGKGHYVGTYMT
                     WGANNNGWWGEGEIKFFMDDDKNFPTICGTGTEDYFCGAYSFLHNNEYAEYSTAYCGF
                     YPVRYENEVQGQQRFSMYRWHVTDPVRFEEKLKVTMQALGWRSDGKYLALQDDVSSVA
                     YWYQTMPFNKFPELPKYEFLEII"
     gene            complement(1969..2670)
                     /locus_tag="PLQ68_08080"
     CDS             complement(1969..2670)
                     /locus_tag="PLQ68_08080"
                     /inference="COORDINATES: protein motif:HMM:NF039407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6320 domain-containing protein"
                     /protein_id="HXK72651.1"
                     /translation="MSKLPYCVKCGVELDNSANKCVLCGTEVVLSCQKDITPYPKEKA
                     EVSQLNSKFIASMLTIMLAIPNVACFVINMIYFAGVYWMYYVFGGSLVVWMIFIFPML
                     LKKKRPILHVFMIFLSATLYILLISIAVSGMDWFLILALPISIAVMFLLFIIIFIVDR
                     TKPGKLKTTSVSLTAVILLCLIVEILVDYYVTANISLSWSYVVAGSIVPVIIFLILLS
                     GNKKMQDELIKKLHI"
     assembly_gap    2699..2798
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2799..3790)
                     /locus_tag="PLQ68_08085"
     CDS             complement(<2799..3790)
                     /locus_tag="PLQ68_08085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol acetyltransferase"
                     /protein_id="HXK72652.1"
                     /translation="MLRTVDDKKQNNWFKLDNAAKIYPAANTKRWSGMFRLSVTLKEK
                     IDSFILQEALAQTIKRFPSFNIRMRSGLFWYYFETNAVKRPIVKPDVNNPCAPISWKD
                     NNGFLIRVFYYDKRIAIDYFHSLTDGTGAMIFLKTLTAQYLRLKGFDIPTTDGVLDVD
                     ELPQTDEYEDSFGKHANSKFKGRIKENKAYHFKGNKLEGDDISIITGITSLEAIKKLA
                     KSNNATITEYLSALLLYSFYIQKQKDGLYRKPTRLAVSIPVNLRNYFKSTTNRNFSLF
                     LNPGIDTIHGEYTLVEILNQIQYFMKANLNDKVLNAMMSQNVRIEKNMLIRVAP"
     gene            complement(3775..4257)
                     /locus_tag="PLQ68_08090"
     CDS             complement(3775..4257)
                     /locus_tag="PLQ68_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015925987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HXK72653.1"
                     /translation="MKIIEKWNDKLESQNAADFYNAYLETEKEVYTRILAEKMEALKG
                     TVAELSKNFGMEDWVFAGFMDGINTSLKEPADIENLEEDTLLDLTIDFEKLYYNMINV
                     KAEWLYTLKEWDNILSVDKRAQIKKQHTIDHTAVRDKVGRNDPCPCGSGKKYKKCCGQ
                     "
     gene            complement(4265..6163)
                     /gene="thrS"
                     /locus_tag="PLQ68_08095"
     CDS             complement(4265..6163)
                     /gene="thrS"
                     /locus_tag="PLQ68_08095"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015924874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HXK72654.1"
                     /translation="MIKIKLPDGTIKEYDKAVTAYEIALGISKGLAKAVVGAKVNGKI
                     RSLTDPINEDSDLLLLKFEDEGADHIYRHTSSHIMAHAVTSLFPGTLLAIGPAINNGF
                     YYDFDTEHVFTEDDFADIEKKMAEIIKQNYPIERFVLPKQEALDLMKDNPYKVELINE
                     LDESEEISFYRQGDFVDLCAGPHLPFTGSVKVIKLLSVAGAYWRGDEKNKMLQRLYAT
                     SFPSSQMLDDYIFKMEEAKKRDHRKLGRELDLYDMFEEGPGFPFFFPKGMEIRNQLID
                     YWRREHKRAGYGEILTPIILNEELWHRSGHWDHYKNNMYFTTIDEANYAIKPMNCPGG
                     MVAFKRRMYSYRDLPLRTAELGLVHRHELSGALHGLMRVRCFTQDDAHIFMTQEQMKS
                     EIIGVIKLMEKIYSVFNFEYKIELSTKPENSMGTKEQWDIAEKALEDALIALDMKFDL
                     NPGDGAFYGPKIDFHIQDSIGRTWQCGTIQLDFQMPERFDLTYTGADGNKHRPIMIHR
                     ALFGSIERFIAILTEHYAGAFPLWLSPVQVKILPLTDRQAEYAQKVHDILVENDIRVE
                     CDFRSEKIGYKIREAQMEKIPYMLILGDKEMENNQVSVRVRKIGDIGTLFLEEFMNRL
                     LKEINDKV"
     gene            complement(6509..>7063)
                     /locus_tag="PLQ68_08100"
     CDS             complement(6509..>7063)
                     /locus_tag="PLQ68_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010024696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HXK72655.1"
                     /translation="SCEIHSMSMEFFTWPWMNLFFEEQSDKYKYAHLSGALTFIPYGT
                     IVDYFQHIVYEKPQMTPDERKQVWKDLQKEFMPYLNTDGMPFFEDGRYWQRQHHIYSS
                     PFYYIDYCLAQTVALEYWAESQIDYKASWNKYIAMTKRGGTDTFTGLVKSAGLESPFE
                     EEALKKVAKIAREWLKDFDKDKLV"
     assembly_gap    7064..7163
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7164..8028)
                     /locus_tag="PLQ68_08105"
     CDS             complement(<7164..8028)
                     /locus_tag="PLQ68_08105"
                     /inference="COORDINATES: protein motif:HMM:NF013591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HXK72656.1"
                     /translation="MKFSQMPYKRVTFEEIEKKANELLKKMNDAKNVKEALDAFYEYN
                     EFSDKISTAISMSYIKFTLNTEDDFFVTEKEYYDEIMPKIQDIDLKFKLALYNSKFKD
                     DLKKELKDLLFLNIEISMKAFDSKIIPHMQEENRLVNEYDKLLASAQIEFDGKTLNLS
                     QLGKYKQNTDRDIRKAAYEAEAGFYLKNGEKLDSLYDELVKIRTTMAKELGYENFVEL
                     GYYRMMRNCYTKQDVQKFRDYVKKYIVPIAMDIKNEQKERINVDKFMLYDDPLEFLSG
                     NAVPKGEPEKIF"
     gene            complement(8025..9926)
                     /locus_tag="PLQ68_08110"
     CDS             complement(8025..9926)
                     /locus_tag="PLQ68_08110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009324717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HXK72657.1"
                     /translation="MYTKDSRIKTLFDAPLGKDILDLILYMSGKKPFLFKNPFIGNIP
                     LKKLPSLTKNLISDDFLDTLITMLNREPKKHVCDVRHETKAWWKSAVFYQIYPRSFMD
                     SNNDGIGDLQGIIQKLGYLKDLGVDALWLSPVYDSPNDDNGYDIRDYEKIMEEFGTMD
                     DFDELLEKSHKLGMKLIMDLVINHTSDEHIWFQKALKDKNSKYHNYYFFKKGKDGNPP
                     NNWTSVFKGSAWNYYEHLDEYALHVFSKKQMDLNWENEQLRHDLYCMINRWLEKGVDG
                     FRLDVINLISKTKDLPDGSKALEGLVGAIGYEHMFYGPKLHEYLKEMRKETFDKYNAF
                     TVGEGAGIGLEASKFITCEKRKELDQIFNFDQLNNPGKNRYDSYKYDLRILKQLMLKW
                     QTQFNTCCWPTIVFENHDNPRFLSRITKNEAHRNYAAKLIIMLMLTLKGTPYFYQGQE
                     IGMIDSIFSKPSDLRDVEAINMYNMYLEQGMSQQEAMDKVSFGTRDHARIPMQWEDSA
                     YGGFSKKQPWIDCSRDYMKYNVKSQLTDSNSILNFMKNLIALRKENEALVYGSFINAG
                     NDDDTFCYYRKIENQLFYIELNLIDTNKKRRIDVNNKKYNKILSNYTDNDELLRPFEA
                     NIYMIGDNK"
     gene            complement(10250..11956)
                     /locus_tag="PLQ68_08115"
     CDS             complement(10250..11956)
                     /locus_tag="PLQ68_08115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72658.1"
                     /translation="MESRKKAGITIVILIVIVITALLSVSCDRSEKLLEIFNVTTFKS
                     DIVIKRVDGQEPLNMPLKYAMYILRSRSEFEDEIVSLNISSVRYMMSNTSLSMSSSEG
                     IYAKSDSSEVKEVIDSIKYCIGITTLNGIFTDKEVSKITLYEGYTADLLEDNLQNYAI
                     IPSTLSKHIKARLSDAKKVISIKNSTTNSFENFKIIGEYATDKEHDAIYLSFGGLSRA
                     VAGVNMDVSDHIEYMEIDVDENKDLANFVYYINNIFADDNMLSQYSKRINRLNEPYPY
                     MFINTVGLEPVYIEEDADFEKNVITISRIDGKENLEMSHLYSDALVKDYFDYSRYITD
                     IVISTGRKGVNPADYPSGTNYQPYGLKLMTLGRSQDSIWMDYPLPPYHQAITSISEIK
                     SDKKNSEIYFYGTYANKDLVVQREEDYRNRATQRGSAMEGYAIVPAPMFEAIRHYVST
                     DQQILELCATDESNPNNLLYVAFTAIGYYELPEDSTDQYDVIYITYVGNNNKYKKEAY
                     KNEYIESITIETNCDADMESLTRYLRQYFAPSDVAGEYTGLKNELGLNYEYCYTIKEN
                     VD"
     gene            12304..12936
                     /locus_tag="PLQ68_08120"
     CDS             12304..12936
                     /locus_tag="PLQ68_08120"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HXK72659.1"
                     /translation="MLLKENMKILMIGDSVTDCNRRSETTAFLGDAYPHLVASELYLN
                     YPDLNVEVTNMGISGDTSRMLLDRWDKDVLKHSVDVLTILIGINDVWRHYDSINRAVW
                     VSKDEYIKNMTYMIESMINRTKKIIILSPFFLETDTTQPMKAQADEYIQALRDMVKKY
                     DVIFVDLQKEFEKLMEKFTYNQLTFKTDKIHPTMFGQYVIASKILKALKS"
     gene            complement(12933..>13067)
                     /locus_tag="PLQ68_08125"
     CDS             complement(12933..>13067)
                     /locus_tag="PLQ68_08125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005384028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HXK72660.1"
                     /translation="VSSGAALCACVELAKKVENIGKNIVCILPDTGERYLSTPLYMQD
                     "
BASE COUNT         3899 a         2485 c         1812 g         4671 t
ORIGIN      
        1 ctcttaatac aaacgtctag tgcttcattg ttataaggct ttgatattcg tctacggtag
       61 catcatttag tagcagctta attatctcat gaccaagagg ttttaatgaa ggagtatcaa
      121 actttataat ttccttttta aaaaagtctg ttaaaatttt tgaaccttca tcataccctt
      181 catttgcaac ctcaacttgt aaatgtggtt gcaaaagaac ctttctgata tattgtccat
      241 ctactttaag tgaatctaat gaatatccca gtaaagaaca tcatgaatat actaaatacc
      301 aagagaaaat tttattcatg tttttattaa tgttttttct atgtttagta atttactttt
      361 tatatttact ccttgagcgt acccatttat agaaccattt ctgcaaataa ccctgtgaca
      421 tggcactatt atcattattg ggttcttgtt acaagcaccc cccactgctc tagaagcttt
      481 ggggttttta attttttttg caatatcaga gtaactacat gtctttccgt atggtatctt
      541 tagcaattct gtccaaacgg atttttgaaa ttcagtccct ttcaatgaga gcggaatatc
      601 aaaaatttct cgtttacagt ctagaaattc gcttatttgc atgtatgctt ttctgattag
      661 gtctgtttct tgcatattac taaaatcatt acatgacatg cttatacaag atatcccaat
      721 atcgtcctct gtaatgcata ttctgccaat tttcgtatca tagcagtaac tgttcactgt
      781 tactatatta tctctaaaaa ttcatatttt ggtaattcag ggaatttatt aaatggcata
      841 gtctgatacc agtatgcgac tgaagataca tcatcttgca atgctaaata tttgccgtct
      901 gatctccacc ccaatgcctg catagtaact tttagttttt cttcaaatct tacaggatcg
      961 gtaacatgcc atctgtacat tgagaatctt tgctgtccct gaacttcatt ttcatatctc
     1021 acaggataaa aaccgcaata tgctgttgaa tactctgcat attcattatt gtgcaaaaaa
     1081 ctgtatgctc cacagaaata gtcttcggta cctgttccgc atatagtagg aaaattctta
     1141 tcgtcatcca taaagaactt gatttctcct tctccccacc atccgttatt attagcaccc
     1201 caagtcatgt aagttcctac ataatgtcct ttaccttcaa cattgtctaa aatggtaaaa
     1261 tctgttttat actttattgg attttctctt ctgaattgag caaagaaata tgcgcagtcg
     1321 tcaggtattt ctgtcaaagt atagtctatc tgatagtagt gatatgttct tttaggacct
     1381 atgttttcca atgttatcct gcagtgcttt ctaaaaggca tttcccaata acaattaaat
     1441 ccccttacgc tattattaca tattggcaat gaattgacag gaatatgtat aggaagaaaa
     1501 gcatttgcga agaaatctcc gattggacat tctacagaag gattctcaca accatcccag
     1561 taaaacctta ttattgtatc ccttcctgag cctccacttg taatccatat atgtttaatt
     1621 gctccagaac cttctatatc agctatttca accagctctc caggttgtat aactattgaa
     1681 ggagaaacct tccaaccctg gcctcctaaa tcagaagcag catttgctcc tgtaccttca
     1741 gtagccattc cgccttttcc tttttcacct ttaaaatttt ctggacttat ggatcttgat
     1801 tttgcatctg ataatttcgt taagtttgtt aaatcgcttc ctaatccgtt atacataact
     1861 acctccaata atgcatgaaa tataaactga ataaaaattc gtatatgaat ttgtatatca
     1921 gtatatatag agtataagca tatatgttaa tagatatcaa tagctaaatt aaatatgaag
     1981 tttttttata agctcatctt gcattttttt gttacctgaa agcagtataa gaaaaattat
     2041 cacaggcaca atggatcctg caaccacata tgaccatgaa aggcttatgt ttgctgtaac
     2101 ataataatca accagaattt caacaattaa gcaaagcaat ataactgcag tcagggaaac
     2161 ggaagtagtt ttaagttttc ctggttttgt tctatccact ataaaaataa tgataaaaag
     2221 taaaaacata acagcaatgc tgattggtaa tgcgagtatc aaaaaccaat ccataccgga
     2281 taccgctatg gaaataagca agatatacaa tgttgctgat aaaaatatca taaatacatg
     2341 caagattggt ctcttttttt ttagcaacat aggaaaaatg aatatcatcc atacaaccag
     2401 actgcctccg aatacataat acatccagta aacaccagca aaatatatca tgtttataac
     2461 aaaacatgct acatttggaa ttgcaagcat tattgtaagc atagatgcta taaactttga
     2521 gttcagttgg gacacttcag ctttttcctt gggatatggt gttatatctt tttgacaaga
     2581 tagtactact tctgtaccac atagcacaca cttgtttgca ctgttatcca attctacacc
     2641 acattttacg caatatggca atttgctcac ctactctctg ttactttcaa ttttaacgnn
     2701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnaa aggtgctact cttattagca
     2821 tatttttttc aatacgaaca ttctgagaca tcatagcatt taaaacttta tcatttaaat
     2881 tcgctttcat aaaatattgt atctgattaa gtatctctac aagtgtatat tccccgtgaa
     2941 ttgtatctat accgggattg agaaataatg aaaaattcct gtttgttgtg cttttaaaat
     3001 aattcctcaa atttactggt atagaaactg ctaacctggt tggctttcta taaagaccat
     3061 ctttctgttt ttgaatataa aatgaataaa gaagcaatgc agataggtat tcagtaattg
     3121 tcgcattatt gctttttgct aatttcttaa ttgcttccaa tgacgtaata ccagtaataa
     3181 tagaaatatc atcaccttca agcttattcc ccttaaaatg atatgcttta ttctctttta
     3241 ttcttccttt gaattttgaa ttcgcatgtt tgccaaaaga atcttcatac tcatcagtct
     3301 gaggaagttc atcaacatca aggactccat cagtcgttgg tatatcaaaa ccttttaacc
     3361 gtaaatactg tgcagtcaat gttttaagaa aaatcatagc tcctgtacca tctgttaggg
     3421 aatgaaagta atctattgct attcttttat cataataaaa aacccttatt aaaaacccat
     3481 tattatcttt ccagcttata ggtgcgcaag gattatttac atcaggtttt acaataggtc
     3541 tttttacagc atttgtttca aaataatacc agaaaagccc gcttctcatt ctaatattga
     3601 agcttgggaa tctttttatt gtctgagcca atgcttcctg aagaataaaa gagtcaattt
     3661 tttccttcag ggtaacactg agcctgaaca taccagacca tctttttgta tttgcagcag
     3721 gataaatctt tgcagcatta tccagtttga accagttatt ctgtttttta tcgtctactg
     3781 tccgcaacat ttcttgtatt tcttaccgct tccacatgga catggatcat ttcgaccaac
     3841 tttatccctt actgcagtat gatcaattgt atgctgtttt ttaatctgag ctcttttatc
     3901 aacagataaa atattgtccc attctttcag ggtgtatagc cattcagctt ttacattaat
     3961 catattataa taaagcttct cgaaatcgat tgtcagatcc agtaaagtat cttcctcaag
     4021 attctcaata tctgcaggtt ccttaagact ggtattaatc ccatccataa atccggcaaa
     4081 aacccagtct tccataccga aattttttga aagctctgcg actgtacctt ttagtgcttc
     4141 cattttctct gcaagtatac gtgtatatac ctctttttct gtttcaagat atgcattata
     4201 aaaatcagct gcattctgtg attctagctt atcattccat ttttcaataa tcttcatatt
     4261 tcacctacac cttgtcatta atttccttaa gaagcctgtt catgaattct tcaaggaata
     4321 atgtgccaat atctcctatc tttctaactc tgactgatac ctggttattt tccatttcct
     4381 tatctcccaa tatcagcatg tatggtatct tctccatctg agcttctctt atcttataac
     4441 cgattttttc agatctgaaa tcgcattcaa ctcttatatc gttctcaaca agtatatcat
     4501 gaactttctg tgcatactcg gcttgtctgt cagttaaagg aagaatctta acctgcacag
     4561 gtgaaagcca taatggaaaa gctcctgcat aatgttcagt aagaattgca ataaatcttt
     4621 caatagaacc aaaaagagct ctatgaatca taataggcct gtgtttgttg ccatcagctc
     4681 ctgtatatgt aagatcaaat ctttccggca tttgaaaatc aagctgtata gttccgcatt
     4741 gccatgtacg tcctatggag tcctgtatat gaaaatcaat ctttggacca taaaatgctc
     4801 catcaccagg gttaagatca aacttcatat ctaatgctat aagagcatcc tcaagagctt
     4861 tttcagctat atcccactgt tctttagtac ccatcgaatt ttccggtttt gttgaaagtt
     4921 caatcttgta ctcgaaatta aaaacgctat atatcttctc catcagttta attacgccaa
     4981 taatttcgct cttcatctgc tcttgtgtca taaatatatg agcatcatcc tgtgtaaagc
     5041 atcttactct cattaaacca tgtaatgctc cggataattc atgtctgtga accagtccaa
     5101 gttcagctgt tcttaatgga agatccctat acgaatacat acggcgttta aatgctacca
     5161 ttccaccagg acagttcata ggtttaattg cataattagc ttcatctatc gttgtaaaat
     5221 acatattatt tttataatga tcccagtgtc ctgaacgatg ccataattct tcattaagta
     5281 ttataggtgt taaaatttct ccataccctg ctcttttgtg ttctcttctc cagtaatcta
     5341 ttaactgatt gcgaatttcc attccttttg gaaaaaagaa aggaaatcca ggaccttctt
     5401 caaacatatc ataaagatca agttctctgc caagctttct atgatctctt tttttggctt
     5461 cttccatttt aaatatgtaa tcatccagca tctgagatga aggaaatgat gtagcataaa
     5521 gtctttgaag cattttgttc ttttcatcac ctctccaata tgctcctgca actgataaaa
     5581 gtttgattac cttaactgaa ccagtaaatg gaagatgggg ccctgcacat aaatcaacaa
     5641 aatcgccttg tctgtaaaat gaaatttctt cagactcatc caattcattt ataagttcta
     5701 ccttatatgg gttatctttc attaggtcca atgcttcttg ttttggtaat acgaatctct
     5761 caataggata attctgttta ataatttctg ccatcttctt ttctatatct gcaaaatcat
     5821 cttctgtaaa aacatgctct gtatcaaaat cataatagaa gccgttattt atggcaggac
     5881 ctatagccag taatgtgcca ggaaatagag aagtaacagc atgagccatt atatgactgg
     5941 atgtatgtct gtatatatga tcagcacctt catcttcaaa tttcagaagg agcaaatcag
     6001 aatcctcgtt tatcggatct gtaaggcttc ttattttccc attaactttt gcgcctacta
     6061 cagcctttgc caaaccttta gaaattccta aagctatttc ataagctgtt acggctttat
     6121 catattcttt tattgtgcca tccggcaatt taatcttaat catttaaaca cctcctataa
     6181 gttccttaat tataaaaaaa actgccctga atcattagtt caggggcagt ataatttccg
     6241 cggttccacc ctgattagcc atctggctat ctcaagcaat cgtatcgtga tctgacgatt
     6301 ctgttcaagg agcggttttc agcgtgctgt gaaaggttct cagctaagcc cttctctctg
     6361 ttcattctcg cttactcttt tcctatcatt acagcaatat aatgatttgc gccaatttta
     6421 ccacttgttt ttgcaatatg caagtttttt ttgattattg aagcaaatta atagtggata
     6481 atcaatgagt ccgcaagttt atttgttctt acactaactt atccttatca aaatccttta
     6541 accattctcg tgctattttg gcaactttct taagcgcttc ttcttcgaat ggactttcca
     6601 gtcctgcaga tttaactaat cctgtaaatg tatccgttcc tcctcttttt gtcattgcta
     6661 tatatttatt ccatgatgct ttgtaatcaa tctgcgattc tgcccaatat tctagtgcta
     6721 ctgtctgtgc taagcagtaa tcgatataat agaaaggact tgaataaatg tgatgttgtc
     6781 tctgccagta tcttccatct tcaaaaaatg gcattccatc agtattaaga tatggcatga
     6841 attctttttg cagatccttc catacctgtt ttctttcatc tggagtcatc tgaggttttt
     6901 cgtatacaat atgctgaaag taatcaacaa tagttccata tggaataaat gttaatgctc
     6961 cggataaatg tgcatactta tatttatcag attgttcttc aaagaaaaga ttcatccatg
     7021 gccatgtaaa aaattccatt gacattgagt gtatctcaca agannnnnnn nnnnnnnnnn
     7081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7141 nnnnnnnnnn nnnnnnnnnn nnnaaaatat cttctcaggt tctcctttag gaactgcatt
     7201 acctgacaag aattcaagcg gatcatcata aagcatgaac ttatctacat taattctttc
     7261 tttttgctca ttttttatat ccattgcaat cggaactatg tattttttta cataatcacg
     7321 gaatttctga acatcctgtt ttgtataaca gtttctcatc attcggtagt atcctaactc
     7381 gacaaaattc tcatatccca gttctttagc catagttgtt cttatcttta ctaattcgtc
     7441 ataaagacta tctagctttt caccgttttt aagatagaat cccgcttccg cttcatatgc
     7501 cgcttttctt atgtctctgt cagtattctg tttatattta cccaattgag acaaattcaa
     7561 agttttacca tcaaattcaa tctgtgcact tgcaagaagt ttatcatatt cattaactaa
     7621 tcgattctct tcctgcatgt gcggaataat tttcgaatca aaagctttca tgctaatttc
     7681 aatattcaaa aacagtaaat ctttcagttc ttttttcaaa tcatctttaa attttgaatt
     7741 atacaaagca agtttgaatt tcaagtcaat atcctgaatt ttaggcatta tttcatcata
     7801 atattctttt tctgttacaa aaaagtcatc ttctgtattc aatgtaaatt ttatgtatga
     7861 catagaaatc gcagtactta tcttatctga aaattcgttg tactcataaa aagcatcaag
     7921 tgcctccttt acattttttg catcattcat tttctttaaa agttcattag cttttttttc
     7981 aatttcctcg aatgttactc gtttgtaggg catttgtgaa aatttcattt attatctcca
     8041 atcatatata tatttgcctc aaaaggcctt aataattcgt cattatctgt ataattcgat
     8101 aatatcttat tatatttttt gttgtttaca tcaattcgcc gtttcttgtt tgtatctata
     8161 aggttcagtt caatgtaaaa gagctggttt tctattttac ggtaatagca gaatgtatcg
     8221 tcatcatttc cagcattgat aaagcttccg tataccaaag cttcattttc ttttcttaat
     8281 gcaataagat ttttcataaa attcaaaatt gaattagaat cagttagctg acttttaaca
     8341 ttatacttca tatagtctct tgaacaatct atccatggtt gtttttttga gaagcccccg
     8401 tatgcgctat cttcccattg catgggaatt cttgcatggt ctcttgtgcc aaaacttact
     8461 ttatccatcg cttcttgctg actcattcct tgttctaaat acatattata catattaata
     8521 gcttctacat ctcttaaatc agatggttta ctgaaaatag aatcaatcat ccctatttcc
     8581 tgtccctgat aaaagtatgg agtccctttt agtgtaagca ttagcataat aatgagtttt
     8641 gcagcataat tcctatgagc ttcattttta gttattcttg ataagaatct cggattatca
     8701 tggttttcaa atacaattgt aggccagcag catgtgttga actgtgtctg ccatttgagc
     8761 attagctgtt ttaatattcg taaatcatat ttatatgaat catatctgtt tttccctgga
     8821 ttattcaact ggtcaaagtt gaatatctgg tcaagttctt ttcttttttc acaagttata
     8881 aactttgagg cttccaagcc aattcctgca ccctcaccga ctgtaaaggc attgtattta
     8941 tcaaaggtct ctttacgcat ttcttttaga tattcatgca atttcggacc ataaaacata
     9001 tgctcatatc ctattgctcc tactagtcct tcaagtgctt ttgaaccatc tggtagatct
     9061 tttgtcttgg aaatgagatt aattacatca agtctgaaac cgtcaacacc tttttccaac
     9121 caacgattta tcatacagta aagatcatgt cttaactgtt cattttccca attaagatcc
     9181 atctgttttt tcgaaaatac atgcaaagca tattcatcaa gatgttcgta ataattccat
     9241 gctgatcctt taaaaacaga tgtccagtta ttaggaggat taccatcttt cccttttttg
     9301 aagaaatagt aattatgata ttttgagttt ttgtctttta acgccttttg aaaccaaata
     9361 tgctcatctg aagtatgatt aatgacaaga tccattatta acttcattcc caatttatgc
     9421 gatttttcaa gaagctcgtc aaaatcatcc atcgtaccga attcttccat gattttttca
     9481 taatcacgaa tatcataacc attgtcatca tttggtgaat catatacagg tgaaagccat
     9541 aaagcatcta ctcccagatc ttttaaatag ccaagcttct gtattatacc ctgcaaatcc
     9601 cctattccat cattattgga atccataaaa gaccttggat atatctgata gaatactgcg
     9661 cttttccacc aggctttggt ttcgtgtctg acatcacaaa catgcttttt tggttctctg
     9721 ttcaacattg tgataagtgt atctagaaag tcatcagata ttagattttt ggttaatgat
     9781 ggaagctttt taagtggtat gttcccaata aaaggattct taaacagaaa tggtttttta
     9841 cctgacatat acagaatcaa atccagtatg tctttaccta aaggcgcatc aaatagcgtt
     9901 tttattctgc tatctttcgt gtacatttca gctctccttc tttattacaa ttctatcatt
     9961 taataatata tgaatcaaac ataaaaatat attttatctg gaaatttgat taagtgatat
    10021 aattgataaa taaactttta tttggaggca tatatgctat taaaagaaaa catgaagatt
    10081 cttatgatcg gagactcggt cactgattgc aatataagaa gcaaactgcc ttcgtaataa
    10141 ctgaagtgtc tcgctaaaaa tcttggtttt acattcaaat caaagggtaa taatcttata
    10201 acgattttga tttattaatt gtgatataaa catcaggtat agtttgaaac taatcaacat
    10261 tctcctttat tgtataacag tactcataat ttaaacccaa ttcatttttt aaccctgtat
    10321 attcacctgc aacatccgaa ggtgcaaaat actgccttaa atatcttgtc aaagattcca
    10381 tatcagcatc acaatttgtc tctatagtta tagactcaat gtattcgttt ttatatgcct
    10441 cttttttata cttattatta ttacccacat atgttatata tattacatca tactgatctg
    10501 ttgaatcttc aggcaactcg taataaccta ttgccgtaaa tgcaacatat aacaaattat
    10561 tagggttgct ttcatctgtt gcacataact ctaaaatttg ttggtctgtt gatacataat
    10621 gtcgaatagc ttcaaacatt ggtgcaggaa caatagcata tccttccatg gcactccctc
    10681 tttgtgtagc acgatttctg taatcttcct ctctttgtac aacaaggtct ttgtttgcgt
    10741 aagtaccata aaaatatatc tcactgttct ttttatcgct tttaatttca cttatacttg
    10801 taatagcctg atgataaggc ggtagcggat aatccatcca gatactgtct tgcgaccttc
    10861 ctaaagtcat cagctttaaa ccataaggct gataattcgt ccccgaaggg taatccgccg
    10921 gattcacccc ttttctaccc gtacttatta ctatgtcggt tatgtaccta gaataatcga
    10981 agtaatcttt tactaaagca tcgctatata aatgcgacat ctccagattt tctttcccgt
    11041 ctatcctaga aatggttatt acattctttt caaaatctgc gtcttcctca atatataccg
    11101 gctctaatcc cacagtgttt ataaacatat atggataggg ttcattaagc ctgtttatcc
    11161 tctttgaata ttgagaaagc atattatcgt ctgcgaatat attgtttatg taatatacga
    11221 aattggcaag atctttgttt tcatctacat caatctccat gtattcaata tgatctgata
    11281 catccatatt aactcctgct actgctctac taagtccacc aaaggacaga tatatagcgt
    11341 catgttcttt gtctgttgca tattccccta ttatcttgaa attttcaaaa ctgtttgtgg
    11401 tggaattttt aatggaaata acctttttag cgtcagataa cctagcttta atatgcttac
    11461 tcaaggtaga gggaataata gcgtaattct gaagattatc ttctagcaaa tccgcagtat
    11521 agccttcata taatgtaatc ttactaactt ctttatctgt aaatataccg tttagtgtag
    11581 ttatacctat acaatacttt atcgaatcaa taacttcctt aacttcacta ctgtccgatt
    11641 ttgcataaat gccttcagaa ctagacatgc ttaagcttgt attgctcatc atatatctta
    11701 ccgatgaaat atttagactg actatttcat cttcaaattc cgatctgctt ctcaatatat
    11761 acatagcata tttcaatggc atatttaacg gctcttgacc gtccactctt tttataacta
    11821 tgtcactttt aaaggtcgtt acattaaaaa tctccaacag tttttccgat ctgtcacaag
    11881 acactgacaa caatgctgtt ataacaatca ctattaaaat aactatagtt atacctgctt
    11941 ttttcctgct ttccatgctg tttgtttatc cattcaccat tgatatgatt ggattctcct
    12001 ttctctcgga cttttctgag taattcctta ccttttatgg catatttaaa ttaagaactg
    12061 tggacttgta cccatactgc ctgttttttg ttttgcattc aaatacagaa ttaatccagt
    12121 atgtctttac ctaaaggtgc atcaaatagc attttctttc tgctatcttt catatacatt
    12181 tcagttcttc tttattacaa ttctatcatt taataatata tgaatcaaac ataaaaatat
    12241 attttatctg gaaatttgat taagtgatat aattgataaa taaactttta tttggaggca
    12301 tatatgctat taaaagaaaa catgaagatt cttatgatcg gagactcggt cactgattgc
    12361 aacagaagaa gtgaaactac agcattcctt ggtgatgcat accctcacct tgtagcatcg
    12421 gaactttatc tgaattatcc tgatttaaat gtggaagtaa ctaatatggg tatttccgga
    12481 gacacttcca gaatgctttt ggacagatgg gataaggatg tattaaaaca ttctgttgat
    12541 gttcttacca tattaatagg tataaatgat gtatggagac attatgattc tattaatcgt
    12601 gctgtatggg tatcaaaaga tgaatacata aaaaatatga catacatgat tgaaagcatg
    12661 ataaatcgca caaaaaagat tataattcta tcacctttct tcttagaaac tgacactaca
    12721 caaccaatga aggctcaggc tgatgagtat atacaagctc ttcgtgatat ggttaagaaa
    12781 tatgatgtaa tctttgttga ccttcaaaaa gaatttgaaa aacttatgga aaaattcaca
    12841 tataatcagc ttacctttaa aacagataaa atacatccga caatgttcgg tcaatatgtt
    12901 attgcaagta agatactgaa agccttaaaa agttaatctt gcatatataa aggggttgat
    12961 aaatatcttt caccggtatc gggaagaatg caaactatgt tcttccctat attttcaacc
    13021 ttttttgcta actcaacaca agcacacaga gcggctcctg aagatac
//
LOCUS       DAOSXM010000063        12324 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2111
            NODE_3262_length_12324_cov_11.835031, whole genome shotgun
            sequence.
ACCESSION   DAOSXM010000063 DAOSXM010000000
VERSION     DAOSXM010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864178
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 12324)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12324)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:24:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,261
            CDSs (total)                      :: 2,229
            Genes (coding)                    :: 2,218
            CDSs (with protein)               :: 2,218
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 3 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12324
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3262_length_12324_cov_11.835031"
                     /isolate="AMR_MDS_2111"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(127..810)
                     /locus_tag="PLQ68_08130"
     CDS             complement(127..810)
                     /locus_tag="PLQ68_08130"
                     /inference="COORDINATES: protein motif:HMM:NF019559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HXK72661.1"
                     /translation="MTGIFISLAIFFIMLCVYIYHIIFKEEPHEKITKTAPSVMFFVT
                     AVVSVILTGNYTTTALFLCAGLFLSIFGDFFLIKKIKIKRSFIIGVAFFFTAQICYII
                     SFSILVPITWFDIFLFIGLEGSALLVYKLMRFDAKNLKIPLFLYSCALSFMAVKAVSL
                     VFARPFGIYHGITVAMGGLLFLFSDCVLSHSILTGGRTLRARLINLVTYYFGQIMLAF
                     SIYLVFMNY"
     gene            941..1480
                     /locus_tag="PLQ68_08135"
     CDS             941..1480
                     /locus_tag="PLQ68_08135"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HXK72662.1"
                     /translation="MDILEKITDRKIMYTGKIIEVEKVTAILPNGKEVYRDVVRHNGA
                     CGIVALDEDMNIVLEKQYRIAFDDILIELPAGKLDKDEEPLQCAIRELEEETGYVADS
                     MTHILTVASSPGFSDETVHLFLAEGLTQKEMKWDEDERLIVWKEPLIKAKQMVLEGKI
                     KNGLAVMGILAACQIKGIN"
     gene            1485..2276
                     /locus_tag="PLQ68_08140"
     CDS             1485..2276
                     /locus_tag="PLQ68_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXK72663.1"
                     /translation="MIKFCPLFSGSSGNALYLKYNDTSILIDAGLSKKRITQAISDIG
                     EDASKLNAILITHEHIDHTSGIGVMERGYNIPVYANISTWKNMPSFLGKIREDNKKTF
                     ETGKKFTINDIEILPFEIPHDAADPVGYSFYLDSRKVTSVTDIGHLTDTILENIKGSD
                     LLLMESNHDLDMLIKGPYPWPLKNRVKGNFGHLCNKDCGDLIARYIEFGLKTIVLGHL
                     SAENNTPQLAYDTIKQCIESFGYEIGRDIDLDVAKREGCNRVFEL"
     gene            2273..2731
                     /gene="rlmH"
                     /locus_tag="PLQ68_08145"
     CDS             2273..2731
                     /gene="rlmH"
                     /locus_tag="PLQ68_08145"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HXK72664.1"
                     /translation="MKINIICVGKLKEKYLAEAQAEYIKRLSRFCDVSILELNDEKDD
                     IPDAIEKEGTKILSKIDTKDYVICLAIEGKKLNSEAFSEKIISVFDSAKPVIDFVIGG
                     SLGLSDNVKKRADMLLSFSDMTFPHQLMRIILLEQVYRAFKIANNENYHK"
     gene            2747..3355
                     /locus_tag="PLQ68_08150"
     CDS             2747..3355
                     /locus_tag="PLQ68_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012059351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HXK72665.1"
                     /translation="MKNIITIQYTKSIHHTNGMVGSWTDWDLSEFGISQADKIGENLK
                     KEISDKKYVMYSSDLMRAKHTAMIVADYLGITPIYTEVLRERNLGQAVGESVEWLRNN
                     MTEEKTIDDRMFYDAETIHEQYDNLLKFHEQIMKSDNENILIVSHGGTLSVFHAIWLG
                     LDADMLNRCNLYGKAGGVSFMDIDIRGSKRITRLSDMSYSLI"
     gene            3414..4004
                     /locus_tag="PLQ68_08155"
     CDS             3414..4004
                     /locus_tag="PLQ68_08155"
                     /inference="COORDINATES: protein motif:HMM:NF016085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HXK72666.1"
                     /translation="MKRKRLDRDIWTFDKYPYYQLRVDMEDFHGLVCFIQLKGGVAHI
                     NGGQYQYWDRPKAGKVAICGKGMIWMQLIPDDKSHVLTVKYLPDNTVSIWYADIVENI
                     EYDSDGVAVFVDKYLDVTFTPQGDVSIDDRDELDAAFQSGELSEQQYAAAIEEGDAVV
                     KEYCSDLVKTENICYKILFYVLEKIKNGEKQFKHIV"
     gene            4382..4654
                     /locus_tag="PLQ68_08160"
     CDS             4382..4654
                     /locus_tag="PLQ68_08160"
                     /inference="COORDINATES: protein motif:HMM:NF024212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HXK72667.1"
                     /translation="MPFRYIDVNPIDKVVDNVVDNVVDKIEGLNETQCKILLAIRNNS
                     NITQSQLSVAVGIGKTAIQNNIVVLKKRGYIERVGSNKTGYWKVNE"
     gene            4636..4851
                     /locus_tag="PLQ68_08165"
     CDS             4636..4851
                     /locus_tag="PLQ68_08165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72668.1"
                     /translation="MESKRIKRRTVFLVEYTPVPENVPDYETLILFKKKLSELYLIGM
                     GVTKEQIQDYLDLIIIARKAECPNEYL"
     gene            5033..5218
                     /locus_tag="PLQ68_08170"
                     /pseudo
     CDS             5033..5218
                     /locus_tag="PLQ68_08170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251250.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
     gene            5493..5834
                     /locus_tag="PLQ68_08175"
     CDS             5493..5834
                     /locus_tag="PLQ68_08175"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007288731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HXK72669.1"
                     /translation="MYKQGEILLISLPFSDLTSSKKRPVLVISNDENNRTSEDIVVAA
                     ITSNICKRKYSVMISSSNLTEGTLKRDSCLRADKIFSLSQNIVIRKFGKVEQNVINQV
                     INCITDLITNK"
     gene            complement(5858..6937)
                     /locus_tag="PLQ68_08180"
     CDS             complement(5858..6937)
                     /locus_tag="PLQ68_08180"
                     /inference="COORDINATES: protein motif:HMM:NF016060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HXK72670.1"
                     /translation="MTFKDNGLKITVVLPSLKPTQKLVSTVTGLIEEGFSNIIVVNDG
                     SSSDYDKVFSEVSSLEQCTLLKHDVNKGKGAALKTAFQYIQQQDKDILGVVTADADGQ
                     HLPKDILKCSQQMTDDETVILGARNFYKSHVPLKSFLGNKITSFVFKTGCGININDTQ
                     TGLRVFPVKHISFLLSVKGERFEYETNMLLEMKPECIPFREVEITTVYEDKNVGSHFN
                     AVRDAFRIYKLIFKFMMSSIISFFIDNIIFFISLLIFASVLEDTRILISTVIARAVSS
                     FFNFNFNRNSVFKHKCDYRKCLLRYYILCISLMLISAGIVTVLSNVFPAEKELMITLI
                     KIGVEAVLFFISFNIQRDWVFKKCK"
     gene            complement(6955..8364)
                     /locus_tag="PLQ68_08185"
     CDS             complement(6955..8364)
                     /locus_tag="PLQ68_08185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72671.1"
                     /translation="MEKKAVIYNRKTKKQKKKFIKGLAIYSACFIVVILIGLALFWQY
                     MKSYELSRPENFMNEFVKTVDDNTIRTMMTENEKIEASEFEDINTIYDQLFFSNLKNE
                     EYKYRKMPGQYTDTLPVYIIYTDSADLCKVVLTSKGDNTAKFGFNLWQADTFTVLDSV
                     LNPEQKTLSITVPKLADVYVNDIEVSDSYVTDSSVAYSGYEDVLQPFDERPTGKKYEI
                     PGIYLEADILVKDHKGIILELNRAGNEYVYPLVIKETLSYNITAPKEAKVYINGILLT
                     EEYIENSEEYYAGFDNLVTYTQIPPLNRYLVENLYSEPNIECYGEDGLTLEKIEASDK
                     KNIIYHYSESEKLKADYEQYALGFAKVYINFTTNATGDIWGYFPTLNAYLIQDSVLQT
                     RLKSAIPSLYWVMGSTLEYNYLSVTDFRPISETCFTCQMSFSATQKTYYETKTLEDSF
                     ELVFMLYNNKWRVADMLSE"
     gene            complement(8368..9411)
                     /locus_tag="PLQ68_08190"
     CDS             complement(8368..9411)
                     /locus_tag="PLQ68_08190"
                     /inference="COORDINATES: protein motif:HMM:NF021507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HXK72672.1"
                     /translation="MTDKRSIVKKATSAILKALVVFLTTLALIVVALLGVIYVLVKGP
                     SPTAQKLFVLSVRETSAIGFLANIYLSDEELAKLTVSEIVKEPSEKTDTSLIEIPTPT
                     PSTQPNDSETDIPEITEEEKDIEVVRISGSLYEGVMLIVKDPLRLFVGTPDELGGKGL
                     KLDEMVEKYDAIAGINAGGFYDPFGGGHEGTPDGMVICNGEVVWGEPNIKLNVAGFDD
                     TGILHVGMMTTEEAMDTNIQWAVSFGPALIINGKVQSFESDLISGLNPRTAIGQRIDG
                     AVLMLVVDGRRMNSLGATYGDLADIMLDFGAVNASNLDGGSSTLMIYEGEVMNVCASV
                     TGPRALPTTFLVR"
     gene            9531..10850
                     /locus_tag="PLQ68_08195"
     CDS             9531..10850
                     /locus_tag="PLQ68_08195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HXK72673.1"
                     /translation="MHKKTFKFPILIMIAVTMFMLSGCNKPVITPSETNKPSPSISAI
                     PETETPSETPQQTPEQTPIQSEEPVEPTEEIKPDAEDITKKVYIDIDGHYSEKLSDDN
                     HYTKYTLNKGSVINISASEQIHSLYIVWDRIPGEWTLIANDEKVTGGKNGFIHEYIEL
                     SNSSKRASIELTNNSAIICDVYVFTYGNLPKWVQTWDMPYEDADMLLLSTHADDEHLY
                     FGGMMPYYGGELGYKVQVAYLVNHWNEPYRPHELLNGLWTVGMTAYPIIGEFDDLYSP
                     SLEHAKTIYPLEDVLDYQVELLRRFKPEVVIGHDLKGEYGHGAHMLNAYGLTLAVEYA
                     ADDTKYISSYEKYGLWDTPKLYLHLFEDNKILMNWDMPLEKFNGFTAFEMAVKGYDCH
                     KSQHIWSFAVRQGESQYDCRWFGLYRSLVGPDIQKNDVFENIVFEDK"
     gene            11194..12036
                     /locus_tag="PLQ68_08200"
     CDS             11194..12036
                     /locus_tag="PLQ68_08200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72674.1"
                     /translation="MEKDKVKGRIAVLITAGILIVAGAVLLFILLGNKEEGPSVYEIP
                     VQRINGTEGASLDMPYEYMRSMMNSKESLLNNAEDIEAQTIIAKRSDGIKDGIRVGYV
                     LTEKWYNGPVDRLLYVQGITSIKTIERKDNCKVTFIDGYDSSNLMKKNDGEVGGYAIL
                     PEWMKDNIAPEYKVDNISILLMKEEPKPVLRKFKVIGFYKADTEDTVYASMYSYAELY
                     KFEEDVLDSLIMSLSIYYEDGANMTSLYSFMNDYFSDSQTKKEGENLVNMDYDFFYIP
                     ITEQ"
     gene            12069..>12324
                     /locus_tag="PLQ68_08205"
     CDS             12069..>12324
                     /locus_tag="PLQ68_08205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72675.1"
                     /translation="MRYALKRLCRTPVKTIIFILLISLSVSALSISAGMWKYSYDSIK
                     HSKDAFTTIGILREIEFTEQIYKNSVKPKYDYELLSKVKQA"
BASE COUNT         4284 a         1956 c         2328 g         3756 t
ORIGIN      
        1 ttgagatttt attctgtttg ccatgtaaga atggaattta ctttttcaat ctaccttgca
       61 tatacaatga ttattgtcta catatttata atatggttct ttacagatta tcttcagata
      121 tggaaatcag tagttcatga atacaaggta aatgctgaat gctaacatta tctgcccgaa
      181 atagtaggtt acaaggttaa taagacgtgc tcttaatgtt ctgcctccag ttagtataga
      241 atgggacaat acacagtcgg aaaaaagaaa cagtaaacct cccattgcga cagtaatacc
      301 atgataaatt ccgaaaggtc tggcaaatac taaagatact gcttttacgg ccataaaaga
      361 taaagcgcag ctatatagaa atagaggtat tttaagattc tttgcatcaa acctcataag
      421 cttatataca agaagggctg aaccttcaag cccaataaag agaaatatat caaaccaggt
      481 aatcgggact aatatagaaa aggaaataat atagcaaatc tgagctgtaa aaaagaaagc
      541 aactcctatg atgaagcttc tttttatctt tatttttttt ataaggaaaa agtctccaaa
      601 tatggacaag aaaagacctg cacataagaa taacgcagtt gtggtatagt tgccggtaag
      661 tataactgat acaacagctg ttacaaaaaa cataacgctt ggagcagttt tagttatttt
      721 ctcatggggt tcttctttga aaatgatgtg gtatatatac acgcacaaca taatgaaaaa
      781 tatcgccaga cttataaata tacccgtcat attaacctct gttatattaa ttttaaccta
      841 tagggtaagc ataagcaaac acatattgaa ctaattgatg tatacaaaca tgtattaatc
      901 tgtttcagat ggtataatat attgaaacaa aggtggattt atggatatat tagagaaaat
      961 aactgataga aaaataatgt acacaggaaa aataattgaa gtagaaaaag taacagcaat
     1021 actgccaaat ggcaaggaag tttatcgtga tgtcgtaaga cataacggag catgcggtat
     1081 agtcgctctg gatgaagata tgaatattgt tctagaaaag cagtacagga ttgcttttga
     1141 tgatatactc attgaacttc cagcaggcaa actggataag gatgaagaac cgctgcagtg
     1201 cgctataagg gaattggaag aagaaaccgg ttatgtcgct gacagtatga ctcatatact
     1261 gacagtggca tcctctccag gcttttcaga tgagactgtt catttgtttt tagcggaagg
     1321 ccttacgcag aaggaaatga aatgggatga ggatgaacgg ttaatagtat ggaaagaacc
     1381 tttgataaaa gcaaaacaaa tggtacttga aggtaagata aaaaacggac ttgcagttat
     1441 gggcatattg gctgcatgcc agataaaggg gataaactga tataatgata aaattctgtc
     1501 ctttattcag cggaagcagc gggaatgccc tttatcttaa atacaatgat acatcaatcc
     1561 ttatagatgc aggattatct aaaaaaagaa ttactcaggc tattagtgat ataggagaag
     1621 atgcatcaaa actgaatgca atactaataa cccatgaaca catagaccat acatccggta
     1681 taggcgttat ggaaagagga tataatattc cagtttatgc aaatatctct acatggaaaa
     1741 acatgccaag ttttttagga aagattaggg aagacaataa gaaaactttt gaaacaggga
     1801 agaagtttac cattaatgat atagaaatac tgccgtttga aatacctcat gatgcagcag
     1861 atcctgtggg ttacagtttt tatctggatt ccagaaaagt tacatcagta acagatatcg
     1921 gccatttaac tgatacgata ctagaaaata ttaaaggcag cgacctgcta ttaatggaat
     1981 cgaatcatga tcttgatatg ctgattaaag gtccctatcc atggccttta aaaaacaggg
     2041 taaaaggaaa tttcggtcat ttatgcaaca aagactgcgg tgaccttata gctagatata
     2101 tagaattcgg gttaaagaca attgtacttg ggcatttaag tgctgaaaac aataccccac
     2161 aattagcata tgatacaatt aagcagtgta tcgaaagttt cggatatgaa ataggaagag
     2221 atatagacct tgatgtagca aaaagagaag ggtgtaacag agtatttgag ttatgaagat
     2281 aaatataatc tgtgtgggaa agctaaaaga aaagtatctt gctgaagcgc aggcggaata
     2341 tataaaaagg ctgtcaaggt tttgtgatgt aagtatatta gaactaaatg atgaaaaaga
     2401 tgatatccct gatgcaatag agaaagaagg aacaaaaatc ttatcaaaga tagatactaa
     2461 agattatgta atatgtcttg caatagaagg taagaaacta aacagcgagg ctttttcaga
     2521 aaagattata tcagtatttg attcagcaaa acctgtcatt gattttgtaa taggaggaag
     2581 cttaggctta tcagataatg taaagaaaag agcagatatg cttctttctt tttcagatat
     2641 gacttttcct catcagctga tgagaattat tcttcttgaa caggtttaca gagcattcaa
     2701 gatagcgaat aatgaaaatt atcataagta aaggtataaa ttagagatga aaaacataat
     2761 tactattcag tatacgaagt ctattcacca tacaaatgga atggttggtt cttggactga
     2821 ctgggatttg tctgagttcg ggatttccca ggctgacaag ataggagaaa acctgaaaaa
     2881 agaaatatca gataaaaaat atgtcatgta ttcatctgat ttaatgagag caaagcatac
     2941 tgcaatgatt gttgcagatt atcttggaat tactccaata tatactgaag tattaagaga
     3001 aagaaactta ggacaagcag taggagaatc agttgaatgg ttaagaaaca acatgacaga
     3061 ggagaaaaca attgatgaca gaatgtttta tgatgcagag acaatacacg aacaatatga
     3121 caatctgtta aaatttcatg agcagataat gaaaagtgac aatgagaata tcctaatagt
     3181 atcgcatggc ggtacattaa gtgttttcca tgcaatatgg cttggattgg atgcagacat
     3241 gctgaacaga tgcaatcttt atgggaaagc aggcggagtt tcatttatgg atattgatat
     3301 aagaggctca aaaaggataa ccagattaag cgatatgtca tattcattaa tttaagttat
     3361 agaatggtat cataaaagta ctttgataaa atatataatt agaggtgaat attttgaaac
     3421 gaaaacgctt agatcgagat atatggacat ttgataaata tccatattat caattgaggg
     3481 tagatatgga agattttcat ggtcttgtat gctttataca gttaaagggt ggagttgctc
     3541 atatcaatgg aggacagtat caatattggg ataggcctaa ggctggaaaa gttgcaatct
     3601 gtggtaaggg tatgatatgg atgcaattaa ttcccgatga taagagtcat gttctaactg
     3661 taaagtattt accagataat actgtatcta tatggtatgc agatattgtt gaaaatattg
     3721 aatatgattc tgatggtgta gcagtttttg ttgataaata tctcgatgtt acttttacac
     3781 cacaaggtga tgtgagtatt gatgatcgcg atgaactcga tgcagcattt caatctggag
     3841 agctttcaga gcaacagtat gctgcggcaa tagaagaagg tgacgcagtt gtcaaagagt
     3901 attgttctga tttagtaaaa acagaaaaca tttgctataa aatcctgttt tatgttttag
     3961 aaaaaatcaa aaacggagaa aaacagttta agcatatagt ataagtttga tgagatgctt
     4021 gttctagtat tcagattcaa caacataaag ataatggtgt actattcttc tgtttttagt
     4081 gtatagttaa cagaaggagt ttgtatcatg agattgtttc atgtcagtga agaaagtaat
     4141 atccaggtat cttttctttt acagttaaaa atcaagatat cctaaaaaaa caccgtattt
     4201 atcgtggtta tttaagatgt acagatgtaa atagttgtgc agtaaaatac tattatttaa
     4261 cttttctcaa attattttta atatctttta atggaatata tttcaagctc aagtctatgc
     4321 caagcatctt ccctatatcg caattgagaa tgaattcaat aatgtaatag cagttaagga
     4381 tatgccattt aggtacattg atgtaaatcc gatagataaa gtggtagata atgtggtaga
     4441 taatgtggta gataaaattg aagggttgaa cgaaactcaa tgtaaaatat tgcttgcaat
     4501 tagaaataat tctaatatta cgcaatctca attatcagta gcggtaggta ttggcaagac
     4561 agctattcaa aataatatag tggtacttaa gaaacgtgga tatatagaaa gggtgggctc
     4621 aaataagacg ggttattgga aagtaaacga ataaaaagaa ggacagtttt tcttgttgaa
     4681 tacaccccag ttccagaaaa tgtaccagac tatgaaacgc tgatattatt taagaagaaa
     4741 ttatctgaat tgtatcttat aggaatgggt gttacaaaag aacaaataca agattatctg
     4801 gacctgatta ttatagcaag aaaagcagag tgccctaatg agtatttgta gaagattttt
     4861 aataatgtat gatgaattta aatagacaaa gaagcaatat ttttgctaag tttttctgat
     4921 atatgcaaat aaaactgatt attgcataat aaggataata aatgtattgc gtttgtgctt
     4981 acatatgcat ataataagat acaacactaa ataaaatgga ggagttaatg taatggcaaa
     5041 aacagcaaat ctttatgcaa gaatagaacc agatgtaaaa gaaaaagcag aggaaatcct
     5101 gtctgaaatc ggtgttcctg tttcaagtgc aataactatg ttttataaac agattattct
     5161 tcataaagga cttccttttg aggttaaatt gccgaaacct caaaatatag aatcagtaat
     5221 aaaatataac aaactttata gcgacagaga aaaatctaaa ggaagcgtat tatctgaaaa
     5281 gcaagagaat tatatagaaa agacggatgt tgctaatgaa gttttgaaga aactacttga
     5341 tgaaacgcct gaaaagcaaa aacccgagat catcaactac ataacttatc taaaattaag
     5401 ggatgaaaga aacttatata aggaactatt aactgcaagt gaaagcagtc tggacttctg
     5461 gttcaatgac atagatgatg aggaatggaa taatgtataa acaaggtgaa atactgctaa
     5521 tatcacttcc attttcagat ttgacatcaa gcaaaaaacg tccagttctt gttatctcaa
     5581 atgatgaaaa caataggaca tcagaagata ttgttgtagc ggcgattacc tcgaacatat
     5641 gcaaaaggaa atactcagta atgataagca gcagtaattt aacagaaggc acattaaaac
     5701 gggattcatg cttgagagca gataaaatat tttctttatc acaaaatatt gttattcgga
     5761 agtttggtaa agtagaacaa aatgtcatta atcaggtaat aaactgtatt actgatttaa
     5821 ttacaaataa atagcagatt gtgagatatt aaaatcacta tttgcacttt ttaaacaccc
     5881 agtctctttg aatattaaaa gatatgaaga ataataccgc ttccacccct atttttataa
     5941 gtgtgatcat cagctctttt tcagcaggaa atacattgga cagtacagtt acaatacctg
     6001 ctgatataag cataagggat atgcataata tgtagtagcg aagcaggcat tttctgtaat
     6061 cgcatttatg cttgaaaaca gaattcctat tgaaattgaa attaaaaaag gatgagactg
     6121 ctcttgcaat tactgtactt ataagtattc ttgtgtcttc aagaacactg gcaaatatta
     6181 ataatgatat gaagaatatg atgttgtcta taaagaagga tattattgag ctcatcataa
     6241 atttaaatat aagcttgtag attctaaagg catctcttac cgcattgaaa tgcgacccaa
     6301 cattcttgtc ctcatatact gtggttattt ccacttctct aaatggaatg cattctggtt
     6361 tcatctctaa aagcatatta gtctcatatt caaaacgttc gccttttacg ctaagaagaa
     6421 aagatatatg ttttactggg aaaaccctca aacctgtctg tgtgtcatta atgtttattc
     6481 cgcagcctgt cttaaataca aaggaagtta tcttgttccc taagaagctt tttaagggaa
     6541 catgtgattt atagaagttt ctggcaccta aaattactgt ttcatcatca gtcatctgct
     6601 gtgagcattt taatatgtct ttaggcagat gctgtccgtc cgcatctgcg gtaactacac
     6661 ctagaatatc tttatcctgc tgttgaatat attgaaaggc tgttttgaga gcagctcctt
     6721 ttcctttatt tacatcatgc tttaataatg tgcactgttc taaagaagat acttcactaa
     6781 aaaccttgtc ataatcagaa ctgcttccat cattaactac aataatattt gaaaatcctt
     6841 cttcgattaa tcctgttact gtcgaaacaa gtttctgtgt tggctttaat gatggcagga
     6901 ctacggttat tttaaggccg ttatctttaa atgtcatgat atatctctcc ttgcttattc
     6961 tgataacata tcagctactc gccatttgtt attatacagc ataaatacca gctcaaacga
     7021 atcttccaga gttttagttt cataatatgt tttttgtgtc gcgctgaaac tcatctggca
     7081 ggtgaaacat gtttcactta ttggtctgaa atcagtaaca ctaaggtagt tgtactctaa
     7141 agtcgaaccc attacccagt aaaggcttgg aatcgccgac ttcaatctgg tttgtaaaac
     7201 gctgtcttga atcagataag cgtttagggt tgggaagtat ccccatatat cacctgtggc
     7261 attagtagtg aaattaatat atacttttgc aaaacccaga gcatactgtt cataatctgc
     7321 tttaagtttc tctgactcag agtaatgata aatgatattc ttcttatctg atgcttcgat
     7381 tttttctaac gtaagaccat cttctccata acactcaata ttaggctcac tgtatagatt
     7441 ctcaacgaga tatctgttta gtggaggtat ttgtgtatat gtgactaagt tgtcaaagcc
     7501 ggcataatat tcttcactgt tttcaatata ctcttctgta agcaatattc cgttaatata
     7561 tacttttgct tctttaggag cagttatatt atatgaaagc gtttctttga taactaaggg
     7621 ataaacatat tcattaccag ctctgtttag ttctaagatt atacctttat ggtcctttac
     7681 aagtatatca gcttctaagt aaattcctgg tatctcatat ttttttcctg ttggtctttc
     7741 atcaaagggc tgtagtacat cttcataacc tgaataagca actgaactat cggtaacata
     7801 tgaatcactc acttctatat cattaacata aacatcagcc aattttggca ctgttatact
     7861 taaagtcttc tgctcaggat taagtacgga atcaagaaca gtaaaggtgt ctgcctgcca
     7921 tagattaaat ccgaattttg cagtgttatc acctttactt gtcagtacaa ctttacataa
     7981 atcagcagag tctgtatata tgatatagac aggcagtgtg tctgtatatt ggccgggcat
     8041 cttgcggtat ttatattctt catttttgag atttgaaaaa aacaattgat cataaattgt
     8101 gtttatatct tcaaactctg atgcttcaat cttttcattc tcagtcatca ttgttcttat
     8161 tgtgttatca tcaactgttt taacaaattc attcataaag ttttctggtc tggataattc
     8221 atatgatttc atgtattgcc agaaaagagc taagccgata agaatgacta caataaagca
     8281 tgcggaatat atcgctaggc cttttatgaa tttctttttt tgtttttttg tcttcctgtt
     8341 atatataact gctttttttt ccataaatca ccttactaaa aatgtagtag gcagagctct
     8401 gggccctgtt actgatgcgc agacattcat tacttcacct tcatatatca tcagtgtcga
     8461 ggatccacca tccagatttg aagcattgac tgctccaaaa tccagcatga tatccgctaa
     8521 atccccatat gtagcgccta atgaattcat acgccttccg tcaaccacca gcattaagac
     8581 ggctccatcg atacgttgtc cgattgcagt tctgggattt agaccgctta taaggtctga
     8641 ctcaaaactc tgaacttttc catttattat caaagcaggt ccgaaactta ccgcccattg
     8701 tatgttggta tccattgctt cttctgttgt catcatgccc acatgcaata tgcctgtatc
     8761 atcaaaacct gctacattta gtttaatatt aggttcgccc cagactactt ctccgttaca
     8821 tataaccata ccgtcaggag tgccttcatg tccgcctcca aaaggatcat agaaaccccc
     8881 tgcattaata cctgctattg catcgtattt ttcaaccatc tcatcaagct tcagtccttt
     8941 accgccaagt tcatcaggag tcccgacaaa taatcttaac gggtctttga caataagcat
     9001 gactccttca taaaggcttc ccgatattct gacaacctca atatcttttt cctcttcagt
     9061 tatttcagga atatctgttt cactgtcatt tggctgtgta gaaggagtag gagtaggaat
     9121 ctcaataagt gaagtatcag ttttttcact tggttccttt actatttctg ataccgtaag
     9181 cttggcaagt tcttcatcag acagatatat atttgcaaga aaacctatag cgcttgtttc
     9241 ccttactgat agtacgaata gtttctgggc agtaggggaa ggacccttta ccagaacata
     9301 tattacccct aacaaagcta caactattaa agccaaagta gtgagaaata ctaccagagc
     9361 tttcaatata gctgaagttg ctttttttac gatgcttctt ttgtctgtca ttatgccccc
     9421 ttacaacatg tcttcatata ttatattcac aaaccaatca ttttccaata caatgttgta
     9481 acaagtatta acctgatata tcataagata acgaaaggta tagttatttg atgcataaga
     9541 aaacttttaa gttcccgata ttgattatga tagcagttac gatgtttatg ctaagcggct
     9601 gtaataaacc ggtaattact ccaagtgaga cgaataaacc ttctccttca atatctgcta
     9661 tacctgaaac agaaacacca tctgaaacac cgcaacagac acctgagcag acaccaatac
     9721 agagcgaaga acctgttgaa cctactgaag aaataaaacc tgacgcagaa gatataacca
     9781 agaaggtata tattgatatt gacgggcatt attctgaaaa gctttcagat gacaaccact
     9841 acacaaaata tactctgaac aaaggctcag taataaatat atctgcttca gagcagatac
     9901 actctttgta tatagtatgg gacaggatac caggagaatg gacccttata gctaatgatg
     9961 agaaggtcac aggcggcaag aacggcttta ttcatgaata tatagagctt tctaatagtt
    10021 caaaaagagc aagtatcgaa ttaacaaaca attcagccat aatatgtgat gtctatgttt
    10081 tcacatatgg aaaccttcca aaatgggttc agacatggga tatgccatat gaggatgcgg
    10141 acatgctgtt attatctact catgctgatg atgagcattt atactttgga ggtatgatgc
    10201 catattatgg aggggaactc ggatacaagg ttcaagtagc atatcttgta aaccactgga
    10261 atgaaccata caggcctcat gagcttttaa acggattatg gactgtcgga atgactgcat
    10321 atccgataat cggagaattt gatgatctgt attcaccaag tttggagcat gcgaaaacta
    10381 tatatcctct cgaagatgta ctggattatc aggttgagct tttaagaaga tttaagcctg
    10441 aagtggtaat aggacacgat ttaaaaggcg agtatggtca tggagcccat atgctgaacg
    10501 catacgggct tactttagca gtggaatatg ctgcagatga tactaaatat atatcatcat
    10561 atgaaaaata tggtttgtgg gatactccca aactgtatct tcatttattt gaagataaca
    10621 agattttaat gaactgggat atgccactag aaaaatttaa tggctttaca gcttttgaaa
    10681 tggcggtaaa aggttatgat tgtcataaaa gccagcatat atggtcattt gcagtaagac
    10741 agggagagag ccagtatgac tgcagatggt tcgggttata cagaagcctt gttggtcctg
    10801 atatacaaaa gaatgatgtg tttgagaata ttgtttttga agataaatag cataaattgc
    10861 tagacaattt ttatttatta ttcgcaaata aatcttttat tatcactgta atagaaagga
    10921 tatatggtgc attacatgta cttattttcc ggttctcatt ctttcgagat ttattatcct
    10981 tcatagtaac tgactcatgg atacatcagg ctgactgtgt tcagtctgcc tgatgtaata
    11041 taaatgtgaa atttaaatga acattttatg ctatttattg acataaaaag agtaacatga
    11101 taccatttag taaatctaag caaaacaata tttttagaca aacggaagaa gctgtcatcc
    11161 gcgcatttat ttcattaaat caagaggtga ataatggaaa aagacaaagt aaaaggtaga
    11221 atagcggtac tcataaccgc tggcatcctt atagttgcag gagctgtgct tctgttcata
    11281 ttattaggga acaaggaaga aggtccatca gtatatgaga tacctgtaca gaggataaac
    11341 ggaacagaag gagcaagcct ggatatgcca tatgaataca tgcggagcat gatgaacagt
    11401 aaggagtccc ttcttaataa tgcagaggat atagaggctc agacaatcat agcgaaaagg
    11461 tctgatggta ttaaagatgg tattagagtt ggatatgtat taacagaaaa gtggtataac
    11521 ggtcctgtcg acaggctttt gtatgtgcag ggtataacca gcataaagac aatcgagaga
    11581 aaagataact gcaaagtgac ctttatcgat gggtatgact cgtcaaatct gatgaaaaaa
    11641 aatgatgggg aagtgggggg atatgccatt ttgccggaat ggatgaaaga caacatagct
    11701 cctgaataca aggttgacaa tattagcata ttactaatga aggaagaacc caaaccggtc
    11761 ttaagaaagt tcaaagtaat aggcttctac aaagccgaca ccgaagacac agtatatgct
    11821 tccatgtaca gctatgcaga actgtataag ttcgaggagg atgtcttaga cagtctcatt
    11881 atgtcgctca gcatatatta cgaggatgga gccaatatga catcactgta cagcttcatg
    11941 aacgactatt tctcagattc ccaaacgaag aaagaaggcg agaacttggt aaacatggat
    12001 tatgacttct tctatatacc aattacagaa caatagagaa aacatatgaa cgggaatgga
    12061 ggaatcaaat gagatatgct cttaagagac tatgcaggac gccagtgaag acgataatct
    12121 tcatactgct cataagcctt tctgtaagcg cgttaagcat aagtgctgga atgtggaaat
    12181 actcatatga cagcataaag cattctaaag atgcgttcac tacgataggc atattgagag
    12241 aaattgagtt cactgaacag atatacaaga actcggttaa gcctaaatac gattatgaat
    12301 tattaagcaa ggtcaaacaa gccg
//
LOCUS       DAOSXM010000064        11688 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2111
            NODE_3477_length_11688_cov_10.657354, whole genome shotgun
            sequence.
ACCESSION   DAOSXM010000064 DAOSXM010000000
VERSION     DAOSXM010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864178
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 11688)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11688)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:24:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,261
            CDSs (total)                      :: 2,229
            Genes (coding)                    :: 2,218
            CDSs (with protein)               :: 2,218
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 3 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11688
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3477_length_11688_cov_10.657354"
                     /isolate="AMR_MDS_2111"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1374
                     /locus_tag="PLQ68_08210"
     CDS             <1..1374
                     /locus_tag="PLQ68_08210"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXK72676.1"
                     /translation="HLVGMLQRMGIRAPILLLGGIGITLFLDVTMTLAMIAILPIATI
                     LILVISKIGVKLFRKIQLANDDMVRVVRENATGIRVIKALSKSEHERSRFQSVNENLT
                     KREKTAALVMSTINPTMSFLMNAGFVVVIVIGAFRVNEGNTEIGKIIAFMTYFTIILS
                     AMMSITRIFTMTSKATASADRIKEVLEAQNERDITIHKENDLNIEEEISDSVPHVEFK
                     DVVFKYNNSRFLIDHVSFKIPKKGTLGIIGATGSGKSTLAQLLMRFYDIKSGEILING
                     KNIRYMKLYDLRSKFGVVFQNDTLFKNTIEENLNLGRDIDLMQIKEAANHAQAETFIN
                     DAGGFDAPVSIKGMNFSGGQKQRLLLSRALAGKPEIIVLDDATSALDYKTDSKLRAAL
                     NTNYKDSTKIIITQRISSIMYADQILVIDEGKIIGLGKHEELIKNCEIYQEISASQLG
                     GGSNGSS"
     gene            1436..3142
                     /locus_tag="PLQ68_08215"
     CDS             1436..3142
                     /locus_tag="PLQ68_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016609039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXK72677.1"
                     /translation="MGYIWQYKFLLFLALAMTLAANLLSLAGPFLSGKAISAIENGFG
                     KVDFDTVFFYAGLMLGFFAISAILSYLLTIVMVNISRNITYKMRKDVFDKITKLPVGF
                     FDTNQTGDILSRISYDIDVINTSLSNDVVHILAGITTVIGALIMMLVISPIMTLVFAI
                     TVPLAIVVTKKKTKKTRPLFRKRSAKIGELNGFTEEMISGQKTLKAYLREIHTIQKYD
                     ENNDETVEVTYDAEYHGSTIGPTVNFINNLSLSLISVFGAAMYLLGGITIANISSFVL
                     YSRKFAGPINETANIIAELQSALSAAERVFRLLDEDEEKADEIDAVSLDNPYGDIEIN
                     DVNFSYVENVPIIQNLSLKAPKGTLVAIVGPTGAGKTTLINLLMRFYDADSGSISLDG
                     YKINNITRKDLRLAYSMVLQDTWLFHGTVYENVTYGRQDATMEEVVAACKAANIHNYI
                     ENLPDGYQTMINDDGTNISKGQKQLLTIARAMLMNTSLLILDEATSNVDTRTELNIQQ
                     AMRTLMSDKTCFIIAHRLSTIQNADHILVVNSGQIIEQGTHKELIELKGFYADLFYSQ
                     FS"
     gene            complement(3207..4055)
                     /locus_tag="PLQ68_08220"
     CDS             complement(3207..4055)
                     /locus_tag="PLQ68_08220"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012573543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine kinase"
                     /protein_id="HXK72678.1"
                     /translation="MKRQKRVLAIHDISCFGKCSLTVALPIISAAGVECTVLPTAVLS
                     THTGGFTDFTFRDLTEDIMPIINHWKTLNIGYDAIYTGYLGSFEQIGIVSEIFDTYRT
                     KDSIILVDPVMADNGKLYGAFGPDFPKEMKKLASKADAVVPNITEACFMLDKEYKEGP
                     YTKQYIDDLLLSLAQMGPDKVVLTGVSYNDKELGAATYDKKTNEFGFYARNKIEGYFH
                     GTGDVFGSALLSAMIRGKSLIESATVAVDFTVESIIRTYNVKTDVRFGVNFEEGLQKL
                     AEILKK"
     gene            complement(4117..4452)
                     /locus_tag="PLQ68_08225"
     CDS             complement(4117..4452)
                     /locus_tag="PLQ68_08225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72679.1"
                     /translation="MITIKNLNEMDEWLKKPEEKRRAVFFELVNNRRREYIFLKFAKL
                     KGYKWINNDEIKPDEDNCGNIMSLNSEHTMGFVSGQIYMMGKNHEYFAYADTQIVINE
                     MENYLTQVL"
     regulatory      complement(4520..4605)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(4646..5008)
                     /locus_tag="PLQ68_08230"
     CDS             complement(4646..5008)
                     /locus_tag="PLQ68_08230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014903654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4180 domain-containing protein"
                     /protein_id="HXK72680.1"
                     /translation="MEVSIVIKNGKNIAIVKSSNALICDVQSALDFIANINYETGCNR
                     IVIYKSVLCEEFFNLKTKIAGDILQKFINYQIRAAIVGDFTQYTSKSLKDFIYECNNG
                     KDIFFLSKKEEAIQALSR"
     gene            5125..>5560
                     /locus_tag="PLQ68_08235"
     CDS             5125..>5560
                     /locus_tag="PLQ68_08235"
                     /inference="COORDINATES: protein motif:HMM:NF020120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-rhamnosidase N-terminal
                     domain-containing protein"
                     /protein_id="HXK72681.1"
                     /translation="MIFNKINFISAKRELKDNDNAHDPAYMFRKIFSVEKGFQKATLY
                     TAGLGLCYHYINGLSISDDILTPATSDYIKTIWYNKYDVTSFINEGDNIFASLLGNGF
                     FNESFKTPWNYDTAPWREVPKLIYRLDIYYSDKTVVVESDESC"
     assembly_gap    5561..5660
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5661..7523
                     /locus_tag="PLQ68_08240"
     CDS             <5661..7523
                     /locus_tag="PLQ68_08240"
                     /inference="COORDINATES: protein motif:HMM:NF036578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HXK72682.1"
                     /translation="LDIYYSDKTVVVESDESCKVTDDCYITYNQLRSGEHCDLRRYNH
                     TWTQINYDDSKWEQPVRSSKPDNLVFREQKSQPIRECEIFKTSLIKKTYENTYLFDVG
                     QNISGYIRIETEENCNDHLIIRYAENINEDGTLNLNKMDKFYKYSDFQTDKLICNGKK
                     EIWSPKFTYHGFRYIEITGLSKEPEKNMVKAVFVHQDIERISHFECSDELINRLFKMG
                     INATYSNLFHIPTDCPTREKLGWTNDARASCEQMLINFNMKDFYIKYMQDIYDTMLDN
                     GALPGIIPTSGWGYDWGSGPIASGILFEIPYRYYIYAADKTLLENSFPYFLRHLDYIE
                     SRKNQDGFIAYGLCDWAGPYEHLEKSPVPLQLTDTIMYYAFLSVASSSADILGLSLEN
                     KWIAEKENIRAKIMNKYIDDSGNCVVDEQTAIAMLIFYDLYENIYPLKEQLKRNIEKH
                     DYHHYCGMLGLKHLYEALTKCSLSEYAYKIITANGYPSYIDWITNRDATTLCETFQES
                     NSQNHHMWSDFMAWLMKNVSGIQPNPEIPGFEETFINTYFPSNLSHLSSYMITPKGKI
                     SVSWVKTGNEINYTLFIPKGIKAKIKLQNAVFYDSNSNSEECTDGIFNYRLSAI"
     gene            complement(7520..8695)
                     /locus_tag="PLQ68_08245"
     CDS             complement(7520..8695)
                     /locus_tag="PLQ68_08245"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HXK72683.1"
                     /translation="MNIEFDRDYEKHNEEVKKVWESYEKGKPIRMPCTIYGNNRIILL
                     DDKLNTKGYTFEQYFNDPNIMAEVYCQMDYHMRHSIYADHEMGMPKDGWLVYVDRQNL
                     HESGWLGAQIQYAKNNVPFAEPFLDDNNKNMLFSKPIPHIFDNFEGRAYTMYNKLLDI
                     QKSGYTYKGMPIKRVSFSGIGTDGPMTLACMIRGTENFCTDLIDEPEYAHELLDYLVE
                     AIAYRIKALREYFGYNEVIKDIAFADDSIALLSCDMYQEMIFPHHKKLAELLVENFET
                     SNNTIHLCGDAQRHFKFLMDNLRVFSFDTGYPINFKKMVMDLGERIIRINGGVRVDDL
                     MQKTPSDVSKETKRIMDEVMPYTDRFVMREANNLSPCTPVENIYAMYQTVREYGKYV"
     gene            complement(8697..9863)
                     /locus_tag="PLQ68_08250"
     CDS             complement(8697..9863)
                     /locus_tag="PLQ68_08250"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HXK72684.1"
                     /translation="MDFDRDYEKHNEEIKKVWDSYNAKKPIRMPVILGVNNRVIMLDD
                     KLNTKKYTFEQYFHDPLMMTEVCCECEYQMRHNIYADHEMGMPKEGWTISVDKQNLYE
                     CGWFGAEIVYAKNNVPFTEPFLDDDNKYEFLNKPFPKLFGNFEELSFATYNEMLKLQK
                     KGYEYKGKPIKKVVYGSMGTDGPMTLGCMIRGTENFCADLIEDPVYADELMDYLVEAA
                     AFRIKGLRKYFGLKEVNDGYGFGDDSIALLSCDMYQEMIFSHHKKLFTLLLEDFDTSI
                     NSIHLCGDAQRHFKFIKDNLRVYSFDTGYPINFKKLATELGSDIIRLNGGVRVDDLLQ
                     KKPEEVAKETKRIMDEVMPYTDRFVMREANNLSPCTPFENIYSMYQTVREYGVY"
     gene            complement(9898..10263)
                     /locus_tag="PLQ68_08255"
     CDS             complement(9898..10263)
                     /locus_tag="PLQ68_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005949272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXK72685.1"
                     /translation="MKHTFAHFNFNVKDLDKSIAFYKEALGLEETRRKETDDFILVFM
                     KDCETGFMLELTYLKDFDKPAYDLGDNEFHLAMVTDDFDASYKKHSEMGCICYENKKM
                     GLYFINDPDEYWIEILPKR"
     gene            complement(10260..10985)
                     /locus_tag="PLQ68_08260"
     CDS             complement(10260..10985)
                     /locus_tag="PLQ68_08260"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXK72686.1"
                     /translation="MIITHLTHSTFLVESDKAYILFDYFGKGFLPKDIKKNIYIFSSH
                     SHQDHFDMKIFELFGDNPKAFFILSYDIKRKFGKRIKNEYPICQNKITYMMPDKHIEI
                     NDMYIETIGSTDAGVGYIIEYDNKTFFHSGDLHLWTWKEESADYNEKMKKRYFREMKK
                     IHNKKINYAFYPVDLRQAEDYDIGIRYFLDNTETEKVFPMHYFGDYLATDMIFKNDSL
                     SNYYGRIIKIEHENQSFNFEEGK"
     gene            complement(10999..11526)
                     /locus_tag="PLQ68_08265"
     CDS             complement(10999..11526)
                     /locus_tag="PLQ68_08265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HXK72687.1"
                     /translation="MKMTTRKLVITALGLALALVLPFLTGQIPRIGNMMLPMHIPVLL
                     VGFICGWPYGLLLGFVAAPLRSLIFTMPPMFPVALAMAFEMAAYGVLCGILYRLLPKK
                     TINIYISLIGAMIGGRIVWAIASYILFAIAGNSFTWEIFAAQAFVNAVPGIILQIVLI
                     PALVIALKKANIIKD"
BASE COUNT         3825 a         2070 c         1808 g         3885 t
ORIGIN      
        1 catctggttg gaatgctcca aagaatgggt attagagcac ctattcttct tttaggagga
       61 atagggataa ctctgtttct agatgtaacc atgaccttag caatgattgc catacttcca
      121 atagcgacaa tacttatttt agtaatatcc aagatcggag ttaagttatt tagaaagata
      181 cagcttgcaa atgatgatat ggttagagtt gtaagagaaa atgcaacggg tataagagta
      241 attaaggctt tatcaaagtc tgaacatgaa agaagcagat ttcagtctgt aaacgaaaat
      301 ttaacaaagc gtgaaaaaac cgcagctcta gttatgtcca ctattaatcc tacaatgagt
      361 tttcttatga atgcaggatt tgtagtagta attgtaatag gtgcattcag agtaaatgaa
      421 ggaaacacag agattggtaa gattattgcc tttatgacct attttacaat catcttaagc
      481 gcaatgatgt caattacaag aatattcact atgacttcaa aagctactgc ttcagctgac
      541 cgtataaagg aagtacttga agctcagaat gaaagggata taactattca taaggaaaat
      601 gatttaaata tagaagagga aatatctgat tcagtacctc atgttgaatt caaagatgta
      661 gtatttaaat ataacaacag cagattttta attgatcatg tttcatttaa aattcctaaa
      721 aaaggaacac ttggtattat tggtgctaca ggatcaggaa aatccacctt agctcagtta
      781 cttatgaggt tttatgatat aaaaagcggc gaaatcctta ttaatggaaa aaatatccgt
      841 tatatgaagc tttatgattt aagaagtaaa ttcggagtag ttttccaaaa tgatacactt
      901 tttaaaaaca caattgaaga gaatctgaat cttggcaggg atattgatct aatgcagatt
      961 aaagaagctg ccaatcatgc gcaggctgaa acattcatta atgatgctgg aggatttgat
     1021 gcacctgttt caattaaagg catgaatttt agcggaggac agaaacaacg tcttcttctt
     1081 tcaagggctc ttgcaggcaa acctgaaata atagtactcg atgatgcaac cagtgcttta
     1141 gattacaaaa cagattctaa attaagagca gctttgaata ctaattataa agattcaaca
     1201 aaaattataa tcactcaaag aatcagttct atcatgtatg cagatcagat attagtaata
     1261 gatgaaggaa aaataatagg acttggcaag catgaagaat taataaagaa ttgtgagata
     1321 tatcaggaaa tttccgcatc tcagttagga ggtggtagta atggctcctc ttaatccaaa
     1381 tccaaccgaa aaaaaattat ccaaaactga aaagaaacag gtatttttca gacttatggg
     1441 ttatatatgg cagtataaat tcctactgtt tttagcttta gctatgacat tagcggcaaa
     1501 tctattatct ttagcaggac catttctttc aggtaaagct atctctgcaa ttgaaaatgg
     1561 ttttggtaaa gttgattttg atactgtatt cttttatgca ggattgatgt taggtttctt
     1621 cgccatatca gctattctgt cataccttct tacaatagta atggtcaata taagcaggaa
     1681 tattacatat aagatgagaa aagatgtatt tgacaaaatt acaaaactgc cagtcggctt
     1741 ttttgacact aatcagactg gggatatttt atccagaata tcttatgata ttgatgttat
     1801 taatacttct ttatcaaatg atgtagtaca catattagca ggaataacta cagttatagg
     1861 cgctttaatt atgatgcttg taatatcccc tataatgact ttagtatttg caattacagt
     1921 ccctttagct attgttgtaa ctaaaaagaa aacaaaaaag acacgtcctt tgtttagaaa
     1981 aagaagtgct aaaattggtg aattaaatgg ctttactgaa gaaatgataa gtggccagaa
     2041 aaccctaaaa gcttacttaa gagaaataca tactatacaa aaatatgatg agaataatga
     2101 cgagaccgta gaagtaacat acgatgctga atatcatgga agcacaatag gccctactgt
     2161 caatttcata aacaatcttt ccctttcctt gataagcgtt tttggagctg ctatgtatct
     2221 tcttggagga attacgatag ccaacatatc atcatttgtc ttatactcaa ggaaattcgc
     2281 aggtcctata aatgagactg caaatattat tgctgaacta caatctgctt tatctgctgc
     2341 agaaagagtt ttcaggctgc ttgatgaaga tgaagaaaaa gcagatgaaa ttgatgcagt
     2401 ttctctagat aatccatatg gtgatataga aattaatgat gtaaatttct catatgttga
     2461 gaatgtacca attatacaaa atctcagctt aaaagcacct aaaggaaccc ttgtagcaat
     2521 tgtaggtccc acaggtgcag gtaaaaccac attaatcaat ctgcttatga gattttatga
     2581 tgcagacagc ggttcaatat cattggacgg atataagatt aataatataa ccagaaaaga
     2641 tcttcgcctt gcatattcaa tggtgttgca ggatacatgg ttattccatg gaacagtata
     2701 tgaaaatgta acatatggca gacaggacgc tacaatggag gaggttgttg cagcctgtaa
     2761 agcggcaaat attcacaatt acatagaaaa tctgcctgat ggttatcaga ccatgattaa
     2821 cgatgatgga actaatatat caaaaggtca gaaacagctg cttacaatag ccagagctat
     2881 gcttatgaac acaagtcttt taatactaga tgaagctact tcaaatgttg atactagaac
     2941 agaacttaac attcagcaag caatgcgtac ccttatgtcc gacaaaacat gtttcataat
     3001 cgcacacaga ttgtccacca tccaaaatgc agatcatatt ttggttgtta atagtggaca
     3061 gattatagaa cagggtaccc ataaagaact aatagaactt aaaggttttt acgcagattt
     3121 attctattct cagttttcat aacacatttt taaaaactaa agagcaagca tataactgct
     3181 tgctcttaat gtatctaact tttatattat tttttcaata tctctgcaag tttctgaagt
     3241 ccttcttcga aattaacgcc gaatctaaca tcagtcttca cattatatgt acgtattatg
     3301 ctttcaactg taaagtctac tgctacagtt gctgattcaa taagactctt gcctcttatc
     3361 atagctgata ataaagcact tccaaagaca tcacctgtac catggaaata accttctatc
     3421 ttatttcttg catagaatcc aaactcatta gtctttttat catatgttgc agctccaagt
     3481 tctttgtcat tatagctgac accggtaaga actactttgt caggtcccat ctgagctaaa
     3541 cttaaaagaa gatcatctat atactgtttg gtatatggtc cttccttata ctctttatca
     3601 agcataaaac aggcttctgt aatattaggc actacagcat ctgcttttga agcaagtttc
     3661 ttcatttcct taggaaagtc tggtccgaat gctccatata gtttgccgtt atctgccatt
     3721 acaggatcaa caagtataat tgaatcctta gtcctgtatg tatcaaaaat ttcagagact
     3781 atgccaatct gttcaaaaga tccaagatac cctgtatata ttgcgtcata tcctatatta
     3841 agtgttttcc agtgattaat tattggcata atatcttcag ttaggtctct gaatgtaaag
     3901 tctgtaaatc caccagtatg tgtagataat actgctgtcg gaaggacggt acattctact
     3961 cctgctgctg aaataatcgg taatgctact gttaaggaac atttcccaaa acaagatatg
     4021 tcatgtattg ctaatactct tttctgtctt ttcatgttac cacctctttc aatcaataca
     4081 gaataacact attctgctat cttctaatat atacattcat aatacctgtg tcaaatagtt
     4141 ttccatctca ttaataacta tctgcgtatc agcataagcg aaatattcgt ggttttttcc
     4201 catcatataa atctgtccgg atacaaaacc catagtatgc tcactgttaa ggctcataat
     4261 gtttccacaa ttatcttcgt ctggctttat ttcatcgttg tttatccact tgtatccttt
     4321 taatttagca aatttcagaa agatatattc ccttcttcta ttatttacta actcaaaaaa
     4381 taccgctctt cttttctctt ctggtttctt taaccattca tccatttcat tcaaattctt
     4441 gattgttatc attttatgcc tccttttttt atgcatataa agagaggcaa aaaaataccc
     4501 atcacgacag gatgagtttc ccctatcatt caagcgttag caccttgtca ctgacaggtt
     4561 gctgtgtggt cttggagctt gtctctcgca cactctttat aggctatcta ttatattacc
     4621 atatattcct tattttgtca atgtgctatc ggcttaatgc ttgtattgct tcttcttttt
     4681 ttgatagaaa aaaaatatct tttccattat tgcattcata tataaagtct ttcaggctct
     4741 tagaggtata ctgggtaaag tcacccacaa ttgctgctct tatttgataa tttatgaact
     4801 tttgaagtat atctccagct atcttggttt taaggttaaa aaattcttca cacaacactg
     4861 acttgtaaat tacaatacga ttacatccgg tttcataatt tatattcgct atgaaatcca
     4921 gagctgactg tacatcacat atgagcgcat tactgctttt aacaattgct atattcttac
     4981 catttttaat tactattgat acttccatat tgaattacat cttctttctt ataaataata
     5041 tttgttatta actaaaactt tatgaatgaa tctgatactg ttatgcatga tatcatatag
     5101 gtatatctgt aataaaggtg aattatgata tttaataaga taaacttcat ttcagcaaaa
     5161 agagagttaa aagataacga caatgctcat gaccctgcgt acatgttcag gaaaatattt
     5221 tctgttgaaa agggctttca aaaagcaacc ctttacacag ctggacttgg tctttgctat
     5281 cattatatca acggattgtc gataagtgat gacatattaa caccagcaac ttctgattac
     5341 ataaagacca tttggtataa caaatatgat gtaacttcat ttataaatga aggggataat
     5401 atttttgctt cattattagg taatggtttc tttaatgaga gttttaagac tccctggaac
     5461 tatgataccg cgccatggag ggaagttcca aaacttattt accgactgga tatatattat
     5521 tcggataaaa cagttgttgt tgaatcagat gaatcatgca nnnnnnnnnn nnnnnnnnnn
     5581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5641 nnnnnnnnnn nnnnnnnnnn ctggatatat attattcgga taaaacagtt gttgttgaat
     5701 cagatgaatc atgcaaagtg acagacgact gctatataac atataaccag ctcagaagtg
     5761 gtgaacactg tgatttgaga agatataatc atacatggac acagataaat tatgatgata
     5821 gtaaatggga acaaccggtt aggtcgtcca aacctgataa tttagtattc agagaacaaa
     5881 aaagccagcc tataagagaa tgtgaaattt ttaaaacttc tcttattaag aaaacttacg
     5941 aaaacacata tttgtttgat gtcggacaga atatttcggg gtatataaga attgaaactg
     6001 aagaaaattg taatgatcat ttgataataa gatatgctga aaacataaac gaagatggca
     6061 ccttaaatct gaataagatg gataaattct ataaatatag tgattttcag acagataagc
     6121 ttatttgtaa tggcaaaaag gagatttggt caccaaaatt cacataccac ggatttagat
     6181 acattgaaat tactggctta tctaaagaac ctgagaaaaa tatggttaaa gcagttttcg
     6241 tccatcagga tattgagaga atatctcatt ttgaatgttc tgatgaatta ataaacagac
     6301 tgttcaaaat gggcattaac gctacttact ccaatctttt ccatatcccc acagactgcc
     6361 ctacaagaga gaagttagga tggactaatg acgcaagagc ctcctgtgaa caaatgctta
     6421 taaacttcaa tatgaaagat ttttatatta aatatatgca ggatatatat gataccatgc
     6481 ttgataatgg agcgctgcca ggaataatac ccacatcagg gtgggggtat gattgggggt
     6541 ccggtcctat tgcttctggt atactttttg aaataccata cagatactat atttatgcag
     6601 cggataaaac attacttgaa aattcctttc catattttct aagacaccta gactatattg
     6661 aaagcagaaa aaatcaagat ggctttattg catatgggtt atgtgactgg gctggtcctt
     6721 atgagcatct tgaaaaatcc cctgttccat tgcagttaac agataccatc atgtattatg
     6781 cttttttatc tgttgcttct tcttcagctg atattctcgg tctttccttg gagaacaaat
     6841 ggattgcgga aaaagaaaat ataagagcaa aaataatgaa taaatacata gatgattcag
     6901 gaaattgtgt agttgacgaa cagactgcaa ttgctatgtt aatattttat gatctgtatg
     6961 aaaatatata ccctttaaaa gaacaattaa aaagaaacat agaaaaacat gattatcatc
     7021 attactgcgg tatgcttggg ttaaaacatc tttacgaagc attaacgaaa tgttcattaa
     7081 gtgaatatgc atataagata ataacagcaa atggttatcc aagctatata gactggataa
     7141 caaatagaga cgcaaccact ttatgcgaaa catttcaaga atcaaattca caaaatcatc
     7201 atatgtggtc tgatttcatg gcttggctta tgaaaaatgt ttcaggaata caacccaacc
     7261 cagaaatccc aggatttgaa gaaaccttta taaacacata ttttccttca aaccttagtc
     7321 atctaagttc atatatgata actcctaaag gcaagatttc agtatcctgg gtaaaaactg
     7381 gtaacgagat aaattataca ctcttcatac ctaagggaat aaaagctaag ataaaattac
     7441 aaaatgcagt attttatgat agtaattcta attctgaaga atgtactgat ggaatattta
     7501 attacagatt gtctgctatc tagacatact taccatattc acgaactgtc tgatacattg
     7561 catatatatt ttcaaccgga gtacaaggtg aaagattgtt tgcttctctc ataacaaatc
     7621 tgtccgtata tggcataacc tcatccatta tcctttttgt ttctttagat acatcagacg
     7681 gcgttttctg cataaggtcg tcaactctca cacctccgtt gattcgtatg attctttccc
     7741 ctaaatccat taccatcttt ttgaaattga tagggtaacc tgtatcaaat gaaaaaactc
     7801 ttaaattatc cattaagaat ttaaagtgtc tttgggcatc accacataag tgaatagtat
     7861 tattacttgt ttcaaaattt tctacaagca attcagcaag ctttttatga tgaggaaaaa
     7921 tcatttcctg atacatatca catgataaca gtgctattga atcatcagca aatgcgatat
     7981 cttttattac ttcattgtat ccaaaatatt ctctcaaggc tttaatccta tatgctatcg
     8041 cttcaacaag ataatccaat aattcatgtg catattcagg ttcatcaata aggtcagtac
     8101 aaaagttttc tgtacccctt atcatgcaag caagcgtcat aggtccgtct gtccctatcc
     8161 cgctaaaact cactctttta ataggcatac ctttatatgt atatccgctt ttctggatat
     8221 caagtaactt attgtacatt gtgtacgctc taccttcaaa attgtcaaaa atatggggaa
     8281 taggcttact aaatagcata tttttattat tatcatcaag aaatggctct gcaaaaggaa
     8341 cgttgttctt tgcatactgt atctgtgcac caagccatcc gctctcatga agattctgcc
     8401 tgtctacata aacaagccac ccgtctttgg gcattcccat ctcatgatct gcataaatag
     8461 agtgcctcat atgataatcc atctgacaat aaacttccgc cattatattc gggtcattaa
     8521 aatactgttc aaaggtataa ccttttgtat taagtttatc atctagaagt ataatcctgt
     8581 tatttccata aatagtacag ggcattctaa tgggttttcc tttttcataa gactcccata
     8641 cctttttaac ttcctcattg tgtttttcat aatccctgtc aaattctata ttcatactaa
     8701 tacactccat attccctaac tgtctgatac attgaatata tattttcaaa aggagtacac
     8761 ggagacaggt tattggcttc tctcattaca aatctgtctg tataaggcat aacctcatcc
     8821 attatcctct ttgtttcctt tgctacttct tctggttttt tctgtaaaag gtcatctacc
     8881 ctaactccgc catttaaccg tattatatcg cttcctagtt ctgttgcaag tttcttgaaa
     8941 ttaatcgggt atcctgtatc aaatgaatat acccttaagt tgtcttttat gaacttgaaa
     9001 tgtctttgag catctccgca cagatgaatg gaatttatgc tggtatcgaa atcctccagt
     9061 aaaagagtaa aaagcttctt atgatgagaa aaaatcattt cttgatacat atcacaggaa
     9121 agaagtgcaa ttgaatcgtc accgaagcca tatccgtcat ttacttcctt taacccaaag
     9181 tacttccgta atccttttat acggaatgct gcagcttcaa caagataatc cataagctca
     9241 tctgcatata caggatcttc aatcaaatcc gcacagaaat tttcagtacc tcttatcata
     9301 caccctaaag tcataggtcc gtctgtaccc atggaaccat aaacaacctt ctttattggt
     9361 ttccctttat actcatatcc ttttttttga agctttaaca tctcattata tgtagcaaat
     9421 gacagctctt caaaatttcc aaacaacttc gggaaaggtt tgttaagaaa ttcatatttg
     9481 ttgtcatcat caaggaacgg ttcagtaaaa ggaacattgt tttttgcata aacgatttca
     9541 gctccgaacc aaccacattc ataaagattc tgcttgtcta ccgatatagt ccacccttct
     9601 tttggcatac ccatttcgtg atcagcataa atattgtggc gcatctgata ttcacattca
     9661 caacatacct ctgtcatcat cagtgggtca tgaaagtact gctcgaatgt atatttttta
     9721 gtgttaagct tgtcatcaag cattattacc ctgttattta caccaaggat tacaggcatc
     9781 cttataggct tttttgcatt atatgaatcc catacttttt ttatttcttc attatgcttt
     9841 tcataatccc tgtcgaaatc catatgattg ctccttcata ataaatctgc atttattcta
     9901 tctttttggt aaaatttcta tccaatattc atcagggtca tttataaagt ataatcccat
     9961 ttttttattc tcataacata tacaacccat ttcgctatgc tttttatatg atgcatcaaa
    10021 atcatctgta accatggcaa gatgaaattc attgtctcca agatcatatg ctggtttatc
    10081 aaagtctttt aagtatgtta attccagcat gaaacctgtt tcacagtctt tcataaatac
    10141 cagtataaaa tcatctgttt ctttgcgtct ggtttcttcc aaccccagag cttctttata
    10201 gaaagctatt gatttgtcta aatctttaac attgaaatta aaatgtgcaa aagtgtgttt
    10261 catttcccct cctcaaaatt gaatgattga ttttcatgtt cgatttttat aatccttccg
    10321 taataattac tcagtgaatc atttttaaaa atcatatcag ttgctaagta atctccaaaa
    10381 taatgcatcg gaaatacctt ttctgtttct gtattatcta aaaaatatct tatacctata
    10441 tcatagtcct ctgcttgtct taaatctaca ggataaaatg cgtaattgat ttttttatta
    10501 tgtattttct tcatttcacg aaaatacctt ttcttcatct tttcgttgta atctgcactt
    10561 tcttccttcc atgtccaaag atgaagatca cctgaatgaa aaaatgtttt gttatcatac
    10621 tcaataatat atcccacacc agcatctgtt gaaccgatag tctcaatata catatcgttt
    10681 atttcaatat gcttatcagg catcatatat gttattttat tctggcatat tggatattca
    10741 ttttttatcc ttttcccaaa tttcctcttt atatcatatg aaagtataaa aaaagctttt
    10801 ggattatcgc caaaaagctc gaatattttc atatcaaaat ggtcttgatg gctgtgacta
    10861 gaaaagatgt atatattctt ctttatatcc tttggaagaa atccttttcc gaaatagtca
    10921 aaaagaatat atgctttatc cgattctacc aaaaatgtgc tgtgtgtcaa atgcgtaatt
    10981 atcatttgcg ccttcttttc agtctttaat tatatttgct ttcttaagag ctattactaa
    11041 agcaggtatt agtactatct gtaatattat accgggaaca gcatttacaa aagcttgcgc
    11101 tgcaaaaatc tcccatgtga aactgtttcc cgctatagca aaaaggatat agctagctat
    11161 tgcccatact atacgtccac caatcatggc tcctataagg gatatatata tattaatggt
    11221 cttcttaggc agaagtctgt aaagaatacc acatagcact ccataagctg ccatttcaaa
    11281 tgccatcgct agtgcaacag gaaacattgg aggcattgta aatatgagac ttcttaatgg
    11341 tgcggcgaca aaacccagca acaatccata tggccatcca catatgaagc ctaccaagag
    11401 aacaggtata tgcataggaa gcatcatatt acctatcctt ggtatctgcc ctgtaagaaa
    11461 aggtaaaact aaagctaatg caagtcctaa tgctgttatt acaagctttc tcgttgtcat
    11521 cttcataaca tatcctcctt ttctatgatg ttaccacgag acatatcatt tatcaattgt
    11581 taatggaaat tatagtagtt taatatattg ttaaaaattt aacaatctta gcctttttat
    11641 ttgacatatc atttcatatg tagttcaata tgtaaaacga agtaggag
//
LOCUS       DAOSXM010000065        11470 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2111
            NODE_3574_length_11470_cov_11.558914, whole genome shotgun
            sequence.
ACCESSION   DAOSXM010000065 DAOSXM010000000
VERSION     DAOSXM010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864178
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 11470)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11470)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:24:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,261
            CDSs (total)                      :: 2,229
            Genes (coding)                    :: 2,218
            CDSs (with protein)               :: 2,218
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 3 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11470
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3574_length_11470_cov_11.558914"
                     /isolate="AMR_MDS_2111"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            138..1268
                     /locus_tag="PLQ68_08270"
     CDS             138..1268
                     /locus_tag="PLQ68_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009221645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HXK72688.1"
                     /translation="MADIFSNVLEIRWEMVLMWVIGALLIWLAISKDMEPTLLLPMGF
                     GAILANLPTSGAITQVINGNTEEGVLDVLFNAGIANELFPLLLFIGIGAMIDFGPLLS
                     NPKMLLFGAAAQFGIFFTFALAVLLGFDLKDAASIGIIGAADGPTSIFVANYFNSNYI
                     GAIIVAAYSYMALVPIIQPPVIRLFTTKKERLIKMPYEQKNVSTTVRILFPIITTAIV
                     GILSPRSVALVGFLMFGNLLRECKVLRLLSESAQKELANLVTILLGITIASKMRYDLF
                     VRVDTLIIILLGLVAFTFDTIGGVLFAKLINLFAKKKINPMVGAAGISAFPMSSRVVH
                     KMGLKEDPTNFLLMHAAGANVAGQIASVVAGGVMISLVEKLI"
     gene            1281..1430
                     /locus_tag="PLQ68_08275"
     CDS             1281..1430
                     /locus_tag="PLQ68_08275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="OadG family protein"
                     /protein_id="HXK72689.1"
                     /translation="MFNRFDLDNFLQTPRVVLLGMLGVFVALLVIYVSILLLNRVFRD
                     KKKKD"
     gene            complement(1759..1920)
                     /locus_tag="PLQ68_08280"
     CDS             complement(1759..1920)
                     /locus_tag="PLQ68_08280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72690.1"
                     /translation="MNILICDVVTPVILYPIIYWPFVLAGLVLIVIGILLFRFLKRKN
                     MQNKTEEKK"
     gene            complement(1917..2915)
                     /locus_tag="PLQ68_08285"
     CDS             complement(1917..2915)
                     /locus_tag="PLQ68_08285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72691.1"
                     /translation="MTLIAYSIAHFLTDAVCASAIFSSQEMIPYILMYDLLAFSTQGI
                     TGIMADVIKRYRHIAIAGGLMTALAALLLMPVPLKVCLIGIGNSAFHVGGGAAVLKGS
                     SNKAAPLGIFVAPGSMGLLLGMLFPSIMIYVSIALFLISIGLIWMKEYQSIEPSKDSS
                     TFSMSGISKKVYTLIIIAIILISVSVRSMASYSISYPWKDTITLSILLGVFVMMGKAA
                     GGFILDKIKAVPVIILSILISGPVIAFLSSSIVPSLVGQFLINCSMPLTLYMLYRMLP
                     DYPGFAFGLAASFLFPGMAVGLGLQLSNFIIMLIFMSNAILLYIAVKIMKKGSINV"
     gene            complement(2912..3847)
                     /locus_tag="PLQ68_08290"
     CDS             complement(2912..3847)
                     /locus_tag="PLQ68_08290"
                     /inference="COORDINATES: protein motif:HMM:NF016424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HXK72692.1"
                     /translation="MKKILIIITLILVLTCNVVFAADIPEPTSDFYVNDTANVLSEQT
                     KKLIIDKNAALYDKTGAQIVVVTVEFTNTASIADYAYELFNEYGIGSKDNNNGLLLLL
                     SIGDDDYYALQGAGLERTFTSGTISYLLYEYLEPYFAQQEYDAGVLSVFNAFYEEMLA
                     VHDTAIDTPVEYPNEYEYYPEEYYYGPDTSINIFDVFRTFFIIIVIIAVLISIFGTRK
                     STKRTYSSTQTRSSSSNSNIWPFLGAMYLLNKNKNNSSFSNLNNNHNTINRSSSSFRG
                     TSSFGGGSSFGGRSGGFHSGGGGVSRGGGAGRGGR"
     gene            complement(3882..4409)
                     /locus_tag="PLQ68_08295"
     CDS             complement(3882..4409)
                     /locus_tag="PLQ68_08295"
                     /inference="COORDINATES: protein motif:HMM:NF015944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HXK72693.1"
                     /translation="MKFKENRTLAWILVIVIMITSVLISGYVSLSSQKKKVLNSFYDT
                     MDNDLNTKSSYADNLAGIASRYLDSNSKYITDMSKARNMLLEAENPQEKYVASVNITN
                     AASAIYDVLGTMELTSTDDRLRRSNYADILAVDDILRRSDFNKNVDEFNEELDKFPAS
                     IISALTNIEKADYFR"
     gene            complement(4409..5443)
                     /locus_tag="PLQ68_08300"
     CDS             complement(4409..5443)
                     /locus_tag="PLQ68_08300"
                     /inference="COORDINATES: protein motif:HMM:NF017617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HXK72694.1"
                     /translation="MSEITLTEKDRELALEYGQKLDIKSSASILQYGADAQRKITSFS
                     DATLEKVRSRELGEIGDMIGSLMAQLKDFTADQDSGGFFERIFKKTKRYIDVLKARYA
                     KAEANVSKIVAVLEDHQVVLLKDITMLDKMYEMNLDYIKDLALYIEGGKAKLNELRET
                     KLVELRDKAKQSQLPEDAQAANDFANMLDRVEKKIYDLEITRNIAVQMAPQIRLIQNN
                     NNLLTEKIQTTLINTIPLWKNQMVLTLSITHSQNAMKAQRAVTDMTNELLLKNAESLK
                     QGSIDIAKESERGIVDIETLRTTNESLINTLDEILRIQEEGKTKRKEAEAELNKIEEQ
                     LKNKLLEFKN"
     gene            complement(5461..6078)
                     /locus_tag="PLQ68_08305"
     CDS             complement(5461..6078)
                     /locus_tag="PLQ68_08305"
                     /inference="COORDINATES: protein motif:HMM:NF021617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HXK72695.1"
                     /translation="MEIRHKSAMPIYLAGAVWVLYCLIFSMHKLFHIIIALSLFIAAY
                     LILRKKFPDRVEIVEETIKYTGAKEIDQAIEEGNKYLEDMRKANELIESPLISNKIDS
                     LSEITKKIFNYVSEKPEKLKDIRRFLTYFLPTTVKLLNSYARAEAQDNKGENINQIKN
                     DIEGAMDGIIESFHKHLDNLYADEAMDIDAEITVFDSMLKAENLK"
     gene            6276..8525
                     /locus_tag="PLQ68_08310"
     CDS             6276..8525
                     /locus_tag="PLQ68_08310"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013150831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HXK72696.1"
                     /translation="MLTENSIKVLERRYLKRDSQGNITETCDDMFRRVAKTIASADSE
                     FLGKEDIAKTEKDFYDMMVNLEFLPNSPTLMNAGRTLGQLAACFVLPIEDSMEGIFET
                     VKNAALIHKSGGGTGFSFSRIRQNGAAVSSTGGIASGPISFMKVFNTATEAVKQGGTR
                     RGANMGILRIDHPDIKAFITCKENSTELNNFNISVGLTEQFMEAVEHDSTYELIDPST
                     KQVVGVESAKEIFDMIVQHAWLNGEPGIVFLDRINKVNTVPGLGEIESTNPCGEQPLL
                     PYESCNLGSINLYTMLKEDKSGFDYEKLGRCVVKAVHFLDNVIDVNAYPNEKIEKNSK
                     ATRKIGLGIMGWADSLYAMSIPYNSEEALDLGYELMKFINEESKKASMSLAEIKGVFP
                     AYDKSIYPAQDIKVRNATTTTIAPTGTLSIIAGVSSGIEPVFAISYIRNVMDNTEMAE
                     VNPVFEQIAKERGFYSEELMKVIAKKGSLAHIDEIPEDVKKVFVTSHDIDPIWHVKMQ
                     AVFQEHVDNAVSKTVNLRNEATLDDVREVFMQAYAMACKGVTLYRDGSRASQVLSVSK
                     QPPKEEHTAYVTPKPRPRITTGLTEKFKTGCGNLYITVNYDENGEICEIFTNTGRAGG
                     CPSQSEATARLVSLALRSGLDTKEIIDQLKGIRCVSTIRHKDLGILSCPDAIGRMIEK
                     VAKMNGELKEEVKRTIPYIQYGKEKDTPVVKKEQKTTDSRPCPECGNPLESDGGCVIC
                     RSCGYSKCN"
     gene            8638..8973
                     /locus_tag="PLQ68_08315"
     CDS             8638..8973
                     /locus_tag="PLQ68_08315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72697.1"
                     /translation="MANKKEHYVLAVRDLDKALADIASGKVKMPVDNSKYAEIFSNIV
                     RRCSNLEELNKFIRQNKMKKNECIHWWEGILDDGYELITVQYNAPDENFVELAGSENL
                     IKYVTSVKG"
     assembly_gap    9179..9278
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9279..9565)
                     /locus_tag="PLQ68_08320"
     CDS             complement(<9279..9565)
                     /locus_tag="PLQ68_08320"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family glutamic-type intramembrane
                     protease"
                     /protein_id="HXK72698.1"
                     /translation="MTDIIRIIFSALSPVAALIILLLVVDRHDKEPAKLLIKQFIAGC
                     LISIPAILIEGFLERFNIFPETLIGIAYTSFIVAGLTEESLKRASVYVVSY"
     gene            complement(9575..10141)
                     /locus_tag="PLQ68_08325"
     CDS             complement(9575..10141)
                     /locus_tag="PLQ68_08325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HXK72699.1"
                     /translation="MIYLELFWEFFKTGLFSIGGGLATIPFLYDIGERYDWLDTSIIP
                     DMIAISESTPGPIGINMATYTGYMAAGMLGSLIASLSVILPSVIIVILLSKLLVSYSQ
                     DRRVLNAMYGLRPASLGLITAAAFALLKVSVFTFETFVETRNILDIIDIKALILFVCL
                     LVARIFYRRIHPIIFIVFAGIVGIIFKF"
     gene            complement(10138..10692)
                     /locus_tag="PLQ68_08330"
     CDS             complement(10138..10692)
                     /locus_tag="PLQ68_08330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004816207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HXK72700.1"
                     /translation="MRALFELYWIFLKVGLFTFGGGYTMLPILQKEIVEKRKWLATEQ
                     LIDFYSLSQCSPGVIAVNVSTYIGYNRKKYLGGIFCALGVITPSIIIITIIATVLTNF
                     LHIEAVNHAFAGIRAAVAALVIVTIIDMYKKSIVDWVCIILYIGSFVISVFTSISPVF
                     IVLVSAVVGLLLGTRMSERKKRDK"
     gene            complement(10886..11341)
                     /locus_tag="PLQ68_08335"
     CDS             complement(10886..11341)
                     /locus_tag="PLQ68_08335"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4143 domain-containing protein"
                     /protein_id="HXK72701.1"
                     /translation="MVYLLKPYSNNSIKRVFKTPKLYFLDSSLVAYLTRWLTPDTLVE
                     GTMNGHFFETFVVAELLKSYTNAGKEENFYFLYDSNGREIDLLIFQDNTLFPIEIKKH
                     TCPTLKDIKNFEILDSIKGINISEGGVICLSQELLPLTDKHRIIPLWAI"
BASE COUNT         3798 a         1948 c         2058 g         3566 t
ORIGIN      
        1 ctttctatgt taaagtatat ataaataaat atgttattgc aactaatcgc ttgacgagac
       61 ggattgatta aaagtatgat atatatcatg ttataagcag gcttcaaacc tgtaaatttt
      121 atggaaagag atgacaaatg gctgatattt tttctaacgt gctggagata aggtgggaaa
      181 tggtcttgat gtgggtcata ggcgcattgc ttatatggct tgctatttcc aaagatatgg
      241 aaccgacttt actgcttcca atgggatttg gagctatact tgctaacctt cccacatcag
      301 gagctataac tcaggtgatt aacggaaata cagaagaagg cgtactggat gtcttattca
      361 atgcaggaat tgcaaatgaa ttgtttccgc ttcttttgtt tattggaata ggagcgatga
      421 tagattttgg tccgctactt tcaaatccca aaatgctttt gttcggagct gcagcgcaat
      481 ttggaatatt cttcactttc gctttagcag tattgctggg gttcgatttg aaagatgcag
      541 catcaatagg aataatagga gctgcagacg ggcctacatc aatattcgta gccaactact
      601 ttaacagtaa ctacatagga gcgattattg ttgccgcata ctcgtatatg gcattagtgc
      661 caataattca gcctcctgtt ataaggcttt ttactacaaa gaaggaaagg cttataaaaa
      721 tgccttatga acagaagaat gtatctacca cagtaagaat actctttcca ataattacaa
      781 cagctatagt cggtatattg tcaccaaggt ctgtagcttt agttggattt ttaatgtttg
      841 gtaatttatt gagagagtgc aaggtgttga ggcttctatc agaatcagcg cagaaggaac
      901 tggcaaacct tgtaaccata ttgctaggaa taacaatagc atcaaagatg agatacgatt
      961 tatttgtaag ggttgatacc ttgataatta tacttttagg actagtagca tttacgtttg
     1021 atacaatagg cggtgtgctg tttgcaaaac ttataaatct gtttgccaag aagaaaatta
     1081 accctatggt aggagccgca ggaatatcag cattccctat gtcttcaagg gttgtacata
     1141 agatgggatt gaaggaagat cctaccaact tcttattaat gcatgcagcc ggagcaaatg
     1201 tagccgggca gatagcatcg gtagtagctg gaggagtaat gatttcgctg gtcgagaaac
     1261 tgatataggg gaggtgctaa atgtttaatc gttttgattt agataatttt ctacagacac
     1321 caagagtggt tctattagga atgttaggag tatttgtagc gcttctagtt atatatgttt
     1381 caatactttt actaaacagg gtatttaggg ataaaaagaa aaaagattag atgtgtccaa
     1441 agattaaaag accaaatgaa taagagagca cattagaaag gaaagtacaa agaaacagct
     1501 taagggactt tccttctatt tttgtaaaca tatgatattc aacaaatata gcagatacct
     1561 ccagtatgta tagtataagt atgattacag tcctagaaaa aaacacagaa gaaaaaacga
     1621 ctgtaagatt aataaaaagg ttagtcataa tatttataag cagaaatact atccagaagc
     1681 tttttttcct ataaccgaac agaaaaaaga tgatgctttc aaatgcaagg gtaagcgcta
     1741 cgctataaac aagtcccatt actttttctc ttcagtctta ttctgcatat ttttcctttt
     1801 taagaatctg aacaaaagta ttccaattac tatcagtacc aacccagcta atacaaaagg
     1861 ccagtagatt attgggtaca gtattaccgg agttactaca tcacatatta ggatattcat
     1921 acatttatac tccctttctt cattattttc actgctatat acaatagaat tgcattggac
     1981 atgaatatta acataattat aaagttgcta agctgtaatc ccaagcctac tgccatacca
     2041 gggaataaga aggatgcagc aagaccaaaa gcaaatcctg gataatcagg cagcattcga
     2101 tatagcatat ataaagttag aggcatagaa caattaatta gaaactgacc cactaatgag
     2161 ggtacaatgc ttgaagaaag aaaagctata accggacctg atataagaat agataatata
     2221 attacaggta ctgctttaat tttatcaaga ataaaacctc cagcggcttt tcccatcatt
     2281 acaaaaactc ctaaaagtat agataaagtt attgtatcct tccatgggta actaatagaa
     2341 taggaagcca ttgaacgtac cgatactgaa attaggataa ttgctattat aatgagagta
     2401 tatacttttt tacttatacc actcatagaa aaagtggagg aatcttttga aggttcaatt
     2461 gactggtatt ctttcatcca gattaagcct atggaaatca agaaaagagc tatacttaca
     2521 taaatcataa tagaagggaa aagcattcca agaagcaaac ccatactccc aggtgctaca
     2581 aatatgccta aaggggcagc tttatttgaa gatcctttga gtactgctgc gccacctcca
     2641 acatgaaatg cgctgttacc aatacctatc agacaaacct taagcggaac tggcattagg
     2701 agcaaagctg ctaaggcggt cattaatcct cccgctattg ctatatgacg gtatcttttt
     2761 ataacatcag ccataatacc tgttataccc tgagtagaaa aggctaaaag atcatacatt
     2821 aatatgtatg gaatcatttc ctgcgatgag aaaatcgcac ttgcacatac tgcatctgtt
     2881 aaaaagtgag ctatagagta agctataagg gtcatcttcc gccccttcct gctcctcctc
     2941 ctcgtgatac tcctccgccg ccactatgga agcctcctga ccttcctcca aatgatgaac
     3001 ctccaccaaa tgaagatgta ccgcggaaag aagaactgct tctattaata gtattatgat
     3061 tattattaag attggaaaaa ctgctgttat tcttgttttt gtttaacaga tacatagcac
     3121 caagaaaagg ccagatatta ctattggaac tagatgatct ggtctgtgtt gaagagtatg
     3181 ttctttttgt actctttctt gttccgaata ttgatataag tactgcaata attactatta
     3241 ttataaaaaa ggtacgaaat acatcaaata tgtttataga tgtatcaggt ccgtaatagt
     3301 attcctcagg ataatactca tactcgttag gatattcaac aggagtatct attgctgtat
     3361 catgaactgc aagcatctct tcataaaaag cattgaaaac gcttaaaacg cctgcatcat
     3421 attcctgctg agcgaaataa ggttccaagt attcatataa aaggtatgag atggttccgc
     3481 ttgtaaaggt tctttccagt cctgctccct gcagagcata gtaatcgtca tctccaattg
     3541 agagcagaag cagaagaccg ttattattgt cttttgaacc tattccgtac tcattaaaca
     3601 gctcatatgc ataatcagca atggaagcag tatttgtaaa ctctactgtt acaaccacta
     3661 tctgtgctcc agttttgtca tataaagctg catttttatc aattatgagt ttcttagtct
     3721 gctccgataa aacatttgca gtatcattaa cataaaaatc cgaggtaggt tcgggaatat
     3781 ctgcagcaaa tactacatta caggtaagca ctaatattaa ggtaatgatt atcagtattt
     3841 ttttcatttc atatattaaa cgatgttaaa tagcagaagt ttcatctgaa ataatctgcc
     3901 ttttctatat tagtcaatgc agatattata ctagctggaa acttgtccag ttcttcatta
     3961 aactcatcta cattcttatt aaaatcgctc cttctcaaaa tgtcatctac agcaaggata
     4021 tcagcataat tgcttcttct taatctgtca tctgttgacg taagttccat tgtacctaaa
     4081 acatcatata tagctgatgc ggcatttgtt atatttacgc ttgcaacata tttttcttgt
     4141 gggttttcag cttcaagaag catatttctg gcctttgaca tgtcggtaat gtatttactg
     4201 ttactgtcaa gatatcttga agctattcct gcaaggttgt cagcatagga agacttagta
     4261 tttaaatcat tatccattgt gtcataaaag gaattgagca ccttcttttt ttgagaagat
     4321 aatgagacat aaccgcttat gagaacagaa gttatcataa tgactattac cagtatccag
     4381 gccagcgtgc ggttttcctt aaatttcatc aattcttgaa ttcaagcagt ttgtttttga
     4441 gctgctcttc aattttattc agttcagctt cagcttcttt tctctttgtt tttccttctt
     4501 cctgaatacg gagtatctca tcaagagtat tgataaggct ttcgttagta gtccttaatg
     4561 tctctatatc tacaatgccc cgttcagatt ctttagctat gtctatactg ccttgtttta
     4621 agctttcagc attctttaac aataactcat ttgtcatatc ggtaacagca cgttgtgcct
     4681 tcattgcatt ttgagaatga gttatgctca atgtcagaac catctgattt ttccataaag
     4741 gaatagtgtt tataagtgtt gtctgtattt tttcagttaa caggttatta ttgttttgaa
     4801 tcaatctgat ttgaggagcc atctgaacag caatatttct tgtgatttca aggtcatata
     4861 tcttcttttc aaccctgtcg agcatatttg caaaatcatt agcagcctgt gcatcttccg
     4921 gcagttgaga ttgtttagct ttatctctta attcaacaag ctttgtttct cttaattcat
     4981 tcaactttgc ttttccgcct tcaatataca gagcaagatc cttgatatag tcaagattca
     5041 tttcatacat tttatcaagc atggtaatgt ccttaagtaa aactacctga tgatcttcaa
     5101 gtacggctac tattttactg acattggctt ctgccttggc ataacgtgct tttaagacat
     5161 ctatatatct ttttgttttc ttgaagattc tttcaaaaaa gccgccactg tcctgatctg
     5221 cagtaaagtc tttaagttgt gccatcaacg aaccgatcat gtcaccaatt tctccaagtt
     5281 ccctggaacg taccttttca agggtagcat ccgaaaaact ggtaattttc ctttgtgcat
     5341 cagcaccata ctggagtatt gaagcactgc tcttaatgtc cagtttctga ccatattcca
     5401 aagccagttc tctgtctttt tctgttaaag ttatctcgct catgatagtc gtcctttcag
     5461 ttattttaag ttttccgctt tcagcatact gtcaaaaaca gtaatctcag cgtctatatc
     5521 catagcctca tcggcatata ggttatctaa atgtttatgg aaggactcta ttataccgtc
     5581 cattgcgcct tctatatcat tcttgatctg gtttatattc tctcctttgt tatcctgtgc
     5641 ttctgctctt gcatacgaat tgagaagttt aacagtagtt ggaagaaaat atgtcagaaa
     5701 tcttctgatg tcctttaact tttccggttt ttcggaaaca tagttgaata ttttttttgt
     5761 tatttcactt aaagagtcaa ttttattaga tattaaaggg ctttctatca gctcattagc
     5821 ttttctcata tcttcaagat acttgtttcc ttcttcgata gcctggtcta tttccttagc
     5881 acctgtatat ttaatggttt cttcaactat ttcaactctg tcagggaatt tttttcttaa
     5941 tataagatat gctgctataa acagcgataa agcaattata atatgaaaaa gtttgtgcat
     6001 agagaaaatc aaacaatata gtacccatac tgcacccgct agatatatcg gcatagctga
     6061 tttgtgtctg atttccataa aacttccttc tttcaaatag catctagatt atatcatata
     6121 aatcaataaa tggaataata tatggaacga tacagaataa aaattcatca gatgaatttt
     6181 tgaaaatccg ttgacacaag atttcacagt gatacaatat ctagtacaac atatagatta
     6241 tggaatacaa aatatagatt tatgggggat tagcgatgct tacggaaaat tcaataaaag
     6301 tactggaaag aaggtacctt aaaagggatt cacaaggcaa tattacggaa acttgtgatg
     6361 atatgttcag aagagttgct aaaacaatag catcagctga ttcagagttt ttaggcaagg
     6421 aagacatcgc aaagactgaa aaagactttt atgacatgat ggttaatttg gagtttttgc
     6481 caaattcccc tacattgatg aatgctggac ggactttagg tcagttggca gcatgttttg
     6541 tgcttccaat agaagattct atggaaggca tttttgaaac tgtaaaaaat gcagctctta
     6601 tacataaaag cggaggagga acaggtttca gtttttccag aataaggcag aatggagcag
     6661 ctgtaagttc tacaggaggt atagcctcag ggccgataag cttcatgaag gtctttaaca
     6721 ctgcaacaga agcggttaaa cagggaggta caagaagagg tgctaacatg ggtattctca
     6781 gaattgacca tcctgatata aaagcattta taacatgtaa ggaaaacagc actgaactta
     6841 ataatttcaa tataagtgta ggtttaactg agcagtttat ggaagctgta gaacatgaca
     6901 gtacatatga gcttatagac ccgtctacaa aacaggtagt aggagttgaa agcgcaaaag
     6961 aaatatttga catgatagtg cagcatgcat ggcttaacgg ggagcctgga attgtatttt
     7021 tggacaggat aaataaggta aataccgtac ccggactggg agaaattgaa agcaccaatc
     7081 cttgcgggga acaacctctg cttccatatg aatcatgtaa tttaggatca attaacctat
     7141 atactatgct taaagaagat aaaagcggat ttgattatga aaagcttgga agatgtgttg
     7201 taaaagctgt tcactttctt gacaatgtaa ttgatgttaa tgcataccca aatgaaaaga
     7261 ttgaaaaaaa ctcaaaagct acaagaaaaa taggccttgg aataatgggc tgggctgaca
     7321 gcttatatgc aatgagtatt ccatataact ctgaagaagc tcttgattta ggttatgagc
     7381 ttatgaaatt tataaatgaa gaatcaaaaa aagcatcaat gagccttgct gaaataaaag
     7441 gtgtatttcc tgcatatgat aaaagcatat atcctgcaca ggatataaag gtaaggaatg
     7501 caactactac aacaatagct cctacaggaa cattaagtat aattgcagga gtttctagtg
     7561 ggatagaacc ggtctttgca atttcatata tcagaaatgt tatggataat actgaaatgg
     7621 cagaagttaa ccctgttttt gaacagattg ctaaagaaag aggcttctat agtgaagaac
     7681 ttatgaaggt tatagctaaa aaaggctctc ttgctcatat agatgaaata cctgaggatg
     7741 taaaaaaagt atttgtcacc agccatgata ttgaccctat atggcatgtt aagatgcagg
     7801 ctgtttttca ggaacatgtg gataatgctg tatcaaaaac tgttaattta agaaatgaag
     7861 cgacacttga tgatgtaaga gaggtcttca tgcaggcata tgctatggca tgtaaaggtg
     7921 taaccctata tagggatgga agccgtgctt ctcaggtatt aagtgtttca aagcaacctc
     7981 caaaagaaga acatacagct tatgttactc caaaaccaag accgagaata actaccgggc
     8041 ttacagaaaa gtttaaaaca ggatgtggaa acctctacat aacagtaaac tatgatgaga
     8101 atggtgaaat atgcgagata ttcacaaaca caggcagagc aggcggatgt ccgtctcagt
     8161 cggaagcaac agcaagacta gtatctcttg cattaaggtc agggcttgac acaaaagaga
     8221 taattgacca gttaaaaggt ataagatgcg tatcaacaat cagacataaa gatcttggta
     8281 tattatcttg tcctgatgca attggaagaa tgatagaaaa ggttgcaaaa atgaatggcg
     8341 agttaaaaga agaagttaaa aggaccatcc catacattca atacggaaag gaaaaggata
     8401 cacctgtagt taaaaaagaa cagaaaacaa cagacagcag accttgtcct gaatgcggga
     8461 atccactgga aagtgatggt ggatgtgtta tttgcaggtc ttgcgggtac tcaaaatgta
     8521 actaactgtt aaaatcatac aaataggtgc acataaattt atggaatatt tttatagaca
     8581 tttttattta aaatagatat aattaacaca ggttaacacg aacaggagga tctatacatg
     8641 gcaaacaaaa aagagcatta tgtattagca gttagagatt tagataaagc attagctgat
     8701 atagcttcag gtaaagttaa aatgcccgtt gataattcaa aatatgcaga gattttttcg
     8761 aacatcgtaa gaagatgtag taatcttgaa gaattaaaca aatttatcag acagaacaaa
     8821 atgaagaaaa acgaatgcat ccattggtgg gaaggcattc ttgatgacgg atatgagctt
     8881 attacagttc agtacaatgc tcctgatgag aactttgttg aattagcagg aagcgaaaat
     8941 ctcattaagt acgttacttc agtaaaaggc taagaaaaac agtttacaac agaaagcatc
     9001 attaaacatg gtgctttttt tgttacgcaa ttttatgcta atatttattg ttgaagtcat
     9061 ccatctgttt atagtaacga ctttcttttg aaaaactcct aagtttttta atattaaata
     9121 tccataagaa aattacgaat ggtataaaca caaataacca gttaatattt agcgaaagnn
     9181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnntg ataagaaact acataaacgg
     9301 aagctctctt taatgattct tcagtaaggc cagcaacaat gaatgatgta tatgctattc
     9361 ctatcagagt ttctggaaaa atgttgaatc tttctagaaa tccttctatc aggatagccg
     9421 gtatactgat taaacaccct gctataaact gcttaataag aagtttagcc ggttctttgt
     9481 catgtctgtc aactaccaga agaagtataa taagtgcagc gactggtgat agtgcagaaa
     9541 aaattattct aataatatca gtcatcaggc ccccctaaaa cttgaagatt attcccacaa
     9601 ttcctgcaaa gacaataaat atgattggat gtattcttct ataaaatatt cttgctacaa
     9661 gaagacaaac aaataatata agcgccttta tgtcaattat atcaagaata ttgcgtgttt
     9721 caacaaatgt ttcaaatgta aaaacagaaa ccttcaatag ggcaaaggct gctgccgtta
     9781 taagtcctaa tgaagctggc cgaagtccat acattgcatt gagtactctt ctgtcctgac
     9841 tatacgaaac taaaagcttt gacagcagga taacaattat tacagagggt aatattacgg
     9901 aaagagaagc aattaaagat ccaagcattc cagcagccat ataaccggta tatgtagcca
     9961 tattaatacc gattggtcct ggagtagatt ctgagatggc tatcatatca ggaataattg
    10021 aggtatcaag ccagtcatat ctttcgccta tgtcatatag gaaaggaata gttgctaatc
    10081 ctccgcctat ggagaaaagg cctgttttaa aaaattccca gaagagttca agatatatca
    10141 tttgtccctc ttctttcttt cactcattcg tgttccaagc agaagtccta ctactgcaga
    10201 aacaagaact atgaatacag gagatattga agtaaacaca gaaataacaa acgagcctat
    10261 atacaaaatt atgcagaccc agtcaacaat agatttttta tacatatcta ttatagttac
    10321 aattacaaga gctgcaacag ctgctcttat accagcgaat gcatgattaa cagcttcaat
    10381 gtgtaaaaag ttagtaagca ctgttgcaat aatggtgatg ataataattg agggagttat
    10441 tactccaagt gcgcagaaaa taccgcctaa atattttttc ctgttataac ctatgtatgt
    10501 agaaacattt acagctatta cgccaggtga acactggctt aaggaataga aatctataag
    10561 ctgttctgta gcaagccatt ttctcttttc gactatttcc ttttgaagaa taggcagcat
    10621 cgtgtaaccg ccaccaaaag taaaaagtcc aacttttaag aaaatccaat atagttcaaa
    10681 aagggctctc ataaatcctc ctttcctaat tatatcttgt ttctggtcaa aaagtaagtt
    10741 tcagagcaaa gtaagttcgc aatttaacag tatacataaa gcatacaagg tataacttaa
    10801 tgcgaagcag tgtatttctt tttttgcgct gataaaagta atactgatgt cttattcaaa
    10861 tattatttca atctacttat agttgctata ttgcccataa tggtatgatt ctatgtttat
    10921 ctgttaaagg taataactct tgtgaaaggc atattacacc accttctgag atatttatac
    10981 cttttataga gtctagtatt tcgaaattct ttatatcctt caaagtagga caagtgtgtt
    11041 ttttaatttc aataggaaat aatgtgttat cctgaaatat tagtaaatca atttctctgc
    11101 cgttactatc gtaaagaaaa tagaaatttt cttcctttcc tgcgtttgta taacttttta
    11161 acagctccgc aacaacaaaa gtttcgaaga aatgtccatt cattgtacct tccacaagag
    11221 tatcgggtgt aagccagcga gttaaatatg caacaaggct tgaatccaga aagtaaagtt
    11281 tgggagtttt aaaaactctt tttattgagt tatttgaata tggtttcaat aaatatacaa
    11341 ttccgcttgt ttctaaaatt gaaagccagt gctttgctgt tggttcgctt atacctacat
    11401 ctctttctat atatgtctct ttatatacat ggaaattcct tcgacttatg ataaactaac
    11461 attgaatatt
//
LOCUS       DAOSXM010000066        11313 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2111
            NODE_3641_length_11313_cov_10.531888, whole genome shotgun
            sequence.
ACCESSION   DAOSXM010000066 DAOSXM010000000
VERSION     DAOSXM010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864178
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 11313)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11313)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:24:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,261
            CDSs (total)                      :: 2,229
            Genes (coding)                    :: 2,218
            CDSs (with protein)               :: 2,218
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 3 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11313
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3641_length_11313_cov_10.531888"
                     /isolate="AMR_MDS_2111"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            118..1047
                     /locus_tag="PLQ68_08340"
     CDS             118..1047
                     /locus_tag="PLQ68_08340"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HXK72702.1"
                     /translation="MNADYFSIKGKDGAFFGYMPHHVEDPEYIIVISPGLAEHAARYN
                     RLSSFLKNHNVQLYCIDHIGQGIYATNLGVWPVNGFEICVNNMKSLIDYAHENHKNKK
                     IVLFGHSMGSFMSLSYIEQFASNIDACILSGTNDQQHPLVLKGGKFIAMLQELFIGRN
                     KNSKILNSLSFGQFNKQFAPNRTEFDWLSRDEKEVDKYIQDPLCGYVPSVGLFKELFK
                     ALSTIYKDEMVIKIPKDLPIHLMSGANDPVGGNSKGPLSLLARLEKSKVSNITVQIFD
                     DMRHECLNEIGNDVVMKHVHDTCKNLLSLTDNT"
     gene            complement(1030..3147)
                     /locus_tag="PLQ68_08345"
     CDS             complement(1030..3147)
                     /locus_tag="PLQ68_08345"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HXK72703.1"
                     /translation="MSITEKDKIYEESRLKDAYDKLIKRIEDLEISLNDSLIRFKKSN
                     EQMWDNGKRSLVDFSDAIENMPYLDSIRSDFYKVETLKKELKRMLLLQRDMYFGRIDF
                     QEESEDEVEKIYIGKFSFTDDFGQYVVFDWRANICSLFYENEIGRVYYDAPDGRIYGD
                     IFRKRQFEILHDKIITMFDSSIMIDDSILKEILSKSKDSKMGNIVETIQKEQNQAIRS
                     TYNIIIVDGPAGCGKTSIAMHRAAWLMYKYKNELKNNQIIIFSPNDTFNDYVSEVLPE
                     LGEDNISMSTFNKLAYKILSDRKILISKYTELEDVLLDNEYIEKSDIKLKYSIDFARK
                     LKEYMGLISKGNYPFKDFYINNELILSKKDFENLFYQEYEFNDVITRLSKIRLMLNDR
                     ISPIIRNLRMQYVKSVKDNSIDMREGFVKLKEDCKNVYQSVDMAFAADFEMIYAAFLK
                     HTYGQNVSSRFLDRLDNGYIYAEDTAPLIYVRLLLGQRHSFNDIRHLIIDEFQDYSYI
                     EKIIISILFKNCHMTLLGDANQKINYYIENAQDNEIFNNARLIKLDKSYRSTYQIAKY
                     CNDMLKDNIDIKYFNRYGNEPEIINTKEINPEELSEIIINKVEQYRKMGYRSIALITR
                     TEAFAKELEPYLKEAKISRVTQKNTSYTVGSVIMPSYLTKGLEFDAVIVMDTSNDIFK
                     KEEELNLFYTCCTRALHELCIIS"
     gene            complement(3257..3829)
                     /locus_tag="PLQ68_08350"
     CDS             complement(3257..3829)
                     /locus_tag="PLQ68_08350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013280894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HXK72704.1"
                     /translation="MVKSIVIAGVGGQGTLLASRLIGEAATSKGYDVKVSEVHGMAQR
                     GGSVITYVRYGDEVISPIIPLGEADVLIAFEQLEALRWLKYLKKGGLLISSTQKIDPM
                     PVITSKMEYPDDIVEVLSEKVENCIFIDALSLAVKAGSTKAVNSILIGRMAKDSDIDK
                     DTWIKALKKIAPLKFIDLNIIAFELGYNFS"
     gene            complement(3829..5571)
                     /gene="iorA"
                     /locus_tag="PLQ68_08355"
     CDS             complement(3829..5571)
                     /gene="iorA"
                     /locus_tag="PLQ68_08355"
                     /EC_number="1.2.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014313331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043805 - indolepyruvate ferredoxin
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HXK72705.1"
                     /translation="MKQLLLANEAVARGLYEAGVKVVSSYPGTPSTEITEAVSKYKEI
                     DSEWAPNEKVALEVAATSAIAGARSFCAMKHVGLNVAADPLFTMSYIGVNAGLLIAVA
                     DDPGMHSSQNEQDSRHYAKASKIIMLEPSDSQEAKDFTKVAYDISEQFDTPVLLRLST
                     RVSHSQSLTSFEERQEKPLNSYVKNTQKNVMMPAMARVKHEIIEKRIEEQKEFAYSSN
                     LNKMEIKDKKVGFISSGIAFQYSKEVMPDASYLKLGISYPLSEKLLKEFASQVEKIYV
                     IEELEPIIEETCKAIGINVIGKEILPITGEYSVDLIRNRIFGNEPFNYLSDYDMSKVP
                     LRPPVMCPGCPHRGLFYALKKLKLTVTGDIGCYTLGALDPLSSIDTSICMGSGISSLH
                     GFNKARGNDFMKKCVGVIGDSTFVHSGITGLIDIVYNRGVGTVIVLDNSITGMTGHQN
                     NPMNGLDISGNATTQIDIELLAKSIGVRRVKVIDPFNIKESISVIKEEVETMEPSLII
                     ARRPCALLKTVKYTGKVYVNREKCIKCKACMSIGCLAISFKNNQIEIDESLCNGCGLC
                     INVCKFNALVKKEE"
     gene            complement(5546..7021)
                     /locus_tag="PLQ68_08360"
     CDS             complement(5546..7021)
                     /locus_tag="PLQ68_08360"
                     /inference="COORDINATES: protein motif:HMM:TIGR00813.1"
                     /note="Members of the Solute:Sodium Symporter (SSS), TC
                     2.A.21 as described in tcdb.org, catalyze solute:Na+
                     symport. Known solutes for members of the family include
                     sugars, amino acids, nucleosides, inositols, vitamins,
                     urea or anions, depending on the system; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/solute symporter"
                     /protein_id="HXK72706.1"
                     /translation="MVKIIIIAIFIGIQVAVGIYSTKKINSVDDFLLGGRKMGAWLSA
                     IAYGTSYFSAVIFIGYAGGIGWEMGTSAIWIGIGNALFGSFLAWKILAKRTRSVTREL
                     NAKTMPEFFQKRYNSDLLKILASVLTFVFLVPYTASVYKGLGYLFAKLFGASVTINQA
                     YIISVTIMALFTVLYLVLGGYIATAMNNLIQGIIMIVGTIIIVIYVINSPNVNGLSQG
                     LDSLNNIKEGLGTIIGPNPWKLLSLVILTSFGVWGLPQMVHKYYAIKDDSAIKRGTII
                     ATVFAAIIGIGAYFTGSFGHLFFSEIPNGVADTIVPEMLTLVLPDVFLGIFMVLILSA
                     SMSTLSSLVLVSSSSITVDLIKGYFKKDLNEKKQVALLRIFCVVFVLISVIIALTNNN
                     TIVTLMNLSWGTLAGCFLGPYILGIYNKRTNKISALVGMLSGFTTMMTLVIINGMTYS
                     TIGGCISMAVSIVVTYIASYIFKDKEFKTQEIKDEAIVTSK"
     gene            complement(7074..8507)
                     /locus_tag="PLQ68_08365"
     CDS             complement(7074..8507)
                     /locus_tag="PLQ68_08365"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006572906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HXK72707.1"
                     /translation="MSTDIVNNYTLLCDFYELTMANGYFKTGYKDKICYFDMFFRNNP
                     DNGGFAIACGLDQVIEYIQNLHFSEEDIAFLRSKNCFDDNFLEYLKTFKFNGDMYAVP
                     EGTIVFPNEPLITVRAKAIEAQIVETFILLTINHQSLIATKANRIVRSSKGRPVMEFG
                     ARRAQGTAASVLGARAAYIGGCAGTSCTLAEKMYTVPAGGTMAHSWIQMFDTEYEAFM
                     AYAISYPDNCTLLVDTYNVLKSGVPNAIKVFKELGITKCAIRLDSGDITYLSKKARAM
                     LDEAGLVNCKIVASNSLDEYIIRDILMQGACLDSFGVGERLITSESDPVFGGVYKLVA
                     VEDQDNCIIPKIKVSENPTKITTPHFKKVYRIYCNDMKKAEADLICVHDEIIDTEKPL
                     TIFDPDYTWKKKTYENFSVKELMVQIFKDGECIYDRPSVKTIRKYCEKQIAEHWDEVK
                     RFENPHNYYVDLSEKLWNIKHNMLNKD"
     gene            8616..8701
                     /locus_tag="PLQ68_08370"
     tRNA            8616..8701
                     /locus_tag="PLQ68_08370"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8649..8651,aa:Leu,seq:caa)
     gene            complement(8754..10664)
                     /locus_tag="PLQ68_08375"
     CDS             complement(8754..10664)
                     /locus_tag="PLQ68_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015261389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72708.1"
                     /translation="MKKLLLILMTAVLALITLAGCNGQKAVVENGTIVMEGISVDFGG
                     NNLTDKKSASLKTVKSVKTEKDNGLSSELYELTLDKPYTNPVTVSLPIPSEFSGKDNE
                     ALLLGIGVECEYDSGAKGIEYFYFPAEVDDKTANASFLPSDLSKATLYMGSSQGSATA
                     IKNTVFTLGLFTSEIHYEDNGHFYLYYPTKVNGKFFYGIVGSDGLKDILYDLEATYEK
                     FKALGYKYDESDFPMNVHIKKIEDEGSYHALFGDITLNVTNFKDKYVSGSLNPLLWHE
                     FFHYVQGCYTGIFSSTEWIDEATSSYYEASANNLTYTSLTTQYYERQFISALPEDDSA
                     ADGYARSPLISFLVKKKGSEEWIKTLYENGGNKEAFISAAGEISGWSHDYYTALASGQ
                     VGFEGASIIYKNVKDGVYGPEVGASIKLAIPDENEQKQMQGEDEIILGSVSVAMQGQG
                     SRFIAITVSSDELKKLPDGVEPEVECEGAKITVLSLAGRNVNNCGKVLLNLKDSIDNN
                     KVYLVVVTSKSAANVHGDFDIKIKMSNKKVDFSGTYKGVLNVIETDADIDVTTIVTFE
                     KEFGDGSYYNIVCSNDETQSKYINGSYFVRANGEANIAGAVFQFSSDGMSFTASMMDF
                     ANKTWGTISAYK"
     gene            complement(11014..>11313)
                     /locus_tag="PLQ68_08380"
     CDS             complement(11014..>11313)
                     /locus_tag="PLQ68_08380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72709.1"
                     /translation="GLDCIQMDQQENMGLDYLGSKYKGKITFFCPVDIQGTFAQGTLE
                     EIKQYCHRMFHALGSSKGGFIAKWYQDPKGVGHRPEAIDAMSQEFLKISDDIFSK"
BASE COUNT         3671 a         2148 c         1500 g         3994 t
ORIGIN      
        1 tcaaatggga tttctctttt attttatcaa tccccaaaca agtttacact atcgatgata
       61 tatatttctt ggataattta tattaatgtg ctatcattga tatcgggagt gaataaaatg
      121 aatgctgact atttttcaat taaaggtaag gatggagcat ttttcgggta tatgccccat
      181 catgttgagg atcctgaata tataatagta atatcacctg gtttagcaga acatgcagca
      241 agatataaca gattatcttc ttttctgaaa aatcataatg tacagcttta ttgtatagat
      301 catattggtc aaggaatata tgcgacaaat cttggcgtat ggccagtaaa cggatttgag
      361 atatgcgtga acaatatgaa atctttaata gattatgcgc atgaaaatca taaaaacaaa
      421 aaaattgttt tatttggcca tagcatgggt tcattcatga gcctttcata catagaacaa
      481 tttgcatcaa atattgatgc atgcatttta tctggaacta acgatcagca acatccttta
      541 gttcttaaag gaggaaagtt tattgctatg ttacaagaac tatttattgg cagaaataaa
      601 aattcaaaga tattaaactc tttatcattt ggacagttca acaagcaatt tgcgcctaat
      661 aggacagaat ttgactggtt atcacgcgac gaaaaagaag tagataaata tattcaagat
      721 ccgctttgtg gttatgtacc aagcgttggg ctgtttaaag aattatttaa agcattatct
      781 actatttata aagatgaaat ggtaatcaaa atcccaaagg atttgccaat tcatttgatg
      841 agcggagcta atgaccctgt aggcggaaat tccaaaggcc ctctttcatt attagctaga
      901 ttagaaaaat caaaagtttc aaatataact gtgcaaatat ttgatgatat gagacatgag
      961 tgtcttaatg aaatcggtaa tgatgttgtt atgaaacatg tccatgatac atgcaaaaat
     1021 ttactttcac taactgataa tacataattc gtgcaaagct ctggtacaac atgtgtagaa
     1081 gaggtttaac tcctcttctt ttttaaatat atcgttagat gtatccatta ctattactgc
     1141 atcaaattcg agtcccttgg taagatatga aggcattatt acggaaccga cagtatatga
     1201 agtatttttt tgtgtcactc tgcttatctt agcttctttt aaataaggtt ccagttcttt
     1261 ggcaaatgct tcagttcttg taatcaaggc tattgaacgg tatcccatct ttctgtattg
     1321 ctctacctta ttaattataa tttcagataa ttcctcagga ttaatctctt tcgtattaat
     1381 tatttcaggt tcattaccat atctgttgaa gtattttata tctatattgt cttttaacat
     1441 atcattgcag tatttagcaa tctgatatgt agatctataa cttttatcta gtttgattag
     1501 tcttgcatta ttaaatattt cattatcctg agcgttctct atatagtaat ttattttttg
     1561 gtttgcatca cctaataatg tcatgtgaca atttttaaat aaaatagata taataatttt
     1621 ttctatatat gaataatctt gaaattcatc aataatcaga tgccttatat cgttgaagct
     1681 atgcctttga ccaagaagta acctcacata tatcaaagga gcagtatctt cagcataaat
     1741 atagccgtta tctagtctgt caagaaatct tgagctaaca ttctgaccat aagtatgctt
     1801 taagaatgca gcatatatca tttcaaagtc ggcagcaaaa gccatatcta cagattgata
     1861 aacattctta cagtcttctt ttaactttac aaagccttct ctcatatcaa tactattatc
     1921 tttaacactt tttacatatt gcattctaag atttctaata attgggctta ttctatcatt
     1981 tagcattaac cttatttttg acaatcttgt aattacatca ttaaattcat actcctgata
     2041 aaataaattt tcgaaatcct tcttactaag tatgagctcg ttattaatat aaaaatcctt
     2101 gaaaggataa ttcccttttg atattaaacc catatattct ttgagttttc tagcaaaatc
     2161 aatagaatat tttaatttaa tatctgattt ttctatatat tcattatcaa gcaagacatc
     2221 ttcaagttcc gtgtatttcg aaataagtat ttttctgtct gataatattt tatatgcaag
     2281 tttattaaaa gtggacatac ttatattatc ttcaccaagt tctggtaaaa cttcggaaac
     2341 ataatcgtta aaggtatcat ttggtgaaaa tatgattatc tgattgttct ttagttcatt
     2401 tttgtattta tacataagcc atgctgctct atgcattgct atggatgttt ttccacatcc
     2461 tgctggtccg tcaacaataa taatattata tgtgcttctt atggcctgat tctgctcttt
     2521 ttgaatggtt tctacaatat tacccatttt tgagtctttt gatttgctaa gtatctcctt
     2581 aagtatgcta tcatctatca ttattgatga atcaaacatt gttattatct tgtcatgaag
     2641 tatttcaaat tgcctcttcc taaatatatc accataaatc cgaccatcag gagcatcgta
     2701 atagactctt cctatctcat tttcataaaa caaactacat atattagctc tccagtcaaa
     2761 tacaacatac tgtccaaaat catcggtaaa actgaatttt cctatatata ttttttcaac
     2821 ttcatcttca gactcttcct gaaaatcaat tctgccaaag tacatatctc tttgaagtaa
     2881 caacattctt tttagctctt tttttaatgt ttcaacttta taaaaatcag atcttatact
     2941 atcaagataa ggcatgttct caatggcatc agaaaagtca acaagacttc ttttaccatt
     3001 gtcccacatc tgttcattac ttttcttaaa acgtattaaa ctatcattta aagatatttc
     3061 aagatcttca attctcttaa ttaacttatc ataagcgtct tttagtctgc tttcttcata
     3121 tattttatct ttttcagtaa tgctcataaa gaccccttta ttataatatg cattaaaaaa
     3181 tattatatct tattttaccc cagatagcaa attggatgtg ttgccacatc ctaataaatt
     3241 attaagtaaa tcaatactaa gaaaagttat aacccagttc aaaagcgata atatttaaat
     3301 caataaattt caaaggagct atttttttca aagcctttat ccaagtatcc ttatcaatat
     3361 cagaatcttt agccatccgt ccaatcaata tcgaattgac tgcttttgta gaccctgctt
     3421 taactgctaa tgaaagagca tcaatgaaaa tacagttttc caccttttca gaaagaacct
     3481 caactatatc atcaggatat tccattttag atgtaatgac aggcataggg tcaatttttt
     3541 gtgtactgct aattaggagc ccaccttttt ttagatattt taaccatctg agagcttcta
     3601 gttgctcaaa tgcaattaat acatcagctt ctccaagtgg aattattgga gaaataacct
     3661 cgtccccgta tcttacataa gttataacag aaccgccacg ttgtgccatc ccgtgtactt
     3721 ccgaaacttt tacatcatat ccttttgatg tggctgcttc acctataagc cttgatgcta
     3781 ataaagtgcc ttgtccccca acaccagcta taacaattga tttgaccatt attcctcctt
     3841 ttttactaat gcattaaact tgcatacatt aatacataat ccacaaccat tacataaaga
     3901 ttcatcaatc tcaatttgat tgttcttaaa tgatattgcc agacatccga tactcataca
     3961 agccttacat tttatacact tttctctgtt tacataaact ttcccagtat acttaacagt
     4021 tttcaataaa gcacatggcc ttcttgcaat tatcaatgat ggttccatcg tttcaacttc
     4081 ttctttaata actgatattg attctttgat attgaaagga tcaattacct taactcttct
     4141 tacgcctatg ctttttgcca aaagttcaat atcaatttga gttgttgcat tgcctgatat
     4201 atccaatccg ttcatgggat tattctgatg acctgtcatt cctgttatag aattatcaag
     4261 tactataact gtacctacac ctctattata tacaatgtct ataaggccag tgattcctga
     4321 atgcacaaat gtcgaatctc caattacacc cacacacttt ttcataaaat cattaccacg
     4381 tgctttattg aagccatgaa gtgatgatat accacttccc atacatattg aggtgtcaat
     4441 tgatgataaa gggtcaagag ctcctaaagt atagcatcct atgtcccctg ttactgtaag
     4501 tttaagtttc tttaaagcat agaacaatcc tctatgtgga catccaggac acatcactgg
     4561 tggccttaga gggacttttg acatatcgta atccgataga taattaaatg gctcgttacc
     4621 aaaaatcctg tttcttataa gatcaacaga atattcacct gtgattggca atatttcctt
     4681 accaattacg tttataccaa tagctttaca agtttcttct attattggct ctagttcttc
     4741 aataacataa attttctcaa cttgagaagc aaattctttt aataattttt cagacaatgg
     4801 ataacttata ccaagtttta agtaagaagc atcaggcata acttcttttg aatattgaaa
     4861 agcaatacca gaagaaatga atccaacttt tttgtcttta atttccatct tgtttagatt
     4921 agatgaataa gcgaattctt tttgctcttc tattcttttc tcaataattt catgctttac
     4981 tcttgccatt gcaggcatca ttacattctt ttgagtattt tttacatatg aatttaaagg
     5041 tttctcctgt ctttcttcaa aagaagtcag actttgtgaa tgacttactc ttgttgaaag
     5101 ccttaataag actggtgtgt caaactgttc tgagatatca taagcgactt ttgtgaaatc
     5161 ttttgcttct tgtgaatctg aaggttcaag catgattatc tttgatgctt ttgcataatg
     5221 tctggaatct tgctcattct gtgatgaatg cattccaggg tcatctgcaa cagcaataag
     5281 caaacctgca ttaacaccaa tatatgacat ggtgaacaag ggatctgctg ctacattgag
     5341 tcctacatgt ttcattgcac aaaaggatct ggctccagct atcgctgaag tagcagcaac
     5401 ttccagtgca actttttcat ttggagccca ttcagagtct atttccttat atttactaac
     5461 agcttccgta atttcagtgc ttggtgtgcc tggatatgaa gaaacaacct taacacctgc
     5521 ttcatacaat cctcttgcta ccgcttcatt tgctagtaac aattgcttca tctttaatct
     5581 cctgtgtttt aaattcttta tccttaaata tgtaggatgc tatataagtc acaactattg
     5641 aaaccgccat agaaatgcac cctcctattg tggaataagt catgccattt ataataacaa
     5701 gtgtcatcat agttgtaaag cctgaaagca taccaactag agcagatatt ttatttgttc
     5761 tcttattata aatgcctaaa atgtatgggc ctaagaaaca tcctgcaagc gttccccaag
     5821 aaagattcat aagtgtcact attgtattgt tattagtaag tgctataata actgaaatta
     5881 aaacgaaaac aacacagaat attcttaata atgctacttg tttcttttca ttcaaatcct
     5941 tcttaaagta tcctttaatt aaatcaactg ttattgagga gcttgaaacc aatactaatg
     6001 atgatagtgt tgacatagat gctgacagta ttaataccat aaaaatacct aaaaatacat
     6061 caggtaaaac caatgtaagc atttcaggca ctatagtatc agcaacacca tttggaattt
     6121 ctgaaaagaa aagatgtccg aatgaaccag taaaatatgc tcctattcca attatcgcag
     6181 caaaaactgt agcaataatt gtacctcgtt taatagctga atcatctttt atcgcataat
     6241 atttgtgaac catctgaggc aacccccaga ctccaaaact tgttaaaata actaatgaaa
     6301 gcagtttcca tggattagga ccaattatgg ttccaagacc ttctttaata ttattaagcg
     6361 aatcaagccc ctggcttaat ccattaacat ttggtgaatt tattacataa ataactatga
     6421 ttattgtacc aacaatcatt attatgccct gaataagatt attcattgca gttgctatat
     6481 aaccgcctaa tacaagataa agcactgtaa atagagccat aatagtaact gatattatgt
     6541 atgcctgatt tattgttact gaagcaccaa acagtttagc aaaaagatac cctaaacctt
     6601 tatatactga tgcagtataa ggaaccaaaa atacaaatgt taaaacagat gccaatattt
     6661 tcaataaatc tgaattatat cttttctgaa aaaactcagg cattgtcttt gcatttagct
     6721 ctctagttac agaacgtgtt ctttttgcaa gaattttcca tgcaagaaaa cttccaaata
     6781 acgcatttcc aatacctatc caaatagctg atgtgcccat ttcccatcca ataccacctg
     6841 catatcctat aaatattaca gcactaaaat aagaagtgcc atatgcaatt gcagaaagcc
     6901 atgcacccat ctttcttccg cccaacaaaa aatcatcaac actgttgatt ttctttgtgc
     6961 tgtaaatacc tacagcaacc tgtataccaa taaatatggc aataataata attttgacca
     7021 tcctaccatc ctcccattct actaaacgaa ttatatatta attattatat gtgtcaatcc
     7081 ttattgagca tattatgctt tatattccat aatttttctg ataaatccac ataataattg
     7141 tgtggattct caaaacgttt aacttcatcc caatgttcag caatttgttt ttcacaatat
     7201 tttcttatag tttttactga aggtctgtca tatatgcatt caccatcttt aaaaatctga
     7261 accattaatt ccttaacact aaagttttca taagtttttt tcttccatgt gtaatcaggg
     7321 tcaaaaatag ttaaaggttt ttctgtatcg ataatttcat catgtacaca tatcaagtca
     7381 gcttctgctt ttttcatatc attgcaataa attctgtaaa cttttttaaa atgaggagta
     7441 gttatctttg ttggattctc acttacttta attttaggta ttatgcaatt gtcttgatct
     7501 tcaactgcaa ccagtttata tactcctcca aaaacagggt cactttctga agtaataagt
     7561 ctctcaccta caccaaagga atctaaacaa gcaccttgca ttagaatatc tctaataata
     7621 tattcatcca aagagtttga agcaacaatc ttacaattta ctaatcctgc ttcatcaagc
     7681 atggctctgg ctttttttga taaataagta atatctcctg aatccagcct tattgcacat
     7741 ttagtaattc cgagctcttt aaaaacctta attgcatttg gtacgcctga cttgagaaca
     7801 ttatatgtat caacaagcaa agtgcagtta tctggataag agattgcata tgccataaat
     7861 gcttcatact ctgtatcaaa catctgaatc catgagtgag ccattgttcc tcctgctggt
     7921 acagtataca ttttctctgc taaagtacat gatgtacccg cacagccacc aatatatgca
     7981 gctcttgctc caagaactga agctgcagtg ccctgagctc ttcttgcacc aaattccatt
     8041 actggtcttc cttttgatga cctgactatt ctgttagctt ttgtagcaat taatgactga
     8101 tgatttattg ttaaaagtat aaaagtttca actatctgcg cttcaattgc tttagctctt
     8161 accgtaataa gcggttcatt aggaaaaact atagttcctt ccggaactgc atacatatct
     8221 ccattaaact taaatgtttt cagatattcc aaaaaattat catcaaaaca gttttttgat
     8281 cttaaaaatg caatatcttc ttccgaaaaa tgaagattct gtatatattc aataacctgg
     8341 tccagtccac atgctatagc aaaacctcca ttatcaggat tatttctgaa aaacatatca
     8401 aaataacata tcttatcttt atatcctgtt ttaaaataac cattagccat ggttaattca
     8461 taaaagtcac ataataaagt ataattatta actatatcag tactcatata acacctcttc
     8521 ctgctagtaa acatcatagc ttctttatta tatcattttt atataaatct gttgcataaa
     8581 tccaattata tatgttaata taacttaagt tatcagccgg cgtggtgaaa tggcagacgc
     8641 gctggactca aaatccagta taggcaacta tgtgtgggtt caagtcccac cgccggtacc
     8701 aaaaaaaacg cactaacaag tgcgtttttc tttttatact ttataatttt taactactta
     8761 tatgcactaa tagtacccca tgtcttgtta gcaaaatcca tcattgaagc agtgaatgac
     8821 attccatcac ttgaaaattg aaatactgct ccagctatat ttgcttcacc attagctctt
     8881 acaaaataac ttccattaat atatttactc tgtgtttcat catttgagca cacaatattg
     8941 taatatgacc catcaccaaa ctctttctca aaagtaacaa tagtagtcac atctatgtca
     9001 gcatcggttt caattacatt caagactcct ttgtatgttc ctgaaaaatc tactttctta
     9061 ttactcattt taatcttaat atcaaaatct ccatgtacat tagcagcaga ttttgatgtt
     9121 acaactacaa gataaacctt attattatct attgaatcct taagatttag taatacttta
     9181 ccacagttat taacatttct gccagctaaa gacaatactg tgattttagc tccttcacac
     9241 tcaacttcag gctcgactcc atcaggcaac tttttaagct catctgaact tactgttata
     9301 gctataaatc gtgaaccctg accttgcata gcaacagaaa cagaacctaa tataatttca
     9361 tcttctcctt gcatctgctt ttgctcattt tcatctggta tagcaagttt tatggacgca
     9421 cccacttcag gaccgtatac tccatctttt acatttttat aaattatact cgctccttca
     9481 aaaccaactt gtccagatgc taaagctgta tagtaatcgt gagaccatcc tgatatttct
     9541 cctgcagctg aaatgaatgc ttctttatta cctccattct catataatgt ttttatccat
     9601 tcctctgatc ctttcttttt cacaagaaat gaaatcagag gtgatctggc ataaccatca
     9661 gcagctgaat catcttctgg aagagcactt atgaattgac gttcataata ttgagtagtt
     9721 aaagatgtat aagtaaggtt gttcgctgaa gcttcataat atgatgatgt tgcctcatca
     9781 atccattcag tactactaaa aattcctgtg taacaacctt gtacgtaatg gaaaaattca
     9841 tgccataaca aagggttaag agaacctgat acatatttgt ctttaaaatt agttacattc
     9901 aatgttatat ctccaaacaa tgcatgataa gagccctcat cttcaatttt cttgatatga
     9961 acattcatag gaaagtcact ttcatcatat ttataaccta atgctttaaa cttttcatat
    10021 gttgcttcta aatcatataa aatatctttc aacccgtcac ttcctactat accataaaag
    10081 aattttccat ttactttagt aggataatag agataaaagt gaccattgtc ttcataatgt
    10141 atttctgatg taaaaagacc taaagtaaaa actgtatttt ttattgcagt agcacttccc
    10201 tgacttgaac ccatatacaa agttgcttta gataaatcag aaggcagaaa gcttgcatta
    10261 gcagttttat catctacttc agctggaaaa tagaaatatt ctattccctt tgcaccactg
    10321 tcatattcgc actctacacc tatacccaat aagagagcct cgttatcctt tccactgaat
    10381 tcactaggaa ttggtaaaga tactgtaact ggattagtgt atggtttatc tagtgtcaat
    10441 tcataaagtt cagaagacag accattatct ttttcagttt taacagattt aactgttttc
    10501 aacgatgctg actttttatc tgtaagattg ttacctccaa aatctacaga aataccctcc
    10561 ataacaatag tgccattttc cactactgcc ttttgaccat tacatccagc taaagtaatt
    10621 agtgctaata ccgctgtcat taaaattagt aataattttt tcaaaacaaa atcctcctgt
    10681 aataagtaat tgaacttcat taatgcgctt taaacttagg acatacttca ctgaaacaat
    10741 aatgcgccaa cttacataag attgattaaa agatagctat aactaaaatt ttctcaaaat
    10801 ttctaaatga tttaaaagca aaaataatct aaaatttcca tatgaaatta ctctactgtt
    10861 aatcatctgt ctgccattgg aggctattgt cccaaaactg ctttaatgag tcaattataa
    10921 tatcacatta taataatttc aattagtttt taatttttat tcttaattaa acaatattaa
    10981 ttatttatgt gttcataatg atgaaactta ctactattta gagaagatat cgtcactaat
    11041 ctttagaaac tcctgactca tagcatcaat agcttcaggt ctgtgtccaa caccttttgg
    11101 gtcctgatac cactttgcta tgaatcctcc ctttgaactt cccaaagcat gaaacattct
    11161 atgacagtat tgcttaattt cctctagtgt accttgtgca aaagtacctt gaatgtctac
    11221 aggacagaaa aaggttatct ttcctttgta cttactccct aaataatcca atcccatatt
    11281 ttcctgttga tccatttgaa tacaatcaag tcc
//
LOCUS       DAOSXM010000067        10819 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2111
            NODE_3842_length_10819_cov_10.574229, whole genome shotgun
            sequence.
ACCESSION   DAOSXM010000067 DAOSXM010000000
VERSION     DAOSXM010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864178
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 10819)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10819)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:24:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,261
            CDSs (total)                      :: 2,229
            Genes (coding)                    :: 2,218
            CDSs (with protein)               :: 2,218
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 3 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10819
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3842_length_10819_cov_10.574229"
                     /isolate="AMR_MDS_2111"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(150..650)
                     /locus_tag="PLQ68_08385"
     CDS             complement(150..650)
                     /locus_tag="PLQ68_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005962187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HXK72710.1"
                     /translation="MTVYTGKIEMQSKDHMPDFHNITDQVKKIVEESGVKNGICVVYS
                     HHTTCSVMTQECSHDTTFFGREHLQQDLINIMERIVPTCRYEGQYLHPGPKHIDFALT
                     FPDEEPKGSLNTDAHLRSCFFGRSETIVLTDGKLSLGDFGFIYFIDWDQVRERKRVAE
                     VQIIGE"
     gene            complement(668..1372)
                     /locus_tag="PLQ68_08390"
     CDS             complement(668..1372)
                     /locus_tag="PLQ68_08390"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HXK72711.1"
                     /translation="MYKGLKISAPFFEIGPKAYLYGENMLSLAKVIDKVAMKYDVDII
                     VTPQYTDISLLALNTSRILVFAQHMDYLPIGRGLGSVLPEAVKAAGAVGVMLNHAEKP
                     LTMEEIQKTIERADEVGLGTIVCSDTIEQMKQIAKMSPNLIVAEPTELIGTGQTSDIS
                     YVKESIETVKQINPEIMVLQGAGIKDGNDVYNVIKAGALATGSTSGIIKAKDPYAMVE
                     EMIYSLRRAWDEIHNK"
     gene            complement(1382..1978)
                     /locus_tag="PLQ68_08395"
     CDS             complement(1382..1978)
                     /locus_tag="PLQ68_08395"
                     /inference="COORDINATES: protein motif:HMM:NF013539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HXK72712.1"
                     /translation="MRSEILEAARKSLNIEKKEIERLISYLNEDSLLEAVEKLANCKS
                     IMTVASGSSGIAAKKFAHSLCCIERHAMFMSPSEAVHGGLGALDSDDVMVMVSRGGKT
                     VELLPIISVCNKKKATLIAVTENEESILARSSDIVLKLNIDKESDPLGLMATSSFVAT
                     IALFDSILAALIVKTGFTSEQFGIIHPGGAVGELLNKK"
     gene            complement(1975..3354)
                     /locus_tag="PLQ68_08400"
     CDS             complement(1975..3354)
                     /locus_tag="PLQ68_08400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72713.1"
                     /translation="MKRYMKGVNKMTKAAFIGFGEVNTPKELIIEKCRKAEDSLLKEG
                     IELISIYPVTDDYERSDVNKAIESLVSQSFQTVILCIAGWIPSHAVISITERFKDTPM
                     VLWGLSGWMEKDRIVTTADQAATSAIRKVMSDLGYKFKFVYEIVGKPLNSKKIADFCK
                     ASDAMFKLKSSRAGMMGYRDMNLYGTMFDGVKLKRHIGTDIECFEILEVEQRAVSISK
                     TRIQEVIHNEIEKWKFLKSCDSVTKEKAARYYLAISDICKERGYEAVSLKDVDGMKKL
                     LSYPPAPIFMLLSDIDKLCTIPENDSYGMVTQLMVKYLTGQCGAYLEFYEFMEDCVLA
                     GVPDYIPKEITQGDTVVIPAAFGQLSEGILNVSSVKPGNVILYRLSQNKEGYCMHIVK
                     GRAETADKWEEAGWTQPAPQLPGLMIYLDDVETFAMNVMSQHYIIAYEDNADKMVDLC
                     KLLNIEVVR"
     gene            complement(3326..4735)
                     /locus_tag="PLQ68_08405"
     CDS             complement(3326..4735)
                     /locus_tag="PLQ68_08405"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HXK72714.1"
                     /translation="MNLLGLDFGTTSLKAAVFDLNGNCMFSHEETYELIHQGGFIEFD
                     AEKYWNILKRTIDLVTQQFSISALAIDTQCETMILADEQGNPLCNAIVWLDNRAELEA
                     YNIKKDFGNELIYNFTGQCDISGIWPACKLLWIKNNRPDVWKKTRKIFLLEDYLLYKL
                     TGRFVTQKTLQSSSLYMDIQKGCWWNKMLEYIGIQEDMLPSLLDSGVYVSDYKRISVA
                     TSAMDQAAAAIGAGIYKEGTISEMTGTAMAIFSPSNSMPKFNKDMLIPCHYNATTRYA
                     IMPWISTAGMALKWFRDNFVLDMSYSQLDMLAKDAPQGSDGLMFFPHLCGSTHPIFNP
                     EAKGSFYGIRLLHEKKHFVRAILESVSYMLRSSISSLNCDIETMHSIGGSSKSDLWCQ
                     IKADVTQKRIKTLKEKETACKGSAILSGLAINEYASLKSACEKAVVIDKEFNPGNSEY
                     EKLYNDYKSINEKIYERGK"
     gene            complement(4732..6231)
                     /locus_tag="PLQ68_08410"
     CDS             complement(4732..6231)
                     /locus_tag="PLQ68_08410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72715.1"
                     /translation="MNNTIKAGWYENSITPNKKVMLAGQFYERVSEYVETDIAVTAIA
                     VSSSQNHFVICSCDLLYIEDYLVDSVRKKITGRCEGLNPLNIVINATHTHTSIDYQRK
                     ENKDGSALNILKKYMPEDFSYIEKAKDDTIMSNNEAEEFLTDKISQAIIEAWNNKKTA
                     SFSNAFGRAPIGMCRRVVYEDSTGLMWGDTSRVDFESLEGGNDSGIELIYFFDEKRNL
                     TGIVANLACPSQILEHRSFISSDYWGKVKILLREKFGKDIYLLSLCSAAGDQCPRDLV
                     RWVAPVNPIDDPNIKRKDNINRIADPSMFEIEGSWKAAKRIANEIIDVYEDAKKTIKD
                     EAAIKHEYIKLRLPIRRASQEEYENATYIINQFVKNKKIKSFDFNDKAKMHVHAGTIA
                     RYIYQHKQSDYEIEMHVIKFSDLAIVTNPFELFLDYGNIIKARSMASQTFIIQLACGC
                     GSYLPTKKAEEHGHYSAYISSGITGHEGGKKLVETTLSIIEKLWGSENK"
     gene            complement(6242..7009)
                     /locus_tag="PLQ68_08415"
     CDS             complement(6242..7009)
                     /locus_tag="PLQ68_08415"
                     /inference="COORDINATES: protein motif:HMM:NF012669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HXK72716.1"
                     /translation="MEITVEERKEKILEMLNKRGKVRVNELSRIFNVSEVTIRLDLSD
                     LEESGLLSRVYGGAISSYKTYVNMSFKQRATANEFEKKAIALNVIQMIKENDTIMMNS
                     GTTTLFVYMAFPKSMHLTVVTNSVAIAQEASGNDNHKIILLGGSVNTDYQFTWGEDTL
                     NQLSAYHADKSILSVDGVSISAGLTTYYHQERVITQQMIAGSNLSIIAADYFKIGRAA
                     FSNITSADKADYIITNKTAPQEEIQNLIEIGIKVLTV"
     gene            complement(7146..7221)
                     /locus_tag="PLQ68_08420"
     tRNA            complement(7146..7221)
                     /locus_tag="PLQ68_08420"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7186..7188),aa:Lys,seq:ttt)
     gene            complement(7229..7303)
                     /locus_tag="PLQ68_08425"
     tRNA            complement(7229..7303)
                     /locus_tag="PLQ68_08425"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7269..7271),aa:Gln,seq:ttg)
     gene            complement(7320..7396)
                     /locus_tag="PLQ68_08430"
     tRNA            complement(7320..7396)
                     /locus_tag="PLQ68_08430"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7360..7362),aa:His,seq:gtg)
     gene            complement(7514..8938)
                     /locus_tag="PLQ68_08435"
     CDS             complement(7514..8938)
                     /locus_tag="PLQ68_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020458037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HXK72717.1"
                     /translation="MQKDTNPILEFSDNTNLLEQSAYKYQLQDRDTPNLYRYLYDYQS
                     VPKVPFNHRSVPMNMPKEIWITDTTFRDGQQSTSPFSVEQIVHLYKLMHKLGGPKGLI
                     RQSEFFVYTEKDRKAVEQCLSLGYKFPQITSWIRANEKDFDIVKGLGLEETGILVSCS
                     DYHIYNKMGLTRKQAMDKYLGVVKSVLDKGIRPRCHFEDITRADFYGFVVPFAYELTK
                     LSEQSKLPIKIRACDTMGFGVSYPGAALPRSVQGIIYGIMHYGGVPSELLEWHGHNDF
                     YKVVTNASTAWLYGASGVNCSLLGIGERTGNCPLEAMVIEYASLRGTNDGMKLQVITE
                     IAEYFEEAIGYEIPPRTPFVGRNFNVTRAGIHADGMLKDVEIYNIFDTEAILNRPPTV
                     AVDSHSGLAGVAHWLNSYFRIKDKAIMIKKTDPLVAKIKEAVDLEYANGRNTVMGDYE
                     LENILKEADYDEYERLEHLHIRKK"
     gene            complement(9200..10675)
                     /locus_tag="PLQ68_08440"
     CDS             complement(9200..10675)
                     /locus_tag="PLQ68_08440"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015486 -
                     glycoside-pentoside-hexuronide:cation symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide (GPH):cation
                     symporter"
                     /protein_id="HXK72718.1"
                     /translation="MDEVKTFNRNKWFYSTGGIGRDMAYQLFAAYLLTYVMFTKEITN
                     AQFAAISIIMIVCRIFDAINDPIMGGIIENTRMKLGKFKPWILIGALTNAVVMVLYFS
                     SPLQGWPFVIFFGFMYLLFDITFTMNDIGYWSMLPSLAKDQNQRATLTSMANLFASIG
                     AIIAFAAIPILTNGPNAIGGNSITGYLGVAVIIAIIFVACQVMTTIFVKEAPVSYRPK
                     NEKIGLKAMFQVVFRNDQLLWTSLVMLLYNLGSSLINVFGIIYVYLYFGYDGINITLF
                     VGAYAVGNLLINIVYPYMVKFMTRKQVATLGLIGVIVGYGFFFLVGVAIPMNYLLLCV
                     AGFILAFGQAMIYMVTTINLTNCIEYNEYKTGNRDEAIIFSIRPFMAKMASALQQGIV
                     AVAYLAIGITAITNGISEAERLNNMGLIDAVEKSEMIEKALALGTEATKMNFRIIIVV
                     VPIVLISIAYWIIMKKNKVNEKEYDQMIEDINAKKEINQAE"
BASE COUNT         3160 a         2230 c         1585 g         3844 t
ORIGIN      
        1 aaagattgtc tctctatctt tttattcatt tccctacagt aactttactc taactgaagc
       61 cattaagtcc ataatagaat acgaatattt atttactata aaaaaagcca taattaatat
      121 atggcttcat ttttattatc ttttctgtat cattccccta ttatctgtac ttctgctact
      181 ctttttcttt cacgaacctg atcccagtca ataaaatata tgaatccgaa atctcctaat
      241 gatagtttgc catcagttaa gacaatagtt tctgatcttc caaagaaaca tgatcttaag
      301 tgagcatctg tgttaaggct tccttttggt tcttcatctg gaaatgtaag tgcgaaatct
      361 atatgttttg gaccaggatg aagatactgg ccttcatacc tgcatgttgg cactattctt
      421 tccattatgt tgataaggtc ctgctgtaaa tgttctcttc caaaaaaagt cgtatcatgc
      481 gagcattcct gagtcattac agaacaagtt gtatgatgtg aatatactac acatattccg
      541 tttttaactc ctgattcttc cactatcttt ttcacctggt ccgtaatgtt gtgaaaatct
      601 ggcatgtggt ctttcgattg catttctatc tttcctgtgt aaactgtcat cttccatcct
      661 cctaatatta tttattatgt atttcatccc atgctcttcg aagactgtat atcatctctt
      721 ccaccatagc atatgggtct tttgctttaa ttatccctga tgtgcttcct gtagctaacg
      781 ctcccgcttt tataacgtta tacacatcat ttccatcctt aatacctgcg ccctgaagca
      841 ccattatttc aggattaatc tgtttaactg tttctataga ttcttttaca taagatatat
      901 ctgaagtctg tccggttcca ataagttctg tcggttctgc aacaatcaga ttaggtgaca
      961 ttttcgcgat ctgtttcatc tgctcaattg tatcagaaca cactatagta cccaaaccaa
     1021 cctcatctgc tctttctatt gttttttgta tttcttccat tgttaatggt ttttcagcat
     1081 gattcagcat tacacctaca gctccagctg cttttacagc ttctggaaga actgaaccta
     1141 accccctgcc aattggcaga taatccatat gctgagcaaa aaccagtatc cttgatgtat
     1201 tcaaagcaag taatgatata tcagtatact gaggtgtcac gattatatct acatcatact
     1261 tcattgccac cttatcaata acctttgcta aagaaagcat attttctccg tatagatatg
     1321 cttttgggcc tatctcaaaa aaaggtgctg agatttttaa tcctttatac atactttccc
     1381 cctatttttt atttaacagt tcacctactg caccacccgg atgaataatt ccgaactgct
     1441 cagaggtaaa gcctgtcttt actattagag ctgctaaaat cgaatcaaat aacgcgatag
     1501 tagcaacaaa gcttgaagtc gccattaatc ctaatggatc actttccttg tcaatattta
     1561 attttaagac tatatctgag cttcgagcaa gtatcgactc ttcattttca gtaacagcaa
     1621 taagggtagc tttctttttg ttacatactg atattatggg aagaagctct actgtctttc
     1681 cgcctcttga aaccattacc ataacatcat cactgtccaa agctcccagt ccgccatgaa
     1741 ccgcttcact tggtgacata aacatcgcat gtctttctat acagcataat gaatgagcaa
     1801 atttcttagc agctattcct gatgaaccgg aagccactgt cataatgctc ttgcaatttg
     1861 ctagtttttc tactgcttct aacaaagaat cttcgtttaa atacgatatt aatctttcta
     1921 tttccttttt ctctatatta agagatttac gtgctgcttc taatatttca cttctcatct
     1981 taccacctca atattaagta atttacataa atcaaccatc ttatcagcgt tatcttcata
     2041 tgcgattatg taatgctgac tcattacatt catagcgaat gtttccacat catctaaata
     2101 aatcattaat cccggaagct gaggagcagg ctgggtccat cccgcttctt cccatttatc
     2161 tgctgtttca gctctaccct ttacaatatg catacagtag ccttctttat tttgagaaag
     2221 cctgtaaaga atcacattac caggttttac tgaagacaca ttcaggatac cttcagacaa
     2281 ctgaccaaaa gctgcaggta taacaactgt atctccttgg gtaatttctt ttggtatata
     2341 gtcaggtact cctgccaaaa cacagtcttc cataaactca taaaattcaa ggtaagcacc
     2401 gcactgacca gttaaatatt taaccataag ttgtgtaacc attccatatg agtcattttc
     2461 cggaattgta cataacttat ctatgtcaga aagaagcata aatatgggtg caggaggata
     2521 tgataaaagt ttcttcattc catcaacatc ttttaacgaa acagcctcat atcctctttc
     2581 tttacagata tcggaaatag ccagataata tctagcagct ttttctttgg tgacactgtc
     2641 gcatgatttt aaaaatttcc atttttcgat ttcattatga ataacttcct gtatccttgt
     2701 tttagaaata cttacagctc tttgttccac ttccagtatc tcaaagcact ctatatctgt
     2761 tccaatatgt cttttgagtt ttactccgtc aaacatggtg ccgtaaaggt tcatatccct
     2821 atagcccatc attcctgctc tggatgattt tagtttgaac atggcatctg atgccttgca
     2881 gaaatcagca atttttttag agttcaatgg tttacccaca atctcatata caaatttaaa
     2941 cttgtatcct aagtcactca ttaccttcct tatagcgctt gttgccgcct ggtctgcagt
     3001 agtaacaata cgatcttttt ccatccatcc cgacaatccc cataaaacca taggtgtatc
     3061 tttaaatctc tcagttattg atattacagc atgtgaaggt atccatcctg ctatgcataa
     3121 tattacagtt tgaaatgatt ggctgacaag tgattcaatc gctttattta catctgacct
     3181 ttcataatca tcagttacag gatatatgct gataagctca ataccttctt ttaataatga
     3241 atcttctgcc tttctacatt tttctataat cagttccttt ggagtattca cttctccaaa
     3301 tccaataaat gctgctttag tcattttatt tacccctttc atatatcttt tcattaatac
     3361 tcttatagtc attatatagc ttctcatatt cgctatttcc tggattaaac tctttatcaa
     3421 taacaactgc tttttcacat gcgcttttta acgaagcata ctcattgatt gccaatcctg
     3481 atagaattgc actgccttta catgctgttt ctttttcttt caatgtcttt attctctttt
     3541 gtgttacgtc cgcttttatc tgacaccaca gatcagattt tgagcttcct cctatagaat
     3601 gcatggtttc aatatcacag tttaaagatg atatgctact tcttagcatg taagaaactg
     3661 attccaatat tgctctgaca aagtgttttt tctcgtgtaa tagccttatc ccataaaagg
     3721 aacctttggc ttcaggattg aatataggat gggtcgaacc gcaaagatga gggaaaaaca
     3781 taagcccatc agaaccttga ggagcatctt ttgcaagcat gtccagttgt gaataactca
     3841 tatccagaac aaaattatct ctaaaccact ttaacgccat ccctgcagtt gatatccatg
     3901 gcattatggc atatcttgtt gtcgcattat agtgacaggg aatcagcata tccttattaa
     3961 actttggcat gctgttcgaa gggctgaata ttgccatagc tgttcctgtc atctcgctta
     4021 tcgtaccttc tttatatatt cccgctccta tagcagccgc tgcctggtcc attgctgatg
     4081 tcgctacaga tattctcttg taatcagata cataaacccc gctatcaagt aaggatggaa
     4141 gcatgtcttc ttgtataccg atatattcta gcattttatt ccaccagcag cctttttgta
     4201 tatccatata caaactggat gactgtaatg tcttttgagt tacaaatctg cctgtaagct
     4261 tatataaaag ataatcctcc agaaggaata tctttcttgt ttttttccat acatcaggac
     4321 ggttattctt tatccacaat aatttacagg caggccatat acctgagata tcacattgtc
     4381 cggtaaagtt atatataagt tcattcccaa agtctttttt aatattataa gcttctaatt
     4441 ctgcacggtt atccagccat acaatagcat tacataaagg attaccttgc tcatcagcta
     4501 gtatcatagt ttcacactgg gtatcaatag caagagcaga aatgctaaat tgttgtgtaa
     4561 ctaaatcaat agttcttttc aatatattcc agtacttttc agcatcaaac tcaataaatc
     4621 caccctgatg tataagttca tatgtttctt catgcgaaaa catacagttg ccgtttaaat
     4681 cgaacactgc tgcttttaaa gaagttgttc caaaatccag tcccagaaga ttcatttgtt
     4741 ttcacttccc cataattttt ctattattga taaggtagtt tcgacaagct tctttccacc
     4801 ttcatgccct gttatcccgc ttgatatata tgcgctgtag tgaccgtgtt cttcagcttt
     4861 ttttgtagga agatatgaac cgcatccgca tgcaagctga attataaaag tctgagaagc
     4921 cattgatctt gcttttatta tgttaccgta atccagaaaa agttcaaaag gatttgttac
     4981 tattgctaaa tcagaaaatt ttataacatg catttcaatt tcataatcac tttgcttatg
     5041 ctgatatata tatctggcaa ttgtaccagc atgaacatgc atttttgctt tatcgttaaa
     5101 atcaaaagac tttattttct tattttttac aaactgattt attatgtatg ttgcattttc
     5161 atattcttct tgtgatgctc ttcttatagg aagacgcaat ttaatatatt catgtttaat
     5221 agctgcttca tctttaatcg ttttttttgc atcttcatat acatcaataa tttcattagc
     5281 tattcttttt gctgctttcc atgagccttc aatttcaaac attgatgggt cagcaattct
     5341 gtttatatta tcttttcttt ttatattggg gtcatctata ggattgacag gtgctaccca
     5401 tcttacaagg tctcttggac actggtcccc tgctgcagaa caaagactca gtaagtatat
     5461 atctttccca aacttctctc gtaaaagaat ttttaccttt ccccagtaat cagatgagat
     5521 gaagcttctg tgctccaata tctggcttgg gcatgcgaga tttgcaacaa ttccagtaag
     5581 atttctcttt tcgtcaaaaa agtatatcaa ttcaattcct gagtcgttac cgccttccaa
     5641 actttcaaaa tctactctac ttgtatctcc ccacattaaa ccggtagagt cctcatacac
     5701 tacccttctg cacataccta ttggagctct tccaaatgca ttggaaaaag atgcggtttt
     5761 tttattgttc catgcttcta taattgcttg tgaaatctta tctgttaaaa attcctctgc
     5821 ttcattgttt gacatgattg tatcgtcttt tgctttttca atatatgaga aatcttccgg
     5881 catatatttt ttaagtatat tgagcgcaga tccatcctta ttttcttttc tttgataatc
     5941 tattgaagta tgagtatgtg tagcatttat aactatattt aatggattca atccttcaca
     6001 tcttccagtt atcttttttc ttactgaatc aacaagataa tcctctatgt acaataaatc
     6061 acaagaacaa attacaaaat gattctgaga agatgatacg gcaattgctg ttacagctat
     6121 gtctgtttct acatattctg aaactctctc ataaaactgt cctgcaagca taactttctt
     6181 atttggagtt atactgtttt cataccagcc tgctttgatt gtgttattca ttacttcttc
     6241 ctcatactgt aagcactttt atgcctattt caattaagtt ctgtatttcc tcttgtggtg
     6301 cagttttatt ggttattata taatcagctt tatctgctga tgtaatgttt gaaaaagctg
     6361 ctcttcctat tttaaaataa tctgctgcta taatagaaag atttgagcct gctatcatct
     6421 gctgagtgat tactctctcc tgatgataat atgttgtaag tccagcacta atgctaaccc
     6481 catcaaccga gagaatggat ttgtctgcat gataagctga taactgattt aatgtatcct
     6541 caccccatgt gaactgataa tcagtattaa ctgaaccgcc tagcaagata atcttgtgat
     6601 tatcattacc tgaagcttcc tgtgctatcg ctacagaatt tgtgactact gtcaaatgca
     6661 tgcttttagg aaaagccata tatacaaaaa gtgtagtagt tccagagttc atcataatgg
     6721 tatcattttc cttaatcatc tgaattacat ttaatgcaat agcctttttt tcaaattcat
     6781 ttgcagttgc gcgttgtttg aagctcatat taacataagt tttgtatgat gaaatagctc
     6841 ctccgtaaac tctggataga aggccgcttt cttctaaatc agaaaggtcc agtcttatag
     6901 taacttcaga tacattaaat atacggctaa gctcatttac tctgacttta cctcttttat
     6961 tcagcatttc aagtattttt tcttttcttt cttcaactgt tatttccatt gattgccagg
     7021 tttcattatg ttttatttgt gtttcgtttc actttcgatt atatgatata accatattta
     7081 ttctaatgca acaattttat aaaataattt tatatagtaa aaaacttccc tattaggaag
     7141 ttttttggtg agacatcgga ggctcgaact ccggacacct tgattaaaag tcaagtgctc
     7201 taccacctga gctaatgtcc cataagaatg gctgggatgg caggatttga acctacgggt
     7261 gacggagtca aagtccgttg ccttaccact tggctacatc ccatcatctc aaatcaaaat
     7321 ggggtgaata aagggattcg aacccttggc ctctagggcc acaacctagc gctctaacca
     7381 actgagctat acccaccatg gtcgaacaaa gtcttttatc attctaatgg ctggggtact
     7441 gtttgtcaac acattttatc acgaaattca aaatttgtga aattgaaaaa aaggaggttt
     7501 ttacctcctc aaatcatttc ttccttatat gcagatgttc aagtctctca tattcgtcgt
     7561 aatctgcctc ttttaagata ttttctaact cataatcacc cataacagta tttctaccgt
     7621 ttgcatattc taaatctact gcttctttta tctttgcgac aaggggatct gtcttcttta
     7681 tcataatcgc tttatccttt atacggaagt atgagttaag ccagtgagct actccggcaa
     7741 gtccgctatg tgaatcaact gcaactgtag gtggacgatt caatattgct tctgtgtcaa
     7801 aaatattata tatctctaca tccttaagca ttccatcagc atgaatacca gctcttgtta
     7861 cattgaagtt tcttcctaca aaaggagtcc ttggaggtat ttcatatcct attgcttctt
     7921 caaagtactc agcaatctcc gttattacct gtaatttcat tccatcattc gtacctctga
     7981 gtgaagcata ttcgataacc atagcttcaa gaggacaatt gcctgttctt tctcctattc
     8041 caagcaagga acagttaact cctgatgcgc cgtaaagcca tgcggttgat gcatttgtta
     8101 caactttata gaaatcgttg tgaccatgcc actctaaaag ctcacttggc actccgccat
     8161 aatgcattat gccatatatt ataccctgaa cacttcttgg aagagcagca cctggataac
     8221 tgacaccaaa tcccatagta tcgcaagctc tgattttaat cggcaatttt gactgttctg
     8281 atagttttgt aagttcataa gcaaaaggga caacaaaacc gtaaaaatct gcacgggtaa
     8341 tgtcttcaaa atgacagcga ggtcttattc ctttatccag aacacttttt actacaccaa
     8401 gatacttatc catagcctgc tttctggtaa gtcccatctt attgtaaata tggtaatctg
     8461 aacatgatac cagaatacct gtttcttcca atccaagtcc ttttacaata tcaaaatcct
     8521 tctcatttgc ccttatccag ctagtaatct gtggaaactt gtatcccaaa cttagacact
     8581 gttcaacagc cttacggtct ttttctgtgt atacaaaaaa ctcgctctgt cttattaaac
     8641 cttttggtcc accgagttta tgcatcagtt tatataaatg tactatctgt tcgacactga
     8701 atggtgatgt tgactgctga ccgtcacgaa aagtcgtatc ggttatccat atttctttag
     8761 gcatattcat tggaacactt ctgtgattaa aaggcacctt gggaactgat tgataatcat
     8821 atagatatct atataaattc ggagtatctc tgtcttgaag ctgatacttg tatgccgact
     8881 gctctaaaag gttcgtattg tctgaaaatt ccagtattgg gtttgtatct ttttgcataa
     8941 tttatttcta atcctttcat ttttagaaca catttcataa attattccgt attatacaat
     9001 tgaatatgaa ttattgcaat acatattttt cattccatga ataaaatttt atttacatgc
     9061 tttctgtttt aattttaatt caaaatgagt gaatatatgt aactatcatt attttaaata
     9121 aatcaaacaa tgtttattct tacataaaat cagatatggg ttatccatag ctgatttatt
     9181 tatctaaact cttattatat tattcagcct gatttatttc tttttttgca ttaatgtctt
     9241 caatcatctg gtcatactct ttttcattca ccttattctt cttcataata atccagtatg
     9301 caattgaaat gagcactatc ggtactacaa ctataataat cctaaaattc attttagttg
     9361 cttcagttcc taaagctaat gctttttcta tcatctcact cttttcaaca gcatcgatta
     9421 aacccatatt atttaatctt tcagcttcag atatcccatt tgtaatagct gttattccaa
     9481 ttgcaagata cgcgacagca acaattccct gctgaagagc agaagccatt ttggccataa
     9541 atggtcttat tgagaatatt attgcttcat ctctgttacc tgtcttgtat tcgttatatt
     9601 caatacagtt cgttagattt attgtagtca ccatataaat cattgcctgt ccaaatgcta
     9661 atatgaagcc tgctacgcat aacaaaagat aattcatagg tatcgctaca ccgaccaaga
     9721 aaaagaaccc gtaccctact atgacaccta tcagtcctaa agtcgcaacc tgttttctgg
     9781 tcataaattt aaccatatac gggtatacaa tgtttataag aagatttcct actgcgtatg
     9841 ctccaacaaa caaggttata tttattccgt catatccaaa atacagataa acatatatta
     9901 ttccgaatac attgattaaa gatgatccaa gattatacaa aagcataacc agtgatgtcc
     9961 ataaaagctg atcgttacgg aatacaacct gaaacatagc cttaagacca atcttctcat
    10021 tcttcggtct ataagaaaca ggagcttctt ttacgaatat tgtagtcatt acctgacagg
    10081 caacgaagat aattgcaatt attactgcaa ctcctaaata acctgtaatg gaatttcctc
    10141 caattgcatt agggccattt gttaatatgg gtattgctgc aaatgcaatt atagctccta
    10201 ttgatgcaaa cagattagcc atagaagtca atgtagctct ctggttctgg tctttagcta
    10261 atgaaggaag catggaccag tatccaatat cattcatagt aaatgtaata tcaaacaaca
    10321 gatacatgaa accaaagaat attacaaaag gccatccctg cagaggagat gagaaatata
    10381 gaaccattac aacagcattt gttaatgcac ctatcaatat ccatggtttg aatttaccca
    10441 gtttcatacg cgtattttca ataattccac ccattatagg atcattgatt gcgtcaaata
    10501 tacggcatac tatcattatt attgatattg cggcaaactg agcattggtt atctcttttg
    10561 tgaacataac gtatgtaagc agatatgcag caaaaagctg gtatgccata tcacgcccta
    10621 taccacctgt agagtagaac catttgttgc gattaaatgt ttttacttca tccataccct
    10681 tctcctttta ttaaattgca tataaaacaa tattattagt gtaattatat accaaaatat
    10741 tcaagtttca aacaaaatga atattttgat agtgtaaact tgtttgggga ttgataaaat
    10801 aaaagagaaa tcccattta
//
LOCUS       DAOSXM010000068        10638 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2111
            NODE_3933_length_10638_cov_11.442219, whole genome shotgun
            sequence.
ACCESSION   DAOSXM010000068 DAOSXM010000000
VERSION     DAOSXM010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864178
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 10638)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10638)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:24:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,261
            CDSs (total)                      :: 2,229
            Genes (coding)                    :: 2,218
            CDSs (with protein)               :: 2,218
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 3 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10638
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3933_length_10638_cov_11.442219"
                     /isolate="AMR_MDS_2111"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(46..1002)
                     /locus_tag="PLQ68_08445"
     CDS             complement(46..1002)
                     /locus_tag="PLQ68_08445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72719.1"
                     /translation="MDNLSKSRKDEFSFKCADFIPFKDKEVIERVRKIHGQALTKHSN
                     PDFKINIIPDALIENIFVTDMFYEIKKASEEGRKVVFIIPNPNPSYLKLAWMINRFKL
                     DVKHVYTFNMDEWADDMGNIAPESWNASFMNSTKRFFYSQIDPKLRMDEKQIQGPTNE
                     NIEDYSDMILQAGGADVCYSGPGWTGHLAFIEPDVPEFAGSLDEFLKMGARVTSLNPL
                     TVAQNSLHGCFGASGDLAAVPPKAATIGPKDVLAAKRRFEIHSLTTAGTFVTWQRMIS
                     RLVLHGPVTPKVPSSILQLAPTDVYVSETLAAPIEPDWIFQY"
     gene            complement(995..2359)
                     /locus_tag="PLQ68_08450"
     CDS             complement(995..2359)
                     /locus_tag="PLQ68_08450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72720.1"
                     /translation="MALFKAGVRQTDLSPDKGVDLGGYPYFERANVGIHDPLYGTCLY
                     LVNDKCESVLLMTSDLCYMTKRQCDMVKEQVYAKTGILKESIIMTFSHSHSAPWMSAM
                     FRSLPGQPEYESKVDEDYLKVVINKWSDCAKEAFDNLFDASIGFTKTRCGKAEGIGGN
                     RRDPGNGLSDEDVPVMVIKDKDNVIRAIHTKYALHPTILHGENKYVSADYPGYIRKHI
                     NGKYPDAVFMFSQGTSGNQSPRYFRKDQSFSEAERFGKTIALSILEIIDNVIYTDSLK
                     ILHSTRVLELKIRNYSDIESAKAKVEYYKKKKEEKIKNNGSYTEIQTADMWLLGAECE
                     YNYSLMYQKGELESYYKNDIPFTAHGLVLNEHAYTFTQGEMFVEFGLRIKELSPFANT
                     TVITVSNGMTPGYCVTKEAYEEGGYEAWNSLLDTKCGDIVVNSLLDMLTGMHMRYKEE
                     RRIG"
     gene            complement(2368..3816)
                     /locus_tag="PLQ68_08455"
     CDS             complement(2368..3816)
                     /locus_tag="PLQ68_08455"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HXK72721.1"
                     /translation="MSKYLIGIDLGTTVCKCSIYDDSMKLVSQAGVEYPLIYKSKGHI
                     EQDANLWWELSKEVVVKALSVIKDKENVKAISVSSQSMAFVPLDEYGNTISNAISWLD
                     ERGHSELERIKEEFGESNIYNKTGKWLYGAYVLPKLLWLQKNEPDFFLASKIAFPMDF
                     LVYRLCGRLITDRTIASGTMMYNIVKNEWDQEILDKFGIDNSILPEIKISATVAGYIK
                     EDVAKDLGLNFDVEIVVGGQDQKCASIAAGINESVCTVSLGTAAAIEVLYDKPVMDPD
                     MKIPVFSYLTDNHWVSETAIACAGAAVKWYQKNFASSYSYDQLDEMAITEYAKQNGIF
                     FYPYLNSNDAFSGAFYGLGLDSDMRKTFIAVREGIAFEISMLIDEMKAVPGAKAFEGI
                     VLFGGGSYSRLWCQLIADVCNTPVIVKDTSEMAAKGACIIAAVGAGIYENPFNALNQD
                     TDTLIYKPVAGNVNIYMEKKIAYQRLKKKMFS"
     gene            complement(3821..4267)
                     /locus_tag="PLQ68_08460"
     CDS             complement(3821..4267)
                     /locus_tag="PLQ68_08460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72722.1"
                     /translation="MISCTEFIPAYSQVFKYLHEKEGYDGVVRFWEHISDEYVEPRLG
                     KLIKQKGIAGCYEYWSKSLNEENADFIMTLDEEKGEFEIVMNKCPSKGMLLTLDESNV
                     YPHYCDHCDLLYRKVVEKYGMSYYFEPDKDNLACCRLKVTKKENIR"
     gene            complement(4272..4724)
                     /locus_tag="PLQ68_08465"
     CDS             complement(4272..4724)
                     /locus_tag="PLQ68_08465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72723.1"
                     /translation="MTKLVMERKMGDNTYLHKDFHVSMDIALAYIDRRYKKEGVTEYL
                     NRFTDSYHKDLINKISQEGLSPFKTYLENIFKAEECSDALSIIESEGTLHVKISKGFY
                     VYTISGYSQLALEKVYGTIIQKAGYRFELLFFDDMTGACEFKVKRIGR"
     gene            complement(4726..5520)
                     /locus_tag="PLQ68_08470"
     CDS             complement(4726..5520)
                     /locus_tag="PLQ68_08470"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXK72724.1"
                     /translation="MNILQSLSLENKVVLITGGRQLYGKSSSIALAQAKATLYIATHS
                     IEKALPFIEELNKMGGKAYAVQFDQEDPVSIKKMIDHVTDQQGRIDVFINASRVKPEN
                     FGWNQKMSDLEYSIRCNGAAFLYMNRIVGQQMIKQKSGSMILFGSMMGLVGVESHNYD
                     GADEMKKGAYSHDYAIIKSGVNGWTRHAASYYGRYGIRVNAICPGGLETPDMNPSFVK
                     NYSLHTQLGRLADNEDIKGIVVFLASDTSKYITGAILPVDGGYVSI"
     gene            complement(5517..6269)
                     /locus_tag="PLQ68_08475"
     CDS             complement(5517..6269)
                     /locus_tag="PLQ68_08475"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXK72725.1"
                     /translation="MENLFSVKGKKILICGNDAKYLHEIAKGLDKAMALVYVCGPHYI
                     SGLNGFEFFELQCDTEENLLSSLNLIKNRIGNIDCFIWADTQKPHPGWDHEFEVIDET
                     LKRHMLGLVLCVKHIGGFMAEKKNGSIIVIADYSALVGLDVNNYQDNDELFNTDFSLE
                     YGFIQGGYINYTRQAAGYLGQFNVRCNVVACAPLKDTYPSEFEQKFIKHSHIKKMAEK
                     EDILSAVIYLSSDASKYVTGTVIPVDGGYTAK"
     gene            complement(6272..7237)
                     /locus_tag="PLQ68_08480"
     CDS             complement(6272..7237)
                     /locus_tag="PLQ68_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010268985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HXK72726.1"
                     /translation="MPDLWPLKDKQLKIGILGMTEGNGHPYSWSAIFNGYNKECMDKS
                     PYPGIAEYLNKQPLSNFGIEGANITHICCDNYEDALSVAKCSNVKNIVRTALDMIGHV
                     DAVICATDIPSEHVRRCKPFIEAGIPVFIDKPLADNEEDLKTFVRWRDAGHHFLSSSS
                     MRYKKSLEPYHKNHHELGELMYICQPMCKKWETYGIHALESVYPFLGPGFLTVQNTGT
                     YESNMVHITHKSGCDVHIPLKKEMYGAFSVTLLVGRYGNISLSDNDHYYSFKKQLEMF
                     VRYLRTGIEPFDFKETIELTKMVIAGILSLKSEGGVICLDDIKER"
     gene            7398..8177
                     /locus_tag="PLQ68_08485"
     CDS             7398..8177
                     /locus_tag="PLQ68_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010268978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HXK72727.1"
                     /translation="MNFRPSRVLERIKNGYVATCTKLNTYDSRVASIAALAGFDCIWV
                     DLEHIPNSISDIEKLILTSKAYETDIITRVKRGSYSDLIHPLEADSTGIMVPHVMSFD
                     DAKEIQYYTKFHPVGRRPLDGGNADGMYGMKDTKEYMDFMNNNRFIALQIEDPEPLDE
                     LEQICKLPGADMIFFGPGDFSQGIGTPCVWDNPKISATRKLVAKMARENNKIAGTVGN
                     LNNFDMLINEGYNFISIGADVIGLGEYFMNISDKVKEKKAI"
     gene            8174..>8490
                     /locus_tag="PLQ68_08490"
     CDS             8174..>8490
                     /locus_tag="PLQ68_08490"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HXK72728.1"
                     /translation="MKTISKMTLGTVQLGMEYGINNTTGKPDMKAAHEILRTALQNGI
                     TSLDTANAYGDSENVIGSYLKQSNDNPFITTKFFTTSPPGTKSEDIEKEIYGYVENSL
                     QRL"
     assembly_gap    8491..8590
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            8905..10638
                     /locus_tag="PLQ68_08495"
     CDS             8905..10638
                     /locus_tag="PLQ68_08495"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HXK72729.1"
                     /translation="MIRRFRSIFLIAYLIGVLALLTVTFTSARAENISMLVDQYTESF
                     RTTELIFTNYFQEIQNDLLTLSENEIVRTRDDEHFTSFLFADENSFEYNYSTEELRII
                     QIFKSYLDNHPNANSVYMGRENGSFVRAVPRTSPTRYDPRTRPWYIAAMKTPGILQMT
                     VAYPSVTNDDINIAFVITLNDNNNMPYGVIGIDVTLETLSEVLKNQQLLFEGFMEVVD
                     DQNMIVMTPKDENLYTLNKQETNYRQLEQTDTVTIERNKLFYKVTYTKELWDSTLIAY
                     APTKVVERSLVSNLISKILWSFIILLIIEASNYLFIKRYIDKPIKELITSLKESQKTD
                     IPKKISLKAKGEFKQFEKQYNELVDSLKQNENDLKRIRNIIITSLSSLTSMRDFETGL
                     HLYRASKYVNLLAEAYNEKNPEEPIPSQIIDIMTESAPLHDIGKIAISDEILKKKGPL
                     TTEEFEIMKKHTLYGKQMFEKIMTGIENRDFIKTAHNMIYYHHERWNGKGYPENLSNN
                     NIPLEARIMSIADAYDAMTSTRVYSPAISHEEAKAELIRCKGTQFDPNLIDVFLSIED
                     SIKAVAEAYKE"
BASE COUNT         3281 a         2142 c         1715 g         3400 t
ORIGIN      
        1 atttttaata cagagcatat ccactcctta atactggaat tccagttagt actggaatat
       61 ccaatcaggc tctattggtg ctgccaaagt ctcgcttaca taaacatcag taggcgcaag
      121 ctgcagtatt gatgaaggca ctttaggtgt tacaggacca tgaagaacca gtcttgatat
      181 catcctctgc caggtaacga aagtaccagc tgttgtaagg ctgtgtatct caaagcgtct
      241 ttttgctgca agcacgtctt taggtcctat tgttgcggct tttggaggta cagccgccaa
      301 atcccctgat gctccaaaac atccatgtaa cgagttttga gccaccgtta atgggttaag
      361 agatgttact cttgccccca tctttaaaaa ctcatccaat gagcctgcaa attccggcac
      421 atcaggctct ataaatgcaa gatgcccggt ccagcccgga ccgctgtaac atacatcagc
      481 tccacctgct tgtaaaatca tatcgctgta atcctctata ttctcattcg taggcccttg
      541 tatctgtttt tcatccatgc gaagcttggg gtctatctgg ctatagaaga atctttttgt
      601 gctgttcata aaacttgcat tccacgattc tggagcaata ttacccatat catcagccca
      661 ttcatccata ttgaaggtat aaacatgttt cacatccagc ttgaaacggt ttatcatcca
      721 tgctagttta aggtatgaag ggttgggatt tgggataatg aatacaacct ttcttccttc
      781 ttctgatgcc ttctttatct cataaaacat atctgtaaca aaaatattct caattaaagc
      841 atctggaatt atgtttatct taaagtccgg attgctgtgt tttgttaaag cctgcccgtg
      901 tatcttcctt actctttcaa taacttcctt atccttaaat ggtatgaaat ctgcacattt
      961 aaatgaaaac tcatcttttc ttgatttact taaattatcc aattcttctc tcctccttgt
     1021 atctcatatg cataccggta agcatatcca gtaagctatt tacgactata tctccacatt
     1081 tcgtatctaa aagcgaattc catgcttcat atccgccttc ttcataagct tcttttgtaa
     1141 cacagtaacc aggtgtcatg ccattgctga cggtaataac agtagtgttt gcaaatgggg
     1201 ataattcttt tattctcaaa ccgaactcca caaacatctc accttgtgta aatgtatatg
     1261 catgttcatt aagcacaagg ccatgagcag taaaaggaat atcattttta taatatgact
     1321 ccagttctcc tttttgatac attaaggaat aattgtattc acattccgct cccaaaagcc
     1381 acatgtctgc tgtctgtatt tcggtatatg agccattgtt ttttatcttt tcttcttttt
     1441 tctttttata gtattctact tttgccttag ctgactcaat atctgaataa tttcttatct
     1501 taagttcaag tactctcgta gaatgaagta tctttaatga atctgtatat atgacattat
     1561 ctattatttc caaaatactt agtgcaatgg ttttaccaaa tctttctgct tcagaaaaac
     1621 tctgatcttt tctgaaatat cttgggctct ggtttcctga cgttccctga ctgaacataa
     1681 atactgcatc aggatatttc ccatttatat gttttcgtat gtacccaggg taatcagcag
     1741 aaacatattt attctccccg tgaagtattg taggatgaag agcatacttt gtatgtatcg
     1801 cacgtataac attatcttta tctttaatca ccattaccgg gacatcttca tctgataacc
     1861 cgtttcccgg gtctcttctg ttcccgccta ttccttcagc tttgccacat ctggttttag
     1921 taaaacctat acttgcatca aataaattat caaaagcttc ttttgcacag tctgaccatt
     1981 tattgataac taccttcaga taatcttcat caacttttga ttcatactca ggctgtccgg
     2041 gtaatgatcg aaacatcgca ctcatccatg gagcgctatg cgaatgtgaa aaagtcatta
     2101 ttatactttc ttttaaaatg ccggtttttg cataaacctg ctcctttacc atatcacatt
     2161 gccttttagt catatagcac aagtcacttg tcatcagtaa tactgattcg cacttgtcat
     2221 taacaagata aagacatgtt ccatatagcg ggtcatgtat tcctacattc gctctttcaa
     2281 aatacgggta gcctcctaaa tccacacctt tatccggcga cagatctgtc tgccttactc
     2341 ctgctttaaa tagtgccatc aatcctccta gctaaacatt ttcttcttta atctttggta
     2401 agctattttt ttctccatgt atatattaac attaccagca actggtttgt atattaaagt
     2461 atctgtatcc tgattaagtg cattaaatgg gttttcataa atacctgctc ctactgctgc
     2521 tattatacaa gctccctttg ctgccatttc tgatgtgtct ttaacaataa caggcgtatt
     2581 acatacatca gctatcagct ggcaccatag tctgctgtat gatcctcctc caaaaagcac
     2641 tattccttca aacgcttttg cgcctggtac tgctttcatt tcatctatta acattgatat
     2701 ttcaaatgct atgccttctc ttacagctat aaatgttttt ctcatatcgc tgtcaagacc
     2761 aagaccataa aaagcaccag aaaaggcatc attagaattc aagtaagggt aaaagaaaat
     2821 tccattttgc tttgcgtatt ctgttatagc catttcatct aactggtcat aactgtatga
     2881 tgaagcaaaa ttcttctgat accactttac tgctgcacct gcacatgcta ttgctgtttc
     2941 acttacccag tgattatctg tgagatagga aaataccggt attttcatgt ctgggtccat
     3001 tacgggttta tcataaagta cctctatagc tgctgcagta cctaaagaga ctgtacacac
     3061 cgactcgttt attcccgctg ctatagatgc gcatttctgg tcctgccctc cgacaactat
     3121 ttcaacatca aagttcaatc ccaaatcctt tgctacatcc tctttgatat atcctgcaac
     3181 agttgctgaa atttttattt caggcagtat gctattatct atcccgaatt tatccagtat
     3241 ttcctggtcc cattcatttt ttacaatgtt atacatcata gttcctgatg ctatagtcct
     3301 gtctgttata agtctgccgc aaagcctata aacaagaaag tccataggaa aagctatttt
     3361 tgatgctaaa aagaaatcag gctcattttt ctgaagccat aacaactttg gcaacacata
     3421 tgctccatac agccacttgc ctgttttgtt atagatattg ctttctccaa attcttcttt
     3481 tatacgttct aactcggaat gccctctttc gtccagccag ctaatggcat ttgaaattgt
     3541 attaccatac tcatccaaag gcacgaaagc catactctga gaactaacgg atatggcttt
     3601 tacgttttct ttatccttta taactgacaa ggcctttaca acaacttctt tagataactc
     3661 ccaccaaaga tttgcatcct gctctatatg tccttttgac ttatatatca aaggatactc
     3721 cacccctgct tgtgaaacaa gtttcataga atcatcatat atggagcatt tgcatacagt
     3781 tgtgcccagg tctattccaa ttaaatattt actcataact ttacctgatg ttttctttct
     3841 ttgttacttt cagcctgcag caggctaagt tatctttgtc tggttcgaaa tagtatgaca
     3901 taccatattt ttcaacgact ttcctgtata gcagatcaca gtggtcacag taatgtggat
     3961 aaacattaga ttcatccaaa gtcagaagca tcccttttga cgggcatttg ttcataacta
     4021 tttcaaattc ccctttttct tcatcaaggg tcataataaa gtcagcattc tcctcattaa
     4081 ggctttttga ccagtattca tagcatcctg caataccctt ttgttttata agtttgccta
     4141 atcttggttc tacatactca tcagatatgt gttcccagaa tctgactact ccgtcatatc
     4201 cttctttttc atgaaggtat ttaaaaacct gagaatatgc tggaataaat tctgtacatg
     4261 aaatcatatt ctcacctgcc tatccttttt actttaaatt cacatgctcc ggtcatatca
     4321 tcaaaaaaca acagctcaaa tctgtaaccg gctttttgta taattgtgcc ataaaccttt
     4381 tctagcgcta actgactgta accgcttatg gtatatacat aaaacccttt gcttatcttt
     4441 acatgtaaag taccttcact ttcaattatt gaaagggcat ctgaacattc ttcagctttg
     4501 aagatgttct ctaaatatgt cttaaatgga gataagcctt cttgtgatat tttattaata
     4561 aggtctttat gataggaatc tgtaaatctg tttaaatatt ctgtgacgcc ttctttttta
     4621 taccttctat caatatatgc aagagcaata tccatgctta catggaaatc cttatgaagg
     4681 tatgtattat cacccatttt tcgttccata accagttttg tcatatcaga tgcttacata
     4741 acctccatca accggcagta ttgcacctgt aatatatttt gatgtatcag atgctaagaa
     4801 caccacaatt cccttaatat cctcattatc tgctaacctg ccaagctggg tatgcaaaga
     4861 atagttctta acaaatgacg gattcatatc tggtgtttcc agtcctcccg ggcatatcgc
     4921 attcaccctt ataccatatc tgccataata gcttgctgca tgtcttgtcc atccattcac
     4981 tccggatttt ataattgcgt aatcatgcga atatgcgcct tttttcattt cgtcagctcc
     5041 atcatagtta tggctttcta ctccgacaag acccatcata gaaccaaaca gaatcatgga
     5101 gccgcttttc tgttttatca tctgttgtcc tacaatccgg ttcatataca aaaaggctgc
     5161 cccgttgcat cggattgagt attctaagtc agacatcttc tgattccatc cgaagttctc
     5221 aggtttaact ctgcttgcat ttataaatac atcaattcga ccttgctggt ctgttacgtg
     5281 atcaatcatt ttttttatgg ataccggatc ttcctggtca aattgcactg cgtaagcttt
     5341 tccacccatt ttgtttagtt cttcgataaa aggaagagct ttttctattg aatgtgtcgc
     5401 tatatacaaa gtagctttag cctgagctaa agctattgag ctggatttcc cgtaaagctg
     5461 tcttccgccc gttataagaa ctaccttgtt ttccagagaa agactttgta agatattcat
     5521 ttcgcagtat aacctccatc tactggtata actgttcctg ttacatattt agaagcatcg
     5581 gaagaaaggt atattactgc tgataaaata tcttcctttt ctgccatttt cttaatatgt
     5641 gaatgtttga taaatttctg ttcaaattca gaaggatatg tgtcttttaa tggtgcacag
     5701 gctacaacat tgcaccttac attaaactgc cctaaatatc ctgctgcctg tcttgtataa
     5761 ttaatatatc cgccttgtat gaaaccatac tcaagggaaa aatccgtatt aaaaagctca
     5821 tcattatctt gatagttgtt aacatcaagc cctacaagtg cagaataatc tgcaataact
     5881 ataatcgagc cattcttctt ttctgccata aaaccgccta tatgttttac gcataaaacc
     5941 aacccgagca tatgcctttt caatgtttca tctattactt caaattcatg atcccatcct
     6001 gggtgaggtt tttgcgtatc ggcccatatg aagcagtcta tgttccctat acgatttttg
     6061 attaaattca gtgaagataa taagttttcc tcagtatcac attgcagttc gaaaaattca
     6121 aatccgttaa ggccggatat atagtgagga ccacagacat ataccaaagc cattgcttta
     6181 tctaatcctt ttgctatctc gtggagatat tttgcgtcat ttccacatat gagaatcttc
     6241 ttccccttta cgctgaacag gttttccatg gtcatctctc ttttatgtca tccagacaga
     6301 tgacccctcc ttcagatttt aaacttaata tgcctgctat aaccattttt gtaagttcaa
     6361 tggtttcttt aaaatcaaat ggttcaatac ctgttcgtaa atagcgcaca aacatctcta
     6421 actgtttttt aaaggaataa taatggtcat tgtcagacaa tgaaatattc ccatatcttc
     6481 caaccagtaa agtaacggaa aatgccccat acatctcttt tttaagaggt atatgtacat
     6541 cacagcctga tttatgagta atatgtacca tattgctttc atatgttccg gtattttgca
     6601 ctgtcagaaa gcccggtcct aagaaaggat aaactgactc taatgcatgt atgccataag
     6661 tttcccattt tttgcacata ggctggcata tatacataag ctctcccagc tcatgatggt
     6721 ttttgtgata tggttccaaa cttttcttgt atctcattga tgaagaggat aaaaaatgat
     6781 gacccgcatc tctccacctg acaaatgtct ttaaatcttc ttcgttatct gctaaaggct
     6841 tgtctatgaa aacaggtatg cctgcttcaa taaagggttt gcatcttctt acatgttctg
     6901 aaggtatgtc tgtagcacat atgactgcat caacatgtcc tatcatatcc aaagcagttc
     6961 taacaatatt tttaacatta gagcatttag ccactgataa agcatcttca tagttatcac
     7021 agcatatgtg agtaatattt gcaccttcaa tgccgaaatt acttaaaggc tgcttattca
     7081 gatactcagc aatacctgga taagggctct tatccatgca ttctttgtta tatccattaa
     7141 aaatcgcact ccaagaatat gggtgaccat taccttcagt catacccaaa ataccaattt
     7201 ttaactgttt gtcctttaga ggccacaaat ctggcattag gacttccccc caatgttgaa
     7261 ttgatatata acagtgcttg ctatatatat tattcgtttt aaagaaaaat ttcaatgcaa
     7321 ttatattatt ttattaaaaa tatttaatcc tgttatataa tttaacaaag gttaaaaata
     7381 tatcagtgga gataattatg aatttcagac caagcagggt tctcgaacga ataaaaaacg
     7441 gttacgtggc tacatgtacc aaacttaata catacgattc cagggtagca agtattgctg
     7501 cattggcagg attcgattgt atctgggtag atcttgagca tatacctaat tcaataagtg
     7561 atattgaaaa acttatctta acatcaaaag cctatgagac agatataatc acaagagtga
     7621 aaagaggtag ttattctgat cttatccatc ctttagaagc agacagcact ggtattatgg
     7681 tgcctcatgt aatgagtttt gatgatgcaa aagaaataca gtactatacg aaatttcatc
     7741 cagtcggcag aagaccatta gatggaggca atgctgacgg gatgtacggc atgaaggata
     7801 caaaagagta tatggatttt atgaataata acagattcat tgccttgcaa attgaagacc
     7861 ctgagccttt agacgagttg gagcagatat gcaagcttcc cggagctgac atgatattct
     7921 tcggaccagg cgatttttca caaggcattg gaactccctg cgtttgggat aatcccaaaa
     7981 tatcagccac aagaaaactg gttgcaaaaa tggcaagaga aaacaataag atagcaggaa
     8041 cagtgggaaa ccttaataat tttgacatgc ttatcaatga ggggtacaac ttcataagca
     8101 tcggagctga tgttatcgga cttggagagt atttcatgaa tatatcagat aaggtaaagg
     8161 aaaagaaagc aatatgaaaa cgatatctaa aatgacatta ggaaccgtgc agctgggaat
     8221 ggagtacgga atcaataaca caactggaaa acctgacatg aaagctgctc atgaaatatt
     8281 aagaacggct ttgcaaaacg gtataacttc cttggatacg gcaaatgcct atggtgattc
     8341 tgaaaacgtt atcggaagct atttgaaaca atcaaatgat aaccccttca taactacaaa
     8401 gttctttacg acatcacctc ctggtacaaa aagtgaagat atagaaaaag agatatatgg
     8461 atatgttgag aactcattac agaggcttaa nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8581 nnnnnnnnnn acaaccaaga ataataggaa actcagattt gcataagatt ctgcaattga
     8641 gtgtgaaggt ttttctgttt atgcaaatgg cggcctgagt attgataaaa ttatgacgac
     8701 ttcaggtgaa aaaaatattt gagataacaa aataaaaata caaaagcact gtttgttccc
     8761 ttcgtttgaa tgaggatgtc tttattattg caaatgagtt tgctgtaaaa tagcaatatt
     8821 gtgttataaa agcaaagaaa tattgatatt aaatatatca tatgatatat ttatatagca
     8881 taaatcccta tattgtaggt gcctatgata agaagattca gatcaatttt tctaatagca
     8941 tatctgatag gcgttcttgc tcttttgaca gttacgttca cttcagccag ggctgagaac
     9001 atatcaatgc ttgtagatca atatactgaa agctttcgaa ctacagagct tatcttcact
     9061 aattactttc aggaaataca gaatgacctt ttgactttat cagagaacga gattgtaagg
     9121 acgcgagatg atgagcattt cacgtccttt ctttttgctg atgaaaattc ctttgaatac
     9181 aactactcaa ctgaagaatt aagaataata caaatattta agagttatct tgacaatcat
     9241 ccaaatgcta attcggtata tatgggaaga gaaaacggtt cttttgtaag agcagtacca
     9301 agaacctctc ctacaagata tgacccgaga accagaccat ggtatatagc agctatgaag
     9361 acacctggaa ttttacaaat gacagtagct tatccttctg taactaatga tgatattaac
     9421 atagcctttg taataacttt aaatgacaac aacaatatgc catatggtgt tattggaata
     9481 gatgtcacat tagaaacttt atcagaagta ttaaaaaacc agcagcttct gttcgaaggc
     9541 ttcatggaag tcgttgatga tcagaacatg atagtcatga cacctaaaga tgaaaacctg
     9601 tataccctga ataaacagga aacaaactac agacaactgg aacagacaga cacagtgaca
     9661 atagaaagaa acaaactgtt ctataaagta acatatacca aggaactgtg ggattcgaca
     9721 ttgatagctt atgctccgac taaagtagtt gagcgaagcc ttgtatccaa tcttatatcc
     9781 aaaatactct ggagctttat aatcctatta attatagaag caagcaatta cctgtttata
     9841 aagagataca tagataaacc tattaaggaa ctaataactt cacttaagga atcccagaag
     9901 actgatatac caaagaagat atcgctaaaa gctaaaggcg aattcaaaca gttcgaaaaa
     9961 cagtacaatg agcttgttga ttcattaaag cagaacgaaa atgaccttaa aaggataagg
    10021 aatataataa taacaagctt atcttcgtta acatccatga gggattttga aacagggctt
    10081 catctttaca gagcatctaa atatgtaaac cttttagcag aagcatacaa cgaaaagaat
    10141 ccagaagaac cgataccttc acagataata gatataatga cagagagcgc tcctttacat
    10201 gacataggca aaattgccat aagcgatgag attcttaaga aaaaaggacc attaacaaca
    10261 gaagaattcg aaataatgaa aaagcacact ctttatggca agcagatgtt tgagaagata
    10321 atgacaggaa ttgaaaacag agactttatt aaaacagctc ataatatgat ttattatcac
    10381 catgaaagat ggaacggaaa aggatatcct gaaaacctga gcaataacaa catacctctt
    10441 gaagcaagaa taatgtcaat agcagatgca tatgacgcaa tgacatccac aagagtctat
    10501 tcccctgcta tatcacatga agaagccaaa gcagaactta taaggtgcaa aggcacacag
    10561 ttcgacccca acctaataga cgtattccta tccatagaag actccataaa ggcagtggca
    10621 gaggcgtata aggagtag
//
LOCUS       DAOSXM010000069        10452 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2111
            NODE_4018_length_10452_cov_11.708666, whole genome shotgun
            sequence.
ACCESSION   DAOSXM010000069 DAOSXM010000000
VERSION     DAOSXM010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864178
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 10452)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10452)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:24:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,261
            CDSs (total)                      :: 2,229
            Genes (coding)                    :: 2,218
            CDSs (with protein)               :: 2,218
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 3 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10452
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4018_length_10452_cov_11.708666"
                     /isolate="AMR_MDS_2111"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..427)
                     /locus_tag="PLQ68_08500"
     CDS             complement(<1..427)
                     /locus_tag="PLQ68_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015508747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HXK72730.1"
                     /translation="MNTVLQYVRPYTKRITLGLSIKTIGTLMDLFIPWILSHIIDNVI
                     PTGNMKMVIAWGGGMILCAFLAIVGNIIANRMAASVARDTTRSIRHDLFTKISYLSNS
                     EVDRFTIPTLISRMTTDTYSVHHLVGMLQRMGIRAPILLL"
     gene            complement(431..2080)
                     /locus_tag="PLQ68_08505"
     CDS             complement(431..2080)
                     /locus_tag="PLQ68_08505"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HXK72731.1"
                     /translation="MTKKYSIGIDFGTLSGRTVLVDLSNGREIDVEVSNYPHAVMESQ
                     LPNGQKLPADFALQHPNDYLKVLSDTVPVILKRNFINAESVVGVGIDFTACTMMCVDN
                     NGTPLCLKDEFSSNPYSYVKLWKHHAAQKQADKINETAKNMGCDWLERYGGKISSEWF
                     FPKILETLENAYDVYENTYRFIEAGDWVVWMLTGNETHNACMAGYKAMWHKKDGFPKK
                     EFFKALDPRMENIIGTKVSEQVLPMGAKAGNINKLGEQLTSLSQGTAVAVSNVDAHVA
                     MPAAGITTADKMLMIMGTSTCHMVLGKEEKSVKGICGVVEDGIIEGYYGYEAGQSCVG
                     DHFDWFVKNCIPAEYRELAKEKGISIHKYLREKAITQKPGESGLLALDWWNGNRSVLV
                     DADLTGVILGITLATKPYEIYRALIEATAYGTRMIIENFEKNGVKIEQLYAAGGIADK
                     DEMMMQIYADVTNRTIRLSGSQQAPALGSAIFGALAGGYFKSITEAAQALGRIKDISY
                     TPNKENCIIYDKLYEEYSILHDYFGRGINNVLKRLKSIKGM"
     gene            complement(2172..3842)
                     /locus_tag="PLQ68_08510"
     CDS             complement(2172..3842)
                     /locus_tag="PLQ68_08510"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXK72732.1"
                     /translation="MKKRIFTSIFFASLLTLILTFASVSAILYNNAATSIKREVRNES
                     IYISEAIKSYNENIIDISSYLNSAGAKSLNRITLISSDGTVTYDNFAQIQTMDNHSDR
                     PEFIDAMKTGQGEITRFSDTLGESTYYYALKLNNNDVLRIASTTKSILGIVTESIVWF
                     ILIFIFIILISLLIARLLTKAVVKPINKLDLDNPLSNDTYEELSPLLLRMEKQKKEIK
                     NRMDELTKTRDDFNFITDNMSEGLVIFSEKGYVLASNKSANKILGISQKQYYLESCRD
                     SSYIELIESSLNGKPHTAKINRSGYTYQLFASPVKEESKNFATVLFITDITEKDQAEQ
                     RRKEFSANVSHELKTPLTSILGYAELISKEIAKKEDIPRFGELIHREAARLLQVIEDI
                     IKLSKLDEADLIKEFEDVRLDELCLTVINDLKQKASGYKVKCSFEGQKTIIKGYSTIL
                     YEMIYNLCDNAIVYNKAEGSVILTLEKTDNKAVLTVADTGIGIPQDHLPRVFERFYRV
                     DKSHSKDTGGTGLGLSIVKHAASLHDAKIDISSKINKGTKISISFNIN"
     gene            complement(3839..4513)
                     /locus_tag="PLQ68_08515"
     CDS             complement(3839..4513)
                     /locus_tag="PLQ68_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004614658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HXK72733.1"
                     /translation="MIYFVEDDSSIRELVVYTLNNTGFEACGMDNAKILYEKISKKKP
                     ELILLDIMLPEENGLEILNRIKSSPETSHIPVIMVTAKGTEFDKIKGLDSGADDYITK
                     PFGVMELVSRVKAVLRRTLKAPDSNEYEYEGIYINTKTHIVTSDNISIILTLKEFELL
                     HELMINKGIVLTRDNLLEKIWGYDYPGETRTVDVHIRTLRQKLGKNGESIKTVRGVGY
                     MIGGNI"
     gene            complement(4513..5160)
                     /gene="phoU"
                     /locus_tag="PLQ68_08520"
     CDS             complement(4513..5160)
                     /gene="phoU"
                     /locus_tag="PLQ68_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014117236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HXK72734.1"
                     /translation="MRNKFDMQLEELNNSLIGMGAMCETAIASAVKALLENDLTLAKK
                     VMESVKDINQAEKDIEAMCLKLILQQQPVAKDLRQISSILKMITDMDRIGVQAGDIAE
                     IVTFADLSESKDRVHIADMAKATIKMVSESIDAFVKKDVELANQVIEYDDVVDELFTK
                     VKDGIVNLISKDKKHGEQAVDIIMIAKYLERIGDHATNIAEWVVFSITGIHVEVS"
     gene            complement(5160..5909)
                     /gene="pstB"
                     /locus_tag="PLQ68_08525"
     CDS             complement(5160..5909)
                     /gene="pstB"
                     /locus_tag="PLQ68_08525"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004841676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HXK72735.1"
                     /translation="MDKFEIKKLNLYYDSFQALKNINISIQKNEITAFIGPSGCGKST
                     LLKTLNRMNDLIEGCKIQGEVLLDGTNIYGNIDINLLRKRVGMVFQKPNPFPMSVYDN
                     IAYGPRTHGIKSKAKLDELVERSLTDAAIFNEVKDRLKKSALDLSGGQQQRLCIARAL
                     AVEPEVLLMDEPTSALDPISTSKIEDLVLELKNRYTIVIVTHNMQQATRISDRTAFFL
                     LGEIIEMSDTESLFSNPKDIRTENYITGRFG"
     gene            complement(5902..6738)
                     /gene="pstA"
                     /locus_tag="PLQ68_08530"
     CDS             complement(5902..6738)
                     /gene="pstA"
                     /locus_tag="PLQ68_08530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015513234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HXK72736.1"
                     /translation="MKRAKSQTIFLRSIVYISAVITLMTVAGILIYILIKGVPHISWD
                     LFAFKYTSENVSLFPALVNTFTMTITALLMAAPLGIFSGIYLVEYANRNSKSVKVIRI
                     TTETLSGIPSIVYGLFGYLLFVATLGWGYSMLAGSLSLAIMILPVIIRSTEESLKSVP
                     DTYREGSFGLGAGKLRTVFRIVLPAAMPGILAGIILSIGRIVGETAVLIYTSGTVAQI
                     PKNLLGSGRTLSVHLYALWSEGMNTDSAYATAVVLVVTVIFLNAMSAYVAKKFTKGKK
                     NG"
     gene            complement(6735..7610)
                     /gene="pstC"
                     /locus_tag="PLQ68_08535"
     CDS             complement(6735..7610)
                     /gene="pstC"
                     /locus_tag="PLQ68_08535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004071606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HXK72737.1"
                     /translation="MNNLSNKKSKSIKENIMKAVFFASAFMSVISVLLICVFLFANGL
                     PAMKEIGFLEFLSGKEWRPGNDIYGIFPMIIGSLYVTAGALAIGVPIGVLTAVFMARF
                     CPAKLYKIIKPIINLLAGIPSVVYGFFGLMVIVPFIRNTFRVSGTSMLAASIVLGIMI
                     LPTIIGMSEAAIRAVPDSYYEGSLALGASHERSVFFSMLPAAKSGILSSIILGMGRAV
                     GETMAVVMVAGNQPIMPSGLLKGLRTLTANIVLEMGYAADLHREALIATAVVLFVFIL
                     IINLLFSAIKKGIKS"
     gene            complement(7603..8472)
                     /locus_tag="PLQ68_08540"
     CDS             complement(7603..8472)
                     /locus_tag="PLQ68_08540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HXK72738.1"
                     /translation="MKKVITIVISLILALAMFSGCTQTGFNQDTAITVISREEGSGTR
                     GAFIELFEIEQKNSEGVKVDRTIATADINSSTGVVLASVAQNMYSIGYISLGSLNDTV
                     KALQVDGADATVENIKSGTYKVSRPFNIAVKEGLSEVAQDFVNYILSKEGQAVVESAG
                     YISALTGDSYSGIKPSGTVTVAGSSSVTPVMEKLKEAYAVLNPNAVIEINQSDSTTGI
                     NSAIEGICDIGMASRTLKESEIEKGVTPTVIAIDGIAIIVNNENPLTGMTKDEVTKIY
                     IGEITSWSEIGNE"
     gene            complement(8662..10317)
                     /locus_tag="PLQ68_08545"
     CDS             complement(8662..10317)
                     /locus_tag="PLQ68_08545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010026149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HXK72739.1"
                     /translation="MKIETYNEPRFTDFRQLINHSADKYGKRIAFKIKTSNCYVYITY
                     KQLKERFYTLCNEFIALGLQGKRIAIIGKNSFEWVLSYLAAATVGIAVPLDKEIHPED
                     AKAFMESAECEAVCYGSSYADNIQTLLSENIKAFPFTEIMNMSSPINPIDYLAVDHIK
                     IEKYEMRILIFTSGTTGSAKGVCLSQYNLCSNIHSTVSVVRIRQNDTTLSILPLHHTY
                     ECTLDCLLILSRGAMITYSDGVTEIAKNFVEYSPSILVVVPALLKLLNKRITKSITKS
                     CPEKYKELYKTESLAEALRKTPYIIRKIICSKVRKSLGGRIRLFIVGAAELDTSLVDD
                     FAELGIRTLQGYGLTECSPLLAGNNDFYLNAASTGIAIPGVTLKIDNLNEAGVGEILA
                     KGENIMLGYYKDQEATDYVLRDGWFHTGDLGCMDPDSALYIKGRIKNVIVTQNGKNIY
                     PEELENRLMQFPEIGEVLVLGTVQKDDIQVKAKIFPNLDVLKEKLGRLPSGEDIHAAI
                     TNIIRDINKRMPTYKQIRETEILTDVLEKTTTQKIKRFGSNMK"
BASE COUNT         2984 a         2018 c         1705 g         3745 t
ORIGIN      
        1 ctaaaagaag aataggtgct ctaataccca ttctttggag cattccaacc agatgatgaa
       61 cagaatatgt atctgttgtc atacgggata taagtgtggg tatagtaaat ctgtctactt
      121 ctgagttaga aaggtaagat atttttgtga ataggtcatg ccttatactc cttgtagtgt
      181 ctcttgcaac tgaggctgcc attctgtttg cgataatatt acctactatt gccagaaaag
      241 cacatagaat catacctccg ccccaggcta taaccatttt catatttcca gttggtataa
      301 cattgtcaat tatatgagaa agaatccaag gaataaataa atccattaga gttccgattg
      361 tcttgatgct cagaccaagt gtaattctct ttgtgtatgg ccttacatat tgaagtactg
      421 tgttcatatt ttacatgccc ttaatacttt tcagtctttt aaggacgttg ttgattcccc
      481 ttccaaaata gtcatgaagt attgaatact cttcatacaa tttatcatat atgatgcaat
      541 tttctttgtt aggtgtataa gaaatatctt tgattcttcc taaagcctgt gcagcttctg
      601 ttattgattt aaaatatcct cctgctaatg ctccgaatat cgctgaacct aatgcaggtg
      661 cttgttgtga accggaaagt cgaattgttc tgttagttac atcagcataa atctgcatca
      721 tcatttcatc tttgtcagct attcctcctg ctgcatatag ttgctctatc tttacaccat
      781 ttttttcgaa attttcaata atcattcttg tgccatatgc tgttgcctca ataagtgctc
      841 tatatatctc gtatggttta gttgctaaag ttattccaag aataacacct gtcaagtcag
      901 cgtcaactaa cactgagcga tttccattcc accagtcaag agccagcaat ccgctttcac
      961 caggtttttg agttatagct ttttcccgta ggtatttgtg gatgcttatt cccttttctt
     1021 ttgccagttc tctatattca gcaggtatac agtttttaac aaaccagtcg aaatgatctc
     1081 caacgcagga ctggccagct tcatatccgt aatatccttc aatgattccg tcttcaacaa
     1141 caccgcatat gcctttgacg gatttttctt ctttaccaag taccatatga caggttgatg
     1201 ttcccataat cattagcatt ttatcagctg tggttattcc agctgcaggc atagcgacat
     1261 gagcgtcaac attgcttact gcaacagctg tgccctggct aagtgaggta agctgctctc
     1321 ccagtttatt tatattgccg gcttttgcac ccattggaag aacttgttca gacactttgg
     1381 ttccgattat attttccatt cgaggatcaa gtgccttaaa gaattctttc ttaggaaacc
     1441 cgtctttttt atgccacata gctttgtatc ctgccatgca tgcattatga gtttcgttac
     1501 cggttaacat ccatacaacc cagtcaccgg cctctatgaa tctgtaggta ttctcataaa
     1561 catcatatgc attttccagt gtttcaagaa ttttaggaaa gaaccattct gaagatattt
     1621 tgccaccata acgttccagc cagtcacagc ccatgttttt tgctgtttca tttatcttat
     1681 cagcttgttt ctgtgctgca tgatgtttcc aaagtttgac atatgaataa ggattactgg
     1741 aaaactcgtc ttttaaacat aaaggtgttc cattattatc aacacacatc attgtgcagg
     1801 cagtaaaatc aatacctact ccaacaactg attcagcgtt tataaagttt ctttttaaaa
     1861 taactggaac tgtatcggac aaaactttta gataatcatt tggatgctgt aatgcgaaat
     1921 cagcaggaag cttttgtccg ttaggtaatt gtgactccat tactgcgtga ggataattgc
     1981 ttacctctac atcgatttct ctaccattgc ttaaatcaac cagaacggtt ctgcctgaaa
     2041 gtgtgccaaa gtcaattcct attgagtatt ttttagtcat tgcagcataa tcctttcgct
     2101 tattaaagtt taaaggaaga cagatgaaat tgcaacatca gatagtgatt atatttataa
     2161 tcataatcta tttaatttat attaaaggat atagatattt ttgtaccttt attaattttg
     2221 cttgaaatat caattttcgc atcgtgtaaa gaggctgcat gctttacgat agacaatccc
     2281 agtcctgttc cacctgtatc ttttgagtgg cttttgtcta ctctgtaaaa tcgttcaaat
     2341 actcttggta agtgatcctg cggaattccg attcctgtat cagctacagt taagactgct
     2401 ttattatcag ttttttcaag agttaatatt acgcttcctt cggctttgtt atatacaata
     2461 gcattatcac acaagttgta tatcatttca taaagtattg tagaataacc tttgattatg
     2521 gttttttgtc cttcaaaact gcatttcact ttatatcctg atgctttctg tttaagatca
     2581 tttataactg ttaagcaaag ttcgtcaagt ctaacatcct cgaattcttt tataagatca
     2641 gcttcgtcca gctttgacaa tttgattata tcttcaataa cttgtaatag tcttgcagct
     2701 tcacgatgta taagttctcc aaaacgcggg atatcttctt tttttgcgat ttcttttgat
     2761 ataagttcag cataaccgag aattgatgta agaggggtct taagttcatg agatacatta
     2821 gcagaaaatt ctttccttct ttgttcagct tgatctttct ctgttatatc agtaatgaat
     2881 agaactgtag cgaaattctt agattcttct tttacaggac ttgcgaacaa ctgatatgta
     2941 taaccagatc tgtttatctt tgcagtatgc ggttttccat taagtgatga ttcaattagt
     3001 tctatatatg aagagtctct gcatgattca agataatatt gtttttgtga tatacctaat
     3061 attttattgg cgcttttatt tgatgctagc acatatcctt tttcagaaaa tattactaat
     3121 ccctcactca tattatcggt aataaaatta aagtcatcac gtgttttggt tagttcatcc
     3181 attctgtttt taatttcttt tttttgtttt tccattctta aaagcaaagg agagagttct
     3241 tcatatgtat cattggataa aggattatca aggtctagtt tgtttattgg ttttacaact
     3301 gctttagtta aaagtcttgc tattaacaaa gagattaaga ttatgaaaat gaaaatcaaa
     3361 ataaaccaga caattgattc agtaacaatt cctaatatgc tctttgtagt tgaagcgata
     3421 cgcaacacat cattattatt caatttcaat gcataatagt atgtagattc tcctaaggta
     3481 tcagaaaatc ttgttatttc accctgtccg gttttcattg catctataaa ctctggccgg
     3541 tctgaatgat tgtccatagt ttgtatctgt gcgaaattat catatgtaac tgttccatca
     3601 cttgatatca gtgttattct gttaaggctc tttgcacctg ctgaatttag atagcttgat
     3661 atatctataa tgttttcgtt atacgactta attgcttctg aaatatatat tgattcgttt
     3721 ctgacttccc gtttaatgga tgttgcagca ttgttatata agattgctga tacagatgcg
     3781 aatgttagta tcaaagtcag taaagatgca aagaatatac ttgtgaaaat acgctttttc
     3841 atatatttcc tccaatcata tacccaactc ctctgactgt ttttattgat tcgccatttt
     3901 tcccaagctt ctgacgtaaa gttcgaatgt gcacatcaac agttcttgtt tcaccggggt
     3961 aatcataacc ccatattttc tctaaaagat tatctctagt aagaacaata cctttattaa
     4021 tcattagttc atgcaatagt tcgaattctt tcaatgtgag tattatagaa atattatcag
     4081 aggttacgat atgtgtttta gtatttatat atataccctc atattcatat tcattggaat
     4141 ctggagcttt taaggttctt cgcagtactg ccttgactct tgataccagc tccattacac
     4201 caaaaggttt tgttatataa tcatctgcgc cactgtctaa acctttaatc ttgtcaaatt
     4261 cagttccctt tgcggtaacc ataattacag gtatgtgtga tgtttcagga gatgacttga
     4321 ttctgtttag tatttcaagc ccattttctt ccggaagcat aatgtctagt aatatgagtt
     4381 ccggtttttt ctttgaaatt ttctcatata gaatcttggc attatccatg ccgcaagctt
     4441 caaagccagt attgtttaat gtataaacaa caagttcccg tatactgctg tcatcttcca
     4501 caaaatatat cattacgata cttctacatg tatgccagta atagaaaata caacccattc
     4561 ggcaatatta gtagcatggt ctcctattct ttcaagatac tttgctatca ttattatatc
     4621 aactgcctgc tctccgtgct tcttgtcttt tgatattaga ttgacaatac catctttaac
     4681 ttttgtaaaa agctcatcga caacgtcatc atactctatt acctgattag caagctccac
     4741 atcttttttt acaaaagcat caatgctttc tgataccatc ttaatcgttg cttttgccat
     4801 atcagcaata tggactctgt ctttgctttc tgataaatct gcaaaggtta caatttctgc
     4861 aatatctcct gcctgaacgc caattctgtc catgtctgtt atcattttca atattgatga
     4921 tatctgacga aggtcttttg ctactggctg ctgttgtaat atcagtttta aacacattgc
     4981 ttctatatcc ttttcagcct gattgatatc ttttactgat tccattactt tcttagctaa
     5041 tgtaagatca ttttccagta atgcctttac tgcagaggct atggctgttt cacacattgc
     5101 tcccatgcct ataaggctat tgttcaactc ctctaactgc atgtcaaatt tattacgcat
     5161 catccgaacc tccccgttat atagttttct gtacgaatat cttttgggtt tgagaaaagg
     5221 ctttcagtat cgctcatttc tattatttca ccaagtagga aaaatgctgt tctgtcagat
     5281 attctggtag cctgttgcat attatgtgtg actatgacaa tagtatatct gttttttagt
     5341 tcaagaacaa ggtcttcaat cttggatgta gatattggat ctagtgcact agttggttca
     5401 tccataagaa gtacttcggg ttcaactgca agagctcttg caatacatag tctttgttgt
     5461 tggccacccg aaagatcaag agcacttttt ttcaatcgat ctttaacttc attaaaaata
     5521 gcagcatccg tcagcgatct ctccactagt tcatcaagct ttgctttgga ctttattcca
     5581 tgagttctcg ggccataagc gatattatca taaacactca tgggaaatgg gttaggtttc
     5641 tggaaaacca tacctacccg cttccttaat aggttgatat ctatatttcc atatatgtta
     5701 gtcccgtcta ataacacttc accttgtatt ttgcagccct ctataaggtc attcatcctg
     5761 ttaagggttt ttaaaagtgt agatttaccg caaccagagg gccctataaa agcagttatc
     5821 tcattttttt gtattgatat gtttatattc tttaaagcct gaaatgagtc ataataaaga
     5881 ttaagttttt taatttcaaa tttatccatt cttttttcct ttcgtaaact ttttggcaac
     5941 ataagctgac attgcattta agaatatgac agttacaacc aatacaacag ctgttgcata
     6001 cgcagaatca gtattcattc cttctgacca taatgcataa agatgtactg acagggttct
     6061 tcctgaacct aaaagatttt ttggtatctg tgctactgtt cctgatgtat atattagtac
     6121 ggctgtttcg ccgacaattc ttccaattga taatattata cctgcaagaa tacctggcat
     6181 ggcagctggt aagacaattc tgaaaactgt cctgagtttt ccagcaccca atccaaaact
     6241 gccttctcta tatgtgtcag gtacgctttt tagtgattct tccgtagaac gtattattac
     6301 cggcagtatc attatcgcta aagataagga accagcaagc atagagtaac cccagcctaa
     6361 tgttgcaaca aaaagaagat aaccgaacaa accatatact atagagggta tacctgaaag
     6421 tgtttctgta gttattctta taaccttaac acttttacta ttgcggttgg catactctac
     6481 aagataaatg ccagaaaata ttcctaaagg tgcagccatt aataaagctg ttatggtcat
     6541 tgtaaaagta ttaacaagag cagggaatag cgatacgttt tctgatgtgt atttaaatgc
     6601 gaacaaatcc cacgatatat gtggtactcc ttttattaat atatatatta aaatacccgc
     6661 aactgtcatt aatgtaatca cagctgagat atatactatg cttcttaaga aaatagtctg
     6721 actctttgca cgtttcatga cttaattcct ttcttaatag ctgaaaacag aagattaatt
     6781 attaaaataa atacgaatag aaccactgca gttgcaataa gtgcttctct gtgtagatct
     6841 gctgcatatc ccatctccaa aactatatta gcagtaagtg ttctaagtcc ttttaaaagt
     6901 ccggagggca ttataggctg attaccagct accattacga ctgccattgt ttcacctacg
     6961 gctcggccca taccaagtat tatactagac aggattccag attttgcggc tggtagcatt
     7021 gaaaagaaaa cgcttctttc atgacttgct cctaaagcca aagaaccttc gtaataactg
     7081 tcaggtaccg ctcttattgc agcttcagac attcctatta tagtaggaag tatcataatg
     7141 ccaagaacta tggaagctgc taacatgctt gttccgctga ctctaaaagt gtttctaata
     7201 aatggaacta ttaccataag tccgaaaaaa ccatatacta ctgagggtat tcctgcaagc
     7261 aggttaataa tcggtttaat tattttgtac aatttagcag ggcagaagcg tgccataaac
     7321 actgctgtca gaactccgat aggaacacct atcgctaatg ctcctgctgt tacataaagg
     7381 cttccgatga tcattgggaa tattccatat atatcatttc caggtctcca ctcttttcct
     7441 gaaagaaatt caagaaatcc aatttctttc attgccggaa gcccgttagc aaaaagaaat
     7501 acgcatatta gaagtactga tatgacggac atgaaggcgc ttgcaaagaa gacagccttc
     7561 atgatatttt cctttatgct cttgcttttt ttattgctaa gattattcat ttccaatttc
     7621 cgaccatgaa gttatctcac cgatatagat ttttgttact tcatcttttg tcattccagt
     7681 taaaggattc tcattgttaa ctattatggc tataccatcg atagctatta ctgttggtgt
     7741 tactcctttt tctatctcgc tttctttaag cgttcttgaa gccattccta tatcgcatat
     7801 tccctcgata gcgctgttaa tgcctgttgt ggaatcagac tggttaattt caataacagc
     7861 atttggattg agtacagcat atgcttcttt caatttttcc ataactggtg ttactgaaga
     7921 tgaacctgcc actgttactg tgccggaagg tttgattcct gagtaggaat cgcctgttaa
     7981 agctgagata tatcctgcgc tttctacaac tgcttgtcct tcttttgata aaatgtagtt
     8041 tacaaagtcc tgtgcaacct ctgacaatcc ttctttaact gcaatattaa aaggacgtga
     8101 aactttgtat gttccgcttt tgatattctc aactgttgca tctgctccat cgacctgtaa
     8161 agcttttaca gtatcattaa gtgatcctaa ggatatataa cctatggaat acatattctg
     8221 agctacagaa gctaaaacta ctcctgtgct ggaattgata tctgcggtgg cgatggtgcg
     8281 gtctactttt acgccttcag aatttttctg ctcaatctca aagagttcta taaaagcgcc
     8341 tcttgttcct gagccttctt cacgtgagat tactgtgata gcagtatctt ggttaaaccc
     8401 tgtctgcgta cacccgctaa acatagcaag tgctaatatt aagctaataa ctattgttat
     8461 aactttcttc attctttttt cctccaattt tctctttacc ataatcattt tctttttcat
     8521 ggctaaagaa taacctgtca atgtaaagac taagtacatt caattgttaa atgtatgtaa
     8581 aatatataaa aagaatgatg aaaaaaagcc ttctattaac tctgaaagaa ttaaaagaag
     8641 gctaatagat ctagtagtat gctatttcat attcgaaccg aaacgtttta ttttctgagt
     8701 tgttgttttt tccagtacat ctgttagaat ctctgtctcc cttatttgct tatatgtagg
     8761 catgcgttta tttatatccc ttattatatt agttattgct gcatgtatgt cttctccgct
     8821 tggaaggcga ccaagctttt ctttaagaac gtcaaggttt ggaaatattt tcgctttaac
     8881 ctgtatgtca tctttttgta cagttcccaa cacaaggact tctccaattt ccggaaattg
     8941 cattaaacgg ttttccagtt cttcaggata aatgtttttc ccgttctgtg tgacaataac
     9001 atttttaatt cttcccttta tatatagggc gctatcagga tccatgcatc cgagatctcc
     9061 tgtgtggaac catccgtcac gaagtacata atcagtagct tcctgatctt tgtagtagcc
     9121 cagcattata ttctcgcctt tagcaagaat ttcgccgact ccggcttcat tcaggttgtc
     9181 tattttaagt gtaactcctg gtatagctat tcctgtagaa gcggcattta ggtagaagtc
     9241 attattacct gcaagtaaag gcgagcattc tgtaagtcca tatccttgta atgtgcgaat
     9301 tccaagttct gcaaaatcgt ctactaaaga tgtatccaat tccgctgctc ctacaataaa
     9361 tagccttatt cttccgccaa gtgattttcg tacttttgaa caaattatct tacggatgat
     9421 atatggagtt ttgcgtaagg cttcagcaag tgactctgtc ttatatagct ctttgtattt
     9481 ttcagggcat gattttgtaa tcgatttcgt aattctttta ttaagcagct tcaacaaagc
     9541 aggtacaact acaagtattg aaggactgta ctctacaaag ttctttgcga tttctgtaac
     9601 tccgtcactg taggtaatca tagctcctct tgataagata aggaggcagt ctaatgtaca
     9661 ttcatatgta tgatgaagag gcagtattga taatgttgta tcattttgtc tgattctaac
     9721 cacagagact gttgaatgaa tattagaaca cagattatat tgtgataagc aaacaccttt
     9781 agcactgcct gttgttcctg aagtgaagat aaggatacgc atttcatatt tttctatttt
     9841 aatgtggtca acagccaagt aatctattgg gtttatcggt gaagacatat tcattatttc
     9901 agtaaaagga aaggctttta tattttcaga caataaggtt tgtatattgt cagcatagga
     9961 actaccataa catactgctt cacattctgc tgattccata aatgcttttg catcctctgg
    10021 atgaatttct ttgtctaaag gaactgctat tccaactgtt gcagctgcaa gataagataa
    10081 aacccattca aaactgtttt ttcctataat tgcaattctt tttccttgaa gccctaatgc
    10141 tatgaattca ttgcataaag tataaaaacg ctctttcaat tgtttatatg taatatatac
    10201 ataacagttt gaggttttta ttttaaatgc tatccttttt ccatatttat ctgcactgtg
    10261 gtttataagc tgacgaaagt ctgtgaatct gggttcattg tatgtttcta ttttcatgag
    10321 tatcctccct ggatagtttg tataaaggtt cttatgaatc tatatactca aagagattgc
    10381 tcctgagaaa aaacatctat acacatcaac tatatcatta tgcttatatt taaacaatgg
    10441 acttcaaaaa at
//
LOCUS       DAOSXM010000070        10245 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2111
            NODE_4124_length_10245_cov_9.876349, whole genome shotgun sequence.
ACCESSION   DAOSXM010000070 DAOSXM010000000
VERSION     DAOSXM010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864178
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 10245)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10245)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:24:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,261
            CDSs (total)                      :: 2,229
            Genes (coding)                    :: 2,218
            CDSs (with protein)               :: 2,218
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 3 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10245
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4124_length_10245_cov_9.876349"
                     /isolate="AMR_MDS_2111"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            76..1635
                     /locus_tag="PLQ68_08550"
     CDS             76..1635
                     /locus_tag="PLQ68_08550"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal 7TM
                     domain-containing protein"
                     /protein_id="HXK72740.1"
                     /translation="MTSINYLFVPYIWVTILLTTIILFLGLYVFRHKNVLGGRYFLLT
                     LFFAFIWIIAQGMEMSAQPLNLKIIWANIQYIPILCISCSYLLMTLKFTGNPILNKKW
                     FYPALFVVPFAINILLWIDGINHLFRQNVMLDTSGIFPTVAKDFGPLFYVIAVYNHII
                     ILVVCIVLLYAMRDKISIYKSQLQLFFIAVLIPVLANLLYITKAIPFKVDPTPIVFSL
                     SSIAIFINIFRFKLFNVMPIARSMIIENMKVGVIVLDLKGRFLDINPAAGRMLKLKSR
                     NIIGKQFSEKMNHYPECVHAFNDQQETVIELDSGYVNANSCHEISFSQIYGADQQPIG
                     WLIQIYDITSRKISERIVQHAASHDPLTGLPNRGYFKTLCSTMIENAKKDNKFLALAY
                     IDIDNYKSINDTYGHDVGDNVLDIIAKRLINALPENSIICRIGGDEFVAMFNIEDTVE
                     YLNKIGDDILESIKEKIPKIDANIIITASIGFCLYPEHGKEIDELLKKADKAMYKVKE
                     NDKNGYCIYNS"
     gene            1832..3022
                     /locus_tag="PLQ68_08555"
     CDS             1832..3022
                     /locus_tag="PLQ68_08555"
                     /inference="COORDINATES: protein motif:HMM:NF024702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat protein"
                     /protein_id="HXK72741.1"
                     /translation="MKFRKIFIFLLAITVILVGFTACVNDKFAVNVVCDEEQGITKGT
                     GSYKAGETVEVSAEAKDGYIFDKWSDNETSQKRTITVSQDVTYTALFKRLYKIEVQCD
                     TSKGTVTGGGEFVEGTQINISVSPKGDNALYQWNDGDDELNRTVTVTQDFTYIAEFID
                     ITNFIFEKETGTIKGFIGDPQKIIIPKYIYDIKVNEIGDWAFYDHKSLSIIRIPDSVT
                     RIGDRALSQTALITVEIPSSVTILGNSALSDNPNLISANIPNSVTEFGGFMFYGCKSL
                     KSVTLPSSIESLERETFSKCTSLTDITLPDSIKYIGDYVFYECGALSKVTMPKNLEKI
                     GYGAFSFCTSLSKISLPQTITDIKNDAFYQNNLTEISVVQGSYADTWCIKNKLSNVVV
                     YMEP"
     gene            complement(3037..3837)
                     /locus_tag="PLQ68_08560"
     CDS             complement(3037..3837)
                     /locus_tag="PLQ68_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008703983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HXK72742.1"
                     /translation="MNSQTNTAIITGGSSGIGFATAKAFLDKGYTVYNLSRRDFSCKG
                     INHVHCDVTDEAEVISSINKITKEVSSIDVLISCAGFGIAGAVEFTDLEEAKRQIDVN
                     FFGTVNVIKAVLPYMRNQCEGKIICVSSVAAEIPIPFQTYYSVSKSAINSYVLALANE
                     VKSFNIQVCAVMPGDIATGFTNARKTNLTGDDIYEGRITRSIQSMEKDELNGMSADYA
                     GRFISKIIEKKNLKPIYTIGFKYKLFVFLSKVLPQRIVSAIVAFLYAK"
     gene            complement(3834..5012)
                     /locus_tag="PLQ68_08565"
     CDS             complement(3834..5012)
                     /locus_tag="PLQ68_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018109619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HXK72743.1"
                     /translation="MDIFDKCFKYEIVDEIRQKGIYPFFHALQSRQDVEVIMEGKRKI
                     MLGSNNYLGLTTNENVVKAGIEAIEKYGTGCSGSRFLNGTLQMHIELENELADFLNKE
                     SALIFSTGFQTNLGIIAAIAGHNDYLICDRENHASIYDGCRLSYGTMLRYKHNDMLDL
                     ENQLKKVPDSSGAMIITDGVFSMGGDIARLPQIVELAKRYKARVMVDDSHSLGVIGDG
                     GRGTASYFNLEKEVDIYMGTFSKSLASLGGFMAADEKIIDYVRHNSRPFIFSASMTPS
                     SCAVALAALRELKNHPELPEKLMDISCYFRKKLDEAGIKTRQSLTPIIPIYTYELIAT
                     LTIAKELYEDGVYVNCSLPPATAPNECLLRTSLMATHTEELIDETVEIIKNVLGRHNL
                     "
     gene            complement(5268..>5494)
                     /locus_tag="PLQ68_08570"
     CDS             complement(5268..>5494)
                     /locus_tag="PLQ68_08570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72744.1"
                     /translation="IFFIAVNEFFTISLMDLSLSQLSWMLYAPLVALCVFVSFILRKK
                     QKIFAWAFMISSVIGLTVFHIILISVKFYY"
     assembly_gap    5495..5594
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5595..7684)
                     /locus_tag="PLQ68_08575"
     CDS             complement(<5595..7684)
                     /locus_tag="PLQ68_08575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HXK72745.1"
                     /translation="MYCINCGKQIPDGSKFCTNCGASQVNNQTPAGNQQQYQQSQNFQ
                     QPPPFQQPQQQYYAPGMPPQMPKKKKGGAIALVAILVIAAIIAGIIFIPKIFGPPEAT
                     KSPRAASIGFTQQNISVENTATIDNNGGVITLPSNSALSGSFVTIPATAVDQSVTVEV
                     GTVTGTYTNAPESISQTALHLDLGGYEDLNQPIMITFQYARNAVEAERVPVGLYIDDT
                     GKMTQMLTHSIDKENGQFTVLTFHASTFTYYILEDVDSYPDSYATGFLPSVDGFAEKN
                     TGSSIFSGGECYGMSTFAKWFYLNKKTTETGGFYNLFRSPQMGVSPTGDVITPQDVIA
                     TKAFQYTTKESSILWDTERMFSSYVEKDETGNITGYRMDNSVSVRCIMDAIFFWEEPV
                     EVGIYGEGGHSVLAYGYEKTSSKINIKIYDPNFPNDNTQKIVYDIASKTISTPSYPAG
                     FLDVRLTTTGYGTFTSVSDYEDILADAYNNFAGTIADLEITDPANGHTVETGTYAISG
                     SLDTLNMMGEQIGDMVEIISDNGQIFRQSLSKNGTSPSNFSVEVPLKSGENKFLFNII
                     YNDEKGNENYMSHDQYGWFIINSTVPANAIYVTLTWDGQPDVDLYVTNPAGETSYFEN
                     YTTSDGGYLDIDDTNSYGPEHYTLLASDSVMWGQGYEIKLHYYNGNGPTSYNVIVTVN
                     EGTRYEKTSYYSGI"
     gene            complement(7814..8446)
                     /gene="pdxT"
                     /locus_tag="PLQ68_08580"
     CDS             complement(7814..8446)
                     /gene="pdxT"
                     /locus_tag="PLQ68_08580"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012820112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HXK72746.1"
                     /translation="MLIGVLSVQGSFAEHIKVLDNMRIRHVQLRNFHDLSYIIDGLIL
                     PGGESTVQGKLIKESGMLSHLKRQINDGLPVLATCAGMILLAKDISNDEKPHLATLPI
                     KVRRNGFGRQIESFYTEENFEGLGTVPMTFIRAPYVEKVYKDTKVLATVKDRIVAVCY
                     KNQIALSFHPELNNDYRIHEMFIDMAQKRKSQVQSLIAVASLLTSENLQI"
     gene            complement(8447..9322)
                     /gene="pdxS"
                     /locus_tag="PLQ68_08585"
     CDS             complement(8447..9322)
                     /gene="pdxS"
                     /locus_tag="PLQ68_08585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005813582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HXK72747.1"
                     /translation="MNKTRYELNKELAQMLKGGVIMDVTTPEQAKIAEEAGACAVMAL
                     ERIPADIRAAGGVSRMSDPKMIKEIQNAVSIPVMAKCRIGHFVEAQILEAIEIDYIDE
                     SEVLSVADDVYHIDKTKFKVPFVCGARDISEALRRISEGAAMIRTKGEPGTGDIVQAV
                     RHMRMINRSILSIASMKEDELFEEAKKLCVPYDLLLYVHNNRKLPVVNFAAGGVATPA
                     DAALMMQLGAEGVFVGSGIFKSNDPAKRAKAIVNAVTNYNNSSILAQVSEDLGGAMVG
                     INEQEIQLIMSERGI"
     gene            9428..>10245
                     /locus_tag="PLQ68_08590"
     CDS             9428..>10245
                     /locus_tag="PLQ68_08590"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HXK72748.1"
                     /translation="MLTYNIDNSSRYPLYEQIYMKIKKDISDGKLITGEKLPSKRELA
                     KHLQISIITVENAYAQLIAEGYIYSQEKKGYYISDITKMMYSQMHEKKSQQIVKEKKE
                     YFIDFVSNSIDVQGFPITIWSRLVREILSKENQKNILRSAPSQGLYELREAISKHLYQ
                     FRGMDVKPDNIVIGSGTEYLYNLLIQLLGNDLVFAVEDPGYAKPYLIYRSNKTSCAHI
                     SMDEQGIDMSALNDSNADIVHITPSHHFPTGIVMPISRRYELLNWAYNKKQRYI"
BASE COUNT         3329 a         1768 c         1690 g         3358 t
ORIGIN      
        1 acagctgtat tgattttatt aagaaaggtg atataattta cataaattag ttattgtatt
       61 ttgaaaggtt aaagggtgac atccattaac tatttattcg ttccatatat atgggtaacc
      121 atattactta caacgattat attattccta ggactttatg ttttcagaca taagaatgtt
      181 ttaggcggga gatactttct tttaacactt ttttttgctt ttatatggat aattgctcag
      241 ggcatggaga tgtctgcaca gccgttgaat cttaaaatta tttgggccaa tattcaatat
      301 attcctatac tatgcatatc ctgctcatac cttttaatga cattgaaatt tactggaaat
      361 ccaattctga ataaaaaatg gttttatccg gctttgtttg tagtcccttt tgcaataaat
      421 attttacttt ggattgatgg tataaaccat cttttcagac agaatgtaat gttggataca
      481 tcaggaatat ttccaactgt ggctaaagat ttcgggcctc tattttatgt tattgcagta
      541 tataatcata taataattct tgttgtatgc atagtattgt tatacgcaat gagagataag
      601 atatctatat ataaaagtca gttacagtta ttttttattg cagtattgat acctgtatta
      661 gctaatttat tgtatataac aaaagctatt ccattcaaag tggatccaac acccatagtt
      721 ttcagtttgt catctatagc tatctttatt aatatattcc ggtttaagct attcaatgtt
      781 atgcctatag ctagaagcat gattatagaa aatatgaaag tcggtgttat agttctggat
      841 cttaaaggca ggtttttgga tataaaccct gcagcaggaa gaatgttaaa acttaaaagc
      901 agaaatatta taggaaagca gttttcagaa aaaatgaatc actatcctga atgtgttcat
      961 gcatttaatg accagcagga aacagttatt gaattagatt ccggatatgt aaatgctaat
     1021 tcctgtcatg agatatcatt tagccaaatt tatggtgctg accagcaacc cataggttgg
     1081 ttaattcaga tatatgatat aacaagtcgt aagatatcag aaagaattgt tcagcatgca
     1141 gcatcacatg atcctttgac cggcttgcca aatcgcgggt atttcaaaac actgtgcagt
     1201 actatgattg agaacgcaaa aaaagacaat aaatttctag cattagctta tatagatatt
     1261 gataactata aaagtataaa tgacacatat ggacatgacg taggagataa tgtacttgat
     1321 ataatagcta aaagactaat aaatgcttta cctgaaaatt ctataatctg caggattggc
     1381 ggagatgaat ttgttgcaat gtttaatata gaggatactg ttgaatattt gaacaaaatt
     1441 ggcgatgata ttcttgaaag cataaaagag aaaattccaa aaattgatgc taatattata
     1501 attacagcaa gtataggttt ttgcttatat cctgaacatg ggaaagaaat tgatgaatta
     1561 cttaaaaaag ctgataaagc aatgtataaa gtaaaagaga atgataaaaa cggatactgc
     1621 atatataata gttgattagt tcttattgat tcacatactt attagaaata aacattttcc
     1681 aatcatcata ttgtaaaatc ggatttaaat gatatatggt ttcaaaatag aatttgataa
     1741 atttaatata tagagaaaaa tcacacagta caagaaagtc attatttatt gtataattaa
     1801 tatatagttt attattatga agggggtaca aatgaaattt agaaaaatat ttatattttt
     1861 attagctata acagttattc ttgtaggatt tactgcttgt gtgaacgata agtttgcagt
     1921 caatgtagta tgtgatgaag aacagggaat aacaaaaggg acaggttcat ataaagcagg
     1981 agaaacagtt gaagtttctg ctgaagcaaa agatggttat atatttgaca aatggtcaga
     2041 taacgaaacc agtcagaaac gtacaattac agtttcacag gatgttacct atacagcttt
     2101 atttaagaga ttatataaaa tcgaagttca atgtgacaca agcaaaggta ctgtaactgg
     2161 tggcggagaa ttcgttgaag gcacgcaaat taacatatct gtatcaccta aaggggataa
     2221 tgccctgtac caatggaatg atggagatga tgaactgaac cgtacagtaa cagtaactca
     2281 ggattttact tatattgcgg aatttattga tataactaat ttcatttttg aaaaagagac
     2341 agggactata aaaggtttca ttggagatcc tcagaaaata attattccaa aatatatcta
     2401 tgatataaag gttaatgaaa taggcgactg ggctttttat gatcataaat cattatcaat
     2461 tatcaggata cctgacagtg ttacaagaat aggagacaga gcattatcgc aaactgcact
     2521 tataacagtt gagattccta gcagtgttac tattttaggt aattctgcat tgtctgataa
     2581 tcctaatctt atatctgcaa atattccaaa ttctgttact gagttcggag gatttatgtt
     2641 ttatggatgt aagtcattaa aaagcgtaac tctgccatca tcaattgagt ctttagaaag
     2701 ggaaacattt tctaaatgta catcgctaac tgatataaca ttacctgact caataaaata
     2761 tataggtgat tatgtgtttt atgaatgtgg tgctcttagc aaagtaacaa tgccaaaaaa
     2821 tctagaaaaa attgggtatg gagcattttc attctgtact tcattaagta agatatcctt
     2881 acctcaaact attacagata ttaagaatga tgcattctac caaaataatc ttacagaaat
     2941 aagtgtagta caagggtcat acgctgatac atggtgcatc aagaataaat tgtcaaatgt
     3001 agtagtttac atggaaccgt aagatttcat taatcgctat ttagcatata gaaatgctac
     3061 tatagcgctt actattcttt gcggtaatac tttagaaaga aaaacgaata acttatattt
     3121 aaaacctatt gtgtaaatag gttttaagtt tttcttctca ataattttac taataaatct
     3181 tccagcgtaa tcagcagaca ttccgtttag ttcgtctttt tccatagact ggatagagcg
     3241 tgttattctg ccttcatata tatcatcacc tgttaaattg gtctttcgtg cgttggtaaa
     3301 tccggtagct atatcaccag gcattaccgc gcatacttgt atattgaaac ttttcacttc
     3361 attcgccaaa gctaatacat aggaatttat tgcagatttt gacacgctgt aatatgtttg
     3421 aaaaggtata ggaatttctg cagcaacaga tgaaacgcat attatctttc cctcacattg
     3481 atttctcata tatggaagta cagcctttat aacattgacc gtgccaaaaa aattcacatc
     3541 tatttgcctt tttgcctcct ctaaatctgt gaactcaact gctcctgcta ttccaaaacc
     3601 agcacatgaa atcaaaacat caatactgct aacttctttt gtaatcttat ttattgagct
     3661 tatgacttca gcttcatctg taacatcaca atgaacatga tttatgccct tacaactgaa
     3721 atccctgcgg cttagattat aaacagtata tcctttatcc aaaaaagctt ttgctgtagc
     3781 aaaaccgata ccgctgcttc cacctgttat tatggcagta tttgtttgac tattcacaga
     3841 ttatgccttc ctaatacatt ttttataatt tcaactgttt catcaattaa ttcttctgta
     3901 tgtgtggcca ttaggcttgt tcttaataag cattcgtttg gagctgtagc aggcgggagt
     3961 gagcagttaa catacactcc atcctcataa agctcttttg caattgtgag tgtagcaata
     4021 agttcataag tgtaaatagg aataataggt gttagagatt gtcttgtctt tatacctgct
     4081 tcatccagtt tttttctgaa gtagcaagat atgtccataa gtttttcggg cagttctggg
     4141 tgatttttta attcacgtaa tgcagctaaa gcaactgcac agcttgaagg agtcatagaa
     4201 gctgagaaaa taaatggacg agaattatga cggacataat caataatctt ctcatctgca
     4261 gccataaacc ctcctaagct tgctaaagac ttagagaatg tacccatgta gatgtcaact
     4321 tctttttcta aattgaaata acttgcagta cctcttccgc catctccaat aacgcctagg
     4381 gaatgcgaat cgtctaccat aactctggct ttgtatcttt tagcaagttc cacaatttga
     4441 ggaagtctcg ctatatcacc acccatagag aatactccgt ctgttatgat cattgctcct
     4501 gatgaatcag gaactttttt taactgattt tccaaatcta gcatgtcatt atgtttatat
     4561 cttaacatag ttccataaga taatctgcat ccatcataaa tggaagcatg attttccctg
     4621 tcacaaatca ggtaatcatt gtgaccggct atagcggcaa taattccaag attagtctga
     4681 aaacctgtgg agaaaataag ggctgattct ttatttaaaa aatctgcaag ctcattttcc
     4741 agttcaatgt gcatctgcaa agtgccatta aggaaacgtg aaccggaaca gcctgtacca
     4801 tatttttcaa ttgcttcaat gccagcttta acaacatttt catttgtagt aagtccaaga
     4861 taattattag agccaagcat aatttttcgt ttgccttcca taataacttc tacatcctgt
     4921 cttgactgaa gagcatggaa aaaagggtat atgccttttt gccttatttc atctactatc
     4981 tcatatttaa aacatttgtc aaaaatatcc attactgcct cctgcagatt tcaaagaaag
     5041 tctgcttata attttctgac gatattttct atgcatatga agaatgcagt acatacttat
     5101 ttcttcataa attttaaccc cactaaatat ttattaccaa gtaataatat cttcgtgatt
     5161 ttacactata atatgctatt ttgtcaatat tttgtatatg aaaaaacctg attatgagaa
     5221 ttatcacaag caggtttaag attttataat agagcttcgt aatattatta atagtaaaac
     5281 ttaacagata ttaaaatgat atgaaaaact gtcagtccga taacagaaga gatcatgaaa
     5341 gcccaagcga atatcttctg ttttttacgc agtataaatg atacaaaaac acacaatgca
     5401 accaaaggcg catataacat ccaggataac tgcgaaaggg aaagatccat tagtgatatt
     5461 gtgaaaaact cattaacagc tatgaagaaa ataannnnnn nnnnnnnnnn nnnnnnnnnn
     5521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5581 nnnnnnnnnn nnnnataata ccgctgtaat atgaagtttt ctcatatctt gtaccttcat
     5641 ttacagttac tattacattg taactagttg gaccatttcc attataataa tgaagtttaa
     5701 tctcatatcc ctgtccccac ataacgctat cagaagcaag cagagtataa tgttcaggtc
     5761 cgtaactgtt tgtatcatct atatcaagat aaccgccgtc tgatgttgtg taattttcga
     5821 agtatgatgt ttcaccagca ggatttgtga cataaaggtc aacatcaggt tgaccgtccc
     5881 aggttaaggt aacatagatt gcatttgccg ggacagtaga attaataata aaccatccat
     5941 actgatcatg agacatataa ttctcatttc ctttttcatc attgtagata atattgaaca
     6001 aaaacttatt ttcaccgctc ttaagaggaa cttcaacaga aaaattagaa gggcttgtac
     6061 catttttgga taagctttgt ctgaatattt ggccattatc actgatgatc tctaccatat
     6121 caccaatttg ctcacccatc atattcaaag tatctaaaga gcctgatatt gcatatgtac
     6181 ctgtttcgac agtatgaccg tttgctggat cggttatctc caaatctgcg attgttcctg
     6241 caaagttatt atacgcatca gctaagatgt cctcgtaatc gcttacgctg gtaaatgtgc
     6301 cataacctgt agtggtcagt cttacatcaa gaaaaccagc agggtatgaa ggtgtggata
     6361 tagtttttga ggctatgtca taaacaatct tctgagtgtt atcattaggg aagttgggat
     6421 cgtaaatttt aatattgatt ttactactgg ttttttcata accataagct aatactgagt
     6481 gtccgccttc accatatatg cctacttcaa caggttcttc ccagaagaaa atagcatcca
     6541 ttatacaacg tacggaaaca gagttatcca ttctgtatcc tgtaatattt ccagtttcat
     6601 ccttctcaac ataacttgag aacattcttt cagtatccca caatattgat gattcttttg
     6661 ttgtgtattg aaaagctttt gtggcaatta catcctgagg agttataaca tctcctgtag
     6721 gcgaaactcc catctgagga gatctgaaca ggttatagaa acctccagtt tcagttgtct
     6781 tcttgttaag atagaaccac ttggcaaatg tactcatacc ataacattca cctccggaaa
     6841 aaatacttga gccagtattc ttttctgcaa acccgtcaac gctcggcaag aagcctgtcg
     6901 catatgaatc aggatatgaa tccacatcct caagtatata atatgtgaat gttgatgcgt
     6961 ggaaggttaa aacagtaaac tgcccgtttt ctttgtcaat gctgtgagta agcatttgtg
     7021 tcattttgcc tgtatcgtct atatatagcc ctacaggaac tctttcagct tcaacagcat
     7081 ttcttgcata ctggaacgtt atcatgatgg gttggttaag atcctcatag cctcctaaat
     7141 ccagatgaag agctgtttgt gaaattgatt ctggagcatt agtgtaagtt cctgttacag
     7201 ttcctacctc aacagttaca gattggtcta ctgccgttgc aggaattgtt acaaaactgc
     7261 ctgataatgc gctgtttgaa ggaagtgtta tgactcctcc gttattatct attgtagcag
     7321 tattctcaac tgaaatgttc tgctgagtaa aacctatact tgctgctcta ggtgatttag
     7381 ttgcttccgg cgggccgaaa atttttggaa taaaaattat acctgcaata atagctgcta
     7441 taacaagaat agctactaaa gcaattgcgc cacctttttt ctttttaggc atttgtggag
     7501 gcatgccagg agcataatac tgctgctggg gttgctgaaa aggtggaggc tgttgaaaat
     7561 tctgagactg ttgatactgc tgctgatttc cagcaggtgt ctggttgttt acttgagaag
     7621 ctccacagtt tgtgcagaat ttggagccat ctggtatctg cttaccgcaa ttgatgcaat
     7681 acatgattct accccctaaa atgtattctt cgaataacta aaaatcaaaa tccaatttgt
     7741 tttaccacat aatgaatata tataaatttg caaaaagtgt caacaaaaat ttacaccttt
     7801 tattgccaaa tctttatatc tgaagatttt cagaagttaa caaagaagct actgcaatta
     7861 aagattgaac ctggcttttt cttttttggg ccatatcaat aaacatctca tgaatcctgt
     7921 aatcattatt caattctgga tgaaatgata aagcaatctg gtttttataa caaactgcaa
     7981 ctatacggtc tttgacagta gcaagcacct tagtatcttt atatactttt tcaacatatg
     8041 gtgctcttat aaatgtcata gggactgttc ctaagccttc aaaattctct tcagtataaa
     8101 aactctctat ttgtctgcca aatccatttc ttctgacctt tataggaaga gttgccaagt
     8161 gtggtttttc atcgtttgaa atatcttttg ctaaaagaat catccctgca caagtggcta
     8221 atactggaag accatcatta atctgtcttt taagatggct taacattcca gattctttta
     8281 ttagttttcc ctggacagtg ctttcaccgc cagggagtat aagcccatca atgatatatg
     8341 acagatcatg aaaatttctt aactgaacat gcctgatcct catgttgtcc aaaactttta
     8401 tatgttctgc aaaagaaccc tgaactgata acactcctat taacatttaa atacctcttt
     8461 cagacattat tagctgaatt tcctgctcgt ttatacctac catcgctcca ccaagatcct
     8521 cagaaacttg tgccaaaata gaagagttat tatagttagt gactgcatta actattgctt
     8581 ttgcacgttt ggcaggatca tttgatttaa atattcctga acctacaaaa acaccttcag
     8641 ctccaagttg catcattaaa gctgcatctg ctggtgttgc tacaccgcct gctgcaaaat
     8701 taactacagg aagctttctg ttattatgta catagagcaa taaatcatat ggtacacaaa
     8761 gcttctttgc ctcttcgaac aactcatctt ctttcattga ggcaatactt aaaatagagc
     8821 ggtttatcat acgcatgtgt cgtaccgcct gaactatatc tccagttcct ggttctcctt
     8881 tagttcttat catagcagct ccttcactta tccttctaag agcttcagat atatctcttg
     8941 caccgcatac gaaaggaact ttgaattttg ttttatcaat atgatataca tcatcagcaa
     9001 ccgataaaac ctcagattca tcaatatagt ctatttcaat agcttccaat atctgtgctt
     9061 caacaaaatg tccaattcgg cattttgcca taactggtat agatactgca ttttgaattt
     9121 ctttaatcat ttttggatca ctcatacgag atacaccacc tgctgccctt atatctgcag
     9181 gaattctctc tagggccatt acagcacatg ctcctgcttc ttctgcaatt tttgcctgct
     9241 caggtgtggt aacatccatt ataactccgc ctttaagcat ctgtgctaat tctttgttaa
     9301 gctcatatct tgttttgttc atcttttaag cctccgaaat tttatttcat taataacata
     9361 tttcaaaatt gaacatattg aaataaccaa aacaataata tatgataaga ccagattgga
     9421 gacagatatg cttacataca atatagataa cagttccaga tatccattat atgaacagat
     9481 atatatgaag ataaaaaaag atatttcaga tggtaaatta attactggtg agaaattgcc
     9541 ttctaaaagg gaactggcaa agcatcttca aatatccata atcactgttg aaaatgcata
     9601 tgctcagctt atagctgagg gatatatata ttctcaagag aagaaaggat attacatttc
     9661 agatataacc aaaatgatgt atagtcaaat gcatgaaaag aagagtcagc aaatagttaa
     9721 agaaaagaaa gaatatttta tagactttgt ttccaactca atcgatgtgc agggatttcc
     9781 aattacaatt tggagtcgtc tggtaagaga aatactgtcg aaagaaaacc agaagaatat
     9841 actaagaagc gctccttctc aaggactgta tgagcttcgt gaagctatat caaaacacct
     9901 atatcaattt cgtggaatgg atgtgaaacc cgacaatatt gtaattgggt ctggaacaga
     9961 gtatttatat aatcttctta ttcagctttt aggtaatgac ttagtatttg cagtagaaga
    10021 ccctggatat gctaagccat atcttatata cagaagcaat aaaacttcat gcgctcatat
    10081 aagtatggat gaacagggaa ttgatatgag tgcattaaat gattcaaatg ctgatatagt
    10141 acatattact ccatctcacc actttccaac aggaattgta atgccaataa gcagaagata
    10201 tgaattactg aactgggcat ataataaaaa acaaagatat ataat
//
LOCUS       DAOSXM010000071        10237 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2111
            NODE_4127_length_10237_cov_10.360244, whole genome shotgun
            sequence.
ACCESSION   DAOSXM010000071 DAOSXM010000000
VERSION     DAOSXM010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864178
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 10237)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10237)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:24:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,261
            CDSs (total)                      :: 2,229
            Genes (coding)                    :: 2,218
            CDSs (with protein)               :: 2,218
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 3 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10237
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4127_length_10237_cov_10.360244"
                     /isolate="AMR_MDS_2111"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            498..>2178
                     /locus_tag="PLQ68_08595"
     CDS             498..>2178
                     /locus_tag="PLQ68_08595"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013433216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HXK72749.1"
                     /translation="MYYSKMLIPTIRENPQEAEAISHKLMLKAGLIRRLASGIYTFLP
                     LGYKALRNVERIIRQEMESKGALELFMSALIPKELLEESGRWEVFGPEMFRLKNREER
                     DFCLGPTHEESFTVTARNEIKSAKNLPLTLYQIQTKFRDELRPRFGIIRCREFMMKDA
                     YSFDKDELGLDISYSAMYDAYTKIFKRCGLDIVIADADSGAMGGSGSQEFMVVNDIGE
                     DEIVVCSSCGYAANIEKAEAVTIKRNVNETPLEKELIHTPDAKTIDDLTSLFSCSSQQ
                     FAKSLVLIADSEPVMAVLRGDRELSLNKLASILGCSNLDMADELTIKNLTKADVGFAS
                     PVGIKCKVICDNELMNMVNFITGANKTDYHYKNVNLSDFSSYEFHDIRLVTDNDLCPR
                     CNEPLKIVKTIEVGHIFKLGDKYSKALDCNYTDESGERKPMIMGCYGIGLGRTLAAVI
                     EQNNDEHGIIWPMEVAPYKVYILPTLTDDPLISEMAKKMHDELVSNGIDVILDDRNER
                     PGVKFNDADLLGIPLRVTIGRNAKDGLVEFKKRSEKEVSLITYSKAIEEILS"
     assembly_gap    2179..2278
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2279..2625)
                     /locus_tag="PLQ68_08600"
     CDS             complement(<2279..2625)
                     /locus_tag="PLQ68_08600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004629565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HXK72750.1"
                     /translation="MLGKDLKDKLDLIYKEYTEVLSKTDSQYNFQFVFSDGQMNKGVM
                     LIGEAPGKDEVIQRKPFVGLAGGKLKLFMNSLGLNREDLFVTNVIKYRLYSINAKTGR
                     MKNRPAKKQEILL"
     gene            complement(2600..3166)
                     /locus_tag="PLQ68_08605"
     CDS             complement(2600..3166)
                     /locus_tag="PLQ68_08605"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015908226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HXK72751.1"
                     /translation="MTNDILTSEWQELTKQCAECKRCSLWENRKNVVIGRGSKTADLM
                     LVGEAPGEEEDKQGRAFVGKAGQLLSMALSSLSIEEKDYYICNVIKCRPQNNRTPYEE
                     EIESCMPYLDRQIDLVKPKIIILLGNTAASSLIRKNIRITQQRGTWDVYKGIPVIMTY
                     HPAALLRDESKKIDMWRDIKSAWKRLKG"
     gene            complement(3316..4269)
                     /gene="whiA"
                     /locus_tag="PLQ68_08610"
     CDS             complement(3316..4269)
                     /gene="whiA"
                     /locus_tag="PLQ68_08610"
                     /inference="COORDINATES: protein motif:HMM:TIGR00647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HXK72752.1"
                     /translation="MSYTTIVKKELSYKDVTKNCCEKALLSAIVRLEGRFIKERNGYY
                     SLNINTQDNTEARWFIFAMNRIYSLHSDIHMKKNKLNDNTIFCVKTEATYAASVVYSD
                     LGLMEVDEQNNGIFSDIAELTESTCCRYSYYEGAFLSSGTIKDPEKGYYLEIASKNSY
                     EIERLYNMLIKESLVNAKRSKRRKTQYVYFNNSEDISTFLNMIGAHNALYDYENKRIV
                     KSLRNDTNRMLNCDEYNSDKSVETKIRQINAIKYIQKNKKFDVLNGKLKEAAMLRLNE
                     DLTEYNEIAEHMSVKVSKSTVQKRLAKIEQIARQLGYESKE"
     gene            complement(4269..5153)
                     /gene="rapZ"
                     /locus_tag="PLQ68_08615"
     CDS             complement(4269..5153)
                     /gene="rapZ"
                     /locus_tag="PLQ68_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008908828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HXK72753.1"
                     /translation="MDLFIITGMSGAGKSYALKTMEDLGFYCIDNIPPSFIPQFVKMV
                     ADNKKIEKVALVADIRGAEPLENILSALNTLDEQGYVYKLIYLVASDVMLLKRYRETR
                     RHHPMENDSLNTQQAIQMEREEFGEISKRADHVIDTSQLLTKEFKQELAEIVLGGPLK
                     ETLIITLVSYGVKYGNPVDCDLIFDLRFTPNPYYIDEMKEMTGKEKPVIEYVLGQKAT
                     QDFLKMLTEMIEFLIPLYIKEGKTSLVIGLGCTGGRHRSVVIADEFAKRLEKNKHRVT
                     IQHRDIEKDAIVKEYGKR"
     gene            complement(5153..6061)
                     /gene="murB"
                     /locus_tag="PLQ68_08620"
     CDS             complement(5153..6061)
                     /gene="murB"
                     /locus_tag="PLQ68_08620"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010480.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HXK72754.1"
                     /translation="MKGLTRNLEALLKKYDFIRYNEPMSNYTAYKTGGSADILAHVEN
                     TDQLISLLKDLRSLNMPAYIFGNCTNILVTDKGIRGAVILTQKLNGIKAEENIVTCQS
                     GISMAKLAGFAYENSLTGAEAISGIPGSVGGAVYMNAGSYGTQMEDIVISSTYLDEDL
                     NIHTLSLKEHEFGCRRSFYTDKPFIILEAKFSFNKGDKESIKKDMIEYARRRKSSQPL
                     EYASCGSVFKRPLGHFAGKLIEDAGLKGYTVNGASVSTKHAGFIINTGNATSKDILTV
                     MDTVKTVVYNKYGVDLEPEVRIIGEE"
     gene            complement(6024..6980)
                     /gene="hprK"
                     /locus_tag="PLQ68_08625"
     CDS             complement(6024..6980)
                     /gene="hprK"
                     /locus_tag="PLQ68_08625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004616601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HXK72755.1"
                     /translation="MDLSDDYDDSIHSISVNRIIERYQLDKVYIPENIDDRTISVIEV
                     YRPGLQLAGYLEGFKPERVQLFGRTEMNFLGTLSRTERLKKLEDYMHEDIPCIIISNS
                     LPVFDEIVKAARIKRVPVLRTFETTSVFLAMLISYLNLELAERITIHGELLELYGVGI
                     LITGHSGVGKSETALELVKRGHRLIADDVVEIRKVSQTTLLGTAPEIIRHFMELRGVG
                     IIDVKGLYGAGSVKDIDSIDLVINIERWDDNKKYDRIGVDEVYTEYLGIKIPSLTLPI
                     MPGRNMPILMEVAAMDFRQKKMGYNAAKEMYERVNKKFGSTP"
     gene            complement(6964..>7173)
                     /locus_tag="PLQ68_08630"
     CDS             complement(6964..>7173)
                     /locus_tag="PLQ68_08630"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HXK72756.1"
                     /translation="DICYVAEGKTDAFLTSNLKPWDYAAACCILKEAGGICTDWKGEL
                     PSMKHKSDILATNSLLQEVLYGFVR"
     assembly_gap    7174..7273
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(7386..7673)
                     /locus_tag="PLQ68_08635"
     CDS             complement(7386..7673)
                     /locus_tag="PLQ68_08635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72757.1"
                     /translation="MKRLSVLILIIALLLTLSACQTYLFGEYEDELSGRKYEFMNNEF
                     ILTEGDSEITGTYSIKKDSITFKYDDTEVTYSYERDGTSAIIDGVEYKRGL"
     gene            complement(7713..9086)
                     /locus_tag="PLQ68_08640"
     CDS             complement(7713..9086)
                     /locus_tag="PLQ68_08640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72758.1"
                     /translation="MKFQTFLASEIATLDSNIYSGGGTDVTSALQKILDKADNNNGVH
                     LIMDGAGLITGLDVKSNTTIECLSKDCGFFLKDGSDRSLIDLKERSYTERLTRNVTLI
                     GGTYNHNNKGQQHHVPITDEKYRFGIEGISDDMGASRLVVAFEFFGIEDLVVRDITIR
                     NQRAWSVMCVNFKNVTMENIYIDLPNWMKGQNQDGLHFWGPGQFLTLRNIKGRAGDDF
                     IALGPDEHDLVSSITDVLIDGVHLENADQAIRMLSRAKGRLDRVTVKNVTGTYRSYGF
                     FINPWFPGDGFGNYGHIYFENIDLRPLDHVYPYRTATLFDIGGNFDCITFKNIHHQDP
                     SDDRPLFIFGLPFHRNDLNYADDFRPCIKNAVIDGLTILQSEKDSKVEEYIQIYDRVE
                     NLFLKNVIVSSDDNCEPVETFIGFKTFKNCERKGKIKNLVTHDVYMPNVKTLLKEPQQ
                     VEQISKT"
     gene            complement(9279..>10237)
                     /gene="groEL"
                     /locus_tag="PLQ68_08645"
     CDS             complement(9279..>10237)
                     /gene="groEL"
                     /locus_tag="PLQ68_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006314526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HXK72759.1"
                     /translation="ISNIQEILPLLEQIVQSGKKLIIVAEDVEGEALATLVVNKLRGT
                     FNCVAVKAPGFGDRRKAMLEDIAILTGGQVISEELGLDLKETKVDMLGRARQVKVQKE
                     NTIIVEGAGDPEKIKARVASIRSQIEETTSEFDKEKLQERLAKLAGGVAVIQVGAATE
                     VEMKEKKLRIEDALAATRAAVEEGIVSGGGTAFVNALPKVKEVVRSLSGDEKTGAMIV
                     AKALEEPIKQIAINAGLEGAVIISKVMASKKGVGYNVVTEKYEDMIEAGIVDPTKVTR
                     SALQNAASVAALILTTESLVCDIPEKEAPMPAGNPGMGGYGM"
BASE COUNT         2974 a         2026 c         1504 g         3533 t
ORIGIN      
        1 gaccggtttt tcgacagctt ctaaatctta gcacagcgtt tttactgtgt caacagcaat
       61 ttcgctattt attacaaaaa atcaaagtaa tttttaacct tgtcagaaca agctcatttt
      121 tgcatgatat caattgtcaa atttaggaaa atctgcattg tttcgtacta atgtttatat
      181 attagattat ttttattata cttcatataa tcatttttta tcttcttcaa atcttattcc
      241 gttatttttt aaattctttt ctgacttatg aatgattatt atttaataaa cctgtaataa
      301 tgtatatata tcgtaatatt taaatactgt atcaatatta caataaaatc ttcttgaaat
      361 taaagtaatt taatattttc tatacttctg ctataaaagt taattgtatc tgttagtgtc
      421 ttctttggta taatgtattt taaattaact gctaagctgt ttgcttttaa tttgaatatt
      481 aataaaaaga ggccattatg tattattcta aaatgctcat accgacaatc agagaaaatc
      541 cccaggaagc agaagctata tctcataagc ttatgttaaa agccgggctt ataagacgac
      601 tggcttcagg aatatatact tttttaccgc ttggttacaa ggcattaaga aatgttgaaa
      661 gaataatcag acaagaaatg gaaagcaaag gagcgttaga actttttatg tctgccctta
      721 taccaaaaga attattggaa gaatcaggca gatgggaagt attcggacca gaaatgtttc
      781 gtctgaaaaa tcgtgaagaa agagatttct gtttgggccc tacccatgaa gagtccttca
      841 ctgtaacagc aagaaacgaa attaaatctg ctaagaatct tcctcttacg ctttatcaga
      901 tacagacaaa attccgcgat gaattacgtc cccgtttcgg aataatccgt tgcagagaat
      961 ttatgatgaa agatgcatat agttttgata aagatgagtt aggactggat atatcatatt
     1021 ctgcaatgta tgatgcatat acaaagatat ttaaacgctg cggattagat atagttatag
     1081 ctgatgctga ttcaggcgct atgggtggtt caggttctca ggaattcatg gttgtaaatg
     1141 atattggaga agatgaaata gtggtatgca gctcatgcgg ttatgctgct aatattgaaa
     1201 aagctgaagc agtgacaata aaaaggaatg tcaatgaaac acctttggaa aaagaactga
     1261 tacatactcc agatgctaaa acaatagatg accttaccag tttattctcc tgttcatcgc
     1321 agcagtttgc aaaatcactt gtgcttattg ctgattcaga acctgtcatg gctgtactaa
     1381 gaggagacag ggagctgtca cttaacaaat tggcttccat attaggttgt tccaacctgg
     1441 atatggcaga tgaattaacc attaaaaatc ttacaaaggc cgatgtaggc ttcgcatcac
     1501 ctgttggcat taaatgcaag gtaatatgcg ataatgaatt aatgaacatg gttaacttta
     1561 taactggagc caataagact gattatcatt ataaaaatgt taatctttca gatttcagtt
     1621 catatgagtt tcatgatata cgattggtta cagataatga tttgtgtcct aggtgtaatg
     1681 aaccattaaa aattgtaaaa accattgaag taggtcatat atttaagctt ggtgataaat
     1741 attcaaaagc tttagactgt aattatactg atgaatcagg cgaaagaaaa cccatgatca
     1801 tgggttgtta cggtattggt ctgggaagaa cccttgcagc tgttatagaa cagaataatg
     1861 atgaacatgg aataatctgg ccaatggaag tcgcgccata taaagtatat attcttccta
     1921 ctctgacaga tgacccgtta atatctgaaa tggcaaaaaa gatgcatgac gagcttgtca
     1981 gtaatggcat tgatgttatt ttagatgaca gaaatgaaag gccaggagtc aaatttaatg
     2041 atgcggacct tcttggaata ccattaagag ttactattgg aagaaatgca aaagacggcc
     2101 ttgtagaatt taaaaagaga agcgagaaag aggtttcttt aattacttat tcaaaagcta
     2161 ttgaagaaat tctgtcaann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnct
     2281 taataagatt tcctgttttt tagcaggtct gttcttcatt cttcctgtct tagcatttat
     2341 gctgtagaga cgatacttaa ttacattagt gacaaataaa tcctctctgt tcaaacctaa
     2401 agagttcata aacagcttaa gttttcctcc cgccagccct acaaaaggtt ttctttgaat
     2461 aacttcgtct tttcctggag cttcccctat aagcattaca cctttattca tctgaccatc
     2521 agaaaaaaca aactgaaagt tatactgact atctgttttt gataatactt ctgtgtattc
     2581 tttgtatatt aggtcaagtt tatcctttaa gtcttttcca agcactttta atatctctcc
     2641 acatatctat ctttttagat tcatccctta acagagcggc aggatgatat gtcattataa
     2701 ccggtatacc tttatacaca tcccatgttc ctctttgctg ggttatcctt atattcttac
     2761 gaataaggct ggaagccgct gtattgccaa gaagaattat tattttaggt tttacaagat
     2821 caatctgtct atcaagataa ggcatacagc tttctatttc ctcttcatat ggagtcctgt
     2881 tattctgagg tctgcattta atcacattac agatatagta atccttttct tcaatagaca
     2941 aagatgacaa tgccatagaa agcagttgtc ccgctttgcc tacaaatgct cttccctgtt
     3001 tatcctcttc ttctccggga gcttcgccta caagcataag gtcagctgtc ttactgcctc
     3061 tgcctattac aacattcttc ctattctccc acaatgagca tcttttacat tctgcgcatt
     3121 gcttcgttag ttcctgccat tcacttgtta atatatcgtt tgtcatggta ttaattatac
     3181 agtaataata tcctgttttt aagaccttcc ttatttttct tttttagtgt tttttatctc
     3241 tcgtgattat aaactacatc tgcaattgaa taaccgagaa ttccatctta atatcgtaac
     3301 tattatctca atttatcact ctttagattc ataaccgagc tgtcttgcta tttgctcaat
     3361 ctttgccagc cttttttgaa ctgtagactt tgacactttc acactcatat gctctgctat
     3421 ctcattgtat tcagtcagat cctcattcag ccgtagcatc gctgcttctt tcagcttacc
     3481 attcaaaaca tcaaatttct tgtttttttg aatgtacttt atagcattta tctgccttat
     3541 cttagtctca accgatttat ctgaattata ttcatcacaa ttcagcattc tgtttgtatc
     3601 attccttaag cttttaacaa ttcttttatt ctcataatca taaagagcat tgtgtgcacc
     3661 tatcatgttc aaaaaggttg aaatatcttc agaattatta aaataaacat attgcgtttt
     3721 acgtcttttt gatcttttag cattaactaa agattctttt attagcatat tatacagcct
     3781 ttcaatctca taactgtttt tggatgctat ctcaagataa tatccttttt cgggatcttt
     3841 gattgttcca gaagatagaa aagctccttc ataataggag tatctgcaac acgttgattc
     3901 agttaattcc gcaatatcag agaatattcc attattttgt tcatccactt ccattagacc
     3961 taaatcggaa tagacaacag aagctgcata tgtcgcttca gtcttaacac agaaaatcgt
     4021 attatcatta agcttgttct tcttcatatg tatatcagaa tgaagagaat aaatcctgtt
     4081 catagcaaat atgaaccacc ttgcttcagt attatcctgt gtatttatat ttaatgaata
     4141 atatccattt ctttctttta tgaaacggcc ttcaagacga acaatagcgc tgagtaatgc
     4201 tttctcacag cagtttttgg ttacatcctt atatgataat tcctttttta caatagtagt
     4261 gtaagacatt accttttgcc atattcttta acaatagcat ccttttcaat atctctgtgc
     4321 tgtattgtaa ccctgtgttt attcttttca agcctttttg caaattcatc agctatcaca
     4381 acagaacgat gtcgtcctcc ggtacaccct aacccgatta ccagggaagt ctttccttct
     4441 tttatataca aaggtatcag aaattctatc atttctgtaa gcattttcaa aaaatcctgc
     4501 gtagctttct gtccaagcac atattcaata acaggctttt cctttcccgt catttccttc
     4561 atttcatcaa tatagtatgg gtttggagta aaacgaagat caaatatcaa atcacagtca
     4621 accggattgc catacttcac gccatatgac actaaagtta ttatgagagt ttcttttagt
     4681 ggtccgccta gtactatttc tgcaagctcc tgcttgaatt cttttgttaa caactggctg
     4741 gtatcaataa catggtcagc tcttttactt atctctccga attcttctct ttccatttgt
     4801 atggcctgct gagtatttaa agaatcattt tccatcggat gatgtcttct ggtctctctg
     4861 tatcttttta ataacatcac atcagaagct acaagatata ttaatttata tacataaccc
     4921 tgttcatcta gggtatttag agcagaaagt atattttcca aaggttcggc tcctcttata
     4981 tcagcaacca gagctacctt ttctattttc ttgttatctg ctaccatttt cacaaattga
     5041 ggaataaaag aaggcggaat attatcaata caatagaacc ctaaatcttc cattgttttc
     5101 aatgcataag acttgccggc tccgctcatt ccagtgatta taaacaaatc cattattctt
     5161 ctccgatgat tctgacctcc ggttcgaggt ctactccata tttattatac acaaccgttt
     5221 ttacggtgtc cataacagtt aaaatatctt ttgatgttgc attaccggtg tttattatga
     5281 agccggcatg ctttgttgat acagatgccc cattaactgt atatcctttt aatcctgcat
     5341 cttcgataag tttgcctgca aaatgaccta atggcctttt aaatacgctt ccacaagacg
     5401 catactccaa aggctggctg gattttcttc ttcttgcata ttcaatcata tcttttttta
     5461 tactttcttt atccccttta ttaaaactga actttgcttc aagaattata aaaggcttgt
     5521 cagtataaaa acttcttctg cagccgaatt catgttcttt aagggacaga gtatgaatat
     5581 tcaaatcctc atccagataa gttgatgaaa tgactatgtc ttccatctgt gtaccgtaag
     5641 aaccggcatt catatataca gctccgccta cagaacctgg tatacctgat atagcttcag
     5701 caccggttaa tgaattctca taagcaaaac ctgccagttt agccatagat atacctgatt
     5761 gacatgtaac aatattttct tcagctttaa taccgttaag tttctgagtt aatatgacag
     5821 ctcctcttat tcctttatca gtaacaagga tattagtaca attgccaaat atgtatgcag
     5881 gcatgtttaa acttcttaag tcttttaaaa gagatatgag ctggtctgta ttttccacat
     5941 gtgcaagaat atctgcagaa ccacctgttt tataggcggt gtaattactc ataggctcat
     6001 tgtatctgat gaaatcatat tttttaagga gtgcttccaa atttcttgtt aaccctttca
     6061 tacatttctt ttgcagcgtt atatcccatt tttttctgtc tgaaatccat tgctgctact
     6121 tccattaata tcggcatatt tcgtccaggc attataggca gtgttaaaga tggtatcttt
     6181 attcctaaat attccgtata aacctcatca acaccaatac ggtcgtattt tttattatca
     6241 tcccatcttt caatatttat aaccagatct atactatcaa tgtccttaac agaaccggct
     6301 ccatataatc cttttacgtc aattatacca acacctctta attccatgaa atgacgaata
     6361 atttctggag ctgtccctaa aagagtagtc tgactgactt ttcttatctc cactacatca
     6421 tcagctatta gtctgtgtcc tctttttaca agttctagag ctgtttctga ttttcccaca
     6481 ccgctatggc cggttataag gatacctaca ccatacagtt ccaaaagttc gccatgtata
     6541 gtgatccttt cagcaagctc taagttgaga tagcttataa gcattgctaa aaatactgat
     6601 gtagtctcaa aagtcctcaa aaccggtact cttttaatcc ttgctgcttt aactatctca
     6661 tcaaatacag gcaaagaatt ggatattata atacatggta tatcttcatg catataatct
     6721 tccagttttt tcagtctttc agttctggaa agagtaccaa gaaaattcat ctccgttctt
     6781 ccaaacaact gaactctttc aggtttaaat ccttcaagat aacctgcaag ctgtaaccct
     6841 ggacgataaa cttcaataac gcttattgtc ctatcgtcga tattttcagg aatatatact
     6901 ttatccagct gatatctttc aattattctg ttaacgctta tactatgtat tgaatcatcg
     6961 taatcatctg acaaatccat acagtacctc ctgtaataga gaattagtag ctaatatatc
     7021 acttttatgt ttcattgatg gaagctcgcc tttccagtca gtacatatac ctcctgcttc
     7081 ctttaaaatg cagcaggcag ccgcataatc ccatggtttg agattgcttg ttaaaaaggc
     7141 atcagtcttc ccttctgcaa cataacatat atcnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7261 nnnnnnnnnn nnncagtctg ttacaaaatc cctttctcct tttttagtaa gattacccct
     7321 attaacttta tcaaggacta tcttaccagc atctataacc gcctttgtga cagttgaaaa
     7381 atccattaca ggcctctttt atattcaact ccgtcaataa tggcagatgt tccgtcacgt
     7441 tcataggaat aagtaacttc cgtatcatca tacttaaatg taatactatc ttttttaata
     7501 gagtatgttc cggttatttc actatcccct tcagttaaaa taaactcatt gttcatgaat
     7561 tcatattttc tgccgcttag ctcatcttca tactcaccga aaagataagt ctgacatgca
     7621 ctaagagtaa gcaataacgc aattattaat ataaggactg ataatctttt catatgcaat
     7681 tatgcacctc cacgttttct cactagatta tatcatgtct tggatatttg ctctacctgc
     7741 tgaggctcct ttaataatgt ttttacatta ggcatatata catcatgtgt aaccagattc
     7801 ttaattttcc cttttctttc acaattttta aatgtcttaa aacctataaa agtttcaact
     7861 ggttcacaat tatcatcaga agatactatt acattcttta aaaataaatt ttcaacacga
     7921 tcatatatct gaatatattc ttcaaccttt gaatcttttt ctgactgaag aattgtaaga
     7981 ccgtcaataa ccgcattttt tatgcatggc ctgaaatcat cagcataatt caaatcattc
     8041 ctatggaaag gcaatccgaa aataaacaaa ggcctgtcat ctgatgggtc ctgatgatgt
     8101 atgttcttaa aagtaatgca gtcaaagttt cccccaatat caaacagtgt tgctgtcctg
     8161 tatggataaa catggtctaa aggccttaaa tctatatttt caaaatatat gtgcccgtaa
     8221 tttccaaagc cgtctcctgg aaaccaggga tttataaaaa atccataact tctgtaggta
     8281 cctgttacat tcttaactgt aactctgtcc agtcttcctt ttgctcttga aagcattcga
     8341 atagcctggt ctgcattttc taaatgaact ccgtctataa gtacatcagt aatggaagaa
     8401 accaggtcat gttcatcagg acctagggca ataaaatcgt ctccagctct tcctttaata
     8461 ttccttaaag tcagaaactg ccccgggccc cagaaatgca gtccatcctg gttctgccct
     8521 ttcatccagt tgggaagatc tatatatatg ttttccatag ttacattttt aaaattcacg
     8581 cacataaccg accatgctct ttggtttctt atcgttatat cccttacaac aagatcttct
     8641 atcccaaaga attcaaaagc gacaaccagc ctgcttgcgc ccatatcatc agatattcct
     8701 tctataccga aacggtattt ctcatctgtt atgggtacat ggtgctgctg ccctttatta
     8761 ttatggttat acgtcccccc gataagtgtt acattccttg ttaacctttc agtatagctt
     8821 ctttccttta aatctattaa tgaacggtca gaaccatctt tcagaaaaaa accgcagtct
     8881 ttgctcagac actctattgt ggtatttgac tttacatcaa gtccggttat aaggccggct
     8941 ccatccataa tcagatgaac tccattattg ttatctgctt tatctaaaat cttttgcaga
     9001 gcacttgtaa catcagtacc tccacctgaa tatatattac tgtccaatgt agctatctca
     9061 cttgctaaaa atgtttgaaa tttcatattc gatttatatg atacagtcag tactgctcac
     9121 tttcctcctt cacagatagc attattttaa catcatatta gagtattaca acaaaatata
     9181 gtcagttctg gttatgtgaa caataatata taaatacaga aaacaaaaga ctgccaattg
     9241 gcagtccttg ttataatata atttattaaa attccttatt acattccgta tccgcccatt
     9301 cctgggtttc ctgctggcat tggagcttct ttttctggaa tatcacaaac aagtgattct
     9361 gttgtaagga tgagtgctgc aactgatgct gcattctgaa gagcgcttct ggtaacttta
     9421 gtaggatcta ctataccagc ttctatcata tcttcatatt tctcagttac tacattgtag
     9481 ccaacaccct ttttagatgc cattacttta gaaataatga ctgcaccttc aaggcctgca
     9541 ttaatagcta tctgcttgat aggttcttct aatgcttttg ctactatcat agcaccagtc
     9601 ttctcgtcac ctgataaact gcgtacaact tctttaactt ttggaagtgc attaacaaat
     9661 gctgtaccgc caccggaaac tataccttct tcaaccgctg ctcttgttgc tgcaagtgcg
     9721 tcttcaattc ttaatttctt ttctttcatt tcaacttcag tagctgcacc aacctgaatt
     9781 actgcaactc cacctgcaag tttagcaagt ctttcctgaa gtttttcctt atcaaattca
     9841 gaagtagttt cttctatctg tgatctgatt gaagcaactc ttgctttaat cttttcagga
     9901 tctccagcac cttcaactat aatagtgttt tctttctgta ctttaacctg tcttgctctt
     9961 ccaagcatat caactttagt ttccttaagg tctaatccga gttcttcaga aattacctgt
    10021 ccgcctgtga gaatagctat atcctcaagc attgcttttc ttctgtcgcc gaatccagga
    10081 gctttaactg ctacacagtt gaatgttcct cttagtttat taactactaa agtagctaat
    10141 gcttcgcctt caacatcttc tgctacgatg ataagcttct tgcctgactg aactatctgc
    10201 tctaaaagag gaagaatctc ttgtatattt gatatct
//
LOCUS       DAOSXM010000072        10220 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2111
            NODE_4149_length_10220_cov_11.358485, whole genome shotgun
            sequence.
ACCESSION   DAOSXM010000072 DAOSXM010000000
VERSION     DAOSXM010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864178
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 10220)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10220)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:24:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,261
            CDSs (total)                      :: 2,229
            Genes (coding)                    :: 2,218
            CDSs (with protein)               :: 2,218
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 3 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10220
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4149_length_10220_cov_11.358485"
                     /isolate="AMR_MDS_2111"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            119..655
                     /locus_tag="PLQ68_08650"
     CDS             119..655
                     /locus_tag="PLQ68_08650"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXK72760.1"
                     /translation="MERAASVFLHADIDKTKATKLLAWIKDPIVTRYLNEKSDVAEKL
                     NEVIQNTHPSLLACHLNKSGRFFFVCEKNNEPIGFARLADTDNPNEYELVIVIGRKDM
                     WGQGYGTKAVTQCLNTAFFEWRADRVSAYVHMDNIGCAKVLEKSGMKQVSQSENCDYF
                     NVTFDEYLSRNTKKEHTL"
     gene            848..2047
                     /locus_tag="PLQ68_08655"
     CDS             848..2047
                     /locus_tag="PLQ68_08655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72761.1"
                     /translation="MNLYSGFKTEKDPETGRRLIRLTEWDAFCYPLYYFIPSVTDDER
                     FLVYHRCNETEVQLWVLNLQTGEHRQLTHASNNANDAQWRPWCSPCKNGVLDHRSVLN
                     TVNNNVIFFDQNIAIMINIETLEQKNLFTLPQHRYPISQNCVTSDGKWFVFSHMDKAF
                     YYEMTAGNWSDYIKYRPLTKDCTIQAYNLETSEMRDILILNAVMHHIIPYGNEYFAIN
                     HPPTESGILFTGLEGRWYTHIRTKSDDGRVANHCAATKRGIVYEASKFHGDNCGGIYH
                     PITHRFIEYKLPEFFGYTHTGYDPNGNLIFYENLDYKDTTINCHNMYKMNRYEPDGPC
                     SWELLMGNRQTFGSRSSQKSHFHPRITPDNRYILFTGGYEKTHTNQLYLLDISDVRPT
                     ENLPDLS"
     gene            complement(2145..3407)
                     /locus_tag="PLQ68_08660"
     CDS             complement(2145..3407)
                     /locus_tag="PLQ68_08660"
                     /inference="COORDINATES: protein motif:HMM:NF014600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HXK72762.1"
                     /translation="MKKNVTTIIAICFIAAIVIAVVIFLVQQKNNDVENPTPTVTPSI
                     SHSQTNEPTQSQNITEVPLEGLNEKLADVNNPITITLNSEQLKIIDASVSSETYALVY
                     FPDRNISNYENGQFILKTSVFHETNNYIGTITIVSDSYVKAGYLLADKDGKIYIENSE
                     KNQISADVSLRIINRYNPITQQQADDIEGLVNLTNPWYVLDERDDLYILSHVKTAMES
                     LLTEADRQDSSLNMHVASGYRDYASQKQSFDYWVNRRVTQENMTYEQAYDYTAGRVAI
                     PGCSEHHNGLTVDLISYGYMLDESSKDAPYAKWLKDNSYKFGFTIRYEEGKEQYTHIT
                     LYEPWHIRYVGLPTAYYLYANNFCMEEFYETLINEGYLNFEYEGETYRYIYSKTDNIY
                     INQNIMDIVQYSSIASGVEGYVLLCKIR"
     gene            complement(3412..4278)
                     /gene="hslO"
                     /locus_tag="PLQ68_08665"
     CDS             complement(3412..4278)
                     /gene="hslO"
                     /locus_tag="PLQ68_08665"
                     /inference="COORDINATES: protein motif:HMM:NF001033.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HXK72763.1"
                     /translation="MDSILKAMAYDKKVRVYAALSTDTVNQAIKKHNLNLLSAMALGR
                     LLTATAMMSYMTKSESLIVSISTDAALKGLTAISEDKGLVKGYVHNNDYTTYGEDISS
                     VGQLLMPGKLMISRDMGLKEPYTGIISLVTGEIAEDIAKYFTDSEQTPTACALGTYFD
                     EKGRLLASGGYIIQVMPGTDDSILKELEQRVSLTENISKLIYDLKTPKNLLNFLFSDR
                     NLEVTGTEKMAFYCGCSKDKIMEKLLTIDKKQLEQVAKEQSEIEAVCPYCNNHYLYNS
                     DDILKLLKDIES"
     gene            complement(4282..5019)
                     /locus_tag="PLQ68_08670"
     CDS             complement(4282..5019)
                     /locus_tag="PLQ68_08670"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HXK72764.1"
                     /translation="MYDCIAGVYDLLMRDVDYVSYAGYIDGILKRYLNNKPDIVLDVG
                     CGTGSLTCAMRDLGYSMIGAEPSERMLECALKKDTNNIQYINQSMEKLDLFGTVGAAI
                     SFLDCINHITDIKKLQQGFKKVSLFLEPEGLYIFDLNTEYKFENIYAGNLYYDLSDDV
                     SYVWVNNYSKKTKICTMDISYFIKNDDGKYKRYDTCNRERAYSDKQIIELANNSGLEL
                     IDMLEDFSFDKPRRNCQRKFLIFRKKR"
     gene            complement(5019..6290)
                     /locus_tag="PLQ68_08675"
     CDS             complement(5019..6290)
                     /locus_tag="PLQ68_08675"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HXK72765.1"
                     /translation="MKKLLVKKTIAILVIMAITSFALLTGCSFIADEILDIDKAIARR
                     ITTVTMPEVSPSNIQLEGDFTEEQLEKFNEVYNLLKQTSIYELDHDEIMESIIAGMAS
                     AFDVYTMYVPEEYANTIRESASGEYKGIGVTITTPEDGIGAEVISVNPLGDAYVQGIK
                     TGDIIIRVEETELTKSMSLEYIASIVKGEEGTKVNVTVWRPSEQKEIQFSITRMLINS
                     IDASGHLIEGTDIGYIAISSFSADMLDEFVNTFNDLVLVDGMTKLIIDLRDNPGGDFY
                     TAVYLADAFIKDGIITTLADGTGYKEEYTADSYAISIPTVILVNGNSASASELFSGAM
                     KHYGLATIVGTQTYGKGVAQSIYDLDDGSQLRITSYQYLLPDGSNIDGIGVTPDIEVL
                     PFEGFENEYISSLEPENDAQLQKAIEILENQ"
     gene            complement(6327..7487)
                     /locus_tag="PLQ68_08680"
     CDS             complement(6327..7487)
                     /locus_tag="PLQ68_08680"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HXK72766.1"
                     /translation="MIKGTYKKRILTMILALIMLFSFTQAAFCVADINEAKDNLGTIQ
                     EELESVENELKQKQSIKDYYESLTKNMKNSIDSQQLQLATYNEYIVEVTNKINSLTDT
                     IEQAEEEYEQKISVLKKRIVETYISSDLTMFEVLMKSSSLKQYYDYIELNSLIAKHDQ
                     ELIDEMKILMQDISDKRETMKMLKASYVTLSEQAQIVLSNMQNMQQTAIETVNITEKT
                     IEVLKAKENALAAEEAEMLNVILRLQREIDYIGGQMTWPLPSCKILPTGGSLFGWRLH
                     PIFFEWRMHNGVDLGGKQGNNIVSTNAGVISIASWGEGYGYYVVVDHGGGVSTLYAHC
                     SKILVSAGQSVKKGQVIALIGSTGWSTAPHLHFEVIISGKRVDPLLYIDPSR"
     gene            complement(7501..8385)
                     /locus_tag="PLQ68_08685"
     CDS             complement(7501..8385)
                     /locus_tag="PLQ68_08685"
                     /inference="COORDINATES: protein motif:HMM:NF037183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HXK72767.1"
                     /translation="MSIKYILVESFNNILRNKVLFITSIFTVCVTILLMGVVGILLMN
                     VSYNSDEMLDEMLHITVYVIPEADEKTLNDIQDVIVSDSHVESSTYVSKLEAYEASKE
                     LVGEEVMEELGYDFLPPSFIVKLNDVTYANIFVHKMSSMSNIYEVKYSSESYERAGRI
                     SYTIRYVCGALTGLLGVLSLFLISNTIKITVSSSKEEVTIMRYIGASEARIKVPFFLQ
                     GGFIGFFGAIISFIIAGFGYNYIYTKLEGSLSEYIGVLELVPIPQMLLVLLGVFMLYG
                     IVLGVLGSFFAIRKYLKA"
     gene            complement(8375..9070)
                     /gene="ftsE"
                     /locus_tag="PLQ68_08690"
     CDS             complement(8375..9070)
                     /gene="ftsE"
                     /locus_tag="PLQ68_08690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014253850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HXK72768.1"
                     /translation="MIDFENVSMIYRSGTEALHGISFHVDPGEFVFITGKSGSGKSTI
                     IKLLMREEKPTAGDIFVNGYDLYNLSRYDLPHYRRRLGVVFQDCRLLMNKTVYQNIEF
                     AMEVVGESNRTIKQVVPKLLHMVDLVGKEKAFPDELAGGEQARVALARAIANNPDILI
                     ADEPTGNLDPKTSTEIIDLLYKVNQLGTTVLVVTHDTKIVDRLQQRVIRLKAGNIISD
                     TEGSIYEDETCEY"
     gene            complement(9072..10127)
                     /locus_tag="PLQ68_08695"
     CDS             complement(9072..10127)
                     /locus_tag="PLQ68_08695"
                     /inference="COORDINATES: protein motif:HMM:NF037653.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HXK72769.1"
                     /translation="MLKELREQLNEQLGCLFGIVGMKGTILLCSDTSQEGKEFVRFDE
                     LAKNTKEKIAIEGLVFTKIKAVKNAQYFVFVEDLGMNTDVILNLVALYAKDYLRSNTE
                     KTDKEFFLKQILQEDLSKEEIDKRNKIFKLNVTDKKAIINIRTQKIPEYPVSDILKNL
                     FHDLKDANIVTLDNNDTVVILPLRDDLNNVLQSVISTLEEHLMVSVKASIGKYFYLLE
                     EAKYAYISSVKTFDIGSLYKEGCSIYDYNNMGTARLFHDISVERLESFIEETITSEVY
                     DQIDDETIKTMQAFFENNLNISETARKMYLHRNTLVYRIEKFNKLTGLDVTKFESAVL
                     FYTICQIKCYLRNLGRD"
BASE COUNT         3116 a         2026 c         1553 g         3525 t
ORIGIN      
        1 aaaacagcaa ttgatgttga attaaactta tttacatgtt accattaaaa tacaaaaaga
       61 accgtcgttg tttatcaagg aagggctttt aaatataatt tactgcgagg taagagatat
      121 ggaaagagcc gcttctgttt ttttacatgc ggacatcgat aagaccaaag caactaagct
      181 tttggcatgg ataaaggatc caatagtaac cagatatctg aacgaaaagt ctgatgtagc
      241 agaaaagctc aatgaagtaa tacagaatac tcatccgtca cttcttgctt gtcatttgaa
      301 caagagtgga agattctttt ttgtatgtga aaaaaacaat gagcctatcg gatttgctag
      361 acttgctgat acagacaacc ctaacgaata tgaacttgtt attgtaattg gcagaaaaga
      421 tatgtggggg cagggatacg gaacaaaagc tgtaactcag tgcctgaata cagcattttt
      481 cgagtggaga gctgacagag tatctgctta tgtacatatg gataacattg gatgtgcaaa
      541 agtattggag aaaagtggaa tgaaacaagt ttcacaaagt gagaactgtg attattttaa
      601 cgtcactttt gatgagtatc tatcccgcaa tacaaaaaag gaacatacat tataggtata
      661 aaatggctaa gttatctcaa atagcagctt attaaattgc ctgaaaaagt gttgacttct
      721 tttaatttag cgatataaga ttgtattgta attatagcca aagtgaaaga aatattattt
      781 tcgatgcaca aaacatacct ctgtacttcg gacacaatag acaatactga aagagatgga
      841 tttgaaaatg aatctgtata gcggttttaa aacagaaaaa gaccctgaaa caggaagacg
      901 gcttatacgc ctgactgagt gggatgcatt ttgttatcct ttatattatt tcataccctc
      961 agttacagat gatgagcgtt ttttagttta ccatagatgt aatgagacag aagttcagtt
     1021 gtgggtactg aatcttcaga caggtgaaca tcggcaactg actcatgcca gcaacaacgc
     1081 aaatgatgca caatggcgac cttggtgctc tccttgcaaa aatggcgtac tggatcatcg
     1141 aagcgtatta aatactgtca ataacaatgt aatatttttc gaccaaaata ttgctatcat
     1201 gattaacatt gaaacactgg agcaaaaaaa cctgttcaca ctgccacagc acagataccc
     1261 tatatcgcaa aactgcgtaa catctgatgg taaatggttt gttttttctc acatggacaa
     1321 ggctttctat tatgagatga ccgcaggaaa ctggtcggac tatataaaat atcgtccatt
     1381 aaccaaagat tgtacaatac aggcatataa tctagagacc tcagaaatgc gagatattct
     1441 tattttaaat gcagtaatgc atcatattat tccatatgga aatgaatatt ttgccataaa
     1501 tcatccacct actgaatcag gcatactttt taccggcttg gaaggacgct ggtatacgca
     1561 catccgcact aagtcggatg atgggcgtgt tgcaaatcac tgcgccgcta ctaaacgagg
     1621 catcgtatat gaagcaagca aattccacgg ggataactgt ggaggcatct accatcctat
     1681 tacccatcgc ttcattgagt acaagcttcc tgaatttttc ggttacactc acacaggtta
     1741 cgacccgaat gggaatttaa ttttttatga aaatctggat tacaaagata ccaccattaa
     1801 ctgccacaac atgtataaaa tgaaccgata tgaacctgat ggtccctgca gctgggaact
     1861 tcttatggga aacagacaga catttggttc acggtcctct cagaaatcac attttcatcc
     1921 aaggatcaca cctgacaacc gctatatact cttcactggc gggtatgaga aaacacatac
     1981 taatcagttg tatctacttg atatttctga cgtcagacct accgagaatt tacctgattt
     2041 aagttgaagt aataatttaa aggaagaata tatctgcttt tattatagat acataatgag
     2101 attttacttt tttgatttat attctcttat atattctctt ttatctatct tatcttacat
     2161 aacagtacat aaccttccac acctgacgct atagacgagt actgtacaat atccattatg
     2221 ttttgattta tgtatatatt atcggtttta gaatatatat atctgtatgt ttctccttca
     2281 tattcgaaat ttagataccc ctcatttata agggtttcat aaaattcttc catacaaaaa
     2341 ttatttgcat aaagataata tgctgtaggt aatccaacat atctaatgtg ccacggctca
     2401 tacaaagtta tatgagtgta ctgttctttg ccctcttcat acctaatagt aaatccgaat
     2461 ttatagctgt tgtcttttag ccatttagca tatggcgcgt cttttgaaga ttcatcaagc
     2521 atgtatccat aacttatcaa atcaactgtt aatccgttat gatgctcaga acatcctggt
     2581 attgccacgc gtcccgctgt ataatcatac gcctgttcat atgtcatatt ctcctgtgta
     2641 actcttctat taacccagta gtcaaaactc tgcttttgtg aagcataatc gcgataaccg
     2701 cttgctacat gcatattcaa agatgaatcc tgtcggtcag cttcagtaag taagctttcc
     2761 atagctgttt tgacatgact aagaatatat aagtcatcac gttcatcaag aacataccaa
     2821 gggtttgtga gatttacaag accctctata tcatctgcct gctgttgtgt tattggattg
     2881 tatctgttta ttatcctcaa tgaaacatca gctgaaatct gatttttttc actattttca
     2941 atgtatattt ttccatcctt atctgctaaa agataccccg cttttacata tgaatcagat
     3001 actattgtaa tagtaccgat ataattgttt gtttcatgaa aaactgaagt ttttaaaata
     3061 aactgtccat tctcataatt tgatatattt ctatctggaa agtatacaag cgcatatgtt
     3121 tcactgctga cacttgcatc gattatctta agctgttcag aatttagtgt tatggtgatt
     3181 ggattattta catccgcaag cttctcattt agcccttcaa gaggaacttc agtaatattc
     3241 tgtgactgtg tcggttcatt tgtctgcgaa tgagaaatgg aaggagtaac tgtcggtgtt
     3301 gggttttcaa catcattatt cttctgctga actagaaaga taacaacagc aataacaata
     3361 gctgctataa aacatattgc aataatggtg gtaacattct ttttcatgat atcagctttc
     3421 tatatcctta agcaatttca aaatatcgtc actgttatat aaataatggt tattgcaata
     3481 tggacatact gcttctattt cagactgttc ttttgccacc tgttccagtt gtttcttatc
     3541 tattgttaac agtttttcca ttatcttatc tttactgcaa ccacagtaaa aagccatttt
     3601 ctcagttcct gtaacttcaa gatttctgtc tgaaaaaaga aagttcagca gattttttgg
     3661 agttttaagg tcgtatatga gcttggaaat gttttcggtg agagataccc tctgttctaa
     3721 ctcttttaaa attgaatcat cggtaccagg cataacctga ataatgtaac cgcctgatgc
     3781 aagtaatcta cccttttcat caaaatatgt tcccaatgca caagctgttg gcgtttgttc
     3841 gctgtcagta aagtatttag ctatatcttc tgcaatttca cctgtaacca aagatatgat
     3901 accggtatat ggctctttta atcccatatc tcttgatatc ataagcttgc cgggcattaa
     3961 aagctgtcca actgatgata tatcctcacc atatgtagtg taatcattgt tatgaacgta
     4021 accctttacc aaacctttgt cttctgagat agctgtcaaa cctttcaaag ctgcatctgt
     4081 agatatactg acaatcagac tttcactttt agtcatatat gacatcatgg cagtagctgt
     4141 taaaagtctt cctaatgcca tagcagaaag cagattaaga ttatgctttt taattgcctg
     4201 attaactgta tctgtactta gtgcagcata tacccttact tttttatcat aagccattgc
     4261 ctttaatata ctatccataa atcacctttt ttttctaaat attaaaaatt tcctctgaca
     4321 gtttcttctg ggtttatcaa atgaaaaatc ttccagcata tcaatcagtt ccaatccgct
     4381 attattagcc agttcgataa tctgcttatc tgaatatgct ctttctctat tacatgtatc
     4441 atatcttttg tatttaccat catcgttttt tataaaatat gaaatgtcca tagtgcagat
     4501 tttcgtcttc ttagaataat tatttaccca gacatatgaa acatcatcag aaagatcata
     4561 atacagattg cctgcatata tattctcaaa tttatattca gtattcaagt caaaaatata
     4621 taatccttcc ggttctaaaa aaagcgatac ctttttaaat ccctgttgca acttttttat
     4681 atcagttata tggttaatgc agtctaaaaa acttatcgca gcgccaacag ttccgaagag
     4741 gtccagtttt tccatactct gatttatata ctgaatgttg ttagtatctt tttttagagc
     4801 gcactccagc attctttctg atggttcagc acctatcatg gaatatccca agtctctcat
     4861 tgcgcacgta aggctgcctg ttccgcagcc tacgtctagt actatatcag gtttattgtt
     4921 taaatacctc tttaaaattc cgtcaatata tcctgcatat gatacataat caacatctct
     4981 cataagcagg tcatatacac ctgctataca atcgtacatt attgattctc cagtatctca
     5041 atagcttttt gaagctgtgc atcgttttcc ggttctaaag aggaaatata ttcgttttca
     5101 aacccttcaa atggtaatac ttctatatct ggagtcactc cgattccatc aatattcgaa
     5161 ccgtcaggta ataaatactg gtaactggtt atcctaagtt gtgaaccgtc atctaaatca
     5221 taaatacttt gagctactcc tttgccatat gtctgcgtac caactattgt tgcaagtcca
     5281 taatgcttca ttgctccgct gaataattcc gaagcagaag cagaatttcc attcacaagt
     5341 attactgtag gtattgatat tgcataactg tctgctgtat attcttcctt ataccctgtt
     5401 ccatctgcaa gtgtggttat aattccatct tttataaatg catcagcaag atatactgca
     5461 gtatagaaat ctcctccagg gttgtctcta agatcaataa ttaacttagt cataccatct
     5521 actaaaacca aatcattaaa ggtattaaca aactcatcaa gcatatcggc actgaaacta
     5581 ctgatagcta tatatcctat atcagttcct tcaataagat gacctgatgc gtcaatggag
     5641 tttataagca ttcgggttat agaaaactgt atttcctttt gttcagaagg tctccataca
     5701 gtaacattta cttttgttcc ttcttctcct ttaacaattg aagctatata ttccaaagac
     5761 atgctttttg tcagttcagt ttcttccact cttataatta tatcgccagt ttttattccc
     5821 tggacataag catctcccaa aggattaact gatattacct ctgctccaat accatcttca
     5881 ggtgtagtta tagtaactcc gattccttta tattctcctg atgctgattc tcttattgta
     5941 ttagcatatt cttcaggtac atacattgta tagacatcga aagcagaagc cattccagca
     6001 ataatagatt ccatgatttc atcatggtca agctcatata ttgatgtttg tttcaaaaga
     6061 ttatacactt cattaaattt ttcaagctgt tcttcagtaa aatcaccttc taactgaata
     6121 ttgctaggag atacttcagg catggtaact gtagttattc tacgtgctat ggctttgtct
     6181 atatcaagta tttcatctgc tataaatgag catcctgtta ataaggcaaa tgatgttatt
     6241 gccataatta caagtatggc tattgttttt ttaactaata attttttcaa ttttaccacc
     6301 ttttcctata tccaaataag gattatttat cttgacggat caatatataa aagaggatct
     6361 actctctttc cgcttattat tacttcaaaa tgaagatgcg gtgcggttga ccagcctgtt
     6421 gatcctataa gagctattac ttgtcctttc ttgaccgact gtcctgcact tactaaaatc
     6481 tttgagcagt gggcataaag cgttgaaacg cctccgccat ggtcaacgac aacataatac
     6541 ccataacctt ctccccatga tgcgattgat ataactccag catttgtgga tacaatattg
     6601 ttaccctgtt taccgccaag atccactccg ttatgcattc tccattcaaa gaatatcgga
     6661 tgaagcctcc atccgaataa acttcctcct gttggaagaa ttttacaaga aggaagtggc
     6721 caggtcatct gcccgcctat atagtctatt tctctttgaa gccttaagat tacatttaac
     6781 atctccgctt cttcagcagc taaagcattc tctttcgctt ttagaacttc tatagttttt
     6841 tctgttatat ttacagtctc tattgctgtc tgttgcatat tctgcatatt acttagcaca
     6901 atctgtgcct gctcactaag ggtaacatat gaagctttaa gcatcttcat tgtttctctt
     6961 ttgtcactta tgtcctgcat aagtattttc atttcatcta taagttcctg gtcatgttta
     7021 gcaattaatg agttaagctc tatataatca taatattgct tgagtgaaga tgatttcatt
     7081 aatacttcaa acattgtaag atcactgctt atataagttt caacaattct cttttttaat
     7141 acagaaattt tttgctcata ttcctcttca gcttgttcaa ttgtatcagt cagactatta
     7201 atcttgttgg taacttctac aatatattca ttgtatgtag ccagttgaag ctgttgcgaa
     7261 tcaatactat ttttcatatt ctttgtcagg ctttcataat agtcttttat actctgcttt
     7321 tgcttcaatt cattctcaac actctcaagc tcttcctgta ttgtgccaag attatctttt
     7381 gcttcattaa tatcagctac acaaaatgct gcctgcgtga aagaaaacag cattattaaa
     7441 gccaaaatca ttgtcaatat tctcttcttg tacgtaccct ttatcatttg taaactcctc
     7501 ttatgctttc agatacttgc gtattgcgaa aaagcttccc aatactccaa gcactatgcc
     7561 atacaacata aatacgccta acaatacaag aagcatctgt ggaataggaa caagttccag
     7621 tactcctata tattcgctta ggctgccctc taactttgta tatatataat tatatccaaa
     7681 tccagcaata atgaaagata taattgcacc aaaaaagcct ataaatccac cttgcaagaa
     7741 gaaaggcacc ttaattctgg cttctgaagc tcctatatac ctcattattg ttacttcctc
     7801 tttactggaa gacactgtta tcttgattgt gtttgatatc agaaacagtg ataatacccc
     7861 taaaagccca gtaagagcac cacacacata cctgattgta tatgatattc ttcctgctct
     7921 ttcgtatgac tcagaactgt acttaacttc atatatgttt gacattgatg acatcttatg
     7981 aacaaaaata ttagcatatg ttacatcgtt cagcttaact atgaaagaag gcggaagaaa
     8041 atcatatccc aactcctcca tcacttcttc tcctactaat tctttgcttg cttcgtatgc
     8101 ctcaagtttg gatacatagg ttgagctttc aacatgagaa tcactaacaa taacatcctg
     8161 tatatcatta agggtctttt catctgcttc aggaattaca tatacggtaa tatgaagcat
     8221 ttcatcaagc atctcatcag aattgtatga tacattcatc aatagtatcc cgactactcc
     8281 cattaaaaga atagttacac aaacagtaaa tatgctggta ataaacaaaa ccttgtttct
     8341 taggatatta ttgaatgatt ctactaatat atatttaata ctcacaggtt tcatcttcgt
     8401 atatgcttcc ttccgtatca cttataatat tcccagcctt aagccgtatt accctttgct
     8461 gcagtctgtc aactattttt gtgtcatgtg taacaactaa aaccgtagtt cctagttggt
     8521 taactttata taaaaggtca ataatttctg tacttgtttt tggatcaaga ttgccagtgg
     8581 gttcatcagc tataaggata tcaggattat tagctatagc tcttgccaga gctacccttg
     8641 cctgttcccc tcctgcgagc tcgtctggaa aggccttttc cttgccaaca aggtccacca
     8701 tatgaagaag cttagggacg acttgtttta tagttctgtt tgattcgcca acaacttcca
     8761 tcgcaaactc aatgttctgg taaaccgttt tgttcatcaa aagtctgcaa tcttgaaata
     8821 ctaccccaag ccttcttctg taatgaggaa ggtcatatct cgataaattg tataaatcat
     8881 atccattaac aaagatatcc cctgcagtag gtttttcctc tcgcataagc aattttataa
     8941 tcgtggactt tcctgaaccc gattttcctg ttataaacac gaattcacct ggatcaacat
     9001 gaaaactaat cccatgcagg gcttcagttc cacttctata tatcattgat acattttcaa
     9061 aatctatcat actaatccct tcccaaattc cttaaatagc attttatctg acatatcgta
     9121 taaaacagca ctgctgattc aaatttcgta acatcaagcc ctgtaagctt gttgaacttt
     9181 tcaatacgat atactaatgt atttctatgt aaatacatct ttcgtgcagt ttcacttata
     9241 ttaagattat tttcaaaaaa tgcctgcatt gtctttattg tttcatcatc aatctgatca
     9301 taaacttctg aagtaatggt ttcttcaata aaactttcta gtctttcaac agatatatca
     9361 tgaaacagcc ttgctgtccc catattattg taatcataaa tagagcatcc ttctttgtat
     9421 agcgaaccta tatcaaatgt ttttacagat gaaatgtatg catactttgc ttcttctaaa
     9481 agatagaaat acttcccgat tgaagctttt acagaaacca tcagatgctc ttctaaagtt
     9541 gaaataacag attgcaatac attattaagg tcatctctta acggtaatat tacgacagtg
     9601 tcattgttat ccaaagtaac aatattggca tctttgagat cgtgaaacag gtttttcagt
     9661 atatcactta ccggatactc aggtattttc tgagttctta tattaattat tgctttctta
     9721 tctgtaacat tcagtttgaa aattttgttt cgtttgtcta tctcttcttt tgataaatcc
     9781 tcttgtagga tttgttttag aaagaactct ttatctgtct tttctgtatt ggaacgaaga
     9841 tagtcttttg catacaaagc aacaagattc aaaataacat ctgtattcat tcctaaatct
     9901 tctacgaata caaaatattg cgcattctta acagctttga tttttgtaaa aacaagccct
     9961 tcaatggcga ttttctcttt agtgttcttc gcaagctcgt cgaatcgaac aaattccttt
    10021 ccttcttggg aagtgtcgga acacaataat attgtcccct tcataccgac gattccaaaa
    10081 aggcacccta attgctcatt caactgctct ctcaattcct ttagcaagtc agatccgcct
    10141 ttcttaactt atattaacat aatatattat attatgtaaa cacattaatt atacagtaac
    10201 atcgactttt tgtatttgtt
//
LOCUS       DAOSXM010000073        10125 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2111
            NODE_4194_length_10125_cov_11.144290, whole genome shotgun
            sequence.
ACCESSION   DAOSXM010000073 DAOSXM010000000
VERSION     DAOSXM010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864178
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 10125)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10125)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:24:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,261
            CDSs (total)                      :: 2,229
            Genes (coding)                    :: 2,218
            CDSs (with protein)               :: 2,218
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 3 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10125
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4194_length_10125_cov_11.144290"
                     /isolate="AMR_MDS_2111"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..160)
                     /locus_tag="PLQ68_08700"
     CDS             complement(<1..160)
                     /locus_tag="PLQ68_08700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005814967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein Fic"
                     /protein_id="HXK72770.1"
                     /translation="MESGYITAQQAADKWNISDRRVRVLCSEGKISGVIKDGKSYKIP
                     FNAEKPADG"
     gene            complement(354..806)
                     /locus_tag="PLQ68_08705"
     CDS             complement(354..806)
                     /locus_tag="PLQ68_08705"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015393545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HXK72771.1"
                     /translation="MNIKIVKGTCEYIKDCEEALLKSSLGEQYFSSEKSALKAIEEAL
                     DKGNLYVAITDNICVGFFFYITNGAFHSYPYLHLIAVKEEYRGKGIGRQLVEFFENIS
                     GKDKFFLAVADYNEKAKRFYESIGYCQVGEIPGLYRKGITEYIMMKVK"
     gene            complement(995..1306)
                     /locus_tag="PLQ68_08710"
     CDS             complement(995..1306)
                     /locus_tag="PLQ68_08710"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXK72772.1"
                     /translation="MDITYNDTKKDLPVGQLHDLFISAGWSDGSESKVMIEKYNIPFI
                     NSTLVISAWDNEHLVGVVRVLSDKIFRSVIYDLVILPAYQNQKIGKELLKRCIRALSK
                     F"
     gene            complement(1370..2272)
                     /locus_tag="PLQ68_08715"
     CDS             complement(1370..2272)
                     /locus_tag="PLQ68_08715"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HXK72773.1"
                     /translation="MLDGNINNYISSDKKTVLTVRYQANRNLSKMQVSFHWWAETYTS
                     LHKHDHYEFFIITAGHIQHTLNGNTEILAENTLQLIRPDDVHQFSQLEGCKSTHINIS
                     ATCERFEKLCNALGISIDQLLNGNCPLRFELSTNEMDFFMHRAQQLNLQMRDDDTDCS
                     LFQLTVCEMLIHAISILYKRHPMRIDDNPKWLSTLLKQLHSPDMMSCTAEDVYKLSGY
                     SPPVVIKKFKQYTGQTVQSYLVKLKIDWAIILLQTTDKTVLEISEELGYSSLSHFCKL
                     FRAQTGETPKKFRTFDKNVSFKEN"
     gene            2449..3789
                     /locus_tag="PLQ68_08720"
     CDS             2449..3789
                     /locus_tag="PLQ68_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016524313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXK72774.1"
                     /translation="MRKDSKLTARPTKPINYAIGMFGTSIPINMFKTFAFVFYVDHLA
                     VLTAEKFALIIAAYTILDAIDNPVYGFLSDSTRSRWGRRRPWLVIGAPLLALSFIMFF
                     SVPAGLSEGSVFWYALIMYMFTGTLDSMINSNYGALFPELFKTETQRAKTNALRQVFQ
                     LVAMIVSIALTPIVTEKIGYRNTSIVYSILAVVVILYMALNSHETPEAQKMPKPNLFK
                     TVWDILRNPKFWIYGLSNAAFFAALAILQQSVSLYSKYVLNEGGLASTIMLASVIVCA
                     ILGIPVWVKIMKKLKLMKTWRLSMVFVAVSLIPLFFVNTLVATIVSLVVLGFGYGGSC
                     VTMDIVASRILDEDTLRHGVKREGTFSSLIGVLNKTSNLFVAGGFLIVSRVYGYQNGD
                     FPGPDPSSAARFLLSLFPFIVMIFCVIMSLFLNFKNLPEQQTVAVEESETKKEQ"
     gene            3789..4553
                     /locus_tag="PLQ68_08725"
     CDS             3789..4553
                     /locus_tag="PLQ68_08725"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HXK72775.1"
                     /translation="MAVITLNFRSIALKMSTTVTVVLPDSVRIGDTPISERKCLYLLH
                     GLSDDSSSCLRLSKIELYAQEAGIVIIMPSAGRSMYCDNIYGQNYFSYIAHELPEYMN
                     LVFGIKADKDKIFIAGISMGGLGASRIALSYPERFSAVGLFSGLLNLKYMLPVMTDEQ
                     KNDFSFMLSEVKDIDTSPLNPVNLLDAKKHANLKFYIYCGKQDDLFPLSISFIEKART
                     LGLKVISCIEDGIHDWYFWDKHLKTFIEDISQNKDE"
     gene            4546..5826
                     /locus_tag="PLQ68_08730"
     CDS             4546..5826
                     /locus_tag="PLQ68_08730"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HXK72776.1"
                     /translation="MNKHNILHHIPKTKEGTYYTIDFPMPHGMEIVTVAYSYKRFRGK
                     SLRLSKMVNIVDIGLIDADNRFIGWSGSAKSSVFTGQYTTIQGYAMVPLKPGVWKIIV
                     GAYKIPDEGLDVSYEISYKKSEARWFIGDLHMHSNASDGKHDIFTLTQMASKKKLDFI
                     AVSNHNNYSENMHLPVIPGLTLIPAVEWTHYLGHMNFFGVKAPFINSFIANNEEEMRN
                     LVSDAKSLGAKISVNHPKCNICPYLWKSDDVFDIIEVWNGPMRKVNMDAIKWWHEMLL
                     SGKKIPIIGGSDFHKKHHIVRMANPVTSVYADSPSKEDILNAISNGHSYITSSVKGVS
                     LKLSYNEAMMGDTAKYQSEQKISVSADNLKAGISVRLVSNHGTISEWKHFKKGKLKAE
                     IQIPEKCSFVYLIAQRNVLGQVFCCAISNPIYFE"
     gene            5911..8823
                     /locus_tag="PLQ68_08735"
     CDS             5911..8823
                     /locus_tag="PLQ68_08735"
                     /inference="COORDINATES: protein motif:HMM:NF013256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HXK72777.1"
                     /translation="MKSLKKETLHLARLRNIRKAIYIKIATLDVAVHTSKEPIPPGTQ
                     KDLNYTSISQKRIWAGVYGCAWFRIKGTVPKNAAGCHVVIKICIGGEGAVYDDCEPIS
                     AITSVLGYAERIGASKGKSIIEISDTAIPNTPVEIYMDAGYNGYYNFPYGYGIFKYAY
                     LCIVNDDLKDYYYDYLCLASLLSVTKDTKRRKEINDLLNKSYAIYRTSIASAREILSS
                     QFQSEPEENIDFTAIGHSHLDLAWLWPIRETKRKAQRTFANQINNSSKYPEYIYGASQ
                     PWQFDYIKNNFPKQYSMLKKKVSEDKFELQGGMWVEADMNLSSGEAIIRQILYGKEFF
                     KNEFDKDMLMCWLPDVFGYNGNLPQLLKKSGNPYFMTTKLSWNEHNRFPYRSFIWKGI
                     DDSEVLVHMPPDDTYNSAGSPACARHGADNYSERDKTPHALMVHGIGDGGGGPGDIHI
                     ELLSRQKSLKGSPKVTMGKAIDFFQKLNMYRENLPKYKGELYFEKHQGTYTTQGKTKY
                     YNRRSEYSLQTLEALCTLAWKKGMEYPKDKLDKWWKEILLYQFHDILPGSSVNRVYTE
                     SHQRYEEILNEIANEKEKVLEFLKTNEGISVFNPTSFVRKEFIKIDDTWYHSTVEPYA
                     FSTVQPVKDYNVSFSDNIMENNYIKLVFSKDGDISSAINKKTNREYAKENLNTLRIYK
                     DKWRFFNAWDIDWKYYKKHSKVLKAYKSEIFIDGPSVIRRSFYKHRNTTITQDVILYD
                     NSPVVYFKTNCNYHETFKMLRADFDFAVNSSFVSCDIQLGTITRSTGNTTDIEKAQFE
                     ICAHKYVDVSEKTHGISIMNDCKYGYRSKNNRISLCLLRSPVFPDKTADKGEHSFTYA
                     MYLHDNELGVDTLAYSYELNKPLEVIRHATDSISLASTDNENVVIETIKKSHTDDGII
                     LRLFESQGKPAICSINTIFKHTEVFETDLMENELKKIDITSLSFTPFEIKTIKLKW"
     gene            8830..9477
                     /locus_tag="PLQ68_08740"
     CDS             8830..9477
                     /locus_tag="PLQ68_08740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72778.1"
                     /translation="MKNLNTMLSKNYLYRLAISIFGIGIISLASALFFLTDLGADPYQ
                     VLCNAIHIQFSITIGMANMILNGTIVIFILIFFRKFINIAMFLSLLLSGLIIDGFVTI
                     LGPIVNQNMPMAVKILIVILACILLSFGVYLYTAPSLGASPADSVGLIIAKLVKKPYS
                     LIRIFTDVFYTATGFLLGGKVGITTIISVLLTGVTIGVFEKLLDKTKLIRYVKKN"
     gene            complement(9772..10035)
                     /locus_tag="PLQ68_08745"
     CDS             complement(9772..10035)
                     /locus_tag="PLQ68_08745"
                     /inference="COORDINATES: protein motif:HMM:TIGR01439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HXK72779.1"
                     /translation="MIIELRKKSQITLPKEIVRELNLVEGDKFEVNVVNSQILLEPVA
                     VYPKEYLKKIEEEIMILKEDSNFKYNAFDNLDDLMKNLKNNGN"
BASE COUNT         3354 a         1772 c         1756 g         3243 t
ORIGIN      
        1 tcccatctgc aggtttttca gcattaaacg gtatcttata tgattttcca tcttttataa
       61 cacctgaaat cttaccctca ctgcaaagaa cacgtacacg gcgatctgaa atattccatt
      121 tatctgctgc ttgctgagca gttatatatc cgctttccat taagttcact cccaaaagac
      181 aatatatcac tcaatcggaa taatgtcaat tcatatcatt ccgtataact tatcattata
      241 tagcaaagaa atttttcact gacaaaaaat cgcttaacaa aataaaaaat acattattaa
      301 ttcgtattat caacataaat ttgtattagt ttaataaaaa atagtgctaa agactattta
      361 accttcatca ttatatattc tgtaatacct tttcgatata atcctggaat ttctccaacc
      421 tgacaatatc caatactttc ataaaagcgt tttgcttttt cattgtagtc agctaccgct
      481 aagaaaaact tatctttacc agatatgttt tcaaaaaact caacaagctg cctacctatt
      541 cctttacctc tatattcttc cttaacggca atgagatgca aatatggata actatgaaat
      601 gctccattgg taatatagaa aaagaatcca acgcaaatgt tgtctgtaat tgcaacataa
      661 agattaccct tatctaatgc ttcttcgatt gccttaagcg cacttttctc actggaaaaa
      721 tattgctctc caagtgaaga tttcaaaaga gcttcttcgc aatcctttat atattcacat
      781 gttcccttta caatttttat attcattaac atctcctaac gtactttatc ataaaattat
      841 actcttttgt cattatgtct gagcaataca aaagtaatac ttttatttca ctgtaaacaa
      901 tttacaagct attgttaaaa aggtatcttt actttctgta aaaccattct tctcataaaa
      961 aactgaaatt tcctttgttg tctgaactag ccattcagaa tttggataat gctcttatac
     1021 atcttttaag cagttcttta cctattttct gattctggta tgctggaagt ataacaaggt
     1081 catatatcac ggacctgaat attttatcac ttaagacacg aacaaccccg actagatgct
     1141 cattatccca ggcagaaata accaaagtag agttaataaa cggaatgtta tatttctcaa
     1201 tcattacttt gctttccgat ccatctgacc agccagcaga tatgaataaa tcatgcaatt
     1261 gtccaacagg taaatctttt tttgtgtcat tgtaagttat atccatacta tttttccttc
     1321 actatttata attatgattt tatgtgtatg cattttctat ttatataatt taattttctt
     1381 taaaagaaac atttttatca aaagtcctga atttttttgg tgtctctcca gtctgcgctc
     1441 taaaaagctt acagaaatga ctaagacttg agtaaccaag ctcttcagat atttccaaaa
     1501 cagtcttatc tgttgtttgc aaaaggataa ttgcccagtc gattttcaat ttaaccagat
     1561 atgactgaac agtctgtccg gtatactgct tgaacttttt aattactact ggaggcgaat
     1621 atcccgaaag tttatagaca tcctctgcag tacagctcat catatcggga gaatgaagct
     1681 gctttaataa cgtggagagc cacttagggt tatcatcaat acgcattgga tgtcgtttat
     1741 atagaattga aatagcatga ataagcattt cacatacagt cagctggaat aagctgcagt
     1801 ctgtgtcatc atctctcatc tgaagattta gctgctgtgc tcgatgcata aagaaatcca
     1861 tttcatttgt gcttaattca aaacgtaacg gacagtttcc atttaacagc tggtctatac
     1921 ttatacccaa agcgttgcaa agtttttcaa atcgctcgca ggtagcagaa atatttatat
     1981 gagtactttt acagccttca agttgagaaa actgatgaac atcatccggc cgtatcagct
     2041 gcaatgtgtt ttctgcaaga atttcagtgt ttccattaag agtgtgctgg atgtgaccag
     2101 cagttatgat aaaaaattcg tagtggtcat gcttatgtag tgaagtatat gtttcagccc
     2161 accagtgaaa cgagacctgc atcttcgaaa ggttgcgatt tgcctgataa cgcacagtta
     2221 acacagtttt tttgtcagaa ctaatgtaat tattgatatt tccgtcaagc attattgaaa
     2281 cctctcatat gccaagcgta acagtaaaag aaagttttta caatagtaaa cagttgtaaa
     2341 ttcgcaatac agttaaaaaa atcgctcata ttattcaact aatcaataga caggcaaatt
     2401 gcgcagggat ataattcata taagggatat aatcgaaagg agaatatgat gagaaaggac
     2461 tcaaagttaa cggctcgtcc tacaaagcct attaattatg caataggaat gtttggtaca
     2521 tccattccta ttaatatgtt caaaacattt gcttttgttt tttatgtaga tcatcttgca
     2581 gtactgactg ctgaaaaatt cgcgcttata attgcagctt atacgatttt agatgcgata
     2641 gataatcctg tttatggatt tttgtcagac agtacccgat cgcgttgggg tcgtcgccgg
     2701 ccatggctgg ttatcggtgc accgcttttg gcattatcat tcataatgtt tttctctgta
     2761 cctgcaggtt tgtcagaggg ctccgtgttc tggtatgcgc ttataatgta tatgtttaca
     2821 ggcacattgg attcaatgat aaattccaat tacggcgcac tgtttccaga gttatttaaa
     2881 actgagacgc aaagagcaaa aaccaatgct cttcgacagg tattccagct tgtagccatg
     2941 atagtaagta tagcattaac acctattgtt actgaaaaga taggttacag aaacacttcc
     3001 attgtctaca gcatactagc tgtggtagta attctgtata tggctttgaa ttctcatgaa
     3061 acaccagaag ctcagaaaat gcctaaacca aatcttttca aaactgtatg ggatatattg
     3121 cgtaatccca agttctggat atatgggctt agcaatgctg cattcttcgc agccttagcg
     3181 attttacagc aatctgtttc tctatattca aaatatgtac ttaatgaggg cggacttgca
     3241 tctactatta tgcttgcatc tgtaatagtt tgcgcgattt taggtatacc tgtctgggtt
     3301 aaaataatga aaaaactcaa actgatgaaa acatggaggc tttccatggt attcgttgca
     3361 gtttcactca tacctctatt ctttgtaaat actcttgttg caaccatagt ttcacttgtt
     3421 gtacttggct ttggctatgg aggctcatgc gtaacaatgg atattgttgc ctctcgaatt
     3481 cttgatgaag ataccttacg acacggagtc aagcgtgaag gaactttttc cagtcttatc
     3541 ggagtactga acaaaacaag caatcttttt gttgcaggag ggtttttgat tgtgtctaga
     3601 gtttatggct atcaaaatgg tgattttccc ggccccgatc cttccagtgc agcacgtttc
     3661 ttgctaagtt tgttcccttt catagtaatg attttctgtg tcataatgtc attgttctta
     3721 aatttcaaga atttacctga acaacaaaca gttgctgttg aagaatctga aactaaaaag
     3781 gagcagtaat ggcagttata actttgaatt tccgatcaat cgcattgaaa atgagtacca
     3841 cagttacagt agtacttcca gattcagttc gtattggaga tacacctata tctgaacgta
     3901 agtgtttgta tttactacac ggtctatctg atgattcatc ttcctgctta agactgtcca
     3961 aaatcgaact ttatgctcag gaagcaggaa ttgtaatcat aatgccaagc gcaggtcgaa
     4021 gtatgtactg cgataatatt tatgggcaga actattttag ctatattgct catgaactgc
     4081 ctgaatatat gaatcttgta tttggtataa aagcagataa agataagata tttatcgcag
     4141 gcatatctat gggcggtctt ggagcatcgc gtattgctct aagttatcca gaacggtttt
     4201 ctgctgtagg attgttttca ggtcttttaa accttaaata catgctgcca gtaatgaccg
     4261 atgaacagaa gaacgatttt tcattcatgc tttctgaagt taaggatatt gatacttccc
     4321 cacttaaccc tgtaaacctt ctggatgcca aaaagcatgc taacctgaaa ttctatatct
     4381 actgcggaaa acaagatgat cttttcccct taagtataag cttcattgaa aaagcaagaa
     4441 ctctgggatt aaaagttata tcctgtatag aagacggtat acatgactgg tatttctggg
     4501 ataagcatct taaaacattt atagaggata tttcacagaa caaagatgaa taagcataat
     4561 atattgcatc atattcctaa aaccaaggaa ggcacatact ataccataga ttttccaatg
     4621 cctcatggaa tggaaattgt aactgttgca tatagctata aacggttcag aggaaaatcc
     4681 ttacgtttaa gtaaaatggt taatattgtt gacattggcc ttattgatgc tgataaccga
     4741 tttatcggct ggtcaggaag tgctaaaagc agtgttttta caggacaata tactacaata
     4801 cagggttacg ccatggtacc tttaaaacct ggtgtatgga agataattgt tggagcatac
     4861 aaaatacccg atgaaggatt ggatgtatcc tatgaaatat cttacaaaaa aagcgaagca
     4921 agatggttta taggtgattt gcacatgcat tccaatgcat ctgacggcaa acatgatatt
     4981 tttacgctca cacagatggc ttcaaaaaag aagctggatt ttatagcagt ttcaaaccac
     5041 aataattaca gtgaaaatat gcatcttcca gttatacccg gccttacttt aattcccgct
     5101 gtggaatgga ctcattacct tggtcatatg aacttttttg gagtaaaagc tccttttatc
     5161 aattcattca ttgctaataa tgaagaagaa atgagaaatc ttgtttctga tgcaaagagc
     5221 ttgggtgcaa aaatatcagt taatcatcca aaatgcaaca tatgcccata tttatggaaa
     5281 agtgatgatg tttttgacat tatagaagta tggaatggcc ctatgcgcaa agttaatatg
     5341 gatgccataa agtggtggca tgaaatgctt ttaagcggga aaaaaattcc aataatcggt
     5401 ggaagtgatt ttcacaagaa acaccatata gttcggatgg caaatcctgt tacatccgtg
     5461 tatgctgatt caccttctaa agaagatata ctaaatgcaa ttagtaatgg acacagctat
     5521 atcacatcat cagtaaaagg tgtgagttta aagctatctt ataatgaagc aatgatggga
     5581 gatacagcaa aataccaatc agagcaaaaa atttcagttt cagcggataa tttaaaagca
     5641 ggcatatctg tcagactagt ttcaaatcat gggactatat ccgaatggaa gcattttaaa
     5701 aaaggaaaac taaaagcaga aatccaaatt cctgaaaaat gcagttttgt gtacctaata
     5761 gcgcaacgta atgttttagg acaggttttt tgttgtgcaa tttcaaatcc tatttatttt
     5821 gaataagtaa taatataata tatataaata tcagatggtt atataaaaat atcttttagg
     5881 tgcttaaggg agaattgaaa attggaatcg gtgaaaagtc ttaaaaagga aacactgcat
     5941 ttagcaaggt taagaaatat ccgtaaggct atatatatta aaatagctac tcttgatgta
     6001 gcagttcata caagcaaaga accaattcca cctggaacgc aaaaagactt aaattacacc
     6061 tctatttccc aaaaaagaat ctgggcagga gtatatggtt gcgcatggtt tcgaataaaa
     6121 ggtactgtac cgaaaaatgc tgcaggttgt catgtagtga ttaagatatg cataggagga
     6181 gaaggtgcag tttatgatga ttgcgaacca ataagcgcaa taacctcagt tttaggctat
     6241 gctgaacgaa ttggagcttc caaaggaaaa agcataattg aaatatccga tactgctata
     6301 ccaaatacac cagtagaaat atatatggat gctgggtata acggatatta caactttcca
     6361 tacggatatg ggatatttaa atatgcttat ctatgtatcg tcaatgacga tttaaaagat
     6421 tactactatg actatttatg tcttgcatct ttgctttcag ttacaaagga caccaaaaga
     6481 cgtaaagaga ttaatgattt attaaataaa agttatgcta tatatcgcac ttctatagct
     6541 tcagcaagag agattttaag ttctcagttc cagagcgaac cagaagagaa tattgacttt
     6601 acagccatcg gccattctca tttggatctt gcctggctgt ggccgattag agaaacaaaa
     6661 cgcaaagcgc aacgtacttt tgcaaatcag ataaataact caagcaagta tccagaatat
     6721 atttacggag caagccagcc atggcagttt gattatatta aaaacaactt ccccaaacag
     6781 tactctatgc ttaaaaagaa ggtttctgag gacaaatttg agcttcaagg cggtatgtgg
     6841 gtagaagcgg atatgaactt aagttccggt gaagctatta tccgtcagat actgtacgga
     6901 aaggaatttt tcaagaatga atttgataaa gatatgctga tgtgctggtt gccggatgta
     6961 ttcggttata acgggaatct tccacagctc ttgaaaaaaa gcgggaatcc ctacttcatg
     7021 actactaaat tatcatggaa tgagcacaat cgcttcccct atcgcagctt tatatggaaa
     7081 ggcatcgatg acagtgaagt acttgtccat atgcctcctg acgacactta caacagtgca
     7141 ggaagtcctg cttgcgcacg tcatggtgcg gataattatt ccgaacgtga caaaactcct
     7201 catgctctta tggttcatgg tattggcgat ggtggaggcg gacctggaga tatacatatt
     7261 gaattacttt caagacaaaa aagtcttaaa ggaagtccaa aagtaacaat gggcaaggct
     7321 attgatttct tccaaaaact gaatatgtac agagaaaacc ttccaaaata caaaggcgag
     7381 ttatactttg aaaaacacca aggcacatat acaacacagg gtaaaactaa atactataac
     7441 cgtaggtcag aatattcttt acagacttta gaagcattgt gcacgcttgc ctggaaaaaa
     7501 ggtatggaat accctaagga caaactggac aaatggtgga aagaaatact gctatatcag
     7561 ttccatgaca ttttacctgg aagcagcgta aatagagtct atactgaaag ccatcaaaga
     7621 tatgaagaga ttttaaatga aattgcaaat gaaaaagaaa aggttcttga atttttgaag
     7681 acgaatgaag gaataagcgt atttaatccg acttcattcg tacgaaaaga atttatcaag
     7741 attgatgaca catggtatca ttccactgtc gaaccatatg cattttctac agttcaacct
     7801 gttaaagact acaatgtaag tttctctgat aatattatgg aaaacaatta tatcaagctt
     7861 gttttttcga aagatgggga tatatcatca gcgattaata aaaagaccaa tagggaatat
     7921 gcaaaagaaa atcttaatac tcttcgaatt tacaaggaca aatggcgatt cttcaatgcc
     7981 tgggatattg actggaaata ctacaaaaag catagcaaag ttttaaaagc ttacaagagt
     8041 gaaatcttta tagacggacc gagtgtcata cgacgtagct tctataaaca cagaaacact
     8101 acaatcacac aggatgtcat actgtatgat aacagcccgg ttgtgtattt caaaaccaac
     8161 tgcaattatc atgaaacttt caaaatgcta agagcagatt ttgattttgc agttaacagc
     8221 tcctttgtaa gctgcgacat ccagctagga acaataacaa gaagtactgg aaacactact
     8281 gatattgaaa aagcacaatt tgaaatatgc gcccataagt atgtcgatgt ttccgaaaaa
     8341 acacatggga tatctataat gaatgactgt aaatacggat acagatcaaa aaacaaccgt
     8401 atatctttat gtctgctacg ctcccctgtt tttcctgaca aaactgctga taaaggtgag
     8461 cattcattca cttatgcaat gtatttgcat gataatgagc ttggagttga taccttggct
     8521 tattcctatg aacttaataa acctttggaa gttattcgac atgcaactga cagcatcagt
     8581 ttagcaagta cagataatga aaatgtcgta atagagacaa tcaagaagtc acataccgat
     8641 gatggtataa tcttacgctt atttgagagc cagggaaaac cagcaatatg cagcattaat
     8701 accatattta aacatactga agttttcgag actgacctta tggaaaatga attaaagaaa
     8761 atagatataa caagcctttc attcacacca tttgaaatta aaacgattaa gttgaaatgg
     8821 taatagtaaa tgaagaatct taatactatg ctatccaaga attatctata ccgccttgct
     8881 atttctatat tcggaatagg tataataagt cttgctagtg cgttattctt tttaactgac
     8941 cttggagcag atccatatca ggtgctgtgt aatgcaattc atattcagtt ttcaatcaca
     9001 ataggcatgg caaacatgat attaaatggg acaatagtaa tatttatcct tatcttcttt
     9061 agaaaattta taaatatagc tatgttttta agcttattgc tttcaggtct tattatcgat
     9121 ggatttgtga ctattctagg accaattgtt aatcagaata tgcctatggc tgtaaaaata
     9181 ctaatagtta ttttagcttg tattctatta tccttcggag tatatcttta taccgcgcca
     9241 tctcttggag caagtcccgc tgacagtgta ggcttaataa tcgctaaact tgtcaaaaaa
     9301 ccttattcac ttatacgtat ctttactgat gtattttaca cagcaacagg atttctttta
     9361 ggcggcaagg taggtattac aacaattata tccgtattgc taacaggagt gactataggg
     9421 gtatttgaaa agctgctgga taagaccaag ctgataaggt atgtaaaaaa gaattgatta
     9481 gttttataaa gctatttcat gtcttacaaa ttcctcaaaa tatcgtggta accaatgtca
     9541 ataagtatta tgagatgttg gtcttcataa taccaaataa tcctattatc cgtatttata
     9601 ctgctttcat acatatcctt atgccctata attttctttg ttcgcaatga aggatgcatg
     9661 ttatttttaa caaatatctt catcttgttc tcaaatgcag tttgttcaac aatcgttaat
     9721 ctgttaaatg acttcaaaaa tcgatttgta taagttattt taaacgaatt attagtttcc
     9781 attatttttt aaatttttca ttaagtcatc taaattatca aatgcattat atttgaaatt
     9841 agaatcctct ttaagtatca ttatctcttc ttctatcttc ttaagatatt cttttggata
     9901 tactgctaca ggttccaata aaatctgact attaactaca tttacttcaa atttatctcc
     9961 ttcaactaag tttaactctc tcacgatttc ttttggtagt gttatctgtg atttttttct
    10021 taattcaatt atcatagcat cctccggttg gaatttctta ctttctaact acattatatg
    10081 ataattcata taatttgcaa cccatttatt ctttttaaaa cgtta
//
LOCUS       DAOSXM010000074        10123 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2111
            NODE_4195_length_10123_cov_11.700238, whole genome shotgun
            sequence.
ACCESSION   DAOSXM010000074 DAOSXM010000000
VERSION     DAOSXM010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864178
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 10123)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10123)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:24:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,261
            CDSs (total)                      :: 2,229
            Genes (coding)                    :: 2,218
            CDSs (with protein)               :: 2,218
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 3 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10123
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4195_length_10123_cov_11.700238"
                     /isolate="AMR_MDS_2111"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..607)
                     /locus_tag="PLQ68_08750"
     CDS             complement(<1..607)
                     /locus_tag="PLQ68_08750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009170237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HXK72780.1"
                     /translation="MSKHITKEWDWSKNKDNKWLVPCTDSAYLAERWKSKEFRSLLDL
                     GCGLGRHSIYMASHGFDVTAVDLSEYGINNLNEWAKKEKLDIKSCVGNMLSLPFENNS
                     FDCMIAYNVIYHTDTNGFIETLKEIKRVLKPEGELYITLISKNTYSYIYSKEEKRIDE
                     NTYMRDEDDTEKNVPHFYVNHRDIQKFFTDWEFELKPVEIAE"
     gene            complement(630..1076)
                     /locus_tag="PLQ68_08755"
     CDS             complement(630..1076)
                     /locus_tag="PLQ68_08755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010283648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXK72781.1"
                     /translation="MSIEVKAATIDDIDNITALGLLLYSLDNTYESLYDENTELLKSE
                     ADNIFLAFDDDTAVGFSHCSLRTDYVEGTTGGRVGYLEGIYVNPDYRNKGIARKLIKS
                     CETWAKEKGCKEFASDCLIENTESYKFHMKMGFKEANRIICFTKEL"
     gene            complement(1164..2279)
                     /locus_tag="PLQ68_08760"
     CDS             complement(1164..2279)
                     /locus_tag="PLQ68_08760"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HXK72782.1"
                     /translation="MHIKYPEYDKSLLSLASSILKHYGAESTHASYSYVDNLIAKDYM
                     NVVIMLFDGLGTKILEKHLPEDSFLRKHFVESFSSVFPPTTTAATVSIESGLSPAEHG
                     WLGWSLYFSSIDTTVSIFPNTISGSKGVQAAQYHVARKFLPYKNIFTKIKEATDNKVQ
                     AICVSPYSAYESKTVNQICMTVNDLCHQPGKKYIYTYWPQPDYDMHDKGTKHLRIRKC
                     IEKINEEVESLCSNVENTLFIITADHGLVDTNWLFISDYPDLCECLLRNPCIETRALT
                     FFIKEGKEKVFEELFNKYFSKYYLLLTKKQVKEMYLFGKGKEHPLFDELMGDYLAIAT
                     GNISIDMTPNPNHPMFKAAHAGLTSDEMEIPLIVVQR"
     gene            2629..2982
                     /locus_tag="PLQ68_08765"
     CDS             2629..2982
                     /locus_tag="PLQ68_08765"
                     /inference="COORDINATES: protein motif:HMM:NF024949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HXK72783.1"
                     /translation="MENTLIDLGTKLKILREQSSFTQKNIAVFLNVDQSLISKMENGE
                     RSIPVEVLEQLASLYGVTLSFFEEGEKTVHPTTIALRSDEIDEKVFKTISIVNKIALN
                     CSFITSLLKEYQHNE"
     gene            2975..3724
                     /locus_tag="PLQ68_08770"
     CDS             2975..3724
                     /locus_tag="PLQ68_08770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020072904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HXK72784.1"
                     /translation="MNKMDLLLKANDLRTRFGEDFKSPVDIMSLAHSIDNLTIVLYPM
                     GEHLSGMCIKSNDNPVIAINSGMSMGRQHFSMAHELFHLYYDKSTTSICSKKIGYGKE
                     IEKQADQFASYFLIPPNALKDKIKQLKIDRERTLSIEDIVKIEQYFMVSRQAILFRLI
                     EDNEISIHDVEEMKQNVIQSATCFGYSDTLYKPLPESKQYMTYGYLIKQTNEAYKSKL
                     ISTGKYNEILLSAFRADIVYGTEEEEGEIVD"
     gene            3717..4262
                     /locus_tag="PLQ68_08775"
     CDS             3717..4262
                     /locus_tag="PLQ68_08775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020072903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72785.1"
                     /translation="MTDYIFFDTDCLSSFLWVNNQNFLVKLYSGRIYIPMQVYNELSN
                     PCVLHLKAKIDDILSKNHAKQISINTGTTEYSLYYQLTEKPAKGKYAIGKGEASALVL
                     AKKYNGTIASNNLSDISTYIYDFDIKHLTTADILYDALISNYITEDEGNKIWNAMQKK
                     RFKLGDLSFTEHINNQRITNT"
     gene            4347..5783
                     /locus_tag="PLQ68_08780"
     CDS             4347..5783
                     /locus_tag="PLQ68_08780"
                     /inference="COORDINATES: protein motif:HMM:NF027442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4832 domain-containing protein"
                     /protein_id="HXK72786.1"
                     /translation="MKYDFPNKVDFTPFKNNATQVCLYKPLKDVKGYQSLHLLEDDKR
                     IIKNPHKGWYWHYIDNSFKRGQYRDLHNSNDHLEDFPGLNHLYLRFDWGDIEIEDGVY
                     DWSYIDSIMNQWGSYGYHFALRICTYEGNAQIPFATPKFVYEKGARCYSLSNGNLEPD
                     YNDPIFLFYLERFLAKAGEKFNNNHLIETIDVGSFGTWGEGHTAAGTNIQYSADVIIK
                     HMELHSKYFPNKFVMFNDDFVNSCWERGIDENIRIINRAAELKMGIDDDSVQVSCYSR
                     DCGYTSLRSPWIFDYFNKHAPVTLELEHYVLVSEDNLKSGFPFIEAMRQTKATFAGFH
                     GYPRPWLEQNRYMTEYAANRLGYWYFINGITVNNGNDIKTDKDNEVTLFIENRGFAPA
                     YHKYDLKIALVNQNKSYIYPTDADNTEWLENRETEVKLAIHPIDIPKGDYKLAVGLFE
                     NDTPVEFGLKKEVYDEYGYAHISSVRVI"
     gene            complement(5849..7162)
                     /locus_tag="PLQ68_08785"
     CDS             complement(5849..7162)
                     /locus_tag="PLQ68_08785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002851746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXK72787.1"
                     /translation="MIDTHNLKRRDLYLNKIIAFQDTEPVKVVTGIRRCGKSSLLKLM
                     AAHLSETGIEDNQIIEMNFESYEFKKMTADELYYYVKQRVISGRRMYLFFDELQRITG
                     WEDTINAFRVDFNCDIYVTGSNAHLLSSEYSTYLSGRYVEIKMLPLSFSEFLYFHDFE
                     VKETISAIGGTRKRVFYKSGELYELKELFNTYMRFGGMPGIADVGLDIEKAMVLLDGI
                     YSTVVIRDILEREKRRGQRQIADPILLRKIILFLADNIGSNISVSSIGNILINEGLLD
                     DSKRKGAPSAHTVQAYVNTLLESYFFYDIKRFDIKGKEFLQTLGKYYIVDIGLRNYLL
                     GLRNRDSGHALENVVYLELIRRGYDVAIGKVDKSEVDFIATKTDDKLYIQVTESMTNE
                     DVRHRELVPLQKINDNYEKIVLSLDTGLDESYDGIKSINLIDWLI"
     gene            complement(7418..8287)
                     /locus_tag="PLQ68_08790"
     CDS             complement(7418..8287)
                     /locus_tag="PLQ68_08790"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HXK72788.1"
                     /translation="MKKNRKGNFIYDVDFIEHILKDLPSNIFFKDTECRYVFCTQYWR
                     HLKTDESDDWTIRGKTDLEVRVDKENARKAYEEDKKILKTGRGTEYVIEINQDGITEY
                     LEIIKRPVRDKNNEIIGIVGLINDVTDKVLMEMEIKKRANTDMLTGLGNRDSMMRWMS
                     EVLPSEQFPIGIVITDCDRLKEINDTYGHITGDEFIKMTGVVLKESFPEKAKIFRSGG
                     DEFLIAIPGTELDECLAYMDKAEKKGVNINGCNMSFSSGASIVRSPEEDIKKVMHQAD
                     SLMYLKKKKKRKM"
     gene            complement(8447..>10123)
                     /locus_tag="PLQ68_08795"
     CDS             complement(8447..>10123)
                     /locus_tag="PLQ68_08795"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HXK72789.1"
                     /translation="GKLIGVTSAMEYKLSTDTVWTSGTGSDITGLTDGTYHVRAKAAG
                     TILASENQILVINEYGIVRENTPNAIVDYISECLTGLNPNAAYLLNSGNVTSNANGEI
                     AISSEWFGTAISLVKVGNMTTTSDSLPQSIILSARPTAPACSVTQPSASSATGVISGI
                     TATMQYSTDNGATWVNGDGNDVTSLSSGTVLVRYKAQAGTAPKGLNQSITITAYVPPV
                     NTYTPPELVTIIHNGDSCIASNLDELILKRKTLTVNGDKFAKLIFDTDALKGIDEQTS
                     GHVRFEIKDVSSNYQDNFPGKQVFTLTVSSGGKVITNFRGSVTVSLPYELKEAETADQ
                     VTVWYLASDGIMTEIPCIYNDKTRLATFTVPHFSQYVVGVKTVWENPYSDIGENNTYY
                     DAIKFVANNNLMQGVSLTEFGPDAILTRGMIVTILYRIEEEPVTEKTLLFTDVAEGMW
                     YTNAVLWAADKNIVNGYDGKFHPNDTLTREQMAAILYRYATYKEYDVTKTGDLSSYTD
                     KPSDWALTSVSWAVAEGLLNSNDGSLNLKGNISRGQIATVLKKFMENNAR"
BASE COUNT         3213 a         1860 c         1589 g         3461 t
ORIGIN      
        1 attcagctat ttcaacaggc ttgagttcaa actcccagtc agtaaaaaac ttttgtatat
       61 ccctgtgatt aacataaaaa tgaggtacat tcttttctgt atcatcctca tctctcatat
      121 atgtgttttc atcgattctc ttttcttctt ttgaatagat atagctatat gtgttttttg
      181 atattaatgt tatataaagc tctccttcag gttttaatac tctttttatt tctttaagag
      241 tttcgataaa accatttgtg tcagtatgat atataacatt atatgcaatc atacaatcaa
      301 aactgttatt ttcaaatggc agtgagagca tattgccgac gcagctcttt atatcaagtt
      361 tctccttttt tgcccattca tttaagttat ttattccata ctcggacaaa tccacagcag
      421 ttacatcaaa tccatgtgat gccatatata tggaatgtct accaagcccg cagcctagat
      481 caagtaaaga tctgaattct ttacttttcc atctttctgc aaggtaagca gaatcagtgc
      541 atggtacaag ccatttatta tccttattct tgctccagtc ccattcctta gttatgtgtt
      601 tgctcacttt tacctcttct tatttatatt tataactctt tagtaaaaca tattattcta
      661 tttgcttctt taaaacccat tttcatatgg aatttatagc tttctgtatt ctcgatcaga
      721 cagtcacttg caaattcctt acaacctttt tcttttgccc atgtttcaca agatttaatt
      781 aattttctcg caatcccttt attcctgtaa tcagggttta catatattcc ttcaagatat
      841 cctactctgc ccccagttgt accttcaaca taatcagtgc gtaaggaaca gtgcgaaaat
      901 ccgactgctg tatcatcatc aaatgctaaa aatatattat cagcttctga cttcaataat
      961 tcagtatttt catcataaag gctttcatat gtattatcta agctgtataa aagcaatcca
     1021 agagcagtta tattatctat atcatcaatt gttgcagcct ttacttctat actcataaca
     1081 tgtcctttta aatatttcaa aattaatatg gaaagtataa cataaatttt gtaatgcgat
     1141 aatacttggc tcacaccaca ttattatctt tgtacaacaa tcaaaggtat ctccatctca
     1201 tctgatgtaa gccctgcatg agctgccttg aacataggat gattaggatt tggggtcata
     1261 tctatactta tattacctgt cgctattgct aaatagtcac ccattagctc gtcaaaaaga
     1321 ggatgctctt ttccttttcc gaagagatac atctctttta cctgtttttt tgtcagcaaa
     1381 agatagtatt tgctgaaata cttattgaaa agctcttcaa aaaccttttc ttttccttct
     1441 tttataaaga acgtaagagc tcttgtctct atacaggggt ttcttaaaag gcattcacat
     1501 aagtcaggat aatcagatat aaacaaccag ttagtatcta ctaacccatg gtctgcagtg
     1561 ataatgaaca gagtattttc aacatttgaa catagtgatt ccacttcctc attaattttc
     1621 tcaatgcatt ttcttattct taaatgtttc gttcctttat catgcatatc ataatcaggc
     1681 tgaggccagt atgtatatat atatttcttg ccaggctgat ggcataagtc attaactgtc
     1741 atgcatatct ggttgacagt tttgctttca tatgcagaat atggtgatac gcatattgcc
     1801 tgcactttat tatcagtagc ttcctttatt tttgtgaata tgttcttgta tggtaaaaac
     1861 tttcttgcta catggtactg agctgcctga acgcctttag aaccggatat ggtattaggg
     1921 aaaatactta cagtagtatc tatcgaagag aagtataagc tccatccgag ccatccgtgt
     1981 tctgccggag aaagacctga ttcaatagat acagtagcag cagtagtcgt agggggaaat
     2041 acagaactga aactttccac gaagtgtttc cttagaaaac tatcttctgg taaatgtttt
     2101 tcaagaatct ttgttccaag tccgtcaaac aacataatta caacattcat atagtcttta
     2161 gcaatcaaat tatctacata tgaatatgat gcatgggtag actctgctcc ataatgcttc
     2221 aaaatagaag aggccagcga taaaaggctt ttatcatatt caggatactt tatatgcata
     2281 tatacctcct gcgtgttaga taataatatt attaacgata atccataaat ccaattatta
     2341 tatattatca catttacact gctgaaacca agtatagccg atatttaata tggtcataat
     2401 atgttcatat ttaactatgc aatatatgac aacatttcag ataatttaca taattaagaa
     2461 tgcatataat catattgtaa tgcctataac acaaaatttc tgcattacta cttctatatt
     2521 tgctataatc tattcttatc ctttatattt ttgtcatata atataagaat taatatcttt
     2581 acattatgac attgctgtta tataatcaat tgggacatgg aggaagatat ggagaataca
     2641 ttaatagatc taggcacaaa acttaagata ttgcgtgagc aaagcagttt tactcagaaa
     2701 aatatagctg tttttctgaa tgttgatcag agcttgatat caaaaatgga aaatggcgag
     2761 aggtcaattc ctgttgaggt ccttgaacaa ttagcatcgt tatatggagt aactttatct
     2821 tttttcgagg aaggtgaaaa aactgtacat cctactacta tcgcattaag atcagatgaa
     2881 atagacgaaa aagtttttaa aacaatcagt attgtgaata aaatagcttt aaattgcagt
     2941 ttcataacct ctcttctcaa ggagtatcag cataatgaat aaaatggatt tgcttctaaa
     3001 agcaaacgac ttaagaactc ggtttggtga agactttaaa tcccctgttg atattatgtc
     3061 acttgctcat tcaattgata atttaaccat tgttttatac ccaatgggag aacatttaag
     3121 cggtatgtgc attaaaagta atgataaccc tgttatagcc ataaattccg gtatgtccat
     3181 gggaagacaa cacttttcta tggctcatga attatttcac ctttattacg ataagagcac
     3241 aacatcaatt tgctcaaaaa aaataggata tggaaaagaa atagaaaaac aagcagatca
     3301 gtttgcttca tatttcttaa ttccccctaa tgcattgaaa gataaaataa aacagttaaa
     3361 gatagatcgg gaaagaactc tttctataga ggatatagta aagattgaac aatactttat
     3421 ggtgagcaga caagctattc ttttcagact gattgaagat aacgaaattt ccattcatga
     3481 tgttgaggaa atgaaacaaa atgtcattca atctgctaca tgttttggtt atagcgatac
     3541 actatacaaa ccccttcctg aatcaaaaca gtatatgaca tacggatatc tgattaagca
     3601 gactaatgag gcatataaaa gcaaactaat atctacaggt aagtataacg agattcttct
     3661 ttcagcattt agagctgata tcgtatatgg cactgaagaa gaggaaggag aaattgttga
     3721 ctgattatat tttctttgac actgattgct tatcttcatt tctatgggtt aataatcaga
     3781 actttctggt aaaactatat tcaggtagaa tatatatacc aatgcaggta tataatgaac
     3841 tttcaaatcc ttgcgttttg catctcaaag caaaaataga cgatatttta tccaagaatc
     3901 acgcgaaaca aataagcatt aatactggta caacagaata ttctctttat tatcagctaa
     3961 cggaaaaacc agccaaaggg aaatatgcaa taggtaaggg agaagcttct gcattagttt
     4021 tagcaaaaaa atataacgga acaattgcaa gcaataacct aagtgatata tcaacatata
     4081 tttacgattt cgacataaaa cacttaacta ctgcagacat cctgtatgat gcattaataa
     4141 gtaattatat aacagaagat gaaggaaata agatatggaa tgctatgcag aaaaaaaggt
     4201 tcaaattggg agatttatct tttactgaac atataaacaa tcaaagaata actaacactt
     4261 gacccctccc ttttctcaaa atcttattaa aatacctttc atatgtgtta ctattaatat
     4321 cagatgaaca ttcaggagga acatttatga aatatgattt tccaaataag gttgatttta
     4381 cgccttttaa gaataatgcc acacaggttt gtctatacaa accattaaaa gatgttaaag
     4441 gttaccaaag ccttcatctt ttggaagatg ataagaggat tataaagaac cctcataaag
     4501 ggtggtactg gcattatata gataacagtt ttaaaagagg acagtacagg gatttacata
     4561 attcaaacga ccatcttgaa gattttccag gtctaaatca tctttacctc cgttttgact
     4621 ggggagatat cgaaattgag gatggagtat atgactggtc atatatagat tcaataatga
     4681 atcaatgggg gagttacggt tatcattttg ctttacggat atgtacatac gagggaaatg
     4741 cacagatacc atttgcaaca cctaaattcg tatatgaaaa gggagctcgt tgctacagtt
     4801 tgtcaaacgg aaacttagaa ccagattaca atgacccaat ctttcttttt tatcttgaaa
     4861 gatttcttgc taaagcagga gagaaattca ataataatca tcttattgaa acaatagatg
     4921 tagggtcttt tggtacctgg ggcgaagggc atactgctgc aggcacaaat atccagtata
     4981 gcgctgatgt gataataaag cacatggaac ttcattcaaa atatttcccg aacaaatttg
     5041 tcatgttcaa tgatgatttt gtaaattcct gctgggaaag aggaattgat gaaaatataa
     5101 ggatcattaa tcgtgcagct gaactgaaaa tgggaattga tgatgattct gtgcaggtaa
     5161 gctgttattc cagagactgt gggtatacct cattacgaag cccttggatt tttgactact
     5221 ttaataaaca tgctcctgta actttggagc ttgaacatta tgtattagta agtgaagata
     5281 atttaaaatc aggattccca tttattgaag caatgagaca gactaaagcg acttttgcag
     5341 gattccatgg atacccaaga ccatggttag agcagaacag atacatgaca gaatatgcag
     5401 ccaacagact gggctactgg tactttataa acggaatcac agttaataat gggaatgata
     5461 taaaaacaga taaagataat gaggtcactt tgtttataga aaacagagga ttcgctcctg
     5521 catatcataa atatgacctg aagatagccc ttgtaaatca gaacaaatca tatatatatc
     5581 caactgacgc tgacaacaca gagtggcttg aaaatagaga aacagaagtc aaacttgcta
     5641 tacatcctat agatatccca aaaggtgatt ataagcttgc tgtcggatta tttgaaaatg
     5701 acacacctgt tgagttcgga cttaaaaagg aagtttatga cgaatatggc tatgcacata
     5761 ttagtagtgt aagggttata tagtgtagaa aacctgtcat ttacagacag gtttattaaa
     5821 tactaatctt caaaaacata ttacctgatt atataagcca gtcaatcaga ttaatcgatt
     5881 ttataccatc ataggattca tccagtcctg tatccagtga aaggactatc ttttcataat
     5941 tatcatttat tttttgcaaa ggaacaagtt ctctatgacg aacatcttcg tttgtcatgg
     6001 attctgttac ttgaatataa agtttatcat cagtttttgt agcgatgaaa tccacttcag
     6061 atttgtctac tttacctatt gccacatcgt aacctctgcg tattaattct aggtaaacta
     6121 cattttccaa agcatgaccg ctatcgcgat ttcttaatcc caacagatag ttgcgcaagc
     6181 ctatatctac aatatagtat ttaccaagcg tttgtaaaaa ttctttcccc ttgatatcaa
     6241 atcgcttgat atcataaaag aaatacgact caagcaatgt atttacatat gcctgtacgg
     6301 tatgagcgct aggagctcct tttcgcttac tatcatcaag taatccttca ttgattaaaa
     6361 tattaccaat agatgaaaca gaaatattgc tgccaatatt atctgcaaga aataaaataa
     6421 tttttcttag taagattgga tcagcaatct gcctctggcc tctacgtttt tctcgttcta
     6481 atatatcgcg aattacaact gttgaataaa taccatctaa cagaaccatt gctttttcaa
     6541 tatctagacc tacatctgct atcccaggca ttcccccaaa acgcatatag gtattaaaga
     6601 gttcctttag ttcataaagt tccccggatt tataaaaaac tcgcttacgt gtacccccta
     6661 ttgcgcttat tgtttcttta acttcaaaat catggaagta aagaaactcg ctgaaagata
     6721 gcggcagcat ctttatttcc acatatctac cagaaagata agtagaatat tctgaagata
     6781 ataagtgagc atttgaacca gtaacatata tatcacagtt aaagtctacg cgaaatgcat
     6841 tgatagtgtc ttcccatccg gtgatgcgtt gcaattcatc aaagaataaa tacatccgtc
     6901 ttccagaaat aactcgctgt tttacgtaat agtatagttc atcagcagtc attttcttga
     6961 attcgtagga ttcaaaattc atctctataa tttgattatc ttcaattcca gtttcgctca
     7021 aatgtgctgc cataagtttt agtaaactcg atttaccgca acgacgtatg ccagttacca
     7081 cttttacagg ttctgtatcc tgaaaggcaa tgattttatt tagatataaa tctcttctct
     7141 ttaaattatg agtatcaatc atggcaatac atctcctttt atgcaaatca tagcacaaac
     7201 tcatataaat tacaagtttg tgctattgat tgcaataact tacgcttttt cttgatttta
     7261 tgctattaat agcaaatatt tgaatgattc ttgcttgttc cttttcagaa aactcttcaa
     7321 cagcttcata cttctctata tgggtacttc tgtataaata ttagattttt aagaatttta
     7381 cttttattat cctgtatctt ttcttatatc agctttatta catcttacgc tttttcttct
     7441 ttttcaaata catcaatgaa tctgcttggt gcattacctt tttaatatct tcttctggcg
     7501 atctgactat tgatgctcct gatgaaaagc tcatattgca tccgttaata ttaacgcctt
     7561 tcttttctgc tttatccata tatgccaggc attcgtcaag ttctgttcca ggtattgcta
     7621 ttaggaattc atctcctccg gatctgaata ttttcgcttt ttctgggaaa ctttccttta
     7681 gaacaacacc tgtcattttt ataaactcat cacctgtaat atgcccatat gtatcattaa
     7741 tttctttcag tctgtcacag tcagttatga ctattccaat gggaaactgc tctgaaggca
     7801 atacttctga catccagcgc atcattgaat cacggttacc aagccctgta agcatatctg
     7861 tatttgctct tttttttatt tccatttcca ttaatacttt gtctgtaaca tcgtttataa
     7921 ggccgactat accaattatt tcgttattct tatctcttac agggcgtttt ataatctcga
     7981 gatattccgt tattccatct tggttaattt cgatgacata ctcagtacca cgtcctgttt
     8041 taaggatttt cttgtcttcc tcgtatgctt ttctggcatt ttctttatca acacggactt
     8101 caagatctgt tttacctctt atggtccagt catcactctc atccgttttt aaatgtctcc
     8161 aatattgagt gcaaaaaaca tatctgcatt cagtatcctt aaagaaaata tttgaaggaa
     8221 gatccttcaa tatatgctca atgaaatcca catcataaat aaagtttcct ttgcggttct
     8281 ttttcataaa agccctcctt ttggtcactt tattacttac cataatagta attattaatt
     8341 actttgtcaa ttcttgtaca ccctgatctc ataagctaga tgaaaccatt aaaaaaggag
     8401 gttgatattt aatcattcct ccatttttgc ttatttattt tattttttat ctagcattat
     8461 tttccatgaa tttctttaat acagttgcta tctgccctct ggatatgttc ccctttaaat
     8521 taaggcttcc atcgttgctg tttaacagtc cttctgcaac agcccagctt acagatgtaa
     8581 gcgcccagtc agaaggtttg tctgtataag atgataggtc tcctgtttta gtaacatcat
     8641 attccttgta agtagcatag cgatataaga ttgctgccat ctgttcacgg gtaagagtat
     8701 cattcggatg aaattttcca tcataaccat taacaatatt cttatctgct gcccataata
     8761 ccgcatttgt ataccacata ccttcagcaa catcagtaaa cagcaaagtc ttttctgtta
     8821 caggctcttc ttcaatcctg taaagaattg ttacaatcat tccacgagtg agaattgcat
     8881 caggaccgaa ctctgttaaa ctgactccct gcataagatt gttattggca acaaacttaa
     8941 ttgcatcgta atatgtattg ttttcaccta tatcagaata tggattttcc catactgttt
     9001 ttactccgac aacatattgt gagaaatgag gtacagtaaa agttgcaagt cttgttttat
     9061 cattgtatat acatgggatt tctgtcatta taccgtcact ggcaagatac cagacagtta
     9121 cctggtctgc agtctctgcc tctttaagtt catatggtaa tgatactgtg accgaacctc
     9181 taaaattcgt tattactttt cctcctgaag aaactgtcaa agtgaagacc tgcttaccgg
     9241 gaaaattatc ctgataattc gaagagacat ccttaatctc aaatctcaca tgacctgatg
     9301 tctgttcatc tattcctttt agagcatcag tgtcaaatat gagttttgca aatttatccc
     9361 cattaactgt aagagttttt cttttcaata ttagctcatc aagatttgat gctatacagc
     9421 tgtcgccgtt atgaattata gtaacaagtt ctggtggagt atatgtgttt acaggaggta
     9481 catatgctgt aatagtaata ctttgattta atccttttgg tgcagtacca gcttgagctt
     9541 tataacgcac taagactgtt cctgaagaaa ggcttgtgac atcatttccg tctccattaa
     9601 cccatgttgc tccattatca gtggaatact gcatagttgc agtaattcca gaaataactc
     9661 cagttgctga acttgcagag ggttgtgtta cagaacaggc aggtgctgtt ggtcttgctg
     9721 acagtataat gctctgtgga aggctgtcag aagtagtagt catattacct acctttacaa
     9781 gagatattgc tgtaccgaac cactcactag aaattgcaat ttcaccattt gcgtttgatg
     9841 taacattacc gctatttaaa agataagctg cattagggtt taaacctgtt aaacattcgc
     9901 ttatatagtc aacaattgca ttaggtgtgt tctctcttac aatgccatat tcatttatta
     9961 ctaatatctg attttcagaa gctaatatag taccagcagc ttttgcacga acatgatatg
    10021 ttccatctgt taggcctgtt atgtcgcttc ctgtcccgct tgtccataca gtatctgttg
    10081 aaagcttata ctccatcgct gatgtgaccc ctataagttt acc
//
LOCUS       DAOSXM010000075        10013 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2111
            NODE_4261_length_10013_cov_8.800964, whole genome shotgun sequence.
ACCESSION   DAOSXM010000075 DAOSXM010000000
VERSION     DAOSXM010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864178
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 10013)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10013)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:24:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,261
            CDSs (total)                      :: 2,229
            Genes (coding)                    :: 2,218
            CDSs (with protein)               :: 2,218
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 3 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10013
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4261_length_10013_cov_8.800964"
                     /isolate="AMR_MDS_2111"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..993
                     /locus_tag="PLQ68_08800"
     CDS             <1..993
                     /locus_tag="PLQ68_08800"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HXK72790.1"
                     /translation="FSKLITGKLGITLKEANDIIWDHKLNCLPIIDDQNNLKYLVFRK
                     DYDEHKENPMELLDNEKRYMCGAGINTRDYKERVPALVEAGADVLCIDSSDGFSEWQH
                     ETIKYVKDKFDGKVLIGAGNVVDAEGFRYLADAGADFIKVGIGGGSICITREQKGIGR
                     GQATAVIDTVRARNEYFEEKGIYIPICSDGGIVYDYHITMALAMGADFVMMGRYFARF
                     DESPTKVIKLGNNYVKEYWGEGSERARNWQRYDVGTDTGIKFEEGVDSYVPYAGKLAD
                     NLNKSIAKIKNTMSSCGSKTIKELHDTARISMVSSTSIVEGGAHDVILKDKTDF"
     gene            993..3824
                     /gene="uvrA"
                     /locus_tag="PLQ68_08805"
     CDS             993..3824
                     /gene="uvrA"
                     /locus_tag="PLQ68_08805"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003519292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HXK72791.1"
                     /translation="MQKYIFVKGAKENNLKNIDVKIPKDKLVVLTGISGSGKTTLAFD
                     TIYAEGQRRYVESLSSYARQFLGLMNKPDVEYIEGLSPSISIDQKTTSHNPRSTVGTI
                     TEIYDYLRLLYARIGIPHCPQCNKEISSQSIDQIIDIVLEYPQGTKVMVMSPIIKAKK
                     GEFQKQFESLIKDGYSRVIVDNTQYDLNEDKIELDKNKRHDITIIVDRLIIRDGIRKR
                     LTESVEIALNEADGIVIINKDLTEDILFSTKLACPSCSISIDDLAPRMFSFNSPFGAC
                     EKCLGLGILLKADPDLIIPDKSISLINGAIAVAGISFEKGSMFYSVYKMLSKEYNFKM
                     DLPFNELSDEVQNILLYGTNGNNLYVEYETKTRAFSANEPFEGIVRTVERRFKETTLS
                     YMRSYYEGFMSDSACPECNGKRLKPEVLAVTVGGKNIYEVTEVAVKECIKFFNKLELS
                     ETHKMISNVLLKEINTRLKFLVDVGLDYLTLNRSAGTLSGGEAQRIRLATQIGSGLTG
                     VIYILDEPSIGLHQRDNAKLLNALKNMRDLGNTLIVVEHDMETIAEADYIIDIGPGAG
                     IHGGEVVAQGKVADLIKSERSITGKYFSKELSIKKPEKSREMNNKFLKIYGAKQNNLK
                     NIDVMIPLGLNVCVTGVSGSGKSSLINEILYKELAFVLNGKKIKPGEHDRIEGMNYLD
                     KVINIDQTPIGRTPRSNPATYIGVFDYIRDIFSLTKESKARGYKKGRFSFNVKGGRCE
                     SCNGDGIKKIEMHFLPDVYVPCEVCKGKRYNKETLEVKYNGKTIADVLQMTIEEAYYF
                     FSSIPQIENKFRVLMEVGLGYIRVGQSSTTLSGGEAQRVKLANELSKRSTGRTMYILD
                     EPTTGLHIDDVKKLIKIMDRLIEAGNSVVIIEHNLDVIKCADYIIDLGPEGGDLGGNI
                     IAEGSVDEIMDNNNSYTGQYLKKYLNSDL"
     gene            3896..6121
                     /locus_tag="PLQ68_08810"
     CDS             3896..6121
                     /locus_tag="PLQ68_08810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015925561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HXK72792.1"
                     /translation="MRLCDICKKNVAVVFVTKIQDNKHTQVGYCIPCAKKAGISPIDH
                     LIKESGISDEELGNINNQMSQFLQNLNMDDMALPNNFQESIYPQKIEAPKKTKLKDSK
                     KDDKKKFLDAFGTNLNKKAQDGKIDNIIGRNKEIDRMIQIINRRQKNNPCLIGEAGVG
                     KTAIAEGLAQRIVNEDVPAKLINKEVYLLDMTSIVAGTQFRGQFESRMRSIINEVKEL
                     GNIILVIDEIHNIISAGDAEGGMNAANILKPALSRGEIQIIGATTIDEYRKHIEKDSA
                     LERRFQPIMVDEPSVNDTIEILHGIKKYYEQYHYVTFNDEAIRQAAILSERYITDRFL
                     PDKAIDVIDEAASRANLDNEALVKLQLLKNELDEILAEKETAASADSIDDYKRAAELK
                     ENECRVREEIRKFEEISNKIEVTVYDIAAVIEMWTKVPVNKITQIESTALLDLENRLK
                     KKIIGQKQAIDSVSKAVRRSRSGIKVKKRPASFIFVGPTGVGKTYLAKILAKELFISE
                     ESMIRLDMSEYMEKHSVSKLIGSPPGYIGYDETGQLTEKVRRNPYSLLLFDEIEKAHP
                     DVFNILLQILEDGRLTDSKGRVVQFENTIIIMTSNAGTSYKNNGFGYIHEKNDASERV
                     KEALKDIFRPEFLNRVDDIVVFTSLDDIEIFEIGKLMVDEYSIDLKEKNILLKVSDEA
                     LKFIIGEKYDDKYGARPLRRSIQTNIEDVLSEMYIKEQLKDGMMVMISLEDNQLKYEC
                     I"
     gene            6123..7001
                     /locus_tag="PLQ68_08815"
     CDS             6123..7001
                     /locus_tag="PLQ68_08815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004832961.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HXK72793.1"
                     /translation="MQAYPTLSEYLKKTFNKKIVKLPIDGGFTCPNRINNKKGCLFCS
                     DTGSGEFTYKGYSISDQIELQKKRLENKKIYNRDISYIAYFQNFTNTYDSVENLRYKY
                     TQALSCKDIVGLAIATRPDCINNEILELLKEMNKLTHLWIELGLQSSNENTAELIDRG
                     YNNKVFESCVNNLQLNGTKTVAHIIFGLPNETDEDYYKTVYYVKSLGMWGIKIHSLYI
                     QKNTRLYAYYISNPFHILSRNEYTEAVSESIKILDNQLIIHRLTGDCEKSLLFEPKWS
                     LDKLKVISEINRKLSV"
     gene            complement(6993..8930)
                     /locus_tag="PLQ68_08820"
     CDS             complement(6993..8930)
                     /locus_tag="PLQ68_08820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72794.1"
                     /translation="MTKKTHKSNTSKSKTNAKPKKQISKVEYAFGKIILVLLFISVIA
                     LAIYLLLDWSLKLANKRINNSDASVDKLISSIRTNDYETAVTLYDKIKTEQNLTDDGI
                     NKISSEINSKFNSFFDKDFEYDSNEASIIRHFSMFITEEKVNSVVNQLYNTFRTTTLS
                     YENYQNTINHINDLLKDENFENIINLHKDNAEIIRTSREMYNKAKTFEQENDYLNAYE
                     SYLGVSEKDAFYYSVAQEDAFNLKGKIKSNILSTAKTFEEENDIENAYYTMQSVPDII
                     SDDNEIIEYKKYITDLYEEQNYTEYKGTVYNMFFHSLALYPEIAFSSTRGEELFNIMT
                     TRYEFIKCLNELYEHDYILIKPSDLYDLYIKDGIEYLKLKDSLLLPEGKKPLILSFDN
                     LSCMHASVGFCKKLVIDESGNLASIVTINGTDTLTYDGEHVLILHDFVKEHPDFSYNN
                     AMAVLGMSGYESLFGYNTADLNSPDVQSELDKAKQVADKLKNMGYIFANHSYYHYSNS
                     SDIPSSYTDLEWLKYDTGLWKERIEPILGDTNIYITPGGKNYSVTKYVDGDRSDPCYN
                     YLVSEGYQIILSVGRGQFYTNNIIGVSNPRFFFETSLFMDRYNIDGKSFYKEDVKLID
                     VFGFEYKDIIDPIRESYHNSY"
     gene            9107..>10013
                     /locus_tag="PLQ68_08825"
     CDS             9107..>10013
                     /locus_tag="PLQ68_08825"
                     /inference="COORDINATES: protein motif:HMM:NF013347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HXK72795.1"
                     /translation="MKCTATCLFGLESVLASELKRMNYDKISSENGRVNFESDLYGIA
                     KANINLRTAERIYIELGNFEAKSFNELFDNTEKIQFSKFIDKNGEMPVNVSSISSELR
                     SIPNCQSIVKKALIKSMSRTYKIDKFSETGAKYPINVFIYKDLVRIYLDTTGISLYKR
                     GYRTKGGEAPLKETLASAIVQLSYWNKDKILVDPFCGSGTILIEAALIAKNIAPGLFR
                     SFISEDWKIFVNDEWSKAKDYFESQITNENIEKMYGFDIDKEVIGNARINARKAKVSD
                     IIEFRVSDFKDLQPPDEKGIIICNPP"
BASE COUNT         3833 a         1238 c         1752 g         3190 t
ORIGIN      
        1 ttttcaaaac tcattactgg aaaacttgga attactctta aagaagcaaa tgatattatt
       61 tgggatcata agctgaattg tcttccaata attgatgatc agaataattt gaaatatcta
      121 gtattcagaa aagactatga tgaacacaaa gagaatccaa tggagcttct tgataacgag
      181 aaaaggtata tgtgcggagc aggtatcaac acaagagact acaaggaaag agttcctgca
      241 ttagttgaag caggtgctga tgttttatgc atagattctt ctgatggttt tagtgaatgg
      301 caacatgaaa ccataaagta tgtaaaagat aaatttgacg gaaaagtcct tataggagca
      361 ggtaatgtag ttgatgcaga aggattcaga tatttagctg atgcaggtgc tgattttata
      421 aaagtcggta tcggtggagg gagtatatgt ataacaagag agcaaaaagg tattggcaga
      481 ggccaggcaa cagcagttat tgatactgta agagccagaa atgaatattt tgaagaaaaa
      541 ggtatatata ttcccatatg ttctgatgga ggcatagtat atgattatca tataactatg
      601 gcattagcta tgggtgcgga ttttgttatg atgggtagat atttcgcaag atttgacgaa
      661 tcaccaacaa aagttataaa attaggtaac aattacgtta aggaatactg gggagaaggt
      721 tccgaacgtg caagaaattg gcaaagatat gatgttggca cagacacggg tataaaattt
      781 gaggaaggtg ttgatagtta cgtgccatat gcaggaaaac ttgcagataa cttgaacaag
      841 tcaatagcaa aaattaaaaa tactatgtca agttgtggtt ccaaaacaat aaaggaatta
      901 cacgatactg ctaggatttc aatggtttca tcgacaagta tagtcgaagg tggcgcgcat
      961 gacgtcatat taaaggataa aaccgatttt taatgcaaaa atacatattt gttaaaggtg
     1021 caaaagaaaa caaccttaag aatattgatg ttaaaatacc aaaagataag ttggtagttt
     1081 taactggaat atcaggatct ggaaaaacaa cattggcttt tgatactata tatgctgaag
     1141 gacaaagaag atatgtagaa agtttatctt cttatgcacg tcagttttta ggcttgatga
     1201 ataaaccaga tgttgaatat attgaaggac tttctccttc aatatcaatt gatcagaaaa
     1261 ctacaagtca taatccaagg tctacagtag gtacaataac tgaaatttat gactatcttc
     1321 gtcttttata tgctagaatc ggaataccac attgtccaca atgtaacaaa gagataagtt
     1381 ctcaatcaat tgatcagata attgatatcg tattagaata tccacaaggt actaaagtta
     1441 tggttatgtc acctataata aaagcaaaga aaggtgaatt tcagaaacaa tttgaatcat
     1501 taattaaaga tggatattca agggtaattg tagataatac tcaatacgat ttaaatgaag
     1561 acaaaataga attagataaa aacaaaagac atgatataac aataattgta gatagattaa
     1621 ttattagaga tggaataaga aaaagactta ctgaatcagt tgagatagca ttaaatgaag
     1681 ctgatggaat agttataata aataaagatt tgactgaaga tattttattt tctacaaaat
     1741 tagcttgtcc aagctgttcg atcagtattg atgacttagc acctagaatg ttttctttta
     1801 acagtccatt tggtgcttgt gagaaatgtt taggtttagg aattttactc aaagcagatc
     1861 ctgatttaat aattcctgat aaatcaatat cgcttattaa tggagcgatt gctgttgcag
     1921 gtatatcttt tgaaaaaggt agtatgtttt attctgtata taagatgcta tcaaaagaat
     1981 ataactttaa aatggactta ccattcaacg aactttctga tgaggttcaa aatattttac
     2041 tttacggtac aaatggaaat aatctttatg ttgaatatga aacaaaaaca agagcttttt
     2101 ctgcaaatga acctttcgaa ggtattgtaa gaacagttga aagaagattt aaagaaacta
     2161 cactttcata tatgagatca tattacgaag gttttatgag tgattctgct tgtcctgaat
     2221 gcaatggaaa aagattaaaa cctgaagtac ttgcagttac agttggcggg aaaaatattt
     2281 atgaagtaac tgaagttgcc gttaaggaat gtattaagtt ttttaataaa cttgaattgt
     2341 cagaaacaca taaaatgatt tcaaatgtac tgctaaaaga aataaataca agacttaaat
     2401 ttttagttga tgtcggatta gactatttaa ctctcaacag aagtgcaggt acattgtcag
     2461 gtggagaagc tcaaagaata aggctagcaa ctcagattgg gtcaggatta acaggagtaa
     2521 tatatattct tgatgaacct agtataggat tgcatcaaag ggataatgca aaattactaa
     2581 atgctttaaa aaatatgaga gatttaggga atacactaat tgttgttgaa catgatatgg
     2641 aaactattgc agaagctgat tatataatcg atattggtcc aggagcagga attcacggtg
     2701 gcgaagttgt agcgcaagga aaagtggctg atcttataaa atctgaaagg tcgattacgg
     2761 gtaaatattt ttcaaaagag ctatcaataa aaaaacctga aaaatcgaga gaaatgaaca
     2821 ataagttttt aaagatatat ggagctaaac agaataattt aaagaatata gatgttatga
     2881 taccattagg cctcaatgta tgtgttactg gagtatcagg ttcgggtaaa agttctttga
     2941 taaacgaaat attatataag gaactagcat ttgtactaaa tggcaaaaag ataaaaccag
     3001 gagagcatga ccgcatagaa ggaatgaatt atcttgataa agttataaat attgaccaaa
     3061 cacctattgg aagaactcca agatccaatc ctgcaacata cattggtgta tttgattata
     3121 ttcgtgatat attttctctt actaaagaat caaaagctag aggatataaa aagggaagat
     3181 ttagttttaa tgttaaaggc ggaagatgtg aatcatgtaa tggtgatgga ataaagaaaa
     3241 ttgaaatgca ctttcttcca gatgtttatg tgccatgtga agtatgtaag ggtaaaagat
     3301 ataataaaga aactctcgaa gttaaataca acggaaaaac aattgcagat gtacttcaaa
     3361 tgactattga agaggcatat tactttttct ctagtattcc tcaaatagag aataaattcc
     3421 gtgttttaat ggaagtaggc cttggatata taagagtggg tcaatcatct acaacattat
     3481 caggcggtga agcgcaaaga gtaaaattag caaatgagtt atcgaaaaga agtacaggaa
     3541 gaacaatgta catacttgat gaaccaacaa caggattaca tatagatgat gtaaaaaaat
     3601 taattaaaat tatggacaga ttaattgaag caggcaattc tgtagtaata attgagcaca
     3661 atctagatgt aattaaatgt gcagattata ttatagattt aggtcctgaa ggcggtgatc
     3721 ttggaggtaa cataattgca gaaggttcag tagatgaaat tatggataac aataattcgt
     3781 atacaggtca atatctaaaa aaatatttaa atagtgattt atgatataat tcacatatat
     3841 ttaacatttt catgataata taatctagcc ttgaataggt aaaggagaac gaaagatgag
     3901 attatgtgat atatgtaaaa agaatgttgc ggtagtattt gtaactaaaa tacaagataa
     3961 taaacataca caggtaggtt attgtattcc ttgtgcaaaa aaggcaggaa tatcgcctat
     4021 agaccattta attaaagaat ctggaatttc ggatgaagaa ttaggtaata taaataatca
     4081 gatgagtcaa ttcttacaga atttgaatat ggatgatatg gctttaccaa ataattttca
     4141 ggaaagtata tatcctcaaa aaatagaagc accaaaaaag acaaaattaa aagattcaaa
     4201 aaaagatgat aaaaagaaat ttcttgatgc ttttggaact aatcttaata agaaagctca
     4261 agatggaaaa attgataata ttataggtag aaataaagaa attgacagaa tgatacaaat
     4321 tataaacaga agacaaaaga ataacccctg tcttataggt gaagccggtg taggtaaaac
     4381 agcaattgct gaaggacttg cacaaaggat tgttaatgag gatgttcctg caaagttaat
     4441 caataaagaa gtatatcttt tggatatgac aagcatagta gcaggaactc agtttagagg
     4501 tcaatttgaa tcaagaatga gaagtattat caatgaagta aaagagttgg gtaatattat
     4561 attagtcatt gatgaaatcc ataatataat ttcagctggt gatgctgaag gtggtatgaa
     4621 tgctgctaat attttaaaac cagcattatc aagaggtgaa atacagataa ttggagctac
     4681 tacaattgac gagtatagaa aacacattga aaaagactca gcactggaaa gacgttttca
     4741 acctataatg gttgatgaac cttctgttaa tgatactatt gagattttgc atggtattaa
     4801 aaaatattat gaacaatatc attatgtaac ttttaacgat gaggctataa gacaagcagc
     4861 aatcttatct gaaagatata taacagacag gtttctacct gataaagcta tcgatgtaat
     4921 tgatgaagct gcatctagag caaatctgga taatgaagca ttggtgaaat tacaattgtt
     4981 aaaaaatgaa cttgatgaaa tattagccga aaaggaaaca gctgcttctg cagattctat
     5041 cgatgattat aaaagagcag cggagcttaa agaaaatgaa tgtagggtac gcgaagaaat
     5101 aagaaaattt gaagaaattt ctaacaaaat tgaagtaaca gtttatgaca ttgccgctgt
     5161 aattgaaatg tggactaaag ttccggttaa taagataact caaattgaat ctactgcttt
     5221 acttgatctt gaaaatagat taaagaagaa aattattggg caaaaacaag ctattgattc
     5281 agtttcaaaa gcggtaagaa gatctagatc tggtataaaa gttaagaaaa gaccagcatc
     5341 atttattttt gttggaccta ctggcgtggg aaagacatat cttgcaaaaa ttttggcaaa
     5401 agaactattt attagtgaag aatctatgat cagattggat atgagtgaat atatggaaaa
     5461 acattctgtt tcaaaactca taggatcacc tccaggttat ataggttatg atgaaactgg
     5521 tcagttaact gaaaaagtaa gaagaaatcc atattctcta ttattatttg atgaaattga
     5581 aaaagctcat ccagatgtat ttaatatact tttacagata ttagaagatg gaagactaac
     5641 tgatagcaag ggaagagttg ttcaatttga gaatacaata attataatga catcaaatgc
     5701 tggaacatca tacaaaaaca atggatttgg ttacatacat gaaaaaaatg atgcgagtga
     5761 aagagttaaa gaagctttga aggatatttt tagacctgag tttctgaata gagtagatga
     5821 tatagttgta tttacaagtt tagatgatat tgagatattt gaaattggta aattaatggt
     5881 agatgaatat tctattgatc ttaaagaaaa aaatattctt ctaaaagtaa gtgatgaagc
     5941 cttaaagttt attatcggtg aaaaatatga cgataaatat ggtgctcgtc cgttaagaag
     6001 aagtattcag actaatattg aggatgtatt atccgaaatg tatattaaag aacaattaaa
     6061 agatggtatg atggtaatga tatctcttga ggataatcag ttaaaatacg agtgtattta
     6121 aaatgcaagc atatccaact ttatctgaat atttaaaaaa gacatttaat aaaaaaatag
     6181 taaagcttcc aattgatgga ggctttactt gccctaatag gataaataat aaaaaaggat
     6241 gcctattctg ttctgacaca ggaagcggcg aatttactta taaaggatat agtatttctg
     6301 atcaaatcga acttcagaaa aaaagactcg aaaacaaaaa aatatataat agagatattt
     6361 cttacattgc atattttcag aattttacaa acacatatga ttctgttgaa aatttaagat
     6421 ataagtatac tcaagcatta tcttgtaaag atattgtagg tttggctata gcaacaagac
     6481 ctgattgcat aaataatgaa atattagaac tgctaaaaga aatgaataaa ttaactcatt
     6541 tgtggattga gttaggattg caaagttcaa atgaaaatac tgcagaatta attgatagag
     6601 gttataataa taaagtattt gaatcatgtg taaataattt acagttaaat ggtacaaaaa
     6661 cagttgctca tataatattt ggtttgccaa atgaaacaga tgaagattat tataaaactg
     6721 tttactatgt gaaaagtctt ggaatgtggg gtataaaaat tcattcatta tatatacaaa
     6781 agaacactag attgtatgct tattacatta gtaatccatt tcatatttta tcaagaaatg
     6841 aatatactga agctgtttct gaatcaatca aaatacttga taatcaatta ataatacata
     6901 gattgacagg ggactgcgaa aaatctttat tatttgaacc taaatggtct ctagacaaat
     6961 taaaagttat tagtgaaata aatagaaagc tatcagtatg aattatgata tgattcccta
     7021 atagggtcaa ttatatcttt atactcaaaa ccgaaaacat caattaactt aacatcttct
     7081 ttatagaaac ttttcccatc aatattatat ctatccataa aaagcgaagt ctcaaagaag
     7141 aatcttggat ttgatactcc tataatatta tttgtataaa actgtcctct tccaacactt
     7201 aatattattt gatatccttc agacacaaga taattgtaac aaggatctga tctgtctcca
     7261 tctacgtatt ttgttactga ataatttttt cctccaggag taatatatat attagtatct
     7321 ccaagaattg gctcaatacg ttctttccat aagccagtat catattttag ccattccaaa
     7381 tctgtatatg aagaaggtat atcagaacta tttgaataat gataatatga atggtttgca
     7441 aagatgtaac ccatattttt taatttatct gctacttgtt ttgctttatc aagctcactt
     7501 tgaacatctg gagaatttaa atcagcagta ttataaccaa aaaggctttc atacccagac
     7561 atacctaaaa ctgccatagc attattatac gaaaaatcag gatgttcctt aacgaagtca
     7621 tgtaatataa gcacatgctc gccatcatat gtcaaagtat cagttccatt aatagtaaca
     7681 attgatgcaa gatttcctga ctcatcaata acgagctttt tgcaaaatcc aactgatgca
     7741 tgcatacagg aaagattgtc aaaagaaagt attagtggtt tttttccttc aggtaataat
     7801 aaagagtctt ttaatttcaa atattcaata ccgtctttaa tatataaatc atataaatca
     7861 gaaggtttta tcaagatata atcatgttcg taaagctcgt taaggcattt aatgaactca
     7921 tatcgcgtag tcataatatt aaataactct tcacctcttg tagaggaaaa agctatttca
     7981 ggatataatg ccaaagaatg aaaaaacata ttataaacag tacctttata ttctgtgtaa
     8041 ttctgttctt cataaagatc agtaatatat tttttatatt caattatttc attatcatca
     8101 gaaattatat ccggaactga ctgcattgtg taatatgcat tttcaatatc gttttcttct
     8161 tcaaaggttt ttgctgtact tagaatattg cttttaattt ttcctttaag attaaatgca
     8221 tcttcctgtg caactgagta atagaatgcg tctttttcag aaacccctaa ataactttca
     8281 tatgcattta aataatcatt ttcctgttca aaggtttttg ctttattata catttctctt
     8341 gatgttctta ttatttcagc attatcctta tgtaaattta taatattttc aaaattttca
     8401 tcctttaaca ggtcgtttat gtgattaata gtattttggt aattttcata tgaaagagta
     8461 gtagttctga atgtattata taattgatta actacactat tgactttttc ttcggttata
     8521 aacattgaaa agtgtcttat aatgcttgct tcattactgt catattcaaa atccttatcg
     8581 aaaaaagagt taaatttgga gtttatttca gaagaaatct tgttgattcc atcatcagta
     8641 agattttgtt cagttttaat cttatcatat aaggttacag ctgtttcata atcatttgtc
     8701 cttattgatg atatgagttt atcaactgag gcatcagaat tatttattct tttatttgct
     8761 aattttaatg accagtctag aagaagatat attgctaatg caatgaccga aataaaaagt
     8821 aaaacaagga ttattttacc aaaagcatat tcaactttag atatttgttt ttttggtttt
     8881 gcattggttt tagatttgga agtattgctc ttgtgtgttt tttttgtcat atttttttcc
     8941 tttgtaattt tcttattaga tattatcact ttttttaaaa aaaataaata gattttaatt
     9001 ttataacgca aattcaatga tgtatccttt atataataat atttgctaaa agttattata
     9061 cagtatagaa tatataatac gatttatatc gttagaagga ttaattatga aatgtacagc
     9121 aacatgcctg ttcggactag agtctgtatt agcatcagaa ctcaaaagaa tgaattacga
     9181 taaaatatct tctgaaaatg gaagagttaa ctttgaatca gatttatacg gaatcgccaa
     9241 agcgaatata aatctaagaa ctgcagaaag aatatatatt gaattaggta attttgaagc
     9301 taaatcattt aatgaattat ttgataacac ggaaaaaata caatttagta aatttattga
     9361 taaaaacgga gagatgcctg ttaatgtatc ttccatttca tcagaattaa gaagcattcc
     9421 aaattgccag tctatagtta aaaaagcgct tataaaatca atgtcaagaa catataaaat
     9481 agataaattt tctgaaactg gtgctaaata tccgattaat gtttttattt acaaagattt
     9541 agttagaatc tatctagata caacaggaat ttctctgtat aaaagaggat acagaactaa
     9601 gggtggtgaa gcaccactta aagaaacatt ggcaagtgct atagtacagc tttcatattg
     9661 gaataaagat aagatacttg ttgacccatt ctgtggatca ggtactattc ttatcgaagc
     9721 ggcactaatt gcaaaaaaca tcgcaccagg gctttttaga tcttttataa gtgaagactg
     9781 gaagatattt gtcaatgatg agtggagtaa agcaaaagac tacttcgaat cacaaataac
     9841 aaatgagaat attgaaaaaa tgtatggttt tgacattgat aaagaagtta ttggtaatgc
     9901 aagaattaat gcacgtaaag caaaagtttc agacataata gaattcagag taagtgactt
     9961 taaagattta caaccaccag atgaaaaagg aattataatt tgtaatcctc cat
//
LOCUS       DAOSXM010000076         9906 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2111
            NODE_4327_length_9906_cov_9.542686, whole genome shotgun sequence.
ACCESSION   DAOSXM010000076 DAOSXM010000000
VERSION     DAOSXM010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864178
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 9906)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9906)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:24:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,261
            CDSs (total)                      :: 2,229
            Genes (coding)                    :: 2,218
            CDSs (with protein)               :: 2,218
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 3 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9906
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4327_length_9906_cov_9.542686"
                     /isolate="AMR_MDS_2111"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..575)
                     /locus_tag="PLQ68_08830"
     CDS             complement(<1..575)
                     /locus_tag="PLQ68_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006312521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HXK72796.1"
                     /translation="MADKRDYYEVLGVNRNATDDELKKAYRVLAKKYHPDINKTAESE
                     TMFKEVGEAYSILSDPEKRRQYDQFGFDGLNGSFSSGFSGGFNPFDIFNDIFGGGFSS
                     GFSSSSSRRNTPTRGRDIKEAVKITFEEAAFGTKKTLSITRNENCEKCNGTGAKDGKS
                     MKTCPQCRGTGEMRIQQNTLFGQMITATTCN"
     gene            complement(667..2511)
                     /gene="dnaK"
                     /locus_tag="PLQ68_08835"
     CDS             complement(667..2511)
                     /gene="dnaK"
                     /locus_tag="PLQ68_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HXK72797.1"
                     /translation="MAKVIGIDLGTTNSCVAVIEGGEPVVIANAEGGRTTPSVVAFSK
                     TGERLVGQVAKRQAITNPDRTIVSIKRDMGSDRRIDVDGKKYSPQEISAMILQKVKAD
                     AESYLGEKVTQAVITVPAYFSDSQRQATKDAGKIAGLDVLRIINEPTAAALAYGMDKE
                     QEQKILVFDLGGGTFDVSILDISDGVFEVLATNGNTRLGGDDFDNKVIDYLADTFKKE
                     NGIDLRNDKMALQRLKEAAEKAKIELSSVTTSNINLPFITADSSGPKHLDITLTRAKF
                     DELTADLVQKTMDPLKKAMDDAGLKPSDIHKVLLVGGSTRIPAVQDAVKKYIGKDPFK
                     GINPDECVAIGAAIQAGVLTGDVKDLLLLDVTPLSLGIETLGGVCTKLIERNTTIPTK
                     KSQVFSTAADGQTAVDIHVLQGEREMAQYNKTLGKFQLTGIAPAPRGVPQIEVTFDID
                     ANGIVNVSAKDLGTGNEQKITITASTNLNEDEINKAVNDAKAHEKEDKLKKEEIEVRN
                     NADSLIYQTEKLLKDSSDKVDASEKSKIEELIENLKTTLKGTDIEQIKSSTESLQQEV
                     YKFSEQMYKKTGSAPGQEDPNAHTDQNQDTSKDDNVYDADYEVVDDDK"
     gene            complement(2566..3105)
                     /locus_tag="PLQ68_08840"
     CDS             complement(2566..3105)
                     /locus_tag="PLQ68_08840"
                     /inference="COORDINATES: protein motif:HMM:NF013212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HXK72798.1"
                     /translation="MAKSKNESKNVKNEDKGVEIPDISPGQEVKEQTEECTVDLDKQN
                     QELTECYKRLAAEYDNYRKRTIKEKEEIYDTCVIDITEKFLPVLDNLNLALANSKDVK
                     DEVLKGILMIHKQFIDTLEKLEIKEIDCLNQQFDPNLHDAVMTVDDDEKSGTVVEVFK
                     KGYIYKEKVVRHSMVKVAK"
     gene            complement(3105..4130)
                     /gene="hrcA"
                     /locus_tag="PLQ68_08845"
     CDS             complement(3105..4130)
                     /gene="hrcA"
                     /locus_tag="PLQ68_08845"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HXK72799.1"
                     /translation="MDKRTADILLVIIEEYVNSADPVGSKSITDKYSLGISSATVRNE
                     MHQLEKEGYITSLHTSSGRIPTDKGYRFYVDYLTEFIKQREIEEKIYRLLKRVEIEDA
                     LDKIFSRLFSNNPYTSLGVLKRNETRIDTFHILSVAKDTYLLVLILKNGVAAHKLLKE
                     QLSDNVKVNILSEYLNEKLYGMTLRDIQNTSFSFIDKFDRDNKAFFIKIVSEIMEIMN
                     QYEENEIFLKGKENLLSFPDFKDISEVKNIFDAFNREYELSEFLLYSSDDPFSVHIGN
                     EINNENLNKLSIAKHTYDLGSEGKITFGIAGPTRMNYKKVMFDLNNIENILGNMVSDK
                     TEKKEVR"
     gene            complement(4257..5129)
                     /locus_tag="PLQ68_08850"
     CDS             complement(4257..5129)
                     /locus_tag="PLQ68_08850"
                     /inference="COORDINATES: protein motif:HMM:NF024587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase family protein"
                     /protein_id="HXK72800.1"
                     /translation="MHITITGMLGSGKSTICRIIAEEKGYEVYSTGKIQRQVASQKGV
                     TTLELNKMMTEYPELDSIIDNETVRIAKENEDRTIIFDSRMAWYFVPDAYNIFLTVDP
                     YISAQRVMRADRGDVEGYSDVEEAKTKLMERASTEHERFIRIYNADYWNYNNYDLILD
                     ATWHDPDTLAAIIMGHMNMSINKNKKKRMTQSVLMSPKSLYPAVLTKNEEEPIISKKD
                     WDSPHVYIIPKSHYNYIIRGHATVLQALSNKTELISVKSINMDEKSQLIIDEQSYKLF
                     EETGEFTYVSSPNV"
     gene            complement(5132..6841)
                     /locus_tag="PLQ68_08855"
     CDS             complement(5132..6841)
                     /locus_tag="PLQ68_08855"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HXK72801.1"
                     /translation="MKKFTEIVYERPDVNQLVKRFKESFKALKNSNNFEDADKAYINM
                     QNQFKFVETMFVTAKIRSDINVKDKFYEKEMRYLNSVLPRFMLLINKIGKELINSPFK
                     KDFENKYGSQMFRLIDAELKTMNWKILLLLVKEANLGQKYSKTVASAKTKFNNEESNF
                     YNLLKYMESTDRNVRKNAFTAWADMYEGISGKLDDIYEKSVNLRKKMAKRTGFKDYIE
                     MSYIKRNRFDYNISDVEKFRQSVKEYIVPQCEIIFKKRADILGIDKIRYYDEFISFKE
                     GNPVPKADKNEFLELAQKMYSELSDETGEFFDFMRKYELFDLYTKPDKHMGGYCTFLS
                     QYDAPFIFSNFNGTSADIDVLTHEAGHAFEIYTAAKTQPITDYLSSTSEINEIHSMAM
                     EHFTYPWMELFFQDQADKYRYAHLSNALCVIPYIVCVDEFQHKVYSNNNNYSSKEIRH
                     IWRDLEKQYMPWRDYDGNSFLEEGGFWMQKQHIFLYPFYYLEYSLAQICAFELYSNMK
                     KNRSSAWEDYYRLCKSGGSKGYFELLEIANLKNPLKPETVRQAVEVVIEELMLYEKGQ
                     DIN"
     gene            complement(6851..7975)
                     /gene="hemW"
                     /locus_tag="PLQ68_08860"
     CDS             complement(6851..7975)
                     /gene="hemW"
                     /locus_tag="PLQ68_08860"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HXK72802.1"
                     /translation="MEKKLRIYIHIPFCKSKCNYCDFNSFAGMDHMFKPYFKSLISEI
                     ALYANELRGRRIDSIFIGGGTPSHVNYEYIGSILDCFKFEKDCEVTIEVNPGAIAFHN
                     FMQYKKMGINRISFGIQSFSDRVLMYMGRIHNRASAINNIHECKAAGFDNINADLIFG
                     YPKQTYEHYDESLNIIKSLEIPHVSCYSLSIEENTLLYNMVRKGVMPYPDEKLDRDMY
                     HLSIKSLQKAGIYQYEISNFAKPGFECRHNIGYWHRDEYLGFGLSAHSLYNEYRYSNT
                     SEMHEYITLLDKYEKPVKEVGKLSKEEIISEYIILRLRMNEGIHVNTFNNLFSDDFEL
                     RYSEKIDILMKKGLIDKSDDSYFLTMLGMDVANKVFVEFI"
     gene            complement(7977..9779)
                     /gene="lepA"
                     /locus_tag="PLQ68_08865"
     CDS             complement(7977..9779)
                     /gene="lepA"
                     /locus_tag="PLQ68_08865"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HXK72803.1"
                     /translation="MKKERIRNFCIIAHIDHGKSTLADRLIENTGLLTSREMYDQVLD
                     NMDIEKERGITIKSQAVRMVYKAKDNKEYILNLIDTPGHVDFSYEVSRSLAACDGAVL
                     VVDAAQGVEAQTLANVYMALDNNLEILPVINKIDLQAARPEHVIEEIENIIGIEARHA
                     PLISAKNRINIEEVLEKITQYLPPPEDHSDMPARALIFDSKFDAYKGVIIFCRVMDGI
                     IKQDDTIYLMNTEKEYDIVEIGYLKPGGFIPATELTSGEVGYITASIKNVEDTRVGDT
                     VTTIQNKAKQPLPGYKEAVSMVFCGIYPADGSKYNVLKDALMKLKLNDAALMFEPESS
                     AALGFGFRCGFLGLLHMEIIQERVEREFDLDIITTAPSVIYKVIKTNKEVLQVDNPTN
                     LPPLSEIDYMEEPIAKAIIIVPTEYVGNIMELCQDRRGNFIHMEYLDENRAKLDYEIP
                     LNEIIYDFFDALKSRSKGYASLDYEVLKYQRSKLVKMDILLNLEVIDALSFIVHESKA
                     YTRGRRMAEKLKDQIPRHQFEIPIQAAIGGKIIARETVRAYRKDVIAKCYGGDISRKK
                     KLLEKQKEGKKRMRQLGSVEVPQEAFMSVLKLDS"
BASE COUNT         2867 a         1952 c         1325 g         3762 t
ORIGIN      
        1 ttattacatg ttgtagcagt aatcatctga ccaaacagag tattctgctg tatacgcatt
       61 tctccagtac cacgacactg tggacaggtt ttcatactct ttccgtcttt tgctcctgta
      121 ccgttacact tttcgcaatt ctcatttcga gttatgctta atgtcttttt tgtaccgaaa
      181 gcagcttcct caaatgtgat ttttactgct tcttttatat ctctaccgcg agttggtgta
      241 tttcttcttg aagaagaaga tgaaaaccct gatgagaatc cgcctccaaa aatatcatta
      301 aaaatatcaa aaggattaaa tccaccagaa aatcctgatg aaaatgaacc attgaggcca
      361 tcaaatccaa actggtcata ctgccttctt ttttccggat cacttaaaat gctatatgct
      421 tcaccaactt ctttgaacat ggtttcagat tcagcagttt tattaatatc cggatgatat
      481 ttcttagcta atactctata tgcttttttt aactcatcat cggttgcgtt gcggttaacg
      541 cctaatactt cataataatc tcttttatct gccacaagta acgccccttg tcaatatttt
      601 ttcatactca ggcggcgatt tttcaatcac cgcctgagcg accgattcat tatatactat
      661 cttattttat ttgtcatcgt caacaacttc ataatctgca tcataaacgt tatcatcttt
      721 tgaagtgtcc tgattctggt cagtatgtgc gtttggatct tcttgtcctg gagcgctacc
      781 tgtcttctta tacatctgct cagagaattt atatacttcc tgctgtaagg attcagtaga
      841 agatttaatc tgttcaatat ctgttccctt aagagttgtt tttaaattct cgataagctc
      901 ttcaattttt gatttttctg aagcatcaac tttgtcagag ctgtctttaa gtaatttttc
      961 tgtctgataa attaaagaat cagcattatt tctaacttca atttcctctt tcttgagttt
     1021 atcttctttc tcatgagctt tagcatcatt aacagcttta ttgatttcat cctcgttaag
     1081 gttagttgag gcagtaatag taattttctg ctcatttcct gttcctaaat cttttgctga
     1141 aacatttact ataccgtttg catcgatatc aaaagtaact tcaatctgag gtactcctct
     1201 tggagctgga gctattccag taagctggaa ttttcccagt gtcttgttat attgagccat
     1261 ttctctttca ccttgaagta catgaatatc aacggctgtc tgaccgtctg cagctgttga
     1321 gaatacttgt gacttcttgg taggtattgt tgtattcctt tcaataagtt ttgtgcatac
     1381 cccacctaga gtctcaatac ccaatgatag tggtgttaca tctaataata gaagatcttt
     1441 aacatcacca gttaatacac cagcctgtat tgcagcaccg atagctacgc attcgtcagg
     1501 atttatacct ttgaatgggt cttttccaat atacttttta acagcatcct gcactgcagg
     1561 tattcttgta gaacctccaa ctaaaagaac tttatgaata tcagaaggtt taagtcctgc
     1621 atcatccata gctttcttca agggatccat tgttttttgt acaagatctg ctgtcagttc
     1681 gtcaaattta gctcttgtta atgttatatc aagatgcttt ggtcctgaag aatctgctgt
     1741 tatgaaagga aggttgatgt tagatgtcgt aacacttgaa agctcgattt ttgccttctc
     1801 tgcagcttct ttgagccttt gtagagccat cttgtcattt cttaaatcta ttccattttc
     1861 ctttttgaaa gtatcagcaa gataatcaat aaccttatta tcaaagtcat ctccaccaag
     1921 tcttgtatta ccatttgttg ctaaaacttc aaatactccg tctgatatat caagtataga
     1981 tacatcaaat gtaccaccgc caaggtcgaa tacaagtatc ttctgctctt gctctttatc
     2041 cattccatat gcaagagctg cagctgttgg ttcatttatt attcttaata catcaagtcc
     2101 tgcaatcttt cctgcatctt ttgtagcttg tctttgtgaa tcagaaaagt atgccggtac
     2161 tgttataacg gcctgcgtga ctttttcacc taaataactt tctgcatcag ctttaacttt
     2221 ttgcagaatc attgcagata tctcctgagg tgagtatttc ttgccgtcta catctattct
     2281 tctgtcggaa cccatatctc ttttaattga tactatagtt ctgtcaggat tagttattgc
     2341 ttgtcttttt gcaacctgac ctactaatct ttcgcctgtt tttgaaaatg cgactactga
     2401 tggtgttgtt ctgcctcctt cagcattagc tattactact ggttctccgc cttctataac
     2461 agctacgcat gagtttgttg ttcctaaatc tattcctatt acctttgcca ttttagtcct
     2521 cctttatttg tctttgactt tttttgactt ttttatttaa aaatattatt ttgctacctt
     2581 taccatgcta tgtcttacaa ctttttcttt ataaatatac ccttttttaa atacttcaac
     2641 tactgttcct gatttttcgt catcgtcaac agtcataact gcatcatgaa gatttgggtc
     2701 aaattgttga tttagacaat caatctcttt gatttctaat ttttcaagtg tatctataaa
     2761 ctgcttgtgt atcattaaaa tacctttaag cacttcatcc ttaacatcct tgctgtttgc
     2821 taaagcaaga ttaagattgt ctagcacagg cagaaatttt tcagttatat caataacaca
     2881 agtgtcatat atttcttctt tttctttaat tgtccttttt ctgtaattgt catattcagc
     2941 cgcaagtctt ttatagcatt cagtaagttc ctgattctgc ttatctaggt caacagtaca
     3001 ttcttccgtc tgctctttaa cttcctgacc tggcgaaata tctggaatct ctacaccttt
     3061 atcttcattc ttaacatttt tactttcatt tttactttta gccattatcg cacctccttc
     3121 ttttcagttt tatcactaac catatttcct aaaatatttt caatattatt aagatcaaac
     3181 attacttttt tgtaattcat ccttgtaggc cctgctatgc caaatgttat tttaccttct
     3241 gaacctaaat catatgtatg ttttgcaatt gaaagtttat ttaaattctc gttattaatc
     3301 tcatttccaa tatgaactga gaatgggtca tctgatgaat agagtaaaaa ctcacttaac
     3361 tcatattctc tgttaaaagc atcaaatata tttttaactt cagatatatc cttaaaatca
     3421 ggaaaagata ataaattttc tttaccctta agaaatatct cattctcttc atactggttc
     3481 attatttcca ttatctcaga tactattttt ataaaaaatg ctttattatc tctgtcaaac
     3541 ttatcaataa atgagaaact tgtgttttgt atatctctta aggtcattcc ataaagcttc
     3601 tcgtttaggt attctgataa tatgttaacc tttacattat cacttaactg ctcttttagc
     3661 aatttatgtg ctgcaacacc attttttaaa attaatacta aaagatatgt gtctttagca
     3721 actgataata tgtggaacgt atcaattcta gtttcattcc ttttcaaaac tcctaaggat
     3781 gtataaggat tatttgaaaa tagtctgctg aatatcttat ccaaagcatc ttctatttca
     3841 actcttttta aaagtctata aattttttct tcaatttctc tctgttttat aaactcagtt
     3901 aggtaatcta cataaaatct atatccttta tctgttggta tacgtcctga tgaagtatgc
     3961 aaagatgtaa tgtatccttc tttctcaagc tgatgcattt catttcttac agtagcactg
     4021 gatataccca atgagtattt atcagtaata ctcttagaac ccaccgggtc agcgctattt
     4081 acatattctt caattattac caacagtatg tcggctgtcc tcttatccat tttaccttcc
     4141 tttttagcac tcaagcatta agagtgctaa ctatactaaa aaataccatc taggtattcc
     4201 tttgtcaact atattttaat aaaatcatat gaacagacta tgaataagtg acattgtcac
     4261 acattgggac tgctcacata tgtgaattcc cctgtttctt cgaaaagttt gtatgattgc
     4321 tcatcaatta tcaattgact tttttcatcc atgtttattg attttacact tataagctct
     4381 gttttatttg aaagagcctg caaaactgta gcatgacctc ttattatgta gttataatga
     4441 gattttggaa ttatgtatac atggggtgaa tcccagtcct ttttacttat tataggttct
     4501 tcttcatttt ttgtaagcac tgcaggatat agtgattttg gtgacatcaa aaccgattga
     4561 gtcattcttt ttttcttgtt tttattaata gacatattca tgtgtcccat tattatagct
     4621 gctaaagtat caggatcatg ccaagttgca tcaagaatta aatcataatt attgtaattc
     4681 cagtaatcag cattatatat ccttataaat ctttcgtgtt cggtacttgc tctttccatt
     4741 aattttgttt ttgcttcttc cacatcagaa tatccttcaa catctcctct gtcggccctc
     4801 attactctct gagcagatat atacggatct acagtaagaa atatattgta cgcgtcagga
     4861 acaaaatacc atgccatgcg tgaatcgaat ataattgttc tatcttcatt ctctttagca
     4921 attctaactg tttcattatc tattattgaa tcaagttctg ggtattcggt catcatttta
     4981 ttcagttcaa gagtagttac ccccttttgt gatgcaactt gtctttggat ttttccagtc
     5041 gaatatactt catatccttt ttcttctgct attattctgc aaattgtaga ttttccgctt
     5101 cccagcattc cagtaatagt aatatgcatt ttcagttaat atcctgtccc ttctcatata
     5161 acatcaattc ttcaataacc acttctacag cctgtctgac agtctctggc ttaagcggat
     5221 tcttaagatt tgctatttca agtaactcga aatagccttt acttcctcca ctcttacata
     5281 atctgtagta atcctcccat gcggaagatc tgtttttctt catattagaa tataactcaa
     5341 atgcgcatat ctgagcaaga gaatattcca aataatagaa aggatataga aatatatgct
     5401 gcttctgcat ccaaaaacca ccttcttcaa gaaagctatt gccgtcataa tctctccatg
     5461 gcatatattg tttttctaaa tctctccaga tatgccttat ctcttttgat gaatagttat
     5521 tattatttga atatacctta tgttgaaact catctacaca tacaatatat ggaataacac
     5581 aaagtgcatt tgataaatgt gcatatctat atttatcagc ttgatcttga aaaaacagtt
     5641 ccatccaagg atatgtaaaa tgctccatcg ccattgagtg aatttcattt atctcgcttg
     5701 ttgatgagag ataatctgtt attggctgag tctttgctgc agtataaatt tcgaatgcat
     5761 gaccagcttc atgtgttaaa acatcaatat cagcgctcgt tccattaaaa ttcgaaaata
     5821 taaacggagc atcatactgt gacaaaaaag tacagtaacc tcccatatgt ttatcaggtt
     5881 ttgtatataa atcaaataac tcatactttc tcataaaatc aaaaaactct cctgtttcat
     5941 ctgataactc agaatacatt ttctgtgcaa gttccaaaaa ttcatttttg tcagccttgg
     6001 gaacaggatt tccttcttta aaggatataa actcatcata atatctaatc ttatcaatac
     6061 caagaatatc ggctcttttt ttaaatatta tttcacactg agggacaata tattccttta
     6121 cgctttgtct aaatttttca acatcagaaa tattgtagtc aaatcgattt ctctttatat
     6181 aactcatttc tatatagtct ttaaaaccgg ttctttttgc catctttttt cgcaaattaa
     6241 ctgatttttc ataaatatca tcaagtttgc cgcttatccc ttcgtacata tctgcccaag
     6301 ctgtaaaagc attttttcta acattcctat cagtactctc catatactta agtaagttat
     6361 agaaattaga ttcttcatta ttaaattttg tctttgctga agctacagtt ttagaatatt
     6421 tctgaccaag gtttgcttct ttaaccagca acagcaaaat tttccagttc atagttttca
     6481 attctgcatc tatcagtcta aacatctgtg atccatattt attttcaaaa tcctttttaa
     6541 acggactatt gataagctct ttgcctattt tattaataag aagcataaat cttggaagaa
     6601 cactattaag atatctcatt tctttttcat aaaatttatc tttaacatta atatccgatc
     6661 ttatcttagc tgttacaaac attgtttcaa caaatttaaa ctgattctgc atatttatat
     6721 atgctttatc tgcatcttca aaattattgg aattttttaa agctttaaaa gattctttaa
     6781 accttttaac taattggttt acatcaggcc tttcatatac aatttctgta aatttcttca
     6841 tatctccccc ttaaataaat tcaacaaaca ctttatttgc aacatccata cctaacatag
     6901 taagaaagta tgaatcatct gatttatcta ttaatccttt tttcatcaga atatcgattt
     6961 tttctgagta tcttaattca aaatcatcac taaaaagatt gttgaatgta tttacatgaa
     7021 ttccttcatt cattcttaat ctaagaataa tatactctga aattatttcc tccttactta
     7081 attttccaac ctctttaact ggtttttcat atttatccag taatgttatg tattcgtgca
     7141 tttctgaagt attgctatat ctgtattcat tatataatga atgcgctgaa agaccaaaac
     7201 ctaaatattc atctctatgc cagtatccga tattatgacg acattcaaaa ccaggtttag
     7261 caaaattaga tatttcatat tgatatatcc ctgctttttg aaggcttttg atacttaaat
     7321 gatacatatc cctgtccaat ttttcatctg gataaggcat tacacctttt cttaccatgt
     7381 tatataataa tgtattttct tcaattgaga gactataaca agatacatgc ggtatttcca
     7441 aactcttaat tatattaaga gattcatcgt aatgctcata ggtctgcttg ggataaccaa
     7501 atataaggtc agcattaata ttatcgaaac ctgccgcttt gcattcatga atattattta
     7561 ttgcagacgc ccggttatga atacgaccca tgtacattaa aactctgtct gaaaaagact
     7621 gaattccaaa acttattcga tttataccca ttttcttgta ttgcatgaaa ttatgaaatg
     7681 caatagctcc tggattaact tcaattgtaa cttcgcagtc cttctcaaat ttaaagcagt
     7741 caagaattga acctatatac tcatagttaa catgagaggg cgttccgccc cctatgaaaa
     7801 tactatcaat ccttctgcct ctcaattcat ttgcatataa tgcgatttcg gaaattagag
     7861 atttgaaata cggcttaaac atatgatcca ttcctgcaaa ggaattaaag tcacaataat
     7921 tacatttcga tttacaaaaa ggtatatgaa tatatatcct cagttttttt tccattttaa
     7981 gaatccagtt tcaatacgct catgaaggct tcttgcggaa cttcaactga accgagctgt
     8041 ctcatcctct ttttaccttc tttctgtttc tccaatagtt tcttctttct tgaaatgtcc
     8101 ccgccatagc acttagctat aacatctttt ctatatgctc ttacggtttc tcttgctata
     8161 atcttaccgc caatagcagc ttgtataggt atttcaaact gatgtcttgg tatctgatct
     8221 tttagctttt cagccattct cctgccacgt gtgtaggctt tactctcatg aacaatgaat
     8281 gataacgcat caataacttc aagattaagc agaatatcca tttttacaag cttgcttctt
     8341 tgatatttta atacttcgta atcaagtgaa gcatatcctt ttgaacgaga ctttagagca
     8401 tcaaagaaat cgtatataat ttcatttaag ggaatttcat aatccaactt ggcacgattt
     8461 tcatcaaggt attccatatg tatgaaattt cctcgcctat cctgacataa ttccattatg
     8521 ttaccaacat attctgttgg aactattatt atcgctttgg caatcggctc ttccatataa
     8581 tctatttcac tcaaaggagg cagatttgta ggattatcaa cttgcaaaac ttctttattt
     8641 gttttaatga ctttatatat aacacttgga gctgtagtta taatatcaag atcgaattcc
     8701 ctttcaacac gctcttggat tatttccata tgcaaaagtc ctaaaaaacc gcatctgaaa
     8761 ccaaaaccta aagcagcaga tgattccggt tcgaacatta atgcagcatc attaagctta
     8821 agtttcataa gagcatcttt tagaacattg tatttagaac catctgcagg ataaattcca
     8881 caaaatacca tagatactgc ctctttatag cctggaagag gttgttttgc tttattctga
     8941 atagttgtaa ctgtatcacc tactcttgta tcttctacat tttttatact ggcagttata
     9001 tatcctactt caccggaagt aagttctgta gcaggtataa atccacctgg ttttagatac
     9061 ccaatttcta caatatcata ttctttttca gtattcataa gatatatagt atcatcctgc
     9121 ttgatgatgc catccataac tctgcaaaaa attataactc ctttgtatgc atcaaatttt
     9181 gaatcaaaaa tcaatgccct tgccggcatg tcagaatgat cttcaggcgg tggcagatat
     9241 tgagttattt tttcaagcac ttcctcaatg tttattctgt tctttgctga aattagagga
     9301 gcatgtcgtg cttctattcc aattatattt tcaatttcct ctatgacatg ttcaggtctt
     9361 gcagcctgga gatcaatttt gttaataaca ggaagtattt ccagattatt atctaaagcc
     9421 atataaacat ttgctaaagt ttgcgcctca actccctgag cagcatcaac taccaagact
     9481 gcgccatcac atgcagctaa ggacctgctg acttcatacg agaaatcaac atggccagga
     9541 gtatcgatta aatttaaaat atattcttta ttatcctttg ctttatatac cattcgaaca
     9601 gcttgtgatt taatagttat tccacgttct ttttctatat ccatattatc aagcacctgg
     9661 tcatacattt ctcttgatgt aagcaggccc gtattttcta taagcctgtc agccaaagtg
     9721 cttttaccat ggtctatgtg cgcaattatg caaaaatttc ttattctttc ttttttcaac
     9781 ctatgctcct aatgcttttt attcgaaaaa gattatatat taactaccca ttctttacaa
     9841 tctgattatc acttgagttt ccgtatttta tcttgcatat aaataaaact gtaattaatc
     9901 tgtcaa
//
LOCUS       DAOSXM010000077         9822 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2111
            NODE_4381_length_9822_cov_11.526876, whole genome shotgun sequence.
ACCESSION   DAOSXM010000077 DAOSXM010000000
VERSION     DAOSXM010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864178
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 9822)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9822)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:24:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,261
            CDSs (total)                      :: 2,229
            Genes (coding)                    :: 2,218
            CDSs (with protein)               :: 2,218
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 3 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9822
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4381_length_9822_cov_11.526876"
                     /isolate="AMR_MDS_2111"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            93..809
                     /locus_tag="PLQ68_08870"
     CDS             93..809
                     /locus_tag="PLQ68_08870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006427420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HXK72804.1"
                     /translation="MNEFIVKFINDFKISFINNFITDDRWQYLTKGLFVTLQITFFAV
                     IIGIFLGFLIAIIRTTYDKTNKLKVLNAICKLYITIIRGTPVVLQLLIIYYVVFATVA
                     IDKVLAAVIAFGLNSAAYVAEIVRSGIMSVDEGQMEAGRSLGFTYGQTMWHIVLPQAF
                     KNILPALGNEFIVLLKETSVSGYIALADLTKGGEYIRSRTYDAFMPLIAVAIIYLSIV
                     MLLTHLVNKLERRMRRSDRS"
     gene            796..1530
                     /locus_tag="PLQ68_08875"
     CDS             796..1530
                     /locus_tag="PLQ68_08875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012341969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HXK72805.1"
                     /translation="MIEVKQLFKSFGPSGSVEVLKGIDQYINKGEKVVIVGPSGSGKS
                     TFLRCLNMLEVPTAGEIYIDDEMINGKKCDVNKLRMKMGMVFQHFNLFPHLTIMQNIT
                     IGPIKLKGKTKRQAEEQALNLLKRIGLSDKAYAYPSQLSGGQKQRVAIIRALAMEPEV
                     MLFDEPTSALDPEMVGEVLDLMKQLALEGMTMVVVTHEMGFAKEVGTRVLFMDDGMIV
                     EENNPKEFFSNPKNQRTKDFLSKILQ"
     gene            complement(1536..2672)
                     /locus_tag="PLQ68_08880"
     CDS             complement(1536..2672)
                     /locus_tag="PLQ68_08880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72806.1"
                     /translation="MKKILVIFLILLMVLTGCEAITGGPLGLEVIDEGQDYDQFLFRR
                     GTFTWTKYGIYTVVNNKIRYLDKDDNWNLLCFDSECEHDSVNCSAYVETSSIFTYDDQ
                     LYYVLYKDNKFTLNKMDLMGKNREQIKILDINETEPMSTFECQIANNYLVLNYRYLNE
                     ITEPGVIKITNESATYVTSLDKQSPTKKFLSGYSYVAMISDYWLFYIQSETEEGIHSL
                     KAYNFDTEKTITVFDNYNSYSKNVASIYNPEPNIFYWFNIEQGFMKMDTETLEKTVIK
                     EADSRIQSGGAIYDDKYVYVTNSIPAPDNKDISDEQLGVYIYDHDGNYIDFVSTSNVK
                     FIGYAFSAMNKLYLATTSTGTLPTLFYINKDDIGTGNLQWVVKR"
     gene            2928..4043
                     /locus_tag="PLQ68_08885"
     CDS             2928..4043
                     /locus_tag="PLQ68_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009256079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72807.1"
                     /translation="MDNNTRALNILNYKDADRMPAVHFGYWKELLVEWVEKGHIKKED
                     MENVGDGNVADYRLNEKLGWDFNWHTTRRGAASLRPFFKPEVLEELPDGSRKVLTGWG
                     VIEIEKPGVVSISGECDYTLKDRKAYEELYKPKLQFTEERVNPEYFNNNDWDKIDAPI
                     GLEIGSVAGDVRNILTVMGMSYMIYDDYELLDEIVSDYAELQYQVAKYTLEISKFKFD
                     FAHYWEDICYKNGPLFSPNVLEDLCAKHYKKRNDLVKSYGIDIVSLDCDGCIDQMIPI
                     WLENGVNTMFPIEVGVWDASIAPWREKYGRELRGVGGMNKHVLSYDKQAVDKEIERLK
                     PLIALGGYIPCPDHRLPPGTKFELVQYYTEKIKTVKI"
     gene            4052..5821
                     /locus_tag="PLQ68_08890"
     CDS             4052..5821
                     /locus_tag="PLQ68_08890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012389694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HXK72808.1"
                     /translation="MDGIPLNDYPRPQMVRNGYLSLNGTWKCCITKHSLKADSKRNIT
                     VDTVYEGDIVVPFSPEAKYSGVNHILMPDEAITYNLSFDIQEGFLNEITLIHFDAVDY
                     ICEVYLNGNLLTRNEGGYLPFEVDVSSIIKEGTNRLDVIVTDPTDTLSQPRGKQKLKN
                     GGIFYTPISGIWQSVWLETVPRGYVKSIKIDTDIDEKTVTVHADTELDEFFVSVIDKQ
                     DVVYQGLCDKDKKIIFKMDNIRMWSPEDPYLYNLEITAGTDVVKSYFGMRKFSIVKDE
                     FGYSRLALNNDVYFHNGVLDQGYYPDSIYTPKTDEDMVKDIKLVKKLGFNMIRKHIKI
                     EPLRWYYHCDRLGILVWQDIVNGGSQYKFSTVAALPFVGINLDDTNYKAFGREDAYYK
                     QRFEDDLSKYVRHLYNCVSLCQWTLFNEGWGQFDSVRLTDVLRNLDKTRYIDSTSGWH
                     EQEDSHSDFYSRHIYFTKIAFPKAKKLSNKVMALTEFGGYAMKTPSHMYSPEKAFGYK
                     LYKDRETLQKAYEKLFKKQVIPYIKKGLSACVYTQLSDVEEEVNGLVTYDRQILKFDS
                     QRVKAVNNIVNKMNMDERVKWKE"
     gene            5809..6864
                     /locus_tag="PLQ68_08895"
     CDS             5809..6864
                     /locus_tag="PLQ68_08895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72809.1"
                     /translation="MERVIRNQYLLYPKSEICDWFKLIYQSEFGNTHMSSNAKLVQEE
                     LVKEMKTAKDTDHHKEDIGNGHYRINLGWLKNSELSVEVFNRILMLSREMSEGTFDGF
                     WKKISVLEQSIKDIPIPVNLNDFHAFEQDYFKKGCPLLNHSEIYKEEYKPSYRVVRKE
                     FAEKIDLWIMTEKLVEHAKRNGTFVNIAIDGMSTSGKTTLGQMFEKLFDCNVIHTDDF
                     FLKKNQRTYERLTQPGGNIDYERFKHEIVNNLNEPTGFIYYAYDCRTSQMSEKKAISK
                     HLNIVEGAYSMHPYFGNIYDIRIFMETDKLTQLERVLKRNGEELFDRFKNEWIPMENK
                     YHNTFKIKENCTFIYRT"
     gene            6868..7263
                     /locus_tag="PLQ68_08900"
     CDS             6868..7263
                     /locus_tag="PLQ68_08900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HXK72810.1"
                     /translation="MKTHCIRLTKGQDILTEIKRYCETNSIKASVIASSVGCVTMAAI
                     RDAGENDKHFIQENMEILSLNGTASMYRVHLHIAFSKNDLSVIGGHLLEGCIVNTTCE
                     LVLMELDDVEFRSEFDDATGYEEIDIRAV"
     gene            complement(7260..8411)
                     /locus_tag="PLQ68_08905"
     CDS             complement(7260..8411)
                     /locus_tag="PLQ68_08905"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXK72811.1"
                     /translation="MIKTALCNISDKDKIKKLWIDLFDDSENFVDWFFENRFHPKYSC
                     CTYDDDILVSAMQSVPMNLKIREISIPSTMICGVSTKKEYRNKGFMHKTFSHYMKEMN
                     DKGIILTTLKAVSIPTYHSVSHLSCTTSAYVTLSKNDADYYKNIFDNNPINKVTKTQI
                     IDVFSNINKLNECYCKFTDLYSAIALRNEKEFLLKMHDYYSDNAQCMAVIQSGIIKGY
                     CIYFKQEGIYCEEFVVTDKDTADCLIYGLLSQNKNCEKLEIKTSKKVSEYINSSNINV
                     KDQNSMGIVNVQKLLNAMFRKMHKDYVIEVEDAFIQNNNGIFLLNGSKTQKPAHLKIS
                     SSQLLRLICGYDSLDNLIRNNNVSIYDKAACDELSKMLPVCDCFIIDEY"
     gene            8562..9524
                     /locus_tag="PLQ68_08910"
     CDS             8562..9524
                     /locus_tag="PLQ68_08910"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HXK72812.1"
                     /translation="MRKVFALGELLIDFMAARRDVSLTDCDTYIKKAGGAPPNALAAL
                     CKLGGTGYLASNVGNDPFGRFLIEQCKINGIDTSMVEMDKVHFTSCAFVSLTKSGERD
                     FIFARGADEHIDFSNLNMDILSKCEIFHFGAATGLLGGKTYQAYMKALDMAVGKDKFI
                     VFDPNYRQDFWKTRTDEFIEKSRKMIAKSHVIKTSEEEARLISGKTDLEEAANELLNF
                     GTKIVLITLSDKGCLVAKKEYMKTVPSIKVNCVDSTGAGDAFIGAFLYRLTMLDDLNE
                     IFNDCNLLEKIVLFSNKAGAICCTKLGAIEGMPTRQDIDIYMSK"
BASE COUNT         3375 a         1454 c         1887 g         3106 t
ORIGIN      
        1 atagtaaggt ctaaataatt aagattcaga cagataagct tttaagtgat aatttcactt
       61 aaggcttatc tgtttattta agcaggtaaa tcatgaatga attcatagtg aagtttataa
      121 acgattttaa aataagcttc ataaacaact ttataacaga cgacaggtgg cagtatctta
      181 caaaaggcct tttcgtgact ttgcagataa ctttctttgc agtcattata gggatatttt
      241 taggattttt aatagccatt attcgtacta cttatgataa gaccaataaa ctgaaagtac
      301 ttaatgcaat ctgtaaacta tacataacca taataagagg aactcctgtc gtacttcagc
      361 tattaatcat atattatgtc gtatttgcta ctgtagctat tgataaagta cttgcggcag
      421 taattgcttt cggactcaat tctgcagctt atgtggctga aatcgtccgt tcagggataa
      481 tgagcgttga tgaaggtcag atggaggcag gcagatcttt gggttttaca tatggacaaa
      541 cgatgtggca tatagtcttg cctcaggcat ttaaaaacat actgcctgca ctgggtaatg
      601 aatttattgt actattaaag gaaacatcag tttccggata tattgcttta gccgatctta
      661 caaaaggcgg tgaatacatc agaagccgaa catatgatgc attcatgccg ctgattgctg
      721 ttgcaatcat atatcttagc attgtaatgc ttttaacaca tcttgtgaat aagcttgaga
      781 ggaggatgcg tagaagtgat agaagttaag cagcttttta agagtttcgg accatcaggt
      841 tctgttgaag tattaaaagg tattgaccaa tacatcaata aaggtgagaa agttgtcatt
      901 gtcggaccat caggtagtgg aaaaagcaca tttttacgtt gtctgaatat gttagaagtt
      961 ccaactgcag gagagatata catagatgat gaaatgatta acggcaaaaa atgtgatgtt
     1021 aataaattaa ggatgaagat gggtatggtt tttcagcact tcaatttatt tccacatctt
     1081 accattatgc agaatataac aataggtcca attaagttaa agggtaaaac aaaaagacaa
     1141 gcagaagaac aggctttaaa ccttttaaaa aggataggtc tttctgataa agcatatgct
     1201 tatccttctc agctttcagg cggacagaaa cagcgtgtag ccatcatacg tgcgttagca
     1261 atggaaccgg aggtcatgct ttttgatgaa cctacatctg ctttggaccc ggaaatggta
     1321 ggcgaagttt tagatttaat gaaacagctc gctttagagg gtatgactat ggttgtcgtc
     1381 acccatgaaa tgggttttgc aaaagaagtc ggaacaagag tactgttcat ggatgacggc
     1441 atgatagtcg aagagaataa tccaaaagaa tttttctcaa atccgaaaaa tcagaggact
     1501 aaagatttct tatctaagat attacaataa ctaaactatc ttttaacaac ccactgtaag
     1561 ttacctgtac ctatgtcatc tttattgatg taaaagagtg taggtaacgt gccagttgat
     1621 gttgtggcaa gatatagctt attcattgca ctgaatgcat atccgataaa tttaacatta
     1681 gaagttgaaa caaaatctat gtagttgcca tcatggtcat aaatatatac tccaagctgt
     1741 tcatccgaaa tgtccttatt atctggcgca gggattgaat ttgtgacata cacatactta
     1801 tcatcataaa tagcaccgcc tgactgaatt cttgaatcag cttcttttat tacagttttt
     1861 tcgagagtct ctgtatccat tttcataaaa ccttgttcaa tattgaacca gtagaaaatg
     1921 ttcggttcag gattgtatat tgatgcaaca ttcttactgt aagaattgta attgtcaaat
     1981 acagttatgg ttttttctgt atcgaaatta tatgctttca atgaatgtat accctcttct
     2041 gtttcacttt gaatataaaa caaccagtaa tcagaaatca tagcaacata tgaataaccg
     2101 gataaaaact tttttgtagg gctttgtttg tccaaagatg taacatatgt cgcagattca
     2161 tttgtaatct taatgactcc tggttcagta atttcattta aatatctgta attaagaaca
     2221 agatagttat tggctatctg acactcaaat gtgctcattg gctctgtttc attaatatcc
     2281 agtattttga tttgttctcg gttttttccc ataagatcca ttttgttaag agtaaattta
     2341 ttatccttat acaatacata gtataactga tcgtcatatg tgaatattga tgatgtctca
     2401 acataagcgc tacagttaac agaatcatgt tcacattctg aatcaaagca tagtaagttc
     2461 cagttatcat ctttatctaa ataacgtatc ttgttattca ctacggtata gataccgtat
     2521 ttagtccagg tgaaagttcc tcttctgaac agaaattgat cgtaatcctg accttcgtct
     2581 attacctcaa gccccaatgg tccaccagta attgcttcac atccagttag aaccataagt
     2641 aatatcaaga atataactaa tatttttttc ataataattc tccattcttt gcaatcgcta
     2701 tcaaccaagt attttctgta acttaattat acaattttct atattaatgt caataattgt
     2761 tttttgtact taatatttaa acattcagtc tatcaaatgt acataatagt attaattaca
     2821 aaaaatgcaa taactatata tgacaaaggt aatctgcgat ttattttcta gtttatgttg
     2881 acttgaagta gcaataacta taaaatctgt ttgtatgcgg aggatttatg gataacaata
     2941 ctagagcact aaacatttta aattataaag atgctgacag aatgccagca gtacatttcg
     3001 gttattggaa agagcttctt gttgaatggg ttgaaaaagg tcatatcaaa aaagaagata
     3061 tggaaaatgt aggagacggt aatgtagcag actacagatt gaatgaaaag cttggatggg
     3121 attttaactg gcatactacc agaagaggtg ctgcaagctt aagacctttt tttaagccgg
     3181 aagtgctgga agaattacca gacgggtcaa gaaaagtatt aacaggatgg ggtgtaattg
     3241 aaattgaaaa acccggagtt gtatcaatat caggtgaatg tgattataca ttgaaggaca
     3301 gaaaagctta tgaggaatta tataaaccta aattacagtt tacagaagaa agagttaatc
     3361 cggaatattt taataataat gactgggata agatagatgc tcctattggt cttgaaatcg
     3421 gaagtgttgc aggagatgtc agaaacattc ttacagtaat gggcatgagt tatatgatat
     3481 atgatgatta tgaacttcta gatgagatag tatcagatta tgcagaactt cagtatcagg
     3541 ttgctaaata cactttggaa atatctaagt ttaaatttga ttttgcacac tactgggagg
     3601 acatatgtta caagaatggt ccgttgttca gtcctaatgt tcttgaagat ttatgtgcaa
     3661 aacactataa aaaaagaaat gatctggtga aaagctatgg tattgacatt gtttctttgg
     3721 actgtgacgg atgcatagac caaatgatac ctatatggtt agaaaacggt gtaaatacaa
     3781 tgttccctat cgaagtagga gtttgggatg caagtatagc tccatggaga gaaaaatacg
     3841 gcagggaatt aagaggagtc ggaggaatga acaagcatgt tctttcttat gataaacaag
     3901 ccgttgataa ggaaatagaa agactcaagc ctttaatcgc ccttggcgga tatataccat
     3961 gtcctgatca caggcttccg ccaggaacca aattcgaatt agtgcagtat tatacggaga
     4021 aaataaaaac cgttaagata taggagttga gatggacggt ataccgctga acgattatcc
     4081 taggccacaa atggttagga atggttacct gtccctaaac gggacatgga aatgctgcat
     4141 tacaaaacat tctcttaaag cagattcaaa aaggaacatt actgtggata ctgtttatga
     4201 aggggatatt gtagttcctt tttctcctga agctaaatac agcggagtaa atcacatact
     4261 tatgcctgat gaggctatta catacaatct ctcatttgat attcaagaag gttttttaaa
     4321 tgaaattact ttaattcatt ttgatgcagt tgactatatt tgtgaagtat atcttaacgg
     4381 aaatctgtta acaagaaatg agggaggata tcttcctttt gaagttgatg tatcttcaat
     4441 tataaaagag ggtacaaaca gattggatgt aatagtaacc gatccaacag atacattgag
     4501 tcagccaaga ggaaagcaga aactaaaaaa tggcggaata ttctacaccc ctatatccgg
     4561 catatggcag agcgtctggc tggagactgt tccaagaggg tatgttaaaa gcataaaaat
     4621 tgatactgac attgatgaaa aaacagttac agtacatgcc gatactgaac tggatgaatt
     4681 ttttgtttca gtaattgaca aacaggatgt agtatatcag ggactttgtg ataaggataa
     4741 aaagataata ttcaaaatgg ataatattcg aatgtggtct ccagaagatc catatttata
     4801 taatcttgaa atcacagcgg gaactgatgt tgttaaatca tattttggaa tgcggaaatt
     4861 cagtatagtt aaagatgaat tcggttattc aaggcttgct ttgaataatg atgtatattt
     4921 tcataacggt gttcttgatc aggggtatta tccagattct atatatacac ctaaaactga
     4981 tgaagatatg gttaaagaca taaagcttgt taaaaaactt ggattcaata tgattagaaa
     5041 gcatataaaa attgaaccgt taagatggta ttatcactgt gacagattag gaatactggt
     5101 atggcaggat atcgtaaatg gtggaagtca gtacaaattc agcacagttg cagctcttcc
     5161 ttttgttggc attaatctgg atgatactaa ctacaaagca tttggaagag aagatgcata
     5221 ttacaagcag agattcgaag atgacctttc taaatatgta cgccatttat ataactgtgt
     5281 ttcgttatgt cagtggactc tttttaatga agggtggggg cagtttgatt ctgtaaggct
     5341 tactgatgta ttaagaaacc tggataaaac aaggtatata gattctactt ccggatggca
     5401 tgaacaagaa gacagtcatt ctgattttta cagcagacat atatatttta ctaagatagc
     5461 ttttccaaaa gcgaaaaagt taagtaataa ggtaatggca ttaacagaat ttggcggtta
     5521 tgctatgaaa acaccttcac atatgtattc gccagaaaaa gcatttggat ataaattata
     5581 taaagacaga gaaactttac agaaggctta tgaaaaacta ttcaaaaaac aggttattcc
     5641 atatataaaa aaaggactga gcgcttgtgt ttatacacag ctttctgatg tagaagaaga
     5701 ggtaaacggg cttgttacat atgacaggca gatattaaag tttgacagcc agagggtaaa
     5761 agctgttaac aatatagtaa ataaaatgaa catggatgaa agggtaaaat ggaaagagta
     5821 attcgcaatc aatatttact ttatccaaaa tcggaaattt gtgactggtt caagctgata
     5881 taccaaagcg aatttggaaa tactcatatg tcttctaatg caaagcttgt acaagaagaa
     5941 ttggttaagg aaatgaagac agccaaggac acagaccatc ataaagagga tattgggaac
     6001 gggcactata ggattaatct tggttggttg aaaaacagcg agttgtcagt tgaagttttt
     6061 aatcgtattt taatgctaag ccgtgaaatg tccgaaggta cttttgatgg cttttggaag
     6121 aaaatatctg ttttggaaca atcaattaag gatataccta ttcctgtcaa cttaaatgat
     6181 tttcatgctt ttgagcaaga ctattttaaa aaaggctgtc cattattaaa tcatagtgag
     6241 atttacaaag aagaatataa gccctcttac agagttgtaa gaaaggaatt tgcagaaaaa
     6301 atagatcttt ggattatgac agagaaatta gttgaacatg cgaaaagaaa cggaactttt
     6361 gtaaacattg caattgacgg tatgagtact tcaggcaaaa ccacgctagg gcagatgttt
     6421 gaaaagttat ttgattgcaa tgtcattcat acggatgatt tctttctaaa gaaaaatcaa
     6481 cgaacttatg aacggctcac acaacctggc ggcaacatag attatgaaag atttaaacat
     6541 gaaatagtta ataaccttaa tgaaccaact ggtttcattt attatgcata tgactgcagg
     6601 acttcacaga tgagcgagaa gaaagcaata tcaaaacacc tcaatatagt agaaggcgca
     6661 tacagcatgc atccgtattt tggaaatata tatgatatca gaatatttat ggaaaccgat
     6721 aagttgactc agcttgaaag agtgctaaaa agaaacgggg aagaattgtt tgaccgtttt
     6781 aagaatgaat ggattcccat ggaaaataaa tatcataata cattcaagat taaagaaaat
     6841 tgtacattta tctataggac atagaacatg aaaacacatt gtataaggct gactaaaggt
     6901 caggatatat taactgaaat caaaagatac tgtgagacaa acagtatcaa agcaagtgta
     6961 atagcaagct cagtcggttg tgtaacaatg gctgcaatta gagatgcagg tgagaacgat
     7021 aaacacttta ttcaggaaaa tatggagatt ttgtctctta atggaaccgc atctatgtac
     7081 agagtacacc ttcatattgc tttttcgaaa aatgatttat ctgtaatagg aggacatctt
     7141 ctagagggat gtattgtaaa tactacatgt gagctcgtgc ttatggagct ggacgatgtt
     7201 gagtttagaa gtgaatttga tgatgcaaca ggatatgaag aaattgatat aagagctgtt
     7261 tagtattcgt ctattataaa acagtcacac accggcagca ttttcgataa ttcatcacat
     7321 gctgctttat catatatgga tacattatta tttcttatta aattatctaa agaatcataa
     7381 ccgcatatta atcttagtaa ctggcttgaa ctaattttta agtgagcggg cttttgcgtt
     7441 ttggaaccat tgagtaagaa gatgccattg ttattctgta taaaagcatc ttcaacttca
     7501 attacataat ctttatgcat ttttctgaac atggcattta gtaatttttg gacatttact
     7561 atacccatag aattctggtc tttgacattt atattactgc tgtttatata ttcagaaact
     7621 tttttagatg ttttaatttc tagtttttca caatttttgt tttgggataa caatccgtat
     7681 ataaggcaat ctgctgtatc tttatcagta actacaaatt cctcacaata tatgccttcc
     7741 tgtttgaaat atatgcagta tccctttatt atacccgatt gtataacagc catacattga
     7801 gcattatctg aatagtagtc atgcatttta agtaaaaatt ctttttcatt tcttagagca
     7861 atagcactgt ataggtcagt aaatttacag tagcattcat taagcttatt aatattactg
     7921 aacacatcaa taatttgtgt ttttgtaact ttgttaatgg ggttgttatc aaatatattt
     7981 ttgtaataat cagcatcatt cttagataaa gtaacataag ctgatgtggt acaggataaa
     8041 tgactgacag agtgatatgt cggtatgctg actgctttga gagttgtgag aattattcct
     8101 ttgtcattca tttccttcat gtaatgagaa aatgtcttat gcataaatcc tttatttcta
     8161 tattcctttt ttgttgaaac tccgcatatc atagttgaag gaatggatat ttcccttatc
     8221 tttaaattca taggcacaga ctgcatagca gaaactagta tgtcatcatc atatgtgcag
     8281 cagctatact taggatgaaa tctgttttca aagaaccagt ccacaaaatt ttcactatca
     8341 tcaaatagat caatccagag tttttttatc ttatctttat ctgaaatatt gcataaagct
     8401 gttttaatca tttaacattt atcctctatt atctttatta tagctaaaaa aagacaatat
     8461 gtcgttacat tcctcaataa cagcctatta agcatgcaag atggagtata attaagaagg
     8521 aggaatatga acagtcactg actgtaccat ataaataaga tatgagaaaa gtatttgctt
     8581 taggggaact gcttatagac ttcatggctg caagaagaga tgtgtcactt acagactgtg
     8641 atacctatat taaaaaggct ggaggagctc ctcccaatgc cttagcggca ttatgtaagc
     8701 taggaggcac aggttatctt gcttcaaatg tcgggaatga tccttttggc agatttctaa
     8761 tagagcaatg taaaataaat ggaattgaca cctcaatggt agaaatggat aaagtacatt
     8821 ttacctcatg tgcatttgta tccttaacaa aatcaggcga aagggatttc atatttgcaa
     8881 gaggagcaga cgaacatatc gattttagta atttaaatat ggatatactt tcaaaatgtg
     8941 aaatttttca cttcggagca gctaccggtc tgttaggcgg taaaacttat caagcatata
     9001 tgaaagcact tgatatggct gttggaaagg ataaattcat tgtttttgac cccaattaca
     9061 ggcaggattt ctggaaaaca agaacagatg aatttattga aaaaagcagg aaaatgattg
     9121 ctaaaagtca tgttataaag acgtcagaag aagaagcgag acttatatca ggtaaaactg
     9181 atttggaaga agcagccaat gagttattga attttggtac aaagattgta cttataactt
     9241 tgtctgataa aggatgctta gttgctaaaa aagagtatat gaaaacggtg cccagcatca
     9301 aagtgaactg tgttgactca acaggagcgg gagatgcttt tatcggagct tttttataca
     9361 gattaacaat gcttgatgat ttaaacgaaa tttttaatga ttgtaatctg cttgagaaaa
     9421 ttgttctgtt ttcgaataag gctggagcaa tctgctgcac taagcttggt gcaatagaag
     9481 gaatgccgac aaggcaggat atagatatct atatgagcaa gtaaaaccta ccccgaaaat
     9541 catattagcc ttatcgtatt atgtaatagt gatgctgtta tagcgccctc agaatgacag
     9601 tgctggtcag ggatcttcca atactcagac tccagggaac tctcaaggtg gaactgggca
     9661 aaactaaatt attgtataat tttatttatg tatttgatag cgaaagagat tatctttttc
     9721 gctatcattt tgatataggt attgacactt agtaattcag gtaatagaat aatgctgctg
     9781 gtattaccgg caatttgaaa attcaatata tatgctaggg ga
//
LOCUS       DAOSXM010000078         9399 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2111
            NODE_4656_length_9399_cov_10.131849, whole genome shotgun sequence.
ACCESSION   DAOSXM010000078 DAOSXM010000000
VERSION     DAOSXM010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864178
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 9399)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9399)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:24:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,261
            CDSs (total)                      :: 2,229
            Genes (coding)                    :: 2,218
            CDSs (with protein)               :: 2,218
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 3 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9399
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4656_length_9399_cov_10.131849"
                     /isolate="AMR_MDS_2111"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1129
                     /locus_tag="PLQ68_08915"
     CDS             join(<1..257,256..1129)
                     /locus_tag="PLQ68_08915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013623340.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HXK72813.1"
                     /translation="NFEPEFKAKVVLELLREEKTLNELAARYQISPVVISRWKSEFME
                     RAQEVFKKGPSTAEKELEDKQEEIEGLHRKIGQLTVEVDFLKKKNLRKSSKESERLGY
                     VERDNPDITVKRQCELLSVNRSSAYYERKARTEDKRNIRIMHLIDQIHTESPTYGYRK
                     ITDILRRDILINRKAVRRMMGKMGIITVYPKPNLSKRYHAEHVKPYLLRNLPITHPDH
                     VWGIDITYIRMSKGFMYLFVIIDWYSRCIVDYELSSTLDKGFVMSCLKRALSGKKPEI
                     INSDQGSHFTNPEYISLLESRGIRISMDGKGQALDNVRTERFFRSLKYDDIYINEYTT
                     PSELRKGINAYIRKYNTYRPHDSLKGDVPANIYRRSYAAAVA"
     gene            complement(1218..1787)
                     /gene="pgsA"
                     /locus_tag="PLQ68_08920"
     CDS             complement(1218..1787)
                     /gene="pgsA"
                     /locus_tag="PLQ68_08920"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004620390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HXK72814.1"
                     /translation="MNIANKLTFARIIMIPFFIIFAIPTPSWIGEPIFTFHQSYGTLV
                     ALIIFIIASVTDLLDGYYARKLKLITDLGKFIDPIADKILITSALILLVQRMEIPGYA
                     VVIIIARDLIVDGLRLVVSQKGEVIAANIYGKIKTNLQIVSVCAYFLAFYINLWFIPQ
                     IIFTLAIIATIYSGIIYIRSSVIFLSKNK"
     gene            complement(1788..3110)
                     /gene="rimO"
                     /locus_tag="PLQ68_08925"
     CDS             complement(1788..3110)
                     /gene="rimO"
                     /locus_tag="PLQ68_08925"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HXK72815.1"
                     /translation="MTKVLINSLGCPKNLVDSEIIAKTLVDFGYTLTSNIDEAEIALI
                     NTCSFIEPAVKEAIDVILETSKKKNDKNSSLKKIVVTGCLVERYKEQIITSMPEVDIC
                     VGIDGYNDIAQILKSADEKVILGPKTDTSFMNKSRLLSDNLSSTYVKISEGCDNHCTY
                     CTIPSIRGKFRSRTIEDILIEVNDLCKNGIKEITLIAQDTTAYGKDIYGKPSLYLLLQ
                     NLNKVDNLKWIRMLYCYPELITDELIQTIKSCKKVLHYIDMPLQHSSDKILKLMARHD
                     SKEDYTKLINKLRIEIPDIVLRSTFIVGFPQETENDFDDLCDFLAQTKFDRVGFFEYF
                     KEDGTAAYKISGHLPKKTKTRRRIIAEKIQQNIVIEKNKDRIGKIYSIIIQGVSEDGL
                     FYTGRSYAEAYEIDPLIYVSSIEKLTPKDIVDCKIVAIDKDSLIAEVI"
     gene            complement(3091..3576)
                     /locus_tag="PLQ68_08930"
     CDS             complement(3091..3576)
                     /locus_tag="PLQ68_08930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72816.1"
                     /translation="MSNDKKIVSKEYNKGKYILTFDDSSIVEIDEEIYFKNYIYEKET
                     LAETFIEEIVFDTQLQQCYKKALAYLINGKRTKNRMKAYLLDKGFKEDVVDASLEKLM
                     FINKINDKEFIKRFLKINIKPNSEIKRDKLIAKLIYHGIEIEDAIKAVDKEIKHDESS
                     Y"
     gene            complement(3566..4591)
                     /gene="recA"
                     /locus_tag="PLQ68_08935"
     CDS             complement(3566..4591)
                     /gene="recA"
                     /locus_tag="PLQ68_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019226995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HXK72817.1"
                     /translation="MDKKKALEASLAGIEKAYGKGAVMRLGQNVGMTVESIPTGAISL
                     DIALGIGGVPRGRIIEIYGPESSGKTTVALHVIAQAQKAGGEAAFIDAEHALDPIYAR
                     KLGVNIDELIVSQPDTGEQALEICEALVRSGALDVIVIDSVAALVPKAEIDGEMGDAH
                     VGLQARLMSQALRKLTSVVSKSKTTAIFINQLREKVGIMFGNPEVTPGGRALKFYSSI
                     RIEVRKSDNIKIGDNIIGSKTKAKIAKNKIAPPFKIAEFDIIYGHGISKEGCLVDVGV
                     ECGAIDKSGAWYSYKDQRIGQGRENAKDFLVQNPTVADEIEKVIRKAFELDKKQETKT
                     EIASDEQ"
     gene            complement(4685..6295)
                     /gene="murJ"
                     /locus_tag="PLQ68_08940"
     CDS             complement(4685..6295)
                     /gene="murJ"
                     /locus_tag="PLQ68_08940"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HXK72818.1"
                     /translation="MKESRFKSTVFLIMTSVLLSRVLGFVRTALIPIRMGGLSKVTDA
                     YNAAFQIPDLMYSMLIGGAIASSLIPVLAGYVAKGEEKKGWRSISSFMNLISIIMFVV
                     VILGVIFAEPLVNMLVPGYDEQTILLTVQLTKTLMPIAFFMMLAGLCNGIMNSYNEFA
                     VPAFGPCLYNLASIISIAVFSNNDPADDYGVNKVVLGIVICAGLYFILQFAFSFKHFK
                     HNYQPVIETRESDFKLLIVLAVPSLLTSMLVQVSLMVSRHFTAFFDVGSVTALELVNK
                     TWQMPLGIIAQAIGIAMLPSLSAVYENDKVKEVIFKINSSLRLVLFLSIPSAVGLSVL
                     SKPLIQVLFNFGTIDNKGIELASMLLIGYSLGLVMQCIITIMYRVYFSTKNSIIPFWG
                     SLIGVVFNYIIAFTLLNTTDIGIFSIPLAFSFSSVLTLVFLSLLFKTRIKSYSFRGNI
                     PIIFKSILSALIMGIVIFILERFVMPSIFQADITEIRKINQFIWILIEILIGVIVFMG
                     SAFMLKADQARDIVALGKEKIRSIFGKY"
     gene            complement(6299..7567)
                     /locus_tag="PLQ68_08945"
     CDS             complement(6299..7567)
                     /locus_tag="PLQ68_08945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004463509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HXK72819.1"
                     /translation="MKAEIIAVGTELLMGQIDNTNASYISKRLPECGVFVLYHSVVGD
                     NKTRLSECIKIALKRTDVIILTGGLGPTQDDLTKETVSDVLGLELIFQEELMNNIQLY
                     FSKLKYDKKRVVMTSNNEKQAFYPKDSKILPNRNGTAAGCIISKVVDDVEKTIVLLPG
                     PPWEMQPMFDEYVMSYFLSKNNEGLKSVYLRLFGIGESAMESLISDVVTNQTNPTIAP
                     YAKKGEVMLRVTAKYDKKKNNADAILKPVIDKLNERLSQYIYAYEDLELKDVTSKLLL
                     EKNITVSFAESCTGGMLSEMITDISGISKVYNGSVVAYSNEIKSSILSVKKSTLDKYG
                     AVSSQTAIEMAKGVRQIFNSEVGVSITGIAGPSGGTDKKPVGRVYIGLSTNDKDEFHE
                     YTFSGNREKIRYYSCIHAMNILRKYALLKG"
     gene            complement(7567..7980)
                     /locus_tag="PLQ68_08950"
     CDS             complement(7567..7980)
                     /locus_tag="PLQ68_08950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012033792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HXK72820.1"
                     /translation="MLVKQISIFLENKEGRLAELTSILAKAEININALTIADTADFGI
                     LRIIVDDADKTETLLKKEGFSVSITDVLALDIDNKPGGIAKPLNMLHENGISVEYIYA
                     FLAKKDNTAHVIFKVNDVDLAKKVLSKNGYRNTAK"
     gene            complement(7991..9289)
                     /locus_tag="PLQ68_08955"
     CDS             complement(7991..9289)
                     /locus_tag="PLQ68_08955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013412210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HXK72821.1"
                     /translation="MSIWDKIESASADEMRSIQDKRLVETVEYVYDRVPYYRKKMEDI
                     DLYPEDIKSCDDLYKLPFTTKDDLRDTYPYGLVAVDLDEIVRIHASSGTTGKRIVVAY
                     TKEDIDTWSDVMARCFARIETDSKDIVHVAYGYGLFTGGLGAHYGAEKLGATVIPISG
                     GNSRRQVEFLTDLKPTVIACTPSYMLHLYEVMIDAGYTLDDISLKAGIFGAEPWSENM
                     RKEIERLLKIKAYDIYGLSEITGPGVASECIAQNGLHINEDCYLPEIVDVIDHKPLGL
                     NEQGEIVFTTIKKKGMPLIRYRTKDITKLFKDDCICGRTLVKMERVTGRTDDMLIIRG
                     VNVFPSQIESVLLEFSQLTPNYQIIVDRINMMDVLNIHVEMNENLFTDEVKKIESLEK
                     SITGAIESVLGISAKVKLVEPKSIERSEGKAVRIIDRRKI"
BASE COUNT         3009 a         1808 c         1387 g         3195 t
ORIGIN      
        1 aattttgaac cggaatttaa agcaaaggta gtattggagc tgttgagaga ggaaaagacc
       61 ctcaatgagt tagcagccag gtatcagatc agccctgtag taataagcag gtggaaatcg
      121 gaattcatgg agagagccca ggaagtattc aagaagggcc cttcaacagc agagaaggag
      181 ctggaggata aacaggagga gattgaaggc ctccaccgca agatagggca gctgaccgta
      241 gaggtcgact tcctcaaaaa aaaatctgcg gaaatcctca aaagaaagtg aacgcctggg
      301 atacgttgag agggataacc ctgatatcac agtaaagcgg cagtgcgagc tactatcagt
      361 gaacaggagc agcgcatact atgagcgtaa agcaaggact gaggataagc ggaacatcag
      421 gataatgcac cttatcgacc aaatccatac cgagagccct acatacggat accgcaagat
      481 aaccgacata ctcaggaggg acatcctgat aaacaggaag gcagtaaggc gtatgatggg
      541 aaaaatgggg ataataacgg tatatcccaa gcctaacctg agcaaaaggt accatgccga
      601 gcatgtaaag ccatacctgc tcaggaacct tcccattacg catcctgacc atgtatgggg
      661 tatagacata acatatatca ggatgagcaa agggttcatg tacctgtttg taatcataga
      721 ctggtacagc agatgcatag tggattatga gctgtcaagc actttggaca agggattcgt
      781 gatgtcctgc ctcaagaggg cgttatcggg gaagaagcct gagataatca acagcgacca
      841 gggaagccat ttcacgaacc cggaatacat cagccttctt gaaagcagag gcatcaggat
      901 atcaatggac ggcaaaggac aggcattaga caatgtgagg acagaacgct tctttagaag
      961 cttgaagtac gatgacatat acataaacga atatacgacg ccatcagagc tgaggaaggg
     1021 gataaatgca tacatcagga aatacaacac atacaggccg cacgattctc tcaaggggga
     1081 tgtgccagcc aatatctaca gaaggtcata tgcggcagct gtcgcttgat aatactaata
     1141 acaggacaag gaaggagcaa ctatggtttc atcatttcgt gtcttgacaa tggggagcgg
     1201 tataatgttc gtaaaaatca cttattctta cttagaaata taactgatga tcgaatatat
     1261 ataattcccg agtatattgt tgcaataata gccagagtaa aaataatctg aggaataaac
     1321 cataaattaa tgtagaaagc taaaaaatat gcgcagacag aaacaatctg aagattagtt
     1381 ttaattttcc cataaatatt tgcagcaata acttcacctt tctgacttac tactaatcgt
     1441 aacccgtcga ctatgagatc ccttgcgatt attataacta cagcataacc tggtatctcc
     1501 attctttgaa ctagtaaaat taaagcgctt gtaattagta tcttatccgc tattggatca
     1561 ataaatttac ccaggtcagt aatgagcttt aattttcttg cataataacc atcaagaaga
     1621 tcagtaactg aagcgattat gaatatgatc aaggcaacca aagttccata agactgatga
     1681 aatgtaaaaa tcggttcacc aatccatgag ggagtgggta tagcaaatat tataaagaaa
     1741 ggtatcataa ttattctggc aaaagtaagt ttattagcaa tattcatcta tatgacctcc
     1801 gcaatcagtg aatccttgtc gatagctaca attttgcaat caacaatatc tttgggcgta
     1861 agcttttcta tactgctcac atatattaaa ggatctattt catatgcctc agcatatgat
     1921 cttcctgtat aaaaaagccc gtcctctgaa acaccttgaa taattatact atatatcttt
     1981 cctattctgt ctttattttt ttcaataact atattctgtt gtatcttttc agcaataata
     2041 cgccttcttg tttttgtttt tttaggaagg tgtccactta ttttatatgc agctgtacca
     2101 tcttctttaa agtattcaaa aaatccgaca cgatcaaact tagtttgagc aagaaaatca
     2161 catagatcat caaaatcatt ctcagtttct tgtggaaatc caacaatgaa ggttgaacgc
     2221 aatactatat ccggtatttc aattctaagc ttattaatta gtttggtata atcttcttta
     2281 gaatcatgtc tcgccattaa ttttaaaatt ttgtctgacg aatgttgaag tggcatatct
     2341 atataatgca atactttttt acaagatttt atagtttgaa taagttcatc agtaattaat
     2401 tccggataac agtataacat tctaatccac ttcaaattat caactttatt taagttttgc
     2461 aatagtaaat ataatgaggg ctttccatat atgtctttac cataagctgt tgtatcctgt
     2521 gcaattaagg taatttcttt gatgccattc ttacacaaat cattaacttc aattaatata
     2581 tcctcaattg ttctgcttct gaactttcct cttattgaag gaattgtaca gtaagtgcaa
     2641 tggttgtcac agccttcaga aattttgaca tatgtgcttg ataaattatc tgataataaa
     2701 cgagacttgt tcataaaact tgtatcagtc tttggtccaa gtataacttt ttcatctgca
     2761 gatttaagta tttgtgcaat atcgttgtat ccatcaatac caacacatat atctacttcc
     2821 ggcatagaag tgataatctg ttctttgtat ctttcaacaa ggcatcctgt aactactatt
     2881 ttttttaaag aactattctt atcattcttc ttcttagatg tttcaagaat tacatcaatt
     2941 gcttctttaa ctgcaggttc tataaaggag catgtattta ttaaagcaat ttctgcttca
     3001 tctatatttg atgtgagagt atatccaaaa tcaactaatg ttttagctat tatttcgcta
     3061 tcaaccagat ttttcggaca tcccaaagaa ttaataagaa ctttcgtcat gttttatctc
     3121 cttatcaact gcctttatgg catcttcaat ttcaatgcca tgatatatca gctttgcaat
     3181 aagtttatct ctttttattt cagaattagg tttaatgttt atttttagaa aacgttttat
     3241 aaactcttta tcatttattt tattaataaa cataagtttt tctaaagaag catcaacaac
     3301 atcctcttta aaacctttat caagaagata agccttcatt cggttctttg ttctttttcc
     3361 attaataaga tacgcaagag cttttttata acattgctgc aattgcgtat caaatactat
     3421 ttcttctatg aaggtttctg ctaaagtttc tttttcatat atataattct tgaaatatat
     3481 ttcttcatct atctctacta tactgctatc atcaaaagta agaatatatt tccctttatt
     3541 atactctttt gaaactattt ttttatcatt gctcatcaga tgctatttct gttttagtct
     3601 cctgtttctt gtctagttca aatgcttttc ttataacttt ttcgatttca tctgctactg
     3661 taggattctg cacaagaaaa tctttagcat tttctcttcc ttgacctatc ctttgatctt
     3721 tgtatgaata ccatgctcca gatttatcta tagctccaca ttctactcct acatcaacta
     3781 aacacccttc tttggagata ccatgtccgt aaataatatc aaattctgca atcttaaatg
     3841 gtggagctat cttattctta gcaattttcg cttttgtctt gctaccgata atattgtcac
     3901 ctattttgat attgtctgat tttcttactt caattcttat acttgagtag aattttaatg
     3961 ctcttcctcc tggtgtgact tcaggattac cgaacattat accaactttt tctctcaatt
     4021 ggtttataaa aattgcggta gtttttgatt ttgaaacaac agatgtaagt tttcttaatg
     4081 cctggctcat aagtcttgcc tgaagtccga catgcgcatc ccccatttca ccatctattt
     4141 ccgctttagg cactaatgca gcaacagaat caattactat aacatctaaa gcaccgcttc
     4201 taactaaggc ttcacaaatc tctaatgctt gttctccggt atctggttga ctgacgatta
     4261 actcatctat attaacgccg agttttcttg catatatcgg atctagtgca tgttcagcat
     4321 ctataaaagc ggcttcacct ccagcttttt gagcttgagc gataacatgc aaggcaactg
     4381 tagttttacc tgaagactca gggccatata tttcaataat ccttcctctt ggaactccac
     4441 caatccccaa agcgatgtca agtgaaattg ctcctgtcgg tatagactca acagtcattc
     4501 ccacattctg tcccagtctc ataacagctc ctttgccata tgctttttct attccggcta
     4561 atgatgcttc caatgctttc ttcttgtcca tgttttgctc cttcataatg atttgtcttt
     4621 taatttatta agattattat atatcatttg caaatttcat acaaggaata ttttttcttt
     4681 aattctaata tttaccaaat atcgacctta ttttttcttt tcctaatgca actatatcac
     4741 gagcctggtc agctttcagc ataaaggccg aacccataaa tactataaca ccaattaaaa
     4801 tttcaataag aatccaaata aactgattta tttttctaat ttcagttata tcagcttgga
     4861 aaattgatgg cattacaaat ctttctaaaa tgaaaattac aatacccata atcaatgcac
     4921 ttaaaatcga tttaaatata ataggtatat ttcctctgaa actatatgat tttattcttg
     4981 tcttaaacaa tagtgagaga aatactaacg ttaacacact tgaaaaagag aaagctaaag
     5041 gtatactgaa tatgcctata tcggtagtat taagtagtgt gaaagcaatt atataattaa
     5101 aaacaacacc tataaggctt ccccaaaatg gaattattga attttttgtt gaaaaataga
     5161 ctctatacat tattgttatt atacattgca taactaatcc caatgaatat cctattaaaa
     5221 gcatagatgc aagttcaatt cctttattat caattgttcc aaaattaaac agcacctgta
     5281 taagtggttt acttaacact gaaagtccaa ctgctgacgg tatagaaaga aacaatacca
     5341 gtcgtagtga tgaattaatt ttgaatataa cttctttaac tttatcgttt tcataaacag
     5401 ctgaaagcga tggcagcatt gcaataccaa tagcttgtgc aattattccc aaaggcatct
     5461 gccaggtctt gtttacaagt tcaagtgctg taacagaccc cacatcaaaa aaggctgtga
     5521 aatgtcttga taccataaga cttacttgga caagcattga tgtaagtaaa gatggtacag
     5581 ctaagactat tagaagttta aaatcagatt ctcttgtttc aataacaggt tggtaattgt
     5641 gcttgaaatg cttaaagcta aaagcaaatt gcagtataaa atataaacca gcacaaataa
     5701 ctatacctaa aacaacttta tttacaccat aatcatctgc aggatcattg ttgctgaaaa
     5761 cagctatact tattattgaa gcaagattgt ataaacacgg cccaaaagct ggaactgcaa
     5821 attcattata tgaattcata attccgttac ataatccagc taacatcata aaaaaggcta
     5881 ttggcataag tgtctttgta agctgaactg taagcaggat agtctgttca tcatatcctg
     5941 gaaccagcat gttcactaga ggctcagcaa aaataactcc caaaataact acaacaaaca
     6001 taattatgga gataagattc ataaatgaac ttattgaacg ccaacctttt ttttcctctc
     6061 cttttgcaac atatccagct aaaactggta ttaaagaaga tgcaatagct ccaccaataa
     6121 gcattgaata cattaaatca ggtatctgaa aggcagcatt atatgcatca gtaaccttgg
     6181 acaatcctcc cattcttata ggaattagtg ccgtcctaac aaatcccagt actctactaa
     6241 gcaatactga tgtcataatt agaaaaactg tacttttaaa ccttgattcc ttcaatattt
     6301 atcctttcaa aagagcatat tttcttaata tattcatagc atgaatgcac gagtaataac
     6361 gtattttctc cctgtttcca gaaaaggtat actcatgaaa ttcatcttta tcattcgtgc
     6421 ttaatccaat atacacacga ccgactggct ttttatctgt tccacccgaa ggtcctgcaa
     6481 ttcccgttat tgatacacca acctctgaat taaagatttg tcttactcct ttggccattt
     6541 caatagcagt ctgactgctt actgcgccat atttgtcaag agttgacttt ttaacagaaa
     6601 gtatagatga tttaatttca ttagaataag caacaacaga accgttataa acttttgata
     6661 tacctgatat atctgtaatc atttcagata acattccacc tgtacatgat tcagcaaacg
     6721 atacagttat atttttttct aacaaaagtt tcgaagtaac atccttaagt tctaaatctt
     6781 cataagcata tatgtattgt gacaatcttt cattcaactt atctattaca ggttttagaa
     6841 tagcatctgc attattcttt ttcttatcat acttagcagt tactctcaac ataacttcac
     6901 ctttttttgc ataaggagca atagttgggt tagtctgatt agttactaca tctgatatca
     6961 gagattccat tgcagactct ccaattccaa ataatctcaa atacactgat ttaagtcctt
     7021 cattgttttt acttaagaaa taactcatta catactcatc aaacataggt tgcatttccc
     7081 atggaggacc aggaagtagc actattgtct tttcaacatc atcaacaacc ttggatatta
     7141 tacaaccagc agctgtacca ttcctatttg gcaatatttt agaatctttt ggataaaatg
     7201 cctgcttttc attatttgat gtcataacaa ctcgtttttt atcatacttt aatttggaaa
     7261 agtataattg aatgttattc attaattctt cttggaaaat caattcaagt cctaaaacat
     7321 ctgatacagt ttctttagtc agatcatcct gagtgggacc taatcctcca gttagaatta
     7381 ttacatctgt tcttttgaga gcgattttga tgcactcaga aagtcttgtt ttattatctc
     7441 ccacaacaga atgataaaga acaaaaactc cgcattcagg aagtcgtttt gatatataac
     7501 tagcattagt attatctatc tgacccatta agagttcagt gcctacagca attatttcag
     7561 ctttcattat tttgcagtat tcctatatcc atttttactt aaaactttct tcgctaaatc
     7621 tacatcattt actttaaata ttacatgtgc agtattatct ttttttgcaa gaaatgcata
     7681 aatatattca actgaaatac cattttcatg aagcatattt aaaggttttg ctattccacc
     7741 gggtttattg tcaatgtcta gagctaatac atcagtaata ctaactgaaa acccttcttt
     7801 tttcagtaaa gtttctgttt tatctgcatc atcaacaata attcttaaaa ttccgaaatc
     7861 tgcagtatct gcaattgtta aagcatttat gtttatttct gccttagcta atattgatgt
     7921 tagttcagca agccttcctt ctttattttc taaaaatatc gatatctgtt tgacaagcat
     7981 tatttacccc ctaaattttc cttctatcaa ttattcgtac agcttttcct tcacttcttt
     8041 caattgattt aggctctaca agtttaacct ttgcgcttat tccaagaacg ctttcaatag
     8101 ctcctgtaat acttttttct aacgattcaa tctttttaac ttcatcagta aataaatttt
     8161 cattcatctc tacatgtata tttaaaacat ccatcatatt gattctatca actataatct
     8221 gataatttgg cgtaagctgt gaaaactcca gtaaaacgct ctcgatctga gatggaaaga
     8281 catttactcc tcttattatc aacatatcat ctgttcgtcc ggttacacgt tccattttaa
     8341 ccaaagttcg accgcatatg caatcatctt tgaaaagttt tgttatatct tttgttctat
     8401 acctgatcaa tggcatacct tttttcttta tagttgtaaa aactatttct ccttgttcat
     8461 ttaatcccaa aggtttatgg tcaataacat ctacaatttc aggaagataa cagtcttcat
     8521 ttatgtgcag tccattttga gcaatacatt ctgatgcaac tccaggacca gtaatttcgc
     8581 ttaatccgta tatatcatat gctttgattt tcagaagtct ttcgatttcc ttacgcatat
     8641 tctcactcca gggctctgct ccgaatattc ctgcttttag tgaaatatca tcaagagtat
     8701 atccagcatc aatcattact tcataaagat gaagcatata tgatggagta caagctatta
     8761 cagttggctt taaatctgtt aaaaattcaa cctgccttct actattacct ccagaaatcg
     8821 gaataactgt tgcgcctaat ttttcagcac catagtgagc tcctaaacct cctgtaaaca
     8881 aaccatatcc ataagcaaca tgaactatat ctttactatc cgtttctatt cttgcaaaac
     8941 acctagccat tacatcagac catgtgtcaa tatcttcttt ggtataagct acaactattc
     9001 ttttacctgt agttcctgat gatgcatgaa ttcttacaat ttcatcaaga tcaactgcga
     9061 caaggccata tggataagtg tcacgcaaat catcttttgt cgtaaatgga agcttatata
     9121 aatcatcaca gctttttata tcttctgggt acaagtcaat atcttccatc ttttttctgt
     9181 aatacggaac tctgtcataa acatattcaa ctgtctctac taatctctta tcctgtatag
     9241 aacgcatttc atctgctgat gcgctttcaa ttttatccca aatactcaaa tatctaacct
     9301 ccattttatt gtattttact cttatagatt caaaaaatca atgtaggaaa gttatcagat
     9361 gatatgttag tgtaaagagt tttgggaaaa ataatttat
//
LOCUS       DAOSXM010000079         9131 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2111
            NODE_4843_length_9131_cov_10.327567, whole genome shotgun sequence.
ACCESSION   DAOSXM010000079 DAOSXM010000000
VERSION     DAOSXM010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864178
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 9131)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9131)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:24:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,261
            CDSs (total)                      :: 2,229
            Genes (coding)                    :: 2,218
            CDSs (with protein)               :: 2,218
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 3 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9131
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4843_length_9131_cov_10.327567"
                     /isolate="AMR_MDS_2111"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            84..1175
                     /gene="ychF"
                     /locus_tag="PLQ68_08960"
     CDS             84..1175
                     /gene="ychF"
                     /locus_tag="PLQ68_08960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020815073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HXK72822.1"
                     /translation="MKLGIVGLPNVGKSTIFNVMTHNKAQTNNYMFSTLEPNVGIVEV
                     PDKRVDFLSQLYDTDKTTYATIEFVDIPGLVRGSNKGEGVGNKFLSSIQQVDALCHVV
                     RCFDDDDLMHVDGSVDPVRDVETINLELIFSDIEMIERRIERTKKASKSGDKSFLPVL
                     KFYEDLNEHLLEGKMAKSFHVENAEQEALLNELFLITVKPVLYVANVDEDGLSTDNDY
                     TKALEDLAEHEGSEVIKVCAKFEEDLSSLTKEEREEFLSDLGINETGLERLIKKSYKL
                     LDLMSFLTAGKKEVRAWTIKNNTTAPKAAGKIHSDFERGFIRAEVFSYDDIKRLGSYN
                     AVKEKGLVRSEGKSYIFKEGDIVLFRFNV"
     gene            1176..2408
                     /locus_tag="PLQ68_08965"
     CDS             1176..2408
                     /locus_tag="PLQ68_08965"
                     /inference="COORDINATES: protein motif:HMM:NF017452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HXK72823.1"
                     /translation="MNIVGIICEYNPLHNGHKHQIDEIKALFKNDALIVCLLSSNYVQ
                     RGEPALVDKYARADMAIESGADMVLEYPTLFCCTSAEAYAQNAIYLLNSIGIIDYISY
                     GSKSVDSNNVKSISSILANEPSQYKIMLKDELSRGLSFPKARENALSEYLENKGYNIN
                     SYRQTLQSSNDILAIEYEKALIRQKSQIKTIPIKRVGSDYNDDSLCDTFSSATAIRKT
                     LSENNGYLTKISEQIPITTKRILKEYKKYKCFANIELFKDIISYSIMEKDANELCLYP
                     LVNEGIENLLQKYVTSSSSISTFIDSCVTKRYPSTRIQRLLMHIMLGANKGLYSKYKK
                     PEYIRVLASKNKNLLSLLSNTSNLPVVTSLKKSYDSFDLNKKGLIEFENKACDIYNHV
                     VLKDKNNYKSEFSIKTKI"
     gene            complement(2472..3104)
                     /locus_tag="PLQ68_08970"
     CDS             complement(2472..3104)
                     /locus_tag="PLQ68_08970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013977160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="corrinoid protein"
                     /protein_id="HXK72824.1"
                     /translation="MIFDDISMALQKGRKKDVEALVQQAIDEKLSVKDILNKGLLAGM
                     DIVGEKFKNNELYVPDVLVAARAMNAGTALIKPLLAEAGVESIGRVVIGTVLGDLHDI
                     GKNLVKMMMEGKGFEVIDLGVNVSAEQFVQGAIDNNASIIACSALLTTTMGEMANVVK
                     VATEKGVRDKLTIMVGGAPVNESYKNSIGADLYTPDAASAAEQAAKTVLN"
     gene            complement(3181..4404)
                     /gene="tyrS"
                     /locus_tag="PLQ68_08975"
     CDS             complement(3181..4404)
                     /gene="tyrS"
                     /locus_tag="PLQ68_08975"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015312333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HXK72825.1"
                     /translation="MKVYDILEQRGFIAQSTDVQKIKHLLNNEKVTFYIGFDPTADSL
                     HVGHFLQLIVMSHLQKAGHKPIALLGGGTAMVGDPTGKTDMRRIMTREEIDYNANCFK
                     KQMSKFIDFSDDNAIMVDNYEWLSKLEYIPFLREIGMHFSVNRMLTAECFKSRMEKGL
                     SFIEFNYMLMQSYDFLLLKRKYGCKLQLGGDDQWSNIIQGVDLIRRKDSDEAFGMTFN
                     LLTTNEGKKMGKTEKGAIWLDEEKTSPFDFYQYWRNIDDAEVIKCIKLLTFVPMNQIE
                     ELSALKDKEINKAKEVLAYEVTKIVHGEQKASAAQDSSRALFSNIGQADDMPSMFVTE
                     KMLEDGFDVLQALESANFIKSNSEGRRLIQDKCVLLNNEVIEDFKFLITKGNFEDNEL
                     LLRKGKKKYFKFILK"
     gene            complement(4408..6060)
                     /locus_tag="PLQ68_08980"
     CDS             complement(4408..6060)
                     /locus_tag="PLQ68_08980"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003441978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HXK72826.1"
                     /translation="MTTLRIIPLGGLNEIGKNLTVFEYKNDILILDCGITFPQEDMPG
                     VDIVIPDMSYLIKNKHKIRGIVITHGHEDHIGAVGYLLKEVNVPVYGAKLALGLLESK
                     LNEMGLLSIAKLNVVDSNSKVSIGCFKAEFIPVNHSIPDTYAVVLTTPVGIILHTADF
                     KIDFNPVNERVADLNRFSEVGNRNVLLMLADSTNATKEGHSMSEKAVGESLDNLIGRS
                     SSRIIIASFASNINRIQQVLNLAEKYRKKVCIFGYSMRNNIKKSIELGYIKVKKGILI
                     EEFEIKNYKSKDLLLIVTGSQGETNSALSKIAFKRHQKISIDKNDLVIISASVIPGNE
                     KFLYRVINELFRRGANVIYQSLSDVHASGHAYKQELRLLITLVRPKYYMPVHGEFMHM
                     ESNAMIAMDMGINEKNIFLLENGDVMKVSSSKITVDEKITVGASFIDGSGVGDLGDDV
                     LKERMILAQDGILNISAVIDKTTRCLKAEPIITAKGFMYEKESDDLMRMIKIMLKEDF
                     DKYQKSALHISDLHNIIKKRLRSYLYQETKRNPVVLPVIIEV"
     gene            complement(6068..7969)
                     /locus_tag="PLQ68_08985"
     CDS             complement(6068..7969)
                     /locus_tag="PLQ68_08985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72827.1"
                     /translation="MNKNKDTDKSLVDEQSLSSEKEDIINVESTDKKIKADKFKTFLK
                     KNGFYLGLATLVLIMAVVLIFDANRKLKGDIPFDNTVYVIGDNSISLVKHDEEPIVLT
                     STLFKDYKDQQLGLEAHNGILMSNDEEKIMYVDELHLINNDEFSQSRLIGTLRLFDGT
                     SNIKISDEASFYYVVNDDFNTVIYKKYRVSDDNKTLINDLYKFDLITKESVLIKEDVD
                     TDIFSLSNDGKVCVFLDDYAKSYVENGEPTYSLYRYEDGKTVLVSDNVYNMEASVSTG
                     NADLNYPLINKDGKKIIYAIVNYVVMTQDEEQTNETEVNNDKIKVYPTFDMYMYDDGN
                     NELVAKSCAQLLVSYDFSQMIYIDGIVDHIDKYDEMGFGGTYYHYNLLTNEKTIVSDY
                     VFGFVPRNVIGYVDNEYIDANFYFKQYNIDDNTAHLYTYRDGKHILIDKEVSVGSDEE
                     LKLDFVTFSDDYKTVFVFKSYISKTSSLLYRYSIRSTGNVIEYMCEEKYIINKIKLTT
                     DEDYLVYNAEGRLVILSSDNTKKTIEREGVNSFDITEGGRYIYYYKETSIGAGAVYYY
                     DLHEGKESVKLVNDVNEVWNYQDNMVMFRINTDYNTMTGDLYLTDFDQFEYLSPNVYA
                     QIRTKITER"
     gene            complement(7978..9054)
                     /locus_tag="PLQ68_08990"
     CDS             complement(7978..9054)
                     /locus_tag="PLQ68_08990"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HXK72828.1"
                     /translation="MKQLKVAIIGQGRSGYSIHGSFFLKQPEKFKVVAVVDLIEKRRN
                     LAKQRYGDDVLVFENYQDLFSTTQEIDFVVNASYSHMHYSISKDLLEHGFNVLSEKPF
                     CKTANEVNDLIDTAKKNNRMLAVFQNSRYASYFLKVQEIIKSKVLGDIIQISIDFSGF
                     ARRWDWQTLQSYNGGNLYNTGPHPLDQALQLFGDGEPKVKAFMNRANTFGDAEDYVKV
                     LLYGDNKPVIDLEISSCQGYKPFTYNIQATRGALNGSMTEIDWKYFKEDECENQTLKI
                     SFDKPDSDTPAYCSESLNWHEMHWDDKGEEYGTFFSMTKRFYEMLYNHIVNGSELEVT
                     PLQVLKQIKVIEEAHKQNPLSKKY"
BASE COUNT         2996 a         1626 c         1241 g         3268 t
ORIGIN      
        1 aaatagtgaa atactttttg attgcattat gtcgatatga tataatcttt tgagaatatt
       61 cactaagagg agttaagttt tttatgaaac ttggtatagt tggactgcct aatgtaggta
      121 aaagcacaat atttaatgta atgacccata acaaagcaca aacaaacaat tatatgtttt
      181 ctacgcttga accaaatgta ggaattgttg aggttcccga taaaagagtt gattttcttt
      241 cacagcttta tgatactgat aaaacaacat acgcaactat tgaatttgtt gacataccag
      301 gcttagtaag aggctcaaat aagggtgaag gagtaggtaa taaatttctc tcaagcattc
      361 aacaggttga tgctctttgc catgtagtac gttgttttga tgatgacgat cttatgcatg
      421 ttgatggatc agttgatcct gtaagagatg ttgaaactat taatcttgaa ctcatttttt
      481 ctgacattga aatgattgaa cgcagaatag aaagaacgaa aaaagcatca aagtcgggag
      541 ataaatcatt tcttccggta ttaaaattct atgaggattt aaatgaacac cttcttgaag
      601 gtaaaatggc gaaatccttt catgtcgaaa atgcagaaca agaagcttta cttaatgaat
      661 tattcttgat aactgtaaaa ccagttctat atgttgctaa tgttgatgaa gatggattat
      721 ctactgataa tgattataca aaagcacttg aagatctggc agagcacgaa ggaagcgaag
      781 ttataaaagt atgcgctaaa ttcgaggaag acttatcctc tttaactaaa gaagagagag
      841 aagaatttct gtctgatttg ggaataaatg aaacaggtct cgaaagactt attaaaaaga
      901 gctataaact ccttgatctt atgagttttt taacagcagg caaaaaagaa gttagggctt
      961 ggaccattaa gaataacact actgctccaa aagctgcagg aaaaattcac tccgattttg
     1021 aaagaggttt tattagagct gaagtatttt catatgatga tataaaaaga ttaggctcat
     1081 ataatgcagt aaaggaaaaa ggcctagtaa gaagcgaagg taaatcatat atttttaagg
     1141 aaggagacat agtgcttttc aggtttaatg tttaaatgaa tattgtagga attatttgtg
     1201 aatataatcc attacacaat ggtcataaac atcagattga tgaaatcaag gcattattta
     1261 aaaatgatgc cttaattgtc tgcctgttaa gttcaaatta tgttcaaaga ggagaaccag
     1321 ctcttgttga taaatatgct agagcagata tggctattga aagtggagct gatatggtac
     1381 tagagtaccc tacccttttt tgctgtacaa gcgctgaagc ttacgcacaa aatgctattt
     1441 atttattaaa tagcatcggt attatagatt atataagcta tgggtcaaaa tcggtagatt
     1501 ctaataatgt taaatcgata tcttctattc ttgctaatga accatctcaa tacaaaataa
     1561 tgttaaaaga tgagttatca agaggacttt ctttcccaaa agcaagagaa aacgctcttt
     1621 ctgaatatct tgaaaataag ggttacaaca taaattcata caggcagacc ctacaatcat
     1681 caaatgatat cttagcaatt gaatatgaaa aagcattaat tcgtcagaaa tcacaaatta
     1741 agacgatacc cataaaaaga gttggttctg attataatga tgattcttta tgtgacacct
     1801 tttccagtgc tacagctata agaaagacat tatctgaaaa taatggatat ttgacaaaaa
     1861 tttctgagca aataccaata actacaaagc gaatcctaaa agaatataaa aaatataaat
     1921 gttttgcaaa tatagaatta tttaaagaca taatttccta ttctataatg gaaaaagatg
     1981 ctaatgaatt atgcctttat cctcttgtta atgaaggaat tgaaaattta ctgcaaaaat
     2041 atgttaccag cagttcttca attagcactt ttattgactc atgtgtaact aaaagatatc
     2101 cttcaacaag aattcaacgc ttactcatgc atataatgct aggtgcaaac aagggtttat
     2161 attctaaata caaaaaacca gaatatataa gggttttagc atctaagaat aaaaatctgc
     2221 tttcattatt atctaatact tcaaatctac ctgtagtgac ctctttaaaa aaatcttatg
     2281 acagttttga tttaaataaa aaaggactta ttgaatttga aaataaagca tgtgacatat
     2341 ataatcatgt cgtacttaaa gataaaaata attacaaaag tgaattttct ataaaaacaa
     2401 agatttaaca taaaaaaata aaaggatgaa taatcatcct ttttaattag ctaaatatgt
     2461 tacagttaaa tttagttcaa tactgttttc gcagcttgtt ctgcagcgct tgcagcatca
     2521 ggagtatata aatcagctcc tattgaattc ttatatgatt cgttaactgg agctccacct
     2581 accataatgg tcaatttatc tctaacgcct ttttcagtag ctacttttac aacattagcc
     2641 atctcgccca ttgttgttgt gagaagagct gaacaagcaa ttatacttgc attattatca
     2701 atagcaccct gaacaaactg ttcagcagaa acattgacgc ccaaatcaat aacttcaaac
     2761 ccttttcctt ccatcatcat cttaacaagg tttttgccta tatcatgcaa atcacctaat
     2821 actgttccga ttacaactcg gccaattgat tccacacctg cttcagcaag taatggttta
     2881 atcagtgctg taccagcatt cattgctctt gctgcaacta aaacatcagg aacgtacagt
     2941 tcgttatttt tgaatttctc gccgactata tccattcctg caagtaatcc tttgttcaag
     3001 atatccttaa cagaaagctt ttcatctata gcctgctgca ctaatgcttc aacatctttc
     3061 ttgcggcctt tctgtagtgc catcgaaata tcatcaaaaa tcatattttt accctccaat
     3121 aactttcaaa ttagtatcta ttaaaccaaa taatacaaaa tattgcaaca tcaattttga
     3181 ttatttaaga ataaatttaa aatatttctt ttttcccttc cttaataaaa gctcgttatc
     3241 ctcaaaattc cctttagtaa taagaaactt aaagtcttct ataacctcat tattcaataa
     3301 aacgcattta tcttgaataa gtcttcttcc ttcgctgttt gattttataa aatttgctga
     3361 ttcaagtgcc tgcaatacat caaacccatc ttccaacatc ttttctgtta caaacataga
     3421 aggcatatca tcagcttgtc ctatatttga gaataatgct ctggaagaat cttgagcagc
     3481 agaagccttt tgctctccat gaacaatctt agttacttca tatgccaaaa cttcttttgc
     3541 tttgtttatt tctttgtctt taagagcaga aagctcttca atctgattca ttggtacaaa
     3601 tgtaagaagt ttaatacatt ttataacttc agcatcatca atatttctcc aatactgata
     3661 gaaatcaaaa ggcgatgttt tttcttcatc aagccatata gcgccttttt cagtcttccc
     3721 cattttcttg ccttcatttg tagttagaag attaaaggtc attccaaatg cctcatctga
     3781 atcctttctc cttattaaat ctactccttg aatgatgttg ctccattgat cgtctccacc
     3841 aagctgaagt ttacaaccgt attttctttt taacaacaga aaatcataac tttgcattaa
     3901 catataatta aactcaataa aagataaacc tttttccatt ctggatttga agcattctgc
     3961 agtaagcatc ctgttaacag aaaaatgcat tccgatttct cttaaaaatg gaatatattc
     4021 aagttttgat aaccattcat aattgtcaac cataattgca ttatcatcag aaaaatcaat
     4081 aaattttgac atctgttttt taaagcaatt tgcattataa tcaatttctt ctctagtcat
     4141 aattcttctc atgtcagttt ttcctgttgg gtcaccgacc atagcggtac ctccacctaa
     4201 taatgctatt ggtttgtgac ctgctttttg aagatgactc attactatta gctgaagaaa
     4261 atgccctaca tgaaggctat cagcagtagg gtcaaatcct atataaaatg ttactttttc
     4321 attatttagc agatgcttta ttttctgcac atcagtcgac tgggcaataa atcctctttg
     4381 ttctaaaatg tcatatactt tcattaacta cacctctatt attaccggta acacaaccgg
     4441 atttcgtttg gtttcctgat acagataact gcgtaaacgt tttttgataa tgttatgcag
     4501 atcacttata tgtaaagctg atttttgata tttatcaaaa tcctctttca acattatctt
     4561 tatcattctc atcaaatcat ctgattcttt ttcgtacata aaaccctttg cagtaattat
     4621 aggttctgct tttaagcatc ttgttgtttt gtcaataact gcagaaatat tcaatatacc
     4681 atcttgtgct aatatcattc tttccttaag tacatcatct cctaaatcgc caacaccaga
     4741 cccatcaatg aaagaagctc ctactgttat cttttcatct actgtaattt tactagaaga
     4801 tactttcatt acatctccat tttcaagtaa aaagatattt ttctcattga ttcccatatc
     4861 catggcaatc attgcattgc tctccatatg cataaattcg ccatgcacag gcatatagta
     4921 cttaggacga acaagagtaa ttaaaagcct caattcctgc ttataggcgt gtcctgaagc
     4981 gtgtacatca cttaacgact ggtaaataac atttgctcct cttctaaaaa gttcatttat
     5041 tactctatat aagaatttct cattacctgg tataacagat gcagatataa taactaaatc
     5101 atttttgtca atgcttatct tctggtgtcg cttgaatgct atcttagaaa gtgcggaatt
     5161 ggtttcacct tgggatcctg ttactattaa aagcagatct tttgatttat aattctttat
     5221 ttcaaattct tcaattaaaa tacccttttt aacttttatg taccctaatt caatagattt
     5281 tttaatgttg tttctcatgc tgtaaccaaa tatacaaacc tttttacgat acttctcagc
     5341 tagatttaac acttgttgta ttctgttgat gtttgaagca aaagatgcaa taattatcct
     5401 tgaagatgat cttccaatta aattatcaag tgactctcca acagctttct cactcatgct
     5461 atgaccttct tttgttgcat ttgtactatc agcaagcatc aataaaacat tcctatttcc
     5521 aacttcggaa aatctattta agtcagcaac tctttcatta actggattga aatcaatctt
     5581 aaaatctgca gtgtgaagta ttattcctac aggtgtagtt aaaacaactg catatgtatc
     5641 tggaatacta tgatttactg gtataaattc agctttaaaa catccaattg atacttttga
     5701 gtttgaatca acaacattga gtttagcaat gctcaataat cccatttcat ttaatttaga
     5761 ttcaagcaat cctaaagcaa gttttgctcc atataccgga acattaacct cttttaacaa
     5821 ataacccact gcaccaatat gatcttcatg accatgagta ataacaatac ctctgatttt
     5881 gtgtttgttt tttattaagt aagacatatc tggtattaca atatcaacac cgggcatatc
     5941 ctcttgtgga aatgttatac cgcaatcaag tatcaaaatg tcatttttgt actcaaaaac
     6001 agtaagattc ttcccaattt catttaatcc gccaagcgga attattctta aagttgtaat
     6061 ttcgttttta cctttctgtt atctttgttc ttatttgcgc atatacattt ggtgacagat
     6121 attcaaactg atcaaaatct gtgagatata agtcacctgt cattgtattg taatccgtat
     6181 taattctgaa cattaccata ttatcctgat aattccatac ttcattaaca tcatttacta
     6241 atttgactga ttcttttcct tcatgaaggt cataataata tactgcacct gcaccaattg
     6301 aagtctcttt atagtagtaa atgtatcttc ctccttcagt tatatcaaaa gaatttacac
     6361 cttctctctc aatagtcttt ttagtattat ctgaagataa aattactaat ctgccttcag
     6421 cattatatac cagataatct tcatctgttg tcaatttaat tttgttaata atgtacttct
     6481 cttcgcacat atactcaata acatttccag tagatctaat tgaatatctg tataaaagac
     6541 tgcttgtttt gcttatgtat gatttaaata caaaaactgt tttatagtca tcagaaaatg
     6601 taacaaagtc tagctttagt tcttcatcag aaccaacaga aacttcttta tctatcagta
     6661 tatgtttccc atccctgtag gtatataaat gagctgtatt atcgtcaata ttgtattgtt
     6721 taaaatagaa atttgcatca atatactcat tatccacata tccgataaca tttcttggaa
     6781 caaatccaaa tacataatct gagacaattg tcttttcatt agttaataaa ttgtagtgat
     6841 aatatgttcc gccaaatccc atttcatcat atttgtcgat atggtcaact attccatcaa
     6901 tatatatcat ttgagaaaag tcatatgaaa ctagaagctg tgcacagctt tttgctacaa
     6961 gctcattgtt gccatcatcg tacatataca tatcaaatgt aggatatact tttatcttat
     7021 cattatttac ttctgtttca tttgtctgtt cttcgtcttg tgtcataacg acataattaa
     7081 cgatagcata tattatcttt ttaccgtcct tgtttatcaa tggataattt aaatccgcat
     7141 tacctgttga tactgaagct tccatattat atacattgtc agatacaaga acagtttttc
     7201 catcttcata tctgtataag gaatatgttg gttctccgtt ttcaacataa ctttttgcat
     7261 aatcgtcaag aaatacgcaa actttaccat cattactcaa agaaaaaatg tctgtatcta
     7321 catcttcttt aattaaaaca gattcttttg ttattaaatc aaatttatat aaatcgttga
     7381 ttagagtttt attatcatca cttaccctat actttttata tataacagta ttaaaatcat
     7441 catttactac ataataaaaa ctggcttcat cagagatttt aatgttgcta gttccatcaa
     7501 agagccttaa tgtaccaata agccttgatt gagaaaactc atcgttgttt ataagatgaa
     7561 gttcatctac atacattatt ttctcttcat cattactcat cagaattcca ttatgagctt
     7621 ctaaacctag ctgctgatct ttataatcct tgaataaagt ggatgtcaga actataggtt
     7681 cttcatcatg ttttacaagg gatatcgaat tatctccgat aacatacaca gtattatcaa
     7741 aaggaatatc tccttttagt tttcgattag catcgaaaat aagtactact gccattatta
     7801 aaactagcgt tgcaaggcca agataaaatc cattcttttt aaggaaagtt ttaaatttat
     7861 cagcttttat ttttttatct gtactttcaa cattaattat atcttctttt tctgaactta
     7921 atgattgttc atcaactaaa gacttatctg tatctttgtt tttattcata ttaactccta
     7981 atacttttta gataatggat tctgtttatg agcttcttca attactttaa tctgttttaa
     8041 tacttgtaaa ggagtaactt caagttcact tccattaact atatggttat atagcatttc
     8101 ataaaatctt tttgtcatgc taaaaaatgt tccgtactcc tcgcctttgt catcccagtg
     8161 catttcatgc cagtttaatg attcagaaca gtaagctggt gtgtcagaat ctggtttatc
     8221 gaaagatatc ttcaatgtct gattttcaca ttcatcctct ttgaaatatt tccagtctat
     8281 ctcagtcata cttccgttta gcgctcctct tgttgcctgt atattatagg taaagggttt
     8341 gtatccctgg caagatgaaa tttccaggtc tataacaggt ttattgtccc cataaagtaa
     8401 aactttaaca taatcttctg catcaccaaa tgtgtttgct ctgttcatga atgctttaac
     8461 tttaggttct ccatcaccaa aaagttgtaa tgcctgatcc aatggatgtg gtcctgtatt
     8521 gtaaagattg cctccattat atgattgtaa tgtctgccaa tcccatcttc tggcaaatcc
     8581 tgaaaaatca atacttatct gaattatatc acctaaaact tttgatttta taatctcctg
     8641 gactttcaga aaatatgatg catatcttga attttgaaat actgcaagca tcctattatt
     8701 cttttttgct gtatcaataa gatcatttac ctcatttgca gttttacaga agggtttttc
     8761 acttaataca ttaaatccat gttcaagaag atcctttgat atgctatagt gcatatggct
     8821 ataagatgca tttactacaa aatcaatttc ttgtgtagtg ctgaataggt cttggtaatt
     8881 ttcaaatact aatacatcat caccatatct ttgttttgca agatttcttc ttttttcaat
     8941 caagtctaca acagctacca ccttaaattt ctctggttgc tttaaaaaga atgaaccgtg
     9001 aattgaatat ccgcttcttc cttgtcctat tatcgctact tttaactgtt tcataatacc
     9061 ttccttcttt gtgccaagtc aaagtttata ttacaatatt taatcgtaaa attcaacaaa
     9121 ttataaagtt t
//
LOCUS       DAOSXM010000080         9100 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2111
            NODE_4866_length_9100_cov_8.971587, whole genome shotgun sequence.
ACCESSION   DAOSXM010000080 DAOSXM010000000
VERSION     DAOSXM010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864178
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 9100)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9100)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:24:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,261
            CDSs (total)                      :: 2,229
            Genes (coding)                    :: 2,218
            CDSs (with protein)               :: 2,218
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 3 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9100
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4866_length_9100_cov_8.971587"
                     /isolate="AMR_MDS_2111"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            176..1729
                     /locus_tag="PLQ68_08995"
     CDS             176..1729
                     /locus_tag="PLQ68_08995"
                     /inference="COORDINATES: protein motif:HMM:NF025925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASKHA domain-containing protein"
                     /protein_id="HXK72829.1"
                     /translation="MTVKILTDEKTYEIEFNKISAPNIYSLIKSIPQIDFIAPCGGGQ
                     RCGKCKIKLINDNSDISDSESKFLTPAERKDNIRLACFVNVSDGQIIDLRNIKAIQSI
                     MTDNRLTNSKIVVNPLITEGYGVSIDIGTTTVAASLYDLQTANRISVTSDINRQSRFG
                     SDVISRIQFASTHENLMIIQDTILNEINELITKLCKSNNLSNNQINLVYIAGNTTMLH
                     LFMGLDPTGIGVAPFTPVTLETHFFSPDDVTLKSIININNKGKIMVCASIASYVGADI
                     VAGILASGIHTSDKPCILLDVGTNGEIVLGNKDRILCCATAAGPAFEGANISCGVAGI
                     QGAINTVSYDDANTFTTIGDKSPIGICGSGLIDAIYSMLRNGIIEESGWMEDTEGFKI
                     TDNVILTQKDVREVQNAKAAIAAGLKILIKRSGYKYSEIDKLYLAGGFGTVMNVESSA
                     GIGLIPPQLKEKVIPAGNTSLTGITMLLLDKTNIETIENIRKITEYIELSQDSEFTDE
                     YVDNMFFEI"
     gene            1726..2253
                     /locus_tag="PLQ68_09000"
     CDS             1726..2253
                     /locus_tag="PLQ68_09000"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HXK72830.1"
                     /translation="MNTKKQLRIDMTQKRNLLSKEQVSALSDNLFNRFLNSDLTKYKN
                     YMLYLPIKNETDTSSLIDYLLENKKNVYVPYISDIKIVPVNINQNTEFEHDSFEIRIP
                     IEKVQLESNLLDVIFVPGLAFDKAGYRVGYGKGYYDNFLKDLSCITCAWCYDFQIVDK
                     IADIKEHDIPVNCFI"
     gene            complement(2920..4548)
                     /locus_tag="PLQ68_09005"
     CDS             complement(2920..4548)
                     /locus_tag="PLQ68_09005"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016439460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular transferase
                     activity, phosphotransferases [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HXK72831.1"
                     /translation="MNNEYIRWMSHKLKDNDLTKELKIMKSNNNLIEDAFYTQLEFGT
                     GGLRGIIGAGINRMNIYTVRKATFGYLATLKNNSKVAIAYDSRIKSLTFAKEAATVLA
                     NCGMKVYLFNKVSPTPLLSYAVRHLKCDGGIVITASHNPSNYNGYKVYDSKGCQINPD
                     KAEQIYHNMQKTDIFYEINNELSFEECLERKLITYIDDDITISYVNTVCSEAFYSGQR
                     ELKVVYTPLNGSGMECVSLALEKRGFKKINFVKEQSYPDGNFTTCPYPNPEVKEALEL
                     GIRDLKETDSDILIATDPDCDRAGLTVKDGDNIRILNGNETGMLLLDYICNMRTQKGL
                     MPKDPIVIKTIVTTELAKNICKDYGIKIIEVLTGFKYIGEQINMFEEKGEKDSFIFGF
                     EESCGYLSGTYVRDKDAVNASVLICDMADYYKDKGISVLKRLGQLYKKYGYLLTYLGN
                     YTYNGIDGQKKMEKIMNSLRSGKIYFKDYLKEDGNLKADVLKFDISDMGIAIVRPSGT
                     EPKIKIYYSTQGYDMIEAENNMKKLVKYMERLIN"
     gene            4711..5874
                     /locus_tag="PLQ68_09010"
     CDS             4711..5874
                     /locus_tag="PLQ68_09010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HXK72832.1"
                     /translation="MQIKSESLKAHEQWQGKIEVISRVSLENKEQMSIAYTPGVAEPC
                     LEIYKDSENSYIYTRRHNMVAVITDGTAVLGLGDIGPLASMPVMEGKCALFKEFADID
                     AFPICIDSKDPDEIIRTIFLISKSFGGINLEDISAPRCFYIENELKKICDIPVFHDDQ
                     HGTAVVVLAALTNALKLKKQLLSDCKIVINGAGAAGSAICYLLLKAGAKNIIVCDVEG
                     ILYEGMDNLTSHKKDIAVKTNCKKIKGKLSDAVKASDVFIGVSRPNLLSIEDIKQMNE
                     KPIIFAMANPTPEIHPDDAKKAGAFVVGTGRSDFNNQINNVMAFPGIFKGALQCRAKQ
                     ITDDMLISAANAIASLVDDKDLSCEYILPKSFDKRLVLAVSQALVNKYNEQAK"
     gene            5929..6765
                     /locus_tag="PLQ68_09015"
     CDS             5929..6765
                     /locus_tag="PLQ68_09015"
                     /inference="COORDINATES: protein motif:HMM:NF039482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HXK72833.1"
                     /translation="MKFSLFANVNKDTNGAFIKKVTSFIESINCEVVYSQIITKGGSI
                     DLEKIKNTDIIITLGGDGTLLDVAQKTQQLSLPLVGINLGSIGFLTQIEKSNYEEFIT
                     LITQGHYTISRRMMIHAQLIRHDTVFDEYDALNDIVVSRKITGDTVSVSVNINGSYID
                     TYDGDGVIVSTPTGSTGYQMSAGGPVADPESEILLMTPICPHMMHRQSYVLRNDSILD
                     LNINETENYECCFSYDGITSDILGNDSIRITKSDSFVPLLKIQKNSFYENLRNKIYYR
                     GN"
     gene            6768..7217
                     /locus_tag="PLQ68_09020"
     CDS             6768..7217
                     /locus_tag="PLQ68_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909823.1"
                     /note="regulates arginine biosynthesis when complexed with
                     arginine by binding at site that overlap the promotors of
                     the arginine biosynthesis genes; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HXK72834.1"
                     /translation="MKNKRQVKILEFIEQYPIQTQEEMVSLLVKEGFEATQATVCRDI
                     KELKLFKAVNKDGLSVYTTSADISDTSHEKLVRVLKASFLSINIAQNIAVIKTLPATA
                     PAVASSIDNLKYKNLLGTIAGDDTIFACFTSNEDSQSFYEYILDELR"
     gene            7223..8914
                     /gene="recN"
                     /locus_tag="PLQ68_09025"
     CDS             7223..8914
                     /gene="recN"
                     /locus_tag="PLQ68_09025"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HXK72835.1"
                     /translation="MLLQLSIKNFAIFKDTIIDFNTGFSVITGESGAGKSVFITALSL
                     INGERALKEYIRKGCEYSSVEAVYKVNKDTFDNLTSKYEIDIEDNLLVITRQIYSESN
                     SICRVNGKIRNLSFLKEIASYLCDIHGQYENQTLLEPSSHQLIIDKMFDDKITPMNSE
                     YLEYLKEYDSVVNFILKNSGSEQQRSLRKDILSFQINEITESKIEQLDIDDLEKQRRI
                     LENSEKYINALKESIQSIEEGDSIEKIYLSSHILEKIRDMDEIVQISQSMMNSYYILK
                     DNFSQLKDITDEFYFDEEQYNDIIMKLELFKKLSYKYGDTIDKIRQFRENAEKQLYEI
                     ENFDNIYKLKIERIEKLENILYEKAEHLNSIRMSCALEFSKSVEENLHSLEMSDAIFN
                     VNFNKSIKKNTLGHYDFQKKGIYDIEFFISANKGIDPLPLAKIASGGEISRVMLAIKC
                     LITSKDDVDTYIFDEIDSGISGEAAVMTAIKLSSLSKEKQVICISHLPSIAAKADHHY
                     LIFKNSAKDMTFADIRELNHDDRIKQLAILFDGNTISQEGITHAKNLLEKMKKSN"
     gene            8954..9027
                     /locus_tag="PLQ68_09030"
     tRNA            8954..9027
                     /locus_tag="PLQ68_09030"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8986..8988,aa:Gly,seq:tcc)
BASE COUNT         3299 a         1342 c         1448 g         3011 t
ORIGIN      
        1 tgcttttagg ttttttatct taaaatgtcg cttgacattt tatagctggt cttaaatata
       61 cttcctaata attatatcat cataagaatt ttatacaata aagtataaac taattgtagt
      121 aaatgtatat gaaatgattt gtatatgagt tataataccc gttaggagaa atgatatgac
      181 tgtaaaaatt ttaactgatg aaaagaccta cgagattgaa tttaacaaaa taagtgctcc
      241 aaacatatat tcattaataa agagcatacc acaaattgat tttattgcac catgcggtgg
      301 cggacaacga tgcggtaaat gcaaaataaa gcttatcaat gacaatagtg atatatcaga
      361 ttctgaaagt aagtttttga cccctgctga aagaaaagat aatatcaggc ttgcatgctt
      421 tgtaaatgtt tcagatggtc agattattga tttgagaaat ataaaagcta ttcagtcgat
      481 aatgactgat aatcgactaa caaatagcaa aattgtcgtt aatccgctta taactgaagg
      541 atatggtgtt tctatagata ttggaacaac tactgttgct gcttctttgt atgatcttca
      601 aacagctaac agaatatctg ttacatcgga tataaacagg cagtcaagat tcggttctga
      661 tgtaataagc agaattcaat ttgcttcgac tcacgaaaat ttaatgatta ttcaagatac
      721 catattaaac gaaataaacg aattgataac aaaactatgc aaaagtaata atttatcaaa
      781 taatcaaatc aacttagtat atattgctgg caatactact atgcttcatc tttttatggg
      841 tttggatcca acaggaattg gagtagcacc ctttacgcct gttactcttg aaactcattt
      901 ttttagtcct gatgatgtta ctttgaaatc aattattaac attaataata aagggaaaat
      961 tatggtatgt gcttctattg caagttatgt aggtgcagac atagtagcag gaatattagc
     1021 atctggcata catacttctg acaaaccatg catattgcta gatgtcggaa ctaatggtga
     1081 aatagtatta ggtaataagg acaggatatt atgttgcgct actgctgcag ggccggcatt
     1141 tgaaggtgca aatatatctt gcggggttgc tggtatacaa ggtgcaataa atactgtaag
     1201 ctatgatgat gcaaacacct tcactacaat tggtgataag agtcctattg gcatatgcgg
     1261 ttcaggcctt atagatgcaa tttattcgat gttaaggaat ggaataattg aagaatcagg
     1321 ctggatggaa gatactgaag gcttcaagat aacagataat gttattttaa ctcaaaagga
     1381 tgtcagagaa gttcaaaatg cgaaagccgc aatagctgct ggtttaaaga ttttaattaa
     1441 gagatctggc tataaatact cagaaataga taaattgtac cttgcaggcg ggtttggcac
     1501 agtaatgaat gttgaaagtt ctgcaggtat tggacttata ccgccacagc tcaaggaaaa
     1561 agtcatacca gcaggtaata cttcattaac tggtataact atgctgttgc ttgacaaaac
     1621 aaatattgaa actattgaga atatcagaaa aattactgag tatatagaac taagccagga
     1681 tagcgaattt actgatgaat atgttgataa catgtttttt gagatatgaa cactaaaaaa
     1741 cagttaagaa tcgatatgac acaaaaaaga aatctgcttt ctaaagagca ggtaagtgct
     1801 ttgtcagaca acttatttaa ccgattctta aattctgatt taacaaagta caaaaattac
     1861 atgttatatc tacctataaa gaatgaaaca gatacatcta gccttataga ttatttactt
     1921 gaaaacaaaa aaaatgttta tgttccttat attagtgaca taaaaatagt tccagtcaat
     1981 attaatcaaa acacagaatt tgagcatgac tcatttgaga taagaatacc tatcgaaaaa
     2041 gttcagttag aatcaaattt acttgatgtt atttttgttc ctggactggc atttgataaa
     2101 gcaggttaca gagtaggata tggaaaagga tattatgata attttcttaa ggacttgtca
     2161 tgtattactt gtgcatggtg ttatgatttt cagattgtag ataagattgc agatataaaa
     2221 gaacatgata ttccagtaaa ttgttttata tgatttttat aaattacttt atattaaatc
     2281 tcgattcata ttttagtaat atgtaaattg aattaagtaa gatgtatatt gctgaaacaa
     2341 aaattaaccc caattgtata aagccagcat attgcgaaat atacttatca aataagttag
     2401 taacaatttg aactgaatat ataataaata aagaaccaca tactatatct aaaactctca
     2461 taaataagct tccgtatctt gaaagctctc gtttctcaaa aatgaaacat aacattaaaa
     2521 caataaaata tatagaatag gcaataagat ggctattgta cagaaatttt gtaatttcct
     2581 gaccatataa aaatccactt aaaaacaaca gtatattact tattagatga gcaggataga
     2641 gaatgtatga tatacgagtc ttaaatatcc taataaatcc aaagttaaat ataataagaa
     2701 gtaaccccat gaaaataagg ctgaaataaa aaggtttcag taaaatatca aaatctttag
     2761 tgaaaagatc agttgacaat ttaccaataa aaccattgtt aaataaataa taaattaata
     2821 cacctatact taaaaaacta tataagaaac caaatatctc aaatacaaat cttttatttc
     2881 ttttatgtga atttatatag gttagtttat gcatatctct taatttatca acctttccat
     2941 atattttacc aattttttca tattgttttc cgcttcaatc atatcatacc cttgtgtaga
     3001 ataatatatc ttaatctttg gttctgtgcc gctcggacgt acaatcgcta ttcccatatc
     3061 cgaaatatca aactttaata catctgcttt taaatttcca tcttccttta gataatcttt
     3121 aaaataaatc ttacctgatc tcaatgaatt cattattttt tccatttttt tctgaccatc
     3181 aataccatta tatgtgtaat tacccaaata ggttaaaaga taaccatact ttttatataa
     3241 ctgtccaagc ctcttaagaa cagatatacc tttatctttg taataatcag ccatatcaca
     3301 tatcagaact gaagcattaa cggcatcttt gtctcttaca taagttcccg ataaataacc
     3361 gcaactttcc tcaaaaccga aaataaagct atccttttct cctttttctt caaacatatt
     3421 tatctgttca ccgatatatt taaatcctgt caatacttca attattttta taccataatc
     3481 cttacagata tttttagcta gttctgtagt gacaattgtc tttattacta ttggatcttt
     3541 tggcattaac cctttttgag ttctcatatt acatatgtaa tccaaaagta acatacctgt
     3601 ttcattacca ttcaatattc taatattatc accatctttt acagtcagac cagctctgtc
     3661 acagtctggg tctgtagcta ttagaatatc agaatctgtt tcttttaaat ctctgatgcc
     3721 tagttctaat gcttctttta cttcaggatt aggatacgga catgtagtaa aattaccatc
     3781 aggataactt tgctctttaa caaaattgat ttttttaaat cctctttttt ctaaagctaa
     3841 actgacacat tccattccgc ttccattaag tggcgtatag actactttta actcacgttg
     3901 tcctgaataa aaagcttccg agcaaacagt gttaacataa gaaatagtaa tatcatcatc
     3961 tatgtaagtt ataagttttc tttcaagaca ttcttcgaaa ctcaactcat tattaatttc
     4021 atagaaaata tcagtctttt gcatattatg atatatctgc tcagctttgt caggatttat
     4081 ctgacatcct ttagaatcat atactttata accattataa tttgaaggat tgtgtgatgc
     4141 tgtaataacg attcctccat cacatttaag atgccttaca gcataagata gaagaggagt
     4201 gggagatacc ttgttaaaaa gataaacttt cattccacaa tttgcgagaa ctgttgctgc
     4261 ctctttagca aaggttaatg actttattct tgaatcataa gcaatcgcta cttttgagtt
     4321 attctttaat gtagccaaat aaccaaaggt ggcttttcga acagtatata tattcatcct
     4381 atttattcca gcaccgatta tccctcttaa tccacctgta ccgaattcaa gttgtgtata
     4441 aaatgcatct tcaataagat tgttatttga tttcattatt tttaattctt ttgtaagatc
     4501 attatccttt agtttatgag acatccatct tatgtattca ttattcatat ttgcttgtat
     4561 aatacggtca acgaccgttc attctccccc ttcttgtatt tacccataca ttatacaata
     4621 gacatcaaat atttacatat acacatactt tagtctattg tttttaacct ttagatatat
     4681 tatcatattt attgaaagga ttggttattt atgcaaatta aatccgagtc acttaaagca
     4741 catgaacaat ggcaaggcaa aatagaagta atatccagag tttcattaga gaataaagaa
     4801 caaatgtcaa ttgcatatac tccaggcgta gcagaaccct gtcttgaaat atataaagac
     4861 agtgaaaact cttatattta tacaagaaga cataatatgg ttgctgtgat aactgatgga
     4921 actgcagtac ttggactagg tgatattgga cctttagctt ctatgcctgt tatggaaggg
     4981 aagtgcgctc tatttaaaga atttgctgat attgatgctt ttcctatctg tatcgattca
     5041 aaagatcctg atgagataat aagaacaatt tttctgatat caaaaagttt tggtgggata
     5101 aatttagagg atatttcagc tccaagatgt ttttatatcg aaaatgaact aaaaaaaata
     5161 tgtgacatac ctgtatttca tgatgatcag catggtactg cagtagtagt attagctgct
     5221 ttgactaatg ctttgaaact aaaaaaacag ttactaagtg attgcaaaat agtgataaat
     5281 ggagctggag cagcaggaag cgctatttgt tatctattat tgaaagcagg tgctaaaaat
     5341 attatagttt gtgacgttga aggaattctt tatgaaggaa tggataattt aacatctcat
     5401 aaaaaagata ttgcagttaa gactaattgt aaaaaaatta aaggaaaatt atcagatgca
     5461 gtaaaagctt ctgatgtctt tattggagtc tcacgaccaa atctgctttc aatagaagat
     5521 attaaacaaa tgaatgaaaa accaattata ttcgcaatgg ccaatcctac tccggaaatt
     5581 catcctgatg atgcaaaaaa ggctggtgcc tttgttgtcg gtacaggaag aagtgatttt
     5641 aataatcaga ttaataatgt tatggctttt ccaggaattt ttaaaggagc tttacaatgc
     5701 agagcaaagc agataacaga tgatatgctt atatcagctg ctaatgctat cgcctcatta
     5761 gttgatgata aagatttatc ttgcgaatac atcttaccta agagttttga caaaaggctt
     5821 gttttagctg tctcacaggc tttagttaac aaatacaatg agcaagcaaa gtaatgtcta
     5881 ttgatattat ttggttttct catataatta aaggaggaag atcagtttat gaaattttcg
     5941 ctttttgcaa atgttaataa agatactaat ggtgctttca ttaaaaaagt aacttcattt
     6001 atagaatcta ttaactgtga agttgtgtat agtcaaatta ttacaaaagg cggtagcatt
     6061 gatcttgaaa aaataaagaa cactgatatc attattacat taggtgggga cggtacattg
     6121 cttgatgtag cacagaagac gcagcagctt tctttacctt tagttggtat taatttaggt
     6181 tctatcggtt ttttaactca aatagaaaaa tctaattatg aggaatttat aacacttatt
     6241 acacaaggtc attatacaat atccagaaga atgatgattc atgcgcaatt aataagacat
     6301 gacactgttt ttgatgaata tgatgcactt aatgatattg ttgtatctcg aaaaattaca
     6361 ggagatacag tctctgtatc agtaaatatt aatggttctt atatagacac atatgatgga
     6421 gatggtgtaa ttgtaagcac tcctacaggc tcgacaggtt atcagatgtc agctggaggg
     6481 cctgttgctg atcctgaatc agaaattctc cttatgactc ccatttgtcc tcacatgatg
     6541 cacagacaat cttatgtttt aagaaacgat agcatattag atttgaatat taatgaaact
     6601 gaaaattatg aatgctgctt cagttatgat ggtataacat cagatatttt agggaatgac
     6661 agcataagaa taacaaaaag tgattcattt gtcccattac tcaagattca aaaaaattct
     6721 ttttatgaaa atttgagaaa taaaatatat tataggggaa attagttatg aaaaataaaa
     6781 gacaggtgaa aatactagag tttatcgaac aatatcctat acagactcag gaagaaatgg
     6841 ttagtcttct tgttaaagaa ggttttgaag caacacaagc tactgtttgt cgtgatatta
     6901 aagaacttaa attattcaaa gctgtaaata aagatggatt atctgtttat acaacaagtg
     6961 cagatatttc agatacatca catgaaaagc tggtaagggt tctaaaagct tctttcttat
     7021 ccataaatat tgctcagaac attgctgtaa taaaaacatt acctgcaact gcacctgctg
     7081 ttgcatcatc tattgataat ttaaaatata aaaatctttt aggtactata gcaggtgatg
     7141 atacaatatt tgcctgcttt acttcaaatg aagattcgca gtctttttat gaatatatac
     7201 ttgatgaact gaggtaaata atttgttatt acaactttct attaagaatt ttgctatttt
     7261 taaagatacg attattgatt ttaatactgg attcagcgta ataactggtg aatcaggtgc
     7321 tggtaaaagt gttttcatta ccgctttgtc cctaattaat ggagaaagag cattaaaaga
     7381 atatattcga aaaggttgtg aatattcctc agttgaagca gtgtataaag tgaataaaga
     7441 cacatttgat aatcttacat ctaaatatga aatagatatt gaagacaatt tacttgtaat
     7501 tacaagacaa atttactcag aatcaaactc tatatgcaga gttaatggca aaatcaggaa
     7561 tttatcattc ctaaaagaaa ttgcatccta tctttgtgat atacatggac aatatgaaaa
     7621 tcagacctta ttagaacctt catcacatca gcttattatt gataaaatgt ttgatgataa
     7681 aattactccg atgaatagcg aataccttga atatctgaaa gaatatgatt cagtagttaa
     7741 tttcatatta aaaaatagcg gttctgagca gcaaagatca ttaagaaaag atatcctttc
     7801 ctttcaaatt aatgaaatta cagaatcaaa aattgaacaa ctagatattg atgatttaga
     7861 aaaacaacgt agaatacttg aaaattcaga aaaatatata aatgcactta aagaaagcat
     7921 acaatctata gaagaagggg acagtataga aaaaatttat ctttcatcac acattcttga
     7981 gaaaataaga gatatggatg aaattgttca aatatctcaa agtatgatga attcttatta
     8041 tatattaaaa gataattttt cgcagttaaa ggacataaca gatgagtttt attttgatga
     8101 ggaacagtac aatgacatta taatgaaatt ggagttattt aaaaagcttt catacaaata
     8161 tggtgatact attgataaaa ttcgtcaatt cagagaaaac gcagaaaaac agttgtatga
     8221 aattgaaaat tttgataata tctacaagct gaaaatagaa agaattgaaa aactagagaa
     8281 tattttatat gaaaaagctg aacatttaaa cagcataaga atgagttgcg cattggaatt
     8341 ttctaaatca gtagaagaga acttacattc tcttgaaatg tcagatgcaa ttttcaatgt
     8401 taatttcaac aaatcaataa agaaaaatac attaggtcat tatgattttc aaaaaaaggg
     8461 gatatatgat atcgaattct ttatttctgc aaataaaggt atagaccctc tgcctttagc
     8521 taaaattgct tcaggtggag aaatatctag agtaatgcta gcaataaaat gcctcataac
     8581 cagtaaggat gatgttgata cctatatatt tgatgaaata gattcaggta tcagtggcga
     8641 ggcagcagta atgaccgcaa taaaattgtc atctttatca aaagaaaaac aggttatctg
     8701 tatttcccat cttccttcaa tagcagcaaa agcagatcac cattatctga tatttaaaaa
     8761 tagcgcaaaa gatatgacat ttgctgatat aagagaatta aaccatgatg acagaataaa
     8821 acagctagct atactgtttg atggaaatac aattagtcaa gagggaataa ctcatgcaaa
     8881 aaaccttctt gaaaaaatga aaaaatctaa ttgacacata atatacttgt gttataatac
     8941 ataaggcaaa tgtgcgggtg tagttcagtg gtagaacact agccttccaa gctggttgtg
     9001 agggtccgat tcccttcacc cgctccattt tttttgtgcc agtagctcag ctggatagag
     9061 caacggactt ctaatccgta ggtcgggggt tcgaatccct
//
LOCUS       DAOSXM010000081         9072 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2111
            NODE_4886_length_9072_cov_10.397250, whole genome shotgun sequence.
ACCESSION   DAOSXM010000081 DAOSXM010000000
VERSION     DAOSXM010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864178
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 9072)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9072)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:24:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,261
            CDSs (total)                      :: 2,229
            Genes (coding)                    :: 2,218
            CDSs (with protein)               :: 2,218
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 3 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9072
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4886_length_9072_cov_10.397250"
                     /isolate="AMR_MDS_2111"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            87..626
                     /locus_tag="PLQ68_09035"
     CDS             87..626
                     /locus_tag="PLQ68_09035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis transcriptional
                     regulator"
                     /protein_id="HXK72836.1"
                     /translation="MTKATIKKQKRLDNLKNLLNDNPYMNDNELAKSLEVSVPCIRNY
                     RNELGIKEVRERIKDTVRALAPEEIVGTITELKLNEGGKAVFMTTSDMVFKKTNIVRG
                     QYIYSFAESVAISVIDADAALVGVANIKYKEPVYANEALTALAKVTQTRMNNYFVWVF
                     VYRDDKEIFRSKFIMVTIK"
     gene            635..1633
                     /gene="plsX"
                     /locus_tag="PLQ68_09040"
     CDS             635..1633
                     /gene="plsX"
                     /locus_tag="PLQ68_09040"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: protein motif:HMM:TIGR00182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HXK72837.1"
                     /translation="MNILVDAMGGDNAPEAIVVGCIEAIKASEGFKITLFGDESVIRQ
                     ILVKYKCNNNRIDIVHCSEKINSDDVPTFAVKNKKDSSMIKALNMQSQGFGDAVISAG
                     NSGALLAGSLLITGRIDGVIRPALGALVPSGNKFTLLIDAGLNSNLRAESYVQFAKFG
                     SEYMKIVSKCEDPVIGLLNIGTEDNKGSLDTKEANILLKNSKMNYYGNIEGNSIVEGI
                     VDVVVTDGFTGNVCLKLIEGTSSYIFGKMKAAFRIDIMHKLAAMIMKSDLKKFKDNLN
                     PDIMGGAPILGVNGLIIKSHGNSKIRTIKNVVLKTVSLLENDIVGNIKKAIMEPVD"
     gene            1736..1963
                     /gene="acpP"
                     /locus_tag="PLQ68_09045"
     CDS             1736..1963
                     /gene="acpP"
                     /locus_tag="PLQ68_09045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HXK72838.1"
                     /translation="MIFEKVKAIIVEQLGVDADSITMESSFIEDLGADSLDIVELIMS
                     MEDQFKITIPDTEAENISTVADAVNFIENNR"
     gene            2094..2810
                     /gene="rnc"
                     /locus_tag="PLQ68_09050"
     CDS             2094..2810
                     /gene="rnc"
                     /locus_tag="PLQ68_09050"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HXK72839.1"
                     /translation="MEKIIDDKAIAKVEKAIGYVFKDKTLLITALKHSSYDKNDNYEK
                     LEFLGDSVLQLVVSSYLFNTHKYLQEGEMTVTRAFAVCEETLSKICIRLGVDKFIHIG
                     MSGIASKVNKNRSVIADVFESIIGAIYMDSQFEYVKTFIIANINEFIVEYIKSGDNND
                     SKTKLQHFVQSQFQDEPKYVVKDEKGPPHDKTFTVEVYVNSKSYGKGKGKTKKEAQQA
                     AAKDALNKIEKKVWNNNDSQ"
     gene            2797..6345
                     /gene="smc"
                     /locus_tag="PLQ68_09055"
     CDS             2797..6345
                     /gene="smc"
                     /locus_tag="PLQ68_09055"
                     /inference="COORDINATES: protein motif:HMM:TIGR02168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HXK72840.1"
                     /translation="MILNKLELQGFKSFATRTVLEFNKGITAIVGPNGSGKSNIADAI
                     RWVLGSQSSKQLRGINMSDMIFSGTQFRKPVGFAEASLYFDNSDKGLAVDFDEVKITR
                     RLYRSGESEYSINNKNCRLKDIQMLFFDTGVGKEGYSIVGQNEVKDIIDSNSVERRGI
                     FEEAAGIMKYKQKKLEALRKLSNTEDNLIRINDITVEIENQLEPLRIQSEKAKEYLRV
                     YEILKTNEVGLYLNNLEMYKKRIIKFEQDIQIISDDVKVKEDELEKIKINNKDSNVII
                     GQYEEEENRLKEELNNFLRDLETSKHEIILCNERLSNATNEQIKNKKEIEEYELREKE
                     LSKDYLTKEDRRKYLEKQYLSFKEKLDALQQQADDIVQSLSGSGKVLENSKASLMNHL
                     EEASDLKLNINNVINEKQSLKNQIEMLESNISKTILENDSVLLAKQESQKSHLEIKKT
                     ISDITHARDKAKKDFSLYQKSMEQLRATEHSTKTNIEILQSNYKLIYDMEKHMQGYRQ
                     DVKAVLEKKDLDGIIDVVAKLFNTDESYNTAIEAALGGNIQNVVVEDEQSAKNAISFL
                     KENKLGRVTFLPVSSVRPNEFDKNQENEIMSLNGVIDIAYKLLDYDRRYDNIAKNLLG
                     KIVVVDTYDNAVLIAKKTQSRYKLVTLTGEFFNTGGSITGGTQHFSKVGLLGRQKQLE
                     RLDKEITDHKEKLSSTNSQIFTLIKQIDKISSEIDDLEKTISEKTMIAARLESSISSL
                     DDTYSNNLDALNNIKMKKSDLSDRIKGFDEKISILNQKLEALVQQIDLDKLTISDYEA
                     AQKEDTVKRDNIYLDISDLKVSVNSIEESLKSNDESLIRIGFEKQALVVKRNDIIKRN
                     AQLDVIKNDLKAQIEQKSKTIANPQKTQDGLNQKIKEVADKRKRLIAENEDYIQSITD
                     ANKDIMMLQNKISSLEVVKAKTDAEMSSMKERMWDEYNMTHNEALQKHSEIENITEAR
                     KQISYAKGVIKQLGPVNLESIEDYARTNERYRFFLTQKEDIEKSKEDLYKTINELNTV
                     MKKLFKEKFAIINENFNEIFIKLFDGGQARLELTDESDVLECGIDVVAQPPGKKLQNM
                     MLLSGGEKAFTAIALIFAMLRLNPSPFCIFDEIDTSLDDSNVLKYCNYLNDHKEKTQF
                     VLITHRKGTMENSDSIYGVTMQEHGITKVVSMQLTE"
     gene            6347..7276
                     /gene="ftsY"
                     /locus_tag="PLQ68_09060"
     CDS             6347..7276
                     /gene="ftsY"
                     /locus_tag="PLQ68_09060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013411935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HXK72841.1"
                     /translation="MNFFQRLKEGLSKTKDKLTKQIDKLLASFRKIDEELFEQLEEIL
                     IESDIAIDTVMEIIENLKNSVKINNITDPMDIRYLLKDNLYKILSKKDDNILFALNPK
                     NSNQLNIILVVGVNGTGKTTSIGKLSLMLSQTGKNVMLAAADTFRAAASEQLNEWAKR
                     TNVDIISHKSGADPGAVVYDAIHAAKKRNKDILICDTAGRLHTKSNLMEELKKIRRVI
                     TKEAEDAIVETVLVIDASSGQNSMVQARVFNECVHIDGIILTKLDGTAKGGIVVSICN
                     ELDIPVKYIGIGEKADDLKPFDAKEFVEALFDI"
     misc_binding    7304..7509
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            7569..>9072
                     /gene="ileS"
                     /locus_tag="PLQ68_09065"
     CDS             7569..>9072
                     /gene="ileS"
                     /locus_tag="PLQ68_09065"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010251292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HXK72842.1"
                     /translation="MYKKVTTEMDFTKREKETLEFWKKHNIFEKSIENRDGCPHFTFY
                     DGPPTANGKPHIGHILTRVIKDIIPRYKTMKGYKVLRKAGWDTHGLPVELEVEKLLGM
                     DGKEDIERYGVEPFIKQCKESVWKYKGEWEKMSDRVGYWADMENPYITYDNNYIESEW
                     WALKKIWDMGLLYKGHKVVPYCPRCGTSLSSHEVAQGYKDVTDKTIYVKFKVVNEPNT
                     YFMAWTTTPWTLPSNVALTVGKDFDYAKVEYNNELIIMAKQLVESVFEGLEYKIVGTV
                     KGKDLEYKKYIPLFDFADEETKEKGFYVCLGDFVTLTDGTGIVHTAPAFGEDDSAIGR
                     KYDLPFVQMVDESGHMKEDAEFIKGMFVKDADKEIAKNLYERGLLFKTANIEHSYPFC
                     WRCDTPLIYYARSTWFIKMTAIKESLLVNNDKINWIPETIKDGRFGNFLKSVVDWGIS
                     RERYWGTPLPIWECECGHREAIGSIEELKEKSDDCPENIDLHKPYIDNVHL"
BASE COUNT         3592 a         1138 c         1749 g         2593 t
ORIGIN      
        1 ataatttgtg tttctcttta caaatttact atcttagtct atactaaaat aaaatttgtt
       61 agcaggtgat aaatttaagg gagacaatga caaaagcgac aattaaaaaa caaaaaagat
      121 tagacaattt aaaaaatttg ctaaatgata atccatatat gaatgataat gagcttgcaa
      181 aaagccttga ggtcagtgtg ccttgtatta gaaattaccg aaacgaatta ggaataaaag
      241 aagtaagaga aagaataaag gatacagtac gtgcacttgc acctgaagaa atagtcggta
      301 ctattactga attaaaactt aatgaaggcg gtaaagccgt ttttatgact acatcagata
      361 tggtattcaa aaaaacgaat attgtaagag gacagtatat atattcattt gcagaatccg
      421 ttgcaatttc agttattgat gctgatgctg cgctcgttgg agtagctaat ataaaataca
      481 aagaaccggt atatgcaaat gaagcactga ctgccttagc aaaagtaacg caaacacgta
      541 tgaataatta tttcgtctgg gtattcgtgt acagagatga taaagaaatt ttcagaagca
      601 aatttattat ggtaacaatt aaatgagagg ttttatgaat atacttgtgg atgctatggg
      661 tggtgacaat gcaccggaag caatagtagt aggatgtata gaagcaataa aggccagtga
      721 agggtttaaa attactttat ttggtgatga atcggtaata aggcagattc ttgtaaaata
      781 taaatgtaat aataacagaa ttgatatagt tcattgctct gaaaaaataa attcagacga
      841 tgtgccaaca tttgcagtta aaaacaagaa agactcttct atgataaaag cattgaatat
      901 gcaaagccag ggatttggag atgctgtaat ctcagcagga aattcaggag cattacttgc
      961 tggttctttg cttattaccg gacgaattga cggagtaata agacctgcat taggagcatt
     1021 agttccatca ggtaataaat tcactcttct tattgatgca ggactcaact caaatcttcg
     1081 agctgaaagc tatgtgcagt ttgctaaatt cggttcagag tacatgaaga ttgtatctaa
     1141 atgtgaagat cctgttattg gccttttaaa cattggaaca gaagataaca aaggaagtct
     1201 tgatacaaaa gaagcaaata ttcttttgaa aaattctaaa atgaactact atgggaatat
     1261 agaaggaaac agcattgtag aaggtattgt tgatgttgtt gtaactgatg gatttacagg
     1321 aaatgtgtgc ttaaaattga tcgaaggtac atcctcatat attttcggta aaatgaaagc
     1381 cgcttttaga atagatatta tgcacaaatt agcagctatg attatgaaaa gcgatttaaa
     1441 gaaatttaag gataatctta atcctgatat tatgggaggt gctccaattc ttggagtaaa
     1501 tggactaatt attaagtctc acggaaattc aaaaataaga acaattaaga atgttgtatt
     1561 aaagacagtt tctctattgg agaatgatat agttggcaat ataaaaaagg caataatgga
     1621 accagttgat tagtatacta acgcaaaaag cgtttttata aaaataagtt atgttgacga
     1681 tggaataata atagcatatt ataaatataa tccatcgaag gaacgggagg ataaaatgat
     1741 atttgagaaa gtaaaagcaa tcatcgtaga acaattaggg gtagatgctg acagcataac
     1801 aatggaatct tcatttattg aggatctggg agccgattca ttagatatag tagaattaat
     1861 tatgtcaatg gaagatcagt ttaaaataac tatccccgac acagaagcag agaatatttc
     1921 tactgttgct gatgctgtta atttcattga aaataacaga taataaaact aggctattta
     1981 ttaatagcct tattttctta aaatttaaaa atcttaagaa aataagtatt agttgattta
     2041 gtgatgatca gttaaatggt tatcatgttt tacttagtta gatattaaaa aaaatggaaa
     2101 agattattga cgataaagca atagcgaaag tcgaaaaagc tataggttat gtttttaaag
     2161 ataagacatt actcataact gcacttaaac atagctcata tgataaaaat gataattacg
     2221 aaaaattaga gtttcttggt gattctgtac ttcaacttgt tgtaagctca tatttgttca
     2281 atacacataa atatttacag gaaggtgaaa tgacggttac aagagcattc gctgtttgtg
     2341 aagaaacttt atcaaaaatc tgtataaggt taggcgttga taaattcata catataggta
     2401 tgtcaggtat tgcttcaaag gtaaacaaaa accgatcagt tattgcagat gtatttgaat
     2461 ctattattgg agcaatctac atggattcgc aatttgaata tgtcaaaaca tttattattg
     2521 ccaatattaa tgaatttatt gtagaatata ttaaatcagg agataataat gacagcaaga
     2581 ctaagttgca gcattttgtc caatcacaat ttcaagatga accgaaatat gttgtgaaag
     2641 acgagaaagg ccctccgcat gacaagactt ttaccgttga agtatatgtt aacagcaaat
     2701 cgtatggaaa aggcaaaggc aagacaaaaa aggaagctca gcaagctgca gcaaaagatg
     2761 ccctgaataa aatagaaaaa aaggtttgga ataataatga ttctcaataa acttgaacta
     2821 caaggattta aatcatttgc aacacgtact gttcttgaat tcaataaagg aataacagct
     2881 attgtgggac ctaatggtag cggtaaaagt aacattgctg atgcaataag atgggttctt
     2941 ggttctcaaa gttctaaaca gcttagagga atcaatatga gcgacatgat attttcaggt
     3001 actcagtttc gaaaacctgt tggttttgct gaagcatcac tttattttga taattccgat
     3061 aaaggtttag cagttgactt tgatgaagta aagataacaa gaagactata tagatcaggt
     3121 gagtcagagt attcaattaa caacaagaat tgccgattga aagatataca gatgcttttc
     3181 tttgatacag gtgtcggcaa ggaagggtac tcaatagtag gacagaatga agttaaagat
     3241 ataattgaca gcaatagtgt ggaaagaaga ggaatctttg aggaagctgc tggtataatg
     3301 aagtacaaac agaagaaact tgaagcttta cgaaaattat cgaacaccga agataatctt
     3361 attagaataa atgatataac agtagaaatt gaaaaccagc tggaaccatt aagaatacaa
     3421 tcagaaaaag caaaagagta cttaagagtt tacgaaattt tgaaaactaa cgaagtcgga
     3481 ctatatctga acaatcttga gatgtataag aaacgaataa ttaaatttga acaggatatt
     3541 caaatcatat ctgatgatgt gaaagttaag gaagatgaat tagaaaaaat aaagataaat
     3601 aacaaagaca gtaatgtgat tataggccaa tacgaagaag aggaaaatag gcttaaagaa
     3661 gagttaaata actttttacg agatttagaa acttcaaaac atgaaataat tttatgtaat
     3721 gaaagattat caaatgctac taatgagcaa attaaaaaca aaaaagaaat agaagaatat
     3781 gagctaaggg aaaaagaact atctaaagat tatcttacta aagaagatag aaggaagtac
     3841 cttgaaaaac aatatctaag tttcaaagaa aaacttgatg cgctacagca gcaagcagat
     3901 gatatagttc aatcattaag tggaagcgga aaagttcttg agaattcaaa agcatcactt
     3961 atgaatcacc tagaagaagc ttcagattta aaactaaata taaataatgt aataaatgag
     4021 aaacaatctc ttaaaaatca gattgagatg cttgaatcaa atatatcaaa aacaatatta
     4081 gaaaatgact cagtgctttt ggcaaaacaa gagagtcaga aaagtcatct agaaattaag
     4141 aaaacaatat ctgatataac acatgcaaga gataaagcaa aaaaggattt ttctttatac
     4201 cagaaaagta tggagcaatt gcgtgcaact gaacacagta caaaaacaaa tattgaaata
     4261 cttcagagta attacaaact gatatatgac atggaaaaac atatgcaggg atatagacag
     4321 gatgttaagg ctgttttaga aaaaaaagat cttgatggta ttattgatgt cgttgctaaa
     4381 cttttcaata cagatgaatc atataataca gcaattgaag cagctttagg aggaaatatc
     4441 caaaatgtag tagttgaaga tgagcaatct gcaaaaaatg ctatatcatt tcttaaagaa
     4501 aataaattag ggagagtaac ctttcttcct gtaagttcag taagacctaa tgaatttgat
     4561 aaaaatcagg aaaacgagat tatgtcttta aatggtgtta ttgacatagc atataaatta
     4621 cttgattatg atcgaagata tgataatatt gcaaaaaatc ttttaggtaa aattgttgta
     4681 gttgatactt atgataatgc agtattaata gctaagaaaa cacagagcag atataaattg
     4741 gttactctta caggagaatt ttttaatact ggaggatcaa taacaggagg cacccagcat
     4801 tttagcaaag ttggtttact tggaagacaa aaacagcttg aaaggcttga taaggagatt
     4861 acagatcata aagaaaaact ttcatcgaca aattcacaga tttttactct tataaaacag
     4921 atagataaga tatcatcaga aattgatgat ttagaaaaaa caatatctga aaaaacaatg
     4981 attgctgcaa ggttagaatc atcaatttcc tctttagatg atacatatag taataattta
     5041 gatgccttaa ataatataaa gatgaaaaag agtgatttat cagacagaat aaaggggttt
     5101 gatgaaaaaa tatctatact caaccagaaa ctggaagctc ttgttcagca aattgattta
     5161 gataaactga caatcagtga ttacgaggcc gctcaaaaag aagatactgt aaaaagagac
     5221 aatatatatc ttgatatatc ggacttaaaa gtaagtgtaa actccattga agagagttta
     5281 aaaagcaatg atgagtcatt aattaggatt ggttttgaaa aacaggcttt agttgtaaaa
     5341 agaaatgata ttataaagag aaatgctcaa ctagatgtta tcaagaatga tttaaaagca
     5401 cagatagagc aaaaatcaaa aactatagct aatccacaaa aaactcaaga tggtcttaat
     5461 cagaaaatta aagaagtagc agacaagaga aagagactca ttgcagaaaa tgaagattat
     5521 attcaaagta ttacagatgc taataaagat atcatgatgc ttcaaaataa gatttcaagt
     5581 cttgaggtag taaaagcaaa gactgatgca gaaatgtcat caatgaaaga acggatgtgg
     5641 gatgaataca atatgactca taatgaggca ttgcaaaaac actctgaaat tgagaatatc
     5701 actgaagcaa gaaaacagat ttcatatgca aaaggagtta taaagcagct tggtcctgtt
     5761 aatcttgaat ctattgaaga ttatgctaga acaaatgaac gatacagatt ctttttaact
     5821 cagaaagaag acatagaaaa atcaaaagaa gacttatata aaactatcaa cgaacttaat
     5881 actgtcatga aaaaattatt caaggaaaag tttgcgataa ttaatgaaaa tttcaatgaa
     5941 atttttatta agctgtttga tggaggacaa gcaagactgg aactgactga tgaatcagat
     6001 gtgcttgaat gtggtattga tgttgtagct caacctcctg gaaaaaagct tcagaatatg
     6061 atgcttcttt ccggtggtga aaaagcattt acagcaattg cattaatatt tgcaatgtta
     6121 aggctcaatc cctcaccatt ttgtatattt gacgagatag acacatcact agatgatagt
     6181 aatgtactta aatactgtaa ttatttaaat gaccacaaag aaaagactca atttgtactt
     6241 ataactcaca gaaagggaac catggagaac tctgactcta tttacggagt aactatgcaa
     6301 gagcatggta taacaaaagt agtatccatg cagctgacag aataaaatga attttttcca
     6361 aagattaaaa gaaggattat caaaaaccaa agataagctg acaaaacaaa tagataaatt
     6421 actagcatca tttagaaaga ttgatgagga gcttttcgag caattggaag aaattcttat
     6481 tgaaagtgat atagcaattg atacggttat ggaaataatc gagaatctca aaaattcagt
     6541 taaaataaat aatattacag accctatgga tattagatat ctgttaaaag ataatttgta
     6601 taaaatttta tcaaagaagg atgataatat tctttttgca ctaaatccaa aaaattcaaa
     6661 tcaacttaat atcatattag tagttggagt taatggcaca ggaaagacta cttccatagg
     6721 aaaattaagt ctaatgttaa gtcagactgg taagaatgta atgcttgctg ctgctgatac
     6781 cttcagagca gcagcttctg agcagttaaa tgaatgggca aaaagaacca atgttgatat
     6841 tatatcgcat aaaagcggag ctgatcctgg agctgttgtt tacgatgcta ttcacgctgc
     6901 taaaaaaagg aataaagata tacttatatg tgatacagca ggaagactgc atacaaaaag
     6961 caatttgatg gaagaattaa aaaaaatccg tagagtaatt acaaaagaag cagaagatgc
     7021 aatcgttgaa actgttcttg taattgatgc ttcaagtggt cagaattcta tggttcaagc
     7081 tagagtattt aatgaatgtg ttcatataga tggtattatc cttacaaaac ttgatggtac
     7141 tgcaaaggga ggaattgttg tatcaatatg caacgaacta gatatacctg ttaaatacat
     7201 aggaataggt gagaaagcag atgatttgaa accttttgat gcaaaagaat tcgtagaagc
     7261 tttatttgac atttaataag aaatctgtgt ataataacaa aaagtaaagg cagggaaccg
     7321 aatagtaact atgctaaccc tttcagagag tatgataatg gtgtgaacat acaggtaggt
     7381 atagcgaata tcatcgggga gctggattcc agaacttaag taaggaatcc cgggttaaga
     7441 ccgttatccg aatcgagtgg cttttttaag caactagggt ggtaccgcga atgctctttc
     7501 gtcccttgag gatgagaggg tttttattat atatagccta aagaaaataa ggctggaaag
     7561 aggaaaaaat gtataaaaaa gtaacaacgg agatggattt tacaaaacgt gagaaggaaa
     7621 ctctagaatt ttggaagaaa cataacatct ttgaaaaaag tattgaaaac agagatggat
     7681 gtcctcattt tactttttat gatggaccac ctacagctaa tggaaaacct cacatcggcc
     7741 atatccttac tagagtcata aaagatataa ttcctagata taagactatg aagggatata
     7801 aagttttaag aaaagcagga tgggatactc acgggcttcc tgttgagctt gaagttgaaa
     7861 aacttttagg aatggatgga aaagaggaca tagaacgtta tggagtagaa cctttcataa
     7921 agcaatgtaa agaaagtgtt tggaaatata agggcgaatg ggagaaaatg agcgatcgtg
     7981 tcggttattg ggctgacatg gagaatccat atataactta tgataataac tatattgagt
     8041 cagaatggtg ggctttaaaa aagatctggg atatggggtt attatataaa ggacataaag
     8101 ttgtaccata ttgcccaaga tgtggaacaa gtttatcaag ccatgaagtg gcacaaggtt
     8161 ataaagatgt aacagataaa actatatatg tgaaatttaa agtagtaaac gagccaaata
     8221 cttattttat ggcttggact acaactcctt ggacattacc ttcaaatgtt gcacttaccg
     8281 taggaaaaga ttttgactat gcaaaagtcg agtacaataa tgaattaatt ataatggcaa
     8341 agcaacttgt agaatctgta tttgaaggtc tggaatataa aattgttggg actgttaaag
     8401 gtaaggattt agaatacaaa aaatatattc ctttatttga ttttgccgat gaagaaacaa
     8461 aagaaaaagg tttttatgtc tgtctaggag attttgtaac attaactgat ggtaccggta
     8521 ttgttcatac agctcctgca ttcggagaag atgattctgc aattggaaga aagtacgacc
     8581 taccttttgt tcagatggtt gatgaatcag gacacatgaa ggaagatgct gaatttataa
     8641 aaggtatgtt tgtaaaagat gcagataaag aaattgcaaa gaatctttat gaaagaggcc
     8701 ttttattcaa aactgctaat attgaacatt cttatccatt ctgctggaga tgtgatacac
     8761 ctttaatata ttatgcaagg tcaacatggt ttataaaaat gactgcaata aaggaaagtc
     8821 ttttagttaa taatgataag attaactgga tacctgagac aattaaggat ggacgttttg
     8881 gtaactttct aaaatctgtt gttgattggg gtatatccag agaaagatat tggggaactc
     8941 ctttaccaat ttgggaatgc gaatgtggac atagagaagc tattggcagt attgaagaat
     9001 taaaagaaaa atccgatgac tgtcctgaga atatagatct acataagcca tatattgata
     9061 atgttcattt aa
//
LOCUS       DAOSXM010000082         8920 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2111
            NODE_5004_length_8920_cov_9.438579, whole genome shotgun sequence.
ACCESSION   DAOSXM010000082 DAOSXM010000000
VERSION     DAOSXM010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864178
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 8920)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8920)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:24:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,261
            CDSs (total)                      :: 2,229
            Genes (coding)                    :: 2,218
            CDSs (with protein)               :: 2,218
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 3 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8920
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5004_length_8920_cov_9.438579"
                     /isolate="AMR_MDS_2111"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            70..675
                     /gene="lexA"
                     /locus_tag="PLQ68_09070"
     CDS             70..675
                     /gene="lexA"
                     /locus_tag="PLQ68_09070"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HXK72843.1"
                     /translation="MKDYLSIREEQTYEYIRDFIREKGFAPSVRDICKDLNIPSTSTA
                     HSYINKLRRLGYIEKNDSISRSLTLSKQESVSVVEIPVIGDVAAGTPILAVENIVEYF
                     PVPEAYLKSGITFMLKVKGDSMINAGIHDNDLILVKKQEVAYDNDIIVALIDNETTVK
                     TFFRERDRIRLQPANDEYEPIYVYKDLQILGKVFGVFRIFN"
     gene            complement(676..1590)
                     /gene="miaA"
                     /locus_tag="PLQ68_09075"
     CDS             complement(676..1590)
                     /gene="miaA"
                     /locus_tag="PLQ68_09075"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002597820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HXK72844.1"
                     /translation="MNKALIVTGPTASGKSDIAIKIAKKLNGEIVSADSMQIYKKMDI
                     GTAKPDLFQLNEVKHHMIDIIDIEDNYSVARYKDEAFKCIKDILSRNKVPIITGGTGL
                     YIDSLLYGISFDNEYPENKRRFELYLKEHGKEELFKLLLERDNQAALTTHPNNTKRVI
                     RYLDILSRFKGTLSEYKQQAIKNDSSVDFNVFVLNPPRDKLYERIESRVDIMIEKGLI
                     EEVSLILKSQIDSNCTSMQAIGYKEVVKYLDGYCTYDEMIRILKMNTRNYAKRQITWF
                     KRYKEAQFIDTDKFENSNEITRFIISCL"
     gene            complement(1590..3437)
                     /gene="mutL"
                     /locus_tag="PLQ68_09080"
     CDS             complement(1590..3437)
                     /gene="mutL"
                     /locus_tag="PLQ68_09080"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HXK72845.1"
                     /translation="MSGKIIILDEFTANQIAAGEVVERPASVLKELIENSIDAKADRI
                     DIEIKNGGIGYIKVSDNGNGIDKDDIPMAFERHGTSKIRQISDIYNISTMGFRGEALP
                     SIASVSNVSITTKTKEQDFAVKAVYEALEQKSISKSARAQGTTIEVKNLFFNTPARYK
                     FLKGDKQEARYCTDIVLRLALANTQVAFSMISNGQEVIRTNGDNLLINCIYGIYGRDI
                     AKDIIEINAETDDFSVSGYIGRPFASRGNRQHQSLFINNRYVKYPELNTSIERAYETR
                     IMKGRFPFYVLKLSISSQNVDVNVHPSKIEVKISNISEVFGKLNYILKESLDKEQKVM
                     EPSVIRPSYTKQKEQEEAKTILDFNNQLYTFSKELQDIKVEESKVEGNTVKELIANYQ
                     NYKKEGSVKIEDVITCENTLKTTETNSSGQIQQSTMEPYRIAGQILNTFIIIDKFDYI
                     YIIDQHAAHEKINFEKLKNEYESKIITSQQLMIPVELVITSSEMQFVESNEDIIKSFG
                     FDFSQFGKNSIIIREIPSSLAECDIEDAFRAMIDDMQNDKKDKIYTALYSIACKASVK
                     ANKKLDEEQIKYIYEKLMTLKNPFTCPHGRPTIIKMTRYDFDKLFKRIV"
     gene            complement(3450..6014)
                     /gene="mutS"
                     /locus_tag="PLQ68_09085"
     CDS             complement(3450..6014)
                     /gene="mutS"
                     /locus_tag="PLQ68_09085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015925163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HXK72846.1"
                     /translation="MENLTPMMKQYLDTKQQYKDCILMFRLGDFYEMFFDDALTASKE
                     LEITLTGRNCGLDDRAPMCGVPFHSVDPYIAKLIKKGYKVAICEQLEDPATAKGLVKR
                     DVTRIITPGTVNENTLLEEKENNYIMSLFKRGEVYSLAVCDVSTGALYSCQIIYGNIL
                     NKLLNEVVKFKPKEIISNFDAKFVKEHISVYFTDVEPKIFDSKLPDIKIASKIKVNDF
                     DIWRNACAGLYQYLKDTRKADLSHITSVNVYEPEKFMNLDSIARQNLELTENLRDKSR
                     YGTLLWVLDKTSTSMGSRMLKKWIEQPLTDLSDIDLRLDAVSDIKDKYIVRQELMELF
                     KSIYDIERLVSKTVMANANGKDLLALKASFYKLPFIKQLLSKLQAALCIQLNEDINCF
                     ETLHKLIEDSINEDCPVILREGKLIKEGFDEQVDELRKAAHGGKDWLIKFEEKEKERT
                     GIKKLKVSYNKVYGYYIEITNSFVDQVPQDYIRKQTLVNSERYITQELKDMEESILGA
                     EEKLIRLEFELFCKIRDTIASSAESLLKTAYSLSVLDALCSFAEVADRYNYNRPTIYD
                     KCEIDIKSGRHPVVERMPNCSEFIPNDLYINNDKDRTLIITGPNMAGKSTYMRQAALI
                     TIMAQIGSFVPADSANIGVTDKIFTRIGATDDLAGGKSTFMMEMSELAYILENASDRS
                     LLILDEIGRGTSTFDGLSIAWAVIEYLNETRRCRTLFSTHYHELTVLEDKFEGVRNYY
                     ISAIKKDKDIVFLRKVKRGAANESYGIEVARLAGVPEQITVRAGEFLTELERSENKRS
                     RIKKKEAYMDGQVGILEFNNQGNGNKEIIDILKQCDIQKMTPLEALNKLYELKSKV"
     gene            complement(6014..6412)
                     /locus_tag="PLQ68_09090"
     CDS             complement(6014..6412)
                     /locus_tag="PLQ68_09090"
                     /inference="COORDINATES: protein motif:HMM:NF017902.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HXK72847.1"
                     /translation="MIFEKVDELCELIEETKQYNDYLEAEVELENDGHGIDLIKDFND
                     LQQEYINVYNEGKQDEVVSMQNILESRHTELLAYPTTGAYIRAKSALDDLLTEINTRI
                     LKSLGLTSNSEGCDSCSSDSCEDGCCGGCH"
     gene            complement(6409..>7473)
                     /gene="miaB"
                     /locus_tag="PLQ68_09095"
     CDS             complement(6409..>7473)
                     /gene="miaB"
                     /locus_tag="PLQ68_09095"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HXK72848.1"
                     /translation="CMMQQKHVVEKIKNSYKYIDIVFGTHNRHELPKMLLSRLSTGKK
                     VYEIWEDNKGIAENVPVERKSNIAAFVPITYGCDNFCSYCIVPYVRGRETSRDPEKIK
                     QDIIELSQKGIKEITLLGQNVNSYNYGMDFSKLLYYISDVPGIERIRFMTSHPKDLSD
                     NLIKAMTEIPSMCNHIHLPLQSGSTEVLRQMNRKYSKEDYLLLVSKIRQAIPEISIST
                     DIIVGFPGETEYDFNETLDVYKKTGFDFAYTFLYSKRTGTPAAKRIDQIDDDVKGKRF
                     KRLVDLVNEMTLSSNIKDENKILKVLVEGKSRTDDKIMTGRTESNKIVNFRCDSDFTG
                     KIVNVKIIKGHTWHLEGEKI"
     assembly_gap    7474..7573
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(7632..8021)
                     /locus_tag="PLQ68_09100"
     CDS             complement(7632..8021)
                     /locus_tag="PLQ68_09100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72849.1"
                     /translation="MDNTMNEYEIIEQMEDLITSAKQTVFSDKVSISKDEMLDLIYYL
                     KNAIKQRDQEEEAKNKELLRMIQETKSKEQVSVQPVGVIIEDAHKEANDIKKAALKYA
                     DELLASIEGKIEKTLHIIKENRDELNQ"
     gene            complement(8026..8517)
                     /gene="coaD"
                     /locus_tag="PLQ68_09105"
     CDS             complement(8026..8517)
                     /gene="coaD"
                     /locus_tag="PLQ68_09105"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020814584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HXK72850.1"
                     /translation="MKIWICPGSFDPVTKGHIDIISRACLMCDKLIVAVGENKQKKSV
                     FTLEERIDFIERSLNGMKNVEVESFHGLLIDYARSKNAIGIVKGLRAVSDFEYEMQMA
                     FLNKRMDENIETVFLMTNMNYSFLSSSAVRELALHGGPIEGLVPDSIIEDVRRKYHIN
                     SVR"
     gene            complement(8486..>8920)
                     /locus_tag="PLQ68_09110"
     CDS             complement(8486..>8920)
                     /locus_tag="PLQ68_09110"
                     /inference="COORDINATES: protein motif:HMM:NF015559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HXK72851.1"
                     /translation="IFSGTGSIGLEFLSRGAEKAVFIDKDISITKSNVSKCRFEDKTV
                     TIKSDWQKGLAKLKEEKYRFTHIFLDPPYGSGLYERVIENSDLYDIMDKNAVVIAELS
                     EEDTVLEKTSNLILIDKRKYGICVFAFYVKGNYEDLDLSGQF"
BASE COUNT         2583 a         1693 c         1168 g         3376 t
ORIGIN      
        1 gaaacatttg tttacacgaa cttatgtttt atgttataat ttgtatataa aatacatgga
       61 ggcttcataa tgaaagacta cttatccatc agagaggaac agacttacga atatataagg
      121 gatttcatac gtgagaaagg ctttgctcct tctgtaaggg acatatgcaa agatcttaat
      181 attccttcaa cctcaactgc tcactcatat attaataaac tcagaagatt aggatacatt
      241 gaaaaaaatg actctatttc acgttctctg accttgtcaa agcaggaatc agtatcagta
      301 gttgaaatcc cagtcatcgg tgatgtagct gcaggaactc ccattcttgc tgttgagaac
      361 attgtagaat actttcctgt tcctgaagct tatcttaaaa gtggtataac ctttatgctt
      421 aaagtcaagg gagattcaat gataaatgca ggtattcatg acaacgacct tatacttgtt
      481 aaaaaacagg aagttgcata tgataatgat attattgtag ctttaattga taatgaaaca
      541 acagtaaaaa ctttcttcag agaaagagac agaattcgtc ttcagccagc aaatgatgaa
      601 tatgaaccta tatatgtcta taaggactta cagatattag gaaaagtatt cggtgttttc
      661 agaattttca actaactata aacagctaat aatgaacctt gttatttcat tactgttttc
      721 aaatttatca gtatcgataa actgagcttc tttatacctt ttaaaccatg taatttgtcg
      781 ttttgcatag tttctagtat tcatcttaag aattcttatc atttcatcat aggtacagta
      841 accatcaaga tacttaacta cttccttata ccctattgcc tgcattgatg tacagttact
      901 atctatctga cttttcaata ttaaagaaac ctcttcaatt aaaccttttt ctatcattat
      961 gtcaactctt gattcaatgc gttcatataa tttatcacgt ggaggattaa gcacaaagac
     1021 attaaagtca acactggaat cattcttaat tgcttgttgc ttatattcac taagagttcc
     1081 tttaaaccta cttaatatat caagatatct gattactctt tttgtattat taggatgtgt
     1141 agtcaaggct gcttgattat ctctttctaa aagcaattta aataattctt ctttaccatg
     1201 ttcttttaaa tacagttcaa atcttctttt attttctgga tattcgttgt caaaactaat
     1261 tccatatagt aatgaatcta tatataggcc tgtcccacct gtaataattg gaactttgtt
     1321 tcttgataaa atatctttga tgcatttaaa ggcttcatct ttgtatcttg caacagaata
     1381 attgtcttca atatcaatta tatctatcat gtgatgtttt acttcattca attgaaataa
     1441 gtcaggtttt gcagtaccaa tatccatttt tttataaatc tgcatagaat cagcagaaac
     1501 tatttctccg tttagtttct ttgctatttt gattgcaata tcagattttc ctgaagctgt
     1561 cggtcctgta actattaatg ccttattcat tatacaattc tcttaaaaag cttatcaaaa
     1621 tcatacctgg tcatcttaat tattgtaggt ctaccatgtg ggcatgtaaa tggatttttt
     1681 agagtcatca gtttttcgta aatatactta atttgctctt catcaagctt tttatttgct
     1741 tttacagaag ccttacaggc aattgaatac agagcagtat aaatcttatc ttttttgtca
     1801 ttctgcatat catcaatcat tgctctaaaa gcatcttcaa tatcacattc agctaacgat
     1861 gatggaattt cccttatgat aattgagttt tttccaaact gactgaaatc aaatccaaat
     1921 gacttaataa tatcttcatt actctcaacg aattgcattt ctgaacttgt tattacaagc
     1981 tctacaggta tcataagttg ctgtgatgtt attattttac tttcatattc attctttaac
     2041 ttttcaaagt ttattttttc atgagctgca tgttgatcaa taatataaat ataatcaaat
     2101 ttatctatta ttataaatgt attaagtatc tgccctgcaa ttctgtatgg ttccattgta
     2161 ctttgctgta tttgaccaga ggaattagtt tctgttgttt ttagagtatt ttcacatgta
     2221 attacatctt ctatttttac agatccttct tttttataat tctgatagtt agcaatcaat
     2281 tcttttactg tatttccttc tactttactt tcttctactt taatatcttg aagttctttt
     2341 gaaaaagtat aaagttgatt attaaaatct aaaatagtct ttgcttcttc ctgctctttc
     2401 tgttttgtat atgaaggcct tataactgat ggttccataa ctttctgttc tttatcaaga
     2461 gattctttta aaatatagtt taatttaccg aatacttcag aaatattgga tattttcact
     2521 tcgatttttg atggatgtac attcacatca acattctgtg aagaaatact taattttaga
     2581 acataaaatg ggaatcttcc tttcatgatt ctagtctcat aggccctttc aatagaagtg
     2641 ttcaattcag ggtattttac atatctgtta tttataaaaa ggctctgatg ttgtctgttt
     2701 cctcttgacg caaatggtct gcctatatat cctgatacag aaaaatcatc ggtttctgcg
     2761 ttgatttcta tgatatcttt cgctatatct cttccatata tgccatatat gcagtttata
     2821 agcaggttat ccccgtttgt ccttattacc tcctgtccgt tggatatcat agaaaatgca
     2881 acctgtgtat ttgcaagagc cagccttaaa actatatctg tacaatatct tgcttcctgc
     2941 ttatcacctt ttaaaaactt atatcttgca ggtgtgttaa aaaacaaatt tttaacttca
     3001 attgttgttc cctgtgctct ggctgatttt gatatagatt tctgttcaag agcctcatac
     3061 acagctttta cagcgaaatc ctgctctttt gtctttgtag taatggaaac atttgaaact
     3121 gaagcaatgc ttggcagtgc ttctcctctg aatcccatag ttgatatatt gtatatatct
     3181 gaaatctgtc ttattttact tgttccatga cgttcaaatg ccatcggaat atcatcttta
     3241 tctattccat ttccgttatc agatactttg atataaccaa taccgccatt ttttatttct
     3301 atatctattc tgtcggcttt agcatcaata gagttttcaa taagctcctt aaggaccgac
     3361 gcaggacgtt ccactacctc tcctgcagca atctggttgg cagtaaactc atcaagtatt
     3421 ataatcttac cgctcatgaa ttattccttt catactttac tcttaagctc atataactta
     3481 tttaaagctt ctagaggtgt catcttctga atatcacatt gtttcaatat atctataatc
     3541 tctttatttc cgttcccctg attattgaat tctaagatac ctacctgccc atccatatat
     3601 gcttcctttt ttttaatgcg acttctttta ttctcgcttc tttccagttc tgttaagaac
     3661 tctccagctc taaccgttat ctgttctgga acgcctgcta atcttgcaac ttcaatacca
     3721 tagctttcat tagccgctcc ccgttttact tttctaagaa agacaatatc cttatctttc
     3781 ttaattgctg aaatatagta atttcttact ccttcaaatt tatcttctag aacagtcagt
     3841 tcgtgataat gagtggaaaa caaggttcgg cagcgtcttg tttcattaag gtattctata
     3901 actgcccaag caatggataa tccatcaaat gtactagttc ctctgccaat ttcatcaagt
     3961 attaataatg atctgtctga agcattttca agaatatatg caagctcgct catttccatc
     4021 ataaatgtac ttttaccacc tgccaaatca tcagttgctc ctattctggt aaatattttg
     4081 tcagtaacac caatattcgc agaatctgcg ggtacaaatg atccaatttg cgccataatg
     4141 gttataagag cagcttgtct catatatgtc gactttccag ccatgtttgg tcctgtaatt
     4201 atcaaggttc tgtccttgtc gttatttata taaagatcat ttggtatgaa ttcagaacaa
     4261 ttaggcattc tttctacaac tggatgcctt ccgctcttaa tatctatttc acatttatca
     4321 tatatggttg gacgattgta gttatacctg tctgcaactt ccgcaaaaga gcacagtgca
     4381 tccagaacgg aaaggctgta tgctgttttt aaaagagatt cagcgctaga tgctattgta
     4441 tcccttattt tgcaaaaaag ctcaaattca agccttataa gcttctcctc tgcaccaaga
     4501 atggactctt ccatatcttt cagctcctga gtaatatatc tttcactgtt tacaagagtc
     4561 tgttttctta tatagtcttg tggtacctgg tcaacaaaac tgttagttat ttcaatatag
     4621 taaccatata ctttgttata tgatactttg agttttttta taccggttcg ttctttttct
     4681 ttttcttcaa acttaataag ccagtcttta cctccatgag ctgcttttcg aagttcgtca
     4741 acctgctcat caaagccttc ttttataagc ttgccttccc taaggattac tggacaatct
     4801 tcatttatgc tgtcttcaat cagtttgtgt aatgtttcaa aacaattaat atcttcatta
     4861 agctgtatac ataaagctgc ctggagttta cttaacagct gttttataaa cggcaattta
     4921 taaaaagaag cttttaaagc taacaaatct ttcccattag cattagccat tacagttttt
     4981 gaaactaatc tttcaatgtc ataaatgctt ttaaaaagtt ccattaactc ctgtctgaca
     5041 atatatttat ctttgatatc actaactgca tccagtctta aatcgatatc agacaaatca
     5101 gttaagggtt gttcaatcca ctttttaagc attcttgaac ccattgaagt agatgtcttg
     5161 tccaaaaccc ataaaagtgt tccatatcgt gatttatctc ttaagttttc agtaagttca
     5221 agattttgtc ttgcaatact gtccagattc ataaattttt ctggttcgta tacattcaca
     5281 gaagtaatat gagacaaatc agcttttcga gtatctttta agtattggta caggccagca
     5341 caggcatttc gccaaatatc aaaatcgttg acttttattt tacttgcaat ctttatatca
     5401 ggtaatttac tatcaaatat ctttggctca acatcagtaa aatatactga aatatgctct
     5461 tttacaaact tcgcatcaaa attggaaata atctccttag gtttgaattt gactacctca
     5521 tttaatagct tatttagaat attaccatag attatctggc aagagtataa tgcacctgtg
     5581 gatacatcgc acacagctaa tgaatatact tcacctcttt taaataagga cattatataa
     5641 ttattctctt tttcctcaag caatgtgttt tcatttactg ttccgggtgt aattattcta
     5701 gttacatctc gtttaaccaa cccttttgca gtagctggat cttccaactg ttcacatata
     5761 gctaccttat atcccttctt aatcagtttc gcaatatatg gatcaacgct atgaaatggg
     5821 actccacaca tgggagccct atcatccaga ccgcaattcc tacctgttag cgttatttcc
     5881 aattcttttg atgctgttaa tgcgtcatca aaaaacatct cataaaagtc acccagtcga
     5941 aacataagta tgcaatcttt atactgctgt ttagtatcaa gatattgctt catcatgggt
     6001 gttagatttt ccattaatga cagcctccgc aacaaccgtc ttcacaagaa tctgaagaac
     6061 aactgtcaca accttcacta tttgatgtaa gtcctaaact cttaagtatt cttgtattta
     6121 tctcagtaag taaatcatca agcgcacttt ttgcacggat atatgcacct gtagtaggat
     6181 aagcaagaag ttcagtatgt ctgctctcaa gtatattctg cattgagaca acctcatctt
     6241 gcttaccttc attgtataca ttgatatatt cttgttgaag atcattaaaa tcttttataa
     6301 gatcaatacc atgaccatca ttctccaatt caacttctgc ttctaaataa tcattgtatt
     6361 gttttgtttc ctcaatcagt tcacataatt catctacttt ttcaaatatc atattttttc
     6421 tccttctaaa tgccatgtat ggccttttat tattttaaca ttaacaatct tacctgtaaa
     6481 atcagaatcg catctgaaat taactatttt attgctttca gttctaccgg tcataatttt
     6541 atcatcagta cggcttttac cttccaccag aacttttaat atcttatttt catcttttat
     6601 attactgctc aatgtcattt cattaacaag atcaactaat cttttaaatc ttttaccttt
     6661 aacatcatca tctatctggt ctattctttt tgctgcagga gttcctgttc tttttgaata
     6721 taggaatgta tatgcaaaat caaatcctgt tttcttataa acatccaaag tctcattgaa
     6781 atcatactca gtttctcctg gaaatccaac tattatatct gtacttattg agatttcagg
     6841 tatagcctgt ctaattttac tgactaataa aagataatct tctttagaat attttctgtt
     6901 catttgtctt aatacttcag tgcttccaga ttgcagtggc agatgtatat ggttacacat
     6961 tgatggtatt tcagtcattg ctttaataag gttatccgat aaatctttcg gatgactggt
     7021 cataaatctg attctttcta tacctggaac atcagatatg taataaagaa gttttgaaaa
     7081 atccattcca taattatatg aattgacatt ctggccaagc aaagttattt ccttaattcc
     7141 tttttgtgat aactctataa tatcctgttt tattttttcc ggatctcttg atgtttctct
     7201 tcctctgaca taaggaacta tacaatatga acagaaatta tcacatccat atgtaattgg
     7261 tacgaatgca gctatattgc tctttctttc tacaggaaca ttttccgcaa tacctttatt
     7321 atcttcccag atttcatata cttttttacc tgttgaaagc cttgatagta acatttttgg
     7381 taactcatgc ctattgtggg ttccaaacac tatatctatg tatttatagc tgtttttaat
     7441 tttttctaca acatgctttt gttgcatcat acannnnnnn nnnnnnnnnn nnnnnnnnnn
     7501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7561 nnnnnnnnnn nnnatagata aataaaatac ccgttacctt tatggcaacg ggtatgttga
     7621 tttaatatcc gttactgatt taattcatcc ctgttctctt taataatatg taatgttttc
     7681 tctatttttc cctctattga tgccaagagc tcatctgcat actttagtgc agctttttta
     7741 atatcattag cttctttatg agcatcttca ataatgacac ctactggttg aacagatact
     7801 tgttctttac tctttgtttc ctgaatcatt ctgagtaatt ccttgttctt ggcttcttct
     7861 tcctgatctc gctgctttat tgcattttta aggtagtata ttagatcaag catttcatcc
     7921 ttgcttatag ataccttatc agaaaaaact gtttgtttag cacttgtaat cagatcttcc
     7981 atctgttcga tgatttcata ttcattcatt gtattgtcca ttatattacc tcacactatt
     8041 tatatgatat tttcttctaa catcttctat tatactatct ggtacgagtc cttcaatagg
     8101 tcctccatgt aatgcaagtt ctcttactgc agatgaactt aagaatgagt aattcatatt
     8161 agtcataaga aatactgttt caatattctc atccattctc ttgtttaaaa atgccatttg
     8221 catttcatat tcaaagtcag ataccgcacg taatccttta acaataccta ttgcattttt
     8281 actacgagca taatcaatca gcaaaccatg aaagctttca acttcgacat tcttcattcc
     8341 atttaaagac cgttctataa aatcaattct ttcttctaat gtaaatacac tttttttttg
     8401 cttgttttca cctactgcta caataagctt atcgcacata agacatgctc ttgatattat
     8461 atctatatgt cctttcgtta ccggatcaaa actgcccgga caaatccaaa tcttcataat
     8521 tcccctttac ataaaatgca aatacgcaga taccgtactt tctcttatca atcagaataa
     8581 gattggaggt cttttccaaa actgtatcct cttctgacaa ttctgcgatt acaacagcat
     8641 ttttgtccat tatatcatac aaatcactgt tttcaataac tctttcatac aagccagagc
     8701 catatggagg atcaagaaat atatgtgtaa atctatactt ctcttctttt aacttagcta
     8761 atcctttttg ccagtctgac ttaattgtaa ctgttttatc ttcaaacctg catttagata
     8821 cattactttt tgttattgat atatctttat caatgaaaac tgccttttct gctcctcttg
     8881 atagaaattc taaaccaata ctgccagttc ccgagaaaat
//
LOCUS       DAOSXM010000083         8908 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2111
            NODE_5015_length_8908_cov_10.770247, whole genome shotgun sequence.
ACCESSION   DAOSXM010000083 DAOSXM010000000
VERSION     DAOSXM010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864178
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 8908)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8908)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:24:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,261
            CDSs (total)                      :: 2,229
            Genes (coding)                    :: 2,218
            CDSs (with protein)               :: 2,218
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 3 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8908
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5015_length_8908_cov_10.770247"
                     /isolate="AMR_MDS_2111"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1018..2655
                     /locus_tag="PLQ68_09115"
     CDS             1018..2655
                     /locus_tag="PLQ68_09115"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXK72852.1"
                     /translation="MLSNLFKKVIEENKSDAVVGLFFGIIIAPAAPILFQIGIQKFVD
                     YTFGDNNYPIIWILLALVILNIGSLYSGKFIYRKIENRLDLSYKTSISEIFTRKLKRD
                     NNLSRGEIIQTIEIIDDVVDFKKEKIMFLPLVISFIFIVLYFIFNLNGLYLLQYILYA
                     IPIIIVLIVMTNIIGKYQKRVLEEAGKANDISTEIIEGFDIITNFRATDHFKDTFSRN
                     NNYVRKFKNKINLTENIIDITTILINISLIIAMPLAASFLYKSGKITVGGIFIASSLI
                     QQITEIISKIVGFFASNAKNKKYMELLNNIENSVVSTEGCNTAEINNFDKLYIKKLSF
                     TYDDKNMILDNLSYEFEKNNIYIINGVSGIGKTTLIKLIVNELEIQSGEITLDNVNIS
                     PEIVKDLVSYSSQQSFLFHGNIEENIVYGINQDVNETQKKELVGNLEKVFNFDNIFKK
                     EKALENIEINSQSDNISLGQKKLICLARTIIKDAKLVILDEPTANLNIRQVDIFKNEL
                     VKLKEDRIIIVITHDKRLNEIASRVLSIVDGVIVNEQ"
     gene            2645..4261
                     /locus_tag="PLQ68_09120"
     CDS             2645..4261
                     /locus_tag="PLQ68_09120"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXK72853.1"
                     /translation="MSNKKVPYINSTKEQLIGIGLEIISQSANALAFIIFTLIAGNII
                     NGVIDSNSVLIESTLKKELPICLILLVIVYPIFKYSANIYKNRKIFVLKECMLDLLFE
                     MSIKIQSNQFLNNFNMDINDIEKVIERIYPGIINSLLTSIYAIVLLSVGYDFMFVIIL
                     VTTIIFNLLGGYFALRNFKLEGEINEKKRDGNSHILNIFSNNLIFNIFPNARLFLNKT
                     IDILTKIRKDKDIQAKNNTYYNILFYVSEVIRVVGVVYIGARYTDIGIGGITSLLLLI
                     AQSSVLFRDFSENLLGLKKFSIAKNKLSETFLMENSTTKETEVEVERNINDEKGIEFR
                     NVFFSYNSGSDLFKGLSFKANNNELTLIKGDIGRGKSTMLKLILKKLNVNDGNIYING
                     KSINILDDSDINKLITYVDQGYKIFSGTVVENITMFNRIYDSNAIDEILKQVDLYDDI
                     YSDELNIYSLVNRDGDNFSGGQKQRIAIARALYKNSEIILFDEPFSSLDKDNVDTLKE
                     IIEYLSITKTIIVVSHHNYLDDIADNVIQI"
     gene            4346..5002
                     /locus_tag="PLQ68_09125"
     CDS             4346..5002
                     /locus_tag="PLQ68_09125"
                     /inference="COORDINATES: protein motif:HMM:NF024570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4004 family protein"
                     /protein_id="HXK72854.1"
                     /translation="MNNDKELITKKELLIETGISYGQLYRWKREGVIPDEWFIKRSSF
                     TGQETYFKRKQILERIEIVKELRSKHSLEDLAKILSPDISEKIFLIDELKKIDEISSG
                     ILVSIKEVYDKKDFSLKEIVVFICVSKLQSECSLDVKQTSDILSDIKSCIADINNLDF
                     KMIILKLSDRLGTILYSSDKEIVISNELSVLAKYNLKEISEAVILKYREEKLHYFDEE
                     "
     gene            5010..5864
                     /locus_tag="PLQ68_09130"
     CDS             5010..5864
                     /locus_tag="PLQ68_09130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72855.1"
                     /translation="MMSSSNFGAGQSEYTGEYKKVRAAGSVKVFDCNIEDLFMSGDVN
                     ITNSNITKARVIGCVNAEKVVFDQGDILGHFTSTGICKAQIMHIAGVLNAEYFDAQIL
                     TDSKHFKNDKNATTVWSGNIKATTFESYHNFKVGPDCNFKNIINMNKILSGDKIYCNN
                     LISFSSIVTDLINADNIYITSVAGTKVKQIEGEYVWIGRQLPRHERITNIPKTLKNLI
                     ICKIRKIKKVEVDTIEADVIDIDYVKATKISGEIVNVGNNCIVDVVEYSKDLNLSKKA
                     IVKSVVKL"
     gene            5861..6454
                     /locus_tag="PLQ68_09135"
     CDS             5861..6454
                     /locus_tag="PLQ68_09135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72856.1"
                     /translation="MKNCKIQGSGRVTEGEYDNITIEGTGKLVDDVTVNTINVSGIMI
                     AKGKLRAKVIKSIGMIRLFKEASIDIIQIDKGVLTSKSDINSSLLECRGAIRIKGGIN
                     SDIVKIEGKGKVDYIVGENIIIRNNLQIENKVRHDKFKVNRIEGTSIEICNVNCRNIE
                     GDCIKVTGKSVVGQICYTKSLEMDPDSKVKRIVKLDR"
     gene            complement(6495..7034)
                     /locus_tag="PLQ68_09140"
     CDS             complement(6495..7034)
                     /locus_tag="PLQ68_09140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007061612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HXK72857.1"
                     /translation="MNSFYELASKRQSCRNYTDKPVEKDKLMKCINAAFLAPSAYNGQ
                     PWHYTLVINRETASKLAKTLQSGGMNKFTDKTPAFAVITQKASNASATIGGKLKHQDY
                     SSMDIGISTAHFVLQAEDLGLSTCIIGWFNEKDLKSLLSIKETDRIRLVIAVGYKDES
                     DKFRTKRRKPEDEVLKVIE"
     gene            complement(7093..8388)
                     /gene="gpmI"
                     /locus_tag="PLQ68_09145"
     CDS             complement(7093..8388)
                     /gene="gpmI"
                     /locus_tag="PLQ68_09145"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HXK72858.1"
                     /translation="MNNCVNNNSAMHFIGLFSDGNVHSNIAHLKAMLEQAKAEGVKKA
                     RIHILLDGRDVGETSALEYVEPFEEYLESLRSLNFDVKIASGGGRMNITMDRYEADWD
                     MVRKGWEIHVLGKGREFSSAKEAIETYRAETNVVDQFMPGFVITEANKPVGTIEDNDS
                     VIFYNFRGDRAIEISKAFEDSNFDKFDRVRVPNVIYAGMLEYDGDLHIPKNYLVSPPF
                     IKNTLGENLSKEGIKQYAISETQKFGHVTYFWNGNNSSKFKGETYVEVPSDNLPFEQR
                     PWMQCAQITDKLIDALKSGEYKFLRTNFPNGDMVGHTGSFDAAVIGVESVDLCLARIL
                     PVVDQMNGVAIITADHGNADEMFETKKGAVTIDKKTGKPKPKTSHTLNKVPFIIYDNR
                     EEKAYSLKDGEFGLANAASTVANLLGYEAPETWEESMIE"
BASE COUNT         3161 a         1255 c         1564 g         2928 t
ORIGIN      
        1 ttaagcccgt gaatacgaca caaatggaat ttagtctttc atttttttcg acttgattaa
       61 tactggaatt taagttttca ctttacgctt agttgagttt tcgtgttttg tattaggaag
      121 cgattatttg ccttcaattt tatgaagcta tgtctatctt cttatatagt gttcatttgt
      181 tgatgaccca gtcctcttct gcttctcctt ctcctcttcc tgttctttat acattgttat
      241 gttgctgttt tttaatatat gatatccttt gcaagactga gtatacatga tactgtcggc
      301 atacctgtcc ttataccatt cccaaagcta cattgaagcg aaatggagtt acttcatctt
      361 cctatatatg taatcgatct ccggttcagg cagtgcatcc atttcttttc cttgaagggt
      421 aaagcaggga tatcaaccac ttatcattta gttccatagg tcatgttgta ccagttcatt
      481 taactcgctc tattatttct gataatatta atatatcgca gttgaaagtc gatgtaattt
      541 ctcataatga gagcttaatt tcgtgattca aacgtaacta ctcataaatt ttcaatattt
      601 cctacttcct tatcgtatga tctctttctg tcacagcatg acataagagt atcagtattt
      661 aaatactatg acatagtgct cgaatgtttt cgaaatatgc aacaaacttc attcattatt
      721 tttcagagta atgggttaat ttcttttcat aagcccagtg cactttacct aggatggata
      781 ttgttactta ttatgtttat acaatattaa tagttataaa ttaatagaaa tttcgaaaat
      841 agctcttgac agcgtaacaa tgttatgtta cgctgtattt aaattctcag gtataaatag
      901 atattattaa aatctccaac atagtggaaa aaataactgg caatgagatt aagtggttgt
      961 gtaagtgatg attagtaatt atatcaaggg aatataacag aatgtaggga gaggaaaatg
     1021 ctatcgaatt tattcaaaaa ggttattgag gaaaacaaga gtgatgctgt tgtaggttta
     1081 ttttttggga ttattatagc tccagcagct ccaattttgt ttcaaattgg aatccagaaa
     1141 tttgttgact atacatttgg agataataat tatccaataa tatggatatt actagcttta
     1201 gttattctaa atataggttc attgtattca ggtaaattta tatataggaa aatcgaaaat
     1261 agactagatt taagttacaa aacaagtatt tcagaaatat ttactagaaa attaaagaga
     1321 gataacaatt tgtccagagg agaaataata cagaccattg aaatcataga tgatgtagtt
     1381 gatttcaaaa aagaaaaaat tatgtttctg cctttagtta tttcatttat attcattgtt
     1441 ctatatttta tcttcaattt aaatggttta tatctacttc aatacattct ttatgcaatt
     1501 cctataatta tcgtattaat cgtaatgact aatataatcg gtaagtacca aaaaagagtt
     1561 ttagaagaag ccggcaaagc caatgatata tctacggaaa ttattgaagg ttttgatatt
     1621 attacaaact tccgggcaac agatcatttt aaagatacat tttcaagaaa caacaattat
     1681 gtaaggaaat ttaaaaataa gattaatctt acagaaaaca taatcgacat tactaccata
     1741 ctaattaata tatctttaat tatcgcaatg ccattagcag ccagtttttt atacaaaagc
     1801 ggtaaaataa cggttggggg aatatttatt gcatcttcac tgattcaaca aattacagaa
     1861 attatttcga aaatagttgg cttttttgct tcaaacgcaa agaataagaa gtatatggaa
     1921 ttattaaata atattgagaa ctctgttgta agtacagaag gttgcaacac agcagaaatt
     1981 aataatttcg ataagttgta tattaaaaaa ttatcgttta cttatgatga taaaaatatg
     2041 atactagaca acttaagtta tgaatttgaa aaaaacaaca tatacataat taatggcgta
     2101 agcggtatcg gtaaaacaac gttaataaag ttaatagtta atgaacttga gattcagtct
     2161 ggagagataa cgctagataa tgttaatata agcccagaaa ttgttaaaga tttagtatct
     2221 tactcaagtc aacaatcatt tctttttcac gggaacatag aagaaaatat tgtttatggt
     2281 atcaatcagg atgtcaatga gactcaaaag aaagaactag ttgggaatct agagaaggta
     2341 tttaattttg ataatatttt caaaaaggaa aaagcgttag aaaacataga aataaatagt
     2401 cagagtgaca acatatcgtt gggtcagaaa aaactaatct gccttgcaag gaccattatt
     2461 aaagatgcaa aattagtgat tcttgacgaa cctacggcta atttaaatat cagacaagtg
     2521 gatattttta aaaacgaatt agtaaagcta aaagaagaca gaatcataat tgtaattacg
     2581 catgataaaa gattgaatga gattgcaagc agagtattaa gcatagttga cggggtaata
     2641 gtaaatgagc aataaaaaag ttccatatat aaacagtaca aaagagcaac tgataggaat
     2701 aggtttggaa ataattagcc agtcagctaa tgcattagct ttcataatat ttactttaat
     2761 tgcagggaat attattaatg gagtaataga tagtaattcg gtgttaatag aaagcacatt
     2821 gaaaaaagaa ttaccgatat gcttaatttt attagtaatt gtatatccaa tatttaaata
     2881 ttcagccaac atttataaga acaggaaaat atttgttctt aaggaatgca tgctggatct
     2941 gctgtttgag atgagtatta aaatacaaag taatcaattt ttgaataatt ttaatatgga
     3001 tataaacgac atagaaaaag ttattgagag aatatatccc ggcataataa acagtttatt
     3061 aacaagtata tatgccatag tattattgag tgtcggatat gatttcatgt ttgttataat
     3121 tttagtaacc accattatat ttaatttact gggcggttac ttcgcattaa gaaactttaa
     3181 gttggaagga gaaattaatg agaaaaaaag agatggtaac tctcatatat taaacatatt
     3241 cagtaataac ttaatattca atatatttcc aaatgctaga ttgtttttaa ataaaacaat
     3301 agatatacta acgaagatta gaaaagacaa agatatccag gcaaagaaca acacttacta
     3361 taatatatta ttttatgtaa gcgaagttat acgagttgtt ggtgtagtat atataggagc
     3421 aaggtataca gatataggga ttggaggtat tacatccttg cttttattaa ttgcacaatc
     3481 atcagtacta ttcagagatt ttagtgagaa tttactggga ttaaaaaagt tcagtattgc
     3541 aaaaaataag ctgagtgaaa catttttaat ggaaaatagt actacaaaag aaacagaagt
     3601 agaggtagaa agaaacataa atgatgaaaa aggtattgaa ttcagaaatg tattcttcag
     3661 ttacaattca ggaagcgatc tcttcaaagg tttatctttt aaagcgaata ataatgagtt
     3721 gactttaata aaaggtgata ttggaagagg taaatcaaca atgcttaaat tgatattaaa
     3781 gaaactgaat gtaaatgacg gtaatattta tattaatgga aaaagcatta atatattaga
     3841 tgattctgat ataaataaac ttattactta tgtagaccag ggatataaga tattttcagg
     3901 aacggttgtt gagaatatta ccatgtttaa taggatttat gattcaaatg caattgatga
     3961 aattttaaaa caggttgatt tatatgatga catatattct gatgagttga atatatattc
     4021 tttagtaaat agagatggtg ataatttttc aggaggacag aaacaacgta ttgcaatagc
     4081 cagagcatta tacaagaatt cagaaataat tctctttgat gaaccatttt cttctcttga
     4141 taaagataat gttgatacac taaaagaaat aatagaatat cttagtataa ctaaaactat
     4201 tattgttgtt tcacatcata attatttaga tgatattgca gataatgtaa tacagatatg
     4261 atgagaaact ccaatggcaa gaaccattta aatgcatttc ttgacaacgt aacaatgttt
     4321 gtttatgctg aagttggtga ttaatatgaa taatgataaa gaacttataa caaaaaaaga
     4381 attactaatt gagacaggta tatcttacgg acaactgtat agatggaaaa gagaaggagt
     4441 tattcctgat gaatggttta ttaagaggtc ttcctttaca ggacaggaaa catactttaa
     4501 aagaaaacag atacttgaac ggattgaaat cgtgaaagaa ttaagatcaa agcactccct
     4561 tgaggatctt gcgaaaatcc tttctcctga tatttctgaa aagatatttc tgattgatga
     4621 attaaagaag attgatgaaa tatcatcagg tattctagta agtataaaag aagtatatga
     4681 caaaaaagac ttttcattga aagaaatagt ggtttttata tgcgtatcta aactgcaaag
     4741 tgaatgctct cttgatgtaa aacaaacaag cgatatctta agtgatatta aaagctgtat
     4801 agcagatatc aataatcttg attttaagat gataattttg aagttatccg accggcttgg
     4861 tacgattcta tatagttctg ataaagaaat tgtaataagt aatgagttat cagttttagc
     4921 aaaatataac cttaaagaaa tttccgaagc agtaatcctt aaatatagag aagaaaaatt
     4981 acactatttc gatgaagaat gaggaaaaaa tgatgtcaag tagtaatttc ggtgcaggac
     5041 aaagtgaata taccggtgaa tataaaaagg ttagagcagc tggtagtgta aaggtatttg
     5101 attgtaatat tgaggattta tttatgtcag gggacgttaa tattactaac agtaacatta
     5161 ctaaggcaag agtaataggt tgtgtaaatg ctgaaaaagt ggtatttgac caaggtgata
     5221 ttttaggaca tttcacatct acaggtatat gtaaagcaca aataatgcac atcgcaggag
     5281 tattgaatgc tgaatacttt gacgcacaga ttttgactga cagtaaacac tttaaaaatg
     5341 ataagaacgc tacaactgtt tggtcaggga atataaaagc aacaacattt gaaagttatc
     5401 ataatttcaa ggttggaccg gactgtaatt ttaaaaatat aattaatatg aacaagatat
     5461 tgtcaggaga taagatttat tgtaataact taatatcttt ttcttcaata gtaactgact
     5521 taataaatgc tgacaacatt tatattactt ctgttgctgg aacaaaagta aaacagatag
     5581 agggcgaata tgtatggata ggaagacaat tacctcgaca tgaaagaata acaaacatac
     5641 caaagacact taaaaatcta ataatatgta aaataagaaa aattaaaaag gttgaagttg
     5701 atactattga agcggatgta attgatattg attatgtcaa agcaactaaa ataagcggag
     5761 aaatcgttaa tgtcggaaat aactgcatcg tggatgttgt tgaatatagt aaagacttaa
     5821 acctaagtaa aaaagcaatt gtgaagagtg tggttaaact atgaaaaact gtaaaataca
     5881 gggctcaggc agagtcacag aaggtgagta cgataatatt acaattgaag gtacaggaaa
     5941 actggttgat gatgttacag taaatactat caatgtgtct ggaataatga ttgcaaaagg
     6001 aaaactgaga gcaaaagtca taaaatcaat tggtatgatt aggcttttca aggaagctag
     6061 tattgatatt atacagattg acaaaggtgt tcttacaagt aaaagcgaca ttaattcttc
     6121 tttattggag tgcagaggag ctattaggat aaagggtggt attaattcag atatagtaaa
     6181 gatagaaggc aaaggtaaag tggattacat agtcggagaa aatattataa taagaaacaa
     6241 ccttcaaata gagaataaag taagacatga taagttcaaa gttaacagaa ttgaaggtac
     6301 ttcaattgaa atatgtaatg tgaattgcag gaatatagag ggtgattgta taaaggtgac
     6361 aggtaaatct gttgttggac aaatatgcta cacgaagtca ttggaaatgg atccagatag
     6421 taaagttaaa cgtatagtaa agcttgatag atagaagcat ttacctataa gtatttagaa
     6481 catgaaaaaa gccgctattc tattaccttc aaaacctcat cttcaggctt tcttctttta
     6541 gtacggaact tatctgattc gtccttataa ccgacagcaa taaccagcct tatcctgtct
     6601 gtctctttaa tggaaagaag cgatttgaga tccttctcat taaaccaccc tatgatacag
     6661 gtggataatc ctaaatcttc cgcttgcagc acgaagtgtg cagtagatat gcctatatcc
     6721 atggaggaat aatcctgatg cttaagtttt cctccaatag tcgctgatgc gttggatgct
     6781 ttctgagtta taacggcaaa agcaggggtt ttgtctgtga atttattcat tccgcctgac
     6841 tgtaatgtct ttgctaattt agatgctgtt tcacggttga taaccaatgt ataatgccag
     6901 ggctgcccgt tgtatgcaga aggagctaaa aatgcggcat ttatacattt catcaattta
     6961 tctttttcta ccggcttgtc tgtatagttc ctgcagctct gtctttttga tgctaattca
     7021 taaaaactgt tcatttatta tcctccttta atattaatag taattattta ttttttattg
     7081 gtttgttaag cgctactcta tcatgctttc ttcccaagtt tcaggtgcct cataacctaa
     7141 aaggttagca acggttgaag ctgcattagc taatccgaat tcaccgtctt ttaaagaata
     7201 agctttttct tctctgttgt catagattat gaatggaacc ttgttaaggg tatggcttgt
     7261 cttaggcttt ggttttccgg ttttcttgtc tattgttact gcgccttttt tcgtctcaaa
     7321 catttcgtct gcatttccat ggtctgcagt tataattgct acaccgttca tctggtctac
     7381 aactggcagt atccttgcta aacacaaatc aactgattct actcctatta cagcggcatc
     7441 aaagcttcct gtatgaccta ccatatcccc attagggaaa ttagtcctta aaaacttata
     7501 ctctccgctt tttaatgcat cgataagctt atctgttatc tgtgcgcact gcatccatgg
     7561 gcgctgttcg aaaggaagat tatctgaagg aacttcaaca tatgtctcgc ctttgaattt
     7621 ggatgagttg ttaccattcc agaaatatgt tacatgcccg aatttctgtg tttcagatat
     7681 agcatactgt ttaatgcctt cttttgacag attctcccct aatgtattct ttataaacgg
     7741 agggctgact aaataattct tcggtatatg aaggtctccg tcatattcca gcattcctgc
     7801 atatatgaca tttggtactc ttactctgtc aaatttatca aaattactgt cttcaaatgc
     7861 cttgcttatc tcgattgctc tgtctcctct gaagttatag aagatgacgc tatcgttatc
     7921 ctcaattgtt ccaaccggct tattggcttc tgttattaca aaacctggca tgaactggtc
     7981 tactacattg gtttctgctc tgtatgtctc tatagcttct ttggcagatg agaactcacg
     8041 gccttttcct aatacatgta tctcccagcc ttttctaacc atatcccagt cagcttcata
     8101 tctgtccatg gttatattca tcctgccgcc gccgcttgct atttttacat caaagttcaa
     8161 agaacgaagg ctttcaagat actcttcaaa aggttcaaca tattccaaag cagatgtctc
     8221 tccgacatct cttccgtcta gcaatatatg tatgcgtgct ttcttaacgc cttcagcttt
     8281 tgcctgttcc agcattgcct taaggtgggc tatattggaa tgtacattac cgtctgagaa
     8341 cagcccgata aaatgcatag ctgagttatt atttacgcag ttattgatag tgactaatca
     8401 gaggcttgag aaagtgaaaa tcgaaaaatg aagacaggtc ggtaaaagac agaactgatg
     8461 attgtaataa atgcaaatgt ctttgacata atataataat ttcttaaagg tattaaaatg
     8521 cttcattaga catcaccaca gcattatgca tgatagtaga atacactaaa tgcttcccat
     8581 aagcaagaca gtatatgaca agatccattt caagaagcta ttgctggatg gaatggatga
     8641 aattattcag gaagaacaag gaagacatat aggagtattt taagaacata tatacttgcg
     8701 cattttcaga gggaatgaaa agcatgacat agacacctaa gagggaaagc tgtatgagta
     8761 ttacatgata gcgatatact tatatattgt gtaattgcag ttctcatata cgaatccctt
     8821 ttcatcgcaa ctattcatga aataatgtaa aaaattaagt tgagttttcg tgttttgtat
     8881 taggaagcga ttatttgcct tcaatttt
//
LOCUS       DAOSXM010000084         8884 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2111
            NODE_5033_length_8884_cov_9.690339, whole genome shotgun sequence.
ACCESSION   DAOSXM010000084 DAOSXM010000000
VERSION     DAOSXM010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864178
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 8884)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8884)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:24:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,261
            CDSs (total)                      :: 2,229
            Genes (coding)                    :: 2,218
            CDSs (with protein)               :: 2,218
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 3 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8884
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5033_length_8884_cov_9.690339"
                     /isolate="AMR_MDS_2111"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..704)
                     /locus_tag="PLQ68_09150"
     CDS             complement(<1..704)
                     /locus_tag="PLQ68_09150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXK72859.1"
                     /translation="MWKFLVRRILLSIVILFFVSFIVYFIMRALPTSYVEAIARERAS
                     LPGGRSYTEWLQQLNQVYHLDGNVIEGFFGWLKDSIFTGDFGESWAYNVPVVQKFKDV
                     IWDSFILGFISLVLQLVISIPLGIIAARKQYSRLDYSISVFALIGISLPSFFFATLLK
                     LGFSYKLGWFEPIGKVSRFYDQLTPIGQFFDVASHYVLPIMTLTIVSIGFLMRYTRTN
                     MLEVLNSDYIRTARAKG"
     gene            complement(817..3252)
                     /locus_tag="PLQ68_09155"
     CDS             complement(817..3252)
                     /locus_tag="PLQ68_09155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015549835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXK72860.1"
                     /translation="MKLKTKSILSILLVVILCVAMLSACTPKIKKGEATILTPLVVAY
                     DPFSGKFSPFFADTAYDVDVAGMTQVGLMTTDRVGGVIYNAIKGETVNFNGTDYLYTG
                     VADLTVAYDEKSDTTTYRATIRNDVAFSDGTVMDIDDVIFTYYVLCDPAYTGSTTLSS
                     YNILGLQNYQTQTTDEVYDKYVTLVDAIYAAGEDHEWSASDAWTEAQQDGFWALLNEE
                     WKNDVGAIVNYVIGNYLSYADQGYFPYSSAEISASEGMKIAFGMRMWGFAGVEGTTLT
                     TAVTGKTFDMAAGEFPTLEDYFLETKEAYGGNPEEYWAVEAADDTDVVTPAKSKFIYE
                     YGSKDEAMGTDGIKSITGIKRVNNRTVEVTTKGYEASAIYSILGISITPMHYYGDESL
                     YNYKDGSYGFPFGDLSMVQAKTSNPMGAGPYKFVRYEDKVVYFEANEYYYKGTPKVKY
                     IQFKEVGESDKISSIASGAVDVANPSGSKVKFNEIRSYNSNGQLDGNVITTSSVDNLG
                     YGYVGINADTVRVGTDSDSAESKALRKGLATLLAVYRDVAINSYYGDAAAVINYPISN
                     TSWAAPQKSDADYKVAFSVDVDGNDIYTADMDAEARYAAALEASIGFFKAAGYTWNDS
                     TKKFTAAPEGANMTYEALIPGDGAGDHPNFQVLTNTSEALKKIGITLEVNDLADSSVL
                     WDRLDAGTQELWTAAWGSTIDPDMYQVYHSSGIVGKGGSDSNHYHIADSELDDLIVQA
                     RKSDDQTYRKSVYKACLDLIIDWAVEVPSYQRQNIIVFSTERVNIDTVTPDITTFWGW
                     MNDVELLEVYEAN"
     gene            complement(3477..4823)
                     /gene="glmM"
                     /locus_tag="PLQ68_09160"
     CDS             complement(3477..4823)
                     /gene="glmM"
                     /locus_tag="PLQ68_09160"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010963806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HXK72861.1"
                     /translation="MGKIFGTDGARGIANKDLTVSLAFNLGKAGAFVLAGECSHTPKI
                     LLGADTRISSDMLMSATEAGICAMGANVIRAGVVTTPAVAYLTKLYNCDAGIVISASH
                     NSYEYNGIKFFNKDGFKLPDETEDQIERIVTGEGGGSIKTHDAIGRIFKDYDCVKEYS
                     DFLVSHCNTDLSGLRILLDCANGAASNIAPEVFRRTGCDISYMNADPDGININKDCGS
                     THIDNLAKEVVKRNYDIGFAYDGDADRLLAVSEKGDLIDGDVLMAVLALHLKENGLLA
                     KDTITVTEMSNMGIDVFARKNDMQIVRTKVGDRYVVEEMLKSGYSIGGEQSGHIILLP
                     FNTTGDGILASLKISQILAENKIKASSFFDIMKPLPQVLINVRIRNDMKAELKNDVEI
                     QNLYKSISEELDGNGRIVLRVSGTEPVIRVMIEGENMEFIETRAKTVADKIKEKLN"
     gene            complement(4840..6417)
                     /locus_tag="PLQ68_09165"
     CDS             complement(4840..6417)
                     /locus_tag="PLQ68_09165"
                     /inference="COORDINATES: protein motif:HMM:NF013314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HXK72862.1"
                     /translation="MGKFILKEISVPVREAYNADIKSIIEQKVRKSYDNNDINFVSFQ
                     KKSIDARHKNNIQYSINAVFESDDDFIAKKKLEPFSSFEYEAIKMGSTKVRRPVVIGA
                     GPCGLFAAYLLALYGFEPLVIERGKCVDDRHADILEFFNTGHLNTESNIQFGEGGAGA
                     YSDGKLVTRINDPRCSYVLSVLKEMGADESILYDAKPHVGSDVLKNVVKTLRNEIISK
                     KGSFLYESKVTDLVLSKDMKIEGVIINDSTQIDANIVILATGHSARDTYQMLFAKGME
                     MIQKPFSVGYRIEHLQEDINECRYGKSAKYIPYNAEYQLHDRSQIRGVYTFCMCPGGY
                     VINASSEEKCLATNGMSMKKRDGINANSAWVVSVDNNDYLSDHPLAGIEFQRSIERKA
                     YEIGIANYVAGVQSLKGFLEKQPKRSNNIKPTIKPGYTYSDLSGIYSNEIYETLCSSV
                     SLFEKKLSCFSKGVLTACETRTSSPVSIIRDENHMSSTIYGIYPCGEGAGKAGGIMSA
                     AIDGLKTAENIIKNFRI"
     gene            complement(6430..7935)
                     /locus_tag="PLQ68_09170"
     CDS             complement(6430..7935)
                     /locus_tag="PLQ68_09170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72863.1"
                     /translation="MWNKIKKNLFFKILAVVIAIIIWFIVSVNNNPVEVKTISVPIQI
                     QNERNLALRNLRIINEIDNFIDIQIKGAATEVGKVSSSDFSAYVDFTEIINENYNNLT
                     VKGITYNGDSNITFTYDEEDIVKYVEVEKVITSEFPVSIDFNGQLPEGYYLVAYSVTP
                     NIQSINDVTSMISKIDSIKVSIDLDNVYQSFTVRKACNVYDKDMRILNEYSNTITVDV
                     SITIGKKVNLISRITGTDALGDNHMYISNSLQYSDLIIVGDNLVIKDINTVYTKPFDI
                     SGSKESFVSESELDIPSNIKAYSVDNVLISNNMVEISVTIEQLITKRFEYGIEEITIR
                     NKSILQEYTMNEEVFFVTLKGRAVTLASITKNQLTPYVDVFNMNDGNNPAKVNVLSVG
                     TNVTLEDSSTLNVYVETIATYSIDTEKIIKTNVNNELSYSIQAESFNIRLVGLSSNIA
                     EVDTSELAFYINMEDYQEGMHMIDVVVSNESLPEGVRLYDEITIAVMITKQ"
     gene            complement(7925..8800)
                     /gene="cdaA"
                     /locus_tag="PLQ68_09175"
     CDS             complement(7925..8800)
                     /gene="cdaA"
                     /locus_tag="PLQ68_09175"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003519140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HXK72864.1"
                     /translation="MFNQIDWLEQIKYFRLELSSPLAVISAIIDIAIVSYLVYKLMIF
                     VKEIRAWSLLKGVLVIIIVAIVSSLLGLQIMTFLFSNILTFAALSIVVLFQPEIRKVL
                     ERLGTSEFTHFFSSTGNDETLKTTAVVEEIVKACKNMSEQYIGALIVIEKETKIGEFI
                     NTGVQMNADVTKELLENIFVPKTPLHDGAVIIRKDKIIAAACLLPLTDNPNLSTELGM
                     RHRGGLGITEVSDCIAVIVSEESGKISYASNGGLVRNLNSDSLRKALLMNLVKEKKEK
                     SKLGKIIGKGKKNVE"
BASE COUNT         2664 a         1811 c         1275 g         3134 t
ORIGIN      
        1 ccttttgctc ttgcagttct aatataatct gaattaagta cttcaagcat atttgttctt
       61 gtatatctca ttaaaaagcc tatgcttact attgtcaaag tcataattgg cagcacataa
      121 tgcgatgcca catcaaaaaa ctgtcctatg ggagttaact gatcgtaaaa ccggctaacc
      181 tttccaatag gttcaaacca tcctaatttg tatgaaaacc ctagttttaa tagtgttgca
      241 aaaaagaatg acggtaatga aatacctatt aatgcaaaca cactgattga gtagtccaat
      301 ctgctatatt gtttccttgc agcaattatt cccaatggta tagaaataac taattgaaga
      361 actaaagaaa taaatccaag tataaatgaa tcccagataa catctttaaa tttctgaact
      421 acaggtacgt tgtatgccca agattcacca aaatcgcctg taaaaattga atctttaagc
      481 catccaaaaa atccttctat cacgttacca tccaaatggt acacttgatt taactgttga
      541 agccattcag tatagcttct tcctccggga agtgacgcac gttcccttgc tattgcttca
      601 acatatgatg taggtagtgc gcgcattata aagtatacaa tgaacgaaac aaagaaaaga
      661 atcactatac ttaataatat acgtctaact aaaaacttcc acattaatgt ttctccttac
      721 taaaacatgc ttacttcgca tagtttaaat attattatat acataaggaa agcccaccgt
      781 caacagcggg ctttcctttt tttttaggat tttgtactaa tttgcttcat aaacttcaag
      841 aagctcaaca tcattcatcc agccccagaa agttgtgata tcaggtgtaa ctgtatcaat
      901 attaactctt tctgtactga acactataat gttctgtctc tgatatgaag gtacttcaac
      961 tgcccagtca attattaagt caaggcatgc tttgtataca gattttctgt atgtctggtc
     1021 atctgactta cgtgcttgaa caataagatc atcaagttca gaatcagcta tgtggtaatg
     1081 gttggaatca gatccgcctt tacctactat tcctgagctg tgatatacct gatacatatc
     1141 aggatctatc gttgagcccc atgcagctgt ccacaattcc tgtgttcctg catcaagtct
     1201 gtcccaaagt actgaagaat ctgctaaatc gttaacttct agtgttattc caattttctt
     1261 taaagcttca gaagtatttg ttaaaacctg gaagttcggg tggtcaccag cgccatcacc
     1321 aggaatcaaa gcttcataag tcatatttgc tccttcagga gctgcagtaa actttttagt
     1381 tgaatcattc catgtgtatc ctgctgcttt gaagaatcca attgatgctt caagtgctgc
     1441 tgcgtatctg gcttctgcat ccatgtctgc tgtgtagata tcattaccgt caacatcaac
     1501 tgagaatgca actttatagt ctgcatcaga tttctgaggt gcagcccagg aagtattgga
     1561 tataggatag ttgataactg ctgctgcatc gccataatag ctgtttatag ctacatcacg
     1621 atatactgca agaagtgttg ctaaaccttt tctcagagct tttgattctg ctgaatcgga
     1681 atctgttcca actcttactg tgtctgcatt aatacctaca tatccataac ctaagttgtc
     1741 aactgatgat gttgtaataa cattaccatc aagctgtccg ttagagttat atgaacgaat
     1801 ttcgttaaac ttaactttac ttccgcttgg atttgctaca tctactgctc cagaagctat
     1861 tgaactgatc ttatcagatt cgcctacttc tttgaactgg atgtatttaa cttttggagt
     1921 tcctttatag taatattcgt tagcttcgaa ataaacaact ttatcttcat atcttacgaa
     1981 tttataagga cctgcaccca taggattgga tgtctttgcc tgtaccattg aaaggtcgcc
     2041 aaatgggaat ccatagcttc cgtctttgta attataaagg ctttcgtctc cataatagtg
     2101 cattggggta attgatattc caagtattga atatatagca gaagcttcat atcctttagt
     2161 tgtaacttca actgtacggt tattaactct ctttatacct gtgatgctct tgattccatc
     2221 agttcccata gcttcgtctt ttgatccata ttcatatatg aattttgact tagcaggtgt
     2281 aactacatca gtatcatcag cagcttcaac tgcccaatac tcttcaggat ttccgccata
     2341 tgcttctttg gtttctaaga aataatcttc aagtgttggg aattctccag cagccatatc
     2401 aaatgttttt cctgttacag ctgttgtaag tgttgttcct tcaactccag caaatcccca
     2461 cattctcatt ccaaaagcaa ttttcattcc ttcacttgct gatatttcag cagatgagta
     2521 tgggaaataa ccctggtctg catatgaaag atagtttcca ataacatagt ttactattgc
     2581 tcctacatca tttttccact cttcgttaag caatgcccag aatccgtctt gttgagcttc
     2641 tgtccatgca tcagatgctg accattcatg atcttctcct gcagcataaa tcgcatcaac
     2701 taatgtaaca tacttgtcat aaacttcatc agtagtctgt gtctgatagt tctgtaatcc
     2761 aagaatgtta tatgatgata aagttgtcga gccggtgtat gcaggatcgc aaagaacgta
     2821 atatgtgaat attacatcat caatatccat tacagtaccg tctgagaacg ctacatcatt
     2881 tctaatagtc gcacgatatg ttgtagtatc tgatttctcg tcatatgcta ctgttaaatc
     2941 tgcaacacca gtgtaaagat aatcggttcc attaaaatta accgtctctc cttttatggc
     3001 attgtaaata acaccgccaa ctctgtcagt agtcataagg ccaacctgtg tcattccagc
     3061 aacatctacg tcatacgctg tgtctgcaaa gaatgggctg aacttgccgc tgaaaggatc
     3121 gtatgcaaca actaacggtg ttagtattgt cgcttcgccc tttttaatct taggcgtaca
     3181 agctgacagc atggctacgc aaagaataac tacaagcaat atgctgagta tgctctttgt
     3241 ttttaacttc ataattttac ctcctcataa tagtaaatct ttaataataa agttttaaat
     3301 ataatatcat ggtcatgaaa aagtgtcaac gcattttttc atattctgtt cacattcttg
     3361 ccacattttg ttttcaatgt taatattctc tatttaaaag gcagaatatc ataattctgc
     3421 caaaagaata aaaacataat atgaataaag ctaatgcaaa atattcaaat tactaattaa
     3481 ttcagttttt cttttatctt gtcagctacg gtttttgctc gagtttcgat aaattccatg
     3541 ttctctccct cgatcattac tcttattaca ggttcagtac cagatactct caaaactatc
     3601 cttccatttc catccagttc ttctgatatg ctcttatata aattctgtat ctccacatca
     3661 tttttaagtt cagctttcat atcatttctt attcttacat taatcaatac ctgcggtaat
     3721 ggtttcatta tgtcgaaaaa actgcttgct ttaattttat tctcggctaa tatttgtgaa
     3781 attttcaaag aagcaagtat tccgtctcca gttgtattaa atggtagaag tataatatgt
     3841 cccgattgtt ctccaccaat ggaatatccg ctttttagca tttcttctac tacataacga
     3901 tctccaactt ttgttctgac aatttgcatg tcattttttc tggcaaatac atcaattccc
     3961 atattactca tctctgttac agtaatagtg tcttttgcca gtaatccgtt ttctttcaag
     4021 tgaagtgcca agacggccat cagcacatca ccgtctatta aatctccttt ttccgagaca
     4081 gctaatagcc tgtcagcatc accgtcataa gcgaatccga tatcataatt tctcttaact
     4141 acttcctttg ccagattatc gatgtgagtg gacccgcagt ctttgttaat attaattcca
     4201 tctggatctg cattcatata tgagatatca catcctgttc ttctaaatac ttcaggtgct
     4261 atattgcttg cagctccatt tgcacagtct aaaagaattc tcaaaccgga taagtcggtg
     4321 ttacagtgag atactaaaaa atcagaatat tcctttacac aatcataatc tttgaaaatc
     4381 ctgcctattg catcatgggt ttttatactt cctccaccct cccctgtaac tattcgctca
     4441 atctggtctt cagtctcatc aggaagctta aacccgtctt tattgaaaaa ttttatacca
     4501 ttatactcat acgagttatg tgaagctgaa ataactattc ccgcatcaca gttatacaat
     4561 tttgtaaggt atgcaacagc tggggtagta acaactccgg ctctaatgac atttgctccc
     4621 atagcgcaaa ttcctgcttc tgtagcagac ataagcatat ctgatgaaat cctagtatct
     4681 gctcctaaaa gtatttttgg cgtatgactg cactcacctg ctaatacaaa agctcctgct
     4741 ttaccaagat taaatgcaag gctgactgtt aaatccttat ttgcaattcc tcttgctcca
     4801 tctgtaccga aaattttacc catttaatcc tcctcaaccc taaatcctga aatttttaat
     4861 tatattttct gctgttttaa gaccatcaat tgctgctgac ataatacccc ctgcttttcc
     4921 agcaccctct ccgcaaggat atataccgta aattgtgcta gacatatgat tttcgtctct
     4981 tataatactt accggagagc ttgttcttgt ttcacatgcc gtcagtaccc ctttactaaa
     5041 acatgatagt ttcttttcaa acaaggatac tgaagaacat aaagtttcat atatttcatt
     5101 actatatata ccacttaaat cggaatatgt ataccccggt ttaattgtag gtttaatatt
     5161 atttgacctt tttggctgtt tttcaagaaa tcccttaagg gattgtacac cagctacgta
     5221 attagctata cctatttcat aagcttttct ttctattgat ctttggaatt ctattccagc
     5281 aagcggatga tccgacaggt aatcattatt atcaacagat actacccatg cgctattagc
     5341 attaatgccg tctctctttt tcatgctcat accatttgta gcaagacatt tttcttcaga
     5401 agatgcatta atgacatatc ctcccggaca catacaaaaa gtatatactc ctcttatctg
     5461 tgatctgtca tgtagctgat actctgcgtt atatggtata tattttgctg atttgccata
     5521 tctgcattca tttatgtcct cttggagatg ttctattcga taacctactg aaaaaggttt
     5581 ttgtatcatt tccatacctt ttgcaaaaag catctgatat gtatctctag cactgtgtcc
     5641 tgttgcaagt attacaatat ttgcatctat ctgggtacta tcgttaataa tcacgccttc
     5701 tattttcata tcttttgata aaaccaaatc agtaactttt gattcataaa gaaaagaacc
     5761 ttttttcgaa ataatctcat ttcttagcgt ttttacgaca ttttttaata catctgaacc
     5821 gacatgcggt tttgcatcat ataaaattga ttcatcagcg cccatttctt ttaaaacaga
     5881 taagacatat gagcatcttg gatcattaat tcttgtaaca agcttcccat cagagtatgc
     5941 tcctgctcca ccctcgccaa attgtatgtt agattcggta ttaaggtgtc cagtgttaaa
     6001 aaattccaat atatcagcgt gcctgtcatc tacacattta cctctttcga taactaatgg
     6061 ttcaaatcca tataatgcca aaagatacgc tgcaaataac ccgcaaggtc cggctccaat
     6121 aacaacaggc cttcttactt tggtacttcc catttttatc gcctcatact caaaagagga
     6181 aaatggctct agtttctttt ttgcgataaa atcatcgtct gactcaaaaa cagcattaat
     6241 actatattga atattgtttt tatgccttgc atcaattgat tttttctgaa aacttacgaa
     6301 atttatgtca ttattatcat atgacttacg aaccttctgt tctatgatac ttttaatatc
     6361 agcattatat gcttccctta caggaacgct tatttctttt aaaatgaatt ttcccatgat
     6421 tccttcaatt tattgctttg ttatcataac agcaattgtt atttcatcgt atagcctgac
     6481 accttcaggc agactttcat tacttacaac tacatcaatc atatgcattc cttcttgata
     6541 atcttccata tttatataaa atgcaagttc cgatgtatca acctctgcta tattcgaact
     6601 caatcctact aaacgtatat taaatgattc tgcctgtata gaataactaa gttcattgtt
     6661 tacattagtt ttaataatct tttctgtgtc tatagaatat gttgctatag tctcaacata
     6721 aacgttcaat gttgaagaat cttccaaagt aacattagtt ccaacagata gaacatttac
     6781 ttttgcaggg ttgtttccat cgttcatgtt aaaaacatca acatatggcg ttaattgatt
     6841 cttggttatt gaagctagtg ttacagctcg tcctttcaat gtaacaaaga acacttcctc
     6901 attcatagta tactcttgaa ggatactctt attccttatt gtaatctctt caattccata
     6961 ctcaaatctt tttgttatca gttgttcaat agtaaccgat atttcaacca tgttattaga
     7021 aataagtaca ttatcaacag aataagcttt tatatttgaa ggtatatcaa gttctgattc
     7081 acttacaaat gattctttgc ttcctgaaat atcaaaaggt tttgtataaa ctgtatttat
     7141 atcttttata accaggttat ctccaacaat aatcaaatct gaatattgaa gagaattaga
     7201 aatatacata tggttatccc caagagcatc agtgcctgta atacggctta tcagatttac
     7261 tttcttgcca atagtaatac tgacatcaac agttatcgta tttgaatatt catttaaaat
     7321 tcgcatatcc ttatcgtaaa cattacaagc ttttcttact gtaaaagact gataaacatt
     7381 atccaaatca atacttactt ttatactgtc aatcttcgat atcattgaag tgacatcatt
     7441 tatagactga atatttgggg taacagaata agcaacaaga taataacctt ctggaagctg
     7501 cccattgaaa tctattgaaa caggaaattc agatgtgatt actttttcaa cttcaacata
     7561 tttcacaata tcctcttcat catatgtaaa tgttatattt gaatcaccgt tatatgttat
     7621 tcctttaact gtaagattat tataattctc gttgattatc tctgtaaagt ctacatatgc
     7681 agaaaaatct gaagaagaaa cctttcctac ttcagttgct gctcctttaa tttggatatc
     7741 tataaaatta tctatttcat taattatcct tagatttcga agagcaaggt ttctttcatt
     7801 ttgtatttgt ataggaactg atatagtttt tacttcaaca ggattattat taacagaaac
     7861 aataaaccaa ataataatgg ctataactac tgctaatatc ttgaaaaaaa gattcttttt
     7921 aatcttattc cacatttttc ttaccttttc ctataatttt ccctagtttg cttttttctt
     7981 tcttttcttt aacaagattc attagtaagg cttttctaag cgaatcactg ttgagatttc
     8041 ttaccagtcc gccatttgaa gcatatgaga tttttccgct ttcttccgaa acaataactg
     8101 caatacaatc ggatacttcc gttataccta atcctccacg gtgcctcatt ccaagctctg
     8161 tacttaaatt tggattatct gtaagtggca ataagcatgc tgctgcaata atcttatcct
     8221 tcctgattat aacagctccg tcatgcaatg gagttttcgg aacgaatata ttctctaata
     8281 gctctttggt aacatcagca ttcatctgaa cacctgtgtt tatgaattcg ccgattttgg
     8341 tttccttttc aataactatt aatgctccaa tgtattgttc agacatgttt ttgcatgctt
     8401 ttacaatctc ttcaacaact gctgtagttt taagagtctc atcattaccg gtacttgaaa
     8461 aaaagtgagt gaattcgctt gttccgagtc tttcaaggac ctttctgatc tcaggttgaa
     8521 aaagaacaac aatagacaaa gcagcaaatg tcaatatatt actaaaaaga aatgtcataa
     8581 tctgcaaccc tagcaaactg ctaactattg caacaattat gatgacaagt actcctttaa
     8641 gcaagctcca tgctctgatt tcttttacga atatcataag tttataaaca agataagaaa
     8701 caatagctat atcaataatc gctgatataa cagcaagtgg actggacaat tccaacctga
     8761 agtatttaat ttgctctaac caatctatct gattaaacat tgctctttcc tgtttcagcc
     8821 tttttttcga tatattacaa ttatacagta tcttgttata ttatgtatac attattcaac
     8881 tgtt
//
LOCUS       DAOSXM010000085         8822 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2111
            NODE_5078_length_8822_cov_10.098551, whole genome shotgun sequence.
ACCESSION   DAOSXM010000085 DAOSXM010000000
VERSION     DAOSXM010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864178
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 8822)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8822)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:24:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,261
            CDSs (total)                      :: 2,229
            Genes (coding)                    :: 2,218
            CDSs (with protein)               :: 2,218
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 3 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8822
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5078_length_8822_cov_10.098551"
                     /isolate="AMR_MDS_2111"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            310..>1871
                     /locus_tag="PLQ68_09180"
     CDS             310..>1871
                     /locus_tag="PLQ68_09180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HXK72865.1"
                     /translation="MKKKNFSIKKMIISVFMGFLIIAISAHGILIFQNWMASSKRTTE
                     DIANQMNTQINEQINFYMYMPYYINETSHKIIQNGILDMYDEVARNKFFVGVLMSNPT
                     GIYSFSFGTAEGEYYGARRNEAGQIEIMKNDFSTGNESWYYSINDDMTMDELVVKAGK
                     FDPRTRAWYQSAVDVKGFTFSPIYKHFIMDDIAISASWPVYDNNNELSGVIGTHMLLS
                     DLNNYLINAVESYNGYALIVENGTNNLIGNSMGLKNYTTLSDGTLNRTNITDIEIEDI
                     QKGHEQYLKENNSDFYFDGTDDNFYISIQNIKLEGVDWTVMSAIPESVLMKDITNSMI
                     WAGSVAIFVILLILITYVLMTNRLLKPMNDLLESAYAFSNGELSARIKIKRNDEIGLI
                     SESFNNVADKMQSLVENLEENVRQRTDELRLILDSAAEGIYGVDLEGNCTFCNMSCLK
                     ILGYEKQEDLIGKNVHKIIHHSYIDGTEYLTEDCLILKSIRNGKGYENSEEVFWKADN
                     TYFNVDYSAYPQV"
     assembly_gap    1872..1971
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1972..2841
                     /locus_tag="PLQ68_09185"
     CDS             <1972..2841
                     /locus_tag="PLQ68_09185"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HXK72866.1"
                     /translation="HNVGDELIKKSAEVLKKACRKEDIISRVGGDEFIILLPKTEKDI
                     AEQLTRLIKAEMSNSKVAAIRCSMALGVNTKTQERQVLDEIIADAENAMYKDKTMNRQ
                     STNRDLINTIIETLHSRSPQEKQHSDNVQKLCSMMGKALKLSKPEIDKLERSAYLHDI
                     GKITIDKEILSKKELSEEEKEKMYQHPVVGYRILNLFDETLDLAEYIYNHHERWDGLG
                     YPRGVAKDEIPFISRIIAVVETYDRIVYGYSAIRDKDSKINKERAISIIEEGSGKQFD
                     PKIVKVFLEIMLK"
     gene            2893..4152
                     /locus_tag="PLQ68_09190"
     CDS             2893..4152
                     /locus_tag="PLQ68_09190"
                     /inference="COORDINATES: protein motif:HMM:NF025774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MEDS domain-containing protein"
                     /protein_id="HXK72867.1"
                     /translation="MKDLFIQSGEVRPGDHLVALYKDEKEIFDYVTAYIHSALARNES
                     CIYITGDVDTSMVLNEIRNMTEVSHAKGDLLILDRTETYSKEGKFSPDRLINMIKDMV
                     KTALDDGYTALAITGEISWVLDYEDGEQLIIEYEWKLNEYIFNTYPVSALCRYNINRF
                     SYEMVKNIIQLHPILIWNNKIHENPYYIPAEGFKNNEIAKYQVDVWLKNIFNFTATKK
                     RFMTVLEKSQQEMQKLHKNMTNGIIMAFLNLLENHDTYTKDHSTNVALLSSKIADSLN
                     ESEEFKTKIYYVSLVHDLGKALIPKDILNKPGKLSDEEYGYIKLHPIYGANALKQMDQ
                     LGDISLAVRHHHERYDGKGYPDGISGDNIPLMSRIIALCDSYDAMINDRPYRKANSHE
                     YAINEIIACSGTQFDPVLVDHFVKLFS"
     gene            4237..4437
                     /locus_tag="PLQ68_09195"
     CDS             4237..4437
                     /locus_tag="PLQ68_09195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015534972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HXK72868.1"
                     /translation="MNTIGKYIKQKRKEAGLTQEEFAIRSGLGLRFVRELEQGKETVR
                     MDKINQALSMFGMETVPGKRDE"
     gene            4437..4769
                     /locus_tag="PLQ68_09200"
     CDS             4437..4769
                     /locus_tag="PLQ68_09200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007883533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA N-terminal domain-containing protein"
                     /protein_id="HXK72869.1"
                     /translation="MHDPYRTAYIYIQDIYAGILKETDTGYSFQYDKDYLSNPEASSA
                     SLTLPIQEKAFFSKTLFSFFDGLIPEGWLLDIVTHNWKININDRFGILLIACKDCIGN
                     VSIREIKE"
     gene            4766..5737
                     /locus_tag="PLQ68_09205"
     CDS             4766..5737
                     /locus_tag="PLQ68_09205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015534970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA domain-containing protein"
                     /protein_id="HXK72870.1"
                     /translation="MNCLCCGKPLSDDSAVEKASFWHNSCIKKFFFANRLPIIDVSEQ
                     MLMHLALENTKKGFTIPGVQKKISLHLTQGNNPRLTLVNYPTGYILKPQVSEYHSLPE
                     AEFLVMQMALKTGINTVPFALLMMEHNNYSYITRRIDRILPTGKNIKTQMLAMEDFCQ
                     LSGRLTVDKYHGSYEQCAKIISKYSINTGLDLSELFVRIVFSYVVGNSDMHLKYFSLI
                     ETAPNSQEYVLSPAYDMLPVNIILPDDKDQLALTLNGKKRNIRKNDFLFFAENSRIPQ
                     KSAEKMIKKVVSLKETYLKMCRDSYLPDDMKSVFEKLITDRISVLTE"
     gene            5922..6932
                     /locus_tag="PLQ68_09210"
     CDS             5922..6932
                     /locus_tag="PLQ68_09210"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HXK72871.1"
                     /translation="MNSREKVKNIFLRKSNGAGAMWTGHPNDKTIPIYAKKWNIEPSR
                     EAIFTYLDDDCRWVIANSGYKHPQGLADFNPAWNTKRDTLSAEGCFADAENISDIEKY
                     PWPDVKYCDFTDIYAQIDKFPDKMIFTGIWSHFFHLVADFFGMENYFIKMYENPKIVE
                     AVTERIVDYCVEANDLFFKGLGDRADAMFFGNDFGTQQDLFLSPEMFRKFVMPSFKRL
                     IQIGKKYNKIIILHSCGSIYRIIPDLIEAGVDVLHPIQAQAVGMSAKDLSIYKNDLAF
                     LGGIDAQTFFVNATPSQIKDEVRRVKSILGPNIVISPSHEEILPNVPAENILAMAEAA
                     RE"
     gene            complement(6907..8508)
                     /gene="metG"
                     /locus_tag="PLQ68_09215"
     CDS             complement(6907..8508)
                     /gene="metG"
                     /locus_tag="PLQ68_09215"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015391934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HXK72872.1"
                     /translation="MNILIGGAWPYANGSLHIGHIAGLLPGDIIARYYRAKKDNVFYV
                     SGTDCHGTPICIKAKKENTSPQQICDKYHNEFTEVFNRLGFSYDLYGKTTDKWHIDFV
                     QNFHKELYKSKYVYELDSPSAYCNNCDKPLTDRLVIGTCPICGNETNGEQCDFCTTVN
                     DVTSIKNPICSECKNPVFFYNTKHLYIAISKLENQLRKYLDKHNNWRKNAVAFTKRYI
                     DEGLKDRAITRDLEWGIEVPKQGYEKKRIYIWAENVLGYLSASKVLCEKKDIPFSALW
                     KDNSRHYYVHGKDNIPFHTIILPSLLLAHNQYTHLPDDIISNEHLTLEGRKISTSKNW
                     AIWAKDIVEKYNPDSIRYFLTANGPEKRDTDFTWREFTERNNSELLGTYGNFVNRTLL
                     FTKKYQNSIVPKGVSDIAINKLIENTFIGVSGKIEEGRLKEALEEIFSLIRFGNKYYD
                     TLEPWKTRLNNQKECDNAIYNCTQIIANAAVLLKPFIPFSSEKVQSWLNLDDSWSIKK
                     VEAGYTLPEISILFERISIPSQPLP"
BASE COUNT         3015 a         1274 c         1643 g         2790 t
ORIGIN      
        1 tcataccaga aagaacttat tgtttatcca aaacttaata agaacagacg ctatatgctg
       61 gataatgaaa tatataaatc agaacaatta atggaaaacg gaatcagaat gaaatttagt
      121 gaatcaacaa gaacaagtga gattgtatta aaatctgtca tttaagtaca ggctagggtg
      181 gttagtgtaa gatttattaa cactgaatat gatataatta cattgatata cgcatttgat
      241 aaaatgtgaa ttctgtatag caaatgttaa atggggcagt ttgaaaataa ttgaattaag
      301 gattgggaaa tgaagaagaa aaacttttct attaaaaaga tgataataag tgtttttatg
      361 ggttttctca taattgcaat ttcagctcat ggtattttga tattccaaaa ctggatggct
      421 tcttcaaaaa gaaccacaga agatatagca aatcaaatga atacgcaaat taatgagcag
      481 ataaatttct atatgtacat gccgtattat ataaatgaaa caagtcataa gataatacag
      541 aatgggattc tggacatgta tgatgaagta gcaagaaata aattctttgt tggcgtgctt
      601 atgtctaatc caaccggtat atacagtttc agttttggaa cagctgaagg tgagtattat
      661 ggagcacgaa gaaatgaagc tggacaaata gaaattatga agaatgattt ttctactggc
      721 aatgaatcat ggtattactc aattaatgat gatatgacta tggatgagct tgttgtgaaa
      781 gctggaaaat tcgatccaag aaccagagcc tggtatcagt ctgcagtgga tgtaaaagga
      841 tttaccttct ctccgattta taagcacttt attatggatg atatagcgat ttccgcatca
      901 tggccagttt atgacaataa caatgaattg tcaggagtaa taggaactca catgcttctt
      961 tctgatttga ataattatct tataaatgca gttgagagtt ataacggata tgcgttaatt
     1021 gttgaaaatg gtacaaataa tcttataggt aattctatgg gtttgaaaaa ctatactaca
     1081 ctatctgacg gaacattaaa tagaacaaat attacggaca ttgagataga ggatatacaa
     1141 aagggtcatg agcagtattt aaaggagaac aattccgatt tctattttga tggtacggat
     1201 gacaatttct atataagcat tcaaaatata aaacttgaag gtgttgactg gacagttatg
     1261 tctgcgatcc ctgaatcggt tctaatgaag gatataacaa acagtatgat atgggcaggt
     1321 tctgttgcaa tatttgttat actccttata ttaataacgt atgttctgat gacaaataga
     1381 ttattaaagc ctatgaatga tttattggaa tcagcatatg ctttctcaaa tggggaattg
     1441 tcagcaagga taaagataaa gaggaatgat gaaataggat taatatctga aagctttaac
     1501 aatgtagctg ataaaatgca gtctcttgtt gaaaatcttg aagagaatgt aagacagaga
     1561 accgatgaac ttcgacttat actggattca gcagctgaag gaatttacgg agtagacctt
     1621 gaaggtaact gtacattctg taatatgagc tgtcttaaga ttctaggata tgaaaaacag
     1681 gaagacctta ttggaaagaa tgttcacaaa attattcacc attcatatat tgatggaaca
     1741 gaatatctga ctgaagattg cttgattcta aaatcaatta gaaatggtaa agggtatgaa
     1801 aatagtgaag aagttttttg gaaagctgat aatacatatt tcaatgttga ttatagtgcg
     1861 tatccacaag tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ncataatgta
     1981 ggtgacgagc ttattaagaa atctgcagaa gtcttaaaaa aagcctgcag aaaagaagac
     2041 ataatatcaa gagtaggtgg agatgagttt ataattctac ttcctaaaac agaaaaggac
     2101 atagcagaac agttgaccag gttaataaaa gctgagatgt caaactctaa agttgcagca
     2161 attagatgta gtatggcctt aggtgttaac acaaaaacac aagaaagaca ggttttggat
     2221 gaaataatag cagatgctga aaatgcaatg tataaagaca agacaatgaa tcgtcaatcg
     2281 acaaacagag acttaattaa tactattatt gaaacattgc attctagaag tcctcaagaa
     2341 aagcagcatt cagataatgt tcaaaaatta tgctcgatga tgggaaaagc tttgaagctt
     2401 tcaaaaccgg aaatagataa acttgaaaga tcggcatatc ttcatgatat aggtaaaatt
     2461 actatagata aagaaatact ctcaaaaaaa gagctaagtg aagaagagaa ggaaaaaatg
     2521 tatcagcatc ctgttgtcgg atacagaatt ttaaatttgt ttgatgaaac gcttgattta
     2581 gccgagtata tatataacca tcatgaaaga tgggatggct tagggtatcc aagaggagta
     2641 gcaaaagatg aaattccttt tatttcaaga ataatcgctg ttgtggaaac ctatgacaga
     2701 attgtatatg gatatagcgc tattcgggac aaagactcta agataaataa agaaagagca
     2761 atcagtatta ttgaagaagg ttcaggtaag cagtttgacc ctaagatagt aaaagtattt
     2821 ttggaaataa tgctaaagta aactatgtga atatgaatat atagtttgag ggaatgaaaa
     2881 gaggctatga gcatgaaaga tttatttatt caatcaggtg aagtaagacc aggagaccat
     2941 cttgttgctt tgtacaaaga tgaaaaagaa attttcgatt atgtaactgc atatatacat
     3001 tcagctctgg ctagaaatga aagttgcata tatataactg gggatgtaga tacatcaatg
     3061 gttcttaatg agattaggaa tatgacagaa gtatctcatg caaaaggtga tcttctgata
     3121 cttgatagaa ccgaaactta ttcaaaagaa ggaaaattca gccctgacag actaataaat
     3181 atgattaaag atatggtcaa gaccgctctt gatgatggat atactgctct tgctataact
     3241 ggagaaatca gctgggtatt ggattatgaa gatggggaac agctaataat tgaatatgaa
     3301 tggaaactta atgaatatat cttcaataca tatcctgtat cagcattatg caggtataac
     3361 ataaatagat tttcttatga aatggtgaag aatataattc agcttcatcc tatattaatc
     3421 tggaataata aaatacatga aaacccatac tatatacctg ctgaaggttt caaaaataat
     3481 gagatagcca agtaccaggt ggatgtatgg cttaaaaata tatttaattt tacagcaacc
     3541 aaaaaacgat ttatgacagt tttagaaaaa agtcagcaag aaatgcagaa attacataaa
     3601 aatatgacta acggtattat aatggctttt ctgaacttgt tagaaaatca tgatacatat
     3661 acaaaggatc acagtactaa tgtcgcttta ttatcatcaa agattgctga tagtttaaat
     3721 gaatctgaag agtttaaaac aaagatatat tatgtttcat tagttcacga tttgggaaaa
     3781 gcccttatac caaaggacat acttaataag cctggaaaac tcagtgatga agaatacggg
     3841 tatattaaac ttcacccaat atatggagca aatgctttaa agcagatgga tcagcttgga
     3901 gatatctccc ttgcagtgag gcatcatcat gaacgttatg acggaaaggg ataccctgat
     3961 ggtatttctg gggataatat tcctcttatg tcaagaatta ttgcattatg tgacagttat
     4021 gacgctatga taaatgacag accatatagg aaagcaaaca gtcatgagta tgctattaat
     4081 gaaataattg catgttctgg aactcaattc gatcctgtac tcgtagacca tttcgttaag
     4141 ctgttttcgt aattaaaaca atataattat acctaatgag gtataaaata tataaatata
     4201 agcaatatat taccctatcg ggtgtaatat gcagttatga atactattgg taagtacata
     4261 aaacagaaaa gaaaagaagc gggactgaca caggaggaat ttgcaatacg ttccggactg
     4321 ggtcttaggt ttgttcgaga actagaacag ggaaaagaaa ctgttaggat ggataaaata
     4381 aatcaggccc ttagtatgtt cggtatggaa acagttcctg gaaagaggga tgaataatgc
     4441 atgatcctta tagaactgcg tatatctata tacaagatat atatgctggt atattgaaag
     4501 aaactgatac tgggtactca tttcaatatg ataaagatta tttaagcaat ccggaagctt
     4561 cttctgccag tttgaccttg ccaatacagg agaaagcttt tttttcgaag actcttttct
     4621 ctttttttga tggcttgata cctgaaggat ggcttttgga tattgttact cacaattgga
     4681 aaattaacat taatgaccga tttggtatac ttttaattgc ttgtaaagat tgtataggta
     4741 acgtaagtat aagggagata aaggaatgaa ctgcttatgt tgtggtaagc ctttatctga
     4801 tgactcagct gttgagaaag cttcattctg gcataattct tgtattaaga aattcttttt
     4861 tgcaaataga ctgccaatta tagatgtttc tgagcaaatg cttatgcatc ttgcacttga
     4921 aaacacaaaa aagggattca ctatacctgg cgtacaaaaa aaaatatcgc ttcatctgac
     4981 acagggtaat aatccgagat taacgcttgt aaactatccg acaggatata ttctgaaacc
     5041 tcaggtgtct gagtatcatt ctttacctga agcagaattt ttggtcatgc agatggcatt
     5101 aaaaacaggt attaatacag ttccatttgc actattgatg atggaacata ataattattc
     5161 atatattaca agacgtatcg accgtatttt accaactgga aaaaatatta agactcaaat
     5221 gcttgccatg gaagacttct gtcagttaag cggtcgatta actgtggata aataccatgg
     5281 ctcatacgaa caatgcgcaa agataatatc gaaatattcc ataaataccg gacttgatct
     5341 gtctgagctt tttgtcagaa tagtattttc atatgtggta ggcaattcag atatgcattt
     5401 aaagtacttt tcattaattg aaactgcacc aaatagtcaa gaatatgttc tttcacctgc
     5461 atatgacatg cttcctgtaa atattattct acctgacgat aaagaccagc ttgcgcttac
     5521 ccttaatggt aaaaaacgga acataagaaa gaatgacttt cttttctttg ctgaaaattc
     5581 aagaatacct caaaaatctg cagagaagat gataaagaaa gtagtttcat tgaaagagac
     5641 atatcttaaa atgtgtcggg attcatacct gcctgatgat atgaagtcag tatttgagaa
     5701 gcttataacc gacaggatat cagttttaac tgaataaaat gcatttgttt attataagta
     5761 acatctatac acattatatt ttatcgaaag tattatataa atattatata gacagtttaa
     5821 tgaaattaag tatttggttt ttattgattt gaatatataa tatgttcaaa aggaaaaatc
     5881 caataaccat caccgatggt ttgggttata ggtgcgtgtt tatgaattcc agagaaaaag
     5941 tgaaaaatat ttttttgcgt aagagtaatg gtgcaggggc tatgtggaca ggtcatccta
     6001 atgacaagac aattccaatt tatgcaaaaa aatggaatat agaaccttca agggaagcga
     6061 tattcactta tcttgatgat gactgcagat gggtcatagc taattcagga tataagcatc
     6121 cacaagggct tgctgatttc aacccggcat ggaacaccaa gagagataca ttgagtgcag
     6181 aaggctgttt tgctgatgcg gaaaatattt ctgatataga gaaatatcca tggccggatg
     6241 ttaagtactg tgattttact gatatatatg cacagattga taaatttccg gataagatga
     6301 tatttacagg gatatggagt cattttttcc atttagttgc agactttttc ggtatggaga
     6361 attatttcat aaagatgtat gaaaacccaa agatagtaga agctgttaca gaaagaatag
     6421 tagactactg tgtggaagct aatgacctgt tctttaaagg attaggcgac agggcagatg
     6481 caatgttttt tggtaatgat ttcggcacac aacaggattt attcttgtca cctgaaatgt
     6541 tcaggaaatt cgtaatgcct tcatttaaaa ggctgataca gataggtaag aagtataaca
     6601 aaataataat actgcattcg tgcggatcga tatatagaat tattcctgat cttattgaag
     6661 caggtgttga tgtattgcat cctatacagg cgcaagctgt tggtatgagt gcaaaagatc
     6721 tttccatata caagaatgat cttgcattct taggaggcat agatgcgcag actttctttg
     6781 ttaatgccac accttctcag attaaagatg aggtaagaag agtgaaatcg attctaggtc
     6841 cgaacatcgt tatttctcca agccatgagg agattcttcc caatgtgcca gcagagaaca
     6901 tattggctat ggcagaggct gcgagggaat agatatacgt tcaaaaagta tagagatttc
     6961 aggcagggta tatcctgcct ctactttttt tatactccag ctatcatcca gattaagcca
     7021 ggattgaacc ttttctgatg aaaatgggat aaaaggtttc aaaagaactg ctgcgttagc
     7081 tattatctga gtgcagttgt aaatagcatt atcacattcc ttttgattat tcagccgagt
     7141 tttccagggt tctaatgtgt cataatactt atttccaaat cttattaatg agaatatttc
     7201 ttcaagagct tctttaagtc tgccttcttc aattttgcca gaaacaccta taaacgtgtt
     7261 ttctatcaat ttgttaatcg caatatcgga aactccttta ggaacgatgc tattttgata
     7321 tttttttgta aataaaagcg ttctgttcac aaaattacca taagttccaa gtaattcaga
     7381 gttattcctt tccgtaaatt cgcgccatgt gaaatcagta tctctttttt caggtccatt
     7441 agcagttaag aagtatctta tggaatcagg gttatatttt tctacaatgt cttttgccca
     7501 tatagcccag tttttacttg tggatatttt tcttccttca agtgtaagat gttcattaga
     7561 tattatgtca tctggcagat gagtatactg attatgagca agcagcagtg agggaagtat
     7621 aatcgtgtgg aaaggtatgt tatctttacc atgaacataa tagtgacgcg aattatcttt
     7681 ccaaagagca ctaaaaggta tatctttttt ttcacatagc actttacttg cagaaagata
     7741 ccctaataca ttttctgccc atatgtatat ccttttcttt tcatatcctt gttttggtac
     7801 ttctataccc cattccagat cacgtgttat tgcacggtct ttaagtcctt cgtcaatata
     7861 tctcttagta aaagcaacag cgttttttcg ccagttgtta tgcttatcaa gatattttct
     7921 taattgattt tccagttttg atatggctat atacagatgt tttgtgttat agaagaatac
     7981 aggattttta cattcagaac atattgggtt ctttatactg gttacatcat ttacagttgt
     8041 acagaaatcg cactgctcac cattggtttc atttccgcat atcggacatg ttccaattac
     8101 cagcctgtcc gttaatggtt tatcgcaatt attacagtat gccgaaggtg aatccagttc
     8161 atatacatat ttacttttat agagctcttt atggaaattc tgaacaaaat ctatatgcca
     8221 tttatctgtc gtttttccat ataggtcata actgaatccc aatctgttaa aaacttctgt
     8281 aaattcatta tgatatttat cacagatttg ttggggtgaa gtattttctt tttttgcttt
     8341 tatgcagatt ggcgtaccat gacagtcagt gcctgagaca taaaaaacat tatctttttt
     8401 tgctctgtaa taacgcgcta ttatatcacc aggtaaaaga cctgctatat gtccaatgtg
     8461 taatgagcca tttgcataag gccatgctcc tccgattaat atgttcattt cattttttac
     8521 atgttgcagt catctgtcgc aacatttttc tcctttcaaa atcaaaatat cttatatcta
     8581 attaaaaaaa aggtgtaagc tttgtggctt acaccttgca tatccttctt ttttttattt
     8641 actcagggta tgatgatact ggccacaggt cgtgaagctt tacattcatc atttgcatta
     8701 ttgtgtgttt agttttaatt tcactaatat tacagttcat catcattttc cttccggtta
     8761 ttcttaaata ttatgattag tctagtacta ttgtttgctg atgtcaacta tttagattat
     8821 tt
//
LOCUS       DAOSXM010000086         8500 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2111
            NODE_5315_length_8500_cov_10.171226, whole genome shotgun sequence.
ACCESSION   DAOSXM010000086 DAOSXM010000000
VERSION     DAOSXM010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864178
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 8500)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8500)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:24:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,261
            CDSs (total)                      :: 2,229
            Genes (coding)                    :: 2,218
            CDSs (with protein)               :: 2,218
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 3 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8500
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5315_length_8500_cov_10.171226"
                     /isolate="AMR_MDS_2111"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..396
                     /locus_tag="PLQ68_09220"
     CDS             <1..396
                     /locus_tag="PLQ68_09220"
                     /inference="COORDINATES: protein motif:HMM:NF027666.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4976 domain-containing protein"
                     /protein_id="HXK72873.1"
                     /translation="IPATICHLCDIPMNPKAHGRSLVDMLSKNSFDLDKFPNYTVSCS
                     NGQQFGSYTQRSIRTKHFKYIWNLTDIDEFYDLKKDPYELENKVYEEKYKDTISDLRS
                     ELKKELTRCEDPFVKSGWLNRTLDENVKL"
     gene            complement(408..725)
                     /locus_tag="PLQ68_09225"
     CDS             complement(408..725)
                     /locus_tag="PLQ68_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015711887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HXK72874.1"
                     /translation="MERYAWRAKVIEGKLDEYIKRHNEIWPELADLLKKAGIRNYTIW
                     YNNNDLFGYYECEKGVEYAARIQAQSPIVDRWNEYMKDVMVMEMDPVTKAQPLMEQVF
                     LLE"
     gene            complement(743..1657)
                     /locus_tag="PLQ68_09230"
     CDS             complement(743..1657)
                     /locus_tag="PLQ68_09230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009831989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HXK72875.1"
                     /translation="MFVSKYRLITRSDFDGLVCAMMLKELDMLDSILFVHPKDMQDGM
                     VDVSDKDITTNVPYQQGVHLAFDHHISEKERRYVNYINIPDAASTASVVYEYFGGEKK
                     LKISKEIISAVDKSDSAQFTYDDIVNPKDWVLLSFIMDARTGLGRFKDFRISNYDLMM
                     MLIDYCSCHTVEQVLEHPDVKERTDMYRKQQELFKNQLLECAEVINDVVIIHLKNQDP
                     IYVGNRFLIYTLFPKATVSIHEMWGLKKRNTVFTLGKSIINRGSDKDLAFICKKHGGG
                     GHMNAATCQVPNGLADEVLKGILNDLGV"
     gene            complement(1824..3416)
                     /locus_tag="PLQ68_09235"
     CDS             complement(1824..3416)
                     /locus_tag="PLQ68_09235"
                     /inference="COORDINATES: protein motif:HMM:NF025067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HXK72876.1"
                     /translation="MKIFKFILNSVKEAMSKFVVSFYGSIIIFILASIQVIFDVDEEI
                     YMRIIFAVAFSVLASIMFQLMSLQYNYSKKMDIIQKIFSSALAIPCYFIIKNIFESDY
                     IFMGYFGLILCFIVISLFLMYNCEKQDLIFPQIIKSGFFSVIVASLFFGGIALCLLAF
                     DFLVTELPDFDNWFIITGLLAYIVICFNLFIALLPEKKDNINLPKIFKVLVLYVGLPI
                     YTLLLAVLYAYLVKILITMEMPQGQINIFASLASLFFIFFYLNLGSYENRAVSFFRKF
                     GGWFLLPIIASQAVAIYIRVNAYGLTTARWISILLNVTALAFIIISLIKQGKFVKHVI
                     TGFAVLILISSIGPLNAMDIPIYEQSHRLEKVLETNGMFYDGKISPKSDLTKEDMRII
                     SSCYDYLIYTDNPPVYLDKDLTFYSLFGFEQLNSYGEPDPYNMIHLNKYVQYDDIDIS
                     EYSRYIKVAAYSNDKFEMTIEDIAYDIADEIMAIKQDDNKEDMIFSLSSDITVYLTTV
                     YYEMDNENNIMYFSAEGFALIK"
     gene            complement(3449..5341)
                     /locus_tag="PLQ68_09240"
     CDS             complement(3449..5341)
                     /locus_tag="PLQ68_09240"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HXK72877.1"
                     /translation="MNNIKYDFKRRAKELVSKMTLAEKTAQMQYESEAIDRLSIPAYN
                     WWNEALHGAARSGTATVFPQSIACAASFNDKLMKKVGNAISDEVRAKYNQYSKFNGTE
                     IYQGLTCWSPNINIFRDPRWGRGHETYGEDPLLTAKMGTAYIKGMQGNDKAYKKVDCT
                     LKHYAVHSGPESLRHSFDAFVSEKDLYETYLFAFKYCIKHSNPSAVMGAYNRVYGEPC
                     CGSSYLLEKILRKEFGFKGYVVSDCGAICDFHKYHKVTENEAESAALAVKSGCDLNCG
                     DTYKWLKVAVAEGMLTEEDITKSSERLFEARFRLGMFDKECRYNQIPYSVICSKKHKK
                     LNLDMARESIVLLKNSGILPLSNDKKIAIIGPNADDLSVLLANYNGTADDYYTPLRGI
                     RKLCKNGVIYARGCHVTEDESTWRYGEHPMREAIIAAKHSDVVIMCMGINPQIEGEEA
                     LHKGFKGDRDSIEMPLIQQKLFKEIRKTNKPIIFVNISGSCIDLSLPDKEADAVIQCF
                     YPGALGGIALADIIFGRVSPSGRLPVTFYKDTTDLPDFEDYSMENRTYRFFKGKPVYS
                     FGFGLSYADVEEKWIDENTVEISNKSDIDTDYSVLKFQKKPDMKLLDFKKVKLKGNSK
                     KIVIFS"
     gene            complement(5348..>5830)
                     /locus_tag="PLQ68_09245"
     CDS             complement(5348..>5830)
                     /locus_tag="PLQ68_09245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HXK72878.1"
                     /translation="AWIFTWHYGALALSLVLMLIILLGLIFINNLLAKADLTLKDKFL
                     LKLPFSIYYGWITVATIANVTAFLVDLGWNRWGFREVFWAIIAIGAGLVIGGLTALVN
                     RDVPYALVLIWAYAGIVIKHVSVTGFNNQYPQVIIACSVAITLFVVDLIYIVIKNRKV
                     "
     assembly_gap    5831..5930
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            6066..>8500
                     /locus_tag="PLQ68_09250"
     CDS             6066..>8500
                     /locus_tag="PLQ68_09250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006567538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HXK72879.1"
                     /translation="MAKKVVIIGGVAGGASAATRLRRLDEQAQITIIERGEYISYANC
                     GLPYHIGNVIKQRSSLLLLTPEVMKQKYNIDVRIKQTVTSIDRKNKTITVKRADNSEY
                     SLEYDKLVISTGSSPLMPPIPGIESSRIMTLWSVPDTDKIKAYVSDSQVKSVTVVGGG
                     FIGLEMAENLKGIGLKVTVVEMLNQVMTPFDFEMAQLIHEELEQSGIRLILNNGVASF
                     SDKGSGVQICLKDGEELYSDLVLLSIGVRPNSDLGKASGLSLNAKGGLVTDEHMKTED
                     DDIYAVGDVIEVEDFIDKTRTMIPLAGPANKQGRIAADNIAGIDSVYHGTQGTSIVRV
                     FGLTAANTGYNEKYMLRKGFVKGKDYETLIITQNSHAGYYPGALPLTLKLIFSKDGSK
                     IYGAQIIGRDGVDKRIDTIATAMRLNASVFDLKNLEFAYAPPFSSAKDPVNMAGFVAE
                     NIIKDLVEFSEWDVIEKNQDILILDIREDAERMAFSLPNTLNIPLGQLRDRLDEIDKD
                     KHILIFCTIGVRSYNALRLLKEKGFSKVTVYPAGTRFYISTHYKEFHMEDHTSYFVSD
                     SGRQGDDKAVASISLDCSGLQCPGPIMKVFETLNTMAPGQTLEVTASDPGFSRDISAW
                     CRRTGNTLVSNNKVGTSYVAVVRKGGGSVPVPEQVGNDGKTIIVFDGDLDKVIASFII
                     ANGAAAMGRRVTMFFTFWGLNVLRKTQKQNVKKSFIEKMFGAMMPRGISKLKLSKMNM
                     GGMGTAMMKKIMNDKNVDSLEELVRKAMKAGVKIVACTMSMDVMGIKEEELIEGVELG
                     GVGAYLADAEESNV"
BASE COUNT         2799 a         1532 c         1376 g         2693 t
ORIGIN      
        1 atacctgcca caatctgcca tctttgcgat ataccaatga atccaaaagc ccatggcaga
       61 tcgcttgttg atatgttaag taaaaactca ttcgatttgg ataaatttcc taactatacg
      121 gtatcatgct caaacggaca gcagtttgga agttacactc aaagaagcat acgaacaaaa
      181 catttcaaat atatatggaa tctgactgat attgatgagt tctatgacct taagaaagat
      241 ccatatgaac ttgaaaataa agtttatgaa gagaaataca aagatacaat ttctgactta
      301 agaagcgaat taaaaaagga acttacaagg tgtgaagatc cttttgtaaa aagcggatgg
      361 cttaatcgaa cacttgatga aaatgtgaag ctgtagaaat acagctttca ttctaataaa
      421 aatacctgtt ccattagagg ttgagctttt gtaacaggat ccatttccat aaccattaca
      481 tctttcatat attcattcca tctgtcaacg ataggacttt gcgcttgaat tctggctgca
      541 tattcaacac ctttttcaca ttcataataa ccaaaaagat cattgttgtt ataccatata
      601 gtataatttc ttatgcctgc tttcttaaga agatctgcta attcaggcca aatctcatta
      661 tgtcttttaa tgtactcatc taatttgcct tcaataactt tagctctcca tgcatatctt
      721 tccatcttat ccaattatcc tcctacactc caagatcatt taaaattcct tttaaaacct
      781 catcagccag gccatttgga acctggcagg tagctgcatt catatggcct ccgcctccat
      841 gtttcttaca aatgaaagca agatctttat cagaacctct atttataatt gatttgccga
      901 gagtgaatac agtgtttctt ttcttcaaac cccacatttc atgtatggat acagttgctt
      961 ttgggaaaag agtataaatt aaaaatctgt ttccaacata aataggatcc tgattcttta
     1021 aatgtattat tacaacatca tttataactt cagcacactc taaaagctga tttttaaata
     1081 attcctgctg ctttctatac atatctgttc tttcttttac atcaggatgt tcaagtacct
     1141 gctcaacagt atgacaagaa cagtaatcta taagcatcat cattagatca taatttgata
     1201 ttctgaaatc tttgaatctt ccaagaccag tccttgcatc catgataaaa cttaaaagca
     1261 cccagtcctt cgggtttact atatcgtcat atgtaaattg tgctgaatca gacttatcaa
     1321 ctgcactgat tatctctttg gatattttca gttttttttc tccaccgaaa tattcgtata
     1381 cgactgatgc agtagaagcc gcatcgggta tgtttatgta attaacatat cttctttctt
     1441 tttccgagat atgatgatca aaggctaagt gaactccctg ctgataagga acatttgttg
     1501 taatatcctt atctgataca tccaccattc catcctgcat atcttttgga tgcacaaata
     1561 gaatactatc cagcatatca agttccttta acatcatggc acaaaccaat ccgtcaaaat
     1621 ccgatcttgt tattaacctg tatttgctca caaacaacct ccgaattaaa atgcatcaat
     1681 accaatattt tactgtttga caaaggattt ttcaacaata attacttata tgttaacaca
     1741 attatttata attaaagtat atgatttaaa aattttaaaa atacatttaa tttcaataaa
     1801 tttgtcatat taaattacaa tatttattta attaatgcaa agccttcagc agagaaatac
     1861 ataatgttat tctcattatc catttcataa tacactgttg tgagataaac agttatatct
     1921 gaagaaagac taaatatcat atcttcctta ttatcatcct gctttatggc cataatctca
     1981 tctgcaatat cataagctat atcttcaatc gtcatttcaa atttatcgtt actatatgct
     2041 gcaaccttta tataccttga atattctgaa atatcaatat catcatattg tacatattta
     2101 ttaaggtgta tcatattata tgggtcaggt tccccataac tgtttagctg ttcaaatccg
     2161 aataaagaat aaaatgttaa atctttatca agataaacag gcggattatc tgtatatatg
     2221 aggtaatcat aacagctgct gataattctc atatcttctt ttgttaagtc tgacttaggt
     2281 gagatttttc catcataaaa catgccattt gtttccagaa ccttctcaag cctgtgagac
     2341 tgttcatata tcggaatatc catagcattt aaaggcccta tggaagatat aaggataaga
     2401 acagcaaacc cggtaattac gtgctttaca aattttcctt gtttaattaa agatatgatt
     2461 atgaacgcaa gagctgtaac attgagcaat attgatatcc atcttgctgt tgtaagccca
     2521 taagcattaa ctcttatgta aatagctact gcctgtgatg cgattattgg aagcagaaac
     2581 cagcctccaa acttcctaaa aaagcttact gccctgtttt cgtaacttcc aagattgaga
     2641 taaaagaaaa tgaagaaaag tgatgctaaa gatgcgaata tatttatctg tccctgaggc
     2701 atttccattg ttatcaatat tttaaccaga tatgcgtata acacagcaag caaaagagta
     2761 taaataggaa gacctacata aagaacaagt accttaaata ttttgggtag atttatatta
     2821 tctttctttt caggcagaag ggctataaaa agattaaaac aaatgacaat ataagctaga
     2881 agacctgtaa taatgaacca gttgtcaaaa tcaggaagct cagtcactaa aaagtcaaat
     2941 gctaaaagac aaagagctat tcctccaaag aataaagaag caactataac agaaaagaat
     3001 cccgatttta ttatctgtgg gaaaataagg tcttgcttct cgcaattgta cataagaaac
     3061 agagatatga ctataaaaca cagaatcaat ccaaaataac ccatgaatat ataatcagac
     3121 tcaaatatat ttttaattat aaaatagcat ggaatggcaa gagcagaaga aaaaatcttt
     3181 tgaataatat ccatcttttt gctgtagtta tactgaagac tcataagctg aaacattatt
     3241 gaagctaaaa cggaaaaagc aacagcaaat ataatcctca tatatatctc ttcatcaaca
     3301 tcaaatatta cctgtataga agctagtatg aatataatta tacttccata aaatgatacg
     3361 acgaactttg acatcgcctc cttaacactg ttaagaataa atttaaaaat tttcattgcc
     3421 ccctccaaaa attatcatat aaaacttgtt aactaaatat tactattttt ttactatttc
     3481 cttttaattt gactttttta aaatctaaaa gtttcatgtc aggtttcttc tgaaatttaa
     3541 gaacagaata atctgtatct atatcgcttt tattagagat ctcgacagta ttttcatcta
     3601 tccatttttc ttctacatca gcatatgaaa gaccaaatcc gaaagaatat actggttttc
     3661 ccttaaaaaa tctgtatgtt cgattttcca ttgaataatc ttcaaaatca ggaagatccg
     3721 ttgtgtcctt gtaaaaagtc acaggcagcc tcccgcttgg acttactcta ccaaaaatga
     3781 tatctgctaa cgcaataccg cctaaagcac ctggataaaa acactgaata acagcatctg
     3841 cttctttgtc aggcaatgaa agatcaatac agcttcctga aatattgaca aagattattg
     3901 gcttattcgt ttttcttatt tccttaaata atttttgctg tattaaaggc atttcaattg
     3961 agtctctgtc tcctttaaac cccttatgta atgcctcttc tccttcaatt tgcggattaa
     4021 ttcccatgca cattataaca acatcactat gctttgccgc tataattgcc tctctcatag
     4081 gatgttctcc atatctccag gtagattcat cttctgttac atgacatccc cttgcataaa
     4141 ttactccgtt tttacaaagt tttctaattc ctcttaaagg agtgtagtag tcatctgctg
     4201 ttccattgta atttgccaag agaacagata aatcatctgc attgggacca ataatggcaa
     4261 ttttcttatc gttacttaac ggaagtattc ctgagttctt gagcagaact atactttctc
     4321 tagccatatc aagattcagt tttttgtgct ttttactgca tattacactg tagggtatct
     4381 gattatatct acattcctta tcaaacattc ctaatcggaa tcttgcttcg aataatcttt
     4441 ctgatgattt agtaatgtct tcttctgtaa gcattccttc tgcgacagct acttttaacc
     4501 atttatatgt gtctccacag ttaagatcgc aaccgctttt tactgccaaa gcagcacttt
     4561 cagcctcatt ttccgtaact ttgtgatact tatggaaatc acatatagcc ccgcagtctg
     4621 aaacaacata tcctttgaag ccaaactcct tccttaatat cttttctaga agataagaac
     4681 ttccacagca tggctcacca taaactctat tatatgctcc cattacagca gatgggtttg
     4741 agtgttttat acagtattta aatgcgaata ggtatgtctc gtacagatct ttttcactta
     4801 caaatgcatc aaaactgtga cgtagtgatt ccggtccgct gtgcactgca taatgtttta
     4861 atgtacagtc gactttttta tatgccttat cattaccctg cattcctttt atatatgctg
     4921 tacccatttt cgctgtaagc agtggatctt ccccatatgt ctcatgtcct cttccccatc
     4981 tcggatcacg gaatatattt atattaggtg accagcaagt aagaccctgg taaatttcag
     5041 taccattgaa tttactgtat tggttgtatt tggctctaac ttcatcagat attgcattac
     5101 cgaccttttt catgagttta tcattgaatg aagctgcaca ggcaatagat tgtggaaaaa
     5161 ctgttgcagt cccgcttctt gctgctccat gcaatgcttc attccaccag ttatatgctg
     5221 gaatggataa tctgtcaatt gcttcagact catattgcat ttgtgcagtt ttttccgcta
     5281 aagtcatctt acttacaagt tctttagctc ttcttttgaa atcgtatttg atattattca
     5341 taactattta aactttccta ttttttataa ctatatatat taagtctaca acaaacagtg
     5401 taatcgcaac tgaacatgcg ataataactt gaggatactg attattaaat cctgttacgg
     5461 atacatgctt tattactatt cctgcatatg cccatatgag tacaagagca taaggtacat
     5521 ctctatttac taatgctgta agtccgccta ttactaaacc tgcaccaata gcaataatag
     5581 cccagaatac ttctcgaaag ccccagcgat tccatcctaa atcaactaag aatgctgtta
     5641 cattagcaat cgttgcaaca gttatccatc catagtatat ggaaaaagga agctttaaca
     5701 aaaacttgtc tttcaatgtc aaatcggctt tagccagcag attgttaatg aatataagcc
     5761 ctagaagaat tataagcata agtacaagac ttaatgccaa agcaccataa tgccatgtga
     5821 atatccatgc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn tgtatacttt
     5941 ttttgtaatc gaatatcaat tcatgtattt ttactgtgtt aaattattaa cggtgacaaa
     6001 gtcacttttt ttatgcaaag aagtgatatt attatattga caatatttta acaggaggca
     6061 tgctaatggc taaaaaagta gtaataatcg gaggagtggc tggaggtgcg agtgcagcga
     6121 caagacttag acgccttgat gaacaagccc agataaccat aatcgaaaga ggtgaataca
     6181 tatcctatgc caattgcggg cttccttatc acattggtaa tgtaattaag caaagaagtt
     6241 ccttactgct gcttacccct gaagtgatga agcagaaata taatattgat gtaagaatta
     6301 agcagacagt tacttcaata gacaggaaaa ataagactat aacagtaaaa agggcagata
     6361 attctgaata ttctctggaa tatgacaaat tagtaatatc tacaggatca tcacctttaa
     6421 tgccacctat cccaggcata gaatcatcta gaattatgac tttatggtct gttcctgata
     6481 ctgacaaaat caaagcatat gtttctgaca gccaggtaaa atccgtaacc gtagttggag
     6541 gaggttttat cggattggaa atggcagaaa atctaaaggg tatcggctta aaagtaacag
     6601 tagtggaaat gctcaatcag gtaatgactc catttgattt tgaaatggct caactgattc
     6661 atgaggaatt agagcaaagc ggaattcgct taatactgaa caatggtgtt gcttcttttt
     6721 ctgacaaagg aagcggtgtt cagatttgcc ttaaagatgg cgaagaactt tattctgatc
     6781 ttgttcttct ttcaataggt gttcgtccaa acagtgattt aggaaaggct tcaggtttat
     6841 cattaaatgc taaaggcgga ttggtaactg atgagcacat gaaaaccgaa gatgatgata
     6901 tatatgcagt tggtgatgtt attgaagttg aagattttat tgataagacc cgaacaatga
     6961 taccgcttgc agggccagca aataaacaag gaagaattgc tgcagataat attgcaggta
     7021 ttgattctgt atatcacggt actcagggaa catcaattgt aagagtcttt ggacttacag
     7081 ctgcaaatac aggatataac gagaaatata tgttaagaaa aggttttgtt aaaggaaagg
     7141 attatgagac tttaatcata acccagaact ctcatgcagg atattatcca ggagctttac
     7201 cactgacact aaaattgata ttctctaaag acggatctaa aatttacggt gcacagatta
     7261 tcggccgtga tggagtagat aagagaattg atactattgc aacagcaatg aggctcaatg
     7321 caagtgtatt tgatttgaaa aaccttgaat ttgcatatgc tcctcccttc tcctctgcta
     7381 aagatcctgt aaacatggca ggattcgtag ctgagaatat tataaaagac cttgtggaat
     7441 tttctgaatg ggatgtaatt gaaaaaaacc aggatattct gattttagat ataagagaag
     7501 atgctgaaag aatggctttc tccctgccta atacactgaa tataccatta ggccagttaa
     7561 gagacaggct ggatgaaatt gataaagaca aacatatact tattttctgc acaataggtg
     7621 tcagatcata taatgcactc agactgttaa aagaaaaagg attcagcaaa gttaccgtat
     7681 atcctgctgg aacacgtttt tacatatcaa cacactataa ggagtttcat atggaagatc
     7741 atacatcata ttttgtatca gacagcggtc gtcagggtga cgacaaagca gttgcttcaa
     7801 taagcctcga ttgttccggc cttcaatgcc ccggacctat aatgaaagtt tttgagacac
     7861 tcaacacaat ggcacctgga caaactttag aagtaacagc atcagaccct ggtttttcaa
     7921 gagatatatc agcatggtgc cgccgtacag gcaatacact agtatcaaat aataaagttg
     7981 gcacaagtta tgttgctgta gtaagaaagg gtggtggttc tgtccctgtt cctgaacagg
     8041 taggcaatga cggaaagacc attattgtat ttgacggtga cctagataaa gtaattgcta
     8101 gttttatcat tgctaatgga gctgctgcaa tgggaagaag agtcacaatg ttctttactt
     8161 tctggggatt gaatgtatta agaaagacac aaaagcagaa tgttaagaaa tcattcattg
     8221 aaaaaatgtt cggagcaatg atgccaagag gaattagtaa gttaaaatta tcaaaaatga
     8281 acatgggcgg aatgggaaca gccatgatga aaaagataat gaatgacaaa aatgttgatt
     8341 ctctagaaga acttgttaga aaagctatga aagctggagt aaagattgta gcctgcacta
     8401 tgagcatgga tgtaatggga atcaaggaag aggaactgat tgaaggtgtt gaacttggag
     8461 gagttggagc atatttagct gatgctgaag aatccaatgt
//
LOCUS       DAOSXM010000087         8374 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2111
            NODE_5463_length_8374_cov_10.273230, whole genome shotgun sequence.
ACCESSION   DAOSXM010000087 DAOSXM010000000
VERSION     DAOSXM010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864178
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 8374)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8374)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:24:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,261
            CDSs (total)                      :: 2,229
            Genes (coding)                    :: 2,218
            CDSs (with protein)               :: 2,218
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 3 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8374
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5463_length_8374_cov_10.273230"
                     /isolate="AMR_MDS_2111"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(171..1889)
                     /locus_tag="PLQ68_09255"
     CDS             complement(171..1889)
                     /locus_tag="PLQ68_09255"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HXK72880.1"
                     /translation="MAIGGKGLYEVRQVKDIREAIFGAADIYGDKDALLEKNKVTDKY
                     EAYSYNRFIKDIKSLGTALCNMGLKDKRVVVMGENRYLWSVSYMAVVNGTGVVVPVDV
                     QLSQDDLKFIFESSKPEAVIYTDKKAKEIKNLSEQLPSIKYFINMDGTDTDNERFFNM
                     NSLIEKGEKLITDGDRTFIDAKIDPEEILIILYTSATTSVSKAVQLSHRNIVANLMAM
                     SSMINVRSDDVFLSILPLHHTYECTCGFICPLYLGASIGYAEGLKHIKKNMDELKPSV
                     MLSVPLLFEGMYKNIIKTAEKAGSLKKLTTIIKVADALHLSRNTRRKLFSAVLAGLGG
                     NMRLMISGAAAISPTVAKFYRSIGVAFLQGYGLTECSPIICLNSDKEFIDASAGKPLP
                     GVEVVILDKDTDGIGEICAKGPNIMKGYLDMPEENERIFEGGYFHTGDLGYMDDKDFV
                     FITGRKKSMIVTKTGKNIFPEELEQKLAKSKYVEECLVFGVNDKEGDSIVTAAVFPAL
                     QVFEDEFKNTDKKFMYNKIMELVKEVNAQVPTYKAIKKLVIRDTEFVKTTTRKIKRYG
                     ENLKVD"
     gene            complement(2047..3312)
                     /gene="ftsZ"
                     /locus_tag="PLQ68_09260"
     CDS             complement(2047..3312)
                     /gene="ftsZ"
                     /locus_tag="PLQ68_09260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012959479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HXK72881.1"
                     /translation="MLQFEMEMEQFAQIKVIGVGGAGNNAIDRMIVSGLKGVEFISIN
                     TDKQALMNSKATTKIQIGEKLTKGLGAGSIPEIGERSAEESKDIIIENIKGADMIFIT
                     AGMGGGTGTGAVPIIAQEARQMGILTVCIVTKPFTFEGRKRKENAEKGLARLKSVVDT
                     LIIVPNDRLLQISDKKITIMEAFNTADNVLFKGVQSISDLIAVPGLINLDFADVKTVM
                     TDAGIAHMGIGRGTGEEKCEEAAMEAIKSPLLETSIEGAKKILVNITGGPDMGLFEVD
                     HAANKIYEAADESANIIWGSTIDPNMTDQIMITIIATGFDDDVDESEPSVLLQDSVKK
                     PEHQTVALKEKEQEQKSHQEQTKVNAEDEDSEKIEDIKSTRIFEINLPQEEDEEAEKT
                     EENEVLNKEEPIKKKEDDSITIPSFLRTK"
     gene            complement(3424..4044)
                     /locus_tag="PLQ68_09265"
     CDS             complement(3424..4044)
                     /locus_tag="PLQ68_09265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72882.1"
                     /translation="MEQTKIRYGDNLNTMLIKQGNLFTGHLTKAEEILSSDIYKKQIV
                     VNRTGKNDIEISYSRRKPVICILYDEYYLYCDYDGVVLYASKLKEENSIYIEDIELNC
                     FYIGLDISTCTYMFEPVSVLCYEIEKYDKENFTAIRALISNIELKDNNRISIKYDKRI
                     EIVMDMTKDVRYSANVMCNILSQINQTVKGYIDFTISKTPYFTPES"
     gene            complement(4581..5495)
                     /locus_tag="PLQ68_09270"
     CDS             complement(4581..5495)
                     /locus_tag="PLQ68_09270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014042112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HXK72883.1"
                     /translation="MILVTGGCGFIGSHITDELINKGHDVIVADNLISGSMINLNPKA
                     KFIKADVTNIEQIEEIFSKYEIKAICHHAAQISVAVSMERPQFDVNTNVIGIINLLQM
                     CTKYSVKRFVFASSAAIYGNPEYLPIDESHPLNPLSFYGLSKQMSEQCIRIYANNSNM
                     TYGILRYANVYGPRQKAEGEGGVVAVFMNRMVSDQDCNIHGDGKQTRDFIYVKDIAKA
                     NVKALESNLNFTANVSTNVYTDISELFSAMKKQTGYTKQPIFTKPRKGDIKDSYLKNT
                     VINDLLKWEPVYNLEKGLKETAKYFMNA"
     gene            complement(5497..6903)
                     /locus_tag="PLQ68_09275"
     CDS             complement(5497..6903)
                     /locus_tag="PLQ68_09275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase/phosphomannomutase family
                     protein"
                     /protein_id="HXK72884.1"
                     /translation="MIKFGTGGWRAIIGDEFTKANIQVLVQGLCDKIHKENKQEKPVI
                     IGYDRRFLSEEGAKWSAEVFCGNSIKVHFIYKNSPTPLIMFQVKKETAYYGIAVTASH
                     NPALYNGIKLFTEGGRDAAMEITDEIEGYIKKVKEIKSMPFDKAVNEHMIEIIDPFND
                     YIDSIMVFLDKSAIINARLKVLLDPMHGVSKTSLQTILLTLRCDVDVINDRHDTLFGG
                     KLPSPSAATLGKLSAMVVEDNYDIGLGTDGDADRLGVIDDKGRFIHPNEIMMLLYYYL
                     VKYKGWKGDVVRNIATTHVLDDMAADFGFMCHEVPVGFKHISSKLDQTDAVLGGESSG
                     GLTVRGHISGKDGIFAASLLVEMMAVTKKKLSVILKEIYDKYGYRIMIENSYTFDHDK
                     KPKLSKMLYEDKLIPEFGLPIEKLSYEDGLKVYFNNGGWLVARFSGTEPLLRVFAEMP
                     DEKQAQDVCDRMKGFLGI"
     gene            complement(6896..7651)
                     /locus_tag="PLQ68_09280"
     CDS             complement(6896..7651)
                     /locus_tag="PLQ68_09280"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HXK72885.1"
                     /translation="MKAVVLAGGKGKRIGSEKEGLPKALRLVNGKPMIDYVLKELSFC
                     DEIVIVVGYKKDLVFEHVGTKYSYAVQEEQLGTGHAVKCASEFFEDYDGPVLVTFGDM
                     PLLTKKTYKKVLKAYNDNIKKNDPAACVMLSVMLDDVYLPYGRVIRDENKGYSRIVED
                     PDCDEQQKKIKEVFFGVMLFNGIKLKNILNRMTNNNAQNEYYLTDLPTLLKKEGEKVV
                     ICMINEQDEVYGVNTMEDLDRVESIMRKRGAND"
     gene            complement(7661..>8374)
                     /locus_tag="PLQ68_09285"
     CDS             complement(7661..>8374)
                     /locus_tag="PLQ68_09285"
                     /inference="COORDINATES: protein motif:HMM:NF024598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycoside hydrolase"
                     /protein_id="HXK72886.1"
                     /translation="VVDYICPMIYPSHYTTGWYGFEYPDMNPYGMVLGAMKDSIEKNS
                     AFNDNGKVRLWIQDFTAKYLYQPDSIYYYGYEQIYGQVKALRELGSFSYMFWNNGISY
                     DASKYIFPNDISGYPLADGDKDPIGRTPAMAAKEYLSVLSSTNNLNQYMLFVLTPIDY
                     RENDYDEWLENEFPVIKQTKILGYTIGEYVITNDNEKSAEVSVTLKYTKGDDVTEIYK
                     TVLWKAVFENGIWKIIPEF"
BASE COUNT         2534 a         1684 c         1068 g         3088 t
ORIGIN      
        1 aagattcgtc tctctctctt tttattcatt tccctacagt aactttactc taacgatttt
       61 tcacaaatat aattgtaatt gagaaaatac aattattgat taaaccaata ttaagaaaag
      121 aagcatgcat aacatgcttc ctcttccccc tctttcatat ggttaatatg ttaatccact
      181 ttgagatttt caccatatct tttaattttt cttgttgtag tctttacaaa ctcagtatct
      241 cttattacca gtttcttaat cgccttgtat gtcggaacct gagcattaac ttcttttaca
      301 agttccataa ttttgttata catgaacttc ttatccgtat tcttaaattc atcttcaaat
      361 acctgtaaag ctggaaatac tgctgccgta acaatactat caccttcttt atcattaact
      421 ccgaagacaa gacattcttc aacatacttg ctttttgcaa gtttttgttc aagttcttct
      481 gggaatatgt tctttcctgt ctttgtaact atcatggatt tctttcttcc tgtaataaac
      541 acgaaatcct tgtcatccat atatcccaaa tcaccagtat ggaaataacc accttcgaaa
      601 atcctttcgt tttcttctgg catgtcaaga taccctttca ttatattcgg tccttttgca
      661 catatttcgc caataccatc tgtatcctta tcaagtatga ctacctcaac tcccggtaat
      721 ggcttgcctg ctgatgcatc aataaattct ttgtcagaat ttagacagat tattggtgaa
      781 cactcagtaa gcccatatcc ttgtaaaaaa gctactccta tagatctgta gaacttcgca
      841 actgttggag aaattgctgc agctccagat atcattagcc tcatatttcc gccaagtcca
      901 gcaagtacgg ccgaaaaaag ttttcttctt gtgtttctgc taagatgaag agcatctgca
      961 actttaataa tagttgtaag ttttttaaga cttcctgctt tttctgctgt cttaataatg
     1021 tttttataca taccttcaaa cagtaaagga actgaaagca ttacacttgg ttttaactca
     1081 tccatattct tttttatgtg ttttaatcct tctgcgtacc ctatcgatgc acccaagtat
     1141 aaagggcata taaatccgca ggtgcattca tatgtatgat gaagtggcag aattgataaa
     1201 aatacatcat ctgatctaac gttaatcata cttgacatag ccattagatt tgcaactatg
     1261 tttctatggg ataactgaac tgctttagaa actgaagtag ttgctgaagt ataaagtata
     1321 attagaattt cttcaggatc aatctttgca tcaataaatg tgcggtcacc atcagttata
     1381 agtttttctc ccttttcaat taaactgttc atattgaaaa aacgttcatt atcagtatca
     1441 gtcccatcca tattaataaa atatttaata cttggaagct gttctgaaag attcttaatc
     1501 tcttttgctt tcttatctgt gtatattaca gcttcaggtt ttgaagattc aaatataaat
     1561 ttcaaatcat cctggcttaa ctgaacatca acaggaacta ctactccagt gccattaaca
     1621 acagccatgt atgacacaga ccataaatat ctgttttctc ccatgacaac aactctttta
     1681 tctttcaacc ccatattaca taaagctgta cctaagcttt ttatatcctt tataaatctg
     1741 ttataagaat atgcttcata tttatctgtt accttatttt tttctaataa tgcgtcttta
     1801 tctccataaa tgtcagctgc tccaaaaata gcttctcgta tatctttgac ctgtctgact
     1861 tcatacaacc cttttccgcc tattgccata acttattacc tccattttct tacattatat
     1921 atcagtaaaa gatgaaatgc aatacttttt atcacttggt aataaaatgt ttacaaacaa
     1981 ataaaatgcg tgttaccgca ttttaaatta actatttttt tatatttatt gcaaaaacaa
     2041 taatatttat tttgttctaa gaaaactcgg aatggtaatg ctgtcatcct cttttttctt
     2101 tatcggttct tctttattaa gaacttcatt ttcttctgtt ttttcagctt cttcatcttc
     2161 ttcttgagga agattaattt caaatattct tgttgatttt atgtcttcga ttttttcgga
     2221 atcttcatcc tcggcattta cttttgtttg ttcctggtga gatttctgtt cttgctcttt
     2281 ttctttcaaa gctacagttt gatgttcagg tttttttacg ctatcttgca ataacacaga
     2341 aggttcagat tcatcaacat catcatcaaa gcctgttgct atgatagtaa tcataatttg
     2401 atctgtcata tttgggtcta tagttgaacc ccatataata tttgcagact catctgcggc
     2461 ttcatatatc ttattagcag catgatcaac ttcaaacaat cccatatcag gtccaccagt
     2521 aatattaaca agtatctttt ttgctccttc aattgatgtt tccagaagtg ggctcttaat
     2581 tgcttccatt gctgcttctt cacatttttc ttcgcctgtt cccctaccaa tacccatatg
     2641 agcaatacct gcatctgtca ttacagtctt cacatcagca aagtcaagat ttatgagtcc
     2701 gggtactgct attaaatcag aaatactttg aactcctttg aaaagaacat tatcagctgt
     2761 attaaaagct tccataatag taattttttt atctgagatc tgtagtaatc tgtcatttgg
     2821 tacgataatt aatgtgtcaa caacagactt aagtcttgca agaccctttt cagcattttc
     2881 ttttcttttt ctgccttcaa atgtgaaagg ctttgttact atacatacag taagaatacc
     2941 catttgtctt gcttcttggg caattatagg gacagctcca gttccggttc cgcctcccat
     3001 acctgctgtt atgaatatca tatcggcacc tttaatattc tcgattataa tatccttaga
     3061 ctcttcagca cttctttctc cgatttcagg tatagatcct gctccaagtc cctttgttag
     3121 tttttcacct atctgaattt ttgtagttgc ttttgaattc ataagggctt gcttatcagt
     3181 attgatagaa ataaattcta ctccttttaa accggaaact atcattctgt caatagcatt
     3241 gtttcctgcg cccccaacac ctataacctt tatctgagca aactgctcca tctccatttc
     3301 gaattgaagc ataatgactc ccctttcgta taagaaaatt ctacttttca ataattaaca
     3361 acattatatc aaacattata ccatcttgta tatacataat aaaaactttt tttcttagtt
     3421 aaattacgac tcaggtgtaa aatatggtgt ttttgaaatt gtaaagtcaa tataaccttt
     3481 tactgtttga ttaatctgag ataatatgtt acacataaca tttgcactat atctcacatc
     3541 ttttgtcata tccataacaa tttcaatcct tttatcatac ttaattgaga ttctgttgtt
     3601 atcctttaat tcgatattgc taattaatgc tcttattgct gtaaaattct ctttatcgta
     3661 tttttctatc tcataacata agactgatac aggctcaaac atgtaagtac aagtgctgat
     3721 atctagtcca atataaaaac aattcaattc gatgtcctca atatagatag aattttcttc
     3781 ttttagcttt gatgcataaa gcactacacc atcataatca cagtacaagt aatattcatc
     3841 atataatatg cagattaccg gttttcttct gctgtaactt atttcaatat catttttccc
     3901 tgtacgattt acgactatct gctttttata tatatctgaa cttaatattt cttctgcttt
     3961 agttaaatga cctgtaaata gattaccctg ttttattagc attgtattta gattatcgcc
     4021 ataccttatt ttggtttgtt ccataatgat ctgtgcatca ttttgtattt gttcatcaaa
     4081 tagtattttt ttcaattcga atgaaggtga aaaaagatac accagtaata ctgatacaag
     4141 aaataatatg attaaaactg attttagcct tctcactttc atggcagaat tataccacat
     4201 attatctgaa cgtaacgttc ttattaaata ggaattgcaa gattcctgcg ataattacgg
     4261 atatggtttt agatataaat ttaaatatcc ctaagttctc ataaagaaaa acaaaaatcg
     4321 tagatcctac cagcattccg ataatgcata cagacgcgaa gtacataaat ttaatagcta
     4381 ttttatctgt ttttttaaaa ttgtattttg aattcaaaaa gaaactaagt gaaataccag
     4441 caaaaacact aataaaattg gataatacat aattaattaa aattacatca gacaaaagat
     4501 aaagtattgc catatctaat attgtggcaa ctgtccctat aagaacatac gtaaaaaata
     4561 tcctatgttt tctaataaac tcaagcattc ataaaatact tagcagtttc tttaagacct
     4621 ttttcaagat tataaacagg ttcccactta agaagatcat ttataaccgt attcttaaga
     4681 taactgtctt taatatctcc ttttcttggt ttggtaaata taggctgttt tgtatatcct
     4741 gtctgtttct tcatggcact aaataattca gatatgtctg tataaacatt tgttgagaca
     4801 ttagcggtaa aatttaaatt actttctaac gctttgacat tcgctttagc aatatcctta
     4861 acataaatga aatctcttgt ttgttttccg tctccgtgaa tgttacagtc ttggtcagat
     4921 accatacggt tcatgaatac agcaactact cccccttcgc cttcagcttt ttgtcttggc
     4981 ccgtatacat tggcatatct taatattcca tatgtcatgt tactgttatt agcgtaaatc
     5041 cttatacatt gctcagacat ctgctttgaa agcccataga aagataatgg attaagagga
     5101 tgagattcat caatgggtaa atattccgga tttccatata ttgctgcgct actagcaaaa
     5161 acaaaccttt taactgaata ctttgtacac atctgaagaa gattgattat accaattaca
     5221 tttgtattta catcaaactg aggtctttcc atggatacag ctactgaaat ctgtgctgca
     5281 tgatgacaaa tcgctttaat ttcatactta ctaaatattt cttctatctg ttcaatatta
     5341 gtaacatcag ccttaatgaa ttttgctttc ggattaaggt taatcatact gccagaaatt
     5401 aaattatcag ctacaattac atcatgtcct ttgttgatca actcatcagt tatatgtgaa
     5461 cctataaaac cgcagccacc agttacaagt atcatactaa atacctaaaa aacctttcat
     5521 tctatcgcaa acatcctgcg cctgcttttc atcaggcatt tccgcaaata ctctcaatag
     5581 tggttctgta cctgaaaatc ttgcaaccag ccatccacca ttattaaaat atactttaag
     5641 tccgtcttcg taacttaatt tttcaattgg taatccaaat tctggtataa gtttgtcttc
     5701 ataaagcatc ttgcttaatt tcggtttttt atcatggtca aaagtatatg aattttctat
     5761 cattatcctg tatccatatt tatcatatat ttcttttaga attacagaca atttcttttt
     5821 tgtcactgcc atcatttcaa caagtaatga tgctgcaaaa ataccatcct tacctgaaat
     5881 atgtcctcta actgtcagac cgccacttga ttcaccccca agcactgcat ctgtctgatc
     5941 tagttttgaa ctaatatgct taaaacccac tggtacctca tggcacataa atccgaaatc
     6001 agcagccata tcatcaagaa catgcgtagt agcaatattt ctaactacat cacctttcca
     6061 tcctttatat ttaacaagat aatagtataa cagcatcatt atctcattgg gatgaataaa
     6121 tctacctttg tcatctatta ctcccaatct atcagcatct ccatcagtac ctaatccgat
     6181 atcataattg tcttcaacca ccattgctga cagtttacca agtgttgctg ctgatggtga
     6241 tggaagtttt cctccaaaca aagtatcatg tctgtcatta attacatcaa catcgcaacg
     6301 aagagttagc agtatggtct gcaatgaagt cttagacact ccatgcatag gatcaagcaa
     6361 gaccttgagt ctggcattaa ttattgcaga tttatctaag aaaaccatta tactgtcaat
     6421 ataatcatta aagggatcaa taatctcaat catatgctca ttaactgctt tatcaaatgg
     6481 catagattta atttctttaa cttttttaat atatccttct atttcatcag taatttccat
     6541 tgcagcatct cttccacctt cggtgaacaa tttaatgccg ttatataaag ccggattatg
     6601 gctagctgtt actgcaattc cataataagc cgtttccttc ttaacttgaa acattattaa
     6661 tggagttgga gaatttttgt atataaaatg aacttttatt gaattcccac aaaaaacctc
     6721 tgcagaccat ttagcacctt cttcagataa aaaacgtcta tcataaccta taataacagg
     6781 tttttcctgt ttattctctt tatgtatctt gtcacaaagt ccctgtacca aaacctgaat
     6841 atttgcctta gtaaactcat ctcctattat ggcacgccat ccacctgtac cgaatttaat
     6901 catttgcccc tctttttctc attattgatt cgactctatc taaatcttcc attgtattta
     6961 caccataaac ttcatcctgt tcatttatca tgcatataac taccttctct ccctctttct
     7021 tcaataaagt aggaaggtct gtcaaataat attcattttg agcattatta ttagtcattc
     7081 tattaagaat atttttgagt ttaattccgt tgaaaagcat tactccaaag aatacttctt
     7141 taatcttttt ttgttgctca tcgcagtctg gatcttctac aatacggcta tatcctttat
     7201 tttcgtctct tataaccctt ccatatggta agtaaacatc gtcgagcata actgaaagca
     7261 ttacacatgc tgctggatca tttttcttta tattatcgtt gtatgccttc aaaaccttct
     7321 tatatgtttt ttttgtaagt aatggcatat caccaaatgt tacaagaaca ggaccatcat
     7381 aatcctcaaa aaactcactt gcacatttta ctgcatgtcc agtacctaat tgttcttctt
     7441 gaactgcata tgagtattta gtccctacat gctcaaatac caaatctttt ttataaccta
     7501 caacaataac aatctcatcg cagaaagaaa gttcttttaa tacataatcg atcattggtt
     7561 tgccatttac aagccttagc gcttttggca atccttcctt ttctgaacca attctttttc
     7621 ctttacctcc agctaatact acagccttca taaactcctc ctaaaattca ggaattatct
     7681 tccatattcc attttcaaaa actgctttcc ataaaacagt cttgtaaatc tcagttacat
     7741 catcaccttt agtatacttc aaagtcacgc ttacttcagc acttttttca ttatcattag
     7801 tgataacata ttcacctatt gtatatccaa gtattttggt ctgttttata actggaaatt
     7861 cattttccag ccattcgtca taatcatttt ctctataatc gattggagta agaacaaata
     7921 gcatatattg attcagatta ttagtactgc ttagcactga taggtattct tttgcagcca
     7981 ttgcaggagt tcttccaatc gggtctttgt ctccatcagc taatggataa cctgatatgt
     8041 catttgggaa tatgtatttt gatgcatcgt aactaattcc attattccaa aacatatatg
     8101 aaaaggaacc tagttcgcgc aatgctttta cttgcccgta tatctgttca tatccgtaat
     8161 agtaaattga gtctggctgg taaagatatt tagcagtaaa atcctgtatc cagagtctga
     8221 ctttaccatt atcattaaaa gctgagtttt tttcaattga atctttcatt gcacctagaa
     8281 ccattccgta aggattcata tcaggatatt caaatccata ccatcccgtc gtgtaatgac
     8341 ttggataaat catagggcaa atatagtcta ctac
//
LOCUS       DAOSXM010000088         8278 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2111
            NODE_5551_length_8278_cov_11.515384, whole genome shotgun sequence.
ACCESSION   DAOSXM010000088 DAOSXM010000000
VERSION     DAOSXM010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864178
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 8278)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8278)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:24:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,261
            CDSs (total)                      :: 2,229
            Genes (coding)                    :: 2,218
            CDSs (with protein)               :: 2,218
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 3 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8278
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5551_length_8278_cov_11.515384"
                     /isolate="AMR_MDS_2111"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            189..1022
                     /locus_tag="PLQ68_09290"
     CDS             189..1022
                     /locus_tag="PLQ68_09290"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HXK72887.1"
                     /translation="MKKSKYRWIYIIAFILVALITVFFIFGAERSSYFEALDATHPDA
                     KWLINQPIAHRGYHNEKTAPENSLEAFSEAIKKGYAIELDVLLSKDDYVIVVHDKNLE
                     RLTGADIDVSDMTLDQIKHLTLLDTQISIPTLEEALELINGKVPILLEIKSTGLNHQL
                     DELTYSIIKGYEGEIAVQSFNAASIRWFMKNAPDITRGLILSGNIKFDIANKFIDNLY
                     MLYSNPNFVSYKYNNLLKTDFSELRKDGLIVLGWTVNESMLNTREFVQYCDNIILEAN
                     K"
     gene            1019..1444
                     /locus_tag="PLQ68_09295"
     CDS             1019..1444
                     /locus_tag="PLQ68_09295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72888.1"
                     /translation="MMVKGRILLGYDDKPKDKKKVKYSDWLIILMSTSPIWLSILLII
                     VVAVSVFFVALVIAFIAVGFSSVMLFVYSAFLLLDDLWPSVLKMGACLFASGITIIIV
                     SFLFRGFKEFFILIKKSYLKIKKFLMLKKLEGQKDEQNA"
     gene            1428..2351
                     /locus_tag="PLQ68_09300"
     CDS             1428..2351
                     /locus_tag="PLQ68_09300"
                     /inference="COORDINATES: protein motif:HMM:NF024743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HXK72889.1"
                     /translation="MSRTLKALIIGGIFCVLGLVTFFIGYHFNGYRLDNIFAYEAPVE
                     DPLVKKSYELTDPDDLVLISISTKRMPITIKAHDEDQIIIEYLENQDQLFEITDNNGK
                     LFFKKQDTGFFEEYISSNIMAYLKKEEPEGIVLYIPKTYDGELYASTSFGDISIPDAL
                     SFSLIKKIDFKNSFADVNINSLSGNILNLKVARGNINCSDIKFKSITFICTSCNVQAD
                     YISSENLDIIAKNGSIDASNIMSDNIDVDADGADIHLRLLAYSNQYNIKINEDHKSIL
                     NFEQTSDFQTDKEIVIKTNGSMINITFTLDK"
     gene            2445..3272
                     /locus_tag="PLQ68_09305"
     CDS             2445..3272
                     /locus_tag="PLQ68_09305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014253475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HXK72890.1"
                     /translation="MYKILEKKDLNNEVVYMKVDAPAVAKKCEPGQFIILRVNEQGER
                     IPLTIEDYDREEGWVSIIFQKVGKTTYLLGTLNEQDYILDFVGPLGTASHTDGMKRVC
                     VIGGGLGCAIAFPQAKKLYNNGAHVDIIAGFRNKDLVILQEEMKKVSTNYYLMTDDGS
                     AGEKGLVTEKLKKLIEQGSNYDTVIAIGPVIMMKFVSLLTKEYGIKTIVSMNPIMIDG
                     TGMCGGCRLTVGGETKFACVDGPDFDGHLVDFDSVLQRLSMYKEEENHKCNLFRGIE"
     gene            3272..4672
                     /gene="gltA"
                     /locus_tag="PLQ68_09310"
     CDS             3272..4672
                     /gene="gltA"
                     /locus_tag="PLQ68_09310"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013297645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004355 - glutamate synthase (NADPH)
                     activity [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HXK72891.1"
                     /translation="MAKINMTLVKTPMPEQDPMVRNCNFSEVSTGYTHQMAVEEANRC
                     LNCKHKPCVAGCPVNIDIPNFIKSIVNENIEEAYNIITKTNSLPAICGRVCPQESQCE
                     LPCVRGIKGEPVAIGRLERYVADYMMAKETFTTDVQKPSVKKNKKVAIVGSGPAGLAA
                     ASDLCDMGYDVTIFEALHKAGGVLVYGIPEFRLPKEIVSKEIERLKTKGVKILLNMVI
                     GKTISIAELHDEEGFDAIFVGTGAGLPNFMNIPGENLNGVYSANEFLTRVNLMKAYDP
                     AYDTPVYFGKHVAVVGGGNVAMDAARAAKRLGAEHVSIVYRRSEKEMPARIEEVHHAK
                     QEGIDFHLLTNPTKILGEKGWVKGMECVKMQLSEPGPDGRRRPEVIPGSEYILDVSSV
                     IIAIGTSPNPLIKNTTSGLETNKWGCIIADQETGQTTIPYIFAGGDVVTGAATVILAM
                     GAGKKAASAIDKYLDK"
     gene            4700..5863
                     /locus_tag="PLQ68_09315"
     CDS             4700..5863
                     /locus_tag="PLQ68_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006778268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HXK72892.1"
                     /translation="MKFMDQLFETVLPLGELSIIGMHGCEEITKKIDWYLKQWTCDKD
                     PDHTFIIDAICPRFGTGEAKGVILQSVRGHDLFIICDAFNYGVSFEMYGMQVPMSPDD
                     HFADLKRLIAATNGKARRITVIMPMLYEGRQHKRKYRESLDCAIALQELVDMGVDNII
                     TFDAHDPRIQNAIPLNGFESVPVAYQMLKRLTNTFPDIIFDKEHTMVISPDEGGMNRA
                     IAFASMIGLDLGMFYKRRDYSIVEHGRNPIVSHEFLGDNVEGKDVIVVEDIIASGESA
                     LDIFYQLYEKKARRVFMCCSFGLFCNGFDEFDKAYEQGAVTAVLTTNLVYRSPELLKR
                     EWFKEVDMSKYMSYIIDTLHHDASMSNLLNPVDRIDKLLEKIKEKQEEDGLIK"
     gene            complement(5961..7139)
                     /locus_tag="PLQ68_09320"
     CDS             complement(5961..7139)
                     /locus_tag="PLQ68_09320"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016279976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HXK72893.1"
                     /translation="MSKINVNYNNLKQSYLFKDIATKVAAYKKANPDKKVISLGIGDV
                     TLPLAKKVVEALKKASDEMGNAETFKGYAPDQGYDFLREKIALHYKKRNVDLDISEIF
                     VSDGAKSDVGNITEIFSRDNTVLIPDPVYPVYMDTNVMDGRKIIFSDANADNDFLAMP
                     DERHADMIYICSPNNPCGSVYTKEQLKKWVDYAIKNDAVILFDAAYEAFITNEKLPRS
                     IFEIDGAEKCAIEFCSFSKLAGFTGTRCAYTIIKKELKLDGFLLHDMWVRRQSTKLNG
                     VSYVIQRAAEAVFTDEGMAQCKNNIKYYLDNAKLISALLKEKGIYHTGGTNSPYIWLQ
                     CPNNMKSWEFFDLLLNKISVVGTPGSGFGTNGEGYFRLTSFGSRENTIEAVERLKGIL
                     "
     gene            complement(7160..8041)
                     /gene="dapF"
                     /locus_tag="PLQ68_09325"
     CDS             complement(7160..8041)
                     /gene="dapF"
                     /locus_tag="PLQ68_09325"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HXK72894.1"
                     /translation="MRFTKMHGAGNDYIYVNCMDCKIDNPSSLAVKVSDRHKGIGSDG
                     LVLIQPSVNADFYMRMFNADGSEGAMCGNAIRCVAKYVYDNKLTDKLNLDIETKSGIK
                     HLKLFLDKEGLVDSAQVDMGLAYLKREQIPMICDETNKDETYVNKPITVTKKEYNVTC
                     VSMGNPHCVIFMNDKKNTDNLKNQLELLDLDRIGPDFENNSIFPDRVNTEFVEVIDKT
                     TLKMRVYERGSGETMACGTGACATVVAAVLNGICDRNKTVTVILKGGTLEIAYLDDGR
                     VIMKGEAVHVFDTEINI"
BASE COUNT         2755 a         1396 c         1500 g         2627 t
ORIGIN      
        1 ataaaattga aggcaaataa tcgcttccta atacaaaaca cggaaactca acgataatat
       61 taatgaaaat acgactttta taacataaat atgagtaata tatatgcgag aactaaaaac
      121 acctctattc cattgataat tgcatatgat ataattgaaa gagaaattgt tgttggtaaa
      181 ggaaaaaaat gaaaaaatca aaatatagat ggatatatat tattgctttt atattagtcg
      241 cactgattac agtgttcttt atttttggag cggaaagatc ttcttacttt gaagctttag
      301 atgcgacaca tccagatgcc aaatggctta ttaaccagcc tatagcacac agaggttatc
      361 ataacgaaaa aactgctcct gaaaattccc tagaagcttt ttctgaagct atcaagaaag
      421 gatatgcaat cgaacttgat gttctattgt caaaagacga ttatgttatc gttgttcatg
      481 acaaaaacct ggaaagactt acaggggcag atatagatgt ttcagatatg acattagatc
      541 agataaaaca tttaacttta cttgatacgc aaatatcaat accaacttta gaggaagcgc
      601 tagagctcat aaacgggaag gttcctatac ttttggaaat taaatcaaca ggtctcaatc
      661 atcagctgga tgaacttacc tattctataa tcaaaggcta tgaaggagaa attgctgttc
      721 aatcttttaa tgccgcaagc atacgatggt tcatgaaaaa tgctccggat attacaagag
      781 gattgatttt atctggtaac ataaagtttg atattgcaaa taagtttata gataacttat
      841 atatgctgta ttctaatcct aactttgtat catacaaata caataatctt ttaaaaactg
      901 acttttcgga acttagaaaa gatgggttaa tagtacttgg atggacagtg aatgaaagca
      961 tgctaaatac tcgtgagttc gttcaatact gtgataatat aattctggag gcaaataagt
     1021 gatggtaaag gggagaatcc ttttaggcta tgacgataaa cctaaagaca aaaagaaagt
     1081 aaaatactct gactggttaa taatcctcat gtcaacatca cctatatggc tttctatact
     1141 tttaataatt gtagtggcag tatcagtttt ctttgtggct ttggtaattg catttatagc
     1201 tgttggtttc agttctgtta tgttattcgt atattcagct tttcttcttc tagatgatct
     1261 atggccatcg gttcttaaaa tgggagcatg cctttttgct tcaggtataa ctataatcat
     1321 tgtctcattc ctgtttagag gatttaagga gttcttcatt ctaattaaga aaagttattt
     1381 aaagataaag aagtttttaa tgctgaagaa attggaggga caaaaagatg agcagaacgc
     1441 ttaaagccct cataatcggc ggcatattct gtgttttagg acttgtaact ttttttatag
     1501 gctatcactt caacggatac agattagaca atatattcgc atatgaggct cctgttgaag
     1561 atcctcttgt aaaaaaaagt tatgaattaa ctgacccaga tgatcttgtt ttaataagta
     1621 tatcaacaaa aagaatgcca attaccatta aagctcatga tgaagatcaa ataataatag
     1681 aataccttga aaatcaggat cagcttttcg aaataactga caataacgga aaactttttt
     1741 tcaaaaaaca agatacaggt ttttttgaag aatacatatc atccaatatt atggcttatt
     1801 tgaaaaagga agaaccggaa ggaattgttt tatacatacc gaaaacatat gatggagagc
     1861 tttatgcatc tacttcgttt ggagacatat ctattcctga tgctttatca ttcagcctga
     1921 ttaagaaaat tgattttaaa aattcttttg cagatgtaaa tattaattct ttatcaggca
     1981 acatactaaa tctgaaggtt gcaagaggaa acatcaactg ctccgatatt aagtttaaaa
     2041 gcattacctt tatatgcaca tcttgtaatg tgcaagctga ttacataagt tctgaaaatt
     2101 tagatattat tgcgaaaaat ggcagtattg atgcttcaaa tataatgtca gacaacattg
     2161 atgttgatgc agatggagca gatattcacc ttagattact tgcatattca aatcagtata
     2221 atattaaaat caatgaagat cataaatcta tattgaattt cgagcagaca agtgattttc
     2281 agacagataa agagatagtt ataaaaacaa atggttctat gataaatatt acctttacat
     2341 tagacaaata agataaatga aatataaata aacactgttt gattgaaaat gacctttttc
     2401 agtgttatta tttattgagt actaatagaa tggtggtgct ttaaatgtat aaaatactgg
     2461 aaaagaaaga tttaaacaat gaagtcgtat atatgaaagt tgatgcccca gctgtagcaa
     2521 agaaatgcga accggggcag tttattattc ttcgagtaaa tgagcaaggt gaaagaattc
     2581 ctctcactat tgaagattat gaccgtgaag aaggctgggt aagcataata ttccaaaaag
     2641 ttgggaaaac aacttatctt cttggaacac tgaatgaaca agattacata ctggattttg
     2701 taggtccttt gggcacggcc tctcatacag atggaatgaa aagggtttgt gtaattggag
     2761 gaggattagg atgcgctatc gcttttcctc aggcaaaaaa actttacaat aacggtgctc
     2821 atgtagatat tattgctggt ttcagaaaca aagatttagt aattctacag gaagagatga
     2881 aaaaagtaag tacaaattac tacctcatga ctgatgacgg atcagctgga gaaaaaggac
     2941 ttgttactga aaaactaaaa aaactgattg aacagggttc aaattatgat accgtaattg
     3001 ccattgggcc agttataatg atgaaattcg tttcattact aactaaagaa tatggtatca
     3061 aaacaatagt gagtatgaat cctattatga tagacggtac tggtatgtgt ggaggatgca
     3121 ggcttacagt tggaggagaa acaaagttcg catgcgtgga tggaccggat tttgacgggc
     3181 atcttgtgga ttttgacagt gttcttcaga gactttcaat gtataaagaa gaagaaaatc
     3241 ataaatgtaa tttatttaga ggtattgaat aatggcaaaa atcaatatga ctttagtaaa
     3301 aactcctatg cctgaacagg accctatggt ccgtaactgc aatttttctg aagtttcaac
     3361 cggttatact catcagatgg ctgttgaaga agctaataga tgtttaaact gtaaacacaa
     3421 accctgtgtt gcaggatgtc ctgtaaatat agatattccg aattttatta aatccatagt
     3481 taatgagaat atcgaggaag catataacat tattacaaaa acaaattctc tgccagctat
     3541 ctgcggaaga gtatgtccgc aggaatccca gtgtgagctt ccctgtgtca gaggtataaa
     3601 aggtgagcct gtagccattg gcagactgga aagatatgtg gccgactata tgatggcgaa
     3661 agaaaccttt acaactgacg tccaaaagcc ctctgtaaag aagaataaaa aagtagcaat
     3721 agtcggaagc ggtcctgcag gtcttgcagc ggcatctgac ctttgcgaca tgggatatga
     3781 tgtaactata tttgaagcgc ttcataaagc tggcggtgtt ttagtatatg gaataccgga
     3841 attccgactg cctaaagaaa tagtaagcaa agaaattgaa agacttaaaa caaaaggtgt
     3901 aaagatattg ttgaatatgg ttatcggaaa gactatatcc attgctgaat tgcatgacga
     3961 ggaaggtttt gacgccatat tcgttggaac aggagcagga ttgcctaatt tcatgaatat
     4021 ccctggagaa aaccttaatg gcgtatactc tgctaacgaa tttttaacaa gagtcaatct
     4081 tatgaaagca tatgatcctg cttatgatac tcctgtttat tttggaaaac atgttgctgt
     4141 agtaggtggc ggaaacgttg cgatggacgc agcaagagca gcaaagaggc ttggagctga
     4201 gcatgtaagc atagtataca gaagaagtga aaaagaaatg cctgcaagaa ttgaggaagt
     4261 tcatcacgca aagcaagaag ggatagactt tcatctttta acaaatccta caaagatact
     4321 tggagaaaaa ggatgggtaa aaggaatgga atgcgttaag atgcagcttt cagaaccagg
     4381 acccgatgga agaagaagac ctgaggttat acctggttca gagtatattc tggatgtatc
     4441 ttctgtaatt atcgctatag gaacaagccc caacccctta ataaaaaata ccacatcagg
     4501 attagaaaca aacaaatggg gctgcattat tgcagaccag gaaactggtc agacaaccat
     4561 cccttatata tttgctggtg gagatgtagt gactggagca gcgactgtaa ttttagcaat
     4621 gggagctggc aagaaggcgg cctcagctat tgataaatac ctagacaagt gatatcatta
     4681 tcaaagctta aaaggaaggg tgaaatttat ggaccagcta tttgaaaccg tgcttccctt
     4741 gggggagtta tctataattg ggatgcatgg atgcgaagaa attactaaga aaatcgattg
     4801 gtatctaaaa cagtggacat gcgataaaga tcctgatcat acatttatta ttgatgctat
     4861 atgtcccaga ttcggaacag gagaagcaaa aggagttatc ttacaatcag taagaggaca
     4921 tgaccttttc ataatatgcg atgctttcaa ttacggagta agttttgaga tgtatggaat
     4981 gcaggtacca atgagtcctg atgaccattt tgccgatttg aaaagactta tcgctgcaac
     5041 aaatggaaaa gcaagaagaa taacagttat aatgccaatg ctttatgaag gcagacaaca
     5101 taaaagaaag taccgtgaat cattagattg cgctatcgca ttacaagagc tcgtggacat
     5161 gggtgttgat aacattatta catttgatgc acatgaccct cgaatacaga acgcaatacc
     5221 tctaaacgga tttgaatcgg ttccggtagc atatcagatg cttaaaagac tgacaaatac
     5281 ttttccagat attatttttg acaaagaaca tactatggtt atttctcctg atgaaggcgg
     5341 aatgaacaga gctatagcat ttgcttccat gattggcttg gatttaggca tgttctacaa
     5401 aagaagagat tactctattg tagagcatgg aagaaaccca atagtatctc atgaatttct
     5461 aggagacaat gttgaaggta aagacgtgat agttgttgaa gacattatag cctctggtga
     5521 atcagcactt gatatctttt atcagcttta tgaaaagaaa gcaagaagag tctttatgtg
     5581 ctgctctttc ggcttattct gcaatggatt cgacgaattt gacaaagctt atgaacaagg
     5641 agcggttact gctgtgctta caaccaatct tgtatacaga agtcctgaac tattaaaaag
     5701 agaatggttc aaagaagttg atatgtcaaa atatatgtcg tacataattg atactcttca
     5761 ccacgatgca tcaatgtcca atctcttaaa tcctgttgac agaattgaca agcttcttga
     5821 aaaaattaaa gaaaagcaag aagaagatgg attaataaaa tagtcagtaa attactaaat
     5881 tacagatgcg taatataaaa tatacacaga attacaaaaa acagcgctta tgcgctgttt
     5941 ctttttatat gccttactat ctataatatg cctttcagtc tttccactgc ttcaattgta
     6001 ttttccctgc ttccaaaaga ggtaagcctg aagtatcctt ctccgttagt tccaaaaccg
     6061 cttcctggtg ttcctacaac actaatctta tttaataaaa gatcaaagaa ttcccatgat
     6121 ttcatattgt tcggacattg cagccagata tatggtgaat tagttcctcc ggtgtgatat
     6181 attccctttt ctttgagtaa tgcagatatg agttttgcgt tatcaaggta atatttaatg
     6241 ttattcttac actgagccat tccttcatca gtaaatacag cctcagctgc tctttgaatt
     6301 acataagaca ctccgtttag cttagtgctc tgtcttctta cccacatgtc atgtaataag
     6361 aaaccatcaa gttttaattc ttttttaata attgtatacg cacatcttgt tccggtaaat
     6421 cctgccagtt tggaaaaact gcagaattct atcgcacatt tctctgctcc gtctatttca
     6481 aagatactgc gaggaagctt ttcatttgtt atgaaagctt catatgctgc gtcgaacaaa
     6541 ataaccgcat catttttaat tgcataatct acccattttt taagctgttc tttggtatat
     6601 acgcttccac atggattatt aggagaacag atatatatca tatcggcatg cctttcatcc
     6661 ggcattgcta aaaaatcgtt atctgcattc gcatctgaaa aaattatttt tcttccatcc
     6721 attacatttg tatccatata tactggataa accggatcag gaattaaaac agtattatct
     6781 cttgaaaaaa tctctgtaat atttcctaca tcactttttg ctccatctga tacaaatatt
     6841 tcacttatgt ctaaatcaac atttctcttt ttgtaatgta aagcaatttt ctctcttaag
     6901 aaatcataac cctgatcggg tgcataacct ttaaatgtct ctgcattccc catttcatca
     6961 gatgctttct tcaaagcttc aactaccttt ttggctaaag gaagcgtaac atcccctatt
     7021 ccaaggctta taaccttctt atctggattg gcttttttat atgctgctac ctttgttgct
     7081 atatccttga aaagataact ctgtttcagg ttgttgtaat ttacatttat ttttgacatc
     7141 taattctcct tttcttttgt tatatattta tctctgtatc aaatacatga acggcttcgc
     7201 ctttcataat aactcttcca tcatcaagat atgctatctc aagtgttccg ccttttaaaa
     7261 ttactgttac tgtcttgttc ctatcacata tgccattaag aactgcagca acaactgttg
     7321 cacaggcacc tgttccgcag gccatggttt cacctgagcc tctttcataa actctcattt
     7381 tcagagtggt tttatctata acttcaacaa actctgtatt aaccctgtca ggaaatatgc
     7441 tgttattttc aaaatcaggt ccgattctat caaggtccag taattccagt tgatttttca
     7501 aattatccgt attcttttta tcattcatga atatgacaca atgaggatta cccattgata
     7561 cacaggtcac attatattct ttttttgtta cagtaatggg tttgttaaca tatgtctcat
     7621 ctttgtttgt ttcatcacaa atcatgggta tttgttctct tttaagataa gccaacccca
     7681 tatccacttg tgcagaatcc actaagcctt ccttatcaag aaaaagcttt aggtgcttta
     7741 tcccgctttt cgtctctata tccagattta acttgtctgt aagcttatta tcatacacat
     7801 actttgcaac acatcttatt gcattcccgc acatcgcgcc ttctgatccg tctgcattaa
     7861 acattctcat gtagaagtct gcatttactg atggctgaat aagcaccaga ccatctgaac
     7921 ctataccttt gtgcctgtcg cttactttta ctgctaaaga ggaaggatta tcaattttac
     7981 aatccataca gtttacgtat atatagtcat ttcctgcacc atgcatttta gtgaatctca
     8041 ttgttgttct ccctttgacc tattatacat aaaaatacag gttgggtaaa taacttaacc
     8101 attatcttaa agttttgcga tttgattatt ctaatatttt tagtgctttt tcgaaggagc
     8161 accctattat cctaaagaat cattgtctaa actcttggtt tctctataat tttatgattt
     8221 actaatattc aatgttagtt tatcataagt cgaaggaatt tccatgtata taaagaga
//
LOCUS       DAOSXM010000089         8222 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2111
            NODE_5617_length_8222_cov_11.160402, whole genome shotgun sequence.
ACCESSION   DAOSXM010000089 DAOSXM010000000
VERSION     DAOSXM010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864178
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 8222)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8222)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:24:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,261
            CDSs (total)                      :: 2,229
            Genes (coding)                    :: 2,218
            CDSs (with protein)               :: 2,218
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 3 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8222
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5617_length_8222_cov_11.160402"
                     /isolate="AMR_MDS_2111"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            77..739
                     /locus_tag="PLQ68_09330"
     CDS             77..739
                     /locus_tag="PLQ68_09330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72895.1"
                     /translation="MENCLIDIYDRPEFMHKMMDKFTRYHEEIISQYEKLNILETDAY
                     YLHCTPACTYELPIKDVTKEKVSSKEVWGRGMAQIFAVVSPDMHNEFDIQYMKRLFDQ
                     CGLTYYGCCEPLDKKIDILREFSNLRRISITPWADVDIAADAMGKDYVLSAKPNPAYV
                     SQSVFDPLPVKDEIRKILLACKKNNTPCEFVIKDISTINKDVSHLTNWVKTVSETIDR
                     YW"
     gene            complement(742..1806)
                     /locus_tag="PLQ68_09335"
     CDS             complement(742..1806)
                     /locus_tag="PLQ68_09335"
                     /inference="COORDINATES: protein motif:HMM:NF014477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HXK72896.1"
                     /translation="MHTSKRTIYIKHIIIILIVSSLLLLACCSSQNPLYISRKNGMNT
                     VSFLAMDTIIAISVDSQVTDKKLENAYYEIQQFENAYSAYITDSELYEINQSSSKKTE
                     ISEDLYNQILISQKVSEMTDGAFDITVLPLINLWGFSNGEYKVPKEAEIIKALETIGY
                     EKIHLDDQAMLSAQGTNIDLGAVAKGYIGQKIADKLQEEGSSFGILSLGGNMQLYGKK
                     PDGSNWNIAIQDPLDESSIIGIISTTDCSVITSGSYQRYFIENDIKYHHILDPKTGYP
                     ADNGLISVTIVAKDGAYADALSTALFVTGLEKSIEIWKEYNDFEAVFITEDKSVYITE
                     GLKDRFTPESEDFLFDYVYK"
     gene            complement(1863..2420)
                     /locus_tag="PLQ68_09340"
     CDS             complement(1863..2420)
                     /locus_tag="PLQ68_09340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014117833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HXK72897.1"
                     /translation="MKYYDINIAGIDRSLPLCRVTDSLYIAAFVVFGDVELTIACAKR
                     LLELAPEYDYVITAESKGIPIVHEMARQSGKNKYFLARKAPKLYMTGVFEVDVKSITT
                     SSLQKLYLDTADAEFMKGKRILIVDDVISTGGSLRAVEELVLRAGGIVAGKMAVLAEG
                     DAIYRNDITYIAPLPLFNPDGTIKR"
     gene            2913..3383
                     /gene="mscL"
                     /locus_tag="PLQ68_09345"
     CDS             2913..3383
                     /gene="mscL"
                     /locus_tag="PLQ68_09345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010536866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HXK72898.1"
                     /translation="MAEKKKGSLMSEFKAFITKGNVIDLAVAVIIGGAFGKIVSSFVN
                     DILMPIIGLALGKVSFNDLKWVLATDSEGNITSSVNYGTFIQFIIDFLIIALSVFLII
                     RLIAVAKQKAEEAKRKKEALEKAEEAPPVPPAPPVPTKEEVLLTEIRDLLKEKK"
     gene            3418..3753
                     /locus_tag="PLQ68_09350"
     CDS             3418..3753
                     /locus_tag="PLQ68_09350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72899.1"
                     /translation="MDGFDDFSKKLENAGKKLFKKTKEFADTTKLSIKLKDLEIDLEN
                     LFQQAGKKAFKGEDTKEVFDEITNLQGQIESLKAEIADAKDMKICPNCKSEMPKEAVA
                     CMKCGHKFE"
     gene            3757..4236
                     /locus_tag="PLQ68_09355"
     CDS             3757..4236
                     /locus_tag="PLQ68_09355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase family protein"
                     /protein_id="HXK72900.1"
                     /translation="MKRIDYISWDEYFMGIAILSSKRSKDPNTCVGACIVDEFNKVLS
                     LGYNGMPIGCSDDVFPWEREGQALDTKYPYVCHAELNAILNYDGYGLKGSTLYVTLFP
                     CNDCAKAIIQKGIKKVVYLDDKYSDQDTTKASKLMFDAAKVSYVKYELRGKDITINL"
     gene            complement(4223..5128)
                     /locus_tag="PLQ68_09360"
     CDS             complement(4223..5128)
                     /locus_tag="PLQ68_09360"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HXK72901.1"
                     /translation="MKHLFIINPMSGNKKATAYVPEIEKYFKSSEQEYSIVYTKYAMH
                     AKEICEEYAKKGGYRIYIAGGDGSINEAANGVAGYDCPIGILPAGSGNDFIKCLMDYS
                     NKTDIVRRTIEGEVIKIDAIKLFDEEKFNYCINILSLGVDAEAAYNSTYYTRKYGITG
                     VPAYLLGFFKALNQKKTKFKVKITIDGQVIHNGYVMLAACTNGKYYGGGFIPVPHTSF
                     SDGIIDMCIVEDKNLAFVFSVLPKYMKGKHAGIDGITFPKGKSISLESEDMLKVNIEG
                     EIVERKSVRMEVVPGLINVIKPRDL"
     gene            5270..7759
                     /locus_tag="PLQ68_09365"
     CDS             5270..7759
                     /locus_tag="PLQ68_09365"
                     /inference="COORDINATES: protein motif:HMM:NF013256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HXK72902.1"
                     /translation="MKNLHLVCNAHIDPVWLWELEEGVAETLSTFRVSAQFCEEYDGF
                     IFNHNEALLYQWVEKYEPSLFKRIQKLVKDKKWHIMGGWFIQPDGNMPSGESFVRQIL
                     EGRNYFMEKFGTFPTTCINFDAFGHTRGLVDILKNSGYDSYLFCRPEPDMIELPDEDF
                     LWEGFSGDTIMCNRAYNSYESHRGEADQKIKGWMENNRNRPVGMVLWGIGNHGGGPSR
                     VDFDNISKMAKEEKEFNIIHSTPEAYFEQMKEKQLPIYKGILNPRYIGCYTSQIRIKQ
                     LHRKLENELYMTEKMAAHAASSKLMKYPQDNIQQATKDLLLMEFHDILPGSAIQPVEN
                     YSISLAGHGLEELGRVKTQAFLSLCSGQKVANDGEIPLLVYNPHPYEIPYIVECEFQL
                     ADQNKNKKIFANPVVYQNGRKLPCQVEKEASNFNVDWRKKSVFYAKLKPGQINRFDAR
                     IEMIDSKPVPVIRQTKDKFIFKTKEITVHISKLNGTIDKYEVNGVDYIAKNAFLPVVI
                     KDDENSWAHKEKSFRNTEGYFKVMDINEGNVFSGIIDEKQIESVRAIEDGSVRTVIEA
                     VMKYDNSFICLRYHLPKKGTQIKVTVKVYWNEKMKMLKLSVPTVIDNPSYTGQQAYGS
                     EKLLTNGDEMVSHKWNVLYNRQKALSIINTGTYGSDVLDNEMRISLLRSAGYSAGKSD
                     FSVRNKYIMPQDRFSPYMDQGERDFEFYLDASDTKTRLEQIEREAAVCNEKPMALSFF
                     PSGQGKKIKPFAILSDDVITMPVFKRSEDKKNYIIRLFNPTDKKRVTTLKLPMLRWEN
                     EFELDAYQLKSYKLDVSTKRVVETDLVERNK"
     gene            7831..>8222
                     /locus_tag="PLQ68_09370"
     CDS             7831..>8222
                     /locus_tag="PLQ68_09370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72903.1"
                     /translation="MHKQKSSKRVISLLTCEFFDKRLENEYRQYDISNAIKYVGPILL
                     MLGISFFLFIIPDFFIIQDKKTLLVIFLNRLVFLALITLFYFKIKTAKTYEFYYRWIT
                     FYEVIACIFFMVTLYNYENPNLFIQSFG"
BASE COUNT         2793 a         1287 c         1455 g         2687 t
ORIGIN      
        1 cttggaagct catcccacat attaaacata agctggtatc ccccaagttt gacatgagct
       61 tccaagatat catggtgtgg agaactgtct tatagacata tacgacagac ctgaatttat
      121 gcataagatg atggataagt tcacaagata tcatgaagag attataagtc agtatgaaaa
      181 gctaaatatt ctggaaactg atgcatatta tcttcactgc acacctgcat gtacttatga
      241 gctaccaata aaagatgtaa caaaagaaaa ggtatcttca aaggaagtct ggggaagggg
      301 aatggcacag atttttgctg tagtatctcc tgatatgcat aatgaatttg atatccaata
      361 catgaaaagg ttatttgacc agtgcggact tacatattat ggttgctgtg aaccgcttga
      421 taagaaaata gatatattaa gggaattttc gaatttgaga agaatatcga taactccatg
      481 ggcagatgtt gatatagcgg cagatgcaat gggaaaggat tatgttcttt ctgccaaacc
      541 aaatcctgct tatgtttctc aatctgtatt tgaccctctg cctgttaagg atgaaattag
      601 aaaaatactt ttagcatgta aaaaaaataa cacgccatgc gagtttgtta taaaggatat
      661 ttcaactata aataaagacg tttcgcatct gaccaattgg gttaaaacag tttcagaaac
      721 tatcgaccgt tactggtaag tttatttata tacatagtcg aataaaaaat cttcagattc
      781 tggcgtgaat ctgtctttta gtccttcagt tatatagaca cttttatcct ctgttatgaa
      841 tactgcttca aaatcattgt actctttcca tatttcaata cttttttcaa gtccagtgac
      901 aaaaagagct gtagataaag catcggcata agctccgtct tttgcaacaa ttgttacaga
      961 aatcagtccg ttatcagcag gatatcctgt tttagggtct agtatatgat gatatttaat
     1021 atcattttct ataaaatatc tttgataaga acctgatgtt ataactgaac agtcagtagt
     1081 tgatatgatt ccgattattg aagattcatc taatgggtcc tgtatcgcaa tgttccagtt
     1141 actgccatct ggttttttcc cataaagctg catattaccg cctaaagaca aaataccgaa
     1201 gctggatcct tcttcttgaa gcttatctgc aatcttctga cctatataac cttttgcaac
     1261 agcacctaaa tcaatatttg taccttgagc tgaaagcatt gcttgatcat ctaaatgtat
     1321 tttctcataa ccgatagttt ccagtgcttt aattatttcc gcttcctttg gtactttgta
     1381 ctctccattt gaaaagcccc ataagtttat taatggaagt actgtgatgt caaaagctcc
     1441 atcagtcatt tctgatactt tttggctgat gagtatctga ttatataaat cttctgatat
     1501 ttcagttttt ttagaagaac tctgatttat ttcatataac tcactatctg ttatgtatgc
     1561 tgaatatgca ttttcaaatt gctgtatttc ataatatgca ttttcaagtt ttttatcagt
     1621 cacctgacta tcaactgata ttgcaattat tgtgtccata gctaaaaaag aaacagtgtt
     1681 cataccattt tttctggata tataaagggg attttgtgaa gaacaacaag ctaataataa
     1741 aagagatgat acgataagta ttataattat atgttttata tatatagttc tttttgatgt
     1801 atgcataaaa taaaaatact ccttaagaaa aaaggccgtt tttgcggcct tttaaagctt
     1861 tattatcttt taattgtccc atcaggatta aacaatggca atggtgctat gtatgtgatg
     1921 tcatttctgt atatagcatc gccttcagct agaactgcca tctttccagc tacaattcct
     1981 ccagcacgca agactaactc ttcaactgct cttaaagatc caccagtaga aataacatcg
     2041 tctactatta atatgcgttt tcctttcatg aattcagcat ctgccgtatc aagataaagt
     2101 ttttgaaggc ttgatgtagt tatggattta acatcaactt caaatactcc ggtcatatat
     2161 agtttcggag cctttcttgc cagaaaatat ttattttttc cactctgcct cgccatttca
     2221 tgtacgatag ggattccttt tgattcagca gttataacgt aatcatattc tggagcaagt
     2281 tccaataatc tttttgcaca tgcaattgtg agttcaacat ccccgaacac tacaaaagct
     2341 gctatgtata agctatctgt aactctgcaa agcggtaaac ttctatcaat tcctgcaatg
     2401 tttatatcat agtatttcat attcacgcat cctttcaatg cttcttttca acatatatat
     2461 tatacaacaa atgtttatct gtataaagac aaaaagcttt attgaaaaac taacagtgtt
     2521 tatgcatttt agttatataa aattatcttt acttataaat tattgtctta ttgcttcagt
     2581 tatatttttt cattttattc tgaatagcaa gtattttcta taccttttat aagatcatta
     2641 tcaatatgtt attaaaattg tgaacatttc tttaactttt gaattatagt attgaaatgg
     2701 gttaataaaa tgatataatt aaaccacaat gaagctggta aaggtgcaaa cctttttctt
     2761 ggccggctga gtctttgaag gatctgaaat cggcgttctg caggattgat gatctttctt
     2821 ctttttgaaa caatatatta ataaacctgc tgcaaatatt acttttaata gtaaaattaa
     2881 agtgtcagta aaatataaga ggaggaaaga acatggctga aaagaaaaag gggagtctga
     2941 tgagtgagtt taaggcattt attactaaag gaaatgtaat tgatttggca gtagctgtaa
     3001 taataggcgg tgcattcggg aaaatcgttt catcattcgt taatgatatc ttaatgccta
     3061 taataggctt ggctctgggt aaggtaagtt ttaacgattt gaagtgggtt ttggcaactg
     3121 attctgaggg aaatataact tcatcagtaa actacggaac atttattcag tttataattg
     3181 attttctaat tatagctttg agcgtgttcc tgataattag attaattgct gttgccaaac
     3241 agaaagctga agaagcaaaa cgcaaaaaag aagctctaga aaaagctgaa gaagcaccac
     3301 cagtaccacc agctccacca gtacctacta aagaagaagt attacttaca gaaataagag
     3361 atttattgaa agaaaagaag taaagtagta tagttatctt gttacaagga ggtcaaaatg
     3421 gacgggtttg atgatttcag caaaaaatta gaaaatgctg gaaagaagtt atttaagaaa
     3481 accaaagagt ttgctgatac tacaaaatta tctataaaac tcaaagattt agaaattgat
     3541 cttgaaaatc tttttcaaca agcaggcaag aaagctttca aaggcgaaga tacaaaagag
     3601 gtgtttgatg agattaccaa tttgcaagga cagatagaat ctctaaaagc agagattgct
     3661 gatgcaaaag atatgaaaat ttgccctaac tgcaaatctg aaatgccaaa agaagctgtt
     3721 gcctgtatga agtgcggaca taagttcgag tagtttatga aaagaataga ttatatttcc
     3781 tgggatgagt atttcatggg tattgctatc ttgtcctcaa aaagaagcaa ggatcctaat
     3841 acctgcgttg gagcatgtat tgttgatgag tttaataaag ttctgtcgtt aggatacaac
     3901 ggaatgccta taggttgttc tgatgatgtt tttccatggg aaagagaagg acaggcttta
     3961 gacacaaaat atccatatgt atgtcatgca gaattaaatg ctattttgaa ttatgatggt
     4021 tacggattaa agggaagcac actttatgta acactgtttc cttgtaatga ttgtgcaaaa
     4081 gctattatac aaaaagggat taagaaggtt gtttatcttg atgataagta ttctgatcaa
     4141 gatactacaa aagcatcaaa gcttatgttt gatgctgcga aagtaagtta tgttaaatat
     4201 gaattaagag gtaaggatat tactataaat ctctaggttt tataacattt ataaggccag
     4261 gaacaacttc catccttaca ctttttcttt ccactatctc accttcaata ttaactttaa
     4321 gcatatcctc gctttctaag gatatgcttt tcccttttgg aaatgttata ccgtctatac
     4381 ctgcatgttt tcccttcatg tatttaggta acactgaaaa tacaaatgcc agatttttgt
     4441 cttcaactat gcacatatca attattccat cactaaaaga agtatgtggg acaggtatga
     4501 aaccaccccc ataatatttc ccatttgtac atgctgcaag cattacataa ccgttatgaa
     4561 taacctgacc gtcaattgta attttaactt tgaatttggt ctttttttga ttcagagctt
     4621 taaaaaagcc caatagatat gcagggacac ctgttatccc atactttctt gtatagtatg
     4681 tggagttata tgcagcttcg gcatctaccc ctaaagatag tatatttata cagtaattga
     4741 atttttcctc atcaaaaagc tttattgcat caatttttat tacttcacct tcaatggtgc
     4801 gtctaactat atcagtttta tttgagtaat ccatcaagca tttaataaaa tcatttccac
     4861 tgcctgcagg aagtatgcct atagggcagt catatcctgc aacaccattt gcagcttcat
     4921 ttattgaacc gtctccacct gctatgtata ttctgtatcc accttttttt gcgtattctt
     4981 cacatatttc ctttgcatgc atagcatact ttgtgtatac aatactgtat tcctgctcac
     5041 tgcttttaaa atatttctct atttccggta cgtaagcggt tgcttttttg tttccggaca
     5101 tgggattgat tataaacaga tgcttcatta gaccccctga tttatcgtat actaatatac
     5161 tgattattta atataatacc acaatatgtg gtgttattga atatcatact gttaaaaggc
     5221 aattaaatat attacaatat aagttgataa tattttaagg agtttttaaa tgaaaaatct
     5281 gcatttagtt tgtaacgctc atatcgaccc agtatggtta tgggaacttg aagaaggagt
     5341 agcagagact ctttcaacat tcagagtaag cgctcaattc tgtgaagagt atgatggatt
     5401 tattttcaac cacaatgaag cacttctata tcaatgggtg gaaaaatacg agccatcact
     5461 ttttaaacgt atacagaaat tggttaaaga taagaagtgg catataatgg gcggatggtt
     5521 tattcaaccg gatggcaata tgccttcagg agagagtttt gtaagacaga tactggaagg
     5581 tcgcaactac tttatggaga aatttggcac attcccgaca acatgtataa attttgatgc
     5641 attcgggcat acaagaggat tagttgatat tttaaaaaac agcggatatg attcttatct
     5701 gttctgtcgt cctgagcctg atatgattga acttcctgat gaggactttt tatgggaagg
     5761 cttcagcggt gatacaatta tgtgcaacag agcatataac tcatatgaat cacatagagg
     5821 ggaagcagac cagaagataa aaggctggat ggaaaataac agaaacagac cagtaggaat
     5881 ggttttatgg ggaattggaa atcatggcgg tggtccttca agagtagatt ttgataacat
     5941 ttctaaaatg gcaaaagaag aaaaagaatt taatataatt cattccacac cggaagcata
     6001 ttttgaacag atgaaagaaa aacaactacc tatttacaaa ggaatcttga atccaaggta
     6061 tataggatgt tatacatctc agatcaggat taagcaactt cataggaaat tagagaatga
     6121 actgtacatg acagaaaaga tggctgcaca tgctgcatct tccaaactca tgaaatatcc
     6181 tcaagataat atacagcaag ctacaaaaga tctattgctt atggaatttc acgatattct
     6241 tcccgggtcg gcaatacaac ctgttgagaa ctattcaata tcattagcag gacacggact
     6301 tgaagaactt ggtagagtaa aaacacaggc atttttgtct ttatgttcag gacagaaagt
     6361 agcaaacgat ggggagattc ctctattggt ttacaatcct catccatatg aaattccata
     6421 tattgttgaa tgtgaatttc aacttgctga tcagaataag aataagaaga tatttgctaa
     6481 tcctgtagta tatcaaaacg gtagaaaact accatgtcag gtggagaagg aagcatcaaa
     6541 cttcaatgtg gactggagaa agaaatcagt attttatgca aagcttaaac caggacagat
     6601 aaacagattc gatgctagaa tagagatgat agattctaag ccagttcctg ttatcagaca
     6661 gacaaaagat aagtttattt ttaaaacaaa ggaaattaca gttcatatat ccaaacttaa
     6721 cggaactatt gataaatatg aagtaaatgg tgttgattac attgctaaaa acgcttttct
     6781 tccggtagtc ataaaggatg atgaaaactc atgggcgcat aaagaaaaga gtttcagaaa
     6841 tactgaaggt tatttcaaag taatggatat aaatgaagga aatgtttttt cagggattat
     6901 tgatgaaaaa cagattgaaa gtgttagagc aatagaagac ggttctgtaa gaactgtaat
     6961 agaagccgtt atgaaatatg ataattcatt tatatgctta aggtatcatc ttcctaaaaa
     7021 aggcactcag ataaaagtaa ctgtaaaagt atattggaat gaaaaaatga aaatgttgaa
     7081 actttctgta ccaacagtaa ttgataatcc ttcatataca ggtcagcaag catatggttc
     7141 tgaaaaactt ttaacaaatg gagatgagat ggtcagccat aaatggaatg ttctttataa
     7201 cagacaaaaa gctctgagta taataaatac aggcacatat ggttctgatg tattggacaa
     7261 cgaaatgagg atttcacttt taagatctgc tggatattca gcaggcaaat cagatttctc
     7321 agtaaggaat aaatatataa tgcctcagga cagattctct ccttatatgg accagggaga
     7381 aagggatttc gagttttatc ttgatgcttc tgacacaaaa acaaggctag aacaaataga
     7441 aagagaagca gctgtatgta atgaaaaacc gatggcgctt tcatttttcc catcaggtca
     7501 gggaaagaaa atcaagccat ttgcaatact ttctgatgat gtaattacta tgccggtatt
     7561 taagagaagt gaagataaga aaaactacat aatacgctta ttcaatccta ctgataaaaa
     7621 aagagtaaca actcttaagt taccaatgct tagatgggaa aatgaatttg aattggatgc
     7681 atatcagctt aagtcatata aactggatgt ttcaacaaag agagtagtag aaacagatct
     7741 tgtagaaagg aataagtagc gaaaatatat aatgttttta gataacaggt tgtatcatat
     7801 cgaaaagtta ttaaggaggt atcacatttt atgcataagc agaaatcttc caagcgagtg
     7861 atatctttgt taacttgtga attttttgat aagagactgg aaaatgaata taggcaatat
     7921 gatatcagta atgccattaa gtatgtcgga cctattttgc ttatgctggg gatttccttc
     7981 tttcttttta taattccaga ctttttcata attcaagata aaaaaacttt acttgttatt
     8041 tttcttaaca gattagtatt tcttgcttta attactcttt tctattttaa aattaaaact
     8101 gctaaaactt atgagtttta ttaccgatgg attacatttt atgaagtgat tgcctgcata
     8161 tttttcatgg ttactttata taattatgaa aatccgaatc tttttataca atcatttgga
     8221 at
//
LOCUS       DAOSXM010000090         8044 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2111
            NODE_5776_length_8044_cov_10.145074, whole genome shotgun sequence.
ACCESSION   DAOSXM010000090 DAOSXM010000000
VERSION     DAOSXM010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864178
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 8044)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8044)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:24:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,261
            CDSs (total)                      :: 2,229
            Genes (coding)                    :: 2,218
            CDSs (with protein)               :: 2,218
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 3 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8044
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5776_length_8044_cov_10.145074"
                     /isolate="AMR_MDS_2111"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            141..341
                     /gene="rpmE"
                     /locus_tag="PLQ68_09375"
     CDS             141..341
                     /gene="rpmE"
                     /locus_tag="PLQ68_09375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016423669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HXK72904.1"
                     /translation="MKKDTHPKYYKTTATCACGNVFEIGSTKKDIKVEICSNCHPYFT
                     GKQKLVDSGGRVDKFKKKYGIS"
     gene            390..1382
                     /locus_tag="PLQ68_09380"
     CDS             390..1382
                     /locus_tag="PLQ68_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HXK72905.1"
                     /translation="MRKTSIGGSALLEGIMMIGPHKKAIAVRKPDGEVELLVENLPKK
                     RKLAKIPIIRGAYSLIYQMVLSIRALTYAASFYDLEEDEKNIPDKSKKADVITEEFSD
                     LVTEQTENSPLEDKSKKNKEKDTETLSKTAVFFSILLSVGITVGLFILLPNFIIGLLK
                     IQNTAVNNLLEGVLRISIFIGYIILVTLMKDIRRVWMYHGAEHKTINCYEAGEELTVE
                     NVRKYTTSNRRCGTSFLFLVLVVSIVVFSFVGWHSPLINMLIRILLLPLVAGLAYELV
                     KLAGRYDNLFSRVISAPGLLFQKLTAKEPDDGMIECAIMAFNAVLPQNEEEAKW"
     gene            1376..>1557
                     /locus_tag="PLQ68_09385"
     CDS             1376..>1557
                     /locus_tag="PLQ68_09385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72906.1"
                     /translation="MVISQLINSYSGFFPDKKEMYLFLEEIFKKDISYFYAHQDQIVE
                     EDKAKLLLEYAKRRIK"
     assembly_gap    1558..1657
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1658..2002
                     /locus_tag="PLQ68_09390"
     CDS             <1658..2002
                     /locus_tag="PLQ68_09390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72907.1"
                     /translation="KVVKSNLFSDIQEKFDIIVSNPPYIPYHEYIYLDKQVADYEPEL
                     ALLAGEEGTEFYEIISKEAKNYLNKDGWVMFEVGYNQSKRVEDILKKQGYSDITINAD
                     IQGIQRVVRAKL"
     gene            2027..2803
                     /gene="mazG"
                     /locus_tag="PLQ68_09395"
     CDS             2027..2803
                     /gene="mazG"
                     /locus_tag="PLQ68_09395"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015357978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HXK72908.1"
                     /translation="MKEKYVFNDLLTIMEKLRSDDGCPWDKEQTHDSLTKCLIEEAYE
                     TCDAISEKSPEKMAEELGDVLLQVVFHAQIGKEEKTFSMDDIIDGICKKLVYRHPHIF
                     GDKKFNSSDQVLESWEKLKIQEKGYSSEADSMNKIPKNFPALMRAYKVQQKAQRVGFD
                     WPEISGAFNKIYEETQELKQAIDTNDSDMQKEEAGDLLFAVVNVCRFLDIDPEQCLNK
                     SSDKFIKRFTYVENSCIDNGLDMKQMTIDELDKYWDEAKK"
     gene            2800..3042
                     /locus_tag="PLQ68_09400"
     CDS             2800..3042
                     /locus_tag="PLQ68_09400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009222599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HXK72909.1"
                     /translation="MRIDKFLKVSRLIKRRTVANQACEGQKIFVNEKAVKPSYNVKKG
                     DILEIKFSNNSIKVKIADIAESVKKQDAASLYELMD"
     gene            3232..3774
                     /gene="infC"
                     /locus_tag="PLQ68_09405"
     CDS             3232..3774
                     /gene="infC"
                     /locus_tag="PLQ68_09405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003520198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HXK72910.1"
                     /translation="MEVNTIAAHKSNKVQYRINENITSPEIRLIGADGNAIGVMPVAE
                     ALKISENAGLDLVEISPNANPPVCKIIDYGKFMFEVTKREKEARKKQKTIELKEIWLK
                     MGIDEHDINFKIKNACKFLADGDKVKVSLTFRGREMTRTQMGGEVLDKFVQACSEYGT
                     PEKSPKLEGRHMIVILAPKK"
     gene            3791..3988
                     /gene="rpmI"
                     /locus_tag="PLQ68_09410"
     CDS             3791..3988
                     /gene="rpmI"
                     /locus_tag="PLQ68_09410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015924814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HXK72911.1"
                     /translation="MPKLKTHSASKKRFSVTGSGKIKMGHAFRRHRLISKTKRAKVDN
                     RSTAYASDADASNIKKLIPYK"
     gene            4003..4347
                     /gene="rplT"
                     /locus_tag="PLQ68_09415"
     CDS             4003..4347
                     /gene="rplT"
                     /locus_tag="PLQ68_09415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HXK72912.1"
                     /translation="MARVKGGVTTRKRHKKILKMAKGYFGAKSKNFRIAKQQVMKSLS
                     YAYTARKANKRNFRQLWIARINAAARLNGMSYSKFISGLRKNGIDLNRKVLADMAVND
                     PQGFASLVSSVK"
     gene            4354..5121
                     /locus_tag="PLQ68_09420"
     CDS             4354..5121
                     /locus_tag="PLQ68_09420"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HXK72913.1"
                     /translation="MNRIESKDNQKIKRLKSLDKRKVRENENLFFIEGKKLIDEAFKS
                     GITITEIYVSDTYYQNNMSYVDKLKCKDIYLIRDGILDSLSYMSSPEGILGLGRPMKW
                     DIFDVIKKGLPVIIFDRINDPKNAGTMVRSAEAFGIGCVFALEGCTDLYNEKAIRASM
                     GSLFRMPYIKISDDVFNILRKAEYDIYGLDANGENIESAKLHKKCAIIVGNESTGLDK
                     FIKKHLTKILSIPMQGQVESLNAACAAGIVMYKISLL"
     gene            5194..5931
                     /locus_tag="PLQ68_09425"
     CDS             5194..5931
                     /locus_tag="PLQ68_09425"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HXK72914.1"
                     /translation="MPKAALQLYSIKEIAEQDFYHAIHIAKQVGYDGVEFAGFYGKEA
                     IETKKYLDDLDILPAGSHVGLSAVVDKFSETMEYNATISNKYIVVPYISEQFHNEDGI
                     KQIAEYLNIASDNAKKYGIKLGYHNHAFEFSIVENGKRLFDILAENTQEDIVLEIDMF
                     WAVKGGVDPVEYCKKYSNRLELLHFKDITSDGFDTEVGKGIVDFPAILKVSDKIDWCV
                     MEQEKFDMPMEKSIAISCEYMKGILNA"
     gene            5924..6533
                     /gene="hisIE"
                     /locus_tag="PLQ68_09430"
                     /pseudo
     CDS             5924..6533
                     /gene="hisIE"
                     /locus_tag="PLQ68_09430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514582.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
     gene            6535..7869
                     /locus_tag="PLQ68_09435"
     CDS             6535..7869
                     /locus_tag="PLQ68_09435"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007491848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HXK72915.1"
                     /translation="MENSNNYKNKRKKPFLVKDKFKNKRKFERRAGHPLKDFSKFKKR
                     PVELDNQKPQASYSGTKFDELSLSEGIMKALEKKNYIHSSTVQADVIPPLMEYRDVIA
                     KAPTGTGKTFAFGIPLIEQMDYDNKDIQALILAPTRELVIQIRDELTDLSAFMPKVRI
                     EAIYGGKAIDVQIKALKRSPQIIVATPGRLKDHIERKTIDISNVRTAVLDEADRMLDM
                     GFIEDVTSILNLMKNRKNLALLSATMSTGVMDISWLYQRDPVEITVEEDNENKPDIDQ
                     LSIMLHNTHAKIKAVADIMDNNDFYKVLVFCNTINMVRMLTDDLYDKGYAVSCIYGGK
                     SQAGREKTIRAFKEEKLNMLVATDVAARGLDITDVDAVINFDLPNDKEQYIHRIGRTG
                     RAMKNGIAISLITPYDIERLEDIVRYNKADIKEFKNDNILKFIQEGVEEDDN"
     gene            7859..>8044
                     /locus_tag="PLQ68_09440"
     CDS             7859..>8044
                     /locus_tag="PLQ68_09440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72916.1"
                     /translation="MIIDAHNHADWHKTDLKGFLKNMDEFNIDVAWLLSWECPRHEYE
                     NYNGGLATYLTKGPIPFE"
BASE COUNT         3052 a         1044 c         1610 g         2238 t
ORIGIN      
        1 aagactaaaa tccgtttccg tgttttaata ctggaattta agttttcact ttacgatatc
       61 attaataaat taatttctta ttgaaatgat tattgcagtg tggtataatc acgcttgcgt
      121 caaattagga ggtgattagg atgaaaaaag atacacatcc aaagtactat aagactacag
      181 cgacctgtgc atgcgggaac gtgtttgaaa taggttcaac aaaaaaagac attaaagtgg
      241 aaatttgttc aaactgccat ccatatttta caggcaagca gaaacttgta gattcaggtg
      301 gaagagtaga taagttcaaa aagaaatatg gaatttctta attgagagaa aggcgggttg
      361 tataatatca acccgatttt tttataccca tgagaaaaac atcaatagga ggctctgcct
      421 tattagaagg tataatgatg atagggcccc ataaaaaagc aatagctgta agaaaaccag
      481 atggagaagt ggaacttctt gtcgagaatc ttcctaaaaa aagaaaactt gcaaaaattc
      541 ctattattcg tggtgcatat tctttgatat atcagatggt gttaagtata cgagctctta
      601 cttatgctgc atctttttac gatcttgagg aagatgaaaa aaatatacca gataaatcaa
      661 aaaaagctga tgtaatcaca gaggaatttt cagatttagt gacagagcag actgaaaata
      721 gccctttgga agataaaagt aaaaaaaata aggaaaaaga cactgaaaca ttaagcaaga
      781 cagcagtttt tttctcgatt ttgttatctg ttggtattac agtaggcctg tttatacttc
      841 taccaaactt cattatagga ttattaaaaa tacagaatac agccgtaaat aatttactag
      901 aaggcgtttt aagaatttca attttcattg gatacattat tttagttact cttatgaagg
      961 acataaggag agtatggatg tatcacggtg ctgaacataa gactataaac tgctatgaag
     1021 caggtgagga acttactgta gaaaatgtca gaaaatatac taccagtaac agaagatgcg
     1081 gaactagttt cttatttctg gttttggtgg tttctatagt tgttttttca tttgtaggat
     1141 ggcacagtcc attaataaat atgcttatcc gtatactgct gcttccatta gttgcaggtt
     1201 tggcatacga actagtaaaa cttgcgggaa gatatgataa cctgttttca agagttatat
     1261 cagctccagg actgcttttt caaaagctta cagcaaaaga gccagatgat ggaatgatag
     1321 agtgtgctat aatggctttt aatgcagtac tcccacaaaa tgaagaagag gcgaaatggt
     1381 aatatctcag ttaataaact catattcagg ttttttccct gataaaaagg agatgtattt
     1441 atttcttgag gagattttca aaaaagatat atcatatttt tatgctcatc aagatcaaat
     1501 tgttgaagaa gataaagcta aactgttact agaatatgct aaacgtcgca taaaaaannn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnaag gttgtcaaga gcaatctgtt
     1681 tagtgatata caagaaaagt ttgacataat agtttcaaat cctccatata taccttatca
     1741 cgagtatata tatttagata agcaggttgc agactatgag cctgaacttg ccttattagc
     1801 aggtgaagag ggtacagaat tttatgaaat catttctaaa gaagctaaaa actatcttaa
     1861 taaagatgga tgggtaatgt ttgaagtggg gtataatcaa tcgaaaagag ttgaagatat
     1921 actaaaaaaa caaggttatt cagatataac tataaatgcg gatatccaag ggattcaaag
     1981 ggttgttcga gctaaattgt aagtgtaaaa tgtaataagg aatattatga aagaaaaata
     2041 tgtatttaat gatctactaa caataatgga aaaattacgg tctgatgatg gatgtccatg
     2101 ggataaagag cagacccatg actcattgac gaaatgtctc attgaagaag catatgaaac
     2161 atgtgatgca atttccgaaa aaagccctga aaaaatggct gaagaattag gagatgtatt
     2221 acttcaggtg gtatttcatg cgcaaatagg taaagaagaa aaaacatttt ccatggacga
     2281 tattattgat gggatatgta aaaagcttgt ttacagacac ccacacatat tcggagataa
     2341 aaagtttaat tcatctgacc aggtattaga aagctgggag aaattaaaaa tacaggaaaa
     2401 gggttactct agtgaagcag acagcatgaa taagatacct aaaaattttc ctgcattaat
     2461 gagagcatat aaggtacagc aaaaagcaca gagagtaggt tttgactggc cagaaatatc
     2521 aggagctttc aataagatat atgaagaaac tcaggaactc aagcaggcta ttgacactaa
     2581 tgattctgat atgcagaagg aagaggcagg agatttactc tttgctgtag ttaatgtatg
     2641 cagattttta gatattgatc ctgaacagtg cttaaataaa tcatcagata aattcataaa
     2701 aagatttaca tatgtggaaa atagctgtat agataatggt cttgacatga agcagatgac
     2761 tatagatgag ctagataaat actgggatga ggcaaaaaaa tgagaataga taaatttctt
     2821 aaagttagca ggctgattaa gagaagaact gttgcaaatc aagcctgtga gggacagaag
     2881 atttttgtaa acgaaaaagc ggttaaacca tcatacaacg ttaaaaaagg agatatactt
     2941 gaaattaaat tctccaataa ttccattaaa gtaaaaatag ctgatattgc agaatctgtt
     3001 aaaaagcagg atgcagcatc tttgtacgag ttaatggatt aaataaatat atatgagtgt
     3061 aatttgggta aaataatatt tgacattgct atactcatat gataatattc caaaaggaaa
     3121 gcagaagtaa tcccttctca cccctacggc ttatgcactg aacgggtaaa catatcgcat
     3181 tgattgttat atggtcttgt ggattcgctt ctgcaagacc attaaaacta tatggaggta
     3241 aatacaatag cagctcataa gagtaacaaa gttcaataca gaattaacga aaacataact
     3301 tcgcccgaaa taaggctgat cggggcagac ggaaacgcga tcggtgtaat gccagttgct
     3361 gaagccttaa aaattagcga aaatgcagga ttggatttag tggaaatttc accaaatgca
     3421 aatccgccag tctgcaagat aattgattac ggaaaattta tgtttgaagt caccaaaaga
     3481 gaaaaagaag ccagaaagaa acagaaaact atcgagttaa aagaaatctg gctgaaaatg
     3541 gggattgatg agcatgatat taactttaaa atcaagaatg catgcaagtt tttagcagat
     3601 ggagataaag tcaaggtaag tcttacattc agaggaagag aaatgacaag aacgcaaatg
     3661 ggaggagaag tcctcgataa atttgttcaa gcatgctctg aatatgggac accggaaaaa
     3721 tcaccaaaac ttgaaggcag acatatgata gtaatattag caccaaagaa ataaatagga
     3781 ggatttaaat atgccgaagt taaagacaca cagcgcttca aaaaagaggt tctcagtaac
     3841 cggaagcgga aaaataaaga tgggacatgc tttcagaaga catagactga tatctaagac
     3901 aaaaagagca aaggtagata acagaagtac tgcttatgca tctgatgctg atgcatcaaa
     3961 tattaaaaag cttatcccat ataaataagg aggaatatag aaatggcaag agttaaaggc
     4021 ggtgtaacaa cccgcaaaag acataaaaaa atattaaaaa tggctaaagg ctattttgga
     4081 gctaaaagca aaaattttag aatagctaaa caacaggtaa tgaagtcgtt aagctatgca
     4141 tatacagcaa gaaaagcaaa caaacgtaat ttcaggcagc tttggatagc aagaattaat
     4201 gcagctgcaa gacttaacgg aatgagttat tcgaaattta tttctggtct cagaaagaac
     4261 ggtatcgatc ttaacagaaa agtacttgca gatatggctg ttaatgatcc tcagggcttt
     4321 gcttcacttg tcagttcagt aaagtaaata ttaatgaacc gaatagagtc caaggacaat
     4381 caaaaaatca aaagattaaa aagcttagat aagagaaagg ttcgcgagaa tgagaacctt
     4441 ttttttatcg aaggtaaaaa attaattgac gaggctttta aaagcggtat tacaataaca
     4501 gaaatttatg tttctgatac atattaccag aacaatatgt cctatgtcga taagttaaag
     4561 tgtaaggata tctaccttat aagagatgga atacttgatt cgctttctta tatgtcaagt
     4621 ccagaaggaa ttctaggcct aggtcgtcca atgaagtggg atatatttga tgtcattaag
     4681 aagggtttgc ctgttattat ttttgacagg attaatgatc ctaaaaatgc tggtacaatg
     4741 gtaagaagtg cagaagcatt cggaataggt tgtgtatttg cccttgaagg ttgtacagat
     4801 ttatataatg aaaaagcaat aagagctagt atgggttcat tatttaggat gccatatata
     4861 aaaatatcag atgatgtatt caacatttta agaaaagctg aatacgatat ttatggttta
     4921 gatgctaatg gtgaaaacat tgaatcagca aaattacata agaagtgtgc aataattgta
     4981 ggaaatgaat caaccggatt ggataaattt attaaaaaac atctgacaaa gattttatca
     5041 atacctatgc aaggccaagt agaatcatta aacgcggcat gtgcagcagg aatcgttatg
     5101 tataagatat ctcttttgtg attttaaatt aataataaat agtatataat gaaatagcta
     5161 tggaataata gctataggat aatggaggga ataatgccaa aagcagcgtt acagctatat
     5221 tcaataaaag aaattgcaga gcaggatttt tatcatgcta tacatattgc taaacaagtc
     5281 ggatatgatg gagtggaatt tgcaggattt tatggaaaag aagcaataga aactaaaaaa
     5341 tatcttgatg atctagatat attgcctgca ggaagccatg taggtttgag tgcagttgtt
     5401 gataagtttt cagaaacaat ggagtataat gccacaataa gtaataagta tatagtagtt
     5461 ccatatattt ctgaacaatt ccataatgag gatggaatca aacagatagc cgaatacctt
     5521 aatatagcaa gtgataatgc taaaaaatat ggaataaaat tggggtatca caatcatgct
     5581 tttgaatttt ccattgttga aaatgggaaa agactatttg atatattagc ggaaaatacg
     5641 caagaggata tagtacttga gatagacatg ttttgggcag taaaaggtgg agtagatcct
     5701 gttgagtatt gtaaaaagta cagtaacaga ctagaattac tgcactttaa ggatatcaca
     5761 agcgatggat ttgataccga agtcgggaaa ggtatagttg attttcctgc aattttaaag
     5821 gtatcagata agattgactg gtgtgttatg gaacaggaaa aatttgatat gccaatggaa
     5881 aaaagtatag caatcagctg tgaatatatg aaaggaatac ttaatgcttg atcttaagta
     5941 tgacgaaaaa ggattaatac ctgttatttg ccaagatgcg gatacattgg aagtgctgat
     6001 gatggcatat gcaaaagagg aacagataca aaagactttg cagacaggtt atgctacata
     6061 tttttcacgt tcacgaaaca ttgaatggat aaagggaatg accagtggaa atactcaaaa
     6121 ggttattaat gttctagtag actgtgatct agatacagta atatatcttg ttaagaaaaa
     6181 tggtcctgct tgtcatacag gagcccaatc atgttttttc aggaaaataa ataaagatgg
     6241 ggagatagtt agtaatgaat gaaatattga acgagttata caaggttgtt tatgacagaa
     6301 agaataatcc aaaggaaggt tcatatacct gttatctatt tgataaaggt ttggacaaaa
     6361 tattaaaaaa aataggtgag gaagcctcag agactataat cgctgctaaa aatgattcta
     6421 aagatcaaat catatacgaa gtatctgatc ttctttatca tctagtcgtg ttacttgttg
     6481 agaaggaaat taccctagag cagatagaag aggaattgaa aaatagaagg taaaatggaa
     6541 aattcaaata attacaaaaa taaaaggaaa aagccctttt tagttaaaga caaatttaag
     6601 aataagagaa aatttgaaag aagagcaggt catccgctta aagatttctc aaaattcaag
     6661 aaaagacccg tagaattaga taatcagaaa ccacaagcaa gttattcagg tacaaaattt
     6721 gatgagctta gtctttctga aggtataatg aaagcacttg agaaaaagaa ctatattcat
     6781 tcatcaactg tacaagctga tgtaataccg cctttgatgg aatataggga tgttatagcg
     6841 aaagctccta caggaactgg aaaaacattt gcatttggaa taccgctaat agagcagatg
     6901 gattatgaca acaaagatat acaggcatta atacttgcac caacaagaga gcttgttatt
     6961 cagattaggg atgagttaac ggacttatct gcttttatgc caaaagttag aattgaagct
     7021 atatatggcg gcaaagcaat agatgtccag ataaaagcat taaaaaggag tccgcagatt
     7081 attgttgcta caccaggtcg tcttaaagat cacatagaaa gaaaaacaat agatatttca
     7141 aacgtaagaa ccgcagtact cgatgaggca gacaggatgc tggatatggg ctttattgaa
     7201 gatgtaacaa gtattttgaa tttaatgaag aatagaaaaa atcttgcact tttatctgca
     7261 acaatgtcaa ctggtgttat ggatatatct tggttatatc aaagagatcc tgttgagatt
     7321 actgtagagg aagacaatga aaataaacca gatattgacc agttaagtat tatgcttcac
     7381 aatactcatg caaaaattaa agctgttgct gacataatgg ataacaatga tttttataaa
     7441 gtccttgttt tttgtaatac tattaatatg gttagaatgc tgacagatga tttatatgat
     7501 aaaggatatg cagttagctg tatatacggc ggaaagtcgc aagcaggaag agaaaagact
     7561 atcagagcat ttaaagaaga aaaactcaat atgcttgtag ctactgatgt tgcagcaaga
     7621 ggtcttgata ttactgatgt tgatgcagtt ataaattttg atcttcctaa tgataaagag
     7681 caatatattc atagaatcgg aagaacagga agagcgatga aaaatggtat agcgatatct
     7741 ttaataacac cttatgatat tgaaaggcta gaagatatag ttagatataa caaagcggat
     7801 ataaaagagt ttaagaacga taatatatta aaatttattc aagaaggagt agaagaagat
     7861 gataattgat gcccataatc atgccgactg gcataaaacc gatttaaaag gttttttgaa
     7921 aaatatggat gagtttaata ttgatgttgc ctggcttcta tcatgggagt gtcccagaca
     7981 tgaatatgag aactataacg ggggattggc tacttatctt acaaaaggtc caataccatt
     8041 tgaa
//
LOCUS       DAOSXM010000091         8013 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2111
            NODE_5794_length_8013_cov_9.901860, whole genome shotgun sequence.
ACCESSION   DAOSXM010000091 DAOSXM010000000
VERSION     DAOSXM010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864178
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 8013)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8013)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:24:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,261
            CDSs (total)                      :: 2,229
            Genes (coding)                    :: 2,218
            CDSs (with protein)               :: 2,218
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 3 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8013
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5794_length_8013_cov_9.901860"
                     /isolate="AMR_MDS_2111"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            103..720
                     /locus_tag="PLQ68_09445"
     CDS             103..720
                     /locus_tag="PLQ68_09445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72917.1"
                     /translation="MSVFERFMMGLIFAAALPIALFNVGWKFCEYFYNGDYLVICALS
                     GLILGIIADILFLRKILLNAYSAGTVLPIFIYAFYLICFLLCFRKFPIFILIPGLFAG
                     IYEGRKLFHFKANSYESGYRIERVSQLSSAGMAISCIISAFFVYLEFDKTVDFIRRLF
                     NTPDLIIEQWIVLISIILASTVLIFIQYWITRKAALLAYGKEERK"
     gene            717..1622
                     /locus_tag="PLQ68_09450"
     CDS             717..1622
                     /locus_tag="PLQ68_09450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015360365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXK72918.1"
                     /translation="MKKIDIHVHVRQYLTKYEEETAILRPSSKDTYITPEQLKVLYDK
                     LGIEKGVLLPGESPEGKHYSVTNQETMRIAKDYPDMFYWFCSVDPRWDTNRPDTDLSY
                     YINYFKSHGAKGIGELTAKLPFDEPRMKNLFYHAEKCDMPVLFHVGTDSSPYGAYDIL
                     GLHKLESVLSEFPKLIFIGHSQPFWAEIGDDVTQTSRNGYPKGPINKEGRVVELMRKY
                     PNLHADLSANSGYNAVSRDPDFGYSFLEEFKDRIYYGTDICANRNYEFFKLADFLKEG
                     KDSNKLSKEAYELICRSNALNLLER"
     gene            1630..2538
                     /locus_tag="PLQ68_09455"
     CDS             1630..2538
                     /locus_tag="PLQ68_09455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72919.1"
                     /translation="MMKIDMHIHGIMFVNDGEEKNSITRPGKDTFATAEYLRIAYKKL
                     GIEKGVLLPIVSPEGQHRIISNEENYRMVCDNPDLFDWFCCIDPRWGNNRDDTDLGYF
                     IEHYKKLGAKGIGEVTCKLPFDNPLVKNMFYHAQNFNMPILFHIGAEREPYGLFDYAG
                     LPLLEKTLEAYPDLKFIGHSASFWAEISGDCTDDNRAGYPKGPVVKGGRVPKLLLDYP
                     NLYADISAGSGYNAITRDKDFGYSFLEEFQDKLFYGTDICAPKEYEFFKLADFLENGL
                     SENKLSKTAYNKICRLNAESFLLSQS"
     gene            2698..3081
                     /locus_tag="PLQ68_09460"
     CDS             2698..3081
                     /locus_tag="PLQ68_09460"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HXK72920.1"
                     /translation="MIITRNTVQREIILQTVLNMDNHPSADDVYERLRNDNPSISRAT
                     VYRNLELLAKQGKILHIRMPNGADCFDFNTSEHYHLRCDICRRVFDIDLPKIPKINIE
                     QKKYKDFTIEGYSISFHGICPECKK"
     gene            3099..3635
                     /locus_tag="PLQ68_09465"
     CDS             3099..3635
                     /locus_tag="PLQ68_09465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015392522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HXK72921.1"
                     /translation="MKDLKGTKTEANLKEAFAGESMARNKYTYFASKAKKDGYVQISK
                     IFEETANNEKEHAKIWFKLLDGISETPDNLISAAEGENYEWTDMYARFAKDAKAEGFK
                     EIAVLFEQVAKIEKEHEQRYRKLLSNINDGLVFSRDSEMIWQCSNCGHIVIGKKAPEM
                     CPVCDHPKAHFELKATNY"
     gene            complement(3821..4891)
                     /locus_tag="PLQ68_09470"
     CDS             complement(3821..4891)
                     /locus_tag="PLQ68_09470"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HXK72922.1"
                     /translation="MKELTSKERFFRMFQHKEADRIPIIDAPWEGTLRRWVKEGMPKD
                     VDWRDYFNIDKVSRITVDTSAQYEVKVIEEDDKQITYTTAYGVTLRKFKQEDSTPEFL
                     DYKVNNSKIWFEETKKRMQEGENRINWERYERVYPDWIKNGHWIEAGFWFGFDVTHSW
                     MCGTETVLIAMLEEPEWVMDMFDTYLNSCIKHFDILWDKGYRFDSIFWYDDMGYKNTT
                     FFSKDTYRELLRPFHKKAVDWAHNKGIYAHLHSCGDVMPFAEDIVEIGIDALNPLEIK
                     AGMDPVFLKKKYGDKLVLHGGINAVLWDNTEAVTQAINEVVPILKEKGGYIFSSDHSI
                     PNSVSLENFKKIVETVKKVGSY"
     gene            4965..5744
                     /locus_tag="PLQ68_09475"
     CDS             4965..5744
                     /locus_tag="PLQ68_09475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010296584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HXK72923.1"
                     /translation="MNKKALYEKWKIEEEKTFSGWDFSNMSGCCSTETPWDYRSIVKS
                     FLRNEMNLLDMGTGGGEFLLTLTHPYEKTWVTESYMPNISLIKKNLIPLGIHLCEVDK
                     DSNLNLSSNFFDIVTNRHESYDLEEIRRILKRDCLFITNQVGGDNNIMLSSRLIDGYK
                     PKYPNHNLNNEKTKFISEGFEIIYSNEAIMPSMFYDVATIVRYAKIIEWEFPNFSVDK
                     CLDRLYELHDEICEKGYVASNESRFIIVAVNRKELKSNDKR"
     gene            5731..6183
                     /locus_tag="PLQ68_09480"
     CDS             5731..6183
                     /locus_tag="PLQ68_09480"
                     /inference="COORDINATES: protein motif:HMM:TIGR00022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HXK72924.1"
                     /translation="MINGNIDGIGYSNIENEFIKKAFEIMKKLNADTQLGRTDIEESD
                     FYYTVMEVDVAPLNEIKHEMHKKYIDIHYLIKGHEAIGIANSKRLGVLVPYSEEKDCA
                     FLKDGNLVSTVVMEPGDFCIIYPYEAHKPGGMFNEFGKIKKAVFKIKV"
     gene            6286..7920
                     /locus_tag="PLQ68_09485"
     CDS             6286..7920
                     /locus_tag="PLQ68_09485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003511658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HXK72925.1"
                     /translation="MSITQILEKNARLYADDVCLVEINPEVQEKKEVTWKEYELIEAN
                     PQEHYRKEITWKEFDDKANRFANFLLANNIKKGNKVAILLMNCIEWLPIYFGILKTGA
                     LVVPLNYRYTADEIKYCIDLSEADAMIFGFEFIGRVESIKDKINHIKPLIYTGKNCPD
                     FAISYEKQIKNYSETAPDIEISDEEDAALYFSSGTTGFPKAILHSHANLLASCITENA
                     HHKQTRQDNFLCIPPLYHTGAKMHWFGSFLVGGKAVLLRGVKPKWILKAVSEEQITIV
                     WLLVPWAQDILDAVESGEISLNDYDMAQWRLMHIGAQPVPPSLIKRWKEYFPGHEYDT
                     NYGLSESIGPGCVHLGIENIHKVGAIGVPGYGWMTKIVDDKGQEVKQGDVGELAVKGP
                     GVMKCYYNDKEATDAVLKDGWLYTGDMAKMDDEGFIYLVDRKKDVIITGGENIYPVQI
                     EDFLRSHRAIKDVAVIGLPDKRVGEIAAAIIELKKGHACTKEDLEAFCEALPRYKRPR
                     VIIFDNIPRNPTGKIEKPKIREKYCKVSVVELQMSN"
BASE COUNT         2849 a         1086 c         1527 g         2551 t
ORIGIN      
        1 aagaaaacta aaatgtggtg aaatataatt tttatatgtt aaaatgtatt taacttacgt
       61 aattgataaa taaaattcaa ttatggaggt tactggataa ccatgagtgt ttttgaaaga
      121 tttatgatgg gactgatatt cgcagctgcg cttcctattg cattattcaa tgtaggatgg
      181 aagttttgcg agtattttta taatggggat tatctggtaa tatgtgcttt atccggtttg
      241 attctgggaa taatagctga tattttattt ctcagaaaaa ttcttttaaa tgcatatagt
      301 gcaggtactg tattaccaat atttatctat gcattctacc taatatgttt cttattgtgt
      361 tttaggaaat ttccaatatt tatcctgatt ccaggattgt ttgctggtat ttatgaagga
      421 agaaaattat ttcattttaa agctaattca tatgagtcag gatatagaat tgaaagagta
      481 tcgcaacttt catctgctgg aatggcaata tcttgtatta taagtgcctt ttttgtatat
      541 ttggaatttg ataaaactgt agattttata agaagattat ttaatactcc agatttgatt
      601 attgagcagt ggattgttct tatatctatt atattagcaa gtacagtact catattcatt
      661 caatactgga ttacaaggaa agcagcatta ttagcatatg gaaaggaaga gagaaaatga
      721 aaaaaattga catccatgtt catgttagac agtatcttac caaatacgag gaagaaacag
      781 ccattttgcg gccatcttca aaagatacat atattacacc agaacagtta aaggtcttgt
      841 atgacaaatt aggaatagaa aaaggagtat tacttcctgg ggagtctcct gaaggaaaac
      901 attattcagt aactaatcag gagacaatga gaattgcaaa agattatcca gatatgtttt
      961 actggttttg cagtgtagat ccaagatggg atactaacag accagatact gatttaagct
     1021 actatattaa ttatttcaaa tcgcatggag caaagggtat tggagaactg acagcaaaac
     1081 ttccattcga tgaacctaga atgaaaaatc tgttttatca cgctgaaaaa tgtgatatgc
     1141 ctgttctttt ccatgtagga actgatagca gcccgtatgg tgcttatgac atattagggt
     1201 tacataaact ggaatcagta ttgtcagaat ttcctaaatt aatatttata ggccattctc
     1261 agcctttctg ggcagaaata ggtgatgatg taactcaaac cagcagaaac ggatatccta
     1321 aaggtccaat aaataaagaa ggcagggtag tagagctcat gaggaaatac ccgaatctgc
     1381 atgctgatct atctgctaac agtgggtata atgctgtttc aagagatcct gatttcggat
     1441 actcatttct agaagagttt aaagacagga tttattatgg tacagatata tgtgcaaata
     1501 gaaattatga atttttcaaa ctagcggatt tcttaaaaga aggaaaagat agtaataaat
     1561 taagtaaaga agcatatgaa ttgatctgca ggtccaatgc tttaaattta cttgaaaggt
     1621 gatattgaaa tgatgaagat tgatatgcat attcatggaa ttatgtttgt taatgatggt
     1681 gaagagaaaa attcgattac cagacctggt aaagacacat ttgctactgc tgaatattta
     1741 aggatagcat acaaaaagct tggtatagaa aaaggagtac tgcttccaat agtttcaccg
     1801 gaagggcagc atagaattat atctaatgaa gaaaattaca ggatggtgtg cgataatcct
     1861 gatctgttcg attggttttg ttgtatagac ccaaggtggg gaaataacag agatgatacg
     1921 gatcttggat actttattga gcattacaag aaattaggtg caaaaggtat aggtgaggtt
     1981 acatgtaaat taccttttga taacccatta gttaaaaata tgttttatca tgctcaaaac
     2041 tttaatatgc ctattctatt tcatatagga gcagaaagag agccatatgg cttatttgat
     2101 tatgcaggtt taccacttct tgaaaagaca ttggaagcat atcctgattt aaaattcatt
     2161 ggtcactcag cctccttctg ggcagagata agtggtgact gtacagatga taatcgagca
     2221 ggttatccaa aaggacctgt tgtaaaaggt ggcagagtac cgaagcttct tttggattac
     2281 ccaaatctgt atgctgatat atcagcggga agcgggtata atgcaatcac aagggataag
     2341 gatttcggat atagctttct tgaagagttt caggacaaat tattttatgg aacagatatt
     2401 tgcgcaccta aggaatatga gttttttaag ttagcagatt ttcttgaaaa cggtttgagt
     2461 gaaaacaagt taagtaaaac tgcttacaat aaaatatgca gattaaatgc agaaagtttt
     2521 ttgctttccc aaagttgaat attcaaaaaa tagaaattca tcaaataata tgaaatttaa
     2581 ttaacattta tagttaattg gttgagtttc ttgtattttt ttaataaatt tcactttttg
     2641 atgttgccaa ataaaaaata tcgtgtataa tcttaatgag attggatatt ggtaattatg
     2701 attataacta gaaacacagt acaaagagaa attattctac agaccgtttt gaatatggat
     2761 aatcatcctt cagcagatga tgtttatgaa agactcagga atgacaaccc atctatcagc
     2821 agagcgactg tatacagaaa tcttgaattg ttagcaaaac aagggaaaat actgcatata
     2881 aggatgccaa atggtgcaga ttgttttgat tttaacacat cagagcacta ccatttaaga
     2941 tgcgacatat gcagaagagt atttgatatt gatttaccga aaatacctaa aatcaatatt
     3001 gaacagaaaa agtataaaga ttttacaata gaagggtata gtatatcatt tcatggtatt
     3061 tgcccagaat gtaaaaaata atataaaagg agagttatat gaaagattta aaaggaacta
     3121 aaacagaagc taacttaaaa gaagcatttg ccggcgaatc tatggcaaga aataagtata
     3181 cttatttcgc atcaaaagct aaaaaagacg gttatgtgca gatatcaaaa atatttgaag
     3241 aaactgcaaa taatgaaaaa gaacatgcaa aaatatggtt taaattactt gatggaatta
     3301 gtgaaacacc tgataattta ataagtgctg cagagggtga gaactatgaa tggactgata
     3361 tgtacgcaag atttgcaaaa gatgcaaaag cagaaggttt taaggaaata gctgtattat
     3421 ttgagcaggt agctaaaatt gaaaaagagc atgagcaaag atacagaaaa ctgttaagca
     3481 atataaatga tggcttagtt ttttcaagag atagcgaaat gatttggcag tgcagtaatt
     3541 gcggacatat agtaataggt aagaaagctc ctgaaatgtg ccctgtatgt gatcatccaa
     3601 aagctcattt tgagttaaag gcgacaaatt attaatttgt ttaatgctgg tattaggtgt
     3661 tagaatagta ttttatagaa aataaaataa ttaaaatatt taaaatcaaa aagacggtta
     3721 taccgtcttt tttgaaactt gttagaatta tctttcttgt tagaattttt cttttttatt
     3781 acattgtaaa ttagttataa ttatttataa taatcaaact ttaatagctt cctacctttt
     3841 taactgtttc tacgattttt ttaaagtttt caaggcttac actgttagga attgaatggt
     3901 cagaagagaa aatatatcct cctttttcct taagtattgg gacaacttca ttaatagcct
     3961 gagtaacagc ttcagtatta tcccataata cagcattaat tcctccatga agaacaagtt
     4021 tatctccata tttctttttt agaaagactg gatccatacc tgcttttatt tccaaaggat
     4081 taagagcatc tattcctatt tcaacaatat cctctgcaaa aggcataaca tcaccacaag
     4141 aatgaagatg tgcatatatc cctttgttgt gtgcccaatc aactgctttt ttatgaaaag
     4201 gtcttagaag ctccctgtat gtatcttttg aaaaaaaagt agtatttttg taacccatgt
     4261 catcatacca gaatatggaa tcaaatctat atcccttatc ccaaagtatg tcaaaatgct
     4321 ttatgcaaga attaagataa gtatcgaaca tgtccataac ccactctggt tcttcaagca
     4381 tagctattaa aacggtctca gtaccgcaca tccacgaatg tgtaacgtca aaaccaaacc
     4441 agaatcctgc ttctatccaa tgaccatttt taatccagtc aggataaact ctttcataac
     4501 gttcccaatt tattctgttt tctccttctt gcattctctt ttttgtttct tcaaaccata
     4561 ttttagaatt attgacttta tagtctaaaa attcaggagt tgagtcttct tgtttgaatt
     4621 ttcttaaggt aactccatat gcggtagtat aagtaatctg cttgtcgtct tcttcaataa
     4681 cttttacttc atattgagca gaggtatcaa cggttatcct tgatacttta tcaatattaa
     4741 aataatctct ccaatctaca tcctttggca ttccttcttt tacccatctt cttaatgttc
     4801 cttcccaggg tgcatcgatt atgggtatcc tgtctgcctc tttatgttga aacattctga
     4861 aaaatctttc ttttgatgtt aattctttca ttaaaatctc ctattgccta cttatattat
     4921 gtaacaagta tactataaat aatttcaagt gtataatcta agttatgaac aagaaggcgc
     4981 tttatgagaa atggaaaata gaagaagaga aaacattttc tggatgggat ttttccaata
     5041 tgtctggttg ttgcagcacg gaaacaccat gggattatcg ttcaattgta aaatcttttt
     5101 tgaggaatga aatgaatctt ctggatatgg ggactggcgg aggggagttt ttacttactc
     5161 ttacacatcc atatgaaaag acatgggtaa ccgagagtta catgccgaat atttcattaa
     5221 ttaaaaagaa tcttatacct ttaggtatac atctttgtga agttgataaa gatagcaatt
     5281 taaatcttag cagtaacttt tttgatatag tcaccaacag gcacgaatca tatgatttag
     5341 aggaaataag aagaatatta aaaagagact gtctttttat aacaaatcag gtaggagggg
     5401 acaacaatat tatgctttca tcaagactta ttgacggtta caagccaaag tatccgaatc
     5461 ataatcttaa taatgaaaaa actaagttta tcagtgaggg atttgaaata atttatagta
     5521 atgaagcaat tatgccatct atgttttatg atgttgctac cattgtaaga tatgctaaaa
     5581 ttattgaatg ggagtttcca aacttttcag ttgataaatg cttagacaga ttatatgaac
     5641 ttcatgatga aatatgtgaa aagggatatg ttgcaagtaa tgagtcaagg tttataatag
     5701 ttgcagtaaa taggaaggaa ttaaaaagta atgataaacg gtaatattga tggaataggc
     5761 tatagcaata tagaaaatga atttattaaa aaagcatttg agataatgaa aaagcttaat
     5821 gctgatacac agttgggaag aacagatata gaggaatctg atttttatta tacagtaatg
     5881 gaagttgatg ttgcaccttt gaatgagata aaacatgaaa tgcataagaa atatattgat
     5941 attcattatc tcataaaagg acatgaagct ataggtatag cgaattcaaa aagattagga
     6001 gtattagttc catattctga agaaaaagac tgtgcatttt taaaagatgg gaacctggtg
     6061 agtactgttg taatggagcc tggagacttt tgtattattt atccatatga agctcataaa
     6121 ccaggcggaa tgtttaatga atttggcaag ataaagaaag ctgtttttaa gataaaggtg
     6181 taataaaatg ttgttttatc ttttaaaact gttataatta acaaaatgta atctgtgact
     6241 tttgcagaaa aatgcagcat ggtaggaagg taggataagt aatttatgtc cataactcag
     6301 attttagaga agaatgcaag gctttatgct gatgatgtat gtttagtgga gattaaccca
     6361 gaagtacagg aaaaaaaaga agtaacatgg aaagaatacg agttaataga agctaatcct
     6421 caggaacatt acagaaaaga aataacctgg aaagaatttg atgataaagc aaacagattt
     6481 gcaaatttct tattagcaaa caacataaaa aaaggtaaca aggtagctat tttgttaatg
     6541 aactgtatag aatggcttcc tatatacttt ggaattctta agacaggcgc attggtcgtg
     6601 ccattaaatt ataggtatac tgctgatgaa atcaaatatt gtatagattt gtcagaagca
     6661 gatgcaatga ttttcgggtt tgaattcata gggcgagttg agagtataaa agataagatc
     6721 aatcacataa aaccacttat atacacaggc aaaaactgtc cagattttgc aattagttat
     6781 gaaaagcaga taaagaatta ctctgaaact gctccagata tagaaatatc cgatgaagaa
     6841 gatgcggcat tgtatttttc atcaggtact acaggatttc caaaagctat tcttcattct
     6901 catgcaaacc tattagcttc atgcattact gaaaatgctc atcataaaca gacaagacag
     6961 gataatttct tatgtatacc gcctttatac cacacaggtg caaaaatgca ttggtttgga
     7021 agcttcttag ttggtggaaa agcggttctt cttaggggag taaaacctaa atggatatta
     7081 aaggctgtat cagaagagca gataactata gtctggcttt tagttccatg ggctcaggat
     7141 atacttgacg ctgttgaaag cggtgagata agtttgaatg attatgatat ggcacagtgg
     7201 aggctgatgc atataggagc ccagccagtt cctccaagtc ttataaagcg ctggaaggaa
     7261 tattttccag gtcatgagta tgatacaaat tatggtttaa gtgaatctat cggaccaggt
     7321 tgtgttcatc ttggaattga gaatatacat aaagtcggag caataggtgt tccagggtat
     7381 ggttggatga caaaaatagt tgatgataag ggtcaggaag taaaacaagg ggatgttggc
     7441 gagttagcag ttaaaggacc tggtgttatg aaatgttatt ataacgataa agaggcaaca
     7501 gatgctgttt taaaagatgg ctggctatat accggtgata tggcgaaaat ggatgatgaa
     7561 ggttttatat atcttgttga cagaaaaaaa gatgttataa ttacaggcgg tgaaaacata
     7621 tatcctgtgc agattgaaga tttcttaaga agccataggg cgattaagga tgtagcagtt
     7681 attggtcttc ctgataaacg tgttggtgag attgcagcag ctatcattga acttaaaaaa
     7741 ggacatgctt gtaccaaaga ggatttggag gcattttgtg aagctcttcc aaggtataag
     7801 aggcctagag taataatttt cgacaacata ccgcgaaatc ctacaggtaa aatagaaaag
     7861 ccgaaaatca gagagaaata ctgtaaagta agcgttgttg aattgcagat gagtaattaa
     7921 ctgattaaaa taaaaactta gtgcttttaa ttaaacaaaa cagttgaata atgtatacat
     7981 aatataacaa gatactgtat aattgtaata tat
//
LOCUS       DAOSXM010000092         8000 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2111
            NODE_5805_length_8000_cov_9.217369, whole genome shotgun sequence.
ACCESSION   DAOSXM010000092 DAOSXM010000000
VERSION     DAOSXM010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864178
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 8000)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8000)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:24:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,261
            CDSs (total)                      :: 2,229
            Genes (coding)                    :: 2,218
            CDSs (with protein)               :: 2,218
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 3 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8000
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5805_length_8000_cov_9.217369"
                     /isolate="AMR_MDS_2111"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..339
                     /locus_tag="PLQ68_09490"
     CDS             <1..339
                     /locus_tag="PLQ68_09490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020814574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HXK72926.1"
                     /translation="ERVLINLIHNAIKFTNENGNISIKVEPKKSKVYVYVRDNGIGID
                     QEDLKNIWTRFYKSDKSRGKDRTGTGLGLSIVFSVIKRHNETIDVKSQKGIGTEFVFT
                     LENAEEEENS"
     gene            396..>1129
                     /locus_tag="PLQ68_09495"
     CDS             396..>1129
                     /locus_tag="PLQ68_09495"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HXK72927.1"
                     /translation="MSRKDEQIKFFESDNSDNPKSNGFYMETIKSQKKSFASRVLVPL
                     FVCFLCLVVAASVCVITVVAVMKINSIQTELPFLNLSTQSPAPTASALPTQTQESNNN
                     SASNNQENSETIITYRDVVRIDVLPYEAESTIIEAVEHILPSIAYIRCRQTSEEGTNM
                     SEATALIVSDDGFMITADSAIANLCHENSNELKEGSYIEVCINFDYSNLFFAKVIGRD
                     VVNNIALLKIDTDVILQFIEYGDSDNL"
     assembly_gap    1130..1229
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1230..1626
                     /locus_tag="PLQ68_09500"
     CDS             <1230..1626
                     /locus_tag="PLQ68_09500"
                     /inference="COORDINATES: protein motif:HMM:NF024578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HXK72928.1"
                     /translation="KAIPINKIKQILENLEIETYHNKQMPNLGIGIASEMVVISFDSE
                     QNQEPIHLQGIRIDYIGYGTPAFYSALKKNDIIVSIGEEPIISVQNFFDIKNQYAPGE
                     TIVLTVYRYNEQDEKYMEIPITITQEIAK"
     gene            1626..2255
                     /gene="nth"
                     /locus_tag="PLQ68_09505"
     CDS             1626..2255
                     /gene="nth"
                     /locus_tag="PLQ68_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HXK72929.1"
                     /translation="MKNISSIIKLLKTEYPVVMCTLDFFTPLQLLISTQLAAQCTDER
                     VNLVTPALFNKYKTVYDFAYADIEELESYIRPTGFYHNKAKNIKACCKKLIDEFNEII
                     PDNIDELITLPGVGRKTANVIIAEVFHKPAIIVDTHAKRLSNRIGLSDNTDPDKIEQD
                     LKEILPPEESSNFCHRLVFHGRKVCNARKPKCDLCVINKYCRYFNQKKI"
     gene            2376..3290
                     /locus_tag="PLQ68_09510"
     CDS             2376..3290
                     /locus_tag="PLQ68_09510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007890397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HXK72930.1"
                     /translation="MNSLVKEKNDMKKKNTKGFEKRDKSSTMPFMIPSIAGFLLFFLI
                     PFFIVLFYSFIDNPINKEFVMFDNYISLLRNHAFMLSLKNTLIFSVIAVPLAVIIPLI
                     LALFLESNIPGKSIFRTALISPLVVPVASVILIWQVVFHYNGVINVFLNSIGIDAVDW
                     FKSDFSRLVVVLLFLWKNTGYNMILFMAGLANIPIDVKEAALIDGVGPVRRFFRIKLN
                     YMSSSIFFVTILSLINSFKVFREVYLLTGDYPYEGLYMLQHYINNTFRTLDYQKLSAA
                     AVIICIIITAIIALLFYIDNKLGSSVDE"
     gene            3283..4200
                     /locus_tag="PLQ68_09515"
     CDS             3283..4200
                     /locus_tag="PLQ68_09515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015756138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HXK72931.1"
                     /translation="MSKIGMTKSMKIKRVISISIALVILAFTAAMFIMPIVLTVTNSF
                     MTKSEITANYGKIFSEGGSSAVSSYIADKVNLKIIPDKVSFSQYVTVLVKSPQYLLKF
                     WNSIILVVPIVIFQIIIALGASYSFARYKGRFRNTMFFIYIALMLMPYQVTLVPNYLV
                     ADWMNILETRWSIILPGIFSPFAVFILTKTMQRIPSSIVEAAKIDGANEWKIFRKVFI
                     PMSKNAIYSVAILLFMDYWNMVEQPLIMLQKSEEMHPLSVFLSKINAGEIGLAFAVAA
                     IYMVLPLLLFLYSEDRVLEGVASSSAIKG"
     gene            4213..6231
                     /locus_tag="PLQ68_09520"
     CDS             4213..6231
                     /locus_tag="PLQ68_09520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72932.1"
                     /translation="MKKDASNRRNIVKNIAIVFLAVMLVLTFFSNTILNYSLPEVAVQ
                     YPMYGTISSRIRGQGTVTANQTYQVYFDESRIIEEVYFRRGDIVKKGDILYLLKDAPS
                     DELNNAINTLTTMKINYQRKLMESTPDYTAILEEISQLNKQLIEAKQIKSNLNSNRAL
                     LISIQTEIDLLNKSISELSTKKTNLETYLNSLEPDTSLSEDEFLAALRNASIDYENAK
                     TALDAEKENLKELQRELVKMANPLLVAQANLDAKVLEKSNYIKENPSTDVPTSSEIMS
                     KENDLVTAENASPSDPAEISRLRNELNSMIAKYFEYIPIQERINQYDIEISVLTEELN
                     NIQRQRNEINTNIEISQNRIDVLQFTFEDIEAVYLPLKARYEYEDIEIEIQEVSKTLL
                     ESQDELRILSEEKIEVEGKIMTESEADELIAVLEKSLYDKQQSYEKQKAEDVFDQQLK
                     QIELQNMKREIEVQEALVERLSDKVENNTITAPVSGALLNMRYVAGEKTEAGGVAAEI
                     EISEKGYTMSFSVTVEQARRISVGQQAEIQYYYGSTAPYAQVTGIQTDYNNPQSSRII
                     VLEVIGDNIYPGQTLYVALGETGRDFETVVPNSAVHEDNKGKFVYIVEAKNTPIGNRY
                     IAKRVDIQVIASDDKNSAISGISAYANYVITTASKPLTDGMQVRLVEE"
     gene            6233..7318
                     /locus_tag="PLQ68_09525"
     CDS             6233..7318
                     /locus_tag="PLQ68_09525"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXK72933.1"
                     /translation="MKFKAKLSSKIVVFIIILSVLIVSFMILLNQSNKIVKNRFDQFG
                     AERWMNNDSLFNYAQISCFFTSETGFSENNIYSLSRSYDEKMMSESISSSEGGRLWLH
                     AYSAKGSVSVDASSNIATDVTAVAGDFFFIHNVPLITGSYFKDDPLNNDHILINETLA
                     WQLFGSFDIVGRDIYIYDKPYSISGVTKNDGDVIDDSLPHLYMQYDVYKVLDNLAYIS
                     CYEAILPNPISDFALNVVTSLFSFNELEYEIIQNTSRFNLINTFKNLINLTERNIKTN
                     RVIYPQWENQARITEHKLAYLLLAQIILIFLFICTLTIMIIIYRSKISLFFENNSSKI
                     VTKAKNSKLADKIRNKRRERYEKKKLS"
     gene            7296..>8000
                     /locus_tag="PLQ68_09530"
     CDS             7296..>8000
                     /locus_tag="PLQ68_09530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72934.1"
                     /translation="MKKRNLVKTMVIVGLVIVMLLQLVSCNNKKGAEKTVLDHVFRRT
                     EITLPDNIQSVNNILNVGDRINIIGSGYDQETYENYYVFYSINQDGTDPQEHMLNLNL
                     SKMEGYEGSYMSNIIMLPNEKFLVSIEAWGSDKISGNYKSDNFLVMMDLEGNIDKKVN
                     VRELLEDHLDQDYFYINNIVVDSQSNIYINSDRTVFVLDENFNFSFKVELNENSWLNR
                     LFVYSDGKIAAAINSEG"
BASE COUNT         2928 a         1020 c         1371 g         2581 t
ORIGIN      
        1 gaaagagttc taataaatct tatacacaac gcaataaaat ttacaaacga aaatggaaat
       61 attagcatta aagttgaacc aaaaaaaagt aaagtttatg tttatgtaag agataatgga
      121 attggaatag accaggaaga tttaaagaat atctggacaa ggttttacaa aagtgataaa
      181 tctagaggaa aagacaggac aggaacagga ttaggtcttt caattgtgtt cagtgttatt
      241 aaacgacata atgaaacaat tgatgttaag agtcaaaaag gaattggtac agagtttgtt
      301 tttacactgg aaaatgccga agaagaagaa aattcataaa aagtttacaa tttattttat
      361 taaatgtgat agtatttcaa tgagggtaaa tagatatgtc aagaaaagat gaacagataa
      421 aattttttga atcagataac agtgataatc ctaaatcaaa tggtttctac atggagacta
      481 ttaagagtca aaagaaaagt tttgcttcca gagtattagt ccctcttttt gtatgttttt
      541 tatgccttgt agttgctgca agtgtatgtg ttattactgt tgttgctgtt atgaagataa
      601 attctattca aacagaattg ccgtttctta atttgtcaac tcaatcacca gctccaacag
      661 cttctgcttt gccaacacag acacaagaat ctaataataa ttcggcatct aacaatcaag
      721 agaactctga aacaattatt acttatagag atgtggttag gattgatgtt ttaccttatg
      781 aggcagaatc tacaattatt gaagctgtag agcatatttt gccctcgata gcttatataa
      841 gatgcagaca gacatctgaa gaaggaacaa atatgtcaga ggctacagca ttaattgtaa
      901 gtgacgacgg ttttatgatt acagcagatt cagcaatagc aaatttatgt catgagaaca
      961 gtaatgagct aaaggaaggt tcgtacattg aagtatgtat caattttgat tattctaatc
     1021 tcttctttgc taaggttatc ggaagagatg tagtaaacaa tattgctctg ttaaagattg
     1081 atactgatgt tatacttcaa tttattgaat acggagattc tgataatctn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt aaagctattc ctataaataa aataaagcag
     1261 attcttgaga atctagaaat tgaaacatat cataataaac agatgcctaa tttaggaata
     1321 ggaattgctt cagaaatggt agttatatct tttgactcag aacaaaatca agaacctatt
     1381 catcttcaag gtataagaat agactacatt ggatatggaa cacctgcttt ctattcagca
     1441 cttaaaaaga atgatattat tgtcagcatt ggtgaggaac ctataatatc tgttcaaaat
     1501 ttctttgata ttaaaaatca atacgctccg ggcgaaacaa tagttttaac agtgtacaga
     1561 tataatgagc aggatgaaaa atatatggag attcctatta ctattaccca ggaaattgct
     1621 aaataatgaa aaacattagt tcaattataa aattattgaa aacagagtat cctgttgtaa
     1681 tgtgcacgct ggattttttt acaccattac aactactaat ttctactcaa cttgcagctc
     1741 agtgtacaga tgaaagagta aatttagtta cacctgcatt attcaataaa tataaaactg
     1801 tttatgattt cgcatatgct gatattgaag aacttgaatc atacatcaga ccaaccggat
     1861 tttatcataa caaagctaaa aacattaaag catgttgtaa aaaactcatt gatgaattta
     1921 atgagataat acctgataat attgatgaat tgataacttt acctggtgtt ggaagaaaaa
     1981 ctgcaaatgt aataattgct gaggtatttc ataaacctgc gattatagta gatactcatg
     2041 caaaaagatt atcaaacaga ataggtttga gtgataacac tgaccctgac aagattgaac
     2101 aggatttaaa ggaaatttta cctcctgagg aatcttcaaa tttctgtcac agattagttt
     2161 ttcatggaag aaaagtatgt aacgcaagaa aaccaaagtg tgatttatgc gtaattaata
     2221 aatattgtag atattttaac cagaaaaaga tataattact aaaaattaaa cataaaatat
     2281 aaatattaat aaaaatttaa tgttaattat ttaggaactt tatataatat gcatacgtct
     2341 atttaaagaa atgcaattgc aaaggtgcta taaaagtgaa tagtttagta aaagaaaaaa
     2401 atgatatgaa aaagaaaaac acaaagggtt ttgaaaagag agataaaagc tctacaatgc
     2461 cttttatgat acctagtatt gcaggttttt tacttttttt cctcattcct ttttttatag
     2521 tattatttta ttcttttata gataatccaa taaacaaaga atttgtaatg tttgataatt
     2581 atatttcatt acttaggaat catgcattta tgctttcttt aaagaatact cttattttct
     2641 cagttatagc agtccctcta gcagttataa tcccactgat cttagcattg tttcttgaaa
     2701 gtaatatacc aggaaaaagt atattcagaa cagcattgat aagtccttta gtagtaccag
     2761 tagcatccgt aatccttata tggcaagtag ttttccatta taacggcgtc ataaatgtat
     2821 ttttgaattc aataggtatt gatgctgttg actggttcaa atcagatttt agcagacttg
     2881 ttgttgtgtt gctttttttg tggaaaaaca caggttacaa tatgatttta tttatggctg
     2941 gtttagctaa tatcccgata gatgtaaaag aagcggcact aatcgatgga gtaggtccgg
     3001 taagaaggtt ttttagaatt aaacttaact atatgtcatc tagtattttc tttgtaacaa
     3061 tattgtcttt gattaattca tttaaagtat tcagagaagt ttacctttta actggtgatt
     3121 atccttatga gggattatat atgctgcaac attatataaa caacacattt agaacattgg
     3181 attaccaaaa actatctgct gctgcagtta taatttgcat aataataacg gctataattg
     3241 cattgttgtt ttacattgat aataaattgg ggagtagtgt tgatgagtaa aataggtatg
     3301 acaaaatcaa tgaaaataaa aagagttata tcaatatcta ttgcattggt tattctagct
     3361 tttactgcag ctatgtttat tatgcctatt gtacttacag taacaaattc atttatgacg
     3421 aaaagtgaaa taactgctaa ttatgggaaa atattttcag aaggtggatc atctgctgtt
     3481 tcatcttaca tagcagataa ggtaaatctg aaaattatcc ctgataaagt cagtttttca
     3541 caatatgtta ctgttttagt aaagagtcct cagtatcttt taaaattttg gaattcaata
     3601 atacttgttg taccaattgt catatttcag ataataattg cattaggtgc ttcatatagc
     3661 tttgcaaggt ataaaggcag atttagaaat acaatgtttt ttatctatat tgctcttatg
     3721 ctgatgcctt atcaggttac tttggtacca aactatcttg tggctgactg gatgaatatt
     3781 ttagaaacaa gatggtctat aatacttcca ggtatatttt caccgtttgc agtttttatt
     3841 ttgactaaaa caatgcagag aattccttct tcaattgttg aagcagctaa aattgatggt
     3901 gcgaatgaat ggaaaatttt taggaaagtt tttattccta tgtctaaaaa cgctatatac
     3961 tcagtggcaa tcttactgtt tatggattat tggaacatgg ttgagcaacc acttatcatg
     4021 ctgcaaaaaa gtgaggaaat gcacccgttg tctgtatttt tatcaaaaat aaatgctggt
     4081 gaaataggtc ttgcatttgc tgtagcagcc atatatatgg ttttacctct actattattc
     4141 ctttatagcg aagatagagt tttagagggt gtagcaagtt caagtgcaat aaaaggttaa
     4201 tggaggattt ttatgaaaaa agatgcatca aatagaagga atatagtaaa gaacatagct
     4261 atagttttcc tagcagttat gcttgttctt acattcttct caaatacaat tttgaattac
     4321 tcactgccgg aagtagctgt acaatatcca atgtacggaa ctatttcctc tagaataaga
     4381 ggtcagggta cagttacggc aaatcaaaca tatcaagtat attttgatga atcgagaata
     4441 atagaagaag tatatttcag aagaggagat atagttaaaa aaggtgatat actctatctt
     4501 ttaaaagatg ctccgagcga tgaactgaat aatgcaataa acactcttac tactatgaag
     4561 attaattatc aaagaaagct tatggaaagc acacctgatt atactgctat tctggaagag
     4621 atttcacaat tgaataaaca gctcattgaa gcaaaacaaa ttaagtctaa ccttaattca
     4681 aacagagcat tactgatttc tattcagact gaaattgatt tattaaataa gagtatttct
     4741 gaattatcta ctaaaaagac aaatcttgaa acatatctaa attcattgga gccagacact
     4801 tcattaagtg aagatgaatt tttagctgca ttaagaaatg cttctattga ttatgagaat
     4861 gccaagactg ctttggatgc agaaaaagag aatttaaaag aattgcagag agaattagtg
     4921 aaaatggcaa acccattatt agtagcacaa gctaatttag atgcaaaggt actggaaaaa
     4981 agtaattaca taaaagaaaa tccttctaca gatgtaccaa cttcatctga gataatgtct
     5041 aaggaaaatg atttggttac tgctgaaaat gcttctcctt ctgatcctgc tgaaataagc
     5101 agacttagaa atgaactaaa tagtatgata gctaaatatt ttgagtatat acctattcag
     5161 gaaagaataa atcaatacga tatcgaaata tcagtgttaa cagaagagtt gaataatatt
     5221 caaaggcaaa gaaatgaaat aaatacgaat atagagatat ctcaaaacag aattgatgta
     5281 ttacaattta catttgagga tatcgaagct gtatatcttc ctcttaaagc gcgttatgaa
     5341 tatgaagata tagagataga gattcaagaa gttagtaaaa cattattgga aagtcaggat
     5401 gaattacgaa tccttagtga agagaaaatt gaagttgaag gaaaaatcat gacagaatct
     5461 gaagctgatg aattgatagc tgtactggaa aagagtttat atgataaaca gcaatcatat
     5521 gagaaacaaa aagcagagga tgtgtttgat cagcaattga agcaaattga acttcaaaac
     5581 atgaaaagag aaattgaagt tcaggaagct ttagttgaaa gattgagtga taaagttgaa
     5641 aataacacaa taactgctcc tgttagcggt gcattattga atatgagata tgttgctgga
     5701 gagaaaactg aagcaggcgg agtagctgct gagatagaaa ttagtgaaaa aggttataca
     5761 atgagttttt ctgttactgt agaacaagca agaagaataa gtgttggtca acaagctgaa
     5821 attcagtatt actatggctc tacagctcct tatgctcaag taacaggtat tcaaactgat
     5881 tacaataatc ctcaatcaag cagaataata gtacttgaag taataggaga taatatatac
     5941 cctggtcaga ctctttatgt tgcacttgga gaaactggaa gagactttga aactgtagta
     6001 ccaaatagtg ctgttcatga agacaacaaa ggtaaatttg tatatattgt tgaagcaaaa
     6061 aacactccta taggtaacag gtatattgca aagagagttg atattcaggt tatagcttct
     6121 gacgataaga attcagctat atcaggtata agcgcatatg caaactatgt tattacaaca
     6181 gcttcaaaac cattaacaga cgggatgcag gtgaggttag tagaagaata gaatgaaatt
     6241 taaagcaaaa ctgtcatcaa aaatagttgt ttttataatt atcttatcag ttttgatagt
     6301 atcttttatg atattactta accagtcaaa taaaatagta aagaacaggt ttgatcaatt
     6361 tggtgcagaa agatggatga ataatgattc attgtttaac tatgctcaaa tttcatgttt
     6421 tttcacctct gaaactggtt ttagtgagaa taatatatat tcattaagcc gttcttacga
     6481 tgaaaaaatg atgtctgagt caatttcatc ttcagaaggt ggaagattat ggctgcatgc
     6541 atacagcgcg aaaggttctg taagtgttga tgcttcatca aatatcgcaa cagatgtaac
     6601 tgcggtagca ggagactttt tctttattca taatgttcca ttgataacag gttcgtattt
     6661 taaagatgat cctttaaata atgatcatat cttaattaat gaaacattag catggcagct
     6721 gtttggttct tttgatattg ttggaagaga tatttatatt tatgataagc cttattctat
     6781 aagcggagta acaaaaaacg atggagatgt aattgacgat tctctacctc atttatacat
     6841 gcagtatgat gtttataaag tactagataa tttagcatat atatcatgtt atgaagcaat
     6901 attgcctaat cctatttctg attttgcatt aaatgtagtt acatcattat tttcttttaa
     6961 tgaactagag tatgagataa tacaaaatac ttcacgattt aatttaatta atacttttaa
     7021 aaatttgata aatctgactg aaagaaatat taagacaaat cgagttatat atccacaatg
     7081 ggagaatcag gcaagaatta ctgagcataa actagcatat ttgttattgg ctcaaattat
     7141 acttattttt ctatttatat gtactttaac aattatgata ataatttaca gaagtaagat
     7201 tagtttattt tttgaaaata atagttcaaa aatagttact aaagctaaaa attccaaatt
     7261 agcagataag ataaggaaca aaaggagaga aagatatgaa aaaaagaaac ttagttaaaa
     7321 caatggtaat agtaggtctt gtaattgtaa tgctattaca gcttgtttca tgtaacaata
     7381 agaaaggtgc tgaaaaaact gttcttgatc acgtatttag aaggacagaa attactttac
     7441 ctgataatat tcaatctgtt aataatatat taaatgtagg tgataggatt aatatcattg
     7501 gttcaggata tgatcaggaa acatatgaaa attattatgt attctacagt ataaatcaag
     7561 atggtacaga ccctcaggag cacatgctta atcttaattt atccaaaatg gaaggttatg
     7621 aaggctcata tatgtccaac ataatcatgc ttcctaatga aaagtttcta gtatctattg
     7681 aagcatgggg atcagataag atatctggaa actacaaatc agataatttt cttgttatga
     7741 tggatttaga aggaaacatc gacaagaaag taaatgtaag agagttactt gaagaccact
     7801 tagaccagga ttatttttat atcaacaata ttgttgttga ttcacaaagc aatatatata
     7861 ttaattctga ccgaactgtt tttgttttag atgagaattt caatttttcg tttaaagttg
     7921 aacttaatga aaattcatgg cttaacagac tgtttgttta ttcagatggt aaaattgcag
     7981 ctgcaattaa ttctgaggga
//
LOCUS       DAOSXM010000093         7934 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2111
            NODE_5863_length_7934_cov_10.667470, whole genome shotgun sequence.
ACCESSION   DAOSXM010000093 DAOSXM010000000
VERSION     DAOSXM010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864178
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 7934)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7934)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:24:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,261
            CDSs (total)                      :: 2,229
            Genes (coding)                    :: 2,218
            CDSs (with protein)               :: 2,218
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 3 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7934
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5863_length_7934_cov_10.667470"
                     /isolate="AMR_MDS_2111"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1427)
                     /locus_tag="PLQ68_09535"
     CDS             complement(<1..1427)
                     /locus_tag="PLQ68_09535"
                     /inference="COORDINATES: protein motif:HMM:NF014900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase family protein"
                     /protein_id="HXK72935.1"
                     /translation="MGNSFTLSGRLEALLSSKKNQTEKKYAKFGRTQDMDDRGNLVAP
                     DSFIFTPVPNHESSGFFGPYACGMNYKKLLHEHPVTIDENAGLLGSWMVMLLEYKKPL
                     WNPDINYEHLHKEQKLYDIVHGIGGVHHFLPDYSLGLKLGFKGIKQKIKKYEKDGDSS
                     KIEFYKGLNECVEGIFEYIKRHIQKARQVAADIKDIDKADNLLMLAEMNEELLKRPAK
                     TFHEAVQWMAWYLVCGNMYNGGASAVGDIDRILKPYYDNDIEKGILIKEQAIFYICNF
                     LIIDNAYCQIGGCDEFGKDNTNELSHVVLEAVHKMKIPASVCVRVHENIDEEFFRKAV
                     KYLLEDRTGSPNFISDKAMVEGFIKNGYSKELAVSRVKCGCHWCAIPGKEYTLNDIVK
                     INFVAVFDAAFKQMMEEQKEKASVKLLWQLFAKHLERAVTVTAQGMDFQIEHMHEVFP
                     ELLMDLLCHGPIEKGLDASNGGLEY"
     gene            complement(1504..2628)
                     /locus_tag="PLQ68_09540"
     CDS             complement(1504..2628)
                     /locus_tag="PLQ68_09540"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HXK72936.1"
                     /translation="MSLTSKERIMRIFQNKQVDRPAIKLWGAWKYQKGDFLLNPMYEP
                     VAKKASDITDLFIEARSPFNMYCGQLPDITREYVEIDNDSWVQRHTVYHTPMGDLRHV
                     EMISKFDEPGYTIEYPVKEPEDLKKILSMKYEPFPYSDNIEYKQQNLNDRGIVMYEID
                     HIGYALQRLTGSENLAYFSYDCRDLVLEVLDTFAKRQYNQVKRAIDNKVNVPFCWVGP
                     ELLTPPLMSENDFEQFCFNYDKPICDMVHNAGSYVWVHCHGKVRKLIERYILMGVDIL
                     NPLEPPKNGDVDLNETAKTFKGRIGLEGNIEIQDILLESEEKLKESIHSCVQSGKESG
                     RFILCPSAGFMEYPKPTQHYINNLMTYLDYGYECVNKAVY"
     gene            complement(2710..3024)
                     /locus_tag="PLQ68_09545"
     CDS             complement(2710..3024)
                     /locus_tag="PLQ68_09545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010244532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HXK72937.1"
                     /translation="MIEQIFKLSTDNTKAVEKVIFDDNLHYIHMVFNKEEGLPEHVSN
                     SNVYMTVVRGTLTIGLGDEKPNEYVKGTLLKIPKGVKMNVGNKNDETLELIVVKAPAP
                     EK"
     gene            complement(3111..3746)
                     /locus_tag="PLQ68_09550"
     CDS             complement(3111..3746)
                     /locus_tag="PLQ68_09550"
                     /inference="COORDINATES: protein motif:HMM:NF024634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HXK72938.1"
                     /translation="MNKTWITVIRIAFFGIFLFLMLIGKPFIWLALYGLSLILALFFG
                     RIYCGFMCPINTVMGPAEFLSKKMSIQTDKTPKWLSSGVFAWIFLALSIALMLIFKKA
                     LKIDLPIIPIWIAVGFLVTLRYKPHVFHNLICPFGTLQGIFGRKPLFSKKVDKETCIG
                     CRLCEKACPSDAIKVSSETKKATITPNICHQCTNCKDVCPKGSILYNNAKQ"
     gene            3825..4475
                     /locus_tag="PLQ68_09555"
     CDS             3825..4475
                     /locus_tag="PLQ68_09555"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HXK72939.1"
                     /translation="MINDKNIITGNISNTKYEQLLENNIIRIKKYSKGNFIHMENDLC
                     TLLEIIIEGNVSIDRIDEEGNVMCIAKFTKNDILGGNLLFSRTPAYPMSIIANTDVTM
                     LQIKKDDLFNLMCHYPDFLKAYLENISDNAFILGDRIRGYVKTTLRQILTNYLKKQSN
                     IQKSNTIKLNMTKKELAFLLGVQRTSLSRELAKMRNDKLITFNKNEIKIINFEKDY"
     gene            4545..4841
                     /locus_tag="PLQ68_09560"
     CDS             4545..4841
                     /locus_tag="PLQ68_09560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003870834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5320 domain-containing protein"
                     /protein_id="HXK72940.1"
                     /translation="MPRFDGTGPMGMGPMTGRGLGYCTGVRRPLGAGYGGGFYGRGRG
                     GGFGYKRGFFGYSRPVYYDNPQYAEKPSKELLLDQKKYLEEELASINKQIDELK"
     gene            4877..5245
                     /locus_tag="PLQ68_09565"
     CDS             4877..5245
                     /locus_tag="PLQ68_09565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013842012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF134 domain-containing protein"
                     /protein_id="HXK72941.1"
                     /translation="MARPRKWRTVCDLPQINRFGPMSSKDAVLETVIMAVDEYETLRL
                     IDLEGFTQEQCADQMNIARTTIQGIYSEARRKLADSLVNGKNLVIEGGDYMLCNGMGR
                     GCQMQGCRRHRHGNGHNNEF"
     gene            5255..5617
                     /locus_tag="PLQ68_09570"
     CDS             5255..5617
                     /locus_tag="PLQ68_09570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015943770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HXK72942.1"
                     /translation="MKIAIPADEKNLDSQVCISFGRAPYYLYYDLESDKAEFVENVAA
                     SAPGGAGIQAAQSIIDNKADVLLTPRCGENAARVIHSSGIRMFKTKSGSIRENIEEFK
                     NNNLESLSEIHPGFHKHG"
     gene            5626..6468
                     /locus_tag="PLQ68_09575"
     CDS             5626..6468
                     /locus_tag="PLQ68_09575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014825363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXK72943.1"
                     /translation="MKIAVLSGKGGTGKTFVTVNLAYANNSSVYIDCDVEEPNGHLFF
                     KPKINNKKDVCVKIPVCNDDLCDGCKKCVSFCEFHALAYINNKLMLFDKICHSCGGCE
                     IICPQKAISETDKVIGHYEYGYSGKTFIKTGYLKTGETTGIPIIKQLLKDDINSKDKD
                     IFIDCPPGSSCSVIESIKDSDYCVLVAEPSVFGAHNLQMVHELVKIYNKPYGVVLNKC
                     TKDYNPSYEYCKSNNIDILESIDFDKELGLLNSNGFIAAKESTFYFDLFVSLLEKISK
                     GAKI"
     gene            6465..7343
                     /locus_tag="PLQ68_09580"
     CDS             6465..7343
                     /locus_tag="PLQ68_09580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006440422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXK72944.1"
                     /translation="MKQLLILSGKGGTGKTTVASAFISLSKAKAYADCDVDAPNLHLV
                     NHNDETYSTKDFYGLKKANIDPDVCIGCGLCYDHCRFYAIIEGDKYSVDINACEGCAV
                     CGLVCPVNAITMEPNIVGDLMLYKNEKRVFSTAKLHPGNGNSGLLVSEVKKQMKDNSL
                     KDTNFAVIDGSPGIGCPVIASLSAVDMVLIVAEPSLSGFSDMERIISTARTFETDIIL
                     CVNKYDINTKITQQIEEMCAKENIIFVGKIPYDTSVITAINNNKPVTDFEGKASDEIK
                     LIYKKVIDLLLDEAQL"
     gene            7340..>7934
                     /locus_tag="PLQ68_09585"
     CDS             7340..>7934
                     /locus_tag="PLQ68_09585"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXK72945.1"
                     /translation="MIIKTLVEDTTDKENLKSEHGLSLYIETNGKKILFDSGASGLFM
                     ENAEIMDIDIASIDYAIISHGHNDHGGGLGKFLEANSKAEVFIHEHAFDRHYSLRNGR
                     MVYIGLDENLSKNDRLVYVKGRFFIWKGIELFSDVKTLKPMPVTNSNLYAEDKGDIIA
                     DNFSHELNLAITEDNNHFLLTGCAHNGIMNIMEHYVEL"
BASE COUNT         2709 a         1366 c         1314 g         2545 t
ORIGIN      
        1 atatattcca accccccatt agaagcatcc aaacccttct caattggacc atgacataat
       61 aaatccatta aaagttcagg aaatacttca tgcatatgct caatttgaaa atccattccc
      121 tgtgcagtaa ctgttactgc tctttctaga tgcttagcaa ataattgcca caataatttt
      181 actgatgctt tttccttttg ttcttccatc atctgtttga aggcagcatc aaaaacagcg
      241 acaaaattta tctttactat atcattaagt gtatattctt ttcctggtat cgcacaccaa
      301 tgacatccgc atttgacccg acttacagca agttcttttg aatatccatt ttttatgaat
      361 ccttcgacca ttgccttatc tgatataaaa ttaggagaac cagttctatc ttccaataga
      421 tatttaacag ccttgcggaa aaactcttca tctatgtttt catgaactct tacacataca
      481 ctggcaggta tcttcatttt atgaactgct tctaaaacaa catgagaaag ttcatttgtg
      541 ttatctttcc caaactcatc acaaccgcca atctggcaat aagcattatc gattataagg
      601 aagttgcaaa tatagaatat agcctgttct ttaatcaaaa tccccttctc tatatcattg
      661 tcataatacg gtttaagaat cctgtcaata tctccaactg cactagcacc gccattgtac
      721 atattaccgc ataccagata ccatgccatc cattgaactg cttcatgaaa agttttagct
      781 ggtcttttta atagttcctc gttcatttct gcaagcatta aaagattgtc agctttatcg
      841 atatctttta tatctgctgc aacttgacgg gctttttgga tatgcctttt aatgtattca
      901 aatatacctt ctacacattc attcaagcct ttataaaatt ctatttttga agaatcacca
      961 tctttttcgt attttttaat tttttgctta atacctttaa atccaagttt caatcctaat
     1021 gaataatctg gtaaaaaatg atgtactcca cctataccat gaacaatatc atataacttc
     1081 tgctctttat gtaaatgttc ataattaata tcaggattcc ataaaggctt tttatattct
     1141 agaagcataa ccatccagct tccaagaaga ccagcatttt cgtctattgt cacaggatgt
     1201 tcatgtaaaa gttttttata attcataccg cacgcatatg gtccaaaaaa accactgctt
     1261 tcatgatttg gtacaggagt aaaaataaaa ctgtcaggcg caacaagatt tccgcgatca
     1321 tccatgtcct gagttcttcc gaattttgca tacttttttt cagtctgatt ttttttagat
     1381 gataataaag cttcaagtct gccagataaa gtaaaactgt tccccatttt tccatccttc
     1441 aaatatattt tcacacctag aagtaatcat atcattaagc ataatattgt caataataac
     1501 caactagtat actgctttat taacgcattc atacccataa tccaaatatg tcataagatt
     1561 gtttatatag tgctgggtgg gtttaggata ttccataaaa cctgctgacg gacacaaaat
     1621 aaatcttcca ctctctttac cgctttgaac gcatgaatgt attgattctt ttaatttctc
     1681 ttctgattca agtagtatat cctgtatttc aatattacct tccagtccta tccgtccttt
     1741 aaatgttttt gctgtttcat ttaaatctac atcaccattt ttaggaggct ccaaaggatt
     1801 aagtatatcc actcccataa gtatatatct ttcaatcagt ttcctgacct ttccatggca
     1861 atgcacccat acataacttc ctgcattatg aaccatatca caaatcggtt tgtcataatt
     1921 gaaacaaaac tgttcaaaat cattctctga cattaaaggc ggtgtcagaa gttcaggtcc
     1981 aacccagcaa aagggtacat tcactttgtt atcaattgct cttttaacct gattgtactg
     2041 tctttttgca aatgtatcca gcacctccag tacaagatcc ctgcagtcat aactgaaata
     2101 tgcaagattc tctgatccag tcaaacgttg caaagcataa cctatgtgat ctatctcata
     2161 cattactata cccctgtcat ttaagttctg ctgtttatat tcaatattat cagaatatgg
     2221 aaaaggttca tatttcatgg aaagaatctt ttttaaatcc tcaggctctt ttacaggata
     2281 ttcaattgta tatcctggct cgtcaaattt cgaaatcatt tcaacatgtc gcaaatcacc
     2341 cataggcgta tgatatacag tatgccgttg cacccatgaa tcgttgtcaa tttctacata
     2401 ttcccttgta atatcaggta actgtccgca atacatattg aaaggagatc ttgcctcaat
     2461 aaataaatca gtaatatcag aagccttttt tgcaacaggt tcatacatcg gatttagaag
     2521 aaaatcacct ttttggtatt tccatgcacc ccatagttta atagcaggtc tgtcgacctg
     2581 cttattttga aatatcctca ttattctttc ttttgatgtt agtgacatat ataccgcctc
     2641 tgtttcagtt aatatgatta tgccttattt tgtacaatca tacaactgat attgaggaat
     2701 ttttgatttt tacttttccg gtgcaggagc tttaactaca ataagttcaa gtgtttcatc
     2761 atttttattt ccgacattca tttttactcc ttttggtatc tttaaaagag ttccttttac
     2821 gtattcattt ggtttttcgt caccaaggcc gattgttaat gttcctctta caactgtcat
     2881 ataaacattt gaatttgaga catgttctgg aaggccttct tctttattaa ataccatgtg
     2941 tatataatgc agattatcat caaatataac tttttcaact gcttttgtat tatctgtaga
     3001 caatttaaat atctgttcta tcatataact acctccaata ttatttaatg atattatatt
     3061 tcacaatgac agaatttgca gtaacaaagt taccataaat tcattcaaaa ctactgcttt
     3121 gcattgttat ataggattga gcccttcggg cagacatcct tacagttagt acactgatga
     3181 caaatattag gcgtaatagt ggcctttttt gtttctgatg atacttttat agcgtctgaa
     3241 ggacatgctt tttcacacaa cctgcaccca atacaggttt ctttatcaac ttttttcgaa
     3301 aaaagcggtt ttctaccgaa aataccctgt aatgtgccaa atgggcatat aaggttatga
     3361 aatacatgag gcttatatct taatgttaca agaaaaccta ctgctatcca aattggtatt
     3421 attggcaaat cgattttcaa agcttttttg aaaattagca ttaaagcaat acttaatgct
     3481 aaaaatatcc atgcaaaaac acctgaagac aaccatttgg gcgttttatc tgtttgaata
     3541 ctcatctttt tagataaaaa ctcagctggc cccattacag tattaatagg acacataaaa
     3601 ccacaatata ttctgccaaa aaacaatgcc aatataaggc ttaatccata taaagccagc
     3661 catataaaag gtttgccaat cagcatcaag aataaaaata ttccaaaaaa tgctatcctt
     3721 attaccgtta tccaagtctt gttcataaat acctccacat tttgtataag tatactttat
     3781 aaagatattt aaatgtgtta cattggtaac acgtggagga taacatgata aatgataaaa
     3841 acataataac gggaaatata tcaaatacaa aatatgaaca actattagaa aacaatataa
     3901 tacgcataaa aaaatattct aaaggcaact ttattcatat ggaaaatgac ttatgcactc
     3961 ttcttgaaat tataattgaa ggtaatgttt caattgacag aatagatgaa gaaggaaatg
     4021 tgatgtgtat tgcgaaattt acaaaaaatg atatcctggg aggtaatctg cttttttcca
     4081 gaacacctgc ttaccctatg agtattattg caaatactga tgttactatg ctgcaaataa
     4141 aaaaggatga tcttttcaat ctgatgtgtc attatcctga ttttttgaaa gcatatcttg
     4201 aaaacatatc tgataacgca tttattctcg gagacaggat tagagggtat gtgaaaacaa
     4261 cattaaggca gattctcact aattatttaa aaaaacagtc aaatatacag aaaagcaata
     4321 caataaaatt aaacatgaca aaaaaagaac tagcttttct tttaggagtt caaagaacct
     4381 ctctttccag agagcttgca aagatgcgta atgataaact gataaccttc aataaaaatg
     4441 agataaagat aataaatttt gaaaaagatt attgacatat gtcattaatg atgtatacta
     4501 ataatatctg acatatgtcg gaaatgaaac aggaggttat agaaatgcca agatttgacg
     4561 gaacaggacc aatgggtatg ggtccaatga ccggtagagg attaggatat tgcacaggag
     4621 taagacgtcc tttaggtgca ggttatggtg gaggttttta cggcagaggc agaggaggcg
     4681 gtttcggata caaaagagga tttttcggat attcaagacc tgtatattat gacaatcctc
     4741 aatatgcaga gaagccttca aaagaacttc tattggatca gaagaagtat ctcgaagaag
     4801 aacttgcatc tatcaataaa cagatagatg agttaaaata aaccttaagg acaattaaga
     4861 acaagaaagg ttacctatgg caagaccacg aaaatggcgt acagtatgtg acctgcccca
     4921 aataaaccgt ttcgggccta tgtcttctaa agatgcagta ctcgaaacgg tgataatggc
     4981 ggtagatgaa tatgaaacat tacggcttat tgatcttgaa ggattcactc aggagcaatg
     5041 tgctgatcag atgaatattg cccgaacaac aatacaaggt atttatagtg aagctagaag
     5101 aaaacttgca gattctttag taaatggaaa aaatctggta attgaaggtg gcgactatat
     5161 gttatgtaat ggaatgggcc gaggatgtca gatgcaaggt tgcagacgac acagacatgg
     5221 taacgggcac aacaatgaat tttaggagga atatatgaaa atagcaatac cagcagatga
     5281 aaagaattta gattcacaag tatgtatatc ttttggaaga gctccatatt atttatatta
     5341 tgatttagag agtgataaag ctgaattcgt agagaatgta gcagctagtg ctcctggcgg
     5401 agcaggaatc caagctgccc aatcaataat tgacaataag gctgatgttc ttttaactcc
     5461 aagatgcggt gagaatgcag ccagagtaat tcattcatct ggtatcagaa tgtttaaaac
     5521 aaaatccgga tcaataagag agaacatcga ggagtttaaa aacaacaacc ttgaatctct
     5581 atctgaaata catcctggtt ttcataaaca cgggtaatta gtattatgaa aattgctgta
     5641 ttgagtggca aaggaggtac tggcaaaacc tttgtcaccg taaaccttgc ttatgcgaac
     5701 aatagttctg tttatattga ttgcgatgtt gaagaaccaa atggacatct gttttttaaa
     5761 cccaaaatca ataacaaaaa agatgtctgt gttaaaatac ctgtttgtaa tgatgactta
     5821 tgtgatggat gcaagaaatg cgtttcattc tgtgagttcc atgcattagc atatataaat
     5881 aataaactta tgctgtttga taaaatctgc cactcatgtg gtggatgtga aattatctgc
     5941 ccgcaaaaag cgatatcaga aactgataaa gtaataggac attatgaata tggctattca
     6001 ggtaaaactt ttataaaaac tggctatctt aagactggag aaaccacagg aattcctatc
     6061 attaagcaat tattaaaaga tgatattaac agtaaagata aagatatatt tattgactgc
     6121 cctccaggaa gctcttgttc agttatagaa agcataaaag attctgatta ctgcgttctg
     6181 gtagctgaac cttctgtttt tggagctcac aatcttcaaa tggtacatga actggttaag
     6241 atttataaca aaccatatgg agtagtattg aataagtgta caaaagacta taatccttca
     6301 tacgaatact gcaaatctaa taatatagat attttagaat caattgattt tgataaagag
     6361 ttaggattat tgaactctaa tggatttatt gcagccaaag agagtacttt ttatttcgac
     6421 ttattcgttt ctttacttga aaaaataagc aaaggagcaa aaatatgaaa cagcttttga
     6481 tactcagcgg taaaggcgga acaggaaaaa caacagttgc atctgcattt atatccttgt
     6541 caaaggcaaa agcatacgct gactgtgatg tagatgctcc gaatcttcac cttgttaacc
     6601 ataatgatga aacttattca acaaaagact tttatggttt aaagaaagca aatatagatc
     6661 ctgatgtatg tataggatgc ggattgtgtt atgaccattg tagattttat gctattattg
     6721 aaggcgataa atacagtgtc gatataaacg cttgtgaagg atgtgcggta tgtggtcttg
     6781 tctgtcctgt aaatgcaatt acaatggagc ctaacattgt tggagatttg atgttatata
     6841 aaaatgaaaa aagagtgttt tctactgcaa agcttcatcc aggcaacggc aattccggct
     6901 tactggtgag tgaagtaaaa aaacagatga aagataactc ccttaaagat acaaattttg
     6961 cagttattga cggatctcca ggaataggat gtccggtcat tgcttcatta agtgcggttg
     7021 atatggtctt aatagttgca gaaccttccc tatcaggatt ttcagatatg gaaagaataa
     7081 ttagtactgc aaggactttt gaaactgata ttattttgtg cgtaaacaaa tatgacataa
     7141 atacgaaaat cacacaacag atagaagaaa tgtgtgcaaa ggaaaatata atttttgttg
     7201 gaaaaatacc atacgataca tcagtaatta ctgcaatcaa caacaataag cctgttactg
     7261 attttgaagg aaaagcttcc gatgagatta aactaattta taaaaaagtt atagacctac
     7321 ttcttgatga ggcgcaatta tgattattaa aactctagtt gaagatacta cagacaagga
     7381 aaacttaaaa tcagaacatg gcttaagtct atatattgaa acaaacggca aaaaaatact
     7441 ttttgattca ggcgcaagcg gactttttat ggaaaatgca gaaataatgg atatagatat
     7501 tgcaagcatt gattatgcaa tcatatcaca tggtcataat gatcatggtg gcggtcttgg
     7561 aaaattccta gaagctaata gcaaggctga ggtttttata catgaacatg cttttgacag
     7621 acattattca ctcagaaatg gaagaatggt ttacatcgga cttgatgaga atttaagcaa
     7681 gaatgatagg cttgtttatg tcaaaggccg attctttatt tggaaaggga ttgagttatt
     7741 ttcagatgta aaaacactta aaccaatgcc tgtcacaaac agcaatttat atgcagaaga
     7801 caaaggtgac ataattgcgg ataatttctc tcatgagcta aatcttgcaa taacagagga
     7861 taacaatcat tttcttctca caggatgtgc tcataacgga ataatgaata ttatggagca
     7921 ttatgtggaa ttaa
//
LOCUS       DAOSXM010000094         7862 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2111
            NODE_5934_length_7862_cov_11.057897, whole genome shotgun sequence.
ACCESSION   DAOSXM010000094 DAOSXM010000000
VERSION     DAOSXM010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864178
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 7862)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7862)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:24:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,261
            CDSs (total)                      :: 2,229
            Genes (coding)                    :: 2,218
            CDSs (with protein)               :: 2,218
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 3 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7862
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5934_length_7862_cov_11.057897"
                     /isolate="AMR_MDS_2111"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..632)
                     /locus_tag="PLQ68_09590"
     CDS             complement(<1..632)
                     /locus_tag="PLQ68_09590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72946.1"
                     /translation="MKRRTKILALILCVCMLSISLFACSKPQNPEKGYYLTGSFNGYA
                     VADEAYKMIKVSDESMLKEAMPDAELYYFIVNAENLTPDSVYGQHYYKVTAGNWDNAW
                     GTEAYDLQPAAVYYDADGNPTGLGSIYIPGDYKGELIVVFDASAKKIYDNTMIKEFTP
                     RVYGDFQTSFGLKNDWDYSDEGSYIMQKNSQGQYEVTIEVPAYIPEGEGAT"
     gene            complement(649..2346)
                     /locus_tag="PLQ68_09595"
     CDS             complement(649..2346)
                     /locus_tag="PLQ68_09595"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HXK72947.1"
                     /translation="MNRHAVYHISRGAYAYAIGEYTLCVRLRTAKNDCTTVKVHYLNV
                     YNHSNNRKKVSMEKILQDEYYDIYEAQLKLEERHFKYFFELKNDKESIYYTSDGFISN
                     DVKNENSFFFPVINDDEIYTFPKWAEGVTIYQIFVDRFNKTYEKPFINAKWNDIPKRH
                     LFYGGDFNGIKEKLDYIHSLGVDVIYLSPIFSSVTNHKYDIEDFYSIDEMFGGEDQLQ
                     DLIKEIHNKKMRIVLDCVFNHMSCYNKIFQDVIEKGKKSLYKDWFYIKEYPISIDRCN
                     YDTFAGCVPSMPRLNTSNPQVVDYLVGSSVYWTKKLGIDGWRLDVADEVSVSLWRKFR
                     EEILKANCEALIIGEIWNDASKWMNGDQLHTVTNYKYRKWMMEFIENKIDSSVFWQKI
                     SANNALYKTLSYNYLVNLLGSHDTKRLASVVGKNKAILALLTTLVFQGIGLIYYGDEI
                     GLTGKDDPDNRRTMPWDKVSDENIKPFELLAKLKNKYPCIKYGKLIPIIVNENILAFE
                     RSYKNKNVEVYVNFTNKDILIESRKSIFHYSFGEMIDEKTMSINRIRALSFAICIKK"
     gene            complement(2343..3176)
                     /locus_tag="PLQ68_09600"
     CDS             complement(2343..3176)
                     /locus_tag="PLQ68_09600"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HXK72948.1"
                     /translation="MPKKKRKYSKIGVHFILIALALIVIIPFAYILLISFGKDMLDIS
                     GYGDSGFSFEYYEQLFSETKYLFWLGNSLLISICTMVISLIMVSIASYVFSRFKFAGR
                     EALFQTILLIQIFPLTLMLVSLFKIFASLELLNRLIGLVIIDSVIATAGLILVSKGYF
                     DTVPYEIDEAAMIDGANKFQILVHITLPLVKPILGIVAIQSFVLTYNEYAIASMLITS
                     GAEKMTVAVGLQSLIYGQFQQNWGMYCAGAVLASLPIFILFYSAQKYFIGGLTEGGVK
                     E"
     gene            complement(3181..5508)
                     /locus_tag="PLQ68_09605"
     CDS             complement(3181..5508)
                     /locus_tag="PLQ68_09605"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HXK72949.1"
                     /translation="MKNRVICLLTIILTLISFFFIYPVNAQTNKVIRVWGSEITREND
                     VLRNIAESYKEKTGTDVLIVDRRSLFDAPKDLINNAQTQERPDIVMIQHADIGDLAVN
                     GFLMELDFSESIKSAFDESAFTSFEVNDKQFGIGYSVETYGLVYNKDVISYDQISDLT
                     WDEFFEFSKNFGTIEHKGSQMKAFILNPKNMYFMYPIIRNYGGYYYGFDEEGNYNPYD
                     IGLNNEGSKIYVNKIKELMQFDLVARSRNCTDNDVSAAFANNQSAMIINGFWQNSFYT
                     QKDVNFGFAPLPKNNDNTTSAPYATINGFVINAHTNFPDEAIAFLEFLCKDENQQKMI
                     EAGNNFERKIGSRFPVNLSVQQSKYITSEPMYNVMAQICKNVEPVPNIPEGDIWYNYT
                     DKAYQSIFYGDINGNPLDSDNMLDELASAILNDVRKMNVQVEKVDISLWGYIFLGSFV
                     VIGLGILVITIIRKRKKFSASANRIMLRATTISYVVLLPIIGLIGIFYVFPIVHNIYL
                     SMTNYSSLNLVDYVFVGGYNYKTIFIEGLNGFVAMFLWTIIFAACVMTSTFILGTLLA
                     TLLEKRNATIAKIYKMIFILPWVIPSVIMLLTWQGILATDGGALNNLLNILGFGKVMW
                     LDNPYLARLSTIFVVSWMGTPYYMFLALGFLKSIPKDYYEAAMIEGAGSVRVFFTITV
                     PIIFRTLLPTLIMGFIMQFNNFGVYLLTSGGPFGSNLGAPGATDLLITYVYNLAYNTK
                     NYSLAAAYSVIIFVFVASFSLITMRISNKKFNEGK"
     gene            complement(5522..6589)
                     /locus_tag="PLQ68_09610"
     CDS             complement(5522..6589)
                     /locus_tag="PLQ68_09610"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HXK72950.1"
                     /translation="MKHITQLKPSIAKIASEMSKTYSDALNHINGNVTFRALPDSDIK
                     EYLNLAAYKYNETDLDIYVDNLLKGHFSMMEARKSVEDNYIPQLNINLGIGDYAAFVA
                     GDVYFKKDTSWSLPIITDLKMYKDLPPIGTSKWYRKYMYILERVLSKTKDTDIPFARG
                     FFSPLDLAEALHGSEIYTDFYDNPDKLHELLDYCADVTIKFAEDIYGLIRKYRKNPTY
                     GTMFIDGIINMSEDIASMISPDLYDEFCAPHTQKIISHFGSGHMHSHSCAIYLVKHIC
                     DLKNVTNLWLATDPNHPRPFDMLDQLIEDANGTCLAIDCNSFEEIESNIDILKKGNFS
                     VCLPVRTIEEAISYTEKFRSL"
     gene            complement(6669..7559)
                     /locus_tag="PLQ68_09615"
     CDS             complement(6669..7559)
                     /locus_tag="PLQ68_09615"
                     /inference="COORDINATES: protein motif:HMM:NF012576.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HXK72951.1"
                     /translation="MVKKYTMQDIAKAANVSKATVSYVLNDKKNARISDETRRRILQI
                     ANLYQYVPNLSAKYLCDNRKKLIGIIIGDSSCKSFISECKIIYMLYEIFHADDYKLVL
                     LDNKRQDNYMDLAYDLILAINISEEQIRNIGINTYSPMILFDSMFSDNLFFKLINNYQ
                     KAVEKAITLFDNEKECVLIYSLRNNKGINEKIEDIPLKKIAFTGENRKDIDVFVKNNI
                     EKNFIAIGDIPGFIALSAIRYNQLILITFSENVAFPVEVKQVVFDSQVLKDYIKIIFK
                     KLDTRQAVEKEQHIFLIDPS"
BASE COUNT         2605 a         1471 c         1031 g         2755 t
ORIGIN      
        1 gtcgcacctt caccttctgg tatataagca ggtacttcga tagtaacttc atattgacct
       61 tgtgaattct tttgcattat gtatgatcct tcatcagaat agtcccagtc atttttcaat
      121 ccaaatgaag tctggaaatc accgtataca cgaggagtaa attctttaat cattgtatta
      181 tcatatattt tctttgcact agcatcaaaa acaacaatga gttccccttt ataatcacca
      241 ggtatataaa ttgaacctag tcccgtagga ttaccgtcag catcataata tactgcagct
      301 ggctgaagat catatgcttc agtcccccat gcattatccc aattacctgc agttacttta
      361 tagtagtgct ggccatacac agaatctggt gtaagatttt cagcattaac tataaagtag
      421 tacaattccg catctggcat tgcctctttt agcatacttt catcacttac ctttatcatc
      481 ttatatgctt catcagctac agcataacca ttaaagctac ctgttaaata gtaacctttt
      541 tccggattct gaggtttcga gcatgcaaat aaagatatag ataacatgca tacacataag
      601 ataagtgcta aaatttttgt tctcctcttc ataataaatt ccacctttct acttttttat
      661 gcaaatcgca aaggataatg cccttatacg attaatgctc attgtttttt catcaatcat
      721 ctctccgaat gaataatgaa atattgattt tctgctttct attaatatgt ctttatttgt
      781 aaaattgaca tacacttcaa cattcttatt tttataggaa cgttcaaatg cgagaatatt
      841 ttcattcact attatcggta tcagtttgcc atacttaatg cagggatact tattctttaa
      901 ttttgctaat aactcaaaag gttttatgtt ttcatcagat accttgtccc aaggcattgt
      961 tcttctgtta tcagggtcat cttttcctgt aagtccaatt tcatcaccgt aatatatcaa
     1021 tcctattcct tgaaaaacca atgttgttaa caatgctaaa atagctttat tcttaccaac
     1081 aactgaagct aatctttttg tatcatgact tcccaggaga ttaactagat agttatatga
     1141 aagagtctta tacagagcat tatttgcaga aattttctgc caaaaaactg atgaatcgat
     1201 tttattttct ataaactcca tcatccactt tctatatttg taatttgtaa ctgtatgcaa
     1261 ttgatcacca ttcatccact tggaagcatc gttccatatc tctcctatta tcaaagcttc
     1321 acaatttgct ttcaaaatct cttctctaaa ttttctccat aggctgacag acacctcatc
     1381 tgcaacatct agtctccaac catcaatacc aagtttctta gtccaataaa ccgatgatcc
     1441 gacaagatag tctacaactt gcggatttga agtattcaat ctcggcattg atggaacaca
     1501 tcctgcaaaa gtatcgtaat tacatctatc tatgcttatc gggtactctt ttatatagaa
     1561 ccaatccttg tataatgatt tcttcccttt ttcgattaca tcctgaaaaa tcttgttata
     1621 acagctcata tgattaaata cacaatcaag aacaatcctc atttttttgt tatgtatctc
     1681 tttaattaaa tcttgtaact ggtcttcccc accaaacatt tcatcaattg aatagaagtc
     1741 ctcaatatca tatttatgat ttgtaacaga agagaatatc ggactgagat atatcacatc
     1801 cactcctaat gaatggatgt agtccagttt ttcttttatt ccattaaaat cacctccata
     1861 aaaaagatgt cttttaggga tatcattcca ttttgcatta ataaagggtt tttcatatgt
     1921 cttgttgaat ctatcaacaa atatctgata tatagtaacg ccttctgccc acttaggaaa
     1981 agtataaatt tcgtcatcat tgattacagg aaaaaagaaa ctgttctcat tcttcacatc
     2041 attacttatg aaaccatcag aagtgtaata aattgattct ttatcatttt ttagttcaaa
     2101 gaagtattta aaatgcctct cttctaattt taactgtgct tcgtatatgt cataatattc
     2161 atcctgtaat attttttcca tacttacttt ttttctatta ttagaatgat tgtatacatt
     2221 aagatagtga acctttactg ttgtgcaatc atttttagct gttctcaagc gcacacataa
     2281 tgtgtactca cctattgcgt aggcatatgc acctctgctt atatgataaa ctgcgtgtct
     2341 attcattctt ttactccccc ttctgttaat ccaccaataa agtatttttg ggcagaatag
     2401 aataatataa atattggaag acttgctaat acagcacctg cacagtacat accccagttt
     2461 tgctgaaatt gtccatatat aaggctttga agaccaacag caactgtcat tttctcagca
     2521 cctgatgtta taagcatgct tgcaatagca tactcgttgt aagttaatac aaaactttgt
     2581 attgcaacaa tacctaaaat aggttttacc agtggcaaag taatatgcac aagtatttga
     2641 aatttattag ccccatcaat cattgctgct tcatctatct catatggcac tgtgtcgaag
     2701 taaccttttg atacaagaat caaccctgca gtcgctataa ctgaatcaat aatcacaagc
     2761 cctattagtc tattaagcaa ttccagtgaa gcgaatattt taaacaatga gacgagcata
     2821 agtgttagtg ggaaaatctg aattaacaga attgtttgaa ataaagcctc tcttccagca
     2881 aatttaaatc tagaaaacac atatgaagca atcgatacca taataagaga aattaccata
     2941 gtacatatag atattagtaa tgaattacca agccaaaaaa gatacttagt ttcagaaaag
     3001 agctgttcat aatattcaaa tgaaaatcct gagtctccat atcctgatat atcaagcata
     3061 tcctttccaa aagatataag cagtatatat gcaaaaggaa ttattactat tagtgctaat
     3121 gctattaata tgaaatgtac gcctatcttt gaatattttc tctttttctt tggcatttta
     3181 tcacttccct tcattaaatt tcttgttgct tatcctcatg gtaataagtg aaaatgaagc
     3241 aacaaaaaca aaaataatca cagaatatgc tgctgccaga ctatagtttt tagtattata
     3301 agcaagattg tatacatatg ttattagtag atctgttgca cctggtgcac ctaaattact
     3361 gccaaaaggt ccacctgatg taagtagata aaccccaaaa ttattaaatt gcataataaa
     3421 tcccattatt aatgtaggca ataatgtcct gaatattatt ggtactgtaa tggtgaaaaa
     3481 gactcttacg ctaccagcac cttctatcat agctgcttca taatagtcct ttggtataga
     3541 ttttaaaaaa cctaaagcta aaaacatata gtatggggta cccatccatg atacaacaaa
     3601 tatagttgaa agtctggcta gatatggatt atcaagccac ataaccttac caaatcctaa
     3661 tatattaagc aggttattta aagctccccc atcagttgct agaatccctt gccatgttaa
     3721 aagcattata actgatggaa tcacccaggg caggataaat atcattttgt aaattttagc
     3781 gatagtggca tttctttttt ccagtaatgt agccaacaag gttcctaata taaaagttga
     3841 tgtcataacg caagcagcga aaataatcgt ccataagaac attgctacaa aaccattaag
     3901 tccctcaata aaaatcgttt tataattata tccaccaaca aatacataat ccactagatt
     3961 caaagaacta taatttgtca tcgaaagata tatattatgt actataggaa acacataaaa
     4021 gattcctatc agccctataa tcggaagcag tacaacataa gaaatagtag ttgctctaag
     4081 cattatcctg ttagctgatg cactgaattt ttttcgtttt ctaataatag ttattactaa
     4141 tataccaaga cctataacta caaaactacc taagaatata tacccccaaa gagaaatatc
     4201 cactttttct acctgtacat tcatttttcg cacatcattt aatatagctg atgctagttc
     4261 gtctagcata ttatctgaat ctaatggatt tccatttata tctccataaa aaattgactg
     4321 atatgcttta tcagtatagt tgtaccatat atctccttca ggtatattag ggacaggttc
     4381 aacattttta catatctgag ccatcacatt atacatgggt tcgcttgtaa tgtatttgct
     4441 ttgctgaact gataaattga caggaaaacg acttcctatt tttctctcaa aattattacc
     4501 agcttctatc attttttgct gattttcatc cttgcataag aattccagaa aagcaatagc
     4561 ttcatcagga aaattggtgt gagcattaat aacaaaacca tttattgttg catatggagc
     4621 tgatgtagta ttatcattat tctttggtag aggtgcaaat ccaaaattta cgtctttttg
     4681 cgtataaaag gaattctgcc agaaaccatt gatgatcata gcagattgat tattagcaaa
     4741 tgctgctgaa acatcattat ctgtacagtt tctacttcta gcaactagat caaattgcat
     4801 aagttctttt attttattga cataaatttt agaaccttca ttattaagac cgatatcata
     4861 tggattataa ttgccttctt catcaaatcc gtaataataa ccaccataat ttctgattat
     4921 aggatacata aaatacatgt tcttcggatt taaaataaat gccttcattt gagaaccttt
     4981 atgttcaatt gttccgaagt tttttgagaa ttcaaaaaat tcatcccatg tcaaatcaga
     5041 aatctgatcg taggatatta catctttatt ataaacaaga ccatatgtct caactgaata
     5101 cccaataccg aattgtttat cattaacctc aaatgaagta aatgcagatt catcaaaagc
     5161 tgacttaatg ctctccgaaa aatccaattc cattaaaaat ccattaacag caaggtctcc
     5221 tatatctgca tgttgaatca taactatatc aggtctttcc tgagtttggg cattgttaat
     5281 aaggtctttt ggtgcatcaa acaaacttct tctatcaact attagaacat cagtaccagt
     5341 cttttcttta taactttctg caatatttct caagacatca ttttctctag tgatctcact
     5401 tccccaaact cgaattactt tatttgtctg agcattaaca ggataaatga agaaaaaaga
     5461 tatcagtgtt aatattatag ttagtaggca tatgactcta tttttcaaag cattctccta
     5521 attacaaact acgaaacttt tctgtgtaac taattgcttc ctctatcgtt cttaccggaa
     5581 gacagacaga gaaatttccc tttttcaaga tatcaatatt gctttctatt tcttcaaatg
     5641 agttacaatc tattgctaga caagttccat tagcatcttc aatcaactga tcaagcatat
     5701 caaaaggcct tggatgatta ggatcagttg ctaaccagag atttgtaaca tttttcagat
     5761 cacatatatg ctttacaaga tatattgcac aactatgact atgcatatgc ccacttccaa
     5821 aatgactaat aattttttga gtatgaggag cacagaattc atcatataaa tccggactta
     5881 tcatgctggc tatatcctct gacatattaa taattccatc aatgaacatc gttccatatg
     5941 tcggattttt cctatattta cgtatcaatc catatatatc ttcagcaaat tttatagtaa
     6001 catcagcaca ataatccaat aattcatgga gtttatcagg attatcataa aaatcagtat
     6061 atatctcaga cccatgcaat gcttctgcaa gatcaagagg tgaaaaaaag cctcttgcaa
     6121 aaggaatatc cgtatctttt gtcttactta ataccctctc taatatgtac atatatttcc
     6181 tataccactt agatgtacca attggaggta aatccttgta cattttcaga tcagtaataa
     6241 taggcaatga ccagcttgtg tccttcttaa aataaacatc acctgctaca aatgctgcat
     6301 aatctcctat tcctaaattg atattgagtt gcgggatata gttgtcttct acactttttc
     6361 ttgcttccat catagagaaa tgacctttta aaagattatc tacataaatg tccaagtctg
     6421 tttcgttgta tttgtatgct gcaagattaa gatactcttt aatatcacta tctggtaaag
     6481 cacggaatgt aacatttcca tttatatgat taagtgcatc agaatatgtc ttactcattt
     6541 cagatgctat cttggcaata cttggtttta gctgggttat atgtttcatc gcattgcttc
     6601 tttctttatg attaaccggt taagcaagat aaacatatca catattaagg tctatgtcaa
     6661 tacctttatc aactcggatc tatcaagaat atatgttgct ctttttctac tgcttgacgc
     6721 gtatctaatt ttttaaatat tatcttaatg tagtccttaa gtacctgact gtcaaataca
     6781 acctgtttta cctcgacagg aaaagctaca ttctcagaaa aagtaattag aatcaactgg
     6841 ttatatctta tagcagatag tgcaataaac ccaggaatgt ctccaatcgc tataaaattc
     6901 ttctctatgt tatttttaac aaatacatca atgtcttttc tgttttctcc tgtgaaagct
     6961 atctttttca gtggaatatc ttcaattttt tcgtttattc ctttattatt tcttaaacta
     7021 taaattaaaa cacattcttt ctcgttatca aataaggtaa tcgccttctc aactgccttt
     7081 tgataattat ttattaattt gaaaaaaaga ttatctgaga acatactatc aaaaagtatc
     7141 ataggagaat atgtatttat gccaatattt cttatctgct cttcactaat atttattgca
     7201 aggattaaat catatgctaa atccatataa ttatcctgac gcttattatc taggagaact
     7261 aatttataat catctgcatg aaaaatttca tagagcatat atataatttt acattcgctt
     7321 ataaaacttt tgcatgaact gtctccaata attataccta taagtttctt tctgttatca
     7381 caaagatact tagcagataa atttggaaca tactgataga gatttgctat ctgtaaaatc
     7441 cttcgtcttg tctcgtcact tattctagca tttttcttat catttaggac atatgaaact
     7501 gtggcttttg agacattagc agctttagct atatcctgca tcgtatactt ttttaccatg
     7561 ttatcctctt caaggttgtc caataaatat ttctaattta atatatcaca caaaaaatat
     7621 tatgtaaatt tatattaaaa tataaaagac ttctgtatcg acccaacagc caaatatttg
     7681 aatatagata ttaaattgat aataacatat tatatagcta tactgttata tatactataa
     7741 atgcaaggag atacaaatga aattatcaca gacaaacggc ccatccttta acttttattc
     7801 tacacatgca gaaacaatca gccatttaaa caaatgcgga ttcagacatg ttgacatatc
     7861 at
//
LOCUS       DAOSXM010000095         7541 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2111
            NODE_6301_length_7541_cov_12.529789, whole genome shotgun sequence.
ACCESSION   DAOSXM010000095 DAOSXM010000000
VERSION     DAOSXM010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864178
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 7541)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7541)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:24:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,261
            CDSs (total)                      :: 2,229
            Genes (coding)                    :: 2,218
            CDSs (with protein)               :: 2,218
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 3 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7541
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6301_length_7541_cov_12.529789"
                     /isolate="AMR_MDS_2111"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..798)
                     /locus_tag="PLQ68_09620"
     CDS             complement(1..798)
                     /locus_tag="PLQ68_09620"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HXK72952.1"
                     /translation="MIIQSIRHEWPEKAGFILDRPHGPEEFIFIHYFNSVDLLSNNEV
                     IRTKPHACIVVSANKPQWFKSEQKLVHDWFHFTKDTLNSKITNESIPVIMNTYGIEPD
                     KVYYPSDSLEITNTVRKMESEFFTDVGFSKELCDLYIRQIFILMARSSKDNLSGISIK
                     KNLLHKVEQVRRQMFFFPMEDWPVSKMAKMAGLSESRFYTVYKGVYKITPNRDLIFAR
                     IEKAKNLLSSQGYSVNEAAEALGYNSVYHFIRQFKAVTGSTPGEFKI"
     gene            925..2628
                     /locus_tag="PLQ68_09625"
     CDS             925..2628
                     /locus_tag="PLQ68_09625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015736857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HXK72953.1"
                     /translation="MTKIPRPEHPRPQMYRESWINLNGEWQFEIDHGKSGRDRCLYKN
                     TELNSSIIVPFCPESELSGINNKDFMECVWYKRKVDIKNDWLKDGRRTILHIDACDYH
                     TEVWINGDSVGTHIGGYSSFSFDITEKLIKGDNTVTVCATDLLRTGEQPGGKQCREFF
                     SRGCSYTRTTGIWQTVWLENVASSSIKSFKCTPDIYSSSIYFDVLCRNANGKKVKATA
                     YFAGKEVGSCLAYIEGKHARFNMELKELHLWSCETPNLYDLVLELDNDKMSGYFGMRS
                     THYHGNKFYLNNQVVFQRLILDQGFYPDGIYTAPTDQELINDIKRSMDMGFNGARLHQ
                     KVFEPRFLYHCDVLGYLVWEEHANWELNISKDKAWERFVPEWLEIMERDYNHPSIIGW
                     CAFNETQKDQNVNLVKYVADMTRAFDSTRPVIDTSGWVHIDGASDLRDKHDYDQNPVT
                     FKERYDQIDQQKKNYDGYVDDFCAPVFISEYGGIWWSETDTSGWGYGQRPNSLDEVIG
                     RYRGLTEVLLNNPNIGAFCYTQLTDVEQEQNGLYTYDRKPKMDPAIIKEINKQKAAVE
                     K"
     gene            complement(2747..3568)
                     /locus_tag="PLQ68_09630"
     CDS             complement(2747..3568)
                     /locus_tag="PLQ68_09630"
                     /inference="COORDINATES: protein motif:HMM:NF014679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HXK72954.1"
                     /translation="MIRIITDSTSYFKQAEAEEYGIKVLPITYKSGNYIYLESYSGTD
                     ADIDNLLRSGYGYSTSHPDEAVFSSTFQNEIMQGNQVLCIPISSRLSLAYKAAMDAAV
                     KLDNSNIAVFDSLQTAGGLHLLVKYAKLMANDGISLSKIYMALPKLNSKISTFFSVND
                     MISLRRSGRIGIVRMNVSTIMNLRPILSLKDGTIVSEDMARGTMDVIRKLSDKMPDNT
                     KEAVINYIENTDTAIGVYNALKTKAPYLPIKLRKSGPVLAIHVGLGFVSVSYITE"
     gene            complement(3561..4331)
                     /gene="accA"
                     /locus_tag="PLQ68_09635"
     CDS             complement(3561..4331)
                     /gene="accA"
                     /locus_tag="PLQ68_09635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014356290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HXK72955.1"
                     /translation="MNAYDHVLIARSKGRNTCIDFIKEIFTDFTEMHGDRRFKDDMAV
                     ICGLAYLSDIPCTVIGLEKGKDNNDKQKRNFGSAHPEGYRKALRHMKLAEKFNRPIIM
                     FVDTPGAFCGIEAEQRGQGQAIAENLYEMSAIKTPMLSVLIGEGGSGGALALAVADEV
                     WMLENAVYSVISPEGCASILWKDSSKVKQASENLKLTADDLKSLGVVERIIEEDETIF
                     KNLHDDMYELIRKNLKLDKDELVLKRYTRYRRMGNLND"
     gene            complement(4328..5191)
                     /locus_tag="PLQ68_09640"
     CDS             complement(4328..5191)
                     /locus_tag="PLQ68_09640"
                     /EC_number="2.1.3.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013280664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HXK72956.1"
                     /translation="MLFKRPKNSLEGNPKIQLKLFKDKSDTTDKEMFTCKKCKKTYSK
                     NELLESLNVCPFCNEHYKISARERLNILCDSVSFTEHYSELVSCNILGFEGYDEKLAE
                     ARKQSKENESVVCGVCTIEGIKTAIFVMDPFFMMGSMGSVTGEKITRLFEYATDNSLP
                     VVGYCVSGGARMQEGILSLMQMAKVSAAVKRHSDMNNLYLCVLTDPTSGGVTASFAMQ
                     ADIILSEPKALVCFAGPRVIEQTIRKKVPDDIQRAECVMENGFIDNIVGRKSQKSYIG
                     RLLKMHMRYEV"
     gene            complement(5205..6542)
                     /gene="accC"
                     /locus_tag="PLQ68_09645"
     CDS             complement(5205..6542)
                     /gene="accC"
                     /locus_tag="PLQ68_09645"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015924457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HXK72957.1"
                     /translation="MFSKILIANRGEIAIRIIRACKEMGIYSVAVFSEADKNSLHVSL
                     ADEAYCIGKAQVKDSYLNMHAIISAALASKAEAIHPGYGFLSENYEFASLCEKYNIAF
                     IGPDAKTMTNMSDKEKAIQIMKEAGLPIIPSSKVVVNVEQAVLEAEKIGYPLLIKARS
                     GGGGKGIRLARNVEELKYGFHVATSEAASAFGDDGVYLEKFLSSVKHIEMQIIADNHK
                     NAVCLGERDCSTQRKNQKMIEESPCAALPENIRKKMSESSIRAVKATGYKGVGTLEFL
                     YDGKDYYFMEMNTRLQVEHPVTEMVTGIDLVKWQIRVASGMKLGFTQEDISPSGHAIE
                     CRINAEDAYTGFRPSCGKIETLHIPGGPWVRFDTAIYQGYVIQPFYDSMIGKLIVHAK
                     TRDEAIRKMKAALCELIVEGIDHNARYNEEILDTDEFVSGKYTTDYLSALEMI"
     gene            complement(6545..6958)
                     /locus_tag="PLQ68_09650"
     CDS             complement(6545..6958)
                     /locus_tag="PLQ68_09650"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015522656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ family
                     protein"
                     /protein_id="HXK72958.1"
                     /translation="MEREEIKKILPHREPMLLVDRVEIKDDICIGEYTVTGEEFFLKG
                     HFPDDPVVPGVMLCEMMGQSSCLLIEADGEFIPYFTKMDNVKFKKPVKPNDKIIFKSH
                     ITNIRKPFYFITCEGYVNDVLCVSGDLSFAIMKKE"
     gene            complement(6961..7410)
                     /locus_tag="PLQ68_09655"
     CDS             complement(6961..7410)
                     /locus_tag="PLQ68_09655"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, biotin carboxyl carrier
                     protein"
                     /protein_id="HXK72959.1"
                     /translation="MNNKDVRALAKIMNEYGLSFIEWKDENSSITLKKDCLTGETVSK
                     SSKDEIEKVHNNVGNIADDISDKEDFNDVIEVKSPFVGVFYSTASPDNLPFVSIGSEV
                     KEGDVMCIVESMKIMNEIIAPKDGKVIDICVKNKDIVEFGQVLYKLI"
BASE COUNT         2241 a         1563 c         1236 g         2501 t
ORIGIN      
        1 ctatatcttg aattctcctg gcgtagaacc agttacagct ttgaactggc gtatgaaatg
       61 atacacactg ttatacccaa gcgcttctgc tgcttcatta acagagtatc cctgacttga
      121 cagcagattt tttgcttttt caatacgtgc gaatataaga tccctgttag gcgttatctt
      181 gtaaacacct ttgtaaacag tataaaaacg tgactcactc agtccagcca ttttagccat
      241 tttcgatacc ggccagtctt ccatcgggaa aaaaaacatc tgtcgtctta cctgctcaac
      301 tttatgcaaa agattctttt taatacttat ccctgacaga ttgtcttttg atgatctggc
      361 catcaatatg aatatctgac gtatgtataa atcacatagt tctttggaaa acccgacatc
      421 tgtaaaaaac tccgactcca tttttctcac tgtatttgta atctctaagc tgtcggaagg
      481 ataatacact ttgtccggtt ctataccata tgtattcata atcacaggaa tactttcatt
      541 ggtgattttg ctgtttaaag tatcttttgt aaaatgaaac cagtcatgaa caagcttctg
      601 ttccgatttg aaccactgtg gcttatttgc agaaaccaca atacatgcat gaggttttgt
      661 tctaataact tcattattgg ataataagtc aaccgaattg aagtaatgaa taaatataaa
      721 ttcctctggt ccatgtggcc tatccagtat aaatcctgct ttttccggcc attcatgcct
      781 aatactttga attatcataa aattaccttt caatatatat aatatcaata cagtaggata
      841 tgtcaacttt atcaagtatt atgcattgaa tcagtctgtt atcagttata aactgaaaac
      901 atattataca aggagcaaat aaaaatgact aaaatacctc gtcctgaaca cccaagaccc
      961 cagatgtaca gagaatcatg gattaattta aacggagaat ggcaatttga aatcgaccac
     1021 ggtaaaagcg gaagagacag atgcctttat aaaaatacag aacttaactc ctccatcata
     1081 gttcctttct gtcctgaaag cgaattgtcc ggaatcaata acaaggattt catggaatgc
     1141 gtatggtata aacgtaaagt tgatataaaa aacgactggt tgaaggatgg aagaagaacc
     1201 atacttcata tagatgcatg cgattatcat acagaagtct ggattaatgg tgattcagtc
     1261 ggaactcata tcggtggata tagctcattt agctttgata taacagaaaa acttataaaa
     1321 ggagataata cagtaactgt atgtgcaaca gatctattaa gaactggtga gcagcccggc
     1381 ggaaaacaat gcagagagtt tttctccaga gggtgcagtt acacaagaac tactggaata
     1441 tggcagactg tatggctgga gaatgttgca tcttccagta ttaagtcatt caaatgcact
     1501 cctgatatat actcatcaag tatatatttt gatgttttat gcagaaatgc aaatggtaaa
     1561 aaggtaaaag caactgcata tttcgcagga aaagaagtcg gttcctgctt agcatatatt
     1621 gaaggaaaac atgcgcgttt caatatggaa ttaaaagaac ttcatttatg gtcttgtgaa
     1681 acaccaaacc tgtatgattt ggtgcttgaa ctggataacg ataaaatgtc tggttacttt
     1741 ggcatgagaa gcacacatta tcatggaaac aagttttatt taaataatca ggttgttttc
     1801 caaagattaa tccttgacca gggcttttat ccggatggaa tatatactgc tccaactgac
     1861 caagaactga ttaatgatat aaaaaggtca atggatatgg gattcaacgg agcaagactt
     1921 catcagaaag tatttgaacc aagatttctt taccactgtg atgtacttgg ttatcttgta
     1981 tgggaagaac atgcaaactg ggaactgaat atatcaaaag acaaggcctg ggagagattt
     2041 gttcctgaat ggctggagat aatggaaaga gactataatc acccttcaat aataggatgg
     2101 tgtgctttta acgaaactca aaaagaccag aatgtaaatt tagttaagta tgttgccgat
     2161 atgactagag cttttgattc cacaagacca gttatagata cctctgggtg ggttcatata
     2221 gatggagcat ctgatttaag agacaaacat gattacgatc agaaccctgt aaccttcaaa
     2281 gaaagatacg accagataga ccaacagaaa aagaactatg acggttatgt agatgacttc
     2341 tgcgcacctg tattcatttc agaatatggt ggaatatggt ggtcagaaac tgatacatca
     2401 ggctggggat atggacagcg tccaaatagc cttgatgaag ttattggaag atacagagga
     2461 ttaactgaag tgttattaaa taacccaaat atcggtgcat tctgctatac acagcttaca
     2521 gatgttgagc aggaacagaa tggattgtat acatacgaca gaaagcctaa gatggatcct
     2581 gcgataataa aagaaataaa caaacaaaag gcagctgttg agaaataaat tatagccagt
     2641 atgtaaatgt tctgtaagta ctgttgtaaa tatttagaaa aatggtgttg ttcatatgag
     2701 cagcaccgtt ttctaatcat attaactttt tgaacgaact agtatttcat tccgtaatat
     2761 atgacacact gacaaatccc aagcccacat gtattgcaag cactggccct gattttctaa
     2821 gctttattgg aagatacgga gctttggtct ttagcgcatt gtacactcct atcgcagtat
     2881 cagtgttttc tatgtagttt ataactgctt ctttggtatt atcaggcatc ttgtctgaca
     2941 gcttccttat aacatccatt gttcctcttg ccatatcttc tgatactatg gttccatctt
     3001 taagagaaag aatcggcctt aagttcatga tagtacttac attcattcta actatgccaa
     3061 ttcttccact ccttctgagg cttatcatgt cattaactga gaaaaatgtg cttattttac
     3121 tgtttagctt tggaagcgcc atatatatct tacttaatga gattccatca ttagccatta
     3181 atttagcata cttaactaaa agatgaagcc cgcctgctgt ctgaagactg tcaaataccg
     3241 ctatatttga attgtccagt tttactgcag catccattgc tgctttgtat gctaaagaca
     3301 gtcttgatga aattggtata cataatacct gattaccctg cataatctca ttttgaaaag
     3361 tgcttgagaa aacagcttca tcaggatgtg aggtggaata cccgtaaccg cttcttaaaa
     3421 gattatcaat atcagcatcg gttccgctgt atgattcaag gtagatatag ttaccgcttt
     3481 tgtatgttat aggcagcacc tttataccat attcctctgc ttcagcctgt ttgaaatatg
     3541 aggttgagtc ggttattatt ctaatcattt aaattaccca tccttctata cctagtgtat
     3601 ctttttaaaa ccagttcatc tttatctaat ttcaaattct ttcttattaa ctcatacata
     3661 tcatcatgaa gatttttgaa gattgtttca tcttcttcta ttattctctc cactacgcct
     3721 aaagacttaa gatcatcagc agtaagcttc aaattctcac ttgcctgttt tacttttgaa
     3781 gagtctttcc ataatatact ggcacagcct tcaggagata taacagaata tacagcattt
     3841 tccagcatcc agacttcatc ggctactgct aatgctaacg ctcctccaga accgccttct
     3901 ccaattaaca ccgacagcat gggagttttt atagctgaca tctcatacag attttcagca
     3961 atagcctgcc cttgccctct ttgttctgct tcaataccgc agaaagctcc aggagtatct
     4021 acaaacatga taataggcct gttaaatttt tcggctaact tcatatgcct taatgctttt
     4081 ctgtatcctt cagggtgagc agaaccaaaa tttcttttct gcttatcatt attgtccttg
     4141 cccttttcca gccctataac cgtacatggt atatctgata aatatgccag tccgcatata
     4201 actgccatgt catctttaaa tcttctgtct ccatgcattt ctgtaaaatc agtaaatatt
     4261 tcctttatga aatcaataca agtatttctt ccttttgaac gtgcaataag tacatggtca
     4321 tatgcgttca tacttcatac ctcatatgca tctttaaaag tcttcctata tagcttttct
     4381 ggctttttct tcctactata ttatcaataa acccgttttc cattacgcat tctgctcttt
     4441 gaatatcatc aggaaccttt ttccttattg tctgctcaat tactcggggc ccagcaaaac
     4501 agacaagagc tttaggttca gataatatta tgtctgcctg catagcaaaa ctagccgtaa
     4561 cccctccact tgtagggtca gtcaatacgc atagatatag gttattcata tcagaatgtc
     4621 tctttacagc agcacttact tttgccatct gcatcaaaga aagtattccc tcctgcattc
     4681 ttgctccgcc tgacacacag tacccgacca caggcaaact attgtctgtt gcatattcaa
     4741 aaagccttgt tatcttctca cctgttacgc ttcccatact tcccatcata aaaaaaggat
     4801 ccataacgaa tatagctgtc tttattcctt ctatagtaca gactccgcaa accactgatt
     4861 cattctcttt actttgtttt cttgcctcag caagtttctc atcataacct tcaaatccta
     4921 atatattaca agagacaagt tcactataat gctcagtaaa tgagacagaa tcacataata
     4981 tattcaatct ttcccttgcg gatattttat agtgctcatt gcaaaagggg catacattta
     5041 gtgattccag tagttcattt ttagagtatg tcttcttgca ttttttacaa gtgaacattt
     5101 ccttgtcagt tgtgtctgat ttatctttaa aaagctttaa ttggattttg ggatttccct
     5161 caagggaatt tttaggtctt ttaaacagca tttttaatcc tttcctaaat catctcaagc
     5221 gcgcttaagt aatctgtagt atattttcca gatacgaatt catcggtatc aagtatctct
     5281 tcattgtatc ttgcattatg gtctatacct tcgactataa gctcgcaaag agcagctttc
     5341 atcttcctta tagcttcgtc ccgggtttta gcatgcacaa tcagttttcc tatcatggaa
     5401 tcataaaaag gctgtatgac atacccttga taaatagctg tatcaaaacg gacccatgga
     5461 cctccaggaa tatgaagagt ctcaattttc ccgcaggaag ggcgaaatcc tgtatatgcg
     5521 tcttcagcat ttatcctgca ttcgatagca tgacctgaag gtgatatgtc ctcctgtgta
     5581 aacccaagtt tcattcctga ggccaccctt atctgccatt tgaccagatc aatccctgtt
     5641 accatttccg taactggatg ctctacctgg agccttgtat tcatctccat aaaatagtag
     5701 tctttaccat catacagaaa ctctagtgtt ccgacacctt tgtatcctgt ggccttaaca
     5761 gcccttatag atgactctga cattttcttg cgtatatttt caggtaatgc ggcacagggg
     5821 ctctcctcta tcattttttg attttttctt tgtgtggaac agtctctttc cccaagacac
     5881 actgcatttt tgtgattatc ggcaattatc tgcatttcaa tgtgttttac agaagacagg
     5941 aatttttcca aatatacccc gtcatcacca aatgctgatg cagcttcaga tgtagcaaca
     6001 tggaatccgt atttaagctc ttcaacattt ctagcaagcc ttataccttt cccgccgcct
     6061 ccggaacgtg cctttattaa cagcggataa cctatttttt ctgcttccaa taccgcctgc
     6121 tcaacattta ctacaacttt gcttgacggt attatcggaa gacctgcttc tttcattatc
     6181 tgaattgctt tttccttatc agacatattg gtcatggtct ttgcatcagg tccaataaat
     6241 gctatattat atttttcaca caagcttgca aattcataat tttctgacaa gaaaccatag
     6301 cctggatgaa ttgcttctgc tttggaagca agagctgcag aaattatggc atgcatgttt
     6361 aaataactat cttttacctg cgcttttcct atacagtatg cttcatctgc aaggctgaca
     6421 tgcaaagaat tcttgtctgc ttcagaaaaa acggcgacac tatatatgcc catttccttg
     6481 cacgccctta ttatccttat agcaatctct cctctgtttg caatgagtat ctttgagaac
     6541 atgtctactc ctttttcatt atggcaaaag acaaatcgcc gcttacacat aaaacatcat
     6601 tcacataccc ttcacaggtt atgaagtaga agggttttct tatatttgtt atatgacttt
     6661 taaatattat cttatcatta ggttttacag gttttttaaa cttaacatta tccatctttg
     6721 tgaaatatgg tatgaattca ccatcagctt caattaacag acagcttgac tgacccatca
     6781 tttcacaaag catgactcct ggaactacag ggtcatctgg aaaatgtcct tttaagaaaa
     6841 attcttctcc tgtaactgtg tattcaccta tgcatatatc gtcttttatc tcaacacggt
     6901 ctactaaaag cataggttct ctgtgtggaa gtattttttt gatctcttct ctttccatat
     6961 ctatatcagc ttatacagaa cctgtccgaa ctcgactatg tccttgttct taacacagat
     7021 atctataacc tttccgtctt ttggagcgat aatctcattc attatcttca tggattcaac
     7081 tatacacatt acatcgcctt ctttaacttc acttcctata gagacaaatg gaaggttatc
     7141 cggtgatgct gtagagtaga aaacccctac aaaaggtgat ttcacctcaa ttacatcgtt
     7201 aaagtcttcc ttgtcagaaa tatcatctgc tatgtttcca acattattgt gaaccttctc
     7261 tatctcatct ttcgagcttt tacttacagt ttcacctgtc aaacagtcct ttttcaatgt
     7321 tatcgaacta ttttcatctt tccactctat gaagctcaat ccatattcat tcattatctt
     7381 ggccagtgct ctgacatctt tattgttcat agtaagtcac ctcatattct gttaaatata
     7441 atgccggcat tgtgtcctcc gaaccctaag gaaaaggaca ttgctgtatc tatatcatat
     7501 tctcttgctt cgtttggaac atagtccaaa tcacagtctt c
//
LOCUS       DAOSXM010000096         7472 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2111
            NODE_6392_length_7472_cov_10.506674, whole genome shotgun sequence.
ACCESSION   DAOSXM010000096 DAOSXM010000000
VERSION     DAOSXM010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864178
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 7472)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7472)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:24:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,261
            CDSs (total)                      :: 2,229
            Genes (coding)                    :: 2,218
            CDSs (with protein)               :: 2,218
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 3 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7472
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6392_length_7472_cov_10.506674"
                     /isolate="AMR_MDS_2111"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(121..1170)
                     /locus_tag="PLQ68_09660"
     CDS             complement(121..1170)
                     /locus_tag="PLQ68_09660"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HXK72960.1"
                     /translation="MDELFDLNKYLIPIWEGDTVYDESVMVIEDKDGFIPLQSLAYEA
                     LSILSVKNAELTKSYNEGVDYALKDGKLEILNNSSIYHMSYDEYYPKEDLSGKCFGTD
                     NGFTTWQEEGFLHKRQIAVTYTHKPNDIYQPIRKNRKTDILSRLNNKENLKFLFYGDS
                     ITFGWNCSKLADLSPNLPSWGELTVKGLSAKFGYNDTSIEYINTSVSGTSVNWGLENV
                     KQRVCEYNPDMVFIAFGMNNPKTDPDIFKDTLEKMITKIRNTSPLCNIGLVATTLPNP
                     EIVSTRGHRHLYLPLLWQIAENFDNIVVCDMTTLHTKLLAKKPYFHMTGNNVNHPNDF
                     LMRQYAAMALDALIH"
     gene            complement(1276..2640)
                     /locus_tag="PLQ68_09665"
     CDS             complement(1276..2640)
                     /locus_tag="PLQ68_09665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008712965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HXK72961.1"
                     /translation="MTTTPVPKLIITLAIPMILSMLVSAIYNLADTYFVSQLGTSASG
                     AVGVVFSLMATIQAVGFMLGMGTGAQISRLLGQKKNSQANVVAINGLVSAIIFGILML
                     VLGLKYHEQILLILGSTETILPHAEAYAQYIFYAAPFMAGSFVLNNILRSQGKAKFAM
                     IGIMTGAVLNIGLDPLFIFGFDMGIGGAALATAISQTIGFMVLLVPFFKKMTEVRLSF
                     KFFSFKPIVLLRIMKNGLPSFIRQGFASAATVILNRQASLYGDSAIAAMSIVLRIVMF
                     IFIIVIGFGQGYQPVVGYNYGAKIYDRVKEAFVFSLKVGMLIMAFFAIIGFVFAPGLM
                     AVFLPEDAAVIEIGALAFRAQCIAMLFMPFSTLANMTFQSIGKSWTATFLSSLRQGIF
                     FLPLIIFLPRFIGLAGVQFTQAISDVLTFLVCLPFVISFFVKLKKRDLYQPKDEIQLE
                     IVEG"
     gene            complement(2747..4555)
                     /locus_tag="PLQ68_09670"
     CDS             complement(2747..4555)
                     /locus_tag="PLQ68_09670"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXK72962.1"
                     /translation="MKASKKISLVVSLVWKLKPVYFITLFLNSLAFSGHILTNVIFPK
                     YLIEELMGNQDRTMLISWILVIVLGNAFFTLTKKTLKRLLDVQEKDLFWQIERAFSEK
                     IMSIEYRYLEDPYYLDLKERATFAMRNQSSLSRLINTAVTFINQVVTVLGLIVLMLNL
                     SWVLVVSLLFTVFLTLVIQAMFSKYQLKFFSELLPVNRRYGYYIQLTFDKLIHKDSRI
                     FNMTDMLRDIIIKYNLQINKWFGKFYRRMGLYLGLFQVVVVLQTVLAYGYVGARTLAG
                     DITIGDLTMYVSAAVGFSSAVIALGEAIIDIMQVLGYLEPFVELMEIPLAENDKGSES
                     IDIIESIEFENVSFKYPKTDRLILDDISFVIEKGQKISIVGLNGAGKSTIVKLICRLY
                     KPDSGKILINKKDIHDYSYKEYLSCISAVFQDYKLFNFSIQENISCKDISEDDPKVKN
                     ILDMVSMSKKIESLPNGVKSLFGKEYDEEGVEFSIGQSQKIAIARALYKGSGFVILDE
                     PTSALDPIAEAEIYENFNDLVGDKTAIYISHRMSSSVFCDKILVINEGKIEAFDTHKN
                     LLKDENSLYSKLFTSQAVNYRYEKVMKTYSAVNDIV"
     gene            complement(4552..6345)
                     /locus_tag="PLQ68_09675"
     CDS             complement(4552..6345)
                     /locus_tag="PLQ68_09675"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXK72963.1"
                     /translation="MSKKNNSALKRMLGYAWVEDKSIYFLCAIYTLAAIMVPVISVAL
                     PKVIIGYLTEGEALVSGIVRLALIFFISGATVYFLKEWLLDYTYPRITTLRIDYIKEQ
                     AVKLLTMDYKYMEQAEFFNSRERAFESTSSNNNGVEGIFHKLFELPQLVLIVLALSVF
                     IGIKSIWILLALILHIFVTTYIAIIVQKYQYKRKEELSKKERRVSYYSSTSKDFSFGK
                     DIRMYDLKTRILDNFKKEISGYIDIFKVIKNKEYLLGFLSLLTLIIADIAAYGMLTIL
                     VVKGMSIADYSMYIAACVTLTVQMTSLSENVTYIMQEYMYVKDLYKFFDEDLGNKGGT
                     LPAVSKDMPLEIEFIDVNFTYPGTDKPIFTNFSLHIPPASKLALVGINGAGKTTFVKL
                     LTGMFKADSGKILINGKDINEYDRQELFSMFCVVFQEVNILALTIAQNIACSIDNIDY
                     ERVDEVLKQVDLYDKVKSLDKGVDSMMLKVIEEDGVVFSGGENQRLAIARALYKGGNC
                     VIMDEPTASLDALAEASIYSQFDTLIHGRTAIYISHRLASTKFCDSIALIDNDGLSEY
                     GTHDELMALGGAYHDMFIVQGKYYQTQEEEI"
     gene            complement(6329..>7472)
                     /locus_tag="PLQ68_09680"
     CDS             complement(6329..>7472)
                     /locus_tag="PLQ68_09680"
                     /inference="COORDINATES: protein motif:HMM:NF024835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HXK72964.1"
                     /translation="YIRYLLLKIMVLTVKDGVMELHLNENIKKYRKKRGMTQEQLSEA
                     MGVSVGAVSKWESGQMNPEIGMIVRIADFFELSVDSLLGYSYRSRNLSDTLNLIKSLS
                     NKKDYSAVKDEIFKALLKYPNDFDIVYQSGITLSNLGWEREDKEAVAQSLVLLEHSCS
                     LIEQNSNPDISIGSIKSRIAIILSSEKRYDEAIDIFKEYNVNGVNNSWIGKSYTEMKK
                     YDQALIAFSESYIDNLVNIYICITGMCTCFVEMGRHSEALEMIIWLKNTLNALEYKDR
                     PSYLIKMGMMLDTIQVMLRYAMKDKDSVVLLMKDIAKKARLFDSNPDFSCNSIRFYSG
                     KEHFFCDDIGKSAVEAVEKLLNMNDSKEDRDNLVAKWKEVLSEQKE"
BASE COUNT         2408 a         1504 c         1063 g         2497 t
ORIGIN      
        1 ctcaacgctt accccgcatt gacgcactgg tattcttcaa agactgctgc ttgtctttct
       61 atttttcctt ctttattaag ttaataaatt atttttccca aaactcttta cactaaccta
      121 tcaatggatg agtgcgtcca gtgccatagc tgcatattgt ctcataagaa aatcgttagg
      181 atggttcaca ttattaccag tcatatggaa ataaggtttt tttgctaaca gttttgtatg
      241 caacgtggtc atatcgcata cgacaatatt atcaaaattt tctgctatct gccataacaa
      301 aggcagataa agatgcctgt gtcctcttgt tgaaacaatt tcaggatttg gcagagttgt
      361 tgcaactaga cctatattac acaaaggact ggtgtttcta atctttgtaa tcattttttc
      421 taatgtatct ttgaatatat caggatctgt ctttggatta ttcataccaa atgcaatgaa
      481 taccatatca ggattatact cacacactct ttgttttaca ttttccaatc cccaattaac
      541 agaggttcct gatacagatg tatttatgta ttctatagaa gtgtcattat atccaaactt
      601 agctgataat ccctttacag taagctctcc ccatgaaggt aggtttggag ataaatcagc
      661 gagttttgaa cagttccatc cgaatgttat actgtctcca taaaacagaa attttaaatt
      721 ttctttatta ttaagcctgc ttaaaatatc agtctttcta ttttttctta taggctgata
      781 tatatcatta ggtttatgtg tatatgttac agctatctgt cttttgtgta aaaacccttc
      841 ttcctgccat gtagtaaaac cattatcagt tccaaagcac tttccagata aatcctcttt
      901 cggataatac tcatcataac tcatatggta aatcgagctg ttattcagta tttccagctt
      961 tccatcctta agagcgtaat ccacaccttc attatagctt ttagttagct cagcattttt
     1021 tacagacagt attgataatg cctcatatgc caagctctgt aaaggaataa atccatcctt
     1081 atcttcaata accattacag attcatcata tacagtatca ccttcccata taggtattag
     1141 atacttattc aaatcaaaaa gctcatccat aattgtcctc gtatgataca gcaattacct
     1201 gttcatactc ctcctaaata cattttaata ctagtttata gaaaagataa ccatataatt
     1261 tagattattt atctattaac cttccacaat ctctaactgt atttcatcct taggctgata
     1321 taaatctctt ttctttaatt tgacgaagaa agatattaca aatggaagac agaccaggaa
     1381 tgtcaataca tctgatattg cctgagtaaa ttgaaccccg gcaagaccaa taaatcttgg
     1441 aaggaatata atcaatggca agaagaatat tccttgtcgt aatgaagata agaatgttgc
     1501 agtccatgat ttgcctatag actggaatgt catatttgcc aaagtggaaa aaggcatgaa
     1561 aagcatagct atacactgag ctcgaaaagc aagcgcccct atttcaataa ctgcagcatc
     1621 ctcaggaaga aaaactgcca ttaagccggg agcaaataca aagcctatta ttgcaaaaaa
     1681 ggccatgata agcattccaa ccttaagcga gaaaacaaat gcttctttaa ccctgtcata
     1741 tatctttgca ccatagttat atcccacaac aggctgataa ccctgcccga aacctataac
     1801 tataatgaag atgaacatta caatccttaa tacgatagac atagcagcta tagcagaatc
     1861 tccatacaaa gaagcttgtc tgttaagtat gacagtcgca gcacttgcaa atccctgtcg
     1921 tataaaagat ggaagaccat tcttcataat ccttagaagt actattggct tgaaactgaa
     1981 aaatttgaaa cttaatctga cttcggtcat ctttttgaaa aatggaacaa gaagaaccat
     2041 aaatcctata gtctggctga tagcagtagc cagagcagct cctccgattc ccatatcaaa
     2101 tccgaatatg aataatgggt ctaagccaat atttaacaca gctcccgtca taataccaat
     2161 catagcaaat ttggcttttc cctgagacct taaaatatta ttcaatacaa aagaaccagc
     2221 cataaaaggc gcagcataaa atatatactg agcataagcc tctgcatgag gaagtattgt
     2281 ttctgtggaa ccaagaatta ataatatctg ctcatggtat ttcaaaccaa gtacaagcat
     2341 aaggataccg aatattattg cagatacaag accgttaatt gccactacat tggcctggct
     2401 attctttttc tgtcctaaaa gccttgatat ctgagctccg gtacccattc ctaacatgaa
     2461 accgacagcc tgtatagttg ccatcagtga aaatacaacc ccaacagcac ctgatgcgga
     2521 agtacccaac tgtgacacga agtatgtatc agctaaatta tatattgctg aaacaagcat
     2581 acttaaaatc attggtatag ccagagttat tattaacttt ggaactggtg tggttgtcat
     2641 cttgatgtat tgctgctgat gcttatccat agagtatata ttattagatt aaataataaa
     2701 aaacaacata gtatttaaaa ttaatctata ttgtcctcat ttagggctaa acaatgtcat
     2761 taacagccga atatgttttc attacttttt catatctgta gttaactgcc tgagatgtga
     2821 aaagctttga atataagctg ttttcatctt ttaaaagatt cttgtgagta tcaaatgctt
     2881 caatctttcc ctcatttata accaatatct tgtcacagaa aacactggat gacatcctgt
     2941 gcgatatata tatagctgtc ttatctccaa caagatcatt gaagttttca tatatttcag
     3001 cttctgcaat cgggtctaaa gcagatgtgg gctcatcaag tattacaaaa ccggaacctt
     3061 tatacaaagc tctggcaata gcaatcttct gagactgacc tatggaaaat tcaacaccct
     3121 cttcatcata ttctttacca aatagagatt ttactccatt aggaaggctt tctatcttct
     3181 tactcatact caccatatcc agaatatttt taactttcgg atcatcttct gatatgtcct
     3241 tacatgatat gttttcctgt atgctgaagt taaaaagctt gtaatcctga aatactgctg
     3301 aaatacagga cagatattcc ttgtaggaat agtcatgtat atcttttttg tttataagta
     3361 tctttcctga atctggttta tacaacctgc atatgagttt tactatagtt gatttacctg
     3421 ctccatttag tcctacaatg cttatcttct ggcctttttc tataacaaaa gatatatcat
     3481 caagtattaa tctgtctgtc ttagggtatt tgaatgatac gttttcaaat tctatacttt
     3541 caattatatc tatactctca ctgcctttat cattttcagc caaaggtatc tccattagtt
     3601 cgacgaaagg ctcaagatat cctaaaacct gcataatatc tataatagct tctccaagcg
     3661 ctattacagc agatgagaac cccacagcag cagaaacata catagtaagg tcacctatag
     3721 ttatatctcc tgccagagtt cttgctccaa catacccata tgctaaaact gtctgaagca
     3781 ctactacaac ctggaaaagt ccaagataca gccccattct tctataaaac ttgccgaacc
     3841 acttattgat ctgcagattg tacttaataa taatatctct taacatatca gtcatgttga
     3901 atattctgct gtctttatgt ataagtttat caaatgtaag ctgaatgtaa tatccgtatc
     3961 ttctgttaac aggcaataac tcagaaaaga atttaagctg gtattttgag aacatagcct
     4021 gaataacaag tgttaaaaat actgtaaaaa gtaacgatac tactaaaacc catgaaagat
     4081 tcagcataag tacaataagt cctaaaactg taacaacctg attaatgaat gtgactgctg
     4141 tatttataag ccttgacaat gaagactggt ttctcattgc aaatgtggct ctttctttta
     4201 aatcaagata atatggatct tcaagatatc tgtattcaat ggacattatt ttttctgaaa
     4261 atgctctttc tatctgccag aaaagatctt tctcctgcac atccaaaagt ctctttagcg
     4321 tcttttttgt taaagtgaag aaagcattac caagcactat taccaatatc catgatataa
     4381 gcattgtcct gtcctggtta cccattaatt cctcgatcag atattttgga aatatgacat
     4441 tggttaagat gtgacctgaa aaggcaagcg aattcaagaa caaggttata aaatatacag
     4501 gttttaattt ccacacaagg gatactacca atgatatctt ttttgaagct ttcatatctc
     4561 ttcctcctgt gtctgataat attttccctg gacaatgaac atatcatgat aagctccgcc
     4621 aagcgccata agctcatcat gagtaccata ttcagataag ccgtcattgt ctataagggc
     4681 tattgaatca cagaatttgg ttgatgccag cctgtgggag atatatatag ctgttcttcc
     4741 atgaataagc gtatcaaact gtgaataaat tgatgcttca gccaaagcat caagcgaagc
     4801 agttggttca tccattatca cgcagttacc gcccttgtat aaagctcttg ctattgctaa
     4861 tctttggttc tctcctcctg aaaagactac tccgtcttct tcaatcactt ttaacatcat
     4921 actgtcgacg cctttatcca gtgatttaac cttatcatac aaatcaacct gctttaaaac
     4981 ttcatccact ctttcataat ctatattatc tatcgagcag gcaatattct gagctattgt
     5041 taatgctaat atattgacct cctgaaatac tacacagaac atagagaata attcctgcct
     5101 gtcatattca tttatatctt tcccattaat taatattttg cctgagtcag ctttaaacat
     5161 tccagtaagc agttttacaa aagtggtctt gccagctccg tttataccta ccagtgcaag
     5221 cttactagct ggaggtatgt gtaaagaaaa attcgtaaag ataggtttat cagtacctgg
     5281 ataagtaaaa tttacatcaa taaattcaat ttccaaaggc atatcctttg aaacagcagg
     5341 taatgttccg cctttattac ctaaatcttc atcaaagaat ttatacagat cttttacata
     5401 catgtattcc tgcataatat atgttacatt ttctgataaa gaggtcatct gaactgtaag
     5461 tgtcacacat gccgctatat acattgaata gtcagcaatt gacatacctt ttactacaag
     5521 tattgttagc attccatatg cagctatatc tgcaattatt agagtcagta aagataaaaa
     5581 ccccaataaa tactctttat tctttataac cttgaaaata tcaatgtacc ctgatatctc
     5641 ttttttaaag ttatcaagta ttcttgtttt aagatcatac atccttatgt ctttaccaaa
     5701 agaaaagtcc ttagatgtag acgaataata gcttactctt ctttctttct tcgataactc
     5761 ttcttttctt ttatactgat atttttgcac aattatcgca atatatgttg tcacaaatat
     5821 atgaagtatc aaggctaata gtatccatat gctttttatc cctataaaca ctgataatgc
     5881 taatactatt aatactaact gaggcaattc aaaaagttta tgaaaaattc cctcaactcc
     5941 gttattattt gatgatgttg attcaaatgc gcgttctctg ctattaaaga attcagcctg
     6001 ctccatatac ttgtaatcca ttgtaagaag cttaacagcc tgttctttga tatagtctat
     6061 gcgtaaggtt gtaatacgag gatatgtata atccaaaagc cattctttta aaaagtatac
     6121 agtagcaccg gatatgaaga atataagagc cagtcttact atacctgata ccaaagcctc
     6181 cccttcggta agataaccta taatcacctt tggcagtgca acagatatta caggaaccat
     6241 tattgcagct aacgtatata tagcacacag aaaatatatg cttttgtcct ctacccatgc
     6301 atacccaagc attcttttta atgctgaatt attctttttg ctcactcaaa acctctttcc
     6361 atttagcaac tagattatct ctgtcttctt tgctatcatt catattaagc agcttttcaa
     6421 cagcttcaac tgcagacttc cctatatcat cacaaaagaa atgctcctta ccagaataaa
     6481 accttatact gttacaggaa aaatcaggat tggaatcaaa cagccttgct tttttagcaa
     6541 tatctttcat aagcagaaca acagaatcct tatccttcat agcataacgc agcattacct
     6601 gaatagtatc aagcatcatc cccattttaa taagatatga gggtctatcc ttatattcca
     6661 gtgcatttaa agtattctta agccatataa tcatctcaag cgcttcgcta tgtctaccca
     6721 tttcaacaaa gcaggtacac attcctgtta tacagatata tatatttaca agattatcaa
     6781 tataactttc cgaaaaagca atcaaagcct gatcatattt tttcatctca gtatagcttt
     6841 ttcctatcca ggagttattt accccattta cattgtattc tttgaatata tctatagctt
     6901 catcatatct tttttctgaa gataaaataa ttgcaatcct gctttttata gaccctatac
     6961 ttatatccgg attgctattc tgctctataa ggctgcatga atgctctaat aatactaatg
     7021 attgagccac tgcctctttg tcttctcttt cccatcctaa atttgatagt gttattcctg
     7081 attgataaac aatatcgaaa tcattaggat acttaagcaa agccttaaat atttcatctt
     7141 taacagctga atagtctttt ttattactta atgatttaat taaatttaaa gtgtcagaaa
     7201 gatttcgaga tctgtatgaa tagcccaata atgaatctac tgataactca aaaaaatctg
     7261 cgatccttac tatcatgcct atttcaggat tcatctgacc gctttcccat ttagaaacag
     7321 cacctactga cactcccatt gcttcagaaa gctgttcctg tgtcattcct cttttctttc
     7381 tatatttctt gatattttcg tttaaatgca gttccataac cccgtctttg acagttagca
     7441 ccataatctt taggagcaga taccgtatat aa
//
LOCUS       DAOSXM010000097         7465 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2111
            NODE_6411_length_7465_cov_11.695277, whole genome shotgun sequence.
ACCESSION   DAOSXM010000097 DAOSXM010000000
VERSION     DAOSXM010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864178
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 7465)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7465)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:24:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,261
            CDSs (total)                      :: 2,229
            Genes (coding)                    :: 2,218
            CDSs (with protein)               :: 2,218
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 3 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7465
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6411_length_7465_cov_11.695277"
                     /isolate="AMR_MDS_2111"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(208..594)
                     /gene="rpsI"
                     /locus_tag="PLQ68_09685"
     CDS             complement(208..594)
                     /gene="rpsI"
                     /locus_tag="PLQ68_09685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HXK72965.1"
                     /translation="MTQYCGTGRRKSSVARVRLVAGTGKITINEKDISEYFGLETLNI
                     IVNQPLDLTKLKGKYDVLVNVKGGGFTGQAGAIRHGIARALLEANPDLRSALKKAGYL
                     TRDPREKERKKPGLKKARKASQFSKR"
     gene            complement(609..1043)
                     /gene="rplM"
                     /locus_tag="PLQ68_09690"
     CDS             complement(609..1043)
                     /gene="rplM"
                     /locus_tag="PLQ68_09690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004620795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HXK72966.1"
                     /translation="MKTFMTNAESIDKKWYVVDASGQTLGRFASEIAKILIGKNKTTF
                     TPGVDAGDYVIVINAEKIEVTGKKESQKMYRRHTGYIGGLKEVVYKDMMAKHPDRIIT
                     AAVKGMLPKNKLGRHMLSKMKVYAGSEHNHQAQNPEVLELNK"
     gene            complement(1199..1777)
                     /gene="yfbR"
                     /locus_tag="PLQ68_09695"
     CDS             complement(1199..1777)
                     /gene="yfbR"
                     /locus_tag="PLQ68_09695"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: protein motif:HMM:NF003009.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002953 - 5'-deoxynucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HXK72967.1"
                     /translation="MKPGSFFAFIHRIGYINRWGLMRNTSYENLKEHSYDVAVIAQGL
                     ALIGNAKFNKNYDVNRITSAAMFHDVNEALTGDLPTPVKYANKDIETAYKKIEADAVN
                     NILMMQDEIIKDKYVEYFNPSAEDKLIIKAADKISGFIKCKEEKDRGNKDFDKAYNSL
                     KKSIENSDLPEVKYFIKNMLPHYNLILDEFLE"
     gene            complement(1774..2361)
                     /gene="loaP"
                     /locus_tag="PLQ68_09700"
     CDS             complement(1774..2361)
                     /gene="loaP"
                     /locus_tag="PLQ68_09700"
                     /inference="COORDINATES: protein motif:HMM:NF033641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator LoaP"
                     /protein_id="HXK72968.1"
                     /translation="MTQSYDTPINEKQGYINSWYVFYVKSGEENKAKDNLVSICERKD
                     IKLDFVIPSAIKFIKKGKQRIKTYKTIFPGYVFANGNIDTEIYTILKGIPGVYRLLRD
                     RNTFTILPVPAEEMELLASIMNVHGVIEAPEVSFEEGQTAVITKGPLTSFKGKIVSVD
                     KHKNKLTLLIPFLGEERKVEVGFLFAEAINKEIVV"
     gene            complement(2391..>2681)
                     /locus_tag="PLQ68_09705"
     CDS             complement(2391..>2681)
                     /locus_tag="PLQ68_09705"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF013632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HXK72969.1"
                     /translation="LKDKLIGAMAGGGFLSFMNSLSIIIFKKEGIGGGDIKLCFACGL
                     VLGIDLMIVAFILSFIPAAIYTMFVCRKSSNDIALGPFLCVSIICSYCLGMS"
     assembly_gap    2682..2781
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2978..5296
                     /locus_tag="PLQ68_09710"
     CDS             2978..5296
                     /locus_tag="PLQ68_09710"
                     /inference="COORDINATES: protein motif:HMM:NF036578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HXK72970.1"
                     /translation="MFDIHIKKEIEWKAFWIHDPIFKKQPIVQPIDAKRSKYLRTEIH
                     NRHTLFRKEFEVKDIKIKSAKLYVTADDCYKAYINGQFIAYGPAQAFCFSYNYNVFEI
                     KKLLKPGINTLAFHVFYQGMLNMALISADNLNGMICMLEIEYADGYTQMISSDSSFRC
                     HQLMSYTFDKVYGYETQFAENIDLNYFPYGWNKKGFDDSDWITPFISSVPYPMFYNLN
                     QQITPTVSFEKFYPEKIVRKGKGHYLLDFGQEICGNTTVTASGKKGQVITIWHGEELD
                     EDNKSARHNMRCGCDYEETITLSGNVKSDKAEFFDFKAFRYIEVFNFPGNFTKKSIYV
                     NHRHYPIPKNHAVFETDNTLFEKIWKMCEKGIIEGTQESYIDCPTREKGAFLGDGLIT
                     GLSHLCYTNDERIMKKFLYDCISSARLCPGLFSTTVTYDASQMIDYSLVFPIVLNKYF
                     EYTNDIKMIKDSLNVLEGIISYYAKFYEPEGILGHIKHVHRDQYTVLVDWPVVYRDGY
                     DDDTIKDANCFGNLAYLGILKETIKAYSYTDNELRVKELTDKATQLEQAIINAFYDYD
                     TGLFNISRTSSCHNFHDAVFALYFELNLPKGHKPLLDYIKQRGIVCGVYFTYYYFSVL
                     YRHMEYDKIFDLMISNERNSWKTMLSTGATTCIEAWHPDDKLNCSLCHPWSSTPMIFN
                     DIFYGISSYGIGYEKLQFKPRIPKQLNSGKYTKPTPLSDITVSFERKGNKVIYHLTLK
                     KACRIFVYLSDKEKGHFIDAPVGITEFVQVLD"
     gene            complement(5293..6999)
                     /gene="argS"
                     /locus_tag="PLQ68_09715"
     CDS             complement(5293..6999)
                     /gene="argS"
                     /locus_tag="PLQ68_09715"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012062928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HXK72971.1"
                     /translation="MKEIKSIIAKKLSENIEQLSWREIMGNLEYPPEPDMGDLALPCF
                     KLSKVLRKSPVAIADSLVDLLNIDLVERCESKSGYLNIFLKREYMVNNILKNIVDLKE
                     EFGKSDENAGKTVCIDYSAPNIAKPFHIGHLRSTVIGHAIRRLHEFRGYKCIGINHLG
                     DWGTQFGKLIVAYKNWSSKELIEEKGTEELVRIYVKFHAEAENNPSLNDEARKWFSKL
                     EKKDEEAVELWKWFKDISLLDIEKTYKMLDISFDSYAGESFYNDKMEPVIEELKQKNL
                     LQSSDGAMIVNLEEYNMPPCLILKSDGSSLYATRDIAAAMYRKKIYDFAKCIYVTATA
                     QDLHFKQLFKVVELMGYEWAKDLIHVGFGMVSMGGAKLATREGNIIYLDDLFKEASKR
                     VLDIINEKNPDMEDKEQKAIDIGVGAIVFSDLSNNRIKDIDFSWEDALNFDGSTGPYV
                     QYTYARACSVLRKSEIADDEINMNGIEPGHMTNDEEFSLLKTLSLFNEKIDQALKDLE
                     PSAIARYLIDVCMVFNKFYNSHTILNAQENKKFRILLTSAVKYVLGNGLQIIGLKKTE
                     RV"
     gene            complement(7120..7398)
                     /locus_tag="PLQ68_09720"
     CDS             complement(7120..7398)
                     /locus_tag="PLQ68_09720"
                     /inference="COORDINATES: protein motif:HMM:NF016839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HXK72972.1"
                     /translation="MKSNIRKRLITLISIAFIIYFIYVIFSQQPRIDMLMAEKQEVQE
                     KIEEQDLIYNQLQYYFEIAGTDEYIELIARSRLGYVKPNDIVFIDLNK"
BASE COUNT         2289 a         1397 c         1087 g         2592 t
ORIGIN      
        1 tagtctttca tttttttcga cttgcttaat actggaattt aagttttcac tttaccatat
       61 ttactgttac atgtgtaagt ttttcgcatt atagtgctgg atattttttc gcaaaaaaaa
      121 ctccccagaa taagttttcc agggagtttg aaatcgtatt gaatttctct ggagagttta
      181 cctctggaga gtttacctct gaagagctta tctctttgag aactgtgaag cttttcttgc
      241 tttcttgagt cctggtttct ttctttcttt ttctcttggg tctcttgtaa ggtatccggc
      301 tttcttaaga gcagatctta aatctggatt tgcttcaagt aaagctcttg ctataccatg
      361 tctgatagca ccagcctggc ctgtgaatcc gccaccttta acattaacca atacatcata
      421 tttccctttg agctttgtaa gatcaagtgg ttgatttaca attatattaa gtgtttcaag
      481 tccgaaatac tcgctaatat ctttttcatt tatagttatt ttgcctgttc ctgcaactaa
      541 tctcactctg gcaacagaac tttttcttct acctgttcca caatactgtg tcataactta
      601 acctcccgtt acttattcag ttccagaact tctgggttct gtgcctgatg attatgttcg
      661 cttcctgcat ataccttcat cttgctaagc atatgtctgc caagtttatt ctttggaagc
      721 atacccttaa cagctgctgt tataattctg tctggatgct ttgccatcat atccttgtat
      781 actacttcct ttaatccgcc gatgtaacca gtatgtcttc tgtacatttt ttgtgattct
      841 tttttgccag taacttcgat tttttcagcg ttaattacta ttacataatc accagcgtca
      901 acaccaggtg taaatgtagt tttgtttttt ccgattagaa ttttggctat ttcgcttgca
      961 aatctgccaa gtgtctggcc gctagcatct acaacatacc acttcttatc tatactttca
     1021 gcatttgtca taaaagtctt catattgtgc ttgttctcct ccataattaa tgccgcttat
     1081 taacggcatg ggttatttta atacatatat catatattgt caacaacaaa ttttatatat
     1141 ctctctaagc ctttttaata ctcccgattg ctctttaaat ctccgttttg ctcacacttc
     1201 attccaaaaa ttcatcaagt ataagattgt agtgaggcag catgttttta ataaaatact
     1261 taacttcagg aagatccgaa ttttcaatgc tttttttcaa agaattgtat gccttgtcga
     1321 aatctttatt acctctgtct ttttcctctt tgcattttat aaagccggat atcttgtcag
     1381 cagcttttat aatcagttta tcttctgctg atggattgaa gtattctaca tatttatctt
     1441 ttataatttc gtcctgcatc ataagtatat tatttactgc atctgcttca attttcttgt
     1501 aagctgtctc tatatcctta tttgcatatt taacaggtgt tggaagatcc ccagttagtg
     1561 cttcattaac atcatgaaac attgctgctg atgttattct gttaacatca taatttttat
     1621 tgaatttagc atttcctatt aatgcaagac cctgagctat cactgcaaca tcgtaactgt
     1681 gttccttaag gttctcataa ctggtgttac gcataagccc ccatctgttt atatatccta
     1741 ttctgtgtat aaatgcgaaa aaactacctg gtttcatact actatctcct tgtttattgc
     1801 ttctgcaaac agaaacccta cttcaacttt cctctcttct cctaaaaatg gaataagaag
     1861 agtaagtttg tttttgtgct tatcaactga tacaatttta cccttaaagc tggtaagcgg
     1921 accttttgtt ataactgctg tctgaccttc ttcaaaactt acttctggag cttcaataac
     1981 cccatgtaca ttcattatgc ttgctaaaag ctccatttct tctgcaggta caggaagtat
     2041 cgtaaaagta ttcctgtctc ttaagagcct gtacacgcct ggtattccct ttagaatggt
     2101 ataaatttct gtatcgatat taccgtttgc aaatacatat cctgggaata tagtcttgta
     2161 tgtctttatt ctctgcttgc ctttttttat gaattttatg gctgaaggaa ttacaaaatc
     2221 cagtttaata tcttttcttt cgcatatact aacaaggtta tccttcgcct tattctcctc
     2281 accgctttta acgtaaaaca cataccatga atttatatac ccctgttttt cgtttatagg
     2341 agtgtcataa ctttgcgtca taaaaatacc tctttatact attttatcat ttatgacatt
     2401 ccaagacaat atgaacaaat tatggaaaca cacaaaaaag ggcctaatgc gatatcgtta
     2461 ctgcttttcc tgcatacaaa cattgtatat attgctgctg gaataaagga gagaataaat
     2521 gcaacaatca ttaaatctat acccagcaca aggccacatg caaaacacaa tttaatatca
     2581 cctcctccta ttccttcttt tttaaatatg attattgata aactattcat aaaagacaaa
     2641 aaaccgccac ctgccattgc tcctattaat ttatctttca annnnnnnnn nnnnnnnnnn
     2701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2761 nnnnnnnnnn nnnnnnnnnn ntcatcaaca tatgataagc ttcttaaggg ttgtataaga
     2821 agttttgaat atgctttgga tattaatgta aaaagaatat attccatata tcacctgaaa
     2881 agattttaca aaacaatttt gtatttaaac actgacaatt taaaatacat taaataatgc
     2941 agataattgt tatacaataa agaggaggtg aagacgcatg tttgatatac acattaagaa
     3001 ggaaattgag tggaaagctt tctggataca tgatccaatt tttaaaaaac aacctatagt
     3061 gcagcccatt gatgcaaaaa ggtctaaata tcttcgaaca gaaatacata acaggcatac
     3121 tctttttcgc aaggaatttg aagttaagga tattaagatt aaaagcgcaa agctttatgt
     3181 aacagctgat gactgttaca aagcatacat taatggtcaa ttcatagcat atggtccggc
     3241 acaggcattc tgtttttcat ataattacaa tgtatttgaa ataaagaaac ttctaaagcc
     3301 tggaattaat accctggctt ttcatgtgtt ctatcagggt atgcttaata tggccttaat
     3361 ctctgcagac aatctaaatg ggatgatatg tatgcttgaa atagagtatg cagatggcta
     3421 tacccagatg atatcttctg actcctcttt tagatgccat cagcttatgt cttatacatt
     3481 tgacaaagtg tacggatacg aaacacagtt tgcagaaaat atagacctta attattttcc
     3541 ctatggatgg aataaaaaag ggtttgacga ctcagactgg ataaccccgt tcatatcctc
     3601 agttccatat ccaatgttct acaacttaaa ccagcaaatt acacctacag tttcctttga
     3661 aaagttctat ccagaaaaaa tagtcaggaa aggaaaaggc cattaccttt tggatttcgg
     3721 gcaggagata tgcggaaata ccacagttac tgcttctggt aaaaaaggac aggtcataac
     3781 aatatggcat ggcgaagaac tagatgaaga taataagtct gcaagacata acatgcgatg
     3841 cggctgcgat tatgaagaaa caataacact aagcggtaat gttaaatcag ataaagcaga
     3901 attctttgat ttcaaagcct tcagatatat tgaagttttt aattttccag gaaatttcac
     3961 caaaaaaagt atatatgtaa atcacaggca ctatcctata ccaaaaaatc atgcagtctt
     4021 tgaaacagat aacaccttat ttgaaaaaat atggaaaatg tgcgaaaagg gaataattga
     4081 aggaacacag gagtcatata ttgactgtcc tacaagggaa aagggagcat tcttaggaga
     4141 cggacttatt actggtcttt ctcacctttg ctatactaat gatgaaagga taatgaaaaa
     4201 atttctttac gactgtataa gttctgcaag attgtgtcca ggtttgtttt caacaacagt
     4261 cacatatgat gcatcacaga tgatagatta cagccttgtc ttccctatag ttttgaataa
     4321 atactttgaa tacacaaatg atatcaaaat gattaaagat tcattaaatg ttttagaagg
     4381 aatcatatct tattacgcta agttttatga acctgaaggt atactgggac atattaagca
     4441 tgtacacaga gaccagtata cagtacttgt agactggcct gtagtatacc gagacggata
     4501 tgatgatgac actattaaag atgcaaactg ttttggaaat cttgcgtatc ttggaatact
     4561 taaggaaacc ataaaagcat atagttatac agataatgaa ttaagagtca aagagcttac
     4621 agacaaagcc acacagcttg aacaagccat aattaatgct ttttatgatt atgatactgg
     4681 tcttttcaat atatccagaa cttcatcttg ccataatttc catgatgctg tgtttgcttt
     4741 gtattttgaa ttaaacttac ccaaagggca caaacccctg ttggattata tcaagcaacg
     4801 aggaattgta tgcggagtgt actttacata ttactatttt tctgttttat acaggcatat
     4861 ggaatatgat aagatttttg accttatgat ttcaaatgaa agaaattcat ggaagacaat
     4921 gctaagcact ggtgctacca catgtataga ggcatggcac ccagatgata aattgaattg
     4981 cagtctttgc catccatggt ccagcactcc gatgattttc aatgatatct tttacggaat
     5041 atcatcatat ggtataggtt atgaaaaatt gcagttcaag ccaagaatac caaaacagct
     5101 taacagcggt aagtatacaa aacctactcc cctttcagat ataacagtaa gttttgaaag
     5161 aaagggaaac aaagttatat atcatcttac tctgaagaaa gcgtgcagaa tttttgtata
     5221 tttatcagat aaagaaaaag ggcatttcat agatgcccct gtaggtataa ctgaatttgt
     5281 tcaggtattg gactagaccc tttcggtttt tttaagtcct attatttgta atccatttcc
     5341 taatacatat ttgacggcag atgtcagcag aatcctgaac tttttattct cttgcgcatt
     5401 caatatcgta tgggagttgt agaacttatt gaagaccata catacatcaa ttaagtaacg
     5461 tgctattgct gatggttcta aatcttttaa agcctgatca attttctcat taaatagtga
     5521 taatgtcttt aataaagaaa attcttcgtc atttgtcata tggcctggct ctattccatt
     5581 catattaatt tcatcatcag caatttctga ttttcttaaa actgaacatg ctcttgcata
     5641 agtatattga acataaggac ctgtagaacc gtcgaaattc aaagcatctt cccatgaaaa
     5701 atcaatatcc tttattctgt tatttgaaag atcagaaaat acaatagctc ctacccctat
     5761 atctatcgct ttctgctctt tatcttccat atcaggattc ttctcattga ttatatccaa
     5821 aactcttttg gaagcttctt taaataaatc atcaagatat ataatgtttc cctcacgagt
     5881 agctaatttg gctccaccca tggaaaccat tccaaatcct acatgaatca aatcctttgc
     5941 ccactcataa cccataagtt caactacttt gaataattgt ttaaaatgaa gatcctgtgc
     6001 tgttgctgtg acatatatgc attttgcgaa atcatatatt ttctttctat acattgcagc
     6061 agcaatatcc cttgtagcat ataaagaaga accgtcagat ttaagaataa gacatggggg
     6121 catattatac tcttctaaat ttacaatcat agcaccatca ctgctttgca gaagattctt
     6181 ctgtttcagt tcttctataa caggctccat tttatcatta tagaaagatt cacctgcata
     6241 actgtcaaat gatatatcca gcattttata tgtcttttcg atatctaata aacttatatc
     6301 cttaaaccat ttccaaagtt cgactgcttc ttcatctttc ttttccagct ttgaaaacca
     6361 ctttctcgcc tcatcattca atgaagggtt attttccgct tcagcatgga attttacata
     6421 tattcttacc agctcttctg tgcctttttc ttcaattaat tccttagaac tccagttttt
     6481 atatgctaca ataagttttc caaactgagt cccccagtca ccaagatgat taattccaat
     6541 acatttatat cctctgaact catgaagcct tcttatggca tggcctataa ctgtagaacg
     6601 caaatgccct atatggaaag gttttgcaat attaggtgca gaataatcta tacacactgt
     6661 ttttcctgca ttttcatcac tttttccgaa ctcctctttt aaatcgacta tatttttcag
     6721 aatattattt accatatatt ctctttttaa gaatatgttc agatatccgc tcttactttc
     6781 acacctttca accagatcga tatttaacaa atccacaagt gaatcagcaa tagccacagg
     6841 agactttctt aaaactttac taagcttaaa acaaggtaat gcaagatcac ccatatcagg
     6901 ctctggagga tattcaagat ttcccataat ctccctccag ctaagctgtt caatattctc
     6961 actaagtttt tttgctatta tactttttat ttctttcata aaaatctctt ttcactattt
     7021 attattcaca ggacaattaa tacaaataac ccgtgtgatg ttactgaatt atcccttata
     7081 ttaacatatt tatattcttt ttccaataag gctggattat catttgttca aatcaataaa
     7141 aacaatatca ttaggtttga cataacctaa ccgtgaccgt gctatcaatt caatatattc
     7201 atcagttcca gcaatttcaa agtaatactg cagctggttg tatattaaat cctgttcttc
     7261 aattttttcc tgaacttcct gtttttccgc cattaacata tctattctgg gctgttgaga
     7321 aaaaatgaca tatataaaat atataataaa tgcaatggat atcaatgtaa ttaatctttt
     7381 tctgatattg cttttcatta aaagccttcc tctttagtgt aattgtattt tatcataccg
     7441 atatattttg tcaatgtatt gaaaa
//
LOCUS       DAOSXM010000098         7320 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2111
            NODE_6593_length_7320_cov_8.570268, whole genome shotgun sequence.
ACCESSION   DAOSXM010000098 DAOSXM010000000
VERSION     DAOSXM010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864178
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 7320)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7320)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:24:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,261
            CDSs (total)                      :: 2,229
            Genes (coding)                    :: 2,218
            CDSs (with protein)               :: 2,218
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 3 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7320
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6593_length_7320_cov_8.570268"
                     /isolate="AMR_MDS_2111"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..575)
                     /locus_tag="PLQ68_09725"
     CDS             complement(<1..575)
                     /locus_tag="PLQ68_09725"
                     /inference="COORDINATES: protein motif:HMM:NF024734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HXK72973.1"
                     /translation="MNRDMIVKLLKENNGIIKSEDIATLGVDNKVLQRMALKGELERI
                     SHGLYLTANYMKDEYMLVQYRCSKCVFSHETALYFHDLSDRTPFQLMMTIPSGFNSRL
                     LKDIEKYKFFYSNKNLHNIGVTSSVTSYGNQIKLYDKERTICDCIKKIDKLDSNIVLT
                     AVKRYMSEPGADYTKLLRYAEIFKVRDTVKQ"
     gene            965..1879
                     /locus_tag="PLQ68_09730"
     CDS             965..1879
                     /locus_tag="PLQ68_09730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009614769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1848 domain-containing protein"
                     /protein_id="HXK72974.1"
                     /translation="MIISASRRTDIPCYYSEWFMNRIQSGYLLTRNPMNNSQISRISL
                     SPKIVDCIVFWTKDAKNIIPHLKELDLLGYKYYFQFTLNPYDKCIEPNLRDKDDIIDT
                     FIELSKLIGKERIIWRYDPIILNDICDIDYHKTQFEMMCKKLSSYTEKIIISFVDMYA
                     KVKTPLIRPLREDEIVELSVYIRRTAKTYGLSVYACCESMDLSPYGILKSSCIDKDTI
                     ERVCDIKLDLKQDKNQRNGCGCYQSIDIGAYDTCANGCIYCYANKNHKNSEKRYLTHN
                     PKNELLIGTVLENEVIKDREQTSDKMKY"
     gene            1974..2996
                     /locus_tag="PLQ68_09735"
     CDS             1974..2996
                     /locus_tag="PLQ68_09735"
                     /inference="COORDINATES: protein motif:HMM:NF014114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HXK72975.1"
                     /translation="MKFPKLLKKGDTIGVCAPSSGASGEILSVRLNNAISNLKYLGYN
                     VIVTDSIHNNDVKCVSADSMTRATEFMSLYENSDVSVILPPWGGEFLMDILPLLDFEK
                     IASLPSKWVCGYSDVTTLTFPLTLICDIATVHGSNLMNMGFARIHETDLRAFHVMSNL
                     KTKQKSWDTWGGFSSWNDLTSDVYKLDNPSTWKSLQKDTHVTFKGRMIGGCMDTLCKL
                     LGTRFAPIEAFINKYKDDGFIWTLESCEMTAPEIYRTFWQIRESGWFKYCNGIIFGRP
                     RGCDNKRDFTLIDALEQGLGNLNVPVIYDADIGHVPPQIQIVNGSIGRVEFADGKATI
                     IQSMEI"
     gene            3009..3431
                     /locus_tag="PLQ68_09740"
     CDS             3009..3431
                     /locus_tag="PLQ68_09740"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXK72976.1"
                     /translation="MFYDIKERIYDSVVREIMAQSSFDQSREAMDRKAEEFNRHVSWQ
                     LYGWIEDEEIVGICGFVVYRDYIEILNIAVAQYAQRKGIGSRMITELWNKYKITIKAE
                     TDDDAVDFYRKCGFSTIEISKYNVRRWTCVLEDKQVIS"
     gene            complement(3442..3891)
                     /locus_tag="PLQ68_09745"
     CDS             complement(3442..3891)
                     /locus_tag="PLQ68_09745"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXK72977.1"
                     /translation="MNITYVNEISAQEVNHLRSSIGFRKINLEQLNASLAGSAYIVAA
                     YSKEEVIGMSRIIWDGGSVALIHDIIVVPQYQMQGIETEMLNRIFDFLKSKLKPGFGI
                     QVDIRAWNNQVEYFESVGFQISTTQKRGIPMHICLTNQIELTDNMSE"
     gene            complement(3888..4766)
                     /locus_tag="PLQ68_09750"
     CDS             complement(3888..4766)
                     /locus_tag="PLQ68_09750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009537577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HXK72978.1"
                     /translation="MNSIISQMLNQYNTQTQHDRINSIKQVIQEVTLCGLSRAGFFKN
                     AAFYGGTALRIFYGLDRFSEDLDFSLKTSDSSFNFNEYLPLLEKEIRSYGLNFDVSIK
                     QKSADTNITSAFLKGNTKEHILMFYSDKLIARSINSNELIKIKVEVDTDPPPYACFET
                     KYQLLPIPYEISLYDVSSLFAGKIHAILCRSWKNRIKGRDLYDYIFYLSRKTPVNLKH
                     LTARLIQTGNISENSQININDIKKMLYERFNNIDYSQAKQDVLPFIKNAASLDIWKAD
                     FFCIITEQLSDKEECQ"
     gene            complement(4759..5352)
                     /locus_tag="PLQ68_09755"
     CDS             complement(4759..5352)
                     /locus_tag="PLQ68_09755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009537578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72979.1"
                     /translation="MIKTTAMLLEELKEYVNPTAKIRRMVTNSELIPVVRGIYVTDKN
                     IPGYYLASIIYGPSYLSFEFALAYHSLIPETVYTYTSATFKKKKAKQYENPFGTYTYR
                     DVPSKAYPLGVNLHHENGYSYQIASAEKAVCDQLYILSPVNNQKELENLLFDDLRIDS
                     DEFFRMNLSDLLEISSNYRTQNHKLLQAYIRRRMKNE"
     gene            5570..6217
                     /locus_tag="PLQ68_09760"
     CDS             5570..6217
                     /locus_tag="PLQ68_09760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72980.1"
                     /translation="MTYIEYLCKEIRKIQPGEPIYSKHLSKMVENAFNIDYKAAAAAV
                     AVAMKRIIGNKVVPDLRSYQKGIFYRTLETVFGETGINKNKLIADKYLADNIGYETGP
                     IILHRLGLTSQMPKQLLLATNAAKDCMRVDNRLGVVIKVPKVKVTAENKAYLQILDVL
                     DMMDDNPIDEKEPHYIVAEHIKKQNLQYDILLALADKYYNQKTILQLARTASAVA"
     gene            6222..7199
                     /locus_tag="PLQ68_09765"
     CDS             6222..7199
                     /locus_tag="PLQ68_09765"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HXK72981.1"
                     /translation="MKLHNDRDSFIVLINDIQAKTGYRADVIEKDYYVVLMLKELAYK
                     QNSGLQAYFKGGTALYKALNTTNRFSEDIDVSVDTRGCCRSQNDKRLENASKKYISLP
                     RDSAQGRTNRSEVISIYTYIPITVYDQGDMLQRFGKVKVEATSFTISEPVEDLEISAI
                     IYNQATEEQKNILRDIYDVKPFSIKTISLERIFIDKLFAAETYTRKSSNRRSAFDASK
                     HIYDLAVLSDKLRIIELLSNTEKMSRLLEIRLQEEYMRLDGIPDILPNQFMFFRDAGT
                     NSDIRKAYEIMQNQYVFRLTDRIEFDNALKTIDRMRISLSKNKAWLLSK"
BASE COUNT         2458 a         1165 c         1278 g         2419 t
ORIGIN      
        1 tattgtttta cagtatctct gactttaaag atttctgcat atcttaatag tttggtataa
       61 tcagcaccag gttctgacat atatctttta acagctgtta atacaatatt tgagtcaagc
      121 ttatcgattt ttttaataca gtcacaaatg gttctctctt tgtcatatag tttaatctga
      181 ttgccatatg atgtgactga agaagtgaca ccgatattat gtagattttt attactatag
      241 aaaaacttat atttttcaat atctttcaga agcctgctat taaatcctga aggaatagtc
      301 atcattaact gaaaaggcgt tctgtcactt aaatcatgga agtatagagc tgtttcgtgt
      361 gaaaatacac attttgagca tctatactga actaacatat attcgtcttt catataattt
      421 gcagtaagat atagtccgtg actgatacgt tcaagctctc ctttcaatgc cattctctgc
      481 aacactttgt tatctacacc tagagttgct atatcttctg actttataat cccattattt
      541 tcctttaata attttacaat catatcccta ttcatcctat gaactctcct aaggcctaat
      601 tgttagtaaa aatcacatat tatactcata aataagacaa gacttattct gaaaaatgag
      661 atataacata ttaatcaatt ttatcctata ttgtataagt tagtaactcc caatattctg
      721 taacatcagt ttcgaaatat gaaaataatt aaattttcga aaccttaagt catattttca
      781 tagaacatag aaatacactg cttactaatt attctttctt tattatccat attatctatt
      841 ttatcaattt ctaaacttga catactattg tcatgttttt atgtaataat ataaatgatg
      901 tttctgtcga attaattgaa agtgtaaaaa ctggcggaaa gcaatgtgtc atgaggataa
      961 gaagatgata atatcagcat caagacgaac tgacatacca tgttattata gcgaatggtt
     1021 catgaaccgg atacaatctg gttatttatt aacccgaaac ccaatgaata attcacagat
     1081 ctcacgtata tcactgtcac ctaagattgt tgactgtata gtattttgga caaaagatgc
     1141 taaaaatatc attccacatc taaaagagct tgacctgctt ggatataaat actattttca
     1201 atttactctt aatccgtatg ataaatgcat tgaaccgaat ttgagggata aggatgatat
     1261 tattgataca ttcattgaac tatctaaact tattgggaaa gaacgcataa tctggcgtta
     1321 tgacccgatt atccttaatg atatttgtga tattgattat cataaaacac aatttgagat
     1381 gatgtgtaag aaactgtctt cctatacaga gaaaattatt atcagttttg ttgatatgta
     1441 tgcaaaagta aaaacacctt taatccgtcc gctcagagaa gatgaaatag ttgaattatc
     1501 tgtttatatc agaagaacag cgaaaacata tggtttatct gtttatgcat gttgcgaaag
     1561 catggacctt tctccatatg gtatcttaaa gtccagctgc attgataaag atactattga
     1621 aagagtatgt gatattaagc ttgatttaaa gcaggataag aatcagagaa acggatgcgg
     1681 atgttatcag agcattgata ttggagctta tgatacatgt gcaaatggct gtatatactg
     1741 ctatgcaaac aagaatcata agaattctga gaaaagatat ctaactcaca atcctaagaa
     1801 tgagctgtta attggtacag tattggaaaa tgaagtaatt aaagacagag agcaaacatc
     1861 agataaaatg aaatattaag gtattcttgc ttatataatc atttctagaa aattatatgt
     1921 ttgattttac agttatctga tgtatataat acttgtaacg aaaagaggtt atgatgaaat
     1981 tcccaaaact tttaaaaaaa ggagatacca ttggcgtatg cgctccttca tccggtgcct
     2041 ctggagaaat cttatcagtt cgattgaaca atgcaatatc aaatctgaaa tacttaggct
     2101 acaacgttat tgttactgat tcaattcata ataatgatgt gaaatgtgtt agcgctgata
     2161 gcatgacgcg tgcaacagaa tttatgagtt tgtacgagaa ttcagatgtt tcagttatcc
     2221 tgcctccctg gggaggagaa ttcttaatgg atatcctgcc tcttcttgat tttgaaaaaa
     2281 tagcttcact tccttcaaaa tgggtatgcg gttattccga tgtcaccaca ttaacctttc
     2341 ctcttacatt aatttgcgac atagcaactg tacatggttc taatctgatg aatatggggt
     2401 ttgctcgtat tcatgaaacg gacttacgag cttttcatgt tatgtctaat ctaaagacca
     2461 agcagaaaag ctgggacaca tggggcggat tttcaagctg gaatgattta acctcagatg
     2521 tttacaagct tgataatcct tctacatgga agtcattaca aaaagatacg catgttacat
     2581 tcaaaggccg tatgattggc ggatgcatgg atacattatg caaactgctc ggtacacggt
     2641 ttgctccgat tgaagctttt ataaataaat acaaagatga tggattcata tggacattag
     2701 aaagctgtga aatgactgca cctgaaattt acaggacatt ctggcagatt cgtgaaagcg
     2761 gatggtttaa gtattgtaat gggattatat tcggtcgccc aagaggatgt gataataagc
     2821 gtgattttac attgattgat gctcttgaac aaggacttgg taatcttaat gtaccagtta
     2881 tctatgatgc tgatattgga catgttcctc cgcagataca gattgtcaat ggttctattg
     2941 gcagagtaga atttgctgat ggaaaagcaa ctattataca aagcatggag atatagcagg
     3001 agaaaatcat gttttatgat attaaagaac ggatttatga ttctgtagtt cgtgaaatca
     3061 tggcacaaag ttcttttgat caatccagag aagcaatgga tcgaaaagcg gaggaattta
     3121 atagacatgt ttcatggcag ttatatggct ggatagaaga tgaagagatt gttggtattt
     3181 gcggatttgt agtctacaga gactacatag agatccttaa tatagctgta gctcaatatg
     3241 ctcaacgtaa aggtatagga agtagaatga taacggaact gtggaataaa tacaaaataa
     3301 ctataaaagc tgaaacagat gacgatgctg tagacttcta tcggaaatgc ggtttttcaa
     3361 ctattgaaat tagtaaatac aatgttcgtc gctggacatg cgttttagaa gataagcaag
     3421 taattagtta ataaattaat gtcattcaga catattgtca gtcaattcaa tctgatttgt
     3481 taagcagata tgcatgggaa ttcctctttt ttgtgtagtt gaaatctgga aacccacact
     3541 ttcaaagtat tcaacctggt tattccatgc ccgtatatca acctgtatac caaatccagg
     3601 tttaagctta ctttttaaaa aatcaaatat ccgattaagc atttcagtct caataccctg
     3661 catctgatat tgcggtacaa ctataatatc atgtattaaa gctactgacc cgccatccca
     3721 tataatgcgg gacattccta taacttcctc tttgctgtat gcagctacaa tatatgcact
     3781 gcctgcaaga ctagcattta actgttcaag gttgattttt ctgaatccta ttgatgaacg
     3841 taaatgattc acttcttgag ccgatatttc attaacatat gttatattca ttgacactct
     3901 tccttatcac taagctgttc agttattata cagaagaaat ctgctttcca tatatctagt
     3961 gatgctgcat tctttataaa cggcaacaca tcctgttttg cctgcgagta atcaatgttg
     4021 ttgaatctct catatagcat cttctttata tcatttatgt ttatctgact attttctgaa
     4081 atattgcctg tctgtataag tcttgcagtt agatgcttaa gattaacggg tgtttttctg
     4141 gacagataaa aaatgtaatc atacaggtcc ctgcctttaa tgcggttctt ccaggacctg
     4201 catagaattg catggatctt tccagcaaaa agtgaggaga catcgtatag cgatatttca
     4261 tatggtatag gaagcagctg gtacttagtt tcaaagcatg cgtaaggagg agggtctgta
     4321 tctacttcaa cttttatctt gataagctca tttgagttaa ttgaacgagc tatcaattta
     4381 tcagagtaaa acataagtat atgctctttc gtattaccct ttaaaaatgc tgatgtaata
     4441 ttggtatcag cagatttttg ctttatagag acatcgaaat tcaagccata tgaacgaatt
     4501 tccttttcaa gtaaaggcag gtattcatta aaattaaatg atgaatcaga tgtttttaaa
     4561 gaaaaatcca gatcttcaga gaatctgtct aacccataaa atatgcgaag agctgttccg
     4621 ccgtaaaatg cagcattctt aaaaaaacct gctctgctta aaccacataa ggtaacttcc
     4681 tgtattacct gcttaatgct gtttatccta tcatgctgag tctgtgtatt atactggttc
     4741 agcatttgac ttataatact attcattttt cattctcctt ctaatgtatg cctgtaacag
     4801 cttatgattc tgtgtgcgat agttacttga gatttccaga aggtcagaca agttcattct
     4861 gaagaactca tccgaatcga tcctcaggtc atcaaaaagc agattttcta attctttttg
     4921 gttatttact ggtgataaaa tgtataactg atcacacact gccttctcag cagatgctat
     4981 ctgatatgag taaccgtttt catgatgaag attaacacct aatggatatg ctttacttgg
     5041 aacatcgcga tatgtgtaag tcccaaatgg attttcatat tgctttgctt tcttcttttt
     5101 gaatgttgct gatgtatatg tgtatacagt ttcaggtatg agcgaatgat atgctaatgc
     5161 aaattcgaaa gaaagatatg atggtccata gataatagaa gccagataat accctggtat
     5221 atttttatct gttacatata tacctcttac aacaggaatt agttcactgt tggttaccat
     5281 tcttcttatt tttgctgtcg gattaacata ttcctttagc tcttccagca acattgcagt
     5341 agtttttatc atattttcac cacctaaagc aattatagtg ctttaactgg agaaaatcaa
     5401 ggaaatattc tatcagttat atcctatcct ataaaatacg gcatctctgg atttgttttg
     5461 tttagtttaa tattaattaa ttgttgacaa ggttaaattg tatgatataa tataaacgaa
     5521 ataaaatgac ataaatcgta ttaaatatat cacaaagaag gagtatttta tgacatatat
     5581 tgaatatcta tgtaaagaga ttcgcaaaat acagcctgga gaacctattt attcaaaaca
     5641 tttatcgaaa atggttgaaa atgcttttaa catagattat aaagccgcag ctgcagcagt
     5701 tgctgttgct atgaaacgta ttattggaaa taaggtagtt cctgatttaa gaagctatca
     5761 aaaaggtatt ttttatcgaa cattagagac tgtttttggg gaaacaggaa taaataagaa
     5821 caagcttatt gctgacaagt acttagcaga taatataggg tatgaaacag gccctatcat
     5881 acttcatagg ttgggattga catcacaaat gccaaaacag cttttacttg caacaaatgc
     5941 agcaaaagac tgtatgcgag tcgacaatag acttggtgta gttattaaag ttccgaaagt
     6001 taaagtaacc gcagagaaca aagcatacct gcaaatactt gatgtactag atatgatgga
     6061 tgataatcct attgatgaaa aagaaccgca ttacattgtt gcagagcata ttaagaaaca
     6121 gaatttacag tatgacattt tacttgctct tgcagacaaa tattacaatc aaaaaactat
     6181 tctccagctt gcacgtactg ctagtgcggt agcttaaagt tatgaagctt cataatgata
     6241 gagattcatt tatagtatta ataaatgata ttcaagctaa aactggttac agagctgatg
     6301 taattgaaaa ggattattat gtagttttga tgttaaaaga gcttgcatat aaacagaata
     6361 gtggtctaca agcatacttt aaggggggaa cagcactata taaagcactg aatactacaa
     6421 atcgtttttc tgaagatatt gatgtttctg tagacacaag aggatgctgc agaagccaga
     6481 atgataaaag acttgagaat gcttcaaaga agtatatatc tttgccgaga gactccgcac
     6541 aaggcagaac aaatcgttca gaggtaattt caatttatac ttatatacct ataacagtat
     6601 atgaccaagg tgatatgctt cagagatttg gaaaagtaaa agtggaagca acatccttta
     6661 ctatcagtga acctgtcgaa gatcttgaaa tatctgcaat tatatataat caagcaacag
     6721 aggagcagaa aaatatactg agagatatct atgatgtaaa accttttagt ataaaaacaa
     6781 tatctttaga gagaattttt attgataagc tttttgctgc agagacatac actagaaaat
     6841 catcaaatag gcgcagtgca tttgatgctt caaaacatat ttatgatctt gctgttctat
     6901 cagacaaact cagaattata gaactattat caaatactga aaagatgagc agattgttag
     6961 aaatcagact tcaagaggaa tatatgcgtt tggatggtat cccagacata ttacctaatc
     7021 aattcatgtt ttttagagat gctggtacta attcggacat acgtaaggct tatgaaatta
     7081 tgcagaatca atatgttttt cgcttaaccg acaggattga gttcgataat gcactaaaaa
     7141 caatagatag aatgaggata agtttaagca aaaataaagc ttggttgctt agcaaataac
     7201 ctgagtttaa ataatttctt tattaatgca acagatcatt tattactcat ccctgtcttg
     7261 tcttataaac ccatatcgtc ccatgacaag gtttatgatg tcctgtttct tgactttcat
//
LOCUS       DAOSXM010000099         7151 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2111
            NODE_6814_length_7151_cov_10.272266, whole genome shotgun sequence.
ACCESSION   DAOSXM010000099 DAOSXM010000000
VERSION     DAOSXM010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864178
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 7151)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7151)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:24:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,261
            CDSs (total)                      :: 2,229
            Genes (coding)                    :: 2,218
            CDSs (with protein)               :: 2,218
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 3 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7151
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6814_length_7151_cov_10.272266"
                     /isolate="AMR_MDS_2111"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(475..2523)
                     /locus_tag="PLQ68_09770"
     CDS             complement(475..2523)
                     /locus_tag="PLQ68_09770"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HXK72982.1"
                     /translation="MFKIQKDKKEKREKKKRKSEGLEEKSMKIGTKLVLMLSIIMTAL
                     MALAFVLVIKVSSDNANEAAINNISSVANNNAGMIKASLEVPLDTARALANSFQAYTD
                     IKAEDRRRNFNTILKNVLKENEEYVGVWSCWEPNALDGMDLQFTATEGTDTTGRFISS
                     WQRVNGNIILTPLVDYATYGPGDYYLKAKSTRGEIILDPYFYEMDGKDILLTTVTVPI
                     KNNVGKVIGAVGIDIALSELQKMGFDKGGYYSANTYVISNNGTYVIAPDESIVGTVIE
                     NQQIDEEKDLLSIISTGEKYQTEKKSVVFSDMVSACYVPITLGSTTTAWSLYVEVDKT
                     EVAESTKDIIVLMSGIFAALLLTVIVTLLIIIQFIVSKPIKETADFAKALSLGRLDAT
                     VKIKSKDEIGQLKTLLDNDMRNTFMKIEKARVDIDNASKQVAISSQQLSEGSQSISSG
                     STQQASSLQQLSASVEQIAEQSNYNADISNKANKLSQNAKESASQGNDLMKNMQVAMS
                     DINKSSNNISKIIKVIDDIAFQTNLLALNAAVEAARAGAHGKGFAVVAQEVRNLAARS
                     AKAASETTELIEGSIKNVNRGTEISNMTEQSLKDIVENIDKAYELVEQISKSSQEQSS
                     SVNQINNALNELSKVVQSNSASAEQTAASSEELSSQAALLKDMVSMLKISEEEKAENE
                     "
     gene            complement(2878..3312)
                     /locus_tag="PLQ68_09775"
     CDS             complement(2878..3312)
                     /locus_tag="PLQ68_09775"
                     /inference="COORDINATES: protein motif:HMM:NF022896.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3021 family protein"
                     /protein_id="HXK72983.1"
                     /translation="MSFKVFIKRCFMEYFIITTCVTAAIAILGLCIDPSARFGYESYF
                     SPLIFGLVSILPSGVTFSRKELSLKQTIIRKILHFFFLVLMLVLFVFWTGLLKGFNEA
                     VSFVFSVFIVYVIVNIVSWHLNKKEATKVNKLLKHFQEDKVR"
     gene            complement(3309..3755)
                     /locus_tag="PLQ68_09780"
     CDS             complement(3309..3755)
                     /locus_tag="PLQ68_09780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020815851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HXK72984.1"
                     /translation="MKVSLKKIDRVLEEHAIICCYEVNDDIKSIVKYIKSTGTLISGY
                     DEDRQKQVVLNDIFYIEAVDNKVFAYTSNNVYELKCKLYEFAEQYDKRHFFRCSKSCI
                     VNLMKIESVQPFLNGRLSAVMLNKEELIISRQYVPLLKKTLLGEEI"
     gene            complement(3918..5222)
                     /locus_tag="PLQ68_09785"
     CDS             complement(3918..5222)
                     /locus_tag="PLQ68_09785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020815852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72985.1"
                     /translation="MLGIITLIVALSAQIALLVYQMITKNHQQKVKNIMRLTTFGLFT
                     LLLSVNVYWWGFRWTGLFILLLVLALISIISLIRKRQTVREFKPAKAILICINICILL
                     TFSILPGIIFPQFNPIEVTGEYDVQTASVTLTDEDRTESYSEESEKRKITIQFWYPNL
                     KDNDEKLPLIIFSHGSFGYRGSNLSTFLELASNGYVVCSIDHSYHAFFAKHMDKTMTI
                     VDVNFFNDAVSITNNEYEAEYTYNLLHEWLDLRKADMSFVLNEITNNESGKIPDNIYS
                     IIDNERIGLTGHSLGGATAASLGRELDIVDAVIVIDGTMIGEGIDFKDNEEVLDLNAY
                     PVPLLNLYNEDHYTDAMSQGTKYNNISASTNAVEAYDVVIRKSGHLNFTDLPMFSPAL
                     ASLLGTGDVDNRYCIETMNQLVLDFFNYTLKQQGELNIKTEY"
     gene            5446..5934
                     /locus_tag="PLQ68_09790"
     CDS             5446..5934
                     /locus_tag="PLQ68_09790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012060087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HXK72986.1"
                     /translation="MIREMKNEDWNDVSRIYQQGIEAKNATFETMLPEYKQWDATHLK
                     ECRLVSTDQEGNVNGWIALSPTSSRLVYKGVAEVSVYVDKQSIRKQIGYSLMQRLIEL
                     SEQYGIWTLYSSIIASNQPSIYFHTKCGFRTVGIREKIAKDADGIWTDTVIMERRSKK
                     VW"
     gene            complement(5958..6821)
                     /locus_tag="PLQ68_09795"
     CDS             complement(5958..6821)
                     /locus_tag="PLQ68_09795"
                     /inference="COORDINATES: protein motif:HMM:NF021537.2"
                     /note="predicted Zn-dependent protease with a strongly
                     conserved HExxH motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2268 domain-containing putative Zn-dependent
                     protease"
                     /protein_id="HXK72987.1"
                     /translation="MDILVLDTYRIYKEMLLLSEEQREDFFNKSIVEPFDVLFKLTSM
                     PRKPEMLSCLPLSGQESITFLMFECLKENDIWEKAKTSIEISIHSFEKKGIKLFDNLI
                     VAILPGDKQLLSLSEGYTGIGSIPGYIMLVIAPDENNVKKFPACVAHEFHHNVLFNNF
                     IWDYSSISLSQYLAIEGLAESFSEDLYGSETVGPWVSSTIGEMLENAKKIIGINLNVK
                     GFMNVNRYMYGKHPMFAGSESVDLPYCAGYAVGYHAVQKYVKDRNTDVIQATIDFINK
                     IDIVKESGYFE"
BASE COUNT         2328 a         1285 c          991 g         2547 t
ORIGIN      
        1 gaactaccac aatgacagat ataatgagta atgcgcttat cttcttacct actccgaaat
       61 tcttaaataa ttttttcaat ttatccccct ctattttttt gtatcttaat actactaagc
      121 acattctgat tatatttttg tgtttttaag tattagatat taatttttat gtcaatacat
      181 gatgtaaata aggcatttgt gaattgtttt atgctttagc aattcatgta tatccattta
      241 actaatatat cggcagcttt caatatttgt ttaattttta acaatgtttt ttagaataaa
      301 caatatttac taaaatttct caaaccaaaa gaaaaacaat gattttaatt tgatactttc
      361 aatgtaacaa ttcttatgca aatgcaaata aatatacata agcaaaaacc ctcattgcgg
      421 ggagggttta tgctttgaaa aatacatata aatataacag ggtgttatgt tagattactc
      481 gttttcagct ttttcttctt ctgatatctt caacatagat accatgtctt taagtaaagc
      541 tgcctgactc gataattcct cgctagatgc agcagtctgc tctgcagatg cagaattact
      601 ctgtactact ttggatagct cattaagagc attgttaatc tgattaacag aacttgactg
      661 ttcctgagat gatttggaaa tctgttctac taattcatat gctttatcaa tattctcaac
      721 tatgtctttc aatgattgct cagtcatatt tgagatttct gtacccctat tgacattctt
      781 tattgaaccc tctattaatt ctgttgtctc acttgcagct tttgcgctcc ttgctgcaag
      841 atttctgacc tcctgggcaa caactgcaaa accttttccg tgcgcacctg ctcttgctgc
      901 ttctacagct gcattaagcg ctagtagatt tgtctggaat gctatatcat caattacctt
      961 tataatcttt gatatattat tagatgattt attaatatca ctcatggcaa cttgcatgtt
     1021 tttcataaga tcatttcctt gtgaagccga ttccttcgcg ttttgagata atttattagc
     1081 cttgtttgaa atatcagcat tgtagtttga ctgttctgct atttgttcta ctgaagctga
     1141 taattgttgc aaagaactcg cctgttgggt agaaccagat gagattgact ggcttccctc
     1201 agacaactgt tgtgaactta tagctacctg ctttgaagca ttatcaatat caactctggc
     1261 cttctcgatt ttcataaacg tattacgcat atcattatct aaaagtgtct taagttgccc
     1321 aatttcatct ttcgatttaa tctttacagt tgcatctagc cttcccaaag ataatgcctt
     1381 tgcaaaatca gctgtttctt ttatgggttt gctaacaata aactggataa ttataagtaa
     1441 tgtcactata actgtcagca ataaagctgc aaatattcct gacatgagaa ctataatatc
     1501 cttagtagac tctgctactt cagttttatc aacttcaaca taaagtgacc aggcagttgt
     1561 tgtacttcca agggtaattg gaacataaca agcactcacc atatctgaaa aaacaactga
     1621 ctttttttct gtttgatatt tctcgccggt tgaaataatt gataataaat ctttttcttc
     1681 atctatttgc tgattttcga ttacagttcc aacaatactt tcatcaggag caattacata
     1741 tgtaccatta tttgatatta cataagtatt tgcactgtaa taacctcctt tgtcaaaacc
     1801 cattttctga agttcagata atgcgatatc tataccaact gcaccaatta ctttacctac
     1861 attgtttttt attggcaccg taacagttgt aagtagaata tccttaccat ccatttcata
     1921 aaaatatgga tctagaatta tttctcctct tgtggatttt gcttttaggt aataatcacc
     1981 aggaccataa gttgcataat caactaaagg tgtcagtata atatttccgt taaccctctg
     2041 ccaagaagat ataaatcttc ctgttgtatc tgtaccttct gttgcagtaa actgaaggtc
     2101 cattccatca agtgcattag gttcccagca ggaccagact cccacatatt cctcattttc
     2161 tttgagtaca tttttcaata ttgtattaaa gttacgtctt ctgtcttctg cttttatatc
     2221 agtataagcc tgaaagctgt ttgctaaggc tcttgctgta tctagcggta cttccaatga
     2281 tgctttaatc atacctgcat tattattagc aacagatgag atattgttaa tagctgcttc
     2341 attggcatta tcagaagaaa ctttgataac caatacaaat gcaagagcca tcaaagctgt
     2401 cattattatt gacagcatta aaactaattt cgtgcctatt ttcatacttt tttcttctaa
     2461 accttcagat tttcttttct tcttttccct tttttctttt ttgtcctttt gaattttaaa
     2521 catttccatg cccctttata tatgatactt gagaattatt cgtcctatgt ttttttcatc
     2581 aataaaacat tcttatcata tcaatttact atatcagcaa tctctaactg agattaaatc
     2641 tcaattaaat attatttata agtagctcct aatatattat cggttaattt ttaaaaatat
     2701 tcagttttat tttataaata ttttattttt ccctaaaaaa tattttattt ttgttattgg
     2761 taaacattga cattaatata ttatttatca aactctatta ttcaaatgat tgtctcataa
     2821 acttggaatt gatatattaa aaatacatca gctataacat gcaaagacta tatttaatta
     2881 tcttactttg tcctcttgaa aatgcttaag aagtttattt accttggttg cttctttctt
     2941 attcaaatgc cagcttacaa tgtttacaat cacatataca ataaatactg aaaaaacaaa
     3001 tgaaacagct tcgttaaatc ctttaagaag tccggtccag aaaacaaata aaacaagcat
     3061 tagcacaaga aagaagaaat gcagtatttt tcttattatt gtctgcttta gtgaaagctc
     3121 tttacgggaa aatgtcactc cagaaggtaa tatgcttact aatccaaaaa ttaaaggtga
     3181 aaaatatgat tcatatccaa atctggctga agggtcaata cacaaaccta agattgcaat
     3241 agcagctgtt acacatgttg ttattatgaa atattccata aaacatcttt ttataaacac
     3301 tttaaaactc atatttcttc acctaataat gtttttttca ataatggtac atactgtctt
     3361 gatataatta gttcctcttt gttgagcatt actgcagata atcttccatt taaaaaaggc
     3421 tgcacactct ctattttcat caaatttaca atacaggatt tagagcatct gaagaaatgt
     3481 cttttatcat actgttcagc aaattcatat aacttacact ttaactcata tacattatta
     3541 cttgtatatg caaatacttt attatctaca gcttctatgt agaaaatatc attaagaaca
     3601 acttgttttt gtctgtcttc atcatatccg ctaatcagag tgcctgtaga ttttatgtat
     3661 ttaactatac tcttgatatc atcatttact tcataacagc atataattgc atgctcctcg
     3721 agaactctat ctatcttttt taatgaaact ttcaaatcat ataccttcgc aacttggaat
     3781 taacaagaaa ttaataccat attagactgg ataatttttg aacctatcct gcccgaaaga
     3841 gtatagcagt tttttactca acttacaatt agcattaccc tatgtgagaa aacagtcatt
     3901 ctcacatagg gattatccta atattctgtt tttatattca attcgccttg ctgctttaaa
     3961 gtgtagttaa agaaatcaag cactaactgg ttcatagttt caatacaata cctattgtca
     4021 acatctcctg ttccaagaag gcttgccaat gcaggagaaa acataggtag atcagtaaaa
     4081 ttaagatgac cggattttct tataaccaca tcatatgctt ctacagcatt tgtgcttgca
     4141 gaaatattat tgtattttgt tccctgactc attgcatctg tgtaatgatc ttcattataa
     4201 agattcaata atggaacagg ataagcattt aagtccaaaa cttcttcatt atctttaaaa
     4261 tcaataccct caccaatcat agtaccatca attacaatga cagcatctac tatatccagc
     4321 tcccttccaa gtgaagctgc tgtagctcct ccaagtgaat gccctgtaag accaattctt
     4381 tcattatcaa ttatagaata tatattatcc ggaatctttc cagattcatt attagtaatc
     4441 tcgtttaaaa caaagctcat atccgcttta cgtaaatcca gccactcatg taataaatta
     4501 tatgtatatt ctgcttcata ctcattgttt gtaatgctta cggcatcatt aaaaaaattc
     4561 acatcaacaa ttgtcattgt tttatccata tgttttgcaa agaatgcatg ataactgtga
     4621 tctatactgc agacaacata tccattgctt gcaagttcta aaaatgttga cagattgctg
     4681 ccccgatatc caaatgaacc atgcgagaat attattaaag gcagcttttc atcattatcc
     4741 ttaaggtttg gataccaaaa ctgtattgta atttttcttt tttctgattc ttctgaatat
     4801 gattctgttc tgtcttcatc cgttaaagta acactagctg tctgaacatc atattctcca
     4861 gttacttcaa ttgggttaaa ctgaggaaat attattccag gcagtattga aaaggtaagc
     4921 aatatgcaga tatttataca tatcagtatt gcttttgcag gtttgaactc cctaacagtc
     4981 tgccttttcc taataagaga tattatactt attaaagcaa gaactaaaag aagtataaat
     5041 aaccctgtcc agcgaaatcc ccaccagtaa acattaactg ataagagcag tgtgaataag
     5101 ccaaatgttg taagcctcat tatatttttt accttttgct gatgattctt tgtaatcatc
     5161 tgataaacca atagagcgat ttgtgcgcta agcgcaacga ttagtgtaat aattccaagc
     5221 atatttttta attcctttct gacttttgtc ttggcaagta tagctagtac agatatatat
     5281 gacaacgctt ttagaggtat gttgcagaaa atcgaggtaa gttacatatt catcaatata
     5341 tcttcctgac attccatttt ttaattactt tgtattttta aaatctttct tttgatgtat
     5401 aattccgatt agaatttatg cttaagcagg ataggtgata gcgatatgat tagagaaatg
     5461 aaaaatgaag actggaatga tgtaagcaga atatatcagc aaggcataga agcaaaaaat
     5521 gctacatttg aaacaatgct tcctgaatac aagcaatggg atgcaactca tttaaaagag
     5581 tgcagactag tttcaactga ccaagaaggc aatgtaaacg gctggatagc tctaagtccc
     5641 acaagttcaa gactggtata taaaggagtt gcagaagtca gtgtttatgt tgataaacag
     5701 tctataagaa agcagatagg atacagcctg atgcaaagac taattgaatt atctgaacaa
     5761 tatggtatct ggactctgta ttcatctata atcgcatcaa atcagccaag catctatttt
     5821 cataccaagt gcggattcag aacagtaggt ataagagaaa aaatagcaaa agatgcggat
     5881 ggtatatgga cagataccgt tatcatggaa agacgaagca aaaaagtttg gtaaattcga
     5941 attaataact gttgaattta ttcaaaatat cccgattcct taacaatatc tattttgttt
     6001 atgaaatcta ttgttgcctg tataacatca gtatttcgat ctttaacata tttttgaacc
     6061 gcatgatagc cgacagcata tcccgcacag taaggtaaat caaccgattc acttcctgcg
     6121 aacattggat gctttccgta catgtatctg tttacattca taaatccttt gacattaaga
     6181 ttaatcccaa taatcttttt tgcattttca agcatttcac ctattgtaga acttacccat
     6241 ggcccgactg tttcgctgcc atacaaatct tctgaaaaac tctcagcaag cccttcaatg
     6301 gcaagatact ggcttaaaga aatagacgaa taatcccata tgaagttatt gaacaagaca
     6361 ttatgatgaa attcatgtgc aacacatgct ggaaattttt tcacattatt ttcatctggc
     6421 gctattacaa gcataatgta ccctggaata gaccctattc cggtataacc ttcacttaaa
     6481 gacaaaagct gtttgtctcc tggtaatata gcaacaatta aattatcaaa taacttaatt
     6541 ccttttttct caaaagaatg aatacttatt tcaatagaag tttttgcttt ctcccagata
     6601 tcattttctt tcagacattc aaacatcaaa aatgtaatac tctcctgccc agataaaggc
     6661 agacaagata acatttcagg ttttcttggc attgatgtta gtttaaataa cacatcgaag
     6721 ggttcaacta tactcttatt aaaaaaatcc tctctttgtt cctcgctaag taaaagcatc
     6781 tctttatata ttctatatgt gtccagaacc aaaatatcca taaaacagtc tccttcctta
     6841 aatatatata taactataaa tcattacgct acggcagggt caataatatt tttatgttgt
     6901 ttaactaata aaacaacaaa ttaatttatt tgctaataca cgataaatag tagaaatata
     6961 cggctataag tattaaactt ttaattaact gctaaatatg gaaggaattc aggataaatg
     7021 agtaaaaaag ggataaagct aaatatatta ataattataa ttattctttg tatgttattt
     7081 ttatcaagct gtgaaaatat gattacattt tccggaactt gcgtttcaat tggaaagaaa
     7141 tttgaactag a
//
LOCUS       DAOSXM010000100         7079 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2111
            NODE_6895_length_7079_cov_10.071327, whole genome shotgun sequence.
ACCESSION   DAOSXM010000100 DAOSXM010000000
VERSION     DAOSXM010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864178
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 7079)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7079)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:24:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,261
            CDSs (total)                      :: 2,229
            Genes (coding)                    :: 2,218
            CDSs (with protein)               :: 2,218
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 3 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7079
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6895_length_7079_cov_10.071327"
                     /isolate="AMR_MDS_2111"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            219..1133
                     /locus_tag="PLQ68_09800"
     CDS             219..1133
                     /locus_tag="PLQ68_09800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019226275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HXK72988.1"
                     /translation="MNKEKRRNIVGWTGVAITTIFSSIWAYWGSIENFHEGWYSVSIW
                     ENLFMLLFQYLLFTVIFVLLGVITLKWKIAGIVLHILLAGFCIWFFSGASFLVIGLMI
                     VIPIIGLGLLYFFGKPEPKKWAYRSLIFIPLIIIIAVFIPMSIKVSKRINDYDFGVRI
                     VEGNEITLIWAPRGLGWPDSGTTWHEAMDICRYLSEDGTKIMDEVQDIWRLPTVDEAV
                     RSMLLHGENVNGIWYPDDQKAVYDKTPDKESPLWDVHSQVIYYWTCETAKDNEDRAYI
                     IVYHGGVNSKMKIDGQSYLSFRAVKNID"
     gene            1215..1412
                     /locus_tag="PLQ68_09805"
     CDS             1215..1412
                     /locus_tag="PLQ68_09805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72989.1"
                     /translation="MIKLPRRDGTGPSGSVRGTGFGRQSNQQSQNYCICPKCGTRIAH
                     TRGTPCNAISCPKCGTLMTRE"
     gene            1429..2355
                     /locus_tag="PLQ68_09810"
     CDS             1429..2355
                     /locus_tag="PLQ68_09810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010964929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HXK72990.1"
                     /translation="MVEYKYIYGPVPSRRLGLSLGISPIPKKTCNYSCIYCQLGRTNH
                     MTNTKKMFYPVNEIVHELKECINNGVSFDVVTIVGEGEPTLYLGLGELIVNIKNLIDK
                     PVAVITNGALLYDKSVQDDLKEADIVLPTLDAYNEELFKKINRPHKSLEYCCILSGLK
                     EFSRIYKGQIWIEIMLMKNVNDSDYDLEKYKEILKTIRYDKLYINTPIRPPAESNVYA
                     SDHERVRYAADYLNGLSIDLLASTGFQSSIKDDYEAILSIIKRHPMNQYEIESFLNSR
                     NVKNIKDLLRKLNSDVIVNVLDYKGYKTYRLK"
     gene            2393..3115
                     /locus_tag="PLQ68_09815"
     CDS             2393..3115
                     /locus_tag="PLQ68_09815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008012265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HXK72991.1"
                     /translation="MNDSNVFLMISIFAISALIVNSIGIYVIYRNMKWVEKYKEYFMC
                     FAAGVLISAPLISAFPEAIEKNHNAGFAALIGFVFMLFSNKLIKHHTKQEELAFGITA
                     IQGIGIHSLLDGIIYTVTFSASIFTGLVSGIGLVIHEFTEGVITFSVLVKGGIKTKKA
                     ILYAFLVAGLTTPVGAFVAYPLVKRLSDSTLGLALGFVVGVLIYISAAHLLPEAKAHE
                     KKHSMVALCLGIVLSLAMSFLH"
     gene            3247..3555
                     /locus_tag="PLQ68_09820"
     CDS             3247..3555
                     /locus_tag="PLQ68_09820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HXK72992.1"
                     /translation="MDNKEINNERYMSSVKVGPKGQIVIPKEVRDMFKIKSGDSLILL
                     ADSQKGIAIERMSVFHNIADAILEGKAKEMYPEHTESDSVNFAKSIKRLETTEDERKK
                     "
     gene            3552..4286
                     /locus_tag="PLQ68_09825"
     CDS             3552..4286
                     /locus_tag="PLQ68_09825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXK72993.1"
                     /translation="MIAIKTDNLTKKFKNTTAVDNLNLEINQNELFALLGVNGAGKTT
                     TIKMLSCLINPTEGDAFVFDISISKEPNKVKPLMNVSPQETAVAPNLTVKENLELIGG
                     IYGMDKKAASEKAQNMINLFGLEEIQNNRAKILSGGYQRRLSIAMALISEPKLLFLDE
                     PTLGLDVLARRELWKVIKNLKKDITIILTTHYLEEAVALSDRIGIMVKGKLKIVGTAE
                     KIIQTMNTDNFEDAFIAISEDIGEKA"
     gene            4283..5020
                     /locus_tag="PLQ68_09830"
     CDS             4283..5020
                     /locus_tag="PLQ68_09830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXK72994.1"
                     /translation="MKMSAFALRNTKEILRDKLNLAFGLGFPIIVLLLLSAIQANIPV
                     ELFAIDHLTPGICVFGLSFISLFSGMLIAKDRTSSFMLRLYISPMKSSDFILGYTLPL
                     LPMAMMQIIICIIAAMALGLSINTNILLTIVVLIPAAILFIAIGLLCGSIFNEKQVSG
                     ICGALLTNLAAWLSGTWFDLDLIGGTFKTIANLLPFYHAVEAGRAAMSGSFDMIIPHL
                     LWVIGYGAAIMGFAVYAFAKKAKSDNK"
     gene            5044..5493
                     /locus_tag="PLQ68_09835"
     CDS             5044..5493
                     /locus_tag="PLQ68_09835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014357648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HXK72995.1"
                     /translation="MSEYDLIAPCGMNCSLCVSYQFGKFDFNKKGFSKKYCPGCIPRG
                     KNCTYMAHHCDLLGKGIIRFCTDCKDFPCKYLKYLDKRYSTKYKMSMIDNLHYISNNG
                     IDKFLLKEEEKWKCNICNNLKCCHNGLCLFCEIDKLATNKKYKRYDL"
     gene            5490..5708
                     /locus_tag="PLQ68_09840"
     CDS             5490..5708
                     /locus_tag="PLQ68_09840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72996.1"
                     /translation="MKKENWYKFLFAVSGLLVVGFIIRVIADYIQYDPIATSFPFYAT
                     LLLRSVEFILPCIITFVIAIILKKKYSN"
     gene            complement(5787..>7079)
                     /locus_tag="PLQ68_09845"
     CDS             complement(5787..>7079)
                     /locus_tag="PLQ68_09845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK72997.1"
                     /translation="WIYQKYFNRCAGKGNVPIKFNGSIFVTYPFESDNGSITWDYRRW
                     GGAYWIQNTRHIYWNMLLSGDFEGMKPLYDMLTNLIPISKHRCRQYFSHDGMLLPETF
                     TIGGLYCASNYGIPQEDGKRYAKGTWLHQPGQIANHYIRWHYNGMLELAYLILLGAKF
                     ADDEKYKDIAYNFSREVLTFFYKHFDTLDGKILMKPVSSLETYQDCVNDLPDIAGLDA
                     LTDELLAQEDIYEELKIICEKVRDILVDYPKDDNKLMPCQNKIDTKRRNSENPELYSI
                     FPFYRHTLINKEDELFSFAVNAFNERSEKAGLGWSQDCIQSALLGLVDQTEQILIKQA
                     LNTDERYSFSGIYGPNYDETPDQDHANGLSIGLIHSLILSTREKNMLFLAWPEKWNVS
                     FRLPLYSSKSVSVIYEDGKIKHIEFEDKLDEIRTVINL"
BASE COUNT         2408 a         1137 c         1280 g         2254 t
ORIGIN      
        1 gatttttttt actatttcat caattttttt cttcaagtcc attatcgctc tccttaaaat
       61 tttattacta actgcatata ttataccacg aatacctaga tataaagtct ttaattgata
      121 cattgtatta atttacactc actgctcctt tttataaaaa tagtaataat gaatatcttg
      181 aagtgatata attaaaataa attcatagag gtaggataat gaacaaggaa aaaagaagaa
      241 acatcgtagg ctggactggt gtagcaatta cgactatatt ctcaagtata tgggcgtact
      301 ggggttcaat agaaaatttc cacgaaggct ggtactccgt ttcaatatgg gaaaaccttt
      361 tcatgttact tttccaatac ctgttattta cagtcatatt cgttctactg ggagtaataa
      421 ctcttaaatg gaaaattgca ggaatagtac ttcacatttt gcttgcaggt ttttgtatat
      481 ggtttttttc tggagccagc ttccttgtta taggattaat gatagtcata cctattatag
      541 ggcttggact tctttacttt tttggtaaac ccgaacctaa aaaatgggca taccgatcat
      601 taatttttat tccgcttatc atcatcatag ctgtatttat accaatgtct ataaaagtat
      661 ctaaaaggat aaatgattat gattttggag taagaatagt tgaaggaaat gaaataactc
      721 taatctgggc tccaagaggc ctgggatggc cagacagtgg tacaacctgg catgaagcta
      781 tggatatatg cagatatctt tcagaagacg gaactaaaat aatggatgaa gtccaggata
      841 tatggagatt gcctactgtt gatgaagcag taagatcaat gttgctccac ggcgagaatg
      901 taaacggtat atggtatcct gatgaccaaa aggcagtgta tgataaaaca ccagataagg
      961 aatcaccttt gtgggatgtc cactctcagg ttatatacta ctggacttgt gaaactgcca
     1021 aagataatga ggacagagca tatattatcg tatatcatgg aggcgtaaat agcaaaatga
     1081 aaatcgatgg acagagctac ttaagcttca gagctgtaaa gaatattgat taattttaaa
     1141 gtagttagca atggcttatt gacttagcga aaatgaatta tataattgaa tataggtaag
     1201 taatgccaat ggaggtgatt aaattgccaa gaagagatgg aaccggacct tccgggtcag
     1261 ttagaggtac aggatttgga agacagtcaa atcaacaatc acaaaattac tgcatttgcc
     1321 caaaatgcgg aacaagaatc gcacatacaa gaggtacgcc ctgtaacgca ataagctgtc
     1381 ctaaatgcgg caccttgatg actagagaat aaatttcagg agttaactgt ggttgaatat
     1441 aaatatatct atggacctgt gccttcaaga agactagggc tatcactagg gataagccct
     1501 attcctaaaa aaacatgtaa ttattcctgc atatactgtc aacttggaag aaccaatcat
     1561 atgacgaata caaaaaaaat gttctaccct gtaaatgaaa ttgttcatga gttgaaagag
     1621 tgtataaata atggagtgtc ttttgatgtt gtaactatag taggagaagg tgaacctact
     1681 ttgtatttag gacttggtga gttaatagtt aatatcaaga acctgataga taaacctgtt
     1741 gcagtcataa caaatggtgc tttgctttac gataaaagcg ttcaggatga tttaaaagaa
     1801 gcagacatag tattacctac actggatgct tataacgagg aattattcaa aaaaataaac
     1861 agacctcata aaagtcttga gtattgttgt attttatcag gattgaagga gttttcccgt
     1921 atatataaag gtcagatctg gattgaaata atgctgatga agaatgttaa tgattctgat
     1981 tatgatttag aaaagtacaa agaaatatta aaaacaatcc ggtacgataa attatatata
     2041 aatactccga ttagaccccc agcagaaagc aatgtatatg cttcagatca tgaaagagtg
     2101 agatatgctg ctgattattt aaatggtttg tcaattgact tactggcatc tacaggcttt
     2161 caaagtagca ttaaagatga ttatgaagca atattgagca taataaaaag gcatcctatg
     2221 aaccagtatg aaatagaaag ttttttaaac agcagaaatg taaaaaacat aaaagacctc
     2281 ttgcgtaagc ttaattctga tgttattgta aatgtgctgg actataaggg ttataaaaca
     2341 tataggttaa aatagaaata gaattatatt aagttttgca gagaggaatg ctatgaacga
     2401 cagtaacgta tttttaatga tatcaatatt cgctatcagt gcattaattg taaattcaat
     2461 tggtatttat gtaatttaca gaaacatgaa atgggtagag aaatacaagg aatactttat
     2521 gtgttttgct gctggtgtcc taatatccgc gcctctcatt tcagctttcc ctgaagcaat
     2581 tgagaaaaac cataatgcag gctttgctgc tcttataggt tttgtattta tgcttttcag
     2641 caacaaatta attaaacatc atacaaagca agaagagtta gcctttggta taaccgcaat
     2701 tcagggtata ggcattcact cgcttcttga tggaattata tatactgtta ctttcagcgc
     2761 aagtatcttt accggtcttg tttcaggtat cggtttggta atacatgaat ttactgaagg
     2821 tgtaattact ttttctgttc ttgttaaggg tggcataaaa actaaaaaag ctatacttta
     2881 tgcctttttg gtagcgggcc ttacaacacc tgtaggtgct tttgtcgcat accctttagt
     2941 taaaagactc agtgattcca cgctaggtct tgcgttaggc tttgtagtcg gagttctcat
     3001 ttatatctct gctgcacatt tacttccaga agcaaaagct catgaaaaga agcattcaat
     3061 ggttgcctta tgcttgggaa ttgttttatc attagcaatg tcctttttac attaaataca
     3121 aaaatatgga ttaagtcatg acacttgttt ttaagcaagt gttttttata tgtattgacc
     3181 tttctgtata atttgttata cttgtattac ttgttatact ataacaataa ttatggagaa
     3241 gagtttatgg ataataaaga aataaataat gaaagatata tgagttctgt aaaagtaggc
     3301 cctaaaggtc agatagtaat acctaaggaa gtcagagata tgtttaaaat caaatctggt
     3361 gattctctta tcctattagc ggactcacaa aaaggaatag cgatagaaag aatgtctgta
     3421 tttcataata tagctgatgc aatcctcgaa ggaaaagcta aagaaatgta tccagaacac
     3481 actgaaagtg atagtgttaa ttttgctaaa agtattaaaa gactggaaac tacagaagat
     3541 gagaggaaaa aatgatagcg attaagactg ataatttaac aaagaaattt aagaatacta
     3601 cagcagttga taatcttaat cttgaaataa atcaaaatga actgtttgct ctcttaggag
     3661 ttaatggtgc aggaaaaacc actactatca agatgttatc ctgccttata aaccctacag
     3721 aaggagacgc atttgtattt gacataagca tttcaaaaga accaaacaaa gtgaaacctc
     3781 ttatgaatgt atcaccccag gaaacagcag tcgctccaaa tcttactgta aaagaaaacc
     3841 ttgaactgat tggtggaata tatggaatgg ataagaaagc agctagcgaa aaagcgcaga
     3901 atatgatcaa tttatttgga cttgaagaaa tacagaacaa ccgcgctaaa atattgtctg
     3961 gaggttatca gagacgactt agcatagcaa tggctttaat atcagaacct aaacttctat
     4021 ttcttgacga accgactttg ggtttagatg tacttgcaag aagagaattg tggaaagtaa
     4081 ttaaaaactt aaaaaaagat ataaccataa tccttactac ccattatcta gaagaagcag
     4141 tcgctttatc agacaggata gggattatgg taaaaggtaa attaaagata gttggtactg
     4201 cagaaaagat aatacagaca atgaatactg ataattttga agatgcattt atagcaatct
     4261 cagaagatat aggtgagaaa gcatgaagat gagcgcattc gccttaagaa acacaaaaga
     4321 aatattaaga gacaaactga atcttgcatt cggtttgggt tttccaatta ttgtcctact
     4381 ccttttatct gccattcagg ctaatattcc tgttgaactt tttgcaattg accaccttac
     4441 tcctggaata tgcgtattcg gtctgtcttt catttccctt ttttctggaa tgctgattgc
     4501 taaagacaga acaagctcat ttatgttgag gttatacatt tcaccaatga aatcgtcaga
     4561 tttcatactt gggtataccc ttcctttact gccaatggca atgatgcaga taattatttg
     4621 tattattgca gccatggcac tgggactttc tattaataca aatattcttc ttactattgt
     4681 tgtactaata ccagctgcaa tactgtttat tgcaataggc ttgttatgtg gaagtatttt
     4741 caatgaaaag caggttagcg gtatctgtgg agcattactc actaatctcg cagcctggct
     4801 ttcaggaaca tggttcgatc ttgaccttat aggaggaact ttcaaaacaa tcgctaacct
     4861 actccccttt taccatgcag ttgaggcagg aagggctgca atgagtggca gttttgatat
     4921 gatcatacct catttattgt gggttatcgg ttatggagca gctataatgg gatttgcagt
     4981 atatgcattt gcaaagaagg ctaaaagtga taacaagtaa tatgatcaaa aaaggacagg
     5041 aaaatgagtg aatatgattt aattgcacca tgtggaatga attgcagtct atgtgtgtcg
     5101 tatcaatttg gaaaattcga tttcaataaa aagggtttta gcaaaaagta ctgtccaggt
     5161 tgcataccaa gaggaaaaaa ctgtacatat atggctcatc actgcgacct tttaggaaaa
     5221 ggaataataa gattctgcac agactgcaaa gattttccat gcaaatatct taaatatctt
     5281 gataagagat acagtacgaa atataagatg agtatgattg ataaccttca ctacatatca
     5341 aataatggta ttgataagtt tcttttaaaa gaagaagaaa aatggaagtg taatatatgc
     5401 aataatttaa aatgctgcca taatgggttg tgtttgttct gcgaaattga taaactagca
     5461 accaataaaa agtataagag gtatgattta tgaaaaaaga aaattggtat aagttcctat
     5521 ttgcagttag cggtttgctt gtggtgggat ttatcataag agtaatagca gactacatac
     5581 aatacgatcc tatagcaact tcctttccct tttatgcaac ccttcttctt agatcagtag
     5641 aatttatact cccatgtata ataacttttg ttattgctat tatccttaag aaaaagtata
     5701 gtaactaaca agaatcagtt actcatatgt aaatcactaa ttgctttaaa gcaaatacaa
     5761 tataagagga ataatttatc tgtaagttat aaattaataa ctgtccttat ttcatctaat
     5821 ttatcttcaa attcgatatg cttaatttta ccatcttcat atatgacact aacagacttt
     5881 gatgaataaa gcggcagcct aaaagacaca ttccattttt ctggccatgc caaaaagagc
     5941 atattcttct ctcttgttga aagtattaaa gaatgaataa gccctatact caacccgttt
     6001 gcatggtcct gatctggtgt ttcatcgtaa ttggggccat atattccact aaatgaatac
     6061 ctttcatctg tatttaaggc ctgtttaatc agtatctgct ctgtttgatc aacaagtcct
     6121 agtagtgctg actgtataca atcctgagac cagcccaatc ctgctttttc agatctttca
     6181 ttaaaagcat ttactgcaaa actgaatagc tcatcttctt tgtttatcaa tgtatgcctg
     6241 taaaatggga atatgctgta caactcagga ttttcactat ttcttctttt agtatcaatt
     6301 ttattctgac aaggcataag cttattatca tctttaggat aatccactaa aatatctctt
     6361 accttttcac aaatgatctt cagttcttcg taaatatcct cttgagctaa aagttcatca
     6421 gtcaaagcat ccaatcccgc tatatcaggc aggtcattta cgcagtcctg ataagtctcc
     6481 agcgaagaaa ccggtttcat taatatttta ccgtctaaag tatcaaagtg cttgtaaaag
     6541 aatgtaagta cttctcttga aaaattatat gctatatctt tgtatttttc gtcatcagca
     6601 aatttcgctc caagtaaaat aagataagct aactccagca taccattata gtgccatcgg
     6661 atataatgat ttgctatctg tcctggctga tgaagccatg ttccctttgc gtatctttta
     6721 ccgtcttcct gaggtattcc atagttagaa gcgcaataaa gcccaccaat tgtaaatgtt
     6781 tctggcagga gcataccatc atgtgaaaag tattggcgac atctgtgctt tgatatgggg
     6841 ataagatttg ttaacatatc atataaaggt ttcattcctt caaaatcacc gcttagaagc
     6901 atattccagt atatatgtct tgtattttga atccaatatg ctcctcccca tcttctgtaa
     6961 tcccaggtta ttgacccatt atctgattca aaaggatatg ttacaaaaat ggacccatta
     7021 aactttatag gtacattacc ctttccagca catctgttaa aatacttctg atatatcca
//
LOCUS       DAOSXM010000101         7028 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2111
            NODE_6969_length_7028_cov_7.629571, whole genome shotgun sequence.
ACCESSION   DAOSXM010000101 DAOSXM010000000
VERSION     DAOSXM010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864178
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 7028)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7028)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:24:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,261
            CDSs (total)                      :: 2,229
            Genes (coding)                    :: 2,218
            CDSs (with protein)               :: 2,218
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 3 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7028
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6969_length_7028_cov_7.629571"
                     /isolate="AMR_MDS_2111"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..405
                     /locus_tag="PLQ68_09850"
     CDS             <1..405
                     /locus_tag="PLQ68_09850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005352568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HXK72998.1"
                     /translation="IYNKEYLTDCEFYVDVLIKNGVLDKDILREDKSQHTRDNAFLSR
                     EITDEAGLDIYKALIVCKSFHARRCLMLYQMAFPKTEIHVIPVDCYGITKDNWYTFEY
                     GIDRVLGELSRCGNQFVGDIKKYLREEKNCSD"
     gene            577..1152
                     /locus_tag="PLQ68_09855"
     CDS             577..1152
                     /locus_tag="PLQ68_09855"
                     /inference="COORDINATES: protein motif:HMM:NF016763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HXK72999.1"
                     /translation="MSKKQIVLNLILFLFTTATTILISGITIDRLIEPLRLGYFGVIC
                     YKIFLSYCLYLLIIWICINSISLFYKKIFLYLYIALITGFLLSRNSDVALINLQLGKI
                     DDYSVLTLFGNMLMFLTVSYLMKYLYKIKTMYTILFISISIIILEVLQYILRKGCLDI
                     NDIILNIFGAVLGVVLYNVADRNSKIKQEKR"
     gene            1327..3915
                     /locus_tag="PLQ68_09860"
     CDS             1327..3915
                     /locus_tag="PLQ68_09860"
                     /inference="COORDINATES: protein motif:HMM:NF024702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat protein"
                     /protein_id="HXK73000.1"
                     /translation="MKCTKRIRILLILLVILLVLSSCDKPIVTISPGNSEVIESVLPS
                     PIESPSASLEPSPSIEPTPSQTVQPSPSIEPTPTYTFTPPPTPTPTPVPQRPEAFTPE
                     QYFTFDAMHGIILDYDIEGGAYVNIPDKIGGVVVEQIGKHAFAYKGLYGVIIPDTVKV
                     VGESAFANNNNLMPFKELTLGKNVEVIKDNAFDACCMNSLILPDKVMYIGESAFSSYK
                     SDIVIPDSTEYIGVNAFASLSQSNITLGKNVTSFYVIEGDAIAGYICKPDNHLTIPTI
                     PGVDTIKDNAFTGYSIESIIIPESIRRIGNRSFFLAAKNITIPDSVISMGNNAIFEGA
                     IITCGSGSFAEYYARKIKYELSESFVPVDFPIDREGILSADISEISGKDYSYPFENWA
                     RLYLNSKYSTYEITRLKVNADINYTNYIDYDISNTLFCDDYTVAAKYYKVTLKTNKDI
                     PDLQRNSSGDYYFDFQVVALYNAEGKVVLSGFIENKENLLKTSYNLYSYIKEDTRFIY
                     NDFNAPPSILNSSTSTEIINLSDYSMVNDIVKDVDFAAVWYIEEYVPISKDVIAFFVP
                     SPSSTVYMVNLKTNEILFSQKYDYYGVSLSSEDGVCVILYYTQEVRIRSFDYLNDKGE
                     IIRQITYVDYPNDKKSIDIPGTPYELIQENSSISIQNISTGSVNILLQSKKVDFHKDL
                     FYYIDKVIDSSRFIYTCFEMQGRDPGWNRTYIYDIRDGSSLKIHSSEDNRYYEYAGYY
                     GGRHFIKFSNTFDEANKLYILETEGANAGSLECITDNIDNYLAIKHYSISSDGKYLFV
                     MTSNRLSDDMNRYNSSERIAIVDIQTKAVIYQDEYIQEYGSVTYLEDGYFCMNNGKTL
                     IYLNMK"
     gene            4011..4913
                     /locus_tag="PLQ68_09865"
     CDS             4011..4913
                     /locus_tag="PLQ68_09865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012199017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family protein"
                     /protein_id="HXK73001.1"
                     /translation="MKIDRLIGIISVLQNSKKVTAPYLADMFEVSRRTINRDIEDICK
                     AGIPLVTTQGKNGGITVMEGFNIERALLTEDELQSIFIGLKSIDSVSDVPVAKMLSLK
                     FRGSDLNEEINSNINIDLSSFYKESYTKKISVIRKAIRENKLISFHYYYKKGEADKFV
                     EPYMLMYKWSSWYVYGFCIERQDFRMYKLNRLWNLQITDNTFVPRKINSEDINPGSNI
                     TDECVIKVIFDQCVKYRLIEDNGPDSFTVMEDGKLLFEWKCTDADDALYQILAYGEHA
                     TIVDPPEMKIRIKEIIQEMLNKYD"
     gene            5007..5678
                     /locus_tag="PLQ68_09870"
     CDS             5007..5678
                     /locus_tag="PLQ68_09870"
                     /inference="COORDINATES: protein motif:HMM:NF020922.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2461 family protein"
                     /protein_id="HXK73002.1"
                     /translation="MQTADREFNGFPVEMTKYLYELKYTNTVEKQDENIIKYKEYITT
                     PLILLYESLVPTVKAISGNLEATPRRCISTPYTDRRFSPNAPLKEYMYIRYRHACREK
                     DNIGLYFDMGIEGYSFGMRIYDQTSEGMSKIRQMILNHPKKFEIQLGKLKEAGFLVFG
                     EEYKKDHYPDMDSYLLKEILNRKHFHICKDNTLNDNIYTSQLKEDIADGFNEIKGFIK
                     LLEEM"
     gene            5680..6327
                     /locus_tag="PLQ68_09875"
     CDS             5680..6327
                     /locus_tag="PLQ68_09875"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HXK73003.1"
                     /translation="MKIEMGNTRPEHYKEYWPGQYKFFHHFEFLCGIPSVMYAITTIK
                     ENGMPNVCLNAWSSFTNDNNSFYVVMTGISHHCHTYKNIARTGEFVINFINHEYFDNC
                     MKTIEDNKYDTDELQAGGFTKEDSVTVNCPRLKESFLSIECRLEKDIKLTDEGNASIL
                     VGKVMHIAADEDYAKGIDDKYSDKGFMFNIHAPKDYTSGEGDKSAVAVCKTVRVY"
     gene            6379..6747
                     /locus_tag="PLQ68_09880"
     CDS             6379..6747
                     /locus_tag="PLQ68_09880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXK73004.1"
                     /translation="MIIPHLHFTGTCKEAIELYEKAFKTKTDEIYMNEDNKVSHAVMS
                     IHNQTVYLNDRFGAKERTTDISTNLIIVFDNTEEFMYSYGILKEGSKEIDPISTRPYS
                     SLVVQFIDKYNVQWAFMVKI"
     gene            6827..>7028
                     /locus_tag="PLQ68_09885"
     CDS             6827..>7028
                     /locus_tag="PLQ68_09885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK73005.1"
                     /translation="MNINKETVTELIMERNFLTKKADTDEYLSLYHDTQPVQSKYWNG
                     FGQPPTITFRTDFDDIAFNKRRQ"
BASE COUNT         2566 a          999 c         1339 g         2124 t
ORIGIN      
        1 atatataaca aagaatattt gactgactgc gaattctatg tggatgtttt aataaaaaat
       61 ggtgttttgg ataaagatat attaagagaa gataaatcac agcatactcg tgataatgca
      121 ttcttatcaa gagagataac agatgaagcg ggactggata tatataaagc actgattgta
      181 tgcaaatctt ttcatgcaag aagatgtctg atgctttatc agatggcatt tcctaaaaca
      241 gaaattcatg taataccagt agattgttat ggaataacaa aagataactg gtataccttt
      301 gaatatggaa tagacagggt tttaggagag ctttcaagat gcggaaacca gtttgttggg
      361 gatataaaga agtatcttag agaagagaaa aattgttcag attaaggggt aagaacaaag
      421 aatattaaga agtaaagcag taaatttgag aaggttactt ttaactgttt tacgttatac
      481 atgatattaa agtaagaaaa atacagatac taataattat aatcagtata caagaacgta
      541 taaaagctaa ccttgtagaa ggagaaaatc tttgttatga gtaagaagca aatagtactg
      601 aatctgattt tattcctatt tacaacagct acaacaattt tgatatcagg tattactata
      661 gatagattaa tagagccgct tagattggga tattttgggg ttatatgcta taagatattt
      721 ctatcatatt gtttgtatct tttaattata tggatttgta taaattcaat ttctttattc
      781 tacaaaaaga ttttcctgta tttatacata gctttaataa cagggttttt gctatccaga
      841 aacagtgatg ttgcattaat aaacctacag cttgggaaaa ttgatgatta ctctgtgtta
      901 acattgttcg gaaacatgct tatgttcctg acggtcagtt atctaatgaa atatttatat
      961 aaaattaaaa ccatgtatac aatattattt atttccatct ctattatcat actggaagtt
     1021 cttcaatata tattgcgtaa aggctgttta gatataaacg acataatatt aaatattttt
     1081 ggggctgtac taggtgttgt tctgtataat gtggcagaca gaaatagtaa aataaaacaa
     1141 gaaaaaaggt aattaacctt tgtatgctat agctggattg ttataggaaa gttttgcaga
     1201 aatacatgta tggatagtta gattttgtct ggtatatacc tttattaatt ttaaaattta
     1261 tattaaaaca ggattgatga ttagttacca aaagtatata ctattggtaa ctaacaggag
     1321 aaaattatga aatgtactaa aaggattcgt atattactta tattattagt cattttactt
     1381 gttctatcat catgtgacaa gccaatagta acaataagcc ctggcaattc cgaggttata
     1441 gaatctgtat taccaagccc tattgaatcg ccttctgctt cattagagcc ttcacccagt
     1501 atagagccta caccaagcca gacagttcaa ccatcaccta gtatagaacc tacaccgacc
     1561 tacacattta ccccgcctcc aactcctaca ccgacacctg taccgcaaag gcctgaagct
     1621 tttacccctg aacagtattt cacttttgat gcgatgcatg ggattatatt ggattacgat
     1681 atagaaggtg gagcttatgt taacattcca gataaaatag gtggtgtagt agtcgagcag
     1741 ataggcaaac atgcatttgc atataaaggc ctttatggag taatcattcc tgatacagtg
     1801 aaagtagtag gtgaatccgc atttgcaaac aataacaatt taatgccatt taaagaatta
     1861 acccttggga aaaatgttga agttattaaa gacaatgctt tcgatgcctg ctgtatgaat
     1921 tcccttatac tgcctgataa agtaatgtat ataggagagt cagctttttc cagttataaa
     1981 tccgatatag ttatacccga tagtactgaa tatatagggg taaacgcttt tgcttcatta
     2041 agtcagagta atattacatt aggcaaaaat gtaacatcat tctatgttat tgaaggagat
     2101 gctattgccg gatatatatg taaaccggat aaccatttaa ctattccgac tattccggga
     2161 gttgatacta tcaaagataa cgcattcaca ggttattcca ttgagagtat cataatacct
     2221 gaaagcatca gaagaatagg gaaccgttcg ttttttttgg ctgccaaaaa tattacaata
     2281 ccggacagtg ttatatccat ggggaataat gcgatttttg aaggtgcgat tattacttgc
     2341 ggatcaggtt cgtttgcgga atattatgca aggaaaatta aatatgaatt atccgaaagc
     2401 tttgttccgg tggatttccc aatagacaga gaaggaatac tcagtgctga tatttcagaa
     2461 ataagcggaa aggattattc atatccgttt gaaaactggg caagactata tttaaacagt
     2521 aaatatagca cttatgaaat aactagactt aaagtaaatg ctgatattaa ttacactaac
     2581 tacattgact atgatataag taatacacta ttttgtgatg attacactgt cgctgcaaaa
     2641 tactataaag taactttaaa aactaacaag gatatacctg atttacaacg aaatagttca
     2701 ggtgattact atttcgattt tcaggttgtg gctctgtaca atgcagaagg taaggttgtg
     2761 ctttctggtt ttattgagaa taaagagaat ttgctcaaaa cttcctataa tctgtacagt
     2821 tatataaaag aagatactag atttatatat aacgatttta atgctccacc aagcattctg
     2881 aattcatcaa cctctacaga aataattaat ctttctgact atagcatggt taacgatatc
     2941 gttaaggatg tagattttgc tgcagtatgg tatattgagg agtatgttcc tatcagtaag
     3001 gatgttattg ccttttttgt tccaagcccc agctcaactg tatatatggt aaacctgaaa
     3061 accaatgaaa tcctattttc gcaaaaatac gattattacg gtgtcagttt atcatcggaa
     3121 gatggtgtat gtgtgatttt gtattataca caagaggtaa gaatccgtag ttttgactat
     3181 ctgaatgata aaggtgagat aattagacag attacatatg tggattatcc caatgataaa
     3241 aaatcaatag acataccggg tactccatat gaattaattc aggagaatag ttccattagc
     3301 atacaaaaca tatcgacagg ttctgtgaac atattgctac agagtaagaa agtagatttt
     3361 cataaggatc ttttttacta catagataaa gtaattgaca gtagcagatt catatacaca
     3421 tgttttgaaa tgcagggacg cgatcccggt tggaatcgca catatattta tgatatacgt
     3481 gatggaagca gtcttaagat acatagttct gaggacaata ggtattatga atatgcaggt
     3541 tattacggag gtagacattt cataaagttt tcaaacacat tcgatgaagc caacaagctg
     3601 tatattttgg aaactgaagg tgcgaatgcg ggaagtttgg aatgtataac cgataacatc
     3661 gataactatt tggcaattaa acattatagt ataagcagcg atggcaaata tttatttgtt
     3721 atgacatcaa acagactttc tgatgatatg aaccggtata actcaagcga acgtatagcg
     3781 attgttgata tacagacaaa agcggttata tatcaggatg aatatattca agagtatggc
     3841 agtgtaacct atttagaaga cggctatttc tgtatgaata atggaaagac attgatatat
     3901 ttgaatatga aatgatgtaa gatctgagtt taatagctga agaaaaagtc agatactttg
     3961 atgtactggc ttttccatat gtgataatat aatacgcaaa aaggtatgat atgaaaatag
     4021 acaggcttat cgggataata tcagtattgc agaatagtaa gaaggtaaca gcgccttatc
     4081 ttgctgatat gtttgaggta tcaagaagga cgattaaccg tgatattgaa gacatatgca
     4141 aagcagggat acctcttgtt acaactcagg gtaaaaatgg tgggattact gttatggagg
     4201 gttttaatat tgaaagagct cttcttactg aagatgagct tcagtcaata tttataggac
     4261 ttaagtccat tgacagcgta tcagatgttc ctgttgcaaa gatgctttca cttaaattca
     4321 gaggaagtga tttaaatgaa gaaattaaca gtaatattaa tatagacctt tcatctttct
     4381 ataaagaaag ttacacgaaa aagatttcag ttataagaaa agcaatcagg gaaaataaat
     4441 tgatttcttt tcactattac tataaaaaag gcgaagcgga taaatttgtc gagccatata
     4501 tgcttatgta taaatggtca tcctggtatg tatacgggtt ttgtattgaa agacaggatt
     4561 ttcgaatgta taagcttaac aggttatgga atctgcagat tacagataat acattcgtgc
     4621 caagaaaaat aaatagtgaa gatataaatc caggcagcaa tattacagat gaatgtgtaa
     4681 taaaagtaat ctttgaccaa tgcgtcaagt accgccttat agaagacaat ggtcctgatt
     4741 catttacagt aatggaagac ggaaagcttc tttttgaatg gaaatgcacc gatgcagatg
     4801 acgcgttata ccagatactt gcatatggtg agcatgctac aattgttgat ccgccagaaa
     4861 tgaaaatcag gataaaagaa ataatacagg aaatgcttaa taaatatgat tgaacatgac
     4921 atatagatgt catgttatat gtgctagaat gggtatcgat aaggatacct ttccttatac
     4981 acaaaaagca agctatggtg ggttaaatgc aaacagcaga cagggaattc aacggctttc
     5041 cagttgaaat gacaaaatac ctatatgaac tgaaatatac aaatactgtg gagaaacagg
     5101 atgaaaatat tattaagtac aaagagtaca taacaacccc ccttatactt ctctatgaat
     5161 cgcttgtgcc aacagtaaaa gcaataagcg ggaatttaga agcaacgccg agacgatgca
     5221 tatccacacc atataccgac agaaggtttt ctcccaatgc tccgttaaaa gaatatatgt
     5281 atatcagata ccgccatgca tgcagagaaa aggacaacat aggcttgtat tttgatatgg
     5341 gaatagaagg ctattcattt ggaatgagga tatatgacca gactagtgaa gggatgagta
     5401 agataagaca gatgatatta aatcatccta agaaatttga gatacagtta ggaaaattaa
     5461 aggaagccgg ttttcttgtg tttggggagg aatataaaaa ggatcattat ccagatatgg
     5521 attcatactt attaaaagag atactgaaca ggaaacattt ccatatatgc aaagacaata
     5581 cattaaatga taatatatat acatcacaat taaaagaaga catagcggac gggttcaatg
     5641 aaataaaagg atttataaaa ttacttgagg aaatgtaata tgaagataga aatgggtaat
     5701 acaagaccag aacactataa agaatactgg ccggggcagt ataagttttt tcatcacttt
     5761 gagtttttat gcggtatacc aagcgtaatg tatgcgatca ctacaattaa agaaaatgga
     5821 atgcctaatg tatgtttaaa tgcatggagt tcctttacaa atgataataa ctctttctat
     5881 gtcgttatga ctggtatatc gcaccactgt catacataca agaatatagc aaggacaggt
     5941 gaattcgtta ttaacttcat taaccatgaa tatttcgata actgtatgaa aacaatagag
     6001 gacaataaat atgatacaga tgagttgcag gcaggcggat ttacaaaaga agattcagtt
     6061 acagtaaatt gcccaaggtt aaaagaatcg tttttatcta tagagtgcag actggaaaaa
     6121 gatataaagc tgactgatga aggtaatgct tcaatacttg tcggtaaagt aatgcatata
     6181 gctgctgatg aagattatgc aaaaggtatt gatgataagt attctgataa aggctttatg
     6241 ttcaacatac atgctcctaa agactacaca tcaggagaag gggataaaag tgcagttgca
     6301 gtatgtaaga ctgttcgggt atactgaaaa ttgcataaac gaaccgtcta aatagagaat
     6361 aattcaagga gaacagtaat gataatacca catctgcatt ttacagggac ttgcaaagaa
     6421 gcaatagaac tttatgaaaa agcttttaag acaaaaacag atgaaatata catgaacgag
     6481 gacaataaag tatctcatgc tgtaatgagc attcacaatc agactgttta tcttaatgac
     6541 cgttttggag caaaggaaag aacaacagat atatcaacga atcttattat agtttttgat
     6601 aatactgaag aattcatgta cagctatggg atattgaaag aaggaagcaa ggaaattgat
     6661 ccaataagca ctcggccata cagctcttta gttgttcagt ttattgataa atacaatgtc
     6721 caatgggctt tcatggttaa gatatagcca tctggtacgc tatattgcat taaattactg
     6781 gagaatgaca atagatatta gaaagaggat aaatagaaga taaaagatga atataaataa
     6841 agagacagtt actgaactca taatggaaag aaatttcctt accaaaaaag cagatacgga
     6901 tgaatatctc tctctttatc atgataccca gccagtacag agtaaatact ggaacggttt
     6961 cggacagcct cctacaatta catttcgtac agactttgac gatattgcgt ttaataaaag
     7021 aagacaat
//
LOCUS       DAOSXM010000102         6819 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2111
            NODE_7259_length_6819_cov_10.668244, whole genome shotgun sequence.
ACCESSION   DAOSXM010000102 DAOSXM010000000
VERSION     DAOSXM010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864178
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 6819)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6819)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:24:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,261
            CDSs (total)                      :: 2,229
            Genes (coding)                    :: 2,218
            CDSs (with protein)               :: 2,218
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 3 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6819
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7259_length_6819_cov_10.668244"
                     /isolate="AMR_MDS_2111"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            112..582
                     /locus_tag="PLQ68_09890"
     CDS             112..582
                     /locus_tag="PLQ68_09890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK73006.1"
                     /translation="MKKQRIVKILTLALAIVLVLSLAGCGPKGRVKDAVEAFIEKANA
                     LDFQGMFDLMATETVREIRGNKSVEEFMADKTDEEIAAAITRQILPAQVDDPIGFLKT
                     LAMEYNGADIREDFSSLYATVTFTINGETTSKEAVFNIVRKNVSEPWTLRGIAL"
     gene            688..1224
                     /gene="thpR"
                     /locus_tag="PLQ68_09895"
     CDS             688..1224
                     /gene="thpR"
                     /locus_tag="PLQ68_09895"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HXK73007.1"
                     /translation="MRTFIAINFTQDIKNRLISVERDLKQMSQKGRFTSKDNLHLTLV
                     FLGEIAEDRAKLAQLCVKNITAPSFEMTVNRIGCFKRSDGDIYWAGIETNQILNSIYE
                     QLYKNLNSYDFYIRDTEYIPHLTLARDYILKDNADIEEYKKNFEKINIRVDNIQLMKS
                     EVIDNKLTYTALYTKALD"
     gene            complement(1345..2208)
                     /locus_tag="PLQ68_09900"
     CDS             complement(1345..2208)
                     /locus_tag="PLQ68_09900"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HXK73008.1"
                     /translation="MYRVGMLGTENSHALAFAKEICFKEEYKDFKITAFYALEKAPSE
                     AIIEKCGITDAVIVDDPMEMLDLVDCVIVTNRHGKYHESCALPFIERGMPAFIDKPFT
                     IDPAEARNLLRAAKQNKVPISGGSGCKYSPQILELKKLVKEDAFGKIYSGVMNFPAST
                     ESEYGGLYFYASHLVEMTLAVYGQDMLSVSAFINNGHITAIAHYDKYDITMQFAASKK
                     YASVIYAENDVDVRYLDMSSIFIVEFGHFVDMVRTGKEPLSHDELLMPVLVMNAIEKS
                     IETGHTVNIDR"
     gene            complement(2223..3470)
                     /locus_tag="PLQ68_09905"
     CDS             complement(2223..3470)
                     /locus_tag="PLQ68_09905"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009792862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HXK73009.1"
                     /translation="MKQELALYGGEKVKQTPYGTGKRFGDEELVQLKEALDQNTLFYW
                     SGNKVKTLCSKFAQMYGVENCVAASSGTAAIHVALGALGVTEGDEVITSPITDMGSII
                     GIMYQNAIPVFADLDPHTYNMDPKSIEKCITEKTKAIVVVHLAGNPAEMDEIMEIARK
                     HNVYVVEDCAQSYMCKYKGRLAGTIGDMGCFSLNDFKHISAGDGGMVLVNRPELYVKT
                     FMFADKNYNRFAKGPSALRMIEYLAPNYRMNELTAAVGIAQLDKLEKICSTRQKYGNM
                     ITNGIKHLEGVFPHEVKEGNESSFWFYMFRVDPKAINVDIKTYADALKAEGIGCQQGY
                     IPSVVYLYDMFQNKEGYAGTHAPFDSKYYGKDISYPKGLCPVSEEILDTAIRLNLNEF
                     YTEQDILDIIESIVKVTEYFKKG"
     gene            complement(3480..4262)
                     /locus_tag="PLQ68_09910"
     CDS             complement(3480..4262)
                     /locus_tag="PLQ68_09910"
                     /inference="COORDINATES: protein motif:HMM:NF016816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HXK73010.1"
                     /translation="MHIHIITDLEGISGVTTIEQMEFDSFGYEYARKRLMIDTNAAIR
                     GAFNNGADVVTVTDGHARGKNFIDELLDSRAKKINLIDLYSQGLHKEVDAVMFVGTHA
                     MPGTMNAFLDHVQSSTAWYNYYINNIKCGELVQGGAFLGFYGAPVIFASADEAACEEA
                     KYFFGNIQTAPVKKALTRNKAVSYDLDYCEKMIEDRASKAMNLIGSIKPYKINGPVEI
                     KVEFTRTDYCDNASERPNVERIDARTVIKKIPQITDYMSILL"
     gene            4620..5927
                     /locus_tag="PLQ68_09915"
                     /pseudo
     CDS             4620..5927
                     /locus_tag="PLQ68_09915"
                     /inference="COORDINATES: protein motif:HMM:NF024598.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycoside hydrolase"
     gene            6004..6477
                     /gene="smpB"
                     /locus_tag="PLQ68_09920"
     CDS             6004..6477
                     /gene="smpB"
                     /locus_tag="PLQ68_09920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HXK73011.1"
                     /translation="MEKEGIKVIANNRKAGFDYELIEFYEAGIVLSGTEVKSLRAGAV
                     NMRDSYGYIKDFEAFVSGMHISHYEKGNINNVDPDRDRKLLMHKSEIRKLVLKMQQKG
                     YTLVPTKIYFVKNKVKVELALAKGKKSYDKRDSIRKNEADREIQKRMKDSNHLGA"
     gene            6468..6819
                     /gene="ssrA"
                     /locus_tag="PLQ68_09925"
     tmRNA           6468..6819
                     /gene="ssrA"
                     /locus_tag="PLQ68_09925"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT         2253 a         1157 c         1242 g         2167 t
ORIGIN      
        1 gaaaaatata ttttttgaac taataatgtt gttataagcg gaaatatcat ataatattat
       61 aagagtgata aatacaatca taagttgaat tttaaataaa gggagcaaga aatgaaaaag
      121 caaagaatag taaaaatttt aacattggca ttagcaatag tcctcgttct gtctttagca
      181 ggctgtggac caaaaggaag agttaaagat gcagtggaag ctttcattga aaaagcaaat
      241 gcattggatt ttcagggaat gtttgattta atggcaacag agaccgtaag agaaatcagg
      301 ggaaataaat ctgttgagga gttcatggct gataaaacag atgaagagat agcagctgca
      361 ataacaagac agatactacc agctcaggtt gatgatccta tagggttttt gaagacattg
      421 gcgatggaat ataacggagc tgatataaga gaagattttt cctctttata tgctacagta
      481 acatttacaa ttaatggcga aacaacatca aaagaagcgg ttttcaatat tgtaagaaag
      541 aacgtttctg agccttggac attaagagga attgcactgt aattacaaaa aaagatatta
      601 aacaaaggaa gagcttgatg cttttccttt tttttattga aagaaaagta ataagtctat
      661 atacttaaag aaacaaggag acaagagatg aggacattta tagcaattaa ttttactcaa
      721 gatattaaaa accgccttat atccgttgaa agagacttaa agcagatgtc tcaaaagggc
      781 aggttcactt caaaggataa tcttcatctt acactggttt ttttaggcga gatagctgaa
      841 gacagagcaa agcttgcaca attgtgcgtt aagaatatta ctgcaccatc atttgaaatg
      901 acagtaaaca ggataggatg ttttaaaagg tctgatgggg atatctactg ggcaggtatt
      961 gaaaccaacc agatattaaa ttctatttat gaacaattgt ataaaaactt aaactcatat
     1021 gatttctata taagagatac tgaatacata cctcatctga cgctggcaag agattatatc
     1081 ttgaaagata atgcagatat tgaagagtat aaaaagaatt ttgaaaagat aaatataaga
     1141 gtagataata ttcagcttat gaaatcagaa gttattgata ataagcttac atatacagca
     1201 ttgtatacta aggctctaga ttagatatta attcatttaa cgactgcata ttttttgcta
     1261 ctaaaaactt aaactataca aaaaaaagca tcttgcgatg cctttttagt ttatgcctta
     1321 aattattatc aaacctgttt ctgtttatct gtcaatgttt acagtatgac ctgtttcaat
     1381 tgacttttca attgcattca tgacaagtac cggcattaat aattcatcat gggataaagg
     1441 ttcttttcct gttcttacca tatcaacaaa atgaccgaat tccacgatga atatgctgga
     1501 catatccaga taacgtacat caacatcatt ttctgcatat attactgatg catatttttt
     1561 tgatgctgca aactgcatag ttatatcata cttatcataa tgggcaatag ctgttatgtg
     1621 accattattt ataaatgctg atacagataa catgtcctga ccataaactg ccaatgtcat
     1681 ttcaacaaga tgagaagcgt agaaatatag tccgccatat tcgctttcag ttgaagcagg
     1741 gaagttcata acacctgaat agatttttcc gaatgcatct tctttgacta attttttcaa
     1801 ttcaagtatc tgtggggaat atttacatcc tgaaccgcct gatatgggaa ctttgttttg
     1861 ttttgctgct cttaacagat ttcttgcttc agcagggtct atagtaaaag gtttgtctat
     1921 aaatgcaggc atacctcttt caataaaagg caatgcgcat gattcatgat actttccatg
     1981 tctgttggtt actataacgc aatcaaccaa atccagcatt tccatcggat catcaactat
     2041 tactgcatct gtaattccgc atttttcaat aattgcttct gatggggctt tttccaaagc
     2101 ataaaaagca gttatcttaa aatccttgta ttcttcttta aagcatattt ccttagcaaa
     2161 agctaaagca tgtgagtttt ctgttcctag cattcctact ctgtacatat cattcctcct
     2221 ttttaacctt ttttaaagta ttctgttacc ttaactattg attctataat atccaatatg
     2281 tcctgttcag tatagaactc attaagatta agtcttattg ctgtgtccag tatttcttct
     2341 gaaaccgggc acaaaccctt aggatatgat atatcctttc cataatactt tgaatcaaaa
     2401 ggtgcatgag ttcctgcata accttcttta ttctggaaca tatcataaag atatactact
     2461 gatggtatat aaccttgctg gcagccgatg ccttcagctt ttaatgcgtc tgcatatgtt
     2521 ttaatatcca catttatagc ttttgggtca actctgaaca tatagaacca gaaactggat
     2581 tcattaccct ctttaacttc atgtgggaaa acgccttcta agtgcttaat gccattggtt
     2641 atcatgtttc catatttctg ccttgttgaa catatctttt ccagcttatc aagttgtgca
     2701 atgccgactg ctgctgtaag ctcgttcatt ctgtaatttg gtgcaaggta ttctatcatc
     2761 cttaatgcgc tcgggccttt tgcaaacctg ttgtaattct tgtctgcgaa catgaatgtt
     2821 tttacataaa gttcaggacg gttcaccaaa accattccac cgtcaccagc agaaatgtgt
     2881 ttgaagtcat ttaggctgaa acatcccata tctccaattg ttcctgctaa gcgtcctttg
     2941 tatttacaca tatagctttg agcacagtct tcaacgacgt atacattatg ttttcttgca
     3001 atttccatta tctcatccat ttcagctgga ttgcctgcaa gatgcacaac aactatggct
     3061 tttgtttttt cagttataca tttttcaatg cttttcgggt ccatattgta tgtgtgagga
     3121 tctaagtcag caaaaacagg aatggcgttc tggtacataa tccctattat acttcccata
     3181 tcagttatgg gggaggtgat gacttcatcg ccttcggtta ctcccaaagc tcctaatgct
     3241 acatgaatag ctgctgttcc tgatgatgct gctacgcagt tttctactcc atacatttga
     3301 gcaaatttag aacagagtgt tttaactttg tttccagacc aatagaacaa tgtgttctgg
     3361 tctagagctt ctttaagctg tacaagttcc tcatctccga atctttttcc tgttccgtaa
     3421 ggtgtctgtt tgactttttc tccgccatat aaggctagtt cttgtttcat gcgttcctcc
     3481 tataataata tcgacatata atcggttatc tgtggtattt tttttataac tgttctcgca
     3541 tctattcttt caacatttgg tctttcactt gcattatcgc agtaatctgt tcttgtgaat
     3601 tcaaccttta tttcaacagg tccgtttatt ttgtaaggct ttatgcttcc tatcaggttc
     3661 atggcttttg aagctctgtc ttctatcatt ttctcgcagt aatctaaatc ataagaaacc
     3721 gctttattcc ttgtcaatgc ttttttgaca ggtgcagtct gaatattccc aaagaaatat
     3781 ttagcttctt cacacgcagc ttcatcagct gaagcgaaaa taacaggtgc gccatagaat
     3841 cctaaaaaag cgcctccttg tacgagttcg ccgcatttta tgttatttat atagtaattg
     3901 taccaggctg ttgatgattg gacatggtct aaaaatgcat tcatagttcc tggcattgca
     3961 tgtgtgccaa caaacataac tgcatcaact tctttatgta atccttgaga atataggtct
     4021 atgagattta tcttttttgc tcttgagtca agtagctcat cgataaaatt tttacctctt
     4081 gcgtgaccgt cagtaactgt tacgacatca gccccattat taaatgcacc gcgtatagcc
     4141 gcattggtgt caatcataag cctttttctt gcatattcat aaccaaagct gtcaaactcc
     4201 atctgttcaa tagtagtaac tccgcttatt ccttcaagat ctgtaatgat atgtatatgc
     4261 ataaaatacc tcttgtccta tgttattcat tataccatag ataaatataa atcaagaaca
     4321 gaatggtatt ttaagctata atatatacat gagtgaaaaa ggatagataa atgcttaata
     4381 ataaaaaaaa gacttataac acaatatttt caattatcac agcattatta atattatgct
     4441 gctttattag tagtaatata ttaaaagtaa aaggttacaa tgaatatgtt aattcagata
     4501 atgtaaaagc tctttatgta agagcagata cggctgcttt gcgcttagac gataagtatg
     4561 ttcagattta caaacagtat atagaatatt taaaagaaga agagtatgaa aaagctaata
     4621 tgctaataac agatagcgga catatttcag cttttgaaaa gattataggc ctgatacttg
     4681 caacagaact taacggagta gttataaatg taaaagatga tgcaggaaat gttaccatcc
     4741 cttcacagag tgagtatata aaagagatag aaagctataa tggacagctt tatgatatag
     4801 atacaaaaat cgaaaaattg aagaattatg gtatatacac aatagccaga gttgtagtct
     4861 ttaaagatga tatcctttct gaaaagaagg agcattccat taaatatgag aatggtgaat
     4921 tgtggaggga ttattccaat tttgcatggg taaacccgta tgatgagtat gtgcagcagt
     4981 acaatattga agtagcaaag caggctgcgg ttactggatt tgatgaaatt caatttgatt
     5041 atgtaaggtt tcctgagaaa ttctctcagt atgagatttc caaaagatat ttagatgaaa
     5101 atataaggca agattagtta atacggctat ttttaaatac agcgtacagt cagttaaatc
     5161 catataatgt gaaaatatca gctgatgtat tcggatgtgt agcacattta tgggatgacc
     5221 ctttaaatat tgatataggt caaatctggt ataatttaac tcaggaagtg gactatataa
     5281 gtcctatggt atatccgagc cactacagag gaactaactg gtatacatat aatgatccaa
     5341 ataagcatcc ttatgaagtt gtaaaaggag ccattgatga ttctttatac atcaattccg
     5401 cattcaaaga cagagcaaaa atccgattct ggcttcagga tttttccatg tatggatatg
     5461 agtatgggcc gatgcagatt cttgaccagg tcaaagcact acatgaaaag ggcattgata
     5521 cgtatatgtt ctggaacaac aagaacatat atgaacctga taactattta atattagaat
     5581 cacgaactgt agcagacata tcaaacagat accatgtaca tcagattagc aggaataacc
     5641 caattgatgc agtaaaaagg tatattgatg ctaatatctc aaaagatcca tatgaatcat
     5701 ttgtactgac agctataaac aacagagatg gagagtacaa ggattttata gttaatatta
     5761 aatatcttga cattaaaagc tatgagataa ctgattccaa gtccagtttt aatactgccc
     5821 aggtattttt aaatgtcaaa acagttactg aaactgaaaa gtgggttgtc tttctgattc
     5881 ttgaacaagg catatggaaa ataaacggtt attacgttat ttactgattt attaaatatt
     5941 tgtaacaatt aacaaataat tcacatcttt atggtataat tggtctccga aggagatgat
     6001 ggcttggaaa aagaaggaat taaggtcatt gcgaataata gaaaagccgg ttttgactac
     6061 gaactgatag agttttatga agccggaata gtgttaagcg ggaccgaagt gaaatctttg
     6121 agagccggag ctgttaatat gcgcgattct tacggatata ttaaagactt tgaagcattt
     6181 gtatccggaa tgcacatctc ccactacgag aagggaaaca ttaacaatgt tgacccggac
     6241 cgtgacagga aacttcttat gcacaagagc gagattagga aactcgtcct aaaaatgcag
     6301 cagaagggat ataccctggt tcctacaaaa atttattttg taaagaacaa ggtaaaagta
     6361 gagttggctt tagccaaagg aaagaagagc tatgataaac gcgattcaat ccggaagaat
     6421 gaagctgaca gggaaataca gaagagaatg aaagacagta atcatttggg ggcgtaaagg
     6481 cttcgacggg atgaatgaat tattgatagc gtgcagagga tcctcgtggg cctctaaaaa
     6541 aaacgaggga ataaatataa acgcaaaaac aaattttgct cctttagcag cgtaagctag
     6601 ctaaacatcc taccggaaat cacggggtcc ggaccagggt gtaatctacc gtgaaaaccc
     6661 gatagtcaaa gctttgagac taaagggtaa ctatgaagct accaaaatcg gacccggctt
     6721 acaggggact ttagagggaa attaaacagt aagctacgca cgtagaagtc ggtatggatt
     6781 ttgtttcgga cgagggttcg attccctccg cctccacca
//
LOCUS       DAOSXM010000103         6807 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2111
            NODE_7279_length_6807_cov_11.244076, whole genome shotgun sequence.
ACCESSION   DAOSXM010000103 DAOSXM010000000
VERSION     DAOSXM010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864178
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 6807)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6807)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:24:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,261
            CDSs (total)                      :: 2,229
            Genes (coding)                    :: 2,218
            CDSs (with protein)               :: 2,218
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 3 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6807
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7279_length_6807_cov_11.244076"
                     /isolate="AMR_MDS_2111"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            85..576
                     /locus_tag="PLQ68_09930"
     CDS             85..576
                     /locus_tag="PLQ68_09930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HXK73012.1"
                     /translation="MNTLGRKIAELRRGKNITQDEMAEKLNVTPQAVSKWENDISCPD
                     IMLLPKIAEMLSVSVDELLSDAPKKNVEFVPEGKRKNIDDLMFRIYVNSADGDKVKVN
                     LPMPLVKMGLEMGLKMPAVTGNEALKGIDFAQLLTLVDQGVLGRLVEIESADGDTVEI
                     VVE"
     gene            578..838
                     /locus_tag="PLQ68_09935"
     CDS             578..838
                     /locus_tag="PLQ68_09935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK73013.1"
                     /translation="MHIKIKAKDENKHINILLPTSLVCSNLTAIIAHKAITSKVEKEK
                     FSISRKDLARIMWMLRSLKKKYGKLELVDIESADGDIVKISL"
     gene            complement(897..3518)
                     /locus_tag="PLQ68_09940"
     CDS             complement(897..3518)
                     /locus_tag="PLQ68_09940"
                     /inference="COORDINATES: protein motif:HMM:NF036578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HXK73014.1"
                     /translation="MRIYDITINDLFEPILDSVPRISWKTEAENINDIQKSYQVIVFK
                     TEDNEIVWKSDVVFSKNSRYIPIKADLKPLTEYTCELLIQNQSDILFKSAPFTFLTGK
                     LNSIWSGKWIRAYYAKEKGEVNHCASYLRKEFIIDKKVSKALLVICGLGQFEASINNK
                     KVGTDFLSTPYTDFSKQVQYRIYDVKKYLENDENAIGVILGNGFYNSTTNDPWQCSSA
                     IWKDCPKLIAELHITYFDGTSQVLNSDDTWQSSKGPIIFNGMRHGEIYDARLEMDGWN
                     MPRFQSDLWKNVKYVRAPGGILHAMEMEPICVRHVYKPISKWKSNDGWLFDIGQSQSG
                     IAHFALKGPKDKKITIRYSDILHEDNSLNQEALSSFNQDAPFHTDIYIKRSDDVEYYH
                     PIFVYHGFRYLHIECDYEPNLDDVEVWSLCNDIKEKSQFECSSDTINAIQNLCIKSSE
                     SMCFSLMASDTAREQTSWTGDTALSVEQMATNFHCEAFFRNWQQVLRDAQLKSGTIPC
                     IVPSAGWGYNGMNGPDWSHSIYEVPLQFYRYSGDVEMIKDNYTALSKYCDYLDVMANE
                     GIVNYGLGDWCPPFLGKAISVNMESFKCPIEVSDTAYYHSTLKAAQTSAEILSMHNEA
                     EDFKKRAEYVKQQFRKNFFDKSKYSVKGNCQTATAMMIFHNLANEDEVPFLAAKLVQQ
                     IKDNDYALDYGILGNKAVINALGENGYADLVITMLTRDSFPSMKNWLDMGATTLWECW
                     NGGGSRNHHMFSCVSEFFYKYIAGIQPHVSAKGYDNILFNPPFDIQINNARATVNTPN
                     GKACIDWKKNNFEYQLQLTVPDNTNAILNLPCIFKGQDIKSEIVRNNLKPELKNGTRY
                     SVNLSAGKYSVNILIKD"
     gene            complement(3536..4069)
                     /locus_tag="PLQ68_09945"
     CDS             complement(3536..4069)
                     /locus_tag="PLQ68_09945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012803180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HXK73015.1"
                     /translation="MSSVNILLAGFGGQGILFLGKMTAYAGLADDKQITWLPSYGPEM
                     RGGTANCSVCISDELIGSPLVTNPDILVAMNLPSLEKFIDSVTAGGYVFLDSSMIDKT
                     IQRDDIHVYYIRATKIAEENNLTGLANMIMLGKMLKETGFCTECAIDQALLKCVPANK
                     KDMIELNKKAIKIGTDL"
     gene            complement(4066..4815)
                     /locus_tag="PLQ68_09950"
     CDS             complement(4066..4815)
                     /locus_tag="PLQ68_09950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013297033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HXK73016.1"
                     /translation="MAIVFQKPKALLDVPFTYCPGCTHGIIHRLVAETIDELGIEGNT
                     VGVAPVGCAVMNYNFFACDMVQAPHGRAPAVATGLKRAIPENVVFTYQGDGDLAAIGT
                     AETVHAAARGENITIIFVNNAIYGMTGGQMAPTSLPNQVTQTTPYGRDTSYSGYPIRV
                     CEMLATLDGTALAQRVTVDNVKNVLLAKKAIKKAFTYQIEKKGFSIVEVLSTCPTNWG
                     LNPEESLKWLRDNMVPYYPLGVYKDVMEVSK"
     gene            complement(4815..5876)
                     /locus_tag="PLQ68_09955"
     CDS             complement(4815..5876)
                     /locus_tag="PLQ68_09955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013979750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HXK73017.1"
                     /translation="MMAKVLMKGNEALAEAAIMAGCKHFFGYPITPQTELAAYMAKKM
                     PKIGGVYLQAESEIAAINMVLGASAAGKRAMTSSSSPGISLKAEGISYIAGSDLPAVI
                     VNVQRGGPGLGGIQPSQADYWQATKATGHGDFHLMVYAPSTVQEMMDLVRLAFDNADK
                     YRMPVMILSDGMLGQMMEPVEIKKEEAAEPIDKPWAACGHKNKRAHNVVNSLYLTPQA
                     LESLIKERYLKYDQIKKTEQMADEYMTDDADIIIVAYGASSRISRTAVNLARKEGIRA
                     GLIRPVTLWPYPDSFIEKHISHTKDFLCVEMSMGQMVDDVKLAVNGRKPVHFYGRTGG
                     IIPSPDEVLQELRKIGGKI"
     gene            complement(5880..6086)
                     /locus_tag="PLQ68_09960"
     CDS             complement(5880..6086)
                     /locus_tag="PLQ68_09960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015760731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HXK73018.1"
                     /translation="MAKAIINETLCKGCGMCIIACPKKILVLSPDKINSKGYHPAELI
                     DESRCTGCAACAIMCPDVAITVER"
     gene            complement(6101..6787)
                     /locus_tag="PLQ68_09965"
     CDS             complement(6101..6787)
                     /locus_tag="PLQ68_09965"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HXK73019.1"
                     /translation="MKKLKKIIIICGHYGSGKTNFSVNLALDLKKYNNDVTLIDMDIV
                     NPYFRSSDHKDLLESAGVKVISPVYANTNLDAPAIPAQMYSAFDTEGYAIFDMGGDDA
                     GATVMGQFFKNVTDKPYDMLYVINKNRSMIENAEDAANMLYSIQTASRLKATYIVNNS
                     HIKDYTTIQSVEESADYAKKISKLLNLEIYCTTISKKLLNEKGLRLSDEFVNNLYPID
                     IYVKTPWEEI"
BASE COUNT         1970 a         1320 c         1219 g         2298 t
ORIGIN      
        1 atggcacaga aactttggta gtttaggtat aagaatattg aattaaagga ataaaaaata
       61 tatattaaat agggagattg caaaatgaat acattgggaa gaaaaatagc tgaattaaga
      121 aggggtaaga atattaccca ggatgagatg gcagaaaagt taaatgtaac gccacaggct
      181 gtatcaaagt gggaaaacga tatatcctgt ccggatatta tgctgctgcc gaagatagct
      241 gagatgcttt cagtatctgt agatgagtta ttatccgatg cgcctaagaa gaatgttgaa
      301 tttgtacctg agggaaaaag aaagaatatt gatgacctta tgttcaggat atatgtaaac
      361 tctgctgatg gagataaggt aaaagtaaat cttccaatgc cattagtaaa gatgggactt
      421 gagatgggtc ttaaaatgcc agcagttaca ggtaatgaag cactcaaagg tatagatttc
      481 gcacagcttc taactcttgt tgaccagggt gttttaggaa ggcttgtaga aattgaaagc
      541 gctgatggtg atacggttga gatagtggtg gaatgatatg cacattaaga ttaaagcaaa
      601 agacgaaaac aagcatataa atatattgct tccaacaagt cttgtttgta gcaatcttac
      661 agccataatt gctcataaag ctattacaag caaggtagaa aaagagaagt tcagtataag
      721 cagaaaggac ttggcccgta taatgtggat gttaagatca ttaaagaaaa aatatggaaa
      781 acttgaactg gtggatatag aaagtgcgga tggagatata gttaagataa gtttatgatt
      841 acctcatcat aatatttgat atagcaaacc cggctgttat gccgggtttt tgctttttaa
      901 tctttaatta aaatattaac tgagtacttt ccagcagata agtttacaga atatcttgtt
      961 ccatttttta gttcaggttt tagattgttt cttacaattt ctgatttgat atcctgtcct
     1021 ttaaatatac aaggcagatt aagaatagca tttgtattat ctggaacagt taactgtaac
     1081 tgatattcaa aattattctt cttccagtca atacatgcct ttccgtttgg ggtatttaca
     1141 gttgctcttg cattgttaat ctgtatatcg aaaggaggat tgaaaagtat gttgtcatat
     1201 cctttagctg aaacatgagg ttgaattcct gctatgtatt tgtagaagaa ctctgacacg
     1261 cagctgaaca tatgatgatt tcttgaaccg cctccattcc agcattccca caaagtggta
     1321 gcacccatat caagccagtt tttcatgctt gggaaactat ctcttgtgag cattgttatt
     1381 accagatcag catatccgtt ttcgcctaat gcgtttatta cagctttgtt tccaagtatt
     1441 ccgtaatcca gagcataatc gttatcttta atctgctgta ccagtttagc tgctaaaaag
     1501 ggaacttcat cttcgttggc aagattatga aatatcatca ttgcagtagc tgtctggcag
     1561 ttgcccttta ctgaatattt tgatttatca aagaagtttt ttctaaactg ctgctttaca
     1621 tattctgctc tttttttaaa atcttctgct tcattatgca tactcaatat ttccgcactt
     1681 gtttgtgcgg cttttagtgt tgaatgataa tatgctgtgt cagacacctc aatcggacac
     1741 ttgaaagatt ccatgttaac cgaaatagct tttcctaaga aaggaggaca ccagtctccc
     1801 agtccgtaat taacaatccc ttcattggcc atcacatcca gataatcgca gtattttgat
     1861 aaagcagtgt aattatcttt tatcatttca acatcaccgc tgtaacgata gaactgtaga
     1921 ggcacttcat aaatggaatg actccagtca ggtccattca taccgttgta tccccaaccg
     1981 gcagaaggta ctatgcaggg gattgttcct gattttaact gggcatccct taaaacctgc
     2041 tgccagttgc gaaagaaagc ttcacaatga aaatttgttg ccatctgctc aacagataaa
     2101 gcagtatctc cagtccatga tgtctgctct cttgcagtat cggaagccat aaggctgaaa
     2161 cacatggact cagaagactt aatgcaaaga ttctgtatag catttatggt atcagatgaa
     2221 cattcaaatt gtgacttttc ctttatatca ttacataaag accagacttc tacgtcatca
     2281 agatttggct cataatcaca ttctatatgc agatatcgaa agccatgata tacaaatatt
     2341 ggatgataat attcaacatc atctgacctt tttatatata tatctgtatg aaaaggtgcg
     2401 tcctgattga aactggataa ggcttcctga tttaaagagt tgtcttcatg cagtatatcg
     2461 gaatacctga tggttatttt tttatccttt ggacctttca aagcaaaatg tgctatgccg
     2521 gactgagatt gtcctatatc aaaaagccat ccatcattgg atttccattt gcttataggt
     2581 ttgtagacat gtcttacaca tataggttcc atctccatag catgcaaaat tcctcctgga
     2641 gcgcgtacgt attttacgtt tttccataag tcagattgaa atctgggcat attccagcca
     2701 tccatttcca gtcgggcgtc ataaatttct ccatgtcgca taccgttaaa gataataggt
     2761 ccttttgaac tttgccatgt atcgtcactg ttaagaactt gtgatgttcc gtcgaaatag
     2821 gtgatgtgta attcagctat aagttttgga caatctttcc aaatagcgct ggagcactgc
     2881 caggggtcat ttgtcgtaga gttatagaaa ccattgccta gaatgactcc tattgcattc
     2941 tcgtcatttt ctaaatattt ttttacatca tatatcctgt actggacctg cttgctaaaa
     3001 tcggtatatg gtgtgcttaa aaagtccgtt ccaactttct tattgttaat agaagcttca
     3061 aactggccaa gaccgcaaat aacaagcagt gctttggata ctttcttatc aataataaat
     3121 tcttttctta aatatgacgc gcagtggttt acttcacctt tttcctttgc gtaataagca
     3181 cgaatccatt taccgctcca aattgaattt agcttgccag ttagaaatgt aaaaggagct
     3241 gatttaaaaa gaatatctga ttggttttga attaatagtt cacaagtata ttctgtcaaa
     3301 ggcttaagat cagctttaat aggtatatat ctgctatttt tcgaaaaaac cacatcactt
     3361 ttccatacta tttcattatc ttcagttttg aaaactataa cctgatatga tttttgtatg
     3421 tcattaatgt tttcagcttc agttttccat gaaattcttg gaacagaatc cagtatgggt
     3481 tcaaaaagat catttatggt aatatcatat attcgcatga agcctccttt tggtattaaa
     3541 ggtcagttcc gattttaata gctttcttat tcaattctat catgtctttt ttgtttgcag
     3601 gaacgcattt caataaagcc tggtctatcg cgcattcagt gcagaaacct gtttctttta
     3661 acatttttcc aagcattatc atgttagcca gtcctgttaa gttattttcc tctgcaatct
     3721 ttgtagcacg aatatagtaa acatgaatat catctctttg aattgttttg tctatcatgg
     3781 atgaatctaa aaatacataa cctcctgctg taactgagtc tatgaatttt tccagtgaag
     3841 gaagattcat ggcaacaaga atatccggat tagtaacaag gggagaacct atgagctcat
     3901 cagatataca tactgaacag tttgctgtcc cgcctctcat ttctggtcca tatgatggaa
     3961 gccatgttat ctgcttatca tctgctaatc cagcataagc ggtcattttt cccaggaaaa
     4021 gaatgccctg gcctccaaat cctgctaaaa gtatattaac tgaactcatt tggatacctc
     4081 cattacatct ttatacactc ctaatggata atatggaacc atgttgtctc ttaaccattt
     4141 caaagattct tcagggttta gcccccagtt tgtcgggcag gttgaaagca cttctactat
     4201 tgagaaacct ttcttctcaa tctgataagt aaaagctttc tttatggctt ttttggcaag
     4261 aagtacattt tttacattat caacagtaac tctttgtgca agtgcagtgc catctaatgt
     4321 ggcaagcatt tcacataccc ttataggata acctgaatat gatgtatctc ttccgtatgg
     4381 agtggtctgt gttacctggt ttggtaaact tgtaggtgcc atctgaccgc cagtcattcc
     4441 atatattgca ttatttacga atattattgt tatgttttct cctctagctg cagcatgaac
     4501 agtctctgca gtaccgatag cagcaaggtc accgtctcct tgataggtaa ataccacatt
     4561 ttctggaatt gctcttttaa gtcctgttgc aacagctggt gctctgccat gcggcgcctg
     4621 caccatatcg catgcaaaga aattatagtt cataacagca cagcctacag gagccactcc
     4681 cactgtattt ccttcaatac ccagttcgtc aatagtttct gctacaagcc tgtgtattat
     4741 cccatgagta caaccgggac agtatgtaaa aggaacatct aataaagctt taggtttttg
     4801 aaatactata gccattatat cttacctcca atttttctaa gttcttgtaa tacttcatcg
     4861 ggtgaaggta ttattcctcc tgtccttcca tagaagtgga caggttttct tccgtttaca
     4921 gcaagcttta catcatctac catctgaccc atactcattt caacacacaa gaaatcctta
     4981 gtatgagaaa tatgcttttc tataaatgaa tcaggatacg gccataaggt tacaggtctt
     5041 ataaggcctg ctctgattcc ttcttttctt gcaagattaa cagcagttct ggatattctt
     5101 gaagatgctc catatgctac gattataata tctgcatcat ctgtcatata ctcatcagcc
     5161 atctgctctg tctttttaat ctggtcatac ttaagatatc tttcttttat caaagattcc
     5221 aaagcctgag gtgtaagata cagcgaattt acaacattat gcgctctttt gttcttatgt
     5281 ccgcatgctg cccacggttt atctattggt tcagctgctt cctctttttt aatttcaaca
     5341 ggctccatca tctgtcctaa catcccgtca gacagtatca ttacaggcat tctgtatttg
     5401 tcagcattat caaatgccag tctgacaaga tccatcattt cctgcaccgt ggaaggagca
     5461 tataccataa gatggaaatc tccatgaccg gtagcttttg ttgcctgcca gtagtcagcc
     5521 tgtgaaggct gtatgccgcc aaggccggga ccgcctcttt gaacatttac tattacagcg
     5581 ggaagatcac ttcctgctat atatgatatt ccttctgctt ttaagcttat acctggagaa
     5641 gaagaggaag tcattgctct tttgcctgca gcagatgctc ccaataccat atttatagct
     5701 gctatttctg attcagcctg aagataaact cctccgattt taggcatttt ctttgccatg
     5761 tatgctgcca attctgtttg tggtgttata ggataaccga agaaatgctt acagcctgcc
     5821 atgatagcgg cttctgctag tgcttcgttt cctttcatta atacttttgc catcatatcc
     5881 tacctttcta ccgttatagc tacatccgga cacattatcg cgcatgctgc gcagcctgtg
     5941 catctggatt catcgataag ttcagcaggg tgatatccct tgctgttgat tttgtcgggt
     6001 gacagtacaa gtattttttt cggacatgct ataatgcaca taccgcaacc cttgcacaaa
     6061 gtctcattta ttattgcttt tgccatatat tcaagcctcc ttaaatttct tcccagggcg
     6121 tttttacata aatatctatc ggatatagat tatttacaaa ttcatcggat aatcgtaaac
     6181 ctttttcatt caagagtttt tttgatatag tggtacagta aatttccaaa tttaaaagtt
     6241 ttgatatttt tttagcataa tctgctgatt cttcaacaga ttgtatagtc gtatagtctt
     6301 ttatatgtga attattaact atatatgttg ctttaagcct tgatgcagtt tgtatggaat
     6361 aaagcatatt ggctgcatct tccgcgtttt ctatcatgga acggttcttg ttaattacat
     6421 aaagcatatc atatggctta tcagttacat ttttaaagaa ctgccccatt acagttgccc
     6481 ctgcatcatc acctcccata tcgaatatgg catagccttc agtgtcaaaa gcagaataca
     6541 tctgagcagg gattgcagga gcatccagat tggtgtttgc ataaaccggg gatatgactt
     6601 taactcctgc agattctaac aaatctttgt ggtcagaact tctaaaatac ggattaacta
     6661 tgtccatatc aataagtgtt acatcattat tatatttttt taagtcaaga gccagattga
     6721 cggagaagtt tgtctttccg ctaccgtagt gtccgcatat gattatgatt ttttttaact
     6781 ttttcatttt tcctgctaat aaaaatt
//
LOCUS       DAOSXM010000104         6791 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2111
            NODE_7309_length_6791_cov_8.067251, whole genome shotgun sequence.
ACCESSION   DAOSXM010000104 DAOSXM010000000
VERSION     DAOSXM010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864178
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 6791)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6791)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:24:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,261
            CDSs (total)                      :: 2,229
            Genes (coding)                    :: 2,218
            CDSs (with protein)               :: 2,218
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 3 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6791
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7309_length_6791_cov_8.067251"
                     /isolate="AMR_MDS_2111"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..354
                     /locus_tag="PLQ68_09970"
     CDS             <1..354
                     /locus_tag="PLQ68_09970"
                     /inference="COORDINATES: protein motif:HMM:NF014841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HXK73020.1"
                     /translation="VVSVHCPLNEQTLGMFNQDAFNKMKKNAMFINTARGAIVNEKSL
                     RNALENNTIASAAIDVLETEPMDKDCILYNVKNLIITPHTAWIPYETRKRLLGIVVSN
                     LKNFIDGNPTNVVSK"
     gene            complement(1306..2046)
                     /locus_tag="PLQ68_09975"
     CDS             complement(1306..2046)
                     /locus_tag="PLQ68_09975"
                     /inference="COORDINATES: protein motif:HMM:NF019322.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HXK73021.1"
                     /translation="MINKYSSTPLYYQLKEELIKSIIEGKYAPGEVIPSENELADLYS
                     LSRPTVRQAIGELAKIGYLIKIKGKGTFVADFVNKTTYDHTKGFIHCILDTINTSNRV
                     IIESTTINGSLELNRKFSTDYKSNYHEDYTKVTYTTETDNNIAYSISILPLNYFPNAA
                     ELLFKNVNSVDLLVGKYPLDPTNAKSSIKLVNADSDMCKIIKVPENAPLFKIETLLHG
                     RTGNVVEYNTTYYSAYNSVIEFNRSRRV"
     gene            complement(2083..3507)
                     /gene="purB"
                     /locus_tag="PLQ68_09980"
     CDS             complement(2083..3507)
                     /gene="purB"
                     /locus_tag="PLQ68_09980"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010076982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HXK73022.1"
                     /translation="MSDNYTSPFSQRYSSKEMQLIFSDDYKFSTWRKLWISLAKAQMK
                     LGLPITKKQIDELEENKDNIDYDQVKLFEKQIRHDVMSHIKAYGLQCPSAKGIIHLGA
                     TSCFVTDNTDIIIMKTALILIRKKLVSVIKNLSDFALKYKDMPTLGFTHFQPAQLVTV
                     GKRACLWIQDFCMDVDELEFVVDTLMMRGVKGTTGTQASFMSLFNDDENKVDELERLV
                     CEDFGFDKAFNITGQTYSRKTDSRVLKSLSSIAQSAHKMGTDIRLLQGLKEIEEPFET
                     NQVGSSAMAYKRNPMRSERICSLARYIITNAHNPEITASVQWLERTLDDSANRRLSIA
                     EGFLATDGMLDILINITDNLVVNENVIKKHIREEIPFIATENILMESVKRGGDRQLLH
                     EKIRQYSLIASKRVKEEGLDNNLLELISKDDVFSMNKEDLNNILNPSKYIGRSVSQVE
                     IYIKSIQNIFKKYYTGHIEVNLNV"
     gene            complement(3518..4792)
                     /locus_tag="PLQ68_09985"
     CDS             complement(3518..4792)
                     /locus_tag="PLQ68_09985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005600712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HXK73023.1"
                     /translation="MDFIKEYKKEKIRYEEFKNDPIKLDISRGKPSKKQVDYVASVFK
                     KAENEFDLYSNRTGLDKQDVGNYGLLPGITELRQLFGELLDLPYENIIIGGNSSLSLM
                     YDILSLNLMFGNMQSKTPWYNLENRKFLCPSPGYDRHFMMTEYLGFELITIPMTPHGP
                     DMDMVESLVKSDPMIKGIWCTPLYSNPDGYVYSDETVERLAKMSTAASDFRIFWDNAY
                     FTHHLVPNKVNKIKNIIKLCEKYNNADRVYQFSSTSKISFPGAGVSLIASSKTNIEHI
                     QNNFKYRIICYDKINMFRHAIVFRTKADVDAHMEKLAEFNRPKFNSVLEILDDELSEF
                     KNYLHYTRPEGGYFISLYTMEGCAKRTYQLCKEAGLVITTVGDTYPYGKDAKDSNIRI
                     APTFPELDELNKSISLLACCIKIAILEKLTDN"
     gene            complement(4832..>5070)
                     /locus_tag="PLQ68_09990"
     CDS             complement(4832..>5070)
                     /locus_tag="PLQ68_09990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009528059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HXK73024.1"
                     /translation="LDINQTAVIHGDALSITIAAASIIAKVSRDRMMDEYDKIYPQYS
                     FAKNKGYGTKEHIDAIKKYGLSPIHRKTFVKNFI"
     assembly_gap    5071..5170
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5171..5394)
                     /locus_tag="PLQ68_09995"
     CDS             complement(<5171..5394)
                     /locus_tag="PLQ68_09995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020814328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK73025.1"
                     /translation="MSFSKLKNHFFSNTLDKCSILLEEHVGYDYYEKSRNLLNNETDR
                     LHKLRKYENFLVQAGNKYIAGVDEAGRGPLA"
     gene            complement(5384..6235)
                     /gene="ylqF"
                     /locus_tag="PLQ68_10000"
     CDS             complement(5384..6235)
                     /gene="ylqF"
                     /locus_tag="PLQ68_10000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015534986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HXK73026.1"
                     /translation="MNINWYPGHMVKAKRMITENLKLVDLVVEILDARLPYSSRNPNF
                     EKLFNNKKKVIVLNKYDLADPSVTTQWQKYYESSDIEVITVNSKTGSGNKDFINAINK
                     VMAYKFEKDRLKGMKQRPVKAMICGIPNSGKSTFINKLTGSAPAKTGDKPGVTKDRQW
                     IKIHKNIHMLDTPGVLWPKFDDDKVALNLAITGAINDEILDLEQISRKLLSFLSEKYS
                     ENLKKRYDLNELNSDPHKLLLDICIHRNFIKSGGEPDTLKAAIIILDEFRAGKIGRIS
                     LEAPNEF"
     gene            complement(6324..6674)
                     /gene="rplS"
                     /locus_tag="PLQ68_10005"
     CDS             complement(6324..6674)
                     /gene="rplS"
                     /locus_tag="PLQ68_10005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015924260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HXK73027.1"
                     /translation="MDIVKSIEQSMIRTDLPDLQIGDFLKVNLKVKEGNRERIQSYEG
                     TLIARKGRGLTETITVRRISYGVGVERIIPLNSQKIASIEVIRRGQVRRAKLYYLRDR
                     VGKAAKIKEKLSVK"
BASE COUNT         2169 a         1057 c          931 g         2534 t
ORIGIN      
        1 gttgtatctg ttcattgtcc gctaaatgag cagaccttag gaatgtttaa tcaggatgca
       61 tttaataaaa tgaaaaagaa tgcaatgttc ataaatacag ccagaggcgc tatagttaat
      121 gaaaagtctc taagaaatgc tcttgaaaac aatacaattg catcagcagc tatagatgtt
      181 cttgaaactg aacctatgga taaagactgt attttatata atgtaaaaaa tctgataata
      241 acaccacata ctgcatggat accatatgaa acaagaaaga gattgcttgg tatagtagta
      301 agcaatttga agaactttat tgatggtaat ccaacaaatg tagtttcaaa ataataggaa
      361 aattaaaata agaacgattg ttcctttgct taataaagca atataagaaa aaaatagtat
      421 atttatatag tatttaaata ttttcattta ttttatactg ttaatcaact tatatgtagc
      481 attgaatcaa taagatcaac gaaagtgatt aatgattagt gattaatgta taaatattga
      541 actaaatgtc taatcttata agtttatttg atgctagcta atgggttatt aatcaatatt
      601 caatattgtg gatttaaatc taattattag gacaagtaaa tgtttaatat aagaaaagat
      661 aataataatt gtaaacattt gataacatga atgttcagaa tatttttatg taataaaaag
      721 gctatagatt gatttaaaaa atcataatcg tataattcat caagtaagca taaataaatc
      781 aatgtataga ttcaaaaaaa ataacaaatt agatattatt aaactgtaca aacatttacg
      841 tttaatgttt gagaattaat gagatctgta tgagtgaata atttataaac caagttatag
      901 tacaaatgta tattttgtat gacgatatta aatgagcgta aacgaaaata aaaaagatgt
      961 ataaaattaa aataataata ttagatagga ataaaaaaat aaaataagca atataattat
     1021 atcatttatt taaaagtgtt tcgaaatcga gtgagtaaga aaagacgtaa aaaagaatca
     1081 aataggccaa ttttagacaa aactactaat tgatgctact ctgtattaga caaaattatc
     1141 attatgtcta atcaataaat ttattttaac acctaatttt atctatattt cctgcccttt
     1201 gcatgtgttt agtgattcaa tttatgttgt tacattaaaa atgacaaata ataattttta
     1261 taatatattt gtaaatgagt gttgaatata aatattttta aaaaactata ctcttcttga
     1321 cctgttgaat tctattactg aattatatgc gctgtagtat gttgtgttat attcaactac
     1381 attaccggtt cttccatgca acagtgtttc aattttaaac aatggtgcat tttcaggtac
     1441 tttaattatt ttacacatgt cagaatcagc attaacaagt ttaattgatg attttgcatt
     1501 agttggatcc aaaggatact tccctactag taggtctact gaattaacat ttttaaataa
     1561 tagctcagca gcattgggaa aatagttaag tggtaatatt gatatagaat atgcaatatt
     1621 attatctgtt tcagtagtgt aagttacttt tgtataatct tcgtgataat ttgatttata
     1681 atcagtacta aattttctat tgagttcaag tgaaccatta attgttgtag attctataat
     1741 aactcgattt gaagtgttaa ttgtgtctaa aatacaatga ataaaacctt ttgtatgatc
     1801 ataggtcgtt ttattaacaa aatctgctac aaaagtgcct tttcctttaa tcttaataag
     1861 atatcctatc ttagctagct caccaattgc ctgtctgaca gtaggtcttg aaagactgta
     1921 taaatctgca agctcgtttt cagatggtat cacttcacca ggagcatatt tgccttcaat
     1981 gatagatttt attaattctt ctttaagctg gtagtataaa ggcgttgagc tatatttatt
     2041 aatcatttca ttcacctgtc cttacaagat gaatacatag tatcatacat ttagatttac
     2101 ttcaatatgt ccagtgtaat attttttaaa tatattttgg atgcttttta tataaatttc
     2161 aacttgtgaa accgatctgc ctatatattt tgaaggattt agaatgttat ttaaatcttc
     2221 tttattcata gaaaaaacat catctttgga tattaattcc agtaaattat tatcaagccc
     2281 ctcttctttt actctttttg aagcaatcaa agaatattgc cttatctttt catgaagcag
     2341 ctgtctgtcg cctccccttt ttacactttc cattaatata ttttctgtag cgataaaagg
     2401 aatttcttct ctaatatgtt tttttataac attttcattt actactagat tgtctgtaat
     2461 atttattaat atatctagca ttccatctgt agctagaaag ccttcagcta tagataagcg
     2521 cctgtttgca gagtcatcaa gggtacgttc aagccattga acagaagctg taatttcagg
     2581 gttatgtgca tttgtaatga tatatcttgc taatgaacag atacgttcgc ttctcattgg
     2641 atttcgctta tatgccattg cggacgatcc aacttgattg gtttcaaatg gctcttcaat
     2701 ttctttgagt ccctgaagca aacgtatatc agtacccatt ttatgagcag actgagcaat
     2761 tgaagaaagt gatttcagga cacgagaatc tgtttttctt gaataagtct gacctgtaat
     2821 attaaaagct ttatcaaatc cgaaatcttc acaaacaagt ctctcaagtt catcgacctt
     2881 attctcatca tcattaaata aagacataaa gcttgcctgt gttcctgtag ttccttttac
     2941 tcctctcatc atcagtgtgt ctactacaaa ttcaagttca tcaacatcca tacaaaaatc
     3001 ctgtatccat aaacatgctc tctttccaac agtaacaagc tgtgcaggtt gaaaatgagt
     3061 gaaacctaat gtaggcatat ctttatattt aagagcgaaa tctgataaat tcttaattac
     3121 gcttacaagt ttttttctta taaggataag cgcagttttc attattatta tatcagtgtt
     3181 atcagtaaca aaacatgaag ttgctcctaa atgtattatt cctttagctg atggacactg
     3241 taaaccatag gcttttatat ggctcattac atcatgtcta atttgctttt caaataattt
     3301 aacctgatca taatcaatat tatctttatt ctcttcaagt tcatcaattt gtttttttgt
     3361 tataggaagg cctaatttca tttgggcttt tgctaaagaa atccataatt ttctccatgt
     3421 tgaaaattta taatcatctg aaaaaattaa ttgcatttct tttgaactat atctttgaga
     3481 aaaaggactt gtataattat cactcatctt cattacctca attatccgtt aatttttcta
     3541 aaatagcaat ttttatgcaa catgctaaaa gtgaaatgga tttattcaat tcatcaagtt
     3601 caggaaatgt aggtgcaata cgtatgttgc tgtctttagc atctttacca tatggatatg
     3661 tatcaccaac agtagttatt acaagaccag cttctttaca taactgatat gttctttttg
     3721 cacaaccttc cattgtgtat aaagatatga aatatcctcc ttcgggtctg gtgtagtgta
     3781 gataattctt gaattctgat agctcatcat caagtatttc taatacagaa ttaaattttg
     3841 gcctgttaaa ttcagcaagt ttttccatat gcgcgtctac atcagctttt gttcgaaaaa
     3901 ctattgcatg tctgaacata ttaattttgt cataacagat aattctatac ttaaaattat
     3961 tctgaatatg ctctatgtta gtctttgatg acgcaataag agaaactcca gctcctggaa
     4021 aagatatttt acttgttgat gaaaactgat atactctgtc agcattatta tatttttcac
     4081 acaacttaat tatattctta attttgttga ctttatttgg aacaagatga tgtgtaaaat
     4141 atgcattatc ccaaaatatt ctgaaatcag aagcggctgt agacattttt gctagtcttt
     4201 caactgtttc atcagaatac acatatccat caggattact atataaagga gtgcaccaga
     4261 taccttttat cattgggtcg gatttaacca gtgattcaac catatccata tcaggaccat
     4321 gtggtgtcat tggaatagtt atgagttcaa atcctaaata ttcagtcatc atgaaatgtc
     4381 tgtcatatcc tgggcttggg catagaaatt ttctgttttc aagattgtac catggggttt
     4441 ttgactgcat atttccaaac ataaggttta aagatagaat atcatacatt aaacttaaac
     4501 tggaatttcc accaattatt atattttcat aaggcaaatc aagcaattca ccgaaaagct
     4561 gacgtaattc agttatccct ggtaaaaggc cgtaattacc gacatcttgc ttatctaaac
     4621 ctgttctatt tgaatataaa tcaaattcat tctctgcttt tttgaaaaca gaagcaacat
     4681 aatcaacctg ctttttacta ggtttacctc ttgaaatgtc aagtttgata ggatcattct
     4741 tgaattcttc atatcttatt ttttcttttt tatactcttt tataaaatcc attttaattt
     4801 cctttttcat tttcggctaa ttatatcata tctaaataaa atttttaaca aatgtttttc
     4861 tgtgtatagg tgataatcca tactttttta tagcatcaat atgctcctta gtaccataac
     4921 ctttattttt cgcaaatgaa tactgaggat aaattttatc gtattcatcc atcatcctgt
     4981 ctcttgatac ctttgcaata attgacgctg cagcgattgt tattgaaaga gcatctccat
     5041 gaataacagc tgtctggttt atatcaagat nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5161 nnnnnnnnnn gctaatggtc ccctgcctgc ttcatctact ccggcaatat atttattgcc
     5221 agcttgcact aagaaatttt cgtattttct taatttatgc agtctgtctg tttcattatt
     5281 taataaattt cttgattttt cataataatc gtaacccaca tgttcttcaa gtaatatact
     5341 gcatttgtct aaagtatttg aaaagaaatg attttttaat ttactaaaac tcatttggtg
     5401 cctcaagaga aattcttcct attttaccag cacggaattc atctaagata atgatagcag
     5461 ccttaagagt atctggttct cctcctgatt ttataaaatt cctgtgtata catatgtcta
     5521 gtaataattt gtgaggatct gaattaagtt cattcaaatc atatcgtttt tttaggtttt
     5581 cactatattt ttcacttaaa aaagataata attttctgct tatttgctct aaatctaaaa
     5641 tttcatcgtt tatcgcacct gtaattgcaa gattcaatgc tactttatcg tcatcaaatt
     5701 taggccatag aacaccagga gtgtcaagca tatggatatt tttgtggatt ttgatccatt
     5761 gtctgtcttt tgttactcca ggcttatcac ctgtctttgc tggcgcagat ccagtaagtt
     5821 tattgataaa ggtggattta cctgaatttg gtattccgca tatcatagct ttaacaggtc
     5881 tttgcttcat tccttttagt ctgtcttttt caaatttata agccattacc ttattgattg
     5941 cattaatgaa atctttattt cctgaacctg ttttactatt aactgtaata acctcaatat
     6001 cagatgattc gtaatatttc tgccattgtg ttgttactga gggatcagca aggtcatatt
     6061 tgtttagtac tattactttc tttttattgt tgaataattt ttcaaaattt ggatttctac
     6121 tggaatatgg taaccttgca tcaagaatct caactacaag atccacaagt tttaaatttt
     6181 cagttatcat ccttttggct ttcaccatat gtcctggata ccagttaata ttcataattt
     6241 tccttactta atattctact tataattata tagtatagtt tatcttaaat gaaataaaaa
     6301 aacgggacta agtcccgtat tttttactta acactaagtt tttcttttat tttggctgct
     6361 tttcctactc tatctcttaa atagtaaagc ttagcgcgtc tgacctgtcc tcttcttata
     6421 acttcgatag aagcgatttt ctgagaattt aaaggaatta tcctttcaac acctacacca
     6481 tatgaaattc ttcttacagt tatggtttct gttaatcctc ttccttttct tgcaatgagt
     6541 gtaccttcgt atgactgaat tctttctcta ttgccttcct taacctttaa gttaacttta
     6601 aggaagtctc ctatctgaag atctggcaag tctgttctta tcatgctttg ttctattgat
     6661 ttgactatat ccattttata atcctccctt catcttagcg ttcatgtatc aaatttgatt
     6721 acagtggacc gccctttacg aacctgcagt attgtatcat atgcaaagta atatgtaaat
     6781 aagattttca a
//
LOCUS       DAOSXM010000105         6674 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2111
            NODE_7492_length_6674_cov_10.126001, whole genome shotgun sequence.
ACCESSION   DAOSXM010000105 DAOSXM010000000
VERSION     DAOSXM010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864178
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 6674)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6674)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:24:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,261
            CDSs (total)                      :: 2,229
            Genes (coding)                    :: 2,218
            CDSs (with protein)               :: 2,218
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 3 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6674
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7492_length_6674_cov_10.126001"
                     /isolate="AMR_MDS_2111"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..674)
                     /gene="amrB"
                     /locus_tag="PLQ68_10010"
     CDS             complement(<1..674)
                     /gene="amrB"
                     /locus_tag="PLQ68_10010"
                     /inference="COORDINATES: protein motif:HMM:NF014899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HXK73028.1"
                     /translation="MSIIAAFIFPHPPIIMPSIGKGEEKKIRKTSDCYKKACEMIAKL
                     KPDTIIISSSHCSMYSDYIHISSADEFNGSLSRFGAKGISVHAEYDKEFISVLKEETE
                     GVNLNAGLLGYEEPDSDHASVIPLAFLNEFYTDYRLVRMGISHLPFAEHYRLGMCISE
                     VSEKLNRRSVFIASGDLSHKLKVFGPYGYAKEGEQYDKIVTEAMSKGNFSKFMDLKED
                     FLEKAA"
     gene            complement(717..>1131)
                     /locus_tag="PLQ68_10015"
     CDS             complement(717..>1131)
                     /locus_tag="PLQ68_10015"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HXK73029.1"
                     /translation="FVLAIFDIDGFKMINDKFGHDVGDTALKIVAERSSEFFKDKGFV
                     ARFGGDEFAILFSDADEEYLEVLKKFKEFVDSSDIGQYKYSFTISIGAAVFPVDSDDH
                     DVLIKYADDALFYVKKNGKNGIAFYSDINLNKSSM"
     assembly_gap    1132..1231
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1232..2058)
                     /locus_tag="PLQ68_10020"
     CDS             complement(<1232..2058)
                     /locus_tag="PLQ68_10020"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HXK73030.1"
                     /translation="MIFGIQTMVFLVCFIILTQEWIQHRNRYKGLSFWVAMMICGFAG
                     YTLIALRGTIPDFLSIIVANSLNILAFALFYTGLIFFFKVKAHYLHNIFIVITAILVF
                     IYFTYINPSLTVRIITINITYIIIFSQALYLFICKIKTCQKSMSYIYIINISALIILN
                     IYRTIMVFLNNSGNQDFFNQSIHEKIYFIINMIVMMYLMINLAMLVSRRLLHDIGEKE
                     EKFNTIFHSAPYAALITEAETGIILEVNKEMLDLLGYERSEVEGRTVFDLEFYPDAS"
     gene            2251..3336
                     /locus_tag="PLQ68_10025"
     CDS             2251..3336
                     /locus_tag="PLQ68_10025"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF017509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HXK73031.1"
                     /translation="MNKKRTILLIISIVLITALLFTGCNSQDQKFTYSRDIDNNGYWR
                     GIKASNYVELGSYIGLSIPKDTYTVTEDELQSNLTSILQDHLVKTDVYDRDVVYGDTL
                     NIDYIGRVDGVAFEGGSTGGNGADVTIGVTNYIDDFLEQLIGHMPKETFDIEVTFPED
                     YGDEQLNGKDAIFTITINHIVESSIPELTDEFVNATFSESNGWTTVEQMKSGLTENIK
                     DTKVSEYIQDYLTANSTIKEIPQMILDFQDEAIILFYQNYATQYQMELDDFLKQYVGV
                     ENQQQLLEQYKEDNEKVSEMHLVIQAVAEDAKIKATKDDVQNYFENYMQTDDYSQYEE
                     IYGLNYLKLTTLVQMVLDHIQDNAVFE"
     gene            complement(3378..4031)
                     /locus_tag="PLQ68_10030"
     CDS             complement(3378..4031)
                     /locus_tag="PLQ68_10030"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008424110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HXK73032.1"
                     /translation="MEIDIKIRQVQPRDYHAVEELTREAFWNHYVPGCDEHYLMHIIH
                     QSDSYIPQLDMLALIDDKIVGNIVYTKANVLMDNDELYDVITFGPISVLPEYQKKGIG
                     GRLIKHTIKLAEKLGHKAILIYGDFDYYCRYGFIQAEKYKIGTSHNTYIPSLQAYEIV
                     KGYLKPGRFFEDKIYDIDEEKAKIFDKNFPFKKLKEGLESQKRFSYLIGLSKPRRIE"
     gene            complement(4036..4923)
                     /locus_tag="PLQ68_10035"
     CDS             complement(4036..4923)
                     /locus_tag="PLQ68_10035"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HXK73033.1"
                     /translation="MKKECSIIRDILPLYTENMVSDETREFIEEHLSVCNECKKEMEK
                     MRIIDNMPPSNTVPLKNIRKKLLYKKIQIVLLTTALVLIIAISVFALLTAPKFYPYSD
                     DILKLTENQNQSVTITFREDVKGFSYIESSDIDTDISVVQVEAWTSYFSENILKSSRQ
                     SITIPYTDKDKISIYYLQNNDEEDVHIFGHNLLPTGKIVSLPRLALNYYLYVAIGSFV
                     LAVLLLVVFRKKEMIRIWLLRLMAIPVSYVLGHIFIKGFSTTTYTLQRDFFLILLVSV
                     MIYGVIISSIWLYNMKKKR"
     gene            complement(4920..5414)
                     /locus_tag="PLQ68_10040"
     CDS             complement(4920..5414)
                     /locus_tag="PLQ68_10040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019678091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HXK73034.1"
                     /translation="MTEFEKIYRTYFNDVYLYILKLSGNEIIAEEITSETFFKALHAI
                     DNFKNECDIRVWLCQIAKNNYFTYLKKHKKYVDLDQLNEADNMQWHNPVIEKMEDQSD
                     SIMIQEILHELDEPYKEVFMLRVLGELSFKQIGHIFKKTDNWACVTYHRAKNMIIEKM
                     EEKK"
     gene            complement(5563..6663)
                     /locus_tag="PLQ68_10045"
     CDS             complement(5563..6663)
                     /locus_tag="PLQ68_10045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004605893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HXK73035.1"
                     /translation="MSWKNDLSKNITKAEELKLYLQLSEEKTKKISQVTDKFPMSIPH
                     YYLSLIDKSNEEDPIRRMCVPSGYEEDTCGSFDTSGETENTVISGLQHKYKQTALILS
                     TNNCAMYCRHCFRKRLVGLDDDEIAKNIKPMTDYISSHEEISNVLITGGDSFMLSNTR
                     IEDYLKALTKIGHLDFIRFGTRTPVVLPKRITEDDELIEILSRYCKIKQIFVVTQFNH
                     KNEFTDESKKAIKKLINHNIPVLNQTVLLKGVNDSAESLADVFKELTSAGAIPYYLFQ
                     CRPVSGVKDRFQVPFIRAYEIVEGAKKLQNGIGKSFRYCMSHVTGKIEILGPEGDKML
                     FKYHEAKYDKDFNRIFSLKIDKDTCWIGDDEI"
BASE COUNT         2170 a         1172 c          924 g         2308 t
ORIGIN      
        1 tcagctgctt tttccaaaaa atcttccttt aaatccataa attttgagaa attccccttt
       61 gacatagctt cagtgacaat cttatcatac tgttcacctt ctttagcata gccataagga
      121 ccaaacactt ttagtttatg tgacagatct ccactggcaa taaatacaga tcttctatta
      181 agtttttcag atacttccga aatacacatt ccaagacggt agtgctccgc aaaaggaaga
      241 tgtgaaatac ccatcctgac aagccgataa tctgtataga attcattcaa aaaagctaaa
      301 ggtattacac ttgcatggtc actatcaggc tcttcatatc ctaataatcc agcatttaga
      361 ttaactcctt ctgtctcttc ttttaaaaca gatataaatt ctttatcata ttctgcatgg
      421 actgaaatgc ctttcgctcc gaaccgggat aaactgccat taaattcgtc tgccgatgat
      481 atgtgaatat agtcagaata cattgaacaa tgcgatgaag atataattat agtatcaggt
      541 tttaattttg ctatcatttc gcaggctttt ttgtaacagt ctgacgtttt tcttatcttt
      601 ttttcttcac cctttccaat tgatggcatg attataggag gatgcggaaa tatgaatgct
      661 gctattattg acatagtaca ccccctctta aactttaact gtatttaaat tatatttcac
      721 attgaagatt tattcaagtt tatatcagaa tagaatgcaa taccattctt cccattcttt
      781 ttcacataga agagagcatc gtctgcatac tttataagta catcatgatc atctgagtcc
      841 acaggaaaga cagctgctcc tatacttata gtaaatgaat acttatactg tcctatatct
      901 gatgaatcaa caaattcttt gaattttttc agaacttcta aatattcttc gtctgcatca
      961 gaaaatagta tagcaaattc atcccctccg aatctggcta caaaaccttt atctttgaaa
     1021 aattcacttg acctttctgc tactatcttt agagcagtat cgcctacatc atggccgaac
     1081 ttatcattta tcattttaaa tccgtctata tcaaaaatcg ctaaaacaaa tnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nctgcttgca tcaggataga actcaaggtc
     1261 aaatacagtt cttccctcta cttcgcttct ttcatatcct aaaagatcta acatttcttt
     1321 attcacttca agtatgattc cagtttctgc ttctgtaatt aaagcagcat atggtgcact
     1381 atggaaaatt gtattaaact tttcttcctt ttcacctata tcatgcagca atcttctaga
     1441 tacaagcatt gcaagattta tcatcagata catcataact atcatattga ttatgaaata
     1501 gattttttca tgtatgctct gattaaagaa atcctgattt ccagaattat tcaaaaatac
     1561 cataattgtt ctgtaaatat ttagaattat gagagcagat atattaatga tgtaaatata
     1621 tgacatgctt ttctgacatg ttttgatttt acatataaat agataaagag cctgtgagaa
     1681 tatgattatg taagttatat taatcgtaat aattctaacg gtaagacttg ggttgatata
     1741 tgtgaaatat ataaaaacaa gaatggcagt aattacaata aatatgttat gaagatagtg
     1801 tgctttaact ttaaaaaaga atataagacc agtgtagaat aatgcaaatg ccaaaatatt
     1861 taatgaattt gcaactataa ttgataaaaa gtcaggaatt gttcctctta atgcaattaa
     1921 tgtataacct gcaaaaccgc atatcatcat tgctacccaa aatgaaagac ccttgtatct
     1981 gtttctatgc tgtatccact cctgagtaag aattatgaaa catacaagaa aaaccatagt
     2041 ctgtattcca aaaatcatac gcatatccat taattatatc taccccgtta actatttatt
     2101 tccaagcaat tatactataa cttaataata aattcattaa aatatttgaa acacacaaat
     2161 tcatcactta tttgtgagat aatatctaaa aattataata tcacatggta taatgtgttt
     2221 tatgatatta agtaggagca tggagggata atgaataaaa aaagaacaat tttgctaata
     2281 atttcaattg tattaataac tgctttactt ttcactggct gcaacagtca ggatcagaag
     2341 ttcacataca gcagagacat tgataacaat ggatactggc gaggcataaa agcttcaaat
     2401 tatgttgaat tagggtcata tatcggctta tcaatcccaa aagatacata tacagtaact
     2461 gaagatgaac ttcagtcaaa cctgaccagt attttgcaag atcatttagt taagacagat
     2521 gtttatgaca gagatgttgt ttatggagac acacttaata tagattatat cggcagagtc
     2581 gatggtgtag cttttgaagg tggctctact ggaggaaatg gagcagatgt cactattgga
     2641 gtgacaaact atattgatga ttttttagaa cagcttatag gacatatgcc gaaagagact
     2701 tttgatattg aagtaacctt tcctgaagac tacggtgatg agcagcttaa tggcaaagat
     2761 gcaatattca ctattacaat taaccatata gttgaatctt caatccctga acttacagat
     2821 gaatttgtaa atgctacttt tagtgaatca aacggatgga caactgtaga acagatgaaa
     2881 tctggtttaa cagaaaatat aaaagataca aaagtgtctg aatatattca ggactattta
     2941 acagcaaact ctacaattaa ggaaatacct cagatgatac tggattttca ggacgaggct
     3001 attattctgt tttatcagaa ttatgcaaca caataccaga tggaacttga tgattttcta
     3061 aagcagtatg taggagttga aaaccaacag cagcttcttg agcaatacaa agaggataat
     3121 gaaaaggtat ctgaaatgca cctagttata caagctgtcg ctgaagatgc aaaaataaaa
     3181 gcaacaaaag atgatgtcca gaattatttt gaaaactata tgcagactga tgattactct
     3241 caatatgaag aaatatatgg tttgaattac ttgaaactta ctacattagt tcaaatggtg
     3301 ctggatcaca tacaggataa tgcagttttc gaataaaaat attaaacata ataaataaag
     3361 agcgtaagct ctttttttta ttcaatcctt ctgggttttg acaagccgat aagataactg
     3421 aacctctttt gcgattccaa accttctttt aatttcttaa atgggaaatt tttatcgaat
     3481 atttttgcct tttcctcatc tatatcatat atcttgtctt cgaaaaacct tcctggtttt
     3541 aaatagcctt taactatctc atatgcctgc aatgaaggta tatatgtgtt atgtgacgtt
     3601 cctattttat acttttcagc ctgaataaac ccatatctgc agtaataatc aaagtctcca
     3661 tatataagta ttgctttatg acctagtttt tctgccagtt taatagtatg tttaataagt
     3721 cttccgccta tccccttttt ctgatattca ggtagtacag atataggtcc gaaagtaata
     3781 acatcatata actcatcatt atccatcaat acatttgcct ttgtatatac gatattaccg
     3841 acaatcttat catcaattaa agctagcata tccaactgcg gaatataact atcactttgg
     3901 tgtattatat gcataagata atgttcatca catcctggaa cataatgatt ccagaaagct
     3961 tctctggtta gctcttctac tgcatggtaa tctctgggtt gaacctgtct gattttaata
     4021 tctatttcca ttttttcatc tcttcttttt catattatac aaccatatac ttgaaatgat
     4081 aacaccataa atcataacag aaactaaaag aatcaaaaag aagtctcttt gtaaagtata
     4141 tgtagttgtc gaaaatcctt ttatgaaaat atgaccaaga acatatgaaa ctggtattgc
     4201 cataagcctt aaaagccata tccttatcat ttctttttta cgaaatacta ctagcaataa
     4261 aactgctaat acaaaagaac caatggctac atataagtaa tagttaagag ctagccttgg
     4321 aagcgacaca atcttacctg ttggtaataa gttgtgtcca aatatatgaa catcctcttc
     4381 atcgttattc tgaagatagt aaatagaaat cttatcttta tcagtatatg gtattgtaat
     4441 actttgtctt gagcttttaa gtatattctc actaaaatag ctagtccatg cttcgacctg
     4501 gactactgag atatctgtat caatatcaga ggattcaatg taactgaaac ctttaacatc
     4561 ctctctaaat gttatggtaa cgctctgatt ctggttttcc gtcaatttta atatatcatc
     4621 tgaataagga taaaatttcg gggctgtgag aagagcgaat acagatattg ctattattaa
     4681 caccaaagct gtggttagaa gaactatctg aatcttttta tacaaaagct tttttcttat
     4741 attcttcaac ggcactgtat tacttggagg catattatca attattctca ttttctccat
     4801 ttcctttttg cattcattac atactgataa gtgttcctca ataaattccc tcgtttcatc
     4861 actcaccata ttttcggtgt ataaaggtaa tatatcccta attatgctgc attctttttt
     4921 catttctttt cctccatttt ttctattatc atattttttg cacggtggta tgtgacacag
     4981 gcccagttat cagttttttt aaagatatgt cctatctgct taaagcttaa ttctcctaag
     5041 actcttaaca taaacacttc tttatatggt tcgtccagtt catgtaagat ttcttgaatc
     5101 attatggaat cactttgatc ttccattttt tcaataaccg gattgtgcca ctgcatatta
     5161 tctgcctcat tgagctgatc taaatcaaca tattttttat gttttttcaa gtaagtaaag
     5221 taattatttt ttgcaatctg acataaccat actcgaatat cacattcatt cttaaagttg
     5281 tctattgcat gcaaggcttt aaaaaaagtt tcacttgtaa tttcctctgc aataatttca
     5341 tttcctgaca actttaatat atatagataa acatcattga aatatgttct atatatcttt
     5401 tcaaactctg tcacaactca cctccatata tatgactcag aaagacagca aatcttacaa
     5461 gtttttaaaa ataattaaat ttgttcgtgt taattatatc agcaaatagc tgaatcatat
     5521 attaatagaa tatataaaga aaataattat agtattaata ttttaaatct catcatcacc
     5581 aatccagcag gtgtctttat caattttcag tgagaaaatc ctattgaagt ccttatcata
     5641 ttttgcctca tgatatttga ataacatttt atcaccttcc ggaccaagta tttctatttt
     5701 gcctgtcaca tgagacatac aatagcgaaa actctttcca attccgttct gaagtttttt
     5761 agcaccttca acaatctcat atgcacgaat aaaaggaact tgaaatctat cctttactcc
     5821 actcaccggc cggcactgga ataaataata tggtattgct cctgctgatg tcagttcctt
     5881 aaatacatca gctaatgatt cagcgctatc attaactccc ttaagcagaa cagtctggtt
     5941 tagaacagga atattatgat ttattagttt ttttatagct ttttttgatt cgtcagtaaa
     6001 ttcattttta tgattaaact gggttacgac gaaaatctgc tttatcttgc agtacctact
     6061 tagaatttca atcagttcat catcttcagt tattcttttg ggtagcacta caggcgttct
     6121 tgtaccgaat cttataaaat caagatgacc aatcttcgtc aaagccttaa gatagtcttc
     6181 tattcttgta ttactcagca taaaagagtc cccaccagtt ataagtacat ttgatatttc
     6241 ctcatgtgaa gaaatgtaat ctgtcatcgg ttttatattc tttgctatct catcatcatc
     6301 caatcccact aaacgttttc taaagcaatg tctgcagtac atagcgcagt tgtttgtaga
     6361 aagtattaaa gctgtctgtt tatacttatg ctgaagaccg ctaataactg tattttctgt
     6421 ttcaccactt gtatcaaagc ttccgcatgt atcttcttca tatcctgatg gaacacacat
     6481 tcttctaatc ggatcctctt cattcgactt atcaatcaat gataaatagt aatgaggtat
     6541 agacatagga aatttatctg ttacttgtga tatctttttt gtcttttctt ctgacaactg
     6601 caaatataac ttaagctcct cagctttagt aatatttttt gataaatcat tcttccaact
     6661 catttttacc ttct
//
LOCUS       DAOSXM010000106         6584 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2111
            NODE_7634_length_6584_cov_8.263134, whole genome shotgun sequence.
ACCESSION   DAOSXM010000106 DAOSXM010000000
VERSION     DAOSXM010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864178
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 6584)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6584)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:24:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,261
            CDSs (total)                      :: 2,229
            Genes (coding)                    :: 2,218
            CDSs (with protein)               :: 2,218
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 3 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6584
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7634_length_6584_cov_8.263134"
                     /isolate="AMR_MDS_2111"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1024)
                     /gene="rseP"
                     /locus_tag="PLQ68_10050"
     CDS             complement(<1..1024)
                     /gene="rseP"
                     /locus_tag="PLQ68_10050"
                     /inference="COORDINATES: protein motif:HMM:TIGR00054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HXK73036.1"
                     /translation="MSTFTSILIALLALSIIIIIHELGHFIVAKASGIKVLEFGLFMG
                     PKLFSKTINGTIYSIRAIPIGGFVAMEGEDENVISPTSYSSAKPYKKILVSVAGPFFN
                     IVFAFLILTILFSFTGYALDSNTVIEENGPLYYAGLRDGDKIVKIDDRKILDPSDITI
                     ALYPLQENETVDVIYEREGVRNIVNVEPVKKGGVRIMLNITTQSTDTIVVDIDENSNG
                     YIAGLRSNDKITHINDQPVNSLNELSSLVNENGIGSIKVTVERGQDQLSFVFDTIESE
                     SMEYYDLGLYYYSSKINVFQAIKHSVLYSISVVKTVIISIVWLITGTVKVSEMMGPVG
                     IISTIGE"
     gene            complement(1021..2166)
                     /gene="dxr"
                     /locus_tag="PLQ68_10055"
     CDS             complement(1021..2166)
                     /gene="dxr"
                     /locus_tag="PLQ68_10055"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: protein motif:HMM:TIGR00243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HXK73037.1"
                     /translation="MKICILGATGSIGENTIKVCKNLGYNIVGLTCKTNYSKLAEIIS
                     FYNLENSLKFIGISDNFSMNQFKTLGLKMSHKVFGGNDANIDCIVSSQADIIVNALVG
                     SEGIKPSFVSLSNCKRLALANKETIVAAGDIILNEAKKNNCEIIPIDSEHGAILQCLE
                     SNPIKKIYLTASGGPFWRLPSEQFDKITIEQALNHPNWKMGRKITIDSATMMNKGFEV
                     IEASKLYSLESKKIDVLIHPSSIVHSIVEYYDNSMKAQLSTCDMKIHIQYALTYPKRA
                     SINYEQLDLTKQPLEFFKIDLEKFKCLKLAYMAVEEGGTIGTVINAVNDVCVEAFLNG
                     KIKFTDIASIIERVTNEYDNKQAENIDHILEIDSYAKTKAKILEENI"
     gene            complement(2166..>2813)
                     /locus_tag="PLQ68_10060"
     CDS             complement(2166..>2813)
                     /locus_tag="PLQ68_10060"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HXK73038.1"
                     /translation="FMYYSNLYLTLGLSIVPKEYLYTINILITIFFALTILVASVFYK
                     IKTFNEVSYTIFGFLYTVILFSFIILILDMEKGGFYFVYVWLGALGCDLWAFLIGRTF
                     GKTKIIKDVSPNKTIEGSIGGALGSILMISIYTIIVNRFFNAQIPWYVLVAMFIPCGL
                     FSQFGDWCASFIKRQFSIKDFGKIMPGHGGALDRLDSILFITPSVYLILLLAGLR"
     assembly_gap    2814..2913
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2914..3512)
                     /gene="uppS"
                     /locus_tag="PLQ68_10065"
     CDS             complement(<2914..3512)
                     /gene="uppS"
                     /locus_tag="PLQ68_10065"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HXK73039.1"
                     /translation="MQLPQHIGIIMDGNGRWAKQKKMPVSYGHKCGADAIQTLIEESD
                     KLGIKYITAYTFSTENWKRPKSEVNYLMSLLQEFLNDTSGRLEKSNIRIRFIGSRDKL
                     KKSMIKSMEKIEKISKHKSGLNFIIALNYGARQEIADAVKNICNDVCSKNLDINNINE
                     DLIKEYLYTKDIPDPDLIIRPSGEQRLSNFLLYQASYSEL"
     gene            complement(3542..4063)
                     /gene="frr"
                     /locus_tag="PLQ68_10070"
     CDS             complement(3542..4063)
                     /gene="frr"
                     /locus_tag="PLQ68_10070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017437056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HXK73040.1"
                     /translation="MSQAIDAFSEHISTIRAGRANPSVLNNIYVDYYGQKTPLNQIGN
                     VSSPEPRLLVIQPWDTSILKEVEKAIYASDLGINPQSDGRVIRLIFPVLSQERREELV
                     KKVKTYGEEAKVKIRNIRRDDIEKYKKQRKDSEITEDDYTIIEKDIQTLTDEHITKID
                     DIVKNKTDEIMEV"
     gene            complement(4155..4856)
                     /gene="pyrH"
                     /locus_tag="PLQ68_10075"
     CDS             complement(4155..4856)
                     /gene="pyrH"
                     /locus_tag="PLQ68_10075"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HXK73041.1"
                     /translation="MNYKRILIKLSGEVFSGNNGGNIDMETLMDIAKALKQIKNDGKE
                     IAIVVGAGNFVRGRDIKDIVRSRADSMGMLATIINSIALKDALEKTGLDSEVYSAVLM
                     SEICKTYRRDDAVRDLKNGIITILAAGTGNPYFSTDMAAALRGIEINADVILFAKNVD
                     GVYDKDPKKHKDAIKYDRVTYSKILSEQLTVIDMAAAVLCDEFSMSSVLFALNEPKNI
                     IEASRGGNIGTIIEK"
     gene            complement(4901..5548)
                     /gene="tsf"
                     /locus_tag="PLQ68_10080"
     CDS             complement(4901..5548)
                     /gene="tsf"
                     /locus_tag="PLQ68_10080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010252267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HXK73042.1"
                     /translation="MISASAVKELREKTGVGMMECKKALVESNGDFAKAVDILRERGL
                     ANAQKKSGRIAAEGVVGSYIHGGGRIGVLVEVNIETDFAAKNEDFLAYVKDIAMQIAA
                     SKPEYVRREEIPAEIIEKEKAILKSQALNEGKPEKVVEKMIEGRIEKFYKQVCLLDQA
                     YIRNPDISVQQMTNEIIAKIGENISVRRFTRYEMGEGLQKREENFADEVAKQISK"
     gene            complement(5703..6506)
                     /gene="rpsB"
                     /locus_tag="PLQ68_10085"
                     /pseudo
     CDS             complement(5703..6506)
                     /gene="rpsB"
                     /locus_tag="PLQ68_10085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002838265.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
BASE COUNT         1954 a         1231 c          763 g         2536 t
ORIGIN      
        1 gttctcctat agtagatata ataccaacag gtcccatcat ttctgatacc tttactgttc
       61 cagtgattaa ccatacaata gatataataa cagttttaac tactgatata gaatatagta
      121 ctgaatgttt tattgcctga aatacattta tctttgaact gtaatagtat aatcctaagt
      181 cataatattc cattgactct gattcaatag tatcaaaaac aaaagataat tgatcttgtc
      241 ctctttcaac agttactttt atagagccaa ttccattttc attaacaaga ctacttaatt
      301 cgttcaaact attaacaggc tgatcattta tatgtgttat tttatcatta gatcttaatc
      361 cagctatata tccattcgag ttttcatcga tatcgacaac tatagtatca gtactttgag
      421 ttgtaatatt gagcataatt cttactccgc cttttttgac aggttcaaca tttacaatat
      481 ttcttactcc ttctctttca taaattacat caacagtttc gttttcttgc agcggatata
      541 aagctattgt aatatcagat gggtctaata tctttctgtc atcaattttt actatcttat
      601 caccatctct taaacctgca tagtataaag gcccgttttc ttcaatcaca gtatttgaat
      661 ctaatgcata cccagtaaaa ctgaataata tagttaatat aagaaaagca aatactatat
      721 tgaaaaatgg gcctgctact gatactagta tttttttata aggttttgca gaggaataag
      781 aagtagggct tatgacattt tcatcttccc cttccattgc aacaaaacca cctatgggta
      841 ttgctcttat actataaatt gttccattaa tagttttgct aaagagctta ggtcccataa
      901 aaagaccaaa ctcaagaacc ttaatgccac ttgctttagc aacaatgaaa tgtcctaatt
      961 cgtgaattat aattattatg cttaatgcta ataatgcaat taaaatactt gtaaatgtac
     1021 tcatatgttt tcctccaata ttttagcttt tgtttttgca taactgtcta tttctagaat
     1081 atgatcaata ttttctgctt gtttattatc atattcatta gtaactcttt ctattatact
     1141 ggcaatatca gtaaatttaa tttttccatt taaaaaagcc tcaacacata catcattaac
     1201 tgcatttata actgttccaa tagttccacc ttcttcaacc gccatatatg ctaattttaa
     1261 acatttaaac ttttcaagat cgattttgaa aaattctaaa ggttgtttcg taagatcaag
     1321 ttgctcataa ttgattgaag ctcttttagg ataggttaaa gcatattgta tatgaatttt
     1381 catatcacaa gtacttagtt gagctttcat actattatca tagtattcta caatagagtg
     1441 aacaatacta gatggatgaa tcaatacatc aattttttta ctctctagtg aataaagttt
     1501 tgatgcttca ataacttcaa atcccttatt catcatagtt gcactgtcta tagttatttt
     1561 tcttcccatt ttccaattgg gatggtttag agcttgttct atcgttatct tatcaaattg
     1621 ttcggatgga agtctccaaa aaggtcctcc gcttgcagta agatatattt tttttattgg
     1681 attactttca agacattgta atatagcacc atgttcagaa tctatcggaa ttatttcaca
     1741 attatttttt ttagcctcat tcaatataat atcgcctgct gcaactatag tttctttgtt
     1801 tgcaagtgca agtcttttac agtttgaaag tgagacaaat gatggcttta ttccttcact
     1861 tcctactaat gcgttaacta taatatctgc ttgtgatgat actatacagt ctatattagc
     1921 atcattacct ccaaaaactt tatgactcat ttttaagcct aatgttttaa actgattcat
     1981 gctaaaatta tcagatattc caataaactt aagtgaattc tcaagattat aaaaggagat
     2041 aatttctgct aatttggaat aatttgtttt acaagtcaag cctacaatat tataacctaa
     2101 atttttacat acttttatag tattttctcc tatggatcct gttgctccta aaatgcaaat
     2161 tttcattatc ttaatcctgc tagtaataat attaaatata cacttggtgt tataaatagt
     2221 attgaatcaa gtctgtcaag tgctccacca tgtcctggca ttatcttacc aaaatcttta
     2281 atactaaatt gtctttttat aaatgatgca caccagtctc caaactggct gaataatccg
     2341 caaggaataa acatagcaac taaaacatac catggtatct gagcattaaa gaatctattt
     2401 actataatag tatatataga tatcatcaga attgatccta atgctccacc tatagaacct
     2461 tcaatagtct tgtttggact tacatccttt attatctttg ttttaccaaa tgtccttcct
     2521 attaaaaatg cccataaatc acaacctaat gctcctaacc aaacatagac aaaataaaat
     2581 cctccttttt ccatatctaa aattaaaata ataaaggaaa ataagattac tgtgtataag
     2641 aaaccgaaga ttgtatatga aacttcatta aaagttttta tcttataaaa tacagatgct
     2701 actaaaatcg ttaaagcaaa gaatattgtt attaaaatat tgattgtata caaatattct
     2761 ttgggtacta ttgaaagtcc aagagttaga tataaatttg aataatacat gaannnnnnn
     2821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnagttcag aatatgaagc ctgatatagt
     2941 agaaaatttg aaagtctttg ttctccgctt ggtctgataa ttaaatctgg atctggaata
     3001 tcttttgtgt aaagatattc ctttatcaaa tcctcattta tattatttat atcaagattt
     3061 ttagaacata catcattgca tatgtttttt acagcatctg caatttcttg tcgtgcacca
     3121 taatttaatg caattatgaa attaagacca gatttatgct tcgatatctt ttcaatcttt
     3181 tccatactct taatcatact tttttttaat ttatctcttg atccgataaa acgtattctt
     3241 atgtttgatt tttccaatcg tcctgaagtg tcatttaaaa attcctgaag caaactcatt
     3301 aaataattaa cttcactttt aggtcttttc cagttttcag tagaaaaagt ataagctgta
     3361 atatatttaa tacccagctt atcactttct tcaattaaag tctgaattgc atctgcacca
     3421 catttatgac cataactgac aggcatcttt ttctgctttg cccaccttcc gttaccatcc
     3481 ataatgatgc caatatgttg aggtaattgc atttaaccta ttctccgttc ttgtgagatg
     3541 attaaacttc cattatctca tctgttttat tctttacgat atcatctatt tttgttatat
     3601 gctcatctgt tagtgtttga atatcttttt caataattgt ataatcatct tcagttattt
     3661 ctgagtcttt tctttgtttt ttgtattttt caatatcatc tcttctaata tttctgattt
     3721 ttaccttagc ttcttcacca tatgttttta cctttttaac aagttcttct cttctctctt
     3781 gagataagac tgggaatatt aagcgtataa ctcttccatc actttgtgga ttaataccaa
     3841 gatctgatgc ataaatcgct ttttctactt ctttaagaat agatgtatcc caaggttgaa
     3901 ttactaaaag tcttggttct ggtgaggaaa cattgcctat ttgatttagc ggggtcttct
     3961 gaccataata atcaacataa atattattaa gaacagaagg atttgctctt cctgctctta
     4021 ttgtggagat atgttcgctg aatgcatcaa tagcctgtga cattaattcg gttgttttac
     4081 tgtattcatc tttatttatc ataacaatac tccttttcgt ctagtaagat aaatacattc
     4141 tacacaatat gaggtcattt ttcaataatg gttcctatat ttccacctct agatgcttca
     4201 ataatatttt ttggttcatt taatgcaaac aatactgaac tcattgaaaa ttcatcacat
     4261 aatacagcag ctgccatatc tataactgta agttgctcag acaagatttt agaatatgtc
     4321 actctatcgt atttaattgc atccttatgt tttttaggat ccttatcata tacaccatca
     4381 acgtttttcg caaataatat tacatcagca tttatttcaa ttcctcttaa cgcagctgcc
     4441 atatcagttg agaagtaagg atttccagtt cctgcagcaa gtatagttat tattccattc
     4501 tttaaatctc tgactgcatc atctcttctg tatgttttgc atatctcgct cattaagact
     4561 gctgaatata cttcagaatc caaacctgtt ttttcaagag catctttaag tgcaatacta
     4621 ttaattatag ttgcaagcat acccatcgaa tcagctctgg atcgtacaat atctttaata
     4681 tctctacctc tgacgaaatt accagctcca actacaatag ctatttcctt accatcattt
     4741 ttaatctgtt taagcgcttt agcaatatcc attaaggttt ccatatcaat atttccacca
     4801 ttatttcctg agaacacctc accgcttaat tttattaata ttcttttata attcatatca
     4861 aactccaaaa taaaaggcac atttaagtgc cttgttattg ttatttactt atttgcttag
     4921 caacttcatc agcaaaattc tcttctctct tctgcaatcc ctcacccatc tcatatcttg
     4981 taaatcttct tacagaaata ttttcaccaa tctttgctat tatttcattg gtcatttgct
     5041 gtacagatat atctggattt cttatatatg cctgatctaa aagacaaact tgcttgtaaa
     5101 acttctcaat cctgccttca atcatttttt caacaacttt ttcgggttta ccctcgttta
     5161 aagcctgact ctttaaaata gctttttctt tttcaattat ttcagcagga atctcttctc
     5221 ttctgacata ctcaggtttt gatgctgcta tctgcattgc aatatcttta acatacgcta
     5281 aaaaatcttc atttttagct gcaaaatctg tttcaatatt tacttctaca agaacaccta
     5341 ttctgccacc gccgtgtata tacgatccaa caacaccttc ggctgctatt ctacctgatt
     5401 tcttttgagc attagctaat cctctttctc ttaatatatc tacagctttt gcaaaatcac
     5461 catttgattc aaccaaagcc ttcttacatt ccatcattcc gactccagtc ttttctctga
     5521 gttctttaac tgcacttgca ctaatcatta atattacctc ctatatacta ttacttaata
     5581 tcttcatcag aatcaacttc ggcatctgaa tcaacttcaa ccttggtctc aattcttgtt
     5641 tctatttctt tttcttcttt agctttatct tcaactaaat cattttcagc ttcaattttt
     5701 gctttttttg ctttttcttt cttagcttct tctactcttt tttcttcttt cttaatcttt
     5761 agaagttctt cttctgtaag cttagtcata tctatttctt catcatcctc aacagaaaaa
     5821 gattctcctt gattagcttc aataacagaa tttgctatta cacttgtaat aagttttaca
     5881 gctcttattg catcatcatt cccaggaatt acgtaatcaa cttcgtcagg atcgcagttt
     5941 gtatcaatta tagctacaac aggtatgtca agcttctttg cttcaagtac tgcgtttctt
     6001 tcttttttag gatcaattac aaaaagaaca tttggtatac catgcatatc ttttatgcca
     6061 ccaagattct tctctaattt ttccatttct gctttcaatt tgattacttc tttctttgga
     6121 agagattcaa aagtgccgtt attttccatt tcatgaagtt tcttaagata tgcaattctt
     6181 ttttcgatag tcttgtagtt agttaatgtt ccgccaagcc atcttgtatt gataaaaaac
     6241 attccacatc tcaaagcttc ttcttcaaca cattcctgag cttgtttctt tgttcctaca
     6301 aataatactt caccgccgtt aagaactatg ttttttacaa aatcataagc aagttctatc
     6361 tgttttactg ttttttgcaa atcaataata tgaattccgt ttctctcagt aaaaatatat
     6421 tctgacattt ttggattcca tcttcttgtt tggtgtccga aatgaacacc tgcttctaat
     6481 agttgtttca ttgaaataac tgacattttt attttcctcc gtttttttcc tctggcagct
     6541 tcatctttat gcacaacgtt ttacgcaact tacataaatt cact
//
LOCUS       DAOSXM010000107         6499 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2111
            NODE_7783_length_6499_cov_9.397424, whole genome shotgun sequence.
ACCESSION   DAOSXM010000107 DAOSXM010000000
VERSION     DAOSXM010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864178
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 6499)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6499)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:24:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,261
            CDSs (total)                      :: 2,229
            Genes (coding)                    :: 2,218
            CDSs (with protein)               :: 2,218
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 3 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6499
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7783_length_6499_cov_9.397424"
                     /isolate="AMR_MDS_2111"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            202..1209
                     /locus_tag="PLQ68_10090"
     CDS             202..1209
                     /locus_tag="PLQ68_10090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006058700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4065 domain-containing protein"
                     /protein_id="HXK73043.1"
                     /translation="MKRDKVFCEHCREDVEYSVLEAVQKGNLKGEEYEFIGKRTICNE
                     CSSDVFVAEIEDENLKSLYDSYRKINGIISLEKILEISQKYSIGKRPLSLLLGWGELT
                     FTRYCDGDMPTKQYSDILQRVYDDPEYFKKLLVENKERLKSAATFEKSMKKVLELLKE
                     NGETRTKFELIIKYLLYCCEDITPLALQKALYYVQGFYYAFMKAFLFDEDCEAWVHGP
                     VYRDIYKRYAEYHFDPIDKESDFDVSVFNDVEKAIIDSVVQYFCCYSGKMLESFTHSE
                     MPWLKTRDDLPANVNSNRIISKVDIGEYFSAVKEKYNMIRYNDIEEYAKSLFASCLKC
                     C"
     gene            complement(1385..3448)
                     /locus_tag="PLQ68_10095"
     CDS             complement(1385..3448)
                     /locus_tag="PLQ68_10095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK73044.1"
                     /translation="MNIIKKPWQSRYGWIWYNAKEIFEYTQKDMDDYVKQYADQGINI
                     LIGFSCTHFRFTFYNYWKEINACIEKIVKACHKYDILYVEHHSCHLTFNPKLKEDFEY
                     INNMFTKRYSKLSDFPKIILDTEKDFQIDGQNVSSFHQISGKTGTYGTTMYHGYAMCF
                     NNPDYKRIYLDYLKSLYEIGIDGIMTDDVQYFGNDNMGWSEFNACTCVHCRKLFKQKY
                     GYEIPLPSDWKSFYWNFENKVFVAWKRFKDESTLNFVYEVEEHYKNLGYNQFRPNYIS
                     SILPTNKTAYPFEKCSEVWDCVFQENCNSNIIKESYLCHGLEAIHRYHLARLNKVPSM
                     SMYYPSTQDNLYFSWALTKSWGQLYTACIYETKEGKMSEKHLRDFERRHIASYTNPKK
                     ITDLAFLLSSDTRDYSNGCETHQVNFLRWLEASYLSGISTDMIFEDAKMNEFKKHKVI
                     VCAHTVMLSDSVLNKLRKYIKEGGHLITIGEFAKYKPDGTIRKRIIIGRKNEALGDGN
                     ITVLSDNLCHNKYHWTLSVPRFIPDFDKIQKPVEEYAIDQLRETGGKAILSAIGNETI
                     VNATSNQDIFTSLFKIRNGYALHIVNVQDTISKEGTATHVQPIESFIENASRINHEIN
                     IRINLDIPVKKVTLYSPERKRALDIPYMKIKRISDDMNTSDTGLLSFTIPKKTFSGYA
                     LLEIK"
     gene            3825..4952
                     /locus_tag="PLQ68_10100"
     CDS             3825..4952
                     /locus_tag="PLQ68_10100"
                     /inference="COORDINATES: protein motif:HMM:TIGR02607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HXK73045.1"
                     /translation="MSKTITFNNTVAFHPGYYVKQMMDDMEINQNELAKRLDTTPKTV
                     SELINGNVNLSGDIAIKLAAMFGTSTELWLNLDKAYNEKKLEIDRRLKEEKECEIAKH
                     MDYNYWVKMNMVPETKKCVDKVRNMQKYFNIASLSLLDKPDFLVQYRSCKKEWDSKKL
                     INANAWVQTVINIGKDKKTSKYNEAKLKESIDDIRKMAKDPPDMIKRELEYLLASFGV
                     AFVVTPNLKNSNVTGAVKWVNDEKVIMGINDRMKYADVFWFSVFHELRHVMQRRLKSI
                     IVNYDIKESTEDPVLLKMEKDADEFAKDKLIPNKYYIKFINERVFNEYSIKSFASHID
                     SHPGIVVGRLQRDGHISYDKFNHMKIKYSFDFDNTEKESIF"
     gene            complement(4977..5513)
                     /locus_tag="PLQ68_10105"
     CDS             complement(4977..5513)
                     /locus_tag="PLQ68_10105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003455714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXK73046.1"
                     /translation="MKREKGTPLLEYLLPEGYRYVMYSPGDELSWAEIETSVGEFNKV
                     EDALEYYSKKYLPYIDELKRRCLFVENSKGKKIATLTAWWSYTDKRRDPWLQWFSVMP
                     EHQNKSIGKALVYECMKLLIEIEGDHDVYLPTQTWSYKAINIYRKQGFKIIPEKGLGG
                     FKNENYELAIELLKDYMR"
     gene            complement(5574..5978)
                     /locus_tag="PLQ68_10110"
     CDS             complement(5574..5978)
                     /locus_tag="PLQ68_10110"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HXK73047.1"
                     /translation="MKQVADKLNISISSLRYYDSQGLLPLIKRTAGNKRIFSDIDFEF
                     LKMIECLKITRMQLSDIRKYFYWCSLGDSTIRKRYELMLERKKEAEKQLKELQNSLEM
                     INYKCEYYRIANEAGTTNLPILNDLCKPDLHR"
     gene            5995..>6499
                     /locus_tag="PLQ68_10115"
     CDS             5995..>6499
                     /locus_tag="PLQ68_10115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK73048.1"
                     /translation="MKRAKIDKNFDISQAEAAYSRILGLKEPKKISIEQQKKRDKKQK
                     FSENLIFYIAGLIVLFILALTVPKAIIRKAVDLHICIAGEFNVKDHELLKQNLRPDVK
                     SRKTSVEVVYASFDTSDEISGYSTQVLSFTLLGTSHDLMICDRQVLYFLTNDPADIMV
                     LDMTEYLE"
BASE COUNT         2158 a          973 c         1148 g         2220 t
ORIGIN      
        1 aaatgaagtg ctatatgtat ttgttccaca gtataaatta ttcaacatag acggtattga
       61 aaaggtggtt gatgtttata caaaatttaa tttgattgaa tatcgcaata gtagaagaac
      121 agtggtgata tcatttcatg aaaggaataa acagataagt tattgcttca gatgatttac
      181 tattatcaag gaggaagaga tatgaaaaga gataaagttt tttgtgaaca ttgcagagaa
      241 gatgttgagt actcagtttt agaagctgta caaaaaggaa atttaaaagg tgaggaatat
      301 gagtttatag ggaagagaac tatatgcaat gaatgtagca gtgatgtttt tgtagctgaa
      361 attgaagatg agaaccttaa atcactttat gatagttata gaaaaatcaa tggtataatc
      421 tcattggaaa aaatactaga aatttcacag aaatatagta ttgggaaacg tcctttgtct
      481 ttgctgctag gatgggggga actgacattt accagatatt gtgatgggga tatgccaacg
      541 aaacagtatt ctgatatatt acagagagtt tatgatgatc cagaatattt caaaaaactg
      601 ctggtggaaa ataaggaaag attaaagtca gcagcaacct ttgaaaagag tatgaaaaag
      661 gttctggagt tgttgaaaga aaatggtgaa acacgaacaa agtttgaact tattattaaa
      721 tacctacttt actgttgtga ggatattacc ccgcttgcat tacaaaaggc tctttattat
      781 gtgcagggat tttattatgc ctttatgaag gcttttcttt ttgatgaaga ctgtgaagct
      841 tgggtacatg gacctgtata cagggatatt tacaaaagat atgcagagta tcattttgat
      901 ccaatagaca aagaatcaga ttttgatgta tcagttttca atgatgttga aaaggctatt
      961 attgacagtg tggttcagta tttttgttgc tatagtggaa aaatgcttga atcatttact
     1021 cattcagaaa tgccctggct aaaaactcga gatgatcttc ctgcaaatgt caattctaac
     1081 cgaataatca gtaaagttga tattggtgaa tatttttcag cagttaaaga gaaatataat
     1141 atgattagat acaacgatat tgaggaatat gcaaaaagtt tgtttgctag ttgtttaaag
     1201 tgctgctgag cttattgttt tggtaatgct tggtattcat tgttttgctt aggactaaga
     1261 ttaattttat gttacaaagt gaactaataa acagatattc ttatcctaaa gcccaaatat
     1321 gtaataagtt cacaagattg gtaaatcagc tagattaaat caaagtaatg taatccagtt
     1381 tgatttactt aatttccaat aatgcataac ctgaaaatgt ctttttgggt atggtaaatg
     1441 aaagtaaacc ggtatctgat gtattcatat catcagatat tctttttatc ttcatatatg
     1501 gtatatcaag agctcttttt ctttctggtg aataaagagt gacttttttt acaggtatat
     1561 caagatttat ccttatattt atctcatggt ttatacgtga agcattttca ataaagcttt
     1621 ctatcggttg aacatgagtt gctgtacctt ctttggatat tgtgtcctga acattgacta
     1681 tatgtaaagc atacccgttt ctaattttaa aaagggaagt aaagatatcc tgattgcttg
     1741 tggcatttac aattgtctca ttaccaattg cagataatat tgctttaccg ccagtttctc
     1801 ttagttgatc tattgcatat tcttcaacag gcttttgtat tttatcgaaa tcaggaatga
     1861 agcgaggcac acttagtgtc caatgatatt tattgtgaca tagattatct gataatacag
     1921 ttatattccc gtcaccaagt gcttcatttt tacggcctat tattattctc tttcttatcg
     1981 tgccgtctgg tttgtatttg gcaaactcac ctatggttat aaggtgccca ccttctttta
     2041 tatattttct aagtttattt agtactgaat ctgaaagcat tacagtgtga gcgcatacta
     2101 ttactttatg ttttttaaat tcattcattt tcgcatcttc aaatatcata tctgttgata
     2161 tgccggataa atatgaagct tccagccacc gtagaaaatt aacctggtgt gtttcacaac
     2221 catttgagta atcccttgtg tcagaggaaa gaaggaatgc taagtcggta atctttttag
     2281 gattcgtgta agatgcaata tgtcttcttt caaaatccct aagatgtttt tcactcattt
     2341 taccttcttt tgtttcatat atgcaggctg tataaagctg gccccatgat ttggtaagcg
     2401 cccaggaaaa atacaggtta tcctgtgttg aagggtaata cattgacatt gaaggtactt
     2461 tattcagtct tgctaagtgg tatctgtgaa ttgcctcaag accatggcac agatatgatt
     2521 ctttgattat attgctattg cagttttcct gaaatacaca gtcccatact tctgaacatt
     2581 tttcaaacgg gtatgcagtt ttgtttgttg gaagtataga ggatatgtag tttggacgaa
     2641 actgattata tccaaggttt ttataatgtt cttcaacttc atacacgaaa ttcagtgttg
     2701 attcatcttt aaatctcttc catgctacga aaaccttgtt ttcaaagttc cagtagaatg
     2761 atttccaatc agaaggaaga ggtatttcat atccatattt ctgtttgaac agtttcctgc
     2821 aatgaacgca tgtacaggca ttgaactcgc tccatcccat gttatcgttt ccaaaatact
     2881 gcacatcatc tgtcataatg ccatctatac ctatttcata caggcttttc agataatcaa
     2941 gatatattct tttgtagtct gggttattaa aacacatagc ataaccatgg tacatggttg
     3001 ttccgtatgt gccggtcttt ccagatatct gatgaaatga agatacattt tggccgtcta
     3061 tttgaaagtc tttttctgtg tctaatataa ttttaggaaa atctgagagt ttggagtatc
     3121 tttttgtgaa catgttgttt atgtattcaa aatcttcttt taatttaggg ttgaatgtaa
     3181 ggtgacagct gtgatgctct acatacaata tgtcatattt atggcatgct tttacaatct
     3241 tttcaataca cgcattaatt tctttccaat agttatagaa ggtgaaacga aaatgtgtac
     3301 aggagaatcc tataaggata tttatacctt gatcggcata ctgttttacg taatcatcca
     3361 tatctttctg ggtatactca aaaatttctt ttgcattata ccagatccat ccgtatctgc
     3421 tttgccaagg ttttttaatg atattcataa atacctcata tatttcattt atttgagtat
     3481 atcatgatta tatagaatga acaatgaata atacataccg aataattaat actatatatc
     3541 tgttacttgt gtaatatggt aataaggtgt ataatgtata aaatgttacc taaagggtaa
     3601 cgaaaagtga aaggataagg ttatagaagt aatatataag tcgaagcgag ttgaaaaggt
     3661 atgtacagat ttcaaaaaag caaggaaaga gttaggtaag gaaaacggtt ttaggctcat
     3721 aatcattcca cttaattgtg aacatgaaca atggactgaa aaagactttg ataaaatctg
     3781 catgaacact cagatagtag aaatacagga ggtatcaaaa cattatgagt aagacaataa
     3841 catttaacaa tacagttgca tttcatcctg gttattatgt aaaacagatg atggatgata
     3901 tggaaataaa tcagaatgaa cttgcaaaac gtcttgatac aacacctaag actgtatcag
     3961 aattaattaa tggtaatgtt aacttatctg gagatatagc aatcaagctt gccgcgatgt
     4021 ttggaacttc gacagaacta tggctaaatc ttgataaagc atataatgag aaaaagttag
     4081 aaatagacag aagactaaaa gaagaaaaag agtgtgagat cgctaaacat atggattaca
     4141 actattgggt taaaatgaac atggttcctg aaaccaaaaa atgtgttgat aaagtacgta
     4201 atatgcagaa atattttaat attgcttctt tatcgttgct ggataaacca gattttcttg
     4261 tccaatacag atcatgcaaa aaagaatggg acagcaaaaa actcataaat gccaacgcat
     4321 gggtacagac agttattaac ataggtaaag ataaaaaaac atcaaaatac aatgaagcaa
     4381 aactaaaaga gtcaattgat gatattagaa aaatggcaaa agaccctccg gatatgatta
     4441 aaagggagct agagtatctt ctggcatcat ttggagtagc atttgttgta actccaaatc
     4501 tgaaaaactc aaatgtgact ggagcagtaa agtgggtaaa cgatgaaaaa gttattatgg
     4561 gtattaatga cagaatgaaa tatgccgatg tgttttggtt ttctgttttt catgaattac
     4621 gtcatgtaat gcagagaaga cttaagtcca ttattgtcaa ttatgatatt aaagaatcaa
     4681 ctgaggatcc tgttttactc aaaatggaaa aggatgctga tgaatttgca aaagataaac
     4741 taataccaaa caaatactat attaagttca ttaatgaaag agtgtttaat gagtacagta
     4801 taaaaagctt tgcatcacat attgatagtc atccaggaat agttgtaggc aggttgcaaa
     4861 gagatggtca tatttcttat gacaagttca accatatgaa gattaagtac tcatttgatt
     4921 ttgataatac agaaaaagaa agtatttttt aatcaattta aattgcttat tttaacttat
     4981 ctcatatagt cttttaaaag ttcaattgca agctcatagt tttcattctt aaaaccgcca
     5041 agaccttttt cgggtattat cttaaaaccc tgttttctat atatgtttat agctttgtaa
     5101 ctccatgtct gagttggaag gtaaacatca tgatctcctt ctatttctat taacagcttc
     5161 atgcattcat atactaatgc ttttcctatt gatttgttct ggtgttcagg cattactgaa
     5221 aaccactgaa gccaagggtc tcgtctttta tctgtataac tccaccaggc tgttaaggta
     5281 gctatttttt ttccttttga attttctaca aacagacacc ttcttttaag ttcatcaata
     5341 taaggaagat atttcttaga atagtattcc aaagcatctt ctaccttatt aaattctcct
     5401 acagaggttt ctatctccgc ccatgacaat tcgtcaccag gactatacat aacatatctg
     5461 taaccttcag gtaaaaggta ttccaaaagg ggagtgcctt tttctctttt cataatgact
     5521 tcatagtact taatagtttt atccagcata ttttatgttt cctttcggct ttgttaacgg
     5581 tgcagatcag gtttgcataa gtcattcagt atgggaagat tagttgttcc agcttcatta
     5641 gctatacgat agtattcaca cttgtagtta atcatttcca atgaattttg caattccttt
     5701 aactgttttt ctgcttcttt ttttctttca agcataagct catatctttt ccttattgtg
     5761 gaatcaccaa gagaacacca gtaaaagtat tttcgtatat cactaagctg catccttgtg
     5821 atctttaaac attctatcat tttcaaaaac tcaaaatcaa tatcactgaa tattcgctta
     5881 tttcctgctg tcctttttat aagtggcagc agtccctgac tatcataata gcgaagtgat
     5941 gaaatagata tatttagctt atcagctact tgtttgattg agtattcctt gctcttgaaa
     6001 agagctaaaa tcgataaaaa ttttgatata tctcaagcag aagctgcata cagcagaata
     6061 ttaggattaa aagaaccaaa aaaaatatct atagagcaac agaagaaacg tgacaagaaa
     6121 cagaaatttt ctgaaaatct tatcttctac atagccggcc ttatagtctt attcatatta
     6181 gcgctgacag taccaaaagc tattataaga aaagcagttg atcttcatat atgtatagca
     6241 ggtgaattta atgtgaaaga ccacgagcta ttaaaacaga atctcagacc tgatgtaaaa
     6301 agcagaaaga catcagttga agttgtatat gcttctttcg acacatccga tgagattagt
     6361 ggatattcaa ctcaggttct gtcgtttact cttttaggaa cttcgcatga cttaatgata
     6421 tgtgaccgac aggttctgta ttttttgaca aatgaccctg ctgacataat ggttttggat
     6481 atgactgagt atctagaaa
//
LOCUS       DAOSXM010000108         6442 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2111
            NODE_7876_length_6442_cov_9.463911, whole genome shotgun sequence.
ACCESSION   DAOSXM010000108 DAOSXM010000000
VERSION     DAOSXM010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864178
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 6442)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6442)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:24:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,261
            CDSs (total)                      :: 2,229
            Genes (coding)                    :: 2,218
            CDSs (with protein)               :: 2,218
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 3 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6442
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7876_length_6442_cov_9.463911"
                     /isolate="AMR_MDS_2111"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1519)
                     /locus_tag="PLQ68_10120"
     CDS             complement(<1..1519)
                     /locus_tag="PLQ68_10120"
                     /inference="COORDINATES: protein motif:HMM:NF024637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HXK73049.1"
                     /translation="MYCTNCGKQIPDDSKFCTNCGANVSKPEMSQQPEQNFTAPRPFQ
                     PYSSIDPNQGIVNKTAFKGKQVSFGTSGSVSFNNGGKKGKKGLKIVLAVIAVLVVSVV
                     AVLIFSNMGKGSLKLGQTSALMNFTVPTSGTTVTVTDPSSDLAGMNISVPAGSYSSNV
                     DFNIETTDISSHSFGNNFNPITPLISVDNNEITSELPLRITIPIEKDPDEFAMAFYYD
                     RKTKKLEAIPFESLTDTSITILTTHFSEFVVSSVQTSSLASRSVDIDTGFMPGKDDWP
                     FVNYGSAIAPGGHCAGQSLTMAWYYHEKHIAAREPELNDRFDNNGDYLTEAFWIDDSY
                     GYRFASVIQDSINWTSPIFQNYVNVSYTNPEDTYYAFVYAMVMTKEPQFVGVYSHDEY
                     GNTTGGHALVAYKIDNGRLYVADPNYPGQKDRYIGVQNNMLLPYSSGANAAEIENYGG
                     TIYDHVTFVGKTALVDFNKIALNYNKVLNGTIGNEQLPTIYVQYFSRLDSVEGAIW"
     gene            1868..4969
                     /locus_tag="PLQ68_10125"
     CDS             1868..4969
                     /locus_tag="PLQ68_10125"
                     /inference="COORDINATES: protein motif:HMM:NF019552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HXK73050.1"
                     /translation="MENKITYETKFSKDEWNCVIPTPIIWNVEEQESLHINFKKSNWS
                     NYKILSIHIGSQTRTGCRVKMELLGPNDSIIASKFFAVDWEGSNNLKLWLDKLGFQPQ
                     KETFSEICGLRMICETPGIQKSVLSIERVSVYKQNPTWDVNKSDTIIDMSWASVYGEN
                     QWATNGANISEWNSVNNDSVEGFTRKQTNTGYLFEYTNNDANKQVLIVERKFDMDISD
                     FSQLLSKMIWSKDTLLTFTLIVDGNKLLNILENSKFPHDNVINTHLGDWLVIGGSLNK
                     AKHLEGIRITLTGNKKLEPISMKMFWILLRKSTEADYTPVKKVPVYLSSQNHKTRNDK
                     IVERQVKQMLLPSYDESVTPIKNPLTDGLPLGFYITRENLLQIRNSVLYGEGKTVLKR
                     IIEQADEAISSDLVQHNFYGTSYGGGVGLPKGVNGGGMKTYAPVVALAHLLTGEEKYA
                     INVRRWILRAARSEDWRGEHGGCVNRPQINDVLPFWDSFTGWYPKGYAGYMNHPFFIA
                     DVAYGVVISYDMIYHCLNVGERNEVENAFAKHGVYMLYDKLKNNRNFYINMNQGAIFA
                     TPLLMQTAFLKDKSETYREIYDWTLEFLKEYPLGTWSEEGVSREGPGYGMFSFSHYIE
                     AIFPLAACLNEKVENMVSDKMNNVFNYFLNCLSTCNEYEMYSMPISDLSGRWVWPGEL
                     LIFMAKYMKNTYAKYYWDKYYKDNPPPSIITLLSLDNDTLAEVPKLPLVQIYKDEPMA
                     FLRTGLSYGDTVLAMTNICNVKGHGHKDRASILLEYNKEPLILDPGYKNYSSPDVALY
                     SQTFCHNTMTFSQRNQKGGYNNYKTSITDYLSISESEHIIDWVTTDATDVYSEAVKYV
                     RHIIFLRPDIFIIVDDIIAKAFEKIELNFTCLGPVEKKDKNLFTSKTKMNQLNIHSYA
                     LEELEHNFKTFGTLWDDVPSYRLILSTKNMVKSCCFITALSPSPLDRENKIVIEKLNN
                     KDILGLSIKSNDCEDIFIYNINRNELIDYGVKTNCEMIILHKQKGILTGGIKFHNNKL
                     EKI"
     gene            complement(5054..5461)
                     /locus_tag="PLQ68_10130"
     CDS             complement(5054..5461)
                     /locus_tag="PLQ68_10130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009247572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HXK73051.1"
                     /translation="MKTKRIFILITFIIFTLVLTGCTVQNGDNVENTYKQISPKEAKQ
                     IMDDSDGYIILDVRTQSEYDQGHIPKAVLLPNETIGTDEIEILPDKNQLILVYCRSGN
                     RSKQASQKLVDLGYTNIYEFGGIIDWPYDIVTD"
     gene            complement(5533..5781)
                     /locus_tag="PLQ68_10135"
     CDS             complement(5533..5781)
                     /locus_tag="PLQ68_10135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK73052.1"
                     /translation="MRFNPFRIFDYIFRWNRLPDFSFNMSIPFLMIFAYLAVTVLSVL
                     GGVHMYKKTNNKIWVFLCGVPSILFIVIFALSRIIFFK"
     gene            6000..>6442
                     /locus_tag="PLQ68_10140"
     CDS             6000..>6442
                     /locus_tag="PLQ68_10140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK73053.1"
                     /translation="MKESIDNSSRTIPLVKSIRYTSYQFYARSEPAGGITSEELFKKE
                     ILYILNWVKKRCRYEDLPSPMIDIPAPENFKSYPIDKLQSFRHDIGYKLEVIITEDKK
                     TWAMQITEPDRGPYPDNPELNREVVPGRLFETDIGLKIKDKYVDIA"
BASE COUNT         2240 a         1064 c         1126 g         2012 t
ORIGIN      
        1 tccatatagc tccctctact gaatcaagcc ttgaaaaata ctgtacatat atggttggca
       61 gctgttcatt accgattgtt ccattaagta ctttattata gttaagagct atcttattaa
      121 agtcaactag agcggtctta cccacaaaag taacatggtc atatatcgtc ccgccataat
      181 tctcaatttc tgcagcgttg gctcctgaag aatatggaag caacatattg ttttgaacac
      241 ctatgtatct gtctttctgt cctggataat tcgggtcagc aacataaagc cttccgttat
      301 caattttgta tgctaccaaa gcatgccctc cagtggtatt gccatactca tcatgactgt
      361 acacgcctac gaactgaggt tctttcgtca ttaccatggc atatacaaaa gcataataag
      421 tatcttctgg atttgtatac gatacattga catagttttg gaaaatagga gatgtccagt
      481 taattgaatc ctgaattacc gatgcaaaac gatatccgta tgaatcatct atccagaaag
      541 cctctgttaa ataatctccg ttattatcaa acctgtcatt aagttcaggt tcccgagcag
      601 cgatatgttt ctcgtgataa taccatgcca ttgtaagaga ctggcccgca cagtgaccgc
      661 caggcgcaat tgcagagccg taatttacaa acggccagtc atcctttcct ggcataaagc
      721 ctgtatcaat atcaacagaa cgggatgcaa gtgaggaagt ctgaacactg gatactacaa
      781 attcagaaaa atgagtagtc agtatggtta tggatgtatc tgtaagactc tcaaatggga
      841 tagcttcaag ttttttggtt ttcctgtcgt agtaaaaagc catcgcgaac tcatctgggt
      901 ccttttcaat aggtattgtt attctaagcg gcagttcaga agtaatttcg ttgttatcaa
      961 cagatattaa aggagttatc ggattgaaat tattcccgaa actatgtgat gaaatatcag
     1021 tagtctcaat attgaaatca acatttgaac tgtatgaacc tgcaggtacc gatatattca
     1081 tacctgccaa atctgacgaa gggtcagtaa cagttactgt agtaccagat gttggaacag
     1141 taaagttcat cagcgcgctg gtctgcccaa gcttcagact ccccttaccc atgtttgaaa
     1201 agatcagtac tgcaacaaca gaaacaacta aaactgcaat tacagcaagt actatcttaa
     1261 gtcctttctt tcccttttta cctccattat taaaagaaac tgaaccagat gtaccaaaac
     1321 ttacctgttt acctttgaat gcagttttgt taactatacc ctggttagga tcaatagaac
     1381 tgtatggctg aaaaggtctt ggagcagtaa aattctgttc tggctgctga gacatttcag
     1441 gtttcgaaac atttgctcca caattcgtac agaatttgga atcatctggt atctgctttc
     1501 cacaattagt acaatacatg tctacccccg tattatgcat aaaatttata cttggttcac
     1561 aaaatgatta tagcggtttt gctaaattgc atttttcttt tctttagtat tcattcttgt
     1621 tctccaaagt acagtataaa tgtaccaaaa tatatttatg gtgtcaataa acattgaaaa
     1681 gtttgattat tttacattat taagataagt aatatctaat aactgttttt gcttctactg
     1741 tgcaggtttt tcttatgttt ttgaactttt acttatgacc caatcacaaa ttgacactgt
     1801 agtatactgt gtgttacgat tcaaaccata ttatgatggt aatcaattaa ttaaggatgt
     1861 tattattatg gaaaacaaaa taacatatga aacaaaattt tcgaaagatg aatggaactg
     1921 tgttattccc acccctatta tttggaatgt ggaagaacaa gaatcattac acataaactt
     1981 caaaaaatct aattggtcta attacaaaat actgtctatt catataggca gtcagacaag
     2041 aactggttgc agagttaaaa tggaattatt aggaccaaat gacagtatta ttgcaagtaa
     2101 atttttcgct gttgactggg aaggttcaaa taatcttaag ttatggctgg acaaacttgg
     2161 atttcagcca caaaaggaaa cattcagtga aatttgcggc cttcgcatga tatgtgaaac
     2221 tcccggtata caaaaaagtg tacttagtat agaaagagta agtgtatata agcagaaccc
     2281 gacatgggat gtaaataaaa gcgatacaat aatagatatg agctgggcaa gcgtttatgg
     2341 tgaaaatcaa tgggcaacaa atggtgcaaa cataagcgaa tggaattctg ttaataatga
     2401 ctctgtagaa ggatttacca gaaagcaaac caatacagga tatttatttg aatatacaaa
     2461 taacgatgcg aataaacaag ttttgatagt agaaagaaaa tttgatatgg atatatcaga
     2521 cttttcccaa ctactgtcta aaatgatctg gtctaaagac acactgctta ctttcacact
     2581 aatagtagat ggaaataaat tgttaaatat acttgaaaat agtaaattcc cacacgacaa
     2641 tgtcatcaat acccatttag gagactggtt agttattgga ggcagtttaa ataaagccaa
     2701 acatttagaa ggtatacgca ttaccttaac tggcaataaa aaactagaac caatcagcat
     2761 gaaaatgttc tggattctgc ttcgcaaaag cactgaagct gattatactc cggttaaaaa
     2821 agtccctgta tatctgtctt cacaaaatca taaaacaaga aatgataaaa ttgttgaaag
     2881 acaagtcaaa cagatgctac ttccgtctta tgatgagtca gtaacaccta ttaaaaatcc
     2941 tttaacagat gggttacccc ttggattcta tataacaaga gagaatttgt tacagataag
     3001 aaactccgtt ttatatggag aaggaaaaac agtacttaaa agaatcattg aacaggctga
     3061 tgaggcgata agttcagatt tagtgcagca taatttttat ggcacatcat atggtggagg
     3121 tgtaggattg ccaaaaggcg taaatggcgg aggaatgaaa acctatgcgc ctgtagtcgc
     3181 attggctcat ttgcttacag gcgaggaaaa atatgctata aatgtcagaa ggtggatatt
     3241 aagagcagca agaagtgaag attggagagg tgaacatggc ggttgcgtga acagaccgca
     3301 aattaatgat gtattacctt tctgggacag ttttactggt tggtatccta aaggttatgc
     3361 agggtatatg aaccatcctt tttttattgc tgacgtcgct tatggtgttg taatttccta
     3421 tgatatgata tatcactgtt taaatgttgg agaacgaaat gaagtggaaa atgcatttgc
     3481 aaaacatggc gtgtatatgc tttatgacaa attaaagaat aaccgtaatt tttatatcaa
     3541 catgaatcag ggagccatat ttgcaacacc acttttaatg caaactgcct ttttaaagga
     3601 taaaagtgaa acatatagag aaatatatga ctggacttta gaatttctaa aggagtatcc
     3661 tttaggaacc tggagtgaag aaggcgtttc aagagaaggt cctgggtatg gaatgttttc
     3721 tttttcacac tatattgaag ctatatttcc cttagccgca tgtttaaatg aaaaggttga
     3781 aaatatggtt tctgataaaa tgaataatgt tttcaactac tttttaaatt gtcttagcac
     3841 ttgtaatgaa tatgaaatgt attctatgcc cattagcgac ttaagcgggc gatgggtatg
     3901 gccaggcgaa ttactgattt tcatggcaaa atatatgaaa aatacatatg ctaaatatta
     3961 ctgggataaa tactataaag ataatcctcc tccaagcata ataacgcttt tatcattaga
     4021 taatgatacg ttagctgaag tgccaaagtt accgcttgtt cagatttaca aagatgagcc
     4081 tatggcattt ttgcgaacag gtttatcata tggcgataca gttttagcaa tgacgaatat
     4141 atgcaatgta aaaggtcatg gacataaaga ccgggcaagc attcttcttg agtataataa
     4201 agagccatta atacttgatc caggatataa aaactatagt tcacctgatg ttgcattgta
     4261 ttctcagaca ttttgccaca atacaatgac attttcacaa agaaatcaaa aaggtggata
     4321 taataattac aaaacatcaa taactgacta tttatccata tctgaaagtg aacacataat
     4381 tgactgggtt actaccgatg caactgatgt atatagtgaa gcagtaaaat atgtaagaca
     4441 cataattttt ctaagacctg atatttttat aattgttgat gacatcatag caaaggcttt
     4501 tgaaaaaatt gaacttaatt ttacatgttt gggtccagtt gaaaaaaaag acaaaaatct
     4561 ttttacttca aaaacaaaaa tgaatcagct taatatacat tcatatgcat tagaagaact
     4621 tgaacataat tttaaaactt ttggcacatt atgggatgat gtaccttcat acagactgat
     4681 actaagcaca aaaaatatgg ttaaatcctg ctgttttata acagcactat cgccatcacc
     4741 tttggataga gaaaataaaa ttgttataga aaaactaaat aacaaggaca tattgggttt
     4801 aagcataaag tcgaatgatt gtgaggatat tttcatatat aatatcaata gaaatgaact
     4861 tatagattat ggtgttaaaa cgaattgtga aatgattata cttcacaaac agaaaggtat
     4921 tttaactggt ggaataaagt ttcataacaa taagctagaa aagatctgat cattcaactg
     4981 gatgtttctt tgtaatgttg catataatct taactattga gtaatataac ttcttgtatt
     5041 tttaatatac ttatcagtct gttactatgt catagggcca gtctattata ccgccaaatt
     5101 catatatatt ggtatatcca agatctacaa gcttctgtga tgcctgcttg cttctgtttc
     5161 cagatcggca gtatacaagt attagctggt ttttatctgg caatatttct atttcatcag
     5221 ttcctatggt ttcattaggc agtaatactg cctttggtat atgaccctgg tcatactcag
     5281 attgagtcct tacatcaagt atgatgtatc catctgagtc atccataatc tgttttgcct
     5341 ctttgggcga tatctgctta tatgtatttt ccacattatc tccattttgg actgtacatc
     5401 cagttagtac taaagtaaaa ataataaatg ttataagtat aaagattctt tttgttttca
     5461 tgtcacacct caattaagtc tttgtataga atacacgctt taaacaaata catctgtgac
     5521 aaaagcacat tattacttaa aaaatataat cctgcttaat gcaaatatga ctatgaaaag
     5581 tatacttggt actccacaga gaaataccca tatcttgtta ttggtttttt tgtacatatg
     5641 tactccacct agtacagaca atactgtaac ggctaaatat gcaaatatca taagaaaagg
     5701 aatactcatg ttaaaagaga aatctggcaa ccggttccat cggaaaatat aatcgaatat
     5761 tctgaaagga ttgaatctca tgcaaatcac ctcctggata tgataccaca tatacttata
     5821 gaaaatcatt aaaaattcct taaaaactat gacttgctta aactgaaaaa taaagttttc
     5881 actttgtgca tatttaaatg tgtataatat atatgtaaaa ccgaaatact tgattaacaa
     5941 atctgcaatt tgtgccttat gcataattta tattatttta tttattcaaa ggggaaatta
     6001 tgaaagaatc tattgataac agcagcagaa ccataccgtt agtcaaaagc atacgttaca
     6061 catcatatca gttttatgcc agatcagaac ctgcaggcgg tataacatct gaagaattat
     6121 ttaaaaagga aatactctat attttaaact gggttaaaaa gagatgccga tatgaggatc
     6181 ttccatcacc tatgattgac ataccagcac ctgaaaattt taaatcatac ccgatagata
     6241 aactgcagtc atttcgtcat gatataggtt ataaattaga agtaattata actgaggata
     6301 aaaaaacatg ggcaatgcag ataacagaac ctgacagagg cccctaccct gacaaccctg
     6361 aactaaacag agaagttgta cctggacgtt tgtttgaaac agatatagga ttaaaaatta
     6421 aagacaaata tgtggacatt gc
//
LOCUS       DAOSXM010000109         6386 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2111
            NODE_7976_length_6386_cov_9.628495, whole genome shotgun sequence.
ACCESSION   DAOSXM010000109 DAOSXM010000000
VERSION     DAOSXM010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864178
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 6386)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6386)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:24:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,261
            CDSs (total)                      :: 2,229
            Genes (coding)                    :: 2,218
            CDSs (with protein)               :: 2,218
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 3 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6386
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7976_length_6386_cov_9.628495"
                     /isolate="AMR_MDS_2111"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(22..1146)
                     /locus_tag="PLQ68_10145"
     CDS             complement(22..1146)
                     /locus_tag="PLQ68_10145"
                     /inference="COORDINATES: protein motif:HMM:NF015666.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase N-terminal
                     domain-containing protein"
                     /protein_id="HXK73054.1"
                     /translation="MNRAVIIGAGKTGRGFIARLLAQQGIEIVFVDIDEVLINKLNIS
                     SKYCVHFFNNEAKKVEIENFKAYTWQNADISDIEHIFISVGAGNLNDVGERLCKVLNK
                     KKQYYIIGCENSTSTAKILNNSINMKNVTISEAAVFCTTVEDEKLDILSEDYPNLEVN
                     AKLLKGYNLKIPSIKYIDDFSVLMKRKLYTYNAASSIIAYLGWLKGYSVYSQAANDRE
                     ISKILDMFYIHINQSICKEYGCDIALQEEFASLSKQKFCDKSIYDTVERNARNPLKKL
                     MSDERIIMPMILMQKHSFDCTISQLTAAAAMVYAKFTSDASKSLNGGLGDLYENILID
                     ICKLKNNTDNYVKIYDKAILLEEKLNRGYEICLYDFLVVK"
     gene            complement(1139..2311)
                     /locus_tag="PLQ68_10150"
     CDS             complement(1139..2311)
                     /locus_tag="PLQ68_10150"
                     /inference="COORDINATES: protein motif:HMM:NF019736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK73055.1"
                     /translation="MTCVIFGAGKIARGFIAHLLYLSGIRFVFVEKNQALVELINQKK
                     RYCINVMGNSDKNICIDNVSALGYIQENEIVELILDSDAIFTAVGGKNLIDTASIIAK
                     GICKKIDKKEKLNIITCENWKQPALCLKNEILKYLSDNYLKEFDLYIGITEAVVMRSA
                     IEPDKNSLIEDPLLVNVQNFWELPVNASDMVSGFPNILGVRAISSFEGFLEKKFYTYN
                     AANATVSYLGALLGYKVLSKAAKDERVIKVLDGVYSETSSALCKKYGFSNEEQKEFVK
                     GSKMKLQDETITDYIERNARDPIRKLSCDDRLIGPARMAIEYDILPCNLCIAIAAAIY
                     YENAEDESSNTLKKMREIQGVDTVLDRVCKIDTNGILGKLIKCKIELLKLKGWINE"
     gene            complement(2308..3690)
                     /locus_tag="PLQ68_10155"
     CDS             complement(2308..3690)
                     /locus_tag="PLQ68_10155"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HXK73056.1"
                     /translation="MKKYVLGIDIGTSSVKAICKSIESEVNVAIESYESCSVFGWWTA
                     FKKVLLNFDLKNVIALGLTGQIGTYIINKEDVIHWSDSYGKEQLDEIMDKFNKEQFLK
                     EIQMNHPRLISFPIPRLMYISSKYNFLESIMMPKEYIVQKLTGNCISDNCSWRGLYNF
                     KEKKYSNLFMDYIGIKEEILPKIVNMEEKGGFVTRSASVETGLTEGVPVFIGCNDFYS
                     ALIGMGITKENQVFDLTGTSEHAGVIVKGMNSENALVCSPYLDKFVHYGVTASSGASL
                     NFNKINFGEKDIDIHSCITKNPPIFLPYLNGERAPVWDCNASGVFFGLTDKTDKELLS
                     YSVFEGVVFSIFSILKTIPSFKPVTVTVGGGAANNEVLNKLKAEIFSSDVLLLEQNQS
                     SAFGAYMIAAVGIGIYTSLDEAVKINCIKKKIIKHDGQFSNLFMQRYEIYESMYPLLK
                     DTFGKFRGIK"
     gene            complement(3687..4700)
                     /locus_tag="PLQ68_10160"
     CDS             complement(3687..4700)
                     /locus_tag="PLQ68_10160"
                     /inference="COORDINATES: protein motif:HMM:NF028222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HXK73057.1"
                     /translation="MKAIVVEKANKVILKEVPIPNPDKGYARIKVSACAICATDLELI
                     GGNIPANYPIIPGHEWSGVVDAVSDDSYSDWLEKPVTGSNDISCLTCDECKRGNIRYC
                     KSFEEIGFRRNGAYAEYLTVPVHNLVEIPEGISFEEAALSEPLAVAIGVLEKAQARLG
                     ETLLIFGAGSIGLCILAVAKAMGLNKITVCASSQNRLDIAKKMGAFRTIASNDTNIIE
                     EMKKIHPDGTDIVCDATGMEECIVNGIKLTRKGGRLLLAGYSRGKSMNIRIDDIHVNN
                     IKVIGAGNNWNVHKKAVALMESHAVDISMLITERIQLHQYKYGLELARTRPFGFVKAV
                     FVL"
     gene            complement(4715..5800)
                     /locus_tag="PLQ68_10165"
     CDS             complement(4715..5800)
                     /locus_tag="PLQ68_10165"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HXK73058.1"
                     /translation="MKYINHKSMKRANSTDIFFLIKNNKQLTRKQIEELTGLSWGAVS
                     NITGELVNKGYVIEKKQPSKSKAGRIPKELMINPNDFFFIGLDINISGLKAVLVNLEN
                     DIKATWNEMPDYTDKDTFLKSILVFIDKIMTGYAKSHILGMGIAMQGEIDTNKGMSIN
                     VKQCKGWENVPLLNIISDRFQMPVFIEHDPDCILYAEHIEKDLSSALLIRLDRSIGMA
                     VMSQGAMIRGKGMLEIGKMVVSSDLNLEDVVTQNGIERAYMKPFKQIADEAKSNVFYA
                     CKIFKEMNKHLSIAIINMSILFNVKKVILCGNMMEYKDLFCEELSDAIKKGLNGNLEE
                     IAITDVKNAAYGAALIAIEKSAYVLDL"
     gene            5861..>6386
                     /locus_tag="PLQ68_10170"
     CDS             5861..>6386
                     /locus_tag="PLQ68_10170"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXK73059.1"
                     /translation="MKNKITNTRLSNDQIGIFYIGQAGFLFKYNNIYVLIDPYLSDYV
                     DRYCSTEKIKWKRKYAPPVEPQELSFVDYVICTHAHLDHMDPDTISKIYQNRTNSKMK
                     FIIPAPALNDLLNIGVNEDDIIKAYADQTINIDGISVTALPAAHETINRDIYGNCIEL
                     GFIFRFGNKTVYHSG"
BASE COUNT         1975 a         1305 c          825 g         2281 t
ORIGIN      
        1 attgcatttg cggcctattc gctattttac cacaagaaaa tcataaaggc aaatttcata
       61 tcctctattt aatttttctt caagcaaaat ggctttatca tatattttta cgtagttatc
      121 tgtattattt tttagtttac atatatctat aagtatattt tcatataaat cacctaaacc
      181 accattcaga cttttactag catctgaagt gaattttgca tataccatcg ctgcagctgc
      241 agttaactgg gaaattgtac agtcaaatga atgcttttgc atgagaatca taggcataat
      301 aattctttca tctgacatca gttttttcaa aggatttcta gcatttctct caactgtgtc
      361 atatatagac ttatcgcaga atttttgttt ggacagactt gcgaattctt cctgcaaagc
      421 aatatcacat ccatattctt tacatataga ttggttgatg tgtatataga acatatccag
      481 aattttactg atttctctat catttgcagc ttgcgaatat acactatacc cctttaacca
      541 gcccaaatat gcaataatac tgcttgcagc gttatatgta tagagttttc ttttcataag
      601 aacactaaaa tcatctatat acttaataga aggaattttt agattatatc cttttagtaa
      661 tttcgcattt acctcaagat ttggataatc ttctgacaaa atatccagtt tctcatcttc
      721 tacagtggta cagaaaaccg ctgcttcaga aatagttaca tttttcatat taatagagtt
      781 atttaatatt tttgcagtcg aggtggaatt ttcacacccg ataatataat actgcttttt
      841 tttattaagt actttacaaa gcctttctcc tacatcattc aaattacctg ctccgacaga
      901 tataaatata tgctcaatat ctgatatgtc tgcattctgc catgtgtaag ctttgaagtt
      961 ttctatttca actttttttg cttcgttatt aaaaaaatgc acacagtatt tagaactaat
     1021 attaagttta ttaattaata cctcatctat atccacaaaa actatttcaa tcccctgttg
     1081 agctaatagt cttgcaatga atcctctgcc tgtcttacct gcaccaatta taactgctct
     1141 attcatttat ccatcctttt agtttcaaca gttctatttt acacttaatc agtttgccaa
     1201 gaattccatt agtatcaatt ttgcatactc tgtctagcac tgtgtctaca ccttgaattt
     1261 ctctcatttt cttaagtgta tttgatgatt catcttctgc attttcataa tatatggcag
     1321 cagcaatagc tatacacaaa ttacatggca gtatatcata ttcaatagcc attcttgcag
     1381 gaccaatcag tctgtcatca caacttagtt ttcttatggg gtctctggca tttctttcta
     1441 tgtaatcagt tattgtttca tcctgaagtt tcattttaga acctttgaca aattccttct
     1501 gttcttcatt tgaaaaccca tattttttac ataatgctga tgatgtctca gaatatactc
     1561 catcaagcac ttttataacc ctctcatcct ttgctgcttt cgataaaacc ttgtatccta
     1621 acaaagctcc caaatatgaa acagttgcat tagctgcatt atatgtataa aacttctttt
     1681 ccaaaaaacc ttcaaatgat gaaattgctc taactcctaa aatgtttgga aatccagata
     1741 ccatatcaga tgcgttaact ggtaattccc agaagttttg aacattcact aaaagaggat
     1801 cttcaatgag actattctta tccggttcaa tggcggagcg cattacaaca gcttcagtta
     1861 tcccaatata taaatcaaat tccttcaaat aattatcaga aagatatttg agtatttcat
     1921 ttttaagaca taacgcaggc tgtttccagt tctcacatgt aataatgttc agcttttctt
     1981 ttttatcaat ttttttacat attcccttag caattattga agctgtatct attaaattct
     2041 tacctccgac agccgtaaat atagcatcac tatccaagat taattcaaca atttcatttt
     2101 cctggatata accaagagca gatacattat caatacatat atttttatcg gaattaccca
     2161 taacatttat acagtatctt ttcttttgat taatcaattc taccaatgct tgattttttt
     2221 caacaaagac aaatcttatc ccacttaaat aaagaagatg agcaataaac cctctggcaa
     2281 ttttccccgc tccgaatatt acacaggtca tttaatacct ctgaactttc cgaatgtatc
     2341 cttaagcaac ggatacatac tttcatatat ctcatatctt tgcataaaaa gattactaaa
     2401 ctgcccatca tgtttaatta tcttcttttt aatacaatta atcttcaccg cctcatcaag
     2461 acttgtataa atacctatcc cgactgcagc tatcatatat gcaccaaaag cagaactctg
     2521 attctgttca agtaataata catccgatga aaatatttca gctttcagtt tgttcagaac
     2581 ttcattattt gccgcacctc ctccaacagt tacagtaaca ggcttaaatg aaggaattgt
     2641 ttttaatata ctgaatatac tgaacaccac accctcaaat acagaataag acaatagttc
     2701 tttatcagtt ttgtcagtta acccaaaaaa tacaccagat gcattacagt cccatacagg
     2761 tgctcgttcg ccatttagat aaggaagaaa tatcggagga ttttttgtta tacacgaatg
     2821 gatatcaatg tctttttcac caaaattaat tttattgaaa ttaagtgaag caccagaaga
     2881 agctgttact ccataatgaa caaatttatc aagatacgga ctgcatacaa gagcattctc
     2941 actattcatg ccttttacaa ttacacctgc atgctcactg gttccagtta aatcaaacac
     3001 ctgattttct tttgtgattc ccatacctat aagcgctgag taaaaatcat tacatccaat
     3061 aaaaactggt acaccttctg tcagtcctgt ttcaactgaa gctgaacgcg ttacaaatcc
     3121 gcctttttcc tccatgttaa caattttagg caggatttct tcttttatac ctatgtagtc
     3181 cataaaaagg ttgctgtatt ttttttcttt aaaattatat aaaccacgcc atgaacaatt
     3241 atctgatata cagttaccag taagtttttg aactatgtac tcttttggca tcattatact
     3301 ttcaagaaag ttgtatttac ttgagatata cataagtctg ggtattggaa aggatattag
     3361 tcttgggtga ttcatttgta tctcttttaa gaactgctct ttattaaatt tatccataat
     3421 ttcatcaagc tgttctttgc catacgaatc actccagtgt ataacatctt ctttgttgat
     3481 tatatatgta ccaatctgac ctgtcagtcc taaagcaatt acatttttta aatcaaaatt
     3541 gagtagcacc tttttaaaag cagtccacca tccaaaaacc gaacatgatt cgtatgattc
     3601 aattgcaaca ttaacttctg attcaatgct tttacatata gcttttaccg atgaagttcc
     3661 aatatctatt cctaatacat atttcttcat aacacaaata ccgcctttac aaaaccaaaa
     3721 ggtcttgttc ttgcaagttc cagtccgtat ttatactgat gcaactgaat tctttcggtt
     3781 ataagcatgg aaatatctac tgcatgcgat tccataagag caactgcttt tttatgaaca
     3841 ttccagttat tacccgctcc tatgactttt atattattaa catgaatatc atcaattcta
     3901 atgttcatgc tttttcctct tgaatatcct gcaagtaaca atcttccacc ttttcttgta
     3961 agcttaatac cattaactat acattcttcc atacctgttg catcgcagac aatatccgtt
     4021 ccatctggat gtattttttt catttcttca attatattcg tatcatttga tgctatagtc
     4081 ctgaatgctc ccattttttt agcaatatcc agtctgttct gagacgaagc gcaaactgtt
     4141 atcttattca gacccattgc ttttgcaact gccagtatgc aaagaccgat agaacctgca
     4201 ccgaatatca ataaagtttc tccaagtctt gcctgtgctt tttcaagaac gccgatagcc
     4261 actgctaaag gttcgcttaa tgcagcttct tcaaaagaga taccctctgg tatctcaacc
     4321 agattgtgaa caggtactgt taaatattcc gcatatgctc catttcttct aaacccaatc
     4381 tcctcaaagc ttttacagta tcttatatta cctcttttac attcatcaca tgtcagacaa
     4441 cttatatcat tactacccgt aacaggtttt tcgagccagt cactgtatga atcatctgaa
     4501 actgcatcaa ctacaccact ccattcatgg ccgggaatta tggggtaatt cgcaggtata
     4561 ttaccgccaa ttagctctaa atcagttgca catatcgcac aagctgaaac ctttattctt
     4621 gcataaccct tatcagggtt tggaataggt acttctttaa gtattacttt attcgctttt
     4681 tccactacaa ttgctttcac attatcacct tttcctataa atccaataca taagctgatt
     4741 tttcaattgc tataagagct gcaccatatg ctgcattttt cacatcggtt attgctatct
     4801 cttctaagtt tccgtttaat ccttttttta ttgcatcact aagttcttca cagaataaat
     4861 ccttgtactc catcatattt ccgcataata ttactttttt aacattgaac aaaatactca
     4921 tgttaattat agctatacta aggtgcttgt tcatttcttt aaaaatcttg caagcgtaaa
     4981 aaacatttga ctttgcctca tcagcaatct gtttaaatgg cttcatatat gctctctcta
     5041 taccattctg tgttactaca tcctccagat ttaaatcaga actgactacc attttcccga
     5101 tttccagcat acctttaccc cttatcattg caccttgaga catcactgcc attccaatgc
     5161 ttctgtcaag ccttataagc aaagcactgc ttaaatcttt ttcaatatgt tctgcataca
     5221 gtatgcagtc tggatcatgt tcaataaata ccggcatttg aaatctgtca ctgataatgt
     5281 taagaagcgg cacattctcc catcccttac actgcttaac atttatagac atgcctttat
     5341 tggtatctat ttcaccctgc atagctatcc ccatacctag aatatggctt tttgcatatc
     5401 cagtcataat tttatctata aaaactagta ttgattttaa aaatgtatct ttatctgtat
     5461 aatcaggcat ttcattccat gtagccttaa tatcattttc taaattaaca agaacagctt
     5521 ttaagccaga tatgttaata tccaatccaa taaaaaagaa gtcattcgga ttaatcatta
     5581 gttcttttgg tatccttcct gctttacttt tcgagggctg tttcttttct attacatacc
     5641 ctttattcac aagttcaccg gtaatattgg acaccgctcc ccagctcaaa ccagtcagct
     5701 cttctatttg tttacgagta agctgcttat tattcttaat taagaaaaat atatctgtgc
     5761 tgttagctct cttcatgctt ttgtgattaa tatatttcat ttcatctccg catgttgtaa
     5821 ttatatcatt aagtgatata attacaatag gtacaataat atgaaaaaca agattacaaa
     5881 tacaagatta tcaaatgacc aaataggcat attctatata gggcaagcag ggtttttatt
     5941 caagtataac aacatatatg tacttattga tccttatctt tccgattatg tagacagata
     6001 ttgcagtact gaaaaaatca aatggaaaag aaagtatgca ccgcctgtag aaccccaaga
     6061 attaagtttt gttgattatg tcatctgtac acatgcacac cttgaccata tggatccgga
     6121 taccatatca aaaatatatc aaaacagaac aaatagcaaa atgaagttca taattcctgc
     6181 tcctgcttta aatgatttat tgaatatagg ggtaaatgag gatgatatta taaaagccta
     6241 tgctgaccag acaatcaata ttgacggtat atccgtaact gctctgcctg cagcgcatga
     6301 aacgataaat agggatattt acggtaactg cattgagctt ggatttatat ttcgttttgg
     6361 aaataaaacg gtataccata gcggag
//
LOCUS       DAOSXM010000110         6281 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2111
            NODE_8163_length_6281_cov_11.569708, whole genome shotgun sequence.
ACCESSION   DAOSXM010000110 DAOSXM010000000
VERSION     DAOSXM010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864178
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 6281)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6281)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:24:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,261
            CDSs (total)                      :: 2,229
            Genes (coding)                    :: 2,218
            CDSs (with protein)               :: 2,218
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 3 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6281
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8163_length_6281_cov_11.569708"
                     /isolate="AMR_MDS_2111"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..425)
                     /locus_tag="PLQ68_10175"
     CDS             complement(<1..425)
                     /locus_tag="PLQ68_10175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HXK73060.1"
                     /translation="MKNILKRYWIFLLLLIVNVVIGLIFPEIGKRSLEISKQNVIEML
                     LVIPPIFVLLGLLDVWVDRTTMVKFMGKGSGIKGVLIAFLLGSAAAGPLYAAFPVAGV
                     MMKKGSSLFNIFIFIGAWSTTKIPMLTFEAASLGLTFTLL"
     gene            complement(425..901)
                     /locus_tag="PLQ68_10180"
     CDS             complement(425..901)
                     /locus_tag="PLQ68_10180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019226719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HXK73061.1"
                     /translation="MFTYIIYGLAVIGLSVSFFKDRQKTKMALTKAWKSFENILPQFL
                     AILVIIGIIFAILSPSTIQVLLGSNSGFLGVIGASLIGAITLIPGFVAFPLAAALLNS
                     GAGYMQIAAFVASLMMVGIITLPLEIKTFGKKAAVIRNVSAYAFSLIAALIIGVIL"
     gene            complement(909..1259)
                     /locus_tag="PLQ68_10185"
     CDS             complement(909..1259)
                     /locus_tag="PLQ68_10185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013485758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HXK73062.1"
                     /translation="MGLFDKFKKKEEKEECCSCCNCNSESVEKASKEINEDASVKILG
                     SGCSKCNELEKATKEALDMLNMNDTIDHVTDFAKIAAYGVISTPALVVDEKVVSYGKV
                     LKTDEVLKILKKIR"
     gene            complement(1313..2002)
                     /locus_tag="PLQ68_10190"
     CDS             complement(1313..2002)
                     /locus_tag="PLQ68_10190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015042236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HXK73063.1"
                     /translation="MTELLDKIAPLLTDIGWLTPLLALIAGVLTSFMPCSLSSIPLVV
                     GYVGGTAQNDSKKAFKLSIIYVLGTAVTFTAIGVIAALAGKLVGVTGSWWYIILGVLM
                     VLMALQTWGIFEFIPSSNLLSKSTKKGGIGAFIAGMLAGVFSSPCATPVLVALIAIVA
                     TQNSILWGAFLFILYSIGHGVLSVVAGTSVGFVQKLASSEKYGKASKILSIVMGLIIL
                     VIGLYMFYLGF"
     gene            complement(1999..>2340)
                     /locus_tag="PLQ68_10195"
     CDS             complement(1999..>2340)
                     /locus_tag="PLQ68_10195"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HXK73064.1"
                     /translation="PIVLDFGSDSCLPCISFHPTLKNMHKDMYGRVIIKYIDVYKYGE
                     AVQGFPVSVLPTQIFINADGTPYVPSKNVNIPFAVYNYNTTGEHAYTAHEGVLTKTQF
                     LEILEDMGVSQ"
     assembly_gap    2341..2440
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2441..3130)
                     /locus_tag="PLQ68_10200"
     CDS             complement(<2441..3130)
                     /locus_tag="PLQ68_10200"
                     /inference="COORDINATES: protein motif:HMM:NF022321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2703 domain-containing protein"
                     /protein_id="HXK73065.1"
                     /translation="MAKKWYPVIDYSECLECGNCIRKCTHSVYDMKKAPVPVVLMPDN
                     CIDRCHGCGNICPVGAIEYVGDDTGWTPKAKQTNFVEKSTCSCGSLKKVIIEYLYLDQ
                     EVCDRCIDTEEILKEAIDNVSEELEKKGFEVIYRKTQIENQLMATKFRFVSSPTIRIN
                     GYDIFSTVYENECGCCSSIASESVKCRAYEYEGEVYDVPTVEMVSESILKQIESCCDI
                     KRVENKYVIPEN"
     gene            complement(3139..4152)
                     /locus_tag="PLQ68_10205"
     CDS             complement(3139..4152)
                     /locus_tag="PLQ68_10205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007049875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HXK73066.1"
                     /translation="METLKAIGLFVQNEILGMKWLDRLVAAMLEGFGMDLDTWYAASI
                     RFFIYDVIKILILLSFLIFIISYIQSYFPPERTKRILGRFKGIGANILAALLGTVTPF
                     CSCSSIPLFIGFTSAGLPVGVTFSFLISSPLVDLGSLVLLTSIFGFKVAITYVIVGLV
                     LAVIGGTIIEKTGMDKYVEQFVKNANSVQIEDQEMKIKDRLKYAYEQLAATVKKVAPY
                     ILVGVLIGALIHNVIPEIWVQTVLGSNNPFSPVLAAVIGIPMYADIFGTIPIAEALFA
                     KGVGIGTILSFMMGVTALSLPSLIMLKKAVKTKLLVVFTVIVSVGIILIGFLFNALQG
                     LFI"
     gene            4294..5208
                     /locus_tag="PLQ68_10210"
     CDS             4294..5208
                     /locus_tag="PLQ68_10210"
                     /inference="COORDINATES: protein motif:HMM:NF013335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HXK73067.1"
                     /translation="MKNIYEPIKSKRRNIILDTDIGPDCDDAAAIAILFEMQKEHNFH
                     ISGIVNSSSNPYGTKAIETLCKYYEYNDIPIGSYSKPDGLGDQIKYNKYLANNILPTL
                     KGKDNPKMDSLKLYKEALESSEDDGTVIITIGSFLAISEALRAYPELFEKKVYAVISM
                     AGRFSERKTEFNVEWNISAAKYFFENFKNTIICSGFEVGVDVITGFEDMIMPDLTNPV
                     KMSYYLFTDGLNTRPSWDLTAVEFAVNGQSDIFCLSEPGVINITKEGFTEYTAKHNGK
                     HMYIIKNTSSNEIAIYLNNLLKRTTDKK"
     gene            complement(5222..>6281)
                     /locus_tag="PLQ68_10215"
     CDS             complement(5222..>6281)
                     /locus_tag="PLQ68_10215"
                     /inference="COORDINATES: protein motif:HMM:NF022623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HXK73068.1"
                     /translation="IIEFSMKEGKYMFTELTKKSSFKAMRESSYDIFGGNLDLRSIKP
                     GETLVMADLEGPGKIVHWWCTIAAEDIHMFNRMLVLRFYWDDEKNPSVETPIGDFFCS
                     GHGMESEVISLPITSLNRGKGRNCWFEMPFKKRALLTITNDAEEPVRSFYWNIDWQKF
                     DSVNDDILYFHAQYKQEYPTKEGKDYRILEAFGAGNYVGTVLNVEKDIKPGWFGEGDE
                     KIYIDGENYPSIWGTGTEDYFLTAWSPHVYQSPFSGFSIWQGCNHAGDKTTGYRFHIL
                     DPIPFTKSIVVDIEHAWTVENKEFCDFYSSVAFWYQSEPHVPFDNIPDANQRRAQRPE
                     EIKYDKHYQDKWGMKAGE"
BASE COUNT         2038 a         1259 c          898 g         1986 t
ORIGIN      
        1 agcaatgtaa aagttaatcc cagactggct gcttcaaaag tcagcatagg tatttttgtt
       61 gttgaccatg ctccaatgaa tataaatata ttaaaaagac tgcttccttt cttcatcatt
      121 actccagcaa ctggaaaagc tgcgtataag ggtccagctg cagctgaacc taataaaaaa
      181 gctattagga ctcctttgat tccagaccct tttcccataa atttaaccat agtagttcta
      241 tctacccaaa catcgagtaa tcctaacaga acaaagatag gcggtataac aagcagcatc
      301 tcaattacat tctgttttga aatctctaat gaccttttac ctatttcagg aaatataagt
      361 ccaataacta cattaacaat cagtaataat aagaatatcc aatatctttt aagtatattt
      421 ttaatcataa tatcacccct attattaacg cagcaataag agaaaaagca tatgctgaaa
      481 catttcttat aacagcagct ttcttaccaa atgtttttat ttccaatggc agggtaatta
      541 taccaaccat cattaaagat gctacaaaag ccgcaatctg catataccct gctccactgt
      601 tcaataaagc tgctgcaagc gggaaagcta caaatccagg tatcaaagtt attgctccga
      661 taagagatgc gcctataact cccaaaaaac cggaattact gcctaaaagc acctgtatag
      721 tacttggact cagtattgca aaaataatac ctattataac caatattgct agaaactgtg
      781 gaagtatatt ctcaaaagac ttccatgctt tcgttaaagc cattttcgtt ttttgtctat
      841 ccttaaaaaa cgatactgat aatccaatta cagctaaacc gtaaatgata tatgtaaaca
      901 tatgtatcct accttatctt ctttagtatt tttaaaactt catctgtttt aagaactttg
      961 ccatacgata caaccttttc atcaacaacc agtgcaggag tactgatgac tccgtaagct
     1021 gcaatttttg caaagtctgt aacatggtca atagtatcat tcatatttag catatcaaga
     1081 gcttctttag ttgctttttc cagttcatta cacttgctac aaccgcttcc aagtattttc
     1141 actgaagcat cttcattaat ttctttactt gctttttcta ctgactctga attgcagtta
     1201 cagcagctgc aacattcttc cttctcttct tttttcttaa acttatcaaa tagtcccata
     1261 cttatttgta tgatacagtt ataacctgct catacttctc cttttatatt atttaaaaac
     1321 ccagatagaa catatataac cctattacta atatgatcaa tcccattact atgcttaata
     1381 ttttgcttgc ttttccatat ttttcgctag aggcaagctt ttgaacaaaa cctactgaag
     1441 ttcctgcaac aaccgaaagc actccatggc ctattgaata tagtataaac aggaaagctc
     1501 cccataatat actgttctgt gttgcaacta tggctataag cgctactaaa accggagttg
     1561 cgcatggtga ggaaaatact ccagctagca ttccagcaat aaatgcgcct attcctcctt
     1621 ttttcgtact ttttgacagc aaattacttg atggtatgaa ttcaaaaatt ccccatgtct
     1681 gcaaagccat taaaaccata agaacaccta agataatata ccaccatgaa cctgtaacgc
     1741 ctacaagttt gcctgcaagt gctgcaatca ctcctattgc tgtaaatgtt acagcagtgc
     1801 ctaaaacata gattattgaa agcttaaatg ctttttttga atcattttgc gctgtacctc
     1861 ctacatatcc cacaaccaat ggtattgaag ataaagaaca tggcataaaa gaggttaaaa
     1921 cacctgcaat caatgcaagc aaaggggtaa gccagcctat atcagtaagt aatggtgcaa
     1981 ttttatcaag taattctgtc attggctcac tcccatatct tcaagtattt ctaaaaactg
     2041 agtcttagtc agtacgcctt catgcgcagt gtatgcatgt tcacctgttg tgttgtagtt
     2101 atatacagca aaaggaatat ttacattctt acttggcaca tatggtgttc catctgcatt
     2161 tataaaaatc tgtgtaggta atactgaaac tggaaaaccc tgcacagctt ctccatattt
     2221 atatacgtca atatacttga ttattactct gccatacata tctttatgca tatttttcaa
     2281 ggtcggatga aaacttatac agggaagaca ggaatcagaa ccgaaatcca gcactatcgg
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn attttctggt ataacatatt
     2461 tattctcaac cctttttata tcacaacagg attcaatttg tttaagtata ctttcagata
     2521 ccatttcaac agtcgggaca tcatatactt caccttcata ctcatatgct ctgcatttca
     2581 cagattcaga tgcaatactt gaacagcatc cacactcatt ttcataaact gttgaaaaaa
     2641 tatcatatcc gtttatgcgt atagtaggcg aacttacaaa cctaaactta gttgccatca
     2701 gctgattttc tatctgagtt tttctataaa tgacttcaaa tccttttttt tctaattctt
     2761 cagatacatt atctatagct tcttttaaaa tctcttctgt atcaatgcac ctgtcacata
     2821 cttcctggtc cagataaaga tattcaataa ttactttttt caatgagccg catgaacatg
     2881 tacttttctc tacaaaattt gtctgttttg cttttggagt ccaccctgtg tcatcaccta
     2941 catattcgat tgcgccaact ggacaaatat tcccacatcc atgacatctg tcaatacagt
     3001 tgtcaggcat gagtacaacc ggtactggag cctttttcat gtcataaact gaatgtgtac
     3061 actttctaat gcaatttcca cattccaaac attctgaata atcaattacc ggataccact
     3121 tttttgccat ttttcctcct aaataaataa gccttgaaga gcattaaaca agaaacctat
     3181 taaaattata cctactgata caatcaccgt aaatacaact aacagttttg tctttactgc
     3241 ttttttcagc atgatgagag aaggtaatga taatgcagta acacccatca taaatgaaag
     3301 tattgtacca attcctactc cttttgcaaa caaagcttca gctataggta tagtaccgaa
     3361 aatatcagca tacataggta tgcctatgac tgcagctaaa acaggagaaa acggattatt
     3421 tgatccaaga actgtctgta cccatatttc tggaattaca ttatgaataa gagctccgat
     3481 aagtactccg actaatatat atggagctac tttcttaact gttgctgcta actgctcata
     3541 tgcatatttg agcctgtctt ttatcttcat ttcctgatct tcaatctgta ctgagttagc
     3601 attttttaca aactgttcaa catatttgtc cattcctgtt ttttcaataa tggtccctcc
     3661 gattactgca agaacaagac ctacgataac ataagttatt gcgactttaa atccaaatat
     3721 gcttgtaagt aataccaaag aacctaaatc aactaaaggt gaagatatta aaaatgaaaa
     3781 cgttactcct acaggtaacc cggcagatgt gaaaccaatg aacaaaggta ttgatgaaca
     3841 ggagcaaaaa ggcgttactg ttcctaaaag agctgcaagt atattcgcac caataccttt
     3901 gaatctaccc aatattcttt tcgttctttc aggtgggaag taactttgaa tatatgaaat
     3961 tatgaaaatc aggaaggata aaagtataag aatttttata acatcataaa taaaaaatct
     4021 tatactcgca gcataccacg tatctaaatc cataccaaat ccttcaagca tagctgcaac
     4081 taatctgtcc agccatttca ttccaagtat ctcattctgt acaaaaagcc ctatcgcttt
     4141 taaagtttcc ataaaacctc acattgacaa tcatcaatgt gataatttta cacattacat
     4201 tgatatatgt caatatgtta tttcataaat attcctcaga atcacctcat ctatgataaa
     4261 atattaatat attaaaaaaa taggaagcat attatgaaaa acatctatga gccaatcaaa
     4321 tcaaagagaa gaaatatcat tttagatact gatataggac ctgactgtga tgatgcagca
     4381 gcaattgcta tactatttga aatgcagaaa gaacataatt ttcatatatc aggtattgta
     4441 aacagttctt ctaaccctta tggcactaaa gcaatagaaa ctttgtgtaa gtattatgaa
     4501 tataatgata taccaatagg ttcatacagc aaacctgacg gacttggaga tcagataaaa
     4561 tataataaat atcttgctaa caatattctt ccaactctta aaggaaaaga taatccaaaa
     4621 atggattctt taaagttata caaagaggct cttgaaagtt ctgaagatga cggtacagtt
     4681 attataacaa taggttcttt tttagcaata agcgaagcat tgagagctta tcccgaactt
     4741 ttcgaaaaaa aggtttacgc agtaatttcc atggctggtc gctttagtga aagaaaaaca
     4801 gagtttaatg tcgaatggaa catttcagca gcaaaatatt ttttcgagaa ctttaaaaac
     4861 accattatat gctctggatt tgaagtagga gttgatgtta taaccggatt tgaagatatg
     4921 attatgcctg accttacaaa ccctgtcaag atgtcatatt acctgtttac cgatggactt
     4981 aatacaagac cttcatggga tctgacggct gtagaatttg cagttaacgg ccagtcggat
     5041 atattctgtt tatccgaacc aggagttatt aatataacaa aagaaggttt tacagaatat
     5101 actgccaaac ataatgggaa acatatgtat attattaaga acacttccag caatgagatc
     5161 gctatttatc taaataacct gctaaaaaga actactgata agaaataggc taaatgtaaa
     5221 gctattcgcc agctttcatt ccccatttat cctgatagtg cttatcatat ttaatttcct
     5281 caggcctttg cgctcttctt tggtttgcat ccgggatatt gtcaaatgga acatgaggct
     5341 cagattgata ccaaaaagcc accgatgaat aaaaatcgca gaactccttg ttttcaactg
     5401 tccaggcatg ttcaatatct actactatgc tttttgtaaa aggaatagga tcaagtatat
     5461 gaaacctgta accggttgtt ttatctcctg catgattgca accctgccag attgaaaatc
     5521 ctgaaaatgg tgattgatat acatgcgggc tccatgctgt gagaaaatag tcttctgtac
     5581 cagtacccca tatggaagga taattttctc cgtctatata tattttctca tctccttcac
     5641 caaaccatcc tggctttata tctttttcga cattcaatac cgtacctaca taattgcctg
     5701 ctccaaaagc ttctaaaatc ctgtaatcct taccttcttt agtcgggtat tcctgtttgt
     5761 attgagcatg aaaatacagt atatcatcat ttactgaatc aaatttctgc cagtcaatat
     5821 tccagtaaaa agacctgacc ggttcttcag cgtcatttgt tatggtcaac agagcacgtt
     5881 ttttaaatgg catttcaaac cagcagtttc tcccctttcc tctgttcaaa gaagttatag
     5941 gcaaagaaat cacttcactt tccataccat gtcctgaaca gaagaaatcc ccaattggag
     6001 tttcaacact tggatttttc tcatcatccc agtaaaatct cagtacaagc atcctgttaa
     6061 acatatggat atcttccgct gctatagtac accaccagtg tacaatttta cccggacctt
     6121 caaggtctgc cataactagt gtctcacctg gcttaatact ccttagatca aggtttccac
     6181 cgaagatgtc ataacttgat tctctcatag ctttaaatga agatttcttt gtaagctctg
     6241 taaacatata ttttccttct ttcattgaaa attcaattat a
//
LOCUS       DAOSXM010000111         6216 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2111
            NODE_8284_length_6216_cov_5.849375, whole genome shotgun sequence.
ACCESSION   DAOSXM010000111 DAOSXM010000000
VERSION     DAOSXM010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864178
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 6216)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6216)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:24:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,261
            CDSs (total)                      :: 2,229
            Genes (coding)                    :: 2,218
            CDSs (with protein)               :: 2,218
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 3 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6216
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8284_length_6216_cov_5.849375"
                     /isolate="AMR_MDS_2111"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..215
                     /locus_tag="PLQ68_10220"
     CDS             <1..215
                     /locus_tag="PLQ68_10220"
                     /inference="COORDINATES: protein motif:HMM:NF014669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015225 - biotin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015878 - biotin transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HXK73069.1"
                     /translation="IKNRVAAIALCIAGLITCHLAGVIQFMIVSNTAFIPTVAAISLP
                     YMIKDIASCVIAYLVYMQLKKVITVE"
     gene            complement(246..1340)
                     /locus_tag="PLQ68_10225"
     CDS             complement(246..1340)
                     /locus_tag="PLQ68_10225"
                     /inference="COORDINATES: protein motif:HMM:NF024702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HXK73070.1"
                     /translation="MDKGKKKRKRRYLILLLAVLLAVSALIIYVCIDKSNNPFIVQLS
                     DGGMVITGYQGFSREIDIPDEIDSVYVIAIGDRAFADNKRITSISMPDSVTSIGEGVF
                     SNCSSLEQIKLSLNIEEICNNTFLNCKSLEKVVLPDGVETVGAMAFCGCTALNDIKLS
                     ANLVSVGEYTFSECQSLESINLPEGLLSICNGAFSRCYVLESITIPDDIQTICEDTFS
                     ECTSLKDVELSDSVKTIEKNAFLNCKSLEYIDLSSVESVGNSAFMYCFGLDEISLSES
                     MVIIEERAFYCCKSLKELVLPDIGIVIKPGAFGNCISLSRIIMPPNVNLQKSDAFYGC
                     PNVVIYGKEGSFAETYAKERKIPFVSYIFK"
     gene            complement(1365..2675)
                     /locus_tag="PLQ68_10230"
     CDS             complement(1365..2675)
                     /locus_tag="PLQ68_10230"
                     /inference="COORDINATES: protein motif:HMM:NF024702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat protein"
                     /protein_id="HXK73071.1"
                     /translation="MKDLSIRRQYCRVRLYIIYMMLMSITICSCSAPLLVNATRPISK
                     LIGDYLYGIYDNNILYGYIGKDKDIVIPEINDNLIEIRSLGTNEYVRNVTLNDKVWRV
                     SGGAFFRCKNLENIYVDPDNEYFSSIDGVLFDKEGTTLISYPLSRKQKEYHIPEGTLN
                     VSLITTPININYLNFKAHLKRIYLPASIREFNTEAFYLFERLIEIYVDSKNEKYISID
                     GMLIEKETGELVLYPEAKSKRFISPDEVKSIGEKVFYQKGIRHAEFNDNLSKISTCAF
                     FGCRLRKVEIPDNVSIIEEDAFDSNRIRTLKLGDSVRIIDKSAFSRNRISKLNLGNSV
                     EIIGSIAFEGNRITSLHLNDSLQWIGGGAFAGNNIRGELIFPDSLEYIGKDAFTSEKI
                     SKVVISDGVFIEGSLFGSDEFKKAYDEYGAGTYLRIEEGKWVKE"
     gene            complement(2910..4292)
                     /locus_tag="PLQ68_10235"
     CDS             complement(2910..4292)
                     /locus_tag="PLQ68_10235"
                     /inference="COORDINATES: protein motif:HMM:NF024702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat protein"
                     /protein_id="HXK73072.1"
                     /translation="MMKNLSSKKQYCRVRLCIIYMMLMSIVLCMFIQPLVVNAYRPSV
                     KRQIGDYIYRQYDLSFIQGYVGVDEDIEIPILKDGFITIESLGDNKYVKSITLNEKVW
                     NVLSNALDGCKNLKNIFVDPDNEHLCSIDGVLFDKEGTTLLIYPPGRTQTEYHVPEGT
                     FYVSSEAFRVERSFYDFRGYLKRIYLPESMKEFIANELYALDTQKAVYVDSRNEKYMS
                     IDGMLINKEIGELILYPGAKSKRFISPDEVKSIGEKVFYQKGIRYARFNDNLSKISAY
                     AFWGCRLRKVDIPDNVSIIEADAFDDNKIRTLKLGDSVRIIDVSAFSRNRIRKLNLGS
                     SVEVIGTIAFQDNKITRLKLNDSLEWIGGGAFADNRITRLKLNDSLEYIGEGAFSGNN
                     IRGELIFPDSLEYIGEDAFTSEKISKVVISDGVFVEGSLFGSDEFKKAYDEHGAGTYM
                     RTDEGEWVKQ"
     gene            complement(4335..4712)
                     /locus_tag="PLQ68_10240"
     CDS             complement(4335..4712)
                     /locus_tag="PLQ68_10240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK73073.1"
                     /translation="MKKITKILLVVMVIFLVSITSIVNANSVGDKELCINGKVYVDLN
                     TDGAFTEDELNRNGIVIELTELVNTTTDKEGTTIEEYRTVETSVVNENGEYNFKINPK
                     NKYKIILNRNERKNYIYDDIESC"
     gene            complement(4755..6002)
                     /locus_tag="PLQ68_10245"
     CDS             complement(4755..6002)
                     /locus_tag="PLQ68_10245"
                     /inference="COORDINATES: protein motif:HMM:NF024702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HXK73074.1"
                     /translation="MKDLSSRKQYGRIRFYIIYMILMSIVLCMCIQPLGVSALSPLPT
                     YKQGDYLYNYLHHSIVGYVGKEKDIVTPVINDDCFNVSFLGENKNIRSVTLNAKVNKV
                     GEGAFSMCKNLVNIYVDPDNEYFTSIDGVLFDKEGTTLIAYPQNKGQKEYQIPEGTKY
                     ISSTAFTMKRGFFDFRTLLHKIYLPDSLEEYTGLTFFKCITLKEIHIKDTNEKYTVEN
                     GIVFDKDMKEIVYYPKIKSSYYVVPEGIRTIGYYAFFNSGLRYIRLNEGLEEISEGAF
                     AHCRLRKVIIPDTVKLIERNAFGGNKIHTLKLGKSVEKIDGNAFQSNNITKLELNDTL
                     TYIGGHAFAWNDIKGTLIFPKSLECIGPNAFTSKEVSRVIIWDGIRVLGSLFGSEEFK
                     EAYDKYGAGAYIVTDESKWIKER"
BASE COUNT         1855 a         1333 c          841 g         2187 t
ORIGIN      
        1 atattaagaa tagagtagca gcaatagcct tatgtatagc aggattgatt acctgccatt
       61 tagccggagt aatacagttt atgatagtat caaacacagc atttatacct acagtagctg
      121 caatatctct tccttacatg attaaggata tagcatcatg cgtaatagct tatcttgtat
      181 atatgcagtt aaagaaggtt ataactgtag aatagtcata taacccacta ctcttgaatg
      241 cttaatcatt taaaaatgta actcacaaac ggtattttcc gttccttagc atatgtttca
      301 gcaaatgaac cttccttacc atatataact acattaggac aaccatagaa tgcgtcagat
      361 ttttgaagat tcacattagg cggcattatt atcctgctaa gacttataca attaccaaaa
      421 gcaccaggtt ttattacaat accaatgtcc ggtaaaacaa gttctttaag gcttttacag
      481 caatagaacg ccctctcttc gataattacc atactttcag ataaagatat ttcatcaaga
      541 ccaaagcagt acatgaaagc tgagttgcct acgctctcca cagagcttaa gtcaatatat
      601 tccagacttt tacagttaag aaatgcattt ttctcaatcg tttttactga atctgacagt
      661 tcaacatctt tcaagctagt acattcacta aaagtgtctt cacaaatcgt ttgtatgtca
      721 tcaggaatcg taatgctttc aagcacatag catcttgaaa atgctccgtt gcatatggac
      781 agcaaacctt caggtaagtt gattgattct agagattggc attcactaaa tgtgtattcc
      841 ccaacagata ctagattcgc tgacaacttt atatcattta gtgctgtaca gccgcaaaaa
      901 gccattgcgc ctactgtttc cacaccatca ggtaatacaa ccttctcaag gcttttgcaa
      961 tttaaaaagg tgttgttaca tatttcttca atattcaatg acaactttat ttgttccaat
     1021 gaagaacagt tggaaaatac accttctcct attgaagtta cactgtcagg catacttata
     1081 cttgttatac gcttattatc agcaaaagct ctatcaccga tggcaataac ataaaccgag
     1141 tctatctcat caggtatgtc aatctccctg gaaaatccct gatatcctgt tattaccatg
     1201 cctccatcag ataattgcac aatgaatgga ttgttgcttt tatcaataca gacatatatt
     1261 attaatgctg atacagccag aagaactgct aacagaagaa ttaaatatct tctttttctt
     1321 ttctttttac ctttatccat atctgtttac ctcgacacaa attctcattc ttttacccat
     1381 ttaccttctt caatccgcaa atatgtccca gctccatatt catcatatgc tttcttgaat
     1441 tcgtcagacc cgaacaagct tccttctata aataccccgt ctgatattac cactttagat
     1501 atcttttcag atgtgaatgc gtcttttcct atatactcca agctgtcagg aaatatcagc
     1561 tctcctctaa tattgtttcc tgcaaatgca cctccaccaa tccattgcaa tgaatcattt
     1621 agatgcaggc tggttatcct gttgccttca aatgctatgc ttcctattat ttccacactg
     1681 tttcccaagt tgagtttact tattctattc ctgctaaatg cacttttatc aattatcctg
     1741 actgaatcac ccagcttaag agtccttatc ctgttactat caaaagcatc ttcttctata
     1801 atcgaaacat tgtcaggaat ttcaacctta cgtaatctac atccaaaaaa tgcgcatgtg
     1861 ctgattttag acagattatc attaaactct gcatgccgga ttcctttctg ataaaaaacc
     1921 ttctccccta tactcttcac ttcgtcaggg gaaatgaagc gtttagattt tgcttcagga
     1981 tagagtacca gctctcctgt ctccttctcg ataagcatcc cgtcaatgct gatgtacttt
     2041 tcgttcttac tgtccacata tatttctatt agtctctcga atagataaaa tgcttctgta
     2101 ttgaattctc ttatacttgc tggtaaatat atccttttaa gatgcgcttt aaaattcaaa
     2161 tagtttatat ttattggagt ggttataagt gatacattta aagttccttc aggtatgtgg
     2221 tattcttttt gttttctact taaaggatac gatataaggg ttgtaccctc cttgtcaaac
     2281 agaacaccgt ctatggaact gaaatactca ttgtcaggat ctacgtaaat attttccaaa
     2341 ttcttgcaac ggaaaaaagc ccctccactt actctccaaa ccttgtcatt caatgttaca
     2401 ttccttacat attcattagt tcctaatgag cgaatttcaa ttaaattatc atttatttca
     2461 gggattacta tatccttatc cttccctata taaccataca agatattgtt atcataaatt
     2521 ccatataaat aatctccgat tagtttcgaa atcgggcgtg ttgcattcac aagtaaagga
     2581 gctgaacatg agcatattgt aatactcatt aacatcatat atatgatata aagcctgact
     2641 ctgcaatatt gccttctgat tgataagtct ttcatcttaa tattactcct tgtaaacagc
     2701 tttatctata tatagtcata ctcaacagct ccgcatcttg agcatgtcct cttatattca
     2761 tacacataac tctgccagtc ctcgtcataa tatgtgtact ggtataccca cccgctccag
     2821 ttgtgcccaa gtgccggtat agcctgtgtt gacagtacag agctgtatcc gtagcagatc
     2881 tgctttaagt atacaatata ttatgtaatc tactgcttta cccattcccc ttcatcagtc
     2941 cgcatatatg tgcctgcacc gtgctcatcg tatgccttct tgaattcatc agatccgaac
     3001 aggcttcctt caacaaatac cccgtctgat attactactt ttgatatctt ttcagatgtg
     3061 aatgcgtctt cccctatata ctccaagctg tcaggaaata tcagctcgcc tctaatattg
     3121 ttaccagaaa atgcaccttc accaatatat tccaatgaat cattgagttt aagtcttgtt
     3181 atcctgttgt ctgcaaatgc acctcctcca atccattcca atgaatcatt gagtttaagt
     3241 cttgttatct tgttgtcttg aaaagctatc gtccctatta cttccacact gcttcctaag
     3301 ttgagtttcc ttattcgatt ccgactaaat gcacttacat caattatcct gactgaatcg
     3361 ccaagcttaa gagtccttat tttgttatcg tcaaaagcgt cagcttcaat aatcgaaaca
     3421 ttgtcaggta tatcgacctt tcgtaatctg caaccccaaa aagcatatgc gctgatttta
     3481 gacagattat cgttgaatct agcatatcga attccttttt ggtaaaaaac cttctcccct
     3541 atactcttta cttcgtcagg ggaaatgaag cgcttagatt ttgctccagg atagagaatc
     3601 aactccccta tctccttgtt tataagcatc ccatcaatgc tcatatactt ctcgttcctg
     3661 ctgtccacat aaacagcttt ttgagtatca agtgcataaa gttcatttgc aataaattcc
     3721 ttcatgcttt cgggaagata aatcctctta aggtaccctc tgaaatcata aaaacttctt
     3781 tcaacacgaa atgcctcact tgatacataa aaagtgcctt caggtacatg atattccgtt
     3841 tgcgttctgc ctggtggata tattaaaagg gtcgtaccct ccttgtcaaa caggactcca
     3901 tcaatagaac ataaatgctc attatcaggg tctacgaata tgttcttcaa atttttacag
     3961 ccatctaatg cattacttag tacattccat actttctcat tcaatgttat actctttaca
     4021 tatttgttat cacccaatga ctcaatagta ataaatccat cttttaaaat agggatctct
     4081 atatcttcat ccactcctac ataaccttgt atgaaactaa gatcatattg acgatatata
     4141 tagtctccaa tttgacgttt tacactcggc ctgtaagcat tcacaaccaa aggctgtata
     4201 aacatacaca acactatact cattaacatc atatatatga tgcaaagcct gactctgcaa
     4261 tattgttttt ttgaagataa gtttttcatc ataatgtgtc tcctaaaaaa gtctttatct
     4321 aaatatagtc atactcaaca gctttcgata tcatcatata tgtaattttt gcgttcattt
     4381 ctgttcagaa taatcttata tttattcttt ggattaatct tgaaattata ctctccattt
     4441 tcattgacca cacttgtttc aacagtccta tattcctcaa tagttgtacc ttctttatca
     4501 gtagttgtat taactaattc ggtaagctca ataacaatcc catttctatt taactcatcc
     4561 tcagtaaatg caccatcagt gttcaaatcg acatatactt taccattaat acataattcc
     4621 ttatctccaa cagaatttgc attaactatt gaagttatcg acacaaggaa tataaccata
     4681 actactaaca aaatctttgt tatctttttc atattttttt cctttctttt tcattctatc
     4741 actaatatat ttcatcatct ttcttttatc catttacttt catcagtcac tatatatgca
     4801 cctgccccat acttgtcata tgcttcttta aattcttccg aaccaaaaag agaacctaat
     4861 acgcgtatcc catcccatat tatgactcta gatacttctt ttgatgtgaa agcatttggg
     4921 ccgatacatt ctaaactctt aggaaatata agtgttcctt ttatgtcatt ccatgcgaat
     4981 gcatgcccgc ctatgtaggt aagtgtatca ttcagctcta gcttagtgat attgttactc
     5041 tgaaaagcat taccgtctat tttttcaact gattttccta attttagtgt atggatcttg
     5101 ttccctccaa aagcatttcg ttctattaat ttgaccgtgt caggtatgat aaccttccgt
     5161 agtcggcaat gtgcaaatgc tccttcgctg atttcttcta gcccttcatt cagtcttata
     5221 tatctcagac cactgttgaa gaaagcataa taacctattg tcctgatccc ttcgggcacg
     5281 acatagtagc ttgatttaat cttcggataa tatactattt ctttcatatc cttatcaaaa
     5341 acaataccat tttctactgt atatttttca ttagtgtctt tgatatgtat ttcttttaaa
     5401 gttatgcatt tgaaaaaagt taatccggta tattcctcca aactgtccgg aagatatatc
     5461 ttatgcagaa gagtcctgaa gtcgaaaaat cctcttttca tggtgaaagc tgttgaggat
     5521 atgtatttcg ttccttccgg tatttgatat tccttctgtc ctttattttg cggataagct
     5581 ataagggtag taccttcttt gtcaaataaa acaccatcaa tagaggtgaa gtattcatta
     5641 tccggatcaa catatatgtt cactagattt ttgcacatac taaacgcacc ttcaccgact
     5701 ttgtttactt tcgcatttaa agttacactc cttatatttt tattctcacc caagaatgaa
     5761 acattaaagc aatcgtcatt aatgacagga gttacaatat ccttctcttt acctacatag
     5821 ccaacgatac tgtggtgtaa ataattgtat aaataatctc cttgtttata tgttggtaac
     5881 ggactgagcg cactgacacc cagaggctgt atacacatac acaacactat actcattagt
     5941 atcatatata tgatataaaa cctgattctg ccatattgtt ttctggaaga taagtctttc
     6001 atcttaatat gtctcctata taaatcagct gtttacttgg tattaatatt atatctgtaa
     6061 cattatcata taatatcttc cataatttta caatattaac agtgaattga gtttccgtgt
     6121 tttatcttgt atataaataa aactgtaatt aatctgtcaa aaacctaatg aaaatggcct
     6181 taaataagga aatgcctctc gatttttggt ataata
//
LOCUS       DAOSXM010000112         6192 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2111
            NODE_8324_length_6192_cov_10.772038, whole genome shotgun sequence.
ACCESSION   DAOSXM010000112 DAOSXM010000000
VERSION     DAOSXM010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864178
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 6192)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6192)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:24:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,261
            CDSs (total)                      :: 2,229
            Genes (coding)                    :: 2,218
            CDSs (with protein)               :: 2,218
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 3 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6192
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8324_length_6192_cov_10.772038"
                     /isolate="AMR_MDS_2111"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..755)
                     /locus_tag="PLQ68_10250"
     CDS             complement(<1..755)
                     /locus_tag="PLQ68_10250"
                     /inference="COORDINATES: protein motif:HMM:NF015772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HXK73075.1"
                     /translation="MVYCKNCGAQISDNASFCTNCGQKVEKDLVCRNCNSKQKPGTQF
                     CPVCGTPVTEHRLPIQTPVKKPIKNLKKKILISAIAIVLVASIILGIMYVPGMINRNK
                     TYKTDQGKAQKGTVVTVQTDNPVVQAEGITVDFGSFAPQENIELEISQVNGATDEEMG
                     EALASYRLTVDDEEHTEFASFIGITIPIDMTNYDDIDVCATYYDEDTSKWVMLPHETS
                     KEENKIIIYTDHFSDFSVFNISRSNTRFASASL"
     gene            1000..1953
                     /locus_tag="PLQ68_10255"
     CDS             1000..1953
                     /locus_tag="PLQ68_10255"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HXK73076.1"
                     /translation="MNEYNYSKELGKPISFGDINFEKPLVLFFMKLGCNLSNLQIKPF
                     KAEDGLKKSIIKVPVGNNEKIKCTVIEPSDSKELLPAIIYLHGGAFVCPIVSLMIQNA
                     AYYAKALNCRVYIPQYRLAPKYPFPIPLYDCYFSYNHIIENSHKYLIDKTNVLIYGDS
                     AGGCLAASTCHMLRDNKKHIPNAQILVYPVTDNSLTSKSIEQFKNAAWTKTANIHMWN
                     CYLKNGHKNMLGYAAPLNNSNFSKLPPAYVEALEIDTLRDEAIAYADKLRSEGISVEK
                     HLVEGAYHGFDIDHKSPLVKKVLEYRVKVMRNYIKTNIPEH"
     gene            complement(1963..2760)
                     /gene="zupT"
                     /locus_tag="PLQ68_10260"
     CDS             complement(1963..2760)
                     /gene="zupT"
                     /locus_tag="PLQ68_10260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016206155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HXK73077.1"
                     /translation="MGNIWLAFGLTCIAGLSTGIGSLIAFFAKKTNTKFLSISLGFSA
                     GVMIYVSMIEIFPKAAKTISQTHGEKNGVLFAIIAFFLGMFLIGIIDKMIPSEKNPHE
                     VKFVESENEEIKKNKLKRMGVLTALAVAIHNFPEGLATFVAALQEPKVAIPIVAAIAI
                     HNIPEGIAVSVPIYYATGSKRKAFLYSFASGLSEPVGALVGYLILMPFMNDTVFGILY
                     ASVAGIMVFISIDELLPSAREYGEHHLSIYGMIAGMIVMALSLWLFI"
     gene            2915..3754
                     /locus_tag="PLQ68_10265"
     CDS             2915..3754
                     /locus_tag="PLQ68_10265"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HXK73078.1"
                     /translation="MNHIMEFNGLQRNRNYKKILKIAIISFTVIALLIPFLIWQNNSI
                     VVTEYEKHSDKLSNSFDGFTIVHLSDLHSKDYKGKGITKVVPINPDIIVITGDLIDKY
                     DKDLTVAEEAIKKIISIAPVYFVTGNHENLSSLYPALLDIFDKYGVTVLDNEQQTISI
                     DNESISIYGFADTEYDINTNISLDQDVFNLVLCHKPYSPELFNTINADLVLCGHAHGG
                     QIRIPFIGGLIAPDQGFFPKYSEGMHDFSNTSVIISRGLGNSIFPFRLFNRPEIVVIT
                     LHR"
     gene            3829..5199
                     /locus_tag="PLQ68_10270"
     CDS             3829..5199
                     /locus_tag="PLQ68_10270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002608397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HXK73079.1"
                     /translation="MNISQLIQSQRVFFNSDVTKDLSFRLDALNKLYDSIVLNEEKIL
                     EALKSDLNKSSFEGYMSEIGMVRDELRYFIKNLKKWVKPKRVSSPLAQFPSKSYIIAE
                     PYGVVLIMAPWNYPFQLCIEPLIGAIGSGNCVIVKPSAYAPATSAIIAKIIENIFPSD
                     YVAVVEGGRRENSELLDQKFDYIFFTGSISVGKIVMESASKHLTPVSLELGGKSPAIV
                     DQNTDVQLSAKRIAFGKYLNAGQTCVAPDYVLVHESNKESFLKALSSSITEFFGDNPL
                     INKDLPKIINEKHFMRLLDLMKDEFVVCGGKNDKTKLLIEPTVLSDITYDSPIMQEEI
                     FGPILPIITFSDINQVIQDLKFKDKPLALYIFSNNPYIQKKVTESLSFGGGCINDTIV
                     HLATSKMGFGGVGTSGMGSYHGKFSFDTFTHYKGILNKARWLDLPMRYHPYNEKNFKM
                     IKMFLK"
     gene            complement(5227..6129)
                     /locus_tag="PLQ68_10275"
     CDS             complement(5227..6129)
                     /locus_tag="PLQ68_10275"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HXK73080.1"
                     /translation="MERKNKVFAHIIALITIIAWGSTFIASRFMLDYFTPLQVMTMRF
                     VIAYVVLWIMKPKLTHLIWKDELVFLLMGLSGCSIYFITENTALTITSTSNVSILVAS
                     SPILTALLAHLFTKDEKLKPNTITGFIIAFIGVAFVVFNGTFILKLNPIGDLLAFFSA
                     LSWAVYSIILKNVINRYDNILLIRKIMFYGILTAAPFLFIEGKRFDYEALLNPKILIS
                     ILFLGVIGSGICYLTWNISVKRLGIITTNSYIYVIPFVTMILAAILLKEQITIIGVLG
                     AVLIISGVVITSSPKNIMVRRCKP"
BASE COUNT         2162 a         1065 c          967 g         1998 t
ORIGIN      
        1 ttaagagaag ccgaagcaaa ccttgtattg gaccttgata tattaaaaac ggaaaaatcg
       61 gaaaaatgat ctgtataaat aattatttta ttttcttctt tagatgtttc atgaggaagc
      121 attacccact ttgaggtatc ttcatcatag tatgtcgcac aaacatcaat atcatcataa
      181 ttcgtcatat ctatgggaat ggtaattcct ataaagctgg caaattcagt atgctcttca
      241 tcatctacag ttagtctgta tgatgctaaa gcttcaccca tttcttcatc tgtagcccca
      301 tttacctgac ttatctcaag ctcaatattt tcttgtggcg caaaacttcc aaaatcaaca
      361 gtaattcctt ctgcctgcac aacagggtta tctgtctgta ctgttacgac tgtacctttt
      421 tgagcctttc cctgatcagt cttgtatgtc ttattgcgat taatcattcc aggaacatac
      481 attattccca aaattatact tgcaaccagt acaatagcga tagcagatat aagtatcttt
      541 ttcttaagat tcttgattgg ttttttcaca ggtgtttgaa taggaagtct gtgctcagtt
      601 acaggtgttc cgcataccgg acagaactga gtccctggtt tttgtttact attacaattt
      661 ctacatacta aatctttttc aactttctgt ccgcaattag tgcaaaaaga agcattatca
      721 ctaatctgtg ctccacaatt tttacaatac accatatcta cccccgcttt atgataatat
      781 attgagttgt taatacagac tcattatatc actctttttt ttctttccat agttattttt
      841 tgtatctata ttttaatact aactacttaa ctaattactc cttcatatat caattcgatg
      901 tatgcatatg gtaaaatatt gataaatatg aatatattta gtcattaaga attaaatatt
      961 aagataagta tgcataaaga aaccgggaga acggattaca tgaacgaata taactatagc
     1021 aaggaactgg ggaaacctat aagtttcgga gatataaatt ttgagaagcc tttggttctt
     1081 ttttttatga agcttggctg caatttatcc aatctacaga taaaaccttt taaagcagaa
     1141 gatggattaa aaaagagcat tataaaagta cctgttggga acaacgaaaa gataaaatgt
     1201 acagtaatag aaccttctga ctcaaaagaa ctgctacctg ccattattta tcttcatggt
     1261 ggagcttttg tatgccctat agtaagcctt atgattcaaa atgcagcata ttatgcaaaa
     1321 gcattgaact gcagagtata tatcccgcaa taccgtcttg ctccaaaata cccattccct
     1381 attcctttat atgactgcta tttttcatat aatcacatta ttgaaaactc tcataaatat
     1441 ttaatagata aaactaatgt actaatatat ggagacagtg caggagggtg tctggcagca
     1501 agtacttgcc atatgctaag agataataaa aagcatatac ctaatgccca gatattagtt
     1561 tatccagtaa cagacaattc cctgacttca aaatctattg agcagtttaa aaatgctgct
     1621 tggacaaaaa cagctaatat tcatatgtgg aattgctatc ttaaaaatgg tcataaaaat
     1681 atgttaggtt atgcagcacc tttgaataat tccaatttca gcaaactgcc tcctgcttat
     1741 gtcgaagcgc ttgaaattga tactttgaga gatgaagcga tagcctatgc agacaaactc
     1801 agaagtgaag gaatatctgt tgaaaaacat ttagttgaag gagcatatca tggatttgac
     1861 atagatcaca aatccccttt agtaaaaaaa gtccttgaat atcgtgtaaa agtaatgagg
     1921 aattacataa agacaaatat acctgaacat taactattaa aactagataa acaaccaaag
     1981 actcaatgcc attactatca ttcccgctat cattccgtaa atagaaagat gatgctctcc
     2041 gtattctctt gcagagggta ataactcatc aatagatatg aataccatta ttcctgctac
     2101 agatgcatac agaattccaa aaactgtgtc attcataaag ggcattaata tcagataccc
     2161 tactaatgca cctacaggct cagaaagccc tgatgcgaaa gaatacaaaa aagcttttct
     2221 cttacttcct gtagcataat aaataggaac agaaacagca ataccttctg gaatattgtg
     2281 gattgcaata gctgctacaa tcggtatagc aacttttggt tcctgaagag cagctacaaa
     2341 agtagcaagt ccttctggaa aattgtggat agctacagct aatgccgtaa gcacacccat
     2401 tcttttcagc ttatttttct ttatctcttc attctctgac tctacaaatt taacctcatg
     2461 agggttcttc tcgcttggta tcattttatc aataatacca atcagaaaca ttcctaggaa
     2521 aaatgctatt attgcaaata gtactccatt tttttctcca tgtgtttgcg aaatagtttt
     2581 agcagcctta ggaaatatct ctatcatcga tacatatatc attacccctg ctgaaaaacc
     2641 taaactaatg gataaaaact tagtattggt ttttttagca aaaaaagcga taaggcttcc
     2701 tattcctgtt gataacccag ctatgcatgt taatccaaat gccaaccata tattacccat
     2761 atatcatccc tcacaatttt ctatataaaa gtatatcata gttatataat tatcaataag
     2821 agttattgga caattaataa ctattttcat ttggaagaag aaatttggat gaattgaaat
     2881 aaaagtctta ctttttcttt cagttaaggg tataatgaat cacattatgg agtttaacgg
     2941 cttacaacgt aacagaaact ataaaaaaat cttaaaaatc gcaataattt cttttactgt
     3001 aatagcattg cttataccct ttctaatatg gcaaaacaac agcattgttg ttactgaata
     3061 tgaaaaacat agtgataaat tgagtaactc atttgatggt tttacaattg tacacttatc
     3121 tgatttacat agtaaggatt ataaaggcaa aggaataaca aaggttgtac ctattaatcc
     3181 tgatattatc gttataactg gagatcttat cgataaatat gataaagatt taactgtcgc
     3241 agaagaagct ataaaaaaaa ttataagcat tgctcctgtt tacttcgtta caggcaacca
     3301 tgaaaactta tcttctcttt atcctgcttt attagatatt tttgacaagt acggagtaac
     3361 agttcttgat aatgaacaac aaacaatatc cattgacaat gaaagcattt caatttatgg
     3421 atttgccgat actgaatatg atattaatac aaacatatct cttgaccagg atgttttcaa
     3481 ccttgtatta tgtcacaaac catattcacc agaattattt aacacaatta atgctgatct
     3541 tgtattatgc ggacatgctc atggaggcca aatacgtatc cctttcattg gaggacttat
     3601 tgctcctgac cagggttttt ttccgaaata ctcagagggg atgcatgatt tttctaatac
     3661 ttcagtaatt ataagcagag ggttagggaa tagtattttt ccattcagac ttttcaacag
     3721 accagaaatt gttgtaataa cccttcatag gtaatcgtat tccttacaaa ttgatatttg
     3781 gtgtataatg tatatatatt ctaaaattat aatactggag gcgattttat gaacatatca
     3841 caacttattc aatcacaacg tgtatttttt aattctgatg taacaaaaga cttgagtttc
     3901 aggcttgatg cactaaacaa attgtatgat tccatagttt taaatgagga aaaaatacta
     3961 gaagccttaa agtctgatct aaataaatcg tcttttgaag ggtatatgtc tgaaatagga
     4021 atggtaaggg atgaattgag atactttata aaaaacttaa aaaaatgggt taaaccaaaa
     4081 agagtttcat cacctcttgc acagtttcca tcaaagagtt atataatagc cgaaccatac
     4141 ggagttgttt taataatggc tccatggaac taccctttcc aattatgtat tgagccttta
     4201 ataggagcaa ttggtagcgg taactgcgta atagtaaaac cttctgcata tgcacctgca
     4261 acatctgcaa ttattgccaa gataatcgaa aatatattcc cttctgacta tgttgctgtc
     4321 gttgaaggcg gaagaaggga aaattctgaa ttattagatc agaaatttga ttatatattc
     4381 ttcacaggaa gtataagtgt tggaaaaata gtcatggaat cagcatccaa gcatcttacg
     4441 cctgtaagtc tggaattagg cggtaaaagc cctgcaattg tagaccaaaa cactgatgta
     4501 cagttatctg caaagcgcat agcttttggt aaatacttaa atgccggaca gacttgtgta
     4561 gctcctgatt atgtgctagt tcacgaaagc aataaagaat ctttcttaaa agctttatct
     4621 tcttcaatta ctgaattttt tggagataat cccctaataa acaaagattt gccaaaaata
     4681 attaatgaaa aacacttcat gaggcttctt gatttgatga aagatgagtt tgttgtgtgt
     4741 ggcggaaaga atgataaaac caaattatta atcgaaccca cggttttatc agatatcact
     4801 tatgattcac ccattatgca agaagaaata ttcggaccta ttcttcccat aataaccttt
     4861 agtgatataa atcaggttat tcaggatttg aaatttaagg acaaaccatt agctttatac
     4921 attttttcaa acaacccgta catacaaaaa aaggtaactg aaagtctttc attcggcgga
     4981 ggatgcataa atgatacaat agttcatctt gcaacatcaa aaatgggatt tggcggagtt
     5041 ggaacaagtg gaatgggttc atatcatggt aaattcagct ttgatacttt tacacattac
     5101 aaggggatat tgaataaagc acgctggctg gaccttccga tgagatatca cccatacaat
     5161 gagaaaaact tcaaaatgat taaaatgttt ttaaaataga aatttaattt ataaatttaa
     5221 aaagcatcat ggtttacatc gtctgaccat gatgtttttt ggtgaacttg taataacaac
     5281 accagaaata atgaggactg cacctaaaac tccaattata gtaatctgct cttttaacag
     5341 aatagcggct agaatcattg ttacaaaagg aattacatat atataactgt ttgtagtaat
     5401 tataccaagt ctcttaacag atatattcca agtcagataa caaatcccgc ttccaattac
     5461 tccgagaaat aaaatactta tcagtatttt aggatttagt aaagcttcat agtcaaacct
     5521 tttaccttct ataaagagaa aaggtgccgc agttaagatt ccataaaaca taatcttcct
     5581 tattagaagt atgttgtcat atcggtttat tacattctta agaattattg agtaaaccgc
     5641 ccatgataaa gcagaaaaga aagccagaag atcacctatt gggttaagct tcaaaatgaa
     5701 tgttccattg aaaacaacaa aagctacacc aataaatgca attataaacc ctgttattgt
     5761 attgggttta agtttttcat cttttgtaaa caaatgagct aaaagtgcag tgagaattgg
     5821 tgaagatgct actaaaatgc ttacatttga tgttgatgtt atagtcaatg ctgtattttc
     5881 tgttataaag taaatggagc atccagataa tcccataaga agaaatacca gctcatcttt
     5941 ccagattaaa tgcgtaagct taggcttcat tatccataag actacataag caattacgaa
     6001 tctcatagtc attacctgta aaggagtaaa atagtcaagc ataaatctac tggcaataaa
     6061 tgtactaccc catgctatta tcgttatcaa tgctattata tgtgcaaaaa ccttattttt
     6121 tcgttccata aataatcctt ttccaacctg ctcattatat cagaagcatg aaaaaatgaa
     6181 atatacaact ta
//
LOCUS       DAOSXM010000113         5866 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2111
            NODE_9011_length_5866_cov_10.807950, whole genome shotgun sequence.
ACCESSION   DAOSXM010000113 DAOSXM010000000
VERSION     DAOSXM010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864178
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5866)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5866)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:24:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,261
            CDSs (total)                      :: 2,229
            Genes (coding)                    :: 2,218
            CDSs (with protein)               :: 2,218
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 3 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5866
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9011_length_5866_cov_10.807950"
                     /isolate="AMR_MDS_2111"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..191
                     /locus_tag="PLQ68_10280"
     CDS             <1..191
                     /locus_tag="PLQ68_10280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK73081.1"
                     /translation="FFTISGAETVFSTAVFTYSSHNPSVNNIILFYMHINRLSTAIIF
                     FTSKCSYLVDRKVLCDVK"
     gene            214..1803
                     /locus_tag="PLQ68_10285"
     CDS             214..1803
                     /locus_tag="PLQ68_10285"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HXK73082.1"
                     /translation="MAIKHIFVTGGVVSGLGKGITAASLGRLLKTMGYKVALQKFDPY
                     INVDPGIMSPYQHGEVYLTEDGIETDLDLGHYERFIDENLDHLSSFTAGKIYDTIRNR
                     EHAGAYRGGTIQVIPHITNEIKSLIYKNAEEKGVQVAITEIGGTVGDIESQPFLEAIR
                     QVGLEKGHENVLYVHVTLLPYLKISDELKTKPTQHSVKELRSLGIQPDIIVCRSEKHI
                     SRAIKDKIALFCDVEPDCVIENIDAASIYEIPVLLANEGFADIVVKKLNLKKRNLDLS
                     EWNKIIYELNNPQKTVKIALVGQYVAFHESYLSICEALKHGGFHNHSTIDIKWIDPVD
                     VNSDNVAEVFKDISGILIPAGFKDRGIEGMILTANFARTNNIPYMGLCLGMHTAVIEF
                     ARNVCDMQDAHSTEFKSNTKYDVITSNENESRLGGYVCDPVKNSKLSNIYGSCKPFSE
                     RHKHKYEINTEYTGIMEKNGLKVSGMHKNLVDAMELEQHPFYILTQYQPEFKSRPTNP
                     HPLMKAFIKAALDYSKKDEDE"
     gene            1796..>2079
                     /locus_tag="PLQ68_10290"
     CDS             1796..>2079
                     /locus_tag="PLQ68_10290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK73083.1"
                     /translation="MNKDSKKNKLKDNKKLITNIALFGAFPIICLVFALIGKWLLFGA
                     VLTIYMAVFIYLKLENLRLIATGYYDKKGDYQKALKPAYKAYKMKNSSIDT"
     assembly_gap    2080..2179
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2180..2380
                     /locus_tag="PLQ68_10295"
     CDS             <2180..2380
                     /locus_tag="PLQ68_10295"
                     /inference="COORDINATES: protein motif:HMM:NF019339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXK73084.1"
                     /translation="LLEEPVSFPEAYYNAALVSHSQGNYEEAIRLFKQALSKPFSGLT
                     AVTQDEIQTKIDYLNRGQGGMV"
     gene            complement(2347..3006)
                     /locus_tag="PLQ68_10300"
     CDS             complement(2347..3006)
                     /locus_tag="PLQ68_10300"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004625945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HXK73085.1"
                     /translation="MNIIYEDNHIIVVIKKADQPVMLDSSNDYDLLTEIKDYIKIKYN
                     KPGNVYTGLVHRLDRPVGGVMVFAKTSKAASRLSNSIRNIEFDKKYYAVVKGIINPLE
                     ATIENWMCKDDQAKKSYICNDDTPNAKLAKLKYKVIKSVSDLSLLDIELLTGRHHQIR
                     VQLSSAGYPIYGDMRYGDGPKGHIALFAYSLGFIHPVTQKPMVFECDPDYTIPPWPLF
                     K"
     gene            complement(3003..3878)
                     /locus_tag="PLQ68_10305"
     CDS             complement(3003..3878)
                     /locus_tag="PLQ68_10305"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HXK73086.1"
                     /translation="MICISKDWKDYELLETGDQQKTERWKDIILRRPDPQCIWPYPSG
                     SKLLPHGIYNRSNSGGGSWTWNKPIPKRWKISYKELKFFVEPTGFKHTGLFPEQAYNW
                     DFLIDKINNEYERRTDEINVLNLFAYTGGATVACAYAKAAVCHVDASKGMVTRAKENA
                     QLSGLADAKIRYIVDDVVKFVRREINRNSKYDIIIMDPPTYGRGPKGEMWQVEDSLYN
                     LVELCLQVMSDSPIAFLINSYASDISMTSIENILKKTVMNKFSGQTKSYELGLIQSDG
                     KLILPCGYTARWENE"
     gene            4002..5018
                     /locus_tag="PLQ68_10310"
     CDS             4002..5018
                     /locus_tag="PLQ68_10310"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HXK73087.1"
                     /translation="MDSRTRVINVLKHESIDRFPRTLWLLPNVQIFKKDLHDNLFSTM
                     EFDIANPNLGFKKSRYEKGTPYLMPGYVDEFGCTFEVAEQGVVGEVKNPIFTELDIID
                     RYIMPYEILEGFDKDNINRQCINSDKFMLANTHIRPFERAQFMRGTEDLFMDLAMEEP
                     KIKKLLSKLHHFYLKELDMITDTMIDGFSFMDDWGTQISLLINPQIWREIFKPMYKDY
                     CDLAHSKNKYVFFHSDGFIEQIYPDLVEIGVDAINSQLFCMDIEKLVDLYGDKIAFWG
                     EIDRQYILPFGTKDDVKNAVDRVSEAVIKKNKKRTGAFAQCEWNAFDPYENMITVFEH
                     WNTK"
     gene            complement(5019..5549)
                     /locus_tag="PLQ68_10315"
     CDS             complement(5019..5549)
                     /locus_tag="PLQ68_10315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004463438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HXK73088.1"
                     /translation="MKGLIQIYTGDGKGKTTACVGAAIRACGHGMTVYFVQFQKGSDS
                     GELKVLRKIDNINVNRICSFKKFYYYMNEEEKSLYAKEHKQAFRSVVETISKNPDKYD
                     MLIMDELLGAVSIGVIDEDEVVEFLTNKPEHLEILLSGRNASDKIIQVSDYVSDIKCL
                     KHPFEKNIAARQGIEY"
     gene            complement(5558..>5866)
                     /locus_tag="PLQ68_10320"
     CDS             complement(5558..>5866)
                     /locus_tag="PLQ68_10320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010251447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HXK73089.1"
                     /translation="IREAMKLVAKQGTASRVFSKYPMEVAAKTGTAETGYEIDSSSNG
                     LSIAYAPADNPEIAVCVVIEKGVWGSYTVPAIMRIFNHYFDIELEEEIIDDTLVTNEQ
                     "
BASE COUNT         1890 a         1021 c          961 g         1894 t
ORIGIN      
        1 cattcttcac aatatctgga gccgaaactg tttttagtac cgcagttttt acatactcga
       61 gccataatcc ctccgttaat aacataatat tattctatat gcacatcaat cgtttatcaa
      121 ctgctatcat tttttttact agtaaatgct cttatctggt tgacagaaaa gtgctttgcg
      181 atgtaaaata aaaaaaaccg tatggggtgg agaatggcaa taaaacatat atttgtaact
      241 ggcggcgttg tatccggatt gggaaagggc ataacagccg cttctttagg tagattactc
      301 aaaactatgg ggtataaagt agccctgcag aaatttgatc catacataaa tgttgatcct
      361 ggcataatga gtccttatca gcacggcgaa gtttacctta cagaagatgg tatcgaaaca
      421 gatttagact taggacatta tgaaagattt atagatgaga atcttgatca cttaagcagt
      481 tttacagctg gtaaaatata tgacactata agaaacagag agcacgctgg agcatataga
      541 ggaggaacaa ttcaagtcat tcctcatata actaatgaaa ttaaaagttt aatatacaaa
      601 aatgctgaag aaaaaggcgt acaagttgca ataactgaaa taggtggaac tgtaggcgat
      661 atagaatcac agcctttctt ggaagcaata agacaggttg gccttgaaaa aggtcatgaa
      721 aatgtccttt atgtgcatgt tacccttttg ccgtatttaa aaatatccga tgagttgaaa
      781 acaaagccaa cccagcattc tgttaaagag ttaagaagtt taggtataca gccggatatt
      841 attgtatgta ggtccgaaaa acatatatca agagctatta aggataagat agcgctgttt
      901 tgtgatgttg aaccagattg tgtaatagaa aacatcgatg cagcaagcat atatgaaatc
      961 ccagttcttc ttgctaatga gggatttgct gatatagttg tcaagaagct taatttaaag
     1021 aaacgaaatc tggatttatc agaatggaat aaaattattt atgaattaaa caatcctcag
     1081 aagactgtaa aaatcgcact tgttggccag tatgtcgcat tccacgagtc atatttatct
     1141 atatgcgaag cactgaagca tggaggtttc cacaatcatt caactattga catcaagtgg
     1201 attgacccag ttgatgttaa cagtgataat gttgctgagg tattcaaaga tataagtgga
     1261 attttaatac ctgccggttt taaggataga ggaattgaag gtatgatatt aactgctaat
     1321 tttgcaagaa caaataatat accttatatg ggactatgct taggcatgca cactgcggtt
     1381 attgaatttg ctagaaatgt ttgtgatatg caagatgcac atagtacaga gtttaaaagt
     1441 aatactaagt atgatgttat aacctccaat gaaaatgaat ccagacttgg cggatatgta
     1501 tgcgatccag taaaaaattc taaactatca aatatatacg gaagctgcaa accattttct
     1561 gaaagacata agcacaaata tgagataaat acagaatata ctggtataat ggaaaagaac
     1621 ggtttaaaag tcagcggtat gcataaaaat cttgttgacg ctatggaact tgaacagcat
     1681 cctttttata tacttacaca gtatcagcca gagttcaagt cacgtccaac aaatcctcat
     1741 ccactgatga aagcctttat aaaagcagca ctagattatt caaagaagga tgaagatgaa
     1801 taaagacagt aaaaaaaata agctaaaaga taataaaaaa ctgattacaa acatagcact
     1861 atttggagca tttccaatca tctgccttgt tttcgcactc ataggtaaat ggctattgtt
     1921 cggagcagtt ctgacaatat atatggctgt attcatttat cttaaactgg agaatttaag
     1981 actcattgct actggatatt atgataaaaa aggtgattat cagaaagctc ttaaaccagc
     2041 atataaggca tataagatga agaattcatc tattgatacn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnc ttctagaaga gccagtcagt tttcctgaag cgtattacaa
     2221 tgcagcgctt gtatctcatt cacaaggaaa ttacgaagaa gctataaggt tgttcaaaca
     2281 agccctttca aaaccgttta gcggtttaac tgctgtcaca caagatgaaa ttcaaacaaa
     2341 aattgattat ttaaatagag gccagggagg aatggtgtaa tcaggatcac attcaaaaac
     2401 cataggtttt tgtgtaaccg gatgaataaa tcctaagcta tatgcaaaca atgcaatgtg
     2461 gcctttcggt ccatctccat atctcatatc accatatatc ggataacctg cactagacaa
     2521 ctgaactctt atctgatgat gccttccagt taataattca atgtcaagca gtgacaaatc
     2581 tgaaacagat ttaataactt tatacttcaa ttttgcaagt tttgcattag gtgtgtcgtc
     2641 attacatata tatgattttt ttgcttgatc atccttacac atccaatttt caatagttgc
     2701 ttccaaaggg tttattatac cctttacaac tgcataatat tttttatcaa actcaatatt
     2761 tctaatactg ttacttaatc ttgaagcggc ttttgatgtt tttgcaaaga ccataacacc
     2821 cccaacaggc ctgtcgagcc tgtgtaccag acctgtatag acattacctg gtttgttgta
     2881 tttaatttta atgtaatctt ttatttctgt taataaatca taatcatttg aggaatcgag
     2941 catgacaggc tggtcagctt tttttataac gactattatg tgattatcct catatattat
     3001 attcattcat tctcccacct tgctgtatat ccacatggca agataagttt tccatcagac
     3061 tggattagtc cgagttcata tgattttgtt tgtcctgaaa acttattcat aactgttttt
     3121 ttaagtatat tttcaataga tgtcatagaa atatctgatg cataagagtt aataagaaaa
     3181 gctataggcg agtcactcat aacctgcaag cacagttcca cgagattata taaactgtcc
     3241 tctacctgcc acatctctcc tttcggccca cgtccataag ttggcggatc cattattatt
     3301 atgtcatatt tactgtttct gtttatttct cttcttacga atttaacaac atcatcaact
     3361 atatatctta tttttgcatc tgccaaacct gataactgag cattctcttt tgctctggta
     3421 accatacctt ttgatgcatc aacatgacat actgctgctt ttgcatatgc acaagctact
     3481 gttgcacctc ctgtataagc aaaaaggtta agaacattaa tttcatcagt tcttctttca
     3541 tattcattat ttatcttatc tataagaaaa tcccagttat atgcctgctc tggaaataat
     3601 cctgtgtgtt taaatcctgt aggttcaaca aaaaatttaa gttccttata actgattttc
     3661 caccttttcg gtataggttt attccaagtc caacttcctc cacctgaatt tgaacggtta
     3721 tatataccat gcggaagtaa cttactacca gaaggatatg gccatatgca ttggggatca
     3781 ggccttctta gtataatgtc cttccatctt tccgttttct gttgatcgcc agtttccagc
     3841 aactcataat ctttccagtc tttagatatg caaatcatct gtttacctca tataattatt
     3901 ctatattata ctttattatt atgcaaagat gagtatatta tcaaaaatag ttagattttt
     3961 gttttattta tgatatactt tttctgtttg gaggaaaaaa tatggattca cgtactagag
     4021 tcataaatgt tttaaaacac gaaagcatag acagattccc acgtacttta tggttattac
     4081 caaatgttca gatattcaag aaagatttgc atgacaactt gttttccact atggaatttg
     4141 acatagcaaa tccaaatctt ggctttaaaa aaagcagata tgaaaaaggt actccttatc
     4201 ttatgccagg atatgttgat gaattcggat gtacttttga agttgcagaa caaggtgtag
     4261 ttggagaagt taaaaatcca atttttactg agcttgatat tattgacaga tacataatgc
     4321 catatgagat attagaaggt tttgataagg ataatataaa ccgccaatgt attaattcag
     4381 ataagtttat gcttgcaaat acacatataa gaccatttga aagagcccag tttatgagag
     4441 gtactgaaga tctttttatg gatcttgcta tggaagaacc aaaaataaag aaacttcttt
     4501 ctaaactcca tcatttttat ctaaaggaac tagatatgat tactgataca atgattgatg
     4561 gattttcttt tatggatgac tggggaaccc agatatcctt attaattaat cctcagatat
     4621 ggagagagat attcaagcct atgtataaag actactgcga tttagcacac tcaaaaaata
     4681 agtatgtatt ctttcattca gatggcttta ttgagcagat atatcccgat cttgttgaga
     4741 taggagttga tgcgattaat tcacaactct tctgtatgga tattgaaaag cttgttgacc
     4801 tatatggtga taaaatagca ttctggggtg aaattgaccg tcaatacatt ctgccttttg
     4861 ggactaaaga tgatgtaaag aatgctgttg atagggtttc agaggctgtt attaagaaga
     4921 ataaaaaacg cactggcgca tttgctcagt gcgaatggaa tgcttttgac ccctatgaga
     4981 atatgattac agtttttgag cactggaaca caaaataact aatattctat tccctgcctt
     5041 gctgctatat tcttttcaaa aggatgcttc agacacttaa tatcagacac ataatctgat
     5101 acttggataa ttttatctga ggcatttcta ccacttagaa gtatttctaa atgttcaggt
     5161 ttattagtta aaaattccac aacctcatct tcatctatta cacctatcga tacggctcct
     5221 aataactcat ccataatcag catatcatac ttatcaggat tttttgatat ggtttcaact
     5281 acagatctaa aagcctgctt atgctcctta gcataaaggg atttctcttc ttcattcata
     5341 taatagtaaa actttttaaa gctacatatc ctattcacat tgatattatc gatttttctt
     5401 aaaaccttta gttccccact atctgaacct ttttgaaact gcacaaaata tacagtcata
     5461 ccatgtccgc atgctcttat cgctgcccct acgcatgcag ttgttttccc tttaccatca
     5521 cctgtataaa tctgaattaa acctttcata tttctcctta ctgttcatta gtgactaaag
     5581 tgtcgtcaat aatctcttct tccagctcta tatcaaaata gtgattaaat attctcataa
     5641 ttgcaggaac agtatacgat ccccaaaccc ctttttctat tacaacacat acagctattt
     5701 caggattatc tgctggagca tatgctattg acaagccgtt tgaagaggag tcgatttcat
     5761 aaccagtctc agcagttcct gtttttgctg caacttccat aggatatttt gaaaacactc
     5821 tgcttgctgt tccctgctta gcaacaagtt tcatagcttc tcttat
//
LOCUS       DAOSXM010000114         5676 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2111
            NODE_9446_length_5676_cov_11.611813, whole genome shotgun sequence.
ACCESSION   DAOSXM010000114 DAOSXM010000000
VERSION     DAOSXM010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864178
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5676)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5676)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:24:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,261
            CDSs (total)                      :: 2,229
            Genes (coding)                    :: 2,218
            CDSs (with protein)               :: 2,218
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 3 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5676
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9446_length_5676_cov_11.611813"
                     /isolate="AMR_MDS_2111"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            249..1094
                     /locus_tag="PLQ68_10325"
     CDS             249..1094
                     /locus_tag="PLQ68_10325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008428466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXK73090.1"
                     /translation="MIDVKNVYHDYSNKGHYAVSDITFKMNKGEIFGFLGPSGAGKST
                     MQNIMTGILKIQKGDVQYDGVSIKEQKKDFFNSIGVSFEHPNVYKTLTGYENLKYYSA
                     LYSVDTLDPMMLLDSVGLKEDANKKAGEYSKGMKQRLTFVRSLINNPKYMFLDEPTNG
                     LDPSNSLLVQKIILQQKERGATIILTTHNMELADRLCDTVAFISEGKVEAIDSPNALK
                     LKYGTKQVKIDYLTDNKEKSTIIPLDEKERISDFLMKNEVLKMHTSEATLEDIFIKVT
                     GKELI"
     gene            1094..2062
                     /locus_tag="PLQ68_10330"
     CDS             1094..2062
                     /locus_tag="PLQ68_10330"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXK73091.1"
                     /translation="MKGFFSLWMLDIKRLLKNSLFIVISVTLVLIIIVTLFLLPKDIQ
                     MEEHELVTFGMSEYGFDEVESMDLLYDAVDNGSIGIYLNEDNMPVIVHNGISDRNLNA
                     IAVMVFSKGFTQIESLKLYEDQQPIPFNQRMVPVFIAFEALIVGLILVGALMLAEKEE
                     STLLALRISPIGVNLYILSKTTLLALIGTVYSLLMAITCVGFNYNFLSLIAISFFGSC
                     IFILLGMAFSVFFKTLGEWFFSIAIILMVNMMPVMSYMMPSYSPGWIKLTPSYYILFE
                     LEKILFKQPGQILPVILSMGTWLLVSYVITYQFIRIFYLKRGGRNA"
     gene            2059..3054
                     /locus_tag="PLQ68_10335"
     CDS             2059..3054
                     /locus_tag="PLQ68_10335"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXK73092.1"
                     /translation="MKGLWTNICREFKIITRDGISIALLGAPALLSVIMLAIFGQISG
                     TSASFVTDKSLTDEQKTRLEAVGDVEYVEDFLSLEKRIKGMDSVAGVYSKDSQIFILT
                     EGNEEQGFDQGMSYLVSKALAEDPVEYIKEEVVSKTPMAYDIAIVSVILLAMFIGGSL
                     MGLNGVAERETKVIFAYSISPIGLWGFVAQKLISATIYALLSVAIAAIIMGKSEMLLM
                     LLLVTLFSTLFTGFITFITPAMAENQVSAVGVMKLLMPLSMIIGISSVFVPDKWHFLY
                     YLLPMYWQYTAIQNVLDNKGAILNMILTAVTAIPWFIAAVMLFSKKTNMVARRNK"
     gene            3054..3191
                     /locus_tag="PLQ68_10340"
     CDS             3054..3191
                     /locus_tag="PLQ68_10340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK73093.1"
                     /translation="MQWYWWVLIGLAVVVLVFLKVKVGGAWLKKQKEKKEHKERNLEE
                     D"
     gene            3191..3814
                     /locus_tag="PLQ68_10345"
     CDS             3191..3814
                     /locus_tag="PLQ68_10345"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HXK73094.1"
                     /translation="MKGIKWFDDKKENPLETNRLLLESTLDEFSKCSYNEASLNEIIK
                     RADMRKGSFYYRFYDKKDLYLSLFYYIGMRKAEMINSNATTSQKDDFFASLREKTILA
                     LKFARMEKRYYDFWRIYIKESEEVKNFVHECFGGYYTDEIYTWVEEAKKTGKIRSDVN
                     SKLLADFINILIERIDLLISPEQTDEQIYENVCTMMNLIEHGIKKTG"
     gene            complement(3887..4672)
                     /locus_tag="PLQ68_10350"
     CDS             complement(3887..4672)
                     /locus_tag="PLQ68_10350"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXK73095.1"
                     /translation="MKYSKSNLSDNGSILLLALSSSYVYHNTSWAKNIWDEMSLCDKS
                     MYNEVSGATADEIHELIVRNKHWHENNITNFSEAFPPEFIENFINTISDEYVNWKIQH
                     EVTYYNSSLCVFEDFIDIPLMSDGELRLECIARKPAIPEKKWVPGYVFKNCTNDNCVG
                     ELNVRIGYTDSLYYGGQIGYGIDEQYRGHHYAERACRLLVPLLKSHEMGRVLITNNHT
                     NNASKRTCERLGARLIRVAPVPQWHDLYEEGNRLENIFEWKIE"
     gene            4632..5051
                     /locus_tag="PLQ68_10355"
     CDS             4632..5051
                     /locus_tag="PLQ68_10355"
                     /inference="COORDINATES: protein motif:HMM:NF018533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK73096.1"
                     /translation="MDPLSDKFDFEYFKSLYSEEEKKISIKEFLATKQRIPGLGNGVL
                     QDILFNAKIHPRRKMGTLSKEEFFALYTCVKNTLKEMTDKGGRDTEKDLFGNNGGYCT
                     KLSAKTINDPCKVCEGNIIKEAYMGGNIYYCPNCQKK"
     gene            5064..5501
                     /locus_tag="PLQ68_10360"
     CDS             5064..5501
                     /locus_tag="PLQ68_10360"
                     /inference="COORDINATES: protein motif:HMM:NF024135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HXK73097.1"
                     /translation="MSKVSIVYATMTKHSKKLALAIAEELGIEAVNVTEESKPEKVNL
                     LYIVGGIYGGTCNPVLVSFVNKLDKDMVEKVVLVTSSASESGRCQKQLKTILHEKEIQ
                     LLDEITCTGGFLFVKLGHPNEKDIQQVVMKAKSIYVQESVTEL"
BASE COUNT         1925 a          864 c         1165 g         1722 t
ORIGIN      
        1 caaaaaatat cattttaagc aaagatggtt atttgagtaa tattttttca ccagagagaa
       61 aaaactttta aataatgaaa cttttttctc aaatgagaaa aaaagcgggt aaaatttttt
      121 gcatttccta ttgaccaagt ggtcaagtag tgataatatg tttttgaccg gttggtcaaa
      181 aaaatgacct tttagtcatt tgtgctaaac agctaagctt aattggctgg caaagaaagg
      241 ggaagaacat gattgatgta aagaatgttt accatgatta ttcaaacaaa gggcattatg
      301 cggtgtccga cataactttt aaaatgaaca aaggagaaat attcggattt ttggggcctt
      361 ccggagcagg aaagtctacg atgcagaata ttatgacagg tattttaaag attcaaaagg
      421 gagatgtcca atatgacgga gtatccataa aagaacagaa aaaggatttc ttcaatagta
      481 tcggagtatc ttttgagcat ccgaatgtat acaagactct tacaggttat gagaacctta
      541 aatattattc agcattgtat tcggttgata ctctagaccc tatgatgctg cttgacagcg
      601 tgggattaaa ggaagatgca aacaaaaaag ctggggagta ttcaaaagga atgaaacaaa
      661 ggctcacttt tgtacgttct ttaataaata atcctaaata tatgtttctt gatgaaccta
      721 ctaatggact ggatccttca aattcattgc ttgttcagaa aataatatta cagcaaaagg
      781 aacgaggagc aactatcatt cttactacgc ataatatgga gcttgctgac aggttatgtg
      841 atacagtcgc attcatatcc gaaggaaaag ttgaagctat agattcacct aatgccttaa
      901 aactgaaata tgggaccaag caggttaaga tagattactt aacagacaat aaagagaaga
      961 gcactattat acctcttgat gaaaaagaga ggataagcga ttttttaatg aaaaatgaag
     1021 tattgaaaat gcatacttct gaagcgacac ttgaggacat attcataaag gtgactggaa
     1081 aggagctgat ttaatgaaag gctttttctc tctttggatg ctggatataa aaaggcttct
     1141 gaagaattcg cttttcatag taatatccgt tacgcttgtt ctgataatta tcgtgacgct
     1201 ttttcttctt ccaaaagata tacagatgga agagcatgag cttgtcacat tcggcatgag
     1261 tgaatatggg ttcgatgaag tggaatcaat ggaccttttg tatgatgcag tggataatgg
     1321 aagtatagga atatacctta atgaagataa tatgcctgtg atagttcata atgggatatc
     1381 agacaggaat ttaaatgcga tagcagttat ggtcttttct aaagggttta cacagattga
     1441 atcattaaag ctttatgaag atcagcaacc tatacctttt aaccagagga tggtgccggt
     1501 attcatagct tttgaagcgc ttatagtagg cttaatactt gtaggagctt tgatgctggc
     1561 tgaaaaagag gaaagtacac tgcttgctct taggatttct ccaataggtg taaatctgta
     1621 tatactgtca aaaacaacgc ttctggcgct tataggcact gtctattctt tgctgatggc
     1681 aataacatgt gtaggattta actataactt tttaagcctt atagctatat ccttctttgg
     1741 ttcctgtatt ttcatactgc ttggaatggc attttcggta ttcttcaaga cgttaggaga
     1801 atggttcttc tcaatagcaa taatattaat ggtgaatatg atgcctgtca tgagctacat
     1861 gatgccgtca tactctcctg gatggataaa attaacacct tcatattaca tactgtttga
     1921 attagagaag atacttttca aacagccagg acagattctt ccagttatat tatcgatggg
     1981 tacatggcta ttagtgtcat atgtaataac ttatcagttt atcaggatat tttatcttaa
     2041 aagaggaggt aggaacgcat gaaaggctta tggacaaata tatgcaggga atttaagata
     2101 ataacccgag acggaatatc tatagctctt ttaggagcac ctgctcttct gtcagtgata
     2161 atgctggcta tattcggaca gattagcggg acatcagctt cttttgtaac agataaaagc
     2221 cttacagatg agcagaagac aagattggaa gcagtaggtg atgtggaata tgttgaagac
     2281 tttttaagtc ttgaaaaacg tataaaaggt atggattcag tagctggcgt atacagtaaa
     2341 gacagtcaga tatttatact tactgaaggc aacgaagagc agggttttga tcagggtatg
     2401 tcatatctcg tatcaaaagc tttggctgaa gaccctgtag agtatattaa agaagaagtt
     2461 gtttccaaga ctccaatggc ctatgatatt gctattgtaa gcgtaattct tttagctatg
     2521 tttataggag ggtctttgat gggtcttaac ggtgtggctg aaagagagac aaaagtaatt
     2581 tttgcatatt caataagccc gataggattg tggggttttg tggcacagaa actcatatca
     2641 gcaacaattt atgccttact gagcgttgca atagctgcaa taatcatggg gaaaagtgaa
     2701 atgcttctga tgcttctatt ggtgacactg ttcagcactt tatttacagg atttataact
     2761 ttcataactc ctgcaatggc agaaaatcag gtatcggcag ttggagttat gaagctttta
     2821 atgcctttat ctatgataat agggatatca tcagtattcg ttcctgacaa atggcacttt
     2881 ttgtactacc tgcttccaat gtactggcag tatacggcaa tacagaatgt actggataac
     2941 aaaggagcga tactgaatat gatattaaca gcagtaacag ctattccatg gtttattgca
     3001 gcagtgatgc tgttttcgaa aaaaacaaat atggtcgcaa ggaggaacaa gtaatgcagt
     3061 ggtattggtg ggtattaata ggtttagcag tagtagtatt agtgttctta aaggttaagg
     3121 taggcggagc ctggcttaag aaacagaaag agaagaaaga acataaagag aggaatctag
     3181 aagaggatta atgaaaggta ttaagtggtt tgatgacaaa aaagagaatc ctttagaaac
     3241 caacagactt cttctggaaa gcactctgga tgaattctca aagtgttcat acaatgaggc
     3301 ctcattgaat gagataatta aaagagctga tatgagaaag ggaagtttct actacagatt
     3361 ctatgacaaa aaggatctgt atctttccct tttctactat atcgggatga gaaaagctga
     3421 aatgataaac agcaatgcaa caacatcaca aaaggatgat ttttttgctt cactcaggga
     3481 aaaaactatt cttgctttaa aatttgcacg catggaaaaa agatattatg acttctggag
     3541 gatatatatt aaagaaagcg aagaggttaa aaactttgtt catgagtgtt tcggaggtta
     3601 ttatactgat gaaatatata cctgggtgga agaagcaaag aaaacaggaa agattcgctc
     3661 cgatgttaac agcaagcttt tagcagattt cataaatatc ctcattgaaa gaattgatct
     3721 tctcatatct ccggagcaga cagatgaaca gatttatgag aatgtctgca ccatgatgaa
     3781 tcttattgag catggcatca agaaaactgg ttaatacagc ataatcacat aaaatcatat
     3841 atttatgtaa ttagaatcac acttttatat ataacagtgt gattaatcac tctattttcc
     3901 attcgaaaat attttccagc ctattcccct cttcatacag gtcgtgccat tgcggtaccg
     3961 gagccacacg tatcagtctc gctccgagcc tttcacatgt cctcttggat gcattgtttg
     4021 tatggttatt agttattaat actcttccca tttcgtgaga ttttaaaaga gggacaagta
     4081 acctgcatgc tctttctgca tagtgatgtc cacgatattg ctcatctatt ccatacccta
     4141 tctgccctcc ataatacagg ctgtctgtat aaccgattcg aacattcagt tctccaacac
     4201 agttatcgtt cgtgcaattt ttaaacacat aaccaggaac ccatttcttt tccggtatag
     4261 caggttttct agctatgcat tcaagtcgca gttcgccatc tgacataaga gggatatcaa
     4321 taaaatcttc aaaaacgcaa agtgaagaat tgtagtaggt aacttcatgt tgtatcttcc
     4381 aattgacata ctcatcagag atagtattta taaaattttc gataaactct ggtggaaagg
     4441 cttcagagaa attagtgata ttgttctcat gccagtgctt gttgcgaact atcagttcat
     4501 gaatttcatc tgctgtagcg cctgatactt cattgtacat tgatttgtcg cataaactca
     4561 tttcatccca tatgttttta gcccatgagg tattatggta gacataactg cttgataaag
     4621 ctagaagcaa aattgatccg ttatctgata agtttgattt tgaatatttc aagtctcttt
     4681 attctgaaga agaaaagaaa atatccataa aagagttttt agcgacgaag cagagaatac
     4741 ctggacttgg aaatggtgtt ttacaggata ttctattcaa tgcaaaaata catccaagac
     4801 gcaaaatggg cacattatcc aaagaagaat tctttgcttt atacacttgt gttaaaaata
     4861 cattgaagga aatgactgat aagggaggaa gagatacaga aaaagatctc tttgggaaca
     4921 atggaggtta ctgtactaaa ctgtccgcaa agacaataaa tgacccatgt aaggtatgcg
     4981 aaggtaacat tattaaagaa gcatatatgg gtggaaacat atattattgt ccgaattgtc
     5041 agaaaaaata gggggaaaag attatgagta aggtaagtat cgtatacgca acaatgacga
     5101 aacattcaaa aaagctggca ttagctattg cagaggaact tggtattgaa gctgtaaatg
     5161 taacagaaga atcaaaacct gaaaaagtta atcttttata cattgtcgga gggatatatg
     5221 gcggaacatg taatcctgtt ttagtatcat ttgttaacaa actggataaa gatatggttg
     5281 aaaaagtggt tcttgtgaca agcagcgcat ctgaaagcgg aagatgccag aaacagttaa
     5341 aaactatatt gcacgagaaa gaaattcagc ttctagatga gataacatgt actggcgggt
     5401 tccttttcgt caagttaggt caccctaatg agaaggatat acagcaggtt gtaatgaaag
     5461 caaagagtat ttatgtacag gaatctgtaa ctgagttata aatgtactaa taaatttatg
     5521 ttggataggc accacttgca tcgacaatgg gtttttatat acgaaaaagg ttaagtaatt
     5581 tatgtttatt ataattatac aataacatta aaacgttaga ttaaagttac tgtagggaaa
     5641 tgaataaaaa gatagagaga caatctttct gttaaa
//
LOCUS       DAOSXM010000115         5374 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2111
            NODE_10201_length_5374_cov_9.282384, whole genome shotgun sequence.
ACCESSION   DAOSXM010000115 DAOSXM010000000
VERSION     DAOSXM010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864178
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5374)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5374)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:24:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,261
            CDSs (total)                      :: 2,229
            Genes (coding)                    :: 2,218
            CDSs (with protein)               :: 2,218
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 3 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5374
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10201_length_5374_cov_9.282384"
                     /isolate="AMR_MDS_2111"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(51..275)
                     /locus_tag="PLQ68_10365"
     CDS             complement(51..275)
                     /locus_tag="PLQ68_10365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002851980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK73098.1"
                     /translation="MFNKIKLKRGIKNSIKLIEELEKKRYRSQSALVEAILKHETPND
                     DDVEYFNKYTAEIDKLRREIRSMKKDLENL"
     gene            complement(284..865)
                     /locus_tag="PLQ68_10370"
     CDS             complement(284..865)
                     /locus_tag="PLQ68_10370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK73099.1"
                     /translation="MMEQQKNPKDPEQKKEKTNLIKRILNSEDMENTDDSDYQSLLRK
                     NFYNFDQVRKNRQLKSLRITTIILTVMLIFFLSLYLVKLNDSTMLLDRVINASLKYVY
                     DNVLYVDNQVIYTEVVSELNVMRTLMNHNGETGKRQFVISELYYIAVQLPDSFVSYRS
                     EVYDAFKNLHEGRLDIGYRLLEELLMKIVQERK"
     gene            complement(801..1448)
                     /locus_tag="PLQ68_10375"
     CDS             complement(801..1448)
                     /locus_tag="PLQ68_10375"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HXK73100.1"
                     /translation="MSEFKGKKKSKWIITAIFAVLLVFLIIFVGNMIYSQITKKVPSF
                     FGYSVMNIISTSMEPQIPENTFILIKKVDPAELKVGDVITFYSKDPTIRGLPNTHRIT
                     DIRIENGNFVFITKGDANAISDAYEVDSSSIIGVYQKNLITLGKAGRIFQSRTFIFIL
                     LVVPAVLLFVFEIINLAKTAKNVEKEKIEGKEADNDGATKESEGSGTEEGKNESD"
     gene            complement(1476..>2559)
                     /locus_tag="PLQ68_10380"
     CDS             complement(1476..>2559)
                     /locus_tag="PLQ68_10380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HXK73101.1"
                     /translation="KKVLQKNIDEIWVNKIEQALPYLDTALRTTRKDIKEVEEIKPIE
                     LTRKITVKSLQHLATHTNFIDSIDEKGNVIPNKLLNVYKDEDIFTYENKFINTLLYKL
                     GIFIDSRYDKLKEYGADEISNVMSLDIDTKVIDTKVKISLTIETNDAADDKEGQSEEK
                     NVNILDDMSKSSLWKRVLRLRKIIRQYQSSQFITQMGRAFIRPPVMRTNAILKNVHLR
                     QCLELWQFIESYESAGFEVESSSTSSKPSTELIENFYKLMAYQYVFFRKNTTDKVDDI
                     INESKAKIKYNARFKKINTEEFADEYEFLNPNVKKVYIEQLSRKNTKLTREELKIRKA
                     IENALKSERLYAAELKKQEKIKNKKK"
     assembly_gap    2560..2659
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2660..2872)
                     /locus_tag="PLQ68_10385"
     CDS             complement(<2660..2872)
                     /locus_tag="PLQ68_10385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK73102.1"
                     /translation="MPRYSKKLVLDDDQKNSIYEDTKDFISYIYEDYEFYQSLVRLFD
                     KGITDAVIFKKVLQKNIDEIWVNKIEQ"
     gene            complement(2897..4507)
                     /locus_tag="PLQ68_10390"
     CDS             complement(2897..4507)
                     /locus_tag="PLQ68_10390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002852337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK73103.1"
                     /translation="MDAFLSWFFEFMNFMLQGIIRFFVGIFEGIVQTFNIGRYIEIFR
                     SHYQQFGFLDWVFSILAIILILAVWLTIILLIVFGVRRIVRLRKNTVTQESIFEEITD
                     LHKEILRLNDEKDRILSLKVSQAGISAKDLAKELSDITEKSYEKIKQMEGVDIPDMEA
                     AEDYAKSQAPMAPEALIESRFSKLIEVDQKYTYYTPEAYYTGFTLEELCKDIRNFACS
                     KMNLYYELEMIRTLIASMASTKLILLQGISGTGKTSLPYCVGKFLKNDATMASVQPSW
                     RDRTELFGYFNEFTKKFNETDVLRRIYESSYNDDINLIVLDEMNIARVEYYFAEMLSI
                     LEMPDPEQWKLELVPSSWPSDPRNLTGGRLMIPENIWYLGTANNDDSTFAVSDKVYDR
                     AFTINFESKGQPFDAPDTEARSISFSYLEELYSDAWEKYPVTKDNLDKIAKLDIYIIQ
                     KFRIAFGNRIMKQLYEFVPVYVACGGTEIDGIDYVLYTKVFRKFESLNLSLIREDLRE
                     LIVYLNKSFGKSNMNECKNYLERLQKMY"
     gene            complement(4539..5216)
                     /locus_tag="PLQ68_10395"
     CDS             complement(4539..5216)
                     /locus_tag="PLQ68_10395"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HXK73104.1"
                     /translation="MKKGLKIVGNILLWLFVVVAVFMTIIAFSSTKNENGVAVIFGRM
                     PITILSESMDPTLKKGDLIISHELSPEQKGTLSEDDIITYKVDLNGDGFMELNTHRIT
                     AVRTNGGFVYYTTKGDNNAIADTQEVRYDNVVGVYNGRRVPAVGAVLNFLQTPTGFLI
                     CVVIPLILFLLYEIYNFIRVMISMKSDKQNKEYEEEIKKKAIEEYLAQQKTEQPDKAV
                     SEDTKTS"
BASE COUNT         1591 a         1012 c          692 g         1979 t
ORIGIN      
        1 tttaacccca ttctgtaatt aaataatata tatactatat atctatacta ttataagttt
       61 tccagatctt tcttcattga acggatttct cttctgagtt tatcaatttc tgcagtatat
      121 ttattaaaat actcaacatc atcatcattt ggagtctcgt gctttaaaat agcttctact
      181 aatgcggatt gagaacgata acgttttttc tccaattcct ctatcaattt gatactgttt
      241 ttaattcctc tttttaattt aattttgtta aacatatgac ccactatttc ctttcttgaa
      301 cgattttcat taataattct tcaagtaacc tgtaacctat atctagtcga ccctcgtgaa
      361 gatttttaaa agcatcatac acttcggacc tataactaac aaaagaatct ggtaactgca
      421 cagcaatata gtaaagttcg cttattacaa actgtctttt ccctgtttcc ccattgtgat
      481 tcattaatgt tctcataaca ttcagttctg aaactacttc agtataaatg acttggttat
      541 ctacataaag aacattatcg tatacatatt ttaaagaagc attgattacc ctgtctagaa
      601 gcatagttga atcatttaat ttgacaaggt aaagcgatag aaagaatata agcattacag
      661 taagaataat cgtggtaatt cttaatgatt tgagttgtct gtttttacgt acctggtcaa
      721 aattataaaa gttttttctc agaaggctct gataatcaga atcatctgta ttttccatat
      781 cttcagaatt cagtatcctt ttaatcagat tcgttttttc cttcttctgt tccggatcct
      841 tcggattctt ttgttgctcc atcattatca gcctcctttc cttcaatctt ttctttttcc
      901 acattctttg ctgttttagc aaggttaatt atttcaaaca caaatagtaa tactgctggt
      961 actacgagga gtataaatat aaaggtcctg ctttgaaaaa ttcggcctgc tttacctaaa
     1021 gtaatgagat tcttctgata tactccgata attgatgatg aatctacctc atatgcatcc
     1081 gaaattgcat tcgcatctcc ttttgtaata aatacaaaat tgccgttttc aattctgatg
     1141 tctgttattc tgtgagtgtt tggcaaccct cttatagtag ggtctttaga atagaaagtt
     1201 ataacatcac caacttttaa ttcagcaggg tcaacttttt ttatcaatat gaatgtattt
     1261 tcaggaattt gcggctccat actggttgat atgatattca ttacactata accaaaaaat
     1321 gatggaactt ttttagttat ctgcgaataa atcatgttac ctacaaatat tataagaaaa
     1381 actaaaagta ctgcaaaaat tgcagtgata atccatttac ttttcttttt gcctttaaat
     1441 tcactcatat aaacctctat caacaatcta cgttgctatt ttttcttgtt ctttattttt
     1501 tcttgtttct taagttcagc tgcatataaa cgttcagatt tcaaagcatt ctcaatggct
     1561 tttcttatct taagttcttc tcttgtcagt tttgtgtttt tcctgcttaa ctgttcaata
     1621 tatacttttt taacattagg gtttaaaaac tcatattcat ctgcaaattc ttctgtatta
     1681 atcttcttaa atcgtgcatt gtacttaatt tttgctttac tttcatttat tatatcatcg
     1741 actttatctg ttgtgttctt acggaagaac acatactgat atgccattag tttatagaag
     1801 ttctcaataa gctctgttga aggcttactg gatgtcgaac tgctttccac ttcaaacccg
     1861 gcactttcat aactttcaat aaactgccac agttcaagac actgccttaa gtgaacattt
     1921 ttcaatattg catttgtcct cattactggc ggtcgtataa atgctcttcc catttgagta
     1981 ataaactgag aagactgata ttgacgtatt atctttctta aacgtaaaac tcttttccat
     2041 aaagatgatt ttgacatatc atcaagaatg ttgacatttt tttcttcaga ttgaccttct
     2101 ttatcatcag cagcatcatt agtctcaata gtaagactta tcttaacttt tgtatcaata
     2161 acttttgtat caatatccaa agacattaca ttggatatct cgtcagctcc gtattctttt
     2221 aatttgtcgt atcttgagtc tatgaaaata cctagtttat aaagcagtgt gtttatgaat
     2281 ttattttcat atgtaaatat atcttcatcc ttgtaaacat ttaaaagttt gttagggatt
     2341 acattgcctt tttcatcaat actgtcaata aaatttgtat gggtagctaa gtgctgtaat
     2401 gatttaactg taatttttct tgtcagttca attggcttaa tctcttcgac ttcctttata
     2461 tctttccttg ttgttctaag tgctgtatca agataaggta aagcctgttc aatcttatta
     2521 acccatatct catcaatatt cttttgtaat acttttttan nnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnnnnnc tgttcaatct tattaaccca tatctcatca atattctttt
     2701 gtaatacttt tttaaagata actgcatctg ttatgccttt atcaaataat ctgactaaag
     2761 attgataaaa ctcataatcc tcatatatat aggatataaa gtcttttgta tcttcgtata
     2821 ttgaattttt ctggtcgtca tcaagcacta gctttttgct ataccttggc ataacgaatt
     2881 cctccttcag aaattatcaa tacatctttt gtaatctttc cagataattc ttgcattcat
     2941 tcatattgct tttaccgaaa ctcttattaa gataaacaat tagttctctt aaatcttctc
     3001 taatcagaga aagattcaaa ctctcgaatt ttctgaaaac ttttgtataa agaacataat
     3061 caataccatc tatctcagta ccgccacaag ccacatatac aggaacaaat tcataaagct
     3121 gcttcataat acgattacca aaagcaattc tgaatttttg gattatatat atgtcaagtt
     3181 tcgcaatctt gtcaagatta tcttttgtta caggatactt ttcccatgca tcggaatata
     3241 actcttcaag atatgaaaag cttattgatc tggcttcggt atctggagca tcaaatggct
     3301 gtccttttga ttcaaaattg atagtaaatg cacggtcata cactttatct gaaactgcaa
     3361 atgttgaatc gtcattattt gctgtaccta aataccatat attctctgga atcattaacc
     3421 ttccgcctgt cagatttctt ggatctgatg gccatgagct tggtacaagt tccagtttcc
     3481 actgttctgg atcgggcatt tcaagtatag atagcatttc ggcaaaatag tactctacac
     3541 gtgctatatt catctcatca agtacgatta gattaatatc atcattatat gatgattcat
     3601 atattcttct taaaacatca gtttcgttaa atttcttggt aaattcatta aaataaccaa
     3661 atagttctgt tctgtctctc caggagggct gaactgaagc catagtcgca tcattcttca
     3721 agaacttgcc tacacaatat ggtaaagatg ttttaccagt accagatatt ccctgtaaaa
     3781 gaatcaattt agttgaggcc atactcgcta tcagtgtcct aatcatttct aattcgtaat
     3841 acagattcat tttactgcat gcaaaattcc taatatcttt acataattct tccagtgtaa
     3901 atccagtgta atatgcttca ggtgtgtaat atgtatattt ctggtctact tctattaatt
     3961 tactgaatcg gctttcaatt aatgcttcag gagccattgg agcctgagat tttgcataat
     4021 cttcagcagc ttccatatca ggaatatcaa caccttccat ctgctttatc ttctcataag
     4081 acttttcagt aatatcggat aattcctttg ctaaatcttt agcagaaata cctgcctgag
     4141 agacttttaa tgatagtatt ctgtcttttt catcattaag cctcaaaatt tctttgtgaa
     4201 gatcagtaat ttcttcaaag atactttcct gagtaacagt gttttttctg agccttacaa
     4261 tccttcgtac gccaaaaaca atcaacaata ttattgttaa ccagacagct aatatcaaaa
     4321 ttatcgcaag aattgagaat acccagtcaa gaaatccaaa ttgctgataa tgacttctga
     4381 atatctctat atacctgcca atattgaaag tttgtactat gccttcaaaa attcctacaa
     4441 agaacctgat aataccctgt agcatgaagt tcataaactc aaaaaaccaa cttaaaaaag
     4501 catccatacc ttatgtttat cctttattca agattaaatc aagacgtttt tgtatcttcg
     4561 gaaacggctt tatctggttg ttcagttttt tgctgagcaa gatattcctc aatagctttt
     4621 ttcttaattt cctcttcata ctccttattt tgcttgtcgg atttcataga tatcataact
     4681 cttataaagt tatatatctc atatagcaag aacagtatta atggtattac tacacaaata
     4741 agaaacccag tcggtgtctg aaggaaattc aaaactgctc caactgctgg aactcttctt
     4801 ccgttatata ctcctactac attgtcatac cgtacttcct gagtatctgc gatagcgttg
     4861 ttatcacctt ttgtcgtgta gtatacaaat cctccatttg ttctcacagc tgtaatcctg
     4921 tgagtgttta actccataaa accatctccg tttaaatcca ctttgtaagt gatgatatcg
     4981 tcctcactca gcgttccctt ttgttcaggt gaaagctcat gactgattat taaatcacct
     5041 ttctttagtg ttggatccat ggattcagat aagattgtaa tgggcattct accaaaaatt
     5101 actgctaccc cgttctcgtt cttagtagat gaaaacgcta ttattgtcat aaacacggct
     5161 actactacaa ataaccataa caatatatta cctactattt ttaacccctt tttcataaaa
     5221 actcctccat tcaaactaca taaatcgcat atctgtactt aacagaaaag gtctttcagt
     5281 caagatgaaa gatcttttcc attaagcaaa ctcagtgcaa tcaactgata atgcttaatg
     5341 ccgacttact aaattatcaa tttccccgcc cgca
//
LOCUS       DAOSXM010000116         5315 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2111
            NODE_10331_length_5315_cov_10.964068, whole genome shotgun
            sequence.
ACCESSION   DAOSXM010000116 DAOSXM010000000
VERSION     DAOSXM010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864178
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5315)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5315)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:24:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,261
            CDSs (total)                      :: 2,229
            Genes (coding)                    :: 2,218
            CDSs (with protein)               :: 2,218
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 3 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5315
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10331_length_5315_cov_10.964068"
                     /isolate="AMR_MDS_2111"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(99..1052)
                     /locus_tag="PLQ68_10400"
     CDS             complement(99..1052)
                     /locus_tag="PLQ68_10400"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: protein motif:HMM:TIGR00747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HXK73105.1"
                     /translation="MSLRITGTGSCLPKKTVSNEELSKIVETSDKWIKERTGISKRQI
                     CVDETITDLAYEAAVQALESSGTKAEDLGYIICSTIGSDHITPSLACAVGERLNSTCP
                     AFDLNAACSGFIYSLDMADSLISTNKADKILIICAEKMSSYTDWTDRSTCVLFGDGAG
                     ACVCEKGESLKYLYTDTLCNTSILQVDAGKGNSPFSDTVPRGYLKMDGKEVFKFAVTA
                     FERHIEKAMKAINLNVSDIDMYLLHQANKRIIDFARTRLNQPDNKFPTNVEKFGNISS
                     ASIPILLDELLRDNQINKGDRLFLSAFGAGLTSGCCVLEWQ"
     gene            complement(1053..>1430)
                     /locus_tag="PLQ68_10405"
     CDS             complement(1053..>1430)
                     /locus_tag="PLQ68_10405"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HXK73106.1"
                     /translation="KSLQSISDIPITVSEAHLLQAVAKNGGQASVSELSGILKVTLPT
                     TTVAVKKLEKKGFLEKGTSSEDGRKSVITLTLLGQKAQRAHNLFHRKMVRNVSSVFNE
                     QEKEILLSSMSKLSDFFKDKAEN"
     assembly_gap    1431..1530
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1531..2555)
                     /locus_tag="PLQ68_10410"
     CDS             complement(<1531..2555)
                     /locus_tag="PLQ68_10410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK73107.1"
                     /translation="MKNNKNVWLLTVILILTLVFTGCVSNKQITYNRNEYAVKDMIGI
                     YGIYSDGDEYLAMGYDRENSYILSGHLGKDDMESVKFEIPYGDDYRGSFQKVYVKNDE
                     IYLINQVYRYRYSTDANAAPYSWNVLLFGYKEGIYIYCVDKEGNIKNANEITEYIDKT
                     DNELLGIEQNVTPKNMFVDNDKNIFVVYEKKVVVFDKDMNYLNEYAEPNTSIIYSVLT
                     DKGQIMLICSNTSSITVMISDSSGVFSEYPLNPYKDASVYPVGVFAYKKNDKLIYMDD
                     EKGIYSLDMKTSEASLMFEWSDEKDRDILVPNPLVANENMLTLMDNGILFVNMDKDSF
                     GRSEKVY"
     gene            complement(2658..3635)
                     /locus_tag="PLQ68_10415"
     CDS             complement(2658..3635)
                     /locus_tag="PLQ68_10415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK73108.1"
                     /translation="MKKNSKFFICILCIMLILTSNVYAYSIDKSNSRICFEDFLFNKL
                     IFERMALLKEINIDEEKLLEIDNALKSIGCTFINDIDAKDKYPEIKAIVPGGTSVNWI
                     ERRISNYSYNGELYNIQKIVATANANSSCLKYTGIQQIFNSNDYTIGNNTLINAVTHY
                     YGGLYLGNTTTLSLLNAITSNIGYSIDSNTTVRTPQTSYSWLMNQTVVFSYVRKENET
                     DDEQVLTHISTGADVSIYSTIMGFQYNNGSWSSPMNISDHVNYVVYPAEFNSTFYAVS
                     YYSAVVGAGGLGIGGPIFDAIFDTSIKGCQNSNVSTIYYCWPSYPSQVF"
     gene            4052..>5315
                     /locus_tag="PLQ68_10420"
     CDS             4052..>5315
                     /locus_tag="PLQ68_10420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009536663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXK73109.1"
                     /translation="MEIKRDIYLNQLKNYAWDGQVKVITGIRRCGKSYLLRTLYKNYL
                     IESGVSENNIICIELDLAKDIRYRNPLELASYIRSKLDGKKEPFYLFIDEIQMSDIVA
                     NPYNEKGKPITFYDTLNDLKSIDNLDVYVTGSNSKMLSSDILTEFRGRYDEIRMHPLS
                     FFEYLAAAGGSKETAFDNYAFYGGMPLVLTRPDDEAKMAYLKSLFSEVYIKDIVERKQ
                     ITRKDILSDILDFLSSVIGSLTNPSKIANTINSMKKQKGEDHVSPNTISSYIGHLEDA
                     FLFTECKRYDVKGKSYFEYPSKYYCEDVGLRNARIGFRQQELNHIMENIIYNELIIRR
                     ANVDIGIVFSNEKTENGEQKRLQREIDFIATKGNKKIYIQSAFAIETESKRISETKSL
                     SLTRDSFPKLLIRNDMRKRWYDDNGVLNI"
BASE COUNT         1688 a          938 c          792 g         1797 t
ORIGIN      
        1 gcaagttttg catcagctat ccatgccatt cctccttgga atattgggta ttcaatgcct
       61 aaaaggtcac ataaatctgt tcttatcata gttatctcct attgccattc cagtacacag
      121 cagccagatg taagcccggc tccaaatgca cttaagaaca gcctgtctcc tttattaatc
      181 tgattatctc taagaagttc gtcaagcagt atcggtattg atgctgatga aatattcccg
      241 aacttttcaa catttgtcgg aaatttgtta tcaggctgat taagccttgt tcttgcaaag
      301 tctattatcc tcttgttagc ttgatgcaga agatacatat caatatcaga tacatttaaa
      361 ttaatagctt tcattgcttt ttcaatgtgt ctttcaaatg ctgttacagc aaacttaaag
      421 acttctttac catccatctt caaatatcct cttggaacag tatcagaaaa cggggaattg
      481 ccttttccgg catccacttg cagtatggaa gtattacata atgtatctgt gtaaagatat
      541 tttaaagact ctcctttttc gcatacgcaa gcgcctgctc cgtctccaaa cagcacgcag
      601 gtgcttctgt ctgtccagtc agtatatgag gacatctttt ctgcacatat tatgagtatc
      661 ttatcagcct tatttgttga aattaaagag tctgccatat ctaaagaata tatgaatccg
      721 gaacatgccg catttaaatc aaatgccgga catgttgaat tcagcctttc tccgacagcg
      781 catgctaaag atggggttat atggtctgag ccgattgtag agcagatgat atatcctaaa
      841 tcctctgcct tcgtacctga agattccaat gcctgcactg cagcttcata agctaaatcc
      901 gttatggttt catccacaca gatttgcctt tttgatatac cagttctttc ttttatccat
      961 ttatctgatg tttccactat ttttgatagt tcttcattac tgaccgtctt ttttggaaga
     1021 cagcttcctg ttccggttat tcttaaactc atttaattct ccgccttgtc cttgaaaaaa
     1081 tctgaaagct tgctcatgga tgaaagaaga atttccttct cctgctcatt aaaaacagag
     1141 cttacattgc gtaccatttt gcgatgaaac agattatggg ctctttgcgc tttctgtcct
     1201 aacagagtca aggttataac ggattttctt ccgtcttctg atgatgtgcc cttttccagg
     1261 aagccttttt tctcaagctt ttttacagca actgttgttg taggcaaagt tactttaagt
     1321 attcctgaaa gctcgcttac actggcctgc cctccgtttt tagctactgc ttgtaaaagg
     1381 tgcgcttcag aaacagttat aggaatatcg cttatgctct gcaaggattt nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn atatatacct tctcgcttct tccaaagctg
     1561 tccttgtcca tattaacaaa taatataccg ttatccatca aagtaagcat attctcattt
     1621 gccactaaag gattgggaac aagaatatct ctgtcttttt catctgacca ttcaaacatc
     1681 aaagaagctt cagatgtttt catatctaat gaataaatac ctttttcatc atccatatat
     1741 atgagtttat catttttctt ataagcaaac actcctactg gatatactga agcatctttg
     1801 tatggattaa gagggtattc agaaaaaacc ccactggaat cagatatcat tacagttatg
     1861 ctgctagtat tactgcatat gagcattatt tgtcctttat cggtcaaaac agaatatatt
     1921 atgctcgtat taggttcagc atattcattt agataattca tatctttatc aaatacaaca
     1981 accttttttt cataaactac aaatatattc ttgtcattat ctacaaacat attcttagga
     2041 gtaacattct gttcaatacc taatagttca ttatctgtct tatcaatata ttccgttatc
     2101 tcattagcat tctttatatt tccttcttta tctacacaat atatgtaaat cccttcttta
     2161 tatccaaaca aaagaacatt ccatgaatat ggagctgcgt ttgcatccgt agagtatcta
     2221 tatctgtata cctgatttat cagatatatt tcatcgtttt ttacgtatac tttttgaaaa
     2281 gaacccctat agtcgtctcc atacggaatc tcaaacttta ctgattccat atcatctttt
     2341 ccaagatgac ctgacagtat atatgaattt tcacgatcat atcccattgc aagatattca
     2401 tcaccatctg aataaattcc atatatccct atcatatctt taacagcata ctcattcctg
     2461 ttgtaagtga tttgcttatt tgaaacgcag cctgtaaaga ctaatgtcaa aatcaaaata
     2521 actgtcaaca accaaacatt tttattgttt ttcatttaga atctcctaac ataatatttc
     2581 ctatacaact ttttgttaca taattattcc atgaaaactt attgtctaca gtagttttca
     2641 tattatctgc attaaaatta aaacacctga gaggggtagg aaggccagca ataatatata
     2701 gttgatacat tagaattttg acatcctttt attgaagtat caaatatagc atcaaatatt
     2761 ggtccaccta taccaagacc gccagcacct actacagctg aataatatga aactgcataa
     2821 aaagtactgt taaattcagc aggatatact acatagttta catgatcact gatattcatc
     2881 ggactactcc atgatccatt attatactga aagcccatga ttgtagagta tattgaaaca
     2941 tcagcacctg ttgatatatg agttaacacc tgctcatcat ctgtttcatt ttcttttcta
     3001 acatatgaaa atacaacagt ttggttcatt aaccatgaat aagacgtttg tggtgttcta
     3061 acagtagtat ttgagtcgat actgtatcca atatttgacg taattgcatt aagtaaactt
     3121 aaagttgttg tattacctaa gtataagcca ccatagtaat gtgttactgc gtttatcaat
     3181 gtgttattac caatagtata atcatttgaa ttaaatattt gctgtattcc agtatatttt
     3241 aaacatgagg aatttgcgtt agctgtagct actatttttt gaatattata caattcccca
     3301 ttatatgagt aatttgatat tctacgctca atccagttta ctgaagtacc tcctggaaca
     3361 atagctttaa tttcaggata tttatctttt gcatcaatgt catttataaa tgtacagcca
     3421 atggatttta aagcattgtc aatttctaac aatttttctt catcaatatt tatttctttc
     3481 aataaagcca tgcgttcaaa tattagctta ttaaataaaa aatcttcaaa acaaatccta
     3541 ctattagatt tgtctattga atatgcatat acattgctag ttaaaattag cataatacat
     3601 aaaatgcata taaaaaattt actatttttt ttcatcacta aaatatcctt cgtttatatg
     3661 ttatggtata taatatacta attatcaaca attgtcaaga atacatgaaa agtttacaaa
     3721 ttcaggaaaa atatgcattt ttctgctaag cttactctta cactatatat aacgtaaaaa
     3781 aggagcaaag ggtggacaac atttaaaaat atttttattc tcatataaaa tattctataa
     3841 agagttcata tttttaattt gcttctgtct atttgtctta atgactgtat gtcatgtaat
     3901 caatttcatg gtaaatatga tatcagattg agaagtagcg catatatatg cttaagtttt
     3961 caatgcaata ttgactataa agataaaggc aggtataatc atcattgaga agtagtgtac
     4021 caatatatgc aagttctcaa tggaggttcc gatggaaatt aaacgcgaca tatatctgaa
     4081 tcaattaaaa aattatgcat gggatgggca agtaaaggtt attaccggaa tccgaagatg
     4141 tgggaaatcg tatcttttac gtacattgta taaaaattat cttatagaat caggtgtcag
     4201 cgagaacaat ataatctgca tagaacttga tcttgctaag gatatccgtt acagaaatcc
     4261 cctggagctt gcatcatata taagaagcaa actggatgga aagaaagaac cattctatct
     4321 atttattgat gagatacaga tgtctgatat agtcgctaat ccgtataacg aaaaaggcaa
     4381 gccaattacc ttctatgata ctcttaatga tttaaaatct attgataatc ttgatgttta
     4441 tgtaacaggc agtaactcaa aaatgctttc aagtgatata ctcactgagt ttcgtggccg
     4501 ctatgatgaa atccgtatgc atcctctctc tttttttgaa tatcttgctg cagcaggcgg
     4561 gtcaaaagaa actgcttttg ataactatgc attttatggc ggtatgcctc ttgtgctaac
     4621 tcgtccagat gatgaagcaa aaatggcata tttaaaatca ttattttctg aagtatatat
     4681 caaggatatt gttgagagga agcaaataac tagaaaagat atattatcag atattttaga
     4741 tttcctgtct tctgttatag gttctcttac aaatccttca aaaatcgcaa atacaataaa
     4801 ctccatgaaa aaacagaaag gtgaggatca tgtctcacca aatactataa gctcctacat
     4861 agggcatttg gaggatgctt ttttgtttac tgaatgtaag cgttatgatg taaaaggtaa
     4921 atcatacttt gaatacccaa gcaaatatta ctgtgaagat gtcgggcttc gtaatgcaag
     4981 aatcggtttc agacaacaag aactgaacca cattatggaa aatattattt acaatgaatt
     5041 aattattcga cgtgcaaatg ttgatatagg tatagtattc tcaaatgaaa aaacagaaaa
     5101 tggcgaacaa aagagattac aaagagaaat tgactttatt gcgacaaaag gcaacaaaaa
     5161 gatatatata cagtctgctt ttgcgataga gacggaatca aagagaatat ctgaaaccaa
     5221 atcattatct cttactcgtg attcttttcc aaagctgctt atccggaatg atatgaggaa
     5281 acgatggtat gatgataacg gtgtgctgaa tataa
//
LOCUS       DAOSXM010000117         5253 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2111
            NODE_10512_length_5253_cov_10.029627, whole genome shotgun
            sequence.
ACCESSION   DAOSXM010000117 DAOSXM010000000
VERSION     DAOSXM010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864178
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5253)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5253)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:24:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,261
            CDSs (total)                      :: 2,229
            Genes (coding)                    :: 2,218
            CDSs (with protein)               :: 2,218
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 3 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5253
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10512_length_5253_cov_10.029627"
                     /isolate="AMR_MDS_2111"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2055
                     /locus_tag="PLQ68_10425"
     CDS             <1..2055
                     /locus_tag="PLQ68_10425"
                     /inference="COORDINATES: protein motif:HMM:NF014502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HXK73110.1"
                     /translation="IMRNWHELGITLGVCYYPEHWPKSIWEDDIDRMLDMGIRFVRIA
                     EFAWTIMEPEESVFDFSLFDEFISLCENKKMKVILGTPTATPPAWLTMKYPQTLNADN
                     SGNPYSHGHRRHYNMNTDIYMKLSARIVEKMANRFKDSNAVIGWQLDNEFNCSISSYY
                     SKADDAAFKKYCKEKYKTLENFNECMGNVFWSQTYTDWEQVHIPINVPKNAFNPHMEL
                     EYRKFISHTVIKYAKMQSDILRSIVPKNQFITTNGIFRNLDYVELSKQALDFMTYDSY
                     PNFAYDMYPQFKRKRTLKDRDWSRNLARVRGISGNFGIMEQQSGANGWNTGMESPMPK
                     PGQIRLWTYQSIAHGADFVSYFRWRTSPVGTEIYWEGIYDQSNTYNRKIEEISRTAKE
                     LDKLKELDNTEYFAKVAYLTDYANECDQDIDAVRSKYQSESDDSWHLATQLTHTPMDY
                     VYMSDNTKAEELKKYDLLIYSHPCILTQKIADTLTEYVTEGGTLVIGARTGFKDEYGR
                     CPMTSVPGFMKNIANVESADHTLIGPLDEQVSATWDNEKIDMPVFNEILEPTSKDTKI
                     LATYDNSYYANMSAVTESIVGKGRVIMVGGAFSTGMAEKILEYTNTLNPYKDLLTLPK
                     ECEIAIRKKDNTEYIFILNYSKDEKTIYLHKECIDLIDQKTVKVTVSIKGYGVILLKP
                     RG"
     gene            complement(2223..2507)
                     /locus_tag="PLQ68_10430"
     CDS             complement(2223..2507)
                     /locus_tag="PLQ68_10430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005813402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HXK73111.1"
                     /translation="MNKASEKLSHMKKFLMKDEEFKAEYEKLKPRYEIISQIIKVRAD
                     NNITQQELALLTGTQKSNISRLESGSYNPSLDFLIKVARSLGKEIKITLV"
     gene            complement(2562..2864)
                     /locus_tag="PLQ68_10435"
     CDS             complement(2562..2864)
                     /locus_tag="PLQ68_10435"
                     /inference="COORDINATES: protein motif:HMM:NF017757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HXK73112.1"
                     /translation="MNWEVEFYRKANGTIPVMNYLQSLNKKIGAKVFSEIELLEKHGI
                     NLREPYVKPIRGKRYKGIYELRIKQSSNILRILYYVCNRRKFILLHGFLKRLMQYL"
     gene            complement(3179..5224)
                     /locus_tag="PLQ68_10440"
     CDS             complement(3179..5224)
                     /locus_tag="PLQ68_10440"
                     /inference="COORDINATES: protein motif:HMM:NF020121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase trimerization
                     domain-containing protein"
                     /protein_id="HXK73113.1"
                     /translation="MEKHIDVRKNPWQSRYGWIWYNSKEILRDTEEDIDNLVKSFADK
                     GINILIGFSCTHFRWNFYRHFDKITECIRRIVKACHKYGILYVEHHSSHLTFNPLNDE
                     DLVYIDNILEKRDSKVSEWPGLIEDATNDFSVDGKMISSFRQISGKSGKWGITTYHGY
                     GMCFNNEDYVERYLKYLESLYAVGIDGIMTDDVQFFGNDGMGWKAFNACTCVHCRNKF
                     REKYGYEIPQPQDWDKFYWNFKDPVFVAWKRFKDESTLEFVYKVKKHYEDLGLNQFRP
                     NYVSSILPTNKTAYPFEKCADVWDCVFQENCTSSVIKESYLSFALEAIHRYNLGRLNK
                     IPSMSMFYPVRADALYFAWALAKSWGQLFTNCGGEGTGSTIDEKWLRDFEIKHSSSYT
                     NPKKISDLAFMLSFDTRDYSDGCETHQENFIRWLQASYLSGIATDMVFENAQYKDFAR
                     HKAIVCAHTVMVSDSVLKKLRRYVKEGGHLITIGDFAKYKPDGSLRDRITLGRKSQQL
                     GDGKVTVLSDDDCYDKYHWVLNISRFETDSVKTSKEVPEYAVEKLRQTGGKALLNAIN
                     NDVIVNVSSEQDIFTSLFKTRSGYALNIVNVQNTITKEGVANHYIPITPYEKGATAIK
                     HDIDIKIHMDIPVKKISLHSPERSRSLNIPYTKQADGTLSFTIPKKKFAGYALVVIK"
BASE COUNT         1666 a          895 c          907 g         1785 t
ORIGIN      
        1 attatgagaa actggcatga attaggtata actctaggag tctgctatta tcctgagcac
       61 tggccgaaaa gcatatggga agatgatata gacaggatgc tggatatggg tatacggttt
      121 gttcgtatag cggaattcgc ctggacaatt atggagcctg aagaatcagt atttgatttc
      181 tcgctttttg atgagtttat atctctttgc gaaaacaaaa agatgaaagt catacttgga
      241 actccgacag ctacacctcc agcatggctg actatgaaat atcctcaaac cttaaatgca
      301 gacaattcag gcaatccata cagtcatggg catcggcgcc actataacat gaacactgat
      361 atatatatga aattatcagc aaggattgtt gaaaagatgg ctaataggtt taaggattca
      421 aacgcagtta tcgggtggca actggacaat gagtttaact gttccatatc ttcctattat
      481 tccaaagcag atgatgcagc cttcaaaaaa tactgtaaag aaaagtataa gacgctagaa
      541 aacttcaatg aatgtatggg aaatgtattc tggagtcaga catatactga ctgggagcag
      601 gttcatatac cgataaatgt tcctaaaaat gcctttaatc ctcatatgga attagaatac
      661 agaaaattca tttctcatac agtaataaag tatgcgaaga tgcagtctga catattaaga
      721 tcaattgttc caaaaaatca gttcataacc acaaatggta tttttagaaa tttagattat
      781 gttgagttat ctaaacaggc tttagatttc atgacatatg attcatatcc gaatttcgca
      841 tatgatatgt atccgcagtt taagagaaag agaactctaa aagaccgtga ctggagccgt
      901 aatcttgcaa gggtcagggg aataagcggg aatttcggta ttatggaaca acagtcagga
      961 gcaaacggat ggaatacagg tatggaatca cctatgccaa aaccagggca gataagactt
     1021 tggacctacc agtcgattgc acatggtgct gactttgtgt catatttcag atggagaacc
     1081 agtcctgtag gtacagagat ttactgggag gggatatatg accagtcgaa tacttataac
     1141 agaaagattg aagaaatatc aagaacagct aaagagctgg ataaattaaa ggaactggat
     1201 aacacagaat attttgctaa agtagcatat cttactgatt atgctaatga atgtgaccag
     1261 gatatagatg ctgtaagaag caaatatcag tcagaatcgg atgattcatg gcatttagca
     1321 actcagctta ctcatactcc aatggattat gtatatatgt cagataatac aaaagctgaa
     1381 gagctgaaaa aatatgacct tttaatatac tcacatcctt gcatacttac acaaaagatt
     1441 gctgatactt tgactgagta tgttaccgaa ggcggcacct tggtaattgg tgcaaggact
     1501 ggttttaagg atgaatatgg aagatgccct atgacatctg ttcctggatt tatgaaaaac
     1561 atagctaatg tggaatcagc tgaccatacg cttattggac cactagatga acaggtgagt
     1621 gcaacatggg ataatgaaaa gatagatatg cctgtattta atgagatttt agagcctact
     1681 agtaaagaca caaagatact tgccacatac gataacagtt attatgctaa catgagtgca
     1741 gttacagaaa gtatagttgg taagggaaga gtaataatgg tgggcggtgc atttagtact
     1801 ggtatggctg aaaagatact tgaatataca aatactctga atccttacaa ggacttgctg
     1861 actttgccaa aagaatgtga gatagcaata aggaaaaagg ataatactga atatatcttc
     1921 atactgaatt attcaaaaga tgagaagacg atttatctac acaaagaatg tatagattta
     1981 attgaccaga agacggttaa ggtgacagta tcaattaaag ggtatggagt tatattatta
     2041 aagcccagag gataattccc ccaggcctta aaatacgact tctctctttt ttgtatctta
     2101 acacaatgtt agtttattgt taagtattaa tttaataata tttgattatt tttatgtaat
     2161 tagttgattt attattgaat ttacctattc aggatgtcaa aactttgtta attaggcaat
     2221 aactagacca aggttatctt tatctctttt cctaatgaac gtgcgacttt aattaaaaaa
     2281 tcaagtgatg gattgtatga tccgctttcc agtctggaga tattagattt ctgtgtccct
     2341 gtgagaagag ctagttcttg ctgtgtgata ttgttgtctg ctcttacttt aataatctgc
     2401 gatattattt cataacgcgg ttttaacttt tcatattcag ctttaaattc ctcatctttc
     2461 ataaggaatt tcttcatatg tgagagtttt tcacttgctt tattcatttt ttgttctcct
     2521 ctcataatca ttcatatatc tcaatgcctg ctcaagctca tttacaggta ttgcatcagt
     2581 ctttttagga atccatgaag taaaataaat tttcgtctgt tacatacata atatagtatt
     2641 cttaggatgt ttgatgattg tttaattctt aattcataaa tccctttata cctttttcct
     2701 cttatgggtt ttacatatgg ttctcttaaa ttaattccat gcttctcgag cagttctatt
     2761 tcagaaaaaa cttttgcacc aattttcttg tttaatgatt gaagatagtt cataacgggg
     2821 atagtaccat ttgctttcct gtaaaattca acttcccaat tcattgggta tctccagtct
     2881 gtaatatatt atcatatatg ataacaataa tcaataatga tgaaacaaaa ctaacactaa
     2941 tttctgtagt aataaagcaa caaagatatg ttttgggaaa tagaatgcaa aaagtgtaat
     3001 atgctacact aattgagttt taactgagag ttgataaaat atattaaatt aaaccagata
     3061 actaaactat ttagaattta tgtaatattc attatatgaa gccagaacaa aaaagaagag
     3121 cttttcagct ccttcttttt aaatctggct ttgtattgtt tatattcaga caagtatctt
     3181 actttataac cactaacgca taacctgcaa atttcttttt aggtattgta aaggacaatg
     3241 tgccgtcagc ttgctttgta taaggtatat ttaatgatct gcttctttca ggagaatgta
     3301 gcgaaatctt cttaactgga atgtccatat gtattttaat gtctatatca tgcttaatgg
     3361 ctgttgctcc tttttcatat ggggttatag ggatatagtg atttgctaca ccttcttttg
     3421 ttatggtatt ttgaacattg actatattca gagcataacc ggaccttgtt ttaaataacg
     3481 aagtgaatat atcctgctca cttgaaacat ttacaataac atcattatta attgcgttta
     3541 gtaaagcttt tccgccggtt tgtcttagtt tttctactgc atattcagga acttcttttg
     3601 atgtttttac tgaatcagtt tcaaatcttg agatatttaa aacccagtga tacttatcat
     3661 agcagtcatc atcagacaaa actgttactt tgccatcacc aagctgttga gattttcttc
     3721 ccaatgttat tctgtctctt aatgaaccgt caggcttgta tttcgcaaag tctcctatag
     3781 ttataaggtg tccgccttct tttacatacc ttctgagttt ttttaatact gagtcagata
     3841 ccataacagt atgtgcgcat acaattgctt tatgtcttgc aaagtcctta tactgagcat
     3901 tttcaaatac catatcagta gctataccag aaaggtaaga tgcttgaagc catcttatga
     3961 agttttcctg atgtgtttca cagccatctg aataatctct tgtgtcaaat gacagcataa
     4021 atgctaaatc agatatcttc tttggattag tatatgatga tgaatgcttt atttcaaaat
     4081 ctcttaacca cttttcatct attgtgctgc ctgtaccttc accaccgcag tttgtaaaca
     4141 gctgacccca tgattttgcc aaagcccatg caaaatacaa agcatctgct cttacagggt
     4201 agaacatgga cattgaaggt atcttgttta atcttcctaa gttgtatctg tgtatagctt
     4261 ctaaagcaaa acttaaataa gattccttaa ttactgaact tgtacagttt tcctggaata
     4321 cgcagtccca cacatcagcg catttttcaa aaggataagc tgtcttgttt gtaggcagta
     4381 tagaagatac gtagtttgga cgaaactggt ttaaacctaa atcttcataa tgcttcttga
     4441 ccttgtatac gaattccaat gtggattcat ctttaaatct cttccaagct acaaatacag
     4501 ggtctttaaa attccagtag aatttatccc agtcttgagg ctgtggtatt tcatagccat
     4561 atttttctct gaatttgttt ctgcaatgaa cacatgtgca tgcattaaat gctttccaac
     4621 ccataccatc atttccaaag aactggacat catctgtcat tatgccgtca attcctactg
     4681 cataaaggga ttcaagatac ttaagatatc tttccacata atcttcattg ttaaagcaca
     4741 ttccatatcc atgatatgta gttatacccc attttcctga ttttcctgaa atctgtctga
     4801 atgatgatat cattttacca tctacagaaa aatcgtttgt ggcgtcttca ataagtcctg
     4861 gccattcaga tacttttgaa tctctttttt ccaggatgtt gtctatatat actaggtctt
     4921 catcatttaa aggattgaat gttaaatggc tgctatgatg ttctacatac aatatgccat
     4981 atttatggca tgcttttacg attcttctta tacattccgt tattttgtca aaatggcgat
     5041 agaagttcca tctgaaatga gtacagctga aacctatcag tatgttaatg cctttatcag
     5101 caaagctttt tactaaattg tctatgtctt cttcagtatc tcttaatatt tcttttgagt
     5161 tataccaaat ccagccatat ctgctctgcc agggattctt tcttacatca atgtgttttt
     5221 ccataattaa ctcctattta aatttcattt act
//
LOCUS       DAOSXM010000118         5184 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2111
            NODE_10711_length_5184_cov_9.181127, whole genome shotgun sequence.
ACCESSION   DAOSXM010000118 DAOSXM010000000
VERSION     DAOSXM010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864178
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5184)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5184)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:24:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,261
            CDSs (total)                      :: 2,229
            Genes (coding)                    :: 2,218
            CDSs (with protein)               :: 2,218
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 3 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5184
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10711_length_5184_cov_9.181127"
                     /isolate="AMR_MDS_2111"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1032)
                     /locus_tag="PLQ68_10445"
     CDS             complement(<1..1032)
                     /locus_tag="PLQ68_10445"
                     /inference="COORDINATES: protein motif:HMM:NF016144.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HXK73114.1"
                     /translation="MGNIIGLKKGIVKLYKHKKQWKKIASDTIVSLYKVFGDTAVDIQ
                     HVGSTSIVHIKAKPIIDIAVAVNNFNDVKPLIPSLENIGVIYRPNVVIAGEMYFVIGD
                     FEHEIFTHHIHVVLSNSKEWINYINFRNYLNAKPAAAKQYEDLKRSLLKKYKYDRMAY
                     TEGKIELINRILKSAFSWSYLDKTVTVTVDRPMGSKHPKFNESVYPVNYGYIDGILAR
                     DNEFFDAYIIGIEKPVSSFTGIVTGVVHRKDDVEDKWVVAPAGSVFNQAEIASYVSFQ
                     EKYFDTYIDSMYQKSCGVIVYRKVNVNIEYLLVKEHESSGWSFPKGHMEAFESETDTA
                     IREVWEETGI"
     gene            complement(1066..2220)
                     /locus_tag="PLQ68_10450"
     CDS             complement(1066..2220)
                     /locus_tag="PLQ68_10450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008726704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HXK73115.1"
                     /translation="MNNRAGYYKTNFPGIAEYKSFVPADLPPVPQLNIDNEMMRLLIQ
                     AHKQLGMLLELSANIPDLNLFIEMYVRKESVLSSQIEGTQATLLDIFDPKINRNTNLD
                     VAEVVNCIKATDYAINALNELPLCNRLIKQTHAVLLDNARGANKSPGEFRSSQNWIGG
                     QGSTLKNAVYIPPELNDMHNAMSELEKFMNEDSELDELVKAALIHYQFETIHPFLDGN
                     GRIGRLLIILYLIDKKILSSPVLYISYYLKKNRTEYYDRMSEVRKNGNYEQWVKFMLK
                     AITQSAQDACSSIVKLAKLHERNLGVIKTMGKSSKNTEKLLTYLEHNPIIAITTASEA
                     LGIAFNTVSEAVRRLIDKDILVIREDFNRNRTFAYKEYLEILTEGTDLYD"
     gene            complement(2412..>3416)
                     /locus_tag="PLQ68_10455"
     CDS             complement(2412..>3416)
                     /locus_tag="PLQ68_10455"
                     /inference="COORDINATES: protein motif:HMM:NF019542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK73116.1"
                     /translation="EPICLHLELEMKWIKEDELKVIQEFGGLKYGKAISRDILIPYDM
                     PLNALHYVIHKLFGWENSHLRNFTIEKKDFKRLIQDKLTNWLFLIGCLLKIPVSYDAL
                     FLREDLYDDWLADIYNGPYKYEEAEELYSTCKNFTDGFKKELQAVLPENKSIEECSFK
                     GLIVDNHSYGNMIIYDNLLESLEVTSVLAQKGQHLNSFEDLGKKMVKRKYQPRSRSLS
                     PEMLPVTHKLIYDYDYGDDWRVYITRPKNFDTLIKSGLITADDFENAKKTVSQLHKPV
                     CLFKDGGPVLDDVGGVYGYYEMLKVIFVNKSKKARTDMLKWAYEQGWELNNIDAPIVL
                     "
     assembly_gap    3417..3516
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3824..5089
                     /locus_tag="PLQ68_10460"
     CDS             3824..5089
                     /locus_tag="PLQ68_10460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003409629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HXK73117.1"
                     /translation="MDMYNCSDMRKIKFTFNYILKFFKWMALASLTGILGGLIGTLFH
                     FIVEKVTNIRTENSWLIYLLPVGGLAIILICRLFKIDSETGTSSIMKALREDKKIPVA
                     IGPVIFLGTAITHLFGGSAGREGAALQLGGSIGTVVGRTFKLDSKDMHIIILCGMSSV
                     FAALFGTPLTAAIFALEVVNAGVLFYSALIPTVVSSLVATRISALFNISPIKFNMQIP
                     EISFASVGEVLLIAAICAGISILFCITIGYSKKFMKTFIKNDYIRIFAGGTLLLLLTI
                     VIRTTDYNGAGMDIIERALSGEARPEAFILKLIFTTITIAAGFRGGEIVPAFFIGSTL
                     GAAIGSFLGVDPGFAAALGLVTLFCGIVNCPIASIFLSIELFGSEGLMLFALACAVSY
                     VLSGSFSIYSEHKLILPKIKDELTNQKDS"
BASE COUNT         1519 a         1022 c          807 g         1736 t
ORIGIN      
        1 tatgcctgtt tcttcccaaa cttcccttat agcggtatca gtctcactct caaaagcttc
       61 catatgtcct ttaggaaagc tccaaccaga actttcgtgt tcttttacca aaagatactc
      121 tatattaacg ttgacctttc tgtagacaat aactccgcat gacttctggt acatgctatc
      181 aatatatgta tcgaagtatt tttcctgaaa agaaacataa ctggctattt ctgcctgatt
      241 gaaaacagat ccagctggtg ccactaccca cttgtcttca acatcatctt ttctgtgaac
      301 aacaccagta actattcctg taaagcttga aacaggtttt tcaatcccta tgatatatgc
      361 atcaaaaaac tcgttatctc ttgctaaaat accgtcaata tatccgtaat taacaggata
      421 tacactctca ttgaatttag gatgtttaga acccataggc ctgtcaactg ttactgttac
      481 tgtcttatct aaatatgacc aggaaaaagc agatttaagt atcctattaa ttaattcgat
      541 cttcccttct gtatatgcca tcctgtcata tttatacttt ttcaaaagac ttctcttcaa
      601 atcctcatac tgctttgctg ctgcaggttt ggcatttaag tagttcctga aattgatata
      661 attaatccac tctttgctat ttgatagaac tacatgtata tgatgggtaa atatttcatg
      721 ttcaaagtct cctattacaa agtacatctc accagcaatt acgacatttg gtctgtatat
      781 gacgcctata ttttctaaag aaggtataag gggcttgaca tcattaaagt tatttactgc
      841 aacagctata tctataattg gcttggcttt tatatgtaca atagaagtgc ttcctacatg
      901 ctgtatgtca acagctgtat caccgaatac tttatataag ctaacaatag tatcagatgc
      961 tatcttcttc cactgcttct tatgcttata tagctttact attccttttt tcagtccgat
     1021 tatatttccc ataatgttgt atattttacc ataataagcg tcttatcagt cataaaggtc
     1081 tgtaccttct gtcagtatct caagatattc tttgtatgca aaagtcctgt tcctgttaaa
     1141 atcctccctt ataaccagaa tatctttgtc tattaatctt cttacagctt cagatactgt
     1201 attaaaagct atacctaagg cttctgatgc agttgtaatc gctataatcg gattatgctc
     1261 aagatatgta agcaatttct cagtattttt tgaactttta cccattgtct taattacacc
     1321 taagtttctt tcatgtaatt ttgcaagctt tacaatagat gaacaagcat cctgcgcact
     1381 ctgcgttatg gcttttaaca tgaatttaac ccactgctca taatttccat tttttcgcac
     1441 ctctgacata cggtcataat actcagtacg gttttttttg agatagtatg agatatatag
     1501 gactggggag gatagtattt tcttgtcaat cagataaagg attatcagta aacgccctat
     1561 cctgccatta ccatctaaaa aaggatgaat tgtctcgaac tgataatgta tcagtgctgc
     1621 ttttacaagt tcatcaagtt cgctgtcttc gttcataaat ttctcaagtt cggacatagc
     1681 attatgcata tcatttagct caggaggtat gtaaactgca ttttttaaag tacttccctg
     1741 tccgcctatc cagttttgcg aagatctgaa ttctcctgga ctcttatttg ctcctcttgc
     1801 attgtcaaga agtactgcat gagtctgctt aataagcctg ttacataatg gaagctcatt
     1861 aagagcatta atagcatagt cagttgcttt aatgcaattt acaacctcag caacatctaa
     1921 gttagtgttt ctatttatct ttggatcaaa aatatccaac aatgtagcct gagttccctc
     1981 aatctgagat gaaagtacgc tttcctttct gacatacatc tcaataaaaa gatttaaatc
     2041 tggtatattt gcagacaact ccaaaagcat cccaagctgc ttatgcgctt gtattaatag
     2101 cctcatcatc tcattatcta tatttaattg tggaactgga ggaagatctg caggaacaaa
     2161 agatttgtat tctgctattc cagggaaatt cgtcttataa tatccggctc tattgttcat
     2221 tttctgtctc caaaaatcaa aaagaaatat agcctcatta tacattccat atttcaatta
     2281 gtcaatccaa attgaaatat ggaactattt tcaccttcct tatttcaatt tcagcaagat
     2341 ttctacttta ctaccaatga aatccttgtt ttggcgcatg cctgttccgc actttggcga
     2401 attagatagt atcacagtac aataggagca tcaatattat tcaattccca gccttgttcg
     2461 tacgcccatt tgagcatgtc agttcttgct tttttgctct tatttacaaa aataacttta
     2521 agcatttcat aatagccgta tactcctccg acatcatcaa gtactggccc tccgtctttg
     2581 aaaagacaga caggcttatg cagttgcgac actgtcttct tggcattttc aaaatcatca
     2641 gctgtaatca accctgattt aatcagtgtg tcaaagtttt taggcctggt tatataaact
     2701 ctccagtcat ctccataatc ataatcgtat ataagtttgt gtgttactgg aagcatttca
     2761 ggtgacaggc ttcttgacct tggctggtat ttccttttta ccattttctt tcccaaatcc
     2821 tcgaaagaat taagatgctg acctttttgg gcaagaacag atgtaacttc aaggctttcc
     2881 aaaaggttat catatattat catattacca tatgagtgat tatctactat cagtcccttg
     2941 aaactacatt cttctataga tttattctct ggtagaactg cttgcagttc ctttttaaaa
     3001 ccatcagtaa agtttttaca tgtactgtaa agctcttcag cttcttcgta cttatatgga
     3061 ccattgtata tatccgcaag ccaatcatca tataaatctt ccctcaaaaa aagggcatca
     3121 taactaacag gtatttttaa gagacaccct atcaaaaaca accagttagt taacttatct
     3181 tgtatgagtc ttttaaaatc ttttttttct attgtaaagt ttcgcaaatg actgttctcc
     3241 caaccgaaaa gtttatgtat cacataatgt aatgcattta aaggcatatc ataaggtata
     3301 aggatatccc tggatatggc tttaccatac ttaaggcctc caaactcctg aattacctta
     3361 agctcatctt cctttatcca cttcatttca agttccaaat gcagacatat cggttcnnnn
     3421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnntctt acacaacttc attatatcta
     3541 ttatgtctag tatataaaaa tacgagctga tgcaaacacc ttctcgctaa aaacattaaa
     3601 agtttttgtt cattgatgcg gtcatacaat ctttttatta ttataatcaa ctactttttt
     3661 tacgaaatca taatatgaaa gtaataactg gttttttata aaaaatgctt cattctaatt
     3721 tcgagtaaca catagaatgc gtgtccacct tcttttcaat acaatataaa ttaaatctga
     3781 tattctgtta tatactgcat attttcttat tgcattccct gttatggata tgtataattg
     3841 ctcggatatg agaaagatta agtttacttt caactatata ttgaagtttt ttaagtggat
     3901 ggcattggcc tcactgactg gaatacttgg aggcctgata ggtactcttt ttcattttat
     3961 cgttgagaag gttaccaata tccgtaccga aaactcctgg cttatatacc ttcttcctgt
     4021 tggcggtttg gcaatcatcc tcatatgcag actatttaag atagattcag aaacaggcac
     4081 atcaagcatt atgaaagcgt taagagaaga taagaaaata cctgtcgcga taggtcctgt
     4141 catattctta ggtactgcga tcacccattt atttggaggc tctgcaggaa gagaaggagc
     4201 ggctttacag cttggaggaa gcataggtac tgtagtagga agaaccttta aactggacag
     4261 taaggatatg catataatca tactttgcgg tatgagcagc gttttcgctg cactgttcgg
     4321 aactccttta acagcagcca tattcgcttt agaagtggta aatgcaggtg tgctgttcta
     4381 ttctgcactc atacccactg tcgtatcttc tcttgttgca acgagaatat ccgctctatt
     4441 caacattagt cctattaaat tcaatatgca gatacccgaa atatcattcg catcagtagg
     4501 agaggtcctt cttatagctg cgatttgcgc aggcataagc atactgttct gtataactat
     4561 aggttattca aagaagttca tgaagacgtt tataaagaat gactatatcc gcatatttgc
     4621 aggcggaact ttattactgc ttttaacaat tgttataagg actacagact acaatggtgc
     4681 gggtatggat ataatcgaaa gagcattaag cggagaagca agacccgaag cattcatact
     4741 aaaactcata ttcacaacca taaccatagc agcaggtttc agaggcggag agatagtacc
     4801 tgccttcttc atcggctcta cccttggagc agcaatagga agctttcttg gtgtagatcc
     4861 tggctttgca gcggctctag gccttgtaac cttgttttgc ggcatagtca actgccctat
     4921 agcttccata ttcctaagta tagaactctt cggttctgaa ggattaatgc tgtttgcgtt
     4981 agcctgtgca gtatcatatg tcctttcagg aagtttcagc atatacagcg aacacaagct
     5041 gatactccca aagataaagg atgagctgac taatcagaag gatagctaac tactaacata
     5101 tttatacaat acagataatt caaccaagaa gataacatag ataataagaa aagattcgac
     5161 tacactctaa ttttatggct aaat
//
LOCUS       DAOSXM010000119         5033 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2111
            NODE_11181_length_5033_cov_9.524910, whole genome shotgun sequence.
ACCESSION   DAOSXM010000119 DAOSXM010000000
VERSION     DAOSXM010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864178
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5033)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5033)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:24:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,261
            CDSs (total)                      :: 2,229
            Genes (coding)                    :: 2,218
            CDSs (with protein)               :: 2,218
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 3 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5033
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11181_length_5033_cov_9.524910"
                     /isolate="AMR_MDS_2111"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            209..1867
                     /locus_tag="PLQ68_10465"
     CDS             209..1867
                     /locus_tag="PLQ68_10465"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF027971.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXK73118.1"
                     /translation="MKIKFYGSAKEVTGSNMILEAAGKKVMIDCGLFQGSSATKAKNF
                     EELKYNVNDIDYVLLTHSHIDHSGRIPLLYKKGYRGNIICTKATKDLCDIMLIDSANI
                     QESDTEYKNHKRNRAGLEPLEPLYTIDDANIALKHFIAVQYHDLIKLDDNISVKFTDS
                     GHMLGSSSITIYVTENKKTTIIGFTSDLGKKNTPIINDPEVIEKCDYLIMESTYGNRQ
                     HKVKSHPEELMFDIIEETINKGGTVIIPSFSVGRTQEMIYAFNKNLAIKKKLSIFDKV
                     PVYIDSPLSTSATEVYKKNAQFFDDEAKQYILSGNNPLDFPNLNFTKSVEDSKFLNRD
                     DSPKIIISSSGMCDAGRIRHHLKHNLYKKKTTVLFVGYQAVGSLGRLLADGAKSVKIF
                     GDTIDVRARIEMIDGFSGHGDMDDLDEYISQIKEKPEKIFLVHGEDDQLYPFAERIKG
                     KFDIDTYIPDSYACFELEKGGKTSELTHDRVLTKNTYEKLTLLELVTDVKRQVKDISE
                     LIAYDEINNLNQQQIETFIKLYSGMRMNIKNLVREVEHNTKVDK"
     gene            1857..3017
                     /locus_tag="PLQ68_10470"
     CDS             1857..3017
                     /locus_tag="PLQ68_10470"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HXK73119.1"
                     /translation="MTNKEIYFDNAASTYIYDEVVQHINKINTEFFANPSSTHLAGIS
                     SFEQIKKMKENFCEILHIKPSSLIFTSGGAESNNFAIKSVAFANMLKSYNIVISSYEH
                     PSVMNTAYYLEKFGFEIRVCHLNEFGHLDLDMLKKLVDNNTILVSTAHVCSETGSVTD
                     IKKISDIIKAINRKCYYHVDAVQSLGKFPINLSMYNNIDLMSFSSHKIHGPKGVGALY
                     IKPKVKIDPFIIGSQSAGLRAGTLNTSGICGFCLALSIIDKNRESNYIKTQILRKYLI
                     DELNSAFSDDIFLVSPMDGSPYILNIAFRSIKSEVLLNALSQKSIYVSSGSACNSKTG
                     ISKTLKTMNIPKDFIEGAIRISFCDLNTTDEVDIFIENLRELIPMLQLFVRR"
     gene            3020..4207
                     /gene="thiI"
                     /locus_tag="PLQ68_10475"
     CDS             3020..4207
                     /gene="thiI"
                     /locus_tag="PLQ68_10475"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004463512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA U4 sulfurtransferase
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HXK73120.1"
                     /translation="MNLSNAEKVLLIKPGEIMLKGDNRQIFEKRLKENLKSVLSDFCS
                     FDFEQESGRYYIIIKNSDYTEYEISHELSKVFGIVAISIAYRIQSNEELLYDFISKFA
                     KKVYDLKPFSTFRISTKRADKTFATQSVDVSKKAGGSVLSVIESLKVDLVHPEITFNI
                     EVRKKHIYVYYEQIQANGGMPVGSAGKAILMLSGGIDSPVAGYMMAKRGLAQEAVYFH
                     SEPYTSEKAKEKVIDLAKTIKPYTLLKRIHVVHFTKAQLAIYEKCPHDELTIIMRRIM
                     MYITNKIASITNCQAIITGESLGQVASQTLESLTVTSQAAEYIVFRPLIGMDKTEIVE
                     IARKIETFETSILPYEDCCTIFVARHPKTRPVLSKIEESEKLLNVNELVKDCIDNLEV
                     IEI"
     gene            4204..4902
                     /gene="lepB"
                     /locus_tag="PLQ68_10480"
     CDS             4204..4902
                     /gene="lepB"
                     /locus_tag="PLQ68_10480"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HXK73121.1"
                     /translation="MKTSSEYETQRDFNEYIENDDYTLNSISRNIPKKDKRKYILKET
                     LLWARALLFVIVIVVVVRGFIGEPVRVIGPSMENTLKEGDVLIMSKISLEIKGIQRND
                     IVVIEIEPVTFDNLTFLNDIVWFRRLFPAHSREDYIKRVIAFENEEISLIDGIVYING
                     KRHDEPYLKDLGSTFEKLVDMPYIVPKGHIFVMGDNRAISKDSRSIGPVSAESVIGKI
                     VFRVWPLNKIGSTN"
BASE COUNT         1877 a          672 c          874 g         1610 t
ORIGIN      
        1 ctcagctgga tagagcaacg gacttctaat ccgtaggtcg ggggttcgaa tcccttctgg
       61 cacgccaaga aatttcccat attacaggga ttttttttgt atatacaaat agttaaactc
      121 ctaaatacta cttttttatg ataaatgaac taatcaatct tacttacaac catttttatg
      181 ttattatata gtttgaaagg attttgatat gaaaattaaa ttttatggtt ctgctaaaga
      241 agtaactggc tccaatatga tacttgaagc cgcagggaaa aaggtaatga tagattgtgg
      301 attatttcaa ggttcatctg ctactaaagc aaaaaacttt gaagaattaa aatacaatgt
      361 aaacgatatc gattatgtgc ttctgacaca ttcgcatata gatcactcag gaagaattcc
      421 actactttat aaaaaaggat acagaggtaa tattatttgc acaaaagcga ctaaggattt
      481 atgcgatatt atgcttatag attctgcaaa catccaggag tcagataccg agtacaaaaa
      541 tcacaaacgc aatcgtgcag gtcttgagcc tcttgaacct ctttatacca ttgatgatgc
      601 taatatagct ttaaaacatt ttatagctgt acagtatcat gatttaataa agttagacga
      661 caatatatca gttaaattta cagattcagg acatatgctg ggatcttcat caatcacaat
      721 atatgtaacc gaaaacaaga aaactacaat aataggattc acttctgatt taggtaaaaa
      781 gaatacccca ataataaatg atcctgaagt tattgaaaaa tgcgactatc ttattatgga
      841 gtcaacatac ggaaacagac agcataaagt taaatcacat cctgaagagc ttatgtttga
      901 cataattgaa gaaacaataa ataaaggagg aactgttatc ataccttctt tttctgtagg
      961 aagaacacag gaaatgattt atgcattcaa caaaaattta gctattaaga aaaaactttc
     1021 tatttttgat aaagttcctg tatatataga cagtccttta tcaacttctg caactgaagt
     1081 atataaaaaa aatgcgcagt tttttgatga tgaagcaaaa caatatatac taagcggtaa
     1141 taatccgctt gattttccaa acctgaattt tactaagtct gttgaagatt caaagttttt
     1201 gaatcgtgat gattctccta aaataataat atcatccagt ggaatgtgtg atgctggccg
     1261 tattagacac catttaaaac ataatttata taagaaaaaa acaacagttt tattcgtagg
     1321 ttatcaggct gttggttctt taggaagact tcttgctgat ggagcaaaaa gtgttaagat
     1381 ttttggcgat acaattgatg tcagagcaag gattgagatg attgatggat tttctggcca
     1441 tggtgatatg gatgatcttg atgaatatat ctcacagata aaagaaaaac ctgaaaaaat
     1501 attcttagta catggtgaag acgatcagtt atatcctttt gcggaacgaa ttaaggggaa
     1561 attcgatatc gatacatata taccagatag ttatgcatgt tttgaattag aaaaaggcgg
     1621 taaaacttct gaattaaccc atgacagagt attgaccaaa aacacctacg aaaaacttac
     1681 tttgcttgaa ctggtgactg atgttaaacg acaggttaaa gatatttctg aacttatagc
     1741 ttatgatgaa attaataatc ttaatcaaca acagatagaa acatttatta aactttactc
     1801 tggtatgaga atgaatatta aaaatcttgt tagagaagtg gagcacaata caaaagttga
     1861 caaataaaga aatatatttt gacaatgcag cttcaaccta tatttatgat gaagtagtgc
     1921 aacatattaa taaaattaat actgaattct tcgcaaatcc ttcgagtaca catctagcag
     1981 gtatttcttc atttgaacag ataaaaaaaa tgaaggaaaa tttttgtgaa attctacata
     2041 ttaaaccaag ttcgttgatt ttcacatcag gtggtgcaga atctaataat ttcgcaatta
     2101 aatctgttgc gtttgcaaat atgttaaaat catacaatat tgtaatatca agttatgagc
     2161 atccttctgt tatgaataca gcttattatt tggaaaaatt tggttttgaa ataagagttt
     2221 gccatctaaa tgaatttggt catttagatt tagatatgtt aaaaaaactt gtagacaata
     2281 acacaatact tgtgagtact gctcatgtat gtagtgaaac aggttcagtt actgacatca
     2341 aaaagatatc agacattata aaagcaatta atagaaaatg ctattatcac gttgacgcag
     2401 ttcagtcatt aggtaaattt cctattaatt taagtatgta taataatatt gatttgatgt
     2461 cttttagcag tcataagatt catggtccaa aaggggtagg tgctttatat attaaaccaa
     2521 aagttaagat tgatccattc ataatcggtt cacaaagtgc aggccttaga gcaggaactt
     2581 taaacacctc tggaatatgt ggattttgct tagcattatc tataatagat aaaaacagag
     2641 aatcgaatta cataaaaact caaattttaa gaaaatatct tattgatgaa ttaaatagtg
     2701 ctttttctga tgacatattt cttgtttcac cgatggatgg cagtccatat atactaaata
     2761 tcgcatttag gagcataaaa tctgaagttc ttctaaatgc attatctcaa aaaagcatat
     2821 atgtttctag cggaagtgca tgtaattcaa aaactggcat atctaaaaca ctaaaaacta
     2881 tgaatatacc aaaagacttt attgaaggag ctataaggat aagtttttgt gatcttaata
     2941 caactgatga agttgatatt tttatagaaa acttaagaga attaattcct atgctccaat
     3001 tatttgtgag gagataaaaa tgaatttatc aaatgcagaa aaagtattgc ttattaaacc
     3061 tggagaaata atgctcaaag gcgataacag acagattttt gaaaaaagac ttaaggaaaa
     3121 tcttaaaagt gttttatctg atttttgttc ttttgatttt gagcaggagt caggaagata
     3181 ctatataatt attaaaaatt cagattatac tgaatacgaa atatcacatg aattatctaa
     3241 agtttttgga attgtagcta tatctatagc atataggata caaagtaatg aagagctctt
     3301 atatgatttt atttcgaagt ttgcaaaaaa agtatatgat ttaaaaccat tcagtacttt
     3361 tagaatttct acaaaaagag cagataaaac ttttgctact cagtctgtag atgtatcgaa
     3421 gaaagcagga ggaagcgttt tgtcagtaat tgaaagtttg aaagttgatt tagttcatcc
     3481 tgaaattaca tttaatattg aagtaagaaa aaaacatata tatgtgtatt acgaacaaat
     3541 ccaagctaat gggggtatgc ctgtaggttc tgctggtaaa gcaatactaa tgttatcagg
     3601 tggtatagat agccctgttg ctggatatat gatggctaaa agggggttag ctcaagaagc
     3661 agtatatttt catagtgagc catacacatc agaaaaagcc aaagaaaaag ttattgacct
     3721 agctaaaaca ataaaacctt atacattact aaaacgaatt catgttgttc actttacaaa
     3781 agcgcagcta gcaatatatg agaaatgccc tcatgatgaa cttactatta ttatgcgaag
     3841 aataatgatg tacataacta ataagattgc aagtataact aattgtcaag ctataattac
     3901 tggtgagtct ttaggacagg ttgcaagtca gactcttgaa agtttgactg taacttcgca
     3961 agctgctgaa tatatagtat tcagaccgct tatagggatg gataaaacgg aaattgttga
     4021 aattgctaga aaaattgaga cttttgaaac atctatattg ccatatgaag attgttgtac
     4081 tatatttgta gcaaggcatc ctaaaacaag accggtttta tctaaaattg aggaatcaga
     4141 aaaattgctg aatgtaaacg aactggttaa agattgtatt gataatttag aggttattga
     4201 gatatgaaaa cttcaagcga atatgaaacg caaagggatt ttaatgaata tatagaaaat
     4261 gatgactata cacttaattc tataagtaga aatataccca aaaaagacaa gcgtaaatat
     4321 attcttaaag aaacattact gtgggcaaga gctttgttat ttgtaattgt tatagttgta
     4381 gttgtaagag gatttattgg agaaccggta agagtaatag gaccatctat ggaaaacact
     4441 ttaaaagaag gtgatgtcct tataatgagc aaaatcagtc ttgaaattaa aggtattcaa
     4501 agaaatgata tcgtggttat tgaaattgaa cctgttactt ttgataatct tacatttcta
     4561 aatgatattg tatggtttag aagacttttt ccagcacata gcagggaaga ctatataaag
     4621 agagtaattg catttgaaaa tgaagaaata agccttatag atggaatagt atatataaac
     4681 ggaaaaagac atgatgaacc ttatttaaag gacctaggtt caacttttga aaaattagta
     4741 gatatgcctt atatcgttcc taaaggacat atatttgtta tgggtgataa cagagctata
     4801 agtaaagaca gcagatcgat tggtccggta tcagctgaat ctgttattgg taaaatcgta
     4861 ttcagagtat ggcctttaaa taaaattgga tcgaccaact agaatatcac ttgtattact
     4921 tatttgctaa tattctcaca tatatgtaaa ataatcatgt tcttagtcag atttgaaaat
     4981 cgttgagttt ccgtgttttg tattaggaag cgattatttg ccttcaattt tat
//
LOCUS       DAOSXM010000120         4976 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2111
            NODE_11378_length_4976_cov_10.796383, whole genome shotgun
            sequence.
ACCESSION   DAOSXM010000120 DAOSXM010000000
VERSION     DAOSXM010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864178
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4976)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4976)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:24:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,261
            CDSs (total)                      :: 2,229
            Genes (coding)                    :: 2,218
            CDSs (with protein)               :: 2,218
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 3 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4976
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11378_length_4976_cov_10.796383"
                     /isolate="AMR_MDS_2111"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(114..971)
                     /locus_tag="PLQ68_10485"
     CDS             complement(114..971)
                     /locus_tag="PLQ68_10485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003486784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HXK73122.1"
                     /translation="MDWLDRMNKAIEYIEDNLTEKISYEKASKIAYCSVFHFQRMFCY
                     IAGVSLSEYIRRRKMTLAAFDLQSSEELIIDISFKYGYESPTAFNRAFQKIHGVSPSY
                     AKKSGTELKAYPRISFTISIKGEQEMNYRIVKKEAFRTVGVKEHYNMNVEEAFSKVPL
                     FWQKTIMEGKMTGILSMMNKEPYGMLGISTCMNGKDFDYYIAVASDLPVSSGMVEYVV
                     PATTWAVFECTGPMPDAIQALQKQIVTEWLPVSGYEYANAPDIEVYYEGDQQSKDYKS
                     EVWLPIVKK"
     gene            complement(1052..1723)
                     /locus_tag="PLQ68_10490"
     CDS             complement(1052..1723)
                     /locus_tag="PLQ68_10490"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HXK73123.1"
                     /translation="MTFRLKDSPLFYEMAQEEIDACVKCSKAKILDFAKDEMIFTADA
                     EPKSVFVLLEGDVLIYKVTLSGKRDIFMHVDKPGDVFGEVYLFMKKARYEYYSVTLKQ
                     SKVIEIPQDYFYYTCSDNCNHHVKLIRNMMRILARKAYLFNLKLQILASGTLRQKIIR
                     YLFEYLEEGIVKMTMSREMFADYLNVARPSLSRELMKMESEGLIIIKGRTIEIPNPNI
                     LDEGL"
     gene            complement(1783..2562)
                     /gene="pflA"
                     /locus_tag="PLQ68_10495"
     CDS             complement(1783..2562)
                     /gene="pflA"
                     /locus_tag="PLQ68_10495"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate-lyase-activating protein"
                     /protein_id="HXK73124.1"
                     /translation="MSVKGWIHSVESFGTVDGPGIRYVLFVQGCPLKCLYCHNPDTWS
                     VYDDKQKNKAGSDCVMKSGKLTDSETIVNDILSYKGYIKTGGVTISGGEPLMQPEFVL
                     DIVKRCKENGIHTAIDTSGCIAVSRSKAAIDEADLILLDIKSLDNEQAIKLTGSSNAN
                     TLATLDYCEKINKKVWIRHVLLPGWTLDSRRLKALAEYLKAYKCVEIVELLPFHKMGE
                     FKWKELGLNYTLSETKEPSLKEIEQAKNIFIKEGISVTLPG"
     gene            complement(2623..4836)
                     /gene="pflB"
                     /locus_tag="PLQ68_10500"
     CDS             complement(2623..4836)
                     /gene="pflB"
                     /locus_tag="PLQ68_10500"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015557746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HXK73125.1"
                     /translation="MDFNNGKWNSEIDTRDFIVNNYSPYDGDESFLEPATDNTKKLWK
                     KVENLLKEERSRGGIYDIDESVISTITSHKSGYIDKDLEQIVGLQTDSPLKRSIMPFG
                     GIRLVYNQLDAYDKKLPEDIGNVFKYRKTHNDGVFDAYTDEMKKARHVGIITGLPDAY
                     GRGRIIGDYRRVALYGTDFLIAQKKKARSEYVFDVMDEKVIRQREEISEQIRALSELT
                     EMAASYGFDITKPATDTKEAIQWLYFGYLGAIKEQNGAAMSLGRVSTFLDIYAEKDLM
                     NGKYTESEIQELVDNFIIKLRMVRFLRTPEYDELFSGDPVWVTEAIGGVCEDGRHMVT
                     KMSYRFLHSLVNLGPAPEPNLTILWSQQLPQNFKNYVASISIKTSSIQYENDDIMRPI
                     YGDDYGIACCVSAMRIGKQMQFFGARVNLGKALLYAINGGKDEMYGTQITPAFEPVKS
                     EYLDYNEVMEKYDYVCEWLAKLYINTLNVIHYMHDKYCYERIEMALHDEEVIRTMACG
                     IAGLSVVTDSLSAIKHTKVRVIRNEEGLAVDFELEDDYPKFGNNNPAVDEIAANVVRN
                     FMDKLRDHKTYREARPTMSVLTITSNVVYGKKTGSTPDGRKAGEPFAPGANPLHGRDS
                     HGSVASMRSVAYLPYEHAEDGISYTFSIVPDALGKTEQIKKNNLSALLDGYFADSGHH
                     INVNVLNKEVLLDAMEHPEQYPQLTIRVSGYAVNFIKLTREQQLDVIYRTYHEKL"
BASE COUNT         1457 a         1044 c          705 g         1770 t
ORIGIN      
        1 attatagaca tatagtatat atatatttac ctcttgtcaa gtatatttaa catttattaa
       61 gaataaaata acctcagtta ctgaggttat taaaaatttc tgtggttata ttattatttt
      121 ttcactattg gaagccaaac ttcggattta taatctttcg actgttgatc tccttcataa
      181 tatacttcta tatcaggtgc atttgcgtat tcgtatcctg ataccggcag ccattccgta
      241 acaatttgtt tttgaagtgc ttgtatagca tccggcatgg gtccagtaca ttcaaataca
      301 gcccatgtag tagcaggaac aacatattca accatacctg agctgacagg taaatcagaa
      361 gctacagcaa tatagtagtc aaaatccttt ccattcatac aggtacttat tcctaacatt
      421 ccatatggct ccttgttcat cattgataaa attcctgtca tctttccttc cataatagtc
      481 ttttgccaaa aaagaggcac tttactgaat gcttcctcaa cattcatatt ataatgctct
      541 tttaccccga ctgttctgaa agcttctttt ttcacaatcc tgtaattcat ttcttgttct
      601 cctttaattg aaatcgtgaa gcttattcta ggataggctt taagctcagt accggatttt
      661 ttcgcataag aaggtgaaac tccatgtatt ttctgaaatg ctctgttgaa agctgtcgga
      721 gattcatacc catatttaaa ggatatatca attattaact cttctgaaga ttgaagatcg
      781 aaagctgcta aggtcatctt tcttctgcgt atatattctg ataaagaaac tcctgctata
      841 taacagaaca tcctttgaaa atgaaatact gaacagtatg caatttttga agctttttca
      901 taactgattt tttcagtcag attatcctct atgtattcaa ttgctttatt cattctatcc
      961 agccaatcca taactacctc acgtcaatca aagtatatat ctttatattt tctcattcct
     1021 ctctttttat gcacaaaaaa gagaagtcaa attacagtcc ttcatcaaga atattgggat
     1081 ttggtatttc aattgttcta ccttttataa ttattaggcc ttcgctttcc atcttcatca
     1141 gttctctgga taatgaaggt cttgctacat tgagataatc agcaaacatt tccctgctca
     1201 tagtcatctt aacaataccc tcttctagat attcaaaaag gtacctgata atcttctgtc
     1261 ttaaagtccc gcttgccaga atctgaagct ttaaattaaa caaataagct ttgcgtgcca
     1321 gtattctcat catgttcctt ataagtttga catgatgatt acagttatct gaacaagtat
     1381 agtaaaagta gtcctgaggt atctctatta cctttgactg tttcaatgtt acagaatagt
     1441 attcatatct tgcttttttc atgaaaagat atacttcacc aaagacatca cccggtttat
     1501 ctacatgcat aaagatatcc ctcttgcctg acaaagtaac cttgtaaatc aagacatccc
     1561 cttcaagaag cacaaacact gatttaggtt ctgcatctgc agtaaatatc atctcatctt
     1621 ttgcaaaatc aagaattttt gcttttgagc attttacaca ggcatcaatc tcttcctgtg
     1681 ccatttcata aaaaagcgga ctgtctttta atctaaaagt catctttact ccttttattc
     1741 atatgttaat tatatatatt gcgaagtaaa attcaaatta tattaacctg gaagggtaac
     1801 agaaatacct tcttttatga atatattttt tgcttgttca atttctttta aactaggctc
     1861 ttttgtttct gacaaagtat aattaagacc aagttccttc catttaaact cacccatctt
     1921 atgaaaaggt aaaagctcaa ctatttccac acatttatat gcttttagat attcagctaa
     1981 tgctttcagt cttcttgaat ccaaagtcca tccaggcagt aaaacatgtc gtatccatac
     2041 tttcttattt atcttttcac agtaatccaa agtagctaat gtatttgcat tactgcttcc
     2101 agtaagcttt atagcctgtt cattatccaa gctctttata tccagtaaaa taaggtcagc
     2161 ttcatcaata gctgcttttg aacgtgaaac agcaatacat cctgaggtgt ctatagcagt
     2221 atgtatcccg ttttctttac acctttttac tatatctaaa acaaactcag gctgcatcag
     2281 aggttcccct cctgatattg taacaccacc tgtctttata tatcctttgt aacttaagat
     2341 atcattaaca attgtttctg aatctgtaag ttttccagac ttcattacac agtctgagcc
     2401 cgccttattc ttctgtttat catcataaac actccatgta tcaggattat ggcagtaaag
     2461 acattttaat ggacaacctt gaacaaacag gacatatctt atgcccggtc cgtccactgt
     2521 tccgaatgat tctactgaat gtatccagcc tttaacgctc atatccattc ctttcctgat
     2581 ttaagtataa tttcaatata tcagcataat atttatatga ttctataatt tttcatggta
     2641 tgtacggtat attacatcca actgctgctc acgagtgagt tttatgaaat tcactgcata
     2701 tccgctaaca cggattgtaa gctgaggata ttgttccgga tgctccatag catctaaaag
     2761 tacttctttg tttaacacat tcacattaat atgatgtccg gaatctgcaa aatatccatc
     2821 aagcaatgca gataagttat ttttctttat ttgctctgtc ttgcctaatg catcaggaac
     2881 tatgctgaat gtgtatgata ttccatcttc agcatgttca tatggcagat atgcgacact
     2941 tctcatactt gctacacttc catggctgtc acggccatgt aacggattag caccaggagc
     3001 gaaaggttct cctgctttac gtccgtcagg ggtagatccg gtttttttac cgtaaaccac
     3061 atttgaagta atagtcagaa ccgacattgt gggtcttgct tctctgtatg ttttgtgatc
     3121 tcttagctta tccataaagt ttctaactac attagctgct atctcatcaa ctgcaggatt
     3181 attatttcca aatttcggat aatcatcttc aagttcaaaa tctacagcta atccttcttc
     3241 attacggatt acgcgtactt ttgtatgctt tatagccgat aaactgtcag taacaacgct
     3301 taaccctgca ataccgcaag ccatcgtcct tataacttct tcatcatgaa gtgccatttc
     3361 aattctttca tagcagtatt tgtcatgcat atagtgaatg acattgagtg tgttaatgta
     3421 cagctttgca agccactcgc aaacatagtc atacttttcc ataacttcat tgtaatcaag
     3481 atattcagac ttgacaggtt caaacgctgg ggttatctgt gtgccataca tttcatcttt
     3541 gccgccattt atagcatata gtaaagcttt acctaaattt actcttgctc cgaagaactg
     3601 catctgtttt cctattctca ttgcagacac gcagcatgct ataccgtaat catctccata
     3661 tataggtctc atgatatcat cattctcata ctgtatagat gaagtcttaa tagatattga
     3721 agctacataa tttttaaagt tctgcggtag ttgctgactc cacagaattg tcagattagg
     3781 ttcaggtgct ggacctaagt ttacaagaga atgaaggaat ctataactca tctttgtaac
     3841 catatgtctt ccgtcttcac atactcctcc aatggcttct gttacccata ccggatcgcc
     3901 gctaaacagt tcgtcatatt caggagtcct caaaaagcga accatacgaa gtttaattat
     3961 aaaattatct accagttcct gtatttctga ttcagtatat ttaccattca ttaaatcctt
     4021 ttctgcatat atatctagga atgtagatac tcttccaaga ctcatagccg ctccgttctg
     4081 ctcttttatt gccccaagat aaccgaaata cagccactgt attgcttctt tcgtatcagt
     4141 tgctggtttt gttatatcaa atccgtatga agctgccatt tctgtcagtt cagataatgc
     4201 acgtatctgc tcgcttatct cttctctttg ccttataacc ttttcatcca tcacatcaaa
     4261 gacatactca gatctagctt tctttttctg agcaataaga aagtctgtac catacagagc
     4321 aactcttctg taatctccaa ttatccttcc acgtccataa gcatcaggca accctgttat
     4381 gataccgaca tggcgggctt ttttcatttc atccgtatat gcatcaaata ctccgtcatt
     4441 atgtgttttc ctgtatttaa acacgttacc tatatcttca ggtagttttt tatcatatgc
     4501 atcaagctga ttgtaaacaa gacgaattcc accaaaaggc ataatagaac gttttaaagg
     4561 cgaatcagtc tgcaaaccga ctatctgttc taaatctttg tctatatatc cagatttatg
     4621 tgatgttatc gtagaaatca ctgattcatc tatatcatat attcctccac gagaacgttc
     4681 ctcttttaga agattttcta ccttcttcca taacttcttt gtgttatctg tagcaggctc
     4741 aagaaaactt tcgtcaccat catatggact atagttattt actataaagt ctcttgtatc
     4801 tatttcgctg ttccatttcc cattattaaa atccatatct atatcctcca taaggttaaa
     4861 atcaattagt tctcagcaaa ctgacattac tgatgcaaat agaatatacc atagaaagca
     4921 tacataatcg gtaacatttg ttacatatgt ttaaatattt aacatttttg tgttaa
//
LOCUS       DAOSXM010000121         4926 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2111
            NODE_11537_length_4926_cov_11.542394, whole genome shotgun
            sequence.
ACCESSION   DAOSXM010000121 DAOSXM010000000
VERSION     DAOSXM010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864178
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4926)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4926)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:24:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,261
            CDSs (total)                      :: 2,229
            Genes (coding)                    :: 2,218
            CDSs (with protein)               :: 2,218
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 3 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4926
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11537_length_4926_cov_11.542394"
                     /isolate="AMR_MDS_2111"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            51..947
                     /locus_tag="PLQ68_10505"
     CDS             51..947
                     /locus_tag="PLQ68_10505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009003769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HXK73126.1"
                     /translation="MSNHDINAKVEIFRPKDVKGVIKDYALITLGVLFVTIGVYFFKF
                     PNNFAIGGVTGLAMILSNALGGTVSSGTIVLVVNVILLIIGYLVLGKKFGNRTLYGSL
                     LFSLSLNLLEILVPMTGPITDEPLLELAFAVMFPALGSAILFNIGASTGGTDIVAMLL
                     KKYTSWDIGRSLLLTDLFLTLAGFTVFSVKTVLLSILGLLIKSTLVDSIIENLNLNKY
                     FTIITKHPDEICEYILKHIHRSATVFDGKGAFSHEDKKIIITVMNRAQAVHLRLYIKK
                     VDPEAFILITNTSEIIGKGFRA"
     gene            981..2312
                     /locus_tag="PLQ68_10510"
     CDS             981..2312
                     /locus_tag="PLQ68_10510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010348138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK73127.1"
                     /translation="MVRTIDFNFSPDNDARTNTTALQEAVDRTGTITIEEPGIYDIDD
                     SIVIPSDTALVFGKGVYIRRTTDTGYVFINKGAYTREYDHNISITGLNLLCNGHNSKQ
                     GNLITGLRGQVCFFYIKDLVLRDLLCLDLMEYSFCIQVCTFENLLIENVHIEGLKDGI
                     HIDKGCKFAIRHGIFKTFDDPIAINAHDYTTGSPEMGWIEDGIIEDCYDLEQEKTVGY
                     FCRVLAGSWLDWYKGMLVQQSDTVVYNNRMYRVNMEPNDIFYTSNTPPSHEEGTKEYD
                     GIKWTMTQDKGIVYNCGCKNIHFRYIYLKKKRHTAFSLTFDKTKYSRGCYPNSIPPVQ
                     EDITFDHIYVQNEITDLIGIRTPVNTIKLTNSAIGNNRINLRNVEEEGITYIPAHILI
                     SNVTFKGDGKQSLVYAAEGRKATLKINGSIKENSTYEPVIEGDIDVLEKDI"
     gene            complement(2493..3449)
                     /locus_tag="PLQ68_10515"
     CDS             complement(2493..3449)
                     /locus_tag="PLQ68_10515"
                     /inference="COORDINATES: protein motif:HMM:NF025124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXK73128.1"
                     /translation="MTTEYKSDYIYEFEATKSIIQDLSFNEAMRYFTERKVEFKEEQK
                     RSLGIKNSDGLYTNLGLIFSDQCIHTIKLAIFQGINKLTFKDRKEFSGSILRQIDDVF
                     KYIELFNRIRSEFSGLDRIDIPDYPVEAVREALLNAITHRDYSYSSSTLISLFDDRIE
                     FVSLGGLPKGITYNDMMLGISVLRNSRICDIFYRLHLIEAYGTGIQKIMESYDDFYIK
                     PKIEVSDNAFKITLPNINYKHEFIIKENAISYNEQKVLDYLKDKYYSTRKEIENYSGL
                     SQATTVRVLANLLEKGLIIRQGEGKSTTYSEKHGLYPSKNTE"
     gene            complement(3582..4496)
                     /locus_tag="PLQ68_10520"
                     /pseudo
     CDS             complement(3582..4496)
                     /locus_tag="PLQ68_10520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009658515.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
BASE COUNT         1640 a          793 c          867 g         1626 t
ORIGIN      
        1 aatatattag tattgactat ttgattttgc agactgataa tttacttgta atgagtaatc
       61 atgatattaa tgcaaaagtt gagattttca gaccaaaaga tgttaaaggg gtaattaaag
      121 attatgccct tataaccctt ggggttttgt ttgtcacaat aggcgtttac ttcttcaagt
      181 ttcccaacaa cttcgctata ggcggtgtaa caggacttgc tatgatacta agcaatgcct
      241 taggtggaac tgtaagttca gggactatag tacttgttgt taatgtaata cttcttatca
      301 tcggatatct tgtactggga aagaaattcg gaaacagaac actgtatgga agcctgcttt
      361 tttcattatc tttaaatctt ctggaaatat tggtgccaat gacaggaccc attactgatg
      421 agcccttatt ggagctggct tttgctgtca tgtttcctgc tttaggttca gcaattcttt
      481 ttaatattgg agcatcaaca ggcggtacgg atattgtagc tatgcttttg aaaaaatata
      541 ccagctggga tatagggagg tctctgcttc tgactgatct ttttcttacc cttgcaggat
      601 ttacagtgtt cagcgtgaag acagttcttt tatcaatatt aggtctttta ataaaatcca
      661 cgctcgttga cagcataata gagaatctta atctgaataa gtacttcaca atcataacca
      721 agcatcctga tgagatatgc gaatatatct taaagcatat acacagaagc gctactgtat
      781 ttgacggaaa aggagcattt tcccatgagg ataagaagat aataattacg gttatgaaca
      841 gagctcaggc tgttcattta aggctgtata taaagaaggt ggatccggaa gctttcatat
      901 taattaccaa tacttcagaa attataggga aaggctttag ggcttgatgg tataatgtac
      961 agaacagatg gaggtgtacg atggtaagga cgatagattt caatttttca ccagacaatg
     1021 atgcaagaac aaatacaaca gctctgcaag aagcagtcga tagaacaggc actataacaa
     1081 tagaagaacc aggcatttat gatatagatg actccattgt cataccttca gatacagcac
     1141 ttgtatttgg taagggcgtg tatataagaa ggacgactga tacaggatat gtattcataa
     1201 acaagggagc ctatacaaga gaatatgacc ataatatttc cataacaggg cttaatcttt
     1261 tatgtaacgg gcataactca aaacaaggca acctcataac cggccttaga gggcaggtat
     1321 gtttctttta tattaaggat ttagtcctac gagacctatt gtgtttagac cttatggaat
     1381 atagcttttg catacaggtg tgtacatttg aaaacctttt aatagagaat gtgcatattg
     1441 aaggcttaaa agatggtata catatagata aaggctgcaa gtttgcaata aggcacggta
     1501 tatttaaaac gtttgatgac cccatagcta taaacgcaca tgattacacg accggttctc
     1561 cagaaatggg atggatagag gacgggataa tagaagactg ttatgacctt gaacaggaaa
     1621 agactgtagg atacttctgc agggtgctgg cagggtcatg gctggactgg tacaaaggca
     1681 tgttagtaca gcagtcagat actgtcgtat acaataacag aatgtacagg gtaaacatgg
     1741 agccaaatga catcttttat acatcaaaca ctcctccaag ccacgaagaa ggaacaaaag
     1801 aatatgacgg tataaaatgg acaatgactc aggataaagg gattgtatat aactgcggat
     1861 gcaaaaatat ccattttcga tatatctatt taaaaaagaa aagacataca gcattctctc
     1921 tgacttttga taaaacaaag tattccagag gctgttatcc taattcaata cctcctgtac
     1981 aggaagatat aacattcgac catatatatg tgcaaaatga gattactgac cttataggta
     2041 tcagaactcc tgttaacact ataaagctta ctaattcagc aataggcaat aacaggatta
     2101 atcttagaaa tgttgaagaa gagggaataa catatatacc agcccatata ctcatatcca
     2161 atgtaacttt caaaggagac ggtaaacaaa gccttgttta tgcagcagaa ggcagaaaag
     2221 ccacattgaa gataaacggc tccataaaag agaacagtac ctatgaacct gttatagagg
     2281 gcgatattga tgttctggag aaggatatat agctgatatt atccgccaac ttgcacatca
     2341 aaactgcgcc aacttgcaca ttgaaaacgc gccaacttgc acatttgtgt tgctatgttt
     2401 tatataaata catgaatttt acaattcttc atattggata ttttgttttt ctgtatacct
     2461 gacaaaagct caatccgtgt ttttcgacag tttcattcgg tgtttttcga cggatacaat
     2521 ccatgttttt cggagtaagt agtgcttttg ccttctcctt gtctgataat aaggcctttt
     2581 tctaataaat tagctaatac acgtactgtt gttgcctgag ataatcctga gtaattctca
     2641 atttcctttc tggtggaata atatttatct ttaagataat caagcacttt ctgttcatta
     2701 taagaaattg cgttttcttt aattataaat tcatgtttgt aattaatatt tggcagagtg
     2761 attttaaatg cattatcaga tacttctatt ttcggtttta tataaaaatc atcataagat
     2821 tccattattt tctgaatacc tgttccatat gcttctatta aatgaagacg atagaatata
     2881 tcgcaaattc tactgtttct taaaaccgat atgccaagca tcatatcatt gtaggtaata
     2941 ccttttggta atcctccaag tgatacaaat tcaattctgt catcaaacaa acttattaac
     3001 gtactgctgc tgtaagaata atctctgtgt gtaattgcat tcagtaaggc ttctctgact
     3061 gcttcaacag ggtaatcagg gatatcaatc ctgtccaatc cggaaaattc tgacctgatt
     3121 ctgttaaaga gttcaatata tttaaataca tcatctatct gtctaaggat agatccggaa
     3181 aattcctttc ggtctttgaa agtcagtttg tttatgcctt gaaatatagc tagcttaata
     3241 gtatgaatgc attggtcaga aaatatcaga ccaagatttg tatacaatcc atctgaattt
     3301 ttaataccca gtgatctttt ttgttcttcc ttgaattcaa cttttctttc cgtgaaatac
     3361 ctcatagctt cattaaaaga caaatcctga ataatggatt tagttgcttc gaattcatat
     3421 atataatcgc ttttatactc tgttgtcata ttcttaatac ctatggatat catctctttc
     3481 taatcagatt atacatttgc taatcataaa agcaaagaaa aatgattaga aatgattaga
     3541 agtgattaga attcaataga tatgaagctt gcaggagtaa gttataagcg ttgctttata
     3601 tcctgagcga tgctagggtc attcaatttt gttacggcaa agcatttttt acctgcatcc
     3661 ttcaatgaag caccaatatg atatacatca atattatcaa taatcatgaa acggtcatga
     3721 aaatgtgtgt tatagcaaac tgtaagtctt gggtattgtt tgttaaaatt attaatatcg
     3781 ttttttgaaa gtttactttt tggtagagta tatatatcta ctgttacacc tgttttcttt
     3841 tttgtaaaaa tgtttagtgt atcaatatct acatatcaat caataagtat tatgctggtt
     3901 gtagcttttg atactaaatt aataaaaaag ctaaaagcgt cgaagatttg actttcgtaa
     3961 aatattttct gttcagattc ggtatgtgct gctatataat caaagacctg tttgaatttt
     4021 ttatcagatt catttatata tttctcctgc ttcatttcaa gctcagttat ataggtgaaa
     4081 agcattgtat tgttagcgat gaatcgacgc atggtaacga atgtgcgcat aatttctaca
     4141 ttgacttttg ctgcaatttc gctacgcaaa actgatgcaa gcatagctat accctgttca
     4201 gtgaagacat agggggtata ccttctacca ccatgactag tgtttccact gtcttctttc
     4261 tttgagatgc caatttggca tctcaaagag gttaattctt caattgtcaa ttggaaacaa
     4321 aaatcatctg gaaaacgata tttattacgt ttgactgctc tatttaaagc tccagtttcc
     4381 acttgataca gcatagctaa atcactgtct agcataacct gctgattgcg aatgacataa
     4441 actaactgtt gtatcttctc tgttgaaagt aaaggtacag ggagagattc actcatataa
     4501 acgcactcct aaataaaaat actagatata aaaatctatg ttaattataa ccttaatatg
     4561 acagattgaa caataatatt tagataaata ttaaaatatg tattaataga atgctttagt
     4621 tcagagtaat ataaaattca ataaaagctt tatctatcgt ttatgaattt tacgaatata
     4681 aataaatatt tggattaacc gatattgttt catcataaga tgcaaataga agagtaatta
     4741 tattttctta ttctcgtatt gatcaataag ttttcaggct ttatctgata taattaaata
     4801 caatgatgaa caagataata actgatatta cagaatacat atttgtagaa gataaaccct
     4861 taaaggctga tatatataac aaagaatatt tgactgactg cgaattctat gtggatgttt
     4921 taataa
//
LOCUS       DAOSXM010000122         4757 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2111
            NODE_12120_length_4757_cov_10.475330, whole genome shotgun
            sequence.
ACCESSION   DAOSXM010000122 DAOSXM010000000
VERSION     DAOSXM010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864178
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4757)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4757)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:24:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,261
            CDSs (total)                      :: 2,229
            Genes (coding)                    :: 2,218
            CDSs (with protein)               :: 2,218
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 3 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4757
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12120_length_4757_cov_10.475330"
                     /isolate="AMR_MDS_2111"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1589)
                     /locus_tag="PLQ68_10525"
     CDS             complement(<1..1589)
                     /locus_tag="PLQ68_10525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009615304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HXK73129.1"
                     /translation="MNIIRVSSILLNLESSLDEVKSAAAKRLNIKISDIVDIKIIKKA
                     VYDKNIDDIKFKYTVDVILSDCIKYTLRHVDKDISYTKLVNYVPITSNIEKKTENPLI
                     VGSGPAGLFCALILAEAGLCPVIIERGKDTDNRDLHIKELIENGILNEQTNIQFGEGG
                     AGTYSDGKLKIGMMTPIKHKILDEFVKAGADPEIKYLEKPHIGTDKLKQILKGLRKYI
                     ESLGGTYKFETKMTDIIIKDNKISGIRTELLNNQTVINSNTVILATGHSARDTFEMLY
                     HLNIPMNCRPFGIGLRIEHHQEYINRIRYKKFSKHSELIPADYHLVTHLKNTRSVYSF
                     CMCPGGYVIPATSENNCLVTNGMSLQKRDGINANSALLVSITEKDFKSQHPLAGIEFQ
                     RNLEKKAFSVGGGNYTAPIQRLEDFMNNKLSKRLGDVLPTYKPDTRFVKSNEYLPEYV
                     SDSIRFAVKDMDNWLNGFYYPDAVLTGIETRSTSPIKIERDKYMQCPVVNGLFFCGEG
                     SGYSGGIISSAADGINCANNV"
     gene            complement(1699..3045)
                     /locus_tag="PLQ68_10530"
     CDS             complement(1699..3045)
                     /locus_tag="PLQ68_10530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018143348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HXK73130.1"
                     /translation="MFKLKENGTKVSTEILAGLTTFVTMAYIIAVNPGILSQAGMEWG
                     AVFLATIIAAIIGTLIMGLVANVPYAQAPGMGLNALFVFTVVFTYGFSWQHALSMVFI
                     CGLINILITVTKIRKHIIKAIPKSLQNAIGGGIGIFIAYVGLLNVGVINFGAGVPGMS
                     IINTGGLYVFFIGLVITIVLLLLKVKGAILIGIIVSTLIGIPLGVTTSASSVSFSDAV
                     NVLPTTFGVIFTKAGLGSLFSDLSRLPIVLLTIFAFSLSDTFDTIGTFIGTGRQTGIF
                     SEEDEKAMESSSGFKSKMDKALFADATATSIGAIFGTSNTTTYVESASGIAVGGRTGL
                     VSVVVAICFAVSAFFATFISAIPSAATAPALVIVGIMMLSAFKEIEWTDLAEAIPAFF
                     TGVFMALAYSITSGIAMGFIFYIIVKICKKQVKEISPVLWVASALFVLNFVLQAII"
     gene            3353..>3553
                     /locus_tag="PLQ68_10535"
     CDS             3353..>3553
                     /locus_tag="PLQ68_10535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003445077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HXK73131.1"
                     /translation="MSKTAIVTGGSRGLGKAMVKKLASMGYNVVVNFVSDRSREGTLE
                     LIDEIEKEYKIKGIAIQGDVSDY"
     assembly_gap    3554..3653
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3829..>4757)
                     /locus_tag="PLQ68_10540"
     CDS             complement(3829..>4757)
                     /locus_tag="PLQ68_10540"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HXK73132.1"
                     /translation="AICDLVEEKAKRAADKYSIPKTYVLMNEMLEKEEIDGVFCLVQP
                     DLLYRVAYDCLSAGKDIFMEKPAGLNSYQAESLARRASSLDKLIQVGMNRRYVPLVQY
                     VFDKMKKTTVINQVDGCFIKHTDIAEIWHFTDAFSCDIVHAADLVRYFAGAEVTDAAS
                     VIARNNSPVDNSWSCVMRFENGVTGTLRGNYQTAARIHNFEIHGADASAYINLGFPGT
                     KGCDATIIYASGKNIYSQAAAGASVSNIEHIDGIELSKGNDSLYSHYGYKQEDEAFIR
                     YLMNKEKPICLIDDAAKSMKLVEMLVENRINA"
BASE COUNT         1444 a          852 c          744 g         1617 t
ORIGIN      
        1 attacattat ttgcacagtt tataccatcg gctgcggatg aaataatgcc acctgagtat
       61 ccagaacctt ctccacagaa gaataaccca tttacgacag gacattgcat atacttatct
      121 cgttctattt ttattggtga tgtggatctg gtttcaatac ctgttaaaac tgcatctggg
      181 taataaaatc catttaacca gttatccata tccttaactg caaaccgtat tgaatcagac
      241 acatattctg gtagatattc attgctttta acaaatctgg tatcaggttt gtaagttggc
      301 aaaacatcac caagcctttt gcttagtttg ttattcatga aatcctcaag cctttgtatt
      361 ggagcagtat aatttcctcc tccaacagaa aatgctttct tttctaagtt tctttgaaat
      421 tctattcctg caagcggatg ctgtgatttg aaatcttttt cagttattga tactaacaat
      481 gcgctattag catttattcc atctcttttt tgtaaactca ttccattggt aacaagacag
      541 ttattttctg atgtagcagg tattacataa ccacctgggc acatgcagaa tgaataaacg
      601 ctacgggtat tcttcaaatg agttaccaaa tgataatcag ctggaatcag ttcggaatgt
      661 ttactgaatt ttttatatct aattctattt atatactctt gatgatgttc aattctcaaa
      721 cctataccaa aaggtctgca attcattggt atattcaaat gatacaacat ttcaaaggta
      781 tctcttgcac tatgtccggt agctagtatt acagtatttg aattaattac tgtctgattg
      841 tttaataact cagttcttat tcctgatatt ttattatctt ttataataat atcagtcatt
      901 tttgtttcaa atttatatgt tccacctaat gattctatgt attttcttaa tcccttaagt
      961 atttgtttta atttatctgt accgatgtgt ggtttttcta aatatttaat ttctggatct
     1021 gcacctgctt ttacaaattc atcaagtatc ttatgcttta tcggtgtcat cattccaatt
     1081 ttcagttttc catctgagta ggttccggct ccaccttcgc caaactggat gttggtttgc
     1141 tcattcaata tcccattttc tattaattct ttgatgtgca aatctctgtt atctgtatct
     1201 tttcctcttt ctataattac agggcataat cctgcttctg caagtataag agcacagaat
     1261 agtcctgctg gaccggagcc aactattaaa ggattttctg ttttcttttc aatgtttgaa
     1321 gttattggaa cataattaac cagtttagta tatgaaatat ctttatcaac atgtctaaga
     1381 gtatatttaa tgcaatctga taaaattaca tcaacagtat atttgaattt aatatcatct
     1441 atattcttat cataaactgc tttctttatt atttttatat caactatatc actaatcttt
     1501 atattcagtc tttttgctgc agctgatttt acttcatcaa gtgaactttc cagatttaac
     1561 aatattgatg atacccgaat tatattcata tattacacaa tccttgtttt aatatcatag
     1621 tatttagttt agcatagatt taagaaaagg tataaaaaaa agctgcccgc taaaggcagc
     1681 aattttttat tatctcttct aaataattgc ttgaagtaca aagttaagta caaacagagc
     1741 agaagctacc cacagaaccg gggatatctc tttaacctgt tttttgcata tcttaacaat
     1801 gatgtagaat atgaagccca ttgcgatacc tgaagtaata gagtatgcca aggccatgaa
     1861 cacgcctgta aagaaagcag gaattgcttc tgcaaggtct gtccattcaa tctctttgaa
     1921 tgctgatagc atcattatac caacaataac aagagcaggt gctgttgctg cagaaggtat
     1981 ggcacttata aatgttgcaa agaatgcgct tactgcaaaa caaattgcaa caacgacact
     2041 tacaagacct gtacgtccgc caacagcaat accagaagca ctctcaacat atgttgttgt
     2101 atttgatgtt ccaaaaatag cacctattga tgtagctgtt gcatctgcaa ataaagcttt
     2161 gtccattttg gatttgaatc ctgaactaga ttccattgct ttttcatctt cttcactgaa
     2221 aattccagtc tgacggcctg ttcctataaa tgttccgatt gtatcaaatg tatcagataa
     2281 actgaatgcg aaaatagtca gtagaactat aggaagtctt gaaagatcag aaaacagtga
     2341 acctaaaccg gcttttgtaa agattacgcc gaatgttgtt ggaaggacat ttactgcatc
     2401 ggaaaaactt acagatgaag cagatgttgt aactcctaat ggaataccta tgagtgttga
     2461 tactattata cctataagta tagctccttt aacttttagt aatagtaaaa ctattgtgat
     2521 taccaaaccg ataaagaaaa catataatcc acctgtgtta ataattgaca ttcctggaac
     2581 tcctgcgcca aaattaataa cacctacatt aagcaatcca acataagcta tgaatatacc
     2641 gataccacca ccaatagcat tctgaaggct ttttggtata gctttaatga tatgcttacg
     2701 aatttttgtt actgtgatga gtatgttaat aagtccgcaa atgaaaacca tggacagagc
     2761 atgttgccat gagaagccat aagtaaatac tacagtaaac acgaataaag catttaatcc
     2821 cattcctggg gcctgtgcat atggtacatt agcgacaaga cccattatca aagtaccgat
     2881 tattgctgca attatggttg caaggaaaac agcaccccat tccatacctg cttgtgacaa
     2941 tattcccggg ttaacagcga taatgtaagc cattgtaacg aatgttgtta ggcccgctaa
     3001 aatttccgtt gacacttttg tgccattctc ttttagcttg aacattttaa atcccccttt
     3061 tgaatttaat ataatgtaag tatattacaa ggtaataaat tgtcaatatt aaaccgttaa
     3121 aaaatatatc atttttttgt tttcccacaa catgttgtat aaatactgac aaaaaacagt
     3181 atatattgag gctgctactt ttatataact gtaatttaat aaaaaaataa ccgaaagttc
     3241 gaaatttgtt caagatttat gttgcaattg caacagactg tttttgaatc ataccttata
     3301 cttcatttag cattaaaagt gcttatcaaa taaaagaaag gctggtataa atatgagcaa
     3361 gacagcaata gtgacaggtg gttcaagagg tttaggaaaa gctatggtta aaaaacttgc
     3421 ttcaatggga tataacgttg tggttaattt tgtaagtgac aggagcaggg aaggaacttt
     3481 agaacttatt gatgaaattg agaaagagta taagattaaa ggcatagcaa ttcaggggga
     3541 tgtctctgat tatnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnattgatg
     3661 ctaaatatgt tacaggacaa acgatctcac ctaacggcgg tatattaatg ccataagatg
     3721 atatctttac atattgtcat ttgcgttgtt acgcataata aagggagcat atcagctccc
     3781 tttatgttta tacatctata tggttattaa tagtatcttt gtttattctt atgcattaat
     3841 cctgttttca acaagcattt caaccagttt catggatttt gcagcatcat caatcaggca
     3901 gattggtttt tccttgttca tcagatatct tatgaatgcc tcgtcctctt gtttatagcc
     3961 ataatgtgaa tataaagaat cattaccttt actaagctca attccatcta tgtgctctat
     4021 attggatact gatgcacctg ctgcagcttg tgaataaata tttttacctg atgcgtatat
     4081 tatcgtcgca tcacatcctt ttgttcctgg gaatccaaga tttatgtatg cgcttgcatc
     4141 tgctccatga atttcgaaat tgtgaattct agcagctgtc tgataatttc ctcttaatgt
     4201 accggttact ccgttttcaa atctcataac acagctccag ctgttatcaa caggactgtt
     4261 gtttcttgcg ataacgcttg ctgcatcggt gacttcggca cctgcaaaat atcgcactaa
     4321 atcagcagca tgtacaatat cgcaggaaaa tgcatcagtg aaatgccaaa tttcagctat
     4381 atcagtatgt ttaatgaaac aaccgtcaac ctgatttatt actgtagttt ttttcatttt
     4441 gtcaaataca tactgtacta atggtacata acgtctgttc attccaactt gtataagttt
     4501 atctagtgaa gatgctcttc ttgctagtga ctcagcttgg tatgaattca atcctgctgg
     4561 tttttccatg aatatgtctt tacctgcaga taaacagtca taagcgaccc tgtagagaag
     4621 gtctggttgt acaagacaga acactccgtc aatttcttcc ttttccagca tttcattcat
     4681 aagtacataa gtctttggta ttgaatactt atcagctgct ctttttgctt tctcttcaac
     4741 aagatcacat attgcga
//
LOCUS       DAOSXM010000123         4651 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2111
            NODE_12528_length_4651_cov_11.175805, whole genome shotgun
            sequence.
ACCESSION   DAOSXM010000123 DAOSXM010000000
VERSION     DAOSXM010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864178
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4651)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4651)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:24:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,261
            CDSs (total)                      :: 2,229
            Genes (coding)                    :: 2,218
            CDSs (with protein)               :: 2,218
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 3 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4651
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12528_length_4651_cov_11.175805"
                     /isolate="AMR_MDS_2111"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(63..905)
                     /locus_tag="PLQ68_10545"
     CDS             complement(63..905)
                     /locus_tag="PLQ68_10545"
                     /inference="COORDINATES: protein motif:HMM:NF024702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HXK73133.1"
                     /translation="MNRRIRIVGMLLVLVLIFGILTSCSTTKENLFSINIVNSKEIQI
                     TKYHGKSKTVVIPDMINDLPVTGITPNAFWGASEIKSIVLPETVVTIGNNAFFSCVSL
                     TQFNIPKNLKSLGENVFSGCENLKEITVSDGNEYFSSIDGVLFDKDQQKLIIYPAKKT
                     GDFYQIPLTVEYIGKNAFSNCEMLQQVVYPDSLIEIGQNAFKNCINLAEIEIPEGTLN
                     LRAGAFWECVNLSSIKIPSSVDNIGEHIFYGCAMLSSIYVKEGSYAQQWCEDNSRTAA
                     MKFY"
     gene            1060..2484
                     /locus_tag="PLQ68_10550"
     CDS             1060..2484
                     /locus_tag="PLQ68_10550"
                     /inference="COORDINATES: protein motif:HMM:NF012885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-isopropylmalate synthase regulatory
                     domain-containing protein"
                     /protein_id="HXK73134.1"
                     /translation="MKTVVLIKDCTLLNSETDKSFKISFKEKLEIAKQLENINTDIIE
                     TGYADGSQAELIAVKTIAGSLRKSILCCPSKLNKTSIDKTYEAVKCAVKPRIHISAPV
                     SAVNMEYQLHMKKDQLFENIKECVNYAKSLCDDIEFTALDATRADKTLLIEVLKIAIS
                     SGASTVGIEDSVGHMMPEEVFLFIDDLKKQLSIDNSICLSFKAKNDLGMAVANSVCAI
                     KSGANQISCSINGLANDASLEDTATAILTRSDDISITSNITPSQINRASVLLSGISDT
                     KNIKSKGISLNNKVNEETELLDKQMDTYAFRTIISDLGYSLTDKDIERIYDMFKELAE
                     KKQQIYIKDIEAIIGREAIQVPRVYQLDRFVINSGNKITSTAAVKLKYNEKTLEGVAF
                     GDGPIDAAFHVIGKIMGMDIELFDFNLRSVTEGKDALGAATVKIRYNGNIYIGRGLST
                     DVIESSIMAYINAVNKMLYEEGIQ"
     gene            2481..>4651
                     /locus_tag="PLQ68_10555"
     CDS             2481..>4651
                     /locus_tag="PLQ68_10555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003520602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HXK73135.1"
                     /translation="MKYAFSTISWTSFTWKEAVAMAKDLGFSGIEVRGLENNVYAVDA
                     RPFSSQQVLKTAEQLANLHLEVSCFSTACNLNDIYKAKENVHQLKAYIDLAAKMGTSY
                     VRLLSDDSIEPNETATDDAVISVITEILPYAEKKHVTLLLETIGIYCDTDRLRKVLEH
                     FASDNLAALWDIHHPYRFNNESPEKTVQNLGAYIKYVHMKDSVINDNQLEYRLMGEGD
                     LPIEEIVGALKSINYDGYISYEWIMSWSSDLDDPGIVFPHFMNYITSKYEDNKNTKKL
                     YYNKTKTGRFIWKKEELIDFTFPQVLDKMVEEFPDQYAFRYTTLDYTRTYSQFRDDVD
                     KFARSLITLGVKRGDHVAVWATNIPQWFITFWATTKIGAVLVTVNTAYKIHEAEYLFR
                     QSDTHTIVMIEGYKDSPYAEIMKELCPELKNSVPGKPLHIKRLPFLRNIITVDFSMDG
                     CISWEDAMDMHKKTPIEEVYRRASTINKDDVCNMQYTSGTTGFPKGVMLTHYNIVNNG
                     KCIGDCMDLSTADRMLIHVPMFHCFGMVLAMTASVTHGTTMSPLPYFSPRPALNCINL
                     EKITCCHGVPTMFIAMLEHEDFAKTDFSYMRTGIMAGSPCPIKVMQDVQEKMNMKEIT
                     IVYGQTEASPGCTQSTTDDPIEIRVNTVGKALPGVECKIVDPETNKELPDDTDGEFVA
                     RGYNIMKGYYKMPMATSAAIDKDGWLHTGDLARKHNGYYKIT"
BASE COUNT         1682 a          789 c          825 g         1355 t
ORIGIN      
        1 ctcatactta taaaagatac tatcatatga atatactttt ttcaacagtt tatatatcct
       61 tactaataaa acttcattgc tgcagttctg gagttatcct cgcaccattg ctgagcatat
      121 gacccttcct tgacataaat ggatgaaagc attgcacatc cataaaaaat atgctcccct
      181 atattatcaa cactggaagg tattttgatg cttgaaagat tgacacattc ccaaaatgct
      241 ccagctctta agtttaatgt accttcagga atctcaatct ctgctaaatt tatacagttt
      301 ttaaacgcat tctgtccaat ttcaattaaa ctatcaggat atacaacctg ctgaagcatt
      361 tcacaattag agaaggcatt tttacctata tattctactg ttaaaggaat ctgataaaaa
      421 tcaccggttt tttttgcagg atatataata agcttctgct ggtctttatc aaaaagaact
      481 ccatctatag atgaaaaata ctcatttcca tcactgactg ttatttcttt aagattttca
      541 catcccgaaa aaacattttc tcctaaactc ttcagatttt ttggaatatt aaactgtgtt
      601 aatgatacgc agctgaaaaa ggcattatta ccaatagtaa caaccgtttc tggtaatact
      661 attgatttta tttcagaagc cccccaaaaa gcattagggg ttataccagt gacaggtaaa
      721 tcatttatca tatcaggaat tacaactgtt ttactttttc catgatattt ggttatttgt
      781 atttccttac tgttaacaat attaatagaa aaaaggtttt cttttgttgt actgcacgat
      841 gtaagtattc caaaaatcaa taccagtact aaaagcattc caactatcct tattcgtctg
      901 ttcatattac ctccgttcat ttttagtgct taatatatat accacaaatg tgacagattt
      961 gtcatcaaaa cataaaaaac tcagaaaaaa tagacttttt tcttatgtaa tgctataatc
     1021 ccaaattaat aacaagcaga agtatggaag gggttttata tgaaaaccgt ggttttaatc
     1081 aaagattgca cacttttaaa cagtgaaaca gataaatcat ttaaaattag ttttaaagaa
     1141 aagttggaaa tcgcaaaaca actggagaac ataaatacag atataattga gactggttat
     1201 gctgatggct ctcaagcaga acttatagct gttaaaacaa tagcaggctc acttagaaaa
     1261 agcatattat gctgtccttc taaactgaat aagacaagca tagataagac atatgaagct
     1321 gttaaatgtg cagtaaaacc aagaattcac atatctgctc ccgtatcagc agtaaatatg
     1381 gaatatcagt tacatatgaa gaaagaccag ttatttgaaa atataaagga atgtgtaaac
     1441 tatgcaaaaa gcttatgtga cgatattgag ttcacagctc ttgatgcaac aagagctgac
     1501 aaaactttac taattgaagt actgaaaatc gctataagtt caggagcatc tacagtagga
     1561 atagaagatt cagtcggaca catgatgcca gaagaagtct ttttgtttat tgatgatctt
     1621 aaaaaacagt tatcaattga taatagcata tgtctttctt ttaaagcaaa gaatgattta
     1681 ggtatggcag tagccaattc tgtttgtgca ataaaatcag gcgctaatca gatatcctgt
     1741 tccattaacg gattagccaa tgatgccagc ctggaagata ccgcaacagc aattttaaca
     1801 agaagcgatg atatatccat taccagtaac ataacaccat cacagattaa cagggcttct
     1861 gtgctcttat ctggaatatc agatactaaa aatattaaat caaaaggcat atcccttaat
     1921 aacaaagtaa atgaagaaac agagctttta gataagcaga tggacacata tgctttcaga
     1981 actattattt ctgatttagg atacagcttg acagacaaag atattgaaag aatatatgat
     2041 atgttcaagg aacttgctga aaaaaagcag cagatttaca taaaagacat agaagctatt
     2101 ataggaagag aagcaataca agtcccaaga gtatatcagc tagacagatt tgtcataaac
     2161 agcggaaaca agataacctc cactgctgca gtaaaactga aatacaatga aaaaactctt
     2221 gagggtgtgg ctttcggtga cggacctatt gatgctgcct ttcatgtaat tggaaagata
     2281 atgggtatgg atatagagct atttgatttt aatttaaggt cagttaccga aggtaaagat
     2341 gccttaggtg ccgcaacagt caaaatcaga tataacggta atatatatat aggaagagga
     2401 ttgtctactg atgtaataga atcttccatc atggcataca tcaatgctgt aaataagatg
     2461 ctctatgagg aaggtattca atgaaatacg cgttttcaac aataagctgg accagcttta
     2521 catggaaaga agctgttgct atggcaaaag acttaggttt ttctggaatt gaagttcgag
     2581 gactggaaaa taacgtatat gcagtagatg caagaccttt ttcttcgcaa caggtattaa
     2641 aaaccgcaga acagcttgct aacctgcatt tggaagtatc atgtttttca actgcttgta
     2701 atttaaatga catttataaa gcaaaggaaa atgttcatca gttaaaagca tatattgatc
     2761 ttgcagctaa aatgggaaca tcatatgtaa ggcttttatc tgatgattca atagaaccaa
     2821 atgaaacagc aactgatgat gctgtaattt cagtaataac agaaatactt ccctatgctg
     2881 aaaagaagca tgtaacctta cttttagaaa caatcggcat ttactgtgat acagacagat
     2941 taagaaaagt tcttgaacat ttcgcatcag ataatcttgc agcattatgg gatatccatc
     3001 atccttacag atttaataat gagtcaccgg aaaaaacagt acagaattta ggagcatata
     3061 taaaatatgt gcacatgaaa gattctgtaa ttaatgataa tcaactggaa tacagactca
     3121 tgggtgaagg tgaccttcct atagaagaaa tagtaggagc attaaaatct ataaattacg
     3181 atggatatat atcatatgag tggataatga gctggtcatc tgatttagat gaccctggaa
     3241 tagtatttcc tcatttcatg aattacatta catcaaaata cgaggataac aaaaacacaa
     3301 aaaaacttta ctacaataaa acaaaaacag gccgcttcat ctggaaaaag gaagaactta
     3361 ttgatttcac atttccacag gtactggaca aaatggtaga agagttccct gaccagtatg
     3421 ctttccgtta tacaactctt gactatacca ggacatattc acagtttcgt gatgatgtag
     3481 ataaattcgc ccgctcttta ataacacttg gagttaaaag aggagatcat gtagcagtat
     3541 gggccacaaa tatacctcag tggtttataa cattctgggc aacaactaaa attggtgcag
     3601 ttctggtaac cgttaatact gcatacaaga tacatgaagc ggaatacctt ttccgacaat
     3661 ctgatacaca cactatagtg atgatagaag gatacaagga ttcgccatat gcagaaataa
     3721 tgaaagagct atgccctgaa ttaaaaaact cagtaccagg taaaccttta catattaaaa
     3781 gacttccctt tttaaggaat attattacag tagacttttc aatggatgga tgcatatcct
     3841 gggaagatgc aatggatatg cataaaaaga caccaattga agaagtatac agaagagcaa
     3901 gcacaataaa taaagatgat gtctgcaata tgcaatatac atcaggaact acaggctttc
     3961 ctaaaggtgt aatgcttact cattacaata tagtaaacaa tggaaagtgt attggagact
     4021 gtatggattt gtctacagca gacagaatgc ttatccatgt acctatgttc cactgctttg
     4081 gtatggtttt agctatgact gcaagtgtaa cgcatggaac aactatgtcc ccgcttccct
     4141 atttctcccc taggcctgct ctaaactgta ttaatcttga aaagataaca tgctgccatg
     4201 gtgttcctac tatgtttatt gctatgctgg agcatgagga ttttgccaaa acagatttct
     4261 catatatgag aacaggcatt atggccggaa gcccctgccc tataaaagtt atgcaggatg
     4321 tccaggaaaa gatgaacatg aaagaaataa ccatagtata tggacagact gaagcatctc
     4381 ctgggtgcac tcagagtacg actgatgatc ctatagaaat aagggtaaat acagtaggaa
     4441 aggctttgcc aggtgttgaa tgtaaaatcg tcgaccctga aacaaacaag gaacttcctg
     4501 atgatacaga tggagagttt gtagcaagag gatataacat tatgaagggc tactacaaaa
     4561 tgcctatggc gacatctgct gcaattgata aagacggatg gcttcataca ggagatctcg
     4621 ctagaaaaca taacggctat tataaaataa c
//
LOCUS       DAOSXM010000124         4605 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2111
            NODE_12693_length_4605_cov_9.987253, whole genome shotgun sequence.
ACCESSION   DAOSXM010000124 DAOSXM010000000
VERSION     DAOSXM010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864178
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4605)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4605)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:24:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,261
            CDSs (total)                      :: 2,229
            Genes (coding)                    :: 2,218
            CDSs (with protein)               :: 2,218
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 3 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4605
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12693_length_4605_cov_9.987253"
                     /isolate="AMR_MDS_2111"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1204)
                     /locus_tag="PLQ68_10560"
     CDS             complement(<1..1204)
                     /locus_tag="PLQ68_10560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalase family glycosidase"
                     /protein_id="HXK73136.1"
                     /translation="MQIQIPSKEIIEEKKQLVLDRIYKYHLKKMAGSKGNIFLISNTY
                     PGVWLEHVYDAVSWVNYDKSDPSISVNQIKLFLNRQKPDGQFPCYVIDPDKKTGFKNL
                     VGYSQIQECVSFARLCLETCIQNNDTDLLKECYEKCVKWDKWLTENRMTLKTGLIEMF
                     CGFDTGHDNSGRFETIKHKNNFSDDAKDYPHDDNLLPFITPDMNAVFYGSRNALSVMA
                     GKLGLENEAHEWKRAAHDVKKRLIETCYDEFDEFFYDVDSHGNKIKCKSISITNLFCE
                     SLLDMDMAKRIFKRYLNNPNEFDTPYPYPAVSISDPSWKQNLPGNSWGFYSQGLTSLR
                     SLRWMDKYGFSDQLEKNMKAWINAWCSTDKINFGQELHPITAEPSTCGNWYSSTMLFF
                     IHSLKRLGL"
     gene            complement(1302..3674)
                     /locus_tag="PLQ68_10565"
     CDS             complement(1302..3674)
                     /locus_tag="PLQ68_10565"
                     /inference="COORDINATES: protein motif:HMM:NF013239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HXK73137.1"
                     /translation="MKITKNIFPQFIEDAKIAETVKTDRSDSDKSLDYMYTSDMVEFI
                     KASYIKSFKQSKNGIEYVICADKHLNRTVMTHETVMNQMIKKKNEEVVEIKVCINVWS
                     EDIVRIRYGTGSLERKETLPKEQRMLTGKPKRNIKFTVTDYENLITLSTKKIVINIDK
                     SSSVISVYDSNGEYITKQARYAIMPTDAVGMAICFDKNDTACFEGYELTSKEQIYGLG
                     ERFDHVNRRGIKTDFWNKDAFGTSNTRTYINVPFVVSTSGYGIFLNSTAKTQWDIATK
                     EASTLSFCTLEPVMDYFLITGKNIKQIIYNYCTLTGFSPIPPIWSFGLWMSRNSYLSW
                     DVVFDVAKNIRLKEIPCDVLHLDTAWFKDDWNCDLRFSKERFPQPKENIKKLNNLGFK
                     ISAWQYNFIPPREDNENYVEAREKGYLALDKNGNIFKHSNDLPGSWKDDSIIDFSNQD
                     ACQWYASMIKNVIRTGISAIKTDFGEGIPEDAVYKNIEGTKFHNNYSLAYNSVVFNAC
                     KEITNENLVWARSGTAGSQRYPIHWGGDSQCTFEGLQGTIRAGLSLGLSGFVFFSHDI
                     GGFIGRPTPELYIRWAQVGLFSSHSRCHGGGNNNSREPWSFGEEANRIFKKYANLRYS
                     LLPYIVSEANSCAKNAVPMMKAMVLEYESDLNTYNIDDQYMFGNSLLVAPILESLEKK
                     KIRNIYLPKGIWYDYWTKKKIISKGEWIQVSVTLDIMPIFVKNGTILSYKEYGKQNTE
                     ESIYPIKRIESYGKNASYKVTDGKEQLIIKFKNGKLISSISNEVEFLSIF"
     gene            3857..>4605
                     /locus_tag="PLQ68_10570"
     CDS             3857..>4605
                     /locus_tag="PLQ68_10570"
                     /inference="COORDINATES: protein motif:HMM:NF014376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HXK73138.1"
                     /translation="MDVIYTCEVDIEDALFKLSFDEGFYSSPSKPVLLHNHNAYEVHI
                     IEHGEFIFDINSMQFTVSDGDCCVIKPNTYHSKGNTKSDNARKYCFKFEYFTNTNNSF
                     DKSRKTNKFDFLRMSEDVFVSGNCSKSINILKDIKSEMDDLYIGYQTNLKNLSSLLLI
                     DILRGMCCKKISDNNYKHSNLEDSRHAVIDSFFAINYMNDVYATDLADLLSISVRQLS
                     RIMYKYYGLTFKDKLAQIRIFAAKDLLKDKK"
BASE COUNT         1427 a          810 c          695 g         1673 t
ORIGIN      
        1 atagtcctaa tctttttaaa gaatgaataa agaatagcat cgtacttgaa taccagttgc
       61 cgcaagttga aggttctgct gttattggat gcaattcttg accgaaatta atcttatcag
      121 tcgaacacca tgcgtttatc caagccttca tgttcttttc aagctgatcg gaaaaaccat
      181 atttgtccat ccatctcaat gaccttagag aagtaagccc ttgggagtaa aatccccatg
      241 aattacctgg caaattctgc ttccatgaag ggtctgaaat tgagacagca gggtaggggt
      301 acggagtatc gaattcgttc ggattattaa gatatctctt aaaaattctt ttggccatat
      361 ccatatccag aagactttca caaaataaat ttgttattga tattgatttg cattttattt
      421 tattcccatg gctgtctaca tcataaaaga attcgtcaaa ttcatcataa catgtttcta
      481 ttaaacgctt tttaacatca tgagcagctc ttttccattc atgtgcttcg ttttcaagtc
      541 ccagttttcc tgccattact gaaagagcat tccttgaacc gtaaaatact gcattcatgt
      601 caggtgttat aaaaggaaga agattatcat catgtggata atcctttgca tcatcagaaa
      661 aattattttt gtgttttatt gtttcaaatc ttccgctgtt gtcatgtcca gtatcaaatc
      721 cacaaaacat ttcaatgagg ccggttttca aagtcatgcg attttctgta agccatttat
      781 cccacttaac gcatttttca taacactctt ttaacaggtc tgtatcatta ttttgaatac
      841 atgtttcaag acaaagtctt gcaaaggata cacactcttg aatctgacta tagccgacta
      901 aatttttaaa tcctgttttt ttatcaggat caattacata acaggggaac tggccatcag
      961 gtttttgcct attcagaaag agttttattt gattaaccga tatggaagga tcggatttat
     1021 catagtttac ccacgataca gcatcataaa catgttcaag ccagacacct ggatatgtat
     1081 tagatataag aaatatgtta cctttagacc cggccatctt cttcagatgg tatttataaa
     1141 tcctgtctag aacaagttgt tttttttctt caattatttc ttttgacggt atctgtatct
     1201 gcatagtata aattaactcc tttttttgat tacatacctt tcattccaca aattatatcg
     1261 gattatctca tttagtattt cttttctcgt agaattgaat actaaaagat gcttaaaaat
     1321 tctacttcat tactgattga tgatattagt tttccattct taaacttaat tattaactgt
     1381 tcttttccat cagttacttt atatgaagca tttttgccat acgattctat tcttttaata
     1441 ggataaatac tttcctctgt attttgttta ccatactctt tataagagag gattgtgcca
     1501 ttcttaacga atatcggcat aatatcgagt gtcacactaa cctgtatcca ttcacctttt
     1561 gatataattt tcttttttgt ccagtagtca taccatattc ctttaggcag ataaatattt
     1621 cttattttct ttttttctaa agattctaaa attggcgcta ctagaagcga gttgccgaac
     1681 atgtattgat catcaatatt atatgtattt aggtctgatt catattcaag aaccattgct
     1741 ttcatcatgg gtacagcatt ttttgcacag ctattggctt cacttacaat gtatggaagc
     1801 agggaatacc taagattagc atacttttta aatatgcgat tagcttcctc accaaaagac
     1861 catggttctc tactgttgtt gtttccgcca ccatgacatc ttgagtgact ggagaataat
     1921 cctacttgtg cccaacgtat ataaagttct ggcgtcggtc tgccaatgaa gccgcctatg
     1981 tcatgtgaaa agaaaacaaa tccgctcagt cccaaagata atcctgcacg aattgtacct
     2041 tgaagcccct caaatgtaca ttgactgtct ccgccccagt gaataggata tctctggctt
     2101 cctgcagtac ctgaacgagc ccagacaaga ttttcatttg taatttcttt acatgcatta
     2161 aatacaacac tattgtaagc aagtgagtag ttattgtgaa atttagttcc ttctatattt
     2221 ttgtaaacag catcctctgg aattccttct ccgaaatctg ttttaattgc agatatacct
     2281 gttctaatta catttttaat cattgatgcg taccattgac aggcatcttg attggaaaaa
     2341 tcaataattg aatcatcttt ccatgaaccg ggaagatcat tagaatgttt gaaaatatta
     2401 ccatttttat caagagccaa ataacctttt tctcttgctt caacataatt ttcattatcc
     2461 tctcttggcg gtatgaagtt atactgccaa gcggatattt taaatcccag attatttaat
     2521 tttttgatat tttcctttgg ttgtggaaat ctttctttgg aaaaacgcag atcacaattc
     2581 caatcatcct taaaccatgc agtatcaagg tgtagaacgt cgcaaggtat ttcttttaat
     2641 cttatattct ttgcaacatc aaatactaca tcccaggaaa ggtagctatt acggctcatc
     2701 catagaccga aactccaaat tggaggtata ggtgaaaacc ctgtaagagt acagtaatta
     2761 tatattatct gttttatgtt ttttcccgta attaaaaaat agtccattac cggttcaagc
     2821 gtacaaaaag acagtgttga agcttctttt gttgcgatgt cccactgtgt ttttgctgta
     2881 ctatttaaaa atattccata ccctgaagta gacacgacaa atggaacatt gatatatgtc
     2941 ctggtatttg aggtaccaaa tgcatcctta ttccaaaaat ctgttttaat gcctcttctg
     3001 ttaacatgat caaatcgctc tccgagacca tatatctgct cttttgatgt taattcatat
     3061 ccctcaaaac aagctgtatc atttttatcg aaacatattg ccattcctac tgcgtctgtt
     3121 ggcataattg cgtatcgggc ttgtttggtt atatactcac catttgaatc atatacagat
     3181 ataacagatg agcttttatc aatattaatt acaatctttt ttgttgatag tgttatgaga
     3241 ttttcgtaat cggtaactgt aaatttaata tttcttttag gttttcctgt cagcattctt
     3301 tgttctttag gtaatgtttc ttttctttca agtgaaccgg ttccgtacct tatccgcact
     3361 atatcttcag accacacatt aatacaaact tttatctcta caacttcttc attctttttc
     3421 ttaatcatct gattcattac tgtctcatgg gtcataacag ttctgttaag atgcttatct
     3481 gcacagataa catattctat accatttttg ctttgtttaa atgattttat atacgatgct
     3541 ttgataaact caaccatatc cgacgtatac atataatcaa gagatttatc tgaatctgaa
     3601 cggtcggtct taacagtttc tgctattttt gcatcttcaa taaattgcgg aaaaatattt
     3661 tttgttattt tcatggtata cccccccata tggattacag aaagtatatc atattaatca
     3721 ctgaaaaact caaaaacaat tttttaaatt tagtattatt ctcatatatt attgttcaat
     3781 gatactataa acaagttata attaattatg aaagctgatt tattgttatt tcatttctaa
     3841 gatagggatt aatgctatgg atgtcattta tacttgtgaa gtagatattg aggatgcctt
     3901 atttaagctc tcttttgatg agggatttta ttcatcacca agcaaacctg tattacttca
     3961 taatcataat gcatacgaag ttcatattat tgaacatggt gaatttattt ttgatataaa
     4021 tagcatgcag tttactgttt cagacgggga ctgttgtgtt ataaaaccaa atacatatca
     4081 ttcaaaaggt aacactaaat cagataatgc aagaaagtac tgttttaaat ttgaatattt
     4141 tacaaatacg aataattcct ttgacaaatc aagaaaaacg aataaatttg attttttaag
     4201 aatgtcagag gatgtttttg taagcggaaa ttgcagtaag tcaataaata tacttaaaga
     4261 tataaaatct gaaatggatg atttgtatat aggataccag acaaacttga aaaatctttc
     4321 atctctgctt cttatcgata tactaagagg catgtgttgc aagaaaatta gtgacaataa
     4381 ttataaacat agtaatttag aagacagcag acatgctgtt attgatagtt tctttgcaat
     4441 aaactatatg aatgatgttt atgcaaccga tcttgctgat ttgctaagta taagtgtacg
     4501 tcaattgagt cgaataatgt ataaatatta tggattaaca tttaaagaca aacttgctca
     4561 gattagaata tttgcagcaa aggatttatt aaaagacaaa aagat
//
LOCUS       DAOSXM010000125         4470 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2111
            NODE_13226_length_4470_cov_11.335447, whole genome shotgun
            sequence.
ACCESSION   DAOSXM010000125 DAOSXM010000000
VERSION     DAOSXM010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864178
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4470)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4470)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:24:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,261
            CDSs (total)                      :: 2,229
            Genes (coding)                    :: 2,218
            CDSs (with protein)               :: 2,218
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 3 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4470
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13226_length_4470_cov_11.335447"
                     /isolate="AMR_MDS_2111"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2321)
                     /locus_tag="PLQ68_10575"
     CDS             complement(<1..2321)
                     /locus_tag="PLQ68_10575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK73139.1"
                     /translation="MKKKVISVLLVLCMVFALIPMTVQAAVPLTILNIGGTNVTLPSH
                     GNTTFSDTDISDSTWWVTNNSGSYTLTLNNSNISTPHSWDVSYSSYTAAIYADGDLNL
                     VLQGTNTVNNPDALTTGLNKYAIGIYIKGLLNISGSGTLNVSSDIIAAGYCFGLFIMG
                     NLTISENVTLDARASDNSYAYRNFGIYCAGTVTIRNYASVSAIGGDIPTITTDNILSE
                     GLYLYNLYVYDNAKLSAIGGSLNGSNSSSMGIDLAASSATIYISTKAMVYVKSGSAGL
                     RNSYGISSSNDYSSGSLTVNGGNLTAISSSSTWVSAGIHMAGAININGGTVISSGGKG
                     DHSSYGIYSQRNDITISNSTVVATAGSVSTYNSRAICANQTDRNINIDNNSLVIAKAT
                     AVNASALNRDISGVISGSYNGKLAVIGDSSLYKVLAGSLDLTTGDHSTKTLADDGYTW
                     DNGTNTLTLRNIVVNGSADGITTDTFGIKAPADTDILLEGKNVVCAGDTTGSVSHGIN
                     TAGGSMTLSGNGEIIALGGHSQDYSYGLYSDTFTNLNGGSGISLAAQANSINGALNLS
                     PSTTNTDAVRTLTAGGWAERVAVWYVTNPLDTPTAVFSATGTNTGTLSNVTTSMKYSV
                     DGGSNWINITGTTMDITGVNSSNDIKVFVLGNGTTTSDSSIQMIDITQPFSPSVTSST
                     CTTLANNDGKLLGVTSAMEYKPVAAAEWTSGTGSDITGLSNGIYYVRIKAAGTSLASN
                     NHIISITRYTGIQEATPSATFTATGSDTGTLSNV"
     gene            2538..3293
                     /locus_tag="PLQ68_10580"
     CDS             2538..3293
                     /locus_tag="PLQ68_10580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004615913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HXK73140.1"
                     /translation="MLRIAICDDNSRDLSHITSLVESYKKNKKTDIVYMSFHNAIDLL
                     ESLKKKECDLLLLDVLMPGFNGLQAAHELRKQNDKIEIIFLTSSPEYAVESYNVRAHH
                     YLLKPATEDKLFPLLDKLIEKLKKPEEAIIVKSSSSVFTIPYKNIEYVEINAKTIYFY
                     LTDNDVKKVSGSMAEYEALLLKRSGFIKVHRSYLVNLQWVQQLNQKELITLTGRKVPI
                     ARTVYTQIRTAYTQFLFKEADELTNTKGGINRD"
     gene            3721..>4470
                     /locus_tag="PLQ68_10585"
     CDS             3721..>4470
                     /locus_tag="PLQ68_10585"
                     /inference="COORDINATES: protein motif:HMM:NF025854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HXK73141.1"
                     /translation="MEKSNQSLLLFAAVPIFYYLFDYATIIYPDILYRDAKWVVQFIP
                     TVVSVFYFIFITLYYTEILKQAASQRERDMLNIHLQHSKTELTTLRQLQECTAVYRHD
                     MRHHITFLQGLASKGRIDEIKEYLKTAQSDIENITPVRFCENETVNLLLSAFSSEAKQ
                     KGIKFSIEANLLKLIPLTDTEICSLLSNSLENAIIATSKCSDPYITAKLQIHKNNLLI
                     SISNPYTGEILLKDGHPQSHYEDHGFGTRSIV"
BASE COUNT         1384 a          882 c          726 g         1478 t
ORIGIN      
        1 acgttgctta aagtaccggt atcagaacct gttgcagtaa aagtagcact tggtgtagcc
       61 tcttgtattc ctgtatacct agtgattgag ataatatgat tatttgaagc cagtgaagtg
      121 cccgctgctt ttatacgaac atagtatata ccatttgaaa ggcctgttat atcgcttcct
      181 gtcccgcttg tccattcagc agctgctact ggtttatact ccatcgctga tgtgacccct
      241 aatagtttac cgtcattgtt ggctaatgta gtacaagtag aacttgtcac agaaggtgaa
      301 aatggttgcg ttatatctat catctgtatg ctggaatcgg atgtagtagt tccatttccc
      361 agtacaaaaa cttttatatc atttgatgag tttacaccag ttatatccat cgttgttcca
      421 gttatattta tccagttgct tcctccgtca acactgtact tcatgcttgt agtcacatta
      481 ctcagagtgc ccgtatttgt accagttgct gaaaataccg cagttggagt atctaaagga
      541 ttagtaacat accatactgc aactcgctca gcccaaccgc ctgctgtcaa tgtacgaact
      601 gcatctgtat ttgttgtact aggtgaaagg ttaagagctc cattaatgga atttgcctga
      661 gctgccaaag atataccgct acctccgtta agattagtaa aagtatcact atacaatcca
      721 taactataat cttgtgaatg gcctcctaaa gcaataattt ctccatttcc tgaaagagtc
      781 atgctacctc cagctgtgtt tattccatgg cttaccgacc cagtggtatc tcctgcacag
      841 acaacatttt tcccttcaag caatatgtct gtatctgcag gtgccttaat accaaatgta
      901 tctgtagtga ttccgtctgc agatccgtta acaacaatat ttctcaatgt cagtgtattc
      961 gtgccgttat cccatgtgta tccgtcatct gctagagttt ttgtggaatg atcaccagtc
     1021 gttaaatcaa gactgcctgc taaaaccttg tataagcttg aatctccaat aacagctaat
     1081 ttcccattat aactacccga gattactcca cttatatctc tgtttaaagc acttgcattt
     1141 acagctgtag cttttgcaat gaccaaagaa ttgttgtcga tattaatatt cctgtcagtt
     1201 tgatttgcac aaattgcgcg actgttatat gttgacacag aacctgctgt agcaacaact
     1261 gtactattgc ttatggttat gtcatttctt tgggaataga taccatagct gctatggtct
     1321 cctttgccac cagacgagat aacagtacct ccatttatat taatggcacc agccatatgt
     1381 attccagctg atacccatgt tgatgatgaa cttatagcag tgaggttacc gccatttaca
     1441 gtaagagaac ctgatgaata atcattgctg ctactaatac catagctatt ccgtaatcct
     1501 gcggatccac tctttacata aaccatagct ttagtgctta tatatattgt agcactcgat
     1561 gcagctagat caatccccat actggatgaa tttgaaccgt ttaaagatcc tccgatagcg
     1621 ctcaattttg cattatcata aacatacaga ttatacaaat acagaccttc acttagtata
     1681 ttatcagttg ttatagttgg tatatctcct ccaattgcac ttactgaagc ataatttcga
     1741 attgtaactg taccggcaca atagatccca aaattcctat acgcatatga gttgtcactt
     1801 gctcttgcat caagagttac attctctgat atagtcaggt ttcccattat aaacaaccca
     1861 aaacagtaac ctgcagcaat tatatcactg gatacattca aagtaccgct tcctgatata
     1921 ttcagcagac ccttaatgta tatgccgata gcatatttat ttaagcctgt tgtaagagca
     1981 tcagggttat taacagtatt tgttccctgt aaaacaagat ttagatcacc atcagcgtat
     2041 atagcagcag tataactgct gtatgataca tcccagctat gaggcgtaga aatattagaa
     2101 ttattcaatg tcagagtata acttccactg ttgttagtaa cccaccatgt tgagtcggaa
     2161 atatcagtat cggaaaatgt tgtattacca tggcttggca atgtaacatt tgtaccccct
     2221 atattcagta tagttaatgg tactgctgcc tggacagtca tcggtataag ggcaaaaacc
     2281 atacatagta ctaacaaaac acttataact ttctttttca tgcttaaact cctgcctttt
     2341 ctttattgtt taattcatag tctgaataat atacattcaa cctaaaatcc tatttggata
     2401 tagaataata tataaaacct atttgttact tttttatgcc ttaatggtag ctattttagc
     2461 ctgaaatgca catagtcaat tgaatagtga aaagatttat tgtaaactta taacatcctt
     2521 tgagagaggc tatgaagatg ctgagaattg ctatttgtga tgacaacagt cgggacttgt
     2581 ctcatattac atcgcttgtg gaatcatata aaaaaaacaa gaaaacagat attgtttata
     2641 tgagttttca taatgctatt gatcttttgg agtcattgaa aaaaaaagaa tgtgaccttc
     2701 ttttacttga tgtattaatg ccaggtttta atggcttgca ggcagcacat gaacttcgta
     2761 aacaaaatga taaaattgaa atcatttttc ttacttcatc tcctgaatat gccgtggaga
     2821 gctacaatgt cagagcacac cattatcttc taaaacctgc tacagaagat aaacttttcc
     2881 ccttactaga caagcttatt gaaaaattaa aaaagccaga agaagcaata atagtaaaaa
     2941 gttcttccag tgttttcact attccatata aaaatattga atatgtagaa ataaatgcaa
     3001 aaacaatata tttttaccta acagataatg atgttaaaaa ggtaagtggc agtatggcag
     3061 aatacgaagc gcttttactt aaaagatctg gctttattaa agtccatcgt tcatatcttg
     3121 ttaatttgca atgggtacag cagttaaacc aaaaagaact tattacatta actggacgga
     3181 aagtacctat tgcaagaacc gtttatactc agattcgcac tgcttacact cagtttctat
     3241 tcaaggaagc agatgaatta acaaatacta aaggaggaat aaaccgtgat tgaaaccatt
     3301 actggtctta tacgctttgc cttttctttg ttctttggta ttacaatttc cgttctattt
     3361 gttggtttaa agcaaacccg gaaaaataat attacactcg cctttgtttg cacaatcctg
     3421 ttatttgccc aatatgcaag tctatggctt tttggtttgg agttaacctc aaaattatat
     3481 ccgtttatta ttcatatacc gatgatcctg cttttttctt tgtactttaa acgaccctgg
     3541 cttatatcca cagtaagtgt aatttctgct tatttatgct gccaggctcc acgctggatt
     3601 ggttttattg caggtgatgc tttcaagagt aacattgcag accatttttc atatgtcata
     3661 tcaataatac ttgcttactt tttacttaaa aaatatgttg caaactcagt aagacaactt
     3721 atggagaaat caaatcaatc cctcttactt tttgccgctg ttcccatttt ttactattta
     3781 ttcgactatg ctactattat ttatccagat atactttaca gggatgctaa atgggtagtt
     3841 cagtttatac caactgtagt atctgttttc tactttatat ttataactct gtactatact
     3901 gagatcctaa aacaagcagc ttctcaaaga gaacgtgaca tgctgaatat tcatctacag
     3961 cattctaaaa cagagcttac tactttacga caattacagg aatgtactgc tgtttaccgt
     4021 catgacatga gacaccatat tacatttctt cagggacttg catctaaagg acgaattgat
     4081 gagataaagg aatatcttaa aacagcacaa tctgatatcg aaaatattac tccagtacgt
     4141 ttttgtgaaa atgaaactgt caatcttctt ctgtcagctt tttcttctga agcaaaacaa
     4201 aagggaatta agttttccat agaagctaat ttgctaaaac ttattccgct taccgatacg
     4261 gaaatttgtt ctttactctc aaatagcctt gaaaatgcca ttattgcaac atcaaaatgc
     4321 tctgaccctt atataaccgc taaactgcag atacacaaaa acaatctttt aatctccatt
     4381 agtaatcctt atacaggaga aatattacta aaagacggac accctcaatc gcattatgaa
     4441 gatcatggtt ttggtacacg aagcattgtg
//
LOCUS       DAOSXM010000126         4465 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2111
            NODE_13245_length_4465_cov_10.504762, whole genome shotgun
            sequence.
ACCESSION   DAOSXM010000126 DAOSXM010000000
VERSION     DAOSXM010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864178
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4465)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4465)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:24:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,261
            CDSs (total)                      :: 2,229
            Genes (coding)                    :: 2,218
            CDSs (with protein)               :: 2,218
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 3 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4465
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13245_length_4465_cov_10.504762"
                     /isolate="AMR_MDS_2111"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            57..839
                     /locus_tag="PLQ68_10590"
     CDS             57..839
                     /locus_tag="PLQ68_10590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008413382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HXK73142.1"
                     /translation="MKVIFIGDIYGKTGRIALKNNMSLLKDKYSPDFVIANGENTDKG
                     KGITPTLLDELLSFGVDVVTSGNHIYAKKEILIPLEGNIPLLRPINYSKACPGKGYMI
                     LSKNGVSLAVINVSGRVYMDPCDNPFDAVEEILPEIKKKVKNIIVDFHGEATSEKTAF
                     AWHFDGRVSAVIGTHTHVQTADERILPFGTGFITDAGMTGPYEGIIGASRDKVIDRFL
                     TGMPAYFSAQEGIGQINGVFLDLCENTGKTLHIERIYYVEKD"
     gene            823..1302
                     /gene="lspA"
                     /locus_tag="PLQ68_10595"
     CDS             823..1302
                     /gene="lspA"
                     /locus_tag="PLQ68_10595"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019242383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HXK73143.1"
                     /translation="MLKRISAKNLLWLFLSIFLIAADQVTKLIITSNMKIGDSITVIE
                     DFLYIGYTRNTGAAWGIFGGHTEYLAIFSIIASIALIYLLLTFRRKFAAFSTALVIGG
                     AIGNAIDRIRLGWVIDFVDTYIFGYDFPMFNVADSAITVGVILLVIYLLFIHREMQT"
     gene            1302..2213
                     /locus_tag="PLQ68_10600"
     CDS             1302..2213
                     /locus_tag="PLQ68_10600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008420758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HXK73144.1"
                     /translation="MDSNYVLHVNQDEGLRLDTYLAKYIEDYSRSYIQKTIKDGNVTV
                     NGKIKNPSYKVNSKDSIMVTISSNNPSSIEPEDITLDIVYEDDDIIVVNKSRGMVVHP
                     AIGNYSSTLVNALMYHTKNSLSDINGIERPGIVHRIDKDTSGVIVVAKNNSSHRFLAD
                     IFSRHDIKREYIAIVKGYPDSEKGKIDAPIGRDKFNRLKMSVESVAGKRAVTYFEVKQ
                     KLDGASYISAFLETGRTHQIRVHMSFIGNPLLGDDIYGGKDKRFTNGQLLHARLLGFV
                     HPRTHQYMEFVAEPDQYFENALEVLSK"
     gene            2210..2899
                     /locus_tag="PLQ68_10605"
     CDS             2210..2899
                     /locus_tag="PLQ68_10605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013238790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HXK73145.1"
                     /translation="MNDYEIHERTISLIGEEAFEKLTNAKVVLIGLGGVGSYILEALA
                     RAGIGHLVVADFEKIELSNINRQLLALHSTLGHKKTDCAYNRVKDINPNIEVKCYDIF
                     VNKENISDIVKDDTDYIIDAIDSVQSKIDIIKYSKEKGIRIISCMGTGNKTDNTCFKI
                     ASIKKTDTCPLAKKIRKILSSEGISDVDVLFSSCSNEKKISGAIPSISYVPSTAGLLI
                     AGYVIQKLIEE"
     gene            2913..4442
                     /gene="xylB"
                     /locus_tag="PLQ68_10610"
     CDS             2913..4442
                     /gene="xylB"
                     /locus_tag="PLQ68_10610"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - xylulokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HXK73146.1"
                     /translation="MNYLLGIDIGTSATKSVLFDEFGKAICSASYEYPMYQPNTGWAE
                     QDANDWWKAVCHTCKKILSDSGIDNKQISAVGLSGQMHGLVMLDEKDNLLGKSIIWCD
                     QRTEQQAVFLEDKIGRDKLISITANPAITGFTASKILWVKQNNPKLYEKAKKIMLPKD
                     YIVYMLTGEFSTEVSDASGMQLMDIKKRQFSKELLDKLEIDISLLGYMHESSDAAGRI
                     SKKAAELTGLDEGTIVAAGGGDQACGAVGNGIVKEGIVSSTIGTSGVVFAHTDNLHID
                     KYGRIHTLCHAVPGCYHVMGVTQAAGLSLKWFRDSFCSEQIKQAEKEGVDPYLIMDKM
                     AEKIQIGAQGLIFLPYLMGERSPHLDPFCSGVFFGITPIHEKAHFIRAILEGVVYSLK
                     DSISILRNDMKVDISQVRVSGGGAKSSLWKSMQADIFESDVAMTDNAEGPALGAAILA
                     GVAKGLYKSVPAACEHVITTKNVHKYDMNKANQYNKYYEIYKSLYPVLKNEYRKLRKL
                     L"
BASE COUNT         1574 a          610 c          924 g         1357 t
ORIGIN      
        1 ttacttctca aaaacatatt attagtctat aattaaataa atagaaagag gtaatagtga
       61 aagttatatt tataggtgat atatatggaa agacagggcg catagcttta aaaaataata
      121 tgtctttgct gaaagataaa tattcacctg actttgtaat tgcaaatggg gaaaatactg
      181 acaaaggaaa aggtattaca cctactttgc tggatgagtt gctgtctttt ggtgtggatg
      241 tggttacttc aggaaatcat atatatgcaa aaaaagaaat actaatacct ttggaaggga
      301 atattccgct tcttcggcct attaattatt caaaagcttg tcctggaaag ggttatatga
      361 ttttaagtaa aaatggagtc tcattagcag ttattaacgt ttcgggaaga gtgtatatgg
      421 acccttgtga taatcctttt gatgcagttg aagaaatatt acctgaaata aaaaagaaag
      481 taaaaaacat cattgttgat tttcatggag aagcaacgag cgagaaaact gctttcgcgt
      541 ggcattttga tggcagagta tcagctgtta ttggtaccca tacacatgtt cagacagctg
      601 atgagagaat acttcctttt ggtactggat ttataactga tgcaggaatg actggccctt
      661 atgaaggaat aatcggagca tcaagagata aggttataga taggttctta actggaatgc
      721 ctgcatattt ttcagctcag gaaggaatag ggcagataaa tggtgttttc ttggatcttt
      781 gtgaaaacac tggaaaaaca cttcatattg aaaggatata ttatgttgaa aaggattagt
      841 gcaaaaaatc tgttatggct ctttttaagt atttttttaa tagctgctga tcaagtaaca
      901 aaactgatta ttacatctaa tatgaaaata ggtgacagca ttacagtaat agaagatttt
      961 ctttacatag gatacacaag aaatactgga gcagcatggg ggatttttgg aggtcatact
     1021 gaatacttag ctattttctc aattattgca tcaattgctt taatatacct tttattgaca
     1081 ttcagaagga agtttgcagc tttttcaaca gctcttgtta tcggaggagc aattggaaat
     1141 gcaatagaca gaattcgtct tggatgggtt atagattttg ttgacacata tatattcggt
     1201 tatgactttc ctatgttcaa tgttgcagat tcagcaataa ctgtcggagt tatactactg
     1261 gtaatatatc tgctttttat tcatagagag atgcagacat gatggacagt aattatgttt
     1321 tacatgtaaa tcaggatgaa ggattaaggc tggatacata tctagccaaa tatattgaag
     1381 actattcacg ttcatatatt caaaagacaa tcaaagatgg aaatgttact gttaatggca
     1441 agataaaaaa tccaagctat aaagtaaata gtaaagacag cataatggtt actatttcaa
     1501 gtaataaccc cagttcaatt gaaccagaag acataacttt agatattgta tatgaggatg
     1561 atgatattat cgtagtaaat aagtcaagag gaatggttgt acatcctgct attggtaatt
     1621 attcatcaac tctagtaaat gctctgatgt atcatacaaa gaatagttta agcgatatta
     1681 acgggattga gagacctggt atagttcacc ggatagataa ggatacatca ggagtaattg
     1741 tagttgcaaa aaataacagt tctcatagat ttttagcaga tatattttcc agacatgaca
     1801 taaaaagaga atacatagct atagttaagg gttatcctga ttctgaaaaa ggaaaaattg
     1861 atgcccctat aggaagagat aaatttaaca ggcttaagat gtctgtggaa tcagttgctg
     1921 gtaaaagggc agtaacttat tttgaagtaa agcagaaact ggatggagca tcatatatat
     1981 cggcttttct tgaaactgga agaactcatc agataagggt acatatgtca tttataggta
     2041 acccactttt aggagatgat atatatggag gaaaagacaa acgattcaca aatggccagc
     2101 ttctacatgc aagactatta ggttttgttc atccacgaac gcatcaatac atggagtttg
     2161 tagcagaacc agatcagtat tttgaaaatg cgctggaggt tttatccaaa tgaatgatta
     2221 tgagattcat gaaagaacaa taagccttat aggtgaagaa gcgtttgaaa agcttactaa
     2281 tgcgaaggta gttttaattg gccttggtgg cgtgggctct tatatattag aagctttggc
     2341 aagagctgga ataggacatt tagtagtagc tgatttcgaa aaaatcgagt tatcaaatat
     2401 caacagacag ctacttgcat tacactccac ccttggacat aaaaagactg attgcgcata
     2461 caatagggtt aaagatataa atcctaatat agaagtaaaa tgttatgata tatttgttaa
     2521 taaagaaaat atttcagata ttgttaaaga tgacacagac tatataattg atgctataga
     2581 ttcagtacaa tccaaaatag atataattaa atattcaaaa gaaaagggta taaggattat
     2641 atcttgtatg ggcacaggca acaagactga taatacctgt tttaagattg caagtataaa
     2701 aaagactgat acatgtccat tagcaaaaaa gataagaaaa atattaagca gtgaaggtat
     2761 ttcggacgtg gatgttcttt tttcatcatg ttctaatgag aaaaagatat ctggtgctat
     2821 acccagcatt tcttatgttc cttctacagc aggacttctt atagcgggat atgttataca
     2881 aaaactgatt gaagaatagg agaagatata ttatgaatta tttattagga atcgatattg
     2941 gaacatcagc aacaaaatca gtactatttg atgaattcgg aaaggccatt tgttccgcat
     3001 catatgagta tcctatgtac cagcctaata cgggctgggc agaacaggat gcaaatgact
     3061 ggtggaaagc tgtttgtcat acatgtaaga aaatattgtc tgattcaggg attgataata
     3121 aacagatatc agctgtaggc ctgtcaggtc agatgcacgg gcttgtcatg cttgatgaaa
     3181 aagacaatct tttaggaaaa tctataatat ggtgtgatca gagaactgaa caacaagctg
     3241 tatttttaga agataagatt ggaagagata aactcatatc cattaccgct aatcctgcaa
     3301 taaccggttt tactgcttca aaaattctat gggtaaaaca gaataatcca aaattatatg
     3361 aaaaagctaa aaaaataatg cttcctaagg actatatagt ttacatgctt acaggcgaat
     3421 ttagtacaga ggtatctgat gcatctggaa tgcagcttat ggatataaaa aaaagacagt
     3481 tttcaaaaga attgcttgat aaattggaaa tagacatatc attgttaggg tatatgcatg
     3541 agtcaagtga tgctgcaggc aggatatcaa aaaaagctgc tgaacttaca ggtcttgatg
     3601 aaggtaccat tgttgcagca ggaggcggag atcaggcttg cggggctgtg ggaaatggta
     3661 ttgttaaaga aggtatcgta tcatcaacga taggtaccag tggtgttgtt tttgctcaca
     3721 cagataatct tcatattgat aaatatggaa gaatacatac cttatgccat gcagtaccag
     3781 gttgttatca tgttatgggt gtgacccaag cagcgggatt atcgcttaaa tggtttagag
     3841 acagtttttg tagtgagcaa attaaacaag cagaaaaaga aggcgttgat ccatatttga
     3901 tcatggataa gatggcagaa aaaattcaga taggagcaca gggtttgata tttcttccat
     3961 atcttatggg tgaaagatca ccacatctgg atccattttg ttcaggggtg tttttcggta
     4021 ttactcctat acacgaaaaa gctcatttca taagagccat tcttgagggt gtcgtatatt
     4081 cacttaagga cagcataagc atattaagaa atgatatgaa agttgatata tctcaggtaa
     4141 gagtatccgg aggaggagca aaaagctctt tgtggaaatc aatgcaggct gatatctttg
     4201 agtctgatgt ggcaatgaca gataatgctg aaggtcctgc tttaggtgcg gctatacttg
     4261 caggtgtagc aaaagggtta tataagtctg ttcctgctgc ttgcgaacat gtaattacaa
     4321 ctaaaaatgt tcataagtat gacatgaata aggctaatca atataataag tattatgaaa
     4381 tatataaatc actttatccc gttttaaaga atgagtatag aaaattaagg aaactattgt
     4441 aatagtttga ttgtatttac aacat
//
LOCUS       DAOSXM010000127         4465 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2111
            NODE_13246_length_4465_cov_10.497052, whole genome shotgun
            sequence.
ACCESSION   DAOSXM010000127 DAOSXM010000000
VERSION     DAOSXM010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864178
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4465)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4465)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:24:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,261
            CDSs (total)                      :: 2,229
            Genes (coding)                    :: 2,218
            CDSs (with protein)               :: 2,218
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 3 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4465
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13246_length_4465_cov_10.497052"
                     /isolate="AMR_MDS_2111"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..80)
                     /locus_tag="PLQ68_10615"
     CDS             complement(<1..80)
                     /locus_tag="PLQ68_10615"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HXK73147.1"
                     /translation="MIKNKKGITLVELLAVIVILAIITAIA"
     gene            complement(186..1406)
                     /locus_tag="PLQ68_10620"
     CDS             complement(186..1406)
                     /locus_tag="PLQ68_10620"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HXK73148.1"
                     /translation="MADQNYSYTAKNTQGKIVKGKIYAVNRYICIKYLESKNLQVQKL
                     TEFKSYYSKLSQVAIGRVLKPKQLVFFLRQFGVLLESGVMLLDALEMLLLQEEKIIMR
                     KLFLDIYEHVYSGYSLSQAFATKPNDFPKYLVSMIEVGEMSGKLSPMILEMAEYYEKQ
                     FKITREIKSAIRMPIIYLIATVLIAAGMVLFVFPSISSLYASFGDTKLPGITLFFLNA
                     SDFVAKNIFAFAGIIIGITVLLILLIKYSPKARYEMTKAALQLPIIGGLIQMNNQIII
                     TNTLSQMLGSGVHSTKSLATLKTVLTNDVYKQIVDQTVKYIEEGLPFSRAFQESPFID
                     TVLAKMIASGEKAGDLPGLMKNMAEYYNTISDMKVSKLKDSIQPILLIVIYAIVGVMI
                     LALMLPMLALGTQI"
     gene            complement(1417..3057)
                     /locus_tag="PLQ68_10625"
     CDS             complement(1417..3057)
                     /locus_tag="PLQ68_10625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013489677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HXK73149.1"
                     /translation="MALLRIGDILTQEKIITEDQLKKALKEKQSDERLGMTLIRLGYA
                     TEGQILKALENSTGVQRVSLSKYSYDDKVFELVDEDFCTRNNIIPLKIEGKNLHFATS
                     DPLDFAVFEDLRVITGLRPVAFSATRREIETEIAKYYGFSRTLDALKTTQSVVEQEIT
                     FDPTPMVQLVNQLLTSAVFQRASDIHIDPLEDRIAVRYRVDGALDTVRELPANIKNQI
                     TSRVKIMADMDITESRIPQDGRIQTTINGRDIDLRISTLPTVNGEKIVMRILDLSNNI
                     NNISYLGLSAEGEKKIRKMIKRPNGIVLVSGPTGSGKTTTLYACLNELNRPPVNIITV
                     EDPVEIRMEGINQVQVRQNVNLTFANALRSILRQDPNIIMIGEIRDIETAEIAVRASL
                     TGHLVLSTIHTNDAVKTITRLLDMKIEPFLIASSLTGVVSQRLVRRLCEECMTESNAT
                     ASEKEYFARYGMEVEKLKRAKGCPSCNFKGYAGRTGVYEILEINETMRTLISEKASIV
                     ELEKEAKNQGMKSLMESCLEAVKEGITTLEEVMKVAHD"
     gene            complement(3059..4246)
                     /locus_tag="PLQ68_10630"
     CDS             complement(3059..4246)
                     /locus_tag="PLQ68_10630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK73150.1"
                     /translation="MKEWVRKSGYIYLSLSILILAVLFASAMVSLFWTEEKASNTTVG
                     TVYLGDKSAQDYEISLLLGISEWKEKANFRISYNEYERKIPLDIFEFDVDTTIFNLVE
                     NQKNLAIFSLSEPYFSEFKTDIENYFGPVIYEALDVEKLFNDILYDAEKMSLQKTYTL
                     YSYLNDELSKTSLNISKISNIRSYDINEIVSLANEIHIPANERFSLLDTIGSVNLSNE
                     QMSIIASGIQSVIMPTSFSGIISQQYLDPPIWSESGKNVRILQVSKIDFSFFNPIDTD
                     YIVKIEKKGINTLEFELIGFPFISEFAIESVLDNEIAYQTEYVEDITIGPDTKNVIII
                     EQEFDTVYRILEQKGQSGSLTIFYRTITHPDGSSETSSILQEFVNPQNRIYRQNTVDK
                     EDN"
BASE COUNT         1361 a          837 c          647 g         1620 t
ORIGIN      
        1 gctattgctg ttatgattgc aagtataact attactgcta ataactctac cagtgtaata
       61 cctttcttgt tctttatcat cattttcata atacccctct tttccacatc tagtttatgt
      121 aattattata ccatacaatt atttaaatca atagatttca tgtaaaattt gtaaatatta
      181 aatttctata tctgagtacc taaagcaagc attggaagca taagagcaag tatcattacg
      241 cctacaatag catagattac tatcagtaat atcggttgga ttgaatcttt cagttttgaa
      301 accttcatat cagatattgt attatagtat tcggccatgt tcttcattaa tccaggaaga
      361 tcaccagctt tctctccaga tgcaatcatc tttgcaagaa cagtatctat aaatggactt
      421 tcctggaaag ctcttgaaaa aggtaatcct tcttcaatgt atttgactgt ttgatcaact
      481 atctgcttat atacatcatt tgttaataca gttttaagcg ttgctaatga tttagtcgaa
      541 tgaacaccgc ttcctaacat ctgagataat gtatttgtta tgattatctg attattcatt
      601 tgaataagtc caccgataat tggtaactgc aatgctgctt ttgtcatttc ataacgagct
      661 ttgggtgagt attttatcaa taatattaaa agaactgtta ttccgataat tattcctgca
      721 aatgcaaaaa tatttttagc aacaaagtca cttgcattca aaaagaaaag agttattcct
      781 ggaagcttag tatcaccaaa ggaagcatac aaggaactta tgcttggaaa tacaaacaaa
      841 accatacctg ctgcgataag aacagttgca ataagatata ttattggcat tctgattgcg
      901 cttttaattt ctcttgttat cttaaattgt ttttcgtaat attctgccat ttccagaatc
      961 attggtgaga gctttcctga catctcccct acttctatca tgctgacaag gtatttagga
     1021 aaatcattcg gtttagttgc aaacgcctga gaaagagaat acccgctgta tacatgctcg
     1081 tagatatcta aaaaaagctt cctcattata attttctctt cctgtaataa aagcatctcc
     1141 agtgcgtcta aaagcataac tcctgattca agcagtactc cgaattgacg aagaaagaat
     1201 accagttgct ttggctttaa taccctgcct atagcaacct gacttaattt agaataataa
     1261 ctcttaaatt ctgtgagctt ttgaacctga aggtttttgg attcaaggta ctttatgcat
     1321 atatatctgt taactgcata aattttaccc ttaactattt ttccctgagt atttttagct
     1381 gtatatgaat aattttgatc agccatactt tatcccctaa tcatgtgcta ctttcattac
     1441 ttcctctagt gttgttatac cttctttaac agcttctaga catgattcca tcagtgactt
     1501 catcccctga ttcttagctt ctttttccaa ttcaactata ctggcttttt cagatataag
     1561 agtacgcatt gtttcattta tttccagtat ctcatacact ccggttctac ctgcataacc
     1621 tttaaaatta catgaaggac agcctttagc tcttttgagt ttttctactt ccataccgta
     1681 tcgtgcaaaa tattcttttt cacttgctgt cgcattactt tcagtcatac attcttcgca
     1741 taagcgtctt accaatcttt gtgaaacaac gcctgttaat gaagaagcaa ttaaaaatgg
     1801 ctctattttc atatcaagaa gtctggttat agtcttaaca gcatcgttag tatgtattgt
     1861 gcttaaaaca agatgaccag tcagagaagc tcttactgca atttctgcag tttcaatatc
     1921 ccttatttct cctatcataa taatattagg gtcctgtctt aaaattgaac ggagagcatt
     1981 tgcaaaagtc agattcacat tctgccgtac ctgcacctga tttatgcctt ccattcttat
     2041 ttctactgga tcttctacgg ttattatatt taccggaggt ctgttcaatt catttagaca
     2101 tgcatataga gtagtagttt ttccagaacc tgtaggtccg ctgactaaaa ctataccatt
     2161 aggccgtttt atcattttac gtatcttttt ttcaccttca gcgctcaatc caagatagga
     2221 tatattgttt atattattcg ataggtcaag aattctcata acaattttct cgccattaac
     2281 agttggcaaa gttgaaatac gaagatcaat atctctgcca ttgatagttg tctgaattct
     2341 accgtcttgc ggaattctac tttcagttat atccatatct gccattattt ttactctgct
     2401 tgttatctgg ttttttatat ttgcaggcaa ttcacgtaca gtatccaaag caccgtcaac
     2461 cctgtatctt acagctattc tatcttctaa cgggtctatg tgaatgtcag aagctctttg
     2521 aaaaacagct gatgtaagta gttgattaac cagttgaacc attggtgtag gatcaaatgt
     2581 aatttcctgt tcaacaacag actgtgtggt ttttaaggca tcaagcgtcc ttgaaaatcc
     2641 gtaatattta gctatctcag tttcaatttc tcttcgtgta gctgaaaaag caactggcct
     2701 taaacctgta attactctca agtcttcgaa aactgcgaaa tccagtgggt ctgatgtagc
     2761 aaaatgaagg tttttccctt caattttcaa tggaattata ttattcctgg tacaaaaatc
     2821 ttcatcaaca agctcaaata ctttgtcatc ataactatat ttggataaag atactctttg
     2881 tactccggtt gaattttcaa gagcttttaa tatctgtccc tcagttgcat aacctaaacg
     2941 tatcaaagtc atacctagtc tttcatcaga ctgtttttct ttaagcgcct ttttcagctg
     3001 atcttctgtt atgatttttt cctgtgttaa gatatcaccg attcttaata aagccatatt
     3061 aattatcctc cttatccaca gtattctgtc tatatattct attctgagga tttacaaatt
     3121 cttgtaatat tgatgaggtc tcagatgaac catctggatg agttattgtt ctatagaaaa
     3181 tagtaagaga tcctgactgc cccttttgtt ctagtatccg ataaacagta tcaaattcct
     3241 gttcaattat aatcacattt ttagtatctg gacctattgt aatatcttct acatattctg
     3301 tctgataagc aatttcatta tccaaaacag actctatagc aaattcactt ataaatggaa
     3361 accctatgag ttcaaactca agtgtattaa tacctttttt ttcaatttta actatataat
     3421 cagtatcaat aggattaaaa aaagaaaaat caattttact gacctgaagt attcttacat
     3481 ttttccccga ttcagaccat ataggggggt caagatattg ttggctgatt ataccggaaa
     3541 aacttgttgg cattataaca gactgaattc ccgatgcaat aatactcatc tgctcatttg
     3601 aaagattaac cgaaccaatt gtatccagca aagaaaatct ttcatttgcc ggaatatgta
     3661 tttcatttgc taaagatact atttcattaa tgtcatatga acgtatattt gatattttcg
     3721 aaatatttaa tgatgttttc gatagctcat catttagata tgaataaaga gtatatgttt
     3781 tttgaagtga cattttctct gcatcatata aaatgtcatt aaaaagtttt tctacatcaa
     3841 gagcttcata aatgaccggt ccaaagtaat tttcgatatc tgtcttaaat tctgagaaat
     3901 aaggttcaga taaactgaat atggccagat tcttctgatt ttccactaaa ttaaaaatag
     3961 tcgtgtcaac atcaaattca aagatatcta aagggatttt tctttcatac tcattatagc
     4021 tgatacgaaa gtttgctttt tctttccatt cactaatacc aagcaaaaga gaaatttcgt
     4081 aatcctgagc agacttatct cctagatata ccgtaccaac cgttgtgttg cttgctttct
     4141 cttcagtcca gaataaagaa accattgcag atgcaaaaag gacagcaaga attaaaatac
     4201 tcaaagataa ataaatatac ccgcttttac gtacccattc tttcattctg ttctacccct
     4261 tgtaaccctt ttcttcttaa ttatatcata actaaatatt aatctacctg attttaacgt
     4321 atacttacat ttttttgatt ttctttatta attcattgta taattaagcc ataatacaag
     4381 gaggtgcatt aatgaatcgc cgtggagtat ccttaatgga ggtagtggtt ggaatatttc
     4441 tgatttcact cgtagtttta agcgg
//
LOCUS       DAOSXM010000128         4456 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2111
            NODE_13286_length_4456_cov_9.001136, whole genome shotgun sequence.
ACCESSION   DAOSXM010000128 DAOSXM010000000
VERSION     DAOSXM010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864178
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4456)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4456)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:24:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,261
            CDSs (total)                      :: 2,229
            Genes (coding)                    :: 2,218
            CDSs (with protein)               :: 2,218
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 3 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4456
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13286_length_4456_cov_9.001136"
                     /isolate="AMR_MDS_2111"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..243
                     /locus_tag="PLQ68_10635"
     CDS             <1..243
                     /locus_tag="PLQ68_10635"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HXK73151.1"
                     /translation="LVKTKRTYNQASLNFVNRMNNLKDVFQAHDNLSAGRKIIVVDDI
                     WTTGATLKECEKVLLNNKASSVILVTISKNKKKQIY"
     gene            301..1677
                     /locus_tag="PLQ68_10640"
     CDS             301..1677
                     /locus_tag="PLQ68_10640"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HXK73152.1"
                     /translation="MVSSERYKILKLLQKNTKITRYLVFDEIDKCKKVISETKKKLNS
                     EDMIKDTLKIDYPGLPRITDIFISGENHCTVCEYVEGESVFDYINEKGVLDIYESIDI
                     FLKILKMIKFLHEIKPEPIIHNNIHHDNVILIDEEVYLIDYGIKDEKKNPYIAPESII
                     GNSENKQQDIYSLGILLNYMITGNFKKPQYSNKQQSSIDILISRCTMLNLNKRFDDVG
                     VVINECNRILNKIKAEEFEDYTPLYATIGIHKTLFKMVKLFSEQKDKSIINLRSKKVL
                     IIDTDLLHPSFNPNEYVLNESFQKLIDREDKVPLKPAVKGTEVYIIPCDLIIENYENI
                     VFTQLEKVISDYAEDFDAIFIKCSDFIYDSLTVNSIMIADQTIFFMNNPLYEIKLFNS
                     ISRYLCSRHNMDKKRFNFIMDKSDSKKEIVTNIEAHVYGDYLGTLSSKKRWYNNQYKP
                     INLLKSKV"
     gene            1680..2870
                     /locus_tag="PLQ68_10645"
     CDS             1680..2870
                     /locus_tag="PLQ68_10645"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HXK73153.1"
                     /translation="MAVLKSLNTISDNSDNVKYEKGSYSLSKIVTEVTNELLTDSAEL
                     VTNAYYDKKQRSCLEKRILYILDNQQYFAVGSKRKMIKSIFDFMFGYGQLQEYIEDDE
                     ISDIDGVRHNCFSITKNGIRIPIDIDFNSESEFNVYCKLIALRNGGILNENDNHCRVA
                     DLENKLRINLSIYPRNISGPAISIRKHRQIAYNMDDLVRLKMLDTTCKELITELLLND
                     KTIILCGKGGSGKTTLLRALINILPKMERVLIVESDAELYPDKANCIEQRVKRDNEGG
                     NPITLEMLIKDGLTMSLDTYCIGEIVGNESMAFLNACCCGHRFLTTMHSNKPQDSASR
                     LIALAKRAVGTTDEKMLTWMFCEGVDAIIHMNKFKVMEIVCVKGYDSSNQKPEYDLLY
                     RRNV"
     gene            2867..3661
                     /locus_tag="PLQ68_10650"
     CDS             2867..3661
                     /locus_tag="PLQ68_10650"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HXK73154.1"
                     /translation="MIYLGINIVIFILFFFIQKSKYHYEDLKILMIRKMDYLVIGLSL
                     ILYIAISLIVNTKINNLLTSFTVSLMPSSLPYLMFDVYKNSLLKKEKEGITGLLMILT
                     KWSSVKNDAVYCLQKANESNLKNPIGKYIKNLCIRLEKGMSVSDSLISCEKDALSEEL
                     RYVFINVRYAYEKGGSLYSIFKNLENQFFKIDEENYKRKISTSTDKYAVYISIILVMF
                     TFYLIVLNNDYSKKYYFNSTLGIYILGIFSLLFFIGVLIMAKKVRE"
     gene            3665..4456
                     /locus_tag="PLQ68_10655"
     CDS             3665..4456
                     /locus_tag="PLQ68_10655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK73155.1"
                     /translation="MDSITIYMLFIGNIAVGILFSAHIVSMRIAESVNDGKITFLSEI
                     YQKIKKTVSKITYVSTEKAGEFVMICSFFLVICILVIISSGFLKAFVVLMLCLFFLYL
                     TKRKWEKDLCDIFQKNAYKLYRYLVSQIQAGVRPKDAILNMHKITEDKQLNSILAKAS
                     AAYAITFDGKTYANVLKERIKTQDAQRFCMIFEEDLLYTDNENFLEKMEEMMFNRHFS
                     YHQKKTEKVKRNCFATVCVFMLIVVIMVVLPLFSELVQGLNNIFN"
BASE COUNT         1729 a          504 c          757 g         1466 t
ORIGIN      
        1 cttgttaaaa ctaaaagaac atataatcag gcatcgttaa attttgtaaa taggatgaat
       61 aatctcaaag atgtttttca agcacatgat aatttatcag caggtagaaa aataattgtt
      121 gttgatgaca tatggactac tggagcaact ttaaaagaat gtgaaaaagt tcttttaaat
      181 aataaagcaa gtagtgtaat tcttgtaact atatcaaaaa acaaaaaaaa gcaaatatat
      241 taaaattgta tattgacaat aaatgtaaat agtatatatt tatcttcaca aggaggatac
      301 atggtttcca gtgagagata taaaatacta aaactacttc aaaaaaatac taagattaca
      361 agatatttag tatttgatga aatcgacaaa tgtaaaaaag ttattagtga aacaaaaaag
      421 aagttgaatt cggaagatat gattaaggat actttgaaaa ttgattatcc tggacttcca
      481 aggataacag atatttttat aagcggggag aatcattgta cagtttgtga atatgttgaa
      541 ggagaatcag tatttgatta tattaatgaa aagggagtac tggatattta tgaatctata
      601 gatatatttc ttaagatact taaaatgata aaatttcttc atgaaattaa gcctgagcct
      661 attatacata ataatattca tcatgataat gttatattaa ttgatgaaga agtttatctg
      721 atagattatg gtataaaaga tgaaaaaaag aatccatata ttgcaccgga atcaataata
      781 ggtaattctg aaaataaaca gcaagatatc tattcactag gtattcttct aaattacatg
      841 ataactggaa attttaagaa accacaatat tcaaataaac aacaaagctc cattgatatt
      901 ttaataagca gatgcactat gcttaatctt aataaaaggt ttgatgatgt tggtgtagta
      961 attaatgagt gtaatagaat actcaacaaa attaaagcag aagagtttga agattatacg
     1021 ccgctttatg caacaatagg tatacataaa acactattta agatggtgaa acttttcagt
     1081 gagcaaaaag ataaatcaat aattaattta agaagtaaga aggtgctgat aattgatact
     1141 gatttattgc atccttcatt taacccaaat gagtatgttt taaatgaaag ttttcaaaaa
     1201 ttaatagata gagaagataa agtaccactt aagccagctg tcaaaggaac agaagtatat
     1261 attattccat gtgatttgat tattgaaaat tatgaaaata tagtatttac acaattagaa
     1321 aaggttattt cagattatgc agaggatttt gatgctatat ttattaaatg ttcagatttt
     1381 atatatgatt cattgactgt aaacagcata atgattgcag atcaaacaat cttttttatg
     1441 aataatcctt tatatgaaat caaactattt aattcaatta gcagatattt atgttcaagg
     1501 cacaatatgg ataagaagag gtttaatttc attatggata aatctgattc taaaaaggaa
     1561 atagtaacta acatagaagc tcatgtctat ggtgattatt taggaacgtt atcatcaaaa
     1621 aaaagatggt ataacaatca atataaacca ataaatttat taaaatcaaa ggtgtagata
     1681 tggcagtatt aaaaagttta aatacaatat cagataattc agataatgtt aagtatgaaa
     1741 aaggaagtta ctccttatca aaaattgtca cagaagtaac caatgaatta ttaacagatt
     1801 cagctgaact tgtgactaat gcatattatg acaaaaaaca aagaagttgc cttgagaagc
     1861 gaatcttata tattctcgat aatcagcaat attttgcagt tggctcaaaa agaaagatga
     1921 ttaaaagtat atttgatttc atgtttggat acgggcagct acaggagtat atagaagatg
     1981 atgaaatttc agatattgat ggagttaggc ataactgttt ttcaatcaca aaaaatggaa
     2041 tcagaattcc aattgatata gactttaaca gcgaatctga gttcaatgta tattgtaaat
     2101 tgattgcatt aaggaatggt ggaatattaa atgaaaatga taatcactgc agagttgcag
     2161 atttggaaaa caaattacgt attaatttgt caatataccc aagaaacatt tcaggaccag
     2221 caatatcgat aagaaaacat cgtcagattg cgtataacat ggatgattta gtaagactca
     2281 aaatgttaga tactacctgt aaagaactaa taacagaatt actcttgaat gataaaacaa
     2341 taatattgtg cggcaaaggg gggtcaggta aaaccactct gttaagagct cttattaata
     2401 ttctcccaaa gatggagaga gtacttatag tggaatctga tgcagaactt tatcctgata
     2461 aagctaactg tatagaacaa agagttaaaa gagataatga aggaggaaat cctattactc
     2521 ttgaaatgct cataaaagat ggtttgacta tgtctttaga tacatactgt ataggtgaaa
     2581 tagttggtaa tgaatcaatg gcttttctta atgcatgctg ttgtggacat agatttttaa
     2641 ctacaatgca ttccaacaaa cctcaagatt ctgcttctag gcttatagcc ttagctaaaa
     2701 gagcggttgg tacaactgat gaaaaaatgc tgacatggat gttttgcgaa ggtgttgacg
     2761 caataataca tatgaataag tttaaagtta tggaaattgt atgtgtaaaa ggttatgatt
     2821 catcgaatca gaaaccagaa tatgaccttt tatacaggag gaacgtatga tttatctagg
     2881 aataaatata gtaatattca tattgttctt ttttatccag aaatctaaat atcattatga
     2941 agatttaaag attcttatga ttagaaaaat ggactactta gtgataggtc tttcactaat
     3001 actatatata gcaatatcac tgattgtaaa cacaaaaatt aataatctgc ttactagttt
     3061 tactgtttcg ttaatgccat catcgttacc ttatcttatg tttgacgtat ataaaaacag
     3121 tttactgaaa aaggaaaaag aaggaattac tggactgtta atgattttaa caaaatggag
     3181 ttcggttaag aatgatgctg tgtattgtct tcaaaaagca aatgaatcta atttaaagaa
     3241 tcctatagga aaatatataa aaaacttatg tatcaggctt gagaagggaa tgagtgtatc
     3301 agattcactt atatcatgcg aaaaagatgc acttagcgaa gaattaagat atgtttttat
     3361 taatgtaagg tatgcttacg aaaagggagg aagcttatat agtattttca agaatctgga
     3421 aaaccagttc tttaaaatag atgaagagaa ttataaaaga aaaataagta catctacaga
     3481 caagtatgct gtatacattt caataatatt agtcatgttt actttttatt taattgtttt
     3541 aaataatgat tattcaaaaa aatattattt caactcgaca ttaggtatat acattctagg
     3601 tattttttct cttttgttct ttattggagt tttgataatg gcaaagaagg ttagggagta
     3661 aattatggat agtataacaa tatatatgct ttttattgga aatatcgcag ttggaatttt
     3721 attttctgct catattgtat ctatgcgtat tgcagaatcg gttaatgatg gtaagataac
     3781 ttttctatca gaaatatacc aaaagattaa aaaaactgtt tccaaaataa cttatgtctc
     3841 aactgaaaaa gcaggtgagt ttgtaatgat atgctctttc tttttggtaa tatgcattct
     3901 tgtaataatc agttccggat ttttaaaagc ttttgttgtt cttatgcttt gtttgttttt
     3961 tctttatttg actaaaagaa agtgggaaaa ggatttatgt gatatttttc agaagaatgc
     4021 atataaactt tacagatatc tggtttccca gatacaagca ggtgtcagac ctaaagatgc
     4081 aatactaaat atgcataaaa taactgaaga taagcagcta aatagtattt tggcgaaagc
     4141 ttcagctgct tatgcgataa catttgatgg caaaacctat gctaatgttc ttaaagaaag
     4201 aattaaaact caggatgcgc aaaggttttg tatgatattt gaagaagatt tgttatatac
     4261 cgataatgag aattttttag agaagatgga agaaatgatg tttaacaggc atttttcata
     4321 tcatcagaaa aaaacagaga aagtaaaaag aaactgtttt gcaacggtgt gtgtatttat
     4381 gcttattgtt gtcattatgg ttgtactgcc attatttagc gagcttgtac aaggacttaa
     4441 caatatattt aattag
//
LOCUS       DAOSXM010000129         4386 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2111
            NODE_13610_length_4386_cov_11.865620, whole genome shotgun
            sequence.
ACCESSION   DAOSXM010000129 DAOSXM010000000
VERSION     DAOSXM010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864178
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4386)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4386)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:24:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,261
            CDSs (total)                      :: 2,229
            Genes (coding)                    :: 2,218
            CDSs (with protein)               :: 2,218
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 3 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4386
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13610_length_4386_cov_11.865620"
                     /isolate="AMR_MDS_2111"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            47..403
                     /locus_tag="PLQ68_10660"
     CDS             47..403
                     /locus_tag="PLQ68_10660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016322459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HXK73156.1"
                     /translation="MKEFNDNCEYIHVHEETVKVVRKNMSTEDKLYKLSDLYKVFGDK
                     TRVKILMALSSAELCVCDIATLLEMTQSAISHQLKLLRSSHLVKYRKEGKSAFYSLAD
                     DHVRTIITQGLEHIEE"
     gene            408..623
                     /locus_tag="PLQ68_10665"
     CDS             408..623
                     /locus_tag="PLQ68_10665"
                     /inference="COORDINATES: protein motif:HMM:NF012621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-associated domain-containing
                     protein"
                     /protein_id="HXK73157.1"
                     /translation="MKNVYTFEGLDCAKCADNLEIRLSKIKGVLEANVDFFMKKIYLE
                     IGDEGYEKASLIAERLIAIAVDKYIKI"
     gene            620..2470
                     /locus_tag="PLQ68_10670"
     CDS             620..2470
                     /locus_tag="PLQ68_10670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006574398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HXK73158.1"
                     /translation="MKLNRTQRVTVIKIALSVVLLLTALLIRNDTTKLIFFILSYLVI
                     GYEVLIKSAKNIIRGQVFDENFLMSIATIGAFVIREYPEAVAVMLFYEVGEFFQAYAV
                     ERSRKSITDLMKIKPDYANIERNGKLEQLSPESIKQNDVIVVKPGERVPLDGVVIEGV
                     SVLDMSSLTGESIPVDIGINDTILSGSININGLIKVRVEKAYKESTVSKILDLMENSQ
                     SKKAKTERLITRFSRYYTPIVVISALLLALIPPLLFNQSWQPWLYRALVFLVVSCPCA
                     LVLSVPLSFFSALGAASKNGMLIKGGSYLEVLAKTDTVVFDKTGTLTKGIFKVTAIHP
                     EQVSSSRLLEIATIAESYSDHPISKSLKAEYENPIDTNRIGHIEEIPGHGIKAVIDGK
                     NVAVGNDKLMEKENIEWHLCSLAGTIVHVAVDDKYMGHIVISDEIKKDAKETINQLTE
                     MGIQNISMLTGDSLASAKHIADQAGIKEYSYSLLPHQKAEYVDDLKNKMSNGKKLIFV
                     GDGINDAPVLAMADAGISMGLLGSQAAIEASDVVIMDDMPTKIPKLIRLARKTMTTAK
                     QNMVFAISIKLIVLIAGALGLASLWMAVFADVGVSVLAVLNAMRLLKKEI"
     gene            2576..3826
                     /locus_tag="PLQ68_10675"
     CDS             2576..3826
                     /locus_tag="PLQ68_10675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK73159.1"
                     /translation="MNDMTLKNVERFDMVEEIRVIKWYLRLLTWIISFPTVWLQRSKI
                     SKEGMNGIKGSYLMLCNHNAFLDFMVATAAIFPRSANYVVAIDGFIKREKLLRDVGCI
                     CKRKFTNDPILIRHLMRVAKKGQIIALYPEARYSLCGTNAILPQSLAKLVKHLHVPVA
                     TFLSKGHHINSPFWNLEKRGNRTEAHMKLIMSKEDVERMSVEEINDIINKEFVYDDFK
                     WQYENKVRVKYKNRAKGLHKVLYQCPNCKSEYKMGSEGTQIFCKNCNKRWQMTEYGRL
                     EALEGKAEFAHIPDWYEWERVQVRKQIDDGTYIFDKKVHIDSFPNAKGYIPLGEGRLI
                     HNMDGFILTGTSDEEEFRIEWPAKRLYSCHIEYEYLGKHGDCVDLNTLNDTLYIYPEG
                     DDFSVTKISLATEELYKKLNNIEG"
     gene            complement(3823..>4386)
                     /locus_tag="PLQ68_10680"
     CDS             complement(3823..>4386)
                     /locus_tag="PLQ68_10680"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HXK73160.1"
                     /translation="RGTVFFYYSDDDGYTWNRSSTMLTLPSTHTRTGLQEPGVIELPN
                     KVLYAYFRTDQMLQYESFSYDAGINWTCAQPSKFSSPDSPMKIAKNPYSNKYYAIWNP
                     IPIYNGKVKGRYVWGRTPLVIAQSDNGIDFSEPEIIEDDPLRGFCYPSVFFIDEKTML
                     LSYCSGGEKEKSCLCRISITKLTVNID"
BASE COUNT         1529 a          655 c          894 g         1308 t
ORIGIN      
        1 tatataataa catatgaaca tatattcata tgaaaggatg tgtggtatga aagaatttaa
       61 tgataattgc gaatatatac acgtacatga agaaacagtt aaggttgtca gaaagaacat
      121 gtcaactgag gataagttat ataaactgtc tgatttatat aaagtttttg gagataagac
      181 aagagttaag atactaatgg ctctttcatc agcagaatta tgcgtatgtg atatagctac
      241 attattggag atgactcagt cagccatatc acatcagttg aagcttttaa ggtcaagtca
      301 tctggtaaaa tacagaaaag aaggcaaatc agcattttat tctttagctg atgatcatgt
      361 aagaaccatt ataacccagg gcttagaaca tatagaggaa taagataatg aaaaatgtat
      421 atacatttga aggattagac tgcgctaagt gtgctgataa tctggaaatc agattaagca
      481 agataaaagg tgttttggaa gccaatgtag attttttcat gaaaaagatt taccttgaga
      541 taggtgatga aggatatgag aaagcttcat taatagcaga aagactgata gcaattgcag
      601 tggataaata tataaaaata tgaaattaaa ccgcactcag agggtaacag ttataaagat
      661 agctttatct gtcgtattgc tgttaactgc tttgttaata agaaacgata caaccaagct
      721 tatcttcttc attctctctt atcttgtaat aggatatgaa gtgcttatta aatcagcaaa
      781 aaatatcatc agaggacagg tgtttgatga gaatttttta atgagtatcg ctacaatagg
      841 agcttttgta atcagagaat atcctgaggc ggtagcagtt atgctttttt atgaagtcgg
      901 cgagttcttt caggcatatg cagtggagcg ttcaagaaaa tccattacag atttaatgaa
      961 aattaaacct gattatgcga atattgaaag aaacggaaaa ttggaacaac taagtcctga
     1021 aagcataaag caaaatgacg taattgttgt taaaccaggt gagagagtgc ctcttgatgg
     1081 tgttgtcata gaaggtgtat cagtattaga tatgtcttct cttacaggag aatcaattcc
     1141 tgttgatatc ggaataaacg atacaatatt aagcgggagc ataaatatta acgggcttat
     1201 caaagtcaga gtggaaaaag cttacaaaga gtcaacagta tcaaaaatac ttgatcttat
     1261 ggaaaattca caatcaaaaa aggcaaaaac cgaaaggctc ataacccgtt tttcaagata
     1321 ttatacgcca attgttgtaa tatcagcttt actattagct cttatcccgc ctttactttt
     1381 taaccagtcc tggcagccgt ggctgtacag agctttggtc tttttagtcg tatcatgtcc
     1441 ctgtgctttg gtgttgtcag ttccgcttag cttcttttct gcattaggag cggcatcaaa
     1501 aaacggtatg ctcataaaag gaggcagcta tttagaagta ctggcaaaaa ctgatactgt
     1561 tgtttttgac aagacaggca ctttgacaaa aggaatattc aaagttactg caattcatcc
     1621 agagcaggtg agttcatcaa ggcttcttga gattgccaca attgctgaaa gttattctga
     1681 ccatcccata tctaagtctt taaaggcaga atatgaaaat cctattgata caaacaggat
     1741 tggccatatt gaagaaattc cggggcatgg tataaaagcg gtaatagatg gaaagaatgt
     1801 agctgttggt aatgataagt taatggaaaa agaaaacata gaatggcatt tgtgctcttt
     1861 ggcaggaaca attgtacatg ttgctgttga cgataagtac atgggacata tcgtcatatc
     1921 tgatgaaata aaaaaagatg ccaaagagac aattaatcag cttacagaaa tgggaattca
     1981 aaacatatcc atgctgacag gtgacagtct tgcatctgca aagcatattg cagaccaggc
     2041 aggaataaag gaatattcat attcactttt acctcatcag aaggcggaat atgttgatga
     2101 tctgaaaaat aagatgtcaa atggcaaaaa actcatattt gtaggagatg gaatcaatga
     2161 tgctcctgtg cttgctatgg ctgatgcagg gatatccatg gggttattgg gttctcaagc
     2221 agcaattgaa gcatcagatg tagttattat ggatgatatg ccgacaaaga taccaaaact
     2281 tatacgattg gctcgaaaaa ccatgacaac agctaagcag aatatggtgt ttgcaatatc
     2341 aataaaactt atagtcctta ttgcaggagc tttaggactg gcctctttat ggatggctgt
     2401 ttttgctgat gtaggagtgt ctgtattagc agtattaaac gcgatgaggc tattaaaaaa
     2461 ggaaatctag atagtcataa aatcatcaag gacaatgttt tagttgcacc agtatttaat
     2521 aaaagatata atcaatcggt ataactcgtt tatttacatg actggggggc tttgaatgaa
     2581 tgacatgact ttaaagaacg ttgaacggtt tgacatggtt gaggaaatac gggtaattaa
     2641 atggtatctc agactcttga catggataat aagttttccg actgtatggc ttcaaagaag
     2701 taaaatttcc aaagaaggaa tgaatggaat aaaaggctct taccttatgc tttgtaatca
     2761 taatgctttt cttgatttta tggtagctac agcagcaatt tttccaagaa gcgctaatta
     2821 tgttgtagca attgatggat ttataaaaag agagaaactt cttagggatg ttggatgtat
     2881 atgtaaaaga aaattcacca atgatccgat tttaataaga catctaatga gagtggctaa
     2941 aaaaggccag attatagctt tgtaccctga agcaaggtat tctctttgtg gcactaatgc
     3001 aattttacct cagtcacttg ctaaacttgt caaacatctt catgtacctg tagcgacttt
     3061 cctttcaaaa ggacatcata taaattcgcc tttttggaat cttgaaaaaa gaggcaacag
     3121 gacggaagcc catatgaagc ttatcatgag taaagaagat gttgagagaa tgagtgtaga
     3181 ggaaataaat gacataataa ataaagaatt cgtatatgat gatttcaaat ggcaatatga
     3241 gaataaagta cgtgtgaaat acaagaacag agctaagggg ctacataaag ttctttatca
     3301 atgtccaaac tgcaaaagtg aatacaaaat gggttctgaa ggcacacaga ttttctgcaa
     3361 aaactgtaat aaaagatggc agatgactga atatggccgc ttagaagctt tagaaggaaa
     3421 agccgagttt gctcatatac ctgactggta tgaatgggaa agagtacagg taagaaaaca
     3481 gatagatgat ggtacatata tatttgataa aaaggttcac atagattctt ttccaaatgc
     3541 gaaaggatat ataccattag gtgaaggccg tttgattcat aatatggatg gatttattct
     3601 gacaggcaca tctgatgaag aagaattcag aatagaatgg cctgctaaaa ggctttattc
     3661 ttgtcatatc gaatatgagt atcttggaaa gcacggagac tgtgtggatt taaacactct
     3721 taatgatact ctttacatat atccagaagg agacgacttc tcagtaacta aaatttcttt
     3781 agctactgaa gaattgtata aaaaacttaa taatatagaa ggttaatcga tattaacagt
     3841 taattttgta atggaaatcc tgcacagaca ggatttttcc ttttcccctc cggaacaata
     3901 ggataatagc attgtctttt catcaataaa aaatactgaa ggataacaga atcctcttaa
     3961 cggatcgtct tctattattt ccggttcaga aaaatcaatt ccattatcgc tttgagctat
     4021 aactaaaggg gttcttcccc atacatatct tccctttact tttccattgt aaataggtat
     4081 tggattccaa atagcatagt atttgtttga ataaggattc ttagcaatct tcatagggga
     4141 atcaggagag gagaattttg aaggctgggc acatgtccag ttaatacctg catcatatga
     4201 gaagctttca tactggagca tctgatcagt cctgaaatat gcataaagaa ctttgtttgg
     4261 taattctata acgccaggtt cctgaagacc tgttcttgtg tgcgttgaag gaagcgtcag
     4321 cattgttgaa gatctgttcc atgtatatcc gtcatcatca gaatagtaaa aaaatactgt
     4381 tcctct
//
LOCUS       DAOSXM010000130         4300 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2111
            NODE_14002_length_4300_cov_12.128151, whole genome shotgun
            sequence.
ACCESSION   DAOSXM010000130 DAOSXM010000000
VERSION     DAOSXM010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864178
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4300)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4300)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:24:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,261
            CDSs (total)                      :: 2,229
            Genes (coding)                    :: 2,218
            CDSs (with protein)               :: 2,218
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 3 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4300
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14002_length_4300_cov_12.128151"
                     /isolate="AMR_MDS_2111"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..206)
                     /locus_tag="PLQ68_10685"
     CDS             complement(<1..206)
                     /locus_tag="PLQ68_10685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK73161.1"
                     /translation="MTNSPINFNYAFAEPHRICVCLPESSNKTLFDCTKESLMMLWSD
                     GNTKNNPFGFLSRRTFLLQEIFQG"
     gene            complement(256..1056)
                     /locus_tag="PLQ68_10690"
     CDS             complement(256..1056)
                     /locus_tag="PLQ68_10690"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HXK73162.1"
                     /translation="MIIDIHTHVFPDELAQKAKDTLLKNINYRYEPVTNLTKDSLVEK
                     MNLWGIDISVVHPVITKKTQLKNTNLWAQSISDDRIISFGGIYPHTEDYKKDIDFVCS
                     LGLTGLKFHAEYQNFEIDSTEMLRIYDYAFTKGLIIMHHTGADHGMPPPYKSNPKKIA
                     YVADQLRGGIMIAAHLGSHNMWDDVERYLMGKNVYLDTSMGFEYYSEEQFLRIVKAHG
                     ADKILFGSDSPWSNAKNEIDTISRLPIDQETKDMILGNNAKRILKINI"
     gene            complement(1196..1414)
                     /locus_tag="PLQ68_10695"
     CDS             complement(1196..1414)
                     /locus_tag="PLQ68_10695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK73163.1"
                     /translation="MKCKRCGSTDIQAIPITYGKIKRRGCLSTLFHVFMTIITLGFWI
                     IVPLIQGGSKGKVKTKIRFVCMNCGKKQ"
     gene            complement(1433..2599)
                     /locus_tag="PLQ68_10700"
     CDS             complement(1433..2599)
                     /locus_tag="PLQ68_10700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015736155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HXK73164.1"
                     /translation="MKDINVGLIGYKFMGKAHSNAFAMLPMFFDTQDRIVRKVICGRD
                     QNMVSKAAVKFGFSESMTSWEELVKRDDIHVIDITAPSNFHKEPAIEAVENKKHVFCE
                     KPLALNYKDAFMMYNAAKKAEVKNQIGFNYRFVPAIVLARKMIEQNKLGRIYHFRGAF
                     LQDWIMDPDFPKVWRLDKSVAGSGALGDLGAHVIDLARYLVADINSVCAMAETFIKKR
                     PAVQEMTGLSASKKDINAKMEKVDVDDAAIFMIKFTNGALGTIETTRFAGGHKNDMFF
                     EINGEKGSIRFYFERMNELLYYNAEDEEGYMGFRLIQASEGIHPYMQSWWPAGHVIGY
                     ENTFVHEFYEFFQSISLGHNAKPDFYDGMKCCQVLDAVELSIKENCWVSVDSIK"
     gene            2713..3741
                     /locus_tag="PLQ68_10705"
     CDS             2713..3741
                     /locus_tag="PLQ68_10705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007061783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-eliminating lyase-related protein"
                     /protein_id="HXK73165.1"
                     /translation="MINLRNDYSQVGHPRILKALSDVCLYDNEGYGLDVHSDKAALVI
                     KKLLNNESADVHFMMNGTGTNKTVLSHILRPYQAVISCNTGHINVHETGAVEATGHKV
                     IVCEGIDGKLNTKLIEKAFYSHTDEHMVMPKAVYISNATETGSIYSFNELKNIRQLCD
                     KLNLYLYMDGARLGVAMCSEDNSVKWSDLCGFFDAFCIGATKNGALLGEAVVICNEQL
                     KKDFRYSIKNGGGMYAKGFIIGMEFYELFNDGLYLDLAKNANNMAKILKEGILAAGYD
                     MEYDSSTNQLFVIVNEYQLTKLSKEITFETFYKKSENEIVIRFVTSFATTKEQIMKAL
                     KILEDLNK"
     gene            3738..>4300
                     /locus_tag="PLQ68_10710"
     CDS             3738..>4300
                     /locus_tag="PLQ68_10710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK73166.1"
                     /translation="MKTELSRLREYAKKYAEIAFSDNNLKRPDQYRKLNNLEMVRPPI
                     LVFEVPWGELEDQDELKLECETDAYRGIERGIKRNLYQFKYFEGDYAIHPYYKVKIRT
                     KSTGIGLNVEEVKINSTTGTDISAHEYHDILPDEESLERIKMPVIELDDEATKRALQA
                     AHDVFDGIMDVKLGGYQLMFNMWDELP"
BASE COUNT         1384 a          738 c          733 g         1445 t
ORIGIN      
        1 aaaccttgaa atatttcctg taataaaaaa gttcttcttg acaaaaaccc gaaaggatta
       61 ttctttgtgt ttccatcact ccatagcatc attaatgatt ctttggtaca gtcaaacaaa
      121 gttttattgc tggattcagg aaggcataca catatcctgt gtggttctgc aaatgcgtaa
      181 ttaaaattga ttggtgaatt agtcatattt cttattgtat gatacagtta ctgactgttc
      241 atatttctcc ttctttcata tatttatctt gagtattctt tttgcattat tgccaagtat
      301 catgtcttta gtttcttgat caataggaag tctgctaatg gtatctattt cattttttgc
      361 attactccat ggagaatcgg aaccgaaaag tatcttatca gctccatggg cctttacaat
      421 tcttaaaaac tgttcttctg agtaatattc aaaacccatt gaagtgtcta gatacacatt
      481 tttacccata agatatcttt ccacatcatc ccacatattg tgactgccaa gatgagcagc
      541 aatcattatt cctcctctta actgatcagc aacatatgcg atttttttag gattgctctt
      601 atacggagga ggcatgccat gatctgcacc tgtatggtgc attattatca aacctttagt
      661 gaaagcataa tcatatattc ttaacatttc agtactgtct atttcaaaat tctggtattc
      721 agcatgaaac tttaaccctg ttaatcctaa actacataca aaatcaatgt cttttttata
      781 atcctcagta tgcggatata tacctccaaa tgaaattatt ctgtcatctg agatgctttg
      841 agcccagagg ttagtgttct taagttgtgt tttcttggtt attacagggt gaacaacaga
      901 gatatcgata ccccataagt tcattttttc tactagacta tcctttgtca gatttgtgac
      961 aggttcatat ctgtagttta tattctttaa aagtgtatct tttgcttttt gtgctaactc
     1021 atctggaaaa acatgagtgt gaatatctat tatcatattc cgctcccttt tcatttattg
     1081 aatagtaaca tcttaataga ctaatttcaa gcagtggaag ggatatatgc taattaaaaa
     1141 aaagctaata ttaataataa atttgttcaa caggtactaa ctgtatatgt gtttattatt
     1201 gcttttttcc acagttcata catacaaatc tgattttagt tttcactttg ccctttgacc
     1261 cgccttgtat tagaggtaca ataatccaga accctaaagt aataatggtc ataaacacat
     1321 gaaaaagtgt agaaagacaa ccgcgtcttt ttatttttcc atatgtaata ggaatagcct
     1381 gtatatcagt acttccacat cttttacatt tcataacttc actctttatc aactatttta
     1441 ttgaatctac actaacccag cagttttcct tgattgataa ttcaactgca tcaagtacct
     1501 ggcagcattt cattccatca taaaaatcag gttttgcatt atgcccgaga gaaatgcttt
     1561 ggaagaactc atagaattca tgtacaaatg tgttttcata accaattaca tgacctgcag
     1621 gccaccatga ctgcatatac gggtgaattc cttctgaagc ctgaataagc ctgaatccca
     1681 tataaccttc ttcatcctct gcattgtagt aaagaagttc attcattctt tcaaaataga
     1741 accttatact tcctttttct ccatttattt caaagaacat atcgttttta tgaccgccgg
     1801 caaatcttgt tgtttcaata gttcctaatg ctccgtttgt gaatttaatc ataaagattg
     1861 cagcatcatc tacatcaact ttttccattt ttgcgttaat gtctttcttt gaagctgaca
     1921 gtccagtcat ttcctgaaca gctggtctct ttttaataaa tgtttctgcc attgcacata
     1981 cactgttaat atcagcaaca agatatcttg ctaaatcaat tacatgggct cctaagtcgc
     2041 ctaatgctcc tgagcccgct actgatttat ccaatctcca gacttttgga aaatcaggat
     2101 ccataatcca gtcttgcaaa aaagctcctc tgaaatgata tatccttcca agcttattct
     2161 gttcaatcat ttttcttgcc agaactatag caggtacaaa tctgtagttg aagcctatct
     2221 gattttttac ttctgctttt tttgcagcat tatacatcat gaaggcgtct ttgtaattaa
     2281 gcgctaatgg cttttcacaa aagacatgtt tcttattctc aacagcttct attgcaggtt
     2341 ctttgtgaaa gtttgaaggt gctgttatat ctataacatg aatatcatca cgttttacca
     2401 gttcttccca agaggtcata gattcactaa aaccaaattt gactgctgct ttggatacca
     2461 tattctgatc acgtccgcaa attacctttc ttactattct atcttgggta tcaaaaaaca
     2521 taggtagcat agcaaatgca tttgaatgtg cttttcccat aaatttatag cctataaggc
     2581 ctacgtttat atctttcata ctgcacgtat cctttctttg tgtattagct tctaaattat
     2641 atcatacatg actttcattg atgatgattt tattgtagaa tcaacagtat agattaatgt
     2701 atggagaaac gcatgataaa tttaaggaac gattacagcc aggtaggaca tccaaggata
     2761 ctgaaagctt tgtctgatgt ttgtctttat gataacgaag gatacggatt agatgttcat
     2821 agtgacaaag cagcattagt tattaagaag ctgcttaata acgagtctgc tgatgttcac
     2881 tttatgatga atggaacagg aaccaataaa acagttcttt cacatatatt aaggccctat
     2941 caggctgtta tttcctgtaa tacaggacac ataaatgtac acgagacagg agctgttgaa
     3001 gctacaggac ataaggtgat tgtttgtgaa ggcattgatg gaaaactcaa cactaagctt
     3061 atagagaaag ctttttacag tcatactgat gagcatatgg taatgccaaa agctgtatat
     3121 atttcaaatg caactgaaac aggaagtatc tactcattta atgagcttaa aaacattaga
     3181 caattatgtg acaaactgaa tctttatctg tatatggatg gcgcaaggct gggtgttgca
     3241 atgtgttctg aagataacag tgtcaagtgg agtgatttat gtggattctt tgatgccttt
     3301 tgtataggag caacaaaaaa cggtgcactg cttggagaag ctgttgttat ttgtaatgaa
     3361 cagttaaaga aagatttccg atatagtatt aaaaacggtg gaggcatgta tgcaaaaggt
     3421 tttataatcg gaatggaatt ttacgaactg ttcaatgatg gattatactt agatttagca
     3481 aaaaatgcga ataatatggc taaaatatta aaagaaggta tactagcagc gggatatgat
     3541 atggaatatg attcttcaac caaccagctt tttgttattg tcaatgaata tcaactgacc
     3601 aaattatcta aggaaataac ttttgagact ttttataaaa aatcagaaaa tgaaatagtt
     3661 atacggtttg taacaagttt tgcgacaacg aaagagcaaa tcatgaaagc tttgaaaatt
     3721 ttggaggatc ttaataaatg aaaacagaat tatcaaggtt aagagagtat gcaaaaaaat
     3781 atgccgagat agcatttagt gataataact taaaaagacc tgatcaatat cgaaaactga
     3841 acaatcttga aatggtaaga ccacctatac ttgtatttga ggttccctgg ggagagttgg
     3901 aagaccagga tgaacttaaa cttgaatgtg aaactgatgc ttacagagga attgaaagag
     3961 gcattaaaag aaatctttat cagtttaagt attttgaagg agattatgcg atacatccgt
     4021 attacaaggt taaaataagg acaaaaagta caggaatagg actcaatgtt gaagaagtta
     4081 aaataaattc cacaacaggt accgatataa gtgcacacga atatcatgac attctgccag
     4141 atgaagaatc acttgagagg atcaagatgc ctgttattga gctggatgat gaagcaacaa
     4201 aaagagcttt acaggcagca catgatgttt ttgacggaat aatggatgtc aaacttgggg
     4261 gataccagct tatgtttaat atgtgggatg agcttccaag
//
LOCUS       DAOSXM010000131         4195 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2111
            NODE_14525_length_4195_cov_9.412077, whole genome shotgun sequence.
ACCESSION   DAOSXM010000131 DAOSXM010000000
VERSION     DAOSXM010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864178
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4195)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4195)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:24:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,261
            CDSs (total)                      :: 2,229
            Genes (coding)                    :: 2,218
            CDSs (with protein)               :: 2,218
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 3 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4195
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14525_length_4195_cov_9.412077"
                     /isolate="AMR_MDS_2111"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(108..190)
                     /locus_tag="PLQ68_10715"
     tRNA            complement(108..190)
                     /locus_tag="PLQ68_10715"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(154..156),aa:Leu,seq:gag)
     gene            382..1227
                     /locus_tag="PLQ68_10720"
     CDS             382..1227
                     /locus_tag="PLQ68_10720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK73167.1"
                     /translation="MSNNKKLSNNEQKPIETKFSKFMDILKVRFWDVAKYNLIALGLF
                     FIFILFYAIFVSQIFFPIDVSDYQQGYSNFGELWAVLVYRIPYVLILFTIPVVAFGPV
                     ASAMAVVFRSYVRRDDSFSSDFTDGMKKFFWKSLAVSTISTVITVCLGIAFRFYQMNL
                     SAGFWKTALLLVIAGFSLLFLMMHMYIYQILVEYNLSIWKLYRYAYVFSILRFFPNLL
                     IGISIIALTMLPFMLHILFGAVVMFTVLFGLTSYIINFFAWPALEKHLDPIMKEQKVK
                     KVKLY"
     gene            complement(1306..2061)
                     /gene="dapB"
                     /locus_tag="PLQ68_10725"
     CDS             complement(1306..2061)
                     /gene="dapB"
                     /locus_tag="PLQ68_10725"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006355224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HXK73168.1"
                     /translation="MMNILLVGCNGKMGTTIARICSERNDISISCGLDVTKKDNMPFP
                     VYTNEKQIPVSINIDAVIDFSHPSGVIQTANFCKRSKSPLVYATTGLDKESNKLLDEL
                     SDFVPVFRSANMSLGVNLLIKLCKNAASFLKEDFDIEIVEKHHNQKIDAPSGTALKIA
                     DEINSTLDNEMNYVYDRHNTRNKRSKKEIGMHSIRGGNIVGEHDVIFAGLDETITISH
                     GAYSKDVFAQGALRAALYIKDKPAGMYSMDDML"
     gene            complement(2058..2927)
                     /gene="dapA"
                     /locus_tag="PLQ68_10730"
     CDS             complement(2058..2927)
                     /gene="dapA"
                     /locus_tag="PLQ68_10730"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010252124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HXK73169.1"
                     /translation="MLFKGAATAVITPFNKEGIDFENFDKQIDIQLNCGIQAIIVCGT
                     TGEASTMTEQERYEAIAFTVKKVNKKIPVIAGTGCNDTHHSIRLSKDAYTAGADAILV
                     TAPYYNKTSKKGLIEHFRTISESVPIPTILYNIPSRAGMNIDPDVIKHLAENCNVIAI
                     KECNLSQMADVIQSTPDNFTVYSGDDCINLPALSLGAMGVVSVLSNILPSHVAMMNKL
                     FLENKVDQSRELFYKSRKLVNLLFIETSPIPVKKAMHLMGLDSGLLRLPLVDMDEKNA
                     QLLKDEMINQGVI"
     gene            complement(2929..3927)
                     /locus_tag="PLQ68_10735"
     CDS             complement(2929..3927)
                     /locus_tag="PLQ68_10735"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HXK73170.1"
                     /translation="MKKYNFAIVGATGMVGRTFIKVLEERNLPIENIYFFASARSAGS
                     TITFLGKDYMVEELNEHSFDRKTDIALFSAGGDLSKIYAPIASKHGAVVIDNSSAWRM
                     DPSVPLVIPEVNKEDIEKHKGIIANPNCSTIQALVAVKPLHDAFKLKRMIVTTFQSVS
                     GAGKSGCDELENSLKGLPPKKFPHPIANNCIPHIDVFLDNGYTKEEMKMVNETRKMFH
                     DDSLKITCTCVRVPVMKSHSESINVEFDNQYELDDIYNILNSAPGVKVVDDVKNSKYP
                     LATDAADTDDTYVGRIRRDESVENGLNIWVVADNVRKGAASNAVQIAEELIKVWER"
     regulatory      complement(3997..4174)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
BASE COUNT         1207 a          767 c          694 g         1527 t
ORIGIN      
        1 gtagacgaat atcaaagcct tataacaatg aagcactaga cgtttgtatt aagagaatct
       61 ggtatattaa gattaataaa aacggaatca aatgattccg ttttctttgg tgcggtcatc
      121 gggacttgaa cccgaatggt ctcccatacg cccctcaaac gtacgcgtct gccagtttcg
      181 ccatgaccgc tcacttaaga aattatatct atcagcatag tgtttgtcaa cggtaaaagt
      241 tactaatttc aaagtcgttt tatctttatc tttgttgaat gaaaatcaat tgttaatcaa
      301 ggttaccata tatatactct acctctcttg ataaaaagtt gaaaagtgta taaaatatat
      361 caatgcaaaa ggagctgact aatgagtaat aacaaaaagt taagcaataa tgaacaaaaa
      421 ccgattgaaa caaaattttc gaaatttatg gatatactaa aagtcaggtt ttgggatgta
      481 gcaaaatata acctgatagc attaggcttg ttttttattt tcatactatt ttatgcaata
      541 tttgtatctc agatattttt cccaatagat gtttctgatt atcagcaggg atattcgaat
      601 tttggagagc tttgggcggt acttgtttac aggatacctt atgttctaat cttatttacc
      661 atacctgttg tagctttcgg accagtagca tcagctatgg cagttgtttt tagaagctat
      721 gtcagaaggg atgacagctt ttcatcagat tttacagatg gaatgaagaa attcttttgg
      781 aaatcgttag cggtatcaac tatatctaca gtaataactg tttgtttagg catagctttc
      841 agattttacc agatgaattt atcagctggt ttttggaaaa ctgctttact gcttgttatt
      901 gcaggatttt cactgttgtt ccttatgatg catatgtaca tatatcagat tcttgttgag
      961 tacaatcttt ccatatggaa gctgtacagg tatgcttatg tgttttcaat attgagattc
     1021 tttccaaatt tattaatagg gatttctata atagcattaa caatgcttcc atttatgctt
     1081 catattctat ttggagcagt tgttatgttt acagtacttt ttggattaac ttcatatatt
     1141 ataaatttct ttgcatggcc tgcactggaa aaacatcttg atccaattat gaaagagcag
     1201 aaagtgaaga aggttaaatt gtattaaaga ttttcaatga attgtaatag ttaatagcaa
     1261 taaaaaaagc agttaaactg ctttttttta gtcagtaaat tatgtttaca gcatatcatc
     1321 catgctgtac attccagcag gtttatcttt tatataaagt gctgctctta acgctccttg
     1381 cgcaaatacg tctttggaat atgcaccatg tgatatggtt atagtttcgt caagtccagc
     1441 aaaaatcaca tcgtgttccc caactatatt accgcctctg attgagtgca ttccaatctc
     1501 tttcttgctt cttttatttc ttgtattgtg acggtcatat acatagttca tttcattgtc
     1561 taaagtgctg ttaatttcat cagctatctt taaagcagtc ccgctaggtg cgtctatttt
     1621 ctggttgtga tgtttttcaa ctatctcaat atcgaaatct tcttttaaaa aacttgcagc
     1681 atttttacat agctttataa gaagattgac acccagactc atattagcgc ttctgaatac
     1741 tggtacgaaa tctgacaact catcaagaag tttattggac tctttatcca atcctgtagt
     1801 tgcgtacact aaagggcttt tgcttctttt gcagaaattt gcagtttgta ttactcctga
     1861 tggatgtgaa aagtctataa cagcatcaat gtttatagaa actgggattt gcttttcgtt
     1921 tgtgtaaaca gggaagggca tattgtcttt ttttgttaca tcaagaccgc agcttatcga
     1981 tatgtcgttt ctttcagaac atattcttgc aatagttgtt cccatttttc cattgcatcc
     2041 tacaagcaga atattcatta tataactcct tgattaatca tttcatcttt tagcagttgc
     2101 gcattctttt catccatatc tactaaaggc aaacgaagca ggccggaatc caaacccatt
     2161 agatgcattg ctttttttac aggtatcggg cttgtctcta taaatagcag attaacaagt
     2221 tttctgcttt tgtaaaacaa ctctctgctt tgatctactt tgttttctaa aaacagttta
     2281 ttcatcatgg ctacatgaga aggcagtata ttggaaagga cagatacaac tcccattgct
     2341 cctaaagata gtgcaggaag attgatgcag tcatccccgc tgtataccgt aaaattatcc
     2401 ggagttgatt gaataacatc agccatttgt gataaattgc attcctttat tgctattaca
     2461 ttacaattct ctgcaaggtg tttaataaca tcaggatcaa tattcatacc tgctcgtgag
     2521 ggaatgttat acaatattgt tggtatgggt acactttctg atatggttct gaaatgctct
     2581 ataagacctt tttttgaggt tttgttgtaa tatggagcag tgaccagtat tgcgtcagct
     2641 ccggctgtgt atgcgtcttt tgataatctg atgctatgat gtgtatcatt acatcctgta
     2701 cctgcaataa ctggtatttt tttattcact ttctttactg tgaaagctat agcttcgtat
     2761 ctttcctgtt ctgtcatagt tgaagcttca cctgttgttc cgcacactat aattgcttgt
     2821 ataccacaat tcagctgaat atcgatttgt ttatcaaaat tttcgaaatc aataccttct
     2881 ttgttaaatg gtgttataac ggctgtcgcc gcacctttaa ataacatatt atctctccca
     2941 tacttttata agttcttcag caatctgaac tgcattgctt gcagctcctt ttcttacatt
     3001 atctgcaact acccagatgt taaggccatt ttctacagat tcatctcttc tgattctgcc
     3061 gacatatgta tcatctgtat ctgcagcatc agtagctaat ggatattttg aatttttaac
     3121 atcatcaacc acttttactc ctggtgcact attcagtata ttatatatat catcaagctc
     3181 atactgatta tcaaactcaa cattaataga ttcgctatga cttttcataa ctggaaccct
     3241 tacacatgta caggtaattt tcaaactgtc atcatgaaac atttttcttg tttcatttac
     3301 cattttcatt tcctcttttg tatatccgtt atctaagaaa acatcaatat gaggtataca
     3361 gttattagct atcgggtgag ggaatttctt tggtggaaga ccttttaacg agttttcaag
     3421 ttcatcacat ccgctttttc ctgcacctga aactgattga aatgttgtaa caatcattct
     3481 ttttaattta aatgcatcat gtaatggttt aacagcaact aaagcctgaa ttgtagagca
     3541 gttaggatta gctataattc ctttatgttt ttctatatct tctttattta cttcaggaat
     3601 aactaaagga acagatggat ccattctcca tgcgctgctg ttatcaataa ccactgcacc
     3661 atgcttactt gcaataggtg catatatctt acttaaatca ccgcctgcgg aaaataaggc
     3721 aatatctgtt tttctgtcaa aggaatgttc gttaagttct tcgaccatat aatctttgcc
     3781 taaaaaggtg attgtgctgc ctgctgatct tgcagaggca aaaaagtaga tgttttcgat
     3841 tggaaggttt ctttcttcaa ggacttttat aaaggttctt cctaccatac ctgtagcgcc
     3901 tactattgcg aaattgtact ttttcatttt tcaccaacct gtttgtcata ttatatcaaa
     3961 aaaaggctgc atgtgcgagc ctttacttaa tgattcttaa gtgacagctc tccaccgtta
     4021 gttttgcggt gacagtcaat tcgctatttg caaatcaacc agataaacgc atataaggct
     4081 acgttttctt cggcgataat cccttttacc atctgtcaat cttccttatg tcgtccaaat
     4141 gattactatt gagtaccgcg cctctatcaa tattagtgga agtataacaa gcata
//
LOCUS       DAOSXM010000132         4165 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2111
            NODE_14687_length_4165_cov_10.237226, whole genome shotgun
            sequence.
ACCESSION   DAOSXM010000132 DAOSXM010000000
VERSION     DAOSXM010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864178
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4165)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4165)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:24:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,261
            CDSs (total)                      :: 2,229
            Genes (coding)                    :: 2,218
            CDSs (with protein)               :: 2,218
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 3 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4165
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14687_length_4165_cov_10.237226"
                     /isolate="AMR_MDS_2111"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            74..949
                     /locus_tag="PLQ68_10740"
     CDS             74..949
                     /locus_tag="PLQ68_10740"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HXK73171.1"
                     /translation="MAYSMTGYGKSLYENELISMRIEIKSVNSKFLDLSLRLPKNISY
                     AEEKVRNLIRSRISRGKVDCYVTVYNISADFLDIKFDEKAADSYYTFLKTIQERYNLN
                     NDITPVQIASFQGVISVQESDEDNEKLTESILEAFNIAIDNILDTKQKEGNALSKDCI
                     EKIKSFSEYVDNIEKLSFDIPTQYKQKLEKRLEELLSDVKIDDAKITQEVAFFADRAC
                     VDEEITRLKSHINQFISNIDKDSGGKKLDFIIQEMLRETNTIASKANNLDITNNTLEM
                     KYLLEKIREQIQNLE"
     gene            952..1224
                     /locus_tag="PLQ68_10745"
     CDS             952..1224
                     /locus_tag="PLQ68_10745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004617885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF370 domain-containing protein"
                     /protein_id="HXK73172.1"
                     /translation="MKFLNIGFGNMVSMERIVAVLSPESAPVKRIIQEARDTSLLIDA
                     TYGRRTRSVIITDSEHIILSPLQPETLERRLSGKIVPDDINEDNND"
     gene            1259..1504
                     /locus_tag="PLQ68_10750"
     CDS             1259..1504
                     /locus_tag="PLQ68_10750"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF013365.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HXK73173.1"
                     /translation="MINKPSVEELEKKIPVRFIIVNAIAKRSREIASGAKIRINTSEN
                     NPISVAAMEINSGEVRVTDEPSPLNIQTEQTVESEIA"
     gene            1504..2895
                     /locus_tag="PLQ68_10755"
     CDS             1504..2895
                     /locus_tag="PLQ68_10755"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015557354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HXK73174.1"
                     /translation="MDRSQITMEKLVSLAKRRGFVYPGSEIYDGLANAWDYGPLGVEL
                     KSNIKNAWWQKFIKENEYNVGIDSAILMNPNIWVASGHVGSFVDPLTDCKNCKTRHRA
                     DDLIKEYNNTNNIDISVEGFDNTALMEYIKKNKIPCPNCGKHDFTDVKQFNCMFKTFQ
                     GVNEDTAAQIYLRPETAQGIFVNFKNVQRTTRKKIPFGIGQIGKSFRNEITPGNFIFR
                     TREFEQMELEFFCEPGTDLEWFEYWRTFCYNWLISLGINKENIKLRDHTEKELSFYSN
                     ATTDFEYKFAFGWGELWGIADRTDYDLKQHSKFSNDDLTYFDPMTNEKYVPYCIEPSL
                     GVERLLLALLTDAYDEEELEDGDIRTVLRFHPYIAPIKAAVLPLSKKLSDEALKVFNM
                     LRKKWNVEYDDTQSIGKRYRRQDEIGTPFCFTYDFDSINDECVTVRYRDSMKQDRIRI
                     DAIMTFLDENIKL"
     gene            2907..>4165
                     /locus_tag="PLQ68_10760"
     CDS             2907..>4165
                     /locus_tag="PLQ68_10760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HXK73175.1"
                     /translation="MTKYVYLFKEGNAKMKNLLGGKGANLAEMTSLGLPVPRGFTVTT
                     EACLRYYEDGEQISDDIKEEILKTLEITEKTVGKKLGDPLNPFLVSVRSGARASMPGM
                     MDTVLNLGLNDVVVEGLAELTSNTRFAYDSYRRFVMMFSDVVIGLSKAKFERILDEIK
                     EEKGVTNDTDLSAEDMKFVLEKFKDMYKKEIGKDFPQDPKEQLFEAIKAVFRSWNNER
                     AIYYRRMNDIPSSWGTAVNVQEMVYGNMGDDSGTGVAFTRNPSTGEKKLMGEYLMNAQ
                     GEDVVAGVRTPYHIDHLEVTNKAVYDQFMELSSKLENHYHDMQDIEFTIEKGKLFILQ
                     TRNGKRTAHAALKIAVDLVREGLLTKEEAIVKVDPKQLDALLHPMFETKALKSAEVIG
                     TGLAASPGAATGKVVFSAEDAKEMHKKG"
BASE COUNT         1596 a          534 c          834 g         1201 t
ORIGIN      
        1 gcagttaaaa tttaattgca tattatggtt atttatgtta caatacttca gattaatgta
       61 ggaagtataa ccaatggcat atagcatgac aggttatggt aaaagcctgt atgaaaatga
      121 actaatatca atgaggattg aaatcaagag cgtaaacagt aaatttcttg atttatcttt
      181 aagattgcca aaaaatatat cgtatgctga agaaaaagtt cgcaacctta tacgctcaag
      241 aatttcaaga ggtaaagttg attgttatgt aactgtgtat aatataagtg ctgatttttt
      301 agatattaaa tttgacgaaa aagcagcaga ctcatattac acatttctta aaacaattca
      361 ggaaaggtat aatctcaata atgatatcac tccagttcaa atagcatctt ttcaaggagt
      421 aataagtgta caggaaagcg atgaagataa tgaaaaactg actgaaagca ttttggaagc
      481 atttaatatt gctattgata atattctcga tacaaaacaa aaagaaggta acgccttatc
      541 aaaagactgt attgaaaaaa tcaagagttt tagtgaatat gtagacaata ttgaaaaatt
      601 atcattcgat attcctactc agtataaaca gaaacttgaa aaaagacttg aggagctatt
      661 aagtgatgtc aagatagatg atgcaaaaat aacccaagaa gtagcttttt ttgcagacag
      721 ggcatgtgtt gatgaggaaa ttacaagact aaaaagtcat ataaatcagt ttattagcaa
      781 tattgataag gattcaggtg gtaagaaatt agattttatt atccaagaga tgttaaggga
      841 aaccaataca atagcatcaa aagcaaataa tcttgatatt acaaataaca cactggaaat
      901 gaaatattta ttggaaaaga taagggagca gattcaaaat ctggagtaat tatgaaattt
      961 ttaaatatcg gattcgggaa tatggtttca atggagagga ttgtagcggt tttaagtcca
     1021 gagtctgctc cagttaaaag aataatccag gaagcaagag acacatcatt acttatagat
     1081 gctacatatg gcagaaggac aagatcagtc attataacag acagtgagca tataatattg
     1141 tcgcctttac aaccagagac actagaaaga cgtttaagtg gaaaaatagt accagatgat
     1201 ataaatgaag ataataacga ttagctaata tcataaaata aggagaatat acaaaaatat
     1261 gataaacaaa ccgtcagttg aggaattaga aaagaaaata cctgttagat ttattatagt
     1321 gaatgctata gcaaaaaggt ctagagaaat tgcatcaggt gcaaagatta ggattaatac
     1381 ttccgaaaat aatcctattt ctgttgcagc aatggaaatt aacagtggtg aagtaagggt
     1441 tacagatgaa ccttcgccac taaatattca aacggagcaa actgttgaaa gtgagattgc
     1501 ttaatggata gaagccaaat tactatggaa aaattagtct ccttggcaaa gaggagaggt
     1561 tttgtttatc ctggatcaga aatctatgat ggtttagcaa atgcatggga ttatggtcct
     1621 ttaggagtag agttaaagag taacattaag aatgcatggt ggcagaaatt tattaaagaa
     1681 aatgaatata atgtaggtat agatagtgcc attcttatga accctaatat atgggtagca
     1741 tcaggacatg tagggtcttt tgttgatcct cttactgact gtaaaaattg caaaacaaga
     1801 catcgtgctg atgatcttat taaagaatac aataatacta acaatataga tatttctgtc
     1861 gaaggttttg ataatactgc tttgatggag tatatcaaaa aaaataaaat accatgtcca
     1921 aattgcggca aacatgattt tacggatgtt aagcaattta actgtatgtt taaaacattt
     1981 caaggagtta atgaagatac tgctgcacag atttacttaa gaccagaaac agcgcaagga
     2041 atattcgtta atttcaagaa tgttcagaga acgacacgta aaaaaatacc atttggtata
     2101 ggacagattg gaaagagttt tagaaacgaa ataacaccag gtaattttat attcagaaca
     2161 agagaatttg aacagatgga actggaattt ttctgtgaac ctggtactga tttagaatgg
     2221 tttgaatatt ggaggacttt ctgctacaac tggcttatca gtcttggtat aaataaggaa
     2281 aatatcaaat taagagatca taccgaaaaa gaattatcat tttatagtaa cgcgacaact
     2341 gattttgaat ataaatttgc atttggctgg ggtgagcttt ggggaattgc agataggaca
     2401 gattacgatt tgaaacagca ctcgaaattt tcaaatgatg atctcacata ttttgatcct
     2461 atgacaaatg aaaagtatgt tccatactgt atagaacctt ccttaggggt tgaaaggcta
     2521 ctgttagcat tattgacaga tgcatatgat gaagaggagt tggaagatgg cgatataaga
     2581 acagttttaa gatttcatcc ttatattgca ccaataaaag cagctgttct tcctttatca
     2641 aaaaaactaa gcgatgaagc attgaaggtt ttcaatatgc ttaggaaaaa atggaatgtt
     2701 gaatatgacg atacacaaag cataggaaaa agatacagaa gacaagatga aataggaaca
     2761 cctttctgtt ttacatatga ttttgattct ataaatgatg aatgtgttac ggttaggtac
     2821 agagacagca tgaaacaaga taggattcgt attgatgcta ttatgacctt tcttgatgaa
     2881 aatattaaat tataacggag ggaaatatga caaaatacgt ttatctcttt aaagagggga
     2941 atgcgaaaat gaaaaatctt cttggtggaa aaggagctaa ccttgctgaa atgacttcac
     3001 taggattacc tgttccaagg ggatttactg tgacgactga agcctgttta agatattatg
     3061 aagatggaga acagatatca gacgacataa aagaagaaat attaaaaaca ctagaaataa
     3121 ccgaaaaaac ggtgggaaaa aaactaggag atcctctgaa tccattttta gtttcagtaa
     3181 gatcaggagc tagagcttca atgcctggaa tgatggatac tgtattaaac ttaggtttaa
     3241 atgatgtagt agtagaagga ttagcagaac ttacgagtaa tacaagattt gcttatgata
     3301 gctacagaag atttgttatg atgttttctg atgtcgtaat aggtttatca aaagcaaaat
     3361 ttgaaagaat tctagatgaa ataaaagaag aaaaaggcgt tactaatgat actgatcttt
     3421 cagcagagga tatgaagttt gttctggaaa aatttaagga catgtataaa aaggaaatag
     3481 ggaaagattt ccctcaagat cctaaagagc agctctttga agcaatcaaa gctgttttca
     3541 gatcatggaa taacgaaaga gctatttatt acagaagaat gaatgacata ccttcaagtt
     3601 ggggaacagc tgttaatgtt caggagatgg tatatggtaa tatgggcgat gattcaggaa
     3661 ctggtgttgc ttttacaaga aatccatcaa caggcgagaa gaagcttatg ggtgaatatc
     3721 taatgaatgc ccaaggagag gatgttgttg ctggagttag aacaccatat cacattgatc
     3781 atttagaagt tactaacaaa gctgtttatg atcagttcat ggaactttct tcaaaacttg
     3841 agaatcatta tcatgatatg caggatatag aatttactat tgaaaaaggt aaattattta
     3901 tacttcaaac cagaaatggt aaaagaactg cacatgcagc tcttaaaatc gcagttgatc
     3961 ttgtaagaga aggtctttta acaaaagaag aagcaattgt aaaggttgat ccaaaacagc
     4021 ttgatgctct tttacatcca atgtttgaaa ctaaagcatt aaaatctgca gaagttatag
     4081 gcactggttt agcagcttca cctggtgcag caactggaaa agtagttttt tctgctgaag
     4141 atgctaaaga gatgcacaaa aaagg
//
LOCUS       DAOSXM010000133         4107 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2111
            NODE_15018_length_4107_cov_9.538746, whole genome shotgun sequence.
ACCESSION   DAOSXM010000133 DAOSXM010000000
VERSION     DAOSXM010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864178
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4107)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4107)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:24:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,261
            CDSs (total)                      :: 2,229
            Genes (coding)                    :: 2,218
            CDSs (with protein)               :: 2,218
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 3 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4107
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15018_length_4107_cov_9.538746"
                     /isolate="AMR_MDS_2111"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..225
                     /locus_tag="PLQ68_10765"
     CDS             <1..225
                     /locus_tag="PLQ68_10765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HXK73176.1"
                     /translation="NPPYHADFSVPKEFIEKGYRKLVPNGRMLMVTKRLEWYKRKFIS
                     VFGGVKIFRCDDYFVFMGIKKSKNKENMHI"
     gene            325..1374
                     /locus_tag="PLQ68_10770"
     CDS             325..1374
                     /locus_tag="PLQ68_10770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013297179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HXK73177.1"
                     /translation="MEYKLGIIGYGGMASWHHRNSVKVPGLKVTSAFDIDPERLKVAA
                     ESGLKIYDDINDFLTNGDFSIVLVATPNNFHKYYSVMAMEAKKHVVCEKPVAMNLEEL
                     DEMIEASKKNNVLFTVHQNRRWDRDYRIVKQILSENTIGKPYTIESRVHGQNGVMHGW
                     RAYKVAGGGMLFDWGVHLIDQMLYMVDEKVTEVYCQLFSIKTPEVDDYFKLILRFESG
                     LSAQIEVGTYCLEKMPRWYVNADQGSVIVKNWECEGKITKASQIVMEWEPEVVQTVGG
                     PTRTMAPRPKETLEELELPKIQSEWAEYYKNVISYLNKKDDIIVKQGELRRVMKVIEA
                     AFESDKLHQSIKCNI"
     gene            1385..1717
                     /locus_tag="PLQ68_10775"
     CDS             1385..1717
                     /locus_tag="PLQ68_10775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013700910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="HXK73178.1"
                     /translation="MNQKADMYFMTNQKYFPTDKLYAVKERLDTMTDDQIQRLMYLQL
                     KDPITYLLVSIFIGELGIDRFLLGDIGMGILKLLTAGLCGILWIYDMVTIQDKVKNMN
                     FEELMRIM"
     gene            1779..1997
                     /locus_tag="PLQ68_10780"
     CDS             1779..1997
                     /locus_tag="PLQ68_10780"
                     /inference="COORDINATES: protein motif:HMM:NF018644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HXK73179.1"
                     /translation="MLMCYDKKRAINGEFRISESVFFILSLLGGALGTMFGMRYCRHK
                     TKKPAFKILVPVFLLLNLSVLFLIYFYT"
     gene            complement(1998..4043)
                     /locus_tag="PLQ68_10785"
     CDS             complement(1998..4043)
                     /locus_tag="PLQ68_10785"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HXK73180.1"
                     /translation="MDYLNYVNIKQGTRSNPRFSNGNTLPLTQLPFAMASFTVQTDGS
                     RESWFYHPDDKSIEGVRLTHQPSPWIGDYGALLMLVQSGICGTDYRSIWSGFDQTKAT
                     LTPNFLSVRFIKSNASLSLAPTQTGASMQLIFDDYRTPYFTLMSLGGKSKFSYDKDTG
                     KLTGYTENIHRMPKAGFRMYFIYQFDTDDVDVSDILISNTPKSLENGVSVEGDRISIH
                     IAFKNNIVNLSMCTSYISYKQAELNHSREIKNLSLKEVSDSAAFIWQEHLSRIEVKTN
                     SIKQMKTFYSCLYRCFLFPHKCYELDINEKPIHFSPYDGKIHDGVRYTDSGFWDTFRT
                     LFPLFSIIAKEEYKSMLEGFVNDYIESGWLPRWPSMVEVGCMPSTLIDAVIADAAVKN
                     IADKPLLEKALEGMIKHANVKSDGKKHGRNGVEDYIKLGYVPYEDESESVNLTLDAAY
                     GDFCISVVADKLGKDELKEKYLNRSKNYIHLFDKSTGFMRAKDRNGQFRPDFNKFMWG
                     RDYTEGCAWQNSFSVYHDLENFAKLYGEDADFIRMITEIFETPPLYYTDNYGREIHEM
                     TEMASADFGQCAISNQPSFHIPYLFSYFGMQHRTDYWVERICNEVFSEDESGFPGDED
                     NGATSSWYIFSSLGLYPICPGKAEYIKGPMLIKSAKILGNKWSNKGFTKIIPHNVLG"
BASE COUNT         1363 a          647 c          761 g         1336 t
ORIGIN      
        1 aatccaccat atcatgcaga tttctcagtg cctaaggagt ttattgaaaa aggatataga
       61 aaacttgtac caaatggcag aatgctaatg gttacaaaaa ggcttgaatg gtataaaaga
      121 aaattcatat ctgtcttcgg tggcgttaaa atattccgtt gtgatgatta tttcgttttt
      181 atggggataa aaaaaagtaa aaataaagag aatatgcata tataattatt tatatactat
      241 tcattacatc ttaataaaat attaaattaa ttgatgttga cattacttct gcttagtaat
      301 aatatggtgc agatggaggt tgttatggaa tataaattgg gaataatagg gtatggcgga
      361 atggcatcat ggcatcatag aaattctgtc aaagtaccag gtttaaaagt tacgagtgca
      421 tttgatatag accctgaaag acttaaggtt gccgcagaaa gcggtttgaa aatatacgat
      481 gatattaatg attttttaac aaatggagat ttttccatag tattggtagc tactccgaat
      541 aattttcaca aatactattc agtaatggca atggaagcca aaaagcatgt agtctgtgaa
      601 aaaccggttg cgatgaatct tgaagaactt gatgaaatga ttgaggcatc aaaaaagaat
      661 aacgttcttt ttactgtcca tcaaaacaga agatgggata gagattacag gatagtgaaa
      721 cagattcttt cagaaaatac aattggaaaa ccatacacta tagaatcgag agttcatggc
      781 cagaatggtg taatgcatgg atggagagca tataaagttg ctggcggagg aatgctgttt
      841 gactggggag ttcatcttat agatcagatg ctatatatgg ttgatgaaaa agttactgaa
      901 gtatactgcc agttgttcag tataaaaaca ccggaagtag atgactattt caaattaata
      961 ttaaggtttg agtcagggtt atcagctcag attgaagttg gcacatactg tttggaaaaa
     1021 atgccaagat ggtatgtaaa tgcagaccag ggttcagtaa tagtgaaaaa ctgggagtgc
     1081 gaaggtaaga tcactaaagc cagccagata gtaatggaat gggaaccaga ggtagtacag
     1141 acagtaggag gaccaacacg tactatggct cctcgtccaa aagagacttt agaagaactg
     1201 gaactaccga aaattcaaag cgaatgggca gaatactata aaaatgttat ttcgtatttg
     1261 aacaaaaaag atgatataat tgtaaagcaa ggtgaattaa gaagagtcat gaaagttatt
     1321 gaagcagctt tcgagtcgga taaattacac caaagcatta aatgcaatat ataaaggggc
     1381 aattatgaac caaaaagcag atatgtattt tatgactaat caaaagtatt ttccaactga
     1441 caagttatat gcagttaagg aaagacttga tacaatgaca gatgatcaga tccaaaggct
     1501 tatgtattta cagcttaaag accctatcac ttatctatta gtttccattt ttattggtga
     1561 acttggtatt gacaggtttc ttcttggcga tatcggaatg ggtattttaa agcttttgac
     1621 agcaggtctg tgcggtatac tgtggatata tgacatggtc actattcagg ataaggtcaa
     1681 aaacatgaat tttgaagaac ttatgaggat tatgtaatat cagtgtgttt tttaagataa
     1741 tccttgtatc agatatagta ctttgtttat cgtgctatgt gttaatgtgt tatgacaaaa
     1801 aaagagccat aaacggtgag tttagaatta gtgaatctgt ttttttcatt ttatcgttac
     1861 taggtggcgc tttaggcact atgtttggga tgcgttactg cagacataaa acaaaaaaac
     1921 cggcatttaa aatacttgta ccggtttttc ttttacttaa tttatcagtt cttttcctaa
     1981 tatattttta tacctgatta acccaaaaca ttatgaggta taatttttgt gaatccttta
     2041 ttgctccatt tgtttcctag tatctttgca ctttttataa gcatggggcc tttaatgtat
     2101 tctgcttttc ctgggcatat tggataaagg ccaagtgagc tgaatatata ccatgatgat
     2161 gtagctccat tatcctcatc tcctggaaaa cctgactcgt cttctgaaaa aacttcatta
     2221 caaattcttt caacccagta atcagttctg tgctgcattc cgaaatatga gaataaatac
     2281 ggtatgtgga aagaaggttg attagatatg gcgcattgac cgaaatctgc tgatgccatt
     2341 tctgtcatct catgaatttc tcttccataa ttgtctgtgt aataaagagg aggagtttca
     2401 aatatttctg ttatcattct aataaaatct gcatcttctc catatagttt agcaaaattt
     2461 tcaagatcat gataaacaga aaaggaattc tgccatgcgc aaccttcagt gtagtcccgt
     2521 ccccacatga atttattgaa atcaggacgg aattgtccat ttctatcttt tgctctcata
     2581 aatcctgtgg atttatcgaa gagatgaatg tagttttttg acctatttaa atatttttct
     2641 tttaactcat cttttccaag tttatctgct acaacggaaa tacagaaatc gccatatgca
     2701 gcatcaagag taagatttac gctttcagat tcatcttcat atggtacata gccaagcttt
     2761 atataatctt caacaccatt tcttccatgc tttttgccat cagatttaac attagcatgt
     2821 ttaatcatac cttcaagagc tttttcaagt aaaggtttat cagcaatatt tttaacagca
     2881 gcatctgcaa taacagcatc aataagtgtt gatggcatac atccaacttc gaccattgat
     2941 ggccatcttg gcaaccagcc tgattcgatg taatcattta caaatccttc aagcatactt
     3001 ttatattctt ctttagctat aatagagaaa agagggaata aggttctaaa ggtatcccag
     3061 aaaccactgt cagtatacct tacaccatca tgaatttttc catcatatgg actgaaatga
     3121 attggttttt cgtttatgtc cagctcgtag catttatgag gaaacagaaa acatctgtac
     3181 aaacatgaat aaaaggtttt catttgcttt atgctgtttg ttttaacttc aatacgtgaa
     3241 agatgctctt gccatatgaa agcagcacta tcgcttactt cttttaaaga caaatttttg
     3301 atttcccttg aatggttaag ttcagcctgt ttataagata tatatgaagt acacattgat
     3361 aggtttacta tgttattttt aaaagcaata tgtatggata ttctgtcgcc ttcaacagaa
     3421 actccatttt ctaaagattt tggagtattt gatattaata tatctgatac atcaacatca
     3481 tctgtatcga attgataaat aaagtacatt cgaaaacctg ctttgggcat tctgtgaata
     3541 ttttcagtat aacctgttaa cttacctgta tctttatcat aactgaattt actttttccc
     3601 cctaatgaca ttaatgtgaa atatggggtt ctgtaatcat cgaaaatcaa ttgcatagat
     3661 gcacctgttt gtgttggagc aagggaaagc gatgcattgg atttaataaa tctcacagag
     3721 aggaaattag gagttaaagt tgcctttgtc tggtcaaatc ccgaccatat gcttctgtaa
     3781 tcggtgccac agataccgct ttgaacaagc ataagtaacg caccgtaatc tcctatccaa
     3841 ggactaggct ggtgtgtaag acgaactcct tcaatactct tatcatcagg atgataaaac
     3901 catgattccc ttgaaccatc tgtctgtact gtaaaacttg ccatagcaaa gggtagttgc
     3961 gtaagaggga gagtgtttcc attggagaat ctgggattgg atcttgtacc ctgtttaata
     4021 ttaacataat ttaaatagtc cataataata cctcgcaagt actattatat tccaaatcaa
     4081 agtttatgta aaaaattaaa atgatat
//
LOCUS       DAOSXM010000134         4098 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2111
            NODE_15062_length_4098_cov_9.591887, whole genome shotgun sequence.
ACCESSION   DAOSXM010000134 DAOSXM010000000
VERSION     DAOSXM010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864178
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4098)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4098)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:24:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,261
            CDSs (total)                      :: 2,229
            Genes (coding)                    :: 2,218
            CDSs (with protein)               :: 2,218
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 3 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4098
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15062_length_4098_cov_9.591887"
                     /isolate="AMR_MDS_2111"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..417
                     /gene="udk"
                     /locus_tag="PLQ68_10790"
     CDS             <1..417
                     /gene="udk"
                     /locus_tag="PLQ68_10790"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HXK73181.1"
                     /translation="HLEKLKNNESIECPIYSFTEHTRTKQTRTIKPTKVIIVEGILIF
                     QNPKLLNLMNIRIYVDTDPDVRILRRILRDVKERGRSLDSVVTQYLSTVKPMHEQYVE
                     PSKRNADIIVLEGGYNLVALDMIIQRIQGHVDDKEV"
     gene            419..1780
                     /gene="uxaC"
                     /locus_tag="PLQ68_10795"
     CDS             419..1780
                     /gene="uxaC"
                     /locus_tag="PLQ68_10795"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF014652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HXK73182.1"
                     /translation="MKKKYIYSQSGQILYDQIAKELPIIDYHCHLSPKQIYEDMPFDN
                     IGSMWLGADHYKWRLMRAYGIDEEYITGNTSWKDKFFAYCKTIENCPNSPLYHWSHME
                     LSMYFDTEMNISYDNAQMIWDKANEVISKKQLSPRKLISQSKVIYIATTDDITDDLEY
                     HKLLQQDSEFMTRVMPTFRTDNVLLINRPDYINYIDKLSELTAVMINNIDKLCEAIAK
                     RLDYFVTNGCKISDVGIQYTPNPVFTKESAQNAFMQALKGKNADKSDYEQFLGYMFVF
                     LSGEYKKRNITQQIHTGVYRNANTRLYNSIGADCGCDMSNNPLDAVLFANLLDQMDKN
                     NNLAKTIVYILNPSSYYQALTACGCFRDVIFGAAWWHMDHYEGIAANLRTVSSLSCLG
                     SSMGMLTDSRSFLSYARHDYYRRILCSVVGSWVDADEYPIELAKNLIYNLCVGNASKR
                     FLE"
     gene            1803..2513
                     /locus_tag="PLQ68_10800"
     CDS             1803..2513
                     /locus_tag="PLQ68_10800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK73183.1"
                     /translation="MKKTIWIIVIVIIILSIGAVSGAMAGIAVNKFVDEYNLEVVYPD
                     VDEATYSMLDKIGIGSEQLSGFSTLQEALLNIEGEVEISNIGFTSEEITAILNVSPEY
                     LKSMFVKSVVFDIQSDDTVNLEVYLDAEEINRNSDIPDIAIGIIQSTKFTINISITGV
                     SEKGELIVVVNDIYVKDVHIMNFMNIFPVGSEFKGYVSEIMDKEIKVLLPMLKDLKSF
                     EISDGYAYIDAIFTYGGN"
     gene            2515..3570
                     /gene="mnmA"
                     /locus_tag="PLQ68_10805"
     CDS             2515..3570
                     /gene="mnmA"
                     /locus_tag="PLQ68_10805"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HXK73184.1"
                     /translation="MHENKKKSVLIGISGGVDSAVGAYILKNQGYDVHAVTYILSSSQ
                     KSSVNDAIKIAEKLNIPLYIDDKTDSFDKEVISYFTNAYLKGETPNPCVLCNKSFKFR
                     HLIERAESMGIDYVATGHYANIDYDDKSKRFLLTKSANEKKDQSYFLYRLTQSQLSKI
                     IFPISDMNKDQVRLIAQKIGLEVNDKKDSQDICFIKDTDYASFIKEKTDRLFENSTFI
                     DKSGNTIGKGDNHIFYTPGQRRGLGQGFNKRVYVIKKDAMNNKVVLGDETDLYKKEIK
                     VKDINLISIVKLEEPMKVKIKIRNSMNFADGEISMVDDNIKIVFDEPVRAAVNGQSAV
                     FYKDNLVVGGGIIYDAL"
     gene            3557..>4098
                     /locus_tag="PLQ68_10810"
     CDS             3557..>4098
                     /locus_tag="PLQ68_10810"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HXK73185.1"
                     /translation="MTLYKGILDMIGNTPLIELCAIKQKYMLHGDIYAKVESFNPGGS
                     VKDRTAFSLIQSLKNNDRYNKAVRENAKITIVEATSGNTGIALAMICARLGYELIICM
                     PENMSKERVMILKAYGAKVILTDGTRGMQGAIEKSLEFKDAFTLCQFENPYNPLIHEN
                     TTAKQIYDDLKGDIDIFVAGV"
BASE COUNT         1488 a          540 c          807 g         1263 t
ORIGIN      
        1 catcttgaga aattaaaaaa taatgaaagc atagaatgtc caatatattc atttacagaa
       61 catacaagaa ctaaacagac cagaaccata aaacctacta aagtaataat tgttgaggga
      121 attttgatat ttcaaaatcc gaaattacta aatttgatga atataaggat atatgtagat
      181 acagaccctg atgtaagaat actaagaaga atactaaggg atgtaaagga aagaggcaga
      241 agtttagatt cagtagttac tcaatattta tctactgtta aacctatgca tgagcagtat
      301 gttgaaccat ctaaaagaaa tgcagatata attgtacttg aaggcggata taatcttgtc
      361 gctttagaca tgataattca aagaattcaa ggacatgtag atgataagga agtgtaatat
      421 gaagaaaaag tatatttatt cgcaatcagg acagatttta tatgaccaga tagcaaaaga
      481 actgccgatt atagattatc attgccatct gagcccaaaa cagatttacg aggatatgcc
      541 ctttgataat ataggtagca tgtggcttgg ggcagaccac tacaaatgga ggctcatgcg
      601 ggcttatggt atagatgaag aatatattac tggaaatacg agttggaagg ataaattctt
      661 tgcatactgt aaaactatag agaattgtcc caatagccct ctttatcatt ggtcacacat
      721 ggagctttct atgtattttg atactgaaat gaacatttca tatgataatg cacaaatgat
      781 atgggataaa gcaaatgaag ttatatctaa aaagcaactg agtcctagaa aacttatatc
      841 acagtcaaaa gtcatataca tagcaacaac agatgatatt acggatgatc ttgagtatca
      901 taaactgtta caacaagaca gtgaatttat gacaagagta atgcctacat tcagaacaga
      961 caatgtatta ttgataaaca gaccagatta tattaattac attgataaac taagtgaatt
     1021 aactgctgtt atgataaata atatagataa gctttgtgaa gctatagcaa aaagactgga
     1081 ctactttgtt accaatggat gtaagatatc cgatgtgggt atccaataca cgcctaatcc
     1141 ggttttcaca aaggaatcgg ctcaaaatgc ttttatgcaa gcccttaaag gtaagaatgc
     1201 agataaatct gattatgagc agttcttagg atatatgttt gtctttcttt ctggtgaata
     1261 caagaaaaga aatattactc agcagattca tacaggtgta taccgtaatg ctaataccag
     1321 actatataac agcattggag ctgactgtgg atgtgatatg tcaaataatc cgcttgatgc
     1381 tgttttgttt gctaatcttt tagatcagat ggataagaat aacaatcttg caaagacaat
     1441 agtatatatt cttaatcctt catcatatta tcaggcattg actgcctgcg gctgttttag
     1501 agatgtaata ttcggtgctg cgtggtggca tatggatcat tatgagggta tagctgctaa
     1561 tttaaggacc gtttcatcat tgtcatgtct tggttccagt atgggtatgc ttactgacag
     1621 cagaagcttc ttgtcatatg cgcgtcatga ttattacagg cgtatattat gctctgttgt
     1681 gggttcttgg gttgatgctg atgaataccc gattgaatta gcaaaaaacc tgatatataa
     1741 tctgtgcgta ggaaatgctt ccaaaagatt tttagaatga ccatttcctc tggaggtgct
     1801 taatgaaaaa aactatctgg attattgtta tagttataat tatattatct attggagcgg
     1861 tatcgggtgc aatggctggt attgctgtaa acaagtttgt tgatgaatac aatctggagg
     1921 ttgtttatcc ggatgttgat gaagcaacat acagtatgct tgataaaata gggattggca
     1981 gtgaacaatt aagcggtttt tcaactttgc aggaagcctt acttaatatt gaaggtgaag
     2041 tcgaaataag caatattgga tttacatctg aagaaataac agctatatta aatgtttcac
     2101 cggaatattt aaaatccatg tttgtaaaat ctgttgtttt tgatatacag tctgatgata
     2161 ctgtaaattt ggaagtttat ctggatgctg aagaaataaa caggaattca gatattcccg
     2221 atattgctat tggtattata caaagcacaa aatttacgat aaatataagt attacaggag
     2281 tatcggaaaa aggcgagctt attgttgttg taaatgatat atatgtaaaa gatgtgcata
     2341 ttatgaattt tatgaatatt tttcctgtag gttcagagtt taaaggttat gtcagcgaaa
     2401 taatggataa agaaattaag gttttattgc ctatgcttaa agatctgaaa tcttttgaga
     2461 tatctgacgg atatgcatat attgacgcaa ttttcacata tggcggtaat tgatttgcat
     2521 gaaaataaaa aaaaatcagt actcataggc atttcaggtg gagtagatag cgcagtagga
     2581 gcatatattc ttaaaaatca aggatatgat gtgcatgcag ttacatatat actttcttct
     2641 tctcaaaagt catctgttaa tgatgcaata aagatagctg aaaaattaaa tattccttta
     2701 tatattgacg ataagactga tagctttgat aaagaggtaa tatcatattt cacgaatgca
     2761 tatttaaaag gtgagactcc taacccatgc gtattatgta ataaatcatt taagttcaga
     2821 catctaatag aaagagctga aagtatgggg attgattatg tagcgacagg tcattatgct
     2881 aatatagatt acgatgacaa atcaaaaaga tttttactta ctaaatctgc aaatgaaaaa
     2941 aaagaccagt cgtactttct atacagattg acacagagtc agttaagcaa aattattttt
     3001 ccaatttctg atatgaataa agatcaggtt agattaatag cgcaaaagat aggacttgaa
     3061 gtaaatgaca agaaagacag ccaggatata tgttttataa aagatactga ttatgccagt
     3121 tttatcaaag aaaaaacgga caggttattt gaaaacagca cttttattga taaatcaggt
     3181 aatactattg gtaaagggga taatcacatt ttctatactc cgggtcagag aagaggactt
     3241 ggacaaggtt ttaacaaaag ggtatatgtt attaaaaaag atgcaatgaa caataaagtt
     3301 gtattaggtg atgaaactga tctttataaa aaagaaataa aggttaaaga tattaacctt
     3361 atatccatag taaaactcga agaacctatg aaagttaaaa taaagataag aaattccatg
     3421 aattttgctg atggtgagat atctatggta gatgataata ttaagattgt atttgatgaa
     3481 cctgtaagag ccgctgtaaa cgggcagtca gcagtttttt acaaagataa tcttgtagtt
     3541 ggaggaggaa ttatatatga cgctctataa aggaattctt gatatgatag gaaatacacc
     3601 attaatcgaa ctttgtgcta taaagcagaa atatatgtta catggagata tatatgcgaa
     3661 agtggagagt tttaatccag gaggttcagt caaggacaga acagcattct ctttgattca
     3721 gagcttaaaa aataatgaca gatataataa ggctgtcagg gaaaatgcaa aaataaccat
     3781 tgtagaggct actagcggca acacaggcat agcattggca atgatttgtg caagactggg
     3841 ttatgaatta attatctgta tgcctgagaa catgtctaaa gaaagagtta tgattttaaa
     3901 agcatatgga gctaaggtta ttcttactga tggcacaaga ggaatgcaag gtgctattga
     3961 gaaatcctta gagtttaaag atgcgttcac tctttgtcag tttgaaaatc catacaatcc
     4021 tcttatacat gaaaatacta ctgcaaagca gatatatgat gatttgaaag gggatataga
     4081 tatatttgtg gctggagt
//
LOCUS       DAOSXM010000135         4066 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2111
            NODE_15239_length_4066_cov_9.433308, whole genome shotgun sequence.
ACCESSION   DAOSXM010000135 DAOSXM010000000
VERSION     DAOSXM010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864178
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4066)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4066)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:24:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,261
            CDSs (total)                      :: 2,229
            Genes (coding)                    :: 2,218
            CDSs (with protein)               :: 2,218
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 3 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4066
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15239_length_4066_cov_9.433308"
                     /isolate="AMR_MDS_2111"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            250..1494
                     /locus_tag="PLQ68_10815"
     CDS             250..1494
                     /locus_tag="PLQ68_10815"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004631124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004121 - cystathionine beta-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HXK73186.1"
                     /translation="MKTYNFDRIIDRRNTNSLKYDYAKDFGYTKDHIPLWVADMDFSV
                     IEEIKDAIYKINAVGVYGYTGISENYYKIVRDWMLKRYNFKVDKQWHCITPGVVYAMS
                     TAINAFTQPNESVIIQTPVYSPFYRVVTNNKRNLIKNSLKYENETYSVDYADFEQKIV
                     DNNVKLFLLCNPHNPVGKVFTKEELIRICHICCKHKVIIVSDEIHQDFVYEPNKHTVL
                     ANLNANFEQCCVVCTSPSKSFNIAGLKVSNVFIPNPELKKIYDEELIRRGPSSVNTLG
                     IAACMAAYKYGSEWLDQLKDYLKGNIDFVEEFISQHMPRIKVLKPQGTYLMWLDCSNM
                     GLNNEQVNSFFKDEAKLWLNPGDVFGPEGQCFQRINIATSRSILQKAMDRIKTAYDVL
                     YHDKNEFDLLKAESEILDIANE"
     gene            1531..2808
                     /gene="serS"
                     /locus_tag="PLQ68_10820"
     CDS             1531..2808
                     /gene="serS"
                     /locus_tag="PLQ68_10820"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010963346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HXK73187.1"
                     /translation="MLDINRIRNNKEEVEKALLKKNFSVDFTELLEWDSLRRKSIFDV
                     EQMKAKKNTVSADIPKMKKQGLDVTELLVEMKKISTSIVEYEKIINELDNSIKQFLEV
                     IPNTPANDVIAGDESNNTPVRYYKEKPKFGFVPKDHVTICENLGLIDYKTGAKLSGNG
                     YWLYRNEGALLEWALLNYFISEHIKDGYEFILPPHILNYECGYTAGQFPKFTDDVYKI
                     EQSNQFLLPTAETALINVHRNEVLKEEELPKKYFAYTPCYRKEAGSYRAEERGMIRGH
                     QFNKVEMFQYTTPEQSEDALIEMVEKAENLMKGLQLHFRVSKLAAADCSFAMGKTFDI
                     EVWIPSMGIYKEVSSASNSYDFQAIRGSMRFKRTQTGKNEYLHTLNASGLATSRVFPA
                     IIEQHQNKDGSVNVPDVLKKWINKDKITVNGIL"
     gene            2795..4039
                     /gene="lysA"
                     /locus_tag="PLQ68_10825"
     CDS             2795..4039
                     /gene="lysA"
                     /locus_tag="PLQ68_10825"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020815676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HXK73188.1"
                     /translation="MEYFSENKSFYKTHTPSELLKQYGSPLYVYNEDILREQAKKIKT
                     LVSLPNFEVSYSIKANSNLHILKILKEEKLHADAMSDGEIYILEKAGYKSDHIFYVCN
                     NVSVEEMRYAVDRNIMISIDSLSQLELYGKNFKDTKVSIRLNPGIGAGHHQKVVTGGK
                     TTKFGINLNKIQDVHKISEKYNLTIVGINQHIGSLFMSSTEYIRGVKELLQTALEFKD
                     LEFIDFGGGFGIQYRKQEGQEPLELEKFGLELTEVLKNWQNKYKKNILFRCEPGRFVV
                     AESAILLGTVHAKKSNYEKIFIGTDIGFSVLIRPAMYGSFHDIEIYRKDKLVKEKPFE
                     TVSITGNICESGDILVEERELPRIQEGDLIGVMDAGAYGYSMSSNYNNRLRPAEILIK
                     SDNSIQLIRKRDSYEDLLKGFD"
BASE COUNT         1567 a          500 c          745 g         1254 t
ORIGIN      
        1 cgcctctttt taattatatc aaatatatgt atctgattca aacttcttta tcagtgtaaa
       61 ttaatattat acaaagatta attaaaattg attactagcc gtaaaaaaat gtgaaaatgg
      121 tgttaatata aaaagcagaa ttatttatat atttaaaaaa ggtaaattga ctgttgaaat
      181 atcagtattt atatagattt aaaattctta agtgttataa tattttgaag aaggaaggta
      241 gagaaaaaat tgaaaacata taattttgat aggataattg atagaagaaa taccaattca
      301 ttaaaatatg attatgcaaa agattttggt tatacaaaag accatatacc tctttgggtt
      361 gcagatatgg atttttcagt aattgaagaa attaaagatg caatatacaa aataaatgct
      421 gttggtgtat atggttatac aggaatatca gaaaattatt ataaaatcgt tagagattgg
      481 atgttaaaaa ggtataattt caaagtagac aaacaatggc attgtattac accaggtgtc
      541 gtatatgcaa tgagcactgc tattaatgcc tttactcaac ctaatgaaag tgtaattatt
      601 caaactccgg tgtattcacc tttttacaga gtagtaacta ataataaaag aaatcttatt
      661 aaaaactctt taaaatatga gaatgaaact tatagtgttg attatgctga cttcgaacaa
      721 aaaatagtgg ataataatgt aaaacttttt ctattgtgta atccacataa tcctgttgga
      781 aaagtcttta ctaaagaaga acttataagg atttgtcaca tctgctgtaa acacaaagtt
      841 ataatagtca gtgatgaaat acatcaggat ttcgtatatg aaccaaataa gcatactgtt
      901 ttagctaatt taaatgctaa ttttgaacaa tgttgtgtag tatgtacttc acctagtaaa
      961 tcgttcaata ttgcaggttt gaaagtatct aatgtattta ttccgaatcc tgaacttaag
     1021 aaaatttatg atgaagaact tataagaaga ggaccaagca gtgttaacac attaggcatt
     1081 gctgcatgta tggctgcata taagtatgga agtgaatggc ttgatcagtt aaaagattat
     1141 ttaaaaggaa atattgattt tgtagaagaa tttatttcac agcatatgcc cagaataaaa
     1201 gttctcaaac cacaaggaac atatcttatg tggcttgatt gttcaaatat gggtttaaat
     1261 aatgaacaag ttaattcatt ttttaaggat gaagcaaaat tatggcttaa cccaggtgat
     1321 gtattcggac cggaaggaca atgttttcag cggattaaca tcgctacaag caggtcaata
     1381 ctgcaaaaag caatggatag gattaaaaca gcttatgatg ttctatatca cgataaaaac
     1441 gaatttgatt tattaaaagc tgaaagtgaa attcttgaca ttgcaaacga atgattttat
     1501 tattaactaa aatattaagg ggatgattaa atgctggata tcaatcgtat aaggaataat
     1561 aaagaagaag tagaaaaagc tcttttaaag aagaattttt ctgtagactt taccgagctt
     1621 cttgaatggg attcgcttag aagaaaaagc atatttgatg ttgagcagat gaaagcaaag
     1681 aagaatactg tatctgctga tattccaaaa atgaaaaaac agggattgga tgttactgaa
     1741 cttcttgttg aaatgaaaaa aatatcaaca agtattgtag agtatgaaaa aataataaat
     1801 gaacttgata atagtattaa gcagtttctt gaggttattc caaatactcc tgcaaatgat
     1861 gttattgcag gggatgaatc aaataatact ccagtaagat attataagga aaaacctaaa
     1921 tttggtttcg ttccaaaaga tcatgtaacg atatgtgaaa acctggggtt gattgattat
     1981 aaaacaggtg ccaaattaag cggtaacggg tactggcttt acagaaatga aggcgctttg
     2041 cttgaatggg cacttttaaa ctattttatt tcagaacata taaaggatgg ttatgaattt
     2101 attcttccgc cacatatttt aaattatgaa tgcggttata ctgcaggtca gttcccgaaa
     2161 tttactgatg atgtatacaa aattgaacaa agtaatcaat ttttattacc aacagctgaa
     2221 acagcactta taaatgttca caggaatgag gttttaaaag aagaagaact tccaaagaag
     2281 tattttgcat atacaccttg ttacagaaaa gaagcgggca gttacagagc tgaagaaaga
     2341 ggtatgataa gaggacatca atttaataag gttgaaatgt ttcaatatac tacccctgaa
     2401 caaagcgaag atgctttgat agagatggtt gaaaaagctg agaatttgat gaaaggtctg
     2461 caacttcatt tcagggttag caaacttgct gctgccgatt gttcatttgc aatgggaaag
     2521 acatttgata ttgaagtttg gataccaagt atggggattt ataaagaggt aagttctgct
     2581 tcaaattctt atgattttca agctattaga ggctcaatga gatttaaaag aacacaaacc
     2641 ggcaaaaacg aatatcttca tacattgaat gcatcaggtt tggctacaag cagagttttt
     2701 ccagcaataa tagaacagca tcagaataaa gacggttcag ttaacgtacc agatgtacta
     2761 aaaaaatgga ttaataagga taaaataaca gttaatggaa tactttagtg aaaataaaag
     2821 tttttataaa acacacaccc cttcagagct cttaaagcaa tatggatctc cattgtatgt
     2881 atacaatgag gatattttac gtgaacaggc aaaaaaaata aaaaccttag tcagtttacc
     2941 taattttgaa gtatcgtatt caattaaagc caattcaaat cttcatatat tgaaaatttt
     3001 aaaggaagag aaacttcatg ccgatgcaat gagcgatgga gaaatatata tacttgaaaa
     3061 agcgggttac aaaagtgatc atatttttta tgtttgcaac aatgtttctg ttgaagaaat
     3121 gagatatgca gtagatagaa acataatgat aagtattgat tcgttatcac agcttgagtt
     3181 gtatggtaag aattttaaag atacaaaagt atcaataaga ttgaatccag gtataggagc
     3241 tggccatcat cagaaagtgg ttacaggtgg taagacgact aagtttggaa taaatcttaa
     3301 taaaatacag gatgtacata aaatatcaga aaaatataac cttacaatag taggcataaa
     3361 tcagcacata ggatccttat tcatgtcttc aacggaatat ataagaggag ttaaggaatt
     3421 actacaaaca gcacttgaat tcaaggacct cgaatttatt gattttggag gaggttttgg
     3481 tattcagtat agaaagcaag aaggtcagga accattggaa cttgagaagt ttggtcttga
     3541 attgaccgaa gtattaaaaa attggcaaaa taaatacaaa aagaatattt tatttagatg
     3601 tgaacctggt agatttgtag tagctgaatc tgcaatactt cttgggacag ttcacgcaaa
     3661 gaaaagtaac tatgaaaaaa tattcatagg cacagatatc ggtttttctg ttcttatacg
     3721 acctgcaatg tatggttcat ttcacgatat tgagatatat agaaaagaca agctagttaa
     3781 agaaaaacct ttcgaaacag taagtattac aggcaatatt tgtgaaagtg gcgatatact
     3841 tgtagaagaa cgagaacttc caagaattca agaaggagat ttaataggag ttatggatgc
     3901 tggagcatat ggatattcta tgagttctaa ttataataac agattgcgac cagctgaaat
     3961 attaataaaa tctgataaca gtattcaact tataagaaaa cgtgattcct atgaagattt
     4021 gttaaaaggt ttcgattaac tttttaacta tatcagtaat gattta
//
LOCUS       DAOSXM010000136         3873 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2111
            NODE_16397_length_3873_cov_9.536668, whole genome shotgun sequence.
ACCESSION   DAOSXM010000136 DAOSXM010000000
VERSION     DAOSXM010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864178
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3873)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3873)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:24:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,261
            CDSs (total)                      :: 2,229
            Genes (coding)                    :: 2,218
            CDSs (with protein)               :: 2,218
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 3 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3873
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16397_length_3873_cov_9.536668"
                     /isolate="AMR_MDS_2111"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..830)
                     /locus_tag="PLQ68_10830"
     CDS             complement(<1..830)
                     /locus_tag="PLQ68_10830"
                     /inference="COORDINATES: protein motif:HMM:NF015662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK73189.1"
                     /translation="MGIIARILKNRYFIIALVLSLIMVLFLGKLYKLQIIDGDLYISQ
                     SAVRIERRYSISSARGNIYDRNGVLLAYNQECQNVYLTKAFTASDDLNPTLLCLYDLL
                     TQNNEQFSNSISSVLEYNQEFETFVFNKNWTEEDLLLWQTRTDLFALSESKVYYDAYD
                     FFKYLRKYFFIDDSYSDLDAYKIITFRYEILKMRPEYLEGYIEIATDVSFETVAFIAE
                     NSHKLKGVSLRKSYVRAYTPQAALISHIIGYVGLVSDTELENEPELSVEYMIGKMGIE
                     L"
     gene            complement(805..1338)
                     /gene="mreD"
                     /locus_tag="PLQ68_10835"
     CDS             complement(805..1338)
                     /gene="mreD"
                     /locus_tag="PLQ68_10835"
                     /inference="COORDINATES: protein motif:HMM:NF016019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HXK73190.1"
                     /translation="MYNYKWYAYILIAVNLILEPYISKWFSIGIAYPDVLLILILMLS
                     MGKKDSQIIIISSVTGFIYDMLYSSFLGPMTILLFLRGLVSIIIGRRFKKENIFVLTT
                     VGFIMAFISRLYTIILSSGISDFIRGFSLFIQDIGFSALYTSLIIMLITIILFIVYNS
                     FGKRSKRHFYGHNSQDT"
     gene            complement(1339..2172)
                     /gene="mreC"
                     /locus_tag="PLQ68_10840"
     CDS             complement(1339..2172)
                     /gene="mreC"
                     /locus_tag="PLQ68_10840"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HXK73191.1"
                     /translation="MKLLKNRKFQIIIIIVVFMVVAILSINRFSGVNIIRNLVTIPVS
                     WIQKGINNIDEYFTSKSFTKKQYEELLEENKNLTEENEKLQEQNAELSFLMDENILLR
                     EALYLKDIFDGYDIIGSNIMGSDPGNFVYRYKIDIGSRDNVKIDMPVVAANNTLYGRI
                     YMVNYTTSIIIPIIDENAGISGWISKTDGGHVNIKGDIRYREKGQCLIENISDKVNLE
                     VGDIVETSGFGGIYPKGILIGVITEVFYDDITNEQFGVIEPYINFNSINTVFVLMEKT
                     E"
     gene            complement(2183..3193)
                     /locus_tag="PLQ68_10845"
     CDS             complement(2183..3193)
                     /locus_tag="PLQ68_10845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015084796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HXK73192.1"
                     /translation="MSLFGRDIGIDLGTANTLIYERNKGIILREPSVVAMESKSRKVV
                     AVGNDARAMLGRTPQNIITVKPLKDGVIADYQGTLGMLKEFIRKAIGRAIISKPSMII
                     CVPAGVTKVERKAVIEAGLEAGAKEIFDPEESLMAAVGAGLPVFEPTGNMIIDIGGGT
                     TEVAVISMGNIVNSKTLRSAGDTFDELITQYIKKKYRVIIGARTAEDLKINIGNVMPF
                     DEPKTLVIRGRNADQGLPVNLTVSSEEISEAMHPVILEILEAIRATLELTPPELAADV
                     MANGIVLSGGGALISGLDRYLSEKTGNIPVRVADKPLECVAIGAGKMLDKLDYFKGIT
                     KV"
     gene            complement(3223..>3873)
                     /gene="radC"
                     /locus_tag="PLQ68_10850"
     CDS             complement(3223..>3873)
                     /gene="radC"
                     /locus_tag="PLQ68_10850"
                     /inference="COORDINATES: protein motif:HMM:TIGR00608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HXK73193.1"
                     /translation="RPYEKLYEKGAASLSDTELLAIVIKSGSSGKNAMDLSRLLLSDD
                     ELGKGLNGLSQAGIQQIMKHKGIGKVKAIQIKACLELANRLKPKHEKKVKLTTPDQIA
                     YHMMPKAQGLNKEKCWVLCFDVRMNLIKEVTISIGTLDSAIVHPRDIFTEVIKSCAHS
                     FIVIHNHPSGDVNPSSNDITLTKRLKKAGLLLGMELRDHIIISEENYFSFLSKSML"
BASE COUNT         1220 a          758 c          497 g         1398 t
ORIGIN      
        1 agctcaatac ccatttttcc tatcatgtat tcaacgctta attccggttc gttttccagt
       61 tctgtatcag aaactagtcc aacatatcct ataatatgtg aaatcaatgc tgcctgtgga
      121 gtatatgctc tgacataact tttacgcaaa gatactcctt tgagtttatg actgttttct
      181 gctatgaatg caactgtttc aaaagacaca tctgttgcaa tctcgatata cccttcaaga
      241 tactctggac gcattttcaa tatttcatat ctgaatgtaa tgattttata tgcatccaaa
      301 tcggagtagc tgtcgtctat aaagaaatat tttcttaaat atttaaaaaa atcatatgca
      361 tcataatata ccttcgattc agataatgcg aataaatcgg ttctagtctg ccacaataat
      421 agatcttctt ctgtccagtt tttattaaaa acaaaagttt caaattcctg gttatattct
      481 agcaccgaag aaatactatt actaaactgc tcattgtttt gtgtaagtaa atcataaaga
      541 caaagtaatg taggattaag gtcgtcactt gcagtaaaag cttttgtaag atagacattc
      601 tgacactctt gattatatgc taataaaact ccgtttctat cgtatatatt cccccttgct
      661 gaactgatgc tatatcttct ttcaattcta actgctgatt gacttatata caaatcacca
      721 tcaataatct gaagtttata cagtttgcca agaaacagta ccataattag ggacaatact
      781 aaagctatta taaaataccg gtttttaagt atcctggcta ttatgcccat aaaaatgtct
      841 tttgctcctt ttgccaaatg aattgtatac tatgaaaaga ataatagtta ttagcattat
      901 tatcaaagag gtatataaag cagaaaaacc aatatcctga ataaataaac taaatcctct
      961 tataaagtct gatatgcctg atgataaaat aattgtatat aaccttgata taaatgccat
     1021 aataaaacca accgtagtca gaacaaatat attctctttt ttaaatcttc gtccaataat
     1081 aatagataca agtcctctta agaatagcaa gatagtcata ggacctaaaa atgaactgta
     1141 aagcatatca taaatgaaac ctgttactga agaaatgata attatctgac tatccttttt
     1201 ccccattgaa agcataagaa taagaatcaa gagaacatca gggtatgcaa tacctattga
     1261 gaaccatttt gatatatatg gttctaatat gagattgaca gctattagga tatatgcata
     1321 ccatttatag ttatacattt attctgtttt ctccattaac acaaaaactg tgtttattga
     1381 gttaaagtta atatatggtt caataacacc aaactgctcg ttggtaatat catcataaaa
     1441 gacttcagta attactccta ttaatatgcc ttttggatat ataccgccaa aaccgcttgt
     1501 ttcaacaata tctcctactt caagattaac tttgtctgaa atattttcaa ttaaacactg
     1561 ccccttttcc ctgtatcgta tatcaccttt tatattaaca tgacctccat cagtttttga
     1621 aatccatccg cttataccag cattttcatc aattatcggt ataataattg atgtagtgta
     1681 gttaaccata tatattcttc cgtacaaagt attatttgct gctacaacag gcatgtctat
     1741 ttttacatta tctcttgaac ctatatctat tttatatcta tatacaaaat taccgggatc
     1801 agaacccata atattacttc caataatatc gtaaccatca aaaatatctt ttaaatacaa
     1861 agcctctcta agaagtatgt tttcatccat caaaaaagat aattcagcat tttgttcttg
     1921 aagcttttca ttttcttctg taaggttttt attttcctct agtagctctt catattgttt
     1981 ttttgtaaaa cttttcgaag tgaaatattc atcaatatta tttatacctt tttgaatcca
     2041 tgatactggt attgtcacca gatttctaat aatattaaca ccgctaaatc tattaataga
     2101 taagatagct acaaccataa ataccacaat aattataatt atttggaatt ttctgttttt
     2161 aagaagtttc aattttcact ccttatactt ttgttatacc tttgaaatag tctaatttat
     2221 caagcatttt tccagcccca attgcaacgc attctaaagg tttatctgca actctcacag
     2281 ggatattgcc tgttttttca cttaaatacc tatctagtcc cgatattaaa gcaccacctc
     2341 cacttaaaac aattccattt gccattacat cagcagccaa ctcaggtgga gtaagttcaa
     2401 gtgtcgctct tattgcttca agaatttcga gtattaccgg atgcatagcc tcacttattt
     2461 cttcactact gacagtcaga ttaactggta atccctgatc tgcattacgt ccacggataa
     2521 ccaatgtctt aggttcgtca aaaggcatta cattcccaat atttatttta agatcctctg
     2581 ctgtcctcgc gcctataatt actctgtatt ttttcttgat atactgggtt attaattcat
     2641 caaaggtatc tcctgctgac cttaaagtct tgctgtttac tatattaccc atagaaataa
     2701 cagctacttc agtagttcct cctccgatat caattatcat attgcctgtt ggctcaaaaa
     2761 caggaagtcc tgctcctact gcagccatta aactttcctc tggatcaaaa atctctttcg
     2821 caccagcttc aagaccagct tctattacag cttttctttc aactttagtt actccggccg
     2881 gaacacatat aatcattgaa ggcttggaaa taatcgctct tccaatagcc tttctgatga
     2941 attccttcaa catgcctaat gtaccctgat aatcagcaat tactccatct tttagaggtt
     3001 ttacagtaat tatattctga ggagttcttc caagcatagc tcttgcatca ttacctactg
     3061 caacaacttt tctgcttttt gattccattg ctaccactga gggttctctt aatattattc
     3121 ctttatttct ttcatatatt aatgtattgg ctgtacctaa atcaatccca atatctctgc
     3181 cgaataatga catattatct ctttcctttc ttatctagtt tattatagca tgcttttact
     3241 aagaaaacta aaataatttt cctctgaaat gatgatatga tctcttaatt ccatacctag
     3301 taataatccc gcttttttta atctttttgt caatgttata tcattagaac tgggatttac
     3361 atcacctgaa ggatggttat gtataactat gaaactatgt gcgcatgact ttattacttc
     3421 tgtaaagata tctcttggat gaactatagc actatctaaa gtacctatgg aaattgtaac
     3481 ttcttttatg agattcatcc ttacatcaaa gcataaaacc cagcatttct ccttattcag
     3541 cccttgtgct tttggcatca tatgatatgc tatttgatca ggagtcgtta attttacttt
     3601 tttttcatgt tttggcttta atctgtttgc tagttcaaga caagctttaa tctgaattgc
     3661 ttttacctta ccaatacctt tgtgtttcat tatctgctga ataccagctt gagaaagacc
     3721 attcaaacct tttcccagtt catcatctga aagtagaagt cttgacaaat ccatagcatt
     3781 ttttccactg gaaccagatt taattacaat cgctaacaat tcggtatcag aaagagatgc
     3841 agcacccttt tcatatagtt tttcataagg gcg
//
LOCUS       DAOSXM010000137         3867 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2111
            NODE_16421_length_3867_cov_9.498951, whole genome shotgun sequence.
ACCESSION   DAOSXM010000137 DAOSXM010000000
VERSION     DAOSXM010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864178
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3867)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3867)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:24:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,261
            CDSs (total)                      :: 2,229
            Genes (coding)                    :: 2,218
            CDSs (with protein)               :: 2,218
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 3 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3867
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16421_length_3867_cov_9.498951"
                     /isolate="AMR_MDS_2111"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..285
                     /locus_tag="PLQ68_10855"
     CDS             <1..285
                     /locus_tag="PLQ68_10855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXK73194.1"
                     /translation="TGCAHNGIMNIMEHYVELKGHMPQFVIGGLHLQNKTYTPTKEDD
                     FMKEITEYMLKSKAVFYTCHCTGQVAFDILKKDLKDKIHYLCTGKEMNIY"
     gene            296..1132
                     /locus_tag="PLQ68_10860"
     CDS             296..1132
                     /locus_tag="PLQ68_10860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018305249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HXK73195.1"
                     /translation="MNKECDNNCENCKSENTEKKNTKTDFSVNMHNLSSIKKVIAVVS
                     GKGGVGKSMVTSMLAVLMKRQGYHSAVLDADITGPSIPKAFNIKGRVSGSDLGMYPLK
                     SKTGIDIMSINLLLEHDTDPVVWRGPMLGNVVKQFWSEVIWTDIDYMFIDMPPGTSDV
                     PLTIFQSLPIDGIIVVTSPQELVSMIVEKAVKMANMMNIPVLGIVENYSYFLCPDNNK
                     KYKIFGESNIDEIAKQHEIKVMGKIPIDPRISTASDKGMIELFEGDWLDDAVEYLSKM
                     ED"
     gene            1136..1474
                     /locus_tag="PLQ68_10865"
     CDS             1136..1474
                     /locus_tag="PLQ68_10865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012062707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HXK73196.1"
                     /translation="MKIAVACEQKRVTEHFGHCENFSIFDVENNKIIKEDSVPNPGHR
                     PGFLPKFLAEKGVNVIMSGGMGGGAVDLFNEYGINVLTGVTGDAKDAVEQYLKGTLEY
                     SGSICKEHNH"
     gene            complement(1495..2961)
                     /locus_tag="PLQ68_10870"
     CDS             complement(1495..2961)
                     /locus_tag="PLQ68_10870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013322371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HXK73197.1"
                     /translation="MGEKDLRKLTLLHSNDMHGDFMSEKIDDRLVGGVSMLSGYIDKV
                     RNEEDNVAYVIAGDMFRGSIIDSEYKGISTIEIVNMLSPDVVTLGNHEVDYGIAHLLF
                     IEKLAKFPIINCNLYIKTNYARLFKPYFIEEVGGIKILYIGILTEDVIAQTKQESLIG
                     SIVDVYEAAQQVGKICNTYNKTDVDLTVLLTHIGHEEDKKLASILDPSWGVDIIIGGH
                     SHTFMEQPECINGILIAQAGTGSDQVGRFDLVIDKDNNAIHDWKWEAVPIDNSHCPID
                     KELEDIILNYHEKTHEKFARIITRFSHTLTHPQRNRETELGNLCADIMADNLGLDVML
                     FASGSIRKQELGPIVTFKDFKECFPFDDKIFRIKLNKSQVEKVFETIFGRDFINTHTE
                     FYQVSKTLKVRYSLKDKKIINLTLDEQPLDDHHLYSVGLQEYHFNNITDFLGITKQEV
                     EKNSPIRVVSTSCCDVLEEAFIASRLLISKVEGRITFE"
     gene            complement(2984..>3867)
                     /locus_tag="PLQ68_10875"
     CDS             complement(2984..>3867)
                     /locus_tag="PLQ68_10875"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HXK73198.1"
                     /translation="IMVYKSNEIAKMLSTVTNVFDVARIVDPKKWKILVFDTEGNITF
                     DEYSCYSIWEKEKRCLNCISIRALNEKKRFTKYEFVDDQTYYVVSKPLEIEDNERIIH
                     ECNLEIVANITDEVFFNAVGKNEFVDKILKSEKLQYIDSLTKAYNRRYYEEKIYLTYD
                     TDNFSKNVAFIMTDLKKFKLINDNYGHDAGDEVLKSVSRVYISNVRDKDSVIRFGGDE
                     FLIIINDCTFEIANEIVERINQSLKDVIYDTDKGLHVISNFGVAYTENFTNNDTLIAS
                     LLKEADKRMYEDKNKIS"
BASE COUNT         1317 a          663 c          587 g         1300 t
ORIGIN      
        1 acaggatgtg ctcataacgg aataatgaat attatggagc attatgtgga attaaaagga
       61 catatgccac aatttgtgat aggaggtctg catttacaaa acaaaacata cacaccaaca
      121 aaagaggatg atttcatgaa agaaattaca gagtatatgt taaaatcaaa agctgttttc
      181 tatacatgtc attgtaccgg acaagttgca tttgatatac ttaaaaaaga tttaaaagac
      241 aagatacatt atttatgtac cggtaaggaa atgaatattt attgaggaga ataagatgaa
      301 taaggaatgc gataataatt gcgaaaattg caaatctgaa aatacagaaa agaaaaatac
      361 aaaaactgat ttttcggtta atatgcataa tttgagcagt atcaaaaaag taatagcagt
      421 agtaagcgga aaaggcggtg ttggaaagtc catggtcacc tctatgcttg cagtactcat
      481 gaaaagacaa ggatatcatt cagcagtact tgacgcagac ataaccggac cttcaattcc
      541 taaagcattt aatataaaag gcagggtttc aggaagtgat ttgggaatgt accctttgaa
      601 aagcaagact ggtatcgata taatgtcgat taatcttctt ctagaacatg acaccgatcc
      661 tgttgtatgg agaggcccta tgttaggcaa tgtagtaaaa caattctggt ctgaggtcat
      721 atggactgat atagactata tgtttattga tatgcctcct ggaacatcag atgtgccttt
      781 aacaatattt caatctttac ctattgatgg aattattgta gtaacttccc ctcaggaact
      841 tgtttcaatg attgtagaaa aagcagtaaa gatggctaat atgatgaata taccggtttt
      901 gggaatagta gaaaattatt catacttcct ttgtccagat aataataaga aatataaaat
      961 tttcggagaa agcaatattg atgaaattgc aaaacagcat gaaataaaag ttatgggtaa
     1021 aatacctatt gacccaagaa tatcaaccgc ttcagataaa ggtatgattg agctttttga
     1081 aggtgactgg cttgatgatg cagtagaata tttaagtaaa atggaggatt aatttatgaa
     1141 gattgcagta gcatgtgaac agaaaagagt aacagaacat ttcgggcatt gtgaaaactt
     1201 tagtatcttt gatgtagaaa acaataaaat cattaaagaa gattcagtac ccaatcctgg
     1261 acacagacca ggtttcttac ccaaattcct agcggaaaag ggcgtcaacg taataatgtc
     1321 cggaggaatg ggcggaggtg ctgtggattt atttaatgag tatggaataa atgtacttac
     1381 tggagtaact ggagatgcca aagatgccgt cgaacaatac cttaaaggaa cattggaata
     1441 ctcaggttca atatgcaaag agcataacca ctgataatat tacttaaata catattattc
     1501 gaaggttata cgcccttcta cttttgaaat aagaagtctt gaagcaataa aagcttcttc
     1561 aaggacatcg caacaggaag ttgaaacaac tctaataggc gagttttttt ccacttcctg
     1621 ttttgttatt cctaaaaaat cagttatgtt attaaaatgg tattcctgta accctacaga
     1681 ataaaggtga tgatcatcta aaggctgttc atctaatgta aggtttataa tctttttatc
     1741 tttcaatgaa tatcttactt tcaatgtttt tgatacttga taaaactcgg tatgagtgtt
     1801 aataaaatct cttccaaaaa ttgtttcaaa aactttttca acctgagact tattcagttt
     1861 aatccggaat atcttatcat caaatggaaa acattcttta aaatccttga atgtgacaat
     1921 aggacctagt tcctgctttc taatactgcc tgatgcaaaa agcattacat ctagtcctaa
     1981 attatctgcc attatgtccg cacacagatt acctaattca gtttctctat ttctctgagg
     2041 atgggtaaga gtatgagaga aacgtgtaat tatccttgca aatttttcat gagttttttc
     2101 atgataattt aatataatat cttcaagttc cttatcaatc gggcaatgtg aattgtcaat
     2161 tggaacagct tcccatttcc aatcatgaat agcgttatta tctttatcaa taacaagatc
     2221 aaatctgcct acctggtcag aaccagtccc tgcctgagcg attaatatac cattaatgca
     2281 ttccggttgt tccataaatg tgtgtgaatg accgcctatg ataatatcaa caccccagga
     2341 tggatctagt attgaagcaa gctttttatc ttcttcatgt cctatatgcg ttaacaaaac
     2401 agtcaaatct acatctgttt tattatatgt attgcatatc ttccctactt gctgagctgc
     2461 ttcataaaca tcaacgatag agccgataag actttcctgt ttagtctgag caattacatc
     2521 ttcagtaagt attccgatat acaagatttt aataccccca acctcttcaa taaagtatgg
     2581 tttaaataat cttgcataat tagtttttat atacaggtta cagtttatta ttggaaattt
     2641 tgctaatttt tcaataaata acagatgagc gattccataa tctacttcgt gatttcccaa
     2701 tgttactaca tcaggtgata acatatttac gatctcaatt gttgatatgc ctttgtactc
     2761 agaatcaata atggaacctc tgaacatatc ccctgctatt acataagcaa cattatcttc
     2821 ttcgtttcga actttatcta tataccctga aagcatagac actccgccta caagacgatc
     2881 atctattttt tcactcataa agtcaccatg catatcatta gaatgcaata atgtaagctt
     2941 tcttaaatcc ttctcaccca tttcaatacc cccgcttata gaatcagctt attttattct
     3001 tatcctcata catcctctta tcagcttctt taagtaaaga tgctattaaa gtatcgttat
     3061 ttgtaaaatt ttcagtatat gcaactccga aatttgaaat tacatgaagt cccttgtctg
     3121 tatcatatat tacatctttt agggattgat taattctttc tactatttca tttgctattt
     3181 caaaagtgca gtcattaata ataataagga actcatcccc accaaatcta attacggaat
     3241 ctttatctct tacatttgat atatatactc tagaaacact tttaagaact tcatctcctg
     3301 catcatgacc ataattatca ttaattaatt taaatttttt caaatctgtc attataaatg
     3361 cgacattttt agaaaaatta tcagtatcat atgttaagta aattttctct tcataatatc
     3421 ttctattata tgcctttgta agagaatcta tgtattgaag cttttctgat tttaatatct
     3481 tatcaacaaa ctcatttttt ccaacagcat taaaaaatac ttcatctgta atatttgcaa
     3541 ctatttcaag attacactca tgaataattc tttcattatc ctctatctca agcggttttg
     3601 atactacata atatgtctga tcatcaacaa attcatattt agtaaatctt ttcttttcat
     3661 ttaatgccct tatacttata cagttcagac atctcttttc tttctcccat atactgtagc
     3721 acgagtattc atcaaaagta atattgccct ctgtatcaaa aactaaaatt ttccattttt
     3781 ttggatcaac tattcttgct acatcaaaca cattggttac tgttgatagc atcttagcaa
     3841 tctcattaga cttataaacc atgataa
//
LOCUS       DAOSXM010000138         3744 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2111
            NODE_17241_length_3744_cov_13.469233, whole genome shotgun
            sequence.
ACCESSION   DAOSXM010000138 DAOSXM010000000
VERSION     DAOSXM010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864178
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3744)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3744)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:24:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,261
            CDSs (total)                      :: 2,229
            Genes (coding)                    :: 2,218
            CDSs (with protein)               :: 2,218
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 3 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3744
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17241_length_3744_cov_13.469233"
                     /isolate="AMR_MDS_2111"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1218)
                     /locus_tag="PLQ68_10880"
     CDS             complement(<1..1218)
                     /locus_tag="PLQ68_10880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK73199.1"
                     /translation="MKTKKKIKDKEKEKDSEFKRDYSPKAILKTMTATIKMVQKLNNK
                     IVPLSFIRAFANSFYQLIAVLLSAKVIDLLIQKAAIKEIFIAVLLGVGGILVLNTIST
                     FIEKAIQINVAKCNVLFEFRTADKALDMDYDMLESPLATNIRLKIQEDRGWGSDINSI
                     SLQFYYIGRVTGGFVASIIMLWPLFTNANSLMTWDFFVIIISCTALTLTMRFILSPKW
                     WKKYDEITNEKSSMLKKAIFFTMDPSMMEYKSGKDIRIFAGQDLICSSIMPGIKERDE
                     KCNKLGLLHGMQHSTWSLSNVLAKALSFMYVGIKAAADVITLGGLIRYATGMYEFIMG
                     ICKIINVSTNFEYIAMLQKNTIDYLSIENKMFKGGLTIDKKEHCEYVISFINVSYKYP
                     GSEEYALRDFSLDL"
     gene            complement(1238..3079)
                     /locus_tag="PLQ68_10885"
     CDS             complement(1238..3079)
                     /locus_tag="PLQ68_10885"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXK73200.1"
                     /translation="MDKKHEKPKKVKPIYSLTSNILYAFRNVWETDRLITFLYIAALV
                     SVVILSYIEIYLPKVVISNVEMGNTAADIVLAVGIFALVHIVFQLLSDFSDCLTRERT
                     GKIWMRLLSKLFFKTLSCDYGKLEDPEGQNLYQRARTNLGQDRCIQQIYTTFFDIASA
                     ILCFILFSTVLSRLNFLIIFLLIGTSIANYYTLIAYEKWVQKNRLRWSEADKQQNYII
                     KSAGEYKFGKDIRIYSMKSWLVSLRDKIMIQQVKNCKERVRRSFIPDVVDILSTLLRD
                     GIAYGYLGYVAFEGRISIAEFALYFGAVNGLSAFVTNTIKNYYKLISQSIALSNVRQY
                     LDMPDPIDTDNPCSLNEITSFPEIKFVDVCFKYSNSDKWVLTNLNFTIKNGEKIALVG
                     INGAGKTTIVKLICGFYSPTIGTILIDGVNIERFKRADLFTMMSAVFQDISIFPFSIG
                     ENVSMKKLEETDYTLLEKCLKLAGLHEYVLKLEKGIETPLTRSIEENGHILSGGQQQK
                     LLLARSIYKASPILILDEPTAALDPIAESELYEKYMQLSADRTVIYISHRLASTRFCE
                     RIFFLQNGSITENGSHDELMRYKGKYAEMFEIQSHYYQKNIASEEIF"
     gene            complement(3247..>3744)
                     /locus_tag="PLQ68_10890"
     CDS             complement(3247..>3744)
                     /locus_tag="PLQ68_10890"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HXK73201.1"
                     /translation="DINELYYGSLGKYIHLTTSGDDFGMQNGPFLSPDMFKEMIAPWY
                     KKRISQVKSICNTLFFHHSCGSVYKLMDTIIDMGVDILNPIQPGTKDMEPEKLKAEYG
                     DKIVFWGGIDEQNLLSKATKEQVKEEVRRVSDILSKNGGYVLAASHNIQPDVPVENVD
                     AMLKA"
BASE COUNT         1087 a          717 c          662 g         1278 t
ORIGIN      
        1 taaatccagc gagaagtcac gcaaagcata ctcttcgctt cctggatact tatatgaaac
       61 attaataaaa gatattacat attcacaatg ctcttttttg tcgatggtta aaccgccttt
      121 aaacatttta ttctcaatag acagataatc tattgtgttt ttttggagca tggctatata
      181 ctcgaaattt gtcgacacat ttataatctt acatattccc ataatgaact catacattcc
      241 tgtcgcataa cgtataaggc ctcccaaagt gattacatca gcggctgctt tgatgcctac
      301 atacatgaat gataaggctt ttgccagtac atttgataaa gaccatgtcg aatgctgcat
      361 cccatgcaga agtccaagct tgttgcattt ctcatctcgt tcttttattc caggcataat
      421 gctgctgcat atgaggtcct gtcctgcaaa aatacgtatg tcttttccag atttatactc
      481 catcattgac gggtccattg tgaaaaatat ggctttcttc agcatagagc tcttttcatt
      541 tgttatctca tcatactttt tccaccattt cggactaaga ataaatctca tagtcaaagt
      601 aagtgctgtg catgaaatta ttattacaaa gaagtcccat gtcataaggg aattggcgtt
      661 tgtaaacaaa ggccacagca tgattattga cgctacaaat ccgccggtaa ctctgccgat
      721 atagtaaaac tgaagggaaa tggaatttat gtcagatccc catcctctgt cttcctgtat
      781 ttttaatcgt atatttgttg caagagggct ttccagcata tcataatcca tatccagtgc
      841 tttatcagct gtacggaatt caaaaagcac attacacttt gctacattta tctgtatggc
      901 tttttcgata aaagtagata ttgtattaag caccagaata cctccgacgc caaggaggac
      961 tgcaataaat atttctttta tcgctgcttt ctgtatgaga agatcaataa ccttagcaga
     1021 aagaagaact gctatgagct gatagaacga atttgcaaaa gctcgaataa atgacaaagg
     1081 aactatcttg ttgttcagtt tctgaaccat ttttatagta gcagtcatcg ttttgagtat
     1141 tgcttttggt gagtaatccc gcttaaactc tgagtccttt tctttttcct tatctttgat
     1201 cttctttttc gttttcatta aaatttgtcc tcctttctta gaatatttct tcgctggcta
     1261 tattcttctg ataataatgg ctttgaattt caaacatttc agcatatttc cctttgtatc
     1321 tcataagttc atcatggctt ccattttctg taatcgagcc attctgaagg aaaaatatac
     1381 gttcgcagaa acgtgtggaa gcaagcctgt gggaaatata tatgacagtt ctgtccgctg
     1441 acaattgcat atatttttca tacaattcgc tttcagcaat agggtcaagc gccgctgtcg
     1501 gctcatcaag tatcaggata ggggaagctt tgtatatgct tcttgctaaa agcagttttt
     1561 gttgctgtcc gcctgacaga atgtgtccgt tttcttctat tgaccttgta agaggggttt
     1621 cgattccttt ttccagtttc aacacatatt catgcagtcc tgcaagtttc agacactttt
     1681 ccaataaggt atagtctgtc tcttcaagtt ttttcattga tacattttca cctatagaga
     1741 atgggaagat agatatatcc tgaaatacag cagacatcat agtaaaaagg tcagctcttt
     1801 taaatctttc aatattcact ccgtcaatca gtatggtgcc gatggtaggt gaatagaatc
     1861 cgcatatcag ctttactatg gttgtttttc ctgcaccgtt aatgccgact aatgctatct
     1921 tctctccatt tttaatagtg aagttaagat ttgttagtac ccatttgtcc gaattagagt
     1981 atttgaagca gacatcaacg aatttaattt caggaaaact ggtaatctcg tttaaactgc
     2041 atggattatc agtatctatc gggtcaggca tatcaagata ctgccgcaca tttgaaagag
     2101 ctatgctttg tgatattagt ttgtaatagt tttttatagt attggttaca aatgcggata
     2161 aaccgtttac tgctccaaaa tatagagcga actcggctat tgatattctt ccctcgaaag
     2221 ctacataacc taggtatcca tatgctatgc catctcttaa aagggtagac agaatatcaa
     2281 cgacatcagg aataaacgaa cggcggacac gttctttaca gttctttacc tgctgtatca
     2341 ttattttgtc tcttaggctt acaagccagg atttcatgga atagatacgt atatcttttc
     2401 cgaacttgta ttcaccagca gatttgatta tgtaattttg ctgtttgtca gcttctgacc
     2461 agcgcagcct gttcttctgt acccattttt catatgcgat aagggtataa tagttagcaa
     2521 tggaggttcc gataagcagg aatatgataa gaaaattaag ccttgataat actgtagaga
     2581 aaagtataaa gcatagtatg gcagatgcta tatcgaaaaa tgtggtgtat atctgctgga
     2641 tgcatctgtc ctgacctaaa ttagttcttg ctctttgata aaggttctgg ccttcaggat
     2701 cttcaagttt gccataatca cagcttaatg tcttaaagaa cagtttgctt agtaagcgca
     2761 tccatatctt accggttctt tcacgcgtta aacagtcaga aaaatccgat aataactgga
     2821 atactatatg tacaagtgca aatatgccta cagcaagcac tatatctgca gcagtattgc
     2881 ccatctcgac attcgatata accacttttg gaagatatat ctctatatat gagagtatca
     2941 caacagatac taaagcagca atatagagaa acgttatgag acggtctgtt tcccagacat
     3001 ttctaaaagc atagagtata tttgatgtta agctatatat gggcttaacc tttttgggct
     3061 tttcgtgttt tttatccata ataattttcc taaattaaaa ggaatatatc atatagacat
     3121 attattgtca aaacaaatat ataaaatttt taaaacatat ataaattgtg tgcagtaata
     3181 gcaaagaaaa tatggtgtta tctatgtgct tttattactg cttttagcat tgtatgcttt
     3241 gagcagttat gctttgagca tggcatctac attctcaaca ggaacatctg gctgtatgtt
     3301 gtgtgatgca gctaagacat agccaccgtt tttgcttagt atgtcagata ctcttcgtac
     3361 ttcctctttg acctgttctt tagttgcttt ggataagagg ttctgctcgt caatgcctcc
     3421 ccagaagaca atcttatcgc catattcagc tttaagtttt tccggttcca tatcttttgt
     3481 cccaggctga atagggttca atatatctac tcccatgtca ataattgtat ccatgagctt
     3541 atacacagac ccgcatgaat gatggaagaa taaggtgttg catattgact taacctggct
     3601 gattctcttt ttgtaccagg gagctatcat ttctttaaac atatcaggtg aaaggaacgg
     3661 gccgttctgc atgccgaaat catctccgct tgtagtaaga tgaatgtatt ttcccaaact
     3721 tccgtaatac agttcattta tatc
//
LOCUS       DAOSXM010000139         3722 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2111
            NODE_17405_length_3722_cov_10.464685, whole genome shotgun
            sequence.
ACCESSION   DAOSXM010000139 DAOSXM010000000
VERSION     DAOSXM010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864178
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3722)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3722)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:24:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,261
            CDSs (total)                      :: 2,229
            Genes (coding)                    :: 2,218
            CDSs (with protein)               :: 2,218
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 3 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3722
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17405_length_3722_cov_10.464685"
                     /isolate="AMR_MDS_2111"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..500)
                     /locus_tag="PLQ68_10895"
     CDS             complement(<1..500)
                     /locus_tag="PLQ68_10895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK73202.1"
                     /translation="MKKISTNKLLSLLIVFALLLGLFPPVMLSVDAAAIVYRWDHAIN
                     VDDAFPYNDGFNGLNIWADDIGGNPRTLSSDSGWLKISGGTNNRLQIDKPDFSSFNNE
                     NFNVKIKSVSGTESVTVKFLDDSYSEISSRVTTVTATVEGAIASLPASSGVYCISFES
                     AGEFSIT"
     gene            complement(701..1855)
                     /locus_tag="PLQ68_10900"
     CDS             complement(701..1855)
                     /locus_tag="PLQ68_10900"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HXK73203.1"
                     /translation="MRDFELLKKMTKDFYTMKPDAPIALEEFGYYCKDEWIDKGYIKE
                     GEDMNKLFGFERLQNYYLPGLSGTVAPFIPRFKEEIIESDDLYEIAKDKAGRTVKYFK
                     GKRDGFMPQYLDFPVKDKKTWDDIKWRLDYASKERNEDLKRDIEKAIPVVQKGYHTVT
                     YIVGAFMYLRSLIGGEEILYMIYDDPQLIHTCMRKWLELSDYVTSKYQDHVRIDELLF
                     DEDICYNHGPLISPEQIRSFLFPYYKELIQGVRDRNRKFSISDKENVFKQSKGYDLII
                     HVATDGDCRPIIPVYKEIGMNYMSPMEVASGCDVVQIRKDHPDIRIKGGIDKRILAHS
                     KEAIKAEIDRILPFMKKHGGYLPTCDHGVPPEVPFENYVYFRELLKEYAK"
     gene            1996..2268
                     /locus_tag="PLQ68_10905"
     CDS             1996..2268
                     /locus_tag="PLQ68_10905"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HXK73204.1"
                     /translation="MTKLIYFKIPSCPFCRQADKFLEELMQENEQYRDIPIQIVDETS
                     QRALADSYNYYYVPTFYLGEKKLHEGVVTKNRVKSILDEYLKKSEE"
     assembly_gap    2307..2406
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2407..3008)
                     /locus_tag="PLQ68_10910"
     CDS             complement(<2407..3008)
                     /locus_tag="PLQ68_10910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK73205.1"
                     /translation="MKFVPDYMNISDAAKNKKAKRLPLYEHSISVTHMEKILNKNFAD
                     LINGDYKDKLEFFKWYCEFFKTMGYDTVSFEVAIRAVFPNGGALDKHVTPAIATREDL
                     LNYPWEEIPQLFHESSDESFRALKESLPSGMKMIGGPGYGIFECVQDLTGYMNLCVIS
                     YENHELYKDLFLNMRKLFVNIWKNFLQRHSDMCAVARMGD"
     gene            complement(3134..>3722)
                     /locus_tag="PLQ68_10915"
     CDS             complement(3134..>3722)
                     /locus_tag="PLQ68_10915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK73206.1"
                     /translation="KMKKIYLLTFILIMCLTLAACGTDKPSETATDTSTPSETATETA
                     TETPVEPVVLVDVVISGVAMKLPSGMTEMEDYFYADTTTADSATVTAGIADDSWPLSD
                     VSQDDFVGYQLYDRTNVVVSSFDNNIELNGNKALICKFSFTSEEGNGVSGAIVVVSYK
                     GAEYTVSLLYSSDNSEGALAASMDEVINSIAPVAY"
BASE COUNT         1116 a          706 c          536 g         1264 t
ORIGIN      
        1 gttatagaaa attcccctgc actttcaaaa gatatgcaat atacacccga tgaagcaggc
       61 aaactagcaa tagcaccttc gacagttgca gttactgtag taaccctgct tgatatttca
      121 ctatatgagt catccaagaa ttttactgta acagattcag ttccagaaac cgactttatt
      181 ttcacattga agttttcatt attaaaacta ctgaaatctg gtttatctat ttgcaaacga
      241 ttattagtac caccagaaat tttcaaccat ccgctgtcac tagataaagt ccttgggttt
      301 ccgccaatat catctgccca tatattcaga ccgttaaaac catcgttata tgggaatgca
      361 tcatctacat ttatagcatg atcccatctg tatacaattg ctgctgcatc cacactcagc
      421 attactggtg ggaatagtcc taataataga gcaaatacta ttaataatga taataacttg
      481 tttgttgata tttttttcat gagcacccct tttttttgca taagctacct aaaagcttaa
      541 tataagcttt caaataacag taatatgttt ctaatctcta catactaaaa gcaacatgtt
      601 ttttattatc ctataatcaa tacacgcatg tcaattattg ttaagaaatt tgtaagatta
      661 tttttacata ttagaattaa caatatttat aatttattaa ctatttagca tattctttta
      721 acagttcgcg aaaatacaca taattttcaa aaggaacttc cggaggcact ccatggtcac
      781 atgtcggaag ataaccgcca tgctttttca tgaaaggcag tatcctgtct atctcggctt
      841 ttattgcttc tttgctgtga gctaatattc ttttatcaat accgcctttt atacggatat
      901 cgggatggtc ttttcttatc tgcacaacat cacagcctga tgctacttcc ataggtgaca
      961 tgtagttcat gcctatctct ttgtatacag gaattattgg tctgcagtca ccatctgttg
     1021 cgacatggat tattaaatca tatcctttgg attgtttaaa tacattttcc ttatctgaaa
     1081 ttgaaaactt tctgtttcta tcccttacac cttgtattaa ctctttgtaa tatggaaaga
     1141 gaaaagaacg tatctgttct ggagatatta agggtccgtg attatagcat atatcttcat
     1201 caaaaagaag ttcatcaatc ctgacatgat cttgatattt tgatgttaca taatcagaaa
     1261 gctcaagcca ttttctcata caggtatgaa tcagctgagg gtcatcataa atcatataca
     1321 ggatttcctc tcctcctatc aatgacctta aatacataaa tgctccgact atatatgtta
     1381 cagtgtgata ccctttttga actactggta tcgctttttc tatgtctctt tttaaatcct
     1441 catttctttc tttactggca taatccagtc gccactttat atcatcccac gtttttttat
     1501 ctttcacagg aaaatccaga tactgaggca taaatccatc tcttttacct ttaaaatatt
     1561 taactgttct tccagctttg tcttttgcta tctcatataa atcatccgat tctataattt
     1621 cttctttgaa tctgggtatg aaaggagcta cagtacctga aaggcctgga agataatagt
     1681 tttgaagtct ttcaaagccg aagagtttgt tcatatcctc accttcttta atgtaccctt
     1741 tatctatcca ttcatcttta cagtaatatc cgaattcttc caaagcgatt ggagcatcag
     1801 gtttcatagt ataaaagtct ttagtcattt tctttagcag ttcaaaatct ctcatggtac
     1861 acctcggtta taatataact taaaaacagg tatattgtat atgtaaaaaa tttttttatt
     1921 atttttttct gatttttttt agtattgcca attttgatag tatataatta ttctatgaaa
     1981 ctttttggag gatttatgac taaactgata tatttcaaga taccatcttg tcctttttgc
     2041 agacaggcag ataaattttt agaagaacta atgcaggaaa atgaacagta tcgggatata
     2101 cctatacaaa tagtggatga aacctctcaa agagctcttg ctgacagtta taactactat
     2161 tatgtaccga ctttttatct aggagaaaag aagcttcatg aaggggttgt aactaaaaac
     2221 agggttaaaa gtattcttga tgaatatctg aaaaaatcag aagaataaaa ttagcctttt
     2281 ctatattccc taactatctc aatcatnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnnnnntcat cacccattct tgcgacagcg cacatgtcag aatgcctctg tagaaaattt
     2461 ttccatatat ttacaaacag ttttctcata tttaaaaaca aatctttgta aagctcatga
     2521 ttctcataag aaattacaca taagttcatg tatccggtaa gatcctggac acattcaaat
     2581 attccgtatc ctggtcctcc tatcatcttc atgcctgaag gcaaggattc tttgagagct
     2641 ctaaaggatt cgtcagaact ttcatgaaac aattgaggta tttcttccca tggatagttt
     2701 aaaaggtctt cccttgtagc aattgcagga gtcacgtgct tatccagtgc tcctccattt
     2761 ggaaatactg ctcttatggc cacttcaaaa gaaactgtat catatcccat tgtcttaaaa
     2821 aattcgcaat accacttgaa gaactccagt ttgtctttgt agtcgccgtt tataaggtct
     2881 gcaaaatttt tgtttaagat cttttccata tgggtaacac ttatgctgtg ctcataaaga
     2941 ggaagccttt ttgccttctt attctttgct gcatctgata tgttcatata gtccggtaca
     3001 aatttcataa tgcctcactt attatctttc agttctcttt tcaacattat atcaaacata
     3061 accaaacaat acagaatatt ttaaaaaacc ctccataact ggagggtgat tactcttttt
     3121 ctatacttag atgttaataa gctactggag ctatactgtt aataacttca tccatacttg
     3181 cagctaaagc accttcagag ttgtcagaag aataaagtaa gcttactgta tattcagctc
     3241 ctttatagct cacaacaaca attgctccgc taactccatt tccttcttca gaagtaaatg
     3301 aaaatttaca tatgagagct ttattgccat tcagttcaat attattgtca aaactgctta
     3361 caacaacatt agtcctgtca tataactggt atccgacaaa atcatcttgt gaaacatctg
     3421 ataatggcca gctatcatca gctataccag cagtaactgt tgcagaatca gcagtggtag
     3481 tatcagcata gaagtaatct tccatttctg tcattcccga aggtagtttc atggcaacac
     3541 ccgatatgac aacatcaact aaaaccacag gttcaacagg tgtttctgta gctgtctcag
     3601 tagctgtctc agaaggtgtc gaagtgtctg tggctgtttc gcttggtttg tcagtaccac
     3661 aagcagcgag agtcaggcac ataattaaaa taaaggtaag taaatatatc tttttcattt
     3721 tt
//
LOCUS       DAOSXM010000140         3709 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2111
            NODE_17501_length_3709_cov_9.434866, whole genome shotgun sequence.
ACCESSION   DAOSXM010000140 DAOSXM010000000
VERSION     DAOSXM010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864178
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3709)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3709)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:24:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,261
            CDSs (total)                      :: 2,229
            Genes (coding)                    :: 2,218
            CDSs (with protein)               :: 2,218
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 3 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3709
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17501_length_3709_cov_9.434866"
                     /isolate="AMR_MDS_2111"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            228..1031
                     /locus_tag="PLQ68_10920"
     CDS             228..1031
                     /locus_tag="PLQ68_10920"
                     /inference="COORDINATES: protein motif:HMM:NF013180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HXK73207.1"
                     /translation="MKNFIILTRAIEYIEQNLTEPLKREDIAKYCYVSLSCLEKLFSY
                     ALHTSIKSYIDKRIMTKAAKDIISSGYSITDIAMKYQYSSVEVFTRSFKRVWYVNPSQ
                     FKDNWKFTGIYPKIDYQYKKGDDLYMARKRVDISEAYDYLRSKKGSYVLCFDVKNLTQ
                     VNGISRKAGDLVILETASRIDKEAADDMLLLRIGGDEFALITGLNDEDSAHKLANKVI
                     AKNGQPVFFEERKIPLYLHCGMTIVPDQMRYSEFFKELHDTISNSKTNS"
     gene            1213..1959
                     /locus_tag="PLQ68_10925"
     CDS             1213..1959
                     /locus_tag="PLQ68_10925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012102935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HXK73208.1"
                     /translation="MDIDKLTIEEMKKGYRYDLNTGEYICNFCGKSYFEKEIYRFDDR
                     FYEASKAVAVHIDKEHDDIFDILVNKDTKYNTLTDNQKDLLRMMYVNSTDKQIAAKLE
                     ISSSTVRHMRFMFREKAKQAKMYLAIYELVNEKYTSQQERIIPIHSEATMIDDRYVTT
                     EKEKENIVKNSFSSLEPLVLKHFPVKEKKKIVVLAKISEQFENNRRYTEKEVNQVLKT
                     NFDDYVTLRRYLIEYGFMERTTDCNEYWLK"
     gene            1981..2328
                     /locus_tag="PLQ68_10930"
     CDS             1981..2328
                     /locus_tag="PLQ68_10930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012102934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HXK73209.1"
                     /translation="MDRKRELKEQYKNTKPDMGIIIIKSDVSNKCYLEATRRIKGAIN
                     KSIFTLDLGSHINKELQKDWNKFGKEHFTIQTLDELEYDEENPDKDYKKELEMLLDLW
                     KDKLIKEEAELYK"
     gene            2431..3636
                     /locus_tag="PLQ68_10935"
     CDS             2431..3636
                     /locus_tag="PLQ68_10935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK73210.1"
                     /translation="MMRGIGKFICILFIVTVLFASFSGCVTKQKLVYPSSSSSSLNSD
                     STGQEHQAEYSETSQSSIETDAEDDKLKIDIQPAEKLELELVKYNGGFFSIDLPSNWV
                     IETTGEYQNFGFHAYDPQNTDRSIFFYGNMRYFMKSEEGKAAWQTYMQFGGYSDAAVY
                     ADAPVLNPATTEQFFNTFDEYTVYAAKYGISHNFPEFENLQVLEKTPRNSPISSNCIE
                     DSILRVIFDSESGPCEGLFAAGVSNTMTAYMYNADAGYYTVYVITGISAPADEFIHLQ
                     ETLTKSIGSFSFTSSYIQQGVDLINQGTQLALDVGQAMSQSAQAYNDAWYNRDKVNDT
                     ISQKRSDTTLGYERVYDSSTGEVYRAEIGFFDEYDSNRDEYSNQGLQRVPDDGYDYYS
                     TPITGYVRK"
BASE COUNT         1360 a          512 c          711 g         1126 t
ORIGIN      
        1 gacacctggt gtactgactc tgtagttgca aaagatcagc ttgttttttc atattcaaag
       61 ggtaatccgg acattccgga tgaaacttat cagaaagttt atgataaatg gagaaatggc
      121 tgattccttt aactagaagt aaattatgct caaatcatca agccagaatc tgtatggctg
      181 atataatcgc attagtgaat gtattatgat gatataaggg gttaattttg aaaaacttca
      241 taatccttac cagagcaatt gaatatatag aacagaatct cacagaacct ttgaaaagag
      301 aagatattgc aaagtactgt tatgtgtcac tttcatgtct tgagaaactg ttcagttatg
      361 cattgcatac aagcattaag tcatatattg ataaacgcat aatgacaaaa gctgcaaagg
      421 atatcattag ttctgggtat tctattactg atattgcaat gaagtatcag tattcctcag
      481 tagaagtatt tacccgttca tttaaaagag tgtggtatgt gaatccttcg cagtttaagg
      541 acaactggaa gtttacaggt atctacccta aaatcgatta tcaatacaag aaaggagacg
      601 atttgtatat ggcgagaaaa agagtagata taagcgaagc atatgattac cttcgcagca
      661 agaaaggtag ttacgtgcta tgtttcgatg ttaaaaattt aacgcaggta aatgggattt
      721 ccagaaaagc cggagatctt gtgatactag aaactgcttc aaggatagat aaagaagcag
      781 cagatgatat gcttttatta agaatcggag gagatgagtt tgctttgatt acagggttaa
      841 atgatgagga ttcggctcat aagctggcta ataaggttat agctaagaat gggcagcctg
      901 ttttttttga agagcgtaaa attcctctgt accttcactg cggaatgaca atagtgcctg
      961 atcagatgcg ttatagcgaa ttcttcaaag aattgcacga tacgatatca aacagtaaaa
     1021 caaactcata aatcaataaa cagtgttgag atatacataa cagtatgcat atatgaatgc
     1081 aggtttactt acctgcattt atttttatta aaatatcaca ttgcagtgat ttatattaaa
     1141 tttatatgtt gacaattata ttaaacataa ataaaatatg tttataatat tcataaacat
     1201 aaggagatta cattggatat tgataaatta acaatagaag aaatgaaaaa aggttacaga
     1261 tatgatttaa acacaggaga atatatatgt aatttttgtg ggaaatcata ttttgaaaag
     1321 gaaatataca gatttgatga cagattttat gaagcatcaa aagctgtagc agtacatata
     1381 gacaaagagc atgatgatat ctttgatatt cttgtgaata aggatacgaa atacaatact
     1441 cttactgata atcaaaaaga tttattaaga atgatgtatg taaatagtac agataagcag
     1501 atagccgcta agcttgaaat atcttcttca actgtccgtc acatgcgttt tatgttcagg
     1561 gagaaagcaa aacaggcaaa aatgtatctg gctatatatg aacttgttaa tgaaaaatat
     1621 acatcacaac aggaaagaat aatacctata cacagcgagg ctactatgat agatgacaga
     1681 tatgtgacaa ctgaaaaaga aaaagagaat atagtcaaaa actctttctc cagtcttgaa
     1741 cctttggttc ttaagcattt cccggtaaag gagaaaaaga aaatagtagt actggcaaag
     1801 atttcagaac agtttgagaa caatagaaga tatacagaaa aagaagtaaa tcaggtatta
     1861 aaaactaatt ttgatgatta tgtcacttta agaagatatc tcatagaata tggatttatg
     1921 gaaagaacaa cagactgtaa tgaatattgg ttaaaataaa tatataaaaa ggaaaaagat
     1981 atggatagga aaagagaact taaagaacag tataagaata caaaacctga tatggggatt
     2041 atcataataa aatcagatgt tagcaataaa tgctatcttg aagctactag gcgtataaag
     2101 ggagctataa ataagagtat ttttactctt gatttaggaa gtcatataaa caaagagctt
     2161 caaaaagact ggaataaatt tgggaaagaa cactttacaa tacagacgct tgatgagctt
     2221 gagtatgatg aagaaaaccc ggataaggat tacaaaaaag aacttgagat gcttttagat
     2281 ttgtggaagg ataaacttat taaggaagaa gctgagttgt ataaataata tagtacttaa
     2341 cttaaggtaa ctctctttat ttttctgttg attcagtata aaattagtat agatgtaaga
     2401 ttattttaaa acaaaaaacg ggggttcttt gtgatgagag gcataggaaa atttatttgt
     2461 attcttttta tcgtaactgt tttatttgct tccttttcag gctgtgttac aaagcaaaag
     2521 cttgtatatc caagttctag ttcgtcttca ctaaatagtg acagtaccgg tcaggaacac
     2581 caggctgaat attctgaaac cagtcagtca agtattgaaa ccgatgctga agatgacaaa
     2641 cttaagatag atattcaacc tgctgaaaaa ctagagctgg aacttgtgaa atataatggc
     2701 gggtttttca gtatcgacct tccttcaaat tgggttattg agacaacagg ggaataccag
     2761 aatttcggtt ttcatgcgta tgatcctcaa aatactgata gatcgatatt cttttatgga
     2821 aatatgagat attttatgaa aagcgaggaa ggaaaagctg cttggcagac atatatgcaa
     2881 tttggaggct attccgatgc ggctgtatat gctgatgctc cagttttaaa tcctgctact
     2941 actgaacagt tctttaatac ttttgatgaa tatactgtat atgcagcaaa atatggtatt
     3001 agccataatt tccctgaatt tgagaatctt caggtattag aaaaaacacc aagaaattct
     3061 ccaatatcct ccaactgtat tgaagattca atattaaggg ttatatttga ctctgaatca
     3121 gggccatgtg aaggtttatt cgcagctgga gtatcaaaca ctatgacagc ttatatgtat
     3181 aatgctgatg ctggttatta tactgtttat gttataactg gaatatctgc accagctgat
     3241 gagtttattc accttcagga aacattgaca aaatcaattg gcagtttcag ttttacttcc
     3301 tcttatattc aacagggagt tgaccttata aaccagggta cgcaattagc gctagatgtc
     3361 ggtcaggcta tgtctcagtc agctcaggca tataatgatg cttggtataa tcgtgataaa
     3421 gttaatgata caatttcaca gaaaagaagt gataccactc taggttatga aagagtttac
     3481 gactcttcta caggcgaagt ttacagagct gaaataggtt tctttgacga atatgattcc
     3541 aacagagatg aatacagtaa ccagggtctt caaagagtac ctgatgatgg atatgattac
     3601 tattctacac caataacagg atatgttaga aaataaggcg ttataaggac aattagacag
     3661 cttcatttaa ataaaagttt gcatactcat attttgtggc tatataata
//
LOCUS       DAOSXM010000141         3688 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2111
            NODE_17655_length_3688_cov_10.096614, whole genome shotgun
            sequence.
ACCESSION   DAOSXM010000141 DAOSXM010000000
VERSION     DAOSXM010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864178
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3688)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3688)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:24:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,261
            CDSs (total)                      :: 2,229
            Genes (coding)                    :: 2,218
            CDSs (with protein)               :: 2,218
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 3 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3688
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17655_length_3688_cov_10.096614"
                     /isolate="AMR_MDS_2111"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..350)
                     /locus_tag="PLQ68_10940"
     CDS             complement(<1..350)
                     /locus_tag="PLQ68_10940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011916419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine synthetase N-terminal
                     domain-containing protein"
                     /protein_id="HXK73211.1"
                     /translation="MKKLFTSESVTEGHPDKLCDQISDAILDEILKQDSEARVACETA
                     VTTGLIHVMGEITTNCYVDIAKIARDTALTAGYTRAKFGFDGETCGVITSINEQSQDI
                     ALGVNSSYDMAGEQG"
     regulatory      complement(405..488)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(514..2349)
                     /gene="ftsH"
                     /locus_tag="PLQ68_10945"
     CDS             complement(514..2349)
                     /gene="ftsH"
                     /locus_tag="PLQ68_10945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010681207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HXK73212.1"
                     /translation="MKKYKNISFYVITFIILVILISLFTNQDSVKELGYSEFLNQLEK
                     QNVSEVVIRTNTLSITLKEPIKDGTTAFFVNVENLDKIEDILNDARKTDPDLSYTWKP
                     ETTSWWLSLLPSFIILAVVVVIFFVFMNKQAGANNSAMSFGKSRAREYTDKDRRVTFL
                     DVAGADEEKAELEEVVDFLKQPEKYKKLGARIPKGMLIVGPPGTGKTLLARAVAGEAG
                     VPFFSISGSDFVEMFVGVGASRVRDLFTQAKAKAPCIIFIDEIDAVGRHRGAGLGGGN
                     DEREQTLNQLLVEMDGFTIDQGIIILAATNRPDILDPALLRPGRFDRRVYVNYPDAKG
                     RYEILLVHTRNKKLDEDVDLKEISKITPMFTGADIENLMNEAAILAAKRDKEKISMEE
                     IRESIYKVAMGPEKKSKIVSEGERRLTAYHESGHAITMRTVSSTSKVQRVSIIPVGQA
                     GGYTMPMANEDKYFYTKQQLEEEIITLLGGRAAEEISIGEISTGASNDLQRANKIARN
                     MIVKYGMSENLANIVFQEDDEVFVGKDFSKVKGYSEEVASKIDHETKMIIDDCYMKVK
                     KIINEKREVLEILALSLLKNEKVEASEFERIYNGENPFEVEAQLT"
     gene            complement(2427..2972)
                     /gene="hpt"
                     /locus_tag="PLQ68_10950"
     CDS             complement(2427..2972)
                     /gene="hpt"
                     /locus_tag="PLQ68_10950"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012995990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HXK73213.1"
                     /translation="MSVLYDDVSEILITKEKLDEICGQLGEKISHDYKGKKLILVGIM
                     KGGLIFLSDLLRKISIPVEFGTVTASSYGQGTSSSGQVKILQDLDMDIRGKDVIIVED
                     LIDTGHTLKYLQNFFALRGPTSVKICCMLDKPSRREKQVHVDYIGQEIPDKFVVGYGM
                     DFNEKYRNLADVCVLKEEMYK"
     gene            complement(2969..>3688)
                     /gene="tilS"
                     /locus_tag="PLQ68_10955"
     CDS             complement(2969..>3688)
                     /gene="tilS"
                     /locus_tag="PLQ68_10955"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:NF023162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HXK73214.1"
                     /translation="NSMASLLKETEEYIIIQTDKEYEKRVNVTNSTVIIDTKNFDNVE
                     NVIKGEIVRKAINQVSGKLKDVEKKHIELILKLISNSQSGSIMDIKDGVKILVLQNSR
                     VKIFKDETEPVSFNQAVSIPGTTDVFKKNLSISAKIINYTSGSYMGKDWVILDYDSVV
                     NGLVIRNRQQKDKITPHKGNGTKTLKKYLIDNKVDNDIRDKLFILAIENEIVYIENKE
                     IGKRFIPKKGQIALRLEFKTI"
BASE COUNT         1030 a          751 c          501 g         1406 t
ORIGIN      
        1 ccttgttctc ctgccatatc ataagaacta ttaactccta aagctatatc ttgagattgt
       61 tcattgattg atgttattac accacaggtc tcgccatcaa agccaaattt ggctcttgta
      121 tagcctgctg tcaaagctgt atctctggca atctttgcta tatcaacgta gcaatttgtt
      181 gttatctcac ccattacatg tataagccct gttgtgactg cagtttcaca tgctactctt
      241 gcttcactgt cctgtttcaa tatttcatca agtattgcgt cagatatctg atcacatagt
      301 ttatctggat gtccctctgt tactgattct gatgtaaata attttttcat gttcattatt
      361 aactccttta ctataaaaaa ggcctcttga tgagaccttg tatatcctca tctgtttgga
      421 attggcacct ttgcttagca ggttgccggg cgtcatcgag ccagtctctc agccactctt
      481 gataagtacc tatattatat tttattgaag cttttatgtt aattgagctt caacttcaaa
      541 cggattttct ccattatata ttctttcaaa ctctgatgct tcaacttttt cattctttaa
      601 aagagataaa gcaagaattt caagaacttc tcttttttca ttaatgattt tttttacttt
      661 catataacaa tcatcaatta tcattttcgt ttcgtgatct atcttacttg caacttcttc
      721 agaatatccc tttactttag aaaagtcctt tccaacaaaa acttcatcat cttcttgaaa
      781 tacaatattt gctagatttt cactcatgcc atattttaca atcatatttc tggcaatttt
      841 attggctctc tgaagatcat tggaagctcc tgtacttatt tcaccaatgg atatttcttc
      901 tgctgcacgt ccgcctaaaa gagtaattat ttcttcttct aattgctgtt tggtataaaa
      961 atatttatct tcattagcca taggcattgt ataaccgcct gcttggccga ctggaataat
     1021 agaaactctc tgaacttttg atgtagaaga tactgtcctc attgttattg catgtcctga
     1081 ttcgtgatat gcagtaagtc ttctttcacc ttcagataca atcttgctct ttttttctgg
     1141 tcccatggca actttgtaaa ttgattctct tatctcttcc atggatattt tttctttatc
     1201 cctttttgct gctaatattg cagcttcatt catgagattt tcaatatcag caccagtaaa
     1261 cataggtgta atttttgata tctctttaag atcaacatct tcatcaagtt tcttgtttct
     1321 tgtgtgaaca agaagaatct catatcttcc ttttgcatca ggatagttaa cataaactct
     1381 tctatcaaat cttccaggac gaagtaatgc cggatcaaga atgtcaggtc tgtttgttgc
     1441 agcaagtatt ataatcccct gatcaattgt aaagccatcc atttcaacaa gtaactggtt
     1501 aagagtttgt tctctttcat cattgccacc accaagtccg gctcctctgt gtcttccaac
     1561 agcatctatt tcgtctataa atatgataca aggagctttt gcttttgcct gagtaaaaag
     1621 atcccttacc cttgatgctc ctacacctac aaacatttca acgaaatcag atccgcttat
     1681 gctgaaaaat ggtactccgg cttctcctgc aacagcacga gctaataagg ttttacctgt
     1741 tcctggaggt cctacaataa gcatgccttt gggtattctt gcaccgagtt ttttgtattt
     1801 ctctggctgt ttgaggaaat caacaacttc ctcaagctct gctttttctt catctgcgcc
     1861 tgctacatct aagaatgtaa ctcttctgtc tttatctgta tattctctgg ctctgctttt
     1921 accaaaagac attgctgagt tatttgctcc tgcttgctta ttcatgaata caaagaaaat
     1981 tacaacaacc acggcaagta ttataaatga gggcagtaat gaaagccacc atgatgtagt
     2041 ctctggtttc catgtatatg ataaatcagg gtctgttttt cttgcatcat taagaatgtc
     2101 ttcaatctta tccagatttt caacattcac aaagaaagca gttgtcccat cttttattgg
     2161 ttcttttaac gtaatagata atgtattggt gcgaatcact acttcagata cattctgttt
     2221 ctcaagttga ttaagaaatt cagaatatcc aagttccttt acagaatcct gatttgtaaa
     2281 taatgatatc aatatcacca gtattataaa tgttattaca taaaagctaa tgtttttgta
     2341 ctttttcaaa agtcatctcc ttttacctga taaaacatat tagcatataa aggctattaa
     2401 tgcaataaag ttcacataaa cttcatttac ttatacattt cttctttaag aacgcataca
     2461 tcagcaagat ttctgtattt ctcattgaaa tccataccat atcctactac gaacttatca
     2521 ggtatctcct gacctatata gtctacatga acttgtttct cgcgtcgtga tggtttatca
     2581 agcatgcagc atatttttac gctggtagga cctcttaatg caaagaaatt ttgaagatat
     2641 tttagtgtat gtccggtatc tataaggtct tcaacaataa taacatcctt acctcttata
     2701 tccatatcaa ggtcttgtaa tatctttact tgtccacttg aagatgttcc ctgtccataa
     2761 cttgatgcag taactgtacc aaattcaaca ggaatgctta tttttcttaa aagatcggat
     2821 aagaatataa gtccaccttt cataatccct acaagtatca gcttcttgcc tttataatca
     2881 tgagaaattt tttctcctaa ttgtccgcat atttcatcaa gcttctcttt tgttataaga
     2941 atttcactta catcatcgta taatacactc atattgtctt gaactccaat ctcaacgcaa
     3001 tttgtccttt tttaggtata aaccgtttac cgatttcctt gttctcaata tacactatct
     3061 cattttcaat cgcaagaata aaaagcttat ctcgaatatc attatccact ttattgtcaa
     3121 ttaaatactt tttcagagtt tttgtaccat ttcccttatg tggagtaatt ttatcttttt
     3181 gttgtctgtt tcttataaca agcccattta caactgaatc ataatcaagt attacccagt
     3241 ccttccccat atatgagcct gaggtatagt ttataatctt tgctgaaata cttaagtttt
     3301 ttttgaatac atcggtcgtt ccggggatag atacagcttg attaaaagag acaggttctg
     3361 tttcatcttt aaatatttta actcggctgt tttgcaaaac aagaatcttt acgccatctt
     3421 ttatatccat tattgaaccg ctttgactat tcgatataag tttaagtata agttcaatat
     3481 gttttttttc aacatccttt aattttccac taacctgatt tatagctttt cttactattt
     3541 cgccttttat aacattttct acattatcga aattcttagt atcaattata acagtgctat
     3601 ttgtaacatt aactctcttt tcatactctt tatcagtctg tataataata tattcttctg
     3661 tttctttcaa caaagaagcc atgctgtt
//
LOCUS       DAOSXM010000142         3606 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2111
            NODE_18233_length_3606_cov_10.553084, whole genome shotgun
            sequence.
ACCESSION   DAOSXM010000142 DAOSXM010000000
VERSION     DAOSXM010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864178
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3606)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3606)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:24:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,261
            CDSs (total)                      :: 2,229
            Genes (coding)                    :: 2,218
            CDSs (with protein)               :: 2,218
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 3 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3606
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18233_length_3606_cov_10.553084"
                     /isolate="AMR_MDS_2111"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(298..1701)
                     /locus_tag="PLQ68_10960"
     CDS             complement(298..1701)
                     /locus_tag="PLQ68_10960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK73215.1"
                     /translation="MINIGKRREVFFDDALIDTEKTAASKKLHECIRRDLAILHDEPW
                     EGDCSNYHNLFKDENGIYRMYYLGWSFGRKGICVCYAESHDGILWIKPKLGICEYNGS
                     FENNIILDPSTAIFDNFMVMKDPNQLKGSEELYKAICSIKSEKSKKYELWCFTSADAI
                     HFKKAWMMTDKGAFDTLNTVLWDKYTKKYYAYIRDFHDKEGCKPIRDIRVMTSDDFHK
                     WTIPVQLDFKGSNDYPLYTNVIQKYYRADHVFVGFPSRYVERPQWNSNFERLCGKEKR
                     LERMKTEPRFGLTVTDCVFMCSRDGMKWDRTDEAFMRPGPEEPYNWVYGDCYPSVGMV
                     ETKGSYPGTDNEISVYSYNNHWSNTPAKLWRYTIRLDGFVSRNASYKEKKLVTKPFIY
                     KGKSLHINFSTSAIGYIYITLHSEEGKSITSCELFGDSTNRIVDFEDGSVSDFQGKSV
                     VMEIRMSDADLYSFIFE"
     gene            complement(1744..2298)
                     /locus_tag="PLQ68_10965"
     CDS             complement(1744..2298)
                     /locus_tag="PLQ68_10965"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010800140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HXK73216.1"
                     /translation="MTEKEKSHAGILYQPGDPELVADRDVTIQKLYEYNKIHPLDRDA
                     KKAAIKGILGSTGENCVVEQPLFCTYGYNTTVGDNFFLNVNCKLMDSGKITIGNNVFI
                     APNVCIITEEHAMDVEQRIKGLEYTYPVNIGDNVWISTGALILPGVTIGANSVIGAGS
                     VVTKDIPPNSLAVGNPCKVIRKLK"
     gene            complement(2448..3092)
                     /locus_tag="PLQ68_10970"
     CDS             complement(2448..3092)
                     /locus_tag="PLQ68_10970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009259892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HXK73217.1"
                     /translation="MDNKTKKMTTIAMLCAIAYTVMVIGRIPVVMFLKYDPKDVIITL
                     GGFIWGPLTALVVSVIVSIIEMITVSETGILGCIMNIISTCSFACIASFIYKKRHTLP
                     GAVIGLVTGSLAMVSVMLLWNYLITPIYTGYPREQIVQLLLPVFLPFNLLKAGLNTGF
                     TFLLYKPIISSLRKGGYITTPDIKDRKKPMGLILLATAIIVTCVLIILAMNGVI"
     regulatory      complement(3186..3301)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(3465..3540)
                     /locus_tag="PLQ68_10975"
     tRNA            complement(3465..3540)
                     /locus_tag="PLQ68_10975"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3505..3507),aa:Pro,seq:tgg)
BASE COUNT         1088 a          720 c          534 g         1264 t
ORIGIN      
        1 tcagctttag taatattttt tgataaatca ttcttccaac tcatttttac cttctttctt
       61 tacttgattt atttaatcat tttttttgaa cagaaaaata agcaaacaca tttttgtaga
      121 actggcaaca ctatttttcg tttcatctct ttcgagttaa atctatattt cgatagttaa
      181 acgtaacaaa aagatatcta agcacctgaa taattaaatc agatactata ttatgtcaca
      241 tttaatgtaa tttgtatttt aacatatttt ggactttttg ccaatcaact aatagttcta
      301 ttcaaaaatg aaggaatata aatctgcatc actcatccga atctccataa caacactctt
      361 gccttgaaaa tctgaaacac ttccatcttc aaagtccact atacggttag tggagtctcc
      421 aaacagttcg caacttgtga tggattttcc ttcttcactg tgaagggtga tgtagatata
      481 cccaatcgct gaagtggaaa aattgatatg cagactcttc cctttataga taaatggttt
      541 tgtgaccagt tttttttctt tgtatgatgc attgcgtgat acaaacccat caaggcgtat
      601 ggtgtagcgc cagagttttg ccggagtatt gctccagtga ttattatatg agtaaacaga
      661 tatttcattg tcagtaccag gatagctgcc tttggtttcc accataccaa cagatggata
      721 acagtctcca tatacccagt tatatggctc ttccggtcct ggtcgcataa aagcttcatc
      781 agtacggtcc catttcattc cgtctcttga gcacataaat acacagtctg tgactgtaag
      841 accgaagcga ggttctgtct tcatgcgctc tagtcttttt tcctttccgc aaagtctttc
      901 aaaattgctg ttccattgcg gcctttcaac atatctgctt ggaaatccaa caaatacatg
      961 atctgctctg taatatttct gtatgacatt cgtgtatagt ggatagtcat tactgccttt
     1021 gaagtcaagc tgtacaggta ttgtccattt atgaaaatca tctgaagtca taacacgaat
     1081 atcacgaata ggtttacatc cctctttgtc gtgaaaatca cgtatgtatg cataatattt
     1141 ctttgtatac ttatcccata gaacagtatt taatgtatca aatgcccctt tatctgtcat
     1201 catccaggct ttcttgaaat gaatcgcatc agcacttgtg aaacaccaaa gttcatattt
     1261 tttactcttt tctgatttaa ttgaacaaat agctttatac agttcttcag aaccttttaa
     1321 ttgattagga tccttcatta ccatgaaatt atcgaagatt gcagttgaag gatcaagaat
     1381 aatattattt tcaaaagaac cgttatattc acatatccca agtttgggtt ttatccaaag
     1441 aattccatca tgactttcag cataacacac acatattcct ttccttccaa aactccaacc
     1501 cagatagtac atgcggtata tgccattttc atctttaaat agattatgat aattactaca
     1561 gtcgccttcc catggttcat catgtaatat agctagatcg cgtcgtatgc actcatgaag
     1621 tttttttgat gcggcagtct tctcagtatc aatcaatgca tcatcgaaga acacttctct
     1681 gcgcttacca atattaatca taattatcct ccagttatac ttatgctctt tgtatattac
     1741 tatttatttc agtttacgaa ttaccttgca tggatttcct acagcaagac tgtttggcgg
     1801 gatatccttt gtaactacac taccagcacc tataacactg tttgctccta ttgtaactcc
     1861 tggcagtata agcgctcctg tgcttatcca gacattatca ccaatattaa cagggtatgt
     1921 atattctaac cctttaattc tctgttctac atccattgca tgttcttcag ttataataca
     1981 gacatttggg gcaatgaata cattgtttcc tattgttatc ttcccgctgt ccatgagttt
     2041 acagttaaca ttaagaaaga agttatctcc aactgttgta ttgtatccat aagtacaaaa
     2101 aagaggctgc tccacaacac agttttctcc tgtacttcct aatatacctt ttatagcagc
     2161 tttttttgca tcacgatcca gtggatgtat tttattgtat tcatacaatt tctgaatagt
     2221 aacatcacgg tctgctacta gctcagggtc gccaggctga tatagtattc ctgcatgtga
     2281 tttttctttt tcagtcataa tgatttaacc tcgcatcatc taatgtaata gtaagaatta
     2341 tataattgta ttactctttt tacaattcat attactcaca tcattcattt ataacatcat
     2401 ctctttttaa aaattcagat atataaacat gtcttccaca tcatttatca aattactccg
     2461 ttcattgcaa gtataatcaa aacacatgta acaataatag ccgtcgcaag taaaatcaaa
     2521 cccattggct tttttctatc cttaatatca ggtgtagtaa tatagccgcc ttttcttaat
     2581 gaagatatga ttggtttata gagtaaaaat gtaaaacctg tatttaatcc tgctttcagt
     2641 aaattgaatg gaagaaacac tggaagtaac agctgtacaa tttgttctct tggataaccg
     2701 gtataaatag gtgtaataag ataattccat agaagcatta cagagaccat tgctaagctg
     2761 ccagttacta atccaataac tgcacctggt aaagtgtgcc ttttcttgta aataaatgat
     2821 gcgatacatg caaaagagca agttgaaata atattcataa tacaacctaa aatacctgtt
     2881 tcactcactg taatcatttc gattattgaa acaataactg atacaactaa agccgtcaat
     2941 gggccccata taaaaccgcc taaagtaata ataacatcct ttggatcata ttttagaaac
     3001 atcacaacag gaattctacc aataaccatt actgtatatg cgattgcaca gagcatagca
     3061 atagttgtca ttttttttgt tttgttatcc atcttgattt tcccccatat attattagca
     3121 cctgaaaggt ttagttttca ggtgctgata atggcgtaat tcaataattg gtttattgta
     3181 atattagtca tcttcttcta tccagactat actgttggtt ttggaattac accaaatcat
     3241 acgcctaacg ctcgcggact ataccgccaa tcgggaattt aaccctgccc tgaagacatt
     3301 ttattcagtt acttgtatca agtataatcc ggttgttaat gtatgacaat agaattagca
     3361 tatttttact atttcccctg cttattatat gttctttata atggctcatt cacagtataa
     3421 ataatcaatt taaaaaaaag acaccgattg tatcgatgtc tttctggtcg gagtgacagg
     3481 atttgaacct gcggcatcat ggtcccaaac catgcgcgct accaactgcg ctacaccccg
     3541 gtgctcatag tattttacta taccatcgag tagttgtcca tactattttt tatcttgttg
     3601 gtggat
//
LOCUS       DAOSXM010000143         3565 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2111
            NODE_18548_length_3565_cov_5.135328, whole genome shotgun sequence.
ACCESSION   DAOSXM010000143 DAOSXM010000000
VERSION     DAOSXM010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864178
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3565)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3565)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:24:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,261
            CDSs (total)                      :: 2,229
            Genes (coding)                    :: 2,218
            CDSs (with protein)               :: 2,218
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 3 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3565
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18548_length_3565_cov_5.135328"
                     /isolate="AMR_MDS_2111"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(163..1962)
                     /locus_tag="PLQ68_10980"
     CDS             complement(163..1962)
                     /locus_tag="PLQ68_10980"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HXK73218.1"
                     /translation="MHSHITYNPLQRETQKFVDKDCNVVVLAPTSSGKTIVAEQFLFP
                     AMKAGKKGLYLSPLKALTNEKLLDWEKTQFSVVAITSDHESKPRPITEDLILMTTEAL
                     DSRTRGIKQWLTEVGVIVCDEAHMLTVSGRGDAMEVGLTRFANEINRDARIIFLSATI
                     PNADELGEWLTVLNGKPTEVVTTEWRPIRQEHHLKTAKEKEWLFNDDAIDVIQRVQDK
                     YPDDQMIIFVHSIGKGKTIAQRLGVPFHYSKVSKADRQNLEKLFKEKKIKTLVSTSTL
                     AYGINLPADIGIIVGAHRGPTMVEAYDIIQMAGRVGRYGLSEKGLVYYVFKESYADMM
                     WNQITNMHPVESQLASRLYFHITSFVCRENKQIDDIKYFVSKTLLGQQQEVDVEESIN
                     KLLKYGILKQNEDGMLIPTLIGKASAMMYVDPIDLVTLRENLKSKPVNPRDIAIAFTN
                     IPSLDVPTYVPDDLKRKIDMGSGQQTILASALYSWLLGDDLFGTAAQVIPPYIADIER
                     IMAALKIAGVSKSYLDTIQIRLTYGVNENMIDLVSIRGIGRKRAFALSRLGINNREDM
                     LKKPTVVKNVLGTKLGMSVLAMIKDPTKPMIIY"
     gene            complement(1964..2461)
                     /locus_tag="PLQ68_10985"
     CDS             complement(1964..2461)
                     /locus_tag="PLQ68_10985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK73219.1"
                     /translation="MKGITCSKNGDDYVVSLHTQDELSADIHISEYFKDNPEFKKIAK
                     QSIDEQLDIPEDAQNSIDFLSEYTETPDLSEFNVIHIYSTGEYAAKENMGIPDAMFMK
                     IVGFSFKGLRKRTFKELCDDIRLYPNNHPELYQMKIFVDGSTLLFFRQPVSIMPGQTC
                     YIKEA"
     gene            complement(2458..3213)
                     /locus_tag="PLQ68_10990"
     CDS             complement(2458..3213)
                     /locus_tag="PLQ68_10990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK73220.1"
                     /translation="MKIPVVKHVIQPEWQGETVFIYGGGPSLRGQNLQPFLQGRKVLG
                     INTAFKLGDWISACLFGDTDFYVDEKNRVPLKQFKNPIYTMSDTAYKIGKSVDHTMRY
                     VAKDNRCIGMAPALNALCWHTSAGCYEMFDRLPDEEASIYRKLCHGTKSIPCGNTGAA
                     GIDLACKLGASCIVLLGFDMYHSNGQFNWHDEHTRHPADGKLILYSLRMAGIAYQLEQ
                     KDIKVVNTSMKSSIPWWKKQTLSEFLLTERRSK"
BASE COUNT         1078 a          732 c          521 g         1234 t
ORIGIN      
        1 tcatgctgct agctcaccat ctcaatcggc tcaaacctgt ctgtctgctc cacgtttgca
       61 ttcacattat ggatacagtg atagcatggt tcgtcggcat ccctcttgct tttgcatagg
      121 cgattgcttc gtccctctcc ttctttcagg caaatcatat ctttagtaaa ttatcatagg
      181 ttttgtaggg tcttttatca tggctagcac gctcatgcct agttttgtgc ctaatacatt
      241 cttaactaca gtaggttttt tgagcatatc ttcacgattg tttataccaa gacgactaag
      301 cgcaaaagca cgctttcgac ctatacctct aatagataca agatcaatca tattctcatt
      361 tactccataa gttaaccgta tctgtattgt atctaggtaa ctcttagaaa cacctgcaat
      421 ctttaaagca gccattatcc gttctatgtc cgctatatat ggtggtatta cttgagctgc
      481 tgtaccaaac aaatcatctc caagcaacca actataaaga gcagatgcta aaattgtttg
      541 ctgtcctgat cccatatcta tcttacgttt taaatcatca ggcacatatg taggaacgtc
      601 tagtgatgga atattagtaa atgctatcgc tatatctcta ggatttacag gcttactttt
      661 taagttttcc cgtaatgtta ctaagtctat tggatcaaca tacatcatag cagatgcttt
      721 accaattaaa gtaggtataa gcataccatc ttcattttgt ttcagaatac catactttaa
      781 caacttattt atagattctt ctacatcgac ctcttgctgt tgacctaaaa gagttttact
      841 aacaaaatat ttaatatcat ctatctgttt attctctcga cagacaaaag atgtaatatg
      901 aaaatataaa cgtgaagcta attgagactc tactggatgc atattagtta tttgattcca
      961 catcatgtct gcatacgatt ctttaaatac ataatacact aaaccttttt cagataatcc
     1021 atagcgacct actcgacctg ccatttgtat tatatcatac gcttctacca tagtaggacc
     1081 acgatgcgca ccaactataa tacctatatc agcaggtaaa tttataccat atgccagtgt
     1141 tgatgtagat accagagttt ttatcttttt ttctttaaac aacttttcaa gattttgtct
     1201 atctgctttt gatactttag aataatgaaa aggcacacca agtctctgag cgatggtttt
     1261 acctttcccg atagaatgta caaatattat catttgatcg tctggatatt tatcctgtac
     1321 tcgttgtatt acatcaatag cgtcatcatt aaaaagccat tccttttctt ttgctgtttt
     1381 aagatggtgt tcttgtctaa taggtctcca ttctgttgtt actacttcag ttggtttacc
     1441 attaagtaca gttagccact cacctaactc atcagcatta ggaatagttg ctgaaagaaa
     1501 tataattcta gcgtctcgat taatttcatt agcgaaacga gtaagaccga cttccatagc
     1561 atcgcctcga cctgaaacag taagcatatg tgcttcatca caaacaatta cacctacttc
     1621 tgtaagccac tgcttaatac ctcttgttct actatctaat gcttccgtag tcataagtat
     1681 caaatcttca gtaataggtc ttggtttaga ttcatgatca cttgttatag cgacaacact
     1741 aaattgtgtc ttttcccagt caagcaactt ttcattagta agagctttta aaggactaag
     1801 atacaaccct tttttacctg ctttcatagc aggaaataag aattgttcag caacaatagt
     1861 tttacctgat gaagtaggag caagcacaac tacattgcaa tctttatcta caaatttttg
     1921 tgtctctctt tgaagtggat tataggttat atgtgaatgc atattaagcc tccttaatat
     1981 aacaggtctg tccaggcatt atagaaacag gctgtctgaa aaacaaaagt gttgacccat
     2041 cgacaaatat cttcatttga tataattcag gatgattatt tggataaagt cttatatcat
     2101 cacaaagttc cttaaatgta cgtttacgca atcctttaaa tgaaaaacct acaattttca
     2161 taaacatagc atcggggata cccatattct ctttagctgc gtattcgcct gtggaataaa
     2221 tatgaattac attaaattca gacaaatcag gtgtttcagt atattcagat aagaaatcaa
     2281 tactgttttg tgcatcttct ggtatatcta gttgctcatc tatactttgt tttgcaatct
     2341 ttttaaactc aggattatcc ttaaaatatt cacttatatg aatgtctgca cttaattcat
     2401 cttgtgtatg caacgaaaca acataatcat caccattttt gctgcaagtt atccctttca
     2461 tttacttctc ctttctgtta aaagaaattc acttaatgtt tgctttttcc accatggaat
     2521 gctacttttc atcgacgtat taactacttt aatatctttc tgttcaagtt gatatgctat
     2581 acctgccatc cttaaactgt ataaaataag tttaccatcg gcaggatgcc tagtatgttc
     2641 atcatgccag ttaaactgtc cgttactatg atacatatca aatccaagca gtacaataca
     2701 tgaagcacca agtttacatg ctaaatcaat tccagctgca cctgtatttc cacaaggtat
     2761 gcttttagtt ccatgacata attttctata aatacttgct tcttcatcag gcaatctatc
     2821 aaacatttca taacatccag cagatgtatg ccagcataat gcatttaatg ccggtgccat
     2881 gccaatacat ctattatctt ttgcaacata tcgcattgta tgatctaccg atttaccaat
     2941 cttatatgcg gtatctgaca tggtatatat tggattctta aattgtttta gtggaacacg
     3001 attcttttca tctacataaa aatcagtatc tccaaataaa caagctgaaa tccagtcgcc
     3061 taatttaaaa gcagtattta tacctagaac cttcctgcct tgtagaaatg gttgtaggtt
     3121 ttggcccctc aaagagggac cgccaccata gataaatact gtttcacctt gccattctgg
     3181 ttgtattaca tgctttacta ctggtatctt catttcatgc acgaccttct aaagttaaga
     3241 aagttgtatc gtacaacata gagtccatcg ggacatttga ccgtataaac tcctttgccc
     3301 gcctccattt ttgggaaact tcattgtaag gcataccaag atatgcacta gcttgaatta
     3361 tagaacagtt gcactctaac atgatgtata ctatttctcg atgttttaag ttaacaggta
     3421 atacaattac gacttcttgt tttgcaaata cttgactatt ttcatcaaaa ctatgcttcc
     3481 aaaacttgta aatagcccta cgccctcgca acaaggaaat taacttatta tacattaaac
     3541 gcctgacata attatacatt atagg
//
LOCUS       DAOSXM010000144         3547 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2111
            NODE_18699_length_3547_cov_10.573024, whole genome shotgun
            sequence.
ACCESSION   DAOSXM010000144 DAOSXM010000000
VERSION     DAOSXM010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864178
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3547)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3547)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:24:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,261
            CDSs (total)                      :: 2,229
            Genes (coding)                    :: 2,218
            CDSs (with protein)               :: 2,218
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 3 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3547
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18699_length_3547_cov_10.573024"
                     /isolate="AMR_MDS_2111"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..436
                     /locus_tag="PLQ68_10995"
     CDS             <1..436
                     /locus_tag="PLQ68_10995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK73221.1"
                     /translation="ELVDVTTEQGINIKLPSDITLQTMNGVPYYANLETGDNVVFTVM
                     ETYEGLFNEFTEENVLALYQTNYSDTSLISFSNDTQINGKDALISKITTTTTDGNNVT
                     IAFVMVITDSTTYISNFTYGTNDNNSSLANNIDTCIESITIN"
     gene            584..931
                     /locus_tag="PLQ68_11000"
     CDS             584..931
                     /locus_tag="PLQ68_11000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019163035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HXK73222.1"
                     /translation="MLFICYSKCSTCKKAQQWLDDNGLEYSVRDIKTMNPSYSELKKW
                     VSGISVKKFWNTSGMLYRSLGIKDKLLTMNEDEQLKLLATDGMLVKRPILVTDNIVLV
                     GFKEKEWEAALNI"
     gene            1040..2428
                     /locus_tag="PLQ68_11005"
     CDS             1040..2428
                     /locus_tag="PLQ68_11005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013886717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXK73223.1"
                     /translation="MSRFIDREQELNFLLSEYRRSGSSLVVIYGRRRIGKTELISKFI
                     NDKKSIYFLATEEDEVQNRNAFKNTVSDFLGNELLREARIDNWEILFKSLVAKTTNEK
                     IILAIDEFQYLGLANSAFPSIMQKMWDTYLKNSNIMVILCGSLISMMESQTLNYSSPL
                     YGRRTGQIRLKQIPFLYYHDFYPNKDYKSLIEYYSVTGGVPKYIELFYDSEDIYSAIN
                     INILSKQSFLYDEAHFLLQKEVSEIGTYFSIMKAIAAGNRKLSKISSSIEIKQTGLSK
                     YLKTLASLDLIEREVPVTEPNPQKSKKGLYRIKDNYILFWFKFIYPNMGFIESGNTAI
                     VMNKLKENFIDKHVSFIYENVCIEKLWNINAEGSLKFTFDKIGRWWNNTSEIDIIGID
                     STGNNILFGECKYKKSKIGLDVLTDLEKKSNEVEWNKDNRKSYYIIFSISGFTEELIK
                     EAQKRNDVMLSE"
     gene            complement(2418..3455)
                     /locus_tag="PLQ68_11010"
     CDS             complement(2418..3455)
                     /locus_tag="PLQ68_11010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK73224.1"
                     /translation="MKQARIIFTILIITLCTCCTFTSVSACTAIAVYSDNTLYGFNFD
                     YPPVDMRFDICRYNNMKVFSTSFNRSNNYEPNLEFNEEGLFGVMLIVYPEEQGQTYLS
                     TNEIFMPALVSMVRTEDRTEDILKNIQERKVVQYANVTLHDIFADIHGNTVIIEAKGD
                     INNIIKNDKNFTVMTNFYNSSYKDTDLEDIQDVGSERYKIAYKYINENIDKFDVESAF
                     ECLSKVVQKPSFSSWPTQYSLVMDPVNKDIYFTIARDFDKVWKISLDNETIETYIGFD
                     EYTIAHMPINGFTLSELENSNLDNFKTYEQSKSNNTVYIITIVTGVLVLTAVIALILI
                     KKRKKSNTATH"
BASE COUNT         1217 a          526 c          582 g         1222 t
ORIGIN      
        1 tgagttagtt gatgtaacaa cagagcaagg tattaatatc aagcttccaa gtgatattac
       61 acttcagaca atgaatggtg taccatacta tgctaatctt gaaacaggtg ataatgtagt
      121 atttacagtt atggaaacat acgaaggctt gtttaatgaa tttactgagg aaaatgtatt
      181 agctttgtat caaactaatt attcagatac ttcattaatc agtttttcaa atgatactca
      241 gataaatgga aaagatgctc ttatatcaaa gataacaaca actacaacag atggaaacaa
      301 tgttactatt gcttttgtaa tggttattac tgattcaact acatacattt cgaactttac
      361 atatggaaca aatgacaata acagttccct tgctaataac atagatacat gtattgagag
      421 cataactatt aattaatagt tattgaatta gactatactt atttaaagag cctgaagcag
      481 gctctttttt aagtctttat tcctttatca gtttatcttt gatatattcc ttgttcttta
      541 tatatccttg tctttgatat aatgtagtta attggaggtt accatgttat tcatatgtta
      601 ttctaaatgt tctacatgta agaaagctca gcagtggctg gatgataatg gcttggaata
      661 ttctgttcgt gatattaaga ccatgaatcc ttcctattct gaattaaaga aatgggtcag
      721 tggtattagt gtaaagaaat tctggaatac ctcaggtatg ctatataggt ccttaggcat
      781 taaggataag cttctgacta tgaacgaaga tgaacagcta aaactactag caactgatgg
      841 aatgcttgta aaaagaccta ttcttgttac tgataatatt gtacttgtcg gattcaaaga
      901 aaaggaatgg gaagctgcac taaatatata gtccattatt tatttatctg tttgcttgat
      961 tatatccttt tattgattag taaattatta tacttgaaat tattatactt tggattataa
     1021 taaatattga ggtgatatca tgtcaaggtt tatagatcgg gagcaggaac tgaatttcct
     1081 tttaagtgaa tacagaagat ctggttcatc acttgtagta atatatggaa gacgaagaat
     1141 cggaaagaca gagcttattt ccaagtttat aaatgataag aaatctatat attttcttgc
     1201 tacagaggaa gatgaagtac agaacaggaa tgcctttaaa aatactgtgt ctgacttttt
     1261 aggtaatgaa cttttaagag aagcgcgtat cgataattgg gagatactat tcaaatcctt
     1321 agttgcaaaa acaaccaatg aaaagataat attggctata gatgaatttc agtatttagg
     1381 tcttgccaat tcagctttcc cttctattat gcagaaaatg tgggatacat acctaaagaa
     1441 tagtaatatt atggtgattc tatgcggttc acttatatca atgatggaaa gtcagactct
     1501 gaattattcc agtccgctat atggcagaag aaccgggcag ataagattga aacagattcc
     1561 tttcctttat tatcacgatt tttatccaaa caaggattat aaatcattaa ttgaatacta
     1621 ttctgtaact ggcggagtac ctaagtacat tgaactgttt tatgacagcg aagatatata
     1681 ttcggctatt aatataaata tactttcaaa acaatctttc ttgtatgatg aagcccattt
     1741 tctgttgcaa aaggaagtat ctgaaattgg tacatatttc tctattatga aagctatagc
     1801 agctggaaac cgaaaattgt ccaaaatatc cagcagtatt gaaattaagc agacaggctt
     1861 aagtaagtac ttaaaaactt tagcatcact tgaccttatt gaacgtgaag taccagttac
     1921 agaacccaat ccacaaaaaa gcaaaaaagg actgtacaga ataaaggata attacatact
     1981 cttctggttc aaattcattt accctaatat gggatttatt gaatctggga acacagctat
     2041 tgtaatgaat aaactaaaag agaacttcat tgataagcat gtaagtttca tatatgaaaa
     2101 tgtatgtata gaaaaattat ggaatattaa tgcagaagga agcttaaagt tcacttttga
     2161 taaaattggc agatggtgga ataatacatc tgaaatagat atcataggaa ttgacagcac
     2221 tggtaataat atattgtttg gagagtgcaa gtacaaaaaa agtaaaatcg ggcttgatgt
     2281 tcttactgac cttgagaaaa aatcaaatga agtggaatgg aacaaggata atcgaaaatc
     2341 atattacata atatttagca tttcaggatt tactgaagag cttattaaag aagcccagaa
     2401 aagaaatgat gtaatgctca gtgagtagct gtatttgatt tttttctttt ctttattaaa
     2461 attaaagcta taactgctgt taatactaat acacctgtga ctatagttat tatataaaca
     2521 gtattattgc ttttactttg ttcatatgtc ttgaaattat caagattact attttcaagt
     2581 tcagagagag taaacccatt tattggcata tgagctattg tatattcatc aaaccctata
     2641 tatgtttcta tggtttcatt gtctaatgat atcttccata ccttatcgaa atctcttgct
     2701 atagtgaaat atatatcctt atttaccgga tccataacca gcgagtactg tgtaggccag
     2761 gaggaaaatg aaggtttctg tacgacttta gataagcatt caaatgcaga ttcgacatca
     2821 aacttatcta tattctcatt aatatatttg tatgcgatct tgtatctttc agagccgaca
     2881 tcctgtatat cctctaaatc cgtatcctta tatgaactgt tatagaagtt tgtcataaca
     2941 gtaaagttct tatcattttt tataatattg tttatatctc cctttgcttc aataattacc
     3001 gtattaccat gaatatcagc aaatatatca tgaagcgtta cattagcata ctgtactact
     3061 tttctttcct gtatattttt taatatatcc tctgttctgt cttctgtcct taccatactg
     3121 actagagcgg gcatgaatat ttcattagta gacaagtatg tttgtccctg ttcttctgga
     3181 taaacaataa gcataacccc gaataaccct tcttcattga attccagatt cggttcataa
     3241 ttattgctac gattaaagga tgttgaaaag actttcatat tattgtatct gcatatatca
     3301 aacctcatat caacaggcgg ataatcaaaa ttaaacccat aaagtgtgtt atctgagtaa
     3361 acagctatag cagtacaggc tgatacagat gtaaacgtac aacatgtaca tagtgtgatt
     3421 atgaggattg taaatatgat ccttgcttgt ttcatgttaa tactacctct tactgctaat
     3481 attatcatac tccttaatta atgtttttgc atattttgct ataatcactt ttctatctaa
     3541 gtggtat
//
LOCUS       DAOSXM010000145         3418 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2111
            NODE_19776_length_3418_cov_9.535236, whole genome shotgun sequence.
ACCESSION   DAOSXM010000145 DAOSXM010000000
VERSION     DAOSXM010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864178
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3418)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3418)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:24:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,261
            CDSs (total)                      :: 2,229
            Genes (coding)                    :: 2,218
            CDSs (with protein)               :: 2,218
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 3 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3418
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19776_length_3418_cov_9.535236"
                     /isolate="AMR_MDS_2111"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1115
                     /locus_tag="PLQ68_11015"
     CDS             <1..1115
                     /locus_tag="PLQ68_11015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013273239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF438 domain-containing protein"
                     /protein_id="HXK73225.1"
                     /translation="EVKGLFEKEFENVSANEISAAEQALISEGVEVGEIQKLCDVHAA
                     VFKGSIEDIHKETDQTKLPGHPIYILKKENRIIETWIEKEIRPFLNASGNSYKQLIEG
                     MNRLSKIDIHYSKKENLFFPYMEKYGITAPPKVMWGVDDEIRGMIKNSISNLVMAGIV
                     TDEIRLLIESTIEKTLEMIYKEENIMLPMLIEALSLEDWKVIANESKEIGYLYENVPK
                     WNPVVKKEESAEELKSISGSIILPSGVFKVEELTHMLNTLPFDITFVDKDDKVKFFSQ
                     GEDRVFARTKAIIGRDVSNCHPPASVHVVQKIVEDFKSGKKDHEDFWIKMGDRYVYIR
                     YFAVRGDNNEYLGVVEVTQNIAPIQELQGEKRLMSD"
     gene            complement(1112..1948)
                     /locus_tag="PLQ68_11020"
     CDS             complement(1112..1948)
                     /locus_tag="PLQ68_11020"
                     /inference="COORDINATES: protein motif:HMM:NF015951.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HXK73226.1"
                     /translation="MKLKKPIPVLEWILLVIKGMVVGAGAILPGISGGVLCVVFGIYQ
                     PMMALLAHPIKSFKIYYKLFIPFLFGWVLGFLALAKLVEMLFAASSTIAISLFVGLIA
                     GTLPSLFKEAGKNGSSKGSWIGFSIGLFVMFTVLTMLSKGTATDISPNIWWFAFCGIV
                     WGFSLIIPGLSSSSILIFMGLYQPMTSGIASLDMLVILPLIAGLAITVFLFARLVNFL
                     FEKHHSIMHHTIIGIVIASTLLIIPVEYTSNVNLAISIACFFAGFIVSILMDKLENKI
                     SF"
     gene            2153..3406
                     /locus_tag="PLQ68_11025"
     CDS             2153..3406
                     /locus_tag="PLQ68_11025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006783835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HXK73227.1"
                     /translation="METIRTINYILSVIFLVCYSYQFVYIFISLFLKRKSLGTYEEKK
                     YAVLICARNEENVIGQLISSIHNQDYSKNLIDIYVAADNCTDDTAKIARQLGAIVYER
                     RNTEKVGKGYALNFLLCNIDSKYGIEEYAGFFVFDADNLLDESYISEMNKVFSKGFNI
                     VTSYRNSKNYDTNWISAGYSLWFLREACSLNHPRMAAKSSCAVSGTGFMFSKEVIRKC
                     GGWNFYLLTEDIEFTVYNIINNEKIGYCPTAILYDEQPVTFSQSWHQRMRWAKGYLQV
                     FSKYGLAMIKGIFKKTGFSCFDMCMSTMPAVFLTAFGIIINISAIFVGVLNGTDVTVV
                     LGSMVETFFNMYLMLFAIGFITTVTQWKMIHANSFKKILYLFTFPIFMMTYIPISLTA
                     IFKKVDWKPIKHTHCATLAQVRMKK"
BASE COUNT         1234 a          487 c          621 g         1076 t
ORIGIN      
        1 atgaagttaa aggtttattt gaaaaagaat ttgagaatgt atctgctaat gaaatatctg
       61 cagctgaaca agccttaata tctgaaggtg tggaagtagg agaaattcag aaattatgtg
      121 atgttcatgc cgcagttttt aagggttcca tagaagatat acataaagaa acagatcaga
      181 cgaaattgcc gggacatcct atatatattt taaaaaagga aaataggatt atcgaaacat
      241 ggatagaaaa ggaaataaga ccattcttaa atgcatcagg caattcttat aaacagttaa
      301 tagaaggtat gaacagatta tctaaaattg atatacatta ctcaaagaag gagaatttat
      361 tctttccata tatggaaaaa tacgggataa ctgcacctcc taaggtaatg tggggagtag
      421 atgatgaaat taggggtatg attaaaaatt caatatctaa tcttgttatg gcaggaattg
      481 taacagatga gataagactt cttattgaat ctacaattga aaaaacatta gaaatgatat
      541 acaaagaaga aaacattatg cttcctatgc ttattgaagc tttatcattg gaagattgga
      601 aggtaatagc aaatgagtca aaagaaatag gttatttata tgaaaatgtt ccaaagtgga
      661 accctgtagt taaaaaagag gagtcagcag aagaacttaa aagcattagc ggatcgatta
      721 ttcttccaag tggagtgttc aaggtggaag aacttaccca tatgctaaat acattgcctt
      781 ttgacataac attcgttgat aaggatgata aggttaaatt tttctctcaa ggagaagaca
      841 gagtttttgc aagaacaaaa gctattattg gcagggatgt atcaaactgt catcctcctg
      901 cttctgtcca tgtggtacag aagattgttg aagacttcaa atcaggtaaa aaagatcatg
      961 aggatttctg gattaagatg ggggacagat atgtgtatat aaggtatttt gctgtcagag
     1021 gcgataataa cgaatatctt ggtgttgttg aagttactca gaatattgcg cctatacagg
     1081 aattacaagg tgaaaaaagg ttgatgtcgg attaaaagga tattttattt tccagtttat
     1141 ccataagtat tgaaacaata aagcctgcaa aaaagcaggc tatacttata gctaaattta
     1201 catttgaagt atactcaaca ggaattatca ataaagttga tgcaatgact attccaataa
     1261 ttgtgtgatg cattatactg tgatgttttt caaaaagaaa attaaccagt cttgcaaata
     1321 agaatacagt tattgcaaga ccagcaatta atggtaaaat tacaagcata tcaagcgatg
     1381 ctatgccaga ggtcattggc tggtacaggc ccatgaaaat taatattgaa gaggaactta
     1441 atcctggaat aatcaaactg aaaccccata caattccgca aaaagcgaac caccatatat
     1501 ttggagatat atctgtagca gttcctttac ttagcatagt taatactgtg aacattacaa
     1561 aaaggcctat agaaaatcca atccatgatc cttttgaaga gccgttttta cctgcttctt
     1621 taaataatga aggtagtgtt ccagctatta ggccaacaaa taaagaaatt gctattgttg
     1681 aagaagctgc aaaaagcatt tctactagct tggccagtgc caaaaatccc aagacccagc
     1741 caaaaagaaa aggtatgaat aatttataat atattttaaa agatttaata ggatgtgcaa
     1801 gaagagccat cattggctga tatattccga atactacgca taatacacct ccgcttatcc
     1861 ctggaagtat agcacctgcc ccaacaacca ttccttttat tacaagtaat atccattcca
     1921 gtacaggaat tggctttttc agtttcataa ggataccttt cagaacttaa taatagaatt
     1981 ataccattta ctatttttag cacaatgtgt accagaatat tttataacac tttatagatt
     2041 taattattaa gagtttttta ataaatttta tctatattaa taaaatatta attattaaaa
     2101 cagttttttc ttaagattct ttcctatata attcctcaaa acggaggatt ttatggaaac
     2161 cataagaaca ataaactaca tattatcagt aatattcctt gtttgctact catatcagtt
     2221 cgtatacatt ttcataagtt tgtttttgaa aagaaaaagt ttaggaactt atgaagaaaa
     2281 gaagtatgct gtacttatat gcgcacgtaa tgaggaaaat gtaattggac agcttatatc
     2341 cagtatacat aatcaggatt actcaaaaaa tttgattgat atatatgtag cagctgataa
     2401 ctgcactgat gatactgcaa aaatagcaag gcagttaggt gcaatagtgt acgaaagaag
     2461 aaatacggaa aaagttggaa aaggttatgc tttaaatttt cttctttgca atattgattc
     2521 aaaatatgga attgaagaat atgccggatt ttttgttttt gatgctgaca atcttcttga
     2581 tgaatcatat attagtgaaa tgaataaggt tttttctaaa ggatttaata ttgtcactag
     2641 ttatcgcaat tccaaaaatt atgatactaa ctggatttct gcaggatatt ctttatggtt
     2701 tttaagagaa gcctgctctc taaatcaccc cagaatggca gctaaatcaa gttgtgcagt
     2761 atcagggact ggttttatgt tttcaaagga agttataaga aaatgcggag gttggaactt
     2821 ctacttactg acagaagata ttgagtttac ggtttataac ataatcaaca atgagaaaat
     2881 agggtattgc ccaacagcaa ttctttatga tgaacaaccg gtgacatttt cacagtcatg
     2941 gcaccagcgc atgcgatggg ctaaagggta tcttcaggta ttttcaaagt acggcttagc
     3001 tatgattaaa ggtattttca aaaaaacagg gttttcctgt ttcgacatgt gtatgtcaac
     3061 aatgcctgct gtgtttctta ccgcatttgg tattataatc aatatatcag caatttttgt
     3121 aggggtctta aatggtactg atgtaacagt agttttgggg tctatggttg aaactttctt
     3181 taatatgtat ttgatgcttt ttgcaattgg atttataact actgtaactc agtggaagat
     3241 gattcatgcg aattctttta aaaagatact atatctattc acatttccaa tattcatgat
     3301 gacatatata ccaatatcat taactgctat atttaagaaa gtggattgga aacctatcaa
     3361 acatactcac tgtgctacat tggctcaggt acgtatgaaa aaatgataaa aagaaaac
//
LOCUS       DAOSXM010000146         3265 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2111
            NODE_21236_length_3265_cov_12.781308, whole genome shotgun
            sequence.
ACCESSION   DAOSXM010000146 DAOSXM010000000
VERSION     DAOSXM010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864178
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3265)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3265)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:24:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,261
            CDSs (total)                      :: 2,229
            Genes (coding)                    :: 2,218
            CDSs (with protein)               :: 2,218
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 3 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3265
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21236_length_3265_cov_12.781308"
                     /isolate="AMR_MDS_2111"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(140..214)
                     /locus_tag="PLQ68_11030"
     tRNA            complement(140..214)
                     /locus_tag="PLQ68_11030"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(180..182),aa:Cys,seq:gca)
     gene            complement(224..298)
                     /locus_tag="PLQ68_11035"
     tRNA            complement(224..298)
                     /locus_tag="PLQ68_11035"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(264..266),aa:Gly,seq:gcc)
     gene            complement(302..377)
                     /locus_tag="PLQ68_11040"
     tRNA            complement(302..377)
                     /locus_tag="PLQ68_11040"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(342..344),aa:Phe,seq:gaa)
     gene            complement(381..457)
                     /locus_tag="PLQ68_11045"
     tRNA            complement(381..457)
                     /locus_tag="PLQ68_11045"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(421..423),aa:Asp,seq:gtc)
     gene            688..1794
                     /locus_tag="PLQ68_11050"
     CDS             688..1794
                     /locus_tag="PLQ68_11050"
                     /inference="COORDINATES: protein motif:HMM:NF039834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5711 family protein"
                     /protein_id="HXK73228.1"
                     /translation="MAKRRNKKAYWIIFVFLLVVAIAVGLIFFRTEILRFFKADVISK
                     DFGVESSIPISTGSIAASENGYIYIYADNGISVYKGNGEFLRSYLLSYSDTQITFSKT
                     HVIAYDKGATSYTVFRDGKKINEYNAKTPITYVFAFDEYMFLLCEGAAGYEGAVYCSS
                     YQTDVVIPDGYKNEIQYSVKYPIYIDIFDDEDKFVTVCCNPDDMSKTYIEVFEIGKST
                     PVSGITLEEFLPKIVCLEKGAFLAVNDNKMIKIDNKMSKTTILEDSIITYISRYDKNA
                     YGYAIIDDEVQIFKVDSENGIEWSKEVLSASSGLLSAEKKIIIWQGQSLSMFTLNGND
                     FHTIQADSQIQQVLAIGNKKLVIITPRNAVIYKY"
     gene            1807..2481
                     /locus_tag="PLQ68_11055"
     CDS             1807..2481
                     /locus_tag="PLQ68_11055"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HXK73229.1"
                     /translation="MNISDYILIGIILVSMIIGGVRGIIMSVYGLLTTILSAFLAFKF
                     AQPIRDLLVGTSFYINMHEKIENMVEALVPNLDSIEETLIATINSLPFPDDIKLWLIN
                     GIDFSQATSQAEAMGGLVVTVTDLIIAIMVGLVLFLILIIIFKLLKGLIKKICELPII
                     KQVDAIVGAVVGALSGVLLAYIICLIVSGFQASEVMNVIHENIQESKLASVLYNNNLL
                     LNMFKK"
     gene            2598..>3265
                     /locus_tag="PLQ68_11060"
     CDS             2598..>3265
                     /locus_tag="PLQ68_11060"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HXK73230.1"
                     /translation="MTNICIWGDSVMRGIVYDDDACKYIFLQNSCIPETAKNFDINYI
                     NNSKFGMTAPKALEKMKKTLKENDFKPDFALIELGGNDCDFDWNEVAENPDIDHQPNT
                     PMDEYEQSIIKMIETLREKGIEPVLTNLHPIDPVKYFDWITKDGLSKEAILKWLKDVF
                     KIYRFQELYSLTITKISQQLNVRLIDIRKAFLRAGNLMELLCKDGIHPNEKGHKIMTD
                     CLIS"
BASE COUNT         1130 a          466 c          642 g         1027 t
ORIGIN      
        1 gctgtttttg gttgaatgtt gaatgaaaaa gttgaattat taaagaatta actaataaaa
       61 ttttgaaaat ataataaaaa actcatttct ataattcaga gtatgtttct taattataga
      121 aatgagctta agtttaaagt ggaggcgcca tccggatttg aaccggagaa tgaaggtttt
      181 gcagacctct accttaccgc ttggttatgg cgccataaaa aaatggagcg ggttacgaga
      241 ttcggactcg cgactttcac cttggcaagg tgacactcta ccactgagtt aaacccgctc
      301 gtggtgccca gaggcggaat tgaaccgtcg acacagggat tttcagtccc ttgctctacc
      361 gactgagcta tctgggcata tggcgacctg gatggggctc gaacccacga cctccagcgt
      421 gacaggctgg cattctaacc agctgaacta ccaggccgca taattgggtg taaattgctc
      481 ttttaccaga tcttatacat aatattctgt ttaaaaagtc caattcacgt ccaaaatttg
      541 aaccgcttat tgagtataca aaatcggtat ttgtttgtca acagaaaaag ataccaaaaa
      601 gcaagattta gcagtataag ttgtttgtag agcgttatta gatatgatag aataagtcat
      661 gataaaaaga aaggcggata acgtcaattg gcaaaaagac gtaacaaaaa agcttattgg
      721 ataatatttg tattcctact tgtagtagcg atagctgttg gactgatttt tttcagaacc
      781 gagatactaa gattttttaa agcagacgta atatcaaagg attttggggt agaaagcagc
      841 attcccatat ctacaggaag tattgcagca agcgaaaatg gatatatata tatttatgcc
      901 gataatggca tttcagttta caagggtaat ggtgaatttc ttcgttctta tcttctttca
      961 tattctgaca ctcagataac tttttctaaa acgcatgtaa tcgcatatga taaaggagcg
     1021 acctcataca cagtatttcg tgatgggaag aaaattaatg agtataatgc taaaacaccc
     1081 attacttatg tgtttgcatt tgatgagtat atgtttcttt tatgtgaagg agcagccggt
     1141 tatgaaggtg ctgtatactg ttcatcttat cagacagatg ttgtaatccc ggatggatat
     1201 aagaatgaaa tacagtattc tgtaaagtat ccaatatata ttgatatatt tgatgatgag
     1261 gataagtttg taacagtgtg ctgtaatcct gatgatatgt caaaaacata tattgaagta
     1321 tttgaaattg gaaaatcaac accggtttcc ggtattactt tggaagagtt tttaccaaag
     1381 atagtctgct tagaaaaagg tgcatttcta gctgttaatg acaataaaat gataaaaatc
     1441 gataataaaa tgtctaaaac tactatttta gaggatagta ttatcacata catttcacga
     1501 tatgacaaaa atgcatacgg ttatgctata atagatgacg aagtacagat ttttaaagta
     1561 gattctgaga acggaatcga gtggagtaaa gaggtgttat ctgcttcttc aggtttgtta
     1621 tcagccgaga aaaagataat tatatggcag gggcagtcat tgagtatgtt tactcttaac
     1681 ggaaatgatt ttcatactat acaggcagat tcacagatac agcaggtttt agcgattggc
     1741 aacaaaaagc ttgttattat cacaccaaga aatgctgtca tctacaagta ttaaaggagc
     1801 agttaaatga atatttcaga ctatatctta atcgggatta ttttagtttc aatgattata
     1861 ggcggagtca gaggaataat aatgtcagta tatggattac ttactactat actaagtgct
     1921 tttttagcat ttaaatttgc tcagccaata agggatcttc ttgtcggaac cagcttctat
     1981 atcaatatgc atgaaaagat agagaatatg gtagaagctt tagtgcctaa tctggattca
     2041 attgaagaaa cattaatagc aacaataaat tcactgccat ttccagatga cataaagcta
     2101 tggttgatta atggtattga tttttcgcag gcgacatcac aggcagaagc tatgggaggt
     2161 cttgttgtta ctgtaacaga cttaattata gcgattatgg tgggtttggt attattcctg
     2221 attttaataa taatatttaa acttctaaag ggattaatta aaaagatttg cgaattacca
     2281 ataataaaac aagttgatgc aattgtaggt gcagtagtag gcgctctttc aggtgtactg
     2341 cttgcatata taatatgttt aatagtttcg ggatttcaag cttcagaagt tatgaatgtt
     2401 attcatgaga atatacagga aagtaaactt gcaagcgttt tgtataataa caacctgtta
     2461 ttgaacatgt ttaaaaaata aaataattaa aattaatttt ataaatattc aagaaaaata
     2521 agaatgcagc taaaagtcat atttggttca taatagcagt atatagttaa tgtattatta
     2581 tgtggaggat ttttattatg accaatatat gtatatgggg tgattcggtt atgaggggta
     2641 tcgtatatga cgacgatgcc tgtaaatata ttttccttca gaacagttgc atacctgaaa
     2701 ccgctaaaaa ttttgatatt aactatatca acaattcaaa atttggtatg actgccccaa
     2761 aagctcttga gaaaatgaaa aagacactta aggaaaatga ttttaaacct gattttgctt
     2821 taattgaatt aggaggtaat gactgtgact ttgattggaa tgaagtagca gaaaatcccg
     2881 atattgatca tcagcctaat acaccaatgg atgaatatga acaatctatt ataaagatga
     2941 ttgagacttt aagagaaaaa ggtattgaac ctgttttgac caatcttcat ccgatagatc
     3001 cggttaagta ttttgactgg ataactaaag acggattatc aaaagaagcc atacttaaat
     3061 ggttaaaaga tgtatttaag atatacaggt ttcaggagct gtattcgctt acaataacca
     3121 aaatatctca gcagttaaat gtacggctga tagatatacg aaaagctttt ttgagagcag
     3181 gtaaccttat ggagctttta tgcaaagatg gtatacatcc aaatgaaaaa gggcataaga
     3241 taatgacaga ctgccttata agcta
//
LOCUS       DAOSXM010000147         3257 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2111
            NODE_21321_length_3257_cov_10.186134, whole genome shotgun
            sequence.
ACCESSION   DAOSXM010000147 DAOSXM010000000
VERSION     DAOSXM010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864178
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3257)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3257)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:24:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,261
            CDSs (total)                      :: 2,229
            Genes (coding)                    :: 2,218
            CDSs (with protein)               :: 2,218
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 3 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3257
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21321_length_3257_cov_10.186134"
                     /isolate="AMR_MDS_2111"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(34..1182)
                     /locus_tag="PLQ68_11065"
     CDS             complement(34..1182)
                     /locus_tag="PLQ68_11065"
                     /inference="COORDINATES: protein motif:HMM:NF037527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK73231.1"
                     /translation="MNYEITNQFPNEIIYEEKGPFISLYQPTHRSYPDNKQDTIVFSN
                     LLRVIENSFNGNNKDIADSMLKPLYKLEKDLSFWNNTLDGIAVLASKNKCVIYNLQSP
                     VKEFVSLADSFHIKPLIMAFQSLDSYQVLGLSRNNFSIYQGNRYGFSEIELAPDVPRK
                     LEDVLGEDLTEPYLSHGSYGGTGGSAMYHGHADNKFEIDKDTEKYFRYVGKYVYENFS
                     KPSKLPLILISLKEYHSLFKDLSNNKYLISESIDYSYDSLTTDDIKTKVADIIDSINS
                     EKISGLVESFKNAEAESLASSDIINVSKAAFEGRIKTVLIEINKTMSGKINHSNGSVE
                     YQTEQDNSYSDLLDNIAQLVLLYKGEVFILPKDKMPSSTGISAIFRYI"
     gene            complement(1366..2265)
                     /locus_tag="PLQ68_11070"
     CDS             complement(1366..2265)
                     /locus_tag="PLQ68_11070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015943654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HXK73232.1"
                     /translation="MFDIPSYSSFKKIEAVNKGWSDDKKYYIETDDGRKLLLRLSDIS
                     KYERKKSDFEIMKKVASLNVPMSLPVDFGVCDNNTSVYQLLTWIDGEDAETVLPDLSD
                     IEQYNLGIQSGKALELIHSIPAPSSQEDWETRFTRKMNYKIKKYKECGIRFKGDELVI
                     EHIERNKYLLNNRPQSLQHGDYHIGNMIVLKDQKIHIIDWNRMDYGDPWEEFNRIVWS
                     ATVSPHFASGQLHAYFNGNPPDEFFKLLALYISSNTLSSIYWAIPFGQSEIDTMMKQS
                     QDILSWYDNMSSCIPSWYIKTSN"
     gene            2385..3167
                     /locus_tag="PLQ68_11075"
     CDS             2385..3167
                     /locus_tag="PLQ68_11075"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HXK73233.1"
                     /translation="MIDGHIHIERGDYTLEWIKKFTDKALEKNIEEIWLLEHCYRFTE
                     FVPMYDSVCQHSEYIDKWFQKKAGVKKLNDYLELMEKVRKTDFGVRIMFGIEVCYFKE
                     HEKFVYDLTKDKDFDFILGGVHFVDNFAFDHKAEHWEGKDVDLVYSQFFETSIDLIKS
                     NIFDGLAHPDSIKLFGHNTCYPLNEYYEELAKQLSNNNMYAEQNSGVHRRCPDTAELG
                     MNKDMLEAMMKHKVNIVTASDAHDPNDVGDKIAQLNKSILIG"
BASE COUNT         1001 a          524 c          581 g         1151 t
ORIGIN      
        1 cccttattat attcatcaat tgtaagggtt atgttaaata tatctgaaaa tcgccgaaat
       61 gcctgttgaa cttggcattt tgtcttttgg aagtatgaat acttcacctt tatataacag
      121 aaccagctga gctatattat ctagcagatc actgtaacta ttatcttgtt cagtctggta
      181 ctcaacagat ccgttagaat ggttgatttt acctgacatt gttttgttta tttcaataag
      241 cacagttttg attcttcctt caaaagcagc ttttgataca tttattatgt ctgaagatgc
      301 aagtgattcc gcttctgcat ttttgaagga ctcaactaaa ccagaaattt tttctgaatt
      361 aatagaatcg attatgtcag ctacttttgt tttaatatca tcggttgtca gagaatcata
      421 agaatagtct atgctttcac tgattagata tttattatta cttaagtctt taaataatga
      481 atggtactct tttagagaaa tcagtattag cggaagtttt gatggctttg aaaaattctc
      541 atatacatat ttacctacat atctgaaata cttttctgtg tccttgtcaa tttcaaactt
      601 gttgtctgca tgcccatgat acatagcgct acctcctgtt ccgccatatg acccatgtga
      661 caaatatggt tcggtcaggt cctcgcctag aacatcctca agctttcttg gtacatccgg
      721 tgctaattct atttctgaaa aaccatatct gttaccctgg tatattgaaa aattattgcg
      781 gcttaatcct aatacctgat aactatccaa tgactgaaaa gccataatca gcggcttgat
      841 atggaaactg tcagctaaag atacgaattc ctttacaggt gactgaaggt tatatattac
      901 acatttgttt ttactagcaa gcactgctat cccatcaagg gtgttgttcc aaaagcttaa
      961 atctttttca agtttgtata aaggtttaag cattgaatca gctatatcct tattattccc
     1021 attaaacgaa ttttctataa cgcgaagcag gttgctgaat actatagtgt cctgtttgtt
     1081 gtctgggtaa gatctatgtg ttggctgata aagagaaatg aagggtcctt tttcttcata
     1141 aataatttcg tttgggaatt ggtttgtaat ctcataattc ataactgtct cctttctgat
     1201 tcttcaatat gttattttta aagtgcagta aatcggaata tgaattccgt ttcaatgata
     1261 tttgttctaa agcttttttg cttttctttg tacccttatt atattctaca taggttttat
     1321 atgcaaatag tcaaagagta ttgatagagt caatttacat atgattcagt tggatgtttt
     1381 tatataccat gaaggtatac atgatgacat attgtcatac caggataata tgtcttgtga
     1441 ctgtttcatc attgtgtcta tttcgctttg tccgaatggt attgcccagt atattgatga
     1501 aagggtgtta ctggaaatgt atagtgctaa gagtttgaaa aactcatcag gcggattgcc
     1561 attaaaatat gcatgaagct gcccggatgc gaaatgagga ctgactgttg cactccatac
     1621 gattctgttg aactcctccc aggggtctcc gtaatccata cgattccagt cgataatatg
     1681 tattttctgg tcttttaata caatcatatt cccaatgtga tagtccccat gctgtaaaga
     1741 ctgaggacga ttgttaagaa gatacttatt cctctcaata tgttcaatta ccaattcgtc
     1801 tcctttaaac ctaataccgc attctttata cttttttatc ttgtagttca ttttgcgcgt
     1861 gaaacgtgtt tcccagtctt cctgtgaaga aggcgcaggt attgaatgta ttaactccaa
     1921 tgctttgcca gattgtattc ctaagttgta ttgctcaata tcagacaaat caggcaaaac
     1981 agtttctgca tcctcaccat caatccatgt tagaagctga tataccgatg tgttattatc
     2041 gcaaacaccg aaatctacag gtaaagacat gggaacattt aatgatgcta cttttttcat
     2101 tatttcaaaa tcactttttt tacgttcata tttggatata tctgataagc gaagaagcag
     2161 ttttctgcca tcatctgttt cgatgtagta tttcttatca tctgaccagc ctttatttac
     2221 agcttctatc tttttaaatg agctgtatga gggtatatca aacatgcttt tctccttatt
     2281 gcgaattaat cctattatat ccatttttgc attttattta aacaataagt atatattgtg
     2341 ataagatatg ttataacaaa aaaatacaga aagagaggat agttatgata gacggacata
     2401 tccatattga aagaggagat tatactcttg agtggattaa aaaatttaca gacaaagcat
     2461 tagaaaaaaa tattgaagaa atttggttac ttgaacattg ctacaggttt acagaatttg
     2521 tgcctatgta tgattctgta tgccaacaca gtgaatatat agataagtgg tttcagaaaa
     2581 aagcaggggt taaaaagtta aatgattatc ttgaactgat ggaaaaagtt agaaaaactg
     2641 atttcggtgt caggataatg tttggtatag aagtgtgcta ttttaaagag catgagaagt
     2701 ttgtatatga cttaacgaag gataaggatt ttgattttat acttggaggt gttcattttg
     2761 ttgacaactt tgcgtttgat cacaaagcgg aacactggga gggtaaagat gtggatttgg
     2821 tttattcaca attctttgaa acatccattg atcttattaa atcaaatatt tttgatggtc
     2881 ttgctcatcc tgattcaatt aaattgttcg ggcataacac atgttatcct ctaaatgaat
     2941 attatgagga acttgcaaag cagttatcca ataacaatat gtatgccgag caaaacagcg
     3001 gggtacacag aaggtgccct gatacagctg aattaggtat gaacaaggat atgcttgaag
     3061 ctatgatgaa acataaagtt aatatagtta cagcttctga tgctcacgac ccgaatgatg
     3121 ttggagataa gatagctcaa ttaaataaga gtatattaat tggttaaaat agatacttaa
     3181 gtattaaggt acttttggtg aataattaga aggtataaat ctgaccgaca tcctcataaa
     3241 gggtgtcttt caggtgc
//
LOCUS       DAOSXM010000148         3157 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2111
            NODE_22421_length_3157_cov_9.782721, whole genome shotgun sequence.
ACCESSION   DAOSXM010000148 DAOSXM010000000
VERSION     DAOSXM010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864178
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3157)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3157)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:24:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,261
            CDSs (total)                      :: 2,229
            Genes (coding)                    :: 2,218
            CDSs (with protein)               :: 2,218
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 3 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3157
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22421_length_3157_cov_9.782721"
                     /isolate="AMR_MDS_2111"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            122..>1828
                     /locus_tag="PLQ68_11080"
     CDS             122..>1828
                     /locus_tag="PLQ68_11080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK73234.1"
                     /translation="MEAVSWERIEGYIPSLRYKMQAEDTEVVIDCIATSLGDVLNISI
                     INNSNEYKNISLDACVLDNTVNTKWVDFDCEFSVVNPIFNDASDRIVIMDANITNNIP
                     RRREAVSFTYSLKPKESSKSKLIRPNKKYNEDIKELINTDWDFLCKEGLNTWKEIVKK
                     APVFSLPDSIVENAYKACLCDIFVMREEQADGRIAGLAGTEMYRSANTGEPCFQSLAL
                     GKYGFFEQAKENIEFVAQFQIENGSWEDDKQWGKYMWATSGWKSHSIKQYYLRTRDKK
                     FLEENFERMLRSAKWSKEQRDKIKQKKNPEDPEWGLMPRGMGDCGLKDNDDLFGVFYP
                     HNFLHCMGIEIAAWSAKELGRDDEYARLNSDYLDYKECIIKSLDKAHIREDDKSIWIG
                     SSPNNKSGSRWGVADVVYPSNILPVNHPLAVGTMKKLQSNISQGGLPKNLGWIPDGLW
                     VAISLDAMAYVNILAGNADLSANYLIAAINHGTPLYTWCEERTEEKGAIKITGDLQHA
                     WTPICITQFVRDMLISDHLFNDDILHITPAVPRYFYEDGNTIEIKDAPTYWGNISFTI
                     KRN"
     assembly_gap    1829..1928
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1929..2377)
                     /locus_tag="PLQ68_11085"
     CDS             complement(<1929..2377)
                     /locus_tag="PLQ68_11085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HXK73235.1"
                     /translation="MNKTFGCIFDIQRFSLHDGPGIRTTVFFKGCNLRCFWCHNPESI
                     NLHPEIQIFKHKCIHCGKCISVCPQNAIHKNGNSYLFSRDLCIKCGKCVDICYAGARV
                     IAGRTVSAFDVMAEIMEDALFYQDNSGGVTFSGGEPMLQPVFLKALLV"
     gene            complement(2370..>3157)
                     /locus_tag="PLQ68_11090"
     CDS             complement(2370..>3157)
                     /locus_tag="PLQ68_11090"
                     /inference="COORDINATES: protein motif:HMM:NF014900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pyruvate formate lyase family protein"
                     /protein_id="HXK73236.1"
                     /translation="HGPIEKGLDASNGGLEYINLCIDGAGLAVVADSFAALKERIEDK
                     SQLNWQQIHEAIINNYEGYQEILLMMKNSSHYGYGGTTADQFAVKIVNEFVKIVKKSP
                     TPNGHNMIPGLFSWANTIPMGKAVGATANGRLAYTPISHGANPLPGFKDSGALTAMAK
                     AVASVQCGYGNTVPIQLEINPITIKGENGINNMMNFISVYLNKMGGTLMNINILDKEK
                     ILDAHSNPSKYPDLIVRVTGFCAYFALLSEDFRKLVVDRIINE"
BASE COUNT         1030 a          548 c          557 g          922 t
ORIGIN      
        1 gatagttgct tttgtgatat attgttcctc cgttctttta tagtacaacc ctttaggtgc
       61 atacaaaagg gtacggattg actggaaagt caaaattact gctaaatgca atgatactcc
      121 tatggaggca gtatcatggg aaagaattga aggatatatt ccatcattaa gatataagat
      181 gcaagctgaa gatacggaag tagttatcga ttgtattgca acatctttgg gtgatgttct
      241 taatatttca ataatcaata attctaatga atataagaat attagcctgg atgcttgtgt
      301 tttagacaac acagtaaata ctaaatgggt tgattttgac tgcgaattta gtgtagtaaa
      361 cccaatattt aatgatgcat cagacagaat agtcattatg gacgcaaaca tcacaaataa
      421 catacctaga agaagagaag cggtctcttt cacttactcc ctaaaaccta aagaatcatc
      481 taaaagcaag ctgattcgtc ctaacaagaa atacaatgag gatataaaag aactaataaa
      541 tactgattgg gacttcttat gtaaagaagg cttaaacaca tggaaagaaa tagtaaaaaa
      601 agcaccagtt ttcagtcttc cggactcaat agtagaaaat gcatataagg catgcttatg
      661 tgatatattc gtaatgagag aagaacaggc agatggcaga attgcaggac ttgctggaac
      721 tgaaatgtat agaagcgcta ataccggcga accctgtttt caatctctgg ctttaggaaa
      781 gtatggtttt tttgagcagg caaaagaaaa tatagagttt gtcgcacagt ttcagattga
      841 aaacggctca tgggaagacg ataagcaatg gggtaaatat atgtgggcta cctcaggatg
      901 gaaaagccac agcataaaac aatattatct aagaaccaga gacaaaaaat ttctggaaga
      961 aaactttgaa agaatgcttc gttctgcaaa atggtccaaa gaacaaagag ataagataaa
     1021 gcagaagaaa aatcctgaag accctgagtg gggacttatg ccaagaggca tgggagactg
     1081 cggattaaag gataatgatg atcttttcgg tgtgttttac cctcataact tccttcactg
     1141 tatgggtatt gaaatagcag catggtctgc taaagaatta ggcagagatg atgagtatgc
     1201 taggcttaat tctgattatc tagattacaa ggaatgcatt attaaatctt tagataaagc
     1261 tcatataaga gaagatgata aaagcatatg gataggcagt tctccaaaca ataagtctgg
     1321 tagcagatgg ggtgtagctg atgtcgttta tccttcaaat atccttccag taaatcaccc
     1381 attagctgtt ggaacaatga aaaagcttca gagtaatata agtcaaggag gtctgcctaa
     1441 gaatctaggc tggatacctg atggcttatg ggtagcaatt tctcttgatg ctatggcata
     1501 tgttaacatt ttagcaggta acgctgattt atcagcaaac tatctaattg ctgctataaa
     1561 ccatggaacc cctttataca catggtgtga ggaaagaact gaggaaaaag gagctataaa
     1621 aattacaggt gacctgcaac atgcatggac tcctatatgt attacgcaat ttgtaagaga
     1681 catgctcata tccgatcatc tttttaatga cgatattcta catataacac ctgcagttcc
     1741 gagatatttc tatgaagatg gtaatactat agaaataaaa gatgctccca catattgggg
     1801 caatatatct tttacaatta aaagaaacnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnac aagcaaggct tttaaaaaaa caggttgcaa cataggttcc ccgcctgaaa
     1981 aggttacacc tccgctgtta tcttgataaa acagtgcatc ttccataatt tcagccatta
     2041 catcaaatgc actcactgtt cttcctgcaa tcactcttgc tcctgcatag catatatcaa
     2101 cacatttacc gcattttatg cataaatccc ttgaaaataa ataagaatta ccatttttat
     2161 gtattgcatt ctgaggacat actgatatgc atttgccaca atgtatgcat ttatgcttaa
     2221 aaatttgaat ttccggatgc agattaattg actctggatt atgacaccag aaacatctca
     2281 aattacagcc tttgaaaaaa actgtagtcc taatgcccgg gccgtcatgc aaagaaaatc
     2341 tctgtatgtc aaatatgcat ccaaatgttt tattcattga ttatcctgtc tactacaagc
     2401 tttctgaaat cctcagataa tagagcaaaa tatgcgcaaa aacctgtaac tctgacaata
     2461 agatctggat attttgaagg attactatgc gcatctaaaa tcttttcttt atcaagtata
     2521 ttaatattca tcaatgtacc acccatttta tttaaatata ccgatataaa attcatcatg
     2581 ttatttatgc cgttttcacc ttttattgta attggattaa tttccaattg tattggtaca
     2641 gtatttccat atccgcattg cacagatgca acagcttttg ccatagcagt caacgctccg
     2701 ctgtctttaa aaccaggaag aggattcgcg ccgtgtgata taggcgtata cgcaagccgt
     2761 ccattagcag tcgcacccac tgctttaccc attggaattg tattagccca tgaaaaaaga
     2821 cctggtatca tgttgtgtcc attaggagtt ggagacttct taactatttt tacaaattca
     2881 tttacaattt ttactgcaaa ttgatctgct gttgttcctc catatccata atgagatgaa
     2941 tttttcatca taagaagaat ttcttgataa ccttcatagt tatttattat ggcttcatgt
     3001 atctgttgcc agttaagttg tgatttatct tcaatacgct cttttaatgc tgcaaaactg
     3061 tctgctacta ctgctaagcc tgctccatca atgcaaagat tgatatattc caacccccca
     3121 ttagaagcat ccaaaccctt ctcaattgga ccatgac
//
LOCUS       DAOSXM010000149         3121 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2111
            NODE_22830_length_3121_cov_10.243640, whole genome shotgun
            sequence.
ACCESSION   DAOSXM010000149 DAOSXM010000000
VERSION     DAOSXM010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864178
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3121)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3121)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:24:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,261
            CDSs (total)                      :: 2,229
            Genes (coding)                    :: 2,218
            CDSs (with protein)               :: 2,218
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 3 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3121
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22830_length_3121_cov_10.243640"
                     /isolate="AMR_MDS_2111"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(156..959)
                     /locus_tag="PLQ68_11095"
     CDS             complement(156..959)
                     /locus_tag="PLQ68_11095"
                     /inference="COORDINATES: protein motif:HMM:NF024793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HXK73237.1"
                     /translation="MYKQFYGMSSNPFDKNLDTKNAYMTQDMRAIQGRLTHLKEHPGI
                     GVFTAGAGQGKTFALRYFAEQLNPNITKFYYICLSTVTTSEFYRQLCNMLGLETSYNK
                     SSMFRAIQEYFENMSNNKKIHCMVCLDEAQYLNADILRDLKMLCNFAMDSKNCFSLLL
                     LGQPTLINILMRQPNEALRQRITVNYQFEGLQEKETIAYIKKQLELVGTNSGVIDESA
                     MLSAYGSCGGSLRKLNLILTKALMIGAQNRKQNIDTDIILSAVNDMELC"
     gene            complement(952..2202)
                     /locus_tag="PLQ68_11100"
     CDS             complement(952..2202)
                     /locus_tag="PLQ68_11100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016289881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HXK73238.1"
                     /translation="MDQKTMIEIASIRLALIQPAFNGTYPDATKKQYYERISQIPLKL
                     PGGKEVKYAYGTLACWESAYRKKGFDALLPKERSDLGSSRKLDDDTIAAILRIRCEFP
                     KINATEIYHKLISDGMVGKKEVSLTTVQRYIKKHNLKGASAPGQKDRKAFEEEFACGM
                     YQGDTLYGPYLVEDGLRRRTYLIMLLDDKSRMIVSGRFFYADNAYNFQKVLKDAVASR
                     GIPTKLYVDNGSPYKNEQLAMICGQLGTVLIHTPVRDGASKGKVERNFRTLRSRFLNV
                     LDTSKLSGIDELNSELLAYINKHNTTVHSATGETPQERYMRDMANIKMAFDRELLDQC
                     FMNRIKRLVRNDATVMIDKEMYDVPMEFIRTKVEIRYLPDAMEKAYILYENKQYLIRK
                     TNKVENGKTKRNNSYELNYGGSNV"
     gene            complement(2244..2894)
                     /locus_tag="PLQ68_11105"
     CDS             complement(2244..2894)
                     /locus_tag="PLQ68_11105"
                     /inference="COORDINATES: protein motif:HMM:NF040103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6431 domain-containing protein"
                     /protein_id="HXK73239.1"
                     /translation="MSRTTTIMIRIFLNFCKCFSKIHKLNEYQMLQMLEDSIYDKLLT
                     CPTCGAPSTAFHKDGDYERDFICYEEGTPVFHKIVIHCIECNSCGHSHALEPSVIVPY
                     SSYSIGFLLQVIHAKIVGRFSTVLELCEHFQIAIATYYRILKKYMLDSLLLEKLLEDM
                     VAMIQPEHYHLSSFHQCLREFFLINGYSFFQPCVRLCKNMKMQKLLSPSSRYIENG"
BASE COUNT          935 a          581 c          494 g         1111 t
ORIGIN      
        1 agtgtaaaac agcactttac cgctaactta taaagcattt tatttctatt gtttttgaat
       61 tcataaaagc acattacacc tacgactgca tgatatttat atagaaatgc aaattaatcc
      121 aaatagaaaa gagaccctat taaggtctct catatttaac ataattccat atcatttaca
      181 gcagataaaa ttatatcggt atctatattc tgttttctgt tttgtgcacc aatcataagg
      241 gcttttgtta aaatcaggtt gagttttcta agagaacctc cacaacttcc ataagctgat
      301 agcattgcac tttcatctat taccccactg tttgttccca ctaattccaa ctgcttcttt
      361 atatatgcta tggtttcctt ttcctgcaat ccttcaaatt gataatttac tgtaattctc
      421 tgtcgcaaag cttcatttgg ctgtctcatt aagatattaa tcagtgttgg ttgtcccaat
      481 aataaaagag aaaaacagtt ttttgaatcc atagcaaagt tacatagcat ttttaaatct
      541 cttaaaatat ctgcattgag atactgtgct tcatctagac ataccataca atgtatcttc
      601 ttgttattgc tcatattttc aaagtattcc tgtattgctc gaaacataga ggatttatta
      661 tacgatgttt caagtcctag catgttacaa agctgtctat agaattcact cgttgtaacg
      721 gtagataaac agatataata aaattttgta atgtttggat ttaattgttc tgcaaagtaa
      781 cgaagtgcaa aggtttttcc ctgacctgct ccagctgtaa aaacaccaat tccggggtgt
      841 tccttaaggt gggtcagtct tccttggatt gccctcatat cctgtgtcat atatgcattt
      901 tttgtatcca gattcttatc aaagggatta ctactcattc cataaaactg tttatacatt
      961 gctacctcca taattcaact catagctatt gtttctcttt gtttttccgt tttctacttt
     1021 atttgttttc cgaattaaat attgcttgtt ttcatataga atatatgcct tctccatagc
     1081 atctggtaga tatcgaatct ctacttttgt tcgaataaat tccataggga catcatacat
     1141 ctctttgtct atcattaccg tagcatcatt acgaaccaat ctttttattc ggttcataaa
     1201 acattgatct agtaattctc tatcaaatgc catttttata tttgccatat ccctcatata
     1261 acgttcttgt ggtgtttctc ctgtagcaga atgtaccgtt gtattatgtt tgtttatgta
     1321 cgcaagtagt tcactattta attcatcgat tccagaaagc ttagaggtat ctagtacatt
     1381 tagaaaccta cttctaaggg ttctgaaatt tctctctacc ttgcctttgc tagcaccgtc
     1441 tcttactggg gtatgaataa gtaccgttcc taactgccca cagatcatgg caagctgttc
     1501 atttttataa ggggaaccat tatctacata tagttttgtg gggattcctc tggatgcgac
     1561 tgcatctttt agtacttttt gaaagttata cgcattatcc gcataaaaga accgtccact
     1621 tacaatcata cgtgacttat catctaatag cataattaga taggttcttc ttcgtagccc
     1681 atcttctaca agatatggtc catatagcgt atctccctga tacataccac aagcaaattc
     1741 ctcttcaaat gctttccgat ctttttgacc tggagcacta gcgcccttta gattatgctt
     1801 ttttatatat cgttgcactg ttgtaaggga aacctccttt tttcctacca taccatctga
     1861 aatgagcttg tgatatattt ctgttgcatt tatctttgga aattcacatc gtattcgcaa
     1921 aatagctgca atagtatcat catcaagctt tcgactactt cctaaatcag aacgttcttt
     1981 agggagaagt gcatcaaatc cttttttccg ataagcagac tcccaacaag caagtgttcc
     2041 ataggcatat tttacttcct ttcctcctgg aagttttaat ggtatctgcg aaatcctttc
     2101 gtaatactgt ttttttgttg cgtcaggata cgtaccatta aatgctggct gaataagagc
     2161 cagccgtata ctagctattt ctatcattgt cttttgatcc attaaaatca cctccaaggt
     2221 cagcatagca tttttatatc tgcttatcca ttttcaatgt atcggctgga aggtgataag
     2281 agtttttgca ttttcatatt tttgcataat ctgacgcagg gttgaaaaaa agagtatcca
     2341 ttaattagaa aaaactctct taggcattgg tgaaaactgg aaagatgata atgctctggt
     2401 tgtatcatag caaccatatc ttctaggagc ttctctaata aaagggaatc cagcatatat
     2461 ttttttagta ttctatagta ggtagcgatt gcgatttgaa aatgttcaca taattctaaa
     2521 accgtagaaa aacggccaac gattttcgca tgaattacct gtaataaaaa tcctatacta
     2581 taggatgaat aaggcactat aacagatggt tctaaagcat gagaatgtcc acaagaatta
     2641 cattcgatac aatggataac aatcttatga aacacagggg taccttcctc atagcagatg
     2701 aaatctcttt cataatctcc atctttgtga aaagcagtag aaggagcacc gcaagttgga
     2761 caggttaata gcttatcata aatagaatct tctagcattt gtagcatttg atattcattt
     2821 aatttatgta ttttagaaaa acacttgcaa aagttcaaaa atattcttat cataatagtt
     2881 gttgttctag acatcattgt ctgggataaa tgtggatgaa ggacctagtg cttgccggcg
     2941 cgatgagggt ccttcttttt ttggttactc taagataata aaaaaagacc tcggtaaggt
     3001 caataggaat agaaatattt tgcagttata aaaagcaatt tattactaga tataatagca
     3061 cgactagcaa taatctgatg ttagaagtgt gtttgagata ttaataattt ggggtattac
     3121 a
//
LOCUS       DAOSXM010000150         3077 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2111
            NODE_23329_length_3077_cov_9.049636, whole genome shotgun sequence.
ACCESSION   DAOSXM010000150 DAOSXM010000000
VERSION     DAOSXM010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864178
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3077)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3077)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:24:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,261
            CDSs (total)                      :: 2,229
            Genes (coding)                    :: 2,218
            CDSs (with protein)               :: 2,218
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 3 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3077
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23329_length_3077_cov_9.049636"
                     /isolate="AMR_MDS_2111"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(312..1619)
                     /locus_tag="PLQ68_11110"
     CDS             complement(312..1619)
                     /locus_tag="PLQ68_11110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK73240.1"
                     /translation="MKKLLVVLIALCAATAVFAEDAAKFNFNANGFAVATNYAGVEKD
                     SGGDFGEARFQPLFNLTTGSFDFVLKLRIDTEFGANDDSANNDDFGSSSQFSSGTKQK
                     AIKVLNAYGKSKVDAMEGLSLAAGVMSYDYPLVYSDNMPMFTAAYELNPITLNLYYGK
                     LSEGDIDKGKDDSQVYIADAVIKFGESSIRPAFFYHQVRVNSYDNTTSNLQDLLGTTD
                     PIKGSGYIYAINASIVAGQFGIDATGVYESAKYKDTATSEDVKIGAYAVDVAPYIKVG
                     EAVKLTAFMTMVSGWDGSGSKDKSFLDGSLDPSSGLNTLRLYIIEDCGTFGSNSAFTG
                     GSNMRYSDNSGYSAYGLAFDGSFGPVTMKVQGAYAKAAKVASGVKKDLGIEADVNIGY
                     ALTKVSTIFVEGAYLKTGKYFENGGSVDTQNVNYIAAGMTFSI"
     gene            complement(1816..>3077)
                     /locus_tag="PLQ68_11115"
     CDS             complement(1816..>3077)
                     /locus_tag="PLQ68_11115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK73241.1"
                     /translation="LLFAGSAFAQDAAPKGPDFKYGVWANAFSISEKTAVDESDKSYS
                     QIRVRPMFTMGIENVSVVTRFEIDQYFGKGGEGATSSSTNTFGNGSSNSSTYADSDTD
                     QIAVEVKWAYINVKDFFIPGLTATAGLAPFVYSVGYNNDMPLFNLVYDAGVVKVDLSY
                     VKVEENDLDNTDEIGLNDDNDDIQMYAAKLPLSFGDVVITPAVLYTMSEGQNTAGEYT
                     TTAPSIAVKVKAGAFSFDGDFVYITGKMEDADLDVKAYAGYANAGFKASDMFSINIFG
                     LYATGESDDTDEINSFQTACGDEIEVGPLFIINDGGNIGQVGVSNEYDKATEGLMAYG
                     LAFNFKVDKFAALAQVAYATTTSDDIVDDKAIGTEFDLRLSYEVGPKTSFWVEGAYLK
                     AGKYIETKNGGKCDDPSYYAAGIMTSL"
BASE COUNT         1009 a          641 c          493 g          934 t
ORIGIN      
        1 tgaatttaag attcgaacag gtgctcgctg acatgaacag cagatttgag caggtggata
       61 agaggttcag catgatgatg tggtttacag gtatcggatt tacactggtt accacaatta
      121 ttacaggggc ttttgttctg ctgaaatgat attgtttttt ctctcgcaaa ggcgctaagt
      181 cgcaaagaag atacaattgt tttaacacag atggacacag agaaaggaaa aaattttcga
      241 tgtatataaa acagaaagag aaccttgcgg ttccctttct gttttataat ttttttaaac
      301 tacttactga attaaataga aaatgtcata ccagctgcaa tataatttac attctgtgta
      361 tcaacagaac caccattttc aaaatattta ccggttttaa gataagcgcc ttcaacaaag
      421 atagttgata cttttgttaa agcatagccg atatttacat ctgcttcaat accgagatct
      481 tttttaacac ccgaagcaac tttagcagct tttgcataag caccctgcac tttcattgtt
      541 acaggcccaa aacttccatc aaatgcaaga ccatatgctg aataaccgga gttatcacta
      601 tacctcatat ttgatccacc tgtaaatgct gaattacttc caaaagttcc acaatcttca
      661 attatgtata atcttaacgt attaaggcca ctacttgggt caagagatcc gtcaagaaat
      721 gatttatctt tagaaccaga tccgtcccag cctgaaacca tagtcatgaa agctgttaac
      781 ttaaccgctt caccaacttt gatatatggc gctacatcaa ctgcataagc accaatttta
      841 acatcttcag atgtagcagt atctttatac ttagcagatt cgtaaacacc agttgcgtca
      901 ataccaaatt gacctgcaac aatgcttgcg ttaatcgcat agatataacc tgaacccttt
      961 atcggatcag ttgtaccaag aagatcctga agattcgaag ttgtgttgtc ataggaatta
     1021 actcttactt gatgatagaa aaaagccggt cttattgaag attcaccaaa tttaattacc
     1081 gcatctgcaa tataaacctg agaatcatct ttccctttat caatatcacc ttcagaaagc
     1141 tttccataat aaagattaag agttatggga ttgagttcat atgcagcagt aaacataggc
     1201 atattatcac tatagacaag cgggtagtca taactcataa caccagccgc cagtgacagt
     1261 ccctccatag catcaacctt gctttttcca taagcattaa gaactttaat agctttctgt
     1321 tttgtaccgc tgctaaactg ggaagagctt ccaaaatcat cattgttagc gctgtcatca
     1381 ttagcaccaa attctgtatc aatcctgagt ttaaggacaa aatcaaaaga acccgttgtt
     1441 aaattaaaaa gaggctgaaa tcttgcctca ccaaaatcac cacctgaatc tttttcaaca
     1501 ccggcataat ttgttgcaac agcaaagccg tttgcattga aattaaactt agccgcatct
     1561 tctgcgaaaa ctgcagttgc agcgcagagt gcaataagaa ctactaataa cttcttcatt
     1621 gatctctcct aaatctttaa tttttattag aaatagtaat aaatattaca ttactatcat
     1681 tttaaaatct tataaaactc tttaaagcga ttgtcaagaa gatattaaat agaggaagtt
     1741 acaaaaatat taaataaagc aaaaaaaaat cggggcatct gccccgattt ttctcctgaa
     1801 ttaaattaca gaatcttaga gacttgtcat tataccagct gcatagtatg atggatcatc
     1861 acatttgccg ccattttttg tttcaatata ttttccagct ttgaggtatg caccctcaac
     1921 ccagaaagat gttttaggac caacttcata agaaagtctg aggtcaaatt ctgtaccgat
     1981 cgctttatca tcaactatat catcagatgt tgttgtagca taagcaacct gtgcaagtgc
     2041 agcaaattta tcaactttaa aattaaaagc aagaccataa gccatgagac cttctgtagc
     2101 tttgtcatac tcattagaaa caccaacctg accgatattt ccaccatcat tgattataaa
     2161 cagcgggcca acttcaatct catcaccaca agcagtctgg aatgaattaa tttcatcagt
     2221 gtcatcgctt tcgcctgttg catagagacc gaagatgttt atagaaaaca tatcagaagc
     2281 tttaaatcct gcatttgcat atccagcata agctttaaca tctaggtctg catcttccat
     2341 cttgccggta atatatacga aatcaccatc aaatgaaaat gcacctgctt taactttaac
     2401 cgcaattgaa ggagcagttg ttgtatattc acctgctgta ttctgtcctt ctgacattgt
     2461 ataaagaaca gctggagtta taacgacatc accaaaagaa agcggaagtt ttgctgcata
     2521 catctggatg tcatcattat catcatttaa gccaatttca tcggtgttat caagatcatt
     2581 ttcttctact ttcacatatg aaagatcaac ttttaccaca cctgcatcat acaccaggtt
     2641 aaaaagcggc atgtcattat tataacctac agagtaaaca aaaggtgcaa gacctgctgt
     2701 agctgtaagt ccggggataa agaaatcttt aacattgata tacgcccatt tcacttcaac
     2761 agcaatctgg tctgtatcag agtctgcata agttgatgag tttgatgatc catttccaaa
     2821 agtattagtt gatgaagatg tagcaccctc accacctttt ccaaaatact ggtcaatttc
     2881 aaatcttgtt acaacagaaa cattctcaat ccccattgta aacattggtc ttacccttat
     2941 ttgagaataa gacttatcac tttcatctac agccgttttt tcagaaatag aaaaagcgtt
     3001 tgcccataca ccatatttga aatccggtcc cttcggtgct gcatcctgtg cgaatgcgct
     3061 gcctgcgaag agaagtg
//
LOCUS       DAOSXM010000151         3046 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2111
            NODE_23692_length_3046_cov_7.851555, whole genome shotgun sequence.
ACCESSION   DAOSXM010000151 DAOSXM010000000
VERSION     DAOSXM010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864178
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:24:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,261
            CDSs (total)                      :: 2,229
            Genes (coding)                    :: 2,218
            CDSs (with protein)               :: 2,218
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 3 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3046
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23692_length_3046_cov_7.851555"
                     /isolate="AMR_MDS_2111"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(17..1894)
                     /locus_tag="PLQ68_11120"
     CDS             complement(17..1894)
                     /locus_tag="PLQ68_11120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013658911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4914 family protein"
                     /protein_id="HXK73242.1"
                     /translation="MKEIINDIKLPDYMADIIRNCKSVYSPESREDLLALAFGKNKEG
                     IFNVEYEVEGLGIIKEAYVTRCKNGAAVNYPEDYMRRRDPDCLLVSDDNETDKPRYKD
                     AYKNNFDDLRELTFNWLKKQDLIFLPFKAGGDEYGYDAVLLAPANAAFFACGLADLQG
                     FISISKVEKTFCPKAAIFLAPPFRHTHFGGKQIVVHNRLEKIHELFSYNLYPGPSAKK
                     GIYGVLLNIGEKEGWITAHASAVKVVTSYDNEIVIMHEGASGGGKSEMIENIHKEMDG
                     RALVGQNIITGEKNYINLNETCELRPITDDMALCHPKMQNDSKKLVIKDAEQGWFLRL
                     DHIKGYGTTPQYEKIFTQPSEPLIFLNIDAVADSTCLVWDHIIDSNGKPCPNPRVILP
                     RRLVSNVISDPVEVDVRSFGVRTPYCTKDNPSYGIIGLFHVLPPALAWLWRLVAPRGH
                     NNPSITDSAGMSSEGVGSYWPFATGKMVEQANLLLDQIISSENTRYILIPNQHIGAYK
                     VGFMPQWITREYIARRGSAKFKPEQLVRSRCSLLGYSLDSLKVDGQYIRKVLLQPHLQ
                     VEVGYEGYDEGSRILTDFFKKEILKFDTPSLKPLGHEIIKLLLNDATVDEYQSLITMK
                     H"
     gene            complement(1952..2455)
                     /gene="purE"
                     /locus_tag="PLQ68_11125"
     CDS             complement(1952..2455)
                     /gene="purE"
                     /locus_tag="PLQ68_11125"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013658912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HXK73243.1"
                     /translation="MSKKVAIIMGSDSDLPVVKGAITVLKNIGIPIEVHVISAHRTPL
                     IASEFAIKAKEDNFGVIIAAAGKSAHLAGVLAAYTTIPVIGIPCKSSALEGLDALLST
                     VQMPKGVPVATVAIDGAENAGILAAQILGVSDNLISDKLKKMKIDMEDDVRKKDEKLQ
                     NEIWWKL"
     gene            complement(2562..2927)
                     /locus_tag="PLQ68_11130"
     CDS             complement(2562..2927)
                     /locus_tag="PLQ68_11130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013658913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2294 domain-containing protein"
                     /protein_id="HXK73244.1"
                     /translation="MTKGQLEAKISEVVSRFEIEYMGRGPKLIRTHILNDMIIVRLTG
                     FLSPSEQKLTNNPQGIELFKKVRTSLFEGGRGYLETLITEVIDVAIVSTHSDISTKTG
                     EKVIIIITDRNLEELVTKK"
BASE COUNT          902 a          622 c          405 g         1117 t
ORIGIN      
        1 ctcttaatac aaacgtctag tgcttcattg ttataaggct ttgatattcg tctacagtag
       61 catcatttag tagtagttta attatctcat ggccaagagg ttttaatgaa ggagtatcaa
      121 attttaaaat ttctttttta aaaaagtctg ttaatattct tgaaccttca tcatatcctt
      181 catatccaac ctcaacttgc aaatgaggtt gcaaaagaac ctttctgata tattgtccat
      241 ctacttttag tgaatctaat gaatatccca gtaaagaaca tctagaacgt actaattgtt
      301 ctggcttgaa tttggcacta ccccttctag caatatattc tcttgttatc cattgtggca
      361 taaatccaac cttatatgct cctatatgct gattgggaat tagaatgtat cttgtgtttt
      421 ctgaactaat aatttgatca agcaatagat ttgcttgttc aaccatttta cctgttgcaa
      481 aaggccaata tgatcccaca ccctcgctac tcattcctgc agaatcagta atactcgggt
      541 tattgtgccc tcttggtgca acaagtctcc ataaccatgc taaagctgga ggcagtacat
      601 gaaataaccc tataatacca tatgatggat tatcttttgt acaataaggg gttctcactc
      661 caaaactacg tacatcaact tcaactggat cactaattac atttgaaact agcctacgag
      721 gtaaaattac cctcggattt ggacatggtt ttccattaga atcaataata tggtcccaga
      781 caagacatgt tgaatcagca acagcatcaa tatttaaaaa aatcaaaggt tctgatggtt
      841 gtgtaaatat tttctcatat tgaggagttg ttccatatcc ttttatgtgg tctagtctta
      901 aaaaccatcc ctgctctgca tctttaataa ccagtttttt actatcattc tgcatttttg
      961 gatgacaaag cgccatatcg tcagtaatag gtcttaactc acatgtttca tttaaattta
     1021 tatagttttt ttcacctgta atgatatttt gaccaaccag ggctctacca tccatttctt
     1081 tatgaatatt ttctatcatt tcactcttac caccacctga agctccttca tgcataatca
     1141 caatctcgtt atcatatgaa gtaactactt ttactgcaga agcatgagca gtaatccatc
     1201 cctctttttc cccaatgttt aaaagtacac cataaatacc tttttttgca ctaggacctg
     1261 gataaagatt atatgaaaat agttcatgta tcttttctaa tctgttatgg acgacaatct
     1321 gcttgcctcc aaaatgagta tgcctgaatg gaggtgcaag aaaaattgct gccttgggac
     1381 aaaatgtttt ttctacttta ctgatactta taaacccttg taaatctgca agtccacaag
     1441 caaaaaaagc agcattagct ggtgcaagaa gtacagcatc ataaccatat tcatcacccc
     1501 ctgctttaaa aggaagaaaa atcaaatcct gttttttcaa ccaattaaaa gttaattccc
     1561 ttaaatcatc aaaattattt ttataagcat ctttatatct aggtttatca gtttcattat
     1621 catcagaaac caacaaacaa tccgggtcac gtcttcgcat atagtcttca ggataattta
     1681 ccgcagcacc atttttgcat cttgtcacat atgcttcttt tattatacct aagccctcaa
     1741 cttcatactc aacattgaaa atgccttctt tattttttcc aaatgctaaa gctaacaaat
     1801 cttctctcga ttcaggacta tacactgatt tacagtttct tattatatca gccatataat
     1861 ctggtagttt gatatcatta attatttctt tcatataaaa cctcttcttt ctttatttaa
     1921 atttcttttg tttgatttat ccataattcg ctcaaagttt ccaccatatt tcattttgta
     1981 atttttcatc tttttttctt acatcatctt ccatatctat cttcattttc ttcagcttat
     2041 cagatattaa attatctgac actcctagta tctgtgctgc aagaatgcca gcattttctg
     2101 ctccatcaat agctacagta gctactggta caccttttgg catctgaact gttgataaaa
     2161 gcgcatctag cccttcaagt gccgaagatt tacatggtat acctattact ggtattgttg
     2221 tatacgctgc taaaactcca gctaaatgtg cagattttcc tgctgctgca ataattaccc
     2281 cgaaattatc ttcttttgct tttattgcaa actcacttgc aattaaaggt gtacggtgtg
     2341 cactgataac atgtacttct attggaatac caatgttttt caatactgta attgctcctt
     2401 tcacgacagg tagatcacta tcgcttccca ttatgattgc tactttttta ctcataactg
     2461 cctcctaatc tagttataca aaaaagccga taacctttaa tattcttaag gtatcggctt
     2521 acttacaact tagctatgct attttgtgtt gtcttccggt tttacttttt cgttactaat
     2581 tcttctaaat ttctgtccgt tataataatt attacttttt ccccagtttt tgtacttata
     2641 tcagagtgag tgctgactat agctacatca ataacttcag taatcaatgt ttccaaatat
     2701 cctctcccac cttcaaaaag ggaagttcgt acttttttaa acaactcaat cccttgggga
     2761 ttattagtta gtttctgttc tgaaggactt agaaatccag ttaatcttac aattatcatg
     2821 tcattgagaa tatgtgtacg tataagtttt ggtcctctac ccatatactc aatctcaaat
     2881 ctgctaacta cttcgctgat ttttgcttct aattgtcctt tagtcatcag ttaaccttcc
     2941 agtcattgtt gatatactat attaaataac atcaaatgta aatataatta ttcattgagt
     3001 ttccgtattt tatcttgcat ataaataaaa ctgtaattaa tctgtc
//
LOCUS       DAOSXM010000152         3027 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2111
            NODE_23926_length_3027_cov_11.646366, whole genome shotgun
            sequence.
ACCESSION   DAOSXM010000152 DAOSXM010000000
VERSION     DAOSXM010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864178
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3027)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3027)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:24:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,261
            CDSs (total)                      :: 2,229
            Genes (coding)                    :: 2,218
            CDSs (with protein)               :: 2,218
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 3 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3027
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23926_length_3027_cov_11.646366"
                     /isolate="AMR_MDS_2111"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..735
                     /locus_tag="PLQ68_11135"
     CDS             <1..735
                     /locus_tag="PLQ68_11135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK73245.1"
                     /translation="TIYVQYFSRLDSVEGAIWTDINDSITYDDNVGVPGEHKGYIMII
                     AGTGYDDVAYTLFKGTQYIEGPVYGTYNGNAYFEVDLEPGINDIGILAEVVMNGNYYY
                     ADFKRIEVDYVGNTVNSSTPTPTQSPSPSPSPSEIVNYTPEYMVGTYSYDSNNKEDEM
                     TYIHVNCFIEIYSDGTMRDSGTWQGINEWSNQSSSHDYKETWSIVDNKLVSVNDSGFT
                     RVWDINGDYISYDNGFGYTITYKKMN"
     gene            885..2366
                     /locus_tag="PLQ68_11140"
     CDS             885..2366
                     /locus_tag="PLQ68_11140"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HXK73246.1"
                     /translation="MAKYDFVVIGSGGGLMFIEGALGYNKKCAIVENSKFGGTCLTKG
                     CIPSKMLVYPADLIREAEEGEKIGINFSKPEVDWNKVSKRVWDQINLNVRVEARLSKI
                     PNLDVYKGIGEFSGPHTMRVKNSDGEYIAEFEADNFVIAAGAHSFVPPIEGLKEAGYL
                     TSETFFGDKYPKKPYGSLAVIGAGPIGAEFAHIFASFGTKVSLIEMNPMILSTEEEDI
                     SELVKRQFIKDGMDVYTSTKTLKVSTKDNKKIIHLQNMITSTESIVECDEIFLASGVR
                     SNAPSLKLENAGVETDPRGYIVTNEYLETNVSHIWAIGDVNGRYQFRHKANKEAEILI
                     HNLFKDPNNRKVMRYDAVPWAIFTHPQVGHVGKTEREVKASGCKYKVGINRYSDVTAG
                     RAMGYTSKSDVNGFVKLIVGEDKSILGAHVVGPNASILLQPYVYMMNCDKKCDKKPKK
                     KKVEIEQLRTMCPHLGTYTPMDESMVIHPAMSELTAWVTEDLE"
     gene            complement(2378..2842)
                     /gene="ybaK"
                     /locus_tag="PLQ68_11145"
     CDS             complement(2378..2842)
                     /gene="ybaK"
                     /locus_tag="PLQ68_11145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006001597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HXK73247.1"
                     /translation="MEKTNVMRLLEANCVEYSVYTYDPLVTDGETVANLVGKEADMVF
                     KTLVTIGSDKNNYVFVIPVNCSLNLKKAAISVGVKSISMITLKELLPLTGYVHGGCSP
                     IGMKKKFTTVVNDTALLFDKICFSAGKRGMQIEVNPEDLRKITTHTYSDITD"
BASE COUNT         1081 a          448 c          620 g          878 t
ORIGIN      
        1 accatatatg tacagtattt ttcaaggctt gattcagtag agggagctat atggactgat
       61 attaatgaca gtattacata tgatgataat gtcggagtgc caggagagca caaaggatat
      121 attatgatta tagcaggtac aggttatgat gatgtagcct atactctgtt taaaggtact
      181 cagtacatag aaggcccggt atatgggaca tacaatggga atgcatattt tgaagtggat
      241 ttggagcctg ggataaatga tataggtata ctggctgaag ttgttatgaa tggtaattac
      301 tattatgccg actttaaacg aattgaagta gattatgttg gcaatactgt aaattcatct
      361 actccaactc caacacagtc accatcacct tcaccatcac catcggaaat agtaaactat
      421 actcctgagt atatggttgg tacatattca tatgacagta ataataagga agatgaaatg
      481 acatatatcc atgttaactg ttttattgaa atttattcag atggaacaat gagagattct
      541 ggaacatggc agggaatcaa tgaatggtca aatcagagtt cttcgcatga ttacaaagaa
      601 acatggagta ttgtggataa caagctggtt agtgttaatg attccggatt tacgcgtgtt
      661 tgggatatta atggtgatta tataagttat gacaacggat tcggatacac aattacttac
      721 aagaaaatga actagtataa ttttttcata gtgaaactgc aaccaaaaag agaaagcatg
      781 ttcccgacat gcttttctct taattagata ttgtactgaa tatcggttgt aaatagacca
      841 taggtataat aaaatatatg tgacaaaaac aaatggaggt atatatggct aaatatgatt
      901 tcgtcgttat tggcagcggt ggaggactga tgttcattga aggcgcatta gggtacaaca
      961 aaaaatgtgc aatagttgaa aactcgaaat ttggaggcac atgcttaaca aaaggatgca
     1021 ttccttcaaa aatgcttgtt tatccagcag acctcattag agaagctgaa gagggagaaa
     1081 aaatcggcat aaatttttcg aaacctgaag tagactggaa taaggtttct aaaagagtct
     1141 gggatcagat aaatttaaat gtaagagttg aagcacgact gagtaaaata cctaatctgg
     1201 atgtatataa agggataggg gagtttagcg gtcctcatac aatgagggtt aaaaacagtg
     1261 atggtgagta tatagctgag tttgaagcag ataattttgt gattgcagca ggagctcatt
     1321 cattcgttcc gcctattgaa ggactaaaag aagctggtta tctcacttct gaaacttttt
     1381 ttggtgataa ataccctaaa aagccatatg gcagtcttgc agttataggc gcaggtccta
     1441 taggtgcgga atttgcccat atatttgcaa gctttgggac caaagtttct cttatagaga
     1501 tgaatccaat gatattgtct acagaagaag aggatataag cgaactggtt aaaaggcagt
     1561 ttataaaaga tggaatggat gtgtatactt caactaaaac attgaaggtg tcaactaagg
     1621 ataataaaaa gattattcat cttcagaata tgataaccag tacagaatct attgttgaat
     1681 gtgatgagat attcttagca tcgggagtac ggtccaacgc gccttctttg aaattggaga
     1741 atgcaggggt ggaaaccgat ccaagaggat atatagttac aaatgagtat cttgagacta
     1801 atgtgtcgca tatatgggca ataggagatg taaacggaag atatcagttc cgccacaaag
     1861 cgaataaaga agcagagatt ctcatacata atctttttaa agatccgaat aatagaaagg
     1921 taatgcgata tgatgctgtg ccatgggcaa tatttacaca tccacaggta ggccatgtcg
     1981 ggaagactga gcgggaagtt aaagcatcag gatgtaagta taaagtgggg ataaacagat
     2041 attctgacgt tacagcggga agagcaatgg gatataccag taaatctgat gttaacgggt
     2101 ttgttaaact tatagtcgga gaagataaat ctatattagg agctcatgtt gtaggtccta
     2161 atgcttctat tcttttgcag ccttatgtct atatgatgaa ctgtgataaa aaatgtgata
     2221 agaaacctaa aaagaaaaag gttgagatag aacagctaag gacaatgtgc cctcatttag
     2281 gaacatacac cccaatggat gaatcaatgg taatacatcc tgctatgagt gaactgacgg
     2341 catgggtaac tgaagatttg gaataaaata gtaataatta atcagttata tcagaatagg
     2401 tatgagttgt aattttcctt aaatcttcag gattaacttc aatctgcatt cctcttttgc
     2461 ctgcactgaa gcatatctta tcaaataaaa gggcagtatc gtttacaact gtagtaaatt
     2521 tctttttcat cccgataggg gagcagcctc cgtgtacata accggtaaga ggtaataact
     2581 ctttcaaagt aatcatagaa atgcttttaa cccctacaga aatagcagct ttcttaagat
     2641 ttaaagaaca attaacaggt atcacaaaca cataattgtt tttatcagaa ccaatagtaa
     2701 caagagtctt gaagaccatg tctgcttctt tacctaccaa attagctact gtttcaccat
     2761 cagtcactaa aggatcataa gtataaacag aatattcaac acagtttgct tctaaaagcc
     2821 tcatcacatt agttttttcc atatagtgat aatacaatat agagacaaaa aaataaatga
     2881 tatatcaaca aaaacctcct tgcatcacta aggaggttta tgccatttca tgaaaagatc
     2941 aaataatgaa ataaaaatat aaaaagaata gtatctatca aatacattat acaacagatt
     3001 tagtaaaatg tttagtgttt taaaaaa
//
LOCUS       DAOSXM010000153         3023 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2111
            NODE_23970_length_3023_cov_8.640836, whole genome shotgun sequence.
ACCESSION   DAOSXM010000153 DAOSXM010000000
VERSION     DAOSXM010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864178
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3023)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3023)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:24:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,261
            CDSs (total)                      :: 2,229
            Genes (coding)                    :: 2,218
            CDSs (with protein)               :: 2,218
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 3 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3023
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23970_length_3023_cov_8.640836"
                     /isolate="AMR_MDS_2111"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(116..1312)
                     /locus_tag="PLQ68_11150"
     CDS             complement(116..1312)
                     /locus_tag="PLQ68_11150"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HXK73248.1"
                     /translation="MGKKLNVKIKAIGLLDLLAFKKLVPNRKKKFEEAPATEIKKIPP
                     MNELAAKLHPKHQKFTITKVTDLTDSMKQFELTSDSAAFFRAGQYISVKLDIDGHKVT
                     RPYSIMSSPKASLSGKYMLGIKKADDGFVTEYIFNNWKEGTAIETSGPEGNFYYEDIR
                     DAKTVIGIAGGCGITPFFSMAQAIRDGIEDFNLCLLYGCKTLDESALKKELDEIAETD
                     SRVKVVCVLSNEEKEGYEHGFITADIIKEYAGTEPYSVFMCGPQVMYDFVGKEIDKLG
                     LEKKFVRRELYGQINNVEKHEGFPKDKVGKKFKLTVHIGQNTITTEALSTESVLVAVE
                     RAKIIAPSLCRTGECGCCRSRLISGDVFIVEDTDGRRMADKKFGYIHICSSFPLSDLE
                     IEIPVV"
     gene            complement(1315..2850)
                     /locus_tag="PLQ68_11155"
     CDS             complement(1315..2850)
                     /locus_tag="PLQ68_11155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007045673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HXK73249.1"
                     /translation="MSEQTYDVVVIGAGNGGLAAAATTAQKGMKTLLIEQHTLPGGYA
                     SSFVRGRFEFEPSLHELCDVGKEGEWGACGTFFKDLGLDIEWVPVPEAYRMVITDKNE
                     KLDVTMPFGLKEYIDKMEEYVPGSRESVTNFFDLCQEVLDAIGYLGASKGNPDKKVLT
                     SKYSNFLKTASYSVDQVEKALKIPKKARDILNAYWCYLGTDTGKLGFTIYSAMMIKFL
                     LLGAYIPKMRSHEISLAFEKAIRDFGGDVIYGTKVEKILVENGSVVGIKTDKGEKINT
                     KHVICNAPRHVAFGSMVEGADEIKEKTNREFNARKVGGKGYVAYFGLNKTAKELGITD
                     YSYFIYPTVDNKKLYDSMFNRGKNPMQAIVCLNNAIPDCSPPGTSMLTVTTLYKDGAW
                     DDVNETNYVQAKNEIAKDIVKNIESALNIKISDAIEEFSVASPETFARYACAYDGSIY
                     GYESETWDSLLPRLLMMKEDLYINGLRFAGGFAFRCHGYSSGYMSGNTIGLLTYKDSL
                     EEK"
BASE COUNT          813 a          715 c          481 g         1014 t
ORIGIN      
        1 ctcattacaa gtaaattatc agtctgcaaa atcaaatagt caatactaat atattttacg
       61 ttctaatgtt atccagctgt taaaattcta aacagtttat gcaaaactgc aagtcttata
      121 caaccggtat ttctatttcc aggtctgaaa gagggaaaga actgcagata tggatatatc
      181 cgaacttttt atcagccata cggcgtccgt cagtatcttc aactatgaat acatcaccag
      241 atatgagtct gcttctgcag catccgcatt cacctgttcg acaaagtgaa ggtgcaatta
      301 tttttgcgcg ttcaacagct accagcacgc tctctgttga taaggcttct gttgttatag
      361 tgttctgtcc gatatgtact gtaagcttga atttctttcc aactttatcc ttcggaaatc
      421 cctcatgctt ctcaacatta ttgatctgcc catacagttc acgtcttaca aacttctttt
      481 ccagtcctaa tttatctatc tctttaccga caaaatcata catgacctga ggaccgcaca
      541 taaatacgga ataaggttca gtccctgcat actctttaat aatatctgct gttatgaatc
      601 cgtgctcata tccttctttt tcttcattac ttagcacgca tacgacctta accctgctgt
      661 cagtttcagc tatttcatca agttccttct tcaatgcgct ttcatcaagt gtcttacatc
      721 catacagaag acagaggtta aagtcttcta tcccgtccct tatagcctga gccatagaga
      781 aaaacggtgt aattccgcaa ccgcctgcaa taccgataac tgtctttgca tcacggatat
      841 cttcataata gaagtttcct tcaggtccgg atgtttcaat agcagtccct tccttccagt
      901 tattaaatat atattctgtt acaaaaccat catcagcctt ttttattcca agcatgtatt
      961 ttcctgataa tgacgctttt ggcgagctca taatagaata tggcctggta actttatgcc
     1021 cgtcaatatc cagcttcacg cttatatact gtcccgctct gaaaaatgca gctgaatcag
     1081 aagtaagctc gaactgcttc atgctgtctg tcagatcagt aacttttgtt atcgtgaact
     1141 tctggtgctt tggatgcagt tttgcagcca gctcgttcat aggcgggatt ttctttattt
     1201 ctgtagcagg cgcttcttca aacttctttt ttctgtttgg taccagtttt ttaaatgcca
     1261 gcagatccaa aagccctatt gcttttatct ttacgtttaa tttcttaccc ataatcactt
     1321 ttcctccaaa ctgtctttgt acgttaaaag cccgatcgta tttccagaca tgtaccctga
     1381 actgtaacca tgacatctga aagcaaaacc gcctgcaaat cttaatccat ttatatacag
     1441 atcttccttc atcatcagaa gacgaggcaa cagtgaatcc catgtttctg attcatatcc
     1501 atatatactg ccgtcatatg cacatgcata tcttgcaaaa gtctcgggag atgctacact
     1561 gaattcctct atcgcatcag aaatcttaat attaagcgct gattcaatat ttttaacaat
     1621 atctttcgct atctcatttt tagcctgtac ataatttgtt tcgttaacat catcccatgc
     1681 accatctttg taaagagtgg taactgtcag catgcttgtg cctggaggag agcagtcagg
     1741 tattgcatta ttaagacata caattgcctg cataggattt tttcctctat taaacatgct
     1801 gtcatacagt ttcttgttat ccaccgtcgg atatatgaaa taactgtagt cagttatccc
     1861 tagttctttt gctgtcttgt taagcccgaa atatgccaca tatccttttc cgccgacttt
     1921 tctggcgtta aattcccggt tcgttttttc ctttatttcg tccgcgcctt caaccatact
     1981 tccaaatgcc acatgccttg gagcattaca gatgacatgc ttcgtattaa tcttttcacc
     2041 tttgtcggtt tttattccta caactgaacc gttctcaacc agtatctttt caaccttggt
     2101 tccatatatg acatctccac caaaatcacg gatagccttt tcaaaagcta acgaaatctc
     2161 atgtgaacgc atcttcggga tatatgctcc taataaaagg aactttatca tcattgcgct
     2221 atatatggtg aagccgagtt tcccagtgtc ggtacccaga tagcaccagt atgcatttaa
     2281 aatatcacgt gctttttttg gtatctttaa tgctttttct acctggtcaa cactgtatga
     2341 tgccgttttt aggaagttag aatacttgga agtcagaacc tttttatcag gatttccttt
     2401 tgatgctcca agatacccta tagcatcaag tacttcctga cacaaatcaa agaaatttgt
     2461 tactgattcc ctgctcccag gcacatattc ttccatctta tcaatgtact ctttcaaccc
     2521 gaaaggcatt gtaacatcaa gcttttcgtt tttatcagtt ataaccatgc ggtaagcttc
     2581 cggtaccggg acccattcga tgtccagtcc aaggtcctta aaaaacgtac cacatgcgcc
     2641 ccattcccct tctttgccca catcacacag ttcatgcaga gacggctcaa attcaaacct
     2701 tccacgaaca aaactgcttg catagcctcc tggcagagtg tgctgctcaa taagcagcgt
     2761 cttcattcct ttttgtgctg tcgtagctgc agcggccaga ccgccattcc ctgctccgat
     2821 aacaacaaca tcgtaagttt gctcactcaa aatgtgtcaa cccccttctt tttatggttt
     2881 tgtcaaaagc catattcata ttgcgtattt aaattgtact gcttggtcat attatatgtt
     2941 tatctgacaa ataattcaag ttgaacatag tttgatagtg taaacttgtt tggggattga
     3001 taaaataaaa gagaaatccc att
//
LOCUS       DAOSXM010000154         2990 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2111
            NODE_24382_length_2990_cov_10.966951, whole genome shotgun
            sequence.
ACCESSION   DAOSXM010000154 DAOSXM010000000
VERSION     DAOSXM010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864178
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2990)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2990)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:24:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,261
            CDSs (total)                      :: 2,229
            Genes (coding)                    :: 2,218
            CDSs (with protein)               :: 2,218
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 3 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2990
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24382_length_2990_cov_10.966951"
                     /isolate="AMR_MDS_2111"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1520)
                     /locus_tag="PLQ68_11160"
     CDS             complement(<1..1520)
                     /locus_tag="PLQ68_11160"
                     /inference="COORDINATES: protein motif:HMM:NF013106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HXK73250.1"
                     /translation="MNEEELKNLYEDEEEDIQNVEYVEYAPSFLSTFFSTFWAALKIL
                     LAVILIAGFLAGGLGLGIITAWISTSKILTDEDLAITTGLTTFIHDSEGNIIGTLTGS
                     DNINREVITTKQIPEVLAHAIVAIEDERFYQHSGFDFIRIGGSIVKLIQNRGDIAQGG
                     STITQQVYKNITGRFEQTFERKIQEVYNAVLLEMKYDKKQIITMYANLVNMGNGCYGY
                     QSASKLYFGKTLDEINLSEAAVLAAITNSPGTYDPYGNEEKIRKLMFRREDVLDKMLE
                     LGYIDQREYDEALAYEIVFAEKVEYVSSSITSYFLDYVITEAIDILSTQNSISELQAE
                     RIIYNNGLHIYTTLVPHVQDSLDTIFTDDSYFAQYREDGSIINIDAIRYGEKPQGAMV
                     IIDQYTGGIAGLYGGNGEKTGSRTYNRATGMQRQPGSTFKPLAVYGPALDLKLITAST
                     IVDDAPAYLDPKNPDLIYPTNYDSTDYQGLTTIRNAIKASVNVVAAKVWSYYLGRD"
     gene            complement(1596..1955)
                     /locus_tag="PLQ68_11165"
     CDS             complement(1596..1955)
                     /locus_tag="PLQ68_11165"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HXK73251.1"
                     /translation="MIRQKKLDVLIGPMCTIKGDISTKGSVTIEGTIIGDINAEGDIV
                     FTKSAKAKGQFIGANIILSGDAEGILKSKNYIKMSSSANFVGDTYALSLISDEGAKFT
                     GKCYVLEDKEKDDKEKK"
     gene            complement(1965..2927)
                     /locus_tag="PLQ68_11170"
     CDS             complement(1965..2927)
                     /locus_tag="PLQ68_11170"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HXK73252.1"
                     /translation="MRKGRTDLDGEGMLVYIPKGGKSSKVLKVTSPILKLSLIFYFLM
                     AIIVCLSLSLLYFINENKQVNAQMEAIVADYNTEMITLSTYVGKQAQLLDSKLNEIAT
                     LETVQEDLNIQIKDLSLQLKNIAERYTDSISLASASVTANNVAKFAEDAANIAEALEI
                     LLETNENTKSELVDFTNAANILNEYLISVPTYWPTKSTYIASAFGMRNHPILNQYKQH
                     TGVDIGGLWGDEIYAAATGTVLSVRYEQSGYGYWLRIKHSESITTLYAHCSKILVKAG
                     DTVEKGQVIAYVGSTGLATGPHLHFEIRVNDIPINPTLFITSSK"
BASE COUNT          867 a          593 c          444 g         1086 t
ORIGIN      
        1 ttatccctgc caagataata agaccacacc tttgctgcta caacgtttac tgatgctttt
       61 atagcattac ggatggttgt taaaccttga taatcagttg agtcgtagtt ggtaggatat
      121 ataaggtcag gattcttagg gtcaagataa gctggagcat catctactat tgttgaagct
      181 gttattaatt taagatctaa cgcaggtccg tatactgcta aaggtttaaa ggttgaacca
      241 ggctgccttt gcattcctgt agctctgttg tatgttctgc ttcctgtttt ttctccatta
      301 cctccataca gccctgctat acctcctgta tactgatcta ttataaccat cgcaccctga
      361 ggcttttcgc cataacgtat tgcatcaata ttaattatgg aaccgtcttc cctatattgt
      421 gcaaaatatg aatcatctgt aaaaatagta tctaatgaat cctgaacgtg aggcactaat
      481 gtagtataga tatgaagccc attattgtat attattcttt ctgcttgcag ttcggaaata
      541 gaattctgag tagacagaat atcaattgct tctgttataa cataatcaag aaaataactt
      601 gttattgatg atgatacata ttccactttt tctgcaaata ctatctcgta tgccaatgct
      661 tcatcatatt ctctttggtc tatataaccc aattccagca ttttatccaa aacgtcctca
      721 cgcctgaaca ttaatttcct tattttctct tcattgccat aagggtcata tgtaccaggc
      781 gaattagtaa tagctgcaag gacagctgct tcagaaagat ttatttcatc taatgtcttt
      841 ccaaaataca atttggatgc tgactgataa ccataacagc cgttacccat atttacaaga
      901 ttagcataca tggtaataat ctgttttttg tcatacttca tttctaaaag tacagcattg
      961 tatacctctt gaatctttct ttcaaatgtc tgttcgaaac gacctgttat atttttatat
     1021 acctgctggg taatagtact tcctccctga gcaatgtcgc ctctattctg tataagtttt
     1081 acaatagatc cacctattcg tataaagtca aatccagaat gctggtaaaa tctttcatct
     1141 tcaattgcaa caattgcatg tgctaatact tcaggtatct gttttgtcgt tataacttct
     1201 ctgtttatat tatcgcttcc agtaagtgtt ccaataatat ttccttcact atcatgaatg
     1261 aatgttgtaa gacctgttgt gattgccaga tcttcatctg ttagtatttt tgaagtggaa
     1321 atccatgcag ttataatccc tagaccaagg cctcctgcta aaaagcccgc aataagtata
     1381 acggctaaaa gaatttttaa tgcagcccag aaagtactga aaaaagtgct taaaaaagaa
     1441 ggggcatact ctacatattc aacattttga atatcttctt cctcatcttc gtataagttt
     1501 ttcaattctt cttcgttcat ctcactacct cattagcata agagctttct tatgctcaaa
     1561 aaatattctg cgtgctatat gcatacagaa atttattatt ttttttcttt atcatctttt
     1621 tctttgtctt ctagcacata gcattttcct gtaaatttcg caccctcatc acttatgagg
     1681 cttaatgcat atgtatctcc tacgaaatta gcagaagaag acatttttat gtaatttttt
     1741 gactttaata ttccttcagc atctccactt aatatgatgt tagctccgat aaattgtcct
     1801 ttggcttttg ccgattttgt gaaaactata tcaccttctg catttatatc gcctataata
     1861 gttccttcga tagtaacaga tccttttgtt gaaatatcac cttttattgt acacattggt
     1921 cctattaaaa cgtccagctt tttttgccta atcatttttt tctcctactt tgatgaagtt
     1981 atgaaaagtg tcgggtttat aggtatatca ttaaccctga tttcaaaatg aagatgtggt
     2041 cctgtcgcaa gccctgttga acccacataa gcaataacct gacccttttc tactgtgtct
     2101 cctgctttaa ctaaaatttt tgaacagtgg gcatacaatg ttgttatgct ttcactatgt
     2161 ttaattcgaa gccagtatcc atatcctgac tgttcatatc ttactgataa aacagtccct
     2221 gtagcagctg cgtaaatctc atctccccat aaaccaccga tatctactcc agtatgctgt
     2281 ttatattgat tcaatatagg atgatttctc attccaaagg cagatgcaat gtatgttgat
     2341 tttgttggcc aataagttgg taccgatatt aaatattcat taagtatatt agcagcatta
     2401 gtaaaatcaa caagttctga ttttgtattc tcatttgttt ccagcaaaat ttccaaagct
     2461 tctgcaatat tggctgcatc ttctgcaaac ttggcaacat tattagctgt aactgaagcg
     2521 ctggctaatg atatgctgtc agtatatctc tccgcaatat tcttaagttg aagcgataaa
     2581 tcctttatct gtatatttaa atcttcctga actgtttcca gtgttgcaat ttcatttaat
     2641 ttactatcca gtaactgagc ttgtttcccg acataagtgc ttaaggtaat catttcagtg
     2701 ttgtaatctg ctacaatagc ttccatctga gcatttacct gtttattctc gttaataaaa
     2761 taaagcaaag acaaactcag acatactata atagccatta gaaagtaaaa tattagtgat
     2821 aatttaagaa ttggtgatgt aacctttaag acttttgaag atttcccacc cttagggatg
     2881 taaacgagca taccttcgcc gtctaaatct gttctacctt ttcgcatcta tcctcctcat
     2941 acttataaaa gatactatca tatgaatata cttttttcaa cagtttatat
//
LOCUS       DAOSXM010000155         2891 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2111
            NODE_25728_length_2891_cov_8.665021, whole genome shotgun sequence.
ACCESSION   DAOSXM010000155 DAOSXM010000000
VERSION     DAOSXM010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864178
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2891)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2891)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:24:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,261
            CDSs (total)                      :: 2,229
            Genes (coding)                    :: 2,218
            CDSs (with protein)               :: 2,218
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 3 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2891
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25728_length_2891_cov_8.665021"
                     /isolate="AMR_MDS_2111"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1510)
                     /locus_tag="PLQ68_11175"
     CDS             complement(<1..1510)
                     /locus_tag="PLQ68_11175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HXK73253.1"
                     /translation="MGRIENASKNIRYGYLGVLLTLLLQFVSRTIFIHTIGVTFLGVS
                     GLYTNVLSVLSFAELGIGTAMNFSLYKPVAENDKDKIKSLMQLYKYAYRWIALVVTVF
                     GLGVIPFLNFIIKEPGAITPNELIIYYVIFLFNTVSTYFVAYKYSLVNAEQKNYIQTN
                     IRTVTLIVTTVIQILVLLIFKNFLFYLLTAAAVELFQKIYANWYLNKRYPYLLDKDVR
                     KLSKEDKLPIVKNIKAMVYHKIGEISVHQTDNILISSFINISTVGIVSNYTLIITSVT
                     SFINIVFNSLISSFGNLIATENVDKQYSLFKVYRFAGFWLYGFASVAFVVLLNPFVQL
                     WIGHEMLVGTVVIYLIIIDYYFRGHRIVVNNFKIAAGIFDEDKYIGIIQAVVNLVVSI
                     IMVKQIGLPGIYIGTLAQGLISTLVKPRIVYKNVFKRSVKEYYTDSVKYLVIILIPLV
                     ILELIKLYLLPDITILNFIILVILVTVIPNGIILLACRKREEFIYILNLVKQK"
     gene            complement(1530..2657)
                     /locus_tag="PLQ68_11180"
     CDS             complement(1530..2657)
                     /locus_tag="PLQ68_11180"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HXK73254.1"
                     /translation="MDKIGIITLNGYFNYGNRLQNYALQETLKSFGYEVETVIVDDRR
                     LDMSTSEVIAKKLNILKEESLSGTFRIISKKLKDRLNQEAIAKRTKLFKDFTNRHISE
                     SKYKVTQKGLDEKYEKTYSFFVAGSDQIWNPKYVNLPEIYFLTFTEKKKRIAYAASFG
                     ISNIPSEFKERYVEYLNGMSYISLREGDGAKIVEELTGNKAPVLLDPSLLLNKQQWLD
                     ISKKPDNIADNMKGKYLLTYFVEGMDKKTEKEIKKTADRLNLPIINMGDINDKEGYIS
                     GPSQFIWYINNCSVLCTDSFHGTAFSILLQTPFIVYKRAGANSIYSRIDTLLDKFDLN
                     SRKIENIKEDNDLLKVDFTHTVAILKSERKKAENYLKVALK"
BASE COUNT          956 a          519 c          412 g         1004 t
ORIGIN      
        1 tcttctgttt aacaagatta agaatgtata tgaactcttc tctttttctg caggctaaca
       61 gtattatccc atttggaatt actgttacta atattaccaa gattatgaag ttcaatattg
      121 ttatatctgg taacaggtat agctttataa gctcaagtat aactaaaggt ataagtatta
      181 ttacaaggta tttaacagaa tcggtgtagt attctttaac gcttctttta aatacatttt
      241 tgtatacaat acgaggtttt accaaagtag atattaagcc ttgagctaaa gtaccaatat
      301 atatgccagg aagaccaatc tgttttacca ttataatcga aaccactaaa ttcacaacag
      361 cctgtataat cccgatatat ttatcctcgt caaatatacc tgctgctatc ttaaagttat
      421 taaccactat gcggtgtcct cgaaaatagt agtcaatgat tatcaggtag ataactacag
      481 tacctacaag catttcgtgg cctatccata actgcacaaa aggatttaaa agcaccacaa
      541 aggcaacaga agcaaaacca tacagccaga aacctgcaaa cctgtatact ttgaataaag
      601 aatactgttt atccacattt tctgtagcta tgagatttcc aaaactggat atcaaagaat
      661 taaagacaat gtttataaag ctggtaacag aagtgataat caaagtgtaa ttagatacta
      721 tacctactgt agatatgttt atgaaagagg atataaggat attatcagtc tgatgcacac
      781 tgatttcgcc tattttgtga taaaccatag ccttaatatt cttaactata ggcagtttat
      841 cttccttaga aagctttctt acatctttgt ctagtaaata cggatatctt ttattcaaat
      901 accagttagc atatatcttt tgaaacagct ccactgctgc tgctgttaaa agatagaata
      961 aaaagttctt aaagataaga agaacaagta tctgtataac agtagtaact attaatgtaa
     1021 cagtacgtat attagtctga atatagtttt tctgttcagc attaaccaga ctgtacttgt
     1081 aagcaacaaa gtatgtgctt acagtattaa acaggaatat cacgtaatag attatgagct
     1141 catttggtgt aatagcacca ggttctttaa ttataaagtt cagaaacggt ataacgccaa
     1201 ggccgaaaac agtaacaacc agagcaatcc atctgtatgc atacttatat agctgcatca
     1261 aagacttaat cttatcttta tcattttcgg ccactggctt gtacagactg aaattcattg
     1321 cagtgccgat accaagttca gcaaaagata aaacagaaag aacattagtg taaagcccgc
     1381 ttacacctaa aaatgtaaca cctatggtat gaataaatat agttctggag acaaactgca
     1441 ataaaagagt caataagacc ccaaggtatc catatcgtat attctttgaa gcgttctcta
     1501 ttcttcccat aaatacaact ttctttttat tatttcaaag ctacctttaa gtagttttca
     1561 gccttttttc tttcgctctt taaaatagct actgtatgag taaaatcaac tttaagaaga
     1621 tcattatcct ctttaatgtt ttcaattttt ctgctgttaa gatcaaactt atccagtaaa
     1681 gtatctatac gggagtaaat cgaattagct cctgctcttt tgtagacaat aaacggagtc
     1741 tgcaaaagaa tggaaaaagc agttccgtga aaagaatcag tacacaagac agaacagtta
     1801 tttatatacc atataaactg gctaggtccg gaaatatacc cctctttatc atttatatca
     1861 cccatattga ttattggtag atttaatctg tcagcagttt tcttaatttc cttttcagtc
     1921 tttttatcca taccctctac aaaatatgtt aaaagatatt tacctttcat gttgtcagct
     1981 atgttatcag gctttttcga tatatccagc cactgctgtt tattaagtaa aagagacggg
     2041 tcaagaagta caggtgcttt attacctgta agctcttcta ctatctttgc tccgtcacct
     2101 tcccttagag atatatagct cataccgtta aggtactcaa catatctttc tttaaattcc
     2161 gaaggtatgt ttgatatgcc aaaacttgca gcatatgcaa ttcttttttt cttttctgta
     2221 aatgtaagaa aatatatttc cggtaaattt acatacttag gattccatat ctggtcagaa
     2281 cctgctacga agaatgaata agttttctca tatttttcat ccagtccttt ttgagttacc
     2341 ttatatttgc tttctgatat atgtctatta gtaaagtctt taaacagttt agtgcgtttt
     2401 gcaatagctt cctgattaag tctgtctttt aactttttgg atatgatcct gaatgtgcca
     2461 gacaaagatt cttcctttaa aatattcagt ttttttgcaa ttacctctga agtactcata
     2521 tcaagtcttc tgtcatctac tattacagtc tcaacttcat aaccaaaaga tttcaaagtt
     2581 tcctgtaatg cgtaattttg aagcctgttt ccataattaa agtatccgtt cagagttatt
     2641 atacctatct tatccacttc atctccttgt gaaatatacc ttagtgtcat ttcatataac
     2701 taatattaat atataactgg tcaaatatcc atacagcata ttttatcaat gacaaacatc
     2761 attgctataa cctgaatgaa tatatcacct atcttaacta atgtgcttga agcttcattt
     2821 agttaatgac acgttttatt agttattgca ctttttaaat tatataccct gtagcttttt
     2881 gtaacaatac a
//
LOCUS       DAOSXM010000156         2890 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2111
            NODE_25753_length_2890_cov_10.052205, whole genome shotgun
            sequence.
ACCESSION   DAOSXM010000156 DAOSXM010000000
VERSION     DAOSXM010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864178
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2890)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2890)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:24:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,261
            CDSs (total)                      :: 2,229
            Genes (coding)                    :: 2,218
            CDSs (with protein)               :: 2,218
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 3 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2890
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25753_length_2890_cov_10.052205"
                     /isolate="AMR_MDS_2111"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..909)
                     /locus_tag="PLQ68_11185"
     CDS             complement(<1..909)
                     /locus_tag="PLQ68_11185"
                     /inference="COORDINATES: protein motif:HMM:NF020730.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HXK73255.1"
                     /translation="MRKTKKILAVILAISMIAVYITAIPVSAATLTDPEKLAAMNLFR
                     GDTADGLTPQYLAKAPDRKTAARLLLRFNGLEEAADAYTGTATFSDATSATVYWQPMI
                     GYLKANPGAGFGGYEDGTFRPNDAMTAQAFYKVLLTILGYKQDTDFTWAQTFTFAASK
                     GMTKIASKTGITNADVATAMVEALNATTKTGDKLITALINDGVVTATQAAAAGFNVIS
                     TVTGYETAYTMAYGGANPLPATVTAVYQDGNTASVAVAWGSFSTTVEGTYDVTGTIEG
                     FGTITTKVTLTKTLMVTDAYPSGTKEI"
     gene            complement(1096..2457)
                     /locus_tag="PLQ68_11190"
     CDS             complement(1096..2457)
                     /locus_tag="PLQ68_11190"
                     /inference="COORDINATES: protein motif:HMM:NF024817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HXK73256.1"
                     /translation="MKKYNAFVEKLFWLMIIINPFLDAATGIYKTFFEKEDAAFSLSP
                     SLIIRFIFLSLLIVYIFSTKNKKAIFTLIPIGIAAVASVGVEIILNMDFSIMEDAMYI
                     ARYAYNIIMLFCWYWIFSKLGYNKKDLLGKLNRLLCWTLIIIGLNVIIGQITGTGHST
                     YADRFGYRGSKGYYFAGNDITAILMLLFPVSIYAFINSTRTNKKDRNLFLIAAAMTMV
                     TNSLIGSKTSFLALFATSAVVIIYGLWVLIKDKNSLYIKKFGVIVLTAVIMAGAIIGL
                     EYLQKESSFAQTIKESIGSTGLIIEREGIETALLSGRQVKLEKALTEYKEAGPLAWVF
                     GIGRGSQEGIIEMDVLEVLCYYGIFGVVAMLWIYVALGFILIKKFFKNFSFETLMVVT
                     AIALCAGYMLIAGHILFSVTSGYYFTMMFAYSELVYSKDIRDLPDRIPFPFKKLLQTK
                     KVS"
     gene            2589..>2890
                     /locus_tag="PLQ68_11195"
     CDS             2589..>2890
                     /locus_tag="PLQ68_11195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HXK73257.1"
                     /translation="MAFITKKELTGHLAPSSDNQRNSEGDFISIDNDKVLFAYSRFSG
                     QNHHDHDPSNIASVVYDLNSYTFDSNAEIVKKASDFGVQNLMSVSLLRMLNGDIGM"
BASE COUNT          882 a          551 c          499 g          958 t
ORIGIN      
        1 tatttcctta gttcctgaag gatatgcatc agtaaccatc aatgtcttag tcaatgttac
       61 ttttgttgta atagttccaa aaccttcgat tgtacctgtt acatcatatg ttccttcaac
      121 agttgttgag aatgaacccc atgcaactgc gactgatgca gtgttgccat cttgatatac
      181 tgctgtaact gtagcaggaa gtggattagc accgccatat gccattgtgt atgcagtctc
      241 ataacctgta actgtcgaga taacattaaa tccggctgct gctgcctgtg tggctgttac
      301 aacaccgtca ttgataagag cagttataag cttgtctcct gttttagttg tagcattcaa
      361 tgcttcaacc attgctgttg caacatcagc attagttatg ccggtttttg acgcaatttt
      421 agtcattcct tttgatgcag caaacgtaaa tgtctgagcc catgtgaaat cagtatcctg
      481 cttatatcct aatattgtaa gcagaacttt atagaaagct tgggctgtca tagcatcatt
      541 aggtctgaat gtgccgtctt catatccacc gaaaccagca cctggattag cttttaaata
      601 gcctatcatc ggctgccaat aaactgttgc agatgtagca tcagaaaagg tagctgtacc
      661 tgtataggca tcagcggctt cttcaagacc attaaatctg agcagtaaac gagcagcagt
      721 ttttctgtcg ggagcctttg caagatattg cggtgtaagg ccatcggcag tatcgcctct
      781 gaatagattc atagcagcta gtttttcagg atctgtcaat gttgctgctg ataccggaat
      841 tgcggtaatg taaacagcga tcattgagat agccagaata actgctaaaa ttttcttggt
      901 ttttctcatt attgtcgttc ctccttgtta gttacgaatt ttcttttgta caagcgttag
      961 tttttattgg acgtatctga aactgtttgg gttccatatt atttcacaat atttttaacg
     1021 ccatatattg caattatatt ttattatgca agtaaagtaa acagttaaat ttcactttac
     1081 taggctgtaa ccatatcagg acaccttctt agtctgtaat agttttttaa aaggaaaagg
     1141 tattctgtct ggcaaatcac gaatatcctt tgaatatact aattcggaat atgcgaacat
     1201 catagtaaaa tagtaacctg atgtgactga aaacaatata tgtcccgcta taagcatata
     1261 ccctgcacat aaagctatag ctgttacaac cataagtgtt tcaaaactga agttcttgaa
     1321 aaacttcttt atgagtataa atccaagtgc tacatatatc cataacatag ccacaacacc
     1381 aaatatgcca tagtaacaga gtacttccag tacatccatt tctattatgc cttcctggct
     1441 tccccttccg attccgaata cccaggccaa cggtcctgct tctttatact ccgtaagagc
     1501 tttttcaagc ttaacctgtc gcccgcttag aagggctgtc tcaataccct ctctttcaat
     1561 tatcaatcct gtacttccta ttgattcttt tattgtctga gcaaaagagg actctttctg
     1621 taaatattca agacctatta tagctcctgc cataataact gcagtaagca ctatcacacc
     1681 gaattttttt atgtataaac tgtttttatc cttaatcagc acccataatc cataaatgat
     1741 tacaacagct gatgttgcaa acagggcaag aaaacttgtt tttgatccaa tcagactgtt
     1801 tgttaccata gtcattgctg ctgctattaa aaatagattt ctgtcttttt tgttagttct
     1861 tgtcgaattg ataaaagcat atatcgagac tggaaataaa agcattaaga ttgcggttat
     1921 gtcattacct gcaaaatagt atcctttaga acctctgtat ccgaatctgt cagcatatgt
     1981 agagtggcct gtacctgtaa tctgtcctat tattacattc aggccaatga ttataagggt
     2041 ccagcataat aacctgttca gtttgccaag aagatctttt ttgttgtaac ccagttttga
     2101 aaatatccag taccagcaaa acagcattat tatgttatat gcatatcttg ctatatacat
     2161 ggcatcttcc attatcgaaa aatccatatt caggattatc tcaacaccta ctgaggcgac
     2221 tgccgctatt ccaataggta ttaaagtaaa tattgctttt ttgttttttg tggaaaatat
     2281 atatactatc agcaatgata aaaatatgaa tcgaataatg agacttggcg acaaggaaaa
     2341 tgctgcatct tctttttcaa agaatgtttt gtatattcct gtagctgcat caagaaacgg
     2401 attaattatt atcattagcc agaacaattt ctcaacaaat gcgttatact ttttcatatg
     2461 tcctccgaaa gatatgcttt taagaatata tcacacaaga acaacagagt aaagtggata
     2521 aaaatcatac ttgcttttgt tgtctgaaag actggttggt gttaaactta aaaaaaaaga
     2581 ggtgtagtat ggcgtttata acaaaaaaag aattaacagg gcatttagct ccttctagtg
     2641 acaatcagag aaattcagaa ggcgacttca tatcaataga taatgataag gtactatttg
     2701 catatagcag gttttccgga caaaatcatc atgatcatga cccgtcaaac atagcttcag
     2761 tcgtatatga cttaaactcc tatactttcg attctaatgc tgaaatagtt aagaaggcat
     2821 ctgattttgg tgttcaaaac ctaatgagtg tatctttatt aagaatgtta aatggtgata
     2881 taggtatgtt
//
LOCUS       DAOSXM010000157         2882 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2111
            NODE_25869_length_2882_cov_8.491334, whole genome shotgun sequence.
ACCESSION   DAOSXM010000157 DAOSXM010000000
VERSION     DAOSXM010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864178
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2882)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2882)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:24:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,261
            CDSs (total)                      :: 2,229
            Genes (coding)                    :: 2,218
            CDSs (with protein)               :: 2,218
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 3 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2882
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25869_length_2882_cov_8.491334"
                     /isolate="AMR_MDS_2111"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..295
                     /locus_tag="PLQ68_11200"
     CDS             <1..295
                     /locus_tag="PLQ68_11200"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HXK73258.1"
                     /translation="FESETDTAIREVWEETGIAVIPDIRFRRSVTYNIQPVYTKEVVF
                     YISNYTGEVNVNKSEIDEAGWFNMSEAKRRLRYMELCSVLEDAEEYIGNLIEN"
     gene            449..1876
                     /locus_tag="PLQ68_11205"
     CDS             449..1876
                     /locus_tag="PLQ68_11205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009253246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXK73259.1"
                     /translation="MLESELIRLVKKITTEKCEKQNLELKKASNGTPEKLYSTLSSFS
                     NQIGGGIIVFGIDENEGYDIAGVYDAQDLQKKVGEQALQMEPVVRPLFTVAQINGKLI
                     VSAEISECDIFDKPCFYKGSGRLRGSYIRVGDSDQPMTEYEIYSYEAYKRRIHDELRT
                     VDKATLKYLSKDRIAEYLIKIRRQKANLVNLSDERLLETQGIVKNGLLTITGLMLFGE
                     YPQEFFPQLSVTAMAVQGRAIGELGKDNERFIDNRRIEGTIPQMLEDALSFIRRNMKI
                     KTIINKDGVRADKYEYPLVAVREIVLNALIHRDYSIHTERSPIRLVMYDDRLELENPG
                     GLYGRITVNDLGKVAADTRNPYMAGALEILETAENRFSGIPTVISELKKAGMPAPVFI
                     DKRGVFKVIFYKEDAIQNSISELEQMILEYCETPRSREELVVKFKFDSVSYFMKTYIY
                     PLVEKGMLKMIFPEKPRSKFQKYYS"
     gene            1930..2694
                     /locus_tag="PLQ68_11210"
     CDS             1930..2694
                     /locus_tag="PLQ68_11210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017415354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4111 domain-containing protein"
                     /protein_id="HXK73260.1"
                     /translation="MEESIDIMVKSITDILHDNCLSVYLYGSVCEDDFRLGWSDIDIL
                     CLTKASVRQEDALRLVDLRKSLSNMYPDNQYFSLFEGCILSLNSFINKTEDTCVYWGS
                     SKSRITTRYDFNSLSMTQLIKSSKLLYGKDILHIFTLPSYDELRNDVINHYETIRKYA
                     KSTSRSIHSYGWLLDISRCIYTIQTGKIISKTRAGEWALEYNLCPVQEPLEKAVRVRK
                     DPYKYHSDQEILDYSEGLGEHIQKYADVLEEVIKYS"
BASE COUNT         1049 a          374 c          592 g          867 t
ORIGIN      
        1 ttttgagagt gagactgata ccgctataag ggaagtttgg gaagaaacag gcatagcagt
       61 aataccggat atccgattca gaagatcggt tacatacaat atacagcctg tatataccaa
      121 ggaagttgtc ttctatatat cgaattacac tggagaggtt aatgttaata aatctgagat
      181 agatgaagcc ggatggttta atatgtctga agctaagagg cgtttaagat atatggagtt
      241 atgctccgta ttagaagatg cggaagaata tataggtaat cttattgaaa attagttaaa
      301 tgaattttta atgaatttca ttaaaattta agttaattca ataatatttc atgtaaatta
      361 agaaaattta ataaaaatca gatttattta actaaagtat tgtatatttt agttatttat
      421 gctaagataa ctataaatgg aggtagctat gctggagagt gagttaatta gattagttaa
      481 aaaaattact acagaaaaat gtgaaaaaca aaatttagaa cttaagaaag cttctaatgg
      541 aactcctgaa aagctgtatt ccacattgtc aagtttctct aatcaaattg gtggtggtat
      601 tattgttttc ggcattgatg aaaatgaggg atatgacatt gctggtgttt atgatgctca
      661 ggaccttcag aaaaaagttg gtgaacaggc tttacaaatg gaacctgtag tcagaccatt
      721 attcacagtt gcacagataa atggtaagct gatagtcagt gcggaaatat cagaatgtga
      781 tatatttgat aaaccatgct tttataaagg gtctgggaga ctgcgcggtt catatatcag
      841 ggtgggtgac tccgaccagc ctatgacgga gtatgagata tacagttatg aagcatataa
      901 gcgtagaata cacgatgaat tgagaactgt tgataaagca accttaaaat atcttagcaa
      961 agacaggatt gcagaatatc tgataaaaat acgaagacag aaagcaaact tagttaatct
     1021 tagtgatgaa cgattgcttg aaacacaggg tatagttaag aatggactcc ttacaataac
     1081 tggactaatg ctttttggtg agtatcccca ggagttcttc ccgcagttaa gtgtaacagc
     1141 aatggcagta caaggtagag caataggcga gttaggtaaa gataatgaac gctttattga
     1201 taaccgacgt attgaaggaa ctattcctca aatgctggag gatgcattat catttatacg
     1261 aaggaacatg aagataaaaa caattattaa taaagatgga gttcgcgctg ataaatatga
     1321 atatccattg gtagctgtta gggaaattgt cttgaacgct cttattcatc gtgattacag
     1381 tatacataca gaacgctccc caataagact agttatgtat gatgacaggt tggaattaga
     1441 aaatccaggt ggcttgtatg gaaggataac agtaaatgat ttaggtaaag tagcagcaga
     1501 tacaagaaac ccatacatgg caggagcttt agagattcta gaaacagccg aaaatcgttt
     1561 ttccggtatt ccaactgtaa tatctgaact aaaaaaggct ggtatgccag ccccagtatt
     1621 tattgataaa agaggtgttt tcaaagttat tttctataaa gaagatgcca ttcaaaatag
     1681 tataagtgaa ttggagcaga tgattttaga atactgtgag actccaagga gcagagaaga
     1741 gcttgtagtg aaatttaaat ttgattctgt aagttacttt atgaagacat atatctatcc
     1801 tcttgttgaa aaaggaatgt taaaaatgat atttccagaa aaaccaagaa gcaagttcca
     1861 aaaatattac tcataattaa aatataagag aaagcggtgt tctggtaaat catggtaagg
     1921 gaatacgact tggaagaatc aattgatata atggtcaaaa gcataactga tatactacac
     1981 gacaattgtt tgtcagtata tctgtatggt tctgtttgtg aagacgattt cagattgggc
     2041 tggagtgata tagatattct gtgtctgact aaagcttcag taaggcagga agatgcttta
     2101 aggcttgtag atctgagaaa atcattaagt aatatgtatc ctgataatca atacttcagt
     2161 ttgttcgaag gctgtatatt atctctgaac tcattcatta acaagactga agatacttgt
     2221 gtttactggg gttcatccaa aagcaggatt acaacacgtt atgattttaa tagtttaagt
     2281 atgacacagc tcattaaaag cagtaagctt ttgtatggca aagacatact tcatattttt
     2341 acgcttcctt catatgatga attaagaaat gatgtaataa atcattacga aacaatcaga
     2401 aaatatgcca aatcaacctc aagaagcata cattcatatg gatggctgtt agacatatca
     2461 aggtgcatat atacaataca gacaggaaag ataatatcaa agacaagagc tggtgagtgg
     2521 gcattggaat ataatttgtg tccagtacaa gaacctttgg aaaaagcagt aagggttagg
     2581 aaggatccat ataaatatca ttctgaccag gagattcttg attattctga gggtttggga
     2641 gagcatatac agaagtatgc tgatgtacta gaagaagtta taaaatattc ataaaaagat
     2701 agatatttct aaatatgtta cataatgtat caaaaattaa ataatgtgtt gacattttag
     2761 ttatccggat ataaactgtg cttgagagga tggtaatctt tcatgtttac atcgttaaga
     2821 ttgaaaaatt tcaaatcatt tggtgatata tattttgatt tcagaaaaag caagaatgaa
     2881 gc
//
LOCUS       DAOSXM010000158         2746 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2111
            NODE_27961_length_2746_cov_9.752508, whole genome shotgun sequence.
ACCESSION   DAOSXM010000158 DAOSXM010000000
VERSION     DAOSXM010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864178
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2746)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2746)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:24:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,261
            CDSs (total)                      :: 2,229
            Genes (coding)                    :: 2,218
            CDSs (with protein)               :: 2,218
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 3 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2746
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27961_length_2746_cov_9.752508"
                     /isolate="AMR_MDS_2111"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            181..1122
                     /locus_tag="PLQ68_11215"
     CDS             181..1122
                     /locus_tag="PLQ68_11215"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HXK73261.1"
                     /translation="MGFEIILTVILIVVIVLFVLSFVGGFIMFDLTLKPKSDKNIFKK
                     KEKDITNEQKQLHEKLLSWTNDNMDSVWNISTFDNLNLKARYFKGTDEHKWVIVVHGY
                     TSVGWHMSAFAKEYLSKGYSVLMPDLRGHGISEGEYATMGYYDSVDIIEWIYEIIKKD
                     PEACIVLHGVSMGAATVMMVTGEDIPVHVKCVIEDCGYTSVWEVFKHNMKRMFKLPAF
                     PILYTANIISSAKIKMDFKEVSPLKAVSNSKTPILFIHGDQDVLVPFKMLNILYENAS
                     CEKDYLVIKEAVHGEASAKEPDTYYEKVSGFIDKYIN"
     gene            complement(1141..2628)
                     /locus_tag="PLQ68_11220"
     CDS             complement(1141..2628)
                     /locus_tag="PLQ68_11220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004620950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fucose isomerase"
                     /protein_id="HXK73262.1"
                     /translation="MNNAPNVKLGIVAVSRDCFPAELSKRRRIQVVKEYEKLNKTIYE
                     CPIIVENENDAIKALDDVRKNKVDALVIYLGNFGPEGPETMLAQKFGSPTMFCAASEE
                     NDTDSLNDNRGDAFCGMLNASYNLGLRNIKSYIPSYPVGYPEEVALMITRFESIARIL
                     IGIKNLKIFAFGPRPFNFLACNAPIKPLFDLGVEIQENSELDLYESFVKHEDDKRIPS
                     VIENMNNELCGRNNHPAVMERLAQFELTLLDWKDNNLEASKYAAFADKCWPAFQTMFK
                     FVPCYVNSRMAAMGYPVACETDIYGALSEYILYLAAESIPTLLDINNTVPRIMYEKHV
                     KTKYPYSNQDIFMGFHCGNTSTCNLNSGCGTLNYQKIMKRLIEPDTEPDVSRGTLEGT
                     LKPGNMTLFRLQSTADAKLRSYIAKGEVLDVDPKSFGSIGVIAIPEMARFYRHVLIEK
                     QFPHHAGVSFDDVSSILFDAMNMLNVEDISFNRPANMLYNTENPF"
BASE COUNT          896 a          421 c          475 g          954 t
ORIGIN      
        1 gattactatt gagtaccgcg cctctatcaa tattagtgga agtataacaa gcatactatc
       61 taatgtaaag catttttttc aaattttaag ttatgtaggt tatttttatg atttttagtg
      121 gttatatatt atttgtgttt atttattatg atataataaa gatataaagc ggagggcaaa
      181 atgggttttg agattattct tacagttata ttaattgttg ttatcgtttt atttgtttta
      241 tcttttgttg gtggttttat aatgtttgac ttaacgctta aaccgaaatc agataaaaac
      301 atctttaaaa agaaagaaaa agatattacg aatgaacaaa agcagttgca tgaaaaactg
      361 ttatcatgga ccaatgataa tatggattca gtatggaaca tatctacatt tgataattta
      421 aatttaaaag caagatattt taaaggaact gatgaacata aatgggtaat tgttgtacat
      481 gggtatacat cagtaggctg gcatatgtct gcatttgcaa aagagtattt atcaaaagga
      541 tattcagttt tgatgccaga tcttagaggt catggcatta gtgaaggcga gtatgcgact
      601 atgggttatt atgacagtgt tgacattata gaatggatat atgaaattat aaaaaaagat
      661 cctgaggcat gtattgtact gcatggagta tcaatggggg cagcaacagt tatgatggta
      721 acaggggagg atattccagt acatgtgaaa tgtgttattg aagattgcgg atatacatca
      781 gtatgggaag tttttaagca taatatgaaa agaatgttca aactcccagc ctttccaata
      841 ctatatactg ctaatataat ttcatctgca aagattaaaa tggattttaa ggaagtaagc
      901 cctttaaaag cagtttcaaa ctcaaagact ccaatactat ttatacatgg ggatcaagat
      961 gtcctggtcc cttttaagat gcttaatatt ctttatgaaa atgcatcatg tgaaaaagat
     1021 tacttagtta taaaagaagc tgttcatggt gaagcttctg caaaagaacc ggacacctat
     1081 tatgaaaaag tgtccggatt tattgataaa tatattaatt gaatttttat gctacataac
     1141 ttaaaaggga ttctcagtgt tatataacat attagcaggt ctgttgaaac ttatatcttc
     1201 aacattaagc atattcattg catcaaagag aatagaactt acatcatcaa acgatactcc
     1261 tgcatgatga gggaattgtt tttctataag tacatgacga tagaatcgag ccatttcagg
     1321 aatagcaata acaccaatac ttccaaatga ttttggatca acatctaaaa cctcgccttt
     1381 agcaatgtaa cttcttaatt ttgcatcagc agttgattgt aatctgaaca atgtcatatt
     1441 acctggtttc aaggttccct caagagttcc cctgcttacg tcaggttcag tatcaggttc
     1501 aataagacgt ttcattattt tctgataatt taaagtacca caacctgaat ttaaattaca
     1561 tgttgaagtg tttccgcaat ggaatcccat aaaaatatcc tgatttgaat atgggtattt
     1621 tgttttaacg tgcttttcat acattattct tggaactgta ttattgatat caagtaaagt
     1681 cggaattgat tcagcagcaa gataaagtat atattctgat aaagctccat atatatcagt
     1741 ttcacaagct actggataac ccattgctgc cattcgagag tttacatagc agggaacaaa
     1801 tttaaacata gtctggaaag caggccagca tttatccgca aaagctgcat acttagatgc
     1861 ttcaagatta ttatccttcc agtcaagtaa agtcaactcg aactgagcca gtctttccat
     1921 tacagcagga tgattgtttc ttccgcaaag ctcattattc atgttttcaa ttactgaagg
     1981 tatgcgtttg tcatcttcat gttttacgaa agattcataa aggtcaagtt cagaattttc
     2041 ctgtatttca accccaagat caaataaggg tttgataggt gcgttgcatg ctaaaaaatt
     2101 gaaaggtctt ggaccgaatg cgaatatttt caagtttttt atgcctatca atattcttgc
     2161 aatagattca aaacgagtaa tcattaaagc tacttcttca ggataaccta ctggatatga
     2221 aggtatgtat gatttaatat ttcttaaccc gagattatat gatgcattaa gcataccgca
     2281 aaaagcatca cctctgttat catttaaaga gtcagtatcg ttttcttctg atgctgcgca
     2341 gaacattgtt ggcgaaccga atttctgtgc aagcattgtt tcaggtcctt caggtccgaa
     2401 gttgccaaga tatataacta gcgcatctac cttgtttttt ctaacatcat caagtgcttt
     2461 tattgcatca ttttcatttt cgacgataat agggcactca tagatggtct tatttagttt
     2521 ttcatattct ttaactacct gaattcttct tctttttgat agttcagcag gaaaacagtc
     2581 acggcttacc gctactattc caagttttac atttggcgcg ttgttcattt cgacctccgt
     2641 tgatttgaat tacataataa ttttatactg tctcacagat ttagtaaaga actgaatata
     2701 tatatattat ttttacgaat ttatattatt tattgaattt ttatat
//
LOCUS       DAOSXM010000159         2418 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2111
            NODE_34369_length_2418_cov_15.389759, whole genome shotgun
            sequence.
ACCESSION   DAOSXM010000159 DAOSXM010000000
VERSION     DAOSXM010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864178
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2418)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2418)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:24:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,261
            CDSs (total)                      :: 2,229
            Genes (coding)                    :: 2,218
            CDSs (with protein)               :: 2,218
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 3 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2418
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34369_length_2418_cov_15.389759"
                     /isolate="AMR_MDS_2111"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            3..1526
                     /gene="istA"
                     /locus_tag="PLQ68_11225"
     CDS             3..1526
                     /gene="istA"
                     /locus_tag="PLQ68_11225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007658711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HXK73263.1"
                     /translation="MSKVRKVIQLYHQKQGKQFISKYLSISRNTVKKYIALYQLLNLS
                     IEDINQRSDAELEELFSNNKTQTLSPKLKEVYAFFPYMERELKKTGITKQRMWEEYFK
                     QHPDGLMSSQFCEHYNRWSKRVNPVMHMNHKAGDKMFIDYAGKTLEIVEKDTGEITEV
                     QFYVAILGASQLTYAEATPSQQKEDFIASTENAMHYFGGVPAAIVPDNLKSAVTKSNR
                     YEPTINETFLDFAEHYETTILPARAYKPRDKSLAEGVVKILYQRIYPVIRKQVFYSIK
                     DMNKAILEELEKHNNRKLTGRPQSRREMFNDIEKESLSPLPVNRYEIKQLAFATVMQN
                     GHVLLSKDKHYYSVPYNYIRKKIKIMYTSEKVEIYHKYNRIALHKRDMRLYNYTTVTE
                     HLATTHQFITEWTPQRFINWAASIDEDVKIYITNLLERKQHPEQAYKSCMGVLSFAKK
                     VGNERLIKACRRAIEFDTYNYKIIQNILENSLDMLIEEQQNKELPKHNNIRGENYYQ"
     gene            1543..2289
                     /gene="istB"
                     /locus_tag="PLQ68_11230"
     CDS             1543..2289
                     /gene="istB"
                     /locus_tag="PLQ68_11230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008616390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HXK73264.1"
                     /translation="MNEITLLRMKQMKLYGMHGAFKTAIETGKTDDYTLDQFVSMITD
                     AEWDDRTNRKIERMIKNARFHYKASIENIIYDEQRNIDQLKIHRLAECEYIERNENVL
                     ITGSTGVGKSYIATSLGYQACIQGYKVMYFNTTKLFSMLKIAKADGSYLKTLSKIERQ
                     DVIILDDFGLQPLDNQNRIALLEIIEDRNNNGSMIVTSQIPIKGWYEIIGEKTIADAI
                     LDRLIHQSHRLELEGESMRRKRKVINEEKN"
BASE COUNT          869 a          427 c          515 g          607 t
ORIGIN      
        1 atatgagtaa agtaaggaaa gtcattcaat tgtatcatca aaagcagggc aagcaattta
       61 tcagcaagta tttatctata tccaggaata ccgttaaaaa gtatattgct ctgtatcagt
      121 tattaaatct cagtattgag gacatcaatc agcgcagcga tgcggaactt gaggagttgt
      181 tcagcaataa taagacccaa accttgtctc caaaacttaa ggaagtttat gccttttttc
      241 catatatgga gcgggagctt aaaaagacag gcatcaccaa gcagcgtatg tgggaggagt
      301 acttcaagca gcatccagat gggttaatga gctctcagtt ttgtgaacat tacaataggt
      361 ggagcaagcg tgtaaatccg gttatgcaca tgaaccataa ggctggagat aagatgttta
      421 tagattatgc tgggaagact ctagagatag tagaaaaaga taccggagag atcactgaag
      481 ttcagtttta tgtagccatc ctgggggcca gtcagctcac ctatgccgaa gccacaccga
      541 gtcaacagaa agaggatttt attgcatcta cggaaaatgc catgcattat tttggaggag
      601 ttcctgcagc tattgttcct gataatctga agtctgcagt gacaaagagc aatcgatatg
      661 agcccacaat caatgagacc tttttagatt ttgcagaaca ctatgagaca accatacttc
      721 cagccagagc ttataaacca agagataaat ccctggcaga gggtgtggtg aagatcctgt
      781 atcagcgcat ctatcctgtg atacgaaagc aggtctttta tagcataaag gatatgaata
      841 aagctatact ggaagagctg gaaaagcata ataacaggaa gctcaccgga cgcccccagt
      901 cacgcagaga gatgtttaat gatatagaaa aagaatctct ttctccacta cctgtgaacc
      961 gctatgagat caagcaactt gcctttgcca cagtaatgca aaatggtcac gtcctgctaa
     1021 gtaaggataa gcactattac agtgtgccat ataactatat ccgcaagaag attaaaataa
     1081 tgtataccag cgagaaagtg gagatatatc ataaatacaa ccgcattgcc ctacacaaaa
     1141 gagatatgag gttatataat tataccactg tgacagaaca cctggcaacc acacatcagt
     1201 ttatcactga gtggactcca cagcgcttca ttaactgggc tgcctctatt gatgaagatg
     1261 tgaaaatata tataaccaat cttctggagc gaaaacagca cccggagcag gcttacaaaa
     1321 gttgtatggg tgttttgtct ttcgcaaaga aagtgggtaa tgaaaggctt ataaaggcat
     1381 gcagaagggc aattgaattt gatacctaca attataagat tatccagaat atcctggaaa
     1441 acagtctgga tatgcttatt gaagaacaac agaataagga acttccaaag cacaataaca
     1501 taagaggaga gaactattac caataacaat aaataaatga atatgaatga gattacattg
     1561 ttaagaatga agcagatgaa gctttacggc atgcatggag catttaaaac ggctatagaa
     1621 acaggaaaga cagatgacta tactttagac caatttgtat caatgattac tgatgcagaa
     1681 tgggatgaca ggaccaatcg aaagatagag agaatgatta agaatgcccg gttccactac
     1741 aaagcatcga ttgaaaacat catctatgat gaacaaagaa acattgatca gcttaagatc
     1801 catcgccttg ctgaatgcga gtatattgag agaaatgaaa atgtattaat cacgggaagt
     1861 accggagtag gtaaaagtta catcgccaca tcgcttggct atcaggcatg tatacaaggc
     1921 tacaaggtca tgtattttaa tacaacaaag ctattttcaa tgttaaagat cgccaaggca
     1981 gatggctcat atttaaaaac actttcaaaa atagaacgac aggatgtaat aatcctggat
     2041 gattttggat tacaaccact ggataatcaa aacagaatag cattgttaga aattatagaa
     2101 gatagaaata acaacggatc catgatcgtt acatcacaaa tcccaatcaa gggctggtat
     2161 gagattatcg gagaaaaaac cattgcagat gctatcctgg acaggctgat acatcaatct
     2221 catcgcctgg aacttgaagg ggaatccatg agaagaaaaa gaaaagtaat aaatgaagaa
     2281 aaaaactaaa tttgtttaac cacatgacag cgcaaaaaag gttctttttt tagtggaaaa
     2341 tcaggggtgg tcaatttgct ccggcggagg tggtcaattt aaattggaat tgggtggtca
     2401 atttgtccgg tttttcca
//
LOCUS       DAOSXM010000160         2372 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2111
            NODE_35465_length_2372_cov_10.737592, whole genome shotgun
            sequence.
ACCESSION   DAOSXM010000160 DAOSXM010000000
VERSION     DAOSXM010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864178
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2372)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2372)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:24:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,261
            CDSs (total)                      :: 2,229
            Genes (coding)                    :: 2,218
            CDSs (with protein)               :: 2,218
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 3 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2372
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35465_length_2372_cov_10.737592"
                     /isolate="AMR_MDS_2111"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            130..1716
                     /locus_tag="PLQ68_11235"
     CDS             130..1716
                     /locus_tag="PLQ68_11235"
                     /inference="COORDINATES: protein motif:HMM:NF024602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4038 domain-containing protein"
                     /protein_id="HXK73265.1"
                     /translation="MKELYKELVFTSKSDYKDFFNEVQFDVTFTSPDSEKFTVPGFWA
                     KDNVFKVRYDISIPGKHRYVTISNDKSLNGISGELVINKEKTDNPLYLHGPVYKKEQD
                     MHLSHKDGTAFYWLADTWWMALTKRLSFPKDFETLLNDRTEKGFNVVQIIAGLYPDMT
                     MFDPRGENEAGFPWKEDLSALNPEYFDKADDRIKMLVDKGVMPCIVGSWGFYMSFAGK
                     DVLKKHWRYIIARWSAMPVCWCVAGEANMAFYNDSVSYEDHIKQSRKDWNDMTVFIKQ
                     SDPYGRLITIHPFPATDGYDQVENGNLLDLNMLQTGHGGFSSLVNQMKKIKKAVDRKV
                     LPVINSEVCYEGICGSSYADTQRYIYLSDFLLGAAGHTYGANGIWQINSEEMPYGASP
                     HGAHWGNTSWKEAMNLDGSKHISLCMKYLTSFEWWKFEYHPEWVETPCTLEACDGHFA
                     AGIPKKVRVIYKPHFGGSFFGDINVLNIETDVSYSAYRFNPITGKETPLGHVIPDQEG
                     KWKFPRVDAFQDWIYVLKVM"
     gene            complement(1718..2203)
                     /locus_tag="PLQ68_11240"
     CDS             complement(1718..2203)
                     /locus_tag="PLQ68_11240"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010243389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HXK73266.1"
                     /translation="MEKKKVEIKAAVEPKSKVLVSVRDKDGKDNALVVAYCCNCSYAP
                     PMVMVGIVPSRYSYHMVKENPCFVVNLVSKNQNEIYKYLGSVSGRDEDKLAKINTKDG
                     DVVNAAILTDCPVNIECTVVDSIMTGSHEMFVGKIECVHADSDIINEKGEIDYSKIDF
                     I"
BASE COUNT          788 a          422 c          429 g          733 t
ORIGIN      
        1 aaatgggatt tctcttttat tttatcaatc cccaaacaag tttacactat cttatgataa
       61 tattagttga ctgcatgtta ttcaaagtat taatatttaa gtataaatat atacaaaaag
      121 gaaaattaaa tgaaagaact atacaaagaa ctggttttta catccaaatc cgattacaaa
      181 gacttcttta atgaagtgca atttgatgtt accttcacgt caccagatag tgaaaaattt
      241 acagttcctg gattctgggc aaaagataat gttttcaagg tcagatacga tatttccatt
      301 cctggaaaac accgctatgt cactatcagt aatgataaga gtttaaatgg aatatctggt
      361 gaacttgtca taaacaagga aaagacagat aaccctcttt atctgcatgg tccagtatac
      421 aaaaaagagc aggatatgca tctttcacat aaagacggta cagcttttta ctggcttgca
      481 gacacttggt ggatggcgct tacaaaaagg cttagttttc caaaagactt tgaaactctg
      541 cttaatgaca gaacagaaaa aggtttcaat gtagttcaga ttattgcagg actttatcct
      601 gacatgacaa tgtttgatcc aagaggcgag aatgaagctg gttttccttg gaaagaagat
      661 ttatcagcat taaatcctga gtattttgac aaagctgatg atcgaataaa aatgcttgta
      721 gacaaaggtg ttatgccatg tatagtaggt tcatggggct tctatatgtc ttttgcagga
      781 aaggatgttc ttaaaaagca ctggagatat attatagcaa gatggagcgc tatgccagtg
      841 tgctggtgtg ttgctggaga agccaatatg gctttctata acgacagcgt ttcttatgaa
      901 gaccatatta aacaatcaag aaaagactgg aatgacatga ccgttttcat taaacagtca
      961 gatccatatg gaaggctgat tacaattcac cctttccctg cgacggacgg atatgaccag
     1021 gtagaaaatg gtaatctttt agacctaaat atgcttcaga ccggccatgg tggatttagc
     1081 tcattagtaa accaaatgaa aaagataaag aaagctgtag acagaaaagt actacctgta
     1141 attaattcag aggtatgtta tgaagggata tgcggatcat catatgctga tacccagcga
     1201 tatatttatc tgtcagattt tcttttagga gctgcaggtc atacatatgg cgcaaatggc
     1261 atatggcaga taaattcgga agaaatgcca tatggagctt cacctcacgg agcccactgg
     1321 ggtaacacca gctggaaaga agcaatgaat cttgacggtt ccaaacatat atccctatgc
     1381 atgaaatacc tcacctcgtt tgaatggtgg aaattcgagt atcatcctga atgggttgaa
     1441 accccatgta cacttgaagc atgtgatggt catttcgccg caggaattcc aaaaaaagtc
     1501 cgagtgatat acaaacctca cttcggagga agtttctttg gagacataaa tgttcttaat
     1561 attgaaactg atgtgtctta ctcagcatac agatttaatc caataacagg caaagaaacc
     1621 cctcttggac atgttatacc agaccaagag ggaaaatgga aatttccaag ggtagatgcc
     1681 ttccaggact ggatatatgt ccttaaggtt atgtagatta aatgaaatct attttagaat
     1741 aatcaatctc gcctttttca tttataatat ctgaatcagc atgaacgcat tctattttgc
     1801 cgacaaacat ttcgtgcgat cctgtcataa ttgaatcaac tacagtacat tcaatattta
     1861 caggacaatc agtaagtatt gcggcattaa ctacatcacc gtctttagtg tttatttttg
     1921 ctaacttgtc ttcatctcta ccgctaacac taccaagata cttatatatc tcattctggt
     1981 ttttactcac aaggttaact acaaaacatg gattttcttt aaccatatga tatgaatacc
     2041 ttgaaggaac tatgccaacc ataaccattg gaggtgcata actgcaatta cagcagtaag
     2101 caactactaa tgcattatct ttaccatctt tatcccttac agatactagt acttttgatt
     2161 taggttcaac agctgcttta atttcaactt ttttcttttc cataataacc ttctttcatt
     2221 ttttaagtga aataatataa atatttacat ctataccaat atttaactat aagaatctta
     2281 tcacccataa ttttttatgt caatttgggt tgaagccatg atagagtcaa tttactgtag
     2341 ggtttgagca gatgctcttc ctgaaagcaa at
//
LOCUS       DAOSXM010000161         2369 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2111
            NODE_35538_length_2369_cov_10.381590, whole genome shotgun
            sequence.
ACCESSION   DAOSXM010000161 DAOSXM010000000
VERSION     DAOSXM010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864178
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2369)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2369)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:24:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,261
            CDSs (total)                      :: 2,229
            Genes (coding)                    :: 2,218
            CDSs (with protein)               :: 2,218
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 3 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2369
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35538_length_2369_cov_10.381590"
                     /isolate="AMR_MDS_2111"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            120..860
                     /locus_tag="PLQ68_11245"
     CDS             120..860
                     /locus_tag="PLQ68_11245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013282408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HXK73267.1"
                     /translation="MDLFAIIVVLIAYIIKGLSGFANALVFSSFMSFQNNNINISPIE
                     VLMAFPSNLILTVKERKNLSWKIFLPLIIMVIIGVIPGTILLKNIDATVLKIFFGFAI
                     ALISIEIFLREFGKNTKKVQSSPLALTIIGIISGVLSGLFGVGAFLVAYISRTTHNSS
                     AFKANLCIVFMVENIFRIIIYISTGILTLTIAKQALLLMPFMLIGLFIGILLSKKISE
                     SLIKKIIIILLLLTGISLMITNILVLLL"
     gene            complement(982..>2369)
                     /locus_tag="PLQ68_11250"
     CDS             complement(982..>2369)
                     /locus_tag="PLQ68_11250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014356127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HXK73268.1"
                     /translation="IISGSYPVMNESGIVIGTVSSGYRLDKFEMLDDTKFVVQTDLTI
                     FCDNIRISTTIENNGQRVVGTELDPVIANIVLNQKTDYTGEADILGNKFITYYLPIMD
                     ASNNSIGVLFAGKSMEQFNAELAEIIWFIAGSSLAAVIFVTIISMFIVKKTMTNPIKN
                     TAQLANELALGNLDHPINIKNKDEIGDLASILNVNVRDVFKRIEEVRVDIDNASKQVA
                     VGTQQLADGSQSISSGATEQASSLEQLTASVSQIAEQSNQNAISSNKANKLSMDAKNS
                     AVQGNDLMNDLQKAMQDINKSSLDISKIIKVIDDIAFQTNILALNAAVEAARAGIHGK
                     GFAVVAEEVRNLAAKSANAAKETTELIEGSIESVNSGTGIANKTAESLKSIVESVEEA
                     HKIVSEISSSSAEQANAVAQINNALDQLSKVVQQNSATAEQTAASSEELSSQADLLKN
                     MVELLKIKKEC"
BASE COUNT          740 a          418 c          342 g          869 t
ORIGIN      
        1 atatagacgt aaaatctgtt atgagcgagt tggcggaaat acagaaataa ttaaaatatt
       61 gttaaatttt ctttaaaaat gtataatgag ccataataaa ctttaaagta ggattataca
      121 tggatttatt cgccattata gtagtactaa ttgcatatat aattaagggt cttagcggtt
      181 ttgctaatgc cttggtattc agctcattta tgagttttca aaataataat atcaatattt
      241 caccaattga ggtgttaatg gcctttccat ctaatcttat tcttactgtt aaagaaagaa
      301 aaaatctttc atggaaaatt tttctgcctt taataataat ggtaataatc ggagtaattc
      361 caggaactat acttttaaaa aatattgatg caactgtact taaaatattt ttcggttttg
      421 caatcgcatt aatatcaatc gaaatctttc tacgtgaatt tggaaaaaat acaaaaaaag
      481 ttcaaagctc tcctttagct ctaactataa tcggaataat atcgggagta ttaagcggac
      541 tatttggtgt tggtgctttt ttagtagcat atataagtcg aacaacacat aactcaagtg
      601 cctttaaggc aaatttatgc attgtattta tggttgaaaa tatttttcgt atcatcattt
      661 atatatctac tggtatactt accttaacaa tcgcaaaaca agctttgtta ctcatgcctt
      721 ttatgctaat aggattattt ataggaattc ttttatctaa aaagataagt gaatctctaa
      781 taaagaaaat tatcataatt ctcttacttt tgactggaat ttcacttatg ataactaaca
      841 ttttagtgtt attactctaa tgctatagca agtaagaaaa atatttaaaa tgacattaac
      901 atttaaaata caaaaagccc ccgaaagagc ttcttgtatt cttgtatttt tcatctgttg
      961 ctcagatctt atcttatgta ctcagcattc tttcttaatc tttaacaact caaccatatt
     1021 tttcaaaaga tcagcctgac tagaaagttc ttcactagat gcagctgtct gttcagcggt
     1081 tgctgagttc tgctgtacaa cttttgatag ctgatcaaga gcattattaa tttgcgcaac
     1141 agcatttgcc tgttctgctg aggaggagga aatttcactg acaatcttgt gcgcttcttc
     1201 aacactttca acaatgctct taagcgattc tgctgtttta tttgctattc ccgttccgga
     1261 attcacactt tcaatagacc cttctattaa ttctgtagtc tcttttgctg catttgcact
     1321 ttttgcagca agatttctta cttcttctgc aacaacagca aatccttttc catgtattcc
     1381 agctcttgca gcttcaacag ctgcattaag agctagtata ttagtctgga atgctatatc
     1441 atcaataact tttattatct ttgatatatc aagtgatgat ttgtttatat cctgcattgc
     1501 tttctgcaaa tcattcatta ggtcattccc ctgtactgct gaatttttcg catccataga
     1561 aagtttattt gccttatttg aacttattgc attctgattt gattgttcag ctatttgaga
     1621 aacagaagct gttaactgtt caagtgaact tgcctgttct gttgcacctg acgatattga
     1681 ctggcttccg tcagctaact gctgagttcc aacagcaact tgttttgaag cattatctat
     1741 gtcaactcta acttcttcaa tccgtttaaa gacatctctt acatttacat tcaatatact
     1801 tgcaagatct cctatttcgt ctttattttt tatatttatc ggatgatcca gattgcctag
     1861 cgcaagttca tttgcaagct gtgctgtatt ttttattgga ttggtcattg tcttttttac
     1921 aatgaacata gaaataatag taacaaatat tactgcagcc aaagaagaac cagcaataaa
     1981 ccagattatc tctgccagct cagcattaaa ctgttccatt gattttcctg caaagagtac
     2041 acctattgaa ttattacttg catccattat tggcaaatag taggttataa atttgttacc
     2101 cagaatatca gcttctcctg tatagtcagt cttttgattt aaaacaatat tagctataac
     2161 aggatctaat tcagttccaa ctactctttg accattattt tcaatagtag tagatatgcg
     2221 aatattatcg cagaatattg ttaaatctgt ctgaacaacg aatttggtat catcaagcat
     2281 ttcaaactta tcaagcctat accctgaaga aactgttcca ataacaatac cagattcatt
     2341 cataacagga taagaaccac ttattatta
//
LOCUS       DAOSXM010000162         2222 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2111
            NODE_39307_length_2222_cov_7.507614, whole genome shotgun sequence.
ACCESSION   DAOSXM010000162 DAOSXM010000000
VERSION     DAOSXM010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864178
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2222)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2222)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:24:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,261
            CDSs (total)                      :: 2,229
            Genes (coding)                    :: 2,218
            CDSs (with protein)               :: 2,218
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 3 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2222
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39307_length_2222_cov_7.507614"
                     /isolate="AMR_MDS_2111"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..917
                     /locus_tag="PLQ68_11255"
     CDS             <1..917
                     /locus_tag="PLQ68_11255"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HXK73269.1"
                     /translation="SKVIYKSLKNIIQPYNSNTDYVKVRQDLENLAKLQNINRKVELT
                     CIPLKNCTMEMVSVPEAPKDKIIMYIHGGGFTTGGIVTSRKFITEFCLRAKLNAVSCD
                     YRLAPENSFPDPLDDCVAMYEHLLSMGYSSQNIVLLGESAGGTLTLALTLLLKDNSKP
                     LPAAICSMCPVGDMTGTLDSRERNIKVEALLRYDLDEKIRMAYVNDYDVCDPYISPIY
                     GNYENFPPLLLQLGEYEILYDDSVLLYEIAKEKGVDVTLSVWDKMWHGFQIIWGMPES
                     KLAHKEIIEFFHKHLGYDEYEEYRKKYN"
     gene            1026..2156
                     /locus_tag="PLQ68_11260"
     CDS             1026..2156
                     /locus_tag="PLQ68_11260"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HXK73270.1"
                     /translation="MFKPDYTVLRKKSEFVWYVNMSYYAMAELADVTLHDLFTNYKAS
                     LKLYSKENFDKFNKHFPMHEKLRKLNPSTPPISYGHINGLGIDLVFPEGNGEVNYKRC
                     DKSLDDWISILDKPIDFSKAGMAPYYLEYQDNMKKAMNVDKVNFSYGYEGPITTAYTM
                     LDLKLYYDLYDDPEKLKIFLEKLSWSITQFKRFHAHVNGNDYKNTSYMCDDCATLVSA
                     DMWEEFVIPYMDIVYSDVNANRRSLHSEGMIYKHLKNLEKLGITWFDPSVSPKLDPEI
                     IYKNCEVPFIWRMCSIYHSLLDRELAMDFVFSAVRDGASGLFTTARDLEEHNINVVEG
                     FIQACETVQDMFAKGADRSDVGKLVSDRGKDIFWEKWRTQYI"
BASE COUNT          778 a          304 c          438 g          702 t
ORIGIN      
        1 tatcaaaggt tatttataaa tctttaaaaa atattattca gccatataac agtaatactg
       61 attatgtcaa agttcgtcag gatttagaaa atctggctaa gttgcagaat atcaatagaa
      121 aagttgaact tacctgtata cctttgaaaa actgcactat ggaaatggta tcagttcctg
      181 aagctcctaa agacaagata attatgtata tacacggtgg tggttttact actggtggaa
      241 tagttacatc aaggaaattt attacagaat tttgtttaag ggctaagctc aatgctgtaa
      301 gctgcgatta caggcttgcc ccggaaaatt cttttccaga cccgctagac gattgtgtgg
      361 caatgtacga gcatcttcta agtatgggat acagtagtca gaatatcgtt cttttaggtg
      421 agagtgcagg tggaacgctt acattagcac tgactttgtt acttaaggat aatagtaagc
      481 ctttaccagc tgcaatatgt tctatgtgtc ctgtaggaga catgacagga acacttgaca
      541 gcagagagcg aaatattaag gttgaagctt tattaagata tgatcttgat gaaaaaataa
      601 ggatggcata tgtcaatgac tatgatgtct gtgatccata tatttcaccg atatatggaa
      661 attatgaaaa ttttccacca cttttgcttc aactgggaga atatgaaata ctttatgatg
      721 attctgtttt gctgtatgag atagcaaagg aaaagggtgt agatgttact ttatcagttt
      781 gggataagat gtggcacgga tttcagataa tctggggtat gccagagtca aagcttgctc
      841 acaaagaaat aattgaattt tttcataagc atttaggata tgatgaatat gaagaataca
      901 ggaaaaagta taattaattt tttaaataaa tcagtattta attaattaaa cgggttgaat
      961 accttggtaa taaggtataa ttaaacaagg caccataaat gccataaaaa ctgaagaggt
     1021 gatttatgtt taaaccggat tatacagttc ttagaaaaaa atcagaattc gtgtggtatg
     1081 taaatatgtc atactatgct atggctgaac ttgctgatgt tacgttacat gacttgttta
     1141 caaattataa agcatcatta aagctttatt caaaagaaaa ttttgacaag tttaataagc
     1201 attttcctat gcatgaaaaa ttaaggaaat tgaacccttc aactcctcct atatcatatg
     1261 gccatattaa cggactaggg attgatttag tttttcctga aggtaatggg gaagttaatt
     1321 ataaaaggtg tgataaatca cttgatgatt ggataagcat acttgataaa ccaatcgatt
     1381 tttctaaagc aggaatggct ccatattatc ttgagtatca ggacaatatg aaaaaggcaa
     1441 tgaatgtaga caaagtaaat ttcagttatg gatatgaagg ccctataaca acagcatata
     1501 caatgctgga tttgaagctt tattatgact tatatgatga cccggaaaag ttaaaaatct
     1561 ttctggaaaa actttcatgg agtataacac agttcaagag attccacgct catgtaaacg
     1621 gtaatgatta caagaacaca tcatacatgt gtgatgactg tgcaacactt gtttcagcag
     1681 atatgtggga agaatttgtt attccttata tggacattgt atactctgat gtaaatgcta
     1741 acagacgttc attgcattca gaaggtatga tatacaaaca cttgaaaaat cttgaaaaac
     1801 taggtattac atggtttgac ccatcagtat cgccaaaact tgacccagaa attatttata
     1861 aaaactgcga agtaccattt atctggagaa tgtgctcaat atatcacagt cttttagatc
     1921 gtgaacttgc tatggatttt gtattttctg ctgtaagaga tggggccagc ggattgttta
     1981 caacagcaag agacctagaa gaacataaca taaatgttgt tgagggtttt atacaagcat
     2041 gtgaaactgt tcaggatatg tttgctaaag gagcagatag atctgatgtt ggaaagttag
     2101 tttcggacag aggaaaagat atattctggg aaaaatggcg tacgcaatat atttaataag
     2161 tgtaaataca gctgtattga ttttattaag aaaggtgata taatttacat aaattagtta
     2221 tt
//
LOCUS       DAOSXM010000163         2103 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2111
            NODE_43001_length_2103_cov_11.351074, whole genome shotgun
            sequence.
ACCESSION   DAOSXM010000163 DAOSXM010000000
VERSION     DAOSXM010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864178
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2103)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2103)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:24:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,261
            CDSs (total)                      :: 2,229
            Genes (coding)                    :: 2,218
            CDSs (with protein)               :: 2,218
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 3 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2103
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43001_length_2103_cov_11.351074"
                     /isolate="AMR_MDS_2111"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..897
                     /locus_tag="PLQ68_11265"
     CDS             <1..897
                     /locus_tag="PLQ68_11265"
                     /inference="COORDINATES: protein motif:HMM:NF017263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HXK73271.1"
                     /translation="GESNPDAFMYHRFAIRKTRTAISHLTRAYMHTKDEIYAEKAVEL
                     LKVFFINEDTKMMPHLLYAQALPGICSGRGIGIIDTLHITDIPFALEVLKKSNSLTND
                     IYNKMVRWFEDYLEWMTTHQHGIDESNEPNNHGICYYVQVAVFALFTENTHLLEHCRE
                     MFFKKVIVQMNKDGGFDKELARTKPYGYSLFVLDNFITLCHVLSDSKNNFWEYKTTDG
                     RCARKAIEFMIPYIKDKEIWPYRKDVEYFNDYPSRMSFMIFGGYVLGIKELIQIYQSL
                     PEESYVDEVRRNTTIRQPFLWI"
     gene            complement(900..1688)
                     /locus_tag="PLQ68_11270"
     CDS             complement(900..1688)
                     /locus_tag="PLQ68_11270"
                     /inference="COORDINATES: protein motif:HMM:NF016816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HXK73272.1"
                     /translation="MNIYVMVDAEGLCGIFSKEQVSPEGSRYNECREHMTREVNIVVE
                     ALKEAGVERVYVRDAHSAGNNVYWDKLSDKAYRYIVGNTGTDRFPGLDDCDGVILLGY
                     HAMAGTRGALLEHSMSSSRVQNYWINDMPAGEVAIDAGIAGDKNIPVIMVSGDDYLCK
                     EVKQLLPWAVACEVKKAASLYGIMTPPMNKAFDILREGVKRAVDNIDRMNPLVFDKPI
                     TFKVEVMERCPIPNSYAKPYLKIIDGRTYEITAGSMEEALFRSY"
     gene            complement(1792..>2103)
                     /locus_tag="PLQ68_11275"
     CDS             complement(1792..>2103)
                     /locus_tag="PLQ68_11275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK73273.1"
                     /translation="GNWDGQQIIPEQWVLDSTAKKIEISETQDYGYLFSLEKITDTIN
                     DKTYSMYQAYGSGGQIIMVIPELDLLIVISCNASISPTVKPMTRDIITDYILPSVYVI
                     E"
BASE COUNT          698 a          414 c          330 g          661 t
ORIGIN      
        1 ggagaaagca atcctgatgc atttatgtat catagatttg ctattagaaa gacaagaaca
       61 gcaatttctc atcttacaag agcatatatg cataccaaag atgagattta tgctgagaaa
      121 gcagtagaac ttctaaaagt gtttttcata aatgaagata caaaaatgat gcctcatctt
      181 ctctatgcac aggctcttcc tggcatatgc tcaggaagag gtataggaat tattgacact
      241 ttacatataa cggacatacc ttttgcttta gaggttttaa aaaaaagcaa ctctctaaca
      301 aatgatatat acaataagat ggttagatgg tttgaagatt atcttgaatg gatgactact
      361 caccaacatg gaattgatga gtcaaatgaa cctaataacc atggtatatg ctactatgtc
      421 caggttgccg tatttgctct ttttacagaa aacacccact tacttgagca ttgccgtgaa
      481 atgtttttca aaaaagtaat agtacagatg aataaagatg gcggattcga caaagaacta
      541 gcaagaacaa agccctatgg atattcattg ttcgtattgg acaatttcat aactctatgt
      601 catgtgcttt ctgatagtaa gaataacttc tgggaataca aaacaacaga tggaagatgt
      661 gccagaaaag ccattgaatt catgattcct tatataaagg acaaagaaat ctggccatat
      721 agaaaagatg ttgagtattt taacgattat ccttccagaa tgtcttttat gatttttgga
      781 ggatatgtat taggaattaa agaactgata caaatatatc agtcccttcc agaagaatct
      841 tatgttgatg aagtcaggcg aaacactact atccgtcagc catttttatg gatttaatat
      901 caatatgatc tgaataatgc ctcttccata ctaccggcag ttatttcata tgtccttcca
      961 tcaataatct ttaaatatgg ctttgcatat gaattaggta ttggacatct ttccataact
     1021 tcaaccttga aagttattgg cttgtcaaaa acaagaggat tcatcctatc aatattatca
     1081 actgctcttt taacaccttc tcttaatata tcaaatgctt tattcatagg aggagtcatg
     1141 attccgtata atgaagcagc ttttttaacc tcacaggcta ccgcccaggg caaaagctgt
     1201 ttaacctctt tacataaata atcatctccg cttaccatga ttaccggaat attcttatca
     1261 cctgctatac cagcatcaat agcaacttca ccggcaggca tatcattgat ccagtaattt
     1321 tgaactcttg aagaactcat ggagtgctct aaaagagctc ctcttgttcc tgccatcgca
     1381 tgatatccta agagaataac accatcacag tcatcaaggc ctgggaaacg gtcagtaccg
     1441 gtgttaccga ctatatatct atatgcttta tcagataatt tatcccagta aacattattg
     1501 ccagcagaat gggcatccct tacataaacc ctttcaacac cagcttcctt aagagcttca
     1561 actactatat taacttctct tgtcatatgt tctctgcact cattgtacct tgaaccttca
     1621 ggacttacct gttctttgct gaatataccg caaagacctt cagcatctac cattacataa
     1681 atattcatat tctacctcca gattctattc tcgattcact aataacagat atataatatg
     1741 atatcatgaa aaagcataaa tagcagatta ttaacgccaa aagaacacaa tttactcaat
     1801 tacataaaca gaaggaagta tatagtcagt tataatatct cttgtcatag gttttacagt
     1861 aggcgaaatt gaagcgttgc atgatattac aataagcaag tccaattcag gtatcaccat
     1921 aataatctgc cctcctgaac catatgcctg atacattgaa tatgtcttat cgttaattgt
     1981 atcggttatc ttttcaagcg aaaaaagata cccataatcc tgcgtttcgc ttatctctat
     2041 ttttttagca gtagaatcta gaacccattg ttcaggtatt atctgctgtc catcccagtt
     2101 acc
//
LOCUS       DAOSXM010000164         1882 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2111
            NODE_51753_length_1882_cov_11.930487, whole genome shotgun
            sequence.
ACCESSION   DAOSXM010000164 DAOSXM010000000
VERSION     DAOSXM010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864178
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1882)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1882)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:24:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,261
            CDSs (total)                      :: 2,229
            Genes (coding)                    :: 2,218
            CDSs (with protein)               :: 2,218
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 3 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1882
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51753_length_1882_cov_11.930487"
                     /isolate="AMR_MDS_2111"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..897
                     /locus_tag="PLQ68_11280"
     CDS             <1..897
                     /locus_tag="PLQ68_11280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK73274.1"
                     /translation="TITFRTDFDDIAFNKRRQSDRSLIKGRFQGGNIGWIEADQIELF
                     ACAYKKDIAKMTYPQTVIRELLEHMGPLNIHQMKEETKMLVKDITPVLHQLQQAFIIY
                     EDQYDGEWDRSWYLFEEMFPDADLNRYTKNEAVKILIKRFTYRNVAINEDMIKSFYKF
                     SKKEILTAINEMVLENILVKYDSGYILKEDMEYLMNRKPEKVKSAYAMQRNDFLVKSN
                     EYWLKEKFKSDEYETLQYLLIDGQFHGAAFGKFRYGPNDLEDIVLDLNDEIATERKDE
                     ILQAITDIRVNKKANRYIGKPV"
     gene            complement(902..1789)
                     /locus_tag="PLQ68_11285"
     CDS             complement(902..1789)
                     /locus_tag="PLQ68_11285"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXK73275.1"
                     /translation="MTVRFRNYTQQAGITDDYYKVRAFLVKLGYSEFVYARWDWMATH
                     SYLDKSAVGKIGLWEDNGMLVGIATFDTILGNAYCLAFPDYSYLKKDMLIYSKSNLRG
                     EKGFGIVIPDTDLLFQDIAAELGFIATPDKENDSVFYIDKTSTSYTLPSGFRITSMKD
                     TYDRYKYRSVLWKGFNHELKGEGELKYSIETDKEVDNEMVRPFVDLNLKIAVVNKEGD
                     FVSYCGMWYEPETGFAIIEPVATDPAYRKAGLGKAAVLEGMKRVGQLGAKIAFVGSHQ
                     QFYYSIGLRPYATSTVWEE"
BASE COUNT          647 a          333 c          342 g          560 t
ORIGIN      
        1 acaattacat ttcgtacaga ctttgacgat attgcgttta ataaaagaag acaatccgac
       61 aggagcctta taaaaggcag attccagggt ggtaatatag gctggataga agcagaccag
      121 atagaactat tcgcctgtgc atataaaaaa gatattgcta aaatgactta tcctcagact
      181 gtgattcgtg agcttttaga acatatggga cctttgaaca tacatcagat gaaggaagaa
      241 actaaaatgc tggtaaagga tataacacca gtcctgcatc aattgcagca ggcttttatc
      301 atatatgaag accagtatga cggggaatgg gaccgttcat ggtatctgtt cgaagagatg
      361 tttcctgatg cggatttgaa cagatatacc aaaaacgaag ctgtaaagat acttataaaa
      421 agattcacat acagaaatgt tgccattaat gaagatatga taaaatcttt ctataaattc
      481 agtaaaaaag aaatactaac agccattaat gagatggttt tagaaaacat actggttaaa
      541 tatgatagcg gatatatctt aaaagaagat atggagtatt taatgaatag gaagccagaa
      601 aaggtaaaat cagcatatgc tatgcagaga aatgatttct tagttaaatc caatgaatac
      661 tggctaaaag aaaagtttaa gtcagatgaa tatgagactc ttcagtacct tctcatcgat
      721 gggcaattcc atggagctgc attcgggaaa ttcaggtatg gacctaatga tttggaagac
      781 attgttctgg acttgaatga tgaaatcgca acagagagaa aagacgagat attacaggcg
      841 attacagata tacgggttaa taagaaagct aacaggtata taggtaaacc agtatagctg
      901 tttactcttc ccagacagta gaagtcgcat atggccttaa tccaatacta tagtagaact
      961 gctggtgaga gccgacaaaa gcaatcttcg caccaagctg tcctactctt ttcatacctt
     1021 ccaaaacagc tgcttttcca agccctgctt ttctgtaagc ggggtctgtt gctacaggtt
     1081 cgataatagc aaaacctgtt tctggttcat accacatacc acagtatgaa acgaaatctc
     1141 cctctttatt tactacagca atcttcaggt taaggtcaac aaagggacgt accatttcat
     1201 tatcaacttc cttatcagtt tctatagaat attttagttc tccttcacct ttaagttcat
     1261 gattaaagcc tttccacaga actgacctgt atttatacct gtcatatgta tctttcatgg
     1321 aagttattct gaaacctgaa ggaagtgtat atgaagtaga agttttatct atatagaaga
     1381 cagaatcgtt ttctttgtca ggtgtagcaa taaatccaag ttctgcagct atatcctgaa
     1441 acagtaaatc tgtatcaggt atgactattc cgaaaccttt ttctcctctt aggttggatt
     1501 ttgaatatat aagcatatct ttctttaggt aagaataatc agggaatgct aaacagtatg
     1561 cgttacctaa gatagtatca aatgttgcaa tacctacaag cataccatta tcttcccata
     1621 agcctatttt acctacagcg cttttatcta agtaactgtg tgtcgccatc cagtcccatc
     1681 ttgcatatac aaactctgaa taacctagtt tcacaagaaa agccctgacc ttataataat
     1741 catcagttat gcctgcctgt tgtgtatagt ttctgaatct gactgtcatg atgtcacctc
     1801 tttataaata aatggtatat agatataata ccacttagat agaaaagtga ttatagcaaa
     1861 atatgcaaaa acattaatta ag
//
LOCUS       DAOSXM010000165         1829 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2111
            NODE_54284_length_1829_cov_10.758174, whole genome shotgun
            sequence.
ACCESSION   DAOSXM010000165 DAOSXM010000000
VERSION     DAOSXM010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864178
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1829)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1829)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:24:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,261
            CDSs (total)                      :: 2,229
            Genes (coding)                    :: 2,218
            CDSs (with protein)               :: 2,218
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 3 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1829
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54284_length_1829_cov_10.758174"
                     /isolate="AMR_MDS_2111"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(128..1315)
                     /locus_tag="PLQ68_11290"
     CDS             complement(128..1315)
                     /locus_tag="PLQ68_11290"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HXK73276.1"
                     /translation="MSGTLLHRAVIGFARLIYDPYLRRKYKYDFEKVDYSKGNFIALA
                     NHTTNLDSMILGISFKQHMYFVAGEQLFRMGFVSTLLRVFLNPIKRMKAKTEAKTAVN
                     MLKALKAGHNICMFPEGSCTWNGETSNIIQSTAKLIKRAKVALITYRVEGGYPTMPRW
                     GRTIRVGKMTGRKANIYEPEQLEQMSIEQIYSAIREDLYVNYKNMSDKYEYIGKAPAE
                     YLETALFICPKCKGISTYTSKDDMFSCTCGVRLKYDSHCNLVDIDNNDFPFTTVLEWD
                     NWQRQWIIDNLPSLKQDNDKIITSDEEQDLFTYTESGTTSHILSGSMALYPDRLIFKD
                     KKTKNELVIKLEDITDIAIITRTTLTFSCSDMHYEIKCKTPRSALKYLLFCINLSPMR
                     YIV"
     gene            complement(1430..>1829)
                     /locus_tag="PLQ68_11295"
     CDS             complement(1430..>1829)
                     /locus_tag="PLQ68_11295"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020072487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HXK73277.1"
                     /translation="KVAFICVHNSCRSQIAEALGKHFASDVFDSYSAGTETVPKINQD
                     AVRLMKELYGIDMEKTQKSKLVSELPSIDAVVTMGCNVDCPYIPSRYRTDWGLEDPTG
                     KDDTEFLKVIKIIEEKIFELKEMIKNKVIL"
BASE COUNT          544 a          347 c          262 g          676 t
ORIGIN      
        1 gtacttcctt ccttcaaaat accttttatc caacactcta attttaaaat atacgtatat
       61 atttgtcaat taaagtgaat catatgattg aacctcatat gattgggaat ttttaggtta
      121 actcaaatta gacaatatat ctcatgggag aaaggtttat gcaaaacaac agatacttca
      181 gagctgacct tggcgtcttg cactttattt catagtgcat atcagaacag gaaaaagtaa
      241 gcgttgttct tgtaataatt gctatatcag ttatatcttc cagctttata actaattcat
      301 ttttcgtttt cttgtctttg aatattaatc tgtcaggata aagagccatt gaaccggaaa
      361 gtatatggct ggtagttcca ctttcagtat aagtaaataa atcctgttct tcatcagaag
      421 taattatttt atcattgtct tgtttcaaag aaggtagatt atctataatc cattgtctct
      481 gccaattatc ccattccaga actgtagtaa atggaaaatc gttgttatca atgtctacaa
      541 gattgcaatg cgaatcatac ttcagccgga caccgcatgt gcaactgaac atatcatctt
      601 tggaagtata tgtgcttata cccttacact ttggacatat aaataacgct gtttcaagat
      661 actcagcagg ggctttaccg atatattcat atttatcaga catatttttg taatttacat
      721 aaagatcttc acgaatagct gaatatatct gttctatgct catctgttct aactgttcag
      781 gttcgtatat atttgctttt cttccggtca ttttgcctac tctgatagtt cttccccacc
      841 taggcattgt aggatatccg ccttcaaccc tgtaagttat aagggctact tttgctcttt
      901 taataagttt tgctgttgat tggataatat ttgaagtctc tccattccat gtacagcttc
      961 cttcaggaaa catgcatata ttatgtcccg ctttaagcgc ttttaacata ttgacagctg
     1021 tttttgcttc agttttagct ttcattcttt taatgggatt taagaatact cttaaaagcg
     1081 ttgaaacaaa tcccatacgg aaaagctgct ctcctgcaac aaaatacatg tgctgcttaa
     1141 atgaaattcc taaaatcata ctgtcaaggt ttgtagtatg atttgctaaa gcaataaaat
     1201 taccttttga gtaatcaact ttttcaaaat catacttata ttttcttcta agatacgggt
     1261 catatattaa tctggcaaat cctataacag ctctgtgaag taatgtaccg ctcatattcc
     1321 ccccttaaat acattttacc ctttatatat tttgattaca acataatgta tcgcatataa
     1381 gcatattaca gcatatttat gaatttattg ttaatgaatt tattatccac taaagaataa
     1441 ctttattctt tatcatttct ttcagttcaa atatcttttc ttcaattatc tttatgactt
     1501 tcagaaattc agtatcatct tttccggtag gatcctctaa accccagtct gtcctgtatc
     1561 tgctcggtat atatggacaa tctacattac atcccattgt tactactgca tcaatactgg
     1621 gaagttctga aactagtttt gatttttggg ttttctccat atcaatgcca tacagctctt
     1681 tcattaacct tacggcatcc tgatttattt ttggaacagt ttcagtacct gctgaataac
     1741 tatcaaacac atcagatgcg aaatgctttc ccaatgcttc tgctatttgg gatctgcatg
     1801 aattgtgcac acagataaat gcaacttta
//
LOCUS       DAOSXM010000166         1620 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2111
            NODE_66422_length_1620_cov_12.888179, whole genome shotgun
            sequence.
ACCESSION   DAOSXM010000166 DAOSXM010000000
VERSION     DAOSXM010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864178
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1620)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1620)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:24:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,261
            CDSs (total)                      :: 2,229
            Genes (coding)                    :: 2,218
            CDSs (with protein)               :: 2,218
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 3 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1620
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66422_length_1620_cov_12.888179"
                     /isolate="AMR_MDS_2111"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..758
                     /locus_tag="PLQ68_11300"
     CDS             <1..758
                     /locus_tag="PLQ68_11300"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HXK73278.1"
                     /translation="VSSYRLFAVLAVIFGVITLSLLLYRNKIGIYKVIALIIPLLVFF
                     FTGARLFNLFLNPKAYETVSVFKLEFVGLSIFGGFIGAVFAVILWCKIFHIPVWRILD
                     LCIIPAAGSFAIARVGCFLNGCCSGKFFNSIFAVVYPRFINSTGLESYPVLPTQLFEL
                     ILALVVLIPALIVFKKKKVFPNGSAFLIYASLFCAMRLIILPLRRLPYEPWIKLYFYP
                     AFYIILIITLLAIFSIRYYMKVKGKAENNEMKS"
     gene            complement(875..1552)
                     /locus_tag="PLQ68_11305"
     CDS             complement(875..1552)
                     /locus_tag="PLQ68_11305"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006575250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0098869 - cellular oxidant detoxification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HXK73279.1"
                     /translation="MDENNVVRMPLLGDDAPAFTAVTTQGPINFPADYKGKWVILFSH
                     PADFTPVCTTEFMTFGSMIEEFKELNTELVGLSVDSLYAHIAWLRRIQELEWNGKKNI
                     EIKFPLIEDIRMEVSKLYGMVQPGQSNTQAVRAVFVIDPKGKVRTILYYPLSMGRNFD
                     EIKRIIQALQKADSDQCATPADWRPGDDVIVPTAGSCGTAKERMESKDEDMYCLDWFL
                     CFRKQSK"
BASE COUNT          461 a          270 c          284 g          605 t
ORIGIN      
        1 cagttagctc ttaccgttta tttgcggttt tagcagttat attcggagtt attacccttt
       61 ctttgctttt atatagaaat aagataggta tttataaagt aatagctctt attatcccct
      121 tacttgtatt cttttttaca ggcgcaaggc tctttaattt atttcttaat cccaaagcat
      181 atgagactgt ttctgttttt aagttagagt ttgtagggtt atcaatattc ggagggttta
      241 ttggagcagt ttttgctgta atactatggt gcaaaatatt tcatatacct gtgtggagaa
      301 tacttgattt atgtattata cctgcagctg gttcatttgc tatagcaaga gtaggatgct
      361 ttttaaacgg ttgctgttca ggaaaatttt ttaacagtat atttgcagtt gtatatccaa
      421 gatttataaa ttctactggt cttgaaagtt atccggtatt gcctacacaa ctttttgagt
      481 tgattctggc tctggttgta ctcattcctg ctttgattgt atttaaaaaa aagaaggtat
      541 ttccaaatgg aagtgctttt ttgatttatg cgtcgctatt ttgcgctatg cggctgatta
      601 tcttaccttt acgaagatta ccgtatgaac cctggataaa actatacttt tatccggcct
      661 tctatataat cttgataatt actttacttg caatcttttc tatcaggtat tatatgaagg
      721 tgaagggcaa agcagagaat aatgagatga aaagttaaaa ttataaaaga actattattc
      781 agttatttta attaagatat ataataataa atattatata aagaaagaac aggaataatt
      841 cctgttcttt tccttaagta tgatttggtt ttgcttactt gctttgtttt ctgaagcaaa
      901 ggaaccagtc aagacagtac atgtcttcat cttttgattc cattctttcc ttagctgttc
      961 cacatgaacc agctgttggc actataacat catcaccagg tctccagtct gctggggtag
     1021 cgcactgatc agagtcagct ttctgtaatg cctgaatgat acgcttgatt tcatcgaagt
     1081 ttcttcccat tgataaagga taataaagta ttgtacgtac ttttccctta ggatcaatta
     1141 caaatactgc acgtactgcc tgagtatttg attgccctgg ctgaaccatc ccataaagtt
     1201 ttgagacttc catacggatg tcttcaatga gagggaattt gatttcaata ttcttttttc
     1261 cattccattc aagttcctga attctacgaa gccaagctat gtgggcatat agagaatcaa
     1321 ctgaaagtcc tacaagttca gtatttaatt ctttaaattc ttcaatcatg gatccgaaag
     1381 tcatgaactc agttgtacaa actggtgtga aatctgctgg atgtgagaag agtattaccc
     1441 attttccttt gtagtctgca gggaagttaa ttggtccttg tgttgtaact gctgtaaaag
     1501 caggtgcatc atcacctaat aaaggcattc ttactacatt gttttcgtcc attgtttaat
     1561 tcctcctatg tgttaattat actatgtact aattatacat tcaactttat ccaaaaaatg
//
